LOCUS       DAOMAA010000001        23791 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_955_length_23791_cov_7.255772, whole genome shotgun sequence.
ACCESSION   DAOMAA010000001 DAOMAA010000000
VERSION     DAOMAA010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 23791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23791
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_955_length_23791_cov_7.255772"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="PLO59_00005"
     CDS             <1..231
                     /locus_tag="PLO59_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99499.1"
                     /translation="PHHSICILLLVSYLKFRFNNPARTAYEMTLTNTTEKKGEVLTAV
                     LRNGGCSGKLNICASIIISAKLKVSALKRHRA"
     gene            complement(268..963)
                     /locus_tag="PLO59_00010"
     CDS             complement(268..963)
                     /locus_tag="PLO59_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99500.1"
                     /translation="MWSYYGSKSKMVGCYPPPKFDKVIEPFAGCAKYSLKYFEKQVTI
                     VDKYKVLIDTWKYLQQASENDILKLPKLKRGDSIKDHKYLSEVEQNFLGFLVCNGLES
                     PRMNVSSFEGVNVERDLKRIAKSLFKIRHWNIILGTYEDIENVEATYFIDPPYMFGGE
                     HYKESSKNINFENLAEWCKSRNGQVIVCENTKATWLPFLPIKTMSGSMYKTTEAIWSN
                     HKTNYDNVQMSLF"
     gene            complement(977..1141)
                     /locus_tag="PLO59_00015"
     CDS             complement(977..1141)
                     /locus_tag="PLO59_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99501.1"
                     /translation="MPTGTHTTPPIAILYTPGQQTTQPLPTKSSMKNNIRSTSATILQ
                     NQCYRQVWVN"
     gene            complement(1289..2062)
                     /locus_tag="PLO59_00020"
     CDS             complement(1289..2062)
                     /locus_tag="PLO59_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HQU99502.1"
                     /translation="MSEGKKEILLGDCLELMKDIPNGSIDMILCDLPYGTTQNKWDVI
                     IPLDKLWEQYERIVKDNGMICLTSAEPFTSTLITSNKKLFKYDLIWDKKLSSGFLNAK
                     RMPLRRHEQILCFYKKLPIYNPEMVTRGKVRKKGITTETGKHTSNYGKFENSVVENNE
                     YYPTSIIEVSNANRKDKLHPTEKPVPLFEYLIKTYTNEGDVVLDNTAGSGTTAIACLN
                     TKRQFIVMEKEQKYYDIILKRVGDFNKNFEPQTLFGNEM"
     gene            complement(2110..2520)
                     /locus_tag="PLO59_00025"
     CDS             complement(2110..2520)
                     /locus_tag="PLO59_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99503.1"
                     /translation="MHITKQFQITLYEKEILITTPSNRKEAYQAALKFAEGKSMHLIE
                     RNGMRHDWIETSDKALNYYECCGVLILFGISPPSLDELYSLLASPTVVSSTVSKAFHI
                     TDGAISRFALQKCSNLAQMLLANLLIARVIRWHG"
     gene            complement(2593..2754)
                     /locus_tag="PLO59_00030"
     CDS             complement(2593..2754)
                     /locus_tag="PLO59_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99504.1"
                     /translation="MAKYSGKDEKINGKWYPVTVNADSLKEATKKLYVGQRKSYGTVQ
                     AVRGRFSKD"
     gene            complement(3046..3342)
                     /locus_tag="PLO59_00035"
     CDS             complement(3046..3342)
                     /locus_tag="PLO59_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99505.1"
                     /translation="MHTNETRQIQRINNLLQLYHHIGHQYTPHALHHPQHYVTHRSYL
                     FKKIMQAHALLKQMQHANRHPHNTTHCNPVPYAIYKHGIRPGNKQRSHYQLKAA"
     gene            complement(3357..6221)
                     /locus_tag="PLO59_00040"
     CDS             complement(3357..6221)
                     /locus_tag="PLO59_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99506.1"
                     /translation="MAYITPHDIYNKTNGGLDIILQYYPQAANVLNTPGKRFKMRGDE
                     RTASAAIKRGEDGVYIITDFGGDAKARNAISVVMDEEKINFRTAILTLAERLQIVPPA
                     MAPDALKAKFTKTPAPPNTPIGQYDVTTKDFTINEIKHLFTANVFDHNKPAPTPQTSV
                     ILETWEQTRLIELSALCSKYHLHSVAHYSFYKHATDTNGNSTGTIEHIKIESTDTYPI
                     FCFIETDFKKIYQPLALDKSRRFIYIGRPTKDFIFGLQQAQQHYNKLNADTAEADYDS
                     LPDEEKTASRAIKKLPEIIIATGGSDALNLAAIGYYPIWFNSESTIMHQGQYTTIARI
                     CDKVYNVPDIDDTGKKQAHKLALAYLDVHTVELPPTLADKTYFGKPCKDVRDFLKWHT
                     AREFADLLTIALPYKFWAETYKLNTKTGKPYLAEYEVQNTCLYHFLQKSGYHIYIDEN
                     EKESETFIHIDSTGAVRKVLPTEIKRYLFDFLKERRQPVKLRDVFYRSAQLSESSFKS
                     LEPRKVDFKCSTAGSQYLFFTNTTWHVTKDTITTHKPIDKPLNIWLDEIIPHSPKLLD
                     KPFTVTYQPEADTYDIAIHNPQCIFLQYLCRTSAMYWQKAATGQLLTPEESTEEKTHL
                     INKLYTFGYLLHRHKSPSRAWMPFIMENKISEDGVSEGGAGKSIFAKAVSHFCRTVFI
                     NGREKKINENKHLFENVTRHTKYVLLEDIDKYFDLGMLFEYITGIFSVNPKHSRSFTL
                     GYADSPKLAVTTNFGMRNEDPAKNRRVLYCSFSDYYHNGGPGLSHSTAFSPLDDFGKN
                     LFDDFTTDEWNHFFNTAAYALQLYLNFPKINPPLDNVQKRNMMETMGPEFRAWANSYF
                     AHTEAEADRKLDTFLVRKEVFDAYREVDKNSSPQLFFKKLTAWCRFKSYELNPKALHN
                     SDDRIVRRVAGKSTEMLYILTAANINPTHITDNNTQDTPF"
     gene            complement(6299..6682)
                     /locus_tag="PLO59_00045"
     CDS             complement(6299..6682)
                     /locus_tag="PLO59_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99507.1"
                     /translation="MCAQLLRYKRDINNKLFCQSWCRFQLHNVHTHVGQLFDVVLQEH
                     PESKPVQYGLHEVVAIHTYRFLEVPEHLCYIDVDMSLISWRTMYRRLLSISNTDNPLL
                     DCLILQGVAPFVVNKRTERNKALQV"
     gene            complement(6685..7017)
                     /locus_tag="PLO59_00050"
     CDS             complement(6685..7017)
                     /locus_tag="PLO59_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99508.1"
                     /translation="MITIEPAFNEQKQIEFSISKLTINDLCLLVQWAQLAFKINDASY
                     YDKQALQARHPTHERFLELQQMQDEMQFAETVRKQIDGAVNDAQHLIVKGVVAPRSPL
                     LIEPGKPL"
     gene            complement(7044..7268)
                     /locus_tag="PLO59_00055"
     CDS             complement(7044..7268)
                     /locus_tag="PLO59_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99509.1"
                     /translation="MLKLLRRLMGTDKPERVFTFHVRISVPVDVHKPTTIAGPVEVTA
                     RTKAVAQAKCITLCRRALKASTFTVTAYKN"
     gene            complement(7261..7539)
                     /locus_tag="PLO59_00060"
     CDS             complement(7261..7539)
                     /locus_tag="PLO59_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99510.1"
                     /translation="MEIKLIDANGTKLLQRNGQVLNCPFQQPLILPGAKSNVVSMQQQ
                     PEFQVVPTPCGTWCPKFSLHHYNDGETIYLQCGSETTKYNTAKNKLDA"
     gene            complement(7529..7801)
                     /locus_tag="PLO59_00065"
     CDS             complement(7529..7801)
                     /locus_tag="PLO59_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99511.1"
                     /translation="MKKNQHPPAPTFKQWYVSLNRAQQAGAKARIQNECALRGNTIFN
                     NWLYSYTGIAPIYQPIIEKIAGCKIDFTFTPSPSSYHAKKTVSNGN"
     gene            7912..8175
                     /locus_tag="PLO59_00070"
     CDS             7912..8175
                     /locus_tag="PLO59_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99512.1"
                     /translation="MDYDLLKEKLKEVYPSPSTDDVNWLQLSKHIQYSHTGIMRSFRN
                     KSLQVDVFETLCTLTNYPPGYFFKENKAGAYASIDTNGPICLA"
     gene            8262..8444
                     /locus_tag="PLO59_00075"
     CDS             8262..8444
                     /locus_tag="PLO59_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99513.1"
                     /translation="MHTMYSLRHTFISNLFQNGATSYELMSLTGHTTLAAFEKYIRSI
                     RDKTPIDVGAKLTISI"
     gene            complement(8506..9534)
                     /locus_tag="PLO59_00080"
     CDS             complement(8506..9534)
                     /locus_tag="PLO59_00080"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HQU99514.1"
                     /translation="MKKLLLFALLIVSQLTQAQTGIWCWAQWREAVPLSIYQNKNITG
                     VSLAFYWQDMEPQPGRYDFTRLNTELSKCVAHNKKAGISVAAGAKAPQWLKDKTPCLQ
                     FSEIRKDGDGKPYEVILPIPWDTTYLNAWQRFINALGAHIAARPTYNNIITHVALSGI
                     STTTCEMRLPQMEPTPQHPNVTNATVIWRNAGYDGFKILQSFNFCRRAWIKAFPQKKL
                     VIDMMLGNEFPLVDTVNINQGVFKACNATGNPGRYIFKHTSLTNNSRGTLITEIKNQG
                     FAAAWQTNQSMYGATPCAPACNDTSIKQALDIGIKFNVQFIEMQTLAIINYPQAVSYA
                     AIKMARMQ"
     gene            complement(9531..9791)
                     /locus_tag="PLO59_00085"
     CDS             complement(9531..9791)
                     /locus_tag="PLO59_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99515.1"
                     /translation="MIYQLLKVLYDGAPTETPDGLLCKMQILITAQGETNEEFAARKN
                     VTVIVPYNTPCTSIGDSITAIKNTVLQHCINYVNENFNTNQL"
     gene            complement(9797..10861)
                     /locus_tag="PLO59_00090"
     CDS             complement(9797..10861)
                     /locus_tag="PLO59_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99516.1"
                     /translation="MAKAVVRKRIVASGNTGGSGGGGGGESLAQTLAIGNVTGGNDIV
                     LSDAGANVQTQAIVSAYDVDSFIAFFNGIVSSLQLLGGATTSLNLSATGVYAYANAIE
                     FNNYAGGKLKMINADSSLYYHTDGSGYKFRITSTGLTMVGLIEALGNLVTGETLIYDG
                     RILFRNATNNKALYLKVGATVDDIEIVLPAAVPTVGALLKVASVTGSSPVTVTLDYES
                     ILEEVAETTYSGTIVWDGTAPTTLTNATYRWTRVGKMVNLRINIRYTNAGTSNTMVTL
                     TLPSGCPTPQDPSGFSSANDYGYIGSGSLGTGPGNMGGGNTRASLGPNATATGYVLTV
                     LAATALAAKVARISIDYYIP"
     gene            complement(10874..11209)
                     /locus_tag="PLO59_00095"
     CDS             complement(10874..11209)
                     /locus_tag="PLO59_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99517.1"
                     /translation="MKTNKAMQYKTLAYQLVTVITAPTTLTIPTDPTFKIREIILTAE
                     SVTGATNNALIRFTADGITDPDSNVGVPMYNGDTRSLESLADAQNLRMYCIEGNNQIV
                     HVEYLGLDQ"
     gene            complement(11196..11534)
                     /locus_tag="PLO59_00100"
     CDS             complement(11196..11534)
                     /locus_tag="PLO59_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99518.1"
                     /translation="MQNLYFLRTSQTEKITIDGDVVSNCSEIYIKNVGTAPFIVDGST
                     VAVGETYSETTCTPWAVNSKKYRIVVTVSVPKGTNVIEAEVKRVWYMPTKGEFPTSFN
                     PNLLMPNENK"
     gene            complement(11547..11981)
                     /locus_tag="PLO59_00105"
     CDS             complement(11547..11981)
                     /locus_tag="PLO59_00105"
                     /inference="COORDINATES: protein motif:HMM:NF039494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HQU99519.1"
                     /translation="MTARDFIQTALQAGFTMAHMPMVLSIGMWESKLNPQAVGDVNLI
                     NDTWGPSIGLMQIRTIKTPNANEHFRSTARIEALKNPFENLKAAYIISKGGTTWQPWT
                     AYSAGHYKTTYSTALQAVAQYAPTPTGIATAVMVGVILSTII"
     gene            complement(11985..12401)
                     /locus_tag="PLO59_00110"
     CDS             complement(11985..12401)
                     /locus_tag="PLO59_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99520.1"
                     /translation="MSAPVYSATEIIGKSLYGRVAIPIRRQAVDNMPIVYTAAPGKLI
                     GKVINFIAPRPGYNATLYWEFADANGVKYYTPHVEGYYDINNLQNQGAQTVQQQAEAE
                     AQANKSTSDKIIDTVKWVAVAAVVAVVGKSLIAKSN"
     gene            complement(12403..13158)
                     /locus_tag="PLO59_00115"
     CDS             complement(12403..13158)
                     /locus_tag="PLO59_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99521.1"
                     /translation="MLRGKENTLKWFDYNAASPIWQIAASNNPNQAIIGWGVNDTDED
                     DPENKKAMMDPGTSRGLLEEALNILEPGSYTIRLRKSVARGSKWLFTERIDTRDQTAN
                     TANNPAHIGHIGGYHGKTEAEIRADERAKVELEFKIKELERLLKEKNESGNEKISGVE
                     KLIPLLQPHMGHIIEGLFGKAPQVAVSGFDANDSQGTTVIETEQTRLENALQQLNEHL
                     EKRGKNIVAVLESLNHLANTQPATFDMALNFLK"
     gene            complement(13244..13708)
                     /locus_tag="PLO59_00120"
     CDS             complement(13244..13708)
                     /locus_tag="PLO59_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99522.1"
                     /translation="MKVAFPYANHESIEIPCTASAVRYNFLNNVADKLQNKYIVGIAA
                     HHVNTTTITPGNANVVNDTVFRKTYVTLSWADQERVSSWSLPTLQFAQTAAASAYVNG
                     FTPLNFLQINFYKSYVSVANAAGLVAGEAFLLSFWYVNTVPAAYKPMLEFNK"
     gene            complement(13809..14555)
                     /locus_tag="PLO59_00125"
     CDS             complement(13809..14555)
                     /locus_tag="PLO59_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99523.1"
                     /translation="MKNLVVKLLVCFAFISDLFGALGVSNGSLATGAPIGSNANMQRH
                     IFNQLRKEYPTHNIAPGYLRVEQTLSNSTGTYNFNVKVTGNELATERKLDQNDVFVCT
                     HIGFYLLNYVAATVGKQPLQSYANNTAFGATCTPELDGWYNGSMAIKINQTVSPEALP
                     LNLFRYVPTTQQSAATNLAEASAITTSSFLGGLIYFRGNQSINVTTTSPTWAGIVWGT
                     PTAGAVNLVFHPYGFLIKGAAAARILDGLR"
     gene            complement(14618..15019)
                     /locus_tag="PLO59_00130"
     CDS             complement(14618..15019)
                     /locus_tag="PLO59_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99524.1"
                     /translation="MKKVNFKKAATKIALTGGGAVASNFVGNQFLGAQKPIIRGAIKT
                     AIGAIVLPMLGKGDTFTSLGDGFIADGALEMAAAVIPGKVKIGYGGDAAVGESNFSID
                     EDFIQVSGYNDPAVGGYNDPAVGDVVPGMDL"
     gene            complement(15060..15284)
                     /locus_tag="PLO59_00135"
     CDS             complement(15060..15284)
                     /locus_tag="PLO59_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99525.1"
                     /translation="MATKKRTTKKKAAPAKRKKKAVSTGLRKFRTYVSKRIRSTAKKV
                     SELERKLKAAKKEKARKTKIAIAGYKKANR"
     gene            complement(15448..17253)
                     /locus_tag="PLO59_00140"
     CDS             complement(15448..17253)
                     /locus_tag="PLO59_00140"
                     /inference="COORDINATES: protein motif:HMM:NF016364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HQU99526.1"
                     /translation="MHQAAFLIPKPNNVSTTMHTHGDTDDIINVILFADAQAAKYTSS
                     FAKKLRGNTTLQTAANIWNFLKTEITYKDDGFKVQNIKSPAQFYWDKTGDCKSYSVFT
                     ASVLQNLNIPYCYRFASYSSNHTPTHVYIIIPATATTTEIIIDAVWIAFNNQKPYTHK
                     QDFKGMTKINYVAGPAPINTTTDTRLQLPPASQEWSDADIELAIILQRAQIMKSRIAG
                     IGTTGAARAEKFEDAIEAIKDIQKHLSDEERIGIILQDIDNKQYNTSTHINQLLSEEK
                     KYMQRIAIIKLRDAQQYARTRQAKLNAYEARIAAQQKRIATLTTEAATATTDQARTDI
                     NAEIAKRQQVLTTLLRIRDAVAVAGINGNVAVAGINGNVAVAGFLSDALKKASTAVKK
                     VTKAVVKVATAPARLIAKGALEISLPKAAPAFLYLFVTDPALVAKLPANAKTKRTKQE
                     RKANFIVNTIGMKRNHFMGIVRNGILKRYGKQPEQIIAELLKVAKVSGHSIGIAPAIL
                     AAGSMLNSKDKEGKNQLLGFLNELIGIISKMFGKSDTESMSAADLPDESDWTSLADAQ
                     AFELAGNITAQAPSPTATADELETQADGGRGFCNW"
     gene            complement(17257..17442)
                     /locus_tag="PLO59_00145"
     CDS             complement(17257..17442)
                     /locus_tag="PLO59_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99527.1"
                     /translation="MATKKTAKSIVNNTTGYDAKNRQVILQGNTVTKEATGYNHTFST
                     AAQAQTAFEFQCACSQN"
     gene            complement(17470..17727)
                     /locus_tag="PLO59_00150"
     CDS             complement(17470..17727)
                     /locus_tag="PLO59_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99528.1"
                     /translation="MKKILPLAAIAALAFFAMRKGAPTATAQRAAVAPKRILPPSALK
                     QAATAATPRKAVTNAEQVINTTIRAGQGKDTAEYIGMGNIY"
     gene            complement(17729..18766)
                     /locus_tag="PLO59_00155"
     CDS             complement(17729..18766)
                     /locus_tag="PLO59_00155"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HQU99529.1"
                     /translation="MKRTGIVLGAALLVGAFMAVGRNVINSAKQLNWQLSNYFKLSNI
                     KILNSDLSFAFKVTNTNTTPVAYSNVNFTGNVIYGGKSIADVNNTQSITLQPAETKII
                     NISVQLNHLKIGQSIISVIKDGNATALTLIGTVRYGVVSFPINQKLNLLKVAGISGRA
                     IINGIAGYFDLTEISTLDDLKKQYFKLAKTHHPDAGGTTADFQNLQAEYEILIRKILN
                     GTNLSSEEKNNEIVIDENLRQAIDTVIHLPGISIDLVGKWIWISGNTFPIKDFLKNAG
                     FVFFKKAGTAYWVFKGVESHSRGGTALQDIYNKYGKKNISPRPGNNYLGKAPANILRA
                     DYAKYANANYF"
     gene            complement(18766..18969)
                     /locus_tag="PLO59_00160"
     CDS             complement(18766..18969)
                     /locus_tag="PLO59_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99530.1"
                     /translation="MEAQKNIGPMQQFNSNEVAAFAGSLLTISWHGIYPDRILQTIVY
                     STIGAIIGISLNEAVKFLKSKIK"
     gene            complement(19055..19333)
                     /locus_tag="PLO59_00165"
     CDS             complement(19055..19333)
                     /locus_tag="PLO59_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99531.1"
                     /translation="MYNVFQYHLSLQPIKQHTNYFMKGIFVDVTDAMQVYGVSQRQAI
                     KKLNHIKLALNKPPGTRILVREFCKYEDIHEQDFLQAIKPHKPTTTTV"
     gene            complement(19971..20183)
                     /locus_tag="PLO59_00170"
     CDS             complement(19971..20183)
                     /locus_tag="PLO59_00170"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQU99532.1"
                     /translation="MIDTTIINLQRQNIGATLKQARLNAGLTYYALAKQAGLKLHQIQ
                     SIEAGTKAYTIDSYLRIAAVLGINAP"
     gene            complement(20331..21335)
                     /gene="dcm"
                     /locus_tag="PLO59_00175"
     CDS             complement(20331..21335)
                     /gene="dcm"
                     /locus_tag="PLO59_00175"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF012372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HQU99533.1"
                     /translation="MPSCLSHGSLFSGIGGFDLAAQWMGWNNIFQVEKDDWCRKVLAK
                     NFPKTERFSDIKDFTGYEYTNRINVISGGFPCQPFSVAGQRKGKDDDRYLWEEMLRVI
                     ATIKPTYVVGENVTGIIGLALDTVLSDLEAQNYTTETFIIPACGKNAWHRRDRVWIVA
                     YANSIGRQNEQKENGQPLCNGNGNDTIEKQGRGKQQCWTGKSSSVFPNTASSGTGMEE
                     YRSSGQERGASNEHQPTFLRQKNRKAGSKRFNASSGDVSSNTNDTGCEEQRQPITDGA
                     ELFAPKCSSWWETEPGVGRVVDRLPGRVDRLKGLGNSIVPQVAYEIFSAIGAVSWHGA
                     "
     gene            complement(21483..21779)
                     /locus_tag="PLO59_00180"
     CDS             complement(21483..21779)
                     /locus_tag="PLO59_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99534.1"
                     /translation="MKLKAKYKWNGEVVKVKPVFFSWHVGIEYKTLTGYSAASIFCNK
                     ESEYVFPLQTKIILFFVKIWYWMRYWLWMQIRYWQPNRIKRVLSKKEIGDDLPF"
     gene            complement(22010..22705)
                     /locus_tag="PLO59_00185"
     CDS             complement(22010..22705)
                     /locus_tag="PLO59_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99535.1"
                     /translation="MWSYYGSKSKMVGCYPPPKFDKVIEPFAGCAKYSLKYFEKQVTI
                     VDKYKVLIDTWKYLQQASENDILKLPKLKRGDSIKDHKYLSEVEQNFLGFLVCNGLES
                     PRMNVSSFEGVNVERDLKRIAKSLFKIRHWNIILGTYEDIENVEATYFIDPPYMFGGE
                     HYKESSKNINFENLAEWCKSRNGQVIVCENTKATWLPFLPIKTMSGSMYKTTEAIWSN
                     HKTNYDNVQMSLF"
     gene            complement(22719..22883)
                     /locus_tag="PLO59_00190"
     CDS             complement(22719..22883)
                     /locus_tag="PLO59_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99536.1"
                     /translation="MPTGTHTTPPIAILYTPGQQTTQPLPTKSSMKNNIRSTSATILQ
                     NQCYRQVWVN"
     gene            complement(23031..23756)
                     /locus_tag="PLO59_00195"
     CDS             complement(23031..23756)
                     /locus_tag="PLO59_00195"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HQU99537.1"
                     /translation="MKDIPNGSIDMILCDLPYGTTQNKWDVIIPLDKLWEQYERIVKD
                     NGMICLTSAEPFTSTLITSNKKLFKYDLIWDKKLSSGFLNAKRMPLRRHEQILCFYKK
                     LPIYNPEMVTRGKVRKKGITTETGKHTSNYGKFENSVVENNEYYPTSIIEVSNANRKD
                     KLHPTEKPVPLFEYLIKTYTNEGDVVLDNTAGSGTTAIACLNTKRQFIVMEKEQKYYD
                     IILKRVGDFNKNFEPQTLFGNEM"
BASE COUNT         5747 a         4647 c         5247 g         8150 t
ORIGIN      
        1 cctcaccatt ccatttgtat tttgctttta gtttcatatt taaaatttcg ttttaataat
       61 ccggcacgaa ccgcatacga aatgacatta acaaacacga cagaaaagaa gggcgaagtg
      121 ctaacagcgg ttttgcgtaa tggcgggtgc agtggtaaat tgaacatttg tgcttctatc
      181 atcattagtg ctaaattgaa agtgagtgcc ttgaaacgcc accgagcgta aagcccgaaa
      241 acgttatggg caagtgttag caccatacta aaataacgac atttgaacat tatcatagtt
      301 tgttttatgg tttgaccaaa tagcttccgt tgttttatac attgaacctg acattgtttt
      361 tataggtaaa aatggtagcc aagttgcttt tgtgttttcg caaactatta cttgtccgtt
      421 tctactttta caccattctg ccaagttttc aaagttaata tttttactac tttctttgta
      481 gtgttcgcca ccaaacatat acggtgggtc tataaagtaa gtagcttcta cattttcaat
      541 atcttcatac gttcctaata ttatattcca gtgcctaatt ttaaataaag attttgcaat
      601 ccgctttaaa tctcgttcta cattcacacc ttcaaagcta cttacattca ttcgtggact
      661 ttctaatccg ttgcaaacta aaaaacctaa aaagttctgc tctacttctg ataagtattt
      721 atggtcttta atgctatcac cacgttttaa ttttggcaat tttaaaatat cattttcact
      781 tgcttgttgt aaatacttcc aagtatcaat aagcactttg tatttatcta caatagttac
      841 ttgcttttca aaatacttca atgaatattt tgcacaacca gcaaaaggtt caataacctt
      901 atcaaatttt ggcggtggat aacaaccaac cattttactt ttacttccgt agtatgacca
      961 catttttatt tcgtttttaa ttaacccaca cctgcctgta acattggttt tgcaatatgg
     1021 tggcagaagt gcttcgtatg ttgtttttca tgctgctttt agttggtagt ggctgcgttg
     1081 tttgttgccc gggcgtatac aggattgcaa tgggtggtgt tgtgtgggtg ccggttggca
     1141 tgttgcattt gttttagtag tgcatgggct tgcattattt ttttaaatag gtagctgcgg
     1201 tgggttacat agtgttacct gcaagcgggc gaacctttgc tactgtatag cccgccttcg
     1261 gttagctgca aaacgttata cgcaagcact acatttcgtt tccaaagaga gtttgcggtt
     1321 caaaattttt attaaaatct cccacccgct ttaaaataat atcgtaatac ttttgttctt
     1381 tttccattac tataaattgc cttttagtat tcaaacaagc tattgcagtc gttccgctcc
     1441 ctgctgtgtt gtctaatact acgtctcctt catttgtgta ggtttttatt aagtattcaa
     1501 ataaaggcac tggcttttcc gttgggtgca gtttgtcttt tctatttgcg ttgctaactt
     1561 cgattattga agttgggtag tattcattat tttctactac gctattctca aactttccat
     1621 aattacttgt gtgcttccca gtttcggttg ttatcccttt ctttcttact ttccctcttg
     1681 taaccatttc agggttgtat atcggtagtt ttttgtaaaa gcaaagtatt tgttcgtgcc
     1741 ttctcaacgg catacgttta gcatttaaaa atccacttga aagtttttta tcccaaatca
     1801 agtcgtactt aaataacttt ttatttgaag ttatcagtgt gcttgtaaat ggttcagcac
     1861 ttgttaaaca aatcattcca ttatctttta caatcctttc gtactgttcc caaagtttat
     1921 caagtggaat aattacatcc catttatttt gtgtcgttcc atatggtaaa tcacaaagta
     1981 tcatatcaat actcccgttt ggtatatcct tcataagttc caaacaatct cctaataata
     2041 tttctttttt gccctcgctc attttaataa aaattttgtt tcgtgtttca atttaagttt
     2101 atcgtttaat caaccgtgcc agcgtataac acgtgctata agcaagtttg ccaataacat
     2161 ttgtgctaaa tttgaacatt tctgcaaggc aaacctgctt atagcaccat ccgttatatg
     2221 aaatgccttg ctgaccgtgc tacttacaac cgttggactg gctaacagac tgtatagttc
     2281 atctaaactt ggcggactaa ttccaaacag aattaggaca ccacagcatt cataatagtt
     2341 gagagcttta tcgcttgttt caatccaatc atgacgcatt ccatttcttt caatgaggtg
     2401 catacttttt ccttctgcaa attttagtgc agcttgataa gcctcttttc tgtttgacgg
     2461 tgttgtaatt aaaatctctt tttcgtaaag agtaatttga aattgttttg ttatgtgcaa
     2521 ggcgggggca gtgcttcgtt tgaagttctg tgatgaaaat tttagaaaca aaacccccac
     2581 cgcacataaa aattaatcct ttgaaaagcg acctcttaca gcttgaacag ttccgtaact
     2641 tttacgctgt cctacataaa gttttttggt tgcttctttc aaagaatctg cattaacggt
     2701 aactggatac cattttccgt taatcttttc gtctttacct gaatatttag ccattgtatt
     2761 ttgatttaat tgtttaattg aataatgaat ttgctcccct tcgttttgtt tctaaaattt
     2821 tctttcgtgc ttcgtatcaa atgtggtggt gagaagccgc ccagcacata acagcacatt
     2881 tgcgttatgg cggttgtcgt ggtttaattg agtttggtat ttctttcata attccgttca
     2941 aatttaatag ttaagtgctt ctaatccgcc acaaacgcaa atctgcaacc cgttgttgct
     3001 gacttttcca tatttattta gtttaaatta attgtttgtt gtttttcatg ctgcttttag
     3061 ttggtagtgg ctgcgttgtt tgttgcccgg gcgtatgccg tgtttgtata tagcataggg
     3121 tacaggattg caatgggtgg tgttgtgtgg gtgccggttg gcatgttgca tttgttttag
     3181 tagtgcatgg gcttgcatta tttttttaaa taggtagctg cggtgggtta catagtgttg
     3241 tgggtggtgt agtgcatggg gtgtgtattg gtggcctatg tggtggtaaa gttgcagtag
     3301 gttgtttatg cgttgtattt ggcgggtttc gttggtgtgc atagtgtata agggttttaa
     3361 aatggtgtgt cttgtgtgtt gttatcggtt atgtgtgtgg ggttaatgtt ggcggctgtt
     3421 agtatgtaaa gcatttcggt gcttttgccg gcaacacgcc taactattct atcatcacta
     3481 ttgtgtaggg ccttggggtt tagttcgtag cttttgaagc ggcaccatgc cgtaagtttt
     3541 ttaaaaaaca gttgggggct gctgtttttg tccacttcgc gataagcgtc aaaaacttct
     3601 ttgcgtacta aaaaagtatc cagtttgcgg tcggcctcgg cctcggtatg ggcaaaatag
     3661 ctattggccc acgccctaaa ttcgggcccc attgtttcca tcatgttacg cttttgcaca
     3721 ttgtcaagcg gtgggtttat ttttggaaaa tttaagtaca gttgcagcgc gtatgctgct
     3781 gtattaaaaa agtggttcca ttcatcggtg gtaaaatcat caaacaaatt ttttccaaaa
     3841 tcatcaagcg ggctaaatgc tgtagagtgc gaaaggcccg gcccgccatt gtggtaataa
     3901 tcgctaaagc tgcaatacag tacgcgcctg tttttggcgg ggtcttcgtt gcgcatgcca
     3961 aagtttgtag taacggccag tttggggctg tcggcatagc caagggtaaa gctccggctg
     4021 tgctttgggt ttacgctaaa tataccggtt atgtattcga acaacatacc aaggtcaaag
     4081 tacttgtcta tatcttcaag caatacatac ttagtgtggc gggttacatt ttcaaataaa
     4141 tgcttgtttt cattaatttt tttttcgcgg ccgttaataa atacggtacg gcaaaaatgg
     4201 cttacggctt ttgcaaaaat agatttgccc gccccgccct cgcttacgcc atcttcgcta
     4261 attttgtttt ccattataaa cggcatccat gcgcggcttg gcgacttgtg gcggtgcaat
     4321 aagtagccaa atgtatatag cttgtttatg aggtgtgttt tttcctcggt gctttcctcg
     4381 ggggttaaca gttggccggt ggctgccttt tgccaataca tggcactggt acggcatagg
     4441 tattgcaaaa aaatacattg cgggttgtgt atggctatat cgtaggtgtc ggcctcgggt
     4501 tggtatgtta ccgtaaatgg tttgtctaac agctttggcg agtgtggtat aatttcgtca
     4561 agccaaatgt ttaaaggttt atcaatgggc ttgtgtgtgg taatggtatc ttttgtaacg
     4621 tgccaagtag tgtttgtaaa aaataaatat tggctgccgg ctgtgctaca tttaaaatca
     4681 accttgcgtg gctcgaggct tttaaaactg ctttcgctaa gctgtgcgct acgataaaaa
     4741 acatcgcgca atttaaccgg ctgcctacgc tcttttaaaa aatcaaatag gtagcgtttt
     4801 atttcggtgg gcagcacttt gcgcacggcc ccggtgctgt ctatgtgtat aaatgtttcg
     4861 gattcttttt cgttttcgtc aatataaatg tgatagccgc ttttttgcaa aaagtggtat
     4921 aagcaagtgt tttgtacttc gtattcggca aggtatggtt tgccggtttt ggtgtttagc
     4981 ttataggttt cggcccaaaa tttatagggt aatgcaatgg ttaaaaggtc ggcaaattcg
     5041 cgggcggtgt gccattttaa aaagtcgcgc acatctttgc atggcttgcc aaaatatgtt
     5101 ttgtcggcca atgttggggg cagttcaacg gtgtgcacat cgaggtaagc aagggccagt
     5161 ttgtgggctt gtttttttcc ggtgtcgtca atatcgggca cgttgtaaac cttatcacaa
     5221 atgcgcgcaa tggtggtgta ttggccttgg tgcatgatgg tgctttcgct attaaaccaa
     5281 atggggtagt agcctatggc cgcaaggttt agcgcatcgc tgccaccggt ggcaattata
     5341 atttcgggca atttttttat agcgcggctt gctgtttttt cttcatcggg caagctatca
     5401 tagtcggcct cagccgtgtc ggcattaagt ttattgtaat gctgttgggc ttgctgtagg
     5461 ccaaaaataa aatctttggt ggggcggcct atgtatataa acctacggct tttgtcgagg
     5521 gccaagggtt ggtatatttt tttaaaatcg gtttcgataa aacaaaaaat agggtaagta
     5581 tcagtactct caattttaat gtgttcaatg gtgccggtgc tgttgccgtt ggtatcggtg
     5641 gcatgcttgt aaaagctgta atgtgccaca ctgtgtaaat ggtatttgct acaaagcgcg
     5701 cttagttcaa taaggcgtgt ttgctcccac gtttcaagta tgacgcttgt ttgtggtgtg
     5761 ggtgctggct tgttgtggtc aaacacgttg gcggtaaata ggtgttttat ttcgttaatg
     5821 gtaaaatctt tagtggttac atcgtactgg ccaataggtg tattgggggg ggcgggggtt
     5881 ttggtaaatt ttgcttttag cgcatcgggc gccattgcgg gcggcactat ttgcaatcgc
     5941 tcggcaagcg ttaaaatggc ggtgcgaaaa tttatttttt cctcgtccat cactacgcta
     6001 atagcgttgc gggctttggc atcgcctcca aaatcggtaa taatgtaaac accatcttcg
     6061 ccccgcttaa tggctgcgct tgctgtacgc tcatcgcctc gcattttaaa ccgtttgccg
     6121 ggcgtgttta gcacattggc ggcttgcgga taatattgta aaataatgtc taagccgcca
     6181 tttgttttat tgtaaatatc gtgtggggta atgtatgcca taggtgttta taggggtgta
     6241 gggtgcaagg cttttaattt aaaaagcggc tgcgttgtgt gtgtgtaaat gtgttgtgct
     6301 atacctgtag cgccttgtta cgctcggtgc gcttgtttac tacaaatggc gccacgcctt
     6361 gcagtattag gcaatcaagc aaagggttat cggtgtttga tatgctaagt aggcgcctgt
     6421 acatggtacg ccagcttatt aaactcatat ctacatcaat gtaacaaagg tgctcgggca
     6481 cttctaaaaa cctgtaggta tgtatagcaa ccacctcatg taagccgtat tgcaccggct
     6541 tgctttcggg gtgttcctgt agcactacat caaataattg gcctacatgt gtgtgtacat
     6601 tgtgcagttg gaaacggcac cagctttggc aaaacagctt attgttaatg tcgcgcttgt
     6661 agcgcaatag ttgagcacac atgtttataa tggtttgcca ggttcaatta gtagtgggct
     6721 tcttggtgct actactccct ttacaattaa atgctgtgca tcattaacgg caccatctat
     6781 ttgtttgcgc acggtttcgg caaattgcat ttcatcttgc atttgttgca gttctaaaaa
     6841 ccgttcgtga gtcgggtgcc gcgcttgcag tgcttgttta tcgtagtagc tggcatcatt
     6901 aattttaaac gccaattgtg cccactgcac caataaacat aaatcgttaa tggttagctt
     6961 gcttatgcta aattcaattt gtttttgctc gttaaaagcg ggttcaatag ttatcatagt
     7021 gcgatatgtt aaagggtttg cgtttagttt ttgtaagccg ttacggtaaa tgtcgaagcc
     7081 tttaaagccc tccggcaaag ggttatgcac ttagcttgtg ccacggcttt tgttcgggcg
     7141 gttacttcta ccgggccggc aatggttgta ggcttgtgta catctaccgg cacactaatg
     7201 cgcacatgaa atgtaaatac gcgctcgggt ttatcggtgc ccattaggcg ccttagtagt
     7261 ttaagcatct aatttgtttt tagcggtgtt atacttagtg gtttcgctgc cacattgtag
     7321 ataaatggtt tcgccatcgt tataatggtg taggctaaat ttggggcacc atgtgccgca
     7381 aggtgtgggc actacttgaa attcgggctg ctgctgcatg cttacaacgt tgctttttgc
     7441 accgggcaaa attaacggct gctgaaatgg gcagttaagc acttggccat tgcgttgtaa
     7501 tagcttggtg ccgtttgcgt caattaattt aatttccatt gcttacggtt ttttttgcgt
     7561 ggtagctcga tgggcttggc gtaaacgtga aatctatttt gcagcccgct attttttcga
     7621 taataggctg ataaataggc gctattcctg tgtaactgta aagccagtta ttaaatattg
     7681 tgttgccacg tagggcgcac tcattttgta tgcgcgcctt ggccccggct tgctgcgcac
     7741 ggtttaggct tacataccat tgtttaaagg taggggcggg cgggtgttgg tttttcttca
     7801 tgcaatactt ataaaggttt tctaaattta taacgacaaa tgaaacattt gtttaaatac
     7861 ggtgtacaat aatctacaca taagttttca acacacttat taacttgtga tatggattat
     7921 gatttattaa aagaaaaatt aaaagaggtg tacccaagcc caagcactga cgatgtaaac
     7981 tggctacagt tgagcaagca catacagtac tcgcacactg gtattatgcg aagttttaga
     8041 aacaaaagtt tgcaagtaga tgtatttgaa acattatgta cactaactaa ttacccgccc
     8101 gggtattttt ttaaagagaa taaagcgggg gcctatgctt caattgatac aaacggccca
     8161 atatgtttag cctaagcggt aggccggggc cggtgccttt gggaaaaaat tatatgacta
     8221 aacaatttaa ggttttaaaa aaccagttgc aacttagccc tatgcacact atgtacagct
     8281 tgcgccacac gtttataagc aacctttttc aaaacggtgc tacatcatac gaattaatga
     8341 gtttaaccgg acatactaca ttggctgcat ttgaaaaata tatacgtagc attagagata
     8401 aaacgccaat tgatgtaggc gctaaactta ctataagtat ataaaaaaac cctacgcgct
     8461 ttggtagggc ttttaagaaa aacaatttaa aacacatcct atttattatt gcatacgcgc
     8521 cattttaatt gctgcatagc taacagcttg cgggtaatta ataattgcaa gcgtttgcat
     8581 ttctataaat tgcacgttaa actttatacc aatatctaag gcttgtttta tgcttgtatc
     8641 attgcaagct ggtgcgcaag gtgtagcacc gtacatgctt tggttggttt gccaagcggc
     8701 cgcaaagcct tggtttttta tttcggttat taaagtacct cggctgttat tggttaggct
     8761 ggtgtgttta aatatatagc gacccgggtt gcctgtagca ttgcatgctt taaatacacc
     8821 ttggtttata ttaacggtat ctactaaagg aaattcattt cctaacatca tatcaattac
     8881 taattttttt tgcggaaatg ctttaatcca tgcgcgccta caaaaattaa aactttgtaa
     8941 aattttaaaa ccatcataac cggcatttcg ccaaataact gtggcattag ttacattagg
     9001 gtgttggggc gtaggctcca tttgtggcaa tcgcatttcg caagtagttg tactaatgcc
     9061 actaagtgca acgtgtgtaa taatgttgtt atatgtaggg cgggcggcaa tgtgtgcgcc
     9121 aagtgcattt ataaagcgtt gccaagcgtt taaataggtg gtgtcccaag gtattggcaa
     9181 tattacttcg tatggcttac catcgccatc tttacgtatt tcgctaaact gcaaacaagg
     9241 tgttttgtct tttagccact gtggcgcttt ggccccggct gccacactaa tgccagcttt
     9301 tttgttgtgg gccacacatt tgctaagttc ggtattaagg cgcgtaaaat catagcggcc
     9361 cggttgtggt tccatatctt gccaataaaa tgcaaggctt acaccggtaa tgtttttatt
     9421 ttgataaatg gatagtggca cggcctcgcg ccactgtgcc caacaccaaa tgccggtttg
     9481 cgcttgtgtt aattggctta caattaatag ggcgaataat agtagttttt tcataattgg
     9541 tttgtattaa aattttcgtt cacatagtta atgcagtgct gaagcacggt gtttttaata
     9601 gccgttatac tgtcgcctat gctggtgcaa ggggtgttgt atggcactat tactgttaca
     9661 ttttttcggg ctgcaaattc ttcgtttgtt tcgccttgcg cggtaattaa aatttgcatt
     9721 ttgcacaaaa gcccatcggg cgtttcggtg ggtgctccat cataaagcac ttttaataat
     9781 tggtatatca ttttttttat ggtatataat aatctatact aatgcgcgct actttggctg
     9841 ccagtgctgt agcggctaat acagtaagca catagccggt tgctgtggca ttggggccaa
     9901 gcgatgcgcg cgtattgcca ccgcccatat tgccggggcc tgtgcctaaa ctgccactac
     9961 ctatatagcc gtaatcattt gcactgctaa agccgcttgg gtcttgcggt gtggggcaac
    10021 cacttggtaa tgttagcgtt accatagtat ttgacgtgcc ggcattggtg tagcgtatgt
    10081 ttatacgtaa gttaaccatt ttgccaacgc gcgtccatct gtaagtggcg tttgttaatg
    10141 tggttggcgc tgtgccatcc catacaatgg tgccactgta ggttgtttcg gctacttctt
    10201 ctaaaatact ttcataatca agtgtaacgg ttaccgggct gctgcctgtt actgatgcta
    10261 cttttaacaa tgcgcctact gtgggtacgg ctgctggcaa tactatttct atatcatcaa
    10321 cagtggcacc tacttttaaa taaagtgctt tattgtttgt ggcatttcta aataatatac
    10381 ggccatcata aattaatgtt tcgcctgtta ctaaattgcc taatgcttct ataagcccta
    10441 ccattgttaa gcctgtgctt gttatcctaa atttatatcc gctaccatcg gtatggtaat
    10501 ataaactgct atcggcatta atcattttta gtttaccgcc tgcatagttg ttaaactcta
    10561 tagcattggc ataggcataa acaccggttg cactaaggtt taatgatgta gtggcaccgc
    10621 ctaataattg taatgatgaa actatgccat taaaaaaggc aataaaacta tctacatcat
    10681 acgcgctaac tatggcttgg gtttgcacgt tggcgcctgc atcgctaagt acaatatcat
    10741 tgccaccggt aacattgcca atggctaagg tttgtgcaag gctttcgcca ccgccaccgc
    10801 caccgctacc gccagtattg ccactggcta caatgcgctt tcttactact gcttttgcca
    10861 tgtgtttaaa aaattattgg tcaagcccca agtactctac gtgaactatt tggttattgc
    10921 cctctatgca gtacattcgt aggttttgcg catcggcaag gctttctaaa ctccgcgtgt
    10981 cgccattata cataggcaca cctacattgc tatcagggtc ggttatgcca tcggccgtaa
    11041 atcgtataag tgcattattg gttgcaccgg ttacgctttc ggccgttaaa ataatttcgc
    11101 gtattttaaa tgtagggtcg gtgggtatgg ttagcgtggt gggcgctgtg ataacggtaa
    11161 caagttggta agctaatgtt ttgtattgca tggctttatt tgttttcatt aggcattaaa
    11221 aggtttgggt taaagctggt agggaattcg cccttggtag gcatgtacca tacgcgcttt
    11281 acttcggcct caattacatt ggtgcctttt ggtacgctaa cagttactac tattctatat
    11341 tttttgctat taacggccca cggtgtacaa gtggtttcgc tatacgtttc gcccacggct
    11401 acagtgctgc catcaactat aaatggggct gtacctacgt tttttatata aatttcgctg
    11461 caattgctaa ctacatcgcc atcaatggtt attttttcgg tttgcgaggt gcgcaaaaaa
    11521 taaaggtttt gcatgtatgt ttttttttaa ataatagtgg ataatataac gcctaccata
    11581 acggccgtgg caatgccggt aggtgttggc gcgtattgtg ccactgcttg caaagctgtg
    11641 ctataggtgg ttttgtagtg gccggcactg taggccgtcc atggttgcca tgtggtgccg
    11701 cccttgctaa tgatgtaagc ggcttttaaa ttttcgaaag ggttttttag tgcttcaatt
    11761 cgggcggtgc ttctaaaatg ctcgttggcg tttggcgttt taatggttct aatttgcatt
    11821 aagccaatgc ttggccccca tgtatcgttt attaaattta catcgcccac ggcttgcggg
    11881 tttagtttgc tttcccacat gccaatactt aacaccattg gcatgtgtgc catagtaaat
    11941 ccagcttgta gggcggtttg tataaagtcg cgggctgtca ttttttaatt actttttgca
    12001 attaatgatt tgcctaccac ggccaccacg gccgccactg ctacccattt aacagtatca
    12061 ataattttgt cgctggtgct tttgttggct tgcgcctcgg cctcggcttg ttgctgcacg
    12121 gtttgcgcgc cttggttttg taaattattt atatcataat aaccctctac atgtggcgtg
    12181 taatacttta caccgtttgc atcggcaaac tcccaataca aggtggcgtt ataaccaggg
    12241 cggggcgcaa taaaattaat aaccttgcct attagcttgc cgggtgctgc tgtgtacaca
    12301 ataggcatat tatcaacggc ttggcgccta atgggtattg ctacacggcc gtaaaggctt
    12361 ttgccaatta tttcggtggc actatatacc ggtgcactca tattatttta aaaaatttaa
    12421 agccatatca aacgttgccg gttgcgtgtt ggctaagtgg ttaaggctct ctaaaacggc
    12481 aactatattt tttccgcgtt tttccaagtg ctcgttaagt tgttgcagtg cattttcgag
    12541 gcgcgtttgt tcggtctcta ttactgtagt gccttggcta tcgttggcat caaagccact
    12601 aacggctact tgtggcgctt tgccaaatag gccttctata atgtggccca tgtggggttg
    12661 tagtagtggt attagttttt caacgccact tattttttcg ttgccgcttt cgtttttttc
    12721 ttttagtagg cgctccagct ctttaatttt aaattcaagt tcaactttgg cgcgctcgtc
    12781 tgctcttatt tcggcttcgg ttttgccatg atagccgcct atgtggccaa tatgtgcggg
    12841 gttgttggcg gtgttggcgg tttggtcgcg ggtatcaatg cgctcggtaa acagccactt
    12901 gctgccacgc gccacgcttt tgcgtagcct aatggtatag ctgcccggtt caagtatatt
    12961 aagtgcctct tcaagtaagc cgcgactggt gccggggtcc atcatggcct ttttgttttc
    13021 ggggtcgtcc tcgtcagtat catttacgcc ccaccctata atagcttggt tagggttatt
    13081 gcttgcagca atttgccaaa taggcgaagc tgcgttgtag tcaaaccatt taagtgtgtt
    13141 ttctttgcct cgtagcatgt gatgtgtttt aaagggttat tttaaaaacc cacacgcttt
    13201 tttaaatggg cgtgtgggca aaacttaaac tatatgaaac ctactattta ttaaattcaa
    13261 gcatgggctt gtaagcggcc ggcacggtgt ttacatacca aaagctaagc aaaaaggctt
    13321 cgcccgctac taagccggct gcattggcca cacttacata agacttataa aagtttattt
    13381 gcaaaaagtt aagcggtgta aaaccgttaa cgtatgcgct tgcggctgct gtttgtgcaa
    13441 actgcaaggt gggcaggctc cacgaactta cacgctcttg gtcggcccat gatagtgtaa
    13501 cgtatgtttt gcgaaacacg gtatcattaa ccacgtttgc attgcccggg gttatggtgg
    13561 tggtgttaac gtggtgggcg gctatgccta caatgtattt attttgtagc ttatcggcca
    13621 cgttgtttaa aaagttatac ctaacagcac tggccgtaca tggtatttct atgctttcgt
    13681 ggtttgcgta tggaaatgca actttcattt gtatttgtgt tatgtgtgtg tgtgggtttt
    13741 aaataacaat gggcgcaatg ctttgcgcct tgcgcccatt gtaaggctta atgattgtgt
    13801 gtgtgtgctt agcgtaggcc gtcaagtatg cgggctgctg ctgcaccttt tattaaaaag
    13861 ccataggggt gaaatactaa gttaacggca cctgctgtag gggttcccca tacgataccc
    13921 gcccacgttg gcgaggtggt agtaacgtta atactttggt tgcctctaaa gtatatgagg
    13981 ccgccaagga aactgcttgt tgtgatggcg cttgcctcgg ccaaattggt ggccgcgctt
    14041 tgttgcgtag tgggcacgta gcgaaatagg ttaagcggta atgcctcggg gcttactgtt
    14101 tggtttattt taatggccat gctgccattg taccagccat caagctcggg cgtacatgtg
    14161 gcgccaaatg ctgtgttgtt ggcatacgat tgcaaaggct gcttacctac agtggctgct
    14221 acgtagttta ataggtaaaa accaatgtgg gtgcacacaa atacatcgtt ttggtctaac
    14281 ttacgctcag ttgcaagttc attgccggta acttttacgt taaaattgta cgttccggtg
    14341 ctgttgctta atgtttgctc tacacgtagg taacccgggg ctatgttgtg cgttgggtac
    14401 tctttgcgca actggttaaa tatgtggcgt tgcatgttgg cgttgctgcc aataggggca
    14461 ccggttgcaa ggctgccatt agaaacgcca agggcgccaa ataaatcgct aataaaagcg
    14521 aaacaaacaa gaagttttac tactaaattt ttcattattt ttgaaattaa aagggttttt
    14581 tttgaattga tattaattgc gtgtgagttg tgtgtggtta taaatccatg ccgggtacta
    14641 catcgcctac ggcagggtcg ttatacccgc ctacggcagg gtcgttatag ccgcttactt
    14701 ggataaaatc ttcgtcaata ctaaaattgc tttcgcctac ggctgcatcg ccaccatagc
    14761 ctattttaac tttgcccggt ataacggctg ctgccatttc taaagcacca tcggcaataa
    14821 aaccatcgcc aaggcttgta aatgtgtcgc ctttgccaag cattggtaat acaatagcac
    14881 caatagctgt tttaattgcg cctctaatga ttggcttttg agcgcctaaa aactgattgc
    14941 ctacaaagtt tgaggcaacg gccccgccac ctgtaagggc aatttttgta gcggcttttt
    15001 taaaatttac ttttttcatt ttctataaaa ataacgtggt tggtttgggt tgtgtatgct
    15061 tagcggttgg cttttttgta gcccgcaata gctattttgg ttttgcgcgc tttttctttt
    15121 ttggcggctt ttaatttgcg ctccaattcg cttacttttt tagcggtgct acgtatgcgc
    15181 ttgcttacgt atgttctaaa tttgcgcaaa ccggtgctta ctgctttttt tttgcgtttg
    15241 gctggtgctg cctttttttt ggtggtacgt tttttagttg ccattttttt tgtttgaggt
    15301 tttaataaaa aagcattgcg cttacgttat gggcacaatg ctttttaaat cgcctatgaa
    15361 ttaaccactg caattatatg cgcggtgttg tgtgtgtgtg tgttgttggt gcatgtaatt
    15421 gcatacatgt gcatgtaatt gcgttgttta ccagttgcaa aagccacggc cgccatcggc
    15481 ttgcgtttct aattcatcgg ctgtggctgt ggggcttggg gcttgcgctg taatattgcc
    15541 tgcaagttca aaggcttgcg catcggcaag gcttgtccaa tcgctttcat cgggcaagtc
    15601 ggccgcgctc atgctttcgg tatctgattt gccaaacatt ttagaaataa tacctattag
    15661 ttcgtttaaa aaaccaagca attgattttt gccctcttta tcttttgaat taagcatgct
    15721 gccggctgca agtattgctg gtgcaatgcc aatgctatgg ccgctaacct ttgctacttt
    15781 taaaagttcg gctattattt gctcgggctg cttgccgtat cgctttaaaa tgccattgcg
    15841 cactataccc atgaaatggt ttcgtttcat gccaatggta tttacaataa aatttgcttt
    15901 gcgctcttgc ttggtgcgct ttgttttggc gttggctggc aattttgcaa ccagggcggg
    15961 gtcggtaacg aataaatata aaaatgccgg tgctgctttt ggcaacgaaa tttctaaagc
    16021 gccttttgca attaaacgcg ctggggctgt ggctactttt accacggcct tggttacttt
    16081 tttaacggcc gtgcttgctt tttttagtgc atcgcttaaa aaaccggcaa cggcaacatt
    16141 accgtttata ccggcaacgg caacattacc gtttataccg gcaacggcaa cggcatcgcg
    16201 tatgcgcaac aatgtagtaa gtacttgctg gcgttttgca atttcggcat taatatcggt
    16261 gcgggcttgg tcggtggtgg cggtggctgc ctcggtggta agtgtggcaa tacgtttttg
    16321 ctgtgctgca atgcgcgctt cgtaggcgtt tagtttggct tggcgtgtgc gcgcatactg
    16381 ttgcgcatcg cgtagcttta taatggctat gcgttgcatg tatttttttt cttcgctaag
    16441 caattggtta atgtgtgtgc tggtgttgta ttgcttgttg tcaatatctt gcaaaataat
    16501 gccaatgcgc tcctcatcac ttaaatgttt ttgaatatct tttattgctt cgatggcatc
    16561 ttcaaatttt tcggctcggg cggctccggt ggtgccaatg ccggcaatgc ggcttttcat
    16621 tatttgtgcg cgttgcaaaa taatggccag ttcaatatct gcatcgctcc actcttgcga
    16681 ggcgggcggt agttgcaaac gtgtatcggt ggtggtattg atgggcgcgg ggccggctac
    16741 gtagttgatt tttgtcatgc ctttaaagtc ttgtttgtgt gtgtacggtt tttgattatt
    16801 aaaagcaatc caaacggcat ctataattat ttcggttgtg gttgctgtgg ctggtattat
    16861 aatataaacg tgtgtgggcg tgtggttgct gctgtagctt gcaaaacggt aacagtatgg
    16921 tatgtttaag ttttgcaaaa ctgatgcagt aaaaacgcta tagcttttgc agtcgcctgt
    16981 tttgtcccaa taaaattggg cggggctttt tatattttgc actttaaaac catcgtcttt
    17041 ataagtaatt tcggttttta agaaattcca aatattggcg gccgtttgca atgtggtatt
    17101 gccgcgcaat ttttttgcaa aactgctggt atatttagcg gcttgcgcat cggcaaaaag
    17161 tattacatta ataatgtcgt cagtatcgcc atgtgtgtgc atggtggtgc ttacattatt
    17221 aggctttgga ataaggaagg ccgcttggtg catggcttag ttttgactgc aagcgcattg
    17281 aaactcaaat gcagtttgtg cctgagctgc tgtgctaaag gtgtgattat aaccggtggc
    17341 ctctttagtt acagtgttgc cttgtaaaat tacttggcgg ttttttgcat cgtagccggt
    17401 ggtattattt acaattgact tggctgtttt ttttgttgcc attatatata agtgttttaa
    17461 aaataaatat taataaatgt tgcccatgcc tatgtattcg gccgtgtctt tgccttggcc
    17521 cgcgcgtatg gtggtgttta ttacttgctc ggcattggta acggctttgc gtggtgtggc
    17581 tgctgtggcg gcttgtttca atgcgcttgg tggtagtatg cgcttgggtg ccacggctgc
    17641 tcgttgtgct gttgcagtgg gtgcgccttt acgcattgca aaaaatgcaa gggcggcaat
    17701 agctgccagt ggtagtattt ttttcatgtt aaaaataatt tgcgtttgca tatttagcat
    17761 agtcggcccg caagatgttg gccggtgctt tgcctaagta attattgccc gggcgtgggc
    17821 ttatattttt tttgccgtat ttattataaa tgtcttgcaa ggctgtgccg ccacggctgt
    17881 ggctttcaac acctttaaac acccagtaag cggtgcccgc ttttttaaaa aaaacaaagc
    17941 cggcattttt taaaaaatct tttataggaa atgtgttgcc gctaatccat atccattttc
    18001 ctaccaagtc aatactaata ccgggcaagt gtataacggt gtcaatggct tggcgtaggt
    18061 tttcatcaat tacaatttcg ttgttttttt cttcgctact taaattggtg ccgtttaaaa
    18121 ttttgcgaat taaaatttca tattcagctt gcaaattttg aaagtcggcc gtggtgccgc
    18181 cagcatcggg gtggtgcgtt tttgcaagtt taaaatattg cttttttaaa tcgtcaaggg
    18241 tgcttatttc ggttaggtca aaataaccgg caatgccgtt aatgatagcg cggccactta
    18301 tgcccgctac tttaagtagg tttagttttt ggtttatcgg aaaacttaca acaccatagc
    18361 gcacggtacc aattaatgta agcgcggttg cattgccgtc tttaattacg ctaataatac
    18421 tttggcctat ttttaaatgg ttaagctgta cgcttatatt tataatttta gtttcggccg
    18481 gttgcagtgt aatgctttgg gtgttgttta catcggcaat acttttgccg ccataaatta
    18541 cattgccggt aaagtttaca ttgctgtagg ccaccggtgt ggtgttggtg ttggttactt
    18601 taaaggcaaa acttaaatcg gaatttaaaa ttttaatgtt gctcaattta aagtagttgc
    18661 taagctgcca gtttaactgc tttgcactat taataacgtt gcggcccaca gccataaaag
    18721 cccctactaa aagggctgca cctaatacaa tacctgtgcg cttcattatt ttatttttga
    18781 ttttaaaaat ttaacggcct cgtttaatga aataccaata atggcgccta tggtgctgta
    18841 cacaatggtt tgtaatatac ggtcggggta aatgccgtgc cagctaatgg ttaataggct
    18901 gccagcaaat gcagctactt cgttgctatt aaactgctgc ataggcccaa tgtttttttg
    18961 tgcttccatt ttaagtaaag cgcaattata aagcggccgc gcaaggtgtg gcgtggttag
    19021 ttcgttttag ttcatgttag tacactaaaa aaaattatac ggtggtggtg gtgggcttgt
    19081 gtggtttaat ggcttgcaaa aaatcttgct cgtgtatatc ttcatacttg caaaattcgc
    19141 gcaccaatat gcgtgtgccc ggtggtttgt ttagtgccag ctttatatga ttaagttttt
    19201 taatggcttg gcgttggctt acgccatata cttgcatggc atcggttacg tcaacaaaaa
    19261 tacctttcat aaaataattt gtgtgttgtt ttatgggttg caaacttaaa tgatattgaa
    19321 atacgttgta cataaagttt aaattgcttt aaacaaataa ctgtttacat cattatttta
    19381 ttgtaatgga tttgggcggg tgtatttttg atgcaaaaaa taggtttaaa aaggggtatg
    19441 cctgatgctg tgtttttttt tatttcaaaa tggcaccatg cagccactat aaaaagtaga
    19501 aatttatttg tgattttgtg ttttttgtaa tcatgccatt taagtaattg aaaaataaac
    19561 acttacaaaa atatgattta taaatttttg taatacactg gttataatgg cttttttttt
    19621 gtaatcaaat gggcacattt ttgtaatcaa caaaaaaagc ggctgcaaac tgattacaaa
    19681 aaacaatgaa tgattacaaa tttttgtaat caaataaatt aaatttttaa atatttgtaa
    19741 tcaacgcttt gcaatgtttg taggcctttt ggttatgtga ttacaaaatc acaaaaaatt
    19801 ttcactttag ttgctttgta tggcgggggt tgggggggag tgttgccatg tttgccaaaa
    19861 aagcgcggcc gccaaaagtt tgggcgcgta agaaataagg cgtgggcgct aaacaaaaaa
    19921 gcccgcaaag gttgcgggct gtggttgcgt tataattaaa atggtgtggg ctatggtgca
    19981 tttataccaa gcacggccgc tatgcgtagg tagctatcaa tggtatatgc ctttgtgcca
    20041 gcctcgatgc tttgtatttg gtgcagcttt aggcccgctt gtttggcaag ggcatagtag
    20101 gtaagccctg catttagtcg ggcttgttta agcgtggcgc ctatgttttg gcgctgtagg
    20161 tttatgatgg tggtatcaat cattatctat ttgggtatta tctgtttcat caaaacctat
    20221 agcgcggggc ggagcttatc cgcaaaatcc ggcattgccc cggcttaatt aatgcgctta
    20281 tgccttatta tgttgttggc taagcgatac ttatatcgct tatatcgtag ttatgcccca
    20341 tgccaggaga ccgctccgat tgcagagaaa atctcataag cgacttgcgg tacaatggaa
    20401 tttcctaatc ctttaagtct gtccacccga ccgggtagcc tatcaaccac tcgacccact
    20461 ccgggttcag tttcccacca gctactgcat ttaggggcaa agagttccgc tccatctgtg
    20521 attggctgcc gttgttcttc gcatcctgtg tcgttggtgt tggaagaaac atctccacta
    20581 cttgcgttaa acctttttga accagccttc cggttttttt gtcgtaaaaa cgttggttga
    20641 tgttcgttgg atgctccccg ttcttgtcca ctacttctat attcttccat cccggttcct
    20701 gagcttgcgg tgttgggaaa aacagaactt gatttgccag tccagcattg ctgcttcccc
    20761 ctgccttgtt tctcaatcgt atcgttcccg tttccgttac ataaaggctg tccgttttct
    20821 ttttgctcat tttgccgtcc gatgctgttg gcgtaagcca caatccagac cctatctctc
    20881 ctgtgccagg cgtttttacc gcaagctgga ataataaacg tttctgtggt gtagttctgt
    20941 gcttccaagt cagataacac cgtgtcgagt gccaagccga tgatgccagt aacattttcg
    21001 ccaacaacat aggtcggttt aatcgttgca ataactctaa gcatttcttc ccagaggtaa
    21061 cggtcatcgt ctttgccttt tcgctgcccg gcaacgctga acggttggca gggaaatcct
    21121 cctgaaataa cgttaattcg gtttgtgtat tcatatcctg taaagtcttt aatgtctgaa
    21181 aatcgttctg tttttggaaa gtttttagcc aacacctttc tacaccaatc gtctttttct
    21241 acctgaaaaa tattgttcca tcccatccat tgtgctgcaa gatcgaaacc tccaataccg
    21301 ctgaacaaac tcccgtgaga taggcacgag ggcataacat gcgtttggcg caatggcggg
    21361 tgaagtgcat ctatgttact gttgtggttc attgtagttc tgtttttcaa ataagctttg
    21421 gtgctgataa gccgccactg tcgccaagcg caggaccgtt atcggtcatg cctaccatcg
    21481 tgctaaaatg gaaggtcatc acctatttcc tttttggaaa gaactcgttt tattctattt
    21541 ggctgccagt atcttatctg catccataac cagtaacgca tccaatacca aatcttgaca
    21601 aagaataaaa tgattttggt ttgcaatggg aatacatact cgctttcctt attacagaat
    21661 attgaagctg cactatatcc agttagtgtt ttgtattcta ttccaacgtg ccatgagaag
    21721 aaaacaggtt taaccttcac tacctcacca ttccatttgt attttgcttt tagtttcata
    21781 tttaaaattt cgttttaata atccggcacg aaccgcatac gaaatgacat taacaaacac
    21841 gacagaaaag aagggcgaag tgctaacagc ggttttgcgt aatggcgggt gcagtggtaa
    21901 attgaacatt tgtgcttcta tcatcattag tgctaaattg aaagtgagtg ccttgaaacg
    21961 ccaccgagcg taaagcccga aaacgttatg ggcaagtgtt agcaccatac taaaataacg
    22021 acatttgaac attatcatag tttgttttat ggtttgacca aatagcttcc gttgttttat
    22081 acattgaacc tgacattgtt tttataggta aaaatggtag ccaagttgct tttgtgtttt
    22141 cgcaaactat tacttgtccg tttctacttt tacaccattc tgccaagttt tcaaagttaa
    22201 tatttttact actttctttg tagtgttcgc caccaaacat atacggtggg tctataaagt
    22261 aagtagcttc tacattttca atatcttcat acgttcctaa tattatattc cagtgcctaa
    22321 ttttaaataa agattttgca atccgcttta aatctcgttc tacattcaca ccttcaaagc
    22381 tacttacatt cattcgtgga ctttctaatc cgttgcaaac taaaaaacct aaaaagttct
    22441 gctctacttc tgataagtat ttatggtctt taatgctatc accacgtttt aattttggca
    22501 attttaaaat atcattttca cttgcttgtt gtaaatactt ccaagtatca ataagcactt
    22561 tgtatttatc tacaatagtt acttgctttt caaaatactt caatgaatat tttgcacaac
    22621 cagcaaaagg ttcaataacc ttatcaaatt ttggcggtgg ataacaacca accattttac
    22681 ttttacttcc gtagtatgac cacattttta tttcgttttt aattaaccca cacctgcctg
    22741 taacattggt tttgcaatat ggtggcagaa gtgcttcgta tgttgttttt catgctgctt
    22801 ttagttggta gtggctgcgt tgtttgttgc ccgggcgtat acaggattgc aatgggtggt
    22861 gttgtgtggg tgccggttgg catgttgcat ttgttttagt agtgcatggg cttgcattat
    22921 ttttttaaat aggtagctgc ggtgggttac atagtgttac ctgcaagcgg gcgaaccttt
    22981 gctactgtat agcccgcctt cggttagctg caaaacgtta tacgcaagca ctacatttcg
    23041 tttccaaaga gagtttgcgg ttcaaaattt ttattaaaat ctcccacccg ctttaaaata
    23101 atatcgtaat acttttgttc tttttccatt actataaatt gccttttagt attcaaacaa
    23161 gctattgcag tcgttccgct ccctgctgtg ttgtctaata ctacgtctcc ttcatttgtg
    23221 taggttttta ttaagtattc aaataaaggc actggctttt ccgttgggtg cagtttgtct
    23281 tttctatttg cgttgctaac ttcgattatt gaagttgggt agtattcatt attttctact
    23341 acgctattct caaactttcc ataattactt gtgtgcttcc cagtttcggt tgttatccct
    23401 ttctttctta ctttccctct tgtaaccatt tcagggttgt atatcggtag ttttttgtaa
    23461 aagcaaagta tttgttcgtg ccttctcaac ggcatacgtt tagcatttaa aaatccactt
    23521 gaaagttttt tatcccaaat caagtcgtac ttaaataact ttttatttga agttatcagt
    23581 gtgcttgtaa atggttcagc acttgttaaa caaatcattc cattatcttt tacaatcctt
    23641 tcgtactgtt cccaaagttt atcaagtgga ataattacat cccatttatt ttgtgtcgtt
    23701 ccatatggta aatcacaaag tatcatatca atactcccgt ttggtatatc cttcataagt
    23761 tccaaacaat ctcctaataa tatttctttt t
//
LOCUS       DAOMAA010000002         8871 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_4075_length_8871_cov_2.759642, whole genome shotgun sequence.
ACCESSION   DAOMAA010000002 DAOMAA010000000
VERSION     DAOMAA010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8871
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4075_length_8871_cov_2.759642"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="PLO59_00200"
     CDS             <1..302
                     /locus_tag="PLO59_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99538.1"
                     /translation="KIYDHTITGIISGLLAPIIGFYFFKWSFFNYFDNAGFFNHLLGN
                     NLLSPVISISLICNAAAFFFCINRNWLYTGRGIILATFVYGLLIVYLKFFYHTAA"
     gene            318..1424
                     /gene="lpxB"
                     /locus_tag="PLO59_00205"
     CDS             318..1424
                     /gene="lpxB"
                     /locus_tag="PLO59_00205"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HQU99539.1"
                     /translation="MKYYIIAGEASGDLHGSNLIAALKTKDPYFEARGWGGPLMQQQG
                     MSLAKHYKDLAFMGFIEVALNLKTILNNLAFCKKDILQYNPDTLILIDYPGFNLRIAK
                     WAKQHEIKVLYYISPQIWAWKQSRVYTIKKCVDSMYVILPFEKPFYARFNYDVTYVGH
                     PLLDAIEKFNLQEHGKAVKTNKPILALLPGSRQQEIKTMLPIMLQAAKQLPQFHCVIA
                     GAPAITPEFYNQLIKGYEVTVLYNKSYPILKQATAALVTSGTATLETALFHVPQIVCY
                     KGNKLSYYIARQLIKIKYISLVNLIMDKPLVTELIQDNLTANALVQTLNHMLLPDQLK
                     LLMAEYNALTTMLGNSGASQVAANAMYNYLLQSK"
     gene            1428..1943
                     /locus_tag="PLO59_00210"
     CDS             1428..1943
                     /locus_tag="PLO59_00210"
                     /inference="COORDINATES: protein motif:HMM:NF025675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HQU99540.1"
                     /translation="MNQIKVASALTFKPYFRFIFRQNFLKPLPLLIVLFALINLFLLS
                     NYFLKFMYFELKYPWFQMILVLTVFVITPVYFYYINKKHFYKTPDISETKTYDINDDE
                     IKISNVNSNVTIAWNQLYRISESRDFIIIYFDYATAYLINKAHFQSTDDLNTLYAIVK
                     SKPGLLQDMLK"
     gene            1971..4127
                     /locus_tag="PLO59_00215"
     CDS             1971..4127
                     /locus_tag="PLO59_00215"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HQU99541.1"
                     /translation="MKKKTHNHPVKIKPLPFKWNTVPLLRLLIAFVLGIVLQIFMLFD
                     ATTLLTLVSLAVSIFLTIAIYYKYNKNYAKRWLYGFTGSLLFMCLGMSITFVQSHLNY
                     QSHYSNIKSNQYVVKLIEPVVVKPRSVKLIVEVVAAGNPLQRTTGKLLCYLQSSVQAT
                     ALKYGDILVFNGSLQTINAPADPAEFNYKRFLYFHQIFSQVYLKPTQWKLVSSNNGNA
                     LFTYSYELRQKLVNVFKTYLSDTRLIAVASALTVGYDDNIDIELMQAYASAGALHVLS
                     VSGMHVGLVYGVLVFLLAFMDKHKWLKHLKFLLLILFLMFYAILTGMSPSVMRSAAML
                     CFVILGQWIGKQNLVYNTLTISAIVLLCYNPFYITEVGFQLSYAAVFGIVYLHPLINK
                     WYEPTTFIARQIWTITSVSLAAQLLTFPLGLLYFHQFPILFIISNLIVIPCSTVVIFA
                     GIALLLVSFFVGLPVFAWIAVAIAKLLTITISFINFVVLRIDAWPQSIINGISISIAE
                     TWIIYLIIFIGVSFLVQQKAQQLSAILIACIVLACMQLFENFQIYNQKQLVVYSVKKH
                     RAIDFINGKQHYFIADSSLLADKSKLLFHVIHYWWETSVEPKQADISQKHILKDKHYF
                     RNQNIINFNQKKILLIDSNLQQFNTNNKINFDYGIISHCRPMQVIQSTTWANCKQYIA
                     DATLSKKDLNELTAWARKKQIIFYNVQTQGAFMARW"
     gene            4214..6391
                     /locus_tag="PLO59_00220"
     CDS             4214..6391
                     /locus_tag="PLO59_00220"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HQU99542.1"
                     /translation="MKKLFVYLCFVTVTVHAQQNITLEDIFVKGSFRSKSYHIQTLND
                     DKQFVKEEVDSVTKHNNLNCYDILSGAKTKTMFNGSDLKINGKVIDYDNFTLSVDEKK
                     ILFSAETEAIYRHSTQALHYVFDLETKKITDLSTAGKVMLCTLSPDGNKVAYVRDNNL
                     YIRNLTALHEEMITHDGLKNHIINGAPDWVYEEEFSFNKGFEWSPDGKKIAFMRFDES
                     AVKEFTITYYDSLYPRQEKYKYPKAGETNSTVQVIIIDLENGKQTQADLGTEKDQYVP
                     RIGWTANADILYVYKLNRLQNKLDVLTVNNQTGKTQLLFTETDKTYIDIDNSAQIYFT
                     ADGKNFIWSSYESGYNNLWWYDISGKKIKPLTQLKKDVLQFYGYNEKLKTAYYQSYEN
                     TPVDKQIYSVTLNGKTQLLSYNKGSNTAFFSNGFSYFVNAHSDANHAAKYELYKTNTG
                     KVVRLLEDNAALNNKIQSGHLSEKTFFTIPNRNGTALQAWMIKPADFDASKKYPVLMY
                     VYGGPGAPTVNNTAPSGRDFWYYLLAQKGYIIVSVDNRGTTKQGYEFLRSTYMQLGKH
                     ECEDQIDAAKWLGSQAYVDAKRIGIWGWSFGGYLTALCMAKGNDVFKAGMSVAPVTNW
                     RFYDSIYTERYLRTPQQNASGYDDNSPINFAEKIKGRFLLVHGTADDNVHYQNTMMFA
                     NKLIKHNVSFESMNYPNRNHGINGGGATMHIYNLMTNFVLNNL"
     gene            complement(6397..8640)
                     /locus_tag="PLO59_00225"
     CDS             complement(6397..8640)
                     /locus_tag="PLO59_00225"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HQU99543.1"
                     /translation="MSGAFGQSVGLVLSGGGARAYSHIGVLKALEENNIPVHYITGTS
                     MGALVGSMYAIGLSPKQIEDLVTSKEVIDRSNGKKNINQLYFFNDKDPDASWVTIKFS
                     YDSILRMRLPESVIKSSAIDYALMESYAPAVAKAKGNFDSLFVPFRCVASDIKNREPV
                     IFSHGDLARAVRASMAFPFYLSPVAVDGRVMFDGGLYDNFPCDLMLNTYNPDIIIGSS
                     VGNDEAVVVEDNLLSQVRTMIVQRPTNYNVPRPTDILIVPESSVGIFDFEDAKAAIEA
                     GYEATKKQLPLILQSIKTRIYSDTLQLKRAAFFTGQQAIEVGKIEVSGVNKGQQEYIE
                     KTLRQQNKKLISYYLSRNFSRLVANDNIRQIFPILTYNDSTNLYDLRADVKRNKDLQV
                     DFGGNFSSRPINTGFIGVQYQWLNRFSYRAYGNIYFGKLYNSGHASLRIDIPGKHPYF
                     IQPEITFNSFNYYRSSSLLLEDIKPAYLIQDDWKTGIRLGVPSRQIGKIYGDASYFNI
                     RESYYQTRSFSQADTADITKFYGFTVALNYERNTLNKKMYANQGSYISFGLRFVSGDE
                     RTMPGTNSINQDTIRASHQWLQFKFSYENYFAKAGPVRFAFLTELVASTQGFFANYTA
                     SILAAPVFQPIAETKTIFLPQYHSHNYGGLGLKTVYTPFRNFDLRLEGYAFQPYQEIL
                     PGINNDKPNYGDAWAKRYFIGSFDAIYHTPVGPASLGINYYQDFDDPIGIMFHFGYIL
                     FNKRALD"
BASE COUNT         2888 a         1632 c         1590 g         2761 t
ORIGIN      
        1 ataaaatata cgatcatacc attacaggta tcatttcagg attattagct cctatcatag
       61 gcttttactt ttttaaatgg tcgtttttca actactttga taatgccggt ttttttaacc
      121 atttgctggg caataactta ttatcgcctg ttatcagtat cagtttaatt tgcaatgccg
      181 ctgccttttt cttctgcatt aaccgcaact ggctatacac agggcgcggt attattttag
      241 caacattcgt atatggtttg ctcattgtat atttaaagtt tttctaccat acagcggctt
      301 aataaattac tgaatctatg aagtattata ttattgcagg cgaagcatcg ggcgatttac
      361 atggttcgaa cctgatagcg gcattaaaaa caaaagatcc gtattttgaa gcacgcggtt
      421 ggggcgggcc tttgatgcaa cagcaaggta tgagcttggc aaaacattat aaagaccttg
      481 cttttatggg ctttattgaa gttgcattaa atttaaaaac cattctaaac aatcttgcat
      541 tttgtaaaaa agatattctt caatataacc ccgatacttt aattttaatt gattacccgg
      601 gttttaattt acgcattgcc aagtgggcta agcaacatga gattaaagta ttatactata
      661 tatcaccaca aatatgggcc tggaaacaat cgcgtgttta caccataaaa aagtgtgttg
      721 atagcatgta tgtaatactg cctttcgaaa aaccttttta tgcgcgcttt aattatgatg
      781 taacctatgt gggtcacccg cttttagatg ctattgaaaa gtttaattta caggaacatg
      841 gcaaagccgt aaaaaccaac aaaccaattt tagcactatt gcccggcagc cgccaacaag
      901 aaattaaaac tatgttgccc attatgctgc aggcagccaa acaattgcct cagtttcatt
      961 gtgtaattgc cggtgcccca gctatcacac ctgaatttta taatcaatta ataaagggct
     1021 atgaggtaac ggtattgtac aacaaaagct atccgatttt aaaacaagct actgctgcat
     1081 tggttacaag cggtacagca actttagaaa ctgcattgtt tcatgtacca caaattgttt
     1141 gttataaagg caacaaatta tcatactaca tagcgcgcca actgataaaa attaagtata
     1201 taagcttagt taacttaatt atggataagc ctctggttac agagttaatt caggataact
     1261 taactgccaa tgctttagtg caaacattaa atcacatgtt actgcccgat caactgaaac
     1321 tgttaatggc cgaatataat gccttaacaa ccatgctcgg aaatagtggc gcatcgcaag
     1381 ttgcggccaa tgctatgtat aattatttac tgcaatcaaa ataaaatatg aatcaaatta
     1441 aagtagcaag tgcacttaca tttaagccat acttccgatt tattttcaga caaaattttt
     1501 taaaaccatt gcccttatta atagtactgt ttgcattgat aaacctgttt ctgcttagca
     1561 attacttttt aaaatttatg tatttcgaac taaaataccc ttggtttcaa atgatacttg
     1621 tattaacggt ttttgttatt acgcccgttt acttttatta catcaataaa aaacattttt
     1681 ataaaacgcc tgatatttcc gaaaccaaaa cttacgatat aaatgacgat gaaataaaaa
     1741 ttagtaatgt aaacagtaat gtaactatag cctggaatca gttatacaga atatccgaat
     1801 cgcgcgattt tattattatt tattttgatt atgcaactgc gtacctgatt aacaaagcac
     1861 actttcaatc aacggacgat ttaaatacgc tttatgctat tgttaaatcg aaacccggct
     1921 tgttgcagga tatgttgaaa taatcacttt tataatttat tttagatggg ttgaaaaaga
     1981 aaacccataa ccatccggta aaaataaaac cgttaccatt taaatggaat acagttccat
     2041 tgctcagact gctgatagca tttgtattgg gcattgtgtt gcaaattttt atgctgtttg
     2101 atgccactac cctgcttaca ctagtaagct tggccgtttc aatttttcta actatagcaa
     2161 tatactacaa atataataag aactacgcaa agcgttggct atatggtttt acgggctcat
     2221 tgctgtttat gtgtttgggt atgagcatca cttttgtgca aagccattta aattatcaaa
     2281 gtcactattc aaatattaaa tcaaatcaat acgttgtaaa attaatcgaa cccgttgtag
     2341 ttaagcctcg ttcagttaaa ctaattgttg aagttgtagc tgcaggcaat ccattacaac
     2401 gtactaccgg caaattgctt tgctatctgc aatcatctgt acaagcaact gcgctaaaat
     2461 atggtgacat tttagttttt aacggctcgc ttcaaaccat taatgccccg gctgaccctg
     2521 cagaatttaa ctacaaacga tttttatact tccatcagat atttagccag gtgtatttaa
     2581 agcctacgca atggaagtta gttagcagca acaacggcaa tgctttgttt acatactctt
     2641 acgaactacg ccaaaagctt gttaacgttt ttaaaacgta tttgagcgac acacgtttaa
     2701 ttgcagttgc atctgcctta accgtaggtt atgacgataa tattgacata gaattaatgc
     2761 aagcctatgc aagtgctggt gcattacatg tgctaagtgt ttcgggtatg catgtgggcc
     2821 ttgtgtatgg tgtattggtt tttttgttgg catttatgga caaacacaaa tggctaaaac
     2881 atctgaaatt tctgttgctc attttatttt taatgttcta tgccatatta accggcatgt
     2941 cgccaagcgt tatgcgctcg gcagctatgc tttgctttgt gatattgggg caatggattg
     3001 gcaaacaaaa tttagtgtac aatacactta ccatatcggc cattgtactg ttatgctata
     3061 acccttttta cattaccgaa gttggttttc agctttctta tgctgccgtt tttggtattg
     3121 tttatctgca tccgctcatt aataaatggt atgaacccac aacatttata gcccgccaaa
     3181 tctggacaat tacaagtgta tcgttagcag cacaattatt aacttttcca ttagggttgt
     3241 tatattttca tcagtttccc atactgttta tcatatctaa tttaattgtg ataccctgca
     3301 gtacagttgt aatttttgca ggcattgcat tgctacttgt ttcctttttt gttggcttgc
     3361 cggtatttgc ctggatagcc gttgcaatag ccaagttact aacaattact atctcattca
     3421 ttaattttgt ggttttaaga attgatgcat ggccacaaag tattattaac ggcatttcga
     3481 tttccattgc cgaaacatgg ataatttacc tgataatttt tattggtgtt tcgtttttgg
     3541 tgcaacaaaa agcccaacag ttaagtgcta ttttaattgc ctgtattgta ttagcatgta
     3601 tgcaactatt tgaaaacttt caaatttaca atcaaaaaca actggtagtt tacagtgtaa
     3661 aaaagcacag agccattgat tttattaatg gcaaacagca ttactttatt gcggatagca
     3721 gtttgttggc cgataaaagt aaattactgt ttcatgtgat acattactgg tgggaaacat
     3781 cagtagagcc caagcaagct gatatatcac aaaagcatat tttgaaggat aaacattatt
     3841 tcagaaacca aaatattata aatttcaacc aaaaaaagat attgctaatt gatagcaacc
     3901 tgcaacaatt taatacaaac aataaaatta actttgacta tggtatcatt agtcattgca
     3961 ggccaatgca agtaatacaa agcacaacct gggccaattg caagcaatac atagccgatg
     4021 caactttatc gaagaaagat ctgaatgaac taactgcttg ggcaagaaaa aagcaaataa
     4081 tcttttataa tgtgcaaaca caaggcgcat ttatggcgcg ctggtaactt tactttaacc
     4141 aattagcatg ctattaagta ttggcctaaa aaccaaataa tgtaggtttg acacaactta
     4201 aacacctatc gttatgaaaa aattgtttgt ctatttatgt tttgtaacag ttactgtaca
     4261 tgcacagcaa aatattaccc ttgaagacat ttttgttaaa ggcagtttca gatcaaaatc
     4321 gtaccacatt caaactctga acgatgataa acagtttgtt aaagaagaag ttgatagcgt
     4381 aaccaagcat aataatttaa attgttatga catcctatcg ggagctaaaa caaaaacaat
     4441 gtttaacgga agtgatttaa aaatcaacgg taaagttatt gactacgata attttacgct
     4501 aagcgttgac gaaaagaaaa tattattcag tgccgaaacc gaagccatat accgtcatag
     4561 tacacaagcg ctgcattatg tgttcgattt ggaaaccaaa aaaattaccg acctgtcaac
     4621 tgcaggaaaa gttatgcttt gcacactatc acccgatggc aataaagttg cgtatgtacg
     4681 cgataataat ttatatatca gaaatctgac cgcattgcat gaagaaatga taacacatga
     4741 tggtctaaaa aatcacataa ttaatggggc gcctgactgg gtttatgaag aagagttttc
     4801 gtttaacaaa ggttttgagt ggagccctga tggtaaaaaa attgcattca tgcgctttga
     4861 tgaatcggca gtgaaagaat ttacaattac ctattatgac tcattgtatc cgcgtcagga
     4921 aaaatacaaa taccccaagg ccggtgaaac aaacagtact gtacaagtga taattattga
     4981 tttagaaaat ggcaaacaaa cccaggccga tttaggtact gaaaaagacc agtatgtacc
     5041 acgtattggc tggactgcca atgccgacat attatatgtt tacaaactta accgcctgca
     5101 aaacaaatta gatgttttaa ccgtaaacaa ccaaacaggt aaaacccaat tactatttac
     5161 cgaaacagac aaaacctaca ttgacattga caacagtgca caaatatatt ttacagcaga
     5221 tggtaaaaat tttatttggt cgagttatga aagtggatac aataatttat ggtggtatga
     5281 tataagcggt aaaaaaatta aacctttaac acaactaaaa aaggatgtac tacagtttta
     5341 tggttataac gaaaaactaa aaacagccta ttaccaaagc tatgaaaaca ctccggttga
     5401 taaacaaatt tatagtgtaa cgcttaatgg caaaacccaa ttgcttagtt acaataaagg
     5461 aagcaatacc gcctttttta gcaatggatt ttcatacttc gttaatgccc acagcgatgc
     5521 caaccatgct gccaaatatg agttgtataa aacaaataca ggcaaagttg ttaggttgtt
     5581 agaagacaat gctgcactaa acaataaaat acagagtgga catttaagcg aaaaaacctt
     5641 ttttactata cccaaccgaa atggcacagc cttgcaagca tggatgatta aacctgctga
     5701 ttttgatgca tcaaaaaagt atccggtact aatgtatgtg tatggtgggc cgggtgcgcc
     5761 tacggttaat aatacggcac catcgggacg agatttttgg tattacctgt tggcacaaaa
     5821 aggttacatt attgtttcgg ttgataacag aggaactacc aaacaaggct atgagttttt
     5881 acgaagcact tacatgcaat taggaaaaca tgaatgcgaa gatcaaattg atgctgctaa
     5941 gtggttgggt agccaggctt atgtggatgc aaaacgtatt ggtatttggg gctggagttt
     6001 tggcggatat ctgacagcgc tttgcatggc aaaaggcaat gatgttttta aagcaggcat
     6061 gtcggtagca ccggttacga attggcgctt ttatgatagt atttatacag agcgttattt
     6121 acgtacacca cagcaaaacg cttcgggtta tgatgataat tcgcccataa attttgccga
     6181 aaagattaaa ggccgttttt tattagtgca tggcacagcc gatgataatg tacattatca
     6241 aaacaccatg atgtttgcaa acaaacttat taaacacaat gttagtttcg aaagcatgaa
     6301 ttatccaaac cggaaccacg gcattaacgg gggtggtgct accatgcata tatataattt
     6361 aatgacaaac tttgttttaa ataacttata gcatttttaa tccaaagctc ttttattaaa
     6421 tagtatgtat ccgaagtgga acataatgcc tattgggtcg tcaaaatcct gataatagtt
     6481 aatacctaaa ctggccggac caaccggtgt gtggtaaatg gcatcgaatg acccaataaa
     6541 ataacgctta gcccaggcat cgccatagtt gggtttatca ttattaatac ccggcaatat
     6601 ttcctgataa ggctgaaacg cgtaaccctc taatcgcaaa tcaaaattac ggaaaggagt
     6661 ataaacagtt tttaaaccta atccaccata attatgtgaa tgatattgtg gcaaaaaaat
     6721 agttttggtt tcggctatcg gttgaaaaac cggtgcagcc aaaatacttg ctgtgtaatt
     6781 ggcaaaaaaa ccttgcgtac ttgcaaccag ctcggttaaa aatgcaaatc gaacggggcc
     6841 tgcttttgca aagtagtttt cgtacgaaaa cttaaattgt aaccattggt gtgatgccct
     6901 tatcgtatct tggtttatac tgtttgtccc gggcatggtg cgctcatcgc ccgaaacaaa
     6961 acgcaagccg aatgaaatgt atgagccttg atttgcatac atttttttat taagcgtatt
     7021 gcgctcataa tttaacgcca cagtaaatcc ataaaacttg gttatgtcgg ctgtatcggc
     7081 ttgcgaaaaa cttcgtgttt gatagtagct ttcacggata ttaaagtaag atgcatcgcc
     7141 ataaatttta ccaatttgtc gtgatggaac acccaaccga atgcctgttt tccaatcgtc
     7201 ttgaattaag tatgcaggct taatatcttc taataacaaa ctgctgctgc ggtagtagtt
     7261 aaagctgtta aaagtaattt caggttgtat aaaatacgga tgcttgcccg gtatatcaat
     7321 acgcaaactt gcatgacctg aattataaag tttgccaaag tatatattac cgtatgcacg
     7381 ataggaaaac cggtttagcc attgatattg cacaccaata aaacctgtat taataggtct
     7441 tgatgaaaaa ttgccgccaa aatcaacctg caaatcttta ttgcgtttta catctgcacg
     7501 taaatcatac aaattagttg agtcattata tgtaaggatg ggaaaaatct gacgtatgtt
     7561 atcattggct actaagcgcg aaaagttgcg cgataaataa taggatatca gctttttgtt
     7621 ttgctgacgc agtgtttttt caatatactc ttgctggcct ttattaacgc cactcacttc
     7681 aattttacca acttctatgg cttgctggcc ggtaaaaaag gcagctcgtt ttaattgcaa
     7741 cgtgtcggaa taaatacgtg tttttatact ttgcaaaatt aatggcaatt gtttttttgt
     7801 tgcttcataa cctgcttcta tagcggcttt tgcatcttca aaatcaaaaa ttccgaccga
     7861 gctttcaggc acaataagaa tatctgtggg cctcggtacg ttatagttag ttggtcgttg
     7921 tacaatcatg gtgcgcactt gcgatagtaa attatcttca actaccactg cttcatcatt
     7981 gccaaccgaa cttccgatta ttatatcggg gttataggta tttaacatca aatcgcatgg
     8041 aaagttatcg tataaaccgc catcaaacat aacacgacca tcaaccgcta caggcgataa
     8101 ataaaatggg aatgccatag aagcacgcac agcacgtgcc aaatcgccat gcgaaaaaat
     8161 aaccggctct cggtttttta tgtcagatgc cacacacctg aagggaacaa acagcgaatc
     8221 aaaattacct tttgcttttg caactgcagg tgcatagctc tccataaggg cataatcaat
     8281 tgcacttgac ttaataacgc tttcgggcaa gcgcatgcgc aaaatactat cgtacgaaaa
     8341 tttaattgtt acccatgatg catcgggatc tttatcatta aaaaaataca actgattgat
     8401 attttttttc ccgttcgaac ggtcgataac ttctttgctg gtaactaaat cttctatttg
     8461 tttaggcgat aagccaatgg catacatact gcccaccaat gcacccatac tggtgccggt
     8521 tatataatgc acaggtatat tattttcttc gagggctttt agtacaccta tgtgtgagta
     8581 agcgcgtgct ccgccaccac ttaaaaccaa acctacactt tggccaaatg caccactcaa
     8641 attggtaatt ataaacagga tgataagtag tcgtaccagc atacgcatca ataacgatta
     8701 cgaaattaga aaattgaaag gcttgtaatt ttttttactt aaatgttatc agcaagtgtt
     8761 atttgataac tccaaacccg gcaaaacgat ttttccaata gggttcattt acatcgctta
     8821 ttataatgcc ttttgatgag cttgcatgtg caaatttgtt atcgcccaga t
//
LOCUS       DAOMAA010000003         7414 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_5371_length_7414_cov_3.077864, whole genome shotgun sequence.
ACCESSION   DAOMAA010000003 DAOMAA010000000
VERSION     DAOMAA010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7414
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5371_length_7414_cov_3.077864"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..1277
                     /gene="atpB"
                     /locus_tag="PLO59_00230"
     CDS             87..1277
                     /gene="atpB"
                     /locus_tag="PLO59_00230"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018343160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HQU99544.1"
                     /translation="MKSLVALSIEKVLSLKTLVLIVFLACANAVCLASSESTEHTDAN
                     NHATETSHDAAGHATEKFNPGKTIIDHVIDAHDWHIAGEGANSITIPLPIIIYSSQRG
                     LDFFMSSHFHHGHQTYNGYMLQGHKVVAVNETESIDAHHATIDQVTTDALLDFSLTKN
                     AFSIMLSCLIMFFLFLSVAKAYGKRTGQAPKGLQSLMEPLILFVRDDIAKTNIGEKHY
                     KRFLPFLLSVFFFIWLNNLLGLVPVFPGGTNITGNISVTLTLAVLVFIITTLNGTKDY
                     WMHVFAMPGVPKGVLLILTPIEILSIFLKPFVLMVRLFANITAGHIIALSFFSLIFIF
                     GEMSAAGGYGVSVFTIAFTIFMGALELLVAFLQAYVFTLLTSLYFGAALESHDDHAHA
                     HDAH"
     gene            1324..1590
                     /gene="atpE"
                     /locus_tag="PLO59_00235"
     CDS             1324..1590
                     /gene="atpE"
                     /locus_tag="PLO59_00235"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020528973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HQU99545.1"
                     /translation="MLSSILLQAAETASNIGIGYGLAGLGAGLAAVGAGIGIGRIGGS
                     AMEGIARQPEAVGDIRSNMIVAAALVEGAAFFAMVIGLLVILTK"
     gene            1709..2203
                     /gene="atpF"
                     /locus_tag="PLO59_00240"
     CDS             1709..2203
                     /gene="atpF"
                     /locus_tag="PLO59_00240"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HQU99546.1"
                     /translation="MELLTPSYGLIFWMLVWFGIMFFLLKKFAWPVILNGLQERERSI
                     SDALDAAKNAKEEMVRLKSDNEKILAEARSQRDTLLKEARDTKEAIINEAKSKAQAET
                     DRLLTQAREAIISEKNAAVTELKNQVSSLAIEIAEKVIQRELNSPEQHKALVNDMIKD
                     LKFN"
     gene            2231..2785
                     /gene="atpH"
                     /locus_tag="PLO59_00245"
     CDS             2231..2785
                     /gene="atpH"
                     /locus_tag="PLO59_00245"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HQU99547.1"
                     /translation="MSATQIPYRYAKALFDYAKTNQQLDRVFADMQLINKLVDENREL
                     DRVFHSPILKPTKKAEVFNAVFKNLLSKQTLDFVDMLIDKGRENYMEAIAESFMQMHN
                     EANNVIEAQITTAVAIDEKLRSEIKTKLKAQLNRDIVLIEKVNPEILGGYILRVNDKQ
                     EDKSLTSKLNNLKRGFEANRMNAR"
     gene            2829..4409
                     /gene="atpA"
                     /locus_tag="PLO59_00250"
     CDS             2829..4409
                     /gene="atpA"
                     /locus_tag="PLO59_00250"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HQU99548.1"
                     /translation="MAEIKPAEITAILREQLSNFKTAAELEEVGTVLQVGDGIARIYG
                     LDKVQSGELIEFQNGVKGIVLNLEEDNVGAVLLGPQGNLKEGDSAKRTGLIASIKVGE
                     GMLGRVVDALGNPIDGKGPITGEVVEMPIERKAPSVIFRQPVTEPLQTGIKAIDAMIP
                     IGRGQRELVIGDRQTGKTAVCIDTIINQREFYDKGQPVFCIYVAVGQKGSTVAQIVKT
                     LEERGAMPYTVVVAATAADPAPMQFYAPMAGAAIGEYFRDTGRPALIVYDDLSKQAVA
                     YREVSLLLRRPPGREAYPGDVFYLHSRLLERAAKIINNDEIAANMNDLPAPLKGKVKG
                     GGSLTALPIIETQAGDVSAYIPTNVISITDGQIFLEANLFNSGVRPAINVGISVSRVG
                     GNAQIKSMKKVAGTLKLDQAQYRELEAFSKFGSDLDATTKAVLDKGARNVEILKQGQY
                     SPLRVEQQVAIIYLGTKGLLRNVPVNKVREFEATFLGVMESKHKDVLELLRQGQLTDA
                     VTSTIEAVGKELATSYAN"
     gene            4479..5363
                     /gene="atpG"
                     /locus_tag="PLO59_00255"
     CDS             4479..5363
                     /gene="atpG"
                     /locus_tag="PLO59_00255"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HQU99549.1"
                     /translation="MANLKEIRIRIASVQSTQQITKAMNMVSAAKLRKATDAIMQMRP
                     YANKLQEVLQNVSGTLDASTGGAFAAVRPVKKVLIVAVTSNRGLCGGFNANIIRSVNN
                     IIAENYAPQAAAGNVKVMCIGKKAADFFSKNKNLFLSSHSEVFANLSFDSVAPIADHI
                     MDEFATGNYDKVEVVYNRFKNAVVQVVTREQVLPIVQAETTDAKKGMINDYIFEPDKE
                     SIVLDLIPKSIKIQLYKATLDSHASEHGARMTAMSKATDNAGELLRSLRIFYNKARQA
                     AITNEILEIVGGANALKG"
     gene            complement(5447..5794)
                     /gene="ssb"
                     /locus_tag="PLO59_00260"
     CDS             complement(5447..5794)
                     /gene="ssb"
                     /locus_tag="PLO59_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015481579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HQU99550.1"
                     /translation="MNNLRNSVRLIGYLGSNPELKEFEKGGKVARINIATNESYTNQK
                     GEKVVDTTWHTCVAWGKSADYAAKYFTKGSEVAIEGRLTQRQYTDKEGAKKYITEIVV
                     NEMMVCGKKAPTE"
     gene            complement(6231..6407)
                     /locus_tag="PLO59_00265"
                     /pseudo
     CDS             complement(6231..6407)
                     /locus_tag="PLO59_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018967179.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
     gene            complement(6542..6841)
                     /locus_tag="PLO59_00270"
     CDS             complement(6542..6841)
                     /locus_tag="PLO59_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylpyrophosphate synthetase"
                     /protein_id="HQU99551.1"
                     /translation="MKLYTTLSEAINDLTKKGYITNFNINQSCIECGESKVQLQPSEF
                     EIERVYRFQDMSDVDNEDILYVISSKKNNMKGLLVNAYGIYSDSTATSLIDKLKI"
     gene            complement(7030..7251)
                     /locus_tag="PLO59_00275"
     CDS             complement(7030..7251)
                     /locus_tag="PLO59_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002663009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99552.1"
                     /translation="MENKEIKFKTNLNCGGCVLKVQADLNNHVGVSKWNVDISNPDKI
                     LTVQMEGITEYEVIGIIKSKGFKAEALND"
BASE COUNT         2296 a         1461 c         1424 g         2233 t
ORIGIN      
        1 gacctttgtt tagtcaaaaa acttttttca gaaagtttaa tttatacttt tgcgcccgag
       61 ttttaaaaac cctatcagcc ataaacatga aaagccttgt agcactaagc attgagaagg
      121 tattaagcct taaaaccctt gttttaattg tgtttttagc ctgtgctaac gctgtatgcc
      181 ttgccagttc tgaatcaaca gaacatactg acgcaaacaa ccatgcaact gagacaagcc
      241 atgatgcagc cggccatgca actgaaaagt ttaatccggg caaaacaatc atcgatcacg
      301 taattgatgc acacgattgg cacattgccg gtgaaggtgc aaacagcatt accattccgc
      361 taccaataat tatttatagc agtcagcgcg gtttggattt ttttatgagc agccactttc
      421 atcatggcca tcaaacttat aatggctaca tgttacaagg tcataaagtg gtagctgtaa
      481 atgaaaccga gtcaattgac gctcaccacg caaccattga ccaggtaacc accgatgccc
      541 tgttagattt ctcgcttacc aaaaatgcat tcagcattat gctaagttgc ttaataatgt
      601 tctttttgtt tttgtcggta gcaaaagctt acggcaaacg taccggacaa gcaccaaaag
      661 gactacaatc gcttatggag ccgctaatat tatttgtgcg cgatgatata gctaaaacca
      721 acattggcga aaaacattac aaacgttttc tgccattttt attatcggta ttcttcttca
      781 tttggcttaa caacctgtta ggtttggttc ctgtgtttcc gggaggtacc aatataaccg
      841 gcaacatttc tgttaccctt actttggctg ttttggtatt tataataacc accttaaatg
      901 gaaccaaaga ttactggatg cacgtatttg caatgcccgg tgtgccaaaa ggggtgctac
      961 tcattttaac accaatagaa attctaagta tattccttaa accatttgta ttgatggtcc
     1021 gtttgtttgc taacataaca gcaggccaca ttattgcctt atcattcttc tcattgattt
     1081 ttatatttgg tgagatgagt gcagcgggtg gctatggcgt atcggttttc acaatagcct
     1141 ttaccatttt tatgggtgca ttggagttat tggttgcatt tcttcaggcc tatgtattta
     1201 ccttattaac ctcattatat tttggtgctg cactcgagtc gcacgatgat catgcacatg
     1261 cacacgatgc acattaattc aattttaaat aaacgatttt aaacacttta atacttacac
     1321 attatgttat catcaatttt attacaagct gccgaaactg catcaaacat tggtatcgga
     1381 tatggtttag ccggtttagg tgctggttta gctgcagtag gtgccggtat cggtattggc
     1441 cgtattggtg gcagtgctat ggaaggtatt gcacgtcagc ccgaggctgt aggcgacatt
     1501 cgttcgaaca tgattgttgc tgccgctctt gttgaaggtg ctgcattctt tgcaatggta
     1561 attggcttat tggttatttt aaccaagtaa tacaagctta ttacggtaaa acacaaacct
     1621 catttgtaac gcttggttac aaatgaggtt gttttaagta atcacacaag tgtttcaaac
     1681 aataaaacaa ctgtcaaaaa aataaaacat ggaattactc acgccctcat acggactcat
     1741 attttggatg ctagtttggt ttggcatcat gttttttctg ttaaagaaat ttgcctggcc
     1801 tgtaatttta aacggattgc aggagcgcga gcgctcaatt tctgacgcat tagatgcagc
     1861 taaaaatgcc aaagaagaaa tggttcgact aaagtcggat aatgaaaaaa tattggccga
     1921 agcacgcagc caacgcgata ctttattaaa ggaagcccgc gataccaaag aggctatcat
     1981 aaacgaagcc aaatcaaaag cccaagccga aaccgaccgc ctgcttaccc aagcgcgcga
     2041 agccataatc agcgaaaaga atgctgctgt aaccgagttg aaaaatcaag tatcgtcact
     2101 ggcaattgaa attgctgaaa aggtgattca acgtgaatta aacagccccg agcaacacaa
     2161 agccttggtt aatgatatga ttaaggacct aaagtttaat taataaacac actgcttaaa
     2221 gttaaacagc atgagtgcca cacaaatacc ctaccgctac gccaaagctt tatttgatta
     2281 cgcaaaaacc aatcaacagc tcgaccgtgt gtttgccgat atgcaactga taaataaatt
     2341 agttgacgaa aaccgcgaac tcgatcgcgt gtttcatagc cctattttaa aacctaccaa
     2401 aaaagcagaa gtatttaatg ccgtttttaa aaacttgcta tctaaacaaa cattagattt
     2461 tgtggatatg cttattgata aaggccgcga aaactacatg gaagcaattg ccgaatcgtt
     2521 tatgcaaatg cacaatgagg caaacaatgt aattgaagca caaattacta ctgcagttgc
     2581 aattgacgaa aaattgcgta gtgagattaa aacaaaatta aaagcgcaat taaaccgcga
     2641 tatcgttttg atagaaaaag taaatcctga aatattggga ggttatatct tacgtgttaa
     2701 tgataaacag gaagataaaa gccttacctc taaactaaat aatttaaaac gcggcttcga
     2761 agccaacaga atgaatgccc gttaaaggca ataattaata aaaacgctac cagtaaacac
     2821 caaacactat ggcagaaata aaaccggctg aaattacagc catcttgcgc gagcaactat
     2881 caaactttaa aaccgcagcc gaacttgaag aagtaggtac tgtactacaa gtaggtgatg
     2941 gtattgcccg tatttacgga ttggataaag tacaatcagg cgaacttatt gaatttcaga
     3001 acggtgttaa aggcattgta ttaaacctgg aagaagataa cgtaggtgct gtattacttg
     3061 gccctcaagg taatttgaaa gaaggcgact cagccaaacg taccggctta atagcctcta
     3121 ttaaagtagg cgaaggtatg ttaggccgcg tagttgatgc tttaggaaac cctattgacg
     3181 gcaaaggacc cataacaggt gaagttgtag agatgcctat cgaacgcaaa gcgcccagtg
     3241 taattttccg ccagcctgtt accgaaccac tacaaacggg tattaaggct attgatgcca
     3301 tgattcctat tggccgtggc caacgcgaat tggttattgg cgaccgtcaa accggtaaaa
     3361 cagcagtttg tattgatacc atcataaacc aacgcgagtt ttatgacaaa ggtcaaccgg
     3421 tgttttgtat ttatgttgcc gtaggacaaa aaggttctac cgttgcacaa atagtaaaaa
     3481 ctttggaaga acgaggtgcc atgccctata cagtagttgt tgcagcaact gcagccgacc
     3541 ctgctcccat gcagttttat gcaccaatgg caggtgctgc aattggcgaa tattttagag
     3601 ataccggccg cccggcttta attgtttatg atgatttatc aaaacaagct gtagcttatc
     3661 gcgaagtatc acttttatta cgcagaccac ccggacgcga agcatatccc ggtgacgtat
     3721 tttatttaca tagccgttta ttagagcgtg ctgcaaaaat tatcaataac gatgaaattg
     3781 cagccaacat gaacgactta cctgcaccac ttaaaggcaa agttaaagga ggcggttcat
     3841 taacagcttt acctattatc gaaacacaag ccggtgacgt ttcggcctat atccccacta
     3901 acgtaatttc tattaccgat ggtcagatat ttttagaggc aaacttattt aactcaggtg
     3961 ttcgtcctgc tattaacgta ggtatcagcg tatcgcgtgt tggaggtaat gcccaaatta
     4021 aatccatgaa aaaagtagcc ggtactttaa agctcgatca ggcacagtac cgcgaactag
     4081 aagcatttag taaattcggt agcgatttag atgccactac taaagcagtt ttggataaag
     4141 gtgcgcgtaa cgtggagata ttaaaacaag gccagtattc gcctttgcgt gttgaacaac
     4201 aagtagcaat tatttactta ggtactaaag gattactacg taatgtacct gtaaataaag
     4261 tgcgcgagtt tgaagctacc ttcttaggcg ttatggagtc gaaacataaa gatgtattgg
     4321 aattattgcg ccaaggccag ttaaccgatg ccgtaacatc aaccattgaa gcggttggga
     4381 aagagttggc aacatcgtac gcaaactaaa tttaaaatta aaccgtaagg caaaatgcag
     4441 taagcaaagt agccagcaaa ccagcaaata ataacagcgt ggctaactta aaggaaatac
     4501 gaatacgcat tgcatcggtg caatcaacac agcaaattac caaggctatg aacatggtaa
     4561 gtgctgccaa attgcgtaaa gctaccgatg cgattatgca aatgcgtccc tatgctaata
     4621 aactgcaaga ggttttgcaa aatgtttcgg gtacattgga tgcaagtacc ggtggtgcat
     4681 ttgcagccgt gcgaccggta aaaaaagtgc ttattgttgc agttaccagt aatcgtggat
     4741 tatgtggtgg ttttaatgcc aacattatca ggtcggtaaa caatatcata gccgaaaact
     4801 atgcaccaca agccgctgca ggcaatgtaa aagtgatgtg cattggaaaa aaagcagccg
     4861 actttttcag caaaaacaaa aacctgtttt tatcaagcca tagcgaagta tttgccaatc
     4921 tttcatttga ctcagttgca cccattgccg accatattat ggacgaattt gcaaccggta
     4981 attatgataa ggttgaagtg gtttataatc gttttaaaaa tgcagttgtg caggttgtta
     5041 ctcgcgaaca agtattaccc atagtacaag ccgaaacaac agatgcaaaa aaaggcatga
     5101 taaacgatta tatttttgaa ccggataaag agtcaatcgt attagactta atccctaaat
     5161 caataaaaat acaattgtat aaagccacac ttgattcgca tgcaagtgag cacggtgccc
     5221 gtatgacagc catgagtaaa gcaaccgata atgcaggtga gttgctacga agcttacgca
     5281 tattttataa taaagcacgt caggcagcta ttacaaacga aattcttgaa attgttggtg
     5341 gcgcaaatgc tttaaaaggt taaacgttat cataaataaa aaaagggctg tgattacaat
     5401 cacggccctt ttttttgctg catactttta gttttaatgc attaaattat tcagttgggg
     5461 cttttttacc gcaaaccatc atttcgttta caacaatttc agttatatac tttttagcgc
     5521 cttctttatc ggtgtactga cgttgggtaa ggcggccttc aatggctact tcactgcctt
     5581 tggtaaaata cttggctgca taatcggcac tttttcccca tgccacacag gtatgccagg
     5641 ttgtgtcaac aaccttttcg cctttctgat tggtgtatga ttcgttagtg gcaatattga
     5701 tgcgggcaac cttaccacct ttttcaaatt cttttaactc gggattgcta cctaaataac
     5761 cgattaagcg tacactgttt cttaaattgt tcatgacttt actgttttaa atgatttata
     5821 atttttattg tttgatgccg acaaatgaag caccaaccat aataccaagt cggacgattt
     5881 ccgtttactt ctgataaaaa acggatttaa ccgtttgtaa tcggtctgtt gcctgcaacc
     5941 atttggtcgc ctattaacat tgaactgacg gactttaacc gaacactttt ttgaaaactg
     6001 acttgacgac cagaccgctt ctaacaaaca gcacaacgtt tcgtgttttc ttcttcttca
     6061 tacaattgtt ctttgagcgt ggtaacaatg ccatgaaatc gtttctcttt ttggtcgttc
     6121 ttagagaatg tttcatgaag ataatccaat gaaactttaa atagttcttt atccaaagcc
     6181 aaagccactt tatgttcatg ctctggataa tctatttctt ttaatgctgc ttttctgata
     6241 aaaaacggca gcgttaatcc ctgaatggtt agggttaaaa ggattacaat aaaggtgata
     6301 aacaagatta aatttctttg cggaaaatcg ctgccatctt ataagtatag tggaatagaa
     6361 agtgcagcag ctaatgatac tacacctcgc ataccagccc atgtgatcta tgtaaataaa
     6421 tggccctacc atgccgttct gccgtatagg aagttgtcag cctacaagaa atttactgat
     6481 gtgggttata ctttattcta ttttaaattt gtgttttaca tattgcactt gcgattttta
     6541 tttagatttt caatttatca atcaaacttg ttgctgtgct atctgagtat attccatagg
     6601 catttaccaa aagacctttc atgttatttt tcttggaaga gataacgtat aagatgtctt
     6661 cattatcaac atcggacatg tcctgaaacc gatacaccct ttcaatttca aactcgcttg
     6721 gttgaagttg aactttactt tcaccacatt caatacagct ttgatttatg ttgaagttag
     6781 tgatataacc tttttttgtt aggtcgttga tcgcttctga tagtgttgtg tataatttca
     6841 ttattcagaa attttaaggt tcgtttggta aaagtgcaac aatttattta ctatttctga
     6901 aacatattca ggcttttaac aggttcgaat attggtttcg ccattctcca caaacaagtc
     6961 tttatttttg ttcccgaagc tttcgcaaat gctttatccg ttatataaac caccatgcta
     7021 aagagaaaat taatcgttca acgcctccgc cttaaacccc ttgcttttaa taatgccaat
     7081 tacttcatat tcggtaatgc cctccatttg caccgttaat attttatctg gattgctgat
     7141 atctacattc catttagaaa caccaacatg gttatttaaa tcggcttgaa cttttaatac
     7201 acatccacca caattaagat tggttttgaa ttttatttct ttgttttcca ttgttctgat
     7261 ttttatatgt tgtttgatac tgcaaatttc gaaagagtac agcgatggac tgttgcgtta
     7321 tttcttattt gatttgtaag ttttactttt tccatttcaa ttgcaaactg ttactcacaa
     7381 cactcacact gcccaaagcc attgctgcgc ctgc
//
LOCUS       DAOMAA010000004         7082 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_5758_length_7082_cov_3.344101, whole genome shotgun sequence.
ACCESSION   DAOMAA010000004 DAOMAA010000000
VERSION     DAOMAA010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7082
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5758_length_7082_cov_3.344101"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..1406
                     /locus_tag="PLO59_00280"
     CDS             9..1406
                     /locus_tag="PLO59_00280"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HQU99553.1"
                     /translation="MNCPAVVYFTTDKKTLPTVLSAAEAGFVKAELAAEKKLIVVNQY
                     NKHVFIQLIEKKDKNVLMESLRKAGSQMADMINKLKVTEVLLTDAKLDDELLAVAEGM
                     ALANYQFLKYKTQAEKEKNKLQTIFLHADALKQKPKKVDQLQILVDSVCRARDLVNEP
                     VIYLTATRLAEAFKKMGQEAGFKVEIFNKTKIESLKMGGLLAVNYGSVEPPTFTVMEY
                     KPRNANNKRPYVLVGKGVVYDTGGLSLKPTPNSMDYMKCDMGGSAVVGATLYAIAKAK
                     LPIHVIGLVPATDNRPGGNAYTPGDVITMMSGLTVEVLNTDAEGRMILADALHFAKQY
                     DPKLVCEFSTLTGAAAAAIGQYGIVCMGTADEKVKNKLKESGDAVHERLAEFPFWDDY
                     DELIKSDIAEIKNIGGPYGGAITAGKFLSRFADYPFMHFDIAGPAFIKGNDSYRGKNG
                     TGVGVRLMFDFLKKL"
     gene            complement(1799..2572)
                     /locus_tag="PLO59_00285"
     CDS             complement(1799..2572)
                     /locus_tag="PLO59_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009279736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HQU99554.1"
                     /translation="MKAIIIEDEKLIARELQFKINAVVPDVEIIEILPSLKTSKRWLM
                     NNAEPDLIFMDIQLSDGISFEIFETFKLSCPVVFTTAYDEYAMRAFKVNGVDYLLKPI
                     DEDELKKAIEKCRIIVATKNPYPTDVHKLANDFNQPKDAPVKYKEKFIVNFRNQWLPV
                     NTKDIACFMRDTLNYIYTFSGQYYAFDFNTLEEVEEVLDPNIFYRANRQFIIHIDSIN
                     SVKPLENQKLTLKLKEPNQQFDIDVSREKAPAFKKWINR"
     gene            complement(2638..5745)
                     /locus_tag="PLO59_00290"
     CDS             complement(2638..5745)
                     /locus_tag="PLO59_00290"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HQU99555.1"
                     /translation="MIKTVAFIFLFLMQYTVVFCQDAYTLVEQKLPEAFDLLIKTKAI
                     HRVFQDSRGFFWVMSNHGLARFDGYELKYFRHNPDNNNSISDDIALVACEDKDGNIWF
                     GMISTGLCCYNYMSNSFTNYQGKNLLQGTLGSVNCLFVDSQNEVWFSMGRTGISHLNK
                     TDSTFDTFDVITAERCPYNTPYRIEFNNFVMDIKERDSENLWLGTPDGLFNLHKKTKS
                     FTVLRPKKQFNLDADIYNAKSIIVAGSKVWIGGFRSGLQCYDTLTKQWSDYDIITTDL
                     LKQPRQHMQNVVLHITPKDSDTFWIASYDQGLCIFNTTTKKCRFFSEESKYNFLPKTE
                     ALFLITDNKSNLFVLYNNQYFYLKICPNLFKQFSIPRYQHLNDGYPVIESIYEDTLRN
                     TLYATTLYSDNLISINKATGISQRVKPILNGKIFELPSINQIIPIDAQSVYLLTSKQY
                     FIYNLVNNHITIPLQPDTIKDTTNDKYSLTSLTVDQNKNLWFGTTHKGIIKLNTTTGT
                     TTFFATTENDTAEFGTKFIHLVYADVKNRIWYATGRASITAYYNQNIKKSVYINDHGL
                     PDVKSNSISSYNFISKGNFLFVSSIKGLMVFDISTETPTLIKHIRGVNGLGNTLVTNL
                     VFENDSLIWLDTYFGVVRYNLNNNKHQIINNDNGIDGKYQHVLSGAQKNIYVAASPYI
                     YEYMPELKAKDYLPVTPALTSFKVNDDQLNFEYALQNGQIIIKPEYSYFTVAFASLDF
                     ANAAQYRYSYQLENFNEKWVDAGNIRYATFSNLKGGNYILKIRSTLDNGITYSTIKSI
                     PIFIETVYYKTLWFKTVVVGAIATLLIAIYRYRMQQQRAIELLNTLTQRLEKEKSIVH
                     YENLKQQLNPHFLFNSLTSLSSLIATEPKIARQFVDQMSKVYRYILKSSDHETVLLAD
                     ELKFANTYIQLQKTRFENGFEVHITIDDRYLNRSIVPVTLQNLVENALKHNIVDDESP
                     LKIEVFVQQEYLVVKNNLQLKTVVETSNKQGLDKMKSLYKYLSCNPIVVENNDGFFQI
                     KIPLI"
     gene            complement(6494..>7082)
                     /locus_tag="PLO59_00295"
     CDS             complement(6494..>7082)
                     /locus_tag="PLO59_00295"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU99556.1"
                     /translation="PTNTTEHLLYDFNMGVGDTVKGYLESFTFPTVDIVQSIDSALVG
                     STYRKRWNINNCYNIQLIEGIGSTYGLFEPSPGCVPDLPTYSLICFQQNGQTLYPSTT
                     TNCQLIASVNYIDNNLNLINVFPNPAKGAFIIDIKNADIKEIKLSDVLGNIIHQQPAH
                     NKTQIKIDNLQSGTYILTVTDKHNRATFKKIISCP"
BASE COUNT         2245 a         1201 c         1286 g         2350 t
ORIGIN      
        1 aaaaaactat taactgccct gctgtagttt attttactac cgataaaaaa acattaccta
       61 ctgtattaag tgccgctgaa gcaggttttg ttaaagctga actagcagcc gaaaaaaaat
      121 taatagttgt aaatcaatat aacaaacatg tttttattca actaatcgaa aagaaggata
      181 aaaatgtact aatggaaagt ttgcgtaaag caggcagcca aatggctgat atgattaata
      241 aattaaaagt tacagaagtg ctgctaaccg atgcaaagtt agatgatgaa ttattggctg
      301 ttgccgaagg tatggcctta gctaattatc agtttttaaa atataagacc caagccgaaa
      361 aagaaaaaaa caaacttcaa accatttttt tacatgccga tgcattaaag caaaagccta
      421 aaaaagtaga tcagcttcaa atattggttg acagtgtttg ccgcgcacgc gatttggtta
      481 acgaacctgt aatttattta acagcaacac gcttagccga agcatttaaa aaaatggggc
      541 aagaagccgg ttttaaggtt gaaattttta ataaaacaaa aattgaaagc ttaaaaatgg
      601 gtggcttgct ggctgttaat tatggcagtg ttgaacctcc tacttttact gtaatggaat
      661 ataagccccg caatgcaaat aataaacgac cttatgtttt agtaggcaaa ggtgtggtat
      721 atgataccgg tggtttgagt ttaaaaccaa cacccaacag tatggattat atgaaatgcg
      781 atatgggcgg cagtgctgta gttggcgcaa ccttgtatgc cattgcaaaa gccaaattgc
      841 cgatacatgt tattggtttg gtacctgcta ccgataatag gccgggtggt aatgcctata
      901 ctcccggtga tgtaattacg atgatgagtg gccttaccgt tgaagtatta aataccgatg
      961 ccgaaggacg tatgatttta gctgacgctt tacactttgc aaaacaatac gaccccaaat
     1021 tagtttgtga gtttagtaca cttaccggtg ctgctgctgc tgcaattggc cagtatggca
     1081 ttgtttgcat gggtactgcc gatgagaaag taaaaaataa acttaaagaa agcggtgatg
     1141 ctgtgcatga acgtttagcc gaatttccgt tttgggatga ttacgatgaa ctgattaaat
     1201 cagacattgc cgaaattaaa aacattggcg gcccttacgg tggtgctata actgcaggta
     1261 agtttttaag ccgctttgcc gattatcctt ttatgcattt tgatattgca gggccggcat
     1321 ttataaaagg taacgacagt tatcgtggta aaaatggtac cggtgttggt gttaggctga
     1381 tgtttgattt cttaaaaaaa ctataaacac tatggtaaat tagttacccc agattagtaa
     1441 tatctgataa acaaaacgaa gtgtgtttgt tacggcataa gtaactataa acacacttcg
     1501 ttttgtttta ggtgtggttt aatcagatac tttttttttg atgctattaa taaggcaggt
     1561 ttgtattatc ttattaagaa atagccagat gagaaaacat aatcagttta aaaaatcagg
     1621 gatacttgca accataaaaa gcaaatgcaa gggttgtgca actaaagcaa atggacttga
     1681 tattgctttt tacatactta ttataatgct atgcctatat attattagca caagccttaa
     1741 cttcactaat taagtatggc atattgatgc taaatattgc aactaataga ttatactttt
     1801 atcggttaat ccatttttta aaagccggag ctttttcacg gcttacatca atatcaaatt
     1861 gctgattggg ttcttttagt ttcaaagtta gcttttgatt ttccaaaggt tttacactgt
     1921 ttatcgaatc aatatgaata ataaactggc ggttggcacg gtaaaatata tttgggtcga
     1981 gcacctcctc tacttcttct aaagtattaa agtcgaatgc atagtactgc cccgaaaatg
     2041 tataaatata gtttagcgta tcgcgcataa agcaggctat atctttggtg ttaacaggca
     2101 gccactggtt gcgaaaattt acaataaatt tttccttata ctttaccggg gcgtctttgg
     2161 gttgattaaa atcgttagcc aatttatgca catcggttgg atatggattt tttgtagcca
     2221 caattatcct gcacttttca atggcttttt ttagttcatc ctcatctatt ggtttcaaca
     2281 aataatcaac accgttaact ttaaatgcac gcatagcata ttcatcataa gctgttgtaa
     2341 aaacaacagg gcacgacagt ttaaaggttt caaaaatttc gaaactaatt ccgtcactta
     2401 attgtatgtc cataaaaatt aaatcaggct cggcattgtt cataagccaa cgctttgatg
     2461 tttttaaact gggtaaaatt tctattatct ctacatccgg tacaactgca tttattttga
     2521 attgcaactc ccttgctatt aatttttcgt cttcaattat tatcgctttc atatatttag
     2581 attgtagtgt ttaaatttta aacattaaag ctgtttgaaa tatcaggtaa taagaaatta
     2641 tataagcgga atttttattt gaaaaaaccc atcattgttt tcaaccacaa tggggttgca
     2701 gcttaaatat ttatacaatg atttcatttt atccagtcct tgtttgttcg atgtttcgac
     2761 taccgtttta agttgcaaat tgtttttaac caccaagtac tcctgttgta caaacacttc
     2821 aattttcaat ggcgactcat catctacaat attatgtttc aatgcatttt caactaaatt
     2881 ttgcagtgta accggtacaa tgcttctgtt caaataccta tcgtcaatag taatatgtac
     2941 ttcaaatccg ttttcgaaac gggttttttg taattgaatg taggtgtttg caaattttaa
     3001 ttcgtcagcc aaaagcaccg tttcgtggtc gctacttttt aaaatatagc gatacacttt
     3061 actcatctga tcaacaaact gacgggcaat tttgggctca gtagctatta aactgctaag
     3121 cgatgtaagt gaattaaata aaaagtgtgg gttaagttgt tgcttcagat tttcataatg
     3181 cacaatagac ttttcttttt cgagcctctg tgtaagtgta tttaacaatt cgatggcgcg
     3241 ttgttgctgc atacggtagc gataaattgc aataagcaat gtagcaatgg caccaaccac
     3301 aaccgtttta aaccaaagcg ttttataata aacagtttca ataaaaatgg gtattgattt
     3361 tatcgtactg taagtgatgc cattatctaa agttgaacga atttttaaaa tataattacc
     3421 accctttaaa ttagaaaaag ttgcatagcg gatattgcct gcatcaaccc acttttcatt
     3481 aaaattttct aattgataac tataacgata ctgagcagca ttggcaaaat ctaaacttgc
     3541 aaaagctacc gtaaagtaac tgtactcggg cttaataata atttggccat tttgtaaagc
     3601 atattcaaag tttaattgat catcattaac tttaaaagag gtgagagccg gagtaacagg
     3661 taaataatct ttagctttta gttcgggcat atactcataa atatatggcg atgcggctac
     3721 atatatattt ttttgagcac cacttaaaac gtgctggtat ttcccatcaa tgccattatc
     3781 attatttatt atttggtgtt tattgttgtt taaattatac ctaacaacac caaagtaagt
     3841 atcgagccaa attaaactgt cgttttcaaa aaccaaattg gtaactaatg tatttcctaa
     3901 accgtttaca cctcgtatat gtttaattaa agttggcgtt tcggtactga tgtcaaaaac
     3961 catcaaacct ttaatgctac tcacaaacaa aaaatttcct ttagatataa aattataaga
     4021 agatatactg ttacttttta catcgggcaa gccatgatca ttaatataaa cacttttttt
     4081 tatattttga ttgtagtaag cagtaattga tgcacgtccg gttgcatacc atatacggtt
     4141 tttaacatcg gcataaacca aatgaataaa cttggtacca aattctgccg tatcattttc
     4201 ggtagttgca aaaaaagttg tagtgcccgt tgttgtattt aatttaataa tgcctttatg
     4261 cgttgtacca aaccacaaat ttttattctg gtcaactgtt aggcttgtaa gactgtactt
     4321 gtcattagta gtatccttaa tggtatcagg ctgaagcggt atggtaatgt ggttattaac
     4381 cagattgtat atgaagtatt gtttcgatgt gagtaaataa acgctttgcg catctattgg
     4441 tattatttga tttatgcttg ggagttcgaa aatctttccg tttagtatgg gttttacacg
     4501 ctgcgaaatt ccggttgctt tgtttattga aattaaatta tcagaatata aagtggtagc
     4561 atataaagta tttcgcagtg tatcttcata aatactttcg ataacggggt atccgtcatt
     4621 taaatgttga tatctgggta ttgagaattg cttaaataaa ttggggcata tttttaaata
     4681 gaaatattga ttgttatata aaacaaacag atttgatttg ttatcagtaa ttaaaaacaa
     4741 agcttctgtt tttggcagaa agttgtattt actttcttca ctaaaaaacc tgcacttttt
     4801 agttgtggta ttaaaaatac ataagccttg gtcgtagctt gcaatccaaa acgtatcact
     4861 atccttgggt gtaatatgca aaaccacgtt ttgcatgtgt tgcctcggtt gctttaacaa
     4921 gtcggtagta attatatcgt aatcgctcca ttgtttggtt agcgtatcgt aacactgtaa
     4981 gccacttcta aaaccaccaa tccaaacctt gctgcctgca acaattatac ttttggcatt
     5041 gtaaatatcg gcatccagat taaactgttt tttaggacgt aacaccgtaa atgattttgt
     5101 ttttttatgc agattaaaca atccgtcagg cgtacctaac cacaggtttt cgctatctct
     5161 ttctttaatg tccattacaa aattattaaa ctcaatacgg tacggtgtat tgtacggaca
     5221 gcgttctgct gttattacat caaacgtatc gaatgtacta tcggttttgt ttaaatggct
     5281 aatgccggtg cgacccatcg aaaaccatac ttcgttttgg ctgtcaacaa ataagcaatt
     5341 aaccgaaccc aacgtaccct gcaacaaatt tttgccctgg taattggtaa acgaatttga
     5401 catatagttg tagcaacaca ggccggttga tatcatgcca aaccaaatat ttccgtcttt
     5461 atcttcgcaa gctactaatg caatatcatc gctaatgctg ttgttattat ccggattgtg
     5521 ccgaaaatat tttaactcat acccatcaaa acgtgccaat ccatgattgc tcattaccca
     5581 aaaaaaacca cgactatcct gaaaaaccct gtgtattgcc ttcgttttta tcaatagatc
     5641 aaatgcttcg ggtaattttt gttcaactaa cgtataagca tcctggcaaa acactaccgt
     5701 gtattgcatt aaaaataaaa aaataaaagc tactgttttt atcacaatcc aattgtatga
     5761 aacaaatatt gggttttaaa taaaacagat atgctatttt tttattgaag tgtatttaat
     5821 gccaaatgaa gtgtgtccct ggtaagcggt ttttaagata tattaaaaca aaccggtgct
     5881 acaactattt ttaactgttg ggtttgatgt ttgttatcgc agcacccgca aagattgccg
     5941 cgattgtcca ttcagaaaaa catgtttggg aaaagttgca caggaaaaga agttcgggct
     6001 cagttactac caggaagaat acgaacgagc atggaactga cagcaaacta aacattttaa
     6061 acgcatggta aaagttaggg ccggcacggt agagcccgtg tttggcaacc tgattaatta
     6121 ctacggcctc agaaaaataa acaccatcgg agggcaacag tgcagtgcat tgttgagcca
     6181 gaatgtggga aactggtgca catcccgcat attgcgggac tgatggctgc catagcattt
     6241 aacctgaaaa agctgatgaa attcctcacc aaaaaaacga attctaaagt tgcggcattg
     6301 cataggaaac actgcgcttt taaaaaatcg ctttttgtaa acttcattaa ctcaaaaata
     6361 agctactctg attttttaac ttgctaagta catttcaaaa actaaagtgg cttaaaacgc
     6421 atttaatgca gccgtttttc agccatcaac tccggttgtt ttttatcaga gggttgtgca
     6481 acggttaccc gtgttatggg caacttatta tttttttaaa tgtagctctg ttgtgtttat
     6541 cagttactgt taggatatat gtgccacttt gcaggttgtc aattttaatt tgtgttttat
     6601 tgtgtgcagg ttgttgatga ataatattgc ctaatacatc ggataacttt atttctttaa
     6661 tgtcagcatt tttaatatca attatgaatg cgccttttgc cgggtttggg aaaacattga
     6721 ttagatttaa attattgtca atataattta cagaagcaat aagttggcaa ttagttgtcg
     6781 tactcggata aagtgtttgt ccgttttgct gaaagcaaat taaggagtag gtaggcaggt
     6841 cgggaacaca acccggtgaa ggttcaaaca agccataggt tgatccaatg ccctcaatca
     6901 gttgaatgtt gtagcaatta ttaatgttcc acctcttgcg ataagtgctt cctactaaag
     6961 cagagtcaat ggattgtacg atatcgactg ttggaaatgt aaaagattcc agatatcctt
     7021 taaccgtatc gccaactccc atattaaagt cgtatagtaa gtgttcggta gtattagtag
     7081 ga
//
LOCUS       DAOMAA010000005         6814 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_6129_length_6814_cov_2.992307, whole genome shotgun sequence.
ACCESSION   DAOMAA010000005 DAOMAA010000000
VERSION     DAOMAA010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6814
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6129_length_6814_cov_2.992307"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..4432
                     /locus_tag="PLO59_00300"
     CDS             110..4432
                     /locus_tag="PLO59_00300"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU99557.1"
                     /translation="MLLPVSKAQVSMVDRPQAVNHQTNTMQLDGLFDVSLGLQRICVY
                     IRNDGTVPITNVTATASFNTNSGIILTDSIETFGTLMPGVAVMGFYTANFSGSYPDKH
                     LLSLQVTGTSFSQSVSRHLFVLKSINIDSSSWISYLPEGSIKTQILTDHYNGTLATLG
                     MPKDFIWTVNYKTPFTGQYGPIAFNDPWWKVAGAITGVVGGLTYVAGGIVKYCGNEKE
                     GELVKDIGTGVGGAGAIVAAADSIDVFRRGQQNTMPLPNEFTLKEEVDMHVNYAPNAG
                     IGVHNYSAQVTWNYKRFTTGNTYLYTETETVYNNIHFTTSRTTTINNTNFNTNNQIVV
                     TASVNGFQGIQNNKGYFIANMYPGNQTKLNQLVRSVVMRDDGHNGDITAADGIYTALT
                     PAAGLLTNQPMGIYILGYDRNNAPDNIDPLQAAIEIGGVLISTPNLNGCEIIADYMIM
                     VMSPININITTLAASCYNANNGSATAVAVGGTAPYTYLWSNGQTTANINNLAPGTYTV
                     TVTSANGQSGVQFTTVNQPKLLPVEATIEPPFCNTSGTGSINITVNNFTPPLSFLWFD
                     GITASSRSNLLPGAYTVTVTDGAGACFSEVYELQANTPYLMQCPTDITVHTLPGHNYA
                     IVNYDIDITNGCQNFITYNLHPPSGTWFNKGVHTVTCQAVDALGDTSQCIFTVYVNDP
                     EAPQALIKDTTLTLNTQGILTLNPALLSDSSFDNCGIVSSEIAPNILTCSNIGITPVT
                     LTVYDGNNYYWHILTNSIAYYNNGNSLINTISTTANAGVIVCRNAGFIVTDQDRVRLY
                     NSQGDLLVTIFTLGKVTVTLTGDGGFIVTDDGRVRKYDSEGNLQSTIFTTGVVQVITL
                     GDCGFIVVDEGRVRKYDNAGNLQSTIFTNGAVKVVVIPPCDFIVIDEGRLRKYDSEGN
                     LQSTIFTSGTAGVIITGDGGFIVTDDGRVRKYDSEGNLQSTIFTTGVVQVITLGDCGF
                     IVVDEGRVRKYDNAGNLQSTIFTNGAVKVVVIPPCDFIVIDEGRVRKYDSEGNLQSTI
                     FTSGTAHVIVTGDGGFIVIDDGRIRKYDSEGNLQSTIFTSGNVQVLPDNNGFKVMDDN
                     RVRTYDNQGNLTNIIITTGSQKIVVNKANQTTATTLINLLNPIHINASVQHINCNGSK
                     SGKITLTVTGNSNPYTYLWSNGKTSKNIGSLAAGTYIVTVTDKNGCTANNTYEVTQPL
                     PIVISLNKNNVKCGGKSTGKATATVTGGTAPYSFSWNTNPVSTNATASGLSAGTYIVT
                     VTDANGCTKTKSVIITENAPINVVIQPSIGHATAVVTGGVQPYTYQWNTVPVQTQATA
                     TGLICGTTYTVTVTDAKGCKKTATHIENCSRLLNDSANNTFEVITYPNPATDFIQINW
                     TGNETLSYTITLTDVLGHQLQTKITVGNQAVLNLADYDNGIYLVTINCNNATATTRIV
                     LNKE"
     gene            4714..5184
                     /locus_tag="PLO59_00305"
     CDS             4714..5184
                     /locus_tag="PLO59_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99558.1"
                     /translation="MKKILLSMLTWCILTTVQAQMYNTGIGVRIGVDNGISLKHFVSD
                     KNAVEGIVTFYKGINATALYEWQREHDFDVKGLAWYYGVGGHVGFWNKNKSPFNEEGS
                     GTKAVIGLDGVIGLEYLFENAPITVGLDVRPIVNLIGDDGLWFQAGINVRYVFN"
     gene            complement(5194..5940)
                     /locus_tag="PLO59_00310"
     CDS             complement(5194..5940)
                     /locus_tag="PLO59_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQU99559.1"
                     /translation="MIELIQVSKTFNSATPQQIAALKPINLNLPAQSFTVVVGSNGSG
                     KSTLLNAIAGSIDVKGMIKIDGQDVTALAEYERSRLVARLFQNPLTGTAADLTVIENF
                     RLAYLRTQSKKPTVGINETFVAQVASKVSMLELGLENKLHQKMGSLSGGQRQALTLVM
                     AVMAPSAILLMDEPTAALDPNAAKILMQSAQQLIEANKLTALMVTHNLKDAYTYGNCI
                     IQMHDGYMIKQLNQTEKHALKPADLYNWFM"
     gene            complement(5959..>6814)
                     /locus_tag="PLO59_00315"
     CDS             complement(5959..>6814)
                     /locus_tag="PLO59_00315"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQU99560.1"
                     /translation="TAIAQGLAFMPLALGIFLAMRVLNLPDITTDGSFTIGAAIVSWL
                     LAQAWHPVLVLLMAGCCGALAGACTGLIHIKLRIHPMLAGIIVMTGLYSVNLMIMDKP
                     NVPLIGMTGLFDFWSSSDVFLRKLLVLLIIAGVLIYFMDKFLKTDLGIAMRALGNNVN
                     MLRANGVNTNRLTILGLAIANALTALSGALLAQYQGFADINMGIGIVIVGLAAVMLGE
                     SIFNFIGLNKMTLQLLAVLVGVVLFRLLIGFALTIGLNPNYIKAVTAIFILLFVGMAQ
                     LKSKPGSV"
BASE COUNT         2173 a         1395 c         1305 g         1941 t
ORIGIN      
        1 attaaatgtg gctaaagcaa gcaccaccac aaataaaaaa ggcttctgta aaaatgatgg
       61 taaatcattt ttgtgctcat ctatattact aatcatttgt ctggtattta tgttgctgcc
      121 tgtttccaaa gcacaggtaa gtatggttga caggccacag gctgttaatc atcaaaccaa
      181 taccatgcaa ttagatggtt tgtttgatgt aagcttgggc ctgcaacgta tatgtgttta
      241 tatcagaaat gatggcaccg taccaataac caatgttacg gctacagctt cgtttaacac
      301 taacagcggt attatactta ctgatagtat cgaaacgttt ggcaccttaa tgcccggtgt
      361 ggctgtaatg ggtttttaca cggcaaactt tagcggcagt tatcccgata agcatttgct
      421 ttcattacaa gtaacgggta catcatttag ccaatcggta tcacggcatc tattcgtact
      481 aaagtccatc aatattgatt ccagcagttg gattagttat ctgcccgaag gttcaattaa
      541 aacacagatt ttaaccgacc attataatgg cacacttgct acattaggta tgccaaagga
      601 ttttatctgg actgttaatt acaaaacacc ttttacgggc caatacggac ctattgcatt
      661 taacgatccc tggtggaaag ttgcaggtgc cattaccggt gtagttggag gcttaacgta
      721 tgtggccggt ggcattgtaa aatactgcgg caacgaaaaa gaaggcgaat tggtaaaaga
      781 tatcggtacc ggtgtaggcg gtgcaggcgc tattgtagct gctgccgata gtattgatgt
      841 ttttaggcgc ggccagcaaa acaccatgcc actgcctaat gaatttacac taaaagaaga
      901 agttgatatg cacgttaact atgcacccaa tgccggtata ggcgtgcata attacagtgc
      961 acaggtaacc tggaattaca agcgctttac aacaggcaat acctatttat atactgaaac
     1021 cgaaaccgtt tataataaca tacattttac taccagccgt actacaacca taaacaacac
     1081 aaactttaat accaataacc agatagttgt aacagccagt gtcaacggtt ttcaaggcat
     1141 acaaaacaat aaaggatatt ttattgccaa catgtatccg ggcaatcaaa ccaaactaaa
     1201 ccagttggtg cgctctgtag taatgcgcga tgacgggcat aatggcgaca taactgcagc
     1261 tgacggcata tacactgcat taacacctgc agccggttta ttaaccaatc agcctatggg
     1321 catttatatt ttgggttatg acagaaacaa tgcacctgat aatatagacc cactgcaagc
     1381 tgctattgaa attggtggcg tgcttatatc aacgcccaac ctgaatggtt gcgaaattat
     1441 tgccgattat atgataatgg ttatgagccc aatcaatata aacataacaa cccttgcggc
     1501 cagttgttat aatgccaata acggcagtgc aactgcagta gctgttggtg gcacagctcc
     1561 ttatacatat ttatggagta acggacaaac tactgcaaac attaacaact tagcacctgg
     1621 tacctatacg gtaactgtaa catcggcaaa tggtcaaagc ggagttcagt ttacaacggt
     1681 taatcaacca aaacttttgc cggttgaggc caccattgag ccaccatttt gtaatacatc
     1741 aggcacagga agtataaaca ttacagtaaa caactttaca ccccccctat catttttatg
     1801 gtttgatggt attacggctt caagccgcag taatttatta cccggagcat atacagtaac
     1861 tgttactgat ggtgccggtg catgtttttc agaagtttat gagcttcagg caaacacacc
     1921 atatttaatg caatgcccca cagatattac cgtgcataca ttgccgggcc ataactatgc
     1981 aattgttaat tatgatattg atataaccaa cggttgtcaa aattttatca cttataattt
     2041 acatccacct tcaggcacat ggtttaacaa aggtgtacac actgttacat gccaggctgt
     2101 tgatgccctt ggcgatacat ctcaatgtat ttttacggtt tatgtaaacg atcccgaagc
     2161 gcctcaagca ttaattaagg atactaccct aacattaaac acacaaggca tactaacgct
     2221 aaacccagca ttactttctg acagcagctt tgacaactgc ggcattgtgt catctgaaat
     2281 agcacccaat atattaacat gcagcaatat tggcattaca cctgttacac ttactgtata
     2341 tgatggcaac aattattact ggcatatatt aaccaacagc attgcctact acaacaatgg
     2401 caatagttta ataaatacaa tcagcacaac agccaatgca ggtgttattg tttgccgcaa
     2461 cgcaggcttt attgtaaccg atcaggatcg tgtaagattg tataatagcc aaggcgattt
     2521 gcttgttacc atttttacgc ttggcaaagt aaccgtaaca cttacaggcg atggcggctt
     2581 tattgttact gatgatggac gcgtgcgcaa gtatgatagc gagggcaact tacaatcaac
     2641 catttttaca accggtgttg tacaggttat aaccttaggc gattgtggat ttattgttgt
     2701 ggacgaaggc cgcgtgcgta agtatgataa tgccggcaat ctgcaatcaa ccatatttac
     2761 taacggtgcg gtaaaagttg ttgtaatacc accttgcgat tttattgtaa ttgacgaggg
     2821 tcgtttacgc aaatatgaca gcgaaggcaa tttgcagtca accattttta catcgggcac
     2881 tgccggtgtt ataattacag gcgatggcgg ttttattgta actgatgatg gacgcgtgcg
     2941 caagtatgat agcgagggca acttacaatc aaccattttt acaaccggtg ttgtacaggt
     3001 tataacctta ggcgattgtg gatttattgt tgtggacgaa ggccgcgtgc gtaagtatga
     3061 taatgccggc aatctgcaat caaccatatt tactaacggt gcggtaaaag ttgttgtaat
     3121 accaccttgc gattttattg taattgacga gggtcgtgta cgcaaatatg acagcgaagg
     3181 caatttgcag tcaaccattt ttacatcggg tacagcccat gttatagtta caggcgatgg
     3241 cggctttatt gtaattgacg atggacgcat acgcaagtat gatagcgagg gcaacttgca
     3301 atcaaccatt tttacatccg gaaatgtgca agtattacca gacaacaacg gttttaaagt
     3361 tatggatgat aacagggtta gaacgtatga taaccaaggt aatttgacaa atatcatcat
     3421 tacaacaggc agccagaaaa tagtagtaaa taaagcaaac caaacaactg caaccacact
     3481 tattaatttg cttaatccca ttcatattaa cgcatccgtg caacatatta attgcaatgg
     3541 cagtaaatcg ggtaaaataa cgcttactgt tacaggcaat tccaatccat atacatactt
     3601 gtggagcaat ggcaaaacat caaagaatat aggatcgctt gcagccggca cctatattgt
     3661 aactgttacc gataaaaacg gatgcactgc taataatact tatgaagtta cgcaaccttt
     3721 gccaattgta atatccctaa acaaaaacaa tgtaaagtgc ggtggcaaaa gcacaggcaa
     3781 agccacagct actgttacag gtggcacggc accttacagc tttagctgga atacaaaccc
     3841 ggtaagtacc aatgcaacag ccagcggctt atcagccgga acctatatag taaccgtaac
     3901 cgatgcaaat ggctgtacta aaaccaaatc agttatcatt actgaaaatg cacctatcaa
     3961 tgtggttatt caaccttcaa ttggccatgc aacagctgtg gttaccggtg gcgtacaacc
     4021 ctatacttat caatggaata cagtacctgt gcaaactcag gccactgcaa ccggtttaat
     4081 ttgcggcact acctatacag taactgtaac tgatgcgaaa ggatgtaaaa aaacggcaac
     4141 acacattgaa aactgcagcc gattacttaa tgattcagct aataacactt ttgaagttat
     4201 aacatatccc aaccctgcaa ctgattttat acaaatcaac tggacaggca atgaaacctt
     4261 aagctataca attacattaa cagacgtatt agggcatcag ctacaaacca aaatcactgt
     4321 gggtaaccag gctgttttaa atttagccga ctatgataac ggcatttatt tagtaaccat
     4381 taattgcaat aatgcaacag ctacaacgcg tatcgtgtta aataaggaat aaacgattta
     4441 attaatttat aataacaaat gccaccaata ttggtggcat ttgttatttg gttaaagtgc
     4501 aacaaacaat tttaatcaca ccaacgggtt ttatttccaa taaaatgagt tttcaaagta
     4561 ctatgtacaa aatatttaat catgtggcat taaacacaaa ccggtaattt gttatgccat
     4621 acaactttaa tacttattaa ccgttttgat aaaaaattat ccgccagaaa aatgattttt
     4681 gcggcttaat tctatcaaat aaaaataagt taaatgaaaa agattttatt gagtatgcta
     4741 acctggtgca tactaacaac agtacaagca caaatgtata acaccggtat aggcgttcgc
     4801 attggggttg ataacggcat cagtttgaaa cactttgtaa gtgataaaaa tgcagtggaa
     4861 ggtattgtta cattttacaa aggaataaat gctactgcat tgtatgaatg gcaacgtgag
     4921 cacgattttg atgtaaaggg tttagcctgg tattacggtg ttggtggcca tgttggtttt
     4981 tggaataaaa acaaatcgcc atttaatgaa gaaggcagcg gaaccaaagc cgttatagga
     5041 ttagatggcg ttattggatt agaatatctc tttgaaaatg cacccataac cgtgggtttg
     5101 gatgtacgcc ccattgttaa tttaataggc gatgatggct tgtggtttca ggcaggcatt
     5161 aacgtacgat acgtgtttaa ttaaaacaag tttttacata aaccagttat acaaatctgc
     5221 aggcttgagg gcatgtttct cagtttgatt tagctgcttt atcatatagc cgtcatgcat
     5281 ttgtattatg caattgccat aagtatatgc atctttaagg ttatgggtaa ccataagtgc
     5341 tgttagttta tttgcttcaa ttaattgctg tgcgctttgc attaaaattt ttgcagcatt
     5401 agggtcgagg gctgctgttg gttcgtccat taataaaata gccgaaggtg ccattacagc
     5461 cattaccaag gtaagcgctt gccgctgacc gccactgagg ctgcccattt tttgatgcaa
     5521 tttgttttct aatccaagct ctaacatcga tacttttgat gcaacctgag ccacaaatgt
     5581 ttcgttaata cccaccgttg gtttcttgct ttgtgtacgc aagtatgcca atctaaaatt
     5641 ttcgattacg gttaaatcag cagcagtacc agttaacgga ttttgaaaca aacgcgctac
     5701 taaacggctt cgctcatatt cagccagggc agtaacatct tgcccatcaa tttttatcat
     5761 gccctttaca tcaattgatc cggctattgc atttagtaac gttgatttgc ctgagccatt
     5821 actgcctacc acaaccgtaa aactttgagc aggcaaattt aaatttatag gttttaatgc
     5881 cgctatttgt tggggcgttg cactgttaaa cgttttgctt acctgaataa gttcaatcat
     5941 ctttaatttc aattttactt atacactgcc gggcttgctt tttaattgtg ccataccaac
     6001 aaacagtaaa ataaaaatgg ctgtaacagc ctttatataa tttgggttta agccaatagt
     6061 taatgcaaaa ccaattaata atcgaaacaa caccacaccc accaacaccg ctaaaagttg
     6121 cagcgtcatt ttgtttaatc caataaaatt aaaaattgac tcgcctaaca taacagcagc
     6181 taaccccaca ataacaattc ctattcccat attaatatcg gcaaagccct ggtactgtgc
     6241 taacaatgcg ccacttaagg ctgttaatgc attggcaatt gccaaaccaa gtatggttag
     6301 tctgttggtg ttaaccccat tagcgcgtaa catgtttaca ttatttccta atgcacgcat
     6361 ggcaatgcct aaatcggttt tcaaaaactt atccataaaa taaatcaata ccccagcaat
     6421 aattaaaagc accaaaagct tacgcaaaaa aacatcgctg ctgctccaaa aatcaaacaa
     6481 acccgtcatt ccaattaagg gtacattggg cttgtccatt atcatcagat ttacactgta
     6541 taaccccgtc attacaataa ttccggctaa catgggatgt atgcgtagtt ttatatgaat
     6601 taaacccgtg caggcaccag ctaaagcacc gcagcatccg gccattaata aaaccaacac
     6661 cggatgccat gcctgagcca gtagccacga tacaattgct gcacctatag taaaactacc
     6721 atcggtggtt atatcgggca ggtttaaaac tcgcattgcc aaaaaaattc ccaatgccaa
     6781 tggcataaat gctaaaccct gagcaatggc cgtt
//
LOCUS       DAOMAA010000006         6088 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_7434_length_6088_cov_2.771092, whole genome shotgun sequence.
ACCESSION   DAOMAA010000006 DAOMAA010000000
VERSION     DAOMAA010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6088
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7434_length_6088_cov_2.771092"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..582)
                     /locus_tag="PLO59_00320"
     CDS             complement(145..582)
                     /locus_tag="PLO59_00320"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HQU99561.1"
                     /translation="MNIKKILVPVDDSTFALKTAEAACQLAHTLQATVCMLHVVDPAI
                     LAIGAETVVYPITEVGALLEGGNKLIADINEKLKTDVRIESHVLEGVPSEIILNQAKV
                     WQCDLIVMGTHGRTGLSHLIMGSVAENVVRHSNKPVLIIPGKS"
     gene            complement(641..1498)
                     /locus_tag="PLO59_00325"
     CDS             complement(641..1498)
                     /locus_tag="PLO59_00325"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HQU99562.1"
                     /translation="MNYYIPLSILFVALLFIVIRLFNPFKIDSFQAIVFNYLTASICA
                     FILTPQTIELQPILKLLPYCLPIGLLFICIFYILAMVTRTVGVATASVISKMSVIIPV
                     LAGLYLYNENLNTYNAIGVALALIAVYLTNSQGSNKKVTWQGALLMIVYFVGSGLVDT
                     SIKFAQVHWIDAANENIFIALLFGSAAAIGFIKLTYDFLIAKKEVALKNAIGGIVLGT
                     VNYFSIYFVIKALQYPQAQSATTFAIINIGVLLTAAFCGILFFKERYKVKNYAGFALA
                     IVAIILLSN"
     gene            complement(1498..2541)
                     /gene="ribD"
                     /locus_tag="PLO59_00330"
     CDS             complement(1498..2541)
                     /gene="ribD"
                     /locus_tag="PLO59_00330"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013072985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HQU99563.1"
                     /translation="MIQPLFMQRCLQLATLGLGHVAPNPMVGCILVCDNKIIAEGYHQ
                     QYGKAHAEVNAIANAPQNFDASKTTLYVNLEPCAHFGKTPPCAQLIINSGIKNVVIAT
                     TDPFAAVNGKGIKMLTEVGVNVTTGVLQNEALFLNRRFVTFNTKNRPYVILKWAMTAD
                     GFIARNKNSLQYELPDQISNAYAQRLSHLWRMQESAIAVGFKTAMYDNPKLNVRYHQG
                     KNPLRIVIDKQLQIPAAYHILSDNAPTVIFNNQKETTVNNLSYQKIKTQPWYDVLSYL
                     YKNQLQSVIIEGGAATHQWFIDNNLWDEARIVVSNNKMKEGLKAPILLAKANTKLDLK
                     DNKVYTYYNHTTN"
     gene            2616..3470
                     /gene="prmC"
                     /locus_tag="PLO59_00335"
     CDS             2616..3470
                     /gene="prmC"
                     /locus_tag="PLO59_00335"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HQU99564.1"
                     /translation="MKIFDLRNQLIELQKNVMMQDEAAFNADQLLKHVLKIDAIKLHQ
                     NFSSHADEIVIKTCMELNNKLIAGKPLQYVLGYAYFYGHCFKVNPSVLIPRPETEELV
                     NLIVKHYKPNRNKMQVMDIGTGSGCIAISIKKVKPSFEVTAMDISSEALQVAMHNAQV
                     IGAEINFIQDDIVNVQSNLPCFDMIVSNPPYVMFSERAEMLPNVLDNEPHLALFTPDD
                     ALFFYKHILQFAKTNLKTDGSIWFEINPLKAYELIDLMQPNYHIKLLDDMFGKHRMIF
                     GIKKNIDL"
     gene            complement(3641..>6088)
                     /gene="cphA"
                     /locus_tag="PLO59_00340"
     CDS             complement(3641..>6088)
                     /gene="cphA"
                     /locus_tag="PLO59_00340"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018942874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HQU99565.1"
                     /translation="FHRVRTGTWMGHVAEHVALEIQSMAGMDTGFGRTRSTGEYGVYN
                     VVFSYVEEKVGVYAAKAAIRICQAIVDNKPYDLEEDITKMREIREDERLGPSTGSIVE
                     EAAARGIPWVRLNRHSLVQLGYGMNQKRIQATVASTTGSIAVEIACDKEDTKNLLEAQ
                     SIPVPRGRIIYTEEELKDAIDDIRFPLVLKPINGNHGRGATINVRSWEDALTALAVAK
                     KVSRGVIVEKYITGYDHRILVINYKFEAAAKRTPAAVIGDGKQNIQQLINLVNKDPRR
                     GYGHEKVLTSIKVDESTLAILTEKNLTLESVLPLGEELWLKRTANLSTGGTSTDVTDI
                     VHPYNVFMCERIARILGLDICGIDIMTPDISQPLTAVGGAVLEVNAAPGFRMHIAPTE
                     GLARNVAAPVVDMLFPPGSNARIPIVAVTGTNGKTTTTRLIAHMCKYMGHKVGFTTTD
                     GIYIQNHMLQQGDCTGPQSAEFVLKDPTVDFAVLECARGGILRAGLGFHKCDIGIVTN
                     VAADHLGLKGINNIEEMARVKGVVVESVTPNGYAILNADDPLVASMSDHVNCKVAYFS
                     LDENNPVLRKHCERGGLAAVAENGFVTIMRGTWKMRVDKIVNIPLTFSGKAIFMIQNI
                     LPAVLTGFIRNFKIEDIKLALETFIPSPAQTPGRMNLFQFKNFNVMVDYAHNPAGFMA
                     ISKFLDRIEAKPKVGIIAGVGDRRDEDIMQLGIISAQMFDEIIIRQDKNLRGRSETEI
                     IELMMKGIHSIDNKKAVSTFKSERDAIEHAIKNAKKGAFIVICSDVVPAALEQIMDYK
                     EKEDQFEIKADDIPNQH"
BASE COUNT         1829 a         1145 c         1102 g         2012 t
ORIGIN      
        1 aaatgatttt taaagtcatt ttcggtttcg atagtaaaaa tatgcgcaat aaatttactg
       61 cctttaattc tgaatatggc ttcggccgta gcatttatcg tattataggt tgcttcaaac
      121 aacatttata aattatttac ctaactatga tttacccgga ataatgagta caggtttatt
      181 cgaatggcga accacatttt cggcaacact gcccataatt aaatggctta aaccggtacg
      241 gccatgtgta cccattacaa ttaaatcgca ttgccaaact ttagcttggt ttaaaattat
      301 ttcgctgggt acgccttcta agacatggct ttctattctt acatccgttt ttaatttttc
      361 attaatatct gcaataagtt tattgccacc ctcaagcagg gcaccaactt cggttatggg
      421 atataccact gtttcggcac ctattgctaa tatagccggg tcaactacgt gtaacataca
      481 aacggtagct tgtaacgtat gtgcaagttg gcaagctgct tcagcagttt taagtgcaaa
      541 cgtactgtca tcaacaggca ctaatatttt tttgatattc attttttttg tgtttaaagg
      601 gtttaattaa aactgtaatt cattattcta ttaaataaaa ttagttagac aataatataa
      661 ttgcaacaat ggctaatgca aagcctgcat agttttttac tttatatcgt tctttaaaaa
      721 ataaaattcc acagaaagct gcagtaagca atacgccaat attaataatt gcaaacgtag
      781 tagcgctttg tgcttgcgga tattgcaacg ctttgattac aaaataaatt gaaaagtaat
      841 ttacagtgcc taaaacaatt cctccgatag cattttttag cgccacttct ttttttgcta
      901 ttaaaaaatc ataagtaagc ttaataaaac caatagctgc agccgaacca aaaagcaatg
      961 caataaaaat gttctcgttt gctgcatcaa tccaatgtac ttgtgcaaat ttaattgacg
     1021 tatcaaccaa accactgcca acaaagtaaa caatcataag taatgcacct tgccaggtta
     1081 cttttttgtt tgagccttgg ctgttggtta aataaacggc aattaaagct aaggcaacac
     1141 ctattgcatt gtaggtgttt aggttttcat tatataagta taagccggca agtacgggta
     1201 taataacact cattttactt attaccgatg ctgttgcaac acctacggtg cgtgtaacca
     1261 ttgccaggat gtaaaatata catataaaca acaaaccaat tggcaggcaa tagggtagta
     1321 gttttaaaat gggttgtagt tcaattgttt ggggagttaa aataaaagca caaatacttg
     1381 cagtaagata attaaataca atggcttgaa atgaatcaat tttaaacggg ttgaataatc
     1441 tgattacaat aaaaagtagg gcaacaaata gtatagagag cggtatatag taattcatta
     1501 attagttgta tggttgtaat aggtgtaaac tttattatct tttaaatcca gtttggtatt
     1561 ggcttttgct aataaaatgg gtgcttttaa gccttctttc attttattat ttgaaacaac
     1621 tatgcgtgct tcgtcccaca aattattatc aataaaccat tgatgtgttg ctgctccacc
     1681 ttcgattata accgattgaa gttggttctt atataaataa cttaaaacat cataccatgg
     1741 ttgtgtttta attttttgat aagataaatt gttaactgtt gtttcttttt gattattaaa
     1801 tattacagtt ggcgcattat cggatagtat gtgataggca gccggaattt gtagttgttt
     1861 gtctataaca atccgtaacg gatttttgcc ttgatggtat ctgacattta atttcggatt
     1921 gtcatacata gctgttttaa aacctactgc aatggcgctt tcctgcatgc gccaaagatg
     1981 tgacaagcgc tgtgcatatg cattgctaat ttgatcgggt aattcgtatt gcaaactgtt
     2041 tttatttcgc gcaataaatc catcggcagt cattgcccat tttaaaataa cataaggtcg
     2101 gtttttggtg ttaaaggtta caaatcgtct gtttaaaaaa agcgcttcgt tttgtagcac
     2161 accggtagta acatttactc cgacttcggt tagcatttta atgcctttgc catttactgc
     2221 agcaaaagga tcggtggttg ctattacaac gttttttata cccgaattaa ttatcaattg
     2281 tgcacaaggg ggtgttttac caaaatgagc acagggttct aaatttacat agagcgtagt
     2341 ttttgatgca tcaaagtttt gaggtgcatt tgcaatggca tttacttcgg catgcgcttt
     2401 accgtattgt tggtgatagc cttcggcaat aattttattg tcacaaacca aaatacatcc
     2461 taccattgga ttgggtgcca catggcctaa gcctaacgtt gccaattgca aacaacgttg
     2521 cataaaaagc ggttgtatca taacgccaaa tgtaacattg ctgcttaaaa attgtagttt
     2581 actttattat tttgcgtttg aaacatgggc gacttatgaa aatatttgat ttaaggaatc
     2641 aattaatcga attgcaaaaa aatgtaatga tgcaagatga agctgcattt aatgcagatc
     2701 aattattgaa gcatgtttta aaaatcgatg caattaaatt gcatcagaat tttagcagcc
     2761 atgcagacga aatagttata aaaacttgca tggagcttaa taacaaactt attgccggta
     2821 agccactgca atatgtactg ggctatgcat atttttatgg ccattgtttt aaagttaatc
     2881 catcggtatt gatacccaga cccgaaaccg aagagttagt aaatttaatt gtaaaacatt
     2941 acaagccaaa ccgaaataaa atgcaggtaa tggatattgg aacaggtagt ggttgtatag
     3001 ccatttcaat taaaaaagta aagccatcat ttgaggtaac ggctatggat atttcatcag
     3061 aagcgctcca agttgcaatg cataatgcac aagtaattgg tgctgaaata aattttattc
     3121 aggatgatat tgtaaacgtg caatcaaatt taccttgctt tgatatgata gtttcaaatc
     3181 cgccttatgt tatgttttcg gagcgtgctg agatgttgcc caatgtttta gacaacgaac
     3241 cgcatttggc actatttact ccggatgatg ccttgttttt ttacaaacac attttgcaat
     3301 ttgccaaaac caatttgaaa actgatggaa gtatttggtt tgaaattaac ccattaaaag
     3361 cttacgagtt gattgattta atgcaaccta attaccatat aaaattattg gacgatatgt
     3421 ttggaaaaca ccggatgatt tttggcatta aaaagaatat tgatttataa actttgcttt
     3481 aaagctgctt aggtgctttg taagctattt gttttgtttg tttagatact gataattttc
     3541 agttcgaaaa tatactttat aaaagcaaag gcttcattaa aattatcgta taattttaat
     3601 gaagcctttg ctttggtttt tagccaacaa taaatttgtt ttaatgttga ttgggtatat
     3661 cgtcagcctt aatttcaaat tgatcttctt tttctttgta gtccattatt tgctctaatg
     3721 cagccggcac cacatcgctg caaataacta taaaggcacc tttttttgca tttttaatgg
     3781 catgttcaat tgcatcgcgt tctgatttga aagtggaaac tgcttttttg ttatcaatac
     3841 tatgtatgcc tttcatcatt agctcaataa tttcggtttc ggaacggccg cgtaaatttt
     3901 tatcctgtcg gattataatt tcgtcaaaca tttgtgcact aataatgcct aactgcataa
     3961 tatcttcatc acgtctatcg ccaacacctg caataatgcc cactttgggt ttggcttcaa
     4021 ttcggtctaa gaacttgctt atggccataa agcctgcagg gttatgggca tagtcaacca
     4081 taacattaaa gtttttaaac tggaataaat tcatcctgcc cggagtttgt gcaggtgatg
     4141 gtataaatgt ttccaaagcc agtttaatat cttcaatttt aaaattcctt ataaagccgg
     4201 taagtactgc aggcaaaata ttttgaatca taaaaatagc cttgcccgaa aaagttagcg
     4261 gtatgtttac aatcttatct acgcgcattt tccaggtgcc acgcattatg gtaacaaatc
     4321 cgttttcggc aacagcagcc aagccaccgc gctcgcagtg tttgcgcaat acagggttgt
     4381 tttcgtctaa actaaaataa gcaactttac aatttacgtg gtctgacatg gaagcaacca
     4441 aagggtcatc ggcattaagt atagcatatc cgttaggagt tacgctttca accaccacac
     4501 cttttacacg cgccatttct tcgatattgt ttataccttt taagcccaaa tgatcggcag
     4561 ctacattggt tacaattccg atatcacatt tatgaaaacc taatccggca cgtaatatgc
     4621 caccacgtgc acattctaaa acagcaaaat caacggtagg gtcttttaaa acaaactcag
     4681 cactttgagg gccggtgcaa tcgccttgtt gcaacatgtg gttttgtata tagatgccat
     4741 ctgttgtggt aaagccaact ttatgtccca tatatttaca catgtgtgca attaaacgcg
     4801 tagtagttgt tttaccattg gtgccggtaa cagcaacaat aggaatacgc gcattactgc
     4861 caggaggaaa caacatatcc accaccggtg cagccacatt tcttgccaaa ccctctgtgg
     4921 gtgcaatgtg cattctaaaa cctggtgctg catttacttc cagtactgca ccaccaacag
     4981 cagttaaagg ttgcgaaata tcgggagtca tgatatcaat accacaaatg tccaaaccca
     5041 gtattcgtgc aatacgttcg cacataaata cattgtaagg atgtactata tctgttacat
     5101 cggtcgaagt accacctgta cttaaattag cagtgcgttt aagccaaagc tcttcgccaa
     5161 ggggcaatac gctttcaagg gttaggtttt tttcggtaag tatggcaagt gtgctttcat
     5221 caactttaat actggtaagt actttctcat gtccgtaacc cctgcgtgga tctttattta
     5281 ccaaatttat cagttgttgg atgttttgtt tgccatcgcc aataacggca gccggtgtac
     5341 gcttggctgc ggcttcaaat ttatagttga taactagaat ccgatgatca tagcctgtaa
     5401 tatacttttc gactatgaca ccacgcgaca ctttttttgc aacagctaaa gcggttagtg
     5461 catcttccca actccttacg tttatggtag caccacggcc atgattgcca tttattggtt
     5521 taagtaccaa tggaaatcgg atatcgtcaa tagcatcttt aagttcttct tccgtataaa
     5581 ttatgcgccc gcgtggtacc ggtattgatt gtgcttcgag caggttttta gtatcttcct
     5641 tatcgcatgc aatttctacg gcaatgctac cagttgttga agctacagta gcctgtatgc
     5701 gtttttgatt cataccatag cccaattgta ctaatgagtg tctattgagc ctaacccaag
     5761 gtatgccacg tgctgcagct tcttctacaa tgcttccggt actgggtccc agtcgttcat
     5821 cttcgcgtat ttcgcgcatt ttagttatgt cttcttctaa gtcataaggt ttattatcaa
     5881 ctattgcctg acaaattctg atggcagctt tagctgcata aacaccaact ttttcttcta
     5941 cataactaaa caccacattg tacacaccat attcgcctgt actacgtgta cggccaaagc
     6001 cagtatccat gccggccata ctttgtattt ctaatgcaac atgctcggcc acatgaccca
     6061 tccaggtacc ggtacgtacc cgatgaaa
//
LOCUS       DAOMAA010000007         5955 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_7704_length_5955_cov_2.874068, whole genome shotgun sequence.
ACCESSION   DAOMAA010000007 DAOMAA010000000
VERSION     DAOMAA010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5955
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7704_length_5955_cov_2.874068"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..1507
                     /locus_tag="PLO59_00345"
     CDS             116..1507
                     /locus_tag="PLO59_00345"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU99566.1"
                     /translation="MTPMRIKFAVIIFTTFFTTCTFAQSYFSKSFGSAGMACTGKGVA
                     QAIDNALYYIGNYVNLQTSEHGMELYKLDAAGNIIWRNFIGSQTAHANRIIYTAQNKL
                     AIIGQVDDSAGNADAMVLLADTTGQIIFNKTFGQLASTEMFSGIAPDKDGGFVLSGFT
                     SNPTGPGNAVYLLRIDSVGNEIWSKIYSYNVNAVGDDISATSDGNFVFGGDKKTASID
                     YNAQVIKIDTSGTIIWNTDITYAFNSGCKNILETVNGNYLLVGEAATATSAYFDPFIT
                     LLDTAGNILWSHIVPCGNGPEAGYDVIEYLPQQYIITGFGLNQSTGNTDLMIIYLDSN
                     GNETNKQYFGNSGYDQAFNLILDNSSNSFYAAGMTLVNGEGKYFLIHQPVLTTSSVVQ
                     FEKPDITIFPNPTSHILNISKRGTFKIVNHLGQCLIDTPLQQIDVSSLPNGCYIIVAT
                     IGNQVIRQKFFKI"
     gene            complement(1517..3784)
                     /locus_tag="PLO59_00350"
     CDS             complement(1517..3784)
                     /locus_tag="PLO59_00350"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HQU99567.1"
                     /translation="MYKLICFLLCINAASAQTFFRAYVPVSENNVTFTNAFAGGLNSG
                     LLSSIDLNQDGINDVFVFDNTTDRITTYLHSGLPNNADFTYAPQYENLFPPLKKWTFL
                     VDYNCDGKPDIFTRSDSLGSIALYRNDFQPGVGLTFTLITHEIPATVAGINDKVRCSN
                     LGETYTRFADIDNDGDIDALGWPDPPTGRIAYYQNKSVEMGLPCDSFRYELVSLCWGK
                     FQLAFGSNSISAFNIFCLPPKPAPINKKPIPSPNANFKLSQADLAKTIENASVAKKDD
                     TVTSLFPFDIDADNDMDLLIGDVGSHRALLALNGGTATQDLMISQDTVFPNGPHALDV
                     HSYLNFSAIDANNDGRLDLVATANRESVNNSYHLLTDVSSTSVPNYAFAQNDFLQQTM
                     IDLGVNAYPAFFDYNGDGLLDLVSGNNYYEPQVNNTKVGLALFVNTGTLTQPQYNLIN
                     RNFADFSSIAVGINATACAPAFGDLDADGDADMIIGTESGRLFYYNNTPVGGLANFNL
                     ISPFMDSIDVGNFSMPNIVDLNNDGLLDLVVGKRNGRINYYQNFGTTTNADFNKTPTS
                     DTLGKVVLLTNANGVTGYTSPTFYKSNGLTYMLVGNEKGEVLLYDSITNNINGTWRLA
                     QTTVVTGTQGKYTAPAAADVNNDGYVDFMVGNSGGGLALYMQQNPTGIAPLQFGESDI
                     KLQPNPANDVVVIQANIFVPLQLALYNLNGNVLHRQLMQGSTKLPVKNLANGVYILSV
                     ESGSKRVHKRLVVMH"
     gene            complement(3942..4787)
                     /gene="accD"
                     /locus_tag="PLO59_00355"
     CDS             complement(3942..4787)
                     /gene="accD"
                     /locus_tag="PLO59_00355"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012781572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HQU99568.1"
                     /translation="MSWFTRIKKGITTSTKEKKETPEGLWYKCPSCKHIQPSNEHEDN
                     NYVCLKCDYHQRIGSHEYFALLFDNNLFAEINEKMTSGDPLSFVDTKKYTDRVRESIK
                     KTGLNDAVRTASGKIDGMPAMIACMDFAFIGGSMGSVVGEKIARAIDYSLQHKVPFIM
                     ISKSGGARMMEAAHSLMQMAKTSAKLALLSQAKVPYISYITDPTTGGVTASFAMLGDF
                     NVAEPNALIGFAGPRVVKETIGKDLPKGFQTSEFVLEHGFLDFIINRKEIKPRISQLL
                     RLLAN"
     gene            complement(4863..5921)
                     /locus_tag="PLO59_00360"
     CDS             complement(4863..5921)
                     /locus_tag="PLO59_00360"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HQU99569.1"
                     /translation="MNINDIKNLLGNDADSLLNHKSTTISKDQLHKPGNDFVDRIFAP
                     TNRNVNVLRNLQSIYGTGRLANTGYVSILPVDQGIEHSAGASFAPNPMFFDAENIVKL
                     AVEGGCNAVASTYGVLAMVARKYAHKIPFVVKINHNEFLSYPNKFDQIMFGTVESAYQ
                     MGAAAVGATIYFGSAESGRQIVEVARAFERAHELGMATILWCYTRNNAFKVDGVDYHT
                     SADLTGQANHLGVTIQADIIKQKLATNNGGYNAVKFGKTHKDVYDKLTSSHPIDLCRY
                     QVANCYMGRAGLINSGGESKGASDMAEAVTTAVINKRAGGQGLISGRKAFQKPFDDGI
                     AMLNAIQDVYLCKDIDLA"
BASE COUNT         1800 a         1144 c         1109 g         1902 t
ORIGIN      
        1 tcaatttagc atttggcaaa taacaaaaat catattttgc ggctatatta aatgataaaa
       61 ttgcaaaact attcaataac aaaacgttgc tttgcattta tttatattcc cagttatgac
      121 tccaatgaga ataaaatttg cggttataat ttttacaact ttttttacta cttgcacctt
      181 tgctcaatct tatttttcga aatcgttcgg tagtgcaggc atggcatgca ccggtaaggg
      241 agtagcacaa gcaattgata atgcattata ttatattggc aactatgtaa atttgcaaac
      301 gtcagaacac ggcatggagc tatacaaact cgatgctgca ggtaatatta tctggcgcaa
      361 ttttattggc tcgcaaaccg cacatgccaa ccgcattatt tatacagcac aaaacaaatt
      421 ggccattatt ggtcaggttg atgacagtgc cggcaatgcc gatgcaatgg tacttttggc
      481 agatactacc ggacaaataa tatttaataa aacttttggt caattagctt caactgaaat
      541 gttttcgggt attgcaccgg ataaggatgg tggttttgtt ttatcgggtt ttaccagtaa
      601 tccaactggg ccgggcaatg ctgtttattt gcttcgcatt gattctgtag gaaatgaaat
      661 atggagtaaa atttatagct ataacgtaaa tgccgttggc gatgatatta gcgctacttc
      721 agacggcaat tttgtttttg gaggcgataa gaaaacggca agtatagact acaatgcaca
      781 agtaattaaa attgatactt ctggtacaat aatttggaat acggatataa cctacgcttt
      841 caacagtggc tgtaaaaaca ttttagaaac ggttaatggc aattatttat tagttggcga
      901 agcggccaca gcaaccagtg cttatttcga tccgtttatt acgttgcttg atactgctgg
      961 taatatttta tggtcgcata ttgtaccttg tggcaatggt cccgaagccg gttatgacgt
     1021 tattgaatac ttaccacaac aatacataat tacaggtttt ggtttaaatc agtcaacagg
     1081 caataccgat ttaatgatta tttatttaga ctcgaacggc aatgaaacta ataaacaata
     1141 ttttggaaac agtggatatg accaggcttt caatctgatt ttagataaca gtagcaatag
     1201 tttttatgct gccggtatga cattagtaaa tggcgagggt aaatattttt taatacatca
     1261 accagtgctc actacttcaa gtgttgttca gtttgaaaaa cctgatataa ctatatttcc
     1321 aaatccgacc agtcatattt taaacatcag caaacgtgga acttttaaaa ttgtaaacca
     1381 tttggggcaa tgtttaattg acacaccatt gcaacaaatt gatgtaagca gtttgccaaa
     1441 tggttgttat ataattgtag ctaccattgg aaaccaggta atcaggcaaa agttttttaa
     1501 gatttaatgt tgcattttaa tgcataacca ctaatcgctt atgcacacgt ttactaccgg
     1561 attctacact tagtatataa actccgttag ctaaattttt aacaggtaat tttgtactgc
     1621 cttgcattag ctgccgatgc aacacatttc catttaaatt atataaggca agctgcaacg
     1681 gcacaaaaat gtttgcttga ataactacca catcattagc cgggttgggt tgtaacttta
     1741 tatccgactc gccaaactgc aaaggtgcaa tgcccgttgg attttgttgc atgtaaagcg
     1801 ccaaaccacc acctgaattg ccaaccataa aatctacata gccatcgttg tttacatcgg
     1861 ctgctgccgg tgctgtatat ttaccttgtg tgcctgtaac tacagtggtt tgtgcaaggc
     1921 gccaggttcc atttatgttg ttggttatgc tgtcatataa aagcacttcg cctttttcgt
     1981 taccaaccaa catgtaagtt aatccattgc ttttataaaa agtaggtgat gtataacctg
     2041 taacaccatt ggcattagtt aataaaacaa ctttgcctaa tgtatcgctt gttggtgttt
     2101 tattaaagtc ggcattggta gttgtaccaa aattttgata ataattaata cgcccatttc
     2161 gtttgccaac cactaaatca agcaagccat cgttgtttaa atcaacaatg tttggcatgc
     2221 taaaatttcc tacatcaata ctatccataa atggcgaaat taaattgaaa ttagctaacc
     2281 caccaaccgg tgtattgtta taataaaaca gccggccgct ttcagttcca ataatcatat
     2341 cggcatcgcc atcggcatct aaatcgccaa aagcaggtgc acaagcggtg gcatttatac
     2401 caactgcaat actgctaaag tcagcaaaat tgcggtttat aaggttatac tgtggttgtg
     2461 ttaatgtacc tgtatttaca aatagtgcta aacctacctt ggtgttattt acctgaggct
     2521 cataatagtt attgccagaa actaaatcta acaatccatc accattgtaa tcaaaaaaag
     2581 ccgggtaagc atttacaccc aaatcaatca tggtttgctg taaaaaatcg ttttgtgcaa
     2641 aagcataatt tggaacactg gtactactaa catcagtaag caaatggtag ctgttattta
     2701 cgctttcgcg attggctgtt gcaaccaaat ctaacctgcc atcattgttt gcatcaatag
     2761 ctgaaaagtt taagtatgag tgtacatcaa gggcatgagg gccattggga aaaacagtgt
     2821 cttgcgaaat cattaaatct tgtgttgctg tgccaccgtt aagcgcaagc aatgccctgt
     2881 ggcttcctac atcgccaata agcaaatcca tatcgttatc ggcatcaata tcaaaaggga
     2941 ataaactggt tacagtatca tctttttttg ctactgatgc attttcgata gttttagcta
     3001 aatcggcttg cgatagttta aagtttgcgt tgggtgatgg tattggtttt ttattgattg
     3061 gcgcaggttt aggtggcaag caaaaaatat taaatgcact tatgctatta cttccaaaag
     3121 ccaattgaaa tttgccccaa cacagcgaca ccaactcata cctgaaacta tcgcaaggaa
     3181 gacccatttc aactgattta ttttggtagt aagcaattcg accggttgga ggatcgggcc
     3241 aacccaatgc atctatatcg ccatcattat caatgtcggc aaatcgggta taggtttcgc
     3301 ctaaattact gcaccgtact ttatcattta tacctgcaac cgttgccgga atttcatggg
     3361 taattaaagt aaatgttaag cctacaccgg gttgaaaatc gtttctataa agtgcaatac
     3421 ttcccaaact atcgctacgt gtaaaaatat caggtttgcc atcacaatta taatctacta
     3481 aaaaagtcca ttttttaagt ggtggaaaca agttttcata ttggggagca tacgtaaaat
     3541 ctgcattatt gggcaagcct gaatgtaaat aggttgttat tctatcggtt gtattgtcga
     3601 atacaaatac atcgttaatg ccatcttggt ttaaatcaat agaacttaac aaaccactgt
     3661 ttaagccacc ggcaaaggca ttggtaaacg ttacattgtt ttcggatacg ggtacatagg
     3721 ctctaaagaa agtttgtgca gaagcagcat taatgcaaag caaaaagcaa attaatttat
     3781 acataatgtg gtttgtttta tcttgataaa attgaagtaa cagaagtaaa aatctataat
     3841 tcaaaccagt taatgttgtg catagtaatg gcaattttgt ttaaacgcac cgataaaact
     3901 taagccatgc aaaaattttt gactaataat tcaaagaggt attagtttgc taacaacctt
     3961 aacaactgac ttatacgtgg ttttatttct ttacgattaa tgatgaagtc aagaaatcca
     4021 tgttcgagta caaattcaga agtttgaaat ccttttggca aatctttacc aatggtttct
     4081 tttactactc tggggcctgc aaaaccaatt aaggcattgg gctcggccac attaaaatcg
     4141 cctaacatgg caaaacttgc tgtaacacca ccggtagttg ggtcggttat ataagagatg
     4201 taaggcacct ttgcctggct tagcaatgcc agtttagcgc tggttttggc catttgcatt
     4261 aaggagtgtg cggcttccat catacgcgcg ccaccacttt tgctaatcat tataaagggc
     4321 actttatgtt ggaggctata atcaattgcg cgtgcaattt tttcgccaac aacgctgccc
     4381 atcgagccac ctataaatgc aaaatccata caagcaatca tagctggcat gccgtcaatt
     4441 ttgcctgatg cagttcgaac cgcatcattg aggccggttt ttttaatgct ttcgcgaacg
     4501 cggtctgtgt atttttttgt atcaacaaat gacaatggat cgcccgatgt cattttttca
     4561 tttatctcag caaataaatt gttgtcaaac aacaatgcaa aatactcgtg cgaaccgata
     4621 cgttgatgat aatcgcactt taaacaaaca tagttgttat cctcatgttc gttgctgggt
     4681 tgaatatgtt tgcaggatgg acatttgtac caaagacctt caggtgtttc ctttttttct
     4741 ttggtggatg tagtaatccc ttttttgatg cgggtaaacc aactcatgtt taacttttta
     4801 ttgcttaaaa aaaatgtgca agctatttag tttgcatata gcataacggt tactatatat
     4861 gtttatgcca aatcaatgtc tttgcacaag taaacatcct gaatggcatt tagcatggca
     4921 ataccatcat caaatggttt ttggaacgct ttacggccac ttattaatcc ttgcccgcct
     4981 gcacgtttat taattactgc tgttgtaact gcttcggcca tatcgctggc tcctttcgac
     5041 tcgcctccac tattaataag tcctgcgcgg cccatataac agttggcaac ctgataacga
     5101 cataagtcta ttggatggct gcttgtaagt ttgtcatata catctttatg cgttttgcca
     5161 aacttaactg cattataacc cccattgttg gtggccaatt tttgtttaat aatatcggcc
     5221 tgaatggtaa cacccaggtg atttgcctga ccggttaaat cggctgaagt atggtaatca
     5281 acgccatcaa ctttaaatgc attgttacgt gtataacacc acaatattgt tgccatgcct
     5341 aattcatgtg cacgttcaaa agcacgcgct acttcaacta tctgacggcc actttcggca
     5401 ctaccaaaat atatagttgc tccaactgca gctgcaccca tttggtaggc actttctact
     5461 gtaccaaaca taatctggtc gaacttattc ggatatgata aaaattcgtt gtgattaatt
     5521 ttaactacga aaggtatttt gtgtgcgtat ttacgtgcta ccattgccaa tacgccatac
     5581 gtacttgcta ctgcattgca gccaccttct acggctaact ttacaatatt ttcggcatca
     5641 aaaaacatgg ggtttggtgc aaacgaagca ccagcactgt gttcaatgcc ttggtcaacg
     5701 ggtagtatgc ttacatagcc ggtattagct aagcggccgg taccataaat tgattgcaag
     5761 ttgcgcagta catttacatt gcggtttgta ggtgcaaaaa ttctatctac aaaatcgtta
     5821 cccggcttat gtaactggtc tttgctgatg gtagtacttt tatggtttaa taagctatcg
     5881 gcatcgttac ctaacaagtt tttaatgtcg tttatattca tcatatcaat atggagtttt
     5941 tctttataaa aatat
//
LOCUS       DAOMAA010000008         5747 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_8177_length_5747_cov_3.177442, whole genome shotgun sequence.
ACCESSION   DAOMAA010000008 DAOMAA010000000
VERSION     DAOMAA010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5747
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8177_length_5747_cov_3.177442"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1092)
                     /locus_tag="PLO59_00365"
     CDS             complement(<1..1092)
                     /locus_tag="PLO59_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HQU99570.1"
                     /translation="MKTIINPIKVYYTGYQNYTNIMAILKPFKGIRPKNEFVQQVASK
                     PYDVLDEKEAKAEAGDNQLSFYHVIKPEIDLPADVDHYAFEVYDKGKTNFINMVQQGV
                     FFQDEKPCYYIYQQTMNGRKQNGIVGGASVEDYFNNVIKKHELTRPDKEEDRRNHVRV
                     SNLNYEPVFFAYPQHAALDAIVNKVVAGKAEYDFTTDDGIGHAFWVVNDDATIASITK
                     YFAELPATYVADGHHRTAAAAGVGKERREANPNHTGNEAYNFFLAVHFPDNQLAIMDY
                     NRVVTDLNGLTDAAFIKALKQNFIVTDKGSKVYRPSKLHEFGMYLSGKWYALQAKKGT
                     YNDADPVDVLDVSILSKQILEPVLNITDLR"
     gene            complement(1067..2134)
                     /gene="serC"
                     /locus_tag="PLO59_00370"
     CDS             complement(1067..2134)
                     /gene="serC"
                     /locus_tag="PLO59_00370"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003006426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HQU99571.1"
                     /translation="MKHNFGAGPGILAPQVLQQAAQGILNFNNTGLSILEISHRSKSF
                     LQVLDETVLLVKELLNIPGNYQVLFLSGGASMQFTMVPQNFLKTKAAYVASGVWANKA
                     IAEAQLIGHTQTLASSADKNYSYIPTNYTVPADVDYLHLCSNNTIYGTQMKSLPNSTV
                     PIVCDMSSDIFSKTINVADYDLIYAGAQKNMGPAGATCVIIKNEFLSKANTHLPSMMR
                     YAIHAENGSMYNTPPCYAIYVCMLTMQWLKSIGGVAAIQKTNETKAALLYQEIDRNAL
                     FTGNVQVDARSLMSVCFSAINTDVETKFKLAAEQAGMVGLAGHRLSGGFRASIYNAMP
                     VSSVEALITCMQQFENNYKSN"
     gene            complement(2242..3132)
                     /locus_tag="PLO59_00375"
     CDS             complement(2242..3132)
                     /locus_tag="PLO59_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99572.1"
                     /translation="MKTIMAFCLTASLALGQSNPSLHINVSQIEQSTITLQAYADSIQ
                     KGSTDSIRISYNRDFINALASLLKSPNTFNYHFDSLKTISKIKSNDELLRIYTWLLPT
                     DSGRRYNYFGFLQYYDSKHKNFKCIMLNETDTFPIKKTVKAANWPSAIYYKIIETKDK
                     SKTYYTLLGWHGHNQGTTRKIIDVLTFKNNEPVFGAAIFKLTNTTQYRMLYEFNANSS
                     MLLHYDAAEKKIVFDHLAAINGFKNDKSSYGATLIYDAFNWKKGQWHYMHDIDVRNKQ
                     APANNQKTNQKKRDFYTPPK"
     gene            complement(3231..3578)
                     /locus_tag="PLO59_00380"
     CDS             complement(3231..3578)
                     /locus_tag="PLO59_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014791837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HQU99573.1"
                     /translation="MAITITDECINCGACEPECPNNAIYEGGAEWRFSDGTSIRGAFN
                     RLNGESIEADAPQAPVYMDHYYIVPDKCTECVGFHDEPQCASVCPVDCCVDDENVRES
                     QEDLLSKKQRMHA"
     gene            3671..4714
                     /locus_tag="PLO59_00385"
     CDS             3671..4714
                     /locus_tag="PLO59_00385"
                     /inference="COORDINATES: protein motif:HMM:NF017686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HQU99574.1"
                     /translation="MNITETGIALSERLKRIITDFQNKSDSQNQKFEAVLHQAEIANK
                     WFTADMSVHALQGIISMLQPASLALLPAEVKVVNPKNIGIICAGNIPLVAFHDLWCVL
                     YSGHRAVLKLSGDDTVLMTFICELLCEVNPDLKQSITIVERLKNADAIIATGSNNAFR
                     YFDYYFGKYPHIFRKNRNSVALLTGNETVTQMHALADDLFMYFGLGCRSISKVFVPEG
                     YTFDFFFESIQQYGHDLLQHNKYCNNYEYHKALYLLELIPFLTNNFLIIKEAEAWSTP
                     VSVLHYQYYNDAHKLLAHLFDNKELLQCIAVADVNEWPHASLKLHPLGTLQKPLINDY
                     ADGINTLDFLNNL"
     gene            complement(4793..>5747)
                     /locus_tag="PLO59_00390"
     CDS             complement(4793..>5747)
                     /locus_tag="PLO59_00390"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU99575.1"
                     /translation="KMTAVKHGNGRDWWLLVHPFFSNDFYEYLITPAGISGPMLLSVG
                     SCIACNNTDDQGQMVFSKDGSKLCMANTYGILDIFDFDRCTGSLTLNLNLGMPYNPLN
                     GWTTFYGCSFSADGSVLYASRFDTLWQYNLNASNVKASRLYLMGLDNAKYAIGQHMIG
                     PDNKIYIATARNSNSNDSLDYKLSVINYPDSVGYACDLRPHSIWLGGHRSRGGLPNLP
                     HYELGPLTGSLCDTLAGINDINDAKLLLYPNPANKWLMINGKWLNGLPFTLTVYDVLG
                     KTHLAQNTHTTTIDISTLTPGLYVLRLQQGDKMYYGRFVKE"
BASE COUNT         1644 a         1086 c         1093 g         1924 t
ORIGIN      
        1 acgtaggtcg gtaatattta aaaccggttc caatatttgt ttgctcaata tcgaaacgtc
       61 taaaacatca acagggtcgg catcattata ggtgcctttt tttgcttgta atgcatacca
      121 tttgccactt aagtacatgc caaactcatg tagttttgag gggcgataaa ctttactgcc
      181 tttatcagtt acaataaagt tttgctttag ggcttttata aaagctgcat ccgttaagcc
      241 atttaaatcg gttactactc tgttataatc cattatagcc aactgattat cgggaaaatg
      301 taccgctaaa aaaaagttat aagcttcgtt gcctgtatga tttgggttgg cctcacgtct
      361 ttctttacct acacctgctg cagcagcggt acggtgatgc ccatctgcta cgtaggttgc
      421 aggtaactca gcgaaatatt tagttattga ggcaatggta gcatcatcat ttaccaccca
      481 aaatgcatgg ccaatgccat cgtcagttgt aaaatcatat tccgcttttc cggctacaac
      541 tttatttaca atagcatcta atgcagcatg ttgcgggtat gcaaaaaaca caggctcata
      601 atttaaatta cttacgcgta catggttgcg tctgtcttct tccttatcgg ggcgtgttag
      661 ttcatgtttt ttaatgacgt tattaaaata atcttcaacc gatgcaccac caacaatgcc
      721 gttttgttta cggccattca tggtttgctg atatatatag tagcaaggtt tttcatcttg
      781 aaaaaacacc ccttgttgta ccatgtttat aaaattggtt ttgcctttat cataaacttc
      841 aaaagcgtag tgatctacat ctgccggtaa atcaatttcg ggtttaatta catgatagaa
      901 tgagagttgg ttatcacctg cttctgcttt tgcttctttt tcgtctaata catcgtaagg
      961 ttttgaagca acttgttgaa caaattcatt tttgggtctt atccctttaa atggttttaa
     1021 tatagccata atgtttgtgt aattttggta acctgtataa tatactttaa ttggatttat
     1081 aattgttttc aaattgttgc atgcacgtaa ttaatgcttc aacactgctt acaggcatgg
     1141 cattgtatat agatgcccta aacccacccg ataaacggtg gcctgccaaa ccaaccatgc
     1201 cggcttgttc ggcagctagt ttaaattttg tttcaacatc ggtattaata gcactaaagc
     1261 acacactcat aagtgagcgt gcatcaacct gtacattacc agtaaacaaa gcgtttctgt
     1321 ctatttcctg ataaagtaat gctgctttgg tttcgtttgt tttttgaatg gcagcaacac
     1381 caccaattga ctttaaccat tgcattgtaa gcatacaaac gtaaatagca taacaaggtg
     1441 gcgtattgta catgcttccg ttttcggcat gtatggcata gcgcatcata gatggtaaat
     1501 gcgtattggc tttacttaaa aattcgtttt taataattac acaagttgca cctgcaggcc
     1561 ccatgttttt ttgtgcgcca gcataaatta aatcataatc ggcaacatta attgttttac
     1621 taaaaatatc gctgctcata tcgcaaacta taggcactgt gctattaggc aacgatttca
     1681 tttgtgtgcc atatatggtg ttgttgctgc ataaatgcag gtaatcaaca tcggccggta
     1741 ccgtgtagtt tgtaggtatg tagctgtagt ttttatcggc actgcttgcc agggtttggg
     1801 tgtgccctat aagttgtgct tcggcaatgg ctttattggc ccaaacaccc gaagccacat
     1861 aagcagcttt tgtttttaaa aagttttgtg gcaccattgt aaactgcata ctggcaccac
     1921 cactcaaaaa taaaacctga taatttccgg gaatatttag aagttctttc acaagcaaaa
     1981 ccgtttcatc gagtacctgt aaaaacgatt tgcttcggtg cgaaatttcg agaatactta
     2041 aacctgtatt attaaaatta agtatgcctt gtgctgcttg ttgtaaaacc tgtggcgcta
     2101 atatgccggg cccggcacca aagttatgct ttatggtttg gctcattttg cttaggttga
     2161 ttctaaaatg tatccgattt caattggagc gcaaataaag ccttttacca atggtttatt
     2221 gcagttaaat ttttgttgcc gttattttgg aggtgtgtaa aagtcccgtt ttttttgatt
     2281 tgttttttga ttgttagcag gtgcctgctt atttcttaca tcaatatcgt gcatataatg
     2341 ccattgtcct tttttccagt taaaagcatc gtaaattaaa gtggctccat aagaactttt
     2401 gtcgttttta aatccattta tagcggctaa atggtcgaac acgatttttt tttctgcggc
     2461 atcataatgc aacagcatac tactgttagc attaaactcg taaagcatgc ggtactgtgt
     2521 agtattggtt agtttaaaaa tagcggcacc aaaaaccggt tcattgtttt taaaggtaag
     2581 cacatcaata atttttcggg tagtgccttg gttatgtcca tgccagccta ataaagtgta
     2641 gtaggttttt gatttatctt ttgtttctat aattttataa taaatagccg atggccaatt
     2701 tgccgctttt acagttttct ttataggaaa tgtgtcggtt tcgtttagca ttatacactt
     2761 aaagttttta tgtttcgaat catagtattg caaaaaacca aagtagttgt acctgcgtcc
     2821 cgagtctgtt ggtaataacc aggtgtatat acgcagcaat tcgtcattgc ttttaatttt
     2881 cgaaatggtt tttaacgagt cgaaatggta attaaatgta ttgggtgact taagtaaact
     2941 tgccagagca tttataaaat cacggttata agaaattcga atgctatcgg tacttccttt
     3001 ttgaatacta tcggcataag cctgtaatgt tatggtgctt tgttcgattt gtgatacatt
     3061 aatgtgaagc gatgggtttg attgaccaag agccagtgac gcggttaaac aaaaagccat
     3121 tatcgttttc attctgaata aacgcaatta tcggtagtta attttttggg atcttttaga
     3181 aataaaaaaa tccggctatg caccggattc tcatttttaa atacatgagt ttaggcatgc
     3241 atccgctgtt tcttgcttaa taaatcttcc tgcgattcgc gtacattctc atcatccaca
     3301 cagcaatcaa ccggacatac agaggcacat tgtggctcat catgaaaacc tacgcactct
     3361 gtacatttat cgggtacaat gtagtagtgg tccatataca caggcgcttg tggtgcatcg
     3421 gcttcaatac tttcgccatt taagcggtta aatgcaccgc gtatagatgt gccatcggaa
     3481 aagcgccatt cggcaccgcc ttcgtaaatg gcattattag gacattcggg ctcgcatgca
     3541 ccgcagttta tacattcatc tgtaatagta atagccataa tgatgtggtt ttataatttt
     3601 gcgacaatga taaagcattt tttcaattac agcttttatc gtttttattt agaactctta
     3661 taaacaacct atgaatatta ctgaaaccgg cattgcatta agcgagcgtt tgaaaagaat
     3721 aatcactgat tttcaaaata agtctgattc gcaaaaccaa aaatttgaag ctgtattgca
     3781 tcaagccgaa atagccaata aatggtttac tgccgatatg agtgtgcatg ctttgcaagg
     3841 cattattagc atgttgcaac ctgccagttt agcgctgttg cctgccgaag ttaaggttgt
     3901 taaccctaaa aatattggaa ttatttgtgc aggcaatatt ccgttggtag cgtttcatga
     3961 tttatggtgt gttttatata gtgggcatcg ggcagtatta aagttgtcgg gtgatgatac
     4021 ggtgttgatg acttttatat gtgaactgtt gtgtgaagtt aatcctgact taaaacaaag
     4081 tataaccatt gttgagcgtt taaaaaatgc cgatgccata attgccaccg gtagtaacaa
     4141 tgcctttcgt tattttgatt attactttgg aaagtatcca cacattttcc gaaaaaacag
     4201 aaacagtgtt gcactgctaa cgggcaacga aactgtaaca caaatgcatg cgcttgccga
     4261 tgatttattt atgtattttg gtttgggatg ccgtagtatt tcgaaagtat ttgtacccga
     4321 aggctatact tttgatttct tttttgaaag catacaacaa tatgggcacg atttgttaca
     4381 gcataataaa tattgtaata actatgaata tcataaggct ttatacctat tggagttgat
     4441 tccttttttg actaataatt ttttaatcat taaagaagcc gaggcctgga gtacacccgt
     4501 ttcggtttta cactatcagt attacaacga tgcccacaag ctactggccc atttgtttga
     4561 caataaagaa ttgttacaat gtattgccgt tgctgatgtg aatgaatggc cacatgcaag
     4621 tttaaaatta caccctttgg gtaccttgca aaaaccgctt attaatgatt atgccgatgg
     4681 tataaacacc ttagattttt taaataatct ttaattattg ttttttgcta atgggctttg
     4741 gggcttaagc aaattgtcgt ttcacatttc atctctcacc actatccact aatcactctt
     4801 tcacaaacct gccataatac attttatcac cttgctgcaa acgcagtacg tataagcccg
     4861 gtgttagtgt gctgatatct attgttgtgg tatgggtatt ttgtgctaag tgtgttttgc
     4921 ccaaaacatc atatacagta agggtaaacg gcaaaccatt taaccattta ccatttatca
     4981 ttaaccattt atttgccggg ttggggtaaa gcaatagttt ggcatcgttt atatcgttaa
     5041 taccggcaag tgtatcgcat aagctgcctg ttaaagggcc taactcataa tgcggtaaat
     5101 taggtaagcc gcctctgctg cgatgccccc ccagccaaat actatgcggg cgcaaatcgc
     5161 aggcatagcc aacactatcg gggtaattaa ttaccgatag cttgtagtct aaactatcgt
     5221 ttgaattgct attacgagct gtagcaatgt atattttatt atcaggccct atcatgtgtt
     5281 gacctatagc atacttagca ttatccaaac ccattaaata taaacgggat gcctttacat
     5341 tacttgcatt taaattgtat tgccataagg tatcgaaacg cgaggcatac aataccgagc
     5401 catcggccga aaaggagcag ccataaaaag tagtccagcc atttaatggg ttataaggca
     5461 tgcctaagtt taaatttaac gttagactgc ccgtacacct gtcgaaatcg aaaatatcta
     5521 atatgccgta ggtgtttgcc atacataatt tactgccatc tttagaaaac accatttgcc
     5581 cctggtcgtc tgtgttattg catgctatac aactaccaac cgataatagc ataggcccgg
     5641 atatgcctgc aggtgtaatt aaatattcgt aaaaatcatt ggagaaaaaa ggatgtacca
     5701 acaaccacca atcgcgccca ttgccatgtt taacggcagt catcttc
//
LOCUS       DAOMAA010000009         5703 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_8282_length_5703_cov_3.225921, whole genome shotgun sequence.
ACCESSION   DAOMAA010000009 DAOMAA010000000
VERSION     DAOMAA010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5703
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8282_length_5703_cov_3.225921"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /locus_tag="PLO59_00395"
     CDS             complement(<1..837)
                     /locus_tag="PLO59_00395"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HQU99576.1"
                     /translation="MKLLLKNHLNLRTVLLWVLVLTGSIAYGQNGEALFNENCKACHS
                     PGADKVVGPGLKDVEKRKDRAWLTKWIKNSSALIKSGDAYAVKIYEEYNKTAMPAQNL
                     SDAEIGQILDYCKDFKPPVAKAPTAPTGDEKKDESFPWFLCIGVFVLIVLAAALNRVQ
                     KGLRKVMRDKQGIPQPVELKGVDAAWSWAKGNKKLLAICLLVLAVWGSVSGWYALSKI
                     GITQGYKPEQPIKFSHKLHAGDNGIQCLYCHSGAEKSKHANIPSVNVCMNCHKYVKEG
                     PVY"
     gene            1065..1466
                     /locus_tag="PLO59_00400"
     CDS             1065..1466
                     /locus_tag="PLO59_00400"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HQU99577.1"
                     /translation="MNILKSAVLIGSILLLNLLLITHNTLAQEVVIDSKLQSLLKKSI
                     EVNELNKSMTGYRIQIYSGAKRDKAVEMKTGFEQMDNLNKAYLLYQQPNFKVRVGDFK
                     TRIEAASALEKIKGTFEIAFIVRDDVSLPQD"
     gene            complement(1543..2568)
                     /locus_tag="PLO59_00405"
     CDS             complement(1543..2568)
                     /locus_tag="PLO59_00405"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HQU99578.1"
                     /translation="MALAGLRTKYTLWLLFYIAINSLILINACSNPTNKTDVQSQKQT
                     EPTVVVPAFNFDSAYAFVNDQLNFGPRVPGSKAHKNCEIYLTQKLKSYTASVIVQNGS
                     AKTFDGNIFPIKNIIAQFNPQLGNRIILSAHWDTRPFADADSINKYKPFDGANDGASG
                     VAVLLEVARQLSIHKPQVGIDIVLFDMEDYGQEQTDNRFPQQENTWCLGSQYWAANPH
                     IPNYTAQYGILLDMVGGKDAVFPKEGTGMQFASHVVNHVWQVAQGMGYSNFINQTTNP
                     TMDDHYYVNTIAKIPCIDIVHYDIQRPGYPHYHHTHGDNISVIDKNTLGQVGQLMLKI
                     IFTQALS"
     gene            complement(2543..4018)
                     /gene="cysS"
                     /locus_tag="PLO59_00410"
     CDS             complement(2543..4018)
                     /gene="cysS"
                     /locus_tag="PLO59_00410"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HQU99579.1"
                     /translation="MEHPVYITNSLTRQKENFIPLNPPHVGLYVCGPTVYGPPHLGNA
                     RPYITFDVLRKYLTYLGYKVRYVRNITDVGHLESDADEGEDKIAKKARLEQLEPMEIV
                     KLYTDLFHLAMEQLNNDKPSIEPTASGHIIEQISMIEKIIANGLAYESNGSVYFDVTQ
                     YSKQHHYGALSGKVLDDLIAGAGEEQRVLEGQEEKRNRADFALWKKAMPEHIMRWPSP
                     WGLGFPGWHIECSAMSSKYLGETFDIHGGGMDLQFPHHEAEIAQSQGCNHVAPVKYWM
                     HNNMITIDGKKMGKSLGNFIHLEQFFTGNHPLLTQAYAPMTIRFFMLQAHYRSTLDIS
                     NQALEAAEKANQKVMNAFGTLHKLKPSSNNTANIPDIRKQITTALNDDLNTAIALSHV
                     FEMVRLINAAYAGIEQLSEQTIAEFTSIYHDFVFGVLGLRNDQTGDSSDVTGLMDLII
                     AIRTEARNKKDFATSDKIRDALAQLNIRIKDEKNGVSWIKD"
     gene            complement(4185..5105)
                     /locus_tag="PLO59_00415"
     CDS             complement(4185..5105)
                     /locus_tag="PLO59_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009196898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HQU99580.1"
                     /translation="MNLLIVGTVAFDALETPFGKTDKIVGGAATYASLAASYFARKTG
                     IVSVVGDDFPAAFIKELNQHGISTEGLQIKNGEKSFFWSGRYHNDMNTRDTLETQLNV
                     LANFNPIVPESGKNADFLLLGNLMPQVQKQVLEQMITRPKMVVMDTMNFWMDTQWDAL
                     MEVIPMVDVLSINDEEARQMSKEYSLRKAAKKILQMGIKYLIIKKGEHGALLFNKAGE
                     VFFAPALPLEEVFDPTGAGDTFAGGFIGYLARTGDVSFDNMKRAIIHGSAMASFTVEK
                     FGTQRIINLEKREIDDRVKEFFKLVSFSIS"
     gene            complement(5261..5566)
                     /locus_tag="PLO59_00420"
     CDS             complement(5261..5566)
                     /locus_tag="PLO59_00420"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HQU99581.1"
                     /translation="MFEVYILYSEKSQLYYVGQTSDLAQRLLQHNGTATNNYTSKHRP
                     WVLKAAITVADIKSALILERYIKSQKRKAFIALVIEKQNDKDFISWLFDKSSIIKQG"
BASE COUNT         1662 a         1128 c         1012 g         1901 t
ORIGIN      
        1 atatacaggt ccttctttca cgtatttatg gcaattcata caaacattta ctgatgggat
       61 attggcatgt ttgcttttct cggctcccga atggcagtac aaacattgaa taccattatc
      121 gcctgcatgc aacttatgtg aaaacttaat gggttgctcg ggcttgtaac cttgggtaat
      181 accaattttt gaaagcgcat accaaccact aacactaccc caaactgcca acacaagtaa
      241 acaaattgct aatagttttt tgtttccttt tgcccacgac catgctgcat caacaccttt
      301 aagctcaacg ggctgcggga tgccttgctt atcgcgcatt acttttcgta aacccttttg
      361 tacccggttt aatgcagcgg ctaaaacaat tagcacaaaa acgcctatgc ataaaaacca
      421 tggaaacgat tcgtcctttt tctcatcgcc tgttggtgct gttggcgctt tggcaacagg
      481 cggtttaaag tctttgcaat aatctaagat ttggccaatt tcagcatcac tcaaattttg
      541 agcgggcata gctgttttgt tgtactcttc gtatattttt acagcataag catcaccact
      601 tttaattaat gcgcttgagt ttttaatcca tttggttaac catgctctgt ctttgcgttt
      661 ttcaacgtct ttcaaacccg ggccaactac cttatcggca cctggactat gacaagcctt
      721 acaattttca ttaaaaagtg cttcaccatt ttgaccatat gcaattgagc cggttaaaac
      781 caaaacccat agtaacacag tgcgtaaatt gaggtgattc ttaagtaaca atttcataca
      841 ttaacttatt aattttcaat tacttagaag tctgtcagaa ctctgtaatt ttttaaacaa
      901 tcgcgcaaat ttaaaattta catttatgcc tcaaaaaaaa agtttgtaaa ttttaacatt
      961 tatggtaatt gatgtgccag tttcacagta tttctaattt agatgcctta ttccattact
     1021 gaaacttttc taatattgat ttcactaaaa atgaatatac cattatgaac attttaaaat
     1081 cagcagtttt aattggctca atattactgc ttaacttgtt acttataaca cataatactt
     1141 tggcgcaaga ggttgtcatc gactctaaac ttcaatcgtt gttaaaaaag agtatagaag
     1201 taaacgagct taacaaatca atgactggct accgtattca aatttattcg ggtgcaaaac
     1261 gtgataaggc tgttgaaatg aaaaccggtt tcgagcaaat ggataattta aataaagctt
     1321 atttgttgta tcaacaacct aattttaaag ttagggttgg tgattttaag acgcgtatag
     1381 aagcagcatc agcgcttgaa aagattaaag gaacctttga aatagctttt atagttagag
     1441 atgatgtgag cttgcctcaa gattaaggtt gttgccctat agtattataa gccaactatt
     1501 caattcctca aacgcatttt aggtaccgtg aattggttta aactatgaaa gcgcttgtgt
     1561 aaatattatt tttagcatta actgccctac ttgacctaaa gtatttttat caattacaga
     1621 aatgttatcg ccatgtgtat ggtgataatg tgggtaaccg ggtctttgta tgtcataatg
     1681 cacaatatca atacaaggaa ttttagcaat cgtattaacg tagtaatgat cgtccatggt
     1741 tggatttgtg gtttgattaa taaagttact atatcccatg ccttgagcca cttgccaaac
     1801 atggttaact acatgcgaag caaattgcat tccggttcct tctttcggaa aaacagcatc
     1861 cttgccgcca accatatcca gcaaaattcc atattgggct gtgtaattag gtatatgcgg
     1921 gttggcggcc cagtattgag agcctaaaca ccacgtgttt tcttgctgcg gaaaccggtt
     1981 gtcggtttgt tcctgaccat aatcttccat atcaaacaaa acaatgtcaa tacctacttg
     2041 cggcttatgg attgatagct gacgggcaac ttctaataag acagctacac cgcttgcacc
     2101 atcatttgca ccatcaaatg gtttatattt attaatacta tcagcatcgg caaaagggcg
     2161 tgtatcccaa tgagctgata gtataatacg attacccaat tgcgggttaa actgtgctat
     2221 gatatttttt ataggaaata tgttaccatc aaatgttttg gcacttccgt tttgtacaat
     2281 tacagatgct gtatatgatt ttaatttttg agtaagatat atctcacaat tcttatgagc
     2341 ttttgagccc ggtactcgtg ggccaaaatt cagttggtcg ttaacaaaag cataggccga
     2401 gtcgaaatta aaagcaggta ccactactgt tggctcagtt tgcttttgcg attgtacatc
     2461 cgttttgttt gtaggattgc tgcatgcatt aatcaatatt agagaattta tggctatgta
     2521 aaatagtagc cataatgtgt acttagtcct taatccagct aacgccattt ttttcatctt
     2581 taattctgat atttagttgt gctaatgcat ccctgatttt atccgaagtt gcgaaatctt
     2641 ttttgttgcg tgcttctgtt ctaattgcaa ttatcaaatc cattaagcct gtaacgtcac
     2701 tgctgtcgcc tgtttgatcg tttcgtaatc ctaaaacgcc aaaaacaaaa tcatgatata
     2761 ttgaggtaaa ctcagcaatc gtttgttccg ataactgctc aattcctgca taggctgcat
     2821 taattagcct aaccatttca aaaacatgtg atagggcaat tgctgtattt aaatcgtcat
     2881 ttagtgccgt ggtaatttgt ttacgtatat caggtatgtt agccgtgttg tttgatgatg
     2941 gttttagttt atggagcgta ccaaaagcgt tcataacttt ttgattggct ttttcggccg
     3001 cctctaaagc ctgattactt atgtctaacg tgcttctata atgtgcttgc aacataaaga
     3061 aacggatggt catgggtgca taagcctgtg tgagcagcgg atgattaccg gtaaaaaatt
     3121 gttctaaatg aataaaattt cccaacgatt tacccatctt tttgccatca atggttatca
     3181 tattattgtg catccaatac ttaaccggag ccacatgatt gcatccttga ctttgggcaa
     3241 tttcggcttc atgatgcggg aactgcaaat ccatgccacc gccatgtata tcaaaggttt
     3301 cgcctaaata tttactactc atggctgagc actcaatatg ccagcccggg aaacctaaac
     3361 cccatggact tggccaacgc ataatgtgtt cgggcatggc ttttttccat aatgcaaaat
     3421 cggcacgatt gcgcttttct tcttgccctt ctaaaacacg ttgctcctca ccggcacctg
     3481 caattaaatc gtccaacact ttgcccgata gtgctccata gtgatgctgc ttgctatatt
     3541 gagtcacatc aaaataaaca cttccgttgc tttcgtatgc taaaccgttg gcgattatct
     3601 tttctatcat cgaaatttgt tcgatgatgt ggcctgatgc tgttggttcg atgcttggct
     3661 tgtcgttatt aagttgctcc atagcaaggt gaaacaaatc ggtatatagt ttcacaattt
     3721 ccataggctc caattgctcg agcctggcct tttttgctat tttatcttca ccttcatcgg
     3781 catcgctttc aaggtggccc acatccgtaa tattgcgcac atatcgtacc ttatatccca
     3841 aataagtcag atattttctt agaacatcga aagtgatata gggacgtgca ttacctaaat
     3901 ggggtggccc atatacagta ggaccacata catataaacc aacatgtggt ggatttaacg
     3961 gtataaaatt ttctttttga cgtgtaagcg aattggttat atatactgga tgttccataa
     4021 aaattacttc aaaaatatat tagtagatgt aaggctttga tttgtgctgt aaagtatcag
     4081 ctgctgaaaa acatcaacaa caatatgaaa gtgaagtgga tatggccttt atcataacaa
     4141 tgcaagtata attattaatc aaaatcacat aagtgaaact actattaaga aatactaaaa
     4201 ctaaccagct taaaaaactc ttttacacgg tcatctattt cacgtttttc gagattaata
     4261 attctttgtg ttccaaattt ttcgaccgtg aacgaggcca ttgctgagcc atgaataatg
     4321 gcacgtttca tattgtcgaa agagacatca ccagtacgtg ctaaataacc tataaaccca
     4381 cctgcaaagg tatcgcccgc accggttgga tcaaatacct cttctaatgg taaggctggt
     4441 gcaaaaaaca cttcgcctgc tttgttaaac aataaagcac catgctcgcc ttttttaatt
     4501 attaagtact ttatacccat ttgaagtatt tttttggcgg ctttacgtaa tgagtattct
     4561 ttactcattt gccgcgcctc ctcatcgttt atacttaaaa catctaccat gggaattact
     4621 tccattagtg catcccattg cgtgtccatc caaaaattca tggtatccat cacaaccatt
     4681 ttggggcggg ttatcatttg ctcaagtact tgtttttgaa cttgtggcat taagttgcct
     4741 aacagtaaaa agtcagcatt tttaccagac tccggtacta tcgggttaaa attggctaat
     4801 acatttaatt gtgtttcgag ggtatctcgt gtgttcatgt cattatggta gcgcccgctc
     4861 caaaaaaatg acttttctcc atttttaatt tgtaaccctt cagtacttat tccatgttgg
     4921 tttaattctt tgatgaaagc tgccggaaaa tcgtctccta caactgaaac aatgcctgtt
     4981 ttgcgtgcaa aataacttgc ggcaagcgat gcataagtgg cagcaccacc aacaatttta
     5041 tccgttttac caaatggggt ttctaatgcg tcaaatgcta cagtacctac aattaataag
     5101 ttcatttaaa ttagtataaa tcgtttactt ttaatcgtgc gaaactaata aagtttaacg
     5161 gttaaaagca tggatattta gttgtatttg gtttaccgct atataaattt tgttgttgat
     5221 taacaataaa caaaagccat ataacaggtt ataagctaga ttaaccttgt tttattattg
     5281 atgatttatc gaacaaccag cttataaaat ctttgtcgtt ttgcttttct atcaccaacg
     5341 ctataaatgc ctttcgcttc tgacttttta tataacgctc taagattaat gcacttttaa
     5401 tgtcggctac tgttatggca gcttttaaaa cccatggccg gtgtttcgat gtatagttat
     5461 tggtggctgt tccgttatgt tgcaaaagcc gttgtgccaa atctgacgtt tgacctacat
     5521 agtagagctg cgatttctct gaatataaaa tataaacctc aaacattacg aatataaaaa
     5581 aaccccgcat tgctgcgggg ttgctccccc tcctgggctc gaaccagttc cgcagtactg
     5641 cggaacatga ttaaccttgt tttattattg atgatttatc gaacaaccag cttataaaat
     5701 ctt
//
LOCUS       DAOMAA010000010         5686 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_8322_length_5686_cov_3.326940, whole genome shotgun sequence.
ACCESSION   DAOMAA010000010 DAOMAA010000000
VERSION     DAOMAA010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5686
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8322_length_5686_cov_3.326940"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="PLO59_00425"
     CDS             <1..355
                     /locus_tag="PLO59_00425"
                     /inference="COORDINATES: protein motif:HMM:NF018766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HQU99582.1"
                     /translation="LGTVEPGFNFSGLILGFLSLYRIHHILNMYLKQTWAWFSMVMML
                     FLCGYGVYLGRFSRYNSWDIMCKPHNLFNEIYMDFRYPVHNIEAFAFSATTALLMLAC
                     YLSLVVFKTYSHDKQ"
     gene            342..707
                     /locus_tag="PLO59_00430"
     CDS             342..707
                     /locus_tag="PLO59_00430"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HQU99583.1"
                     /translation="MTSNKFSLRARINSFFYAGNGIKYLINSEPNFLVHLLASVFVIF
                     MGWYFKISSNQWLALAITMALVLVTETVNTCIELLVDWISPQHQQQAGLIKDIAAAAV
                     LISAIMAMVVAAIIFLPLL"
     gene            735..1748
                     /locus_tag="PLO59_00435"
     CDS             735..1748
                     /locus_tag="PLO59_00435"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HQU99584.1"
                     /translation="MQIKVKRYNLQFKQPFQIAHGMRYHTEALYVEVYLNNVVGYGEA
                     TFPPYLSYNRNVCIALLQNCKVKFDADTFLQNGFQTDLMLPPPANAALSMALFDLYGK
                     LIKKPIHQVLQAPLKPVATYTIGMCALNDLPQKLEQASDFKLLKIKLGGHDDKAFVKE
                     IKRITSQAFCVDANQGWTNIDTAIKMIDFLYDMGCTFVEQPLPVAMHDTQAELIVRSK
                     LPLIADESCQTVDDVKELANRFNGINIKLMKCGGLHEALQLIHAANQHNLTIMLGCMS
                     EGSVGCSAAAQLAGYAQTVDLDGPLLNSNDPFNGITYHNGALVLNHKFGIGVTPNNLY
                     KPL"
     gene            1758..4685
                     /locus_tag="PLO59_00440"
     CDS             1758..4685
                     /locus_tag="PLO59_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HQU99585.1"
                     /translation="MKTYSILFFTFLSITTYAQNPLKPTPAADRLHNSITKERLSKSS
                     LVKNVPFTNIGPSIMSGRVVDVAVSPTDPTHFYVAYASGGLWFTNNNGISFTPCFDNE
                     VVMTIGDIAVDWTNNIIYIGTGENNSSRSSYSGVGIYKSNNNGKTWQHIGLAESHHIG
                     RIVLHPTQPQTLWVAAIGHLYSTNKERGIFKTTDGGKTWQHTLYIDDTTGCIDLTADA
                     FNPNILYAAAWHRHRQAWNFVESGATSGVYKSDDGGITWKALTIAGSGFPQGAGNGRI
                     GLATSKLNSNVVYAVIDNQAARPAKKPEKGLNKDTLRNMSQAQFLNVSDSLIDAYLDK
                     NDFPQKYNATYVKQQIRDGKIKPIALVHYIEDANTALFDTEVTGAEVYKSTDAGIHWT
                     KQNLNYLDEIFYTYGYFFGQIVVSPFDDNEIYIGGVRIIKSLDGGKTFKSIDADNMHS
                     DFHAYYINPNKRNHLIIGNDGGINISYDGGKTYTKCNTPPVGQFYAVTADNAKPYNVY
                     GGLQDNGVWYGPSNYKAGYGWYDSGAYPYKFLLGGDGMQTQVDRRDNNVIYTGFQFGF
                     YYRMDKNGNNAVSIQPTIELGERPLRFNWQTPIALSHFNQDILYLGSNKLHRSFDKGN
                     TWKAISPDLTQGSKKGDVAYGTLTTISESARMYGLLYTGSDDGLIHVSADGGNTWKKI
                     IKGLPENLWVSRIVASKHADSVVYCTLNAYRFDNYAPLIYRSNNLGNTWQRIFTDLPF
                     EAVNVIREDEVNPAILYVGTDNGLYVSLNEGKTSMSFNNGLPAVAVHDLFVQADASDI
                     IVATHGRSLYKASLKFVQQLHDSVTGKPLHVFQMDGTMHSKRWGSKNLYDDIPYNTPN
                     ITIPYYSKTAGIRQIKIYNADSLLIHAFADTAVAGLNYAHFDASVTSNVSAYADKNYL
                     SKPDTDTDGKIYVVPATYKVVVSDDINQVITKWVVKERKQVNRSERNPTTPGEINRKN
                     WRR"
     gene            complement(4690..>5686)
                     /locus_tag="PLO59_00445"
     CDS             complement(4690..>5686)
                     /locus_tag="PLO59_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99586.1"
                     /translation="GAAGIGFATKNKNLWHLFAELEIAPQNFSTGKFIFSTPTGTVSS
                     GTYTKNGSSINIGATYFLTRSKTAAALNSPSAYVYAQDSRHFNKPIDSAALVQLFKRN
                     QLEFNLGLRLNAHTTPITSAGPAPEIKGVTGPAIQFGYTINYHLKHALKIEAAFSSIN
                     YKYDNIYFYDSLNMKPEFFSFEYAQTYVGLGVNYLYRYPISRSILWFGESGMQVQFLT
                     EGKHNSQIAYRIYSLDENCLINRSVTKTMVPAAKFGTGLLFATRNYNMWRIGLAYQHA
                     FNKPISEDYFFQNTSTIRQQGSTKINASGFTATIGYIYSIAPSPYAIAKHRLKNK"
BASE COUNT         1801 a         1005 c         1166 g         1714 t
ORIGIN      
        1 tttggggaca gtggagcctg ggttcaattt ttctggattg attttaggtt ttttatcgct
       61 gtatagaata catcatattt taaacatgta cctgaagcaa acgtgggcct ggttttcgat
      121 ggttatgatg ttgtttttat gtggatatgg cgtttacctg ggcaggttta gccgctataa
      181 tagctgggat attatgtgta aaccgcataa cctttttaat gaaatatata tggatttcag
      241 gtatccggtt cacaatatcg aagcgtttgc attttcggca actacagcct tgctcatgtt
      301 ggcatgttac ctaagcttag tagtttttaa aacgtattcg catgacaagc aataaattta
      361 gtttacgtgc acgcattaat agtttttttt atgccggcaa cggtattaaa tatttaataa
      421 acagcgagcc aaatttttta gtgcatttat tggcttccgt atttgtaatt tttatgggat
      481 ggtattttaa aatatcatct aatcaatggc tggctttagc tattactatg gcattggtgc
      541 ttgtaaccga aaccgtcaat acctgtattg aattattggt tgactggata tcgccacaac
      601 atcagcaaca agccggctta attaaagata tagcagcagc agcagtttta ataagtgcaa
      661 ttatggcaat ggttgttgca gcaattattt ttttaccatt gctgtaaatc aaacacaggc
      721 atgtgtaact gtatatgcaa ataaaagtaa aacgttataa cctgcaattt aaacaaccat
      781 ttcaaattgc acatggcatg cgctatcata ccgaagcgct gtatgttgaa gtatatttaa
      841 ataacgtagt aggctatggc gaagctacat ttcctccata cttgagctat aatagaaatg
      901 tttgtatagc gttattacaa aattgtaaag taaaatttga tgccgatact tttttgcaaa
      961 acgggtttca aacagattta atgttaccgc cacctgccaa tgctgcactt agtatggcgc
     1021 tttttgattt gtatggcaaa ctaattaaaa agcccataca ccaggtgtta caagcgccat
     1081 taaaaccggt tgctacctat acaataggca tgtgcgcttt aaatgacttg ccacaaaagc
     1141 ttgagcaggc atccgatttt aaattgctaa aaattaaatt aggtgggcat gatgataaag
     1201 catttgtaaa agaaataaaa cgtattacat cacaagcgtt ttgtgtggat gccaatcaag
     1261 gctggaccaa cattgatact gcaataaaga tgatagactt tttgtacgat atgggttgca
     1321 catttgtaga acaaccgctg cctgttgcca tgcatgatac gcaagctgaa ttaatagtga
     1381 gaagcaagtt gccgcttatt gccgatgagt catgtcaaac agtggatgat gtaaaagaac
     1441 ttgcaaaccg gtttaacggc attaatatta agctgatgaa atgtggcggc ttacatgaag
     1501 cattacaact gatacatgca gcaaatcaac ataatctcac aataatgtta ggctgtatga
     1561 gcgaaggcag tgtaggatgc tcggcagcag cacaattagc aggctatgca caaacggttg
     1621 atttagacgg gcctttgctt aacagtaacg acccttttaa tgggataaca tatcataacg
     1681 gggcattagt tttaaaccat aaatttggca ttggtgttac accaaataat ttatataaac
     1741 cattataatt taagtttatg aaaacatata gcattttatt ttttactttt ttaagtatta
     1801 ccacttatgc acaaaatccg ctaaagccaa caccggctgc cgacaggctg cacaatagca
     1861 ttacaaaaga aagattaagt aaaagctcat tggtaaaaaa cgttccgttt acaaacatag
     1921 ggccatcaat tatgagcggt cgtgtggttg atgttgccgt atcgccaacc gatcctacac
     1981 atttttatgt agcctatgca agtggcgggt tatggtttac caacaataat ggcatttcat
     2041 ttacaccatg ttttgataat gaagtagtga tgactattgg cgatattgct gttgactgga
     2101 ctaacaatat catctacata ggaacaggcg aaaataattc atcccgttct tcgtatagtg
     2161 gtgtaggtat ctacaaatca aataacaacg gtaaaacctg gcaacatata ggcttggccg
     2221 agtcgcatca tattggccgc attgttttgc atcctacaca accccaaact ttgtgggttg
     2281 cagctattgg ccatttatat agtaccaata aggagcgcgg tatttttaaa actactgatg
     2341 gcggtaaaac ctggcaacat actttatata ttgatgatac aaccggttgc attgatctta
     2401 ctgccgatgc ttttaatcca aacattttat atgcagcggc atggcataga cacaggcagg
     2461 cttggaattt tgttgagtcg ggtgctacat cgggcgttta taaatcggat gatgggggca
     2521 taacctggaa ggccttaacg attgccggaa gcggatttcc gcaaggtgca ggcaatggcc
     2581 gtattggctt ggcaacaagc aaactaaaca gtaatgtagt gtatgcagta attgataatc
     2641 aggcagcaag gcctgcaaaa aagcccgaaa agggtttaaa caaggataca ctacgcaaca
     2701 tgagccaggc acagttttta aatgtaagcg acagtttaat tgatgcctat ttggataaaa
     2761 atgatttccc gcaaaagtac aatgccactt atgtaaagca gcaaatacgc gatggtaaaa
     2821 taaaacctat tgccttggta cattatattg aagatgcaaa cacggcattg tttgataccg
     2881 aagtaaccgg tgccgaagtg tataaaagca ccgatgccgg aatacactgg acaaaacaaa
     2941 accttaatta tttggatgaa atattttata cctacggata ttttttcgga caaattgtgg
     3001 tttcgccatt tgacgacaat gaaatttaca taggcggtgt acgcattatt aaaagtttag
     3061 atggcggcaa aacatttaaa agtattgatg ccgataatat gcatagtgat tttcatgcat
     3121 attatatcaa cccaaataaa cgcaaccacc tgattattgg aaacgatgga ggtataaata
     3181 tttcctatga tggcggtaaa acttatacca aatgtaatac gccaccggtt ggtcagtttt
     3241 atgctgttac ggccgataat gcaaaaccat ataatgttta tggtggcttg caggataatg
     3301 gcgtttggta tggtccatca aattacaaag caggctatgg atggtacgat tcgggtgcat
     3361 acccctataa atttttatta ggtggcgatg gcatgcaaac acaggttgac aggcgcgaca
     3421 acaatgttat atacaccgga tttcaatttg gtttttatta ccgaatggat aaaaatggca
     3481 acaatgcagt ttcgatacaa cctaccatcg aactgggcga gcgaccttta aggtttaact
     3541 ggcaaacacc cattgcatta tcacacttta atcaagacat actttactta ggcagtaata
     3601 aattacatcg ttcatttgat aaaggaaata cctggaaagc aatttcgccc gacttgactc
     3661 aaggtagtaa aaaaggcgat gtagcttatg gtacacttac taccattagc gaatcggcac
     3721 gcatgtatgg tttgctttac accggcagcg atgatggttt aatacatgtg agcgcggatg
     3781 gtggcaatac atggaaaaaa atcattaaag gcctgcccga aaatttgtgg gtaagccgca
     3841 ttgttgccag caaacatgcc gatagtgttg tgtattgtac tttaaatgct tatcgtttcg
     3901 ataactatgc accattgatt tatagaagca ataatttggg caacacctgg caacgtattt
     3961 ttacggattt acctttcgaa gctgtaaatg tgatacgtga agatgaagta aatccagcta
     4021 tactgtacgt aggtaccgat aatggtttgt atgtaagttt aaatgaaggc aaaacaagta
     4081 tgagttttaa taacggtttg cccgctgtgg cagtacacga tttatttgtg caggccgatg
     4141 cgtctgacat aattgtggct acgcatggtc gcagtttata taaagcttca ttaaaatttg
     4201 tacagcaatt gcatgatagt gttacaggca aacctttgca tgtatttcaa atggatggca
     4261 caatgcacag taaacgttgg ggtagcaaaa atttatatga cgatattcct tataacacgc
     4321 ccaacattac cattccttac tacagtaaaa ccgctggcat tagacaaatt aaaatttata
     4381 atgccgatag tttgttaata catgcttttg ctgatacggc tgttgcagga ctaaactatg
     4441 cacattttga tgcaagtgta accagcaatg tttcggctta tgccgataaa aactacctgt
     4501 ccaaaccgga taccgatacc gatggaaaaa tatatgttgt gcctgctacg tataaagtgg
     4561 ttgttagcga tgacataaat caagtaataa ccaaatgggt tgttaaagag cgcaagcagg
     4621 taaaccgttc agaacgtaac cctacaacac ccggagaaat aaatcgcaaa aactggcgca
     4681 gataaatttt tacttgtttt ttaaacgatg cttagctatt gcataaggcg atggcgcaat
     4741 tgaatagatg taaccaatgg tagctgtaaa acctgatgca ttaatttttg tactgccttg
     4801 ttgtcttata gttgaagtgt tttgaaaaaa ataatcctcg gatattggtt tattaaatgc
     4861 atgttgatac gccaaaccga tgcgccacat attgtaattg cgtgtagcaa acaataaacc
     4921 ggtaccaaat tttgcagcgg gcaccatggt tttagtaaca cttctgttaa taaggcagtt
     4981 ttcatcaagt gagtaaatcc tataagcaat ttggctattg tgtttaccct ctgttaaaaa
     5041 ctgtacttgc atacctgatt cgccaaacca aagtatgctg cgtgatattg gataacgata
     5101 aagatagtta acacctaaac cgacatacgt ttgtgcatac tcgaatgaaa aaaattcggg
     5161 cttcatattt aatgaatcat aaaaataaat gttgtcatat ttataattaa ttgatgagaa
     5221 tgcagcttct attttaagtg catgttttaa atgatagttg atagtataac caaactgtat
     5281 tgcaggacca gtaacgcctt ttatttcagg agccgggcca gcagatgtaa tgggtgtagt
     5341 atgtgcattt aatcgcaagc ccaaattaaa ctcaagctga tttcgtttaa atagctgaac
     5401 caatgccgca ctgtcaatag gtttattaaa gtgacggctg tcttgtgcat aaacataagc
     5461 cgaggggctg tttaatgctg cagcagtttt acttcgggtt aaaaaataag tggcacctat
     5521 atttatactg cttccatttt tagtgtatgt gcctgatgat acagttcctg ttggtgtgct
     5581 aaaaataaat ttaccggtac taaaattttg tggagctatt tctaattcgg caaacaaatg
     5641 ccacaagttt ttatttttgg tagcaaagcc aatgccggct gcacct
//
LOCUS       DAOMAA010000011         5161 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_9816_length_5161_cov_3.337054, whole genome shotgun sequence.
ACCESSION   DAOMAA010000011 DAOMAA010000000
VERSION     DAOMAA010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5161
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9816_length_5161_cov_3.337054"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="PLO59_00450"
     CDS             complement(<1..355)
                     /locus_tag="PLO59_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HQU99587.1"
                     /translation="MSKPVLSKSTFVRSLQCLKSLYLYKNYFKQRDKITPAQQAIFSR
                     GNQIGVLAQQLFAGGNNASTGTKVRNKEALLNTMEAIHQGQKIIYEASFQFEEVWVIA
                     DIIVLKDDGWHIYEVK"
     gene            complement(474..932)
                     /locus_tag="PLO59_00455"
     CDS             complement(474..932)
                     /locus_tag="PLO59_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008610891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HQU99588.1"
                     /translation="MKATGNTVKEILANVPEDRMEAFNKLHKTIVKNLPKGFEPAISY
                     GGLGYVVPHKIYPDGYHCKPVEPLPFAGIASQKNTINFYHMGIYADAELLNWFVAEYP
                     KHCKQKLDMGKSCIRFKKFDEIPFTLIGELMKKMTVANWINIYESKYKKK"
     gene            complement(975..1355)
                     /locus_tag="PLO59_00460"
     CDS             complement(975..1355)
                     /locus_tag="PLO59_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HQU99589.1"
                     /translation="MPKTDYQNVEEYHQSFSGETLSRLEIIKSIINQVAPDTVAVISY
                     QIPAYKLGAKHYLIYYAAFKNHITISSPWSTALLKHFETELSALKVSKSAIQFPNNKP
                     LPVALIQNIIRFRKAECLSVETKK"
     gene            complement(1549..4029)
                     /gene="lon"
                     /locus_tag="PLO59_00465"
     CDS             complement(1549..4029)
                     /gene="lon"
                     /locus_tag="PLO59_00465"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HQU99590.1"
                     /translation="MNFDNDNIKIKMAALADDESEMIPLLTQEDEDRMSDEKIPEILP
                     ILPLRNTVLFPGVVIPITVGRDKSIKLIRDAYKADKTIGVVSQKDVSTEDPRFDDLNK
                     IGTVAHVLKMLQMPDGNTTVIIQGKKRIELQDLQQEEPYIKASVKPLHDVKPAKNDKE
                     FKATVDSLKDISLQIIQLNQQIPAEAALAIKNIDSPSFLIHFISSNMNVESANKQEIL
                     ELVNLKERAIKVLEHLTKELQMLELKNQIQSKVKVDLDKQQRDYFLNQQLKQIQEELG
                     GNTPDQEIAEMREKGRKKSWSKEIEEHFNKEIERLQRLNPAAAEYSVTMSYLDTLLSL
                     PWNEYSEDNFDLKRAQKILDSDHYGLEKVKQRIMEYLAVLKLKRNMKSPIICLYGPPG
                     VGKTSLGKSIAKALNRQYVRMSLGGVHDEAEIRGHRKTYIGAMPGRVIQSIKKAKTSN
                     PVFVLDEIDKLSNTFHGDPSSALLEALDPEQNAAFYDNYLELGYDLSQVLFIATANSL
                     SSIQPALLDRMEIIDISGYTVEEKVQIASQYLIPKQLEAHGINKKQLSIAPSVIESII
                     EEYTSESGVRNIEKQIASIARQIAKRVAMDEKYDTQINTEEVYKILGAPRFLRDRYQT
                     NDVAGVVTGLAWTSVGGQILFIETSLSKGKGRLTLTGNLGEVMKESAHLALAYLKAHA
                     ENLKINYDVFDNWDIHIHVPEGATPKDGPSAGITMLTSLASAFTQRKVKKNLAMTGEI
                     TLRGKVLPVGGIKEKILAAKRAGIKDIILSADNLKDIEEIKPTYLKDLRFHYVKTMAE
                     VVEIALMKEKVKPALNFSIKTEITKALN"
     gene            4170..4550
                     /locus_tag="PLO59_00470"
     CDS             4170..4550
                     /locus_tag="PLO59_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99591.1"
                     /translation="MQNPFIRFILTTIKGGVFLLLPVFAIIYGVLKVADTIRPITEPI
                     ILYFGFELTFGPFTQQIFALLILFLLCFIAGLLLHLKFFKTLNIKIDSFINQLIPSYG
                     AMKSKVDNELKRFNSDDNKNANQT"
     gene            complement(4547..>5161)
                     /locus_tag="PLO59_00475"
     CDS             complement(4547..>5161)
                     /locus_tag="PLO59_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008615981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HQU99592.1"
                     /translation="TGPIYIGKNALVMEGCRIRGPFSLGDNAQLKMGTKIYGATTVGN
                     YCKVGGEVNNSIFMANSNKAHDGYIGNAVIGQWCNLGADTNCSNLKNNYSNIKVFDYL
                     EQNYIDTGLQFCGLTMGDHSKCAINTQFNTGTVVGVAANIFTQGFPPKHIPSFTWGQP
                     GNGEVFRIEDAVELAQRVYQRRGLNFDTDEAAILKHVFSLTNCD"
BASE COUNT         1402 a          958 c          907 g         1894 t
ORIGIN      
        1 tttttacttc gtaaatgtgc catccgtcat cttttagcac aataatatca gcaataaccc
       61 aaacttcttc aaattgaaac gatgcttcgt atattatttt ttgaccttga tgtatagcct
      121 ccatagtatt tagcaaggcc tctttgtttc ttacttttgt accggtgctt gcattatttc
      181 cacctgcaaa caattgttgt gctaatacgc caatttgatt gcctcttgaa aatatggctt
      241 gctgtgcagg ggtaatttta tcacgttgtt taaaatagtt tttatacagg tataacgatt
      301 ttaaacattg taaacttcgg acaaaggttg atttgcttaa tacaggtttc gacatattgc
      361 ctgcaagtat aaaattagat gtgttgtatt ggacatttac aagtagcttt cgatcaaaac
      421 aattggctcc ccaattacta aaactgcata gctgcaagct ttgagtttat attttatttt
      481 ttcttgtatt tactttcata tatatttatc caattggcaa cggtcatttt tttcatcaac
      541 tcaccaatta gtgtaaaggg tatttcgtca aactttttaa agcggataca acttttgccc
      601 atatctaatt tttgcttgca atgttttgga tattcagcaa caaaccaatt taataattca
      661 gcatcggcat aaatgcccat gtggtaaaaa ttgatggtgt ttttttgtga tgcaatgcca
      721 gcaaaaggta atggttcaac cggtttgcaa tgataaccat caggataaat tttatggggc
      781 actacgtaac ctaagccgcc ataactaatt gcaggttcaa atcctttagg caaatttttt
      841 acaatggttt tatgcaattt attaaacgct tccattctgt cttcgggcac attggctaaa
      901 atttctttta ctgtatttcc tgttgctttc atgtgttaag tattgagatt attttttact
      961 actgaaaatt aaatttattt tttggtttca actgacaaac attcggcttt tctgaatcgg
     1021 attatgtttt gaataagtgc aaccggcaaa ggtttgttat ttggaaattg aatggccgat
     1081 ttagaaactt ttaaggcgct taattcggtt tcaaaatgtt ttaacaatgc tgtactccaa
     1141 ggacttgaaa tagtgatatg atttttgaaa gctgcataat atattaagta atgtttggcg
     1201 cctaatttat atgccggtat ctggtaacta attacggcta cggtatcggg tgcaacttga
     1261 tttatgattg atttaattat ttccaagcgg cttaatgttt cgcccgaaaa tgattgatga
     1321 tattcttcaa cattttgata atcggtcttt ggcatcgtat aatttattga ttgcttaaaa
     1381 gtaacaatcg tttccgttta aaattagaat ctaatattta atagccctca tctgtttaca
     1441 aaatatattt aatagtgtgt aaataaaaaa gccataagta ttgatagctt atggctttgc
     1501 tttaaaaata aagcatagag tacaactttg cttaatgctt actgcacttt aatttaacgc
     1561 ttttgttatt tctgttttga tactaaaatt taaagcgggt tttacttttt ctttcatcaa
     1621 tgcaatttcc acaacttcgg ccatggtttt tacataatga aaacgcaggt cttttaagta
     1681 agtgggtttt atttcttcaa tatcttttaa gttatcggca cttaaaatta tatccttaat
     1741 gcctgctctt tttgctgcca atattttttc tttaataccg ccaaccggta atactttacc
     1801 acgcagggta atttcgcccg tcatggctaa attcttttta acctttcgtt gtgtaaatgc
     1861 cgatgccaaa ctggtaagca tggttatacc tgcactgggg ccatctttag gtgtggcacc
     1921 ttcgggcacg tgtatgtgta tgtcccagtt gtcaaacaca tcataattta tttttaaatt
     1981 ttcggcatgt gcttttaaat aggctagtgc aaggtgtgca ctttctttca taacctcgcc
     2041 caaattgcct gttaatgtta gcctgccctt gcctttgctt aggcttgttt cgataaaaag
     2101 aatttgacca cccacactgg tccaggccaa gccggtaact actccggcca catcattggt
     2161 ttggtaacgg tcgcgtaaga agcgtggtgc acctaatatt ttatatactt cttcagtatt
     2221 aatttgtgtg tcatactttt catccattgc cacgcgcttt gctatttggc gggcaattga
     2281 agctatttgc ttttcgatat tacgcacacc gctttcgctg gtatattctt caattattga
     2341 ttcgataact gagggtgcta tactcagttg ttttttatta ataccatgtg cttctaattg
     2401 cttgggtatc agatattgtg aagctatttg tactttttct tctaccgtgt aacccgatat
     2461 gtcaataatt tccatacggt cgagtagggc aggttgtatg gaagataatg agttagctgt
     2521 tgcaataaaa agtacctggc ttaaatcgta gcctaactct aaataattat catagaaagc
     2581 tgcattttgt tcggggtcca atgcttcgag taatgccgat gatgggtcgc catgaaaggt
     2641 attgcttagt ttatcaattt cgtctaatac aaaaacaggg ttcgatgttt tggctttttt
     2701 tatactttgt atcacacgtc caggcatagc gccaatatag gttttgcggt ggccgcgtat
     2761 ttcggcttca tcatgaacgc cacctaaact catgcgcacg tattggcgat ttaaagcttt
     2821 ggcaatactt ttacctaacg atgttttacc aactccgggt gggccgtaca aacaaataat
     2881 gggcgatttc atattgcgtt taagttttag tacagccaga tattccatta tgcgttgctt
     2941 cactttttct aagccgtaat ggtcgctgtc taaaattttt tgtgctcttt ttaaatcaaa
     3001 attatcttca ctgtactcat tccagggcag gctaagcaag gtatccaggt aactcatggt
     3061 tacactgtat tcagcagcag ctgggtttag cctttgtaac ctttctattt ctttattaaa
     3121 atgttcttct atttctttac tccaactttt tttgcggccc ttttcgcgca tttcggcaat
     3181 ttcctggtcg ggtgtattgc cacctaattc ttcttgtatt tgtttaagtt gttgatttaa
     3241 aaaataatca cgctgttgtt tatctaaatc aactttaact tttgattgta tttgattttt
     3301 taattcaagc atttgtaact ctttggttaa atgctctaac acctttatgg cacgttcttt
     3361 taaatttaca agctctaaaa tctcctgttt attagccgac tccacattca tatttgatga
     3421 tataaaatga atgaggaatg atgggctgtc aatatttttt atagccaaag cagcctcggc
     3481 cggtatttgc tggttaagtt gaatgatttg cagcgatata tcttttagcg aatcaacggt
     3541 tgccttaaat tctttgtcgt ttttggccgg ctttacatcg tgcaatggtt ttaccgatgc
     3601 cttaatgtat ggttcttctt gttgcaggtc ttgaagctcg attcgttttt tcccctgtat
     3661 aattacggtg gtgttgccat cgggcatttg cagcatcttt aaaacatgtg ctacggtgcc
     3721 aattttgttt aagtcatcaa accgtggatc ttcagtgctt acatcttttt gtgaaacaac
     3781 gcctattgtt ttatcagctt tgtaagcatc gcgaataagt ttgattgact tatcgcgtcc
     3841 aactgtaatt ggaataacca cacccggaaa caatacggtg ttacgtaatg gcaatatcgg
     3901 taatatttca ggtatttttt catcactcat tctgtcctca tcttcttggg taagcaatgg
     3961 aatcatttct gactcgtcat cggccaaagc ggccattttt atctttatgt tgtcgttatc
     4021 gaagttcata ttatttaata ttcggtcaat ttgtcaagta cagtattgtt ttggctgttt
     4081 agaaacgtaa attttaataa acggccttta gtctaacatt atgccaaggc gaagcattac
     4141 ttttttaata ttgcttaaaa cttttaacca tgcaaaatcc atttattcgt tttatactaa
     4201 cgactattaa gggtggtgtt tttttactgc ttcctgtatt tgcaataatt tatggagttt
     4261 taaaagtagc agatacgata aggccaataa ccgaacccat aattttatat tttggttttg
     4321 agctaacatt tggtccgttt acacaacaaa tttttgcctt actgatattg tttctcttat
     4381 gttttattgc cgggttgttg ctgcatttaa agtttttcaa aacattaaac attaaaatag
     4441 acagcttcat aaaccagctc atcccatcat atggcgctat gaaaagcaag gttgataatg
     4501 agctaaagcg ttttaatagc gatgataata aaaacgcaaa ccaaacttaa tcacaattgg
     4561 ttaggctaaa aacgtgtttc aagatagcgg cttcatcagt atcaaaattt aatcccctac
     4621 gttgatacac acgttgtgca agttcaactg catcttcgat acgaaatact tcgccattac
     4681 cgggctggcc ccatgtaaag ctgggtatgt gtttgggcgg aaacccctgt gtaaaaatgt
     4741 tggcggctac gcctactaca gtacctgtat taaactgtgt gttaatagca catttactgt
     4801 gatcgcccat ggttaatcca caaaattgca aaccggtatc aatgtaattt tgttcgaggt
     4861 aatcaaacac cttaatgttg ctataattat tttttaaatt gctgcaattg gtatcagcac
     4921 ccaaattgca ccattgtcca ataacagcat tgcctatata tccatcatgt gccttattac
     4981 tatttgccat aaaaatgctg ttgtttactt cgccacccac tttgcaataa ttgccaaccg
     5041 tagttgcgcc ataaattttg gttcccattt taagttgtgc attatcgcct aaagaaaatg
     5101 gacctctaat cctacatcct tccattacca aagcattttt gccaatataa atggggccgg
     5161 t
//
LOCUS       DAOMAA010000012         5137 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_9892_length_5137_cov_3.199331, whole genome shotgun sequence.
ACCESSION   DAOMAA010000012 DAOMAA010000000
VERSION     DAOMAA010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5137
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9892_length_5137_cov_3.199331"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="PLO59_00480"
     CDS             complement(<1..790)
                     /locus_tag="PLO59_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99593.1"
                     /translation="MNSYNELIQKLDAFIRKYYKNQLLRGVIYAFTAFLVFGITVTTL
                     EYIGHFDTTMRAILFYSFILVNVYFLVRYVCIPLLKLNKLGSIISHQQAAQIIGKHFT
                     NVSDKLLNTLQLQQQLQQQSSALLHASIDQKVAELRPVPFTAAIDLSANKKYLRYAAL
                     PVLTLIVILFAAPSLLRDGSQRLIKHNTFFEKPAPFTFVLENKKLQTTQASDYTLNLK
                     LAGHEIPEQVYIEIGKNTFRLDKLNVLQFSYTFRNLQKSTTFRFM"
     gene            complement(982..1443)
                     /locus_tag="PLO59_00485"
     CDS             complement(982..1443)
                     /locus_tag="PLO59_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99594.1"
                     /translation="MKNLISSALLLSMVAVTFTGCKKDDDESNTDKLTDKNWKMVALT
                     VDPPVNVGGTLISDVYAQIPACSQDDLSIFKDNNTVNFDEGATKCDPSDPQTTNGTWA
                     FNTDETVLSITESGTVTSYTIKELSGSDFKATFTDNSSGVVETYTVTFKAQ"
     gene            complement(1545..2420)
                     /locus_tag="PLO59_00490"
     CDS             complement(1545..2420)
                     /locus_tag="PLO59_00490"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010603248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQU99595.1"
                     /translation="MQLLTPTNFSQYQLIDSGNFEKLERWGSYITCRPEPQAIWDKRL
                     SNAEWQKQMHVKFVSKSSSSGIWQNVKNMPQRWQMSYDIPQGPTLQFKLALTSFKHVG
                     LFPEQAVNWDFIYHSVKQFKTPAPKVLNLFAYTGASSLAACAAGADVTHLDSIKQVVT
                     WARENMELSNLNNIRWMVEDAVTYAKREVKRGKKYNGIILDPPAFGHGPNGESWKLED
                     QINQLLKLVAQLLDDNERFLVLNTYSLGLSSLIINNMMADTFKTKNAVNTGEIFLQSS
                     TQQQLPLGVFGRFCE"
     gene            complement(2448..3470)
                     /locus_tag="PLO59_00495"
     CDS             complement(2448..3470)
                     /locus_tag="PLO59_00495"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HQU99596.1"
                     /translation="MSNNMIFVMGTSRSGTTMMGRIFGKNEAVFTFEEIHFFERIWSP
                     GSPQQHYTQTQAVEVFTKLISNQRDGYLREKHPEKYIAEAEKAIANYLAQSTNNALFA
                     PNLFAYFLQYESKLHHKSMGCDQTPLNLLFASDIFSYFPTAKAIIMVRDPRDVLLSQK
                     GKWKRKFLGAGKNIPWKETIRAYLNYHPYTISKLWNSSNNKVQKLVSHPQVMVVKFED
                     LIENPETLLKKACAFLDITYSADMLSVPQVGSSTGSDNPQALGINKSNKNKFLKGLNH
                     AEIRICESVTKNLRSAYGYTNSGVSQNYILLIWYVLSFPVKLFLAFLFNVGRIKNLRQ
                     SIKRRL"
     gene            complement(3495..4484)
                     /locus_tag="PLO59_00500"
     CDS             complement(3495..4484)
                     /locus_tag="PLO59_00500"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HQU99597.1"
                     /translation="MIGQINKEYIQKRPTKFFSRMVSYALFEGRPVTTPGQWINPLVF
                     KILNFAQQRNSNQIKEPVFILGTGRSGTTILGIVLSMHQDIAFLNEPKALWHSVFPDE
                     DLIGSYSRSNASYYLDPAYATPERIQRIHNIYGFYLYTVMSKRVVDKYPELIFRIDFL
                     KRIFPDFKALFIYRNGWDTCLSSANWSEVKGEQVKGETHDWWGVNNRKWHLLVDELVP
                     QDEDLKMHYNEIKAFTNQVDMSIVEWIISMKKGLEVMAKYPQHIMPVRYEDLVSQPVN
                     TLDTIRSFCHLQPDAALNAYALQTLKPVPAKKPIALNPILEKPFLALMQQLNY"
     gene            complement(4527..>5137)
                     /locus_tag="PLO59_00505"
     CDS             complement(4527..>5137)
                     /locus_tag="PLO59_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99598.1"
                     /translation="AEIAASYCQIVISFFNRKREVFQLPESLTLEQYFDDCKNYSEAD
                     LLREENHLYTGFEFGYYIKHIDAWYQVFGDNLKVMFFDDLRDSKKVVLSLCDWLGVDP
                     GFYANYNFEVKNKSVNYKNRFMHSLAVRLSFGAKRFWRNNQQLKSFLRSIYYKINGQP
                     VSKTDHDAEIKNRINEMYAPYNKQLAAYLKTKGFTQFPGWLK"
BASE COUNT         1480 a          944 c         1029 g         1684 t
ORIGIN      
        1 ccataaacct gaatgtggtt gacttttgca ggttgcggaa tgtgtaacta aactgaagta
       61 cgtttagttt atctaacctg aaagtgtttt tgcctatttc gatatacacc tgctccggaa
      121 tttcgtgtcc ggctaatttt aggtttaatg tataatcgga agcctgtgtg gtttgtagtt
      181 ttttgttttc taaaacaaat gtaaacggag ccggtttttc gaaaaaagta ttgtgtttaa
      241 ttaatcgttg cgaaccatcg cgcagcaaac tgggtgcggc aaacaatatt acaattagcg
      301 ttagcacagg taatgccgca tagcgcaggt attttttatt ggcacttaaa tcaatagcag
      361 ccgtaaaagg tacaggtctt agttcggcta ctttttgatc gatactggca tgtaataaag
      421 ctgacgattg ctgttgtaat tgttgttgca gttgtaaggt gtttagcagc ttatcgctaa
      481 cgttggtaaa atgttttcct atgatttgtg cagcttgctg atgactgatt atgctaccca
      541 gcttgtttaa ttttaagagt ggaatacaaa catatcgcac caaaaaataa acatttacaa
      601 gtatgaaact gtaaaataaa atggcgcgca tggttgtgtc gaaatgcccg atatattcaa
      661 gtgtggtaac ggtaatgcca aacactaaaa aagcggtaaa ggcataaata acgccacgca
      721 acagttggtt tttataatac ttacgtataa aggcatcgag tttttgaatt aactcattat
      781 agctgttcat gcttttaatt attaatgctg caatttaaaa attggttgcg gcttttaaag
      841 ttaacgtttt atgaatgacc attcttatga aatcatttat aaaaacgggt tgtattgtat
      901 tttggaactg tcaatgtgta aaaagaaaca gcccgttgtt acatgtaaca acgggctgcc
      961 tgtattaact agttattaaa actactgtgc cttaaaggtt acagtgtatg tttcaaccac
     1021 accgctcgag ttgtcggtaa aagtagcctt aaaatcagag ccactcagtt ctttaattgt
     1081 gtagctggta acggtaccac tttcagtaat tgaaagtacg gtttcgtctg tattaaatgc
     1141 ccaggtacca ttggtggttt gcgggtcgga tgggtcgcat ttggtagcac cttcgtcaaa
     1201 gttaacggtg ttgttgtctt taaaaataga taaatcatct tgcgagcagg caggtatttg
     1261 tgcatataca tcgcttatca aagtaccgcc aacattaaca ggtgggtcaa cggttaaggc
     1321 aaccattttc cagtttttgt cggtaagctt gtcggtattc gattcgtcat cgtctttttt
     1381 acaaccggta aaagttacgg ctaccatact taaaagcagg gcgcttgaaa ttaaattttt
     1441 catgttttta atgtttgagt tttagggcgc aaacataatt taaattttaa aaggtaagca
     1501 taattgctat aaggttgaaa gcaatcagat ctgtaaaaaa aaaattactc gcaaaagcgg
     1561 ccaaaaacgc ctaatggcaa ttgttgttgt gtactgcttt gtaaaaatat ttcaccggtg
     1621 tttacagcat ttttggtttt aaaagtatcg gccatcatgt tattaataat taatgacgat
     1681 aaacccaatg agtaggtatt aagcactaaa aacctttcgt tatcatcaag caattgtgca
     1741 actaatttaa gcagttggtt tatctggtct tctaatttcc agctttcgcc attggggccg
     1801 tggccaaatg ccggaggatc cagtataata ccattatatt ttttacctct ttttacttcg
     1861 cgcttggcat aggtaacggc atcttcaacc atccaacgga tgttgtttaa attactaagc
     1921 tccatgtttt cacgcgccca tgttactact tgcttaatgc tatctaaatg tgttacatcg
     1981 gcaccggcag cacatgcagc taaactggat gcaccggtat aggcaaataa atttaaaacc
     2041 ttaggtgcag gtgttttaaa ctgtttaacg ctatgataaa tgaaatccca gtttacggct
     2101 tgttccggga aaagacccac atgcttaaac gatgtaagcg ccaatttgaa ttgtaaggtg
     2161 gggccttgtg gtatgtcata actcatttgc cagcgttgcg gcatgttttt aacgttttgc
     2221 caaatgccac tactactcga ttttgaaaca aattttacat gcatttgttt ttgccactcg
     2281 gcattgctaa gtcttttgtc ccatatagct tgtggctcag gcctgcaggt aatgtaactt
     2341 ccccagcgtt ctaatttttc gaaattacca ctgtcaatta actggtattg actaaaattt
     2401 gtgggcgtta gtagttgcat ggtttaagcg ggttatagcg gcaaaggtta caacctgcgt
     2461 tttatacttt gacgcaagtt tttaatgcgg cctacgttaa ataagaatgc caaaaatagt
     2521 tttaccggaa atgacaacac ataccaaatt aataaaatat aattctgact aacgcctgag
     2581 ttggtgtagc cataagcgct gcgcaaattt ttagttacgc tttcgcagat tcgtatttca
     2641 gcatggttta gtcctttcaa aaacttattc ttattgcttt tattgatgcc taaagcttgg
     2701 ggattatcgc taccggttga cgaacccact tgcggcacac ttaacatatc agcactataa
     2761 gtaatatcta aaaaggcaca ggcttttttt aataacgttt cggggttttc aatcaggtct
     2821 tcaaatttta caaccattac ctgtggatga ctcacaagtt tttgaacttt gttgttgctg
     2881 ctattccaaa gtttgctgat ggtataggga tggtagttta agtatgcgcg tatggtttct
     2941 ttccagggaa tgtttttacc ggcacctaaa aatttgcgtt tccatttacc tttttgcgag
     3001 agcagcacat cgcgtgggtc gcgcaccatt ataattgctt ttgcagtagg gaaataagaa
     3061 aagatatcgg atgcaaatag caggtttagt ggtgtttggt cgcatcccat acttttatga
     3121 tgcagcttac tttcgtattg caaaaaataa gcaaacaaat tgggtgcaaa caaggcattg
     3181 ttggtagatt gagccagata gtttgcaata gctttttcgg cttcggctat atatttttca
     3241 gggtgctttt cgcgcaagta gccatcgcgc tggttactta ttaattttgt aaatacttca
     3301 accgcttgcg tttgcgtata atgttgttgc gggctgccgg gcgaccaaat gcgttcgaaa
     3361 aaatgaattt cttcgaatgt aaaaacagct tcatttttgc caaaaatacg acccatcatg
     3421 gtagtgccgc ttcggctggt gcccattaca aaaatcatgt tattgctcat aagtgtagcg
     3481 gtggaaaaat ctttttagta attaagctgt tgcattaaag ctaaaaaagg cttttctaaa
     3541 atggggttta aagcaatggg ttttttggca ggtaccggtt ttagtgtttg caatgcatag
     3601 gcattaagtg cggcatcggg ttgcaggtgg caaaaactgc gtatagtgtc taaggtattt
     3661 acaggttgcg ataccaaatc ttcgtatcga acaggcatta tatgttgcgg atatttggcc
     3721 attacctcta aacctttttt catggagatt atccactcaa caattgacat gtcaacctga
     3781 tttgtaaaag cttttatctc gttataatgc atttttaaat cttcgtcttg cggcaccaac
     3841 tcatcaacca acaaatgcca ttttcgatta ttaacgcccc accaatcgtg tgtttcgcct
     3901 tttacctgtt cgccttttac ctcgctccag ttggccgatg ataagcaggt atcccaacca
     3961 ttacggtata taaacaaggc tttaaaatcg ggaaaaatgc gttttaaaaa atcaatcctg
     4021 aaaatgagtt cggggtattt gtcaaccaca cgtttgctca taaccgtgta caggtaaaag
     4081 ccatagatgt tatggatgcg ttgtatgcgt tcgggtgtag cataagctgg gtctaggtaa
     4141 taagatgcat tactgcggct gtaactgcca atcaaatcct cgtccggaaa aactgaatgc
     4201 cacaatgctt taggttcgtt taaaaaagct atatcctgat gcatgcttaa aacaataccc
     4261 aaaatagttg taccgctgcg gcctgtgcct aaaataaaaa ccggctcttt aatttggttg
     4321 ctgttacgtt gttgcgcaaa atttaaaatt ttaaaaacca atggattaat ccattgcccg
     4381 ggcgtagtaa ccggcctgcc ttcgaacagt gcgtatgaca ccatacgcga gaaaaatttt
     4441 gtgggtcgtt tttgtatata ttctttatta atctgaccta tcatttcttt aaatcttatt
     4501 actaattcgc tttaaaagtt tgcttattac tttaaccaac ccggaaactg agtaaaccct
     4561 ttggttttta aataggctgc caattgtttg ttataaggtg catacatttc attgatgcga
     4621 ttttttattt cggcatcatg atcggtttta cttacgggtt gtccattaat cttataataa
     4681 atactgcgta agaatgattt aagttgttgg ttgttgcgcc aaaaacgttt agcaccaaag
     4741 ctcagtctta ccgctaacga atgcataaaa cggtttttat aattaaccga tttgtttttt
     4801 acttcgaaat tgtagttggc ataaaaaccg gggtcaacgc ctaaccaatc gcaaagtgat
     4861 aaaacaactt ttttcgaatc gcgcaggtcg tcaaaaaaca tcacttttaa attgtcgcca
     4921 aatacctgat accaggcatc aatatgttta atgtaatagc caaattcaaa accggtataa
     4981 aggtgatttt cttcgcgtag caagtcggct tcgctgtaat ttttacaatc atcaaaatat
     5041 tgctccagcg ttaaactttc gggcaattga aaaacttcgc gcttgcggtt aaaaaaagat
     5101 attacaattt gacaatagct tgctgcaatt tctgcgc
//
LOCUS       DAOMAA010000013         4953 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_10512_length_4953_cov_2.879134, whole genome shotgun sequence.
ACCESSION   DAOMAA010000013 DAOMAA010000000
VERSION     DAOMAA010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4953
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10512_length_4953_cov_2.879134"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            235..2955
                     /locus_tag="PLO59_00510"
     CDS             235..2955
                     /locus_tag="PLO59_00510"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU99599.1"
                     /translation="MKNFILLFVSVFTSVVIWQCKTTAPAPAGAIESRQRVRYELARL
                     CDPATGRIPPFMRMREITYANTLPSDALYKASSAVNFTLMGPNNLGGRTRALAIDVTN
                     ENIMLAAGVSGGVYRTTDGGNNWTKVGDVNGFQGVISIVQDTRPGHTQDWYFQTGEGY
                     GTSASAAGAFYLGNGLYKSTDGGLTWSGLSATNQNSPQGFNSNWEVGWNLAFDKSDTV
                     QTKLYAAVYGAIYKTTDGGNTWSYALGSSTNQSYFTDVAVTSTGVVYGTLSSDGASKG
                     IYRSVGGIGFVNILPAGFPATYDRIVIGIDPNNENVVYFFAATPNNGKYTYYFGDEWT
                     SLYKYEYISGNGQGAGGVWTNLSANLPDAGNTFNLMATQNGYDMAVRVMPGNSNVVFV
                     CGTNIYRSTTAFADSLNTTLIGGYQANSTMPIVFANQYEYPGHHPDQHNLAFLPSNPS
                     VMFSCSDGGISKTTDGLATNVQWNFINSNYTTSQFYALAIDHGAANNDIVIGGLQDNG
                     TWYTNSNNPNTPWIHSLGGDGAYCAISNTGHYYMSQQLGSTVKTDVDANGNVLAYTRI
                     DPVDGADYQWSNPFTLDPNNNNIMYLTAGRRLYRNSDLSALPTNNTWNKQNTNWTYFT
                     DTLTTAGELITAVAASTVPAHRVYYGTNKRKVFRIDNAHTGSPTATLLNIPANVLPAG
                     TGYVTCIAVNPQNADEIMVVLSNYNIYSLAFSKDGGSTWARCGGNLEQTATGSGNGPS
                     CRWATIAPVSNGTVYLVGTSVGLFATDTLIAPATVWTKQGVNTIGSNVVNMMDYRVAD
                     GMLAIATHGGGIFTTKITNNNVAAIAQLKENGISLMPNPVTDWLEVNITDASLNKTEQ
                     VSIVNQNGQIVMQLKPDEHLKINVSKLPIGLYYVNAIKGNKRWSKSFIKQ"
     gene            complement(3117..>4953)
                     /locus_tag="PLO59_00515"
     CDS             complement(3117..>4953)
                     /locus_tag="PLO59_00515"
                     /inference="COORDINATES: protein motif:HMM:NF020678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide-N-glycosidase F-related protein"
                     /protein_id="HQU99600.1"
                     /translation="PSVVSEVNSPLYDGNFNNFLLDGPNSNFNGTVNNSFQAISFPQI
                     APQLISTPSITLNATASSGLPVSYTVVSGPATVNGNVLSLTGAGTVVVAADQAGNAQY
                     NPAVQVVNTFDVLDPATTTPTIEARNPIDATNVYMSSLSAIDLAAIVTIVHPSLFNVQ
                     NVSFVINGNTIPATNHGNDHYTAIWTPPAYGPQTIQIVSTSNYNATSSVTLNINVVAP
                     VANLDSVQAFSGIIIDSSTPSKTVEGILPTYVDAYDSVMAVLTVTCPPGGCGPWDRVA
                     SIDARGKDGKWFEIIRYITPYGVACTHKINLMDYASLLQGKVTFRVNCATLDNGFSYA
                     LRFDFKEGTPQYKYSRVTQVWKDVYPFGDMANLQPVPAYNHTYWAGAVASKLKLVSTG
                     HGWGSLNTGNAAEFYNATHNIAVNGANTFVQNNWTICSPNPDACSPQNGTWTYNRAGW
                     CPGAIARPFDYDLTPYVAAGNLALGYQFQTSYTDQCHPNNPNCITGTTCADCADGFNP
                     ILDVNCNLITYFNNATYLSVDEINSTMLNVYPNPSKGIFNLNQTAKNYEAFDVQIFDL
                     VGNVIKSFAWEGNSMALDLSTYAKGIYILKAQNTKTLSVKRLVIQ"
BASE COUNT         1497 a          943 c         1063 g         1450 t
ORIGIN      
        1 agggcttgtt acagattttt aattcgaaag gtatttgtgt agcgcaacac gatgtgtttc
       61 aacaccagcc atttattaaa caagatttaa gtcaatggcc ggcaggcgtt tatcatgttg
      121 tattgattag tggtaaaaag caagtaacag aaagtttact agttaacgat aattagtaaa
      181 ggcaaaacca ttattttaat cttataaatt agtgtagtta taaacaaaat caaaatgaaa
      241 aattttattc tcctgttcgt atctgttttt acaagcgtag ttatctggca atgtaaaaca
      301 acagcgcctg cacctgcagg cgctattgaa agccgacaac gtgtaaggta tgagttagcc
      361 cgtttatgcg atccggcaac aggccggata ccacctttta tgcgcatgcg tgaaataact
      421 tatgcaaata ctttacccag cgatgcatta tataaagcaa gtagtgccgt aaactttaca
      481 ttaatggggc ctaataattt aggtggccgc acacgtgcac tggctattga tgttaccaat
      541 gaaaacatta tgcttgctgc cggtgtttcg ggtggtgtgt atcgcacaac cgatggcgga
      601 aataactgga ctaaggtggg cgatgttaat ggctttcagg gtgtaatttc aatagtacag
      661 gatacaaggc cgggccatac gcaggattgg tatttccaaa ccggtgaagg ttatggaact
      721 tcggcaagtg cagcaggcgc tttttatttg ggcaatggtt tgtataaatc aacagatggc
      781 ggcttaacat ggagtgggtt aagtgccacc aaccaaaact cgcctcaggg ttttaacagc
      841 aattgggaag taggctggaa cctggctttc gacaagtcgg ataccgttca aaccaaatta
      901 tatgcagccg tttatggtgc catttacaaa acaactgatg gcggcaatac ttggagttat
      961 gctttaggca gtagtaccaa tcaaagttat tttacagacg ttgctgttac ttcaacaggt
     1021 gtagtttatg gcacacttag tagcgatggt gcatctaaag gtatttaccg atcggttgga
     1081 ggtattggct ttgtaaacat tttaccggct ggtttccccg ccacttatga tagaattgta
     1141 attggtattg atccaaataa tgaaaatgta gtttactttt ttgctgctac gccaaacaat
     1201 ggtaagtaca cttattattt tggcgatgaa tggacaagct tatacaagta tgaatacata
     1261 agcggtaatg gccagggtgc aggtggcgtg tggacaaatt tgtcggccaa tttgcccgat
     1321 gccggtaata cctttaattt aatggctaca caaaacggat atgatatggc tgttcgcgta
     1381 atgcccggta acagtaatgt agtttttgta tgcggtacca atatttacag aagtacaaca
     1441 gcttttgccg attcgcttaa caccacctta attggcggat accaagccaa cagcaccatg
     1501 cccattgttt ttgcaaacca atacgaatat ccggggcatc atcccgatca gcataacctg
     1561 gcttttctgc catctaatcc atcggtaatg tttagttgca gcgatggtgg tatttctaaa
     1621 actactgatg gtttagctac taatgtgcaa tggaatttta ttaattccaa ttacaccact
     1681 tctcaatttt atgccttggc tattgatcat ggcgcggcta acaacgatat agttattggg
     1741 ggcttacaag ataatggcac ctggtacacc aacagcaata atccaaacac accttggata
     1801 cacagcttgg gaggcgatgg agcttattgt gccataagca atacgggcca ttattatatg
     1861 agccaacaat tgggatccac tgttaaaacc gatgtggacg ctaatggaaa tgttttggca
     1921 tacacacgta tcgatccggt tgatggggcc gattaccagt ggagtaatcc atttacttta
     1981 gatccgaaca ataacaacat tatgtatttg acagcaggcc ggaggttgta tcgcaacagc
     2041 gatttatcgg ctttgccaac taacaacacc tggaacaaac aaaatacaaa ctggacctat
     2101 tttaccgata cactaactac tgcaggcgag ttaataactg ctgtagctgc atcaaccgta
     2161 cctgcacatc gtgtttacta tggtaccaac aagcgtaaag tttttaggat tgataatgca
     2221 catacaggaa gccctactgc cacattatta aatataccgg ccaatgtatt acctgcaggt
     2281 acgggttacg ttacctgtat tgccgttaat ccgcaaaacg ctgacgaaat tatggttgtg
     2341 ttgagcaact ataacattta tagtttggca tttagtaaag atggaggaag cacctgggcg
     2401 cgttgtggcg gtaatttaga acaaactgca acaggcagtg gcaatggccc aagttgccgc
     2461 tgggctacca ttgcaccagt tagcaatggc actgtttatt tagtgggtac cagtgtgggc
     2521 ttgtttgcta ccgacaccct gatagcaccg gctacggttt ggacaaaaca aggtgtaaat
     2581 actattggca gcaatgtggt taatatgatg gactataggg ttgccgatgg aatgctggca
     2641 attgctactc atggtggcgg aatatttaca accaaaataa ccaacaataa tgtggcagcc
     2701 attgcacaac ttaaagaaaa tggtatttcg ctaatgccta accccgttac cgattggtta
     2761 gaagtaaata taactgatgc atccttaaat aaaacagagc aggttagtat cgtaaaccaa
     2821 aacggacaaa ttgtgatgca attaaaaccg gacgaacact taaaaattaa tgtaagtaaa
     2881 ttgcctattg ggttgtacta tgttaatgca attaaaggca ataaacgttg gtcgaaaagt
     2941 tttatcaaac aatagcaaga ttgtttttaa gtattgaaaa taatgtggca atgcttctaa
     3001 taaaaggttt gaaatagcgc cagtttagtt acaaacaaac aagccccgta attattacgg
     3061 ggcttgttta ctaaataaat attcaagtat tacttaatgt aatacgaaag cagaacttat
     3121 tgaattacca atcgttttac cgatagtgtt tttgtgtttt gtgcttttaa aatataaata
     3181 ccttttgcat aggtacttaa atctaaagcc atgctgttac cttcccaggc aaacgattta
     3241 atcacattac ctactaaatc gaaaatctgc acatcaaaag cttcatagtt tttagctgtt
     3301 tgatttaaat taaatatgcc ttttgatggg tttggataaa cattaagcat ggtgctgttt
     3361 atttcatcaa cacttaaata ggttgcatta ttaaaataag taatcaggtt gcagttaaca
     3421 tctaaaatag gattaaatcc atctgcacaa tcggcacagg tagtaccagt aatacagttg
     3481 gggttattgg gatggcattg gtcagtatag cttgtttgaa actgatagcc taatgccaaa
     3541 ttaccggcag ctacataagg cgttaaatca taatcaaacg ggcgtgctat tgcgcccggg
     3601 caccagcctg cacggttgta agtccaggta ccattctgtg gcgagcatgc atcggggttt
     3661 ggcgaacaaa ttgtccagtt gttttgcaca aaggtattgg ctccatttac cgctatgtta
     3721 tgtgtagcat tataaaattc ggcagcattg ccggtgttta aactacccca accatgacct
     3781 gtacttacca attttaattt gctggctacg gcacctgccc aataagtatg attgtatgca
     3841 ggtacaggtt gtaaattggc catatcacca aaaggataaa catctttcca aacttgcgtt
     3901 acacggctgt atttgtattg tggtgtacct tctttaaaat cgaaacgcaa tgcataagaa
     3961 aagccattat ctaaagtagc gcagttaacc ctaaaagtta ctttaccttg cagcaatgat
     4021 gcataatcca tcaggttaat cttatgcgtg catgcaacgc cataaggtgt tatgtaacgt
     4081 ataatttcaa accatttgcc atccttgcca cgtgcatcaa tactggcaac tctatcccag
     4141 gggccacagc cacccggagg gcaggtaacc gtaagtacag ccattacgct atcataagca
     4201 tctacataag ttggtaatat gccttcaacg gttttagaag gtgtgctgct gtcaattata
     4261 ataccgctaa atgcttgcac gctatctaag tttgcaacag gcgctactac atttatatta
     4321 agtgtaacac tactggtggc attgtaatta ctggtactta caatttgtat ggtttgtggg
     4381 ccataagccg gtggtgtcca tatagctgta taatgatcgt taccatgatt ggtagccggt
     4441 atggtattgc catttataac aaaagaaaca ttttgaacat taaataaact tggatgtaca
     4501 atggtaacaa tggctgccaa atcaattgcc gatagtgacg acatgtaaac attggtagca
     4561 tcaatcggat tacgtgcttc gatggttggt gttgttgttg cagggtctaa tacatcaaaa
     4621 gtatttacca cttgtactgc agggttatat tgcgcattac ctgcctggtc ggcagctaca
     4681 acaacggttc ctgcaccggt taatgaaagt acattaccat ttacagtagc cgggcctgat
     4741 acaacggtat agcttaccgg taagcccgat gatgcagttg catttaaagt aattgatggc
     4801 gtagaaatta gttgtggtgc aatttgcgga aatgatattg cttgaaaact gttgttaacg
     4861 gtaccgttaa agttagagtt tgggccatcc aataaaaagt tattgaagtt accatcgtac
     4921 aacggtgaat ttacttcgct aacaacgcta ggt
//
LOCUS       DAOMAA010000014         4879 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_10809_length_4879_cov_2.972637, whole genome shotgun sequence.
ACCESSION   DAOMAA010000014 DAOMAA010000000
VERSION     DAOMAA010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4879
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10809_length_4879_cov_2.972637"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..1508)
                     /locus_tag="PLO59_00520"
     CDS             complement(105..1508)
                     /locus_tag="PLO59_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99601.1"
                     /translation="MKNNFLKQFLNGGQQQYLFFFCIVLLSRIPFINAGYGAEEDSWG
                     MMNAMQRMALTGRFEISRFPGHPIPEFIFGLMPFTPYYVINICTAFLSALAAVFFAAV
                     LNQYKIKKAFWYAAALSFIPVIFIKSTDNMDYVWALAFIIMAWYFFLRQWLVLSAVLL
                     ALAFASRFTSIVFLPSVLMLLYFEHKKWKSVTTYALVFIVTVIICYSQIIIHYDSKVY
                     VVPYILGFPDLLKTLYKSSIGVWGLLGCVAACGMIIQIAVGNCIINRQNKMVVWLALV
                     LALLLFIWQPHKAAYLIVALPFLVFLWAVYGNGKYVTSITCLLIASNFLGGLYLKDAY
                     RTKQPGSIELKIAGQPILFDVCKGPLLAEHEKRLQKIDFINTVLDTLPLLKQPAIILS
                     GWYTNELEVLARRRHITLNCMYFMEPQLLEKNCAQHITVYFLPQQDAVNDIRWKVSNL
                     TKNFSQPLFKSNTPFIF"
     gene            complement(1498..3093)
                     /locus_tag="PLO59_00525"
     CDS             complement(1498..3093)
                     /locus_tag="PLO59_00525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HQU99602.1"
                     /translation="MKYILQPLIYAVLIVAGIFIGLFLLPSPQGYTSIKRSELNSGKI
                     ADIIQFIQNEYVDTVNTTQLVDNTIEQMMQQLDPHSAYIPASELKQVNEPLRGNFEGI
                     GIEFHISRDTVMVVNVIAGGPSAAVGLMAGDRIVKVDGKNFYGAQITNDTVLKTLRGQ
                     GGTKVKLSIHRSGTGKLIDYTITRGNIPIYSVDAAYMADSETGYIRMSRFGEKTYDEY
                     MEAYEKLNGQGMQKLILDLRGNGGGFLEIAQQIADEFLPDGKMIVYTAGRKQPRKNYE
                     ATANGKFETGKLVVLIDEGSASASEIVAGAVQDWDRGTIVGRRSFGKGLVQQQTELQD
                     GSALRLTIARYYTPTGRSIQKAYNQGYDDYENDLNNRYDHGELLNADSIKFADSLKYK
                     TPSGKIVYGGGGIMPDVFVPLDTAGRNRFTDILYASGALNEFCYKYVDENRNVLKSKF
                     SETAFLNSFQIDATIAQKLKSYLRKQDITTDDATFNKALTQTSNQMKAIMGRQLYNNE
                     VMYKVLQQDDKALKKALQILNEK"
     gene            complement(3184..3648)
                     /locus_tag="PLO59_00530"
     CDS             complement(3184..3648)
                     /locus_tag="PLO59_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013930963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HQU99603.1"
                     /translation="MRSFDDIYMDLAQNIALKSHCVKTKVGAVLAKDTRIISLGYNGP
                     PAHTHNCDEEWPGTGCQRSIKGGCSLALHAEENAILYAAKNKVDLDGATLYVTLSPCL
                     SCARTVYTMGIKKVVYLNSYAAYKGHGVEEGLDFLLRFGVEVCQYKPQSITA"
     gene            complement(3697..>4879)
                     /locus_tag="PLO59_00535"
     CDS             complement(3697..>4879)
                     /locus_tag="PLO59_00535"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HQU99604.1"
                     /translation="YHKKWKGEMTGLRKNNVVQLKRKQESNFQYWTTMNTEGKPYTNI
                     LSDFKMAYAQYKPISNAVDYTNEAVMGIELMNYAKLYTKLVNLSSTDSVADSVLTAEA
                     HKVLKNSEPWFKKYRTQIDEEIFAAMLKMYGDSVAQAYRPEYFDKTLYGKYAGNYNKW
                     AADVFKKSFMTNYTSVEKMLAAYKRSNAKKISNDMAYQLMYAITTQYDQVISKKAVSI
                     NQQIAQIQRSYMQALRLFNKEKTLYPDCNSTLRVTYGQVKGYTPRNGIYYNYQTWLKG
                     TIEKNETGNADYVMPVKLRQLIEQKDFGPYAVNGDVPVSFIATNHTTGGNSGSPVLNA
                     YGQLIGTNYDRVWEGTMSDVYYDPSICRNITLDIRYTLFIIDKYAGAANIINELDIVK
                     Q"
BASE COUNT         1420 a          994 c          876 g         1589 t
ORIGIN      
        1 cacaccacaa taaaatgctg caggcagttt ataccacaag taaaatgcaa actgccattt
       61 actatttaat ttgctcagaa gttttgttgt tgccatgccc atgtttaaaa aataaaaggt
      121 gtgttacttt taaataaagg ttgcgaaaag tttttagtaa ggttgcttac cttccagcgt
      181 atgtcattta ctgcatcctg ttgtggcaaa aaataaaccg ttatatgttg tgcacaattt
      241 ttttctaaca gttgcggctc cataaaatac atgcaattta atgttatgtg ccgcctacgt
      301 gccagcactt ctaactcgtt ggtgtaccag cctgataaga ttatggcggg ttgttttaac
      361 aggggcagcg tatctaaaac ggtatttata aaatctattt tttgcaatcg tttctcatgc
      421 tcggcaagca acgggccttt acatacatcg aataaaatag gttggccggc tatttttagt
      481 tcaatgcttc cgggttgttt tgtgcggtat gcatccttta agtacaagcc gcccaaaaaa
      541 tttgaagcaa ttaataaaca ggtgattgat gtaacatact taccattacc ataaacagcc
      601 cataaaaaaa ctaaaaaagg taatgctaca attaagtatg ctgctttatg tggttgccat
      661 ataaataaca gcaaagccaa aaccaaagcc aaccatacaa ccattttgtt ttgcctgttt
      721 ataatgcaat tgccaactgc tatttgtata atcataccac atgcagctac acatcccaat
      781 aaaccccaaa caccaattga agacttgtac aaagttttta gcaaatcggg gaaacctaaa
      841 atataaggca ctacataaac tttactatca taatgtatta tgatttgact atagcatatt
      901 ataacagtta ctataaatac aagtgcgtaa gtagtaaccg acttccattt tttgtgctca
      961 aaatataaaa gcattaaaac cgagggtaaa aacactatag acgtaaagcg cgatgcaaaa
     1021 gccagtgcca ataatacagc agacaacacc aaccattgcc gcagaaaaaa ataccaggcc
     1081 ataattataa acgccaaggc ccatacatag tccatattat cggttgactt aataaaaata
     1141 accggtataa atgacaaagc agcggcatac caaaaagctt ttttaatttt atactgattc
     1201 agtactgctg caaaaaaaac tgctgccaat gcacttaaaa aagcggtgca aatatttatt
     1261 acataatagg gtgtaaacgg catcaggcca aaaataaatt cgggtatggg atgcccaggg
     1321 aaacgcgata tttcaaacct gcctgtaagt gccatacgtt gcatggcatt catcattccc
     1381 cagctatctt cctcggcacc gtagcctgca tttataaatg gtattcgcga tagaaggaca
     1441 atacaaaaaa agaacagata ttgctgttgg ccaccattta aaaactgctt taaaaaatta
     1501 tttttcattt agtatttgca gtgctttttt taaagcctta tcatcttgtt gcaatacttt
     1561 atacattact tcattgttat ataactgcct gcccataatt gccttcattt gattacttgt
     1621 ttgggttaag gctttattaa aggttgcgtc atcggttgta atatcttgtt ttcgcaaata
     1681 gcttttcagc ttttgagcaa tagtggcatc aatttgaaat gaatttaaaa aagcagtttc
     1741 agaaaatttg ctttttaaaa cattccggtt ttcgtcaaca tacttataac aaaactcatt
     1801 taaggcaccg cttgcataaa gtatatctgt aaagcgattg cggcctgcag tatctaatgg
     1861 cacaaataca tcgggcatta tgcctccacc gccataaaca attttgcctg atggtgtttt
     1921 gtatttaagc gaatcggcaa atttaatgct gtcggcattt aataactcgc catgatcgta
     1981 ccggttgttt aaatcatttt cataatcatc ataaccctga ttgtatgctt tttgaatact
     2041 gcgtccggtg ggtgtataat aacgcgctat ggttaaacga agtgcactgc catcttgtaa
     2101 ctcggtttgt tgttgcacca atcctttgcc aaacgacctg cgccctacaa tggtgcctct
     2161 gtcccaatcc tgaacggcac cggccacaat ttcgctggcc gatgcactgc cttcgtcaat
     2221 aagtactacc agcttgcccg tttcgaattt accattagct gttgcttcgt aatttttacg
     2281 tggctgctta cgacctgctg tgtaaacaat cattttacca tcgggcaaaa attcatcggc
     2341 tatttgctga gctatttcta aaaagccccc accattgccg cgtaaatcta aaatgagttt
     2401 ttgcatgccc tggccattta atttttcgta tgcttccata tattcatcat acgttttttc
     2461 gccaaaacga ctcatgcgta tatagcccgt ttcgctatcg gccatgtaag cggcatcaac
     2521 gctgtaaatg gggatgttgc cccgggtaat tgtatagtca attagtttac cagtaccact
     2581 acgatgtatt gatagtttaa cctttgtacc tccctgacca cgtagtgttt ttaaaactgt
     2641 atcgtttgta atttgtgcgc cataaaaatt tttgccatca acttttacaa ttctatcacc
     2701 ggccattaat ccaacagcag ccgaagggcc tccggcaatt acgtttacta ccatcacggt
     2761 atcgcggcta atatgaaact caatacctat gccttcgaaa ttaccacgca aaggttcgtt
     2821 aacttgtttg agttcgcttg caggtatgta agccgagtgt ggatctaatt gctgcatcat
     2881 ttgttcgatg gtattatcaa ccaactgcgt agtgtttaca gtatctacat attcgttctg
     2941 tataaactgt atgatgtcgg caattttacc tgagtttaat tcgcttcgtt ttatggatgt
     3001 atatccttgc gggcttggta ataaaaacaa accaataaaa atgcctgcca caattagcac
     3061 tgcataaatt aaaggttgca aaatgtattt cataaattac tatttattcg tttttgcagc
     3121 tggcctgttt tggtgctgct ttaattgacg cgtttgcata gttttaagtt tggcattaaa
     3181 actttatgct gtaatacttt gtggtttgta ttggcatacc tctacaccga agcgtaataa
     3241 aaaatcaagt ccttcttcaa ccccatgtcc tttgtaggct gcataagagt ttaaatacac
     3301 tactttttta atacccatag tgtaaaccgt acgggcacaa cttaagcaag gcgaaagggt
     3361 aacgtatagc gttgctccgt ctaaatcaac tttgttttta gcggcataaa gtattgcatt
     3421 ttcttcggca tgcagtgcca atgaacaacc gccttttatt gagcgttggc aaccggtacc
     3481 gggccactct tcgtcacaat tatgggtatg ggcaggcggg ccattgtagc ctaatgaaat
     3541 aatgcgcgta tccttagcta aaactgcgcc taccttggtt ttaacgcaat gcgatttaag
     3601 cgcaatgttt tgcgccaaat ccatgtaaat atcatcaaag cttctcatac aaatattcaa
     3661 ttgtaattat ttaataacct actgtataaa cgtagtttat tgctttacaa tatctaattc
     3721 atttataatg ttggcagcac ctgcatactt atcaattata aacaatgtat agcgaatgtc
     3781 taacgttatg tttcggcaaa tgcttgggtc ataatacaca tcgctcatag taccttccca
     3841 aacgcggtca tagttggtgc caataagctg gccataagca ttaagcaccg ggctgccact
     3901 gttaccaccc gtagtatggt tggttgcaat aaaacttacg ggcacatcgc cattaacggc
     3961 ataaggacca aaatcttttt gttctatcaa ttgacgcagt tttacgggca ttacataatc
     4021 agcattaccg gtttcatttt tttcgatggt gccttttaac caggtttgat agttgtaata
     4081 aataccattg cgtggtgtgt agcctttaac ctggccatag gttacacgca gtgtgctgtt
     4141 acaatcgggg tacagtgttt tttctttatt aaacaaacgc aaggcttgca tatagctgcg
     4201 ttgtatttgc gcaatttgct gatttatgga taccgctttt ttcgaaataa cctggtcgta
     4261 ctgggtggtt attgcataca ttaactgata agccatatcg ttacttattt ttttggcatt
     4321 acttcgttta tatgcagcaa gcattttttc taccgaggta tagttagtca taaacgattt
     4381 tttaaataca tcggctgccc atttattata gttgcctgca tattttccat ataacgtttt
     4441 atcaaagtac tcagggcggt atgcctgcgc tacactatcg ccatacattt taagcatggc
     4501 tgcaaaaatt tcttcatcaa tttgggtacg atatttttta aaccacggct ctgagttttt
     4561 taaaacttta tgggcttcgg ctgttagtac actatcggca acgctatcgg tactgctaag
     4621 gtttaccagt ttggtataaa gttttgcata gttcatcagt tcaataccca taacggcttc
     4681 gttggtataa tctacagcat tggatatagg tttgtattgt gcataagcca ttttaaaatc
     4741 agacaatata tttgtgtatg gtttgccttc ggtattcata gttgtccaat actgaaaatt
     4801 actttcctgt ttacgtttta gttgcaccac attgttttta cgcagtccgg tcatttcgcc
     4861 cttccacttt ttatgatag
//
LOCUS       DAOMAA010000015         4871 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_10845_length_4871_cov_2.945598, whole genome shotgun sequence.
ACCESSION   DAOMAA010000015 DAOMAA010000000
VERSION     DAOMAA010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4871
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10845_length_4871_cov_2.945598"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            334..1344
                     /locus_tag="PLO59_00540"
     CDS             334..1344
                     /locus_tag="PLO59_00540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007093580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQU99605.1"
                     /translation="MKTAIVILNYNTRTLLQQFLPVVIQHSTSAHVIVADNGSNDDTV
                     AFMKNEFAQVALIVNKVNGGFAAGYNQCLRHVNAEYVLLLNSDVLVTENWIEPLQNYL
                     DINPEVAAVQPKILDYKNQTKFEFAGACGGFIDCLGFPFCRGRIFDSIEADNGQYNQP
                     IEVFWATGACMLIRKKIFDDLGGFDEMFFAHMEEIDLCWRIQNAGYKLMVVPQSVVYH
                     VGGGTLAVTSPRKTYLNFRNNLLMLHKNLPGHLLFSTLFAKMTLDGIASVKFLFSNGW
                     QHFLAVFKAHMFFYSNIFSIQKQRSITQSMVVYHNRKNIYKHSIVWKYYIKRIRCFSK
                     LH"
     gene            1490..2824
                     /locus_tag="PLO59_00545"
     CDS             1490..2824
                     /locus_tag="PLO59_00545"
                     /inference="COORDINATES: protein motif:HMM:NF028437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5106 domain-containing protein"
                     /protein_id="HQU99606.1"
                     /translation="MYSIKVQVPGIPDSMCFLANHFGDKQYLQDSCKADAKGNFEFKG
                     EKTLSRGMYLIVLPNKRYFEIVLDQTFQFSIKADSSLNPSKISFSGSVENTEFYNWLT
                     YINDKRADMEKTRNAYNLLPESQRSNSAEFKHLKQIEQEIETYQQKYIDEKPKSFLSA
                     IYLATREIDIPDAPILANGLKDSTFQFYYYRNHYFDNINFGDARLLRTPIFHQKLDYY
                     MHKLVLQQPDSIIAEADKLVALAEKDTEVFKYTVWYITHEFETSKIMGMDAVFCHMVK
                     KYYTRDKAYWLDDASLFKIQDRAKQLDPILLGKKARNIVLTDSNNFAQSLYAIKEPWT
                     VLFFWDPDCGHCKKSMPKLIEFYNEWHKKGVEIFAVCNEIEMDKWKAFIKENNLQWIN
                     VADPNLRDNFRYDFDVATTPQVFVLDKDKIIRAKKLDVTQLGDFLKHETDLK"
     gene            complement(2906..4504)
                     /locus_tag="PLO59_00550"
     CDS             complement(2906..4504)
                     /locus_tag="PLO59_00550"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU99607.1"
                     /translation="MKLRHVIALVFVLTISKTYAQQSWTTPPLQWEQNYGGTANDRAN
                     DVIPTSDGGFIAVGNSMSDDVDLTNNCGLNDVWVVKTDGNGQVQWQRNYGGDDEDFGL
                     SIAQTFDGGYIIGATTFSNNNNVSGNNGFSDFWVLKIDSVGVIEWSKVYGGSLLESLF
                     QVKQLRDSSYVAVGSTDSNDGDVSGNHGESDYWVVRLNLAGDTIWTQCYGGLKTDVCR
                     DISQLVNGNFVLAGYSNSNDGDLTSNNGGYDYWVLRIDHNFKNIIWQYNYGGSYDDRA
                     YSLTLNSALGYLVIGSSYSNDINVSGHSGSVGPTGNADVWALKLTNLGIIDWENSLGG
                     LADDFAFDAVQLADSSYTIAGYTSSLLANTSPNNGLTDYWLINVNRFGNRQWGMVYGG
                     SKADFCFAIDTLSSFTYIIGGGSFSNDSVVGGNYGGSDYWLLRLDSAKNTTALPDVSA
                     DGNQLTIYPNPLRTQSTISIKTNHTDKQLLNIYNNIGQIIKSIDVTGVTSLDIHRNNF
                     QAGVYHYTLGYENKILTRGKFVVE"
BASE COUNT         1616 a          872 c          846 g         1537 t
ORIGIN      
        1 taaataatgc aagccttaaa aaaacgaaca ataaaaaaat aaactgaatt gtaaatccag
       61 ctacaaatac tccaaaaagt acttggcaaa aaattaaaaa atcaaaagta tatggtaatg
      121 aaaacagctc catcaatttt tagggaaagg cgggcgaaag tatattattt tgtacctttt
      181 ttctgatgca gttctatgca agatattaac atggcagttt tggggtagtt gcttcatgca
      241 acgtttaatt taagtagtaa aattacggtg tttatttagc tttgccggcc tttgtaaatt
      301 ttttcatcat cagatattta agcggcaccc gcattgaaaa cagccatagt aatattaaac
      361 tacaacacac gtacattgct gcaacagttt ttgcctgttg ttatacaaca cagtacttcg
      421 gctcatgtga tagttgccga taatggcagc aatgacgata cggtagcttt tatgaaaaat
      481 gaatttgcac aagtagcgct tattgttaat aaagtaaacg gtggttttgc tgccggttat
      541 aaccaatgcc tcaggcatgt aaatgccgaa tatgttttat tattgaatag cgatgtattg
      601 gttactgaaa attggattga gccattacaa aattatttag acattaaccc cgaggttgct
      661 gctgtacagc ccaaaatttt ggattataaa aaccaaacta agtttgaatt tgcaggtgca
      721 tgtggcggat ttattgattg cctgggattt cctttttgca gaggccggat tttcgattcg
      781 attgaagcag acaatggcca gtataatcaa cccatagaag tgttttgggc aacgggtgct
      841 tgtatgctga tacgtaaaaa aatatttgat gatttaggcg gtttcgatga aatgtttttt
      901 gcacacatgg aagaaattga tttgtgttgg cgcatacaaa acgcaggtta taaactgatg
      961 gttgttccac aatcagtagt atatcatgtt ggtggtggca cgttggctgt aactagccca
     1021 cgcaaaacct acctcaattt cagaaacaat ttattaatgc tgcataaaaa cctgcccggt
     1081 catctgttgt ttagtacctt atttgctaaa atgactttag atggtatcgc atcggttaag
     1141 tttttgttta gcaatggttg gcaacatttt ttggctgtat ttaaagcaca catgtttttt
     1201 tattcaaata ttttttcaat tcaaaagcag cgtagcataa cgcaaagcat ggtagtttac
     1261 cacaatagaa aaaatatata caaacacagt atcgtttgga aatactatat aaaacgcata
     1321 aggtgtttta gcaagctaca ttaattgttt tgatttctta ataactactt aaatcatcaa
     1381 aaatttaaat gcttcaattt aaattgcacg catcaacgca tttaatcata tgaaaaaatt
     1441 tattgtattt tttagcatcc tttttgctgc aaatgttcaa gccaaaaaca tgtattcaat
     1501 taaagtgcag gtgccgggta ttcccgactc tatgtgcttt ttagctaatc attttggaga
     1561 taagcaatac ctgcaagaca gttgcaaggc cgatgcaaag ggcaattttg aatttaaagg
     1621 cgaaaaaaca ttaagccgtg gcatgtactt aattgtatta cccaataagc gttatttcga
     1681 aattgtttta gaccaaacat ttcagtttag catcaaagcc gattcatcgc tcaatccatc
     1741 aaaaatttca tttagcggat ctgttgaaaa tactgagttt tataattggc taacctatat
     1801 aaatgataag cgagccgata tggaaaaaac gcgtaatgca tacaacctgt tacccgaatc
     1861 gcaaagaagc aactcagcag aattcaagca cctaaaacaa attgagcaag aaattgaaac
     1921 ctatcagcaa aaatatatag atgaaaaacc aaaatcattt ttaagcgcta tttatctggc
     1981 aacacgcgaa attgatattc cggatgcacc aatacttgcc aatggtttaa aggattcaac
     2041 atttcaattt tactattatc gtaatcacta tttcgataat ataaactttg gagatgccag
     2101 acttttacgt acgccaattt ttcatcaaaa attagactat tatatgcata aactggtttt
     2161 acagcaaccg gatagcatta tagccgaagc cgataagttg gttgcccttg ccgaaaaaga
     2221 tacagaggta tttaaataca cagtttggta tattactcat gagttcgaaa catcaaaaat
     2281 tatgggcatg gatgctgtgt tttgtcatat ggttaaaaag tattatacgc gcgataaggc
     2341 ttattggtta gacgatgctt cgctatttaa aatacaggac agggccaaac aactcgaccc
     2401 tattttgtta ggtaaaaagg ctagaaatat tgtgcttacc gattcgaaca attttgcaca
     2461 atcgctttat gctataaaag aaccgtggac tgttttattt ttttgggacc ctgattgtgg
     2521 tcattgcaaa aaatcaatgc ctaagcttat tgagttttat aatgaatggc ataaaaaggg
     2581 ggttgaaata tttgccgtat gtaatgaaat tgaaatggat aaatggaaag catttattaa
     2641 agaaaataac ttgcaatgga ttaatgttgc cgatccgaat ttacgcgata atttcagata
     2701 tgattttgat gttgctacca cacctcaggt ttttgtttta gataaggata aaattataag
     2761 agccaaaaaa ttagatgtta cacaacttgg cgacttttta aagcatgaaa cggatttgaa
     2821 atagttttat aacttgtaaa taataaaaca aaaaagcctg ctcatttatt tggagcaggc
     2881 tttttttaca acgttttggt tttacttatt caacaacaaa tttaccgcgc gttaaaattt
     2941 tattttcata accaagtgtg tagtgataaa ctccggcttg aaaattattt cgatggatat
     3001 caaggcttgt tacaccggta acatcaattg atttaattat ctgaccaata ttattataaa
     3061 tattcaacaa ctgtttatcg gtgtggttgg ttttaattga tatggtagat tgtgtacgaa
     3121 gcggattagg atatatggtt aattgatttc catctgccga aacatcaggc aaagcagttg
     3181 tgttttttgc tgagtctaat ctaagcagcc aataatcgct gccgccataa ttgccaccta
     3241 caaccgagtc gtttgagaaa gaaccacctc ctataatgta tgtaaaactt gatagggtat
     3301 caatagcaaa acaaaaatca gccttcgagc caccataaac cataccccat tgcctgtttc
     3361 caaaacggtt tacattaatc aaccaataat cagttaaacc gttattgggg ctggtatttg
     3421 ctaataagct ggatgtgtag cctgcaatag tatagctgct atcggccaat tgtactgcat
     3481 caaaagcaaa atcgtcagct aagccgccta atgaattttc ccagtcaatt atgcccaggt
     3541 tggtaagttt taatgcccaa acatcggcat tgcccgttgg gcccacacta ccactatgcc
     3601 ctgatacatt tatatcgtta ctatacgaac taccaataac taaatatccc aatgcggagt
     3661 taagggttaa cgaataggct ctgtcatcat aactgccacc atagttgtat tgccaaataa
     3721 tgtttttaaa attatgatca atgcgtagca cccaataatc ataaccacca ttgttgctgg
     3781 ttaaatcacc atcgttcgaa ttcgaatatc cggctaacac aaaattgccg ttaaccaatt
     3841 ggcttatatc gcggcaaaca tctgttttta gtccaccata acattgtgtc caaatggtat
     3901 caccggctaa gtttaaccgc accacccaat aatccgattc gccatggttg cccgatacat
     3961 caccatcgtt cgaatcggta ctgcctacgg ccacataact gctatcgcgt aattgtttta
     4021 cctgaaataa actttctaat aaactgcctc cataaacttt cgaccattca ataacgccta
     4081 cgctatcaat ttttaaaacc caaaagtctg aaaacccatt attgccactt acattattat
     4141 tgttcgaaaa tgtagtggct ccaataatat agccaccatc aaaagtttga gcaattgata
     4201 aaccaaaatc ttcgtcatcg ccaccgtaat tgcgttgcca ctgtacctgt ccattaccat
     4261 cggtttttac tacccaaaca tcgtttaatc cacagttatt ggtaagatca acatcatcac
     4321 tcatcgaatt gcctacagct ataaatccac catctgatgt gggtataaca tcatttgctc
     4381 tgtcgtttgc cgtacctcca taattttgct cccattgtaa gggtggtgtg gtccaacttt
     4441 gttgtgcata ggttttgcta attgttaata caaaaactaa tgcaattaca tgtctcaatt
     4501 tcatcgtcaa tttttttatt tgttaataaa atgcgggggt taaaatggat tgcaaattta
     4561 atataataaa ttgccacaca aaagctatac caacaactaa agtttaaact agggttaaac
     4621 aagatttata taggttgtaa taaatttaca tcgctgttgc aatttaacaa catacattgc
     4681 tataaaccgc taaaaatttg aatagagctt aaacaaaaat gattttttga ttgtttgctt
     4741 taagccaatt ataatgcaaa cgttggtatt acataagcaa cataaagtaa gcgttgcaca
     4801 aacgttaatt tataagatga aagtaaaaag ggagtagtac cagtcactta taaagcaggt
     4861 ttgttttttg t
//
LOCUS       DAOMAA010000016         4860 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_10887_length_4860_cov_3.247242, whole genome shotgun sequence.
ACCESSION   DAOMAA010000016 DAOMAA010000000
VERSION     DAOMAA010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10887_length_4860_cov_3.247242"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="PLO59_00555"
     CDS             <1..394
                     /locus_tag="PLO59_00555"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HQU99608.1"
                     /translation="TLPKRKAICKFYNEALSKYNWAVLPTQQLADIESSYHLYPLRIK
                     NINEAQRDLIIQKIFDADVSVNVHFIPLPMLSYYKNLGYDINHYPNTYQNYASEISLP
                     VFFDITEAQLTSVINAVVNAVTEILKLD"
     gene            409..984
                     /locus_tag="PLO59_00560"
     CDS             409..984
                     /locus_tag="PLO59_00560"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003408041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HQU99609.1"
                     /translation="MDITVAMLLLFLLWPLFLIIAILIKLNSKGPVYFKQIRVGLNEQ
                     HFKIYKFRTMFLKAEQEGQLTVGGRDARITNVGYYLRKYKLDELPQLMNVFLGHMSLV
                     GPRPEVPYYVSFYSEMQRKVFSIRPGITDNASIAFTNENDLLAQAKNPEWEYINVIMP
                     AKLAINLQYIEHHNLWVDLKIIYRTVAKVWM"
     gene            complement(1014..1901)
                     /locus_tag="PLO59_00565"
     CDS             complement(1014..1901)
                     /locus_tag="PLO59_00565"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HQU99610.1"
                     /translation="MSLELKDVCQATILNVGCTLNRFRWIPKNIELINGYSTPVTLDN
                     EINKNFKGVMLAPYANRVNHAQYFHNKLHTLNKVWPDEDFAIHGFVYNKLFEIGESNL
                     TDEYVSQTFIYSYQGDESGYPYPFNLSVTYCLHIDGRLTCKTAITNMYNETIPFILGW
                     HPLFFNVDAINRLKLNFDSVYKIELDDNLLPIGSSSYNKYKQAKFIEDDFDYCFLLNS
                     NVITITDEYNGIELNIILENGSPSFKYIQIYTPPNKKSIAIEPMTSWPDAFNNKNDLL
                     IIEPQQTVALAYSINCKTL"
     gene            complement(1975..3021)
                     /locus_tag="PLO59_00570"
     CDS             complement(1975..3021)
                     /locus_tag="PLO59_00570"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HQU99611.1"
                     /translation="MALNFQSILSAYGINNQLEVSPFGTGLIHHTYIVGKQPQFILQQ
                     FNQHVFKNPSEVYQNVYEANQYLIQNKAPIKLIIQKLTHNKQPFFISENSYWRLFDFE
                     KNSFSNTKVVYASQAYQAANQFGKLTYHLSGIEINKIKHTIPNFHNLEFRYNQFLQAL
                     KQSKPNNECKTVIEFYKSQSALLNAYYKITSDNYLPIRIMHNDTKISNVLFHEKTNDA
                     ITVVDLDTLMPGYFFNDLGDMIRTYVSTTTEESDNSAPVSMNPEYFKALWHGYKDGLQ
                     NSLTQNEQNAAQYAGPILIYMIGLRFITDYLNNNRYFKINYPLHNLSRAINQMQLLQC
                     YYEHKPQLNDILNA"
     gene            complement(3012..3866)
                     /locus_tag="PLO59_00575"
     CDS             complement(3012..3866)
                     /locus_tag="PLO59_00575"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HQU99612.1"
                     /translation="MKKSLIILAAGKGQRFGGPKQLALVFESYTILDFTIYAAIKAGF
                     NHFIFVINETIETAFKQHWYSGKLPLSCAIDFVLQDNQPQILKPHIVANTAVKGTAYA
                     ILCACHLTEKKFGVLNADDLYGLQALQLLHDGLFGNDAGIAVLYALEKTMSKQGSVNR
                     AKCTVVNGELKNVTEQSYHFESGVIKDSTAMAVPDNTPVSMNLWGFPDESIPLLLNRF
                     DMFLKNPVNEFLLPDFVSYCQNKFMFKALLTDETCLGLTYATDLISLQQQIIDLVKQE
                     KYPQKLWL"
     gene            3994..>4860
                     /locus_tag="PLO59_00580"
     CDS             3994..>4860
                     /locus_tag="PLO59_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQU99613.1"
                     /translation="MNHNRNNHLPIIIMGAFFFIFGFATWLNGTLIPYLKIACELSNF
                     QSYFVATAFYISYFVMALPAAWVLKRTGLKNGMMVGLLVMACGAFIFIPAAYTRTYPL
                     FLTGLFVIGTGLSLLQTASNPYVTILGPIESAAKRISIMGICNKVAGTLAPIILSAII
                     LSDADLITNSLTTMSETDKGPFLDALAARVVKPYTIMGFLLMALAVMVQFSSLPKLNY
                     ETADYDKSATDKRTSVFQFPHLIYGVIALFLYVGVEVMAGDTISNFGTDQGIPLSQSK
                     NFTAFTLSAMIIG"
BASE COUNT         1527 a          791 c          841 g         1701 t
ORIGIN      
        1 aacgttgcca aagcgaaagg ccatctgtaa attttacaat gaggccttaa gcaaatacaa
       61 ttgggctgta ttacccacac aacaactggc agatattgaa agcagttacc atttgtatcc
      121 tttacgaatt aaaaacataa acgaggcaca acgagattta attattcaaa aaatatttga
      181 tgccgatgtt tcagttaacg tgcattttat tccactgcct atgctttcat actataaaaa
      241 tttgggttat gatataaacc attaccctaa tacatatcaa aattacgcca gcgaaatttc
      301 attacctgtt ttttttgata tcaccgaagc acagttaaca tcggttataa acgcagttgt
      361 aaatgctgtt actgaaatat taaaacttga ttgattttga aaaggttaat ggatattacg
      421 gttgctatgc ttttactttt tttattgtgg cctttatttt taataatagc tatactgata
      481 aagcttaatt caaaaggacc tgtttatttc aaacaaatac gggtgggctt aaatgaacaa
      541 cattttaaaa tttataagtt ccgtacaatg tttttgaaag ccgagcaaga aggccagtta
      601 actgttggtg gccgagatgc gcgcataact aatgttggct actatctgcg caagtataag
      661 ttagatgaat taccacaact aatgaatgtg tttttaggcc acatgagttt agtaggccca
      721 agacccgaag tgccttatta tgtttcgttt tatagtgaaa tgcaacgaaa ggtttttagt
      781 atcagaccag gcattactga caatgcctca attgcattta caaatgaaaa tgatttgtta
      841 gcgcaagcaa aaaatccgga atgggagtat ataaacgtaa ttatgccggc taaactggcc
      901 ataaatctgc aatatatcga acaccataat ttatgggttg atttaaaaat aatttatcga
      961 actgttgcta aagtttggat gtaataacaa gtagcaatag gtttaaaatt tcgttataag
     1021 gttttgcagt ttatactata tgccaaagca accgtttgct gtggttcaat tattagcaaa
     1081 tcatttttat tattgaatgc atcgggccag ctagtcattg gttctatagc tatactcttt
     1141 ttatttggcg gtgtgtatat ttgaatatat ttaaacgatg gcgatccgtt ttccaaaata
     1201 atatttagtt caataccatt atattcatcg gtaatggtaa ttacattact attcagtaaa
     1261 aaacaataat caaagtcgtc ttcaataaac tttgcttgtt tgtatttatt atatgaggac
     1321 gagcctatgg gtaataaatt atcatccaat tcaattttat aaacactgtc gaaattcaat
     1381 tttaagcgat taattgcatc aacattaaag aataggggat gccagcccaa tataaaggga
     1441 atggtttcat tatacatatt ggttatggct gttttgcagg taagcctgcc atctatatgt
     1501 aaacaatatg ttacggataa attaaacgga taaggataac ctgattcatc tccttgatat
     1561 gagtaaataa aagtttgcga aacatattcg tcagttaaat tgctttcgcc aatttcaaaa
     1621 agtttgttgt acacgaatcc gtgtatggca aagtcctcat caggccaaac tttgtttaag
     1681 gtatgcaact tattgtggaa gtattgggca tggtttaccc gattggcata cggagcaagc
     1741 ataacgcctt taaagttttt atttatttca ttgtctaaag taacgggtgt gctataccca
     1801 tttatcagtt caatgttttt tggtatccac ctaaaccggt ttaaagtaca acccacattt
     1861 aaaatggtag cctggcatac atcttttaat tctaagctca cgcttgtata atttccaaaa
     1921 ggcactttgc tgatttgaaa tagcatgaat ggttatttta tattttaata aatcctaagc
     1981 gtttaaaata tcatttagtt ggggcttatg ctcatagtaa cattgtagca actgcatctg
     2041 attaattgcc ctgcttaaat tatgcaatgg ataatttatt ttaaagtaac ggttattatt
     2101 taaatagtct gtaataaaac gcaagccaat catataaatt aaaatgggcc ctgcatattg
     2161 agctgcgttt tgttcatttt gtgttagcga attttgcaat ccatctttat aaccatgcca
     2221 caaagcctta aaatactctg ggttcatact taccggtgca ctattatcgc tctcttctgt
     2281 tgttgtactc acataagttc taatcatatc acctaaatca ttaaagaaat aaccgggcat
     2341 caaggtgtct aaatcaacta cggtaattgc atcatttgtt ttttcatgaa aaagcacgtt
     2401 gctaatttta gtgtcattat gcattatgcg aatagggagg tagttatctg aagttatttt
     2461 gtagtaagcg tttagtagcg ctgattgtga tttataaaat tcaataacag ttttacattc
     2521 attgttaggt tttgattgtt ttagcgcctg taaaaattga ttgtatcgaa attctaagtt
     2581 gtgaaagttg ggtatagtat gttttatttt attgatttcg atgccactaa ggtgatatgt
     2641 aagtttaccg aactgatttg ctgcctgata tgcttgtgat gcataaacaa ctttggtatt
     2701 agaaaacgag tttttttcga aatcaaataa acgccaataa ctattttctg atatgaaaaa
     2761 aggttgcttg ttatgggtta acttctgaat aattaatttt atgggagcct tgttttgtat
     2821 taaatattga tttgcctcat atacattttg atacacttct gatggatttt taaaaacatg
     2881 ctgattaaat tgttgtaata taaattgagg ttgcttacct acaatatatg tatgatgtat
     2941 taggcctgta ccgaatggtg atacctctaa ttggttatta ataccatatg cacttaatat
     3001 tgattgaaaa tttaaagcca taatttttgc ggatattttt cctgttttac caaatcaata
     3061 atttgttgtt gtagggatat taaatcggtt gcgtacgtta accccaaaca ggtttcatca
     3121 gttagcaatg ctttaaacat aaatttattt tgacaataag atacaaagtc tggtaagaga
     3181 aattcgttta ccggattttt taaaaacata tcgaatctat ttagtaaaag tggaattgat
     3241 tcatccggaa agccccataa attcatgctt acaggtgtgt tgtcaggtac tgccattgcc
     3301 gtactatctt taataacgcc actttcaaaa tgataacttt gttcggtaac attttttaat
     3361 tcgccattaa ctaccgtgca ttttgccctg tttacactac cttgtttcga catggttttt
     3421 tctaacgcat acagcacagc aatccctgca tcgttaccaa ataatccatc gtgtaataat
     3481 tgcaaagctt gtaatccata aaggtcatcg gcattaagca caccaaattt tttttctgtt
     3541 aaatggcaag cacaaagtat tgcataagca gttcctttaa ctgctgtatt agctacaatg
     3601 tgtggtttta aaatttgagg ttggttatct tgcaaaacaa aatcaatagc acatgatagg
     3661 ggcagtttac ctgaatacca atgttgctta aaggctgttt ctattgtttc atttattaca
     3721 aaaatgaagt gattaaaccc tgctttaatt gctgcataaa ttgtaaagtc gagtatggta
     3781 taactttcga aaactaaggc taactgtttg ggcccgccaa aacgctgacc tttacctgct
     3841 gctaaaatga taagactttt tttcatgctg ttttattaat caggagctaa acaaaatgtg
     3901 ctgaaatatg cattaatatt actcatatga aaacattaaa gtattattgg cggcttttta
     3961 aatttacact tacggtattt taatacacct aaaatgaatc ataacagaaa caaccatttg
     4021 cctataatta ttatgggtgc attttttttc attttcggat ttgctacctg gctcaacggt
     4081 acattaatac cctatttaaa aattgcctgt gagcttagca attttcaatc gtattttgtt
     4141 gcaacagcct tttacatatc ctactttgta atggcattac cggcagcctg ggttttaaaa
     4201 cgtaccggat taaaaaacgg aatgatggta ggtttattgg ttatggcttg tggtgcattt
     4261 atatttatac ctgcagcata cacgcgtaca tatccgctct ttttaacagg cttgtttgta
     4321 attggcacag gcttatcact gttacaaaca gcttcaaatc cttacgttac aattttaggc
     4381 cctatcgaaa gtgcagccaa gcggattagt attatgggaa tttgcaataa agtagcaggt
     4441 actttggcac ccattatttt aagtgctatc attttaagcg atgctgattt gataaccaac
     4501 tcattaacta caatgagcga aacagacaaa ggcccatttt tagatgcctt ggcagcacgt
     4561 gtagttaaac cttataccat tatgggtttc ctattgatgg ctttggcagt aatggttcaa
     4621 ttttctagtt taccaaagtt aaattacgaa acagccgatt atgataaatc ggccacagat
     4681 aaaagaacat cggtgtttca gttcccacat ttaatttatg gtgtaattgc tttgtttctt
     4741 tacgttggcg ttgaagtaat ggcaggcgat accataagta attttggtac cgatcaaggc
     4801 attccgttat ctcaatcaaa aaactttact gcttttacct taagtgcaat gattattggt
//
LOCUS       DAOMAA010000017         4793 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_11163_length_4793_cov_3.226678, whole genome shotgun sequence.
ACCESSION   DAOMAA010000017 DAOMAA010000000
VERSION     DAOMAA010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4793
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11163_length_4793_cov_3.226678"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..1001)
                     /gene="pfkA"
                     /locus_tag="PLO59_00585"
     CDS             complement(18..1001)
                     /gene="pfkA"
                     /locus_tag="PLO59_00585"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008237450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HQU99614.1"
                     /translation="MNNVIEKIGVFTSGGDAPGMNAAIRAVVRTAIYYHKQVEGILMG
                     YEGMIDAQFIPMHARSVSNIIHRGGTILKTARSENFKTKAGRQLAYDNLRKQGIDALV
                     AIGGDGTFTGAEIFYGEFGIPMVGIPGTIDNDLYGTDFTLGYDTAINTAIEAIDKIRD
                     TAASHSRLFFVEVMGRDSGCIALASGIGGGAEYVLMPERSTSINDLIQVLEEGAENKK
                     SSSIVIVAEGEHGGGAYKIAEKVKARFDHYDTKVVVLGHIQRGGSPTAADRLLGSRLG
                     VAAVESLLQGESRKMVGLVNNKVAFTPFADATTKDFILDKELIRISELLAI"
     gene            1165..1857
                     /locus_tag="PLO59_00590"
     CDS             1165..1857
                     /locus_tag="PLO59_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HQU99615.1"
                     /translation="MKHKPTDYDNTFSIDNVIFGFDEGSLKVLLIERAEEAFIGKMAL
                     PGNLVRHDENLDLGASRILYELTGLKDAHLEQLYTFGDINRHPLGRVITVAYYSLIRI
                     KKYKLQPKISFAKRAAWIPVAEIPQLAFDHNVIVEKALDRLRGKLRYKPIGFELLSEK
                     FSLSQLQDLYESILLIAIDKRNFRKKILSYDFLIALNEKQKDVSHRAAKLYRFDKKRY
                     ELLDKKGINFEL"
     gene            1955..2731
                     /locus_tag="PLO59_00595"
     CDS             1955..2731
                     /locus_tag="PLO59_00595"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HQU99616.1"
                     /translation="MKKIYVTLFTAVAMAASSQAQTLITFKVDMSSATLGANTGAGCP
                     APAFDPAIDVVEIMGGDVNGWSAPENVNCGAPFTPNSAVDFVETPAGSLMYERTLNIA
                     NFSNAGDSPYKFRINHSWDNDELRGVNDGNRHIAVTPGTTYIAEQVFNVDGVTLTAIS
                     GVNDLASTFSMTLAPNVIKTSAKFMLALTKSYNVNVKVYDIMGKEVKTIFASKLNGGN
                     YEFVWNGDNNNGIQLNNGCYFYSVTLDGVTAKTEKVYIVK"
     gene            2887..>4793
                     /locus_tag="PLO59_00600"
     CDS             2887..>4793
                     /locus_tag="PLO59_00600"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HQU99617.1"
                     /translation="MIRKIITLCFCLVIYQQQAVAQQPVTWHPFFATDTDSITVYYNA
                     SQGNAALTGVTPIYAHTGVITNLSTSLTDWRYVKTGWAVNTPATTLTPLGNNIFSITF
                     HVRNYYGVPAGETILKVAYVFRNSTGSIVGRNANGSDIYVPIYQPGLNVAIKEPATGG
                     GNTMIVANGSNIAFTGLASQNANLSLSLNGNLLSQVNNTDSLGYTIVAGTTGNNTVHF
                     SGTDGITTKTDSFSFAVNPPLNVQAMPVNVKNGIIYINDSTVILNLFAPNKNFVYVIG
                     SFNNWQLNPNYYMNRDPDGKNYWLQINGLTPGQEYTFQYNVDAAVKIGDPYCDKVVDP
                     WNDFSIDNQTYPNKTPYPTGLTTGIVSVLQTAQTPYVWNVTNFVRPPKEKLVVYELLV
                     RDFISKHNYQTLIDTLHYLKNLGINAIELMPVSEFEGNLSWGYNPNYFFAVDKYYGTK
                     NKFKEFVDVCHANGIAVIMDVVLNHCFGTSPFAMLYWDATNNRPATNSPWLNPIPKHD
                     FNVGSDFNHDSPDTRAFVDRFNSYWLTEYKIDGYRFDLAKGFTQTNSLGNVGFWGQYD
                     ASRVYNITRMCQHIDSVSTGAYKILELFADNSEETVYANQGIMVWGNHNYNYTEAVMG
                     YVGNSNFSWIS"
BASE COUNT         1526 a          953 c          850 g         1464 t
ORIGIN      
        1 ttcgcttcat tcataaatta aatagctaac agttctgaaa tacgaataag ttctttatcc
       61 agaataaaat ctttagtggt ggcatcggca aaaggtgtaa atgcaacttt attattaacc
      121 aagccaacca ttttacggct ttcgccttgt aacaacgatt caacagcggc aacacccaac
      181 ctacttccta ataatctatc ggctgctgtg gggctgccac ctctttgtat atggcccaat
      241 accactacct tggtatcata atggtcgaaa cgagctttta ctttttcggc aattttatat
      301 gcaccaccac catgctcgcc ttcagctaca ataacaatgc ttgacgattt tttgttttca
      361 gcaccttctt ctaatacttg tattaagtcg tttatggaag tactccgttc gggcataagt
      421 acatactcag caccaccacc aatacccgaa gccaaggcaa tacaacccga gtcacgcccc
      481 attacttcaa caaaaaacaa cctactgtgc gatgcagctg tatcgcgtat tttatcaatg
      541 gcttcaatag ctgtattgat agctgtatca taacccaatg taaaatccgt accatataaa
      601 tcgttgtcaa ttgtaccggg tatgccaacc atgggtatgc caaactcacc ataaaatatt
      661 tcggccccgg taaaagttcc atcaccacct atggccacca aagcatctat accttgtttg
      721 cgtaaattat cataagcaag ctgcctgcct gctttggttt taaaattttc gcttcttgct
      781 gtttttagta tggtacctcc tctatgtata atattactca cactacgcgc atgcatgggt
      841 ataaactggg catcaatcat gccttcataa cccattaaaa taccttccac ttgtttatga
      901 taataaatag cggtgcgcac tacagccctt atggctgcat tcatacccgg agcatcgcca
      961 ccagaagtaa acacgccaat tttttctata acattattca tagcttattt tataaaataa
     1021 ttttggagtc agttaataat tacttcctat tattacgcca aacatagtgt atttattaca
     1081 ctaagtttaa tccgaataaa aaatttgcaa ctatcagcag atgccggtta cgttaaatta
     1141 attagttata cataagttag ttatatgaaa cataagccta ccgattatga caataccttt
     1201 agtattgata atgtaatttt tggttttgat gaaggcagtt taaaggtatt actcatcgaa
     1261 agagccgaag aagcatttat tggcaaaatg gcattgcccg gcaacctggt gcgccacgat
     1321 gaaaatttag acttaggcgc atcgcgtata ttgtatgaat taacaggatt aaaagatgcg
     1381 catttagaac aactttacac ctttggtgat attaaccgtc atccattagg ccgcgtaatt
     1441 acagttgcat actactcgct tattcgtata aaaaaataca agctgcaacc taaaatttcg
     1501 tttgccaaac gtgccgcctg gataccggta gcggaaatac cacaacttgc tttcgaccat
     1561 aatgttattg ttgaaaaggc tttagaccgg ctacgtggca aattgcgtta caagccaatt
     1621 gggtttgagt tactgagcga aaaattttca ctatcacaat tgcaggattt gtacgaaagc
     1681 atcttgttga ttgctattga taaaagaaat ttcagaaaaa aaatattgag ttatgatttt
     1741 ctgattgcct taaacgaaaa acaaaaagat gtatcgcacc gtgctgcaaa attgtatcgg
     1801 ttcgataaaa aacgctatga actattagat aaaaaaggta taaactttga actttaaaaa
     1861 attgcaaaac tttatttgga agtaaaatat acttagtgtt atttttacac catgttatta
     1921 ttaataattt atttaaacca ttaatccatt tcagatgaaa aaaatttacg taacactttt
     1981 tacagcagtt gctatggctg catcaagcca ggcacaaaca ttaattacat ttaaagtaga
     2041 tatgagcagt gctactttag gtgccaacac aggtgcaggt tgcccggctc ctgcttttga
     2101 cccggctatt gatgtggttg aaattatggg cggtgatgtt aacggatgga gcgctcctga
     2161 aaatgttaac tgcggtgcac cctttacacc caattcggca gttgattttg ttgaaacacc
     2221 ggcaggctcg cttatgtatg aacgcacttt aaatattgcc aactttagta atgcaggcga
     2281 tagtccttac aaattccgta ttaatcactc atgggataat gatgagttgc gtggcgttaa
     2341 cgatggcaac cgtcatattg ccgttacacc cggtactact tacattgctg agcaagtatt
     2401 taatgtggat ggtgttacat taactgctat ttcaggtgtt aacgatttag catctacatt
     2461 tagcatgaca ttagcaccaa acgttattaa aacttcggct aagtttatgt tggcacttac
     2521 aaaatcttat aatgtaaatg tgaaagttta tgacataatg ggtaaagaag ttaaaaccat
     2581 ttttgcttct aaattaaatg gtggtaacta tgaatttgta tggaatggcg ataacaacaa
     2641 cggcatacaa ttaaataacg gatgttactt ttactcagta actcttgatg gtgttactgc
     2701 aaaaaccgaa aaagtttaca tcgtaaaata atcaatgttt tcatgctttt catccgcggt
     2761 aggactacgg tcttgccgct ttttttttct taaagcaatt ataaatttag gtacaaaaca
     2821 atcctgaaaa tatttattta ttagattcaa aattatagtc aacgtttaca taattcaaaa
     2881 aataaaatga tacgaaaaat tatcacacta tgcttttgct tagttatata tcagcaacaa
     2941 gctgtggcac aacaacctgt tacctggcat cctttttttg caaccgacac cgattcgatt
     3001 acagtttatt acaatgcatc gcaaggcaat gctgctctaa ccggagtaac gcccatttac
     3061 gcgcatacag gtgtgattac gaatttaagt accagcttaa ccgattggcg ttatgtaaaa
     3121 accggatggg ctgtaaatac acccgctaca actttaactc cattaggcaa caatatattc
     3181 agcatcactt ttcacgtacg taattattat ggtgtgcccg caggcgaaac catattaaaa
     3241 gttgcctatg tatttagaaa cagcaccggc agtattgtag gccgtaatgc caatggcagt
     3301 gatatttatg tacccattta ccaacccggt ttaaacgtgg ctattaaaga acccgcaacc
     3361 ggtggcggca ataccatgat tgttgccaat ggaagcaata ttgcatttac aggtcttgcc
     3421 tcacaaaatg caaacctatc gttaagctta aacggtaact tgttaagtca ggttaataat
     3481 accgattcgt taggttatac cattgtggct ggtacaacag gtaataatac cgttcacttt
     3541 tcaggtaccg atggcataac aacaaaaaca gattcgtttt cgtttgctgt aaatccacca
     3601 ctaaatgtgc aagccatgcc ggtcaatgtg aaaaatggaa ttatctacat taacgactca
     3661 accgttattt taaatctgtt tgcacccaat aaaaattttg tatatgtaat aggcagtttc
     3721 aataactggc aactcaatcc gaattattac atgaaccgcg accctgatgg caaaaactat
     3781 tggttacaaa taaatggctt aacacccggg caggaataca cgtttcaata caacgttgat
     3841 gctgcagtta aaattggcga cccttattgc gataaagtag ttgacccctg gaatgacttt
     3901 agtattgaca accaaaccta tcccaacaaa acaccttacc caacaggttt aacaacaggc
     3961 attgttagcg ttttacaaac tgcacaaact ccctatgtat ggaatgttac caactttgta
     4021 cgcccgccaa aagaaaagtt agtggtatat gaattattgg tgcgtgattt tattagcaaa
     4081 cacaattatc aaacattaat agatacacta cactacttaa aaaatttagg aattaatgcc
     4141 attgagctta tgcctgttag cgaatttgaa ggtaatttaa gttggggtta taacccaaac
     4201 tatttttttg ctgtagataa atactatggc acaaaaaata aatttaaaga gtttgttgac
     4261 gtatgccatg ccaacggcat tgctgttatt atggatgttg tacttaatca ctgctttggc
     4321 acctcaccct ttgcaatgtt atattgggat gcaaccaata accgacccgc aaccaatagc
     4381 ccttggttaa accccatacc gaaacacgac tttaatgtag gcagcgactt taatcacgac
     4441 tcgcccgata cacgtgcctt tgttgatcgt tttaacagct actggcttac tgaatataaa
     4501 attgatggct atcgttttga tttagctaaa ggctttacac aaaccaactc attgggcaac
     4561 gttggatttt ggggccagta tgatgcatcg cgtgtttaca atattacccg catgtgtcaa
     4621 cacattgata gcgttagtac aggcgcgtat aaaattttag aattatttgc cgataacagc
     4681 gaagaaaccg tttatgccaa tcaaggtatt atggtttggg gtaatcataa ttacaactat
     4741 acagaagcgg taatgggcta tgtgggtaat agtaatttta gctggatatc gta
//
LOCUS       DAOMAA010000018         4772 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_11239_length_4772_cov_3.725885, whole genome shotgun sequence.
ACCESSION   DAOMAA010000018 DAOMAA010000000
VERSION     DAOMAA010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4772
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11239_length_4772_cov_3.725885"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            325..1416
                     /locus_tag="PLO59_00605"
     CDS             325..1416
                     /locus_tag="PLO59_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99618.1"
                     /translation="MALHDGNYNQAVNLAIKKLKHNSTNENEIEILVTAYEKAQQADL
                     KNIENLKATGDPNNYEKIFDRYHQIDLRQQKVKALPPLIDAGDTVIFAMVNVTAGIEK
                     SREKAAAYFYAQAKQLLATKSKTDARQAYYHLLKLKQYYIEYRDVDSLITHAKQSGTS
                     NVWLCVQNQTQFPLPAYFKTTLLPEDVSALNTEWINYHLQKQNINYDFIINIDVTTLE
                     IGPELVSQINYTDEKQISINETLTNTANNAVTDSSGKVVTVAKTKIITCSVNQTLQQQ
                     KANALVLVNYMQGATTLKVMDVAGGYIWENSYALAYGDLQAASVATINLINNHYKPFL
                     PEEDMLMACAFQLKEQIWQALLNNKYLIK"
     gene            complement(1469..3307)
                     /locus_tag="PLO59_00610"
     CDS             complement(1469..3307)
                     /locus_tag="PLO59_00610"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU99619.1"
                     /translation="MKKVLLILFCCISILKTHAATVYASTYGYTTTDATAALQAAINS
                     GNTPIIVDVQAADWNIGPIKIDGKNPITIIFKPGVVVRALPGMFPNNTDCLFEIKNSQ
                     NVVITAYGASFIMNKSEYTSGEKRHAFAVQNSKKVTITGALLQDAGGDGIYIGADYNL
                     SIKCSETITVTDVVTQNCLREGITITDAKAVKIQHCEFNNSNGATYASGIKLQPVTAD
                     DSLVNIGITRCRMINNQNHAIEVDLQNLTNTSRPVSVTFFRCYSVGNGAEQVKVLGRE
                     SNGVNGNVTLNMCLIDSSNAGGLYIRKNHNEFALNLTECVFRNVAKSAQSNVYPMVSE
                     VTSYTNNVGRHGGVNFNNVLLTYNGNKPWFKSLDNAGTSPGLGSLSGFTVVWNPQQNS
                     APDFGANPSNITVNDSLLAVWPTITVITFSNDNVAGEGNTNRANFKIERTHSNNLYFP
                     MCATYSISGTAINGQDCNRLVGCQIMPTGEPYLKDSLYAMADGITEPNETVIYTLDPS
                     WMYVIGAVPSSTLNIKANSKRDEGAIFETQESNVFYNNITQQLMFKNNDVIWHKLELF
                     NMAGQQMLSNGFASTISLQDFNPGVYVVVLSDANQQIIERTKILKQ"
     gene            3478..4509
                     /locus_tag="PLO59_00615"
     CDS             3478..4509
                     /locus_tag="PLO59_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004156522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HQU99620.1"
                     /translation="MRLHQWAYLDAVNFLSKFTFKRGYNAWLIWLSFYVSKFRGKAIH
                     WGLPVSVSIEPTTSCNLRCPECPSGLRQFTRPTGMLAMDDYQMVVNQLHKQLWYLIFY
                     FQGEPYLNKNLFEMIKYAVQHKIYTATSTNGHYLTDENAKATVTSGLDRLIISIDGLT
                     QEVYEQYRIGGALNKVIEGTKRLVAWKQKLKSKTPHIIFQFLVVKPNEHQINELYQLA
                     NTLGVNEVKLKTAQVYDYKHGNKLIPSIEKYARYVRAADGTYYLKNEMINHCWKMWHS
                     CVITWNGTVVPCCFDKDATHPLGNLKNQSFKTLWQSASYNQFRQDVIGTRSNIDICAN
                     CSEGTKVWV"
BASE COUNT         1550 a          891 c          860 g         1471 t
ORIGIN      
        1 gctaaaattt gtgcactgct taatgcctgc atctgcccct gtacggttgg cagtatcaaa
       61 aaacatatta tggaaaataa gcatgcaata cgggtgcaac atggtttcat atatctttta
      121 aaaaaggagg tgttttgcaa atgaatcatt tttagttatg cactttcgtt taattaatgt
      181 taatcgaaaa cagtttcggt acattaagct taaaaaaaat gtaaggtcat tgcattcaca
      241 tcatagttta ttcaatttta ccgcatgaaa atattttgcc tcgtattttt ttgttcgatt
      301 tttttgcatt gcactacttc aaaaatggct ttgcatgatg gaaattacaa tcaggctgta
      361 aacctggcca taaaaaaact gaagcataac agtaccaatg aaaacgaaat tgaaatatta
      421 gttactgctt atgaaaaagc acaacaagct gatttaaaaa atatcgaaaa tctgaaagcc
      481 acaggcgacc ccaataatta tgaaaaaatt ttcgaccgct atcatcaaat agatttacga
      541 cagcaaaagg taaaggcatt gcctccactt attgatgccg gtgataccgt aatttttgca
      601 atggtaaatg taacagccgg tatcgaaaaa tcgcgcgaaa aagctgctgc ttatttttat
      661 gcacaagcaa aacaattgct tgcaactaaa agtaaaaccg atgcccggca agcttattat
      721 catttattga aattaaaaca atactacatt gaatatcgtg atgttgattc gcttattacc
      781 catgcaaagc aaagtggcac cagtaatgta tggctgtgtg tacaaaatca aacgcagttt
      841 ccgttaccgg catactttaa aactacctta ttgcctgaag atgtatcggc actaaatacc
      901 gaatggatta attatcacct tcaaaaacaa aatatcaatt atgatttcat tataaacatt
      961 gatgtaacta cgttagaaat tggccccgaa ttggtatcac aaataaatta tacagacgaa
     1021 aaacaaattt caattaatga aacgcttacc aatacagcaa acaatgccgt aaccgacagt
     1081 agtggaaagg ttgttaccgt tgcaaaaaca aaaattataa cttgtagtgt aaaccaaact
     1141 ttacagcaac aaaaagcaaa tgctttggtt ttggtaaatt acatgcaagg ggctactacc
     1201 ttaaaagtta tggatgtagc tggtggctat atttgggaaa acagctatgc attagcctat
     1261 ggcgatttgc aagcagcaag tgttgcaacc attaatctga ttaacaatca ttacaaacca
     1321 tttttgcccg aagaagatat gttgatggct tgtgcatttc aacttaaaga acaaatttgg
     1381 caagcgttgt taaacaacaa ataccttatt aaataaaaat gcaggctctg ttttagcctg
     1441 cattaatacc tgttttaatg attacgaatt attgcttcaa aattttagta cgttcaataa
     1501 tttgttgatt ggcatcagat aacacaacta cgtaaacacc ggggttaaaa tcttgtaatg
     1561 atatggtaga tgcaaaacca ttggataaca tttgctgccc tgccatattg aataactcca
     1621 gtttatgcca gattacatcg ttgtttttaa acatcagttg ttgtgttata ttattataaa
     1681 acacattgct ttcctgtgtt tcgaaaatgg caccttcatc gcgcttcgag tttgctttaa
     1741 tatttaaagt tgatgatggc acagcaccta taacatacat ccacgatgga tcgagtgtat
     1801 aaattaccgt ttcattaggt tcggtaatac catcggccat ggcatagagc gaatctttta
     1861 aatagggttc accggttggc ataatctggc atcctaccaa acggttgcag tcctggccat
     1921 ttattgcagt acctgatatg ctatacgtag cacacatagg gaagtacaaa ttgttgctat
     1981 gcgtacgttc aattttaaaa ttagcacggt ttgtattgcc ttcaccggct acattatcgt
     2041 tactaaaggt tataaccgta atggttggcc acaccgctaa taaactgtcg ttaactgtaa
     2101 tgttcgaagg atttgcacca aaatcgggcg cgctgttttg ttgcggattc caaaccactg
     2161 taaagccact taaactacct aagcccggtg atgtacctgc attgtctaat gatttaaacc
     2221 aaggtttatt gccattatac gtaagtaata cattattaaa attaacacca ccgtgcctgc
     2281 ccacattgtt ggtataactt gttacttccg acaccatggg atatacattt gactgtgcac
     2341 tttttgccac atttctaaaa acacattcgg taaggtttaa agcaaattca ttgtggtttt
     2401 ttcttatgta taaaccacct gcattactgc tgtcaattaa acacatgtta agcgtaacat
     2461 ttccgtttac gccattgctt tcgcggccaa gtaccttaac ttgttcggca ccattgccca
     2521 cactataaca tctgaaaaat gtaaccgata caggcctgct tgtattggta agattttgca
     2581 aatcaacttc gatggcatgg ttctgattat ttatcatacg acaacgtgta atacctatgt
     2641 taaccaaact atcgtcagcc gttacgggtt gtagtttaat acctgaagca tatgttgcgc
     2701 cattgctgtt attaaactcg cagtgttgta tcttaaccgc tttagcatcg gttatggtaa
     2761 taccctcacg caaacaattt tgagttacca catcggtaac ggtaattgtt tcgctacact
     2821 taatactcaa attataatcg gcaccaatgt aaattccatc gccaccggca tcttgtaaaa
     2881 gtgcacctgt aatggttact tttttcgagt tttgcacggc aaatgcatgg cgtttttcgc
     2941 ccgatgtgta ttcggattta ttcataataa atgaagcacc ataagcagtt attaccacat
     3001 tttgcgagtt ttttatttcg aataaacaat cagtattgtt tggaaacatg cccggtaatg
     3061 cacgtactac cacaccgggt ttaaaaatga ttgtaatcgg gtttttaccg tcaattttta
     3121 tggggccaat attccagtcg gctgcttgca catcaacaat aatgggcgtg ttgcccgagt
     3181 taattgctgc ttgtaaagct gcagttgcat cggttgttgt gtagccgtaa gtagatgcat
     3241 aaacggttgc tgcatgtgtt tttaaaatac ttatacagca aaataagatg agtagtactt
     3301 ttttcatttg tggggcaagt tgttaaattg ttagggtgag tgttgtgttt tgtatggcaa
     3361 aatacaaaac cggcaattca acatctatgc caaaaagtgg gcagcacttc ggatttaaaa
     3421 ctttaacatt tagcccacaa cattaaaatt aaggtttaat aaatctattt ttgttgtatg
     3481 agattacacc aatgggccta tttagatgct gttaactttt taagcaagtt tacttttaaa
     3541 cgcggctata atgcatggct tatttggctg agcttttatg tttcaaaatt taggggcaag
     3601 gctatacatt ggggtttacc tgtaagcgta agtattgaac caactaccag ttgcaaccta
     3661 aggtgccccg agtgccccag tggtttacgc cagtttacaa ggcctaccgg aatgttagct
     3721 atggatgatt accaaatggt agtaaaccaa ttgcataaac aattatggta tttgatattt
     3781 tattttcaag gcgagcctta tctgaataaa aatctttttg aaatgataaa gtatgccgtg
     3841 caacataaaa tttatacagc taccagcacc aatggtcatt acttaaccga tgaaaatgcg
     3901 aaagctaccg taacctcggg attagaccgc cttataatca gtattgatgg acttacgcaa
     3961 gaagtgtatg agcaatacag aataggcggt gctttaaata aggtaattga aggtacaaaa
     4021 cgattggtag cctggaaaca aaaattaaag tcgaaaacac cgcatatcat ttttcagttt
     4081 ttagtggtta agcctaatga acatcagatt aatgaattat accaacttgc caacacatta
     4141 ggcgttaacg aagtaaaatt aaaaacagca caggtgtatg attacaagca tggcaataag
     4201 ttaataccaa gcatcgaaaa atatgcacgg tatgtacggg ctgctgatgg cacttattac
     4261 ttaaaaaacg aaatgataaa tcattgctgg aaaatgtggc acagttgtgt aataacctgg
     4321 aatggaacag tggtgccttg ctgttttgat aaagatgcca cacacccttt aggcaattta
     4381 aaaaaccaaa gttttaaaac cctttggcaa agcgcttcct acaatcaatt caggcaagac
     4441 gtaataggta cacgcagtaa tattgatata tgtgcaaatt gctcggaagg tacaaaggta
     4501 tgggtgtaat atttttctgg caacagtaat taggcattag tagttagtgg ttagtaatta
     4561 gtggttagtg gttagctttt accaattaac ctttacaaga caactaacca ttaacccccc
     4621 ttgcaactct caactccatt tctatcttta ccaccacaaa aaccaagttt cccttaaccc
     4681 tttaatttta tctattatga aaaatttgtc ctttattaaa aaatcacaca cacacacaca
     4741 cacacacaca caacaaaaat cagcagttgt gc
//
LOCUS       DAOMAA010000019         4703 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_11533_length_4703_cov_3.133821, whole genome shotgun sequence.
ACCESSION   DAOMAA010000019 DAOMAA010000000
VERSION     DAOMAA010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4703
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11533_length_4703_cov_3.133821"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="PLO59_00620"
     CDS             complement(<1..532)
                     /locus_tag="PLO59_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HQU99621.1"
                     /translation="MQAIRNIAIIAHVDHGKTTLVDKILHSTSVFRENQATGELILDN
                     NDLERERGITITSKNVSVTYKGTKINIIDTPGHADFGGEVERVLNMADGVCLLVDAFE
                     GPMPQTRFVLQKAIDLGLKPCVVINKVDKENCTPEEVHEKVFDLMFELGAEEWQLDFP
                     TVYGSAKNNWMSDDWKN"
     gene            812..1603
                     /locus_tag="PLO59_00625"
     CDS             812..1603
                     /locus_tag="PLO59_00625"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQU99622.1"
                     /translation="MSFQSVNQDWEGLAKQDAMWAILTNPDKAGNKWQSNDFFKSGLT
                     EIQRVFSFLNENKIRCIDNNKALDFGCGVGRLTHALATHFDVVDGIDVSATMVEKAEK
                     LKAQFNNNTYFFVNQNTTTAYTDNTFSCIYTSIVLQHIPQPQQGQYIAEFTRILKPGG
                     LLIFQIPTKDIRNLSWFQKIKIKLQLRKRLAQLGIGTYHHMQMNPLPTTQIIEILNKG
                     NCQILQHVYTNHTDIDFGGNLKFIDGRASKDYESSFFVVQKKIQN"
     gene            1619..2560
                     /locus_tag="PLO59_00630"
     CDS             1619..2560
                     /locus_tag="PLO59_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxD N-terminal domain-containing protein"
                     /protein_id="HQU99623.1"
                     /translation="MKLKQPLAITDAAKLINATIIGNTDLHITGINEIHMVQPGDLTF
                     VDHPKYYDKALQSAATFVLINKEVTCPPGKALLFSDDPFRDYNFLTRHFSPFEFNQQN
                     ISSSAKIGSNCYIFPTAIIGNNVVIGNNCTIHAGVVIYDNCIIGNDVIIHANTTIGSD
                     AFYYKKRATGYDKMHTCGRVVIDNMVEIGANCTIVKGVSGDTKIGAGTKTDCHVHVGH
                     DTVIGKNCLFAAGVMIAGVTVIEDDVILWGQVGVQKDLVLAKGTVVLGQSGLGKNTEP
                     GKQYFGSPAIDGREKMKELAWIKRIPEIWEMLQALKK"
     gene            2785..>4703
                     /gene="gyrB"
                     /locus_tag="PLO59_00635"
     CDS             2785..>4703
                     /gene="gyrB"
                     /locus_tag="PLO59_00635"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HQU99624.1"
                     /translation="MSLSEGAVSTAPKSNYGADSIQVLEGLEAVRKRPAMYIGDIGVK
                     GLHHLVYEVVDNSIDEALAGYCKNIAVFINEDNSITVSDDGRGIPTDMHPKEKKSALE
                     VVMTVLHAGGKFDKDSYKVSGGLHGVGVSCVNALSTMLRAEVHRQGKIFMQEYSCGIP
                     KHEVKVIGETNRTGTTVTFLPDASIFITTTYIFDTIAARMRELSFLNKGISITLTDLR
                     EKDEQGNSQKHTFYSERGLVEFVEFLDKTRENLINPPIYIASDKTGIPVEVAMTYNSS
                     YNENVHSYVNNINTHEGGTHLSGFRRALTRTLKSYADKSKMLDKVKIEISGDDFREGL
                     TAVISVKVQEPQFEGQTKTKLGNNEVMGAVDQAVGDMLSAYLEENPREAKTIVNKVIL
                     AATARHAAKKAREMVQRKNVMTGSGMPGKLSDCSETDPEACEIFLVEGDSAGGTAKQG
                     RDRRYQAILPLRGKILNVEKAMEHKIYDNEEIRNMFTALGVSKGTAEDSMALNLTKLR
                     YHKIVIMTDADIDGSHITTLILTFFFRHMKELIEQGFIYIASPPLYLVKKGKEEEYCW
                     TDEQRMLAVKRIAGPDGKESSVNIQRYKGLGEMNAEQLWLTTMNPEQRTLKQVTIESA
                     AEADRIFSMLMGDDVP"
BASE COUNT         1566 a          879 c          928 g         1330 t
ORIGIN      
        1 ggtttttcca atcgtcagac atccaattat ttttggctga accataaacg gttggaaaat
       61 ccaactgcca ttcttctgca cctaattcaa acattaagtc gaaaactttt tcatgaactt
      121 cttctggagt acagttttct ttatctactt tattaataac cacacaaggt tttaatccta
      181 agtcaattgc tttttgtaat acaaaacgcg tttgaggcat tggcccttca aaagcatcta
      241 ctaaaagaca aacaccatca gccatattaa gtacacgctc tacttcacca ccaaaatcgg
      301 cgtgaccagg agtatcaata atgttgattt ttgtgccttt ataagttacc gaaacatttt
      361 tagaagtaat ggtaattcca cgttcgcgtt ctaaatcatt attatccaga attaactcgc
      421 ctgttgcctg attttcgcga aaaacactgg tactgtgtaa aatcttatct accaaggtgg
      481 ttttaccatg gtcaacgtgg gcaataatgg caatgtttct tatagcttgc atatttatgt
      541 tttattaaaa tcgaaatgag gcaaactttg acatcaggtt atctaataag tgtaacttga
      601 aacacggcca atttttgcgg gcgcaaaggt aattttttat agcatataat tatctgactg
      661 ctttaattaa aagttaagca acacctaaac cgctaacaag ctgttcccgg ttttgggtta
      721 acaggttttt agcaaaagct tcgatacttt acttatatcc ctacatcttt aaactgcata
      781 gtttattagc tttgcacgct aatttttaaa aatgagtttt caatcggtaa atcaagactg
      841 ggagggttta gctaaacaag atgccatgtg ggcaatctta acaaatcctg ataaagcagg
      901 caataaatgg cagtctaacg atttttttaa aagcggactt acagaaatac aacgcgtatt
      961 ttcgttttta aacgaaaaca aaataagatg tatagacaat aataaagcac ttgactttgg
     1021 ctgcggtgta ggtcgcctta cacatgcatt ggccacacat tttgatgtgg tagatggtat
     1081 tgacgtatcg gcaacaatgg tagaaaaagc tgaaaaacta aaggcacagt ttaacaacaa
     1141 cacttatttt tttgtaaacc aaaacactac tactgcatat accgataata ctttttcatg
     1201 tatttataca agcattgtgt tgcaacacat accgcagccg caacaagggc aatacatagc
     1261 tgagtttaca cgcatattaa agccaggtgg gttattaatt tttcagatac caacaaagga
     1321 tattagaaac ttatcatggt ttcaaaaaat taaaatcaaa ttacaattac gcaaacgact
     1381 ggcacaactt ggcataggca cttatcacca catgcaaatg aatccgttgc ccacaaccca
     1441 gattattgaa atactcaata aaggtaattg ccaaatatta caacatgttt atactaatca
     1501 taccgatatt gatttcggag gcaatttaaa gtttattgat ggtagagcga gtaaagatta
     1561 tgaaagcagt ttctttgtag tacagaaaaa aatccaaaat taaattcaaa cccaggctat
     1621 gaaactaaag caaccgttag ccattaccga tgcagccaaa cttattaatg caaccattat
     1681 tggtaatact gatttgcata ttacaggcat taacgaaata cacatggtgc agccgggcga
     1741 ccttactttt gttgatcatc caaaatatta cgataaagca ttgcaatcgg cagcaacatt
     1801 tgttttaata aataaagagg taacatgccc acctggcaaa gctttgcttt ttagtgatga
     1861 tccgtttaga gactacaact ttttaacacg ccatttttcg ccctttgaat ttaaccaaca
     1921 aaacattagt tcatcagcaa aaataggcag taactgttat atttttccaa cggccattat
     1981 tggcaacaat gtggttattg gtaataattg taccatacat gcaggtgtgg taatatatga
     2041 caattgtatc attggcaatg atgtaattat ccatgccaat accaccattg gcagcgatgc
     2101 attttactat aaaaaaaggg ctaccgggta tgacaaaatg catacatgcg gacgggttgt
     2161 gattgacaac atggtagaaa ttggcgccaa ctgcaccata gtaaaagggg tatcgggtga
     2221 tactaaaatt ggcgcaggca ctaaaaccga ttgccatgta catgtaggcc atgataccgt
     2281 aattggtaaa aactgcttgt ttgctgctgg cgttatgatt gcaggtgtta cagttattga
     2341 ggatgatgta atactttggg ggcaagtagg agtacaaaag gatttggtac tggcaaaagg
     2401 cacagtagtt ttaggccaat caggccttgg caaaaacacc gaaccgggca aacaatattt
     2461 tggcagcccg gccatagatg ggcgcgaaaa aatgaaagaa ttagcctgga ttaaacgcat
     2521 acccgaaata tgggaaatgc tacaagcact gaaaaaataa agcaacaata aatggatggt
     2581 tttacaaacc ttaaacccgc ccatttatat tcacaattaa agcaccgttt gttaaaaagt
     2641 tttaacgctt taacccttta tttataaggg gttttaggct actatttcgt ataattgcat
     2701 gcctaaaaat caacatgcgt tttttaataa aatgccgcct gtttttagca ggctttttta
     2761 aatttcaaca aaaaaagata tcttatgagt ttaagcgaag gtgctgtaag tacagcgcca
     2821 aaaagtaact acggagccga cagcattcag gttttagagg gtttggaggc cgtacgcaag
     2881 cgacctgcca tgtacattgg cgatattggt gttaaagggt tacaccattt ggtttatgaa
     2941 gtggttgaca attcgattga tgaagcactt gcaggatact gcaaaaatat tgctgttttt
     3001 attaacgaag acaacagcat tactgtaagc gatgatggcc gtggcatacc taccgatatg
     3061 cacccgaaag aaaaaaaatc ggcattagaa gttgtaatga cggtactgca tgcaggcggt
     3121 aaatttgaca aagattcgta taaggtttca ggcggtttgc acggtgtagg cgttagttgc
     3181 gttaatgcct tatcgaccat gttgcgtgcc gaagtacacc gccaaggcaa aatatttatg
     3241 caagaatata gttgcggcat tccaaagcat gaggttaagg ttattggcga aacaaaccgc
     3301 accggtacta ctgttacctt tttgcccgat gcatccatat ttattaccac aacttatatt
     3361 tttgatacga ttgcagcacg tatgcgcgag cttagctttt taaataaagg cattagcatt
     3421 acgcttacag atttgcgtga aaaagatgag caaggcaata gccaaaaaca cactttttac
     3481 agtgaacgcg gcttggttga atttgttgaa tttttagata aaacccgcga aaatctgatc
     3541 aacccaccta tttatattgc atccgacaaa accggcattc ctgttgaagt agccatgact
     3601 tacaacagct cctacaatga aaatgtacat agctatgtaa acaacatcaa cacgcatgaa
     3661 gggggtacgc atttatcagg tttcagacgt gcacttacac gcactttaaa aagctatgcc
     3721 gataaatcga aaatgcttga taaagtaaaa attgaaataa gcggtgacga ttttcgtgaa
     3781 ggccttacag cagttatttc ggttaaagtt caggaaccac aatttgaagg acaaaccaaa
     3841 accaagctcg gtaataatga agtaatgggg gccgttgatc aggcagttgg cgatatgctt
     3901 tcggcctatt tagaagaaaa cccccgcgaa gctaaaacca ttgtaaacaa agtaatacta
     3961 gcagcaacag cacgtcatgc agcaaaaaaa gctcgtgaaa tggtgcaacg caaaaacgtg
     4021 atgactggct caggtatgcc gggcaaacta tccgattgca gcgaaaccga ccccgaagca
     4081 tgtgaaatat ttttggttga gggcgactcg gcaggtggta ctgccaaaca agggcgcgat
     4141 agacgctatc aagcgatact accattacgt ggtaagattt taaatgttga aaaagcaatg
     4201 gaacataaaa tttatgataa tgaagagatt agaaacatgt ttaccgcttt aggtgtaagt
     4261 aaaggtacgg ctgaagattc gatggcttta aaccttacta aacttcgcta tcataaaatt
     4321 gtaattatga ccgatgccga tattgatggc agtcatatca caaccttaat tcttaccttc
     4381 ttcttccgcc acatgaaaga gttgattgaa caaggtttta tttacatagc atcgcctcca
     4441 ctttacctgg taaaaaaagg aaaagaggaa gaatattgct ggacagatga acaacgcatg
     4501 ttagccgtta aacgaattgc tggtcccgat ggcaaagaaa gttccgtaaa cattcaacgc
     4561 tataaaggtt tgggcgaaat gaatgctgaa cagctgtggc taactactat gaatccggag
     4621 caacgtactt tgaaacaagt aactattgaa agtgctgctg aagctgatcg tattttctct
     4681 atgttaatgg gcgatgatgt acc
//
LOCUS       DAOMAA010000020         4693 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_11583_length_4693_cov_3.109746, whole genome shotgun sequence.
ACCESSION   DAOMAA010000020 DAOMAA010000000
VERSION     DAOMAA010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4693
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11583_length_4693_cov_3.109746"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="PLO59_00640"
     CDS             complement(<1..283)
                     /locus_tag="PLO59_00640"
                     /inference="COORDINATES: protein motif:HMM:NF020932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HQU99625.1"
                     /translation="MKKHYLIIALAIATTFTACKKDESDANKNNYTDVLNKVSDDVIL
                     ATYSDLSLKAALLKQATTTLETAPTTANLDAAKTAWVNARKPWEQSEGFL"
     gene            complement(319..1566)
                     /locus_tag="PLO59_00645"
     CDS             complement(319..1566)
                     /locus_tag="PLO59_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HQU99626.1"
                     /translation="MRYILGLLLIIGLAQKSGAQTDTTKTDAVQQVWQKFKVSGYGVT
                     NYYNYDWETIPDKRNEVDVERFNFYIYYHFSELIEFKSEIEFEHGGTGVTMSFDPLEE
                     FGEFESEIEKGGEVVLEQMNLLFKLHPHFKIRVGKMRFYMGNAAKLDKPKNYFTAYRP
                     EVENSILPLGWYETGVEFSGDFKLTESTLPAISYKAYVVTGLDNSAFSSAQWVRRGYQ
                     TRFETINANDLAAALRLDYVFTNKSELGFCLYAGNTTGNRPKNDFTLPAWLTFGDIHF
                     TYNVQPIRISAYGMLGHLQNSEALSLANQNLSNNLNVKRTPVGKAAMGVSLEAGYNLL
                     HNFNKTTHQQLFVFGRYDNYDSMYKTEGNISNNPRHQREVITAGINYMPLPNIVFKGH
                     YAMRKLGSGEYERTTAIGFGFDF"
     gene            1856..2764
                     /locus_tag="PLO59_00650"
     CDS             1856..2764
                     /locus_tag="PLO59_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015329502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HQU99627.1"
                     /translation="MSKQIKHALNNVISTQKLIFRQNLKDIFFLAIGVTMASIGLKGF
                     LLPNKFLDGGAMGVSLLLEIITRFDLSLLIVIVNLPFILIGAKQISIPFAVKSALAIF
                     TLAVLVHFIQIPSVTNDKLLISVFGGFFLGAGIGFAMRGGAVIDGTEVLAITVSRKSS
                     LSIGDFIALFNVFLFIAAMALINVETAMYSMLTYLSASKTVDFIINGIEGYIGVMIVS
                     DKSDEIRHMITYNLGRGVTSFKADKGFGSRGEFEDGGKILFCVVTRLEVTKIILEIEK
                     IDQRAFVVQHTISDTKGGMIKKRPMH"
     gene            2777..3715
                     /locus_tag="PLO59_00655"
     CDS             2777..3715
                     /locus_tag="PLO59_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99628.1"
                     /translation="MYTHKLANGFKYYLHLHFKIAWRAIRHFGLHPLLGFLLIVLVVA
                     GAAIAVSIKTNYAPYIFFTTTCYILWMAADIKRIAFIKLIFGSGNFISVRLFENVIIC
                     LPLLLLLFILKCLLVLLLPIIVIVFAVAVTGKSSIATPTPFYNYPFEFIIGFRKNVAT
                     IFILYLLTAISIAYNNYNLGLFALMAHYILCMSFYQDAENPFYVWIYNESPNSFIFKK
                     LCTANILAVVLALPVIVMLLYFTTSFWQTLFFVVASQIFLTTFIVARYASYPQNVQLP
                     AIIILSLCVYQPPLLLFAIPYYFLIARKKLKLLLPC"
     gene            3709..4377
                     /locus_tag="PLO59_00660"
     CDS             3709..4377
                     /locus_tag="PLO59_00660"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQU99629.1"
                     /translation="MLEIKNLIKKYGNQIVLNNLNLKSDKQVIGLIGDNGSGKTTLFK
                     CIAGIETYQGEISQKQSSNYKTGLLLTEPFFMPYLTGYEYLTLLCKARNINPPDFSKS
                     NIFNLPLNQYAANYSTGMKKKLALTGVLIQQNETFIFDEPYNGVDHQGCQLIMAVIEK
                     LKAKGHTILISSHIIDTLTPICDTLFVIKQGTISEEIQHHNFNAFISNLKLQAQKNID
                     VLWE"
     gene            4468..>4693
                     /locus_tag="PLO59_00665"
     CDS             4468..>4693
                     /locus_tag="PLO59_00665"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQU99630.1"
                     /translation="MIRVLLFEDNLDFADSIKELIANSDGMEFCGTYSNCLNAVKNVQ
                     QHKPQVVLMDIDMPGKNGLDGLRSIRATGDD"
BASE COUNT         1488 a          822 c          806 g         1577 t
ORIGIN      
        1 ataaaaaccc ttcgctttgc tcccagggct tacgtgcatt tacccatgca gtttttgcgg
       61 catctaaatt ggctgttgtt ggtgccgttt caagtgtggt tgttgcttgt ttaagcaagg
      121 ctgcttttaa tgataaatca ctgtaggttg ccagtattac atcatcactt actttattta
      181 gtacatcggt atagttattt ttattcgcat cgctttcgtc tttcttacat gcagtaaatg
      241 ttgtggcaat ggccaaagca attatcagat aatgtttttt cataaatgta tattgtatta
      301 tataaatagt aagttgtttt aaaaatcaaa tccaaatcct atagcagtag tacgttcgta
      361 ttcgccacta cctaatttac gcatggcata gtggccctta aaaacgatat tgggtaaagg
      421 catataatta attccggctg ttatcacttc gcgttggtga cgcgggttgt tactgatatt
      481 accttcggtt ttatacatac tgtcataatt atcatagcga ccgaaaacaa acagttgttg
      541 atgggttgtt ttattaaaat tatgaagcag attataaccg gcttctaatg aaacgcccat
      601 tgcagcttta cccaccggag tacgttttac atttaaattg tttgacaaat tttgattggc
      661 caaactcaat gcttccgagt tttgcaaatg ccccagcatg ccataagcac ttatgcgtat
      721 aggctgcacg ttataagtaa agtgaatatc accaaaagtt aaccaggccg gtagtgtaaa
      781 atcgttttta ggtctgttgc cggttgtgtt gcctgcatat aaacaaaatc ccaattctga
      841 tttattggta aatacataat ccaaacgtaa tgctgcagcc aaatcattag cattaatggt
      901 ttcgaatcgg gtttgataac cacgtctcac ccattgtgct gatgaaaaag cactgttatc
      961 caacccggta actacatacg ctttatacga aatagcaggt aatgtacttt cggttaactt
     1021 aaaatcgcca ctaaactcaa caccggtttc ataccaacct aatggaagta tgctgttttc
     1081 aacttcgggg cggtaggccg taaaataatt tttgggctta tctaacttgg ctgcattgcc
     1141 catataaaac cgcatcttac caactcgaat tttaaaatga gggtgtagct taaacaacaa
     1201 attcatctgc tctaaaacca cttccccacc cttctcaatt tcactttcaa actcaccaaa
     1261 ctcttctaac ggatcaaacg acatggtaac accggtaccg ccatgttcga attcaatttc
     1321 agatttaaat tcgataagct cgctaaaatg atagtagatg taaaaattaa aacgctctac
     1381 atcaacctcg tttcttttat caggtatggt ttcccaatcg taattgtaat aattggttac
     1441 gccataaccg ctcaccttaa atttttgcca aacttgttgc actgcatccg tctttgtagt
     1501 atcggtttgt gcacctgact tttgtgctaa acctataatc agcaacaatc ctaaaatata
     1561 acgcacgcaa taataaattt taaatttcgg ccgcgaatta actatttaga attattctaa
     1621 acaagatatt tttcaagaat taataaatca gccataaaaa tttaagcgaa tacattaata
     1681 gtccaaatag gatatttcca tcaggtatag actttatatt gggtcttatg ctttgcacaa
     1741 aaacacttta aaagttatct cattttttgt ggaacagata caaacattat taatttataa
     1801 ttatcatttt gcgcaacaca ggtaagtcct actttataaa ttttgaaatt gtattatgtc
     1861 taaacaaata aaacatgctt taaataatgt tatcagtacc caaaagctga tattccgcca
     1921 aaaccttaaa gacatttttt tcttggcaat aggcgttacc atggcaagca ttggtttaaa
     1981 aggtttttta ttacccaata aatttttaga tggtggtgct atgggtgtgt cactgttgtt
     2041 agagataatt acgcgttttg atttaagttt attaatcgta atagttaacc tgccatttat
     2101 actaattggt gcgaagcaaa tttcaattcc gtttgcggta aaaagcgcat tagccatatt
     2161 cactttagcg gtgttggtac attttattca aatacccagt gtaaccaacg acaagttact
     2221 tatatcggtt tttggtggat tttttttagg tgcaggtatt ggatttgcta tgcgcggtgg
     2281 tgctgtaatt gatggaactg aagtactggc aattactgta agccgaaaaa gcagtttaag
     2341 cattggcgac tttattgcat tgtttaatgt ttttctattt atagcagcaa tggccttgat
     2401 aaatgtagaa acagccatgt atagtatgct cacttattta tcggcatcaa aaacagttga
     2461 ctttataata aatggtatcg aaggctatat tggtgtaatg attgtttcag acaaatcgga
     2521 tgagatacgt cacatgataa cctataattt aggacgaggc gttaccagct ttaaagccga
     2581 caaaggtttt ggctcaagag gtgagtttga agatggtggt aaaattttat tttgtgttgt
     2641 tacccgttta gaggtaacta aaattatttt ggaaatagaa aaaattgatc aacgtgcatt
     2701 tgtggtacaa cataccatta gcgatacaaa aggtggcatg attaaaaaaa gacctatgca
     2761 ctaaatagca aacagtttgt acactcataa attagccaac ggctttaaat attatctgca
     2821 tttgcatttt aaaattgcct ggcgggcaat caggcatttt ggtttgcatc cgcttttagg
     2881 atttttatta attgtacttg ttgttgcagg tgcagcaatt gcagtaagta ttaaaaccaa
     2941 ctatgcgcct tatatatttt ttacaaccac ctgctatata ctttggatgg ctgccgatat
     3001 aaaacgaatt gcttttataa aattaatttt tggctcgggc aatttcatat cagtgaggct
     3061 cttcgaaaat gttataatat gcttgccgct tctgctctta ctatttatat taaaatgctt
     3121 gcttgtttta ttgctgccca ttatagtaat tgtttttgca gttgcagtta ccggtaaaag
     3181 ttcaattgct acgccaactc cattttacaa ttatccgttt gaatttataa ttggatttag
     3241 aaaaaacgtt gcaacaatat ttattttgta tttgctaact gccataagta ttgcttataa
     3301 caattataat ttgggattat ttgcattaat ggcacattac attttatgta tgagctttta
     3361 tcaagatgcc gaaaacccat tctatgtttg gatttataat gaaagtccaa actcatttat
     3421 ttttaaaaag ttgtgtactg ccaatatact tgctgtagta cttgcactac cggttattgt
     3481 aatgctttta tacttcacta catcattttg gcaaacatta ttttttgtgg tggccagtca
     3541 gatatttcta accacgttta ttgtggctcg ttatgcaagc tacccgcaaa acgtgcagtt
     3601 gccggctata attattttgt cgctttgtgt ttatcagcct cctttactgc tatttgctat
     3661 tccctattac tttttaatag cgcgcaaaaa acttaaactg ttgttaccat gttagagatt
     3721 aaaaatttaa taaaaaaata tggcaaccaa atagtgctaa acaatttaaa cttaaaaagc
     3781 gataagcaag taataggttt aattggcgat aatggaagtg gcaaaaccac actatttaaa
     3841 tgcatagccg gtatcgaaac ctatcagggt gaaatctcac aaaaacaaag tagcaattat
     3901 aaaaccggtt tactgttaac cgaacctttt tttatgccat acctaaccgg atatgagtat
     3961 ctgaccttat tatgcaaagc ccgaaatata aatccaccag actttagtaa gtcaaacatt
     4021 ttcaatttgc cgttaaatca atacgctgca aattatagta caggcatgaa aaaaaaactg
     4081 gcattaactg gtgttttaat acaacaaaat gaaaccttta tttttgatga accctacaat
     4141 ggagttgatc atcagggttg tcaattaatt atggctgtaa ttgaaaaatt aaaagcaaag
     4201 gggcatacaa tacttatatc gtcacatata attgatacgc tgacacctat ttgtgacaca
     4261 ctgtttgtta taaaacaagg cactattagt gaagaaattc aacatcataa ttttaatgcc
     4321 tttataagta acttaaagct gcaagcccaa aaaaatatag atgtgctttg ggaataaaag
     4381 tgcagtaact atttcaccta catatttatg ggagtataat caccaattgc ttttaccacc
     4441 tttgtaaaaa gtaacttatt atcaaccatg atacgcgtat tattatttga agacaactta
     4501 gattttgccg acagtattaa agaattaata gctaattcag atggaatgga gttttgtgga
     4561 acgtattcaa attgccttaa tgcagttaaa aatgtacaac agcataaacc ccaagtggtt
     4621 ttaatggaca ttgacatgcc gggcaaaaac ggcttggacg gtttacgcag tatcagagcc
     4681 acaggcgatg atg
//
LOCUS       DAOMAA010000021         4667 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_11696_length_4667_cov_2.844753, whole genome shotgun sequence.
ACCESSION   DAOMAA010000021 DAOMAA010000000
VERSION     DAOMAA010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4667
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11696_length_4667_cov_2.844753"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="PLO59_00670"
     CDS             complement(<1..686)
                     /locus_tag="PLO59_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99631.1"
                     /translation="MNKYAIILILFFAWSCKKNEPDNPFTNNVVPVPADTINPASIAG
                     LHKNIFAVRCAVPGCHDGSFEPDFRTVQSTYSTLVYHKPIKNDSANSFLYRITPYQVN
                     KSWLYERVTTNDAVLGRMPLYSSKLSDIDLKNIENWINNGAPDINNQPTPPLIIINNP
                     PQVKGYVAIDSNNVRLDTVRLNNISYNPFLIPHGLKFTLVFLLEDDSTAIGNLQQNMV
                     KISSVKDDFSS"
     gene            complement(687..1994)
                     /locus_tag="PLO59_00675"
     CDS             complement(687..1994)
                     /locus_tag="PLO59_00675"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HQU99632.1"
                     /translation="MQRRAFIKRSGMAAAGAVALPYILPQGRLFAATGARKANHVVFC
                     LFAGGVRNLESVHMQDGNLMRCLFNGTQTISSDILPGLDPVPASPLPLPLQNYGTLFK
                     EFRYAQGPTGHYNGHATAITGVYTQTDLDIRANPLNPTVFELYRKHNAPQQTALNSWW
                     VSNTLGPYVALNFSNAEGYGAAYGGNFISPLSFIQSGYNSLANPKTFTPSEESIINEM
                     RNFVDQNFNTQFINGAAGVTNAHTEKIALQNFIADSYNKAANGLYLNPWQLPNANVMN
                     SDMFNIFFAEEILKQFKPELLVVNMQDVDICHSNFTQYANNLRKADYAVAHLWNTIQS
                     TPVLANDTIMIIAPEHGRNLNPNSLIDNFGRKALDHTSDNTSREIFCMIVGPPNVVKQ
                     GQVLNQVAGQSIDIVPTIAGILGFDSQIPGGTLPGSFLHQAFF"
     gene            complement(2006..2497)
                     /locus_tag="PLO59_00680"
     CDS             complement(2006..2497)
                     /locus_tag="PLO59_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99633.1"
                     /translation="MNIWGNKIIAIATCILLVSCEKEVTYVYDVNTVTVSAEGSNKPN
                     VKTTAEFISIAYSDLLGKAISNAEQQQLNVAYNSFGDNKLIEDLIIKNLLQKPGAQIP
                     SKASMLVDVDSFISNTYKKFYNRNPNAFEMYFMHKLITTNNTITPTLIYYAFMTSNEY
                     RYY"
     gene            complement(2478..3332)
                     /locus_tag="PLO59_00685"
     CDS             complement(2478..3332)
                     /locus_tag="PLO59_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99634.1"
                     /translation="MGFILLMALFFVACNKDETEIVKNNTAPIDSTINITIVETYINR
                     SYISLLGRKPDSAEFSNAFQTLKQNNTSVAQRNLFIQSIFTKTDYNKQLFDINHIDLL
                     NQVDTNEFEDKIMLFNFLLTDSTYAPFWSVFTLELNRIFNLQNAIAKLQQNQISIQQL
                     HMVMVDNYFYDQINMGALNFVTSLYQHFLRRYPTVQELDAGIDMVNDKPATVFQQNGS
                     NKNDFIFIFFNATDYYEGQVRDLYLRYLFREPTTAEMVTLTNQYKSQKNYKNIQQYIL
                     TSDEYLGK"
     gene            3467..>4667
                     /locus_tag="PLO59_00690"
     CDS             3467..>4667
                     /locus_tag="PLO59_00690"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQU99635.1"
                     /translation="MFKKISTKIKQALLQCQNEWEAAQIRFEMFHKIEPENVAWNNVA
                     TNYGFNSAAYLLALQQSLESNVQCRYIIVYENELPQGIAYFQLINLKDKHLNQILNSR
                     YFESIYNRFNGQLAEINFNDKNSWLCICGNLFVSGPYGFVLQPNLQPLAKNIFQTMVQ
                     LVEASLPKSDKIFATVVKDLPQQNAMTSILQQMGFIDFAIDPDMILDVKKFGSYDTYI
                     QAFTSKYRVRANSALQKLNDVTVCNMDAENLLRYSKPMATLLAQVIVQAPVHLASLKA
                     DYFHNLKCLMPDNFFVTGYFYNGQLVAFTSTILSDGILHAHLVGFEYSLNKKLGLYQN
                     MLYQLVKEAITINACQLHLGRDAMEIKSTIGATPVTQKLFIKFGNSITHFILDSFTHF
                     SQPKPWVM"
BASE COUNT         1430 a          779 c          883 g         1575 t
ORIGIN      
        1 gaactaaaat catctttaac cgaactaatt ttcaccatat tttgttgcag atttccgatt
       61 gctgtcgaat catcttccaa taaaaatacc aacgtaaatt ttaaaccatg tggtattaaa
      121 aatggattgt agctaatatt gtttaaacga accgtatcaa gccgcacatt gttactgtca
      181 atggcaacat atccttttac ttgtggtgga ttattaatga taattaaggg tggggtaggt
      241 tggttattga tgtcgggtgc gccattatta atccagtttt cgatattttt taaatctata
      301 tcactaagct tactgctgta aagcggcata cgtcccaata cggcatcatt ggttgtaaca
      361 cgttcgtaca accaactttt atttacctga tagggtgtta tgcgatataa aaatgaatta
      421 gcactgtcgt ttttaatcgg tttatggtaa accagggtgc tatacgttga ttgaactgtt
      481 cggaagtcgg gttcgaaact accatcatgg cagccgggaa cagcacatct tacagcaaaa
      541 atatttttat gcaaacctgc aattgaagca ggatttattg tatctgcagg tacgggtaca
      601 acattgtttg tgaaaggatt atcgggctcg ttttttttac aactccaagc aaaaaataaa
      661 ataagtatga tggcgtattt attcatttaa aaaaatgctt gatgtaaaaa actaccaggc
      721 aatgtaccac cgggtatttg cgaatcgaaa cctaaaatgc cggcaatggt gggcactata
      781 tctatacttt gacctgccac ctgatttaat acctgacctt gtttaaccac attgggtggc
      841 cctactatca tgcaaaatat ttcgcgcgaa gtattatcgc tggtgtggtc taatgccttc
      901 cgaccgaagt tatcaattaa actatttggg tttaggtttc ggccatgctc gggcgcaatt
      961 atcataatag tatcgtttgc caggaccggt gtactttgta tggtattcca taaatgtgct
     1021 acagcataat cggccttacg caaattgtta gcatactgtg taaagttgct gtggcatata
     1081 tctacatctt gcatgtttac caccaataat tcaggtttaa attgttttaa tatttcttcg
     1141 gcaaaaaata tattaaacat atcactgttc attacattgg cattgggtaa ttgccagggg
     1201 tttaggtata aaccatttgc ggccttatta tatgagtcgg caataaaatt ctgaagcgca
     1261 attttttcag tatgtgcatt ggttactccg gcagcgccat taataaactg tgtattaaaa
     1321 ttctgatcta caaaattgcg catctcatta ataatactct cttcggatgg tgtaaatgtt
     1381 tttgggtttg ctaatgaatt atagcctgac tgaataaaag aaagtggcga aataaagttg
     1441 cctccataag cggcaccata tccttcggca ttactaaaat ttagtgctac ataaggacct
     1501 aaggtattgg atacccacca actgtttaat gcagtttgtt gaggcgcatt gtgtttgcga
     1561 taaagctcga aaacagttgg gtttaatggg ttggcacgta tgtctaaatc ggtttgagta
     1621 taaactccgg ttattgcagt tgcatggccg ttgtaatgac ctgttgggcc ttgtgcataa
     1681 cggaactcct taaaaagtgt accatagttt tgcaacggta atggcagtgg cgatgcaggc
     1741 acagggtcta aaccgggtaa tatatcacta ctaatggttt gtgtgccgtt aaacaagcaa
     1801 cgcattagat tcccgtcttg catgtgtacc gattctaaat tacgaacacc acctgcaaac
     1861 aggcaaaata ccacatgatt ggctttgcgt gcaccggtgg ctgcaaataa gcgaccctgc
     1921 ggtaaaatat aaggtaatgc tacagctccg gctgctgcca taccacttcg ttttataaag
     1981 gctcttcgtt gcacagattt attttttagt agtaacgata ttcattagat gtcataaatg
     2041 cataatagat taacgtaggt gttatcgtgt tattggtggt tattagttta tgcataaaat
     2101 acatctcgaa tgcatttggg tttctgttgt aaaatttttt ataggtattt gaaataaatg
     2161 aatctacatc aaccagcata gatgcttttg atggtatttg cgcaccgggt ttctgcaata
     2221 aatttttaat tatcaaatct tcgataagtt tattatcgcc aaatgagtta taggcaacat
     2281 ttaattgttg ctgctcggca ttcgaaattg ctttgcccaa taaatcgctg taagcaatag
     2341 aaataaattc ggcagttgtt tttacattgg gtttattact gccttcggcc gaaacagtta
     2401 ctgtgtttac atcataaaca taggttacct ctttttcgca ggaaaccaac aggatgcatg
     2461 ttgcaatagc aattatttta tttccccaga tattcatcgc ttgttaaaat atactgttgt
     2521 atgtttttat aatttttttg tgatttatat tgatttgtaa gtgtaaccat ttcggctgtt
     2581 gttggctcgc ggaataaata ccgcagatat aaatcgcgca cctgcccttc gtaataatca
     2641 gttgcattaa agaagataaa aataaaatcg tttttattgc tgccgttttg ctgaaatacg
     2701 gtagcgggtt tgtcgttaac catatcaatg cctgcatcaa gttcttgtac ggttgggtaa
     2761 cgtcttaaaa aatgctgata caaactggta acaaaattta aagcacccat gttaatttgg
     2821 tcgtaaaagt aattgtcaac cataaccatg tgcaattgtt gtatgcttat ttgattttgc
     2881 tgaagtttgg ctattgcgtt ttgcaggtta aagatacgat ttagttctaa cgtaaacacc
     2941 gaccaaaacg gtgcgtaagt tgaatcggta agtaaaaaat taaacaacat aattttgtct
     3001 tcaaactcat tggtatcaac ctggtttagc aagtcaatgt ggtttatgtc aaacagttgt
     3061 ttgttataat cggttttagt aaaaatactt tgaataaata aatttcgttg tgccaccgaa
     3121 gtattgtttt gttttagcgt ttgaaaagca ttactaaact cggcactatc aggtttgcgg
     3181 cctaataaac ttatataact tcggttaata taagtttcaa cgatggtaat gtttatcgta
     3241 ctatcaatcg gtgctgtatt atttttaact atttcggttt cgtctttatt gcaggcaaca
     3301 aaaaacaaag ccattaaaag tataaatccc aaatatcgca tgtaacaata acctaatgtt
     3361 taaactcaaa cgtaattaat ttattttgca taaaatgatt tcgcaatgtt attctttaca
     3421 actgtattta aaatttcgga acgtttgata cgataacata ccgtctatgt ttaaaaagat
     3481 ttctactaaa ataaagcaag ctttgttaca atgtcaaaac gaatgggaag cagcccaaat
     3541 tcgatttgag atgtttcata agattgagcc tgaaaatgtt gcatggaaca atgtggctac
     3601 aaactatgga tttaatagtg ctgcctattt gttggcattg caacaatcgt tagaaagtaa
     3661 tgtgcaatgc aggtatataa ttgtttatga aaatgaattg ccgcagggta ttgcttattt
     3721 tcagcttatt aatttgaagg ataaacacct gaatcaaatt ttaaattcac ggtattttga
     3781 aagtatttac aatcggttta acggtcagtt agccgaaatt aattttaatg ataaaaacag
     3841 ttggctttgt atatgtggta atttatttgt gagtgggcca tacggatttg tgttacaacc
     3901 aaatttacaa ccattggcaa aaaatatttt tcaaaccatg gtgcaattgg ttgaagcgag
     3961 cttgccaaaa tctgataaaa tatttgccac ggtagttaaa gatttaccac aacaaaatgc
     4021 aatgacttct atattacaac aaatgggctt tattgatttt gcaattgatc cggatatgat
     4081 tttggatgtt aaaaaatttg gtagttatga tacctatatt caggcattta cctcaaaata
     4141 cagggtgcgt gcaaatagtg ctttacagaa acttaatgat gtaactgttt gtaacatgga
     4201 tgcagaaaat ttattgcgct acagtaaacc catggctact ttattggcgc aggtaattgt
     4261 acaggctcct gttcatttag ccagtttaaa ggctgattat tttcacaact taaaatgtct
     4321 gatgcctgat aatttttttg ttaccggtta tttttataat ggccagttag ttgcttttac
     4381 atcaacaatt ttaagcgatg gtatattaca tgcacattta gttggttttg aatatagcct
     4441 gaataaaaaa ctgggtttgt atcaaaacat gctataccaa cttgtaaaag aagccattac
     4501 tataaacgcc tgccaattac atttgggccg cgatgctatg gaaattaaaa gtaccattgg
     4561 tgcaacaccg gttacacaaa agctttttat aaaatttgga aatagtatta cgcattttat
     4621 tcttgattcg tttacccatt tttcgcaacc aaagccttgg gttatgc
//
LOCUS       DAOMAA010000022         4598 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_11994_length_4598_cov_3.023993, whole genome shotgun sequence.
ACCESSION   DAOMAA010000022 DAOMAA010000000
VERSION     DAOMAA010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4598
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11994_length_4598_cov_3.023993"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="PLO59_00695"
     CDS             <1..613
                     /locus_tag="PLO59_00695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQU99636.1"
                     /translation="SAQAKAVQVNKNLMNSIIKAPVNGIVNVRYVEVGTYLAAGNKIV
                     EIVDDSKLKLSVNVNEYQIIQLRNGDKVEVTASVYPEATYQGVITFIAAKSDASLNYP
                     VEIEIKNIAGKALKAGMYGTAHFTPETKTASLTIPRSAFQGGINSNLVFINDNGKAKL
                     QKVVAGRVYGNQVEIREGLKDGDQVIITGQINLVDGKAIAVVK"
     gene            651..3842
                     /locus_tag="PLO59_00700"
     CDS             651..3842
                     /locus_tag="PLO59_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQU99637.1"
                     /translation="MKITEISINRPTMVVVVFTILAILGFISYKSLNYELLPKFSTSM
                     VTISTIYPGAGPDEVQNTVTKKIEDAVSSMENIKKLTSRSMEGVSIVTVEMNVGADAN
                     RSLEDAQRKINAMLSDLPSDAKTPTLSKVSIDDLPIITLSATSNLDQRQFYDLMDQRL
                     APRLSRLSGVAQVQLIGGTEREFQINVDAGKLNAQKISLLQLRQIITNANVDYPTGKI
                     QTTDNQIAIRLSGKYADVDMLRNLVIKTSDDGSQVRLKDVADVRDGQKDAEKLARQDQ
                     KETIVVSIIKQSDANAVSVSEEVHKQIEAIEKEYAANNLKLFVASDSSEFTLESANDV
                     VFDLFIAIILVAVVMLLFLHSVRNSLIVMVSIPLSLIITFAGMYVMGYSLNLMSLLAL
                     SLVVGILVDDAIVVLENIYRHMEMGKNKVRAAFDGVKEIGLTVTSITLVIVVVFLPIS
                     LTNSLVSMILRQFCITVVISTLLSLLVSFTVVPWLSSRFGKLEHLTGKNAFQKFILWF
                     ERQLDKFTAWIGNLLQWSLAHKRYVLTGTIVLLAASFMLLTKGYIGGEFIPKGDRGQF
                     IVTIEMPKDAAVEATNQMTMRAENYLAKQDLVKAITTTVGKSSDGGFGSSATPYKSEI
                     TVTMVDEKLRTEPSNMYAAKVKKVLASQLTGAKVTTSAVSIMGTAERASIDLVVTGTV
                     QDSAMAFAKKALIELQKIDGVSDPKLSAEDGNPELHVSLDRDKMASLGVGMEVVGGTM
                     QTAFSGTNQDSKLKFREGDYEYDINLRYDNFNRKNADDVRNIIFVNNKGQNIRLAEFA
                     TITETSGPTILERRDKLSSVNVQAQVIGRPSGTVTTEFTERLKKLDKPAGVNYLFGGD
                     AENQGNAFGTLGLALLLSIIIMYLIMVALYDDYVRPFVVLFSIPLSLIGALLALALTN
                     NTLNIFSILGVIMLIGLVAKNAIMLVDFANQMKEQGKSTHDALMHANKARLRPILMTT
                     IAMVIGMLPIALASGGVAAIKQGLAWVIIGGLISSMFLTLIVVPVIYKIMDSMSARFG
                     WGDINKKKAIRAQMHADNSETTNVTEHALQHAI"
     gene            4307..>4598
                     /locus_tag="PLO59_00705"
     CDS             4307..>4598
                     /locus_tag="PLO59_00705"
                     /inference="COORDINATES: protein motif:HMM:NF023578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase
                     metal-binding domain-containing protein"
                     /protein_id="HQU99638.1"
                     /translation="MQHTLKKNLCFHCGDACSGDSVSLGDKLFCCTGCKLVYEILEEN
                     NLCTYYNLNEQPGVKTSNIISNRYSYLDEISIVQKLISYKNNNQVHVTFQIPD"
BASE COUNT         1486 a          833 c          919 g         1360 t
ORIGIN      
        1 aagtgcgcaa gcaaaagcag tgcaggtaaa taaaaacctg atgaactcca ttattaaggc
       61 acctgttaat ggaatcgtta atgtgcgcta cgttgaagta ggtacatacc ttgctgccgg
      121 caataaaatt gttgaaattg ttgatgactc aaaattaaaa ctgagcgtaa acgtaaacga
      181 gtatcagatt attcaattac gcaatggcga taaagtagaa gtaacagcat cggtatatcc
      241 cgaagcaact taccaaggcg ttataacatt tattgccgca aaaagcgatg catcgttaaa
      301 ctatcctgtt gaaattgaaa ttaaaaacat tgccggcaaa gccttaaaag ccggtatgta
      361 tggcacagca cattttacac ctgaaacaaa aacagcgtca ttaaccatac cacgctcggc
      421 atttcagggt ggtataaaca gcaatcttgt atttattaat gataacggta aagccaaact
      481 tcaaaaggtt gttgccggca gggtttatgg taaccaggtt gagatacgcg aaggactaaa
      541 agatggcgat caggtaatta taaccggaca aattaatttg gtagatggca aagcaattgc
      601 tgttgttaaa taagtaatag tataaatgct taccaaaaaa aatcaagaaa atgaaaatta
      661 ccgaaatatc tataaacagg cctaccatgg tagtagtggt gtttactatc ctggccatac
      721 ttggctttat aagttacaaa tcgcttaact atgagttgct gccaaagttc agcacgtcta
      781 tggttaccat cagcaccata taccccggtg caggccctga cgaagtacaa aacacggtaa
      841 ccaaaaaaat tgaagatgca gtatcctcta tggaaaatat taagaaactt acctcgcgct
      901 cgatggaagg tgtttcgata gtaactgtag agatgaatgt gggtgccgat gccaacagaa
      961 gtttagaaga tgcacaacgt aaaataaatg cgatgctatc tgacctgcct tctgatgcaa
     1021 aaacaccaac gctaagtaaa gtttcgattg atgatttgcc tatcatcact ttatcggcaa
     1081 catcaaactt agaccaacgt cagttttacg atttaatgga tcaacgtttg gcgccacgtt
     1141 tatcacggtt atcaggtgta gcacaagtac aactgattgg cggcaccgag cgcgaatttc
     1201 aaatcaatgt agatgccggc aagcttaatg cacaaaaaat ttcattgctg caattgcgcc
     1261 agattataac caatgccaat gttgactatc ctaccggtaa gattcaaaca accgataatc
     1321 agattgctat tcgtttatcg ggtaaatatg ctgatgtgga tatgctgcgc aacctggtta
     1381 ttaaaaccag cgatgatggc tcacaagtgc gtttaaaaga tgtagccgat gtacgtgacg
     1441 gacaaaagga tgcagaaaaa ctggcaaggc aagatcagaa agaaacaatt gtggtttcta
     1501 ttatcaaaca atctgatgcc aacgcagtat ccgttagcga agaagtgcat aaacaaatag
     1561 aggctataga aaaagaatat gctgccaata acttgaaact atttgtagcc tccgattcgt
     1621 cagagtttac acttgaatcg gctaacgatg tggttttcga tttatttatt gccatcatac
     1681 tggttgctgt agttatgtta ttgtttttac acagtgtgcg caactcgtta atagtaatgg
     1741 tttcgattcc gctatcgctt ataataacct ttgccggtat gtatgtaatg ggctattcgc
     1801 taaacctgat gtcgttactg gcactatcgt tagtggtagg tattttggtt gacgatgcca
     1861 ttgtggtttt agaaaatatt taccgccaca tggagatggg taaaaataaa gtgcgcgcag
     1921 catttgatgg tgtaaaagaa attggcctta ctgttacatc catcaccctg gtaattgtag
     1981 ttgtgttttt gcctatatca ttaaccaaca gtttggtatc aatgatattg cgtcaatttt
     2041 gtataaccgt agttatttca acgttgttaa gtttgctggt gtcgtttaca gtggtgccat
     2101 ggttatcatc acgttttgga aaattagaac acttaaccgg caaaaatgca tttcaaaaat
     2161 ttatactttg gttcgagcgt caactcgata aatttacggc atggattggc aacttattgc
     2221 aatggtcgtt agctcataaa cgttatgtgc ttacaggcac cattgtatta cttgctgcat
     2281 cgtttatgtt gttaaccaaa ggatacattg gcggtgagtt tatacccaaa ggcgaccgtg
     2341 gacaatttat tgtaactatt gaaatgccta aggatgcagc cgttgaagct accaaccaaa
     2401 tgactatgcg tgccgaaaat tatctggcca aacaagatct ggtaaaagct attacaacta
     2461 ctgttggtaa aagcagcgat ggaggttttg gctcatcagc aacaccttat aaatcagaaa
     2521 ttaccgtaac aatggttgat gaaaaattgc gtaccgaacc atcgaatatg tatgcagcca
     2581 aagttaaaaa ggtattggca tcgcaattaa caggcgctaa agtaaccaca tcggctgtaa
     2641 gtataatggg cactgccgag cgtgcttcta ttgatttagt agtaaccgga accgtgcaag
     2701 acagtgctat ggcttttgca aaaaaagcat taatcgaatt acaaaaaatt gacggtgtat
     2761 cagatccaaa actttcggcc gaagatggta accctgagtt gcatgtatcg cttgatcgcg
     2821 ataaaatggc ttcgctgggt gtaggtatgg aggtggttgg tggtactatg caaactgcat
     2881 ttagtggaac caatcaggac agcaagttaa aattccgcga aggcgattat gaatatgata
     2941 tcaacctgcg ctacgataac tttaaccgta aaaatgccga tgatgtacgc aacatcatat
     3001 ttgtaaacaa taaaggacaa aacatacgtt tggccgagtt tgcaaccatt accgaaacat
     3061 cgggccctac catactcgaa cgcagagata aactttcgtc tgtaaacgta caggcacaag
     3121 taattggccg cccttcgggt accgtaacta ctgagtttac cgagcgtttg aaaaagttag
     3181 ataaacctgc cggtgttaat tacctttttg gtggcgatgc cgaaaaccag ggcaatgcat
     3241 ttggtacttt aggattggct ttactgctat caataatcat tatgtattta attatggtgg
     3301 ctttgtatga tgattatgta cgcccgtttg tggtattgtt ctctatacca ctttcgttaa
     3361 taggcgcact gttggcattg gcacttacca acaacacttt aaatatattt agcatactgg
     3421 gtgtaattat gcttattggt ttggttgcta aaaacgcaat tatgttggtt gactttgcca
     3481 atcaaatgaa agaacagggt aaaagtaccc atgatgcatt aatgcatgca aacaaagcac
     3541 gtttgcgccc tatcttaatg actactattg ccatggttat tggtatgctt cctattgctt
     3601 tggcatcggg tggtgtggca gctattaaac aaggcttggc ttgggttatt attggtggtt
     3661 taatttcgtc aatgttttta accttaattg tagtgcctgt tatttataaa attatggata
     3721 gcatgtcggc acgtttcggt tggggcgata taaataagaa aaaggccata cgcgcacaaa
     3781 tgcatgctga taatagtgaa acaactaatg ttactgaaca tgctttgcaa catgctattt
     3841 aaaaattaaa acaactgatt gtttacttac aatcaaaaca aaacgcacta cttaaaatag
     3901 ttaagtagtg cgttttgttt tttcaattgc gttgcagttt tttatatata gcaactgttt
     3961 taagtattac aacacatcaa tgtcgatttt gctataacaa gtattgtttt aaaatattag
     4021 cacacaataa ctgatatccc tttaacgcaa atgcaaaaaa accattaact ttctaatgca
     4081 tccataaata aatttttcaa attaatacgc agtattaagg acgttaccta accaattaca
     4141 tcactcatgc gcattttaat cctttaaatc aatcaggcag caacaataat tgacttaaat
     4201 tttcaaccta actgtttaaa atatgatttt tatcatatag cttcacaatt attatcagcg
     4261 tggcggcaaa ttcatcggct tacttttgta accttacaat taagaaatgc agcatacact
     4321 taaaaaaaat ttatgttttc actgtggcga tgcctgttcc ggagattcgg tttcattggg
     4381 cgataaactt ttttgctgta cgggctgtaa attggtttac gaaatactgg aagaaaacaa
     4441 tttatgtact tactacaatt taaatgagca accaggtgta aaaacaagta atatcatatc
     4501 caaccgttac agctacttag acgaaatttc gatagtacaa aagctgataa gctataaaaa
     4561 taataatcag gttcatgtta cttttcaaat acccgata
//
LOCUS       DAOMAA010000023         4552 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_12224_length_4552_cov_3.100067, whole genome shotgun sequence.
ACCESSION   DAOMAA010000023 DAOMAA010000000
VERSION     DAOMAA010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4552
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12224_length_4552_cov_3.100067"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="PLO59_00710"
     CDS             complement(<1..938)
                     /locus_tag="PLO59_00710"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HQU99639.1"
                     /translation="MKNIRLIVLMVFVISYCKAQVNENFDDGNITANPVWGGDVASFT
                     VNATQQLQSANVTANSLFYISTPSTTAQNGQWEFFVNLKFSTSGANYVDVFLTADAAI
                     MSGTVNGYFVRIGGTPDEISLYRSDAGIATKIIDGVDGRSDLSSNNQIGIKVICDAGL
                     WSLTDSVVSATPLIQTEGSILDLTYTASQYFGISIKQSTSGFFSKHFFDNIYAGPIIT
                     DVTPPQLTATTLAGVQNLLLTFNEVVDKTTAETPANYTVNNGIGAATSAIQQTNTTQV
                     LAGFATPFADSVTYTVTVTNVTDVAGNPIGTATGSFT"
     gene            1049..2038
                     /locus_tag="PLO59_00715"
     CDS             1049..2038
                     /locus_tag="PLO59_00715"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HQU99640.1"
                     /translation="MKIAVVGATGLVGTKMLEVLAERNFNVTELIPVASSNSLGKHIR
                     FKDKDYAVVGMETAIALKPQIALFSAGGSTSLSWAPKFAEVGTTVIDNSSAWRMDATK
                     KLIVPEVNAHVLQAADKIIANPNCSTIQMVVALQPLHKKYELKRIVVSTYQSVTGTGK
                     KAVDQLMNERKGIDGPMAYPYKIDLNALPHIDVFMDNGYTKEEMKMVHETKKIMGDDN
                     IAVTATCVRIPAVGGHSESVNASFKQPVNLDDIRSLLSNAPGVILVDDVQQLAYPMPL
                     HAHNTDAVYVGRLRYDETQENTINMWIVSDNLRKGAATNAIQIAEYLVSKHLV"
     gene            complement(2254..>4552)
                     /locus_tag="PLO59_00720"
     CDS             complement(2254..>4552)
                     /locus_tag="PLO59_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HQU99641.1"
                     /translation="ITSIFLASILFTQTVWGQLALGDWRAHLSYNATIAAVPAGNKIY
                     AASKASVYYYDKADGDIHGMSKVMGLSDFGVVALNANPFNQVVVVGYANANIDLINNG
                     NIINLSDIKRKTILGDKIIRSIAFKDELAYVSCSFGIVVVNTVRNEIKETYYLGPGGT
                     NLDVFGTTTDDTYIYAATKNGIYRASLSDPFIVNSDNWTRIFSSATAGNFNLITRIGS
                     KIVFNHQLSGGDSLWYLFNGSFQSAPFGYANRYLGVSNNQLLAANFFSVLFFDTNMVL
                     QSYIDNASIPAMLPNCAFNDGTNTWIGDEFQGLIRYKGGNNYDFVFPNGPGSSMSAIV
                     SIMNNDLYVGHAVRNNNWQPTYSQAGFSHYDFNTWQTFNGYAPNNPSLKVGDVWDLVT
                     TTVNPNNSKQAFFGSWGKGLLEYNQGNIYHYTNTNSLLEPNQFGAVRVGGVAYDNNGN
                     LWMTNSEKDSVLKVKRANGTWKSFKSKGVDPTSIVMEILVDDYNQKWINHYRKGILVF
                     NDNNTLDNLADDKWRFLTDVAGNGGLSSPSVFCMTKDKDGQIWCGTEKGICVFYAPGQ
                     VFNSTGFDAQRILIQQDGYNQYLLEKEFVQAIAVDGANRKWIGTQAGGVFLMSADGTK
                     QILNFNEINSPLISNNINSIDINPITGEVFFGTALGICSYRGNAIEGEDACNNVMVYP
                     NPVRPDYDGDIAIKGLVSNGQVKITDVSGHLVYETKALGGQAIWDGKNFDGKKVQSGV
                     YLAFCSDDDGKNTCITKMLIIAGKQ"
BASE COUNT         1376 a          927 c          877 g         1372 t
ORIGIN      
        1 gtaaagctgc cggtggctgt acctataggg ttaccggcaa catcggttac attggttaca
       61 gttactgtat aggttacgct gtcggcaaaa ggcgtggcaa aacctgcaag cacctgtgtt
      121 gtatttgttt gttgtatagc cgaggtagct gcaccaatac cattattaac agtataattt
      181 gcaggtgttt cggctgtggt tttatctacc acttcgttaa aagttagcaa taggttttga
      241 acaccggcta atgttgtagc tgtaagttgt ggtggcgtta catcggttat aattgggcct
      301 gcataaatat tatcaaagaa atgtttacta aaaaagccgg atgttgattg cttaatactg
      361 ataccaaagt attgagaagc cgtataggta agatctaaaa tactgccctc ggtttgtata
      421 agcggtgtgg cacttaccac agaatcggta aggctccaca agccggcatc acaaatcact
      481 tttataccta tttgattgtt gctgcttaaa tcgctgcggc catcaacacc atcaataatt
      541 tttgttgcta tacctgcatc gcttctatat aaacttattt catcaggagt accgccaatg
      601 cgcacaaaat aaccgtttac cgtgcctgac attatggctg catcggcagt taaaaacaca
      661 tccacataat tggctcctga tgtagaaaat ttgaggttta caaaaaattc ccactgtccg
      721 ttttgagctg tagtgcttgg cgtgcttata taaaacaagc tgttggctgt aacattagcg
      781 gactgcaatt gctgtgtggc attaactgta aacgatgcta catcgccacc ccaaaccgga
      841 tttgcggtaa tattaccatc gtcaaaattt tcgttaacct gtgccttgca gtagctgatt
      901 acaaaaacca ttaagactat aagtctgata tttttcatgc tcaaaactta gaaattattg
      961 cagtaaaaat agtatttaat gcatgctgca tgttattact ttcgcatgct tcatattggt
     1021 tattaaatta tttaaaaaca catcgctcat gaaaatagct gtagttggcg ctaccggttt
     1081 ggttggcact aaaatgttgg aagtacttgc cgaaagaaat tttaatgtaa cagagcttat
     1141 accggttgcc agttcaaact cattaggaaa acacattcgc tttaaagaca aagactatgc
     1201 agtagttggt atggaaactg ccattgcgct aaaaccccaa atagcactgt ttagtgccgg
     1261 tggcagcacc tcgctatcct gggcacctaa atttgccgag gttggtacaa ctgtaattga
     1321 caactcatcg gcatggcgta tggatgccac caaaaaacta attgtacccg aggtaaatgc
     1381 acatgtacta caagctgccg ataaaattat tgccaacccc aattgcagca ccattcaaat
     1441 ggtagttgcc ttacagccct tacataaaaa gtatgaatta aaacgcattg tggttagtac
     1501 ctatcaaagc gtaaccggca ccggtaaaaa agcggttgac caattaatga atgagcgcaa
     1561 agggatagat ggccccatgg catatcctta taaaattgac cttaatgcat taccacacat
     1621 tgatgtattt atggataatg gttatactaa ggaggagatg aaaatggtgc atgaaaccaa
     1681 aaaaattatg ggcgatgata acattgccgt tacagctacc tgtgtacgca tacctgctgt
     1741 tggtggccat agcgaaagtg taaatgcatc ttttaaacaa cctgttaact tagatgatat
     1801 acgtagtttg ttaagtaatg ccccgggtgt aattcttgtt gatgatgtgc agcagcttgc
     1861 ttaccccatg ccattacatg cacacaatac cgatgcggtt tatgtggggc gcttacgcta
     1921 tgacgaaacg caagaaaata ccataaatat gtggatagtg agcgacaact tacgcaaagg
     1981 cgcagctacc aatgccatac aaattgccga gtatttggta tcgaaacact tggtgtgatt
     2041 ttataaaagc ggcaaggctt tatcaaaatg aaaccgtaaa tttttttcga agtatcaagc
     2101 gttacgctat tgccaaagaa attttttaaa aattgtgggg ttgttactac gtttaatata
     2161 actcaagcgc tttaaattag catattgttg ctgcttgctt accgttttta cttcttttaa
     2221 aattctttaa aaaaagctac ataggcgtaa aagttattgc ttacctgcaa taatcagcat
     2281 tttggttatg cacgtgtttt tgccgtcatc atcgctacaa aaagcaaggt aaacgccact
     2341 ttgaactttt ttaccgtcaa aattttttcc atcccaaatt gcttgcccac ccaatgcttt
     2401 ggtttcgtaa accaaatggc cgcttacatc ggtaatttta acctgtccat tactaaccaa
     2461 tcctttaata gcaatatcgc catcataatc aggccgtacc ggatttggat aaaccattac
     2521 attattacaa gcatcctcac cttcgatagc attacctctg taagaacaaa tacctaaggc
     2581 tgtaccaaaa aaaacttcgc ctgttattgg gtttatatca attgaattga tgttgtttga
     2641 tatgagcggg ctgttgattt cattaaaatt taaaatctgt ttggtgccat cggcactcat
     2701 taaaaatacg ccaccggctt gtgtaccaat ccatttgcgg ttagcgccat caacggcaat
     2761 tgcttgtaca aattcttttt cgagtaaata ctgattgtat ccatcttgct gaattaatat
     2821 tcgttgtgca tcaaagcccg tgctgttaaa cacctgaccg ggtgcataaa aaacacaaat
     2881 gcctttttca gtgccacacc aaatttgtcc gtctttatct ttagtcatac aaaatacact
     2941 ggggctgctt aagccaccat tgccggcaac atcggttaaa aaccgccact tatcatcggc
     3001 caaattatct aaggtgttat tatcattaaa aaccaaaatg ccttttcggt aatgattaat
     3061 ccatttttga ttgtaatcat caaccaatat ttccataact atagaagtgg ggtcaacacc
     3121 ttttgattta aagcttttcc aggtaccatt ggcgcgtttt actttaagta cgctatcttt
     3181 ttcactattg gtcatccata aattaccatt gttatcatag gctacaccgc ccacgcgcac
     3241 cgcaccaaac tgattgggct ctaacaaact gttggtattg gtatagtggt aaatattacc
     3301 ttggttatat tcgagtagcc ctttacccca cgagccaaaa aaagcctgct tgctgttatt
     3361 agggtttacg gtggtagtta ccaagtccca aacatcgcca actttaagcg atgggttatt
     3421 gggcgcatat ccattaaacg tttgccatgt attaaaatcg taatgcgaaa aacctgcctg
     3481 agaataagta ggttgccagt tgttattgcg cactgcatgg ccaacgtata aatcgttatt
     3541 cataatacta actattgcag acatactgga gccgggtccg ttaggaaaaa caaagtcgta
     3601 attattaccg cccttgtatc tgattaatcc ctgaaactca tcgcctatcc acgtgtttgt
     3661 gccatcatta aatgcacagt taggtaacat agccggtatg gatgcattgt caatgtacga
     3721 ttgcaaaacc atgttggtat caaaaaacaa aacagaaaaa aagttggcag ccagcaattg
     3781 attgtttgat acgcctaaat agcggtttgc gtaaccgaat ggtgccgatt gaaaactacc
     3841 gttgaataag taccataacg aatcgccacc ggatagctga tggttaaata caattttact
     3901 gcctatgcgt gtaatcaaat taaaattgcc ggcagtagcc gatgaaaaaa tacgagtcca
     3961 gttatctgag tttacaataa atgggtcgga taacgatgca cggtatatgc cattttttgt
     4021 tgcagcatat atgtacgtat catcagtagt ggtgccaaac acatcgaggt ttgtaccacc
     4081 ggggcctaag tagtacgttt ctttaatttc gtttctaact gtgtttacta caacaatacc
     4141 aaagctgcat gaaacgtatg caagctcatc tttaaaagca atgctgcgta taattttatc
     4201 gcctaaaatg gttttgcgtt taatgtcgct caggttaata atgttgccat tatttattaa
     4261 atcgatattg gcattggcat agcctaccac tactacttgg ttaaaaggat ttgcatttaa
     4321 ggcaacaaca ccaaaatcgg acaggcccat tactttactc atgccatgta tatcgccatc
     4381 ggctttatca taatagtaaa ccgatgcttt gcttgctgca taaattttat tgccggccgg
     4441 cacagcagct atggttgcat tataggataa atgtgcgcgc caatcgccta aagccaattg
     4501 cccccacacg gtttgagtaa aaagaattga tgctaaaaat atagatgtta tt
//
LOCUS       DAOMAA010000024         4539 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_12291_length_4539_cov_2.885816, whole genome shotgun sequence.
ACCESSION   DAOMAA010000024 DAOMAA010000000
VERSION     DAOMAA010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4539
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12291_length_4539_cov_2.885816"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /locus_tag="PLO59_00725"
     CDS             complement(<1..876)
                     /locus_tag="PLO59_00725"
                     /inference="COORDINATES: protein motif:HMM:NF018218.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HQU99642.1"
                     /translation="MTNQQEQKPGNESKKDNPFGKKPGTPKFNPYWIYGVVIILFIGL
                     QFIDWGSKPEEIYWQQFKTSMLDSGDVDKIEIINETRAEITIKKDKLALDKYKKVRNK
                     PFTNTLNEGPHFYMNISKNGTFEKQLEEVQKNYPENQRITVVYKPYERGLMEYVISWI
                     LPLAIIVIIWMVLIRKMGGGAGGSQIFNIGKSKATLFDKDLNVKVTFNDVAGLEEAKV
                     EVMEVVDFLKNPKKYTALGAKIPRGALLVGPPGTGKTLLAKAVAGEAQVPFFSLSGSD
                     FVEMFVGVGASRVRDL"
     gene            complement(918..1421)
                     /gene="rsfS"
                     /locus_tag="PLO59_00730"
     CDS             complement(918..1421)
                     /gene="rsfS"
                     /locus_tag="PLO59_00730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HQU99643.1"
                     /translation="MAVKKTHQATVKNVTKKTAIKKESIRSKSSSVKAPKVASKRSTK
                     GSNKTAKTSTPSLIEVIIAAMADKKAQQITSMDLRAIESASTDYFVIAHAGNSTQVEA
                     IAANVEDEVKKQLQEKALHTEGYQNAEWILLDYFNVVVHVFLEEKRNFYRLEQMWADA
                     DITNHNA"
     gene            complement(1604..2068)
                     /gene="smpB"
                     /locus_tag="PLO59_00735"
     CDS             complement(1604..2068)
                     /gene="smpB"
                     /locus_tag="PLO59_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HQU99644.1"
                     /translation="MKSEKPIILVTNRKASFEYFFLTTYEAGLVLTGTEIKSVRNGEA
                     NLQDAFCFLKDNELWIKNMHIAPYKEGAYNNVDTKRQRKLLLNKNELRKIESKLKEKG
                     TTVVATKLLLNENGFAKLEIAIAKGKKNYDKRQSLKEKDDKRQMAKTLKKII"
     gene            2121..2987
                     /locus_tag="PLO59_00740"
     CDS             2121..2987
                     /locus_tag="PLO59_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HQU99645.1"
                     /translation="MRFIAKALPYFSLVKLSHSVFALPFAAIGFALGLQAQHAMPVTL
                     GIKVVLCMVFARNAAMAFNRWADKYIDAQNSRTSIREIPAGIIKPSAAAWFTLLNSMA
                     FIFTTYFINSACFYLSFVALAVVLGYSYTKRFTPLCHLVLGLGLSLAPIGAYIAVTGV
                     FNFIPLMFSAIVLFWVCGFDIIYALQDIDFDRSQQLKSIPVLLGINGALTFARICHFM
                     CAVLLIATGVYAGFAIWYWVGTALFIALLIYQHLLVKADDFSKINLAFFTTNGIGSLV
                     FGFFVIIDISLS"
     gene            complement(3103..4269)
                     /locus_tag="PLO59_00745"
     CDS             complement(3103..4269)
                     /locus_tag="PLO59_00745"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQU99646.1"
                     /translation="MNQSLFNTQLQNMNSRLVVSKSKSKIYLDKNEQSDDVALNYKEK
                     LAALLIQTNWNRYPSNDLSDIEQNVANYCGLTAQNIALSSGSATLITTLLNYFAINRK
                     SIVITQPSYSLFDYHCKSYNIEYTPWMLDDNLNFNLKTLPALHSNSVLIITAPNNPTG
                     TNISMKQLENILKQNPTALIVLDGVYCEFSKTDFTPLINQYENLMVLRSFSKAFPVAG
                     LRLGYLCASATITANVKKLLLQFAINPFTLLFARYILFDAKFMHDSKQCVKNIISERN
                     RMKTYLTSNFSANQIYVYPSEGNFLLIKIKSNHNKLIQSFSHAGIKVLDTSNFVKLNQ
                     TVRISIGTTSENNAVMRCIEKAMDLRINNQKTSPANISFTNNFNAIRSYAAVCY"
     gene            complement(4319..>4539)
                     /locus_tag="PLO59_00750"
     CDS             complement(4319..>4539)
                     /locus_tag="PLO59_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99647.1"
                     /translation="DILGKAGTDRFEKHEPAIEWIKMLTQSCFEIDEMQFKHVSLNET
                     GLNIVMQPDGFIGFKYDTENILAIIKAI"
BASE COUNT         1219 a          776 c          823 g         1721 t
ORIGIN      
        1 taaatcgcgc acacgcgatg caccaacgcc tacaaacatc tcaacaaaat cagaacctga
       61 cagggagaag aagggaacct gtgcttcgcc tgcaactgct tttgctaata aagttttacc
      121 ggttccgggt gggccaacta atagtgcacc acgtggaatt ttggcgccta aggcagtgta
      181 tttttttgga ttttttaaaa agtcaacaac ttccatcact tccacttttg cttcttcaag
      241 ccctgcaaca tcattaaatg ttacttttac attcaagtct ttgtcgaaca aagttgcctt
      301 gctcttacca atgttaaaaa tctgactgcc accggcacca ccacccattt tgcggattag
      361 taccatccag ataataacaa taattgccaa tggtagtatc caactaataa catattccat
      421 taaaccacgc tcatacggtt tgtacacaac agtaatacgt tgattctcgg ggtagttttt
      481 ttgtacttct tcaagttgtt tttcgaatgt accgttttta ctaatgttca tgtaaaaatg
      541 tgggccctcg ttaagggtat tggtaaatgg cttatttctt actttcttat atttatctaa
      601 agcaagctta tctttcttaa ttgtgatttc ggcccgggtt tcgttaataa tttcaatttt
      661 atctacatca ccactatcaa gcatgctggt tttaaactgt tgccaataaa tttcttccgg
      721 tttgctgccc caatcaataa attgaaggcc aataaaaagt ataattacta caccgtaaat
      781 ccaataaggg ttaaactttg gtgtgcccgg cttttttccg aaagggttgt cttttttcga
      841 ttcattaccc ggcttttgct cctgctggtt tgtcatttct aatctttaat aatctgattt
      901 tatattgtaa tttgttttta ggcattgtgg ttggtaatat cggcatcggc ccacatttgt
      961 tctaaacgat agaagttacg tttttcttct aaaaaaacat gcactacaac gttgaaataa
     1021 tccagtaata tccattcggc attttgataa ccttcggtat gtagggcttt ttcttgtagt
     1081 tgctttttta cctcatcttc aacattggca gctatggctt ctacttgtgt actattacct
     1141 gcatgtgcaa ttacaaagta atcggtgctg gcactttcaa tggcacgcaa atccatactg
     1201 gtaatttgtt gcgctttttt atcggccatg gcggcaataa ttacttcaat taatgaaggg
     1261 gtacttgttt tagctgtttt gttcgaacct ttagttgaac gcttacttgc aacttttggt
     1321 gctttaacgc tgctgctttt agatcttata ctttctttct tgattgctgt ctttttggta
     1381 acgtttttaa ccgttgcctg atgcgttttt tttaccgcca ttttgtgttt tttgataata
     1441 gctgcaaatg tattcttttt tactgcatgc tacattgacg gtacctacgc ggctaaattt
     1501 tgtagctttt cgttttttta gttaaaaaat tgtttaggta tggtgggtag aaaagggcaa
     1561 aactttacgt atgctgtgca tttatgtttg attggatagg ctattaaatt atttttttta
     1621 atgttttagc catttggcgt ttatcgtctt tttcttttaa cgattgacgc ttgtcatagt
     1681 tctttttgcc tttggctatg gcaatttcaa gctttgcaaa accattttca tttaatagaa
     1741 gtttggtagc tactacggta gtcccttttt cttttagttt actttcaatt ttacgcaact
     1801 cgtttttatt caacaataat ttgcgttggc gtttggtgtc aacattgtta taggcgcctt
     1861 ctttataggg agcaatgtgc atgtttttaa tccacaattc attgtctttt aaaaagcaaa
     1921 atgcgtcttg taaattggct tcgccattac gcaccgattt tatttcagta cctgttaata
     1981 ccaaaccggc ttcatacgtt gtcagaaaaa aatattcgaa cgatgctttt ctgttcgtta
     2041 ccaaaataat tggtttttcg gatttcatgc ttgcaaagaa aattctttta acacctttgt
     2101 caaactttag tatttacaat atgcgtttta ttgccaaggc tttgccttat ttttcattag
     2161 taaaacttag ccattcggtt tttgctttac cttttgcagc cataggtttt gcattgggtt
     2221 tgcaagcaca gcatgcaatg cctgtaacat tgggcattaa agtggtgtta tgtatggtgt
     2281 ttgcgcgcaa tgctgccatg gcttttaacc gttgggctga taaatatatt gatgcacaaa
     2341 attcgcgtac aagcattcgc gaaattccgg ctggtattat aaaaccaagt gcagcagctt
     2401 ggtttacctt gcttaatagt atggcattta tttttacaac ttattttatt aatagcgctt
     2461 gcttttattt gtcgtttgtg gcattggctg ttgttttggg ttacagttat accaaacggt
     2521 ttacaccatt atgtcatttg gttttaggtt tggggttaag cctggcaccc attggtgctt
     2581 acattgccgt aacaggcgtt tttaatttta ttccactaat gttttcggca attgttttgt
     2641 tttgggtatg tgggtttgat attatttatg ctttacagga tattgacttt gatagaagtc
     2701 aacaactaaa atcaatacct gttttgttag gaataaacgg tgctttaacc tttgcgcgta
     2761 tttgccattt tatgtgtgct gtattgttaa ttgctacagg tgtatatgca gggtttgcaa
     2821 tttggtattg ggtaggaact gctttgttta tagctctatt aatttaccag cacttattgg
     2881 ttaaagctga tgatttcagt aaaattaatt tggcattttt tacaaccaat ggcattggta
     2941 gtttggtgtt tggttttttt gtgattatag atataagttt atcataaaca gcatctttta
     3001 ctccatacat ttagatgtat tttaataaca gacttattta tccggtttat aatactgcta
     3061 atatttcatt tgcacgaata gcgaactcag cttaaaacac atttaatagc atacggctgc
     3121 ataactacgt atagcgttaa agttatttgt aaacgaaata tttgctggtg atgttttttg
     3181 gttgttaatg cgcaaatcca ttgctttttc aatacatcgc attacggcat tgttttcgga
     3241 tgtggtgcca atacttatac ggacggtttg gttaagtttt acaaagtttg atgtgtctaa
     3301 taccttaatg ccggcatggc taaatgattg aatcaactta ttgtgatttg atttgatttt
     3361 gatgagcaag aagtttcctt ctgatggata aacataaatt tgattagcac taaaattact
     3421 tgttaaatat gttttcatac gattacgttc gctaattatg tttttaacgc attgttttga
     3481 atcgtgcata aattttgcat cgaaaagtat ataacgtgca aacaataatg taaacggatt
     3541 gatggcaaat tgaagtaaca gcttttttac attagccgta atagttgccg atgcgcataa
     3601 ataacctaac cgcaagcctg caaccggaaa tgcttttgaa aacgaacgta gcaccattaa
     3661 attttcatac tgattaatca aaggcgtaaa atctgtttta ctaaattcgc aatacacacc
     3721 atcaagcact atcaaagcag tggggttttg tttaagtatg ttttccaatt gcttcatcga
     3781 aatattggta cctgttggat tattaggtgc agtaataatt agtacggagt ttgaatgtag
     3841 cgcaggcaat gtttttaaat taaaatttaa attgtcatct aacatccagg gcgtatattc
     3901 aatattgtat gatttgcagt gataatcgaa aagcgaatac gaaggctgcg ttattactat
     3961 actttttcgg tttatcgcaa aatagtttaa caaagttgta ataagcgtgg ccgagcctga
     4021 gcttagcgca atattttgag cagttaagcc acaataattt gcaacatttt gctctatatc
     4081 acttaaatcg tttgaaggat agcgattcca atttgtttga attaaaagcg ctgcgagttt
     4141 ttctttataa ttcaatgcaa catcatccga ttgttcgttt ttatcgagat atatttttga
     4201 tttcgacttg gatacaacca ggcggctatt catgttttga agctgtgtat taaataagga
     4261 ctgattcatc agattgtata ttaagtagtg tggttattat ttaaaagtta gtttaaaact
     4321 atatggcttt aattatagcg agtatgtttt cggtatcata tttaaatcca ataaatccat
     4381 ccggttgcat aactatgttt aatccggttt cattcaacga tacgtgctta aattgcattt
     4441 catcaatttc aaagcagctt tgagtaagca tttttatcca ttctattgcc ggttcatgtt
     4501 tttcgaatct atcggttccg gctttgccta aaatatctt
//
LOCUS       DAOMAA010000025         4518 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_12389_length_4518_cov_2.800134, whole genome shotgun sequence.
ACCESSION   DAOMAA010000025 DAOMAA010000000
VERSION     DAOMAA010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4518
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12389_length_4518_cov_2.800134"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1146
                     /gene="sucC"
                     /locus_tag="PLO59_00755"
     CDS             <1..1146
                     /gene="sucC"
                     /locus_tag="PLO59_00755"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003007924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HQU99648.1"
                     /translation="IVAETPEQAVEAAKELQKQTGTGWWVVKAQIHAGGRGKGKIVGS
                     EQRGVALAKSLDDVAAISKNILGNTLVTIQTGDAGKVVNKVLIAQDVYYPGPSEVKEF
                     YMSVLLNRATNRNIIMYSTEGGMSIEDVAHDTPEKIFKEEIDPKVGLQPFQARKIAFN
                     LGVSGNAFKEMVKFVSALYKAYESTDASMFEINPLFKTSDDKIIAVDSKVNIDDNALY
                     RHPDYAAMRDKSEEDPTEVEASEHELNYVKLDGNVGCMVNGAGLAMATMDIIKLAGGD
                     PANFLDVGGTANAARVERAFRIILKDKNVKAILVNIFGGIVRCDRVAQGIVDAYKNIG
                     EIRVPIIIRLQGTNAVEAKKIIDESGLKVYSAIQLKEAAQLVSEVLK"
     gene            1337..2689
                     /locus_tag="PLO59_00760"
     CDS             1337..2689
                     /locus_tag="PLO59_00760"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HQU99649.1"
                     /translation="MEQALTKDEVKALLKQGTDSLDTSQLKALVSSGIQKLNGDEVLH
                     FLIIVSVVLISSRLLGEIFRKFKQPVVVGEILAGIIIGPSLIGTYFPEFFINTFMAQP
                     RAFGAFDGLANVGVILLMFVAGMEVDIQQIRKQGKQASIISLSGIAFPFALGFATIWF
                     FHDYVLSTPNENKLVASMFFGTALSITALAVIVKVLFDLRMIRTRVGGLVLTAAMVDD
                     FLGWILFSIILQMMHSSHSEGTPYSVLIVLGFAVFMVTIGRWIIDKILEYTAKFLSPP
                     GGIITVGICLCFLGAVFTEYLGIRGVFGAFLIGLAVGDSKHFSETTQHVLQQFIINII
                     APLFFASVGLRVNFVTNFDWQVVLIILSIACIAKIVGAGVGSRLSGLTKNESMAIAFG
                     MNARGSQEIVLGMIALQAGIIDEKVFVALVVMTMVTIVAAGPLMRYYMERHLKTQPAA
                     "
     gene            2713..3276
                     /locus_tag="PLO59_00765"
     CDS             2713..3276
                     /locus_tag="PLO59_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018478708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HQU99650.1"
                     /translation="MHHHYFIYKPYGVLSQFKCELPKKKLLGSLYAFAPATMAIGRLD
                     EDSEGLLYLTTDGMVSEKIRSKNVPKEYFVQVDGIITNEALTHLADGVFITINKQQYN
                     TKKCNVYLLESEPVLPKRAKNIRHAKHGPTSWISITLHEGKFRQIRRMTAAVGFPTLR
                     LVRVRIGSFWMPYLDAGQVIEVDQLYY"
     gene            3335..3934
                     /locus_tag="PLO59_00770"
     CDS             3335..3934
                     /locus_tag="PLO59_00770"
                     /inference="COORDINATES: protein motif:HMM:NF019247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 domain-containing protein"
                     /protein_id="HQU99651.1"
                     /translation="MKKPLITLAACLFAANVLWAQTDAKKPEMSKEEMAAMKAWEAFA
                     TPGAVHKMLAKDDGQWDEEITMWYAPGAPPETMKATCVNSMILGGRYQQSVHRGNFQG
                     QPFEGISTLAYDNTRKVFISSWIDNMGTGMMIMEGISKGDNVVAFEGKQTDPVSGKEL
                     KMSEEFIMVDANTQRMIMYQTTAEGKTFKNMEITFKRKK"
     gene            complement(3988..>4518)
                     /locus_tag="PLO59_00775"
     CDS             complement(3988..>4518)
                     /locus_tag="PLO59_00775"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQU99652.1"
                     /translation="AFYGTCFFQLISSIPQYFKTQSHYAEDIIGLLMALNGLLVVLIE
                     MPIISKIEHTKFPMRYIICGVFCLTLSYAALYVHQGLSTAIIYIVIITLSEVFAMPFM
                     MNFTLSRPTKDRKGQYAALYSMAYGLSFIAAPAIGLNIAEQYGFKSLFGVITIASIFL
                     AFGFYSIQKHLQNIRQ"
BASE COUNT         1462 a          741 c          944 g         1371 t
ORIGIN      
        1 atagttgcag aaacaccgga gcaagctgtt gaagcagcta aagaattaca aaaacaaacc
       61 ggcaccggtt ggtgggttgt aaaagcacaa atacatgccg gtggacgtgg caaaggaaaa
      121 atagttggtt cagaacagcg cggtgttgct ttagccaaat cgttagacga tgtagcagct
      181 atttcgaaaa atattttagg caatactttg gtaaccatac aaacaggcga tgccggcaag
      241 gttgtaaaca aggttttaat tgcacaggat gtgtattatc ccggcccttc agaagtaaaa
      301 gagttttaca tgagtgtatt acttaaccgt gctaccaacc gcaacataat tatgtattca
      361 accgaaggcg gaatgagtat tgaagatgtt gcacatgaca cacccgaaaa aatatttaaa
      421 gaagaaatag atcctaaagt tggattacaa ccgtttcagg cgcgcaaaat tgcatttaac
      481 ctgggtgtaa gtggcaatgc atttaaagag atggtaaaat ttgtatcggc tttatacaaa
      541 gcttatgaaa gcaccgatgc atctatgttc gaaataaatc ccttgtttaa aaccagcgat
      601 gacaaaatta tagcggttga ctcgaaggta aacattgatg ataatgcatt gtaccgtcac
      661 ccggattatg cagctatgcg cgataaatca gaagaagatc ctacagaagt tgaagctagc
      721 gaacatgaat taaattatgt aaagttagat ggcaatgtgg gctgtatggt taatggcgca
      781 ggtttggcaa tggctaccat ggatataatt aaacttgcag gtggcgaccc tgctaatttt
      841 ttagatgtag gtggtacagc caatgcagca cgtgttgaac gtgcatttcg tattattttg
      901 aaagacaaaa acgtaaaagc cattttggta aatatttttg gaggcattgt acgttgcgac
      961 agagtagcgc agggaatagt tgatgcctat aaaaatattg gcgagataag agtgccgatt
     1021 ataattcgtt tgcaaggcac caatgcagta gaagcaaaaa agataattga cgaatcgggt
     1081 cttaaggttt attctgccat acaattaaaa gaggcagcac aactggtatc agaagtttta
     1141 aaataagctt attaaaatgt tacggaaccg ttgaattttt attcaacggt ttttttgttc
     1201 atttttttaa tcatcaatta cgaattaaat tgcttagcca aagttcgtaa tgaataacta
     1261 ttcaaaataa agtctactaa ttttgtagaa tgtatgtttt ttgttttatg tttggccgcg
     1321 ttaaaaacaa aactgcgtgg aacaagcact tacaaaagac gaagtaaaag ccctattaaa
     1381 acaaggtacc gatagtttag atacctcaca attaaaagct ttagttagta gtggtattca
     1441 aaaactaaat ggcgatgaag tattgcattt tctgataatt gttagtgtag ttctgatttc
     1501 atcacgctta ttaggcgaaa tttttcgaaa atttaaacag cctgtagttg tgggcgaaat
     1561 attagccggt attattatag gcccaagttt aataggtact tatttccccg agttttttat
     1621 caacaccttt atggcacaac cacgtgcatt tggtgcattt gatggtttgg ctaacgtagg
     1681 tgttatacta ttaatgtttg ttgcaggtat ggaagtagat atacaacaaa tacgcaaaca
     1741 aggtaaacaa gcttccatca taagtttatc aggtattgcc tttccgtttg ctttaggctt
     1801 tgccactatt tggttttttc atgattatgt tttaagcaca cctaatgaaa ataaattggt
     1861 ggcctccatg ttttttggta cagcgctttc cattacagca ttagcagtta ttgtaaaagt
     1921 actttttgat ttaaggatga ttagaacaag agtagggggc ttggttttaa cagcagctat
     1981 ggttgatgat tttttgggat ggatattgtt ttcaatcatt cttcagatga tgcattcatc
     2041 ccattctgaa ggaacaccct acagtgtgtt aattgtttta ggttttgcgg tttttatggt
     2101 aaccatcgga cgatggataa ttgataaaat attagaatac acagcaaagt ttttatcgcc
     2161 acccggtggc attataaccg taggcatttg cctttgtttt ttaggtgccg tgtttacgga
     2221 gtatttgggt atccgtggtg tgtttggtgc ttttttaata ggcttggccg taggcgattc
     2281 gaaacatttt tcagaaacca cacaacacgt tttacaacag tttattatta acatcattgc
     2341 gcccttattc tttgcatcag taggtttacg tgtaaacttt gttaccaatt tcgattggca
     2401 ggttgtactt attattttgt caattgcatg tattgcaaaa attgttggtg caggtgtggg
     2461 cagtcgttta agcggtttaa ccaaaaacga atcaatggca attgcttttg gtatgaatgc
     2521 acgtggttcg caagaaattg ttttgggaat gattgcttta caagcgggta ttattgatga
     2581 aaaagtgttt gttgctttag ttgtaatgac aatggttacc attgttgccg caggcccatt
     2641 gatgcgttat tatatggagc gtcatttaaa aacacaacct gcagcttaac cggtgcaatt
     2701 agcactataa acatgcacca ccattatttt atttataagc cttatggtgt gctcagtcag
     2761 tttaaatgcg aattacccaa aaagaaacta ttgggcagtt tgtatgcttt tgctccggca
     2821 acaatggcta tcggccgatt agacgaagac agcgaagggt tgttatacct aaccactgat
     2881 ggtatggtaa gtgaaaaaat ccgttcgaaa aatgtgccta aagaatattt tgtacaagta
     2941 gatggcataa ttacaaacga agcgcttacg catcttgctg atggggtttt tattactatc
     3001 aataaacaac agtataacac aaaaaaatgt aacgtttatt tattagagtc ggaaccggtt
     3061 ttacctaaac gtgcaaaaaa catcaggcat gccaagcacg gccccaccag ttggataagc
     3121 attaccttac atgaaggaaa gttcaggcaa ataagacgta tgactgccgc tgttgggttt
     3181 ccaactttgc gtttggttcg tgtacgcatt ggctcctttt ggatgcccta cctggatgca
     3241 ggtcaggtaa ttgaagttga tcaattatat tattgaaact tttttattct gaatcaaaag
     3301 gttaagttta ctgcaacatt taaaaactac tattatgaaa aaaccactaa ttactttagc
     3361 agcatgcttg tttgcagcca atgtactttg ggcgcaaaca gatgccaaaa aacctgaaat
     3421 gagcaaagaa gaaatggctg ccatgaaagc atgggaagct tttgctacac caggtgccgt
     3481 acataaaatg ttagccaaag atgatggaca atgggacgaa gaaattacca tgtggtatgc
     3541 acccggtgca cctccggaaa ctatgaaagc tacctgtgta aacagtatga ttttaggtgg
     3601 ccgataccaa caatcagtac atcgcggtaa tttccaaggt cagccgtttg agggtatttc
     3661 aaccttggca tacgataata cgcgtaaggt ttttattagt tcctggattg acaacatggg
     3721 caccggtatg atgattatgg aaggtatttc taaaggcgat aatgtagttg catttgaagg
     3781 caagcaaacc gatccggttt cgggcaaaga attaaaaatg agcgaagagt ttattatggt
     3841 tgatgcaaat acacaacgca tgataatgta tcaaactaca gccgaaggaa aaacttttaa
     3901 aaacatggaa attactttta agagaaagaa ataacagcag ttttgaataa aaaattataa
     3961 aaagcccact tgaaaaaagt gggtttttta ttgcctgatg ttttgtaaat gtttctgaat
     4021 actataaaat ccaaatgcca aaaaaatact ggcaattgta ataacaccaa ataatgattt
     4081 aaagccatat tgttcggcta tatttaaccc aatagcaggc gctgcaataa acgataaacc
     4141 ataagccatg gagtataaag cagcatattg accttttcga tctttggttg ggcggctcaa
     4201 tgtaaagttc atcataaaag gcatggcaaa tacttccgac aaggtgatga ttacaatata
     4261 aattattgcc gtggacaaac cttgatgcac gtataatgcc gcatacgata gtgttaaaca
     4321 aaatacacca caaataatat aacgcatcgg aaatttggtg tgctcaattt tcgaaattat
     4381 gggcatttct attaatacta ccaataagcc atttaaagcc attaaaaggc caattatatc
     4441 ttctgcataa tgcgattggg ttttaaaata ttgcggtatg ctggatatta attgaaaaaa
     4501 acaagtgcca taaaaagc
//
LOCUS       DAOMAA010000026         4495 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_12518_length_4495_cov_2.388964, whole genome shotgun sequence.
ACCESSION   DAOMAA010000026 DAOMAA010000000
VERSION     DAOMAA010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4495
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12518_length_4495_cov_2.388964"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1951)
                     /locus_tag="PLO59_00780"
     CDS             complement(<1..1951)
                     /locus_tag="PLO59_00780"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HYR domain-containing protein"
                     /protein_id="HQU99653.1"
                     /translation="MVRLTNDYAITASAGANGSVSPAGTSLVLAESNQVYTITPNTGY
                     CIADVLVDNSSIGAVSSYTFSNVLTNHTISATFYAQTIYYSDNDSDGYGNPLIDSVAC
                     TQPLGYVNNNTDCNDADSLEHAGQVWYLDADGDQYSSGSSITQCSRPVNGFVASELIA
                     ISGDCNDANGNIHPGTVEVCDGLDNNCNGLTDNNEPGVLDTILPVPTFANLPNITAQC
                     SVTVTSPTANDNCAGTLTATTVDPITYNTQGSFVIHWTYDDGHGNIVTQNQNVIVDDN
                     TIPVISGCLSSITVNASPGQWGSQVNWTPPTASDNCAIDTLISNYNPGAFFTVGTTTV
                     TYTASDVNGNTATCSFNVTVNAPNITGTVNVTIFANDIQFSNSHPNPNTAITVMATVH
                     NYSNVDATNFSCHLVNDFDNTTYPDTTIPLLAAGQEIQISWTITTPATDEFVPMSVWI
                     DYGNALAESNELENHAIRPFINGQPALGGAIKVIANANPNPGYTGQAMTVCGKAWYDN
                     LAQPLPDTSVAGATVMLTINQTGEVYYTTTNSLGNYCFNFAAANDTGNYTYLVSVTDF
                     TLTGDTTGNYNLILSPCLLDLSVSMEVDGSYLCQSVNYIAAGSSITGVVHVQNNCQAI
                     SQTTTLFVTTTGTPIPGTFTIPPLAP"
     gene            complement(1952..3391)
                     /locus_tag="PLO59_00785"
     CDS             complement(1952..3391)
                     /locus_tag="PLO59_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99654.1"
                     /translation="MQFKSIIILCLLSVMSVYRIHAQQPEMLLNWAKTMNNTTPYSGN
                     VYGEACTVDSNGNFYITGEFTGSVDFDPGAGIAALTSAGGLDVFFAKYDVNGNFILAK
                     AIGGNHANGDYVKSISIDKNANIFIGGFFYGTADFDPGTAVVNKTAASQSEGYVAKYD
                     ASGNFVYINLLAGSDYETVYTLATDTNGNVAATGQFYGGLSIITGGGTTTLSSNGAYD
                     AFLVKFDASGNTVFSKHFGNFNNDNGYAVATDAAGNIYVAGVFVGTVDLGAGPISASG
                     GGLNDVFIAKFSAAGVYNYGKVFGGNGNDLCYALDVDDNNNLFLVGYFNNTVDFDPGA
                     GVATRTSGGLKDAYLLKFDNSGSFVHAITMGGTGDDEAYAISVNSISQTFISGYFNSA
                     TADFDPGPSVVNLSAAGGLDAFIAKYDATGSYLYARSFGQNAGDEVGRGITAFGNYNV
                     FATGYFTHVVDFDPEAGVVNLGSVGTQNG"
     gene            3765..>4495
                     /gene="rsmA"
                     /locus_tag="PLO59_00790"
     CDS             3765..>4495
                     /gene="rsmA"
                     /locus_tag="PLO59_00790"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011585013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HQU99655.1"
                     /translation="MTAKIVRAKKHLGQHFLNDEGIAQQIVAALQCNVKFTNVLEIGA
                     GMGVLTKYILKLDAKFYLVEIDHESVAYLESNYPQLSNKIISKDFLKLNLTQIISAPV
                     AIIGNFPYNISTEILFKVLENKDLVPLVVGMFQKEVAMRVTSAPGNKAYGIVSVLIQA
                     YYNVEYLFTVDAQVFTPPPKVQSGVIRLTRNDVIALPCNEMLFSKVVKTAFNQRRKTL
                     RNALKSFNKTGLPFMDKRAEQLSCS"
BASE COUNT         1379 a          917 c          875 g         1324 t
ORIGIN      
        1 cgggtgccaa tggcggaatt gtaaatgtac cgggtatggg cgtaccggtt gttgttacaa
       61 aaagtgtagt agtttgcgat attgcctgac agttattttg aacatgcact acaccggtta
      121 ttgaactacc tgctgcaatg taatttacac tttggcataa gtacgagcca tccacttcca
      181 ttgaaaccga taaatcaagc agacatggcg atagtattaa gttatagttg cctgtggtat
      241 cgcctgttaa tgtaaaatca gttacgctaa caaggtatgt atagttacct gtatcattag
      301 ctgcagcaaa attaaagcag tagttaccaa gcgaatttgt agtggtataa taaacttccc
      361 ctgtttggtt aatggttagc attacggttg ctccggctac tgatgtatct ggtaaaggtt
      421 gtgctaaatt atcataccag gctttgccac aaaccgtcat ggcttgtccg gtataaccgg
      481 ggttgggatt ggcgtttgca attactttta tagcaccgcc caaagcaggc tggccattta
      541 taaatggccg aatggcatgg ttttccagct cattcgactc ggcaagtgca ttgccataat
      601 caatccacac agacattgga acaaattcat cagttgcagg tgtggttatc gtccaactta
      661 tttgaatttc ctgccctgct gcaagtagtg gtatggtagt gtcgggatag gtagtattat
      721 cgaaatcatt aactaaatga cagctaaaat ttgtagcatc tacatttgaa taattatgta
      781 cggtagccat taccgtaata gcggtattgg gattgggatg cgaattacta aactgtatat
      841 catttgcgaa aatagttacg ttaactgttc cggtaatatt tggtgcattt acagttacat
      901 taaatgaaca ggttgctgta ttgccattta catcggatgc agtataggtt actgttgtgg
      961 ttcctactgt aaaaaaagcg cccggattgt agttgctgat taatgtatct atagcgcagt
     1021 tatcgctggc cgttggtggt gtccaattaa cctgactacc ccattgcccg ggtgatgcat
     1081 taacagtaat gcttgacaaa catccgctta ttaccggtat ggtattatca tcaacaatta
     1141 cattttgatt ttgagttact atgtttccat gtccgtcatc ataagtccag tgaatcacaa
     1201 agctaccctg ggtgttatac gtaatcgggt caactgtagt tgctgttaag gtacctgcac
     1261 aattgtcgtt agcagttggg gatgtaacgg taaccgaaca ttgcgcggtt atattgggca
     1321 aatttgcaaa cgttggtaca ggtaaaatgg tatctaatac tccaggttcg ttgttgtcgg
     1381 ttaaaccgtt gcaattatta tccaaaccat cacaaacttc tacagttcct gggtgtatat
     1441 ttccatttgc gtcattacag tcaccgctta tagctattaa ctcacttgca acaaaaccat
     1501 taacgggtcg gctacattgt gtgatactgc tgcccgaact gtattgatcg ccatcggcat
     1561 ccaaatacca aacctgcccg gcatgttcta acgagtcggc atcgttacaa tcggtattat
     1621 tattcacata acccaaaggc tgtgtacatg caaccgagtc aattaacggg ttgccatagc
     1681 catcactatc gttatcggaa taataaatag tttgcgcata gaatgtggct gatatggtgt
     1741 gattggttag tacattagaa aatgtataac tacttacagc gccaatacta ctattatcta
     1801 ccagcacatc ggcaatgcaa taacccgtgt tgggggtaat cgtataaacc tgattgcttt
     1861 cggctaaaac aagtgaagta cctgcaggcg atacactgcc attggcaccg gccgaagcgg
     1921 taatagcata gtcgtttgta agcctaacca tctagccatt ttgagtacct acactaccta
     1981 aattaactac accggcttcg gggtcgaaat ctactacatg ggtaaaatat cctgttgcaa
     2041 acacattata gttaccaaaa gctgttatcc ctcttcctac ttcatcgcca gcattttggc
     2101 caaaactacg tgcatacaaa taactgcctg ttgcatcata ttttgcaata aaagcatcaa
     2161 gacctccggc cgcacttaaa ttaacaaccg atggcccggg gtcaaagtcg gcagttgcac
     2221 tattaaaata tccggatata aaagtctggc ttatactatt aacagaaatg gcataagcct
     2281 cgtcatcacc ggtgccaccc attgttattg catgcacaaa gcttccgctg ttgtcaaact
     2341 ttagtaagta agcatcttta agtccgccac ttgtacgggt tgcaacacct gccccgggat
     2401 caaagtcaac agtgttgtta aaataaccta ccaaaaataa gttgttgtta tcatcaacat
     2461 ccaaggcata gcacaagtca tttccatttc caccaaaaac ttttccgtaa ttataaactc
     2521 cggcagccga aaacttagca ataaaaacat cattaaggcc accacccgaa gctgaaatgg
     2581 gaccggcacc caaatcaacc gtgcctacaa aaacacctgc aacgtaaatg ttaccggcag
     2641 catcggtggc tacggcatag ccgttatcat tattaaaatt accaaagtgt ttcgaaaaaa
     2701 ccgtattgcc cgaagcgtca aactttacca gaaatgcatc atacgcccca ttgcttgata
     2761 acgtggtagt tccaccgcct gttataattg aaagacctcc gtaaaattgg cctgtggctg
     2821 caacattacc attggtatcg gtagccagtg tataaacggt ttcgtaatcg ctgccggcca
     2881 ataaatttat ataaacaaaa tttccggatg catcatattt agctacatat ccttcagatt
     2941 gcgaggccgc agttttatta accactgctg tcccgggatc aaaatcagcg gtaccataaa
     3001 aaaaacctcc aataaatatg ttggcgtttt tatcaatgga aatgcttttt acataatctc
     3061 cattggcatg attgccgcct attgcctttg ccaaaataaa attgccgttt acatcatact
     3121 tggcaaaaaa cacatctaag ccaccggcac ttgtaagggc agcaatacct gcaccgggat
     3181 caaaatcaac ggaaccggta aattcgcctg ttatataaaa attaccgtta ctgtcaaccg
     3241 tacaagcttc gccataaacg ttacctgaat acggtgtggt attgttcatg gtttttgccc
     3301 agtttaataa catttcgggt tgctgtgcat gtatcctgta cactgacata acacttagca
     3361 ggcatagtat gatgattgat ttgaattgca ttgacgcttt aaaaagcatt gcattttggg
     3421 tagggttatt catattatca gagattaggg atggggttat ttttaggaag tgtatgaaaa
     3481 tggtttcgga caataacaaa cacttttaag tatcggctat ttacttatgg ttaaaaatta
     3541 ttttggttga atggttacac aaattatata atagaatttg ccaaaatcaa tccccataaa
     3601 tggggattga ccaagtaatt atatttgtta cagaaaatcc tataaaaaca acatattcag
     3661 caatacgatt tattgcagac aggtatagaa atcaatttgg aatatttttg cagtcaaaca
     3721 ataaacgcta ctgatttctt ttctttacgc aaaataaaaa cagtgtgact gcaaaaattg
     3781 tacgcgccaa aaaacacctg ggccaacatt ttttaaatga cgaaggcatt gcccaacaaa
     3841 tagttgcagc acttcaatgt aacgtaaagt ttaccaacgt attggagatt ggcgcaggta
     3901 tgggtgtact tacaaagtac atattaaagt tagatgcaaa attttattta gtcgaaattg
     3961 atcacgaatc cgttgcttat cttgaaagca attacccaca actgagtaat aaaattatat
     4021 cgaaagattt tttaaaactt aatcttacac aaataatttc agcacctgtt gccattattg
     4081 gcaactttcc ttacaacata tcaaccgaaa tactttttaa agttttagaa aataaagatt
     4141 tggtaccatt agttgttggt atgtttcaaa aagaagttgc catgcgtgta acctcggcac
     4201 cgggcaataa agcctatggt attgtaagtg tgctcataca agcttactat aatgttgaat
     4261 atttgtttac cgttgatgca caggtgttta caccgccacc caaagtacaa agtggcgtta
     4321 tcagattaac acgtaatgat gtaatagcct tgccttgcaa tgagatgcta tttagcaaag
     4381 ttgtaaaaac ggcatttaac cagcgcagaa aaacgttacg taatgcatta aaatcgttta
     4441 acaaaacagg cctcccattt atggataaac gagccgagca acttagctgc agcga
//
LOCUS       DAOMAA010000027         4477 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_12613_length_4477_cov_2.772049, whole genome shotgun sequence.
ACCESSION   DAOMAA010000027 DAOMAA010000000
VERSION     DAOMAA010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4477
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12613_length_4477_cov_2.772049"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1318)
                     /locus_tag="PLO59_00795"
     CDS             complement(<1..1318)
                     /locus_tag="PLO59_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99656.1"
                     /translation="MKRLLFLLLLTSCFLQSSASHLMGGEITWQCLPTGEYRFFMKLY
                     RDCNGVSFPTTGISLDVSNKPGLTSIPMTIISQTDISPDCNANSGVPITCAGATGSTA
                     GAVEEFYFQSNPIILNGVPPAAGWIFSYSNCCRNSAITNLPNAGGLGFTLRAIMYSFN
                     LQNANPCFDSSPFFVESPNTIICSGYPFTYNPTAIDPEIDSLVYSWAQPLDDFTGGAW
                     TASNPAPIGFSPGYSFTSPLPGPAQNPNNVAATLNSTTGEVSYTSFTNGVFVTVNKVS
                     AYKCGIVIAEIFRDLQITLKVCNSLSPGVPNLPPTVTKPFIDPVTGNFTLTTDTVYAG
                     EVVNFLMSATDFDYHPLLATNDPSSGGFQRLVFVANGAEFGANYTNPNAGCIIPPCAT
                     LSPPPPQPPVLAATAVTFNWQTSCAHLGYTNGCVNFSNEYTFIIKSL"
     gene            1491..1871
                     /locus_tag="PLO59_00800"
     CDS             1491..1871
                     /locus_tag="PLO59_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99657.1"
                     /translation="MFYPKQKMGMCNIDSKGVFSRKIAFAIGVIVVFFLTIRYLVYGG
                     YPLLAALALASTFTVINWYQFRNVFCVAKALNLKPKNSKQQPQTNTDKINAHKYVAVT
                     IILRSILMAGIFSLVIWLIGLLVK"
     gene            2000..2425
                     /locus_tag="PLO59_00805"
     CDS             2000..2425
                     /locus_tag="PLO59_00805"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQU99658.1"
                     /translation="MSIVPVPDFSIPVEKQAQNPKSMQKLLLVEDDRVNQMLSRKMLL
                     KAGFEVDVANDGLEAKKLLACNLYHLVITDIKTPNMDGYALTKHIRDTECLRNLPVIA
                     VSAYPSSIEKQKALEAGMNEYIAKPYGMHELVETIKLFL"
     gene            complement(2478..>4477)
                     /locus_tag="PLO59_00810"
     CDS             complement(2478..>4477)
                     /locus_tag="PLO59_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HQU99659.1"
                     /translation="GTKVADPYRWLEYDTAANVKAWVDTQNRITFDYLAQIPYREKIK
                     KRLSEVYNYARYSSPFRAGDYYFFSKNDGLQNQSVIYFQKGLEGKPEVFLDPNTMSSD
                     GTSAVSLLGKSKDNKYMAYAINKAGSDWQEIYIMEIATKKLLADKLEWVKFSGAAWFG
                     NGFYYSRYDKPEAGKELSNKNEFHKVYYHTLGQLQSDDKLIYENKKFPLRYFWGQTTE
                     DEKFLMVYESEGTDGVAIHVKDLSKPGSTFDEVLKGFDHNASVINNVNNQLLIQTDAA
                     APNQRLVLVDPKNSAMDKWQEVLPEKPELLSSVTTAGGKIFAEYLKDVTTRVYQYDMD
                     GKNQKQINLPDLGSANVFGGNADDAVVFYSFTSFMYPSTIYKYVIANNKSEVWQKSDV
                     KFNAADYETKQVFFNSKDGTKVPMFIVHKKGIKLDGNNPTLLYGYGGFNASLSPTFST
                     SRLVLLENGGIFCLANLRGGGEYGETWHEAGMLLKKQNVFDDFISAAQYLIDNKYTSS
                     EKLAIQGGSNGGLLVGACMTQRPELFKVCFPAVGVMDMLRYHKFTIGWGWAVEYGSSD
                     SLVHFKNLYSYSPLHNLKPAKYPATLVTTADHDDRVVPAHSFKFAATLQEMNKGDNPT
                     LIRIDVKAGHGAGKPTSKVIEEQADIWSFMFKNMQITPIYK"
BASE COUNT         1390 a          843 c          848 g         1396 t
ORIGIN      
        1 ccagcgattt aattataaaa gtatattcat tactaaagtt tacacaacca ttggtataac
       61 ctaagtgggc acaactggtt tgccaattaa aagttaccgc tgttgcagct aaaaccggtg
      121 gttgcggtgg tggtgggctt aatgtagcac atggtggaat aatacaacct gcattggggt
      181 tggtataatt tgcaccaaac tcggcaccat tggctacaaa caccaatcgc tgaaatccac
      241 ctgaagacgg atcgtttgtg gctaagagcg gatgatagtc aaaatcggta gcactcatta
      301 aaaagttaac cacctcacct gcataaacgg tatcggttgt tagtgtaaaa tttccggtaa
      361 cagggtcaat aaatggtttg gttacagtag gcggtaaatt aggtacaccg gggctaagtg
      421 agttacaaac ttttagtgtt atctgtaagt cgcgaaatat ttcggcaatc acaataccac
      481 atttataagc cgatacttta ttaactgtaa caaatacgcc atttgtaaat gaagtgtaac
      541 taacctcacc ggttgttgag tttaaggtag cggcaacatt gtttggattt tgtgccgggc
      601 cgggtaatgg tgaagtaaaa gagtacccgg ggctaaagcc aataggtgcc ggatttgaag
      661 ctgtccatgc acctcctgta aaatcatcta aaggctgagc ccaactataa accaaactgt
      721 caatttcagg gtcaatagca gtaggattat aggtaaaagg atagccacta caaatgatgg
      781 tgtttggcga ttcaacaaaa aacgggcttg agtcgaaaca agggtttgca ttttgaaggt
      841 tgaacgagta cataatagcc cttaatgtaa aacccaatcc acccgcattg ggcaaattgg
      901 tgattgccga gttgcgacaa cagtttgaat aactaaaaat ccaacctgca gcaggtggca
      961 caccgtttaa aattatgggg ttcgattgaa aataaaactc ttcaactgcg ccagctgtac
     1021 taccggttgc acctgcacag gtgataggta cgcctgagtt tgcattacag tcgggtgata
     1081 tatcggtttg tgatataata gtcatcggaa tacttgtaag accgggctta tttgatacgt
     1141 caagactaat accagttgta ggaaaagaaa caccgttaca atcgcgataa agcttcataa
     1201 aaaaacgata ctcgccagtt ggcaaacact gccaggtaat ttcgccaccc attaaatgtg
     1261 atgcgctact ttgcaaaaaa caagatgtaa gtaataatag aaataatagt cttttcattt
     1321 gaatcaaatc agggtttatt ttgtgggtaa gcaaatgtaa aaaataaaat ggaggctctt
     1381 tttaaagtat gccattttac atttgtgtgc attgtcattg tatgagtcta ttaattttaa
     1441 aaagttgcat gtttacatgg ttctttatta agattaatca acttaaaaaa atgttttatc
     1501 cgaaacaaaa aatgggcatg tgcaatatag atagcaaagg tgtatttagc cgaaaaattg
     1561 cttttgccat aggcgtaatc gtagtgtttt ttttaaccat tcgctacctt gtttatgggg
     1621 gatacccgtt gttggctgct ttggcacttg caagtacatt tacggtaata aattggtatc
     1681 aatttcgaaa tgtgttttgt gttgcaaagg cattaaactt aaaacccaaa aacagcaaac
     1741 agcaacctca aacaaatact gataaaataa acgcgcataa atatgttgcg gtaaccatta
     1801 ttttaaggtc aatattaatg gcgggtatat tttcattagt aatttggctt atcggtttat
     1861 tggtgaaata gcaattttaa agtactgcat taaaattttg cccataaaat tgttgtgtta
     1921 ataaacaata ctattacttt agccaaatat ttatatgggt attaatattc gatgtttcta
     1981 atctaatcat ttaagagtta tgtcaatagt gccggtacct gatttttcaa ttccggttga
     2041 aaagcaggct cagaatccaa aatcaatgca aaaactatta ttggtagaag acgatagagt
     2101 aaatcagatg ctaagtcgaa agatgctttt aaaagcaggc tttgaagtgg atgttgccaa
     2161 tgatggttta gaggcaaaaa agttgttggc ttgcaacctg tatcacttgg taattaccga
     2221 tataaaaacg ccaaatatgg atggttatgc acttaccaaa catatacgcg atacagaatg
     2281 cttacgcaac ttgccggtaa ttgctgtatc ggcataccca tcatctatcg aaaaacaaaa
     2341 agctttagag gccggtatga atgagtatat agctaagcct tatggcatgc atgagttggt
     2401 agaaaccata aaactttttc tataaaatta caaacaaaaa aggcagcctt tttagctgcc
     2461 ttttttgttt ggataaccta cttgtatata ggtgttattt gcatgttttt aaacataaat
     2521 gaccaaatgt cggcttgttc ttcaataacc ttactggtgg gttttccggc accatgacct
     2581 gccttcacat caatacgaat taaggtaggg ttatctcctt tattcatttc ctgaagtgta
     2641 gctgcaaatt taaacgaatg tgcaggcacc actctgtcgt catggtcggc agtggtaacc
     2701 aaggttgcag gatacttagc gggctttaag ttatgtaatg gagaatagct gtataaattt
     2761 ttaaagtgaa ctaagctatc actcgatcca tattcaacag cccagcccca gccaatggta
     2821 aacttatggt agcgtaacat atccattacg cctacagccg gaaaacaaac tttaaaaagt
     2881 tcggggcgtt gcgtcataca agcacctaca agtagcccac cattagagcc accttgtata
     2941 gctaactttt cggaactggt gtacttatta tctatcagat attgcgctgc acttataaaa
     3001 tcatcaaata cattttgctt tttcagcaac ataccggctt catgccacgt ttcgccatac
     3061 tcgccaccac cacgcaggtt tgccaaacaa aatatacctc cgttttcaag caaaaccaaa
     3121 cgcgaagtac tgaacgttgg cgataatgat gcattaaaac cgccataacc atacagtaaa
     3181 gttggattgt ttccatcaag ctttatacct tttttgtgca caataaacat gggtactttg
     3241 gtaccatctt tactattaaa aaatacttgt ttggtttcat aatcagcagc attaaatttt
     3301 acatccgatt tttgccaaac ttctgatttg ttgttggcta taacatactt gtagattgta
     3361 gaaggataca taaatgatgt aaaactgtaa aacactacag catcatcggc attgccgcca
     3421 aaaacattag ccgaacccaa atccggtaag tttatttgtt tttggttttt accatccata
     3481 tcatattggt aaacccttgt ggtaacatct tttaaatatt cggcaaaaat tttaccacct
     3541 gcagtagtta ctgacgataa caactccggt ttttcgggca acacttcctg ccacttatcc
     3601 attgcactat ttttaggatc taccaacacc aatctttgat taggtgcggc tgcatctgtt
     3661 tgaatgagta actgattgtt tacattattt ataactgatg cattgtggtc aaatcctttt
     3721 aatacttcgt caaacgtact gccgggctta cttaaatctt ttacatgtat ggcaacacca
     3781 tcagtacctt cgctttcata caccattaaa aatttttcat cttcagttgt ttgtccccag
     3841 aaataacgta agggaaactt tttattttca taaatcaatt tatcgtcact ttgtaattga
     3901 cctaaggtat ggtagtaaac tttatgaaat tcgtttttgt ttgaaagctc tttgcctgct
     3961 tcgggcttat catatcgcga gtaataaaaa ccattgccaa accaggcggc accactaaac
     4021 ttaacccact ctaatttatc ggctaataat ttttttgttg caatttccat tatgtaaatt
     4081 tcttgccaat cgcttccagc tttatttatg gcataagcca tgtatttatt atcctttgat
     4141 ttgcctaaga gcgatacagc cgaagtgcca tcgctgctca tggtgttggg atctaaaaaa
     4201 acctcgggtt tgccttctaa tcctttctga aaataaatta cactttgatt ttgtaaacca
     4261 tcatttttgc tgaaaaaata gtagtctccg gctctgaacg gtgatgagta tcgtgcataa
     4321 ttataaactt cacttaaccg ctttttaatt ttttcgcgat agggtatttg cgccaaataa
     4381 tcgaacgtaa ttctgttttg agtatcaacc caagctttaa cattggctgc tgtatcgtat
     4441 tcgagccagc ggtatgggtc ggcaactttg gttccga
//
LOCUS       DAOMAA010000028         4445 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_12771_length_4445_cov_2.843508, whole genome shotgun sequence.
ACCESSION   DAOMAA010000028 DAOMAA010000000
VERSION     DAOMAA010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4445
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12771_length_4445_cov_2.843508"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="PLO59_00815"
     CDS             complement(<1..374)
                     /locus_tag="PLO59_00815"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQU99660.1"
                     /translation="MMRLKSSINFNFVLLGLALCSCASINYRNGNASYEEYAYADAIK
                     KFERVFEKSKYKDAKYKLAECYRLTGNNEKALALYARLVRMSESPNQIKYYYAQALMR
                     AGDYKNAKTWFDYIDGKRRRCA"
     gene            complement(393..1313)
                     /locus_tag="PLO59_00820"
     CDS             complement(393..1313)
                     /locus_tag="PLO59_00820"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HQU99661.1"
                     /translation="MKQFIFLLILFLACTQIQAQQAPVYSQYMFNPLIINPAYAGSKP
                     YMSATLLTRKQWLNFKGSPFTSSATIHTPLKNKRMGLGLSVSNDQVGITNQTDLYGSY
                     AYKLPIKEGNLSFGISGGVSFYKSNLSNLTVWDANDVVYEVNVLTNQLPNFGAGLFYS
                     TDKWYAGFSVPQLLSYSWRNGIHDGTNAVHRPVQHYFLTGGYAFEKSEDLVLKPSVLF
                     RYVQNAELQFDLNLNVLINKIIWVGASYRYKDAMVAIFEYQLSRKLRLGYAYDYPLSK
                     INSISVGSHEIMVGFDFGYDIMKVRNPRYF"
     gene            complement(1325..2302)
                     /locus_tag="PLO59_00825"
     CDS             complement(1325..2302)
                     /locus_tag="PLO59_00825"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HQU99662.1"
                     /translation="MPYHTTCFGTADGTITANVLGGASPYSYLWSTSQSTQTISNNPA
                     GTYTVLVTDAAGCTNTNSFVLTSPPQIIASVAGNQLLCGMDSTTIVATQPIQGVGYWN
                     VVSGFGTIDNVNASNTYITSIPLGTTILNWIVSDGFCSDTAIMSITVSESITAIAGVD
                     RIVCDNVINLAASDPQFGYGYWNVISGAAILFDSLQENTEAFNLSFGANVFQWKVVNG
                     NCSDSNLVTITLQTPENCLDPIEIPSGITPNSDGKNDVFFIRNLQDYPINNLEIFNRW
                     GMKVFEANSYQNNWSGQSNQGDLPDGTYFYILNVKSINKTYRGYIDLRH"
     gene            complement(2354..>4445)
                     /locus_tag="PLO59_00830"
     CDS             complement(2354..>4445)
                     /locus_tag="PLO59_00830"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HQU99663.1"
                     /translation="NYSVSVIDVKGCTASNNVSLVTTPKPVLSVDSIGAVSCNGFAQG
                     SIAISVSSGTPTFNYLWSNGATTEDIGSLLAGLFTVVVTDNAGCLVSQVFTVSEPTIL
                     NASLFIQNPSCGNTNGALTANVTGGTPGYTYLWSNGFTTQTISSLSIGSYTVTVTDSL
                     GCMQNASAALVDFGLPVITLSGINNVLCNGASNSSIDIAVSSGGGFYNYLWSNGATTE
                     DLSNIPAGTYTVTVTDITGCSASDTYITIEPTAIQVFLNKQNATCGVANGSANVTAQG
                     GVGAFTYLWSNGNTTDSIVFLNPGIYTITVTDANNCTASASALINNINAPVITVNSVK
                     AVSCAGLSDGKIDVNITGGVFPYFYLWNNGATTQDLFNIPSGVYTLTLTDANNCVTIY
                     THTINEPDTLTATITSTFASCNLSDGSASVIVQGGTLPYSYIWGAGSQSQTQPGVAAG
                     IYTVTVTDDNGCVISQSVAVTNPASPQIVGALVTDVNCFGLNNGAIAYNIFNGTAPFN
                     FVWSNGETTQNIASLISSTYTVTVTDSANCITSANFIVSQATQIQAVTNSINANCGFA
                     NGVAQVVSVSGGFSPYQFLWSDGTTSTAITNVFAGSYSITVTDSVGCAITSIVNVNNT
                     DGPQITLLDSTNVTCYGGNNGTIAIEVNGGVAPYQVVWSGTNQTTLNIDSLTAGQFTA
                     TVSDAFGCLAFYTV"
BASE COUNT         1415 a          881 c          778 g         1371 t
ORIGIN      
        1 tcggcgcatc gacgtctctt tccgtcaata tagtcgaacc atgtttttgc atttttataa
       61 tcacctgcac gcattaacgc ttgtgcatag tagtatttaa tttgattggg actttcggac
      121 atacgaacta aacgtgcata taatgccaaa gccttttcat tatttccggt taagcgatag
      181 cactctgcca gtttatattt tgcatccttg tatttacttt tctcaaaaac acgctcaaac
      241 ttttttatcg catcggcata agcatactct tcataagatg cattgccgtt tcggtaatta
      301 atacttgcgc aactgcataa ggctaaacct aaaagaacaa agttaaaatt tatactactc
      361 tttaatctca tcatttacct gtaataacgt atttaaaaat aacgcggatt tcgaactttc
      421 ataatatcat atccaaaatc aaaacctacc attatttcat gtgaacctac tgaaattgaa
      481 tttatttttg ataatggata atcgtaagca tagcctaagc gtaatttgcg actaagctga
      541 tattcgaaaa ttgcaaccat agcatcttta tagcgatacg aagctccaac ccaaattatt
      601 ttgtttatta atacatttag atttaagtca aattgcaatt cggcattttg tacataacga
      661 aacaagaccg atggcttaag cactaagtct tcactttttt caaatgcata accacctgtt
      721 aaaaaataat gctgtacagg tctgtgtact gcattggtgc catcgtgtat gccattacgc
      781 caactatagc ttagcagttg aggcaccgaa aagcctgcat accatttatc ggtactataa
      841 aataaaccgg ctccaaaatt gggaagttgg ttggtaagca catttacttc atatactaca
      901 tcattagcat cccaaacagt aaggttgctt aaatttgatt tataaaagga aacaccacct
      961 gaaataccaa atgataagtt gccttcttta ataggcaatt tataagcata acttccatat
     1021 aaatcggttt gattggttat acctacttga tcattactta cacttaaacc taaacccatg
     1081 cgtttgtttt taagcggtgt atgtatggtt gctgatgatg taaatggcga acctttaaaa
     1141 ttgagccact gcttgcgtgt aagcaaagtt gcactcatat agggtttcga gcctgcatac
     1201 gcaggattaa taataagcgg gttaaacatg tattgactgt aaaccggtgc ctgttgcgct
     1261 tgtatttgtg tacatgccaa aaataaaatt aaaagaaaaa taaattgttt cattttatgc
     1321 atgattaatg gcgaagatca atatatcctc tataggtttt atttattgat tttacattta
     1381 aaatataaaa ataggttcca tcaggcaaat cgccttgatt actttgacct gaccaattgt
     1441 tttgatagct gttggcttca aaaactttca tcccccatcg gttaaaaatc tccagattgt
     1501 tgattgggta atcttgcaaa ttccttataa agaaaacatc attttttcca tcactattag
     1561 gtgtaattcc acttggaatt tcaatgggat ctaaacagtt ttcaggtgtt tgtagcgtga
     1621 tggttaccaa attcgaatcg ctgcaattgc cattaactac cttccattga aacacattgg
     1681 caccaaagct taggttaaat gcctctgtgt tttcttgcaa tgaatcaaat aaaattgcgg
     1741 cacctgaaat tacattccaa tacccatatc caaattgtgg atcggaagct gctaagttaa
     1801 tcacattatc acaaacaatc ctgtcaacac cagctatagc tgttatgctt tctgaaactg
     1861 taattgacat tattgctgta tcagaacaaa aaccatcact cactatccaa tttaaaatgg
     1921 tggtgcctaa tggtattgat gtgatgtaag tattgctggc atttacattg tcaatggtgc
     1981 caaatcccga aaccacattc caataaccaa caccttgtat aggttgagtt gcaacaattg
     2041 tggttgaatc cataccacat aatagttgat tacctgcaac gcttgcaatt atttgcggag
     2101 gacttgtaag cacaaaagag ttggtgttgg tgcatcctgc tgcatcagta actaatactg
     2161 tataggtacc agccggatta ttacttatgg tttgggttga ttgactggtt gaccataaat
     2221 aactataagg cgatgcgcca cctaaaacat ttgctgttat agttccatcg gctgtgccaa
     2281 agcaggttgt atgatatggc acatttagct gcggtatggt tgcagtgata agaagtgaat
     2341 caccttcgct taattatacg gtataaaaag ctaaacaacc aaaggcatca gaaacggtag
     2401 cggtaaattg gccggcagtt aaactatcaa tgtttaatgt ggtttgattg gtacctgacc
     2461 atactacctg gtaaggtgca acacctccat ttacttcaat agcaatagta ccattattgc
     2521 caccatagca agttacattt gtcgaatcta aaagtgtaat ttgtgggccg tctgtattat
     2581 taacgtttac aattgatgtt attgcacatc caacagaatc ggtaacagtt attgaataac
     2641 ttccggcaaa cacattggta attgctgtac ttgtggtacc gtctgaccat aaaaactgat
     2701 aaggtgaaaa accgcctgaa acagacacta cctgtgccac accatttgca aaaccacaat
     2761 ttgcatttat actattggtt acggcttgaa tttgtgttgc ctgacttaca atgaaattag
     2821 cacttgttat acaattggca ctatctgtaa ctgttaccgt ataggtgctt gaaatcaatg
     2881 atgcaatatt ttgtgttgtt tcgccattag accatacaaa attaaacggt gcagtgccat
     2941 taaaaatgtt gtaagcaatt gctccgttat ttaatccgaa acaattaacg tcagttacta
     3001 aggcacccac tatttgaggc gatgccggat ttgttactgc cacactttgg ctaattacac
     3061 agccgttgtc atcagtaaca gttactgtat atataccggc agcaacaccc ggttgcgttt
     3121 gactttggct acccgcaccc caaatatatg aatatggtaa agtaccgcct tgaacaataa
     3181 ccgaagcact gccatcactc aaattacaag atgcaaaggt tgaagtaatg gttgctgtta
     3241 atgtatcagg ctcgttgata gtatgtgtat aaatggtaac acaattatta gcatcagtaa
     3301 gtgtaagtgt gtacacacca gatgggatat taaatagatc ttgcgtagtt gcaccattat
     3361 tccacaaata aaagtagggg aatacgccac cagtaatatt cacatcaatt ttaccatcac
     3421 ttaagccagc acatgaaaca gcctttaccg aattaactgt aattaccgga gcattaatat
     3481 tgttaattaa tgcacttgca gatgctgtac aattattggc atcggtaacg gtgatagtat
     3541 aaattccggg attcaagaat acaatcgagt ctgtagtatt tccattagac cacaaatagg
     3601 taaatgcacc aacgccacct tgcgctgtaa cattagcaga accatttgcc acaccacagg
     3661 ttgcattttg tttatttaaa aatacctgaa tggctgttgg ttcaattgta atatatgtat
     3721 ccgaagccga acatccggta atatctgtta cagttactgt ataggtgcca gccggtatat
     3781 ttgaaaggtc ttcggtagtt gccccattac tccataaata attataaaag ccaccaccgc
     3841 tagatacagc gatatcaata ctgctgttgc tggcaccatt acataaaaca ttatttatac
     3901 ctgataatgt gataaccggc aatccaaaat caaccaatgc tgccgatgca ttttgcatac
     3961 aacctaacga atcggttaca gttacggtgt agctacctat tgataatgat gaaatagtct
     4021 gagttgtaaa tccgttcgac cataaatagg tatatccagg tgttccacca gttacatttg
     4081 ctgttaacgc tccgtttgta ttgccgcaag atggattctg aataaacaaa cttgcattta
     4141 atatggttgg ctcgctaacg gtaaacacct gcgataccaa acaacctgca ttatcagtaa
     4201 ccacaactgt aaataaaccg gctaaaagcg aaccaatatc ctcagttgtt gcaccattac
     4261 tccacagata attaaaagta ggtgttcccg atgaaaccga aatagcaata gagccttgtg
     4321 caaaaccatt gcaacttacc gcgccaatac tatcaacact taatacaggc ttaggtgttg
     4381 taactaaact aacattgttt gaggctgtac aaccttttac gtcaataaca gaaactgaat
     4441 aatta
//
LOCUS       DAOMAA010000029         4364 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_13195_length_4364_cov_3.100951, whole genome shotgun sequence.
ACCESSION   DAOMAA010000029 DAOMAA010000000
VERSION     DAOMAA010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13195_length_4364_cov_3.100951"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1359
                     /locus_tag="PLO59_00835"
     CDS             <1..1359
                     /locus_tag="PLO59_00835"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU99664.1"
                     /translation="DCDVFNSVAGNTMTNCATGLLLNSAARIGTQLHKGNKWINTQGG
                     STKAENLNTVNNNFLNSEIVYNPNSGTVYAPTIIIPPQWFSPDVTGITYNGCATQVCN
                     TAIAGVGSGDEEQLKNVARDSAISYDYLAENRNQAQQNLYAVLYQDSLLRVSDSVFAN
                     FFGNHQSEAIGQLHLVGKNMAFETVYDSLFKHTINQADSLIGIKADSLLLTTNASQRQ
                     WLINGIRLLQDSIQALLSQKQVAAVSMNEIASSINEAIVVNEIPENNLKAINGMKLLC
                     NKYDTDTLLHFYSTIESIAQQCPYSGGKAVFQARVLLCLFNDSIMYNDATVCLQSGIY
                     RNLMQQDETATLSFFDFSLLPNPASQQVQVAFNFTIEKPMQFEIRNVLGGKVSEFEID
                     KGLKSIVFTTQNYEDGIYFIRMRSNGLSLMTKKLIIIKNETHGFSFCLAVDNGNGFCS
                     GI"
     gene            1292..2719
                     /locus_tag="PLO59_00840"
     CDS             1292..2719
                     /locus_tag="PLO59_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99665.1"
                     /translation="MKRTVLVFASLLITVMGFAQGYNHNFLIGDDAVPFPPYTGYAKG
                     IIKTDSLNFGIFGLSRKMPFVSAQSNIANDTGAILFYTNGCWIANVLDDTMQNGAALN
                     PDPYVTQWCAPPFALPYPQANIILPWPNDTAKYVLFHHGGSQADPNSQPLISYYSIID
                     MTLDSGLGGVIQGQKNVVLIADSLNVGFTACKHANGRDWWVVILKGNSNLIYTVLLTD
                     QGVQQPFTQSIGVPDVDRGTKQPVFSADGLKFAYRFATSGMGNVINQVRVFNFDRCNG
                     TFSNAQIVSWTSNYPSPGCSFSSNSKYLYTVSDFDTIYQINTDTSNIQASMQVVAVND
                     GYFSPIPPFQSSFWTTYLAANGKIIISSGSGVVDLHYINYPDSAGVACDVQQHALPMP
                     CFVFRCHVNHPNYYLGRLQGSPCDTLQWAGISEAEHDFRFRMYPNPVTNNSLHIGYLL
                     PHNSAGSSYSGLFQIYDVTGKVVFK"
     gene            3090..3833
                     /locus_tag="PLO59_00845"
     CDS             3090..3833
                     /locus_tag="PLO59_00845"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HQU99666.1"
                     /translation="MSKLKTPIIMAYEFTDAEVIKVIDENDVVKRFWIKMPEASAFTF
                     KAGQFIMHDLPIDSKYTNRSYSIASAPTTDGIFELCIVLNPKGLGTPFIFENFKPGYT
                     CKVSRNLGKFQVPEPIETDLCFICTGTGIAPLRSQVFDIINRNLPHKNMYMVFGNRWE
                     KDILYRQEMEELAQTHPQFKFIPVLSRTNDNWQGAQGYVHAIYEEIFADKRPAKFFIC
                     GWADMLKEARQRLEAMGYDKKAIKFESYD"
     gene            complement(3961..>4364)
                     /locus_tag="PLO59_00850"
     CDS             complement(3961..>4364)
                     /locus_tag="PLO59_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99667.1"
                     /translation="DALGNKKESLEMYEKLYSQTKSLKHGYQMAVLQFGLQRYGECNT
                     TCDALLTNNKVTTEKISINVDQSNTQEVPLSAAIHNLKGVMYKEMNQNDKAKGEFEIA
                     LKEDATFVLATKNLEAISTKAESPKEDKKKK"
BASE COUNT         1329 a          808 c          845 g         1382 t
ORIGIN      
        1 gactgcgatg tgtttaacag tgtagcgggc aataccatga ccaattgcgc cacaggatta
       61 ttgctaaaca gcgctgcccg cataggcacc caattgcaca aaggcaacaa atggattaat
      121 acgcaaggcg gcagtactaa ggcagagaat ttgaatactg taaataataa ttttttaaat
      181 tcagaaattg tttacaatcc aaattcaggc actgtatatg ccccaacaat tattattccg
      241 cctcaatggt ttagccctga tgtaacaggt ataacctata acggctgcgc aacacaggtg
      301 tgcaatactg ccattgcagg agtaggaagt ggcgatgaag agcaattaaa aaatgtggca
      361 cgtgatagcg ccatatctta tgattacttg gcagaaaatc gcaatcaggc acagcaaaat
      421 ttgtatgccg tgttgtatca ggattcctta ttgcgtgtaa gcgactctgt ttttgctaac
      481 ttttttggga atcatcaatc cgaagctatt ggacaattgc atctagttgg taaaaatatg
      541 gcattcgaaa ccgtttatga ttcactcttt aaacatacca tcaaccaggc cgacagtttg
      601 attggtatta aagcagatag tttgctatta acaaccaatg cttcacaacg acaatggctg
      661 ataaatggca ttcgtttatt gcaggattca attcaagctt tgttaagtca aaagcaagta
      721 gctgctgtat caatgaatga aattgcttca tcaatcaatg aagcaatagt tgtcaatgag
      781 ataccggaaa ataacctaaa agctattaat ggcatgaaat tgctttgcaa caaatatgat
      841 acagacaccc tgcttcattt ttatagtacc attgaaagca tagcgcaaca atgtccttat
      901 agcggaggca aagctgtgtt tcaggcaaga gttttattat gcttgtttaa tgacagcata
      961 atgtataacg atgccaccgt atgtcttcaa agtggcatat acaggaattt gatgcaacag
     1021 gatgaaaccg ccacgctatc gttttttgat tttagcttgt tacccaaccc ggcatcgcag
     1081 caagtgcaag tagcgtttaa ttttactatc gaaaagccga tgcaatttga aatacgcaat
     1141 gtattgggtg gcaaggtaag tgaatttgaa attgacaaag gcttgaaatc cattgttttt
     1201 acaacacaaa attatgagga cggcatttat tttattcgta tgcgcagtaa tggcttatcg
     1261 ttgatgacta aaaaactaat cattataaaa aatgaaacgc acggttttag tttttgcctc
     1321 gctgttgata acggtaatgg gttttgctca gggatataat cataattttt taattggtga
     1381 tgatgcagtt ccgtttccac cttatacggg ttacgctaaa ggaataatca aaactgactc
     1441 tttgaatttt gggatatttg gcctttctag aaaaatgcct ttcgtaagtg cacaatctaa
     1501 tattgctaat gatacgggtg caatactttt ttatacaaac ggctgttgga tagcaaatgt
     1561 attggacgat acaatgcaaa atggtgctgc tttaaatccc gacccttatg taacacagtg
     1621 gtgtgcacct ccattcgcat tgccctatcc ccaagcaaat attattcttc catggccaaa
     1681 tgataccgca aaatatgtgc tttttcatca tggcggtagt caggctgacc caaattcgca
     1741 accattaatt tcttattatt caattattga tatgacttta gatagcggat taggcggagt
     1801 tattcaagga caaaagaatg ttgttttaat tgcagatagt ttaaacgtgg gttttactgc
     1861 ctgcaaacat gccaacggca gagattggtg ggttgttatt ttaaaaggca acagcaattt
     1921 aatttatact gttttgctta cggatcaagg agttcaacaa ccatttacac agtcgattgg
     1981 cgtgcctgat gttgatcgtg gtacaaagca accggtattt tcggccgatg gattaaaatt
     2041 tgcttatcgt tttgcaacaa gcggaatggg taatgtaata aatcaagtgc gggtttttaa
     2101 ttttgacagg tgcaacggca ctttttcaaa tgcacaaatt gtatcatgga caagtaatta
     2161 tcccagtccg ggatgttcct tttcttccaa ctccaaatat ctttatacgg tttcggattt
     2221 tgatactatt tatcaaatca atacagatac ttccaatata caagcaagta tgcaggtagt
     2281 ggctgttaac gatgggtatt tttcgccaat cccgcctttc cagtctagtt tttggacgac
     2341 ctacttagcg gccaatggaa aaattataat tagttcaggt agcggtgtgg ttgatttgca
     2401 ttatatcaat tatcctgata gtgcaggcgt tgcttgcgat gtgcagcagc atgccttacc
     2461 aatgccctgc tttgtatttc gttgtcatgt caatcacccc aactattact taggtcgcct
     2521 gcaaggctcg ccatgtgata cgttgcagtg ggcaggtatt tcagaggcag aacatgattt
     2581 ccgttttcgc atgtatccaa accctgttac aaataattct ttacacatcg gatatttatt
     2641 accacataat tcggcaggct catcatacag tggtttattt cagatttatg atgtaacagg
     2701 taaggttgtt tttaaataaa ctttgccgca gtggagcaat gagcaaagtt taactttacc
     2761 caaattaagc aatggaattt atcaatgcat tttaagatca gacaatcagc ttgcaacaat
     2821 gaaattgatt gtactaaaag agtaatcatg tcaatccttt aatcataaga atcatggttc
     2881 agacaatcgc ccatgatggc taaaaatgca agtggtatct gcatataaaa ttatacacgc
     2941 gctaatcttg atattttgct aaaccccatt agcgaattac tttaaaataa attgatgcgc
     3001 gctaacgcac aggtggttag ctattgaagt aaaaacaatt tttataactt aatttagaaa
     3061 attaacaacc tgtttaaaaa gttttatgtt tgtcgaaact caaaacccca ataataatgg
     3121 cttacgaatt taccgatgct gaagtaatta aagtaattga tgagaatgat gttgttaaac
     3181 gcttttggat taaaatgccc gaagcttcgg cttttacgtt taaagcaggt cagtttatca
     3241 tgcacgattt acccattgac tcaaaataca ccaacagaag ctactccata gcatcggcac
     3301 caactaccga tggtatattt gaattgtgta tcgttttaaa ccctaaagga ttaggcaccc
     3361 cttttatttt cgaaaatttt aaaccgggct atacctgcaa ggtgtcgcgc aacttaggta
     3421 agtttcaggt gcccgagcca attgaaaccg atttatgttt tatatgtacc ggcacgggta
     3481 tagccccatt gaggtcgcag gtatttgata ttataaaccg aaatttgcca cataaaaaca
     3541 tgtacatggt ttttggtaac cgctgggaaa aagatattct ttaccggcaa gaaatggagg
     3601 aacttgcaca aacacatcca cagtttaaat ttataccggt gctaagccgt accaatgata
     3661 actggcaagg ggcacaggga tatgtgcatg ccatatatga agaaattttt gccgataaac
     3721 gtcctgcaaa attttttatt tgtggctggg ctgatatgtt gaaagaagcc cgccaaaggt
     3781 tagaagcaat gggctatgat aaaaaggcga ttaagttcga atcgtatgat taggataaat
     3841 ttatttttcg ctttttaatt ttttttgaaa cgctataaaa gccaaaaact ttttaaatta
     3901 tttgatacaa aaaaaacggc tgatgaaaac accagccgtt tttttatgat tcatagcgaa
     3961 ttactttttc ttcttatctt ctttagggct ttcggctttc gtacttattg cctccaggtt
     4021 tttggtagcc aacacaaatg ttgcatcctc ttttaaagct atttcaaatt cgcctttggc
     4081 tttatcgttc tgattcatct ccttatacat aacacctttt aagttgtgta ttgctgctga
     4141 tagcggtacc tcctgtgtat tcgactgatc aacgttgata cttattttct ctgtagttac
     4201 cttattgttg gttagcaatg catcacaggt ggtattacac tcaccataac gttgtaagcc
     4261 aaactggagc acagccattt ggtaaccatg ctttaaactt ttggtttggc tataaagctt
     4321 ctcatacatt tcgagcgatt cttttttatt gcccaatgca tctt
//
LOCUS       DAOMAA010000030         4338 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_13354_length_4338_cov_2.357693, whole genome shotgun sequence.
ACCESSION   DAOMAA010000030 DAOMAA010000000
VERSION     DAOMAA010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4338
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13354_length_4338_cov_2.357693"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>4338)
                     /locus_tag="PLO59_00855"
     CDS             complement(<1..>4338)
                     /locus_tag="PLO59_00855"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99668.1"
                     /translation="GGPNVNLGLASASPTATSGAGTTNFGVVFDAYSTFTLNTVKIYP
                     VSATNASGTVTIDVINSAGAIIHSATVPVTGSPAASLTPAIVNLNFTIAPGTNYKLRP
                     GSFTGITGLLFMPSGGPSNYGYPFVYPGVVSLNTSTLTAAPTNTARNDLYYYFFDWNI
                     STGCESSRTPVVATITPPPALSVTPNLNACYNTYTALTITSPNGNYDSYTWSPVTNMY
                     LDTLGTPYTANTNADTVYVLSTSVANIVYTCTGSNSAPPFCADTASVAVAFQQNASVA
                     TTATPNPSCAGQPVVLTASLGAPATVVLGTNNNATKIGGSNGNLYRTGTTLNLEMRTQ
                     YLITAAELSAAGLQAGAMNSVGFYVLTSSTPGVLSNFTLRLGHTTATALTATYQIGPF
                     TDVWFAPTFTPVIGLNTHTFTTPFMWNGVDNVVIESCNVLTTGGSGTTLQTFTTPVQT
                     NMANLVAGGCSAAAGGSTGFTNCRPVIEINGSTAPTATAYSWSDGSNVVGTTSPLTVN
                     PTISTGYICTITANGCPYVSDTVNQIVNTLPTAPTASAPSTQCGYGVPTVSVTSTTGA
                     PTPIFNWYSALTGGTLLQTGTSNTYTNAIGATTTFYVVEVNTNGCESNPRVAATANVS
                     AYDSIYITSDTLTCEGTPVTFNVVKVGSLNSYTYTWTATPLPGSGVTGSPTGASQVFT
                     PTTQGNYVYSVTGVDGLCTANATKIVTIYPGLSGSLSATQITSCNNLNGSITANVIGA
                     GTVFSSDFTSPVIPGNMTYAGNDFTINGGVMQLTSSAAAKNGGVVITNTTGLANNDFQ
                     IDFDLITSPGSNPAADGFSYSYGPDVVELPTGTGSATVGTVVVPNTVNPENGSGTALK
                     LAFDAYSNGANVEGIYLMYNTPVWNQTPASTGVIAHSTDVSWRATNLIGKTTHVTIKI
                     NGSGQVSLWLNNILIVNNQPLPAAYLSANKASWKHAFSSRTGALYQGTAIDNLTIQYN
                     NFYEYSLNAGPWSTTNPIPASGVGVYTVDARYVAVPACSTNLGSVTINTPVFTTTTSA
                     SSVCAYGPVMPLLSVVPSFAGATYQWQISPANANAFVDISGATSATYTPLVSEITANT
                     DFRCVILCSGTPIPGSPSVPVTITYDQPQVLTTLGGSRCGIGSVALNATASSGAGLSW
                     YANATGGAPLGTGTTFNTPNISVTDTFYVAAGAGSSSENVASPNIGTSTFITATTGWG
                     LRFTANSAATISSVTVRALSSSPGAASIQIKVSDLNDVVLYTGTLHNFTITNALADYV
                     IPVNINVAPGNYKMGMTFTNITSMVRESGGVTFPYNAPSSAISITAGANGSAGAQTTS
                     AYYWFYNWVISTGCSSTRTAVIATVTSAPTVTVSPNVIQCEGSSTVLTASSINDPNYT
                     YTWNPGGFVGSTYLVTPAVTTTYTLTAVDSSGGANNGCQDIQTVTVTVTPNPVINSIT
                     ATPGFVGQG"
BASE COUNT         1238 a          894 c          950 g         1256 t
ORIGIN      
        1 tcgccttgcc caacaaagcc gggcgtggca gtaatactgt taatcacagg attaggagtt
       61 actgtaaccg ttacagtttg tatatcctga catccgttat tagcgccacc cgaactatca
      121 actgcagtta atgtataagt tgtagttact gcaggtgtaa ccaaataggt tgatcctaca
      181 aaaccaccgg gattccatgt atatgtgtaa tttgggtcat ttatgctgga tgctgttaac
      241 acggtactcg agccttcgca ttgtattaca ttgggcgaaa ctgtaactgt tggtgccgat
      301 gtaactgttg ctataacggc tgtacgtgtg ctagagcaac cggtgctaat aacccaatta
      361 taaaaccaat aataagcaga tgttgtttgc gcaccggctg atccattagc gccagccgta
      421 atcgaaatgg ccgaagatgg ggcattgtat gggaatgtaa caccgcctga ctcacgaacc
      481 atagaagtga tattggtgaa cgtcataccc attttataat tgccgggtgc aacgtttata
      541 ttaacaggta tcacataatc ggctaaggca ttggttatag taaaattgtg caaggtacct
      601 gtatataaaa ccacatcatt caaatcgctc actttaattt gaatgcttgc agctccggga
      661 gaagagctta aagctcttac ggtaactgaa ctaatagtag cggctgagtt tgcggtaaat
      721 cttaaacccc aacccgtggt agctgtaata aaagtagaag tgccaatgtt tggcgaggcc
      781 acattttctg acgaagaacc tgctccggca gcaacataaa atgtatccgt tacgctgatg
      841 ttaggcgtat taaatgtggt gccggtgcct aacggagcac cgccagttgc attggcatac
      901 caactaagac ctgcacctga agatgcagta gcatttagtg ccacacttcc gataccacaa
      961 cggctaccac ccaaagtagt taatacctga ggctgatcat aagtaatggt tactggtact
     1021 gatggcgagc ccggtatggg tgtaccgcta cacaatatca cacatctgaa atcggtatta
     1081 gcagttattt cgcttactaa cggtgtgtat gttgccgagg ttgcacctga aatatcaaca
     1141 aatgcattag cgtttgccgg tgaaatttgc cattgatatg ttgcgcctgc aaaagagggt
     1201 acaactgata ataatggcat aaccggtccg tatgcacaaa cacttgaagc cgatgtagtt
     1261 gttgtaaata caggtgtatt tattgtaaca ctacctaagt tagttgaaca agccggtacg
     1321 gcaacataac gtgcatctac tgtataaaca ccaacaccgc ttgccggaat cggattggtg
     1381 gttgaccaag gtccggcatt taagctatac tcataaaaat tattgtattg aatagttaaa
     1441 ttatcaatag cagtaccttg atataatgca cccgtacgtg atgagaatgc atgtttccag
     1501 cttgctttgt ttgccgataa ataagctgca ggcaaaggtt ggttattaac tatcaaaata
     1561 ttgtttagcc acaatgaaac ctgacctgaa ccatttattt taatggttac atgcgtggtt
     1621 tttcctataa ggttggttgc gcgccacgat acatcggtac tgtgtgcaat aacgccagtg
     1681 cttgccggtg tttggttcca aacaggtgta ttgtacatta aataaatacc ctccacattg
     1741 gctccgtttg aataagcatc aaaagcaagt tttaaagctg taccacttcc gttttcaggg
     1801 tttacggtat ttggtacaac cacggttcca acagtagcac tacctgtacc agttggtaac
     1861 tcaaccacat cagggccata gctgtaacta aatccatcgg ctgccggatt acttcccgga
     1921 gaggttatta aatcaaaatc aatttgaaaa tcattgtttg ccaagcctgt agtatttgta
     1981 attactacgc caccattttt agcagcagcg ctactggtta attgcattac accgccatta
     2041 attgtaaagt cgttaccggc ataagtcata ttacctggta ttaccggact tgtaaagtcg
     2101 ctgctaaata cggtacctgc accaattaca tttgcagtaa tactgccatt taaattgtta
     2161 caagatgtaa tttgtgtagc cgacaaactg cccgataagc ccggataaat agttacaatt
     2221 ttggtagcat tggccgtaca taagccatca acaccggtta ctgaataaac ataattacct
     2281 tgtgttgtgg gtgtaaatac ctgacttgcc cctgttggtg aacccgttac tccactgcct
     2341 ggcaacggtg tagccgtcca tgtataggta taactgttta acgagcctac tttaacaaca
     2401 ttaaatgtaa caggagttcc ttcacaggtt aaagtatcgg atgtaatata aatgctgtca
     2461 taagcagaaa cattagctgt tgctgcaaca cgtgggttgc tttcgcaacc atttgtattt
     2521 acttcaacaa cataaaatgt ggttgtagca ccaattgcat ttgtataagt attgctggta
     2581 ccggtttgta gtaaagtacc tccggtgagt gcactatacc aattaaaaat aggcgtaggc
     2641 gcaccggtag tagatgtaac tgaaaccgtt ggcacgccat atccacattg tgtagatggt
     2701 gccgatgccg taggtgctgt tggtaatgta tttactatct ggttaaccgt atcgctaaca
     2761 taaggacaac catttgctgt aatagtacaa atatagcctg tgctgatagt tggattaacg
     2821 gttagtggac tggtggtacc aaccacatta cttccatcac tccaagaata tgctgtagca
     2881 gttggtgctg tgcttccgtt tatttcaatt accggacggc aattggtaaa tccggttgag
     2941 cctccggctg ctgcactaca accaccggct actaaatttg ccatattggt ttgcaccggt
     3001 gtagtaaagg tttgcagtgt agttccactt ccgcctgtag ttaacacatt acatgattca
     3061 attaccacat tatctactcc gttccacata aatggtgttg taaaggtgtg tgtatttaat
     3121 ccaattaccg gtgtaaatgt tggtgcaaac caaacatcgg taaatggtcc gatttgataa
     3181 gttgccgtta atgcagtagc tgtagtatga cctaacctta aagtaaagtt acttaatacc
     3241 cctggtgtac tgctggttaa tacataaaaa ccaacactat tcattgcacc ggcttgtaaa
     3301 ccggctgccg ataattcggc agcagtaatc aaatattgcg tacgcatttc taagtttaaa
     3361 gtagtacccg tacggtataa gtttccgtta gagcctccaa tttttgttgc attgttgttg
     3421 gttcctaaca caacggttgc cggtgcacct aaagatgccg ttagcacaac cggctggcct
     3481 gcacacgatg gattaggagt tgccgtggtt gcaactgaag cgttttgttg aaatgcaact
     3541 gcaacacttg ctgtatcagc acagaaaggt ggtgctgagt tactaccggt acatgtgtaa
     3601 acaatatttg ccactgatgt tgataaaaca taaaccgtat cagcatttgt attggctgta
     3661 taaggcgtac ctaaagtatc caaatacata ttggtaaccg gtgaccatgt gtatgagtca
     3721 taattcccat taggtgaggt aattgtaagt gcagtataag tattataaca tgcatttaaa
     3781 ttaggcgtta ccgataatgc tggaggtggt gtaatggttg caacaaccgg tgttcgtgag
     3841 ctttcgcaac cggtactaat gttccaatcg aagaaatagt aatataagtc gttacgagcg
     3901 gtattggtgg gtgcagctgt tagcgtactg gtgttaagcg ataccacacc cgggtaaaca
     3961 aaaggatatc cataattgct aggtccgcct gatggcataa acaataatcc ggtaatgccc
     4021 gtaaaactac ccggccttaa tttatagttc gtaccgggcg ctatggtaaa gtttaggttt
     4081 acgattgccg gtgttaatga tgcagccggg ctgcctgtta caggtactgt tgccgaatga
     4141 attatggctc ctgcactatt tataacatca attgttaccg tacctgatgc attagtggca
     4201 cttaccggat atatttttac ggtatttaaa gtaaaggttg aatatgcatc aaacacaact
     4261 ccaaaattgg tggtacctgc tcctgatgtt gcagtaggag aggccgatgc caaacctaaa
     4321 tttacattag ggccaccg
//
LOCUS       DAOMAA010000031         4325 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_13425_length_4325_cov_2.876112, whole genome shotgun sequence.
ACCESSION   DAOMAA010000031 DAOMAA010000000
VERSION     DAOMAA010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4325
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13425_length_4325_cov_2.876112"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="PLO59_00860"
     CDS             <1..256
                     /locus_tag="PLO59_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99669.1"
                     /translation="GALYFNQGAELANRANQLPLSKQKEVDALNKQAAEKLKMAEPYL
                     EKALQINPTDESTLQSLKQLYVRTGSTEKYEAIKKQLEKK"
     gene            complement(531..1298)
                     /locus_tag="PLO59_00865"
     CDS             complement(531..1298)
                     /locus_tag="PLO59_00865"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HQU99670.1"
                     /translation="MTLFTQNLKVVLFCVLSITLIFQNEAVIAQSSEQVWLEYMLNIP
                     IKTKYNIEFASTYATEIETPKWHSLDFQITPEVALNKHVDFMAAILGSHTYQAKSVTT
                     KEIREMIGVRLHIAPDKRVKTRLLTRLEHRNLYSDESEIWETNMRLRIRAETITPLVL
                     DKVYTSNNQCYMIADLEQFISLNHEVHERFANRLRARLGIGYLIHYGLRLELVYTLQL
                     SKNNFEDGYNSTENIFRFRLKQYLNKNKPSKAQGTGN"
     gene            complement(1435..1881)
                     /locus_tag="PLO59_00870"
     CDS             complement(1435..1881)
                     /locus_tag="PLO59_00870"
                     /inference="COORDINATES: protein motif:HMM:NF015317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqhA family protein"
                     /protein_id="HQU99671.1"
                     /translation="MNQVFKVFVFIIAIVIFINFIIITLVGVYKTFHAVTLFFQSGLQ
                     HKPGIEIVESLDVFLVALLFLILSLSIMKLFYPENALFSSINLPWLQINDFVQLKNLT
                     WNAFLLTLLITFGTSVIKNSDHLDWTFLIIPIAVLLFAISAKMLRH"
     gene            complement(1890..3572)
                     /locus_tag="PLO59_00875"
     CDS             complement(1890..3572)
                     /locus_tag="PLO59_00875"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HQU99672.1"
                     /translation="MKKVMFLKYLTIFVCLTTIACQQKVSKEKPVKQPNILLIVADDM
                     GFSDIEPFGGNISTPILTQLSKESSLFSNFHVLPTCSPSRSVMLTGNDNHVAGLGIMG
                     EADYPVLQNQNLPGYAGYLSNQVATIPELLRNNGYHTYMTGKWHLGEGPGRDPYDRGF
                     EQTFILSTGGGSHWSDKKALAPPQHMSYTRNGKEVEPPQDFYSSRNYTDSMIQFIKIN
                     KADEKPFFAFLSYTAVHDPLHAPKEYIEKYKGKFDMGWDSLYMQRLKNLQALGIVPQS
                     LNRFAKNPTVPQWNKLDQHHKEEYARDMEVYAAMLDYMDMSIGRVFEYLKKEGMYDNT
                     MIIFTSDNGANGQMASFYPGNEDGKYLGSFENEMENRGSKNSYIEMGPGWAQAVSAPF
                     KFFKTFTTEGGIKSPLVIKMPGKNAGGKWNNSLLHVSDLMPTILELTNTAYPLEFNGN
                     KIHALIGNSIMPILTEQAGEVTRTNGIGYELFEMKAFIKGKWKILRLPKPMGTGNWQL
                     FDLNTDPGETTDLSEQYPEIKAELINGWNEYAKTNEVYNHNGHFDSIYQRSF"
     gene            complement(3576..4244)
                     /locus_tag="PLO59_00880"
     CDS             complement(3576..4244)
                     /locus_tag="PLO59_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99673.1"
                     /translation="MKLIILCFGMAISFVNTMAQTDREQLSLAVSNAEAQNQQKLKEY
                     VWKRKSQVFIESQLKLTTVTEFSYTPEGKLNTKIIDATTTVKKKPGIRGAAQENAAES
                     KLDYIEKALALAMSYTFMSKGEMIDFFSKATITKSAEKIIAIASDVHVSGDKLEIHID
                     PKSNLYLFKKFESLLGNEKVAGKLNYENFQNGTNHISTTTLNLPAQKMDIEAQNQDYT
                     IRIK"
BASE COUNT         1393 a          766 c          702 g         1464 t
ORIGIN      
        1 aggtgcctta tactttaacc aaggtgccga gttagcaaat cgtgcaaatc aattaccgtt
       61 aagtaagcaa aaagaagtag atgcattaaa caaacaagct gctgagaaat taaaaatggc
      121 tgagccatac ctggaaaaag cgcttcaaat taatccaact gacgaaagta cattacaatc
      181 attaaaacaa ctttacgtaa gaacgggctc aaccgaaaaa tacgaagcca ttaaaaagca
      241 attagaaaag aaataatttg cgttattaaa tcaacttagt tttaacataa agagacggta
      301 aaaatactac cgtctttttt ttatgcatta gttaatttca aatttcacaa atgccattta
      361 taaattacgc tttcagcaaa ttgaataata aagaaaggga atgaattaac tcaacgtata
      421 tagcaatagt gcaattatca tacttaacat aatattaatt atggtaattt agtataaagc
      481 ttttcaatag gtttctacta ttaaaaaatt ctgattacag gctaatattt ttaattaccg
      541 gtaccttgag cctttgatgg tttgttttta ttcaggtatt gctttaaacg aaaccgaaat
      601 atattttctg ttgaattgta tccatcttca aaattatttt tagacaattg taaggtgtaa
      661 accaattcta aacgcaaacc gtaatgtatt agatacccga tccctagcct tgcgcgcaac
      721 cggtttgcaa aacgttcatg aacttcatgg tttaatgaaa taaattgctc taagtctgca
      781 atcatataac attgattatt agaagtataa accttgtcta aaacaagtgg tgtaattgtt
      841 tcggccctta ttcttagccg catattggtt tcccaaattt cactttcatc gctatagagg
      901 tttcgatgtt ctaaacgcgt taacaaccgg gtttttacac gcttatcagg tgctatatgc
      961 agccgcaccc caatcatttc ccgaatttct tttgttgtta cagatttagc ttgataggta
     1021 tgactaccta aaatagctgc cataaaatca acatgcttat ttagagcaac ctcaggcgtt
     1081 atttgaaaat ctaaactatg ccatttgggt gtttctattt cagtagcata ggtgcttgca
     1141 aactcaatat tatactttgt tttaattgga atattgagca tatactcaag ccaaacctgt
     1201 tcgctgcttt gcgcaataac agcttcattt tgaaaaatta atgtaatact taaaacacaa
     1261 aagaggacaa cttttaagtt ttgcgtaaac aaagtcataa cgataacaaa agttagaaag
     1321 acctaaaata atttggtgtt taatagcata ctaaaagttg taaattttaa gagttaagca
     1381 attttgttta taaatattga attagcaaaa caaatgctcg tttcttccta aaaactaatg
     1441 ccttaacatt ttagcactaa tggcaaaaag caaaacagca attggaatga taaggaaggt
     1501 ccaatccaaa tgatcgctgt ttttaataac cgatgtacca aaggttatca gtaatgtcaa
     1561 aagaaatgca ttccaggtta agtttttcaa ttgtacaaag tcattaatct gcaaccaggg
     1621 taaatttata gaactgaata atgcattttc aggataaaac aatttcataa tactaagtga
     1681 taggattaaa aaaagtaatg ccactaaaaa aacatctaaa gactcaacta tttcaatacc
     1741 gggcttatgt tgtaggccgc tttgaaaaaa taatgtaact gcatgaaaag ttttataaac
     1801 acctactaat gtgataatga taaagtttat aaaaattacg attgcaataa tgaaaacgaa
     1861 tactttaaaa acttgattca tgactataac taaaagcttc gttggtaaat cgaatcaaaa
     1921 tgtccattat gattatatac ttcattcgtt ttggcatatt cattccaacc gtttattagt
     1981 tcagctttta tttcaggata ttgttcggat aaatctgtgg tttcgcccgg gtcggtattt
     2041 aaatcaaaca actgccagtt acctgtgccc attggtttgg gtagtcttaa tattttccat
     2101 ttacctttaa taaaagcctt catttcaaaa agttcatagc caataccatt tgtgcgggtt
     2161 acttcaccag cttgctccgt taatattggc ataatagaat taccgattag cgcatgaatt
     2221 ttattaccat taaattcaag tggataagct gtatttgtta gttctaaaat ggtaggcatt
     2281 aaatctgata catgcaagag gctgttattc catttcccgc cagcattttt gccaggcatt
     2341 tttataacaa gtggagattt tataccgcct tcagttgtaa atgttttaaa aaatttaaac
     2401 ggtgccgaca cagcctgggc ccatcccggt cccatttcaa tatatgaatt tttagagccg
     2461 cgattttcca tttcattttc aaatgaacct aagtatttac cgtcttcatt tccagggtag
     2521 aaacttgcca tctgaccatt tgcaccatta tctgaagtaa aaatgatcat cgtattatca
     2581 tacatcccct cttttttcag atattcaaat acacgaccga tgctcatatc catgtagtcc
     2641 agcatagcgg cataaacttc catatctcgg gcatattcct ccttgtgatg ttgatcgagt
     2701 ttgttccatt gtggtactgt aggatttttt gcaaaacgat ttaaactctg cggaacaatt
     2761 ccaagcgctt gtaaattttt aagccgttgc atgtaaaggg aatcccaacc catatcaaat
     2821 ttacctttgt acttttcaat atattctttt ggagcatgca aagggtcatg cacggctgta
     2881 taagaaagaa atgcgaagaa tggtttttca tccgctttat ttatcttaat gaactggatc
     2941 attgaatccg tgtaatttct tgaagaataa aaatcctgag gtggctctac ttctttacca
     3001 tttctagtat atgacatgtg ttgtggtgga gccagtgctt ttttatcgct ccagtgactt
     3061 cctccacctg tgctaagtat gaaagtctgc tcaaatccgc gatcataagg atctcgacca
     3121 gggccctcac ctaaatgcca cttaccagtc atataggtat gataaccatt attgcgtaac
     3181 aattcgggta tggtggcaac ttggttgcta aggtaaccag cgtatcccgg aagattttga
     3241 ttttgcaaaa cagggtaatc agcctcgccc ataatgccca accccgcaac gtgattatcg
     3301 ttacctgtta gcattaccga acggcttggt gagcaagttg gcagcacatg aaaatttgaa
     3361 aacaggctac tctcttttga taattgtgtt aaaatgggcg tgcttatgtt gccaccaaaa
     3421 ggttcaatat cactaaaacc catatcgtca gcaactatga gcaatatatt aggttgctta
     3481 acaggttttt ctttcgaaac tttttgttga catgcaatcg ttgttaagca aacaaatatg
     3541 gtgaggtatt ttaaaaacat aacttttttc aattattatt taatgcgtat ggtataatct
     3601 tgattttgtg cttcaatatc cattttttgt gcaggcaggt ttaacgttgt tgttgaaatg
     3661 tgattagtgc cgttctgaaa attttcataa ttaagtttac cggcaacttt ttcattcccc
     3721 aacaaacttt caaatttttt gaataggtaa agatttgatt tagggtcgat atgaatttct
     3781 aatttatcac cgctaacatg cacatcactt gcaatagcaa taattttttc tgctgatttt
     3841 gttatggtag ctttactgaa gaaatcaatc atttctcctt tcgacataaa ggtataactc
     3901 atagccaaag ccaatgcctt ttctatataa tctaatttac tttcagctgc attttcttgc
     3961 gctgcgcctc ttatgcccgg cttctttttt acagttgttg tggcgtcaat tattttggta
     4021 ttcaatttac cttcaggtgt ataactaaat tcagtaacgg tagttaattt taattggctt
     4081 tcaataaata cctgcgactt ccgtttccaa acatattctt ttagtttttg ttgattttga
     4141 gcttcggcat tagatacagc cagtgataat tgttcgcgat ctgtttgagc cattgtgttt
     4201 acaaaactaa tggccatgcc aaaacataaa ataataagtt tcatttttaa tttattaagt
     4261 aataaaattg tattggtaat attaatataa cctaaaatgt attgccacat ttatattcaa
     4321 aatgt
//
LOCUS       DAOMAA010000032         4299 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_13570_length_4299_cov_3.168944, whole genome shotgun sequence.
ACCESSION   DAOMAA010000032 DAOMAA010000000
VERSION     DAOMAA010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4299
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13570_length_4299_cov_3.168944"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..750
                     /locus_tag="PLO59_00885"
     CDS             55..750
                     /locus_tag="PLO59_00885"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HQU99674.1"
                     /translation="MKHNRRLFLKKGVAVAGALYLKPWQLNAQTISEYTLAPLPYAFS
                     DLEPHIDTKTMELHYGKHHQAYVTQLNKALANTGKPAPSLDNLMQTISKYGVAVRNNG
                     GGHFNHTLFWQMMQPNGGGQPQGKLADMITLQFGSFEKFKQAFKTAALSQFGSGWAWL
                     IQQRDTLKITTTPNQDNPRMDVVVKSEQGNPLLCIDVWEHAYYLKHQNMRANYIDAFF
                     NVVNWNFISKRLS"
     gene            973..2253
                     /locus_tag="PLO59_00890"
     CDS             973..2253
                     /locus_tag="PLO59_00890"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU99675.1"
                     /translation="MSFKITSIALLFCAASAEAQLGVLDSVFLGTGYTNEVYYNTQTM
                     TKTIVPKNNWELGFQNNLMQASIIANHTYGVTVYRCPNVSLSDFLNADTTGLTTWTPL
                     FNSDVTWNDGALNATRSANPFDFGWGTYDMGTHFVTGDSVFIIKLVTGPPGPGAVTEY
                     RKLAVTLKTPGGDYYVRQSNLDNTNDITDTIFKADYLYKNFGYYSLRNNSKLDREPIS
                     TDWDILFTRYYENVQPVGYYPVAGILSNIGVQVAEASGVDVLTVNAANYTSQFSTKIS
                     EIGSDWKFYDQPNNLWVIQDSLTYFVKSQTNLISQITLTGFGGSATGMVSFFVTSIPT
                     SVNEIQQANLVSMALQPNVCNQNTTLIFSSKLNSTYLCTITGLSGQIISQLSFNADAG
                     LNQKQLDVSELKAGIYFVTLTTNTEKLTAKLIKN"
     gene            2411..>4299
                     /locus_tag="PLO59_00895"
     CDS             2411..>4299
                     /locus_tag="PLO59_00895"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQU99676.1"
                     /translation="MGYHLAYGQKNYTVTVTSDTEPLAFATISLTTATGVSKQLQTGF
                     TNDLGQFEITVLPPLKLSVYKFGYATQTISVSQALPLQINLKPLGITLPNVTVSTAQV
                     NERPQENSVYKVKVITAKEIEMQSATNLRDILLTQLNIQVAADPVLGSSMALQGMGGQ
                     QVKILRDGVPVVGRENGSIDLSQMQLQNVARIEIIQGPASTVYGADALGGVINLISKP
                     IAHAGWQASVNSKYESVGTYNIDAATAWQKNNTGINVSGGRNFFNGYSASDTMRFQTW
                     KPREQLFGSIGATQKYKGASLVFKSDYLYETIYDFGNPTITPYQAYAFDYNYITKRFT
                     NTLQANKNWNKNSKSTASVSYQNYERIKKDYYKNLVTLENTQMITPPQDNKFNNVNIR
                     ADYSAWRNKWNYQIGTEINADYGTGAKIDGQKTMQDYALYNSFEWMPVSQLQIRPALR
                     FAQNSIYGFKAIPAINFSYKPCQPATIRASVAQGYRAPSLKELYLNFVDINHNILGNE
                     TLKPENSTNLQGSAEYTSFLKTLMFKISASGFYNHVQNRIMLSLIDQATNFYTYQNSG
                     KFISYGTGLETTLQSNVVKLDAGFMLTRIKFDNAGSDAKFLNTPEWRLACTYHYTKWN
                     MSTGL"
BASE COUNT         1430 a          847 c          780 g         1242 t
ORIGIN      
        1 atggccaaac tgtagaaaaa aatcaactac tgattacctt aatgtaacac taacatgaaa
       61 cacaaccgca gattgttttt aaaaaaagga gttgctgttg ccggagcact ttatttgaag
      121 ccctggcaat taaatgcgca aacaattagc gaatatacat tggcaccatt accctatgcc
      181 tttagcgatt tagaacctca tattgataca aaaaccatgg agttgcatta tggcaagcat
      241 caccaagcct acgtaacgca attaaacaaa gcattagcca ataccggtaa gccggcccca
      301 tcattagata acttaatgca aaccatttcg aaatatggtg ttgcagttcg aaataatggt
      361 ggcggccatt tcaatcatac tttgttttgg cagatgatgc aacccaatgg tggcgggcaa
      421 ccacagggta aattagctga tatgataacc ttacaatttg gtagtttcga aaaatttaaa
      481 caagcattta aaacagcggc attatcgcag tttggcagcg gctgggcatg gcttattcaa
      541 caaagagata cattaaaaat aactacaaca cccaatcaag ataatccacg tatggatgta
      601 gttgtgaaaa gcgagcaggg taatccatta ttatgtatag atgtttggga gcatgcctat
      661 tacctcaaac atcaaaacat gagagcaaat tacattgatg cattttttaa tgtggttaac
      721 tggaatttta tatcaaaacg gcttagttaa ttagctgacc aatttattaa gcttttatat
      781 agtaaggcta tttttctaaa tcattttaga tgcgtgtgta cacacctctg tttagttgca
      841 tggtgtaata catacactgt cagtactctg taaaattggg aatcaatgct ttttgtatga
      901 caagttagcg cagcacttaa atacctttga cgcctcattt tacatcttta aattcttatt
      961 ataattctta atatgtcttt taaaattaca tctattgcat tattattttg tgcagcaagt
     1021 gcagaagcac aacttggtgt tttagactca gtatttcttg gtacgggtta caccaacgaa
     1081 gtgtattaca acacacaaac catgaccaaa accattgttc caaaaaacaa ttgggaactt
     1141 ggtttccaaa acaacttaat gcaggcaagt attattgcca atcatactta tggtgttact
     1201 gtatatcgtt gtccgaatgt ttcgcttagc gattttttaa atgctgacac aaccggttta
     1261 actacatgga cgcccctgtt taattcagat gttacctgga atgacggtgc tttaaatgcc
     1321 acacgctcgg ccaatccgtt tgattttggt tggggcactt acgatatggg cacacacttc
     1381 gtaactggag actcggtctt tatcattaaa ctggttacag gccctcccgg cccgggtgca
     1441 gtaacagaat atcgcaaact tgctgtaaca ttaaaaacac ccggtggcga ttattatgta
     1501 cgtcaatcga acttagacaa caccaacgat attactgata ccatttttaa agctgattat
     1561 ttatataaaa atttcggcta ctactcatta cgtaataact caaagttaga tcgtgagccc
     1621 atctcaaccg attgggatat tttatttaca cgttattatg aaaatgtaca accggttggc
     1681 tattatcctg ttgcaggtat tttaagcaat attggtgtac aagtagccga agcaagtggt
     1741 gtagatgtgt taaccgttaa tgcagcaaat tatacatcgc aattctctac taaaataagt
     1801 gaaattggct ctgactggaa gttttacgac cagccaaata atttatgggt aattcaagat
     1861 tcgctaacct attttgtaaa atcacaaacc aacttaatat cacaaattac cttaacaggg
     1921 tttggaggtt cggctaccgg tatggtatcg ttttttgtaa catcaattcc tacatcggtt
     1981 aacgaaatcc aacaagccaa tttagtaagt atggcgcttc agccaaatgt gtgtaatcaa
     2041 aatactacat tgatttttag cagcaagtta aacagtacct acctatgcac tataacaggc
     2101 ctaagtggcc agataataag ccaattaagc tttaatgcag atgcaggttt aaaccaaaaa
     2161 caacttgatg taagcgaatt aaaagcaggt atttattttg ttacacttac aacaaatacc
     2221 gaaaaactta cggctaagct tattaaaaat taacgccctt atttgcagcg caccccagaa
     2281 gcggtagccg ttttacttaa caatttgatt ttttatcgtg ttaccctatc agcataaaac
     2341 taacaactat ccggcaaaac cgagtagtcg tttttattta ttgttaatcc tggttaccgg
     2401 cctattaaac atgggctacc atcttgcata cggtcaaaaa aactatacag taactgttac
     2461 ttccgatact gagccattag cgtttgcaac tatcagctta actacagcaa ccggtgttag
     2521 caagcaatta caaacaggtt ttaccaatga cctgggccaa tttgaaatta cggtattgcc
     2581 tccgcttaaa ttatcggttt acaagtttgg ttatgcaaca caaaccatta gtgtatcgca
     2641 ggccttgcca ttgcaaataa atttaaaacc attaggcatt accttaccca atgttaccgt
     2701 aagcacagca caagtaaacg aacgcccgca agaaaattcg gtttataaag taaaagtaat
     2761 tactgccaaa gaaattgaaa tgcaaagcgc caccaacctg cgcgatatat tgcttacgca
     2821 attaaacata caagtggctg ccgacccggt tttaggaagt agcatggcac tacaaggcat
     2881 gggcggccag caagtgaaaa ttttacgcga tggtgtaccg gtagtaggtc gcgaaaatgg
     2941 cagtatagat ttatcgcaaa tgcaattaca aaacgttgcc cgtatcgaaa taattcaagg
     3001 gcctgcatca accgtttatg gggccgatgc attaggcggt gttataaatt taattagtaa
     3061 accaattgca catgcaggct ggcaagcatc agtaaacagc aaatatgaaa gtgttggcac
     3121 ttacaatatt gatgcagcaa ctgcatggca aaagaataac acaggcatta atgtaagcgg
     3181 tggtcgtaat ttttttaatg gttattcggc atctgatacc atgcgttttc agacctggaa
     3241 accacgcgaa caactttttg gaagcattgg cgccacacaa aaatataaag gtgcttcgtt
     3301 agtatttaaa tcagattatt tgtacgaaac catttacgat tttggtaacc ctaccattac
     3361 accttatcag gcctatgcct ttgattataa ttatataacc aagcgcttta caaatacttt
     3421 acaagcaaat aaaaactgga ataaaaattc gaaaagtaca gcatctgtat cttatcaaaa
     3481 ttatgagcgt attaaaaaag attattataa aaatttggta acgcttgaaa acacgcaaat
     3541 gattacgcca ccgcaggata ataaatttaa caacgttaat atccgtgctg attatagtgc
     3601 atggcgcaat aagtggaact atcaaatagg tacagaaata aatgccgact atggtaccgg
     3661 tgctaaaatt gacggacaaa aaaccatgca ggattacgcc ctatacaata gttttgaatg
     3721 gatgcctgta agccaactgc aaattagacc tgctttgcgt tttgcacaaa actcgattta
     3781 tggatttaaa gccataccgg caattaattt cagctataaa ccctgccagc ctgctaccat
     3841 cagagccagt gttgcgcaag gatacagagc accatcgcta aaagaattgt acttaaactt
     3901 tgttgatata aaccataaca tattgggcaa cgaaacactt aagcccgaaa actcaactaa
     3961 tttacaaggc agtgccgagt acacatcatt tttgaaaaca cttatgttca aaatttcggc
     4021 atcgggtttt tataaccatg tacaaaaccg cataatgctt tcattaattg atcaggcaac
     4081 taatttttat acctatcaaa acagcggtaa atttataagc tacggtaccg gtttagaaac
     4141 caccttacaa agcaatgtgg ttaagcttga tgcaggcttt atgttaaccc gaataaaatt
     4201 tgacaatgcc ggcagcgatg ccaaattttt aaacacgcct gaatggcgtc tggcttgcac
     4261 ctatcattat accaaatgga acatgagcac cggtttgtt
//
LOCUS       DAOMAA010000033         4284 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_13677_length_4284_cov_2.764247, whole genome shotgun sequence.
ACCESSION   DAOMAA010000033 DAOMAA010000000
VERSION     DAOMAA010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13677_length_4284_cov_2.764247"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="PLO59_00900"
     CDS             <1..269
                     /locus_tag="PLO59_00900"
                     /inference="COORDINATES: protein motif:HMM:NF019159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HQU99677.1"
                     /translation="SNSNAVYVVPPVTYKYYYNTIGAIKAFASGWKQLQHGADMELYL
                     SDHADWYDILNVISQTQPRYIITHHGDGSHLQSHFKNKIEVLVY"
     gene            376..3051
                     /locus_tag="PLO59_00905"
     CDS             376..3051
                     /locus_tag="PLO59_00905"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002999400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HQU99678.1"
                     /translation="MSNKLSTIETKYNPQTVENKWYAHWLQQGFFKSIPDQRTPYTIV
                     IPPPNVTGVLHMGHMLNNTIQDALIRLKRMQGFNACWVPGTDHASIATEAKVVALLKE
                     QGIEKNNLTRDEFLKHAWAWKEKYGGIILKQLEKLGASCDWDRTRFTMEDDLSEAVID
                     VFIDLHNKGHIYRGVRMVNWDPKGLTAVSDEEVIHKEVNSKLYYVSYPIADADDEITE
                     AWIKNKQQQGHTITIATVRPETILGDTAVCVHPDDERYKHLHGKFAVIPFVNRKVPII
                     LDDYITMDFGTGALKVTPAHDINDYNLGVKHNLEVIDTIAANGTMSDAAQFYIGMDRF
                     AVRKQIVADLQNAGHLLKTEDYKNKVGYSERTDAVIEPRLSMQWFLKMDTISKPALEA
                     VINGDVKLIPDKFINTYKHWMENVHDWCISRQLWWGQRIPAWYDKHGNFVVAKTENEA
                     KKQLATKLNLSVENIEITQDEDVLDTWFSSWLWPISVFDGFKDPDNKDINYYYPTNDL
                     ITAPEILFFWVARMLIAGYEYRGQKPFTNVYLTGIVRDKLGRKMSKSLGNSPDPIELI
                     EKYSADGVRVGMLLSSPAGNDLPFDEALCEQGRNFANKVWNAFRLTQGWTTDADLQPT
                     AGENIAMQWFENKFNKSLADINLHYDKYRMSDALMATYKLVWDDFCAWYLEMIKPAFG
                     APISQKVYDVTMHYFENILKVMHPWMPFITEELWHLITERNPADCIIVANWPVLAAVN
                     ESINNQFELFAETVTQIRNIRASKGISPKETLPLFIKQKPTENPFSPLIIKLGNLSQI
                     AFTDTTVAQATSFLVGEVEHFILLANKIDTAAEKEKLQAEINYLIGFLKSVDAKLNNN
                     NFTAKAKPEVLVMEQKKKADTEQKIQLLQQQLATF"
     gene            complement(3190..>4284)
                     /locus_tag="PLO59_00910"
     CDS             complement(3190..>4284)
                     /locus_tag="PLO59_00910"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HQU99679.1"
                     /translation="DGWEIINNTIHDITNIGIDASGGYKVSKNVALDVARNGVIRGNV
                     VYNCKSPYASAAGIYADGADDVLIERNICYNNQYGFEIGCEEKNSIASGIIMRENIAY
                     NNDEAGIALGGYNYPNTGKVDDCYINNNTFYNNDLTQKGQGELVLTHLENSVFINNIM
                     HGGNKRLLFNSAIGNLLGNTFDYNNWFTIDTDSTKPMFKINGSNYKTFADYVGSALGW
                     DSHSVFGNPNFVSGADFHLTTGSFAYNLGDPAFVAGLGETDFDLAPRKYDNRVDAGAY
                     ESQVPLRSTVSTYESKILTVYPNPCTTVLNISCDAEVQKIRVLNLSGNEVIVLPPIQI
                     LNIENFTSGFYILEVITSDQTILRKTFIKR"
BASE COUNT         1438 a          833 c          800 g         1213 t
ORIGIN      
        1 aatcaaacag caatgcagtt tatgttgtac ctccggttac atacaaatat tattacaata
       61 ccattggtgc tataaaggca tttgcatcgg gctggaaaca actgcaacac ggggccgata
      121 tggaactata cctcagcgac catgccgatt ggtatgacat attaaatgtt attagccaaa
      181 cacaacctcg gtatataatt acacatcatg gcgatggcag ccacctacaa tcgcatttta
      241 aaaacaaaat agaagtgctg gtttattaag gattaaaaac attaacaggc tgtattatat
      301 taaagccctt aactacaaac gctgcaacaa attcattaat tcaaaaagta tattcgcccc
      361 attaacgcct tttatatgtc aaacaagcta tcaactatcg aaaccaaata caatccgcaa
      421 accgtagaaa acaaatggta tgcacattgg ttgcaacaag gtttttttaa atcaataccc
      481 gaccaacgta caccctatac gatagttata ccgccaccca atgtaaccgg tgtactacac
      541 atgggccaca tgctaaacaa taccattcaa gatgcattaa tccgcttgaa acgcatgcaa
      601 ggctttaatg cctgctgggt acccggtacc gaccatgcat ctattgccac cgaagcaaaa
      661 gttgttgcac tgcttaaaga acagggcatt gaaaagaaca atttaacgcg tgatgaattt
      721 ttaaaacatg cctgggcctg gaaagaaaaa tatggtggca tcattttaaa acaattagaa
      781 aaattaggtg cttcatgcga ttgggaccgc accagattta caatggaaga cgaccttagc
      841 gaagccgtta ttgatgtgtt tattgatttg cataataaag gtcatattta tcgcggtgtg
      901 cgcatggtaa attgggatcc gaaaggacta actgccgtta gtgatgaaga agtaatacac
      961 aaagaagtta attcaaaact ttactatgta agttatccca ttgctgatgc tgatgatgaa
     1021 ataactgaag catggattaa aaacaaacaa caacaaggcc acactataac cattgccaca
     1081 gttaggcccg agactatttt aggcgatacc gctgtttgcg tgcatcctga tgacgaacga
     1141 tacaaacatt tacatggtaa atttgccgta ataccatttg taaaccgcaa agtgcctatc
     1201 atacttgacg attacattac catggatttt ggtaccggtg cattaaaagt tacacctgca
     1261 cacgatataa atgattacaa cttaggcgtt aagcacaatt tagaagtaat tgacaccatt
     1321 gcagctaatg gaaccatgag tgatgctgcg cagttttata ttggtatgga tcgttttgcg
     1381 gttcgtaaac aaattgttgc cgatttgcaa aatgcaggac acctgcttaa aaccgaagat
     1441 tataaaaaca aagtaggata cagtgagcgt accgatgccg ttattgaacc gcgtttaagc
     1501 atgcaatggt ttttaaaaat ggataccata agcaaacctg cattagaagc tgttattaac
     1561 ggtgatgtaa aactgatacc cgataaattt ataaatacct acaagcattg gatggaaaat
     1621 gtacacgact ggtgtattag ccgccaactg tggtgggggc aacgcatacc tgcctggtat
     1681 gacaagcatg gaaattttgt tgttgccaaa accgaaaatg aagcaaagaa acaacttgcg
     1741 actaaactaa atttatcagt cgaaaacata gaaattacac aagacgaaga tgttttagat
     1801 acctggttta gctcctggtt atggcccata agtgtatttg atggatttaa agatccggat
     1861 aacaaagaca ttaactacta ctacccgacc aacgatttaa ttacagcacc tgaaatttta
     1921 tttttttggg tagcacgtat gcttatagcc ggttatgagt atagaggcca aaagccattt
     1981 accaatgtat atctaacagg cattgtgcgc gataaattag gacgaaaaat gagtaagagc
     2041 ttaggcaact cgcccgaccc gattgaactt attgaaaaat acagtgccga tggtgtgcgt
     2101 gttggtatgt tgcttagttc gcctgcaggc aacgatttgc cctttgacga agccctgtgc
     2161 gaacaaggtc gtaattttgc taataaggtt tggaatgcat tccggctaac gcaaggatgg
     2221 actaccgatg cagacctgca acctactgct ggtgaaaata ttgctatgca atggttcgaa
     2281 aataaattta ataagtcgtt agccgatata aatctgcatt acgataagta ccggatgagc
     2341 gatgcattaa tggctacata taagttagtt tgggacgact tttgtgcctg gtatttagaa
     2401 atgattaaac cagcttttgg cgcaccaatt agccagaaag tatatgacgt aaccatgcat
     2461 tacttcgaaa acatattaaa agtaatgcac ccttggatgc catttatcac cgaagagttg
     2521 tggcatttaa ttacagaacg taatcctgcc gattgtataa ttgttgcaaa ctggcctgta
     2581 ctggctgctg ttaatgaatc tattaataac cagtttgaat tgtttgccga aacggttact
     2641 caaatcagaa acattcgggc atcgaaaggc atttcaccta aagaaacatt gcctctattt
     2701 attaaacaaa aaccaactga aaatccgttt agtcctttaa taataaaact gggtaacctg
     2761 agccaaatag cctttaccga tacaacagtg gcgcaggcaa catcgtttct ggtaggtgaa
     2821 gttgaacact ttattttatt agccaacaaa attgatacag cagccgaaaa agaaaaattg
     2881 caagcagaaa taaattacct gattggattt ttaaaatcgg tagatgctaa acttaacaac
     2941 aacaatttta cagctaaagc aaagcccgaa gtgcttgtaa tggaacagaa gaagaaagcc
     3001 gatacggagc aaaaaataca gttactacaa caacaattgg caacatttta atcggactta
     3061 ccgagaagtt gatacaagtt tttttatgaa taatatgtgg ccgtgattaa atggctgcta
     3121 ccaaaataaa acacgctatg caattgcata gcgtgttttt attaaagcct taattcaagc
     3181 attaggattt taccttttta taaaagtttt ccttaaaatg gtttgatctg aagttatgac
     3241 ttccaaaatg taaaatccgc ttgtaaaatt ctcgatattt aaaatttgta tgggcggtaa
     3301 aacaatcact tcattaccac tcaagtttaa aacacgaatt ttttgtactt cggcatcaca
     3361 actaatattt aaaactgttg tacagggatt gggataaaca gtaagtattt tgctttcata
     3421 cgtactaaca gttgagcgca aaggtacctg cgattcgtat gcgcctgcat caacgcggtt
     3481 atcatatttc cttggggcca aatcaaaatc ggtttcgcct aaaccggcaa caaatgcagg
     3541 gtcgcccagg ttatatgcaa acgagcctgt ggttaaatga aaatcagcac ctgaaacaaa
     3601 attaggattg ccaaaaaccg aatggctgtc ccaccccaat gccgaaccta catagtcagc
     3661 aaaagtttta tagttagaac cattaatttt aaacatgggc ttggtactgt ctgtgtcaat
     3721 ggtaaaccag ttattataat caaaagtatt gcccagtaag ttgccaatgg ccgagttaaa
     3781 taaaaggcgt ttgttgccac catgcattat gttattaata aatacactgt tttctaaatg
     3841 tgtaagtacc aattcgcctt ggcccttttg cgttaaatca ttattgtaaa aagtgttatt
     3901 gtttatgtaa caatcatcca ccttacctgt attcggataa ttataaccac ccaaagcaat
     3961 accggcttca tcattattat aggcaatgtt ttcgcgcata ataataccac ttgcaatact
     4021 gtttttttct tcgcaaccta tttcaaaacc atactgattg ttataacaaa tattgcgttc
     4081 aattaaaaca tcatctgcac catcggcata aatgccggca gctgatgcat aaggcgattt
     4141 gcagttgtaa accacattgc ccctgataac gccatttcgg gccacatcta atgctacatt
     4201 tttcgatact ttataaccac cacttgcatc aattccaata ttggttatat catgaatggt
     4261 gttattaata atttcccatc catc
//
LOCUS       DAOMAA010000034         4269 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_13757_length_4269_cov_3.049359, whole genome shotgun sequence.
ACCESSION   DAOMAA010000034 DAOMAA010000000
VERSION     DAOMAA010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13757_length_4269_cov_3.049359"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2002
                     /locus_tag="PLO59_00915"
     CDS             <1..2002
                     /locus_tag="PLO59_00915"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HQU99680.1"
                     /translation="AESDNVYLDESELNAIYRLEITQERIKTEYPNLTSGQVRRKYES
                     LLVVRDRFLVGAYTGLRVSDFSRIGEMNIDENYIQLRYNNNWGNLYKCLYPADLENLG
                     TNANAYKLTAQGRRVYELKTNTVQDNYTDLKNFVQALHAPATTNYQYNLEKQFNVNSF
                     LRTYAFDVITGNWDNYAGNTNNYYLYNDWQTGKINYLPYDVDNTYGIDWLGKDWGTRN
                     VYNWPVNSANKYLITKLMAYPDYANRFTFYLNQIVAGAGSFAFQQTFIDNIKTQIETY
                     VINDTYYTLDYGYTFGDFINSYTQAIGGHVDYGLKPYISTRNAQAVSQMQVNQVAPII
                     SEKRHYPFRPVAGDTVFIKALVEDELTVVNPVLNYRYGQTATWLTTNMFDDGLHNDDA
                     SGDNIFGVALNPVSTKDTLYYYITCTDANNLTGREPRLYSDTLIIETPWAVSINEFMA
                     DNANVIADNYGEYDDWIELYNAGTTIVNQQLFLTDDFLKPDKWRITTPIANGFQIFWA
                     DDDEQQGSNHASFKLNATADKIAITEYTGKDFRFIDSLSYGVQTTNVSMGCYPDGAKP
                     IVTLASVTPMFSNVVAATFYNEFKTIVHPNPVNNILYINASTPLSSLQVFDVTGRVLL
                     EQKIQQSLHLIQLDVTNLPQGNYFIRLVGANGLANVKFCKQL"
     gene            2015..2512
                     /locus_tag="PLO59_00920"
     CDS             2015..2512
                     /locus_tag="PLO59_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013597889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HQU99681.1"
                     /translation="MLPQYIQKINTAKSKKGDIKKLLAFLQSKRPGDLDTVTNRLHDE
                     AFDNIDCLQCANCCKTTGPLLISKDVSRLAQAVKLAPGNFMNTYLKTDEDGDVVFKQM
                     PCMFLNNNNYCKVYHARPNACRDYPHTQQNKIIQKLEITLHNSMICPAVADVVAGLKK
                     YYMNK"
     gene            complement(2513..3451)
                     /locus_tag="PLO59_00925"
     CDS             complement(2513..3451)
                     /locus_tag="PLO59_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99682.1"
                     /translation="MAQQSLFTSSFKLFLKTYLIPVACIVIAAGGILNYFFEQKIILG
                     SSSNGAYKVNRIIHRPANEISILGSSRAEGAFIPSILSSHAFNYGLSGAQDNVMLYFL
                     SEALKQQSTQPIIINFDPEGLNSSLGDLSNYIYNASYQPVRKLMGNAYEPWFAIPFFK
                     YFGSFELYTKYYLNSKLQLTKVTDCGGNFEKNVLTPQKFASLIAERKNTTTTFKNDTA
                     LERDLINLLITNKVTPLVFVLSPIHNSYFVSYKNQSGFKTFIDSITQIPLVHFINMHH
                     SYPDSMFINTTHLNYNGAVVFSEQLKDSLQARGLLK"
     gene            complement(3417..>4269)
                     /locus_tag="PLO59_00930"
     CDS             complement(3417..>4269)
                     /locus_tag="PLO59_00930"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HQU99683.1"
                     /translation="VEGLTLMLYGFFKKLVVADRLAVYVNQVYGDIEHASSISVALAC
                     IFFSFQIYCDFSGYTDIARGAARMMGYELMLNFNRPYLATSIANFWSRWHISLSTWFR
                     DYLYIPLGGNRCSKIKWYRNLSVVFLVSGLWHGANWTFVVWGALHACYTIFELVTKNT
                     RIRFWQSIGLYQTKIMHACSVLFVFIAVTFAWIFFRAENISQAWQVIHKLLAFDFTLN
                     MAQLTAEKGPLNLALCFLSIVLLLLSYKLPINLKIKYPVSFACITIFIILLLGKNGSA
                     EFIYFQF"
BASE COUNT         1420 a          807 c          801 g         1241 t
ORIGIN      
        1 tgcagaatca gataatgttt acctcgatga gagcgagtta aatgcaatct atcgtcttga
       61 aataacccaa gaacgtatca aaacggaata cccaaactta acgtcagggc aggtgcggag
      121 gaaatacgag agtttgcttg tcgttcgaga ccgattcttg gtaggggcct acaccggatt
      181 gcgagtatct gacttttcac gcatcggaga aatgaatatt gatgaaaact atattcaact
      241 ccgctacaac aacaattggg gcaacttata caaatgcctt tacccagccg atttagaaaa
      301 cctgggcacc aacgctaatg cttataaact tacagcacaa ggtcgcagag tgtatgaact
      361 taaaaccaat acggttcagg ataattatac cgacctgaaa aattttgttc aggcattgca
      421 cgcacccgca acaacaaatt atcaatataa tttagaaaaa cagtttaacg taaattcttt
      481 tttacgtacg tatgcatttg atgtaataac aggcaactgg gataattatg ccggtaacac
      541 caacaactat tatttgtata atgattggca aaccggtaag ataaattacc tgccgtatga
      601 tgttgataat acgtatggca ttgattggtt aggtaaagac tggggcaccc gcaacgtata
      661 taattggccg gttaatagtg ctaataaata tttgattaca aaacttatgg cctatcccga
      721 ttacgctaac cggtttacgt tctacttaaa ccaaattgta gccggtgctg gttcattcgc
      781 atttcaacaa acatttattg ataatataaa aacacaaata gaaacctatg ttattaatga
      841 cacttactac acattagact acggatacac ctttggtgat tttattaact catataccca
      901 agccataggt ggtcatgttg attacggcct aaagccttat ataagcacac gtaatgcaca
      961 agcagtttcg cagatgcaag ttaatcaggt ggcgcccata attagcgaaa aacgacatta
     1021 tccttttaga cctgtagcag gcgatacggt ttttattaaa gcattggtag aagacgaact
     1081 aaccgttgtt aatccggttt taaattaccg ctatggccaa actgcaacct ggcttacaac
     1141 taatatgttt gatgacggtt tgcataatga tgatgcatca ggcgataata tttttggtgt
     1201 ggcacttaat ccggttagca caaaggatac actatactat tacatcactt gtacggatgc
     1261 taacaactta acaggccgcg aaccccgatt atacagcgat actttaatta ttgaaacgcc
     1321 atgggcagtt agcataaacg agtttatggc cgataacgcc aatgtgattg ccgataatta
     1381 tggcgagtat gacgattgga ttgaactgta taatgctggc accactatag tcaaccagca
     1441 attgttttta acggatgatt ttttaaaacc tgataagtgg cgcattacca cacccattgc
     1501 caatggcttt caaatatttt gggctgatga tgatgaacaa caaggcagta atcacgcatc
     1561 gtttaagctc aatgcaacag ccgacaaaat tgccattacg gaatacacag gtaaagattt
     1621 cagatttatt gatagtcttt cttatggtgt gcaaaccacc aatgtaagca tgggttgcta
     1681 tcccgatggg gctaagccaa ttgtaacact tgcatcggtt acacctatgt tttctaatgt
     1741 agtagcagct actttttaca acgaatttaa aaccatagta catcccaatc cggtaaacaa
     1801 tattttatac attaatgcct caacaccgtt aagtagcctg caagtgtttg atgttaccgg
     1861 acgtgtttta ttagagcaaa agatacagca gtcgttgcat ttaattcagc ttgatgtaac
     1921 taacttacct cagggtaatt attttatacg attagttggt gccaacggat tagcaaatgt
     1981 taagttttgt aaacagctgt aactgttacc atatatgtta ccacagtata ttcaaaaaat
     2041 taacacggct aaaagcaaga aaggcgacat taaaaaactg ttagcatttt tacaaagtaa
     2101 aaggccgggc gatttggata cagttaccaa caggttgcat gacgaagctt ttgataatat
     2161 agattgtttg caatgtgcaa actgttgcaa aacaacaggc ccgctgctaa taagcaagga
     2221 tgtaagccga ttagcgcagg ctgtaaaatt agcgcccggt aattttatga atacatacct
     2281 aaaaaccgat gaagatggcg atgttgtttt taaacagatg ccttgcatgt ttttaaacaa
     2341 caacaattat tgtaaggttt accatgccag gcctaatgct tgtagagatt atccgcatac
     2401 ccaacaaaat aaaataatac agaagcttga aatcactttg cacaacagca tgatttgtcc
     2461 tgctgtagcc gatgtagttg ccggcttaaa aaaatactac atgaataagt aattacttaa
     2521 gtaagccgcg tgcttgtaaa ctatctttta actgttccga aaaaactact gctccgttat
     2581 agtttaagtg tgtggtgttt ataaacatac tatcaggata gctgtgatgc atattgatga
     2641 aatgaaccaa cggtatttgc gttatcgaat caataaaggt tttaaatccg gattgatttt
     2701 tataagaaac aaaataactg ttgtgtatag gcgatagcac aaaaactaat ggcgtaactt
     2761 tattggttat taataaattt attaaatcac gctcaagtgc agtatcattt ttaaaagtag
     2821 ttgttgtgtt ttttctttca gctatcagcg atgcgaattt ttgtggcgtg agtacatttt
     2881 tttcgaaatt tccaccacag tctgttacct tggttagttg cagtttgctg tttaaatagt
     2941 atttagtgta cagttcaaag ctgccaaaat atttgaaaaa cggaatggcg aaccaaggct
     3001 cataagcatt gcccataagt tttcttacag gttggtatga agcattataa atatagttac
     3061 ttaaatcgcc caagctgctg tttaagcctt cggggtcgaa attaattata atgggctgtg
     3121 ttgattgttg tttaagagcc tccgataaaa agtaaagcat cacattatcc tgcgcacccg
     3181 acaagccata attaaatgca tgcgaactta gtatcgaagg tataaaagca ccttcggcac
     3241 gtgacgaacc tagtatgctt atttcatttg ccggcctgtg tatgatacga tttactttat
     3301 aagcaccatt actgctggag cctaatatta ttttttgctc gaaaaaataa ttaagtatcc
     3361 cgcccgctgc aattacaatg caagcaaccg gtatcagata cgtttttaaa aacaatttaa
     3421 aactggaagt aaataaactc tgctgagcca tttttgccta ataataaaat gatgaaaatt
     3481 gtaatgcaag caaagcttac cggatattta atttttaagt ttatgggtaa cttataactt
     3541 aacagaagca gtacaattga caagaaacaa agagccaaat taagtggccc tttttcggct
     3601 gtaagttgtg ccatgtttaa cgtgaaatca aaagcaagta atttatgtat tacttgccaa
     3661 gcttgcgata tattttcagc tctgaaaaaa atccatgcaa atgttactgc tataaaaaca
     3721 aacaacaccg aacatgcatg cataattttt gtttgatata agccgatgct ttgccaaaaa
     3781 cgaatgcggg tgttttttgt aaccagttcg aaaatggtgt aacatgcatg caaagcaccc
     3841 caaacaacaa aggtccagtt ggcgccatgc cataaaccac ttactaaaaa tacaacagat
     3901 aaattccggt accatttaat tttactacaa cggttgccac ctaaagggat gtataaataa
     3961 tcgcgaaacc aggtagaaag tgatatatgc cagcgactcc aaaagttggc tatcgaagtg
     4021 gctaaatacg gccggttaaa atttagcatt aactcgtatc ccatcatgcg tgctgcgcca
     4081 cgggcaatgt cggtatagcc gctgaagtcg caataaatct gaaatgaaaa aaatatacaa
     4141 gccaatgcaa cagaaatgct tgatgcatgc tcaatatcgc catacacctg atttacataa
     4201 acagcaagcc tgtcggctac caccaatttc ttaaaaaatc cgtatagcat caacgttaac
     4261 ccctcaaca
//
LOCUS       DAOMAA010000035         4255 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_13834_length_4255_cov_3.657619, whole genome shotgun sequence.
ACCESSION   DAOMAA010000035 DAOMAA010000000
VERSION     DAOMAA010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4255
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13834_length_4255_cov_3.657619"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..120)
                     /locus_tag="PLO59_00935"
     CDS             complement(<1..120)
                     /locus_tag="PLO59_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HQU99684.1"
                     /translation="MKFIVSSSALLKQVQMVSGALNNNSPLDILTHFLFDIDKN"
     gene            336..857
                     /locus_tag="PLO59_00940"
     CDS             336..857
                     /locus_tag="PLO59_00940"
                     /inference="COORDINATES: protein motif:HMM:NF017816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF922 domain-containing protein"
                     /protein_id="HQU99685.1"
                     /translation="MMYNLVAAQSIKWQADVLLTFDDFKSKPKPADKTQAALTASTMV
                     FSYKSNGKTIQFEMYSLFDQNNSWFVEEAKTPAILMHEQMHFNITEWHVRLLRQQLSN
                     MHLSNHYNFANSISKVYKAVQQQCAQMQKKYDKETNHGLDENMQMMWQAKVADALSEL
                     AQFDNEKFEMVID"
     gene            complement(930..2564)
                     /locus_tag="PLO59_00945"
     CDS             complement(930..2564)
                     /locus_tag="PLO59_00945"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU99686.1"
                     /translation="MYTRLNYGGGNPLNHYLIGLGTNHFLTATPCPRPDSVITYLRFT
                     RTSKNGNVIDANTAIAFREKNDTSILYNAAGYGIAEIDATNNKGGYATVGNVISFECD
                     SMDRTDGLFLKLNNAGTASNCFRYDFIGGAEVFTKIIRSQLYSNTYYICGYTTIPSGN
                     GIQAIVLSVNNKGAVNWSRTIDLSPTALGTQSKLFSLAEDLNNGNLFAVGTYYDPTVL
                     SPNTQGLAISISSAGVVNWNKSYNLLHDEFRDVQYDTNSFIITGTSDFNPVPLGFNFN
                     DIWLIKIDTTGTINFSNKLRISDNNGNEYQSRGVDMTVRLSSSNVKEYYITGPAYSNP
                     SKAVVYKCNATGAGLNWYDYHETVYDYGFAIDKSPENATATKGFALFSNITEAPANLG
                     APHANLRRMYFNGVTCLRTCVPNIPNTIAVMPTVTTLTHTQQTQYTKRPLTAFNFTGA
                     THNNCHVNSISCGSNAKTTDYFETDEFTVYPNPASDIINLSGINHYTISITDITGKQV
                     MQVIQNDYETSIDISSLKPGIYFIDCGFNHALFVKR"
     gene            2976..>4255
                     /locus_tag="PLO59_00950"
     CDS             2976..>4255
                     /locus_tag="PLO59_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99687.1"
                     /translation="MEPKPQGINPLIVSDNQLFVDDSLNVYVAGGFVDTALFMMGSWG
                     IAKIDAGGNVLWYAIEPGDGGGSKITSIALDTSCNVYASGNLSTIIGTVAVTQKYNAA
                     GVLQWTRQLDTVGNMGTDCEGILVSADNHCYEHVRRSNITIRRYDALGNVTHQFSNDT
                     INGWQNHAFIASEFKNDNVGNLYVAGTYVDTPNNLERKYFVKKFKPLGGLLWERFYNP
                     TTGSDNPSDLYIDSLQNVYLTGYKNNKVACETVVYDSAGNEKWHQSFQLGGGGWPDEV
                     VADKAGNCYVNGFASLTTAGGGTLIKYDTSGVLLWQRTIDSVAGGSFPVAKVVLDDAA
                     NIYVSYGARNNTVLPKCIVTAKYDSAGNLIWKVYYYPAIPATIVPKALALDKYNNVYI
                     TGEGFVVKYSQTASINNLPQPGKRCFERAIASRQ"
BASE COUNT         1320 a          744 c          866 g         1325 t
ORIGIN      
        1 gtttttgtca atatcaaaca aaaaatgggt aagtatatct aaggggctgt tgttattgag
       61 cgcaccgctt accatctgaa cttgtttaag caaagcactg ctggatacta taaatttcat
      121 gattgtttaa aaactgtgaa tttgaagtgg taatgttttg gcagtaatga taaggcgatt
      181 atcagatagc agaaaaaaat atgcctattt aattataaat ttttataaac atattgcata
      241 tatacggtta tatagcgttt taaatggtta tgtatttaaa agcaaattta aggtttaaac
      301 gattgaaaca tctttggctt tgcagctgtt gcctgatgat gtataatttg gttgctgcgc
      361 aaagcattaa atggcaagcg gacgttttac ttacgtttga tgattttaaa agcaaaccca
      421 aaccggccga taaaacccaa gctgctttaa cagcaagtac gatggttttt agttacaaaa
      481 gcaacggtaa aaccatacag tttgaaatgt atagtttgtt cgatcaaaat aattcatggt
      541 ttgtggaaga agcaaaaacc ccggccatct taatgcatga gcaaatgcat tttaatatta
      601 ccgagtggca tgtacgctta ttgcggcaac aattaagcaa catgcattta agcaaccatt
      661 ataattttgc aaatagtata tcaaaagttt ataaagcggt gcagcaacaa tgcgctcaaa
      721 tgcaaaaaaa gtatgacaaa gaaaccaatc atgggcttga tgaaaacatg caaatgatgt
      781 ggcaagccaa agttgctgat gcattgagtg agttggcgca attcgataac gaaaaatttg
      841 aaatggttat tgattagaaa tgtgtttggt gaaacggttt aaaaagagaa gggcgttttt
      901 taaacgccct tctcttttta aaaatataat taccgcttta caaatagggc atgattaaaa
      961 ccacagtcaa taaagtaaat gccgggcttt aagctgctta tatcaattga cgtttcgtaa
     1021 tcgttttgta ttacctgcat aacttgtttg ccggtaatat ccgttataga tattgtgtag
     1081 tgatttatgc cgcttaggtt tataatatca cttgcaggat tggggtacac tgtaaattca
     1141 tctgtttcaa aatagtcagt tgttttagca ttgctaccac aactaatact gtttacatgg
     1201 cagttgttat gtgtggcgcc tgtaaaatta aaagctgtta atggtcgctt cgtgtattgt
     1261 gtttgctgtg tatgcgttaa tgtagttacc gttggcatta cggctatggt gttgggtatg
     1321 tttggcacac aagttcttaa acaagtaacg ccattaaaat acatgcgcct taaatttgca
     1381 tgtggtgcac ctaaattggc cggtgcttca gtaatgttcg aaaacagtgc aaaacctttg
     1441 gtagcggttg cattttcagg acttttatca atagcaaaac catagtcata aaccgtttca
     1501 tgataatcgt accagtttaa acccgcaccc gttgcattgc atttataaac cacggccttg
     1561 cttgggttgg aatacgcggg gcctgtaata taatattcct taacattgct cgatgacaag
     1621 cgtacagtca tatcaacacc acggctttga tattcgttcc cgttattatc actaatgcgc
     1681 aatttgttac tgaaattgat tgtacctgtt gtgtctatct ttatcagcca tatatcatta
     1741 aaattgaaac ctaatggcac cggattaaaa tcgctggtgc cggttattat aaaactgttt
     1801 gtatcatact gtacatctct aaactcatca tgtaataaat tatagctttt attccagttt
     1861 acaacacctg cactggatat gctaatggct aaaccttgtg tattgggcga taaaaccgta
     1921 ggatcatagt aagtacccac tgcaaacaga ttgccattgt ttaggtcttc ggctaaacta
     1981 aaaagtttgc tttgtgtacc caaagccgta gggcttaaat caattgtacg gctccagttt
     2041 acagcgccct tgttgtttac cgacagtact atggcttgta tgccattgcc cgaaggtatt
     2101 gtagtataac cacatatata gtaagtgtta ctataaagtt gcgagcggat aattttagta
     2161 aacacttcag cgccaccaat aaaatcatat ctgaaacaat tgcttgctgt gcctgcattg
     2221 tttaatttta aaaacaaacc gtctgtacga tccatactat cgcattcgaa cgatattaca
     2281 ttgcctacgg tagcatagcc gcctttgtta ttggtagcat caatttcggc aatgccgtaa
     2341 ccggcagcat tgtataaaat gctggtatcg tttttttcgc gaaatgcaat tgccgtgttg
     2401 gcatctataa cattaccgtt tttactggtg cgtgtaaaac gcaaataagt tattaccgag
     2461 tcggggcggg ggcagggtgt tgccgttaaa aagtgattgg tgcctaagcc tattaaataa
     2521 tggtttaatg gattaccgcc accataattt aagcgagtgt acatgccatc ataaaatgtt
     2581 tcgaacctta ccgtgggatt accactccaa ggcaaataat aattgttgga aaaatattgg
     2641 cccaacaaaa tacaatgacc aagcgtaaag attgcagaaa gtagtaggtg ctttttcata
     2701 ttttataatt ttattaattt gaaataaaat aaaagtttgc gattatcaaa aacatgcttc
     2761 acatataact ttagcgttgt tatatacttt tgtagcaagt ataatttgat tttgcaatgc
     2821 acagatgttt tttagtgctg tgcttgccca ttaaaaataa aatatagatt tactattaca
     2881 aaaagcctaa ccttatgcta cgcatcaatt tgttcttatg cctgctaatt gcgcaaatat
     2941 gggtaaatgc ctaaacggtt acacaggcat ggttagtgga gccaaaacca caaggcataa
     3001 atcctcttat tgtatccgac aatcagctat ttgttgacga tagtcttaac gtatatgttg
     3061 caggaggttt tgtggatact gctcttttta tgatgggcag ttggggtatt gctaaaattg
     3121 atgccggtgg caatgtattg tggtatgcca ttgaaccagg cgatggtggc ggcagcaaaa
     3181 ttacttctat tgcacttgat acaagctgta atgtatatgc ctctggtaat ttatcaacca
     3241 ttataggcac ggttgccgta acacaaaaat ataatgctgc aggggtttta caatggacac
     3301 ggcaattaga taccgttggc aatatgggta ccgattgtga aggcattttg gtttcggccg
     3361 acaatcattg ttatgagcat gtaaggcgga gcaatattac catacgcagg tacgatgcct
     3421 taggcaatgt aacacatcag tttagcaacg ataccattaa tggctggcaa aaccacgcat
     3481 ttatagcctc agagtttaaa aatgataatg tgggtaattt atacgtggct ggcacctatg
     3541 ttgacacccc taacaattta gaaagaaaat attttgtaaa aaaattcaag cccttgggcg
     3601 gccttttatg ggagcggttt tacaatccca ccacgggctc tgacaaccct tccgacttat
     3661 atattgatag tttacaaaat gtttatttaa cagggtataa aaataacaaa gtagcttgcg
     3721 aaactgtagt ttatgacagt gcaggaaatg aaaagtggca tcagtcattt caattgggtg
     3781 gaggcggatg gcctgatgaa gttgtggcag ataaagcagg caattgttat gtaaatggct
     3841 ttgcttcact tacaacagca ggagggggca ccttaattaa gtacgataca tcaggtgttt
     3901 tgttatggca acgtacaatt gattctgttg ccggaggttc atttcctgta gcgaaagttg
     3961 tattagatga tgcagccaac atttatgttt cttatggtgc ccgaaacaat acagtattgc
     4021 ccaaatgtat tgttactgcc aagtatgata gtgcaggcaa cctgatatgg aaagtatatt
     4081 actacccagc tatacctgct actattgtac cgaaagcatt ggcgcttgat aaatacaaca
     4141 atgtatatat aacaggcgaa ggttttgtag taaagtacag ccaaacagca agcattaaca
     4201 atttacccca acccggtaag cgatgttttg aacgtgcaat tgccagcagg caaca
//
LOCUS       DAOMAA010000036         4236 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_13950_length_4236_cov_3.190146, whole genome shotgun sequence.
ACCESSION   DAOMAA010000036 DAOMAA010000000
VERSION     DAOMAA010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4236
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13950_length_4236_cov_3.190146"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1396
                     /locus_tag="PLO59_00955"
     CDS             <1..1396
                     /locus_tag="PLO59_00955"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU99688.1"
                     /translation="ANTVVISGHIYHDANNNGVYDFGETGIPYQPVTIGSSTVYTNSF
                     GEYTSYEAVGTYTITYVPTGVYAGFALSTPGSITVNATTVGNTYWNNDFGLNIPAGSN
                     DLSVNLSPSTNIAPGFSAWYYITVTNTGVLPASGVVTYHYPTGLTFDKSTPNAATVNA
                     TTQTITWNVNNIPAFSSKTIWVDFNASTTLPLGTLMLSMVDVTLNTGTDPNMANNMDS
                     VHQIVVGSWDPNNKLVTRTNLPNVNYQVISSVNPDQSIDYTINFQNEGTAPAVNIVVT
                     DELSSNVTLNSFQFLGTSHNADVKRNGNVVTYVFPNIMLEDKTTNEPMSHGFIKYRIN
                     AVNGLTPGTQISDKAAIYFDFNAPVITNDAIITMILPQGINESTGNAAIQLYPNPVNS
                     ISKVQFDLAKQAHVTVEVIDVTGRLQAVLANGILPAGNQNLILDASKMANGIYTIKAN
                     VDGKLSLVKFTVAH"
     gene            1581..2675
                     /gene="carA"
                     /locus_tag="PLO59_00960"
     CDS             1581..2675
                     /gene="carA"
                     /locus_tag="PLO59_00960"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HQU99689.1"
                     /translation="MKFSSKTPALLVLADGTVFKGNAAGKKGTATGEICFNTGMTGYQ
                     EVFTDPSYYGQILIETNVHIGNYGIRLAESESDSIKIAGLVCKSFHAYYSRKDADTSV
                     TAYFENNNIVAIVDVDTRAVVRHIRSKGAMNAIISSEILDVEILKQKLAEVPSMEGLE
                     LSSTVSTKTAYTAGDENSKLRVALLDLGIKQNTIRCLNERGVFVKVFPYNSSFKTMEA
                     FKPNGYLISNGPGDPAVMPGVVDTVTQIVEAGKPLFGICLGHQMLAESQGIKTYKMFN
                     GHRGINHPVKNVITGRCEITSQNHGFAINEADVRNSSTVEITHLNLNDNTIEGIRVKG
                     AKAFSVQYHPESAPGPHDSRYLFDDFVASL"
     gene            2710..3198
                     /gene="purE"
                     /locus_tag="PLO59_00965"
     CDS             2710..3198
                     /gene="purE"
                     /locus_tag="PLO59_00965"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009284725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HQU99690.1"
                     /translation="MNPVIGIVMGSSSDLQVMQQAADVLKQFNIAFEIKIVSAHRTPA
                     IMLDYADQAFARGIKIIIAGAGGAAHLPGMLASVTTLPVIGVPVKSSNSIDGWDSVLS
                     ILQMPNGVPVATVALNGATNAALLAVQILGTSNLSIHEKLVQYKQQLKDKVKLMNEGL
                     NG"
     gene            3286..4128
                     /locus_tag="PLO59_00970"
     CDS             3286..4128
                     /locus_tag="PLO59_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99691.1"
                     /translation="MEFKNFVVAFIVAVSFQFYCKAGNENMPAGARAAGMGNAAVTLT
                     DMWSAFQNQAGLATLKGLHIGAFNQLRYGQTELMQNALVVALPLKDVGTFAVTFNQFG
                     YSAYNEQKVGIAYARSFGKTVSAAMQLNYNGTNISDNYYGNNHAVTVEAGMRIILLPE
                     LTFGVHVYNPTRAALNNYNDERIPTTFKAGLGYQLNEKVLIAAEIEKTSETKPVFKTG
                     FEYQFVKKMWLRAGFSTNQNQGYFGLGMQLKQLQINFAASFHQTLGFTPHADLGYNFV
                     SANP"
BASE COUNT         1383 a          832 c          826 g         1195 t
ORIGIN      
        1 tgccaacaca gttgtaattt caggtcacat atatcatgat gccaataaca acggtgttta
       61 cgactttggc gaaaccggta ttccatatca gccggttaca ataggcagct caacggttta
      121 taccaactca tttggcgaat acaccagtta cgaagccgta ggtacttata ccattactta
      181 cgtacctaca ggcgtttatg caggctttgc attaagtaca ccgggcagta ttactgtaaa
      241 tgcaacaacg gtaggcaaca cttattggaa taacgatttt ggcttaaata tacctgccgg
      301 ctcaaacgat ttatcagtaa acctttcgcc ctcaaccaac attgcgcccg gttttagcgc
      361 ctggtattat ataaccgtaa ctaacacagg tgtattacca gcaagtggtg tggtaaccta
      421 tcattatcca accggtttaa ctttcgataa atcaacacca aacgcagcta cggtaaatgc
      481 taccacacaa accataacct ggaatgtaaa taacatccct gcatttagca gcaaaaccat
      541 ttgggttgat tttaatgcaa gcacaacttt accacttggc acattaatgt taagtatggt
      601 tgatgtaaca cttaacaccg gtaccgatcc aaatatggca aacaacatgg actctgtaca
      661 tcaaattgtg gtgggttcat gggatccaaa caacaagttg gtaacgcgta ccaatttgcc
      721 caatgtaaac taccaggtaa tatcaagcgt taaccccgat caaagtattg attacacaat
      781 taacttccaa aacgaaggca cagcacctgc tgtaaatatt gtagtaacag atgaattaag
      841 cagcaatgta acattaaaca gctttcagtt tttaggcaca agccacaatg ctgatgtaaa
      901 acgaaacggc aatgtggtaa cctatgtttt ccctaacatt atgttagaag ataaaacaac
      961 caatgagcct atgagtcatg gctttattaa ataccgtatt aatgctgtta atggtttaac
     1021 accaggtaca caaattagcg ataaagccgc gatttatttc gactttaacg caccggttat
     1081 tacaaacgat gctattatta caatgatact tcctcaaggt ataaacgaaa gcacaggcaa
     1141 tgctgccatt cagttatatc caaatccggt taatagcatt tcaaaagttc agttcgattt
     1201 agccaaacaa gcccacgtta cagttgaagt aattgacgta accggacgct tacaagctgt
     1261 attagctaat ggaattttac cggcaggtaa tcaaaaccta atattagatg ccagcaaaat
     1321 ggccaatggt atttatacta taaaagccaa tgttgacggc aaactaagct tagttaaatt
     1381 tacagttgca cattaattat tattaaaatt gtttttaaac ccctgatgca taaatgtatc
     1441 aggggttttt ttatggatgc caacaatatt ttaataagtt tttagatgat gcatctttat
     1501 aaaaaatact tttgacggcc cttaccaacc actaatggct gataagaagc aactaaacct
     1561 tagtaaataa ataattaagc atgaagttta gcagcaaaac ccccgcatta ttagtattgg
     1621 ccgatggtac agtttttaaa ggtaatgcag ccggcaaaaa aggaactgcc acaggcgaaa
     1681 tttgcttcaa tacagggatg acgggttatc aggaagtttt tactgaccca agctattatg
     1741 gacaaatact aatcgaaacc aatgtacata ttggcaatta cggaatacgt ttggccgaaa
     1801 gcgaatcgga cagcataaaa atagccggct tggtttgcaa atcgtttcat gcttattatt
     1861 cgcgaaagga tgcagataca agtgttactg cgtatttcga aaacaataac attgtagcta
     1921 ttgttgatgt tgatacacgt gctgtggtac gccacataag aagcaaagga gcaatgaatg
     1981 ccataattag ttctgaaatt ttagatgttg aaattctaaa acaaaaactg gccgaagttc
     2041 ccagcatgga aggtttagag ctatcgtcaa ccgtatcaac aaaaacagca tatacagccg
     2101 gtgatgaaaa cagcaaacta cgtgtagcat tactcgattt aggcataaaa caaaacacca
     2161 tcagatgctt aaatgaaagg ggggtgtttg taaaagtatt tccatataac agttccttta
     2221 aaacaatgga agcgtttaaa cccaacggat acctgataag caatggcccg ggcgacccgg
     2281 ccgttatgcc cggtgttgtt gatacagtaa cccaaattgt tgaagctggt aaacctttat
     2341 tcggtatttg cttaggtcat cagatgctgg ccgaatcgca aggcattaaa acttataaaa
     2401 tgtttaatgg ccatagaggc attaatcatc cggttaaaaa cgtaataaca ggtcgctgcg
     2461 aaataacttc tcaaaatcac ggttttgcca ttaacgaagc cgatgtgcga aacagcagca
     2521 ccgttgaaat tacacatctt aatttaaacg ataacacaat tgaaggtata cgcgtaaaag
     2581 gtgccaaagc atttagtgta caatatcatc ccgaaagcgc ccccgggcca cacgactcgc
     2641 gttatctctt tgatgatttt gtagcttcgt tataaaccca gttaattcac acactaaaaa
     2701 aacatctaaa tgaatccggt aataggtata gtaatgggta gtagcagcga tttgcaggta
     2761 atgcagcaag ctgccgatgt tttaaaacaa tttaatatcg cttttgaaat taaaattgta
     2821 agtgcacatc gcacaccggc tattatgttg gattatgccg atcaggcttt tgcacgtggc
     2881 attaaaatta ttattgccgg tgccggtggt gccgcacatt tgcccggtat gttggcaagt
     2941 gttaccacat tacctgtaat tggtgtgccg gttaagtcat caaactctat tgatggttgg
     3001 gatagtgtgc tttctatttt acaaatgcct aacggtgtgc ctgtggccac ggttgcctta
     3061 aatggtgcaa caaatgcagc tttacttgca gtacaaattc tgggcaccag taacctgagc
     3121 atacatgaaa aacttgtgca atacaaacaa caactaaagg ataaagtaaa gctaatgaat
     3181 gagggtttaa atgggtaata catttaacct tcgaaaatcc ttttaaccaa aggttgtagt
     3241 taatagttaa atgctttact taggccgtat aactattgcc ccttgttgga atttaaaaat
     3301 tttgtagtag cctttattgt tgccgttagc tttcaatttt attgtaaagc cggtaatgaa
     3361 aatatgccgg ccggtgcccg cgctgcaggt atgggcaacg ctgcagttac tttaaccgat
     3421 atgtggtcgg catttcaaaa tcaggccggc ttggcaactt taaaaggctt gcacattggc
     3481 gcttttaacc aactcagata cggccaaacc gagctgatgc aaaacgcttt ggtagttgca
     3541 ttaccattaa aagatgtagg cacttttgca gttacattta atcaatttgg ttatagcgct
     3601 tataacgaac aaaaagtagg tattgcatat gcacgcagtt ttggtaaaac ggtttcggca
     3661 gctatgcagt taaattataa tggcaccaac attagcgata attactatgg taataaccat
     3721 gctgtaacag tcgaagcggg tatgcgtatc atactgttgc ctgagttaac ttttggcgta
     3781 catgtttata atcctacgcg tgctgcactt aacaactaca atgatgaacg cattcccacc
     3841 acatttaaag caggtttagg ttatcagtta aatgagaaag ttttaattgc tgccgaaatt
     3901 gaaaaaacat ctgaaacaaa gcctgttttt aaaacaggat tcgaatacca atttgtaaaa
     3961 aagatgtggt tgcgggcggg atttagcacc aatcaaaatc agggatattt tggtttgggc
     4021 atgcaactta aacaactaca gataaatttt gcggcatcat ttcatcaaac attaggcttc
     4081 acaccacatg ccgatttagg ttacaacttt gtgtctgcaa atccgtaatg aaacattact
     4141 acctacatag cttatgcctg ttaatttgtt gcgcatgtat gttaaatgtg caagcacagg
     4201 ttacacctgt aaacaacgag caacatctgg acgacc
//
LOCUS       DAOMAA010000037         4205 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_14138_length_4205_cov_2.687470, whole genome shotgun sequence.
ACCESSION   DAOMAA010000037 DAOMAA010000000
VERSION     DAOMAA010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4205
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14138_length_4205_cov_2.687470"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1022
                     /locus_tag="PLO59_00975"
     CDS             <1..1022
                     /locus_tag="PLO59_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99692.1"
                     /translation="FTLDKTSTQQYFITDALLKYSTMHELSKSNLDTIASILNRVSTD
                     TRQGADLISYISASLANHYLAQKNYLMAEICLPNGKHFYKNENQFEAMLSFLSNTNHS
                     VFESFYIKNYPYKANDLVEYKAVQKIKAGQFDAAINLFTSMPESGKKTLPGNPFNIRI
                     NDCHDCDHLAKQNTIFTKQSYCQKMVDILKTANSSNDSNTKSYNYFLYANGLYNQTYY
                     GNGRTITSTFANESGYVAYNPDGYSIGGCQMALKYYQLALNFATNSELKAKYTWMSAK
                     CELNNWYASRAFKQGEKDFEAGKYFKQLKTEFSNTKYMAEVIDECGYFCTYMGKGSKC
                     IKNEY"
     gene            complement(1023..3329)
                     /locus_tag="PLO59_00980"
     CDS             complement(1023..3329)
                     /locus_tag="PLO59_00980"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HQU99693.1"
                     /translation="MSKVKAYHIVGGDFTAEWAGGNDFEVTLKLFRDCAAINGSGFDQ
                     AINIAVYDKVTHIQQQYFVMYLGGVSSLQYAGNLCSPPPQVCVEEGTYIQTVTLPNNP
                     NGYYLIWERCCRNSNVVNLSNPNAAAMAFYLEIADPALQNSTPVFLNDPLPYMCLNQP
                     FNFNFLGYDMDGDSISFAFETPLDGGHTSQSSPNPFSFNGPGAGNLIPPAAPYAFTNW
                     LAGYSTSNIINGNPTLSINPINGQMYAVPTNTGLYAMAVVMYEWRQGVNIGKVRREIE
                     FVVINCNGNVVPNILTSLSQTSSNTFIVNADDTLSFSITASDVKDSLYITHSGNVFAG
                     SGITPPYAISFDTAGLKQVTTQFFWIPSCNHVSASPYTLTYTVTDNGCPLPASKTFDI
                     SILVVPQQAIVPPALLCIEKDMPIPVLHWADTISYNPHFDKAIIYRSTNGAAYYPIDT
                     LYQQQNNTYTDSSANQFQYNYCYYISLFNKCETSITYSDTLCTQQQFNGDVININQVS
                     VSAQNKITIQWPSFAKAAFNTILIYKKETTDTTYYLWKTLPNYLPDSISDSEVNTNAH
                     SYSYIITNKNTCNIPANFGDEGCTILLKGDAIPYRHTIYFTPYRGWLSVKHYEIYRKD
                     DHNRNYQLLNTQNDTSFIDDQLPLLGGQFTYLIKAIADGKPAYSYSNEITLLQQPTVF
                     LPNAFSPNDDTVNDVWKPAVSFVNTFKLLVYNRWGDLVFSAANELSGWNGFSNSKPCD
                     AGIYLYKLLYTGFKGDAYEKTGTITLVK"
     gene            3453..>4205
                     /locus_tag="PLO59_00985"
     CDS             3453..>4205
                     /locus_tag="PLO59_00985"
                     /inference="COORDINATES: protein motif:HMM:NF014047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HQU99694.1"
                     /translation="MKSIQNLLITDIADEGRAVGKFNDLVVFVEKAVPGDVVNVHVFK
                     KNKNFAEAFITEMVQPSAHRIEPFCEHFGICGGCKWQYLTYEQQLFFKQQQVTETLKR
                     LAYISDVPTQNIIASAQTKYYRNKLEFTFSKVKWLTNEERANQESDKVPALGFHIKGR
                     FDKILNIDKCYLQDDLSNQIRNFVREFCIKHNYPFFDLKRQVGLMRNLIIRNSSLGQW
                     MVSVIFSEDVETNRNDLLEALHQKFSQITCLMY"
BASE COUNT         1397 a          730 c          793 g         1285 t
ORIGIN      
        1 tgtttacgtt agataaaact tcaacacaac aatattttat taccgatgct ttgttaaagt
       61 acagcaccat gcatgaacta agcaaatcca atttagacac catagcttct atattaaatc
      121 gcgttagcac tgatacaagg caaggtgctg atttgatttc atatataagt gcttcattgg
      181 ccaatcatta cttggcgcaa aaaaattatt taatggccga aatatgtttg ccaaatggca
      241 aacattttta caaaaatgaa aatcagttcg aagccatgct ttcattttta agcaacacaa
      301 accactcggt attcgaaagt ttttatatta aaaattatcc ttataaggcc aatgatttgg
      361 tagaatacaa agccgtacaa aaaattaagg cagggcaatt tgatgccgct ataaatttat
      421 ttacatcaat gcctgaatcg ggaaaaaaaa cattgcccgg caatccattt aatattcgca
      481 taaacgactg ccatgattgc gatcacctgg ccaagcaaaa tactattttc accaaacaaa
      541 gctattgcca aaaaatggtg gatattttaa aaacagctaa ttccagtaat gattcaaaca
      601 ctaaatccta taattatttt ttgtatgcca atggcttgta caatcaaact tattacggta
      661 atggacgaac aataacttca acatttgcta acgaaagtgg ttatgttgca tataaccccg
      721 atggctatag tattggcggt tgccaaatgg cattaaagta ctatcaactg gcattaaatt
      781 ttgcaaccaa cagcgagtta aaagcaaaat atacctggat gagtgccaaa tgtgaattaa
      841 acaactggta tgcaagccgg gcatttaaac agggcgaaaa agattttgaa gcaggtaaat
      901 attttaaaca gttaaaaacg gaattcagta atactaagta tatggcagaa gttattgatg
      961 aatgtggata tttttgcact tatatgggta agggcagcaa gtgtattaaa aacgaatact
     1021 gattatttaa ccaaagtaat tgttccggtt ttttcatagg catcgccttt aaaaccggtg
     1081 tacaatagtt tgtataaata tatacccgca tcgcaaggct tgctgtttga aaaaccattc
     1141 caaccgctta actcattagc ggcactaaat actaaatcgc cccaacggtt ataaacaagc
     1201 aacttaaagg tgtttacaaa agaaacagcg ggcttccaaa catcattaac agtatcatca
     1261 tttggcgaaa atgcattggg taaaaatacc gttggttgtt gcagtaaagt tatttcatta
     1321 ctgtagctgt atgcaggttt accatcagca atagctttta tcagataggt aaattgtccg
     1381 cctaataagg gcaactgatc atcaataaag ctggtgtcgt tttgtgtgtt taacaattga
     1441 taattacggt tatggtcatc cttccggtaa atttcatagt gcttcacact aagccaaccc
     1501 ctataaggtg taaaataaat agtgtgccgg tatggtatag catcaccttt taataagatg
     1561 gtgcaacctt catcgccaaa gttagcaggt atgttgcaag tgtttttatt tgtaataata
     1621 tagctgtatg aatgtgcatt ggtatttacc tcgctatcgc taatactatc aggcaaataa
     1681 ttaggcagtg ttttccacaa gtaatatgtt gtatcggtgg tttctttttt atatataaga
     1741 atggtgttaa atgctgcttt tgcaaatgat ggccattgaa tagttatttt attctgagcc
     1801 gaaacactaa cctgattaat attaattaca tcgccattaa actgttgctg tgtacacaaa
     1861 gtatcactgt aagtaataga tgtttcacat ttattaaata aagaaatata atagcaatag
     1921 ttgtattgaa actgattggc gctgctatcg gtgtaggtat tgttttgctg ttgatataat
     1981 gtatcaatag gataataagc agcaccatta gttgatctgt aaataattgc tttatcaaaa
     2041 tgtgggttgt atgaaatggt atcggcccaa tgtaaaacgg gtataggcat atctttctca
     2101 atacataaaa gggcaggagg cacaatggct tgctgtggca ctacgagtat gctaatgtca
     2161 aaagttttag aggcaggtaa tgggcatccg ttatctgtta ctgtataggt taaagtatag
     2221 ggtgatgccg aaacatgatt gcaggacggt atccaaaaaa actgtgtggt aacttgttta
     2281 agccctgccg tgtcgaacga aattgcataa ggcggtgtaa taccactgcc ggcaaataca
     2341 tttccactat gtgttatata aagtgagtct ttaacatcgc tggccgtaat actaaaactc
     2401 aaagtgtcgt ctgcattaac tataaaagta ttactgctgg tttggcttaa agatgtaagt
     2461 atgttgggca ctacattgcc attacagtta attacaacaa attcaatttc gcgccttacc
     2521 ttaccaatat taactccttg ccgccattca tacattacta cagccattgc atataaaccg
     2581 gtgtttgttg gtacggcata catttgcccg tttatagggt tgatgcttaa tgttgggtta
     2641 ccattaataa tatttgaagt gctgtaaccc gccagccagt tggtaaatgc ataaggtgct
     2701 gcagggggga ttaaattacc ggcaccgggc ccattaaacg aaaacggatt gggcgatgat
     2761 tgtgatgtgt ggccaccgtc taacggtgtt tcgaaggcaa atgaaattga gtcgccatcc
     2821 atgtcgtaac ctaaaaaatt aaaattaaaa ggttggttta aacacatgta tggcaacgga
     2881 tcgtttaaaa aaacaggagt tgaattttgt aaggcagggt cggcaatttc taagtaaaaa
     2941 gccattgcag cagcattggg gttactcagg tttactacat tgctgttgcg gcaacagcgt
     3001 tcccaaatta agtaataccc attggggttg ttaggtaatg taactgtttg tatataggtg
     3061 ccttcctcta cacaaacttg tggcggtggc gagcataaat tacccgcata ttgtaacgat
     3121 gatacacccc ccaaatacat tacaaaatac tgttgttgta tatgcgttac tttatcataa
     3181 acggcaatat ttattgcctg atcaaaacca cttccgttta tggctgcaca gtctctaaac
     3241 aattttaaag taacttcaaa atcattaccg ccagcccatt ctgcagtaaa atcgcctcca
     3301 acaatatgat atgctttaac cttactcaag tataaaaagc acagcagtat caatgtaaac
     3361 cgtaacatat atgctaatta aagcaatttt taacgcatca catattttgc cttttataat
     3421 tttgcagcct tttgtaaaat aaacacaaca aaatgaaatc aatacaaaat ttactgataa
     3481 ccgatattgc tgacgagggt agggcagtag gcaagtttaa tgatttagtg gtatttgtcg
     3541 aaaaagcggt tcccggtgat gttgtaaatg tgcatgtgtt taaaaagaat aaaaactttg
     3601 ccgaagcatt cattacagag atggtgcaac catcagccca tcgtattgaa cctttttgcg
     3661 aacattttgg catttgtggt ggatgcaaat ggcagtacct tacctatgaa caacaattgt
     3721 tttttaaaca acaacaagta accgaaactt taaaacgttt ggcttacata tccgatgtgc
     3781 ctacgcaaaa tattatagcc tctgcacaaa caaaatacta ccgcaataag ttagagttta
     3841 cattcagcaa agtaaaatgg cttaccaacg aagaacgtgc taaccaggaa agtgataagg
     3901 tacccgcttt gggctttcat ataaaaggca gatttgataa aatacttaat attgataaat
     3961 gttatttgca agatgattta agtaatcaaa tccgcaattt tgtgcgagaa ttttgtatca
     4021 aacataacta cccatttttc gacttaaagc ggcaggttgg attaatgcgt aatttgataa
     4081 tcagaaattc cagtttaggc caatggatgg tatcggttat ttttagcgaa gatgttgaaa
     4141 ccaatcgcaa tgatttatta gaagcgttac atcaaaaatt tagtcagata acctgtttaa
     4201 tgtat
//
LOCUS       DAOMAA010000038         4204 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_14141_length_4204_cov_2.667631, whole genome shotgun sequence.
ACCESSION   DAOMAA010000038 DAOMAA010000000
VERSION     DAOMAA010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4204
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14141_length_4204_cov_2.667631"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..823
                     /gene="floA"
                     /locus_tag="PLO59_00990"
     CDS             2..823
                     /gene="floA"
                     /locus_tag="PLO59_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015264000.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HQU99695.1"
                     /translation="MVVNALINSTKAGLSVTKNEIETHYLAGGHVNNVMKAMISADKA
                     SIPLNFKMATAIDLAGRDVADAVQLSVNPRVIDTPPVSAVAKNGIQLMVKARVTVRAN
                     INQLVGGAGEETILARVGEGIVTTIGSAQDHKSVLENPDQISKTVLNKGLDAGTAFEI
                     LSIDIADIDVGENIGAKLQTDQASADLKVAQAKAEERRAMAVAYEQEMKAKAQEARAK
                     VIEAEAMVPTAMAEAFKNGNLGVMDYYKMQNLQADTGMRDAFGKSNTGSSPEKAK"
     gene            complement(895..2649)
                     /locus_tag="PLO59_00995"
     CDS             complement(895..2649)
                     /locus_tag="PLO59_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008244471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQU99696.1"
                     /translation="MSEVNQKRFNFKLLQQIWVFVKPHHKKLWLALLLTLLLAAIAPL
                     RPYLTQLAIDNLALGSYTLLRYSIFMFGVLLMQAALQFSFSYLANIIAQDAIFDLRAS
                     VFKAISTFKVSYFDGTPVGVLVTRLVSDMETIADIFSDGLLLIVSDIMQMIVIIGFMF
                     YVDWRLALLSLSTLPFLILATRIFQQKIKASFNDVRNQVAALNTFVQEHISGMRIVQL
                     FNHEDVSFKQFEKINKAHRKAHIRSVWYYSIFFPVVEILSALAVALLVWWGASAAIKS
                     NVSFGTITAFIIYVNMLFRPIRELADKFNTLQMGMVSGERVFKLLQQTDKSETSGNVS
                     AKNIKGNIRFENVWFAYKDENWVLRNVTFSVNAGQMIAFVGATGAGKTSIINLLNRYY
                     EIQQGAIYIDDVNIKDYNLMELRQAIGLVQQEVFLFSDSIANNISLFNESISMDEIIQ
                     ASKAIGANNFIEKMPGNYLYNVQERGALLSVGQRQLIAFIRAYIYKPAIFILDEATSS
                     LDSESEVMISQATNAIVHNRTSVVIAHRLSTIQNADVILVMDKGQIIEQGNHETLLTH
                     NGVYKSLYDIQFNTVQPT"
     gene            complement(2680..3456)
                     /gene="truA"
                     /locus_tag="PLO59_01000"
     CDS             complement(2680..3456)
                     /gene="truA"
                     /locus_tag="PLO59_01000"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HQU99697.1"
                     /translation="MRYFLQLQYNGANYAGWQIQNNAVAVQQKLQEALQVLLHQPTNL
                     VGCGRTDTGVHALHFVAHFDTELILNAQSFLFSLNGLLPYDIVVYHLQQVADTAHARF
                     DASSRTYTYYIATQKNAFINKSAWLFNAALDLNAMNAVVPLFLKYNDYSCFSKAHTQT
                     KTNNCVIKYAHWQQQADIICFTITADRFLRGMVRAIVGTMIQVGLHKMDGNSFEQIIK
                     QGKRSMAGKSVPAHGLYLVQVTYPYISFLKPPALSLPTLL"
     gene            complement(3462..>4204)
                     /locus_tag="PLO59_01005"
     CDS             complement(3462..>4204)
                     /locus_tag="PLO59_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99698.1"
                     /translation="YISLNDQQCKIFAEGALNLGTNFGQFASKYAGSITHQMSTDATQ
                     INLTMDCDFPFNSDALKSMADVFSSYPTLKAINEGNAYHKAMNTLVGNEVWAKASAEI
                     AASGSLKKVPDVLKHALIVTDVNLTYNALSKSYRSTGQIGVAFVNKVAIGKYVKGNME
                     VVNKKGSNSFNLYVEIDASTWYFFSFSRNIMQAVSSDATFNEAIANTKDDKRLSKGKD
                     DVPDYQYMLSTERKKNELLKKLIETPEE"
BASE COUNT         1347 a          817 c          766 g         1274 t
ORIGIN      
        1 cattgtggtt aatgcattaa tcaattcgac caaagccggt ctatcggtta ccaaaaacga
       61 aatagaaacg cattatctgg caggtggcca tgtaaataat gttatgaagg ccatgatatc
      121 tgctgataaa gcaagtatcc ctttaaactt taaaatggct acagctattg acttggctgg
      181 ccgcgatgta gccgatgccg tacaacttag tgtaaacccg cgtgtaattg atacgccacc
      241 tgtaagtgct gttgctaaaa atggcataca gcttatggtt aaggcccgag taacggtacg
      301 tgctaacatt aatcaattgg ttggtggtgc cggtgaagaa accatactgg cacgtgtagg
      361 cgaaggtatt gttacaacaa ttggttcggc acaggatcat aaatcggttt tagaaaatcc
      421 ggatcagatt tcgaaaacag ttttaaataa aggacttgat gccggtacag ctttcgaaat
      481 tttatcaatt gacattgccg atattgatgt gggcgaaaac attggcgcca aattacaaac
      541 cgaccaggcc agtgccgatt taaaagttgc acaagccaaa gccgaagaac gcagggcaat
      601 ggccgtagct tacgaacaag agatgaaagc taaggcacaa gaagcgcgcg ccaaagttat
      661 tgaagccgaa gcgatggtac ctacagcaat ggccgaagca tttaaaaatg gcaatttagg
      721 tgttatggat tattataaaa tgcaaaacct gcaagcggat acaggcatgc gcgatgcttt
      781 tggaaaaagc aatacaggaa gcagccctga aaaagcaaaa taaattttta atttttatta
      841 gtttgtatta aaacatttta agctaaccaa tggtatatac gtaacattga ttggttaggt
      901 aggctgcact gtattaaact gaatatcgta caaacttttg taaacgccat tatgtgttaa
      961 gagcgtttca tggttgcctt gctcaattat ttgaccttta tccataacta aaatcacatc
     1021 agcattttgg atggtgctta aacgatgtgc aattaccact gaagtacggt tatgaacgat
     1081 ggcattggta gcctgcgaaa tcattacttc cgactctgaa tcgagcgatg aggttgcctc
     1141 atctaaaata aaaattgccg gtttataaat ataagcacgt ataaatgcta taagttgtcg
     1201 ttggcctacc gataacaagg caccacgctc ctgcacattg tataaataat taccgggcat
     1261 tttttcgata aagttattag ccccaatagc ttttgatgct tgtattatct catccatact
     1321 aatggactca ttaaacaatg aaatattatt ggcaatagaa tctgaaaaca aaaaaacttc
     1381 ttgttgcacc aagcctattg cctgccttaa ctccattaaa ttataatcct tgatattaac
     1441 atcatcaata taaatagcgc cttgttgtat ttcgtaatac cggttgagta aattaattat
     1501 tgaagttttg ccggcaccgg tagctcctac aaaagcaatc atttgacctg catttacact
     1561 aaatgtaaca ttacgcaaaa cccagttctc gtctttatac gcaaaccaaa cattttcgaa
     1621 cctgatattt ccctttatat ttttagctga aacattgccg cttgtttcgc ttttatcggt
     1681 ttgttgcaac agtttaaaaa cacgctcgcc actaaccatg cccatttgaa gtgtattaaa
     1741 tttatcggct aactcgcgta tgggcctaaa caacatattc acataaataa taaatgctgt
     1801 tatagttcca aatgaaacat tgcttttaat tgcagcgctt gcaccccacc aaaccaataa
     1861 tgctactgcc aaggctgaca aaatttcgac aacaggaaaa aaaatactgt aataccacac
     1921 cgaacgtata tgggctttac gatgagcctt gtttattttt tcgaattgct taaacgaaac
     1981 atcttcatga ttaaaaagct gcacaatgcg cataccggaa atatgctcct gaacaaatgt
     2041 atttaaagca gcaacctgat ttcttacatc attaaatgac gctttaattt tttgctgaaa
     2101 tattcgggtg gcaagtatta aaaaaggcag cgtacttaat gataataaag ccaaacgcca
     2161 atccacataa aacataaagc ctataatcac aatcatttgc ataatatccg aaacgataag
     2221 caacaggcca tcagaaaaaa tatcggcaat ggtttccata tccgacacta aacgtgtaac
     2281 cagtacacct accggtgtgc catcaaaata acttacttta aatgtggata tagccttaaa
     2341 cacagacgcg cgcaaatcaa atattgcatc ttgtgcaatg atgtttgcta aatatgaaaa
     2401 tgaaaattgt aatgccgctt gcattaataa cacgccaaac ataaaaatgg aatagcgcaa
     2461 aagagtgtaa ctacctaatg ctaaattatc aattgctaat tgcgtaaggt acggcctcag
     2521 tggtgcaatt gcagccagta gcaaggtaag taataatgcc agccaaagtt ttttatggtg
     2581 tggttttaca aatacccaaa tttgctgcaa cagtttaaaa ttaaaacgct tttgatttac
     2641 ttcactcata tttatgttgc taccacatta aaataaatat tacaataaag ttggtaaact
     2701 taatgcaggc ggttttaaaa aacttatata aggatatgta acctgcacca gatataaacc
     2761 atgtgcaggt acagacttgc cggccatact acgtttacct tgctttatta tttgttcaaa
     2821 actattgcca tccattttat gtaatcccac ctgaatcatg gtgccaacaa tggcacgtac
     2881 cataccacgc aaaaacctat cagctgtaat ggtaaaacaa ataatatcag cctgttgttg
     2941 ccagtgtgca tatttaatta cacaattatt ggttttagtt tgcgtatgag ctttgctgaa
     3001 acaactgtaa tcgttatact ttaaaaacaa aggtacaact gcattcattg catttaaatc
     3061 taaggctgcg ttaaacaacc aggccgattt atttatgaat gcattttttt gtgtggctat
     3121 atagtatgta taagtccggc tgcttgcatc aaaacgcgca tgtgcagtat ctgccacttg
     3181 ttgtaaatga tatactacaa tatcgtatgg taacaagcca tttagcgaaa acaaaaatga
     3241 ttgtgcattt agtattagtt ccgtatcaaa atgtgcaaca aaatgtagtg catgcacacc
     3301 ggtatcggta cgtccacaac ccacaaggtt tgtaggttga tgcagcaata cctgcaaagc
     3361 ttcttgtaat ttttgttgta cagctactgc attgttttga atttgccagc ctgcataatt
     3421 agcaccgttg tattgcagtt gtaaaaagta gcgcataatt tttactcttc gggtgtttcg
     3481 ataagttttt tcaaaagctc attctttttg cgctccgtgc ttaacatgta ttgataatcg
     3541 ggcacatcat cctttccttt gcttaatcgt ttatcgtctt tggtgtttgc aatagcttcg
     3601 ttaaacgtag catcggatga aacagcttgc ataatattac ggctaaatga aaagaagtac
     3661 caggttgatg catcaatctc tacatataaa ttaaagctgt tgcttccctt tttattcact
     3721 acctccatat tgcctttcac atacttacct atggctactt tgtttacaaa tgccacacct
     3781 atttggcctg tagatcggta tgatttgcta agtgcattat aggtcaggtt tacatccgtt
     3841 actatcaaag catgttttaa aacatcgggt acttttttaa gcgatccgct ggcagcaatt
     3901 tcggctgatg ctttcgccca aacttcgtta cctactaacg tattcatggc tttgtgatat
     3961 gcattacctt cgttaatggc tttaagggtt gggtaagatg aaaaaacatc ggccattgat
     4021 ttaagtgcat ccgaattaaa cggaaaatcg cagtccatag tcaaattaat ttgtgtggca
     4081 tcggttgaca tttgatgcgt aatactaccg gcatattttg atgcaaactg gccaaaattg
     4141 gtgcccaggt ttaatgcacc ttcagcaaaa attttacatt gctgatcgtt taaacttata
     4201 tagt
//
LOCUS       DAOMAA010000039         4196 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_14182_length_4196_cov_3.286887, whole genome shotgun sequence.
ACCESSION   DAOMAA010000039 DAOMAA010000000
VERSION     DAOMAA010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14182_length_4196_cov_3.286887"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /locus_tag="PLO59_01010"
     CDS             <1..896
                     /locus_tag="PLO59_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HQU99699.1"
                     /translation="SLVYSVYIICRQLDLSAMAMFEKIFSSEYAGIVVHDIKSGNYFW
                     KNFLGGAAIAIAMTGLDQEMMQKNLSCKNIGEAKKNMFTFSMILVAVNFLFLILGAVL
                     IFYAQQKNITIDAKHTDDLFPTVALQYLGTIPALIFIIGLISAAYPSADGALTALTTA
                     YCFDFLGIQKNNKLSENQRKRIRYTVHISMAVVLLLTIVVFRLINNDAVIRNVFRAAS
                     YTYGPLLGLYAFGFFTRWQIRDTYVWVVCAASPVICYFLYDWVPQVFDGYKFGFELLI
                     VNGLITFLGLTLLIKPAKHVQ"
     gene            complement(871..2223)
                     /locus_tag="PLO59_01015"
     CDS             complement(871..2223)
                     /locus_tag="PLO59_01015"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HQU99700.1"
                     /translation="MKSFLQALFLFLFMLNANTFNLCLAAENKAYVVQINLLDEISTG
                     SWRSFNKAFNYAVAKKADAIVLHLNTYGGMLDAADSIRSKILSSKIPVYAFIDNNAAS
                     AGALISIACNKIFMVNGSSIGAATVVNASGEPLPDKYQSYMRSMMRATAEARQRNPSI
                     AEAMVDPRTFIAGVNDSGKVLTFTVAEALKNNYCDAQVSNLGQLLHAVGIDAYTLDIY
                     HHDWVDKAIAFLLNPAVSGVLILTMLGGLYFELQHPGVGLPLFLAIGAALLYFAPHYL
                     EGLAQNWELLLFIAGLALLALEIFVLPGFGIAGIAAIVCIVLGLALTLVHNDGFDFSV
                     SGTAAIMQALATVVLAKVLVLVLFIFTGERFILSPLFNKLVLTDALESATGYVTSKPV
                     ELIGKQGIAFTDLRPAGKIKINEAIHEASAESNYIPQGTSIEVISHNGVTIIVRALQA
                     "
     gene            2316..3503
                     /locus_tag="PLO59_01020"
     CDS             2316..3503
                     /locus_tag="PLO59_01020"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HQU99701.1"
                     /translation="MRKFLFIYILIFVAAPYLSIADNNNKRVSGQNTPAKYIDGKKHV
                     MHKVAKAETFYSIARLYKIDVKDLMASNKGVSLLKAGQIIQVPVPETYTDLVRTGVEE
                     NAKEITPDEKQTETKPAVYHKVKKGETLSAIAKKYQVQASQIAALNNLKKSQVNINQK
                     LLIKKAELTAVSTATVAPVTQVVPASNIQANTTQTYEHVVKKGETLYGIARTYQVDPK
                     EISRLNNLQNNGIKTGQILVLKKPESFDTATIKTRTNETKIDAPKSAAVETAVDQIVN
                     EPAPAKPTADAITVIDKTPSGKDVKQAIENGEGAWVNDADLNPNKYFALHPSAPIGTI
                     VKVTNKMNGKYVFVKVIGKLPGTGDNENLIIKVSKAAADRMGILDQKFQCELNYAFAS
                     AGN"
     gene            complement(3556..>4196)
                     /locus_tag="PLO59_01025"
     CDS             complement(3556..>4196)
                     /locus_tag="PLO59_01025"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017258066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HQU99702.1"
                     /translation="IASLKKILPESGFIAEEGTEAASNQHLQWIIDPLDGTTNFVHGI
                     PCFCVSIALQAGNEIVVGVVYEINLDECFYTFKDAPSFLNGIEIKVSDTVLMQQSLIA
                     TGFPYTKFERKSAYMEVFEVCMHQTHGLRRLGSAAADLAYVACGRFEAFYEYGLKPWD
                     VAAGVLLVKNAGGLVSDFKGGDNVIFGNDIIASNAGIHQEFLQIVISKFNGA"
BASE COUNT         1391 a          817 c          749 g         1239 t
ORIGIN      
        1 tatctctggt atatagtgtt tatattattt gcaggcaatt agatttaagt gcaatggcca
       61 tgtttgaaaa aatatttagc agtgagtatg caggtattgt tgtgcatgat ataaaatcag
      121 gcaattactt ttggaaaaac tttttgggtg gagcggctat tgctattgcc atgacaggtt
      181 tagatcagga aatgatgcaa aaaaatttaa gctgcaaaaa tattggagaa gctaaaaaaa
      241 acatgtttac gttttcaatg attttagttg ccgtaaattt tctgtttctg attttaggtg
      301 ctgttttaat tttttacgca caacaaaaaa atataaccat tgatgccaaa catactgacg
      361 acttgtttcc aaccgtagcg ttgcaatatt taggcaccat acctgcactt atatttataa
      421 taggtttgat atcggccgca tatcccagtg ccgatggtgc tttaactgct ttaactaccg
      481 cttattgttt cgacttttta ggtattcaaa aaaacaacaa gcttagcgaa aatcagcgca
      541 agcggatacg atacacagta catataagta tggcagtggt tttattgttg accattgtag
      601 ttttcagatt aataaataac gatgctgtta tacgcaatgt gtttagagct gcatcatata
      661 cttatggccc tttattggga ttatatgctt ttggattttt tacacgctgg caaattcgcg
      721 atacatacgt atgggttgtt tgtgcagcat cgcctgtaat atgttacttt ttatacgact
      781 gggtgccaca ggtgtttgac ggttataaat ttggtttcga actgttgatt gtaaacggtc
      841 ttattacatt tttaggattg actttgttaa tcaagcctgc aaagcacgta caataattgt
      901 tacaccgtta tggcttataa cctctatgga tgtaccttgt ggtatataat tactttctgc
      961 actggcctcg tgtatggctt cattaatttt tatttttccg gccggtctta aatcagtaaa
     1021 tgctattcct tgtttcccaa ttaattcaac aggttttgac gtgacatatc cggttgctga
     1081 ttctaatgca tcggtaagaa ctaatttatt aaacaatggc gataatataa accgttcgcc
     1141 tgtaaaaata aacaatacca aaaccaatac tttggctaaa acaactgttg ctaatgcttg
     1201 cattatggct gcagtgccgc tcacactaaa atcaaaacca tcattatgca ccaatgttaa
     1261 tgccaggcct aaaacaatac aaactattgc agctatgccc gctataccaa atccgggtaa
     1321 aacaaaaatc tcaagcgcta atagagccag gccggcaata aataataata actcccagtt
     1381 ttgtgcaagg ccctctaaat aatgaggtgc aaaatacagc aatgctgcgc ctatggctaa
     1441 aaacaaaggc aagcccacac ccggatgttg caactcaaaa tacaaacccc ctaacatagt
     1501 aagtataagc acacccgaca cggcaggatt taatagaaaa gctattgctt tgtcaaccca
     1561 gtcgtgatga tagatatcca atgtgtaggc atcaatacca accgcatgca acaattgacc
     1621 taagttactt acttgtgcat cgcaataatt attttttaat gcttcggcta cagtaaatgt
     1681 aagcaccttg cccgaatcgt ttacaccggc aataaatgtg cgcgggtcta ccatggcttc
     1741 ggctattgac ggatttcttt gtcgcgcttc ggcagtagcc cgcatcatac tgcgcatgta
     1801 cgattgatat ttatcaggca aaggttcacc gcttgcattt acaaccgttg ctgcacctat
     1861 gctactgcca ttaaccataa atattttatt acatgcaatt gaaattaatg caccggccga
     1921 agccgcattg ttatcaataa aagcatacac aggaattttg ctgcttaaga ttttcgaacg
     1981 tatgctatcg gccgcatcaa gcataccgcc ataagtattt aaatgcaata caatggcatc
     2041 ggctttttta gctacagcat aattaaatgc tttattaaac gatcgccacg agccagtact
     2101 tatttcatcc agcaaattta tttgtactac gtaagctttg ttttcggctg ccaaacataa
     2161 attgaaagtg ttggcgttta gcataaataa aaacagaaaa agagcttgta aaaatgattt
     2221 catgttaatt aaagtgttga taaacataaa ggcaaaaatt aatgttttgt ttcaatggct
     2281 ctacttttgc ataatgccct aaaaatggtt aaaaaatgag aaagtttctt tttatataca
     2341 tattaatttt tgttgctgct ccttacctga gtatagctga taacaacaac aagcgtgtta
     2401 gcgggcaaaa tacacctgcc aaatacattg atggcaagaa acatgtaatg cataaagtgg
     2461 ctaaagccga aactttttac agcatagccc gactctataa aatagatgtt aaagatttaa
     2521 tggcgtcaaa caaaggcgtt tctttattaa aagctggtca gattatacaa gtgccggtac
     2581 ctgaaactta tactgatttg gttcgtacag gggttgaaga aaatgccaaa gaaattaccc
     2641 ctgacgaaaa acaaaccgaa accaaaccgg cagtatatca caaagtaaaa aagggcgaaa
     2701 ctttatccgc tattgcaaaa aaatatcagg tgcaggcttc gcaaattgca gcgttaaata
     2761 atttgaaaaa aagtcaggta aacattaatc agaaactact tattaaaaaa gcagaattaa
     2821 cagcggttag cacagccact gttgcacctg taacgcaagt agtacctgca tctaatatcc
     2881 aagctaacac aacacaaact tatgagcatg tggttaaaaa aggcgaaact ctttatggca
     2941 ttgcacgcac ttaccaggta gatccgaaag aaattagccg cttaaataat ttacaaaaca
     3001 atggaattaa aaccggccag atattagtac ttaaaaaacc tgaatcgttt gatacagcaa
     3061 ccattaaaac gcgtactaac gaaacaaaaa ttgatgcccc caaaagtgct gcagttgaaa
     3121 ccgctgttga tcaaattgta aacgaacctg cacctgcaaa acctactgcc gatgcaatta
     3181 cggtaattga caaaacgcca tcaggcaaag atgtaaaaca agcaattgag aatggcgaag
     3241 gcgcttgggt taatgatgcc gatttaaatc cgaataaata ttttgcacta catccatcgg
     3301 cacctatcgg aaccattgta aaagttacca ataaaatgaa tggcaaatat gtatttgtta
     3361 aggttattgg caaattaccc ggtactggtg ataatgaaaa tttaattatc aaagtatcaa
     3421 aagctgctgc agatcgtatg gggatattgg atcaaaaatt tcaatgcgaa cttaattatg
     3481 catttgcatc ggccggtaat taaaagtaac agttcgaata aatgtaaaaa gggttgcttt
     3541 aaaagcaacc cttttttatg cgccattaaa tttacttatt actatttgca aaaactcctg
     3601 atgtatgcct gcatttgaag ctatgatatc gtttccaaaa attacattat cacctccttt
     3661 aaaatcagaa accaaaccac cggcattttt tactaataaa actccggctg ctacatccca
     3721 tggttttaat ccatattcat aaaacgcttc aaaacgacca caagccacat aggccaggtc
     3781 ggctgcagcg ctacccaatc tgcgcaagcc atgtgtttga tgcatacata cttcaaacac
     3841 ttccatatag gcactttttc gttcaaattt tgtgtaggga aaaccggtag ctatcaacga
     3901 ttgttgcatt aaaacggtgt ccgatacttt tatctcgatt ccatttaaaa aactgggtgc
     3961 gtctttgaaa gtgtagaaac attcatccag attaatttca taaacaacac ccacaactat
     4021 ttcattacct gcctgcaatg caatacttac gcaaaaacaa ggtatgccgt gtacaaaatt
     4081 agtagtgcca tctaatggat caattatcca ctgaaggtgc tgattgcttg cagcctcagt
     4141 accttcttcg gcaataaagc ccgattcggg cagaattttt tttaacgaag ctatta
//
LOCUS       DAOMAA010000040         4148 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_14462_length_4148_cov_2.451747, whole genome shotgun sequence.
ACCESSION   DAOMAA010000040 DAOMAA010000000
VERSION     DAOMAA010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4148
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14462_length_4148_cov_2.451747"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1462
                     /locus_tag="PLO59_01030"
     CDS             <1..1462
                     /locus_tag="PLO59_01030"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU99703.1"
                     /translation="AGASATYTFNVVEDAIWNNTELAAIAFVQNDNTKEIYNSGASWD
                     PAVNASLVGPAVVAAQGAVGLVSSFSGVVGNTGNASEDFLFTLSAANAPANWTANFKA
                     NGTTYTAPTTLTLTAGSTINADIEVTPGGTPGLAEYTLTITSVTNPTAPAMFYTVYVI
                     EGVTTLLVNNSSGLGDGSGGNASNWSIDFENGLNFANCSTYAVTNEIVALKVADAGAL
                     NTLHKVFFNVGWTFPSLTDDLVTKLALMLDNGGRLFISGQDIAWETFDPSSGYGTPAT
                     QAFFTNYLNASFVNDGTTSANVALNFNAGDVIFGGTPNSTLINYYGGTYFYPEEINAT
                     GNGAVISYYGAGTAKKGAVRATNGIYKTVYLGYGLEMVGSTAIKNEIIKRAYDWFDNL
                     TSTEEFDAAMLALNIGQNYPNPANQNVIIPVSNLLHDATLQVTDFTGRVVLEQAVNKQ
                     STEVKLNTQNLNAGLYSYRLMVDGKPTQAKTMQVIR"
     gene            1763..2428
                     /locus_tag="PLO59_01035"
     CDS             1763..2428
                     /locus_tag="PLO59_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018480000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQU99704.1"
                     /translation="MIKINNITKSYTIANTSMQVLKGIDLEIKEGEFVSIMGASGSGK
                     STLLNILGILDNYDTGDYYLADTLIKNISESKAAHYRNRMIGFVFQSFNLVAFKNAAE
                     NVALPLYYQKVSRKKRNTLALSYLDKVGLADWAHHLPSEMSGGQKQRVAIARALITQP
                     KIILADEPTGALDSQTSHEVMDLLTEVNRQGITVIIVTHELDVAQRTHRIIRIKDGLI
                     VNE"
     gene            2453..3682
                     /locus_tag="PLO59_01040"
     CDS             2453..3682
                     /locus_tag="PLO59_01040"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQU99705.1"
                     /translation="MFDLDKWQEIFHTIGKNKLRTFLTAFSVAWGIFILIVLLGAGRG
                     LRNGAEHQFLSDAVNTIWINGGTTTMPYQGLQAGREIILKNDDFDDVKNKIEGVDLIS
                     GSARERNQRILSYGKQHLSFTVRSAFADHKDLENAKLISGRFINDIDIAQNRKVCVIG
                     YPVRDALFLKEDPVGKFIDAEGISFKVVGVFTDPGRGDLDRIYIPVTTSQRAYNGQNK
                     LGTIWVSTGNVSLARSMQMCDEIRAMLAKKHHFDPKDQRAINVWNNNQNYVRVMGMLD
                     GIKWFVAVIGVGTLFAGIVGVSNIMMIVVTERTKEIGIRKALGATPVSIITLIVQEAI
                     FITAIAGFAGLFTGVALLELANNYMPPSDFFRKPEVNINIALYATLLLVICGAIAGLV
                     PAMRAAKIQPIVALRDE"
     gene            3714..>4148
                     /locus_tag="PLO59_01045"
     CDS             3714..>4148
                     /locus_tag="PLO59_01045"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQU99706.1"
                     /translation="MFDKDNWQEIFAAIKKNKLRTFLTALGVFWGIFMLVIMLGSGAG
                     LSNGITQGFEGTATNSFFVWTQKTSKAYKGMKPGRIFEFNLADANAIAHNIPEVETVA
                     PQNQLGDYQGVNNVVRGLRTGGFAVNANYPVIQKINSIKIANG"
BASE COUNT         1290 a          819 c          862 g         1177 t
ORIGIN      
        1 agcaggtgca tcggctactt atacttttaa tgtagttgaa gatgctatat ggaacaatac
       61 cgaacttgca gctattgcct ttgttcaaaa cgataataca aaagaaattt acaacagtgg
      121 cgctagctgg gaccctgccg ttaatgcaag tttagtaggg cctgctgtag ttgctgcgca
      181 aggcgctgtg ggtttggttt catcgtttag cggtgtggtt ggcaataccg gcaatgcatc
      241 tgaagatttt ttgtttacgc ttagtgcagc taatgccccc gccaactgga ctgctaactt
      301 taaagcaaac ggcaccactt acactgcacc cacaacgcta acgcttactg caggttcaac
      361 cataaacgct gatattgaag ttacgcccgg tggcacaccc ggccttgccg aatacacact
      421 tacaattaca tcggttacca atccaaccgc ccctgctatg ttttatacgg tttatgtaat
      481 tgaaggtgtt actactttgt tggtaaacaa ctcatcaggc ttgggcgatg gttcgggtgg
      541 taatgcatca aactggagta ttgattttga aaacggatta aactttgcaa actgctcaac
      601 ctatgccgta accaatgaaa tagttgcact taaagttgcc gatgcaggcg ctttaaatac
      661 tttacataaa gtgtttttca atgtgggctg gacattcccc tctcttactg atgatttggt
      721 aaccaaactt gcattaatgt tagataatgg cggccgtttg tttatttcgg gtcaggatat
      781 tgcctgggaa acttttgacc cgtcaagcgg ctatggaaca ccggctacgc aagcattttt
      841 taccaattac ttaaacgctt catttgtaaa cgatggaacc acatcggcta acgtagcatt
      901 aaactttaat gcaggcgatg taatttttgg cggtacacca aacagtacgc tgataaacta
      961 ttatggtggc acttattttt atcctgaaga gataaatgcc accggtaatg gtgccgttat
     1021 ttcttactat ggagccggta ctgcaaaaaa aggcgctgtg cgtgcaacca atggaattta
     1081 caaaacagtt tatttgggtt atggattaga gatggtagga agtacggcca ttaaaaacga
     1141 aataataaaa cgcgcttacg attggtttga taacttaacc agcaccgaag aatttgatgc
     1201 agccatgttg gctttaaata taggtcaaaa ctatcctaac cctgctaatc aaaacgtaat
     1261 tattccggta agtaatctgt tacatgatgc aaccttacag gttactgatt ttacgggccg
     1321 tgttgtgtta gagcaagctg ttaacaaaca aagcactgaa gtaaaactga acacgcaaaa
     1381 tttaaatgcc ggtttatatt cataccgctt aatggttgat ggcaaaccta cacaggctaa
     1441 aaccatgcaa gtgatacgct aaactatttt ttgttttgct atacaaaagg ctgttccaat
     1501 ggaacagcct tttgtcattt atagtaactg ttacaaaact aaaaccaacg gttactacat
     1561 catgtcattg aactataccc taaacatttg caaccggata aaagccaaaa tgtacatagc
     1621 gtaataaagg ttgtaactaa ttaagccgtt gcacgtcaca aaggtttgtt aaagctatgc
     1681 aatgccacat agcagttaac aattgttaac ttctaatgcc aaaaccaagt cttagttttg
     1741 ataatcctca cagcctaaac ttatgattaa aataaacaac ataaccaaat catacactat
     1801 tgccaatacc agtatgcagg tattaaaagg catagattta gagataaaag aaggtgagtt
     1861 tgtttcaatt atgggcgcat cgggttcggg caaatctacg ttgctaaaca tattaggcat
     1921 tttggataat tacgatactg gcgattatta tttagccgac acgcttataa aaaacatcag
     1981 cgaaagcaag gctgcacatt accgcaaccg gatgattggt tttgtgtttc aatcgtttaa
     2041 tctggtggct tttaagaatg ctgccgaaaa cgtagcactg ccattatact atcagaaagt
     2101 aagccgcaaa aaacgcaata cacttgcatt atcttatctt gataaagttg gcctggccga
     2161 ttgggcacat catttaccca gcgaaatgag tggcggacaa aaacaacgtg tagccatagc
     2221 ccgtgcttta attacacagc ccaaaattat tttagccgat gagcctaccg gtgccctaga
     2281 ctcgcaaaca tcgcatgagg taatggattt acttaccgaa gtaaataggc aaggcataac
     2341 ggtaattatt gtaacacatg aattggatgt ggcacaacgc acccaccgta ttattcgcat
     2401 taaagatgga ttaattgtaa atgagtaatg taaaacccta aaccgcaaaa ccatgtttga
     2461 tttagataaa tggcaggaaa tatttcacac catcggcaaa aacaagctac gtactttttt
     2521 aacagccttt agtgtagcct ggggtatatt tatattgatt gtgttattgg gtgcaggccg
     2581 tggtttacgc aacggtgccg aacatcagtt tttaagcgat gctgtaaata ccatttggat
     2641 aaatggcggt accactacca tgccttatca gggattacaa gcaggccgcg aaataatttt
     2701 aaaaaacgat gattttgatg atgtgaaaaa taaaatcgaa ggtgttgatc tgatatcagg
     2761 ttcggcccgc gaacgtaatc agcgcatact gtcttatggc aaacaacatt tatcttttac
     2821 cgtacgcagt gcctttgccg accataaaga cttagaaaac gcaaaattaa tttcgggcag
     2881 gtttattaat gatattgaca ttgcacaaaa tcgtaaagtg tgtgtgatag gctatccggt
     2941 gcgcgatgcg ctttttttaa aagaagaccc tgttggaaaa tttattgatg ccgagggtat
     3001 ttcatttaaa gtagtgggtg tatttaccga ccccggccgt ggcgatttag atcgcatata
     3061 tatacccgtt accacatcgc aacgagctta caatggtcaa aacaaattag gtactatttg
     3121 ggtttcaaca ggcaatgtat ccttagcacg cagcatgcaa atgtgcgatg aaatacgagc
     3181 catgcttgct aaaaaacatc atttcgaccc caaagaccaa cgtgccataa atgtgtggaa
     3241 caacaatcaa aactatgtac gtgtaatggg tatgttagat ggcataaagt ggtttgtagc
     3301 cgtaataggt gtaggtacgc tgtttgccgg cattgtgggt gtaagtaata tcatgatgat
     3361 agtggttacc gagcgtacca aagaaattgg catcagaaaa gcattaggcg ctacacccgt
     3421 aagcattatt acactaattg tacaggaagc catttttata acggccattg ctggttttgc
     3481 aggcttgttt acaggtgttg ccttacttga acttgcaaat aactacatgc cgcccagcga
     3541 ttttttccgt aagcctgaag taaacataaa cattgcatta tatgcaacac tgcttttggt
     3601 tatttgtggt gctattgccg gtttagtgcc tgctatgcgt gcagcaaaaa ttcaaccgat
     3661 agtagctttg cgtgacgaat aataacatca aacttaatta aacccacaac actatgtttg
     3721 ataaagacaa ctggcaagaa atatttgccg ccattaaaaa aaacaagctc cgtactttcc
     3781 taacagcttt aggtgtgttc tggggtattt ttatgctggt aatcatgtta ggctcagggg
     3841 cagggttgag caatggtata actcaagggt ttgaaggcac agccaccaat agtttttttg
     3901 tgtggacaca aaaaacttcc aaagcctata aaggcatgaa acccggtcgc atctttgagt
     3961 ttaacctggc cgatgcaaat gccatagcac acaacattcc cgaagttgaa accgttgctc
     4021 cacaaaatca attaggcgat taccagggtg taaataatgt agtacgaggc ttacgtaccg
     4081 gtggttttgc tgtaaatgcc aactatcctg tgatacaaaa aatcaactca attaaaattg
     4141 caaacgga
//
LOCUS       DAOMAA010000041         4079 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_14924_length_4079_cov_3.398360, whole genome shotgun sequence.
ACCESSION   DAOMAA010000041 DAOMAA010000000
VERSION     DAOMAA010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14924_length_4079_cov_3.398360"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="PLO59_01050"
     CDS             <1..220
                     /locus_tag="PLO59_01050"
                     /inference="COORDINATES: protein motif:HMM:NF017266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MnmC family methyltransferase"
                     /protein_id="HQU99707.1"
                     /translation="DLIFYDAFSPMQQPELWTFEVLHKIYEACNPAAILVTYCAADEV
                     KRTLEKIGFTVENLIGVNGKKQMIRATK"
     gene            230..3904
                     /gene="metH"
                     /locus_tag="PLO59_01055"
     CDS             230..3904
                     /gene="metH"
                     /locus_tag="PLO59_01055"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015030468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HQU99708.1"
                     /translation="MKNIKNELKNRILIIDGAMGTMIQQYKLNEADYRGQRFSNWASD
                     LKGNNDLLSITQPQIIEAIHLEYLNAGADIIETNTFNAQKISLADYQMESLAYELNYE
                     SAKIARQAVSKFANAQSDNNATKYVAGAVGPTNRTLSLSPNVNDPGYRAVTWDEVVEA
                     YTEQLNGLIDGGVDLILVETIFDTLNAKAALYAVQTVFEQKGKQLPVMISCTITDVSV
                     ITLSGQTVEAFLNAVAHIDLLSVGLNCALGAKEMRPYLEELSEKAPFFISAYPNAGLP
                     NQFGEYDETPHQMCEQIDDFLQSGFINIVGGCCGTTPEHIKHIAQHAQKYKPRKIPVI
                     EKYLRLSGLEALTFRPDSNFLNVGERTNVTGSKKFLRLIKDDKYEEALSIARQQVEGG
                     AQAIDINMDEGMLDANQAMTKFLNLIASEPDISKVPVMIDSSKFEAIEAGLKCLQGKG
                     IVNSISLKGGETQFINEANIIKKYGAAVIVMLFDEQGQADTTPRRIEIAKRAYDILVD
                     KINFPAADIIFDPNIFPIATGMEEHRKNAISYFEATKWIKENLPYAKVSGGVSNVSFS
                     FRGNDHVREAIHAAFLYHAIAHGMDMGIVNPSQLIVYDEIEKDLLVLVEDVLLDRNED
                     ATERLIAHAEKIKGSQNSGSAQKERETEAWRNSSVEERIAHSLVKGITDYIDADIEEA
                     RQIFSKPLEVIEGPLMAGMNVVGDLFGAGKMFLPQVVKSARVMKKAVAYLQPFLEKDK
                     AKASAAGRVLLATVKGDVHDIGKNIVGVVMACNGYEIVDMGVMVPAEKILAKAKDENV
                     DVIGLSGLITPSLDEMVHVAKEMTREGFKIPLLIGGATTSKVHTAVKIEPHYAHTVVH
                     VNDASRSVPVVNNLLSQDLREKFIADVKAENARTREYHKADRAKSNYISIAKARANKF
                     ELNAASINEPMCLGVTVFDNYDLNELIPFIDWTPFFVSWEMKGSYPKILKDPVRGTEA
                     QKLFDDAQNMLQLIVAEKWLTAKAVIGIYPAQASGDDILVFDPTTRQSVSNEPLATFY
                     TLRQQTQKPTGYKNVALADFVKPENDYIGAFALSTGFGIEKWIEKFEANHDDYSSIML
                     KALADRLAEAFAELMHKKVRTQLWGYAKDEQLSNEEINKEKYQGIRPAPGYPAQPDHT
                     EKITLWKLLEVEKNTGIKLTESLAMYPTAAVSGLYFAHPDAHYFSVGKLEKDQVEDYA
                     KRKAMTTETIEKWLGSVLAY"
BASE COUNT         1385 a          740 c          881 g         1073 t
ORIGIN      
        1 tgatttaatt ttttacgatg cattttcgcc catgcaacaa cccgaattat ggacctttga
       61 agtgttgcac aaaatttatg aagcatgtaa cccggctgcc attttagtta cttattgtgc
      121 tgcagacgaa gtaaaacgaa ctttagaaaa aattggattt acagttgaaa acctgattgg
      181 tgtaaacgga aaaaagcaaa tgataagggc aaccaaataa aatggattta tgaaaaacat
      241 taaaaacgaa ttaaaaaacc gcatactgat tattgatggt gcaatgggca ccatgattca
      301 acaatacaaa cttaacgaag cagattaccg tggccagcga ttcagcaatt gggcaagcga
      361 cttaaaaggc aacaacgatt tgctttctat tacacagccg caaattattg aagctattca
      421 tttagagtac ctgaatgccg gagccgacat aattgaaacc aataccttta atgcacaaaa
      481 aatttcattg gccgattatc aaatggaatc tttagcttat gagttaaact atgaaagcgc
      541 taaaattgcc aggcaagctg tttctaagtt cgctaatgcc caatccgata acaacgctac
      601 taaatatgtt gccggtgccg ttggcccaac caaccgtaca ttatcacttt cgcctaacgt
      661 aaacgacccc ggctatcgtg ctgttacatg ggatgaagta gtggaagcat ataccgagca
      721 attaaatggt ttgattgatg gcggtgtgga tttaatttta gttgaaacca ttttcgatac
      781 cctgaatgca aaagctgcac tttatgctgt gcaaaccgtg ttcgaacaaa aaggcaaaca
      841 actgcctgta atgatttcgt gcaccattac cgatgtaagt gtcattacac taagtggcca
      901 aaccgtagag gcatttctaa acgctgtggc acatattgat ttattaagtg tgggactaaa
      961 ttgtgcacta ggcgcaaaag aaatgcggcc gtatttggaa gaactaagcg aaaaggcacc
     1021 gttttttatt agtgcatacc ccaatgccgg cttgcccaat cagtttggcg agtatgatga
     1081 aacaccacat caaatgtgtg agcagattga tgattttttg caaagtggtt tcataaatat
     1141 tgtagggggt tgctgtggta ccacacccga acatattaag cacattgcac aacatgcaca
     1201 aaaatataaa ccccggaaaa tacctgtaat cgaaaaatat ttacgactaa gcggcttaga
     1261 agctttaacc ttcaggcccg acagtaattt tttaaatgta ggcgagcgca ccaatgtaac
     1321 cggcagcaaa aaatttttgc gcttaattaa agatgataaa tacgaagaag cattaagcat
     1381 agcaaggcaa caagttgaag gaggtgcgca agcaattgat attaatatgg acgaaggcat
     1441 gttagatgcc aatcaggcca tgactaaatt tttaaacctg atagcttctg aacccgacat
     1501 ttcgaaagtg ccagtaatga tagattccag taagttcgaa gctattgaag cgggcttaaa
     1561 atgtttgcag ggcaaaggca ttgttaactc catttcatta aagggtggcg aaacgcaatt
     1621 tataaacgaa gcaaacatta taaaaaagta tggtgcagcc gttattgtaa tgctgtttga
     1681 cgagcagggc caggccgata ccacaccacg aagaatagaa atagcaaaac gtgcctatga
     1741 tatactggta gataaaataa attttccggc tgccgacatc attttcgatc ccaatatttt
     1801 tcccatagct acaggcatgg aggagcaccg caaaaatgcc attagttatt ttgaagccac
     1861 caaatggatt aaagaaaact tgccttacgc aaaagtttcg ggtggtgtaa gtaatgtgtc
     1921 gttctcgttc cgtggtaacg atcatgtacg cgaagctata catgcagcat ttttatatca
     1981 tgccatagcg cacggtatgg atatgggcat agtaaaccca tcgcaactta tagtgtatga
     2041 tgaaattgag aaagatttat tggtactggt tgaagatgtt ttacttgaca ggaatgaaga
     2101 tgcaacggaa cgattgatag cacatgccga aaaaataaaa ggatcacaaa attcgggttc
     2161 ggctcaaaag gaacgcgaaa ccgaagcctg gcgaaacagc agtgtagaag aacgtatagc
     2221 acattcgtta gtaaaaggta taaccgatta tattgatgcc gacattgaag aagcacgtca
     2281 aatatttagt aaaccacttg aagtaattga agggccttta atggccggta tgaatgtagt
     2341 tggcgattta tttggtgccg gtaaaatgtt tttgccgcag gtggtaaaat cggcacgggt
     2401 aatgaaaaaa gccgtagcat acttgcaacc ctttttagaa aaagataaag caaaagcatc
     2461 ggctgcaggc cgtgtattgc ttgcaaccgt aaaaggcgat gtgcatgata taggtaagaa
     2521 tattgtaggt gttgtaatgg cttgtaatgg gtatgagata gttgatatgg gcgttatggt
     2581 accggccgaa aaaatattgg ccaaagccaa ggatgaaaat gttgatgtga ttggcttaag
     2641 tggtttaatt acaccatcgc tcgatgaaat ggtacatgtt gctaaagaaa tgacacgcga
     2701 aggctttaaa attcctttac taattggcgg tgcaacaacc agcaaagtac atacagccgt
     2761 taaaattgaa ccacattatg cacacaccgt agtgcatgtt aacgatgcat cgcgcagtgt
     2821 gccagtggta aataatttat tgtcgcagga tttaagagaa aagtttattg ccgatgtaaa
     2881 ggccgaaaat gcacgcacac gcgaatacca caaagccgac cgagccaaaa gcaattacat
     2941 ttcaattgct aaggcaagag caaacaaatt tgagttaaac gcagcaagca ttaacgaacc
     3001 aatgtgtttg ggggttacgg ttttcgataa ctacgattta aatgaattga taccgtttat
     3061 tgactggaca cccttttttg tgagttggga aatgaaaggc agttacccta aaattttgaa
     3121 agacccggtg cgcggtaccg aagcacaaaa gctttttgat gatgcacaaa atatgctgca
     3181 acttattgtt gccgaaaaat ggctaacggc caaagcagta ataggtattt atccggctca
     3241 ggcatctggt gatgatatat tggtttttga cccaacaaca aggcaatcag tttctaacga
     3301 acctttagcc accttttata ctttacgaca acaaacacaa aagcccaccg gttataaaaa
     3361 tgtggcattg gccgattttg taaaacctga aaacgactac ataggtgcat ttgctttatc
     3421 taccggattt ggaatcgaaa aatggattga aaaatttgaa gccaatcatg atgattattc
     3481 gtctatcatg cttaaagctt tagccgacag gttggccgaa gcttttgccg aactgatgca
     3541 taaaaaagtg cgtacacaac tttggggcta tgcaaaggat gagcaacttt cgaacgaaga
     3601 aattaataag gaaaagtatc agggcataag gcctgcaccg ggatatccgg cacagcccga
     3661 tcacaccgaa aaaataacac tgtggaaatt gttagaggtc gaaaaaaata ccggaataaa
     3721 acttaccgag agtttagcca tgtatccaac ggctgccgtt agtggtttgt actttgcaca
     3781 tcctgatgca cattattttt cagtaggcaa attagaaaaa gatcaagttg aagattatgc
     3841 caaaagaaaa gcaatgacaa cagaaaccat tgaaaaatgg ttgggaagtg tgttagcata
     3901 ctgatttttt atttatttgt atttcataaa tgaaataata tgtttaggaa aatgtgaata
     3961 tgcaattaaa gctatgattt ttatcgcaca aaaatcacaa cccgaaacgc gcataagcat
     4021 taaagaaata gctggcggta taggcgcacc cgaacatttt attgcgaaaa ttatgcaag
//
LOCUS       DAOMAA010000042         4051 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_15095_length_4051_cov_3.276276, whole genome shotgun sequence.
ACCESSION   DAOMAA010000042 DAOMAA010000000
VERSION     DAOMAA010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4051
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15095_length_4051_cov_3.276276"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            257..1513
                     /locus_tag="PLO59_01060"
     CDS             257..1513
                     /locus_tag="PLO59_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQU99709.1"
                     /translation="MLFFKLFKESIWFALHALRVNKLRTFLSLLGVSVGIFAIIAVFT
                     FVDAMQKSVSDSIKSLGDNTLYVQKWPWTFTDPNYAWWKYFKRPLPDIKEVAQLKLRT
                     TSFKALVFQASSNNQIVKFKNNTVEGSSLLFADYDFNQVSSFEISNGRYFTPIESDAG
                     RPVCIIGYEIAKNLFPFSNPVGQQVQAKGRKLTVVGTFKKAGLNAMGEDYDNVLLAPI
                     NFGSNILDLKSEMLDPFIYAKGIDGISNEALIDELRAAMRNVRRLRPTDEDNFAINES
                     KMLIQSVTGIFDVLQLTGWIIGGFSILVGGFGIANILFVSVKERTSLIGIQKSLGAKN
                     YFILLQFLIEGVVLCLIGGIVGLVLVYLSTMGLAAADFLPFEVSLTLSNIVLALTVST
                     QIGLLAGFVPAYTASQLDPVEAIRSN"
     gene            1535..1879
                     /locus_tag="PLO59_01065"
     CDS             1535..1879
                     /locus_tag="PLO59_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99710.1"
                     /translation="MATVLHKRPLLLSVVCIVNFVLLVAGFMLVSSPFVRNLGVWFPA
                     IYGTLVLTKFAGTVGVWYQKKWGVWLFAASYIVTQFVDVFIDNVSWFGLFYYAGAAII
                     FFCFYNKMDGNL"
     gene            2020..3285
                     /locus_tag="PLO59_01070"
     CDS             2020..3285
                     /locus_tag="PLO59_01070"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012930027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HQU99711.1"
                     /translation="MQVYKFGGASVKDADAIKNVAAIVSAATQPLVVVVSAMGKTTNG
                     LEAVVNAGFYKNGDSKSLLDAVIKFHQDILQQLFEATDDVFTKVNNFWVEIAWALEEE
                     PRSYGFLYDQIVSIGELVATTIIAAYLNRQGTATQWLDARSLIFTDNNYRQAQIQWPQ
                     TVLQINQIIAPVCNQNKVALTQGFIGGTSENYTTTLGREGSDYTAAIIAYCLDAQSVT
                     IWKDVSGVLNADPKFFSDAQKLEQLSYHDAIELTYYGATVIHPKTIKPLENKGIPLYV
                     KSFVKPEGVGTVIGKDLQTKPFIPSYIFKSNQVLISIGSKDFSFIAEAGLSHLFGLFA
                     AAGVKINLMQNSAISFSVCVDDDAFALPALLESLKADYRVLFNNGLVLYTIRHYNQFT
                     IDKLLQKGTLILEQRSRNTAQLIMYPNAD"
     gene            3343..>4051
                     /locus_tag="PLO59_01075"
     CDS             3343..>4051
                     /locus_tag="PLO59_01075"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HQU99712.1"
                     /translation="MQTNNAKPAITSSQRALYYWLLTGCLLIFLMVVIGGITRLTGSG
                     LSITDWKIVTGTLPPLTDSQWQQEFMSYQQTPQYKLVNAHFTLTDFKGIYWWEYLHRL
                     IGRLIGLVFIIPFIIFYKRKWVSSKLLPHLVVIFLMGGFQGFLGWYMVKSGLLSQPSV
                     SHIRLAMHLLNALLTFAYTYWVACMVKFENAKHANTWPIAKGWIYALMFFLLFQITYG
                     AFVAGLKAGLVYNTWPKM"
BASE COUNT         1215 a          700 c          817 g         1319 t
ORIGIN      
        1 cctaagcctg ccggcatttg tgccataaga ttgttagccc atactgataa aataagtagt
       61 aaaatctttt tcatgtttcg atactaaatg taaaatgttt taatggcaat accatttaag
      121 ctacaccatt ttgccacttc gatgtgttgt gtttttaatt gttacaaaaa gtttcttaaa
      181 aaaagtaaat agtcatttgt caaaaacatg taacatatag tagggtaggt ggtctattaa
      241 acattacgtt taccacatgt tattttttaa attatttaag gagagtattt ggtttgcctt
      301 acatgctttg cgtgtaaaca agctgcgcac ttttttatct ttattaggcg taagtgtggg
      361 catatttgcc attatagctg tatttacttt cgtagatgcc atgcaaaaaa gcgtaagcga
      421 tagtataaaa tcattaggcg ataatacatt gtatgtacaa aaatggccat ggacctttac
      481 cgaccctaac tatgcatggt ggaaatactt taagcggcca ctgcccgata ttaaagaagt
      541 agcacaactt aaactacgca ccaccagttt taaggccttg gtttttcagg catcgagcaa
      601 caaccaaatt gttaaattta aaaacaatac agttgaaggc agcagtttat tatttgccga
      661 ctatgatttt aatcaggtta gctcatttga aattagcaat ggacgatact ttacacccat
      721 tgaaagtgat gcaggccggc ctgtgtgtat tatcggatat gaaatagcca aaaacttatt
      781 tccattttca aatccggttg ggcagcaagt acaggcaaaa ggacgtaagc tcaccgttgt
      841 tggtacattt aaaaaagcgg gcttaaatgc tatgggtgaa gattatgata atgtactttt
      901 agcgcctatt aattttggca gtaatatttt agacctaaaa tcagaaatgt tagacccatt
      961 tatctatgca aaaggcattg atggaataag taacgaagcc ttaattgacg agttgcgtgc
     1021 cgctatgcgc aatgtacgca ggttacgccc tactgacgaa gataacttcg ctattaacga
     1081 atcgaaaatg ctgatacaaa gtgtaaccgg tatttttgat gtattgcaac ttacaggatg
     1141 gattattgga ggcttttcaa tattggtagg aggttttggt attgccaata ttttatttgt
     1201 atcggtaaaa gaacgcacca gcttaattgg cattcaaaaa tcgttaggag ctaaaaacta
     1261 ttttatcctg cttcagtttt taatcgaagg tgtggtgcta tgcctgattg gtggtatagt
     1321 tggtttggtt ttggtttacc tatctaccat gggcttagct gctgccgatt ttttaccatt
     1381 cgaagtttca cttaccttaa gcaatattgt tttggcatta acggtatcaa cacaaattgg
     1441 tttgttggca ggttttgtac cggcatacac agcatcgcaa ctcgaccctg ttgaagccat
     1501 cagaagtaat taatcgaata aaatataata aaccatggct acagttttac acaaacgacc
     1561 tttactgcta agcgtagttt gtattgtaaa ttttgtattg ctggtagcag gctttatgtt
     1621 ggtttcttcg ccatttgtac gaaacttagg tgtttggttt ccggcaattt acggcacact
     1681 ggtactaaca aagtttgccg gtactgtggg cgtgtggtat caaaaaaaat ggggtgtatg
     1741 gttgtttgct gcctcctata ttgtaacaca atttgttgat gtatttattg ataatgtatc
     1801 ctggtttggg cttttttatt atgcaggtgc tgccataata tttttttgtt tttacaacaa
     1861 gatggatggc aacttgtaaa ttttaagaag tgaattttta gcaaacaaaa cataaagcat
     1921 ttacttaaaa tttactttcg tgcacatttt taatgctgct tgccttaggg cagtttttgg
     1981 tggctataat gcaatttaca caactgtaaa atgttaaaca tgcaagtata taagtttggt
     2041 ggtgcatcgg ttaaagatgc agatgcgata aagaatgtag cagctattgt atcggcagca
     2101 acacaacctt tggtagtagt agtatcggca atgggcaaaa ccaccaacgg tttagaagct
     2161 gtggttaatg ccggtttcta taaaaatggc gattcaaaaa gtttgctcga tgcagttata
     2221 aaatttcatc aggatatttt gcagcaactg tttgaggcaa cggatgatgt atttacgaag
     2281 gtaaataact tttgggtaga gatagcctgg gcactagaag aggagccacg tagctatggt
     2341 tttttatatg atcagattgt aagtataggc gagttagtag ctactaccat aattgcagca
     2401 tacttaaacc ggcaaggtac tgccacacaa tggttagatg cacgcagctt aatttttacc
     2461 gataataact acaggcaggc tcaaatacaa tggccgcaaa cagttttaca gataaatcaa
     2521 ataatagcac cggtttgtaa tcaaaacaag gttgcgctta cacaaggttt cattggcggc
     2581 accagcgaaa actatacaac aacactgggg cgcgaaggca gcgattatac agcagcaata
     2641 attgcttatt gcttagatgc acaatcggta actatttgga aagatgtgag tggcgtgtta
     2701 aatgccgatc ctaaattttt tagcgatgca caaaagttag agcaacttag ttatcacgat
     2761 gctattgaac ttacttatta cggagcaacg gttattcacc caaaaactat taaaccatta
     2821 gaaaataaag gtattccgct ttatgtaaaa tcatttgtaa agcccgaagg agtaggtact
     2881 gttattggta aagatttaca aaccaaaccc tttataccgt cctatatttt taaaagcaat
     2941 caggtgctaa tcagtattgg gtcgaaagac ttttcattta tagccgaagc cggcctaagc
     3001 cacttgtttg gtttatttgc tgcagcgggc gtaaaaatta atttaatgca aaactcggcc
     3061 atcagctttt cagtatgtgt tgatgatgat gcttttgcat tacctgcatt actcgaaagt
     3121 ttaaaagccg attatcgtgt tttgtttaat aacggtttgg tgctttatac aatcaggcac
     3181 tacaatcaat ttacaataga taaactgctg caaaagggaa cacttatact tgaacaacgc
     3241 tcgcgcaata cggcccaatt aattatgtat ccgaatgccg attaaaaggc aacgcgttac
     3301 tgcgctttaa gtttgtttat tcaaaccttt caatttattt ctatgcaaac caacaatgct
     3361 aaacccgcca taacatcatc gcaacgtgca ttatactatt ggcttttaac aggctgctta
     3421 cttatttttt taatggtagt tattggtggt attacccgtt taaccggtag tggcctttca
     3481 ataaccgatt ggaaaattgt taccggtact ttgcctccat taaccgatag ccaatggcag
     3541 caagagttta tgagctacca gcaaacacca caatataaat tagttaatgc acactttact
     3601 ttaaccgatt ttaaaggcat ttactggtgg gagtatttac atcgtttaat aggcaggtta
     3661 attggtttgg tttttataat accctttata attttctaca aacgcaaatg ggtttccagt
     3721 aaattgttgc ctcatttagt tgttattttt ttaatgggcg gtttccaagg ttttttaggt
     3781 tggtatatgg ttaaaagcgg cttgcttagt cagcccagtg tgagccatat ccgcctggcc
     3841 atgcacttgc taaatgcatt gttaaccttt gcctatacgt attgggttgc ttgcatggtt
     3901 aaatttgaaa acgcaaagca tgctaataca tggcccattg ccaaaggatg gatttacgca
     3961 ctgatgtttt ttttactttt tcagataacc tatggcgctt ttgttgccgg cttaaaggca
     4021 ggtttggttt acaatacctg gcctaagatg g
//
LOCUS       DAOMAA010000043         4028 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_15275_length_4028_cov_3.422603, whole genome shotgun sequence.
ACCESSION   DAOMAA010000043 DAOMAA010000000
VERSION     DAOMAA010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4028
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15275_length_4028_cov_3.422603"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="PLO59_01080"
     CDS             complement(<1..805)
                     /locus_tag="PLO59_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HQU99713.1"
                     /translation="MMLQTKKQLSIFISLLLLIACTVLVVAQSKTNKKDLEKKKEQLL
                     REIDDTNKQLKLNAKNKNATKSQLESLTRKLKARQALINTINTQLTELDGAITQSNGE
                     IIVLQQKMEALKKQYAAMIVYAHKNQNNYQRMMFVFSSNDFNQAYKRIKYMQQYSSFR
                     KQQAATITHTQVALNNKVTDLEAQKVEKSKLKSGQEDQKKTLAQEQSQQELYLKNLSV
                     QETVLRKQLANKQAQKKKLENAIEAAIKKEIEAAKKKAANEGKKNVTKEN"
     gene            complement(908..1792)
                     /locus_tag="PLO59_01085"
     CDS             complement(908..1792)
                     /locus_tag="PLO59_01085"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HQU99714.1"
                     /translation="MRIINLQCCGIKRYQYIFGTITFLAIALTSCKTTKKTTAIAAVP
                     VTDTINTQITAQQLLDSVTKHAFAPSFLSAKANVEANLTDDNKSFNVTLRIKNDSAIW
                     ISINALLGVEAARVLITKDSLKLIDRIHNKFAIADYDYLSDILRIKVDYKTIEQILLG
                     NFFAYRNENRFNSVYTEDNYFILSTLNKRKLRRSLEDKDPNKPIVQDFNIDPATYRIQ
                     TMNVDDDRINKNMHTTYSNFLQLPEGLLPLNSETFITAEQRIGIKINYTKVNTGTAED
                     MPFRIPASFERIVPAPKK"
     gene            complement(1797..3533)
                     /locus_tag="PLO59_01090"
     CDS             complement(1797..3533)
                     /locus_tag="PLO59_01090"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQU99715.1"
                     /translation="MMFKYYKNATLCTCVMLLLAANPIWAQRKKNNSNLSEGEKLAVD
                     KNFLDANREKIMGNDVHALDLFEAVIKVDHKNAASYYEASHLLWKQKKALKAVEYANI
                     AAGLYPDNEWYLMWQAELLQRMGENKQALKVYEQLVEIKPNNIEFYLLKASALFDEKK
                     YTEAIDVYNEVEKRFGVDPTIIREKQRIWLKLGKVDKAAAELEKLIAKQPDDPTYYIL
                     MIELYQANNLYDDVLKTIKRLETAQPNHPAVALSYAEYYRTIHKNDLSFNWLQKAFTN
                     ADLQSELKTKVLMSYIGLAQDNDTMMQQALELSKIFSQTNSAEFIPHAMYGDFLMMDK
                     QLEPAMLQYREALKIEKNNVQIWQQLLQCSAELNNYQTLADDSKEAITFFPDQPLFYY
                     FNGVANGILKKNDEAVQSLNNGAKLVVDNDNLLVEFFSSLGDNYHSLKKYTESDAAYE
                     KALKIDPRNIPVLNNYAYYLSLRKSNLSRAAEMSKLSNDLSPDNGTYQDTYAWVLFQQ
                     GNYAQAKDWQAKAIKNSNPPSGTLFEHYGDILYKLGEVAEALNFWKKAKETGEFSDLL
                     EVKISRSAYVEN"
     gene            complement(3610..>4028)
                     /locus_tag="PLO59_01095"
     CDS             complement(3610..>4028)
                     /locus_tag="PLO59_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HQU99716.1"
                     /translation="QLTNAMENMKNKGLKLLPGKVAEWLDCGNKDATVYTNQRVLELK
                     YPENHIAADATITDSVIIPPCFIGTGATVTQSVIGPHVSIGAQTKVEQSIIKNSIIQT
                     QCNIKQAAIANSMMGNNVLYKGQMADVSLGDYSVNN"
BASE COUNT          999 a          712 c          814 g         1503 t
ORIGIN      
        1 cgttttcttt ggttacattt tttttgcctt cgttggctgc ttttttcttg gctgcttcta
       61 tttctttttt tatggctgct tctattgcgt tttcgagttt tttcttttgt gcttgtttat
      121 tggctaactg tttacgcaat accgtttcct gaaccgataa gttttttaag tataactctt
      181 gttgtgattg ttcctgagcc agggtctttt tttgatcttc ttgtcctgat ttaagttttg
      241 atttttcaac cttttgtgct tctaaatcgg ttactttatt attaagcgct acttgtgtat
      301 gggttatggt tgcagcttgt tgttttctga aactgctata ttgttgcatg tactttatgc
      361 gtttatatgc ctgattaaaa tcgttgcttg aaaaaacaaa catcattcgc tggtagttgt
      421 tctgattttt atgtgcataa actatcatag cagcgtactg tttttttaag gcttccattt
      481 tttgttgcag cactataatc tcgccattgc tttgtgttat ggcaccatct aattcggtaa
      541 gttgtgtgtt gatggtattt attaatgcct gccgcgcttt tagttttcgt gttaatgatt
      601 ccagctggct tttggttgcg tttttgtttt tggcatttaa tttaagctgc ttattagtgt
      661 catcaatttc gcgtaagagt tgttccttct ttttttctaa atctttttta ttggttttgc
      721 tttgtgcaac aaccagtact gtacaagcaa ttaacaacaa aagactaata aaaatggata
      781 actgtttttt tgtttgcaac atcattaatt aaccgaacag ctaaactact tctgtgtatt
      841 actgccttat caaagcaacg ttaaagtaac taacaaatta cctgttaaaa cagttttgca
      901 gcagtggtta ttttttaggt gcaggtacaa tgcgctcgaa actggccggt attctgaaag
      961 gcatatcttc agccgttccg gtgtttactt tggtgtaatt tatttttatg cctatgcgtt
     1021 gttcggccgt aataaaggtt tcgctgttaa gtggcaataa gccttcgggt agttgtaaaa
     1081 agttgctgta tgtggtatgc atatttttgt tgatgcggtc gtcatcaaca ttcatggttt
     1141 gaatgcgata ggtagcaggg tctatattaa agtcttgtac aatgggttta ttggggtctt
     1201 tatcttctaa tgagcgcctg agtttacgtt tgttaagtgt gctaagtata aaatagttgt
     1261 cttcggtgta aacgctgtta aaacggtttt cgttgcggta ggcaaaaaag ttgccaagca
     1321 atatttgctc aatggttttg taatctactt taatgcgtag tatgtctgac aggtaatcgt
     1381 aatcggctat ggcaaattta ttatgtatgc ggtcaataag ctttagcgaa tctttagtaa
     1441 ttaaaaccct tgcggcttca acgccaagca gggcattaat tgaaatccag atggcactgt
     1501 cgtttttaat acgaagggtt acattaaaac ttttattgtc gtcagttaaa ttagcttcta
     1561 cattggcctt tgcacttaaa aagcttggtg caaaagcatg tttggttacc gagtctaata
     1621 attgctgtgc tgttatttgt gtgtttatag tatcagtaac cggtacggct gcaatggctg
     1681 tggttttttt tgtggttttg caggatgtaa gagcgatggc taaaaaagtt atagtcccaa
     1741 aaatgtattg gtatctttta atgccacagc attgcaaatt gattatgcgc atggttttaa
     1801 ttttcaacgt aagcgctgcg actaattttt acttctaaca aatcagaaaa ctcaccggtt
     1861 tcttttgctt ttttccaaaa gtttaaagct tcggctactt cgcctaattt gtacagaata
     1921 tcgccataat gttcgaacaa agtgccactt ggcggattgc tgttttttat ggctttggct
     1981 tgccagtctt tggcttgggc ataattgcct tgctggaata atacccatgc ataggtgtcc
     2041 tgataggtac cattatcggg acttagatca ttgcttagtt tactcatttc ggcagcccgg
     2101 cttaagttgc ttttgcgtaa tgataaatag taagcataat tatttagcac cggtatgttg
     2161 cgtggatcta tttttaaagc tttttcataa gctgcatccg actcggtgta tttttttaac
     2221 gaatgataat tatcgccaag cgaggaaaaa aattctacca ataagttatc gttatcaact
     2281 accagtttgg cgccattgtt taatgattga acagcttcgt catttttttt caaaatgcca
     2341 ttggctacac cgttaaagta ataaaacaac ggttggtcgg ggaaaaaagt aatggcttct
     2401 ttgctgtcgt cagcaagggt ttggtagttg ttaagctcgg cactacattg taatagttgc
     2461 tgccaaatct gcacattgtt tttttcaatt tttaaggcct cgcggtattg cagcatagca
     2521 ggttccaatt gtttatccat cattaaaaaa tcgccataca tggcatgtgg aataaactcg
     2581 gcactgtttg tttgcgaaaa tatttttgac agttccagtg cttgttgcat catggtatcg
     2641 ttatcctgtg ccaagcctat gtatgacatg agcaccttgg tttttaattc agactgcaag
     2701 tcggcattgg tgaaagcttt ttgcaaccag ttaaacgaca ggtcgttttt gtggatagta
     2761 cggtaatact cagcataact aagtgcaact gccggatggt taggttgtgc tgtttctaaa
     2821 cgtttaatgg tttttaaaac atcgtcatat aagttatttg cctgatacaa ttcaatcatc
     2881 aaaatgtagt aggttggatc gtcaggttgt tttgcaatca gtttttcgag ttcggctgcc
     2941 gctttatcta ctttacctaa cttaagccat atacgctgct tttcgcgaat gatagttgga
     3001 tcaacaccaa agcgtttttc cacctcattg taaacgtcaa tggcttcggt atattttttt
     3061 tcgtcaaaca aggcactggc cttcaataaa taaaactcga tgttattggg ttttatttca
     3121 accagttgct catacacttt aagcgcttgt ttgttttcgc ccatacgctg taacagttcg
     3181 gcttgccaca tcaaatacca ttcgttgtcg ggatataaac cggcagcaat gttggcatat
     3241 tctacagcct taagcgcttt tttttgtttc caaagcaaat gcgatgcttc ataataagaa
     3301 gccgcatttt tgtggtcaac ttttataaca gcttcaaaca aatcgagggc atgtacatcg
     3361 ttgcccataa ttttttcgcg gttggcatct aaaaaatttt tatcaactgc taatttttcg
     3421 ccttcgctta agttgctgtt gttttttttg cgttgtgccc aaattgggtt tgctgctaac
     3481 aaaagcatta cacaggtgca aagggttgcg tttttatagt acttaaacat catgtgtaat
     3541 taacgaatat tagcaggcaa tttataggaa gcttcttgtt ttgctttagt taaatgtatg
     3601 tataaacgct tagttattta cactatagtc gcccaaactt acatcggcca tctggccttt
     3661 atacagcacg ttgttgccca tcattgaatt ggcaatggca gcttgtttaa tattgcattg
     3721 tgtttgtata atgctgtttt taataatgct ctgttcgacc ttggtttgtg caccaatgct
     3781 tacgtgtggg ccaattacac tttgggtaac cgttgcaccc gtgccaataa aacagggagg
     3841 aattataacg ctatcggtaa tggtggcatc ggctgcaata tggttttcag gatatttcag
     3901 ttcgagtacg cgttgattgg tatatacggt tgcatcttta ttgccacaat ctaaccactc
     3961 ggcaactttg cctggcaaaa gctttaggcc tttgtttttc atgttttcca tggcattggt
     4021 taactgaa
//
LOCUS       DAOMAA010000044         3999 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_15485_length_3999_cov_2.993661, whole genome shotgun sequence.
ACCESSION   DAOMAA010000044 DAOMAA010000000
VERSION     DAOMAA010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15485_length_3999_cov_2.993661"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..983)
                     /locus_tag="PLO59_01100"
     CDS             complement(<1..983)
                     /locus_tag="PLO59_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009281674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HQU99717.1"
                     /translation="MKKYIIAAFILALSQGAWCQTKLIEKIDRKAGELTIPYSKYVLS
                     NGLTVVIHEDHSDPIVHIDVTYHVGSAREIIGKSGFAHFFEHMMFQGSKHVGDDQHFK
                     MVSEAGGTLNGTTNRDRTNYFETLPKNQLEVGLWLEADRMGFLLDSVTQQKFEIQRST
                     VKNERGQNYDNRPYGLVGETTSRVLYPKGHPYSWLTIGYISHLDAVDVNDLKAFFMEY
                     YGAANATLTVGGDVNTADVLKLAEKYFGSIPAGPKVEKLKMPKVTLNDTRYISGADNI
                     RFPLIQRTYPTVPFYSKDEPALDILASILADSKSSVFYKNFIKTQLGNSAQS"
     gene            1199..1750
                     /locus_tag="PLO59_01105"
     CDS             1199..1750
                     /locus_tag="PLO59_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99718.1"
                     /translation="MAKRIIRNQLAQLIFNRELRQIKRKPQTLSLESVRSIGILYDAT
                     ENENYEVVKNYVRELRELKKEVLALGFVDQKELPNMRFSKLGLDFFTRKNLNWYFKPS
                     HPMVTKFINADFDILIFLNIEQLLPLKYIAAATQAKFKIGKYDAKNAAFCDFMIKTNE
                     QVTLPKFIDLVNNYLKVLSHVQP"
     gene            1737..2615
                     /gene="dapA"
                     /locus_tag="PLO59_01110"
     CDS             1737..2615
                     /gene="dapA"
                     /locus_tag="PLO59_01110"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008505527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HQU99719.1"
                     /translation="MYNLNLAGTGVAIITPFLKNNGVDYAALSNLVKHVVKNGVNYIV
                     VLGTTGESVVLSADEKHAVIKTIHTAMPKKVPLILGVGGNHTTEVVNQLKLVDATLID
                     GILSVTPYYNKPTQNGLYQHYKTIAAATSLPIIIYNVPGRTGVNMTASTQLALANDVK
                     NIVATKEASGNVEQIMSIINQKPKNFSVISGDDVLTLPLMACGAIGVISVSANAYPAE
                     VSKMVNLCLQGKFEAAQKLHYRLMPITQMMFAEGSPAGVKEILQHKNLCTNKVRLPLV
                     NVSKQLSKNFTKYLDV"
     gene            2623..>3999
                     /locus_tag="PLO59_01115"
     CDS             2623..>3999
                     /locus_tag="PLO59_01115"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HQU99720.1"
                     /translation="MPFVRCITYYICFCAVPAIAQQSFISPLDISLKLSGNFGEIRNN
                     HFHSGIDIKTDSATGKNVYAVADGHIARIKVSAVGYGNALYLKHDNGYTSVYGHLLQF
                     ADSVRQYVTAEQNLKNSFEVEVFPDSNLFRVKQGAIIARSGNSGSSGGPHLHFEIRDS
                     LEQIVNPQLFGINVADTIQPVIEALAVYDEMKFTALNQNISYDTAHQAYILNDTLSVT
                     THAAFGIMAYDLCNNSSNKLGIYKMQLHIDTTLIWQSNFDYFSFDKTRYVNAHIDYAE
                     KVLNKNIIERCFKLPGDSFNVYHVYPYKLKAQKIYHAAITLADINGNRNKLIFCFKTN
                     AKTAKPQKPHHSWVWLKYNQPYNLKTNTFLLILPSGSLYQNCYLKYHTSKAGNNYLSA
                     IHHINDNTIPLHKAATISIKTKLDSALQSKALIIQIDSAKTSYIGGLYKNNAVSALIR
                     TFGSFAVVV"
BASE COUNT         1302 a          715 c          738 g         1244 t
ORIGIN      
        1 gattgtgccg agttgcctaa ttgtgttttt ataaagtttt tataaaaaac gctgcttttt
       61 gaatcggcaa gtatgctggc caaaatatct aaagcgggtt cgtctttact ataaaatggc
      121 acggttggat acgtacgttg aattaatgga aatcggatat tatctgcacc ggaaatatat
      181 cgtgtatcgt ttaatgtaac ttttggcatc tttagttttt caactttagg gccggcaggt
      241 atgctgccaa aatatttttc ggccagcttt aaaacatcag ctgtatttac atcgccacca
      301 acggttaagg tagcattagc tgcaccatag tattccataa aaaatgcttt taagtcgttt
      361 acatctacag catctaaatg tgatatataa ccgatggtta accaactgta tggatgcccc
      421 tttggataca aaacacgtga tgtggtttcg ccaaccaaac cataaggccg gttatcgtag
      481 ttttgaccgc gttcattttt aacggtgctt cgttgtattt caaatttttg ctgtgtaacc
      541 gaatccagta aaaaacccat ccgatcagct tcgagccata aacccacttc caactgattt
      601 ttaggcaacg tctcaaaata attggtacgg tcgcggttgg tagtaccatt tagcgtgccg
      661 cctgcttcgc tcaccatttt aaaatgttga tcatcgccaa cgtgcttact gccttgaaac
      721 atcatgtgtt cgaaaaaatg cgcaaagccg cttttgccga taatttcgcg tgcactgcct
      781 acgtggtagg ttacatcaat atgtacaata gggtcgctgt ggtcttcgtg tataaccacc
      841 gttaggccat tgcttaaaac atatttacta tagggtatgg ttaactcacc ggccttacgg
      901 tcaatttttt cgataagttt tgtttgacac caggcacctt ggctcaaagc caaaataaaa
      961 gctgcaataa tgtatttctt cattaaaatt agggtttaat atttgggaca aattaaacaa
     1021 aacctaaaca cttcaggggg tatcatttaa aaacaaagta tctatccgca aaaaattggc
     1081 attataaaat gaagttgtat ttgcaaattt aaaaaatggc ctacttttgt tacagttatc
     1141 atttttatgt actgtattta tatacctttt tcactattgt aagtttcggc aacaggtaat
     1201 ggcaaaacgc atcatccgta atcaactggc acaactaatt tttaatcgtg aattgcggca
     1261 aattaagcgc aaaccacaaa ccctttcgct agaatcggta cgtagtattg gcattttgta
     1321 tgatgcaaca gaaaacgaaa attatgaagt ggtaaaaaat tacgtgcgcg aattgcgtga
     1381 actgaaaaaa gaagtactgg ctttgggatt tgttgatcag aaagaactgc ccaacatgcg
     1441 tttttcgaaa ttaggtttgg atttttttac gcgcaaaaat ctgaactggt attttaaacc
     1501 atcacatccg atggttacca agtttattaa tgccgatttc gacattctta tctttttaaa
     1561 cattgaacag ttattgccgt taaaatatat agctgcagca acacaggcta aatttaaaat
     1621 aggaaagtat gacgctaaaa atgctgcatt ttgtgatttt atgattaaaa ccaatgagca
     1681 ggttacctta cccaaattta ttgatttagt aaacaattat ttaaaagtat taagccatgt
     1741 acaaccttaa tcttgccggt accggtgttg caataattac tcctttttta aaaaataatg
     1801 gtgttgatta tgcagcctta tcaaacctgg taaagcacgt agtaaaaaat ggcgttaatt
     1861 atattgtagt gttgggtaca acaggcgaaa gtgtagtgct tagtgctgac gaaaagcatg
     1921 cagttattaa aaccattcat actgccatgc ccaaaaaagt accgctcatt ttaggtgtgg
     1981 gcggtaacca taccaccgaa gtggttaacc agttaaagtt ggttgatgct actttaattg
     2041 atggcatttt aagcgttacg ccttattata acaaacccac acaaaacggc ttgtatcaac
     2101 attataaaac cattgctgca gctacatcat tgcccattat tatttacaat gtacccggcc
     2161 gtaccggagt taatatgaca gcaagtacgc aattggcttt ggcaaacgat gtaaaaaata
     2221 tagttgctac taaagaagca tcgggtaatg tggaacaaat catgtctatt ataaatcaaa
     2281 agcccaaaaa ttttagtgta ataagtggtg atgatgtact tacattacca ctaatggcat
     2341 gcggtgccat aggtgttatt agcgttagtg ccaatgctta tccggccgag gtcagcaaaa
     2401 tggtaaacct ttgtttacaa ggcaagtttg aagccgcaca aaaattacat tatcgtttaa
     2461 tgcccataac acaaatgatg tttgccgaag gtagtccggc cggagttaaa gaaattttac
     2521 aacacaaaaa cctttgcacc aataaagtgc ggttgccatt agtaaatgtg agtaagcaac
     2581 taagcaaaaa ttttactaag tacctggatg tttaaattcc tgatgccttt tgtaaggtgt
     2641 atcacttact atatatgttt ttgtgctgtg ccggcaattg cacagcaatc atttatcagt
     2701 ccgttagata ttagcttaaa gttatcaggc aactttggcg aaatcagaaa caatcacttc
     2761 cattcaggta ttgatatcaa aaccgatagt gcaacaggta aaaatgttta tgcagtggcc
     2821 gatggtcata tagcccgcat taaagtgagt gctgtaggtt acggcaatgc cttgtatttg
     2881 aaacatgata atggatatac atcggtttat ggccacttat tacaatttgc cgatagtgtc
     2941 aggcaatatg ttacagcaga acaaaattta aaaaacagtt tcgaagttga agtgtttcct
     3001 gatagcaact tgtttcgggt taaacaaggg gccattatag cccggagcgg aaattcggga
     3061 agttcgggtg gcccgcattt gcattttgaa atacgtgata gtttggagca aatagtaaat
     3121 ccacaattat tcggaataaa cgttgccgat acaatacaac ctgttattga agctttggcc
     3181 gtatatgatg aaatgaaatt tactgcctta aatcaaaata ttagttatga tacggcacat
     3241 caagcttata ttttaaatga tactttatcg gtaactaccc atgctgcatt tggtattatg
     3301 gcttatgatt tatgcaataa cagcagtaac aaattgggta tttataaaat gcaattgcat
     3361 atagatacta cattaatttg gcaaagcaat tttgattatt tttcatttga taaaacgcgt
     3421 tatgtaaatg cacatattga ctatgccgaa aaagtgctga ataaaaacat tatcgaaaga
     3481 tgttttaaac tacctggcga ttcgtttaat gtttaccatg tttatccgta caaattaaaa
     3541 gcacaaaaaa tatatcatgc agcaattaca ctggctgata taaatggcaa tcgaaataaa
     3601 ttgatttttt gttttaagac caatgcaaaa actgctaagc ctcaaaagcc acatcatagc
     3661 tgggtttggc ttaaatataa tcaaccttac aatctaaaaa caaatacttt tttattgata
     3721 cttccttcgg gtagcctgta tcaaaattgc tacctaaagt atcacacatc aaaagccggc
     3781 aacaattatt tatcggccat acatcatatt aatgataaca ccataccatt acataaagcg
     3841 gcaaccattt cgataaagac aaaattagat agcgctttac aaagtaaggc tttaattatt
     3901 caaatagata gtgcaaaaac cagctacatt ggtggtttat ataaaaacaa tgcagtaagt
     3961 gctttaatcc gaacctttgg cagttttgct gttgttgtt
//
LOCUS       DAOMAA010000045         3930 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_15986_length_3930_cov_3.224258, whole genome shotgun sequence.
ACCESSION   DAOMAA010000045 DAOMAA010000000
VERSION     DAOMAA010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3930
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15986_length_3930_cov_3.224258"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="PLO59_01120"
     CDS             complement(<1..292)
                     /locus_tag="PLO59_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99721.1"
                     /translation="MKIFKLIVLKMFCIVLVQAQTKQPKIKVYLLGTFHFAQTDSTYD
                     VLETKHQQSIAKLCKIVEKQNPDKIFIERMPDFEYVNRMDSLFRAFKNVDTVV"
     gene            complement(300..1178)
                     /locus_tag="PLO59_01125"
     CDS             complement(300..1178)
                     /locus_tag="PLO59_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQU99722.1"
                     /translation="MLHINEIEKTYGNGVKALNKITLQIGKGMFGLLGPNGAGKSTLM
                     RTIATLQLPDKGSIYFENTNVLANPQSLRYCLGYLPQDFGVYPNVSAQSLLNYFAVLK
                     GIVSKKQRSQIIQEVLEITNLSNHRAENVDGFSGGMKQRFGIAQLLLNKPKLIIVDEP
                     TAGLDPNERNRFLNVLREIAIDNTILFSTHIVDDINDLCNKIAIINKGCLQLHTEPLK
                     AVNQIRGYIWQKLINRNELEQVVQRFKVLSVKYQSNQLTVRVYATNQPDMDFVLAEPN
                     LEDVYFNVMYQSNNQH"
     gene            1503..>2020
                     /locus_tag="PLO59_01130"
     CDS             1503..>2020
                     /locus_tag="PLO59_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015808785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HQU99723.1"
                     /translation="MATIRFKALETASARTSRTVKPPSEKISDYFGTHVFSRNTMQKT
                     LSKEIYNHMVAAIDSGHQIDRKTAEQVAAAMRTWAMSKGCTHYTHWFQPLTGTTAEKH
                     DAFFDLSDGSPIETFSSNALVQQEPDASSFPNGGIRNTFEARGYTAWDPSSPAFIMES
                     TAGKTLCIPTIFV"
     assembly_gap    2021..2030
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2031..3705
                     /locus_tag="PLO59_01135"
     CDS             <2031..3705
                     /locus_tag="PLO59_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015808785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine synthetase type III"
                     /protein_id="HQU99724.1"
                     /translation="SYTGETLDYKAPLLKALHAIDKAAVDVCQYFDKNVTKVQPTLGI
                     EQEYFLIDTALYQARPDLMMTGRTLLGHAAAKGQQLEDHYFGSIPSRVYAFMVEFEIE
                     AFKLGIPLKTRHNEVAPSQFECAPIFEEINLAVDHNQLLMDLMDKIAARHHFKVLLHE
                     KPYAGINGSGKHNNWSLGTNTGKNLLSPGSTPKSNLQFLTFFINTIKAVHDHADLLRA
                     SIASASNDHRLGANEAPPAIMSIFLGQQLSNVLDDLEKKVRTKDKMTPEQKTDLKLNI
                     AKIPEILLDNTDRNRTSPFAFTGNKFEFRAVGSSANSSHAMMVLNTIVANQLTIFKAE
                     VDKLIEKGQGKDEAIFKVLHDYIVDSKKIRFEGNGYGDEWKREAKKRGLSNVTTTPPA
                     LDALVSKTSLALFEKMQIFTHREAEARHEILLEDYTKKIQIESRVIGDLSLNHIIPTT
                     LRYQTMLIENVEGLHDIGLKGIYSKTQIELIKEISERVTRIKTNVDAMVEARKKANVI
                     ENARTRAIAYCDKVKPFMDIIRDDVDHLELMVDDEVWPLPKYREITNVR"
BASE COUNT         1326 a          747 c          692 g         1155 t
ORIGIN      
        1 taacaactgt atctacattt ttaaaagcac ggaacaagct atccattcgg ttaacgtatt
       61 cgaaatcggg catccgttca ataaatatct tgtccgggtt ttgcttttca acaatcttac
      121 aaagcttagc aatgctttgt tgatgttttg tttctaacac atcataagtt gagtcggttt
      181 gcgcaaaatg aaaagtacct aacagatata ctttaatttt tggttgctta gtttgtgcct
      241 gtaccagcac gatacaaaac atttttaata caataagttt aaatattttc ataactgatt
      301 tagtgttgat tatttgattg gtacattaca ttaaaataaa cgtcttctaa attgggttca
      361 gccaaaacaa aatccatatc gggctgattg gttgcatata ctctaactgt taattgattg
      421 ctttgatatt ttaccgacag cactttaaac cgttgcacca cttgctctaa ctcatttctg
      481 ttaatcagct tttgccaaat atagcctctg atttgattta cagctttcaa aggttcagta
      541 tgcaattgca aacaaccttt attaataatg gctatcttat tacataagtc attaatatca
      601 tcaacaatat gtgtcgaaaa aagtatggta ttatcaatag caatttctct taaaacattt
      661 aaaaaccggt ttctttcgtt tgggtctaaa cctgctgttg gttcatctac aataatcagt
      721 ttgggtttat taagtaaaag ttgggcaatg ccaaatcttt gtttcatacc acctgaaaag
      781 ccatctacgt tttcagctct gtgattagat aaattggtaa tttctaaaac ttcttgaatt
      841 atttgactgc gttgtttttt ggaaacgata ccttttaaaa cagcaaaata gtttagtaaa
      901 ctttgtgccg aaacattggg ataaacacca aaatcttgcg gcaaataacc caaacaataa
      961 cgcaagcttt gcggattagc aagcacattt gtgttttcga aataaatgct gcctttatcg
     1021 ggcaattgca atgtggcaat ggtacgcatg agcgtagatt taccggcacc attaggtcct
     1081 aacaaaccaa acataccttt accaatttgc agcgtaattt tatttaaggc ctttacacca
     1141 ttgccatacg ttttttctat ttcgtttata tgtaacacgc gccactaaca ttattaagat
     1201 tataaaccga agtagctgtg ttataaataa tttcaaaaaa atttggtgta aggccggatt
     1261 attttgacgt aaaaccctta gaactggtga tgaaattacg cagcatttat ggtaagcacc
     1321 tactgacaaa aaatagtgat gtaaataaac ttacaagcta ccagacagta atttatccta
     1381 ataaaataac aacattgtgc acaaataaag ggggtgttta cattttttta tacttttttt
     1441 tagcataccc ccctattttt tcattgcttt gccgaacttt ttaataaaaa accaaatatt
     1501 caatggcaac cattcgattt aaagcgttag aaaccgcaag tgcacgcaca tcgcgcactg
     1561 taaaaccacc ttcagaaaaa atttctgatt atttcggcac gcatgtgttt agccgaaata
     1621 ccatgcaaaa aacactatcg aaagaaattt ataaccacat ggttgcagcc attgacagtg
     1681 gccatcaaat tgaccgcaaa acagccgaac aagttgcagc ggccatgcgc acctgggcta
     1741 tgagcaaagg gtgtacgcat tatacacatt ggtttcaacc cttaaccgga accactgccg
     1801 aaaagcacga tgcatttttt gatttaagtg atggttcgcc aatcgaaacc tttagcagca
     1861 atgcattggt acaacaagaa cccgatgcaa gcagctttcc aaatggcggt attcgcaata
     1921 cttttgaagc acgcggctat acagcctggg atccatcgtc accggcattt attatggaaa
     1981 gtacagcagg caaaacgctt tgcataccaa ccatttttgt nnnnnnnnnn atcttataca
     2041 ggcgaaacac ttgattataa agcacccctt ttaaaagcac tgcatgccat tgataaagca
     2101 gccgttgatg tatgccaata ttttgataaa aacgtaacca aggtgcaacc tacattgggt
     2161 attgagcagg aatacttttt aattgataca gcactgtatc aggccagacc cgatttgatg
     2221 atgacaggcc gaaccttgtt aggccatgca gcggccaaag ggcaacaatt agaagaccat
     2281 tactttggca gcataccatc acgtgtatat gcatttatgg ttgagtttga aattgaagct
     2341 tttaaattag gcattccgct aaaaacacgt cataacgaag tagcacccag ccagtttgaa
     2401 tgtgcgccca tttttgaaga aattaattta gcagttgatc ataatcaatt gttaatggac
     2461 ttgatggata aaattgcagc aagacatcac tttaaagtat tgttacatga aaaaccttat
     2521 gcaggtatta atggcagtgg caaacacaat aactggagtt taggtaccaa taccggcaaa
     2581 aatttattaa gcccgggctc aacacctaaa agtaatttac aatttcttac cttcttcatc
     2641 aacaccataa aagccgtaca cgaccatgcc gatttattgc gtgcaagtat agcctcagcc
     2701 agtaacgacc accgtttagg cgctaacgaa gcaccaccgg caataatgag catattttta
     2761 ggacagcaat tatcaaacgt gcttgacgac ttagagaaaa aagtgcgtac aaaagataaa
     2821 atgacgcctg aacaaaagac cgatcttaaa ttaaacattg ccaaaatacc tgaaatactt
     2881 ttagataaca ccgaccgtaa ccgtacatcg ccatttgcat ttacaggaaa taaatttgaa
     2941 ttcagagcgg taggttcatc agcaaattca tcgcatgcaa tgatggtgct taacaccatt
     3001 gtggccaatc agttaaccat ttttaaagct gaggtggata aattaattga aaaaggccag
     3061 ggtaaagatg aagccatatt taaggtactg cacgattata ttgttgattc gaaaaaaatc
     3121 agattcgaag gcaatggtta tggcgatgag tggaaacgtg aagcaaaaaa acgcggcttg
     3181 agtaatgtta ccactacgcc accagcatta gatgctttgg taagtaaaac ttcattggca
     3241 ttatttgaaa agatgcaaat atttacacat cgcgaagccg aagcccgtca tgaaatattg
     3301 ttagaagatt acactaaaaa aattcagata gaaagtcgtg taattggcga tttatcatta
     3361 aaccatatta taccaactac actgcgctat caaaccatgc taatagaaaa cgtagaaggc
     3421 ttgcatgata tcggactgaa aggtatttac tcaaaaacac aaatcgaatt aattaaagag
     3481 attagtgaac gtgtaacacg cattaaaacc aatgttgatg ccatggttga agcacgtaaa
     3541 aaagcaaatg ttatcgaaaa tgcgcgcaca cgtgctattg cttactgtga taaagtaaaa
     3601 cctttcatgg acataattcg tgatgatgtg gatcatttag aactgatggt tgacgatgaa
     3661 gtatggccat taccaaagta cagggaaatt accaacgtta gatagttgcc tccaattaat
     3721 tactacgtaa acaattggta ataggtaact gcgctattta aattcgttcc ttaaaataaa
     3781 ataaaaaagc tgccccattt atatgaggca gctttttatt ttcaataggt taccctaata
     3841 aattatcaat ctatcatgcc actgagtatg catgctgctg taccttatgg aataaacacc
     3901 tttggctagc aaatgcaaat tcaattcaaa
//
LOCUS       DAOMAA010000046         3906 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_16140_length_3906_cov_3.078162, whole genome shotgun sequence.
ACCESSION   DAOMAA010000046 DAOMAA010000000
VERSION     DAOMAA010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3906
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16140_length_3906_cov_3.078162"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2395
                     /locus_tag="PLO59_01140"
     CDS             <1..2395
                     /locus_tag="PLO59_01140"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU99725.1"
                     /translation="MDSLTNIATPNALGYINWSVGATWILGNNGTFIKTRASTNAFYQ
                     GNFAGGISNVPNTANWILRKTATQNPTLITANAYYPNLIFENQAGGIYITNGVGTFFS
                     GNSACNVLGNLEIRSAGINAIIVYDSILTNALIVRGDLIINASGSLVLVGDSIRLEGD
                     LNVNGSLIYSTTSPYARQLNFAGSNNQQVGAGTGLIELYNTAIDKSANNLTLFQPVYL
                     FNRLYFSNGLINTTTANTLEFTQAAQAIGFNNNSFINGPCNKWGNAAFTFPIGKLANL
                     QPLAINASASANPFWTENFNNGCTTGCLASSYSSGNGSWTEVDFSPPGACGTSVTNMW
                     YVSCAENGNAVGTCGTGCGNNATMHVGSQSPSPVLSALCPGGDCGAAYDPGGLCPLLG
                     PPFPSTETDIQMESPLIDCSAQSNITLSFNYIAGGDANDTASLWFYDGATWYWITKFT
                     PLTCGTGQGQWRNYTVAVPNYADYNSNFQIAFRWSNNDDGAGQALSFAVDDIQLTALD
                     IFQSEYFYANPQLVYGTNYETGIHHISACEYWHLNRLNGLGNRQVTLTWDANSCGVTS
                     MPDLRVVRFDNGLTQWRNHGNASTTGALAAGTITTSLISSTFGPYTLASVNGLNPLPV
                     EWLNFNAKKIENHVAINWQALIKPLHQYFMVQRSSDLKSIETISEVHDLQTAGNYVSY
                     SVTDTQPMEQNYYRIVQIDFNGEQAFTEWKKVYFESEKFTAWAYNGNLYFTIEPCCSG
                     QKLQVALIDMFGHQVYANTVKASTANVVRLPDLVKGIYILKIEDGHALWQQKLLLGE"
     gene            complement(2411..3421)
                     /locus_tag="PLO59_01145"
     CDS             complement(2411..3421)
                     /locus_tag="PLO59_01145"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HQU99726.1"
                     /translation="MKKIKPKVVQAATLIVIIFLGLFILYALKGFLESFLGAVVVYVL
                     FRNIMLNLCQVRKWSRSSAAILIILATFLIVLVPLVIVTTLFIPKLNMFFDNASYLIH
                     TIENIDQTIKSFVGFEILSDENLKAIQTHAATAVSAVLSSTVNVFGYIAIMYFILYFM
                     LIHVGEIEDAIAKYVPLRSENIARFGKELEAQTYSNAIGSPVLAIIQGLMASLGYWIF
                     GLTEPLFWGIMTGFFSFVPFVGSAIIWLPAAIYLFGIGHTWQAIATVLYGFIVIGSID
                     NIFRFVFQKKFADVPPLITVFGVIVGFNLFGLVGLVFGPLMLSWFVILVEIYYDEYLS
                     NE"
     gene            3520..3735
                     /locus_tag="PLO59_01150"
     CDS             3520..3735
                     /locus_tag="PLO59_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HQU99727.1"
                     /translation="MENSKSNLLVGLVIGAAVGAAIGYFLASDNKEKIIEELKDTANN
                     LKKDFDTHFEKGKKAVDDFITGSEQQV"
BASE COUNT         1296 a          708 c          740 g         1162 t
ORIGIN      
        1 tatggactca ttaaccaata ttgctacacc caatgctttg ggttatatta attggagtgt
       61 cggtgctacc tggatattgg gcaacaatgg aacttttatt aaaacacggg caagtacaaa
      121 tgctttttat caaggtaatt ttgccggtgg tattagtaat gtgcctaata ccgctaattg
      181 gatacttcgt aaaactgcaa ctcaaaatcc aacacttata acagccaatg cctattatcc
      241 aaatttaatt ttcgaaaatc aggctggcgg tatatatata acaaatggcg tgggcacttt
      301 tttttcgggc aatagtgcat gtaatgtttt aggtaattta gaaattaggt cggcaggcat
      361 taatgccatt attgtttacg atagtatttt aaccaatgct ttgattgtaa ggggcgattt
      421 aataattaat gcttcgggca gcttggtatt ggttggagat agtatcaggc ttgaaggcga
      481 tttaaatgtt aatggcagtt tgatttattc tactacgtca ccctatgcac gccaattaaa
      541 ttttgctggt agcaacaatc aacaagtagg tgcaggcacc ggtttaatag aactgtacaa
      601 tacagccatt gataaaagtg caaataacct aacactgttt caaccagttt atttgtttaa
      661 cagactttat ttttctaatg gcttaataaa caccaccact gccaataccc ttgagtttac
      721 acaagctgcg caagccatcg gctttaacaa caatagtttt attaatgggc catgtaataa
      781 atggggtaat gcagctttta cttttccaat tggcaaactg gctaacttgc aaccattggc
      841 aataaatgca tcggcatcgg ctaatccttt ttggactgaa aattttaata acggatgtac
      901 aaccggatgc ttagcaagca gttattcgag cggaaatgga agttggacag aagttgattt
      961 ttcgccacca ggtgcttgcg gtacctctgt aaccaatatg tggtatgtga gctgtgccga
     1021 aaatggcaat gccgtaggaa cttgtggtac aggttgtgga aataatgcaa ccatgcatgt
     1081 aggcagtcag tcaccttcgc ctgtactgtc tgctttatgt cctggcggag actgtggtgc
     1141 tgcttatgat ccgggtggct tatgcccttt attggggccg ccatttccat caacagaaac
     1201 cgatatacaa atggagtcgc ctttaataga ttgcagtgct caaagtaata taacactttc
     1261 gtttaattat atagccggtg gcgatgctaa cgatactgct tcactttggt tttatgatgg
     1321 ggctacctgg tattggatta ccaaatttac acccttaacc tgcggaaccg gccaagggca
     1381 atggcgaaac tatacagttg ccgttcccaa ctatgccgac tacaattcaa actttcagat
     1441 tgcattcaga tggagcaata atgatgatgg tgccggccaa gcgctttcat ttgctgttga
     1501 tgatattcag ctaacagcct tagatatttt tcagtcggag tatttttatg caaatccgca
     1561 attggtttat ggcaccaatt acgaaacagg catacatcac atcagtgctt gcgagtattg
     1621 gcatttaaac cggcttaacg gcttaggtaa ccgccaggtt acattaacat gggacgcaaa
     1681 tagttgtggg gtaacctcta tgcctgattt gagggtagta cgttttgata atgggctaac
     1741 gcaatggcgc aatcatggta atgcaagtac aacaggggcc ttagctgcag gtaccattac
     1801 cacttcgtta atatcaagta cgtttggccc ttatacattg gcttcggtta atggcttaaa
     1861 tccgttaccc gttgagtggt taaattttaa tgcgaaaaaa attgaaaacc atgttgctat
     1921 taattggcaa gcacttataa aaccattgca tcagtatttt atggtacaac gcagtagcga
     1981 tttaaaatca atcgaaacca ttagcgaagt tcacgatttg caaactgccg gtaattatgt
     2041 aagttatagc gtaactgata cgcaacctat ggagcaaaat tattaccgta ttgtacaaat
     2101 tgattttaat ggagagcaag catttacaga gtggaaaaaa gtttatttcg aatccgaaaa
     2161 gtttacagcc tgggcctaca atggaaattt atattttact attgagccct gttgttcagg
     2221 tcaaaaatta caagttgcct taattgatat gtttgggcat caggtttatg ccaatactgt
     2281 aaaagcaagt acagcaaacg tggtcaggct acctgatttg gttaagggta tttatatttt
     2341 aaaaatagaa gatggccatg cattatggca acaaaaacta ttattaggcg agtagtttta
     2401 tttcaggagg ttattcattg ctcaaatact catcgtaata tatttcgact aaaattacaa
     2461 accacgatag catcaaaggg ccaaaaacta atcccaccaa accaaataaa ttaaaaccta
     2521 caattacacc aaataccgta attaatggag gcacatcggc aaatttcttt tgaaacacaa
     2581 aacgaaaaat attgtcgata cttccaataa caataaagcc atataatacg gttgcaattg
     2641 cttgccatgt atggccaatg ccaaaaagat aaatggcagc aggcaaccaa attatagccg
     2701 agccaacaaa aggcacaaat gaaaagaagc cagtcataat gccccaaaaa agcggttcgg
     2761 ttaatccaaa aatccaataa cctaaacttg ccattaagcc ctgtataatt gctaaaacag
     2821 gcgagcctat tgcattacta taagtttgtg cttctaactc ttttccaaac ctggcaatgt
     2881 tttcgctgcg cagtggtaca tatttagcaa tagcatcttc aatttcgcca acatgtatca
     2941 acataaaata taatataaaa tacataatag ctatatagcc aaacacatta actgtcgaac
     3001 ttaatactgc acttactgct gttgctgcat gcgtttgaat ggcttttaaa ttttcatcac
     3061 ttaaaatttc gaaaccaaca aaagacttaa tcgtttggtc aatgttttca atagtatgta
     3121 tcaggtatga tgcattatca aagaacatgt taagcttagg aataaacaaa gttgttacaa
     3181 taaccaaagg caccagtaca attaaaaatg ttgccagaat aatgagtata gcagcactac
     3241 tccgcgacca tttgcgcacc tgacataaat taagcattat gtttctgaaa agtacataca
     3301 ccaccacagc tcctaaaaag gattctaaaa aaccttttaa cgcatataaa ataaagagac
     3361 ctaaaaaaat aattacgatt aaagtggctg cttgtacaac ctttggttta attttcttca
     3421 tagaataaac ggtattatac tcttaaaaaa cttaaaattg cagggttgtt tttggtgtaa
     3481 caaatgaaat aacatagttt taaaacccaa ccaaaaaata tggaaaattc gaaatcaaat
     3541 ttattagtag gattagtaat aggcgcggcc gtaggtgctg ctatcggtta ttttttagcc
     3601 agcgataaca aagagaaaat aattgaagag ttaaaagata cggctaacaa tttgaaaaag
     3661 gactttgata cgcattttga aaaaggaaaa aaagcagtgg atgattttat aactggtagc
     3721 gaacaacaag tttagttatt gcatactaca caggtaaatg ttatggagca aaaaaatttt
     3781 ttagcagccc tcaaatcaga tattataaaa tatgttgatt taaaatttca atacataaaa
     3841 cttagcagtt ttgaaaaaac agcgcgtata gcttctgctg ctaccagtta tttaattatt
     3901 ggcttt
//
LOCUS       DAOMAA010000047         3890 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_16267_length_3890_cov_2.726467, whole genome shotgun sequence.
ACCESSION   DAOMAA010000047 DAOMAA010000000
VERSION     DAOMAA010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3890
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16267_length_3890_cov_2.726467"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1180)
                     /locus_tag="PLO59_01155"
     CDS             complement(<1..1180)
                     /locus_tag="PLO59_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQU99728.1"
                     /translation="MNSGFFKVKFNPINKCVFAGLLLLIIAGCSTKKNTFVSPSYHNL
                     AGHYNGFFNARERVRETSKRLALMQKDQFEKTLPIFKIGDDAQAKSVFPDMDEAIKKS
                     SIVIQRHSMNIKGKEYCKWIADNYLVIGKAQFYKHDFWAASETFQFIATEYKTDPLRF
                     EAMIWLTQVYLQLGKTVDAEYVIDYLKNEPGMPRQYRGLFYTVAADFYLKQRDELNAI
                     ECLEKTLTTKLKRDQKIRHMFILGQLYQRNGQSDKAYRIYEQVIKMNPTYEMAFNAKI
                     NRARNFDANNASATEVKKQLQKMVKDDKNIEYLDQIYYALAGVANKENDEVAEIDYLN
                     KSVRASISNANQKALSYLNLGEIYFSKKDFIYAQAYYDSCLQNLTKDYPDYTIIEDKK
                     L"
     gene            1329..2195
                     /locus_tag="PLO59_01160"
     CDS             1329..2195
                     /locus_tag="PLO59_01160"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HQU99729.1"
                     /translation="MSNSFRKYLASRMRTKLRLVIMKDETFEEKFALRLTPLKTFILV
                     GVSLISLIILTTYIIAFTNLREYIPGYADVGMKRNLIYLSLKTDSLTQKIIAQEQYIS
                     NLNEVINGKAGAYQGVDTNAKALYSDSLSLPKSAADSQLRQLIESQDQYDLDFISQQK
                     HATGIAGFSYFTPVTGTITETFNPALKHFGIDVVAAPNEPVKSTLDGTVVFAGFTSAT
                     GYVIAVQHSGNLFSIYKHNAGLLKSVGDYVNAGDAIAIIGNSGELSQGPHLHFELWYN
                     GSAVNPTDYMSF"
     gene            2310..3008
                     /locus_tag="PLO59_01165"
     CDS             2310..3008
                     /locus_tag="PLO59_01165"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HQU99730.1"
                     /translation="MAHATPTNDSKFTTNLFLIYMLSTAVLVSALIYCVGATHTIDHA
                     SNPHTGLKAPAVGHGGHEAHDSHAADTKTEPHAAETHAAVKHELPGGLHIEVAAGSIE
                     DKLIAFIQDPHAQVSKDLWFDFNDLLFKTNEAVLEPQSMQQLKNVAAIFKAFPAVKAK
                     IGGYTDNTGDAQANLKLSDARAKSVMQTLVTEGIAADRLEAEGYGQEHAVASNDTEEG
                     RALNRRISLSVRAK"
     gene            3097..>3890
                     /gene="dxr"
                     /locus_tag="PLO59_01170"
     CDS             3097..>3890
                     /gene="dxr"
                     /locus_tag="PLO59_01170"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019946012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HQU99731.1"
                     /translation="MQRRVAILGSTGSIGKQALEVISMHSQSFIAEVLTAQSNAETLI
                     NQALTFNPNVVVIGDANKYETVKQALAHTDVKVYAGADAIAQVVTMDTIDIVLTALVG
                     YAGLLPTIAAIKAGKPIALANKETLVVAGEIITQLAAQHKVVLLPVDSEHSAIFQCLQ
                     GEWDNAIEKIYLTASGGPFRGKDLKYLVSVTKAQALKHPNWSMGAKITIDSASMMNKG
                     LEMIEAKWLFNLSETQIDVVVHPQSIIHSMVQFEDGSIKAQMGLPD"
BASE COUNT         1196 a          782 c          759 g         1153 t
ORIGIN      
        1 ctaacttttt atcttcgatt atggtataat cgggataatc tttggttagg ttttgcaagc
       61 agctatcgta gtatgcttgc gcataaataa agtctttctt gctaaaataa atttcgccta
      121 agtttaagta tgaaagtgct ttttgattgg catttgatat actggcccgt accgatttgt
      181 tgaggtaatc aatttcggct acttcatcgt tttccttatt agctacacct gccaaagcat
      241 aataaatctg gtctaagtat tcgatgttct tatcgtcttt taccattttt tgcaactgct
      301 tttttacttc ggtggcgctg gcattatttg catcaaaatt gcgggcacga ttaatttttg
      361 cattgaaagc catttcataa gtcgggttca tttttattac ctgctcatat atgcggtagg
      421 ctttatccga ctggccatta cgttggtaaa gctgacctaa aataaacatg tgccgtattt
      481 tttggtcgcg ttttaatttg gtggttaatg ttttttccaa acattcgatg gcattaagtt
      541 catctctttg tttgagataa aaatcggcag caacagtgta aaataaacca cggtattggc
      601 gtggcatacc gggttcgttt ttaagatagt caattacata ttcggcatca acagttttgc
      661 ccaattgtaa ataaacctgg gttaaccata tcatagcttc gaaacgcaac gggtcggttt
      721 tatactcggt ggctataaac tgaaacgttt cggatgcagc ccaaaaatca tgtttataaa
      781 actgtgcttt gcctattact aaataattat cggcaatcca tttacaatac tctttacctt
      841 taatattcat gctatggcgc tgaataacga tagagctttt tttaatggct tcgtccatat
      901 ccggaaatac ggatttggcc tgtgcatcgt caccaatttt aaatatgggc aatgtttttt
      961 cgaactgatc tttttgcatc aaagccaaac gctttgaagt ttcgcgtaca cgctcgcgtg
     1021 cattaaaaaa accattgtaa tggcctgcta aattgtgata gctaggcgat acgaatgtgt
     1081 ttttttttgt actgcaacct gctattatca acaatagcaa gcctgcaaat acacacttgt
     1141 ttatggggtt aaatttcact ttaaaaaaac ctgaattcac tttaactgta aaagcctaat
     1201 tttattgcag ttggcaaata tataaagtta aacaagtttg cttacttgtt tgcaaaagca
     1261 tctgcatgtt aggcccattt taatttacat atttgcggcc attataacgg tgccgcaaat
     1321 ataacatcat gtcaaactca tttcgtaaat acttagccag ccgtatgcgc accaagctta
     1381 ggctggttat aatgaaggac gaaacctttg aagaaaagtt tgcactacgc cttacaccct
     1441 taaaaacatt tattttagtt ggggtttcgc ttatcagctt aattatactt acaacctaca
     1501 ttattgcttt tacaaactta cgcgagtata tacccggcta tgctgatgta ggtatgaaac
     1561 gcaaccttat ttatttaagc ctgaaaaccg attcgcttac gcaaaaaatt attgcacagg
     1621 agcaatatat cagcaattta aatgaagtta taaatggcaa agccggtgct taccaaggtg
     1681 ttgataccaa tgcaaaagcg ctttatagcg atagtttatc gttaccaaaa tcggctgccg
     1741 atagtcaact gaggcaacta atcgaatcgc aagaccaata cgaccttgat tttatttcgc
     1801 aacaaaaaca tgctacgggc attgccggtt tcagctattt tacaccggtt acaggtacca
     1861 taaccgaaac atttaacccg gcattaaaac attttggtat tgatgtggtt gctgcaccca
     1921 atgagcctgt aaaatcaacc ttagatggaa ctgtggtatt tgcaggtttt accagtgcca
     1981 ccggttatgt tattgccgta cagcatagcg gcaatttatt tagcatctat aagcacaatg
     2041 ccggtttatt gaaatccgtt ggcgattatg taaatgcagg tgatgcaatt gccattattg
     2101 gcaacagtgg cgagttaagc caagggccac atctacattt cgaactttgg tataatggca
     2161 gtgcagtaaa cccaaccgat tatatgagct tttaaaaaca taacccttca aacgaattga
     2221 tgcaggcata ccattatgaa taatgcttat actttttaaa ttattttttc aaatttgcac
     2281 aacttaaaaa catctaaaac aatatttgta tggcacatgc tacaccaacc aacgacagta
     2341 aatttacaac caacctgttt ttaatttata tgctttctac agcggtgctt gtatcggcac
     2401 ttatatattg tgttggtgca acccatacta ttgaccatgc cagcaacccg cacaccggat
     2461 taaaagcccc tgctgttggc catggcggcc acgaggcaca cgactcccat gcggccgaca
     2521 ctaaaaccga accacatgct gccgaaaccc atgcagccgt taagcatgaa ttacccggtg
     2581 gcctgcatat agaagttgca gccggtagta ttgaagataa acttattgct tttatacaag
     2641 acccacatgc gcaagtgagt aaggatttat ggttcgattt taacgacttg ttatttaaaa
     2701 ccaatgaagc cgttttagaa ccgcagtcaa tgcaacaatt aaaaaatgta gctgcaattt
     2761 ttaaagcatt tccggcagtt aaagcaaaaa ttggtggtta taccgataat accggtgatg
     2821 cacaggccaa cttaaaatta tcagatgcac gtgccaaatc agtaatgcaa acattggtta
     2881 ccgaaggcat agcagccgat agattagagg ccgaagggta cggtcaggag catgccgttg
     2941 ccagcaacga tacagaagaa ggacgtgccc taaaccgcag aatttcatta agtgttcgcg
     3001 ctaaataatc aacaacatac attaataaat aaagccctgt tgccgtaaag cagtggggct
     3061 tatttttttt aaaccgcttt aaaaattaaa aatacgatgc aacgcagggt agctatttta
     3121 ggcagcacag gctcaattgg caaacaagct ttagaagtaa taagcatgca cagccaaagt
     3181 tttattgccg aggttttaac agcacaaagc aatgccgaaa ccttaattaa tcaagccctt
     3241 actttcaatc ccaatgtggt ggtaataggc gatgccaata aatacgaaac cgttaagcaa
     3301 gcattggcac ataccgatgt aaaagtatat gccggtgccg atgccattgc ccaggtagta
     3361 actatggata ccattgacat tgtactaaca gcattggttg gctatgcagg cttgttaccc
     3421 accattgcag caattaaagc cggtaagccc atagcattag ccaataaaga aaccttagtg
     3481 gtggccggtg aaattattac ccaactggct gcacaacata aagttgtcct actaccggtt
     3541 gatagcgaac actcagccat atttcaatgc ctgcaaggcg aatgggataa tgccatcgaa
     3601 aaaatttacc tcactgcatc gggtgggccg tttcgcggta aagacctgaa gtatttagta
     3661 agcgtaacca aggcacaggc attaaaacac cccaactgga gcatgggtgc aaaaattact
     3721 attgatagtg ccagtatgat gaataaaggt ttagagatga ttgaagccaa gtggttgttt
     3781 aatttgtcgg aaacgcaaat tgatgttgtg gtgcatccgc aaagcatcat acatagcatg
     3841 gtacagtttg aagacggcag tataaaagca caaatgggcc tgcctgatat
//
LOCUS       DAOMAA010000048         3853 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_16553_length_3853_cov_2.886519, whole genome shotgun sequence.
ACCESSION   DAOMAA010000048 DAOMAA010000000
VERSION     DAOMAA010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16553_length_3853_cov_2.886519"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..1227)
                     /locus_tag="PLO59_01175"
     CDS             complement(109..1227)
                     /locus_tag="PLO59_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HQU99732.1"
                     /translation="MKIGIIGTRGIPNNYGGLEQFAERVSVLLVKRGYEVLVYNPDFH
                     PYKQNNYQGVTVIHKWSPEQKIGTAGNFIYDYLCLKDAIDRKCDIVLACGYTTQSITY
                     FVAPIHKTKLITNIDGMEWWRSKYSATIRKLAKWFEKVAIEKSLALVSDNRGIEDYVQ
                     ETFGLKSYFIPYGADKNDKVDESVLKQFNLEKYKYNIMICRLEPENNIEVILDGVALS
                     KSPTKMYVAAGTNHKYAQYLINKYKGVDKIVFLGWVSGQELLNNLRNFSALYFHGHSV
                     GGTNPSLLEAMAGGAFIAAHGNKFNKHVLGDDALYFMNAQEAAAVIDNFNTLNQKRST
                     FVANNIHKIDTFYNWENIADMYARMFEEVASGKMTYTC"
     gene            complement(1331..2335)
                     /locus_tag="PLO59_01180"
     CDS             complement(1331..2335)
                     /locus_tag="PLO59_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007540612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQU99733.1"
                     /translation="MRIFITGVAGFLGSHLADRMLQLGHEVIGNDNFIGGYRDNINPN
                     IEFHEVDCMDLAKMRELTKGCDIIYHCASTAYEGLSVFSPHLVCTNTYQITMSMLSAA
                     IQNKVKRFVYCSSMARYGYQPVYPFTEDMDPKPIDPYGIAKRAAEITIENMARVHKFE
                     YAIAVPHNIIGARQKYDDPFRNVASIMINLMLQGRQPIIYGDGMQMRNFSFVGDCIDC
                     LERMAFQDNVNGEIINIGPDDEFVTINQLAATLGKILNFDVKPMYVADRPMEVKHASC
                     SADKARKLLNYKPQMTLEAGLIDMIDYIRKRGVLPFEYHLEVEIQNEFTPRTWADRLF
                     "
     gene            2646..3254
                     /gene="cysC"
                     /locus_tag="PLO59_01185"
     CDS             2646..3254
                     /gene="cysC"
                     /locus_tag="PLO59_01185"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012800870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HQU99734.1"
                     /translation="MSGNANIVKHNYRITQANRIALLNQQPLVLWFTGLSGSGKSTLA
                     DALEQRLWQDGIKTYLLDGDNIRFGLNKNVDFSDEGRKENIRRIGEVSKLFVDAGIVV
                     LTAFVSPFIADRQAVRDLLPEGRFVEVFVNTPIEVCEQRDVKGLYKKARAGEITDFTG
                     INSPYEAPLNPEVNIQTAQRNVSDCIEEIYQQVILKIKNVHD"
     gene            3247..>3853
                     /gene="cysD"
                     /locus_tag="PLO59_01190"
     CDS             3247..>3853
                     /gene="cysD"
                     /locus_tag="PLO59_01190"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009336 - sulfate adenylyltransferase
                     complex (ATP) [Evidence IEA];
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA];
                     GO_process: GO:0070814 - hydrogen sulfide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HQU99735.1"
                     /translation="MINYNLNHLKELESETIYIIREAAAQFENPCLLFSGGKDSIVMS
                     WMARKAFYPARIPFPLVHVDTGHNFPETIAYRDEYMKMIGANLIVGMVQDSIDSGRVK
                     EEKGPNASRNGLQTVTLLDTLEKHKFDAAMGGARRDEEKARAKERMFSHRDEFGQWDP
                     KNQRPELWLLLNGKKSVGEHFRVFPISNWTEMDVWQYILKEN"
BASE COUNT         1195 a          754 c          729 g         1175 t
ORIGIN      
        1 gtctgcacgt ttaatgcata accatattat tttgttgctt acatacttga ataaataaaa
       61 accgggcaaa taaaaatggt gtcggtttat ataattagcc tgttgtactt aacaagtgta
      121 agtcattttg cccgaagcta cttcttcgaa catacgtgca tacatatcgg caatgttttc
      181 ccaattataa aaagtgtcaa ttttatgtat gttgttagcc acaaaggtgc tgcgcttttg
      241 attaagcgta ttaaaattat caattaccgc tgcggcttcc tgggcattca taaaatacaa
      301 agcatcatcg cctaatacat gcttattaaa tttattgcca tgggccgcaa taaaagcgcc
      361 accggccatg gcttcgagca aactggggtt ggtgccgccc acactgtggc catgaaaata
      421 taaggccgaa aagttgcgta agttgtttag taattcctgt cctgataccc atcctaaaaa
      481 tacaatctta tcaacgcctt tgtatttgtt gattaaatat tgtgcgtatt tgtgattggt
      541 acctgcagcc acatacatct tagttgggct tttgctcagt gcaacgccat ctaaaataac
      601 ttcaatatta ttttcaggct caagcctgca aatcataatg ttatacttat acttttcgag
      661 gttaaattgt tttaaaaccg attcgtcaac cttatcattt ttatcggctc catacggaat
      721 aaagtaactt ttaaggccaa aggtttcctg cacataatct tcaataccac ggttgtcgct
      781 taccaatgcc agcgactttt cgatagccac tttttcgaac catttggcta acttacgtat
      841 ggttgccgag tacttactgc gccaccactc cataccatca atattggtta taagtttggt
      901 tttatgtatg ggtgcaacaa aataggttat actttgtgtt gtgtagccac aagccagtac
      961 aatatcacat ttacggtcaa tggcatcttt caaacataaa tagtcgtaaa taaaattacc
     1021 tgccgtacca atcttttgtt ccgggctcca tttatgtatt acggtaacgc cttgatagtt
     1081 gttttgttta tagggatgaa aatcgggatt atataccaac acttcataac cacgtttaac
     1141 caacaacacc gaaacacgct cggcaaattg ctctaaacca ccataattgt tgggtatgcc
     1201 acgcgtacct ataatgccta ttttcattta taaaacgatt gaattttaaa gcggcaaaga
     1261 aaaaaaaacc cgcaagtttt ttgcgggttt cttatttatt gaaagattat ttatcagtag
     1321 gattatgtta ttaaaataat ctatcggccc aggtacgtgg cgtaaactcg ttttgaattt
     1381 ctacttctaa atgatattcg aaaggcaaca caccgcgttt tctgatgtaa tcaatcatat
     1441 caattaaacc ggcttctaat gtcatttgcg gtttgtaatt taaaagctta cgtgccttat
     1501 cggccgagca gcttgcatgt ttcacttcca tggggcggtc ggcaacatac ataggcttta
     1561 catcaaagtt taaaatttta cctaaagtag cagccaactg gttaatggtt acaaactcat
     1621 catcaggacc aatgtttatg atttcgccat ttacattatc ctgaaacgcc atacgctcta
     1681 agcaatcaat acaatcgcct acaaatgaga agttgcgcat ttgcatacca tcgccataaa
     1741 taataggctg gcgaccttgc aacatcaggt taatcataat actggccacg ttacgaaacg
     1801 gatcgtcata tttttgacgg gcaccaataa tattatgagg cacagcaatg gcatattcga
     1861 atttatgcac gcgggccatg ttttcgatag taatttcggc tgcacgcttg gcaataccgt
     1921 atgggtcaat tggttttggg tccatatcct cggtaaaagg ataaaccggt tggtaaccat
     1981 aacgtgccat tgacgagcaa tacacaaaac gttttacttt gttttgaata gctgcactaa
     2041 gcatactcat ggtaatctga taggtgttgg tacaaaccaa atgaggcgaa aaaaccgaca
     2101 agccttcata agcggtgctg gcacagtgat atataatatc gcaacctttt gtaagctcgc
     2161 gcatttttgc caagtccata caatctactt catgaaactc aatgtttgga ttaatattat
     2221 cgcggtaacc gccaataaaa ttatcgttcc caattacttc atggcctaat tgtaacatac
     2281 ggtcggctaa atggcttccc aaaaatccgg ctactccggt aataaaaatt ctcatattaa
     2341 tgtttgtatt aaggtgtcct attttttttg cggctgcgaa aatacacctt tattcaaata
     2401 acaaaagcac ttataaatac atcttacaat taaaaaaact ttacggttat gttatttcac
     2461 atacccttta aacgcttatt tgatggcagt ttacatggct aatgggttta aaaacaggct
     2521 aaacttgaat aaattaacat gtcatagcag gcataataaa cataataacc tatagagatt
     2581 atagatttat gtattgaatt ttttttactt tcgcgcggcc taattaaaaa gcaggctatt
     2641 attatatgag tggcaacgcc aacattgtaa aacacaatta ccgcattacc caagcaaacc
     2701 gtattgcttt attaaatcaa caaccgttgg tgttgtggtt tacaggcctt agcggttcgg
     2761 gcaaaagcac tttggccgat gcattagagc aacgtttatg gcaagatggt attaaaacct
     2821 atttgttaga tggtgataac attcgttttg gcttaaataa aaatgttgat tttagtgatg
     2881 aaggccgcaa ggaaaacatc agacgcattg gcgaagtatc aaagcttttt gttgatgccg
     2941 gaattgttgt acttactgct tttgtatcgc cttttattgc cgacaggcaa gccgtgcgcg
     3001 atttattacc cgaaggtcgt tttgttgaag tgtttgtaaa cacacctatt gaagtatgcg
     3061 aacaacgcga tgtaaaaggt ttatataaaa aagcacgcgc aggcgagatt accgatttta
     3121 caggcatcaa ttcaccttat gaagccccgc taaaccccga agtaaatatc caaacagcgc
     3181 aacgcaacgt tagcgattgc attgaagaaa tatatcagca agtaatttta aaaataaaga
     3241 acgtacatga ttaattacaa tttaaaccat ttaaaagagc tggaaagcga aacgatttac
     3301 atcattcgcg aagctgccgc tcaatttgaa aacccctgct tattatttag cggaggtaaa
     3361 gattcgattg ttatgagttg gatggctcgt aaggcatttt atccggcacg cattccgttt
     3421 ccgttggttc acgttgatac cggacacaat tttccggaaa caattgccta ccgtgatgag
     3481 tatatgaaaa tgattggtgc taacctgatt gttggtatgg tacaagactc gattgacagc
     3541 ggacgtgtta aggaagaaaa aggccctaat gcatcgcgta atggcttgca aactgttact
     3601 ttattagaca ctttagaaaa acataagttt gatgcagcca tgggcggtgc cagacgtgat
     3661 gaagaaaaag cacgtgccaa agaacgtatg tttagtcaca gagacgagtt tggtcaatgg
     3721 gatcctaaaa accaacgccc agaactttgg ttgttactaa acggcaaaaa atcggttggc
     3781 gaacattttc gtgtattccc tatcagtaac tggactgaga tggacgtatg gcaatatatt
     3841 ttaaaagaaa att
//
LOCUS       DAOMAA010000049         3853 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_16554_length_3853_cov_2.567930, whole genome shotgun sequence.
ACCESSION   DAOMAA010000049 DAOMAA010000000
VERSION     DAOMAA010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16554_length_3853_cov_2.567930"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="PLO59_01195"
     CDS             complement(<1..479)
                     /locus_tag="PLO59_01195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQU99736.1"
                     /translation="MPRILRIINRLNLGGPTYNVAFLTKFMDARYETQLVSGAIDDTE
                     ASSNFILDKYNLKPVYVSEMKRAINPKDDYLAYQKIKKIIKEFKPDIVHTHMAKPGTV
                     GRLAALHCKVPVILHTFHGHYFHSYFSSTTTKLFLQIERYLAAKTNGIVVLSEVQKK"
     gene            complement(534..1610)
                     /locus_tag="PLO59_01200"
     CDS             complement(534..1610)
                     /locus_tag="PLO59_01200"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HQU99737.1"
                     /translation="MRKLDKYIIGKYLTTFFYCIGLIVLITIVFDISEKLDDFLEKKA
                     PLHAIIFDYYINFIPFFVNLFSPLFLFISVIFFTAQMANRTEIIAILNAGVSFTRLLK
                     PYMIASAVLALLSFVLGSYVIPHAKEKQLAFENKYIKNQYRFSTKNLHRQIKPGEFIY
                     FESYNNIDKEGYHFTYEIFKDKKLAYKLTAQKIKWDSTANQWRLENYLIRNIEPLHEK
                     FKKGNNLDTTFSFVANDFGRRPNFIEGMDSFELKAHINRERQRGADDIHFAEVEYNQR
                     FSFPYAMFVLTIIGVAIAYRKVRGGIGLQIVAGILLSFSYIMFMKVSTTMATNSNVPA
                     QFAVWIPNIIYSVIALIMVKRAQL"
     gene            complement(1630..2760)
                     /gene="tgt"
                     /locus_tag="PLO59_01205"
     CDS             complement(1630..2760)
                     /gene="tgt"
                     /locus_tag="PLO59_01205"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008239755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HQU99738.1"
                     /translation="MQFDLQITDTLSKARAGKIFTDHGIIETPIFMPVGTAATVKGVH
                     QNELQHDIKAQIILGNTYHLSIRPGMDVMQQAGGLHKFMNWHKPILTDSGGYQVYSLA
                     GTRKIKEEGVKFQSHIDGATLFFTPEHAIDIQRQIGADIVMAFDECTPYPCEHKAAKR
                     SMELTHRWLQRCVNHFNAHKPIYGHNQTLFPIVQGSTYADLRKQSAEFVSGINAHGNA
                     IGGLSVGEPAANMYEITDCVTDILPKSKPRYLMGVGTPVNILECIGLGIDMFDCVMPT
                     RNARNGMLFTSEGIINIRNEKWKNDFSAIDNNANSVVDTCYSKAYLRHLVISKEILGA
                     QIATLHNLAFYLDLVKQARQHILNGSFVKWKNEMIPKLSRRL"
     gene            complement(2863..>3853)
                     /locus_tag="PLO59_01210"
     CDS             complement(2863..>3853)
                     /locus_tag="PLO59_01210"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HQU99739.1"
                     /translation="TTETAITKNGYDMMPYTDNFYPKSMNSLIFYAEIYNADKVLGSN
                     NGYVLSYYLINDESKIILDEYKVTKREIAKPTTYGMSEINIEKLASGNYNLIIELRNK
                     NNELIGFTERYIQRSNFIAPANSDIAISNIDVAQTFVNAITDADTLKEFVACLRPICN
                     PTQTVWQDNQLKLADLRMMQQFFYDFWFKKNPTAPEKAWLDYKAQVALVHQIYGDRFT
                     KGYTTERGRVYLQYGAPNSVTKRDHEPDAYPYEIWQYYKIKNQTNRKFVFYNPNLAGN
                     DYRLLHSDMNGEVMNNNWSMDLHKRNSQSLDYDVEKRRQYIGNQSQESFDVPR"
BASE COUNT         1102 a          687 c          710 g         1354 t
ORIGIN      
        1 tcttttttct ggacttcgct taaaaccaca ataccatttg ttttagcagc taagtaacgt
       61 tcgatttgta aaaataactt agttgtagta ctgctaaaat acgagtgaaa ataatggcca
      121 tgaaatgtgt gtagtataac aggtacttta cagtgcaaag ctgccaaacg tccaacggta
      181 cccggttttg ccatgtgtgt atgcacaatg tcgggtttaa attcttttat aatttttttt
      241 attttttgat aggccaggta atcgtctttg ggatttattg cccttttcat ttcggagaca
      301 tatacaggtt taagattgta tttgtctaaa atgaaatttg aactggcctc agtatcgtct
      361 atggctcctg aaactaattg ggtttcataa cgcgcatcca taaatttggt taaaaaagca
      421 acgttgtatg taggccctcc taaatttaaa cggtttatta ttcgaagaat gcgtggcatt
      481 aactcaatat gtttttcgta acgggtatta tacgtagttt cttattggtg ttattacagt
      541 tgcgcgcgtt ttaccattat aagggcaata accgaataaa ttatattggg tatccaaacc
      601 gcaaattgtg ccggtacatt gctattggtt gccattgtgg tagatacttt cataaacatg
      661 atgtaactaa agcttaataa aataccggca actatttgca gcccaatgcc acctcttact
      721 tttcggtaag caattgctac acctataatt gtaagcacaa acattgcgta tggaaatgaa
      781 aatcgttgat tgtactcaac ttcggcaaaa tgtatatcgt cagccccgcg ttgacgttcg
      841 cggttaatgt gagctttaag ttcgaaacta tccatgcctt caataaaatt aggtctgcgg
      901 ccaaaatcat ttgccacaaa cgaaaatgtt gtatccaggt tgttgccttt tttaaacttt
      961 tcgtgtaatg gttctatgtt tcttattaaa taattttcaa gtctccattg atttgctgta
     1021 ctatcccatt ttattttttg ggctgttagt ttatacgcca gctttttatc tttaaaaatt
     1081 tcataagtaa aatggtatcc ttctttatca atgttattgt aactttcgaa gtaaataaac
     1141 tcaccgggtt ttatttgcct gtgtaaattt ttggtgctga aacggtattg gttttttatg
     1201 tatttatttt cgaaagccag ttgtttttct ttagcatgtg gtataacgta gctgcctaaa
     1261 acaaaggaaa gtaatgccaa tactgccgat gctatcatgt atggtttaag caagcgtgta
     1321 aaacttaccc ctgcatttag tatggcaata atttcggtac ggtttgccat ttgtgccgta
     1381 aaaaaaataa ccgatataaa tagaaatagt gggctaaaca ggtttacaaa aaatggaata
     1441 aagtttatgt aataatcaaa aataattgcg tgaagcgggg ctttcttttc taaaaaatca
     1501 tccagttttt cgcttatgtc gaatacaata gtaattaaca ctattaaccc aatacagtaa
     1561 aaaaatgtag ttaggtattt gccaataata tatttatcga gtttgcgcaa gcgtagcgtt
     1621 ttattaatgt tataacctac ggcttagttt gggtatcatt tcgtttttcc attttacaaa
     1681 actaccgttt aaaatgtgtt ggcgtgcttg ttttaccaag tctaaataaa atgcaaggtt
     1741 gtgtaaagta gctatctgcg cgcccagtat ttctttactt ataaccaaat gccttaaata
     1801 agctttactg tagcatgtat caaccactga attagcatta ttatcaattg ctgaaaaatc
     1861 gtttttccat ttttcatttc tgatattgat tattccttca gatgtaaata acataccatt
     1921 gcgtgcattg cgtgttggca ttacacaatc aaacatatca attcctaaac caatacattc
     1981 taatatatta acgggtgtac ctacgcccat caggtagcgt ggtttgcttt ttggcaatat
     2041 atcggtaaca caatctgtta tttcatacat attggctgca ggttcgccaa cgctcaaacc
     2101 gccaatggca ttaccatggg cattaatgcc gctcacaaac tcggccgatt gtttacgtag
     2161 gtcagcatag gtgcttcctt gcacaatagg aaacaaggtt tggttatgcc cgtaaattgg
     2221 tttatgtgca ttaaaatgat ttacacaacg ttgcaaccat cggtgcgtaa gttccatgct
     2281 gcgtttggca gctttatgtt cgcaagggta gggcgtgcac tcatcaaatg ccattacgat
     2341 atcggcacct atttgacgtt gaatatctat tgcatgttca ggtgtaaaaa acaaagtggc
     2401 tccatcaata tgcgattgaa attttacacc ttcttctttt atttttcgtg tgccagctaa
     2461 tgaataaacc tggtagccgc cactatcagt aagtatgggt ttgtgccagt tcataaactt
     2521 atgcagccca ccggcttgtt gcattacatc catacccggc ctgatactta ggtggtatgt
     2581 attgcccaaa attatttgtg cttttatatc gtgttgcaat tcgttttgat gtacaccttt
     2641 tactgttgca gcggtaccca caggcataaa aatgggtgtt tcgataatac catgatcagt
     2701 aaaaatttta ccggcacgtg ctttactaag ggtatcggta atttgtaagt cgaattgcat
     2761 aaattaaata tggcggccaa attaataaat gctttacatg gccaaaaact tatgttaccg
     2821 aaaagcaata tggattggtt gctagtgatt cactaaaaaa aattaccgag gtacatcaaa
     2881 cgactcttga ctttggttgc ctatatattg cctgcgtttt tctacatcat aatctaacga
     2941 ctgcgaattg cgtttgtgta aatccatact ccagttgtta ttcatcactt cgccattcat
     3001 atcggaatgc agtaaacgat aatcgttacc tgctaagttt ggattgtaga atacgaattt
     3061 gcgattggtt tggtttttta ttttgtagta ctgccatatt tcgtaagggt aggcatcggg
     3121 ttcgtggtcg cgtttggtaa cagagtttgg tgcaccatat tgtaagtaaa cacggccgcg
     3181 ttcagtagtg tatccttttg taaatctatc gccataaatt tgatgtacca aggcaacctg
     3241 tgctttgtaa tctaaccaag ctttttccgg tgctgttgga tttttcttaa accagaaatc
     3301 ataaaaaaat tgttgcatca tacgtaaatc agccagtttc agctggttgt cttgccatac
     3361 ggtttgtgtt gggttgcata taggtcgcag gcatgcaaca aactccttta atgtatcggc
     3421 atcggttatt gcatttacaa aagtttgtgc aacatcaata ttactaattg ctatgtcgct
     3481 atttgcaggt gcaataaaat tacttctttg tatgtatcgt tctgtaaaac caataagctc
     3541 attgttttta tttctcaatt caataatcag attataatta ccggaagcta atttttcgat
     3601 attaatttca ctcatcccgt aggtcgttgg cttggcaatt tcgcgtttgg ttactttgta
     3661 ctcgtctaaa attattttgc tttcgtcatt aattaggtag tatgataata catatccatt
     3721 attgctgcct aataccttgt cagcattata aatttcggca taaaatatta agctattcat
     3781 gctttttgga taaaaattat cggtataggg catcatgtca taaccatttt tagtgatggc
     3841 agtttctgtt gta
//
LOCUS       DAOMAA010000050         3804 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_16953_length_3804_cov_2.806615, whole genome shotgun sequence.
ACCESSION   DAOMAA010000050 DAOMAA010000000
VERSION     DAOMAA010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3804
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16953_length_3804_cov_2.806615"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2648
                     /locus_tag="PLO59_01215"
     CDS             <1..2648
                     /locus_tag="PLO59_01215"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HQU99740.1"
                     /translation="NPVANGNSISWSTLDKEVKAKLAEINANNGNIRIVTASINSPST
                     LKVLADFKAAYPTTEVVTYDAISFNGILKANELSFGKRVMPSYNIDKAKVIVGIGCDF
                     IGNWIAPVTLAAQYAKNRKLKGGNKSMSRHIQIESTLTLTGSNADDRIRVKPSEQGSI
                     VVSLYNKVASKAGASTVTGGNATYDAMLTKLADELWANKGNAVLMCGSNSVAEQTLVN
                     GINVLLGAYDAIIDLDKHCNLKKSNDEAFAKLVTDMNGGNVAALIVYNCNPAYTAPNA
                     KEFIEGIKKVSLSVSFAEKQDETAALCKYLAPDCHYLESWNDAEPFNGVYSFCQPTIQ
                     KLYKTRQAQESLLAWSGAPIASFHDYIATYWQGLAPATSTPNQFWTQIIQTGVYESGI
                     PTKTTINFVGSVADAASKIATQKSAGEIEVLLYEKTGIGNGNQANNPWLQELPDPISK
                     VCWDNYVCISPKMAATKSLAQGNVVKVSVGSQSLTLPVYVQPGIAEGTIAIALGYGRQ
                     KAGDADNNVGKNAYPLISFVDGSMQYYAAGKLDKTTEDNYVLAATQTHHTMMGRAIVK
                     ETTLAEYAKNPKAGNPDVLIETPEGKQRPKDIDIWATTTQPGFDRPNHSWGLGIDLNA
                     CIGCGACVVACNAENNVAVVGKDEVNRAREMHWIRIDRYYSSDTTKENAASKNLGALS
                     MYGEMEKPSVENPKVFFQPVMCQHCNHAPCETVCPVVATTHSSDGLNMMAYNRCVGTR
                     YCANNCPYKVRRFNWFNYAENAKFPYTQYHQSERMVLNPDVTVRSRGVMEKCSMCIQR
                     IQSGKLTAKKESRRPVDGEINTACAQTCPTNAITFGDFTDKDSQISVMFKEDRTYWLL
                     EEINVQPSVFYQTKVWNHDNKTEKA"
     gene            2707..>3804
                     /gene="nrfD"
                     /locus_tag="PLO59_01220"
     CDS             2707..>3804
                     /gene="nrfD"
                     /locus_tag="PLO59_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010177372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HQU99741.1"
                     /translation="MSNVESALRPALITGDKTMHQITEDICRPIEGKANKYWWMAFSV
                     SCAALLWWIICVSYTVGTGLGVWGLNKTVGWAWDITNFVWWVGIGHAGTLISAILLLF
                     RQKWRLSINRSAEAMTIFAVICAASFIVAHMGRPWVAYWPLPLANQFGSLWVNFNSPL
                     VWDVFAISTYFSVSLVFWYSGLLPDFAVIRDRAKTKSMKFWYTLLSFGWSGTAKNWQR
                     FEEMSLVLAGLSTPLVLSVHSVVSMDFATSIIPGWHTTIFPPYFVAGAIFSGFAMVQT
                     LLIIVRKVLGMEDYITMYHIGMMNRIIVVTGSIVGVAYITEFFIAWYSGVEYESYAFI
                     NRATGPYWWAYWSMMTCNVISPQLFWSAKLRR"
BASE COUNT         1208 a          698 c          809 g         1089 t
ORIGIN      
        1 aaaatccggt tgctaatggt aattcaattt cgtggagtac gctcgataaa gaggttaaag
       61 ccaagttagc cgaaattaat gccaacaatg gaaatatcag aatagttacg gcatcaataa
      121 attcgccttc gactttaaaa gttttggctg attttaaagc ggcttaccca accactgaag
      181 tggttactta tgacgctata agtttcaatg gaattttaaa agcaaatgaa ttatcatttg
      241 gtaagcgggt aatgccaagc tataatattg ataaagctaa agtaatagtg ggtattggtt
      301 gcgattttat aggaaactgg attgcaccgg ttacacttgc ggctcaatac gcaaaaaacc
      361 gtaagctaaa aggtggcaac aaatcaatgt cgcgccacat tcagatagaa tcaacactta
      421 ctttaaccgg tagtaatgct gatgatagaa tacgcgtaaa accgagcgaa caaggcagta
      481 tagtagtttc gctttataat aaagttgcaa gcaaggctgg ggcatcaact gttaccggtg
      541 gtaatgccac ttacgatgcc atgcttacta aattggctga tgaactgtgg gcaaataaag
      601 gtaatgcagt gctaatgtgt ggttctaaca gcgttgctga acaaacgctt gttaatggta
      661 ttaatgtttt attgggagct tacgatgcta ttattgattt agataagcat tgtaatctaa
      721 agaaaagcaa tgatgaagca tttgcaaaat tggtaaccga tatgaatgga ggcaatgttg
      781 cagcattaat agtttataac tgtaatccgg catacactgc accaaatgcc aaagaattta
      841 ttgaaggcat aaaaaaggtt tcgctttcag tttcatttgc cgagaaacaa gatgaaacag
      901 cagcgttatg taaatactta gcccctgatt gtcattatct ggaatcgtgg aatgacgctg
      961 aaccatttaa tggtgtttat agtttttgtc aaccaactat tcaaaaactt tacaagacgc
     1021 gtcaagcaca ggaaagttta ttggcatggt cgggcgcacc cattgcatcg ttccatgatt
     1081 atatagcaac ttattggcag ggattagcac ctgcaacatc aacacctaat caattttgga
     1141 ctcaaataat ccaaacaggt gtttacgaaa gcggaattcc tacaaaaacc acaataaact
     1201 ttgttggttc agttgccgat gcagcttcaa aaatagccac acaaaaaagt gcgggtgaaa
     1261 tcgaagtact gctttacgaa aaaacaggta ttggcaatgg taaccaagca aataaccctt
     1321 ggctacagga gttgcccgac ccgatttcga aagtttgctg ggataattac gtttgtattt
     1381 cacccaaaat ggcagcaacc aaatcattgg ctcaaggtaa tgttgtaaaa gtttctgttg
     1441 gtagccaatc gcttacactt cctgtatatg tacaacccgg tattgccgaa ggtaccatag
     1501 ccatagcact tggatatggc cgacaaaaag ctggtgatgc agacaataat gttggtaaaa
     1561 atgcatatcc attaattagt tttgtagatg gaagtatgca atactatgct gccggtaaat
     1621 tagacaaaac cacagaagat aattatgtgt tggcagccac acaaacccat cacaccatga
     1681 tgggtcgtgc catagttaaa gaaactacat tagccgaata cgccaaaaac ccgaaggctg
     1741 gtaaccccga tgtgttgatt gaaacaccgg aaggaaaaca aagacccaaa gacattgata
     1801 tatgggcaac aaccacacaa cctggtttcg accgacccaa ccattcatgg ggtttaggaa
     1861 ttgacttaaa tgcttgtatt ggatgtggtg catgcgttgt tgcatgtaat gccgaaaata
     1921 atgtggccgt tgttggtaag gatgaagtga accgtgcccg cgaaatgcac tggattcgta
     1981 ttgacagata ttatagttct gatacaacta aagaaaatgc agcttccaaa aatttgggtg
     2041 cactttcaat gtatggcgaa atggaaaagc catcggtaga gaatccaaaa gtgtttttcc
     2101 aaccggtaat gtgccaacat tgtaaccatg caccttgcga aactgtatgt cctgttgtag
     2161 ctacaacaca cagcagcgat ggattaaata tgatggcata caaccgttgt gtaggaacta
     2221 gatattgtgc aaataactgc ccttataaag taagaagatt taattggttt aactatgccg
     2281 aaaacgctaa gttcccatac acacagtatc atcaatcaga acgcatggtt ttaaaccctg
     2341 atgttacagt acgctcacgc ggtgttatgg aaaagtgttc aatgtgtatt caacgtatcc
     2401 aatcaggaaa actcacagct aaaaaggaaa gccgcagacc ggttgatggt gaaataaaca
     2461 cggcttgtgc gcaaacatgc ccaaccaatg ccataacttt tggtgatttt actgataaag
     2521 acagtcaaat ctccgtaatg tttaaagagg acagaaccta ttggttgttg gaagaaataa
     2581 acgttcagcc ttcggtattt tatcaaacca aggtctggaa tcatgataac aaaactgaaa
     2641 aggcttaata ataaaataga atataacttt aaaaggttat aaataacaca taaacaaaat
     2701 ttaaatatgt cgaacgttga atcagctttg aggccagccc tcatcacagg tgataaaaca
     2761 atgcatcaaa ttaccgaaga tatttgccgc cccattgaag ggaaagcaaa taaatactgg
     2821 tggatggcat tttctgtttc atgcgctgct ttattatggt ggattatctg tgtatcctat
     2881 actgttggaa caggcttagg agtatgggga ttaaacaaaa ctgtgggttg ggcatgggac
     2941 atcaccaact ttgtttggtg ggtgggtatt ggccatgccg gtacacttat ttctgcaatc
     3001 ttacttttgt tccgtcaaaa atggaggctt tcaataaata gatcggcaga ggcaatgacc
     3061 atatttgccg ttatttgtgc agcatcattt attgttgcgc acatgggccg cccctgggtt
     3121 gcttattggc cattaccttt agctaatcaa tttggttcat tgtgggttaa tttcaactca
     3181 cctttggttt gggacgtttt cgccatttca acatactttt ctgtatcgct tgttttttgg
     3241 tattcgggct tattacctga ttttgctgtt atacgcgata gagctaaaac aaagtcaatg
     3301 aaattttggt acactttact atcgtttgga tggtcgggta ctgcaaaaaa ctggcaacga
     3361 tttgaagaaa tgagcttagt acttgcaggc ttatcaaccc cattagtact ttcagttcac
     3421 tctgtggtat caatggactt tgctacctca ataatacccg gatggcatac caccattttc
     3481 ccaccatact ttgttgccgg agctatattt tcaggatttg caatggttca aacgctctta
     3541 ataattgtta gaaaggtatt aggaatggaa gactacatta ctatgtacca tattggcatg
     3601 atgaatcgaa ttattgtggt taccggttca atagtaggtg tggcttatat aacagagttt
     3661 tttattgcat ggtactcagg tgttgagtat gaatcctatg catttataaa cagagccaca
     3721 gggccatatt ggtgggcata ctggagtatg atgacttgta atgttatctc accgcagtta
     3781 ttctggtcgg caaaacttcg aaga
//
LOCUS       DAOMAA010000051         3794 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_17020_length_3794_cov_2.861995, whole genome shotgun sequence.
ACCESSION   DAOMAA010000051 DAOMAA010000000
VERSION     DAOMAA010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3794
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17020_length_3794_cov_2.861995"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="PLO59_01225"
     CDS             <1..526
                     /locus_tag="PLO59_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015029213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99742.1"
                     /translation="IKQLESLNLDAEVLQAEIDNVTAKDCLCEGLGASALINEGIHPA
                     HGLTAVAICPGPNLAYFSNTFTLSQMVDHIYGRINILNQQKRPNMFVNELSLYVDYLK
                     KEIEKCAKTISEKQLKYFNVFRNNLLSGIDYYVNLIPALKLESTTYTVQLKQDLMDAR
                     TKLNAMVIAPLVAL"
     gene            complement(580..>937)
                     /locus_tag="PLO59_01230"
     CDS             complement(580..>937)
                     /locus_tag="PLO59_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD-dependent epimerase/dehydratase"
                     /protein_id="HQU99743.1"
                     /translation="INKLMFDANFFKRIQINGNGAQYRSFIHIDKVTQVLTKLCTTHI
                     ESGIYNLVDKNLDINTVVETLQDIYPGMEFQFLNQHIAMPQIQVTLNGKLNKFISIPE
                     THFKTELLTFKNRFAF"
     assembly_gap    938..947
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<948..1504)
                     /locus_tag="PLO59_01235"
     CDS             complement(<948..1504)
                     /locus_tag="PLO59_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase"
                     /protein_id="HQU99744.1"
                     /translation="MKILITGGAGYIGAELSKSLAAQKEVTQIIVYDNLSRANYNLFF
                     GEAVFKNKLQFIQADILDNRSLRKALKDVDVVYHLAAKVTTPFSNLDASQFEQINHWG
                     TADLVYALEENDIKKFVFISSSAVYGHSAEMVDELTPTNPMGFYGISKLRAEGHVMRL
                     FDNMHTIILRCSNVYGYSTNMRFDAV"
     gene            complement(1508..2164)
                     /locus_tag="PLO59_01240"
     CDS             complement(1508..2164)
                     /locus_tag="PLO59_01240"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011584349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HQU99745.1"
                     /translation="MTVSEIGRQIENYKKRGLSLFTTCSFQSHSIVLLHILTTIDKNI
                     TVYFINTGFHFTETIAYKNQIAALLKINVKSLSSHVPKSLQKDMNNQFLFASDADHCC
                     YINKTMPVNGLLHMYDVWINGVRADQSSTRKNFKTEEPTPHHAIRFHPMLDWTPKMIY
                     DYAKTHNLPKHPLEEKGFFSIGCEPCTHKFEISSRGGRWYGLKKTECGLNTDLIADKK
                     "
     gene            complement(2190..3197)
                     /locus_tag="PLO59_01245"
     CDS             complement(2190..3197)
                     /locus_tag="PLO59_01245"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HQU99746.1"
                     /translation="MKKLLVIIIITLIIVVVNACKKEGDTLPPFNPTPLTIKLPGNLP
                     VMNIPASNPTTVEGVALGRMLYYDKIMHPNGSMACASCHLQSEGFTKKGTNILPHINL
                     GYGNKFLWNGEIEGTLEDAMIFEVEDFFKTNISKLQNDDTYPLLFYKAFGSTEIDSKK
                     CAYALAQFLRTLVSGNSKFDKYLRHELTLTADELTGLTIFFSERGDCFHCHSLSLMGD
                     GNRHNIGLDSIFNGVNAGYYNVTANPADLGKFKTPSLRNCGIRTSFMHDGRYTKLDEV
                     IEHYNSKVKNSASLDPVLTKPGKAYGLNLTNSEKEKLKAFLLTLTDTVYLSDTAFASP
                     F"
     gene            complement(3208..>3794)
                     /locus_tag="PLO59_01250"
     CDS             complement(3208..>3794)
                     /locus_tag="PLO59_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99747.1"
                     /translation="FDTLGYYNAAGNNYSISNLIFYLSAFEFENTTGQIYKFNDVYLV
                     DVKNKNNLNLSFTDLPAGTYKRVTFLIGLDSLHNLSNALPNTIENNNMAWPPAMGGGY
                     HFIKLEGKFMDQNQMFGMAIHLGRNQNCVVINITQNISLKPEMNRINLSMNINEWFKN
                     PHIYDLNIDGNFTMSDSAAMAKIKQNGSDVFTIN"
BASE COUNT         1136 a          673 c          697 g         1278 t
ORIGIN      
        1 aattaaacaa ttagaaagtt tgaatttaga tgctgaagta ctgcaagccg aaattgataa
       61 tgtaactgca aaagattgtt tatgcgaagg cttgggtgct tcggcattaa taaatgaagg
      121 catacatccg gctcatggtt taactgctgt agcaatatgt ccggggccta acctggctta
      181 tttttcaaac acctttacat taagtcaaat ggtagatcat atatacggac gtataaatat
      241 tttaaatcag caaaagcgcc caaatatgtt tgtaaacgag ctaagtttgt atgttgatta
      301 ccttaaaaag gaaattgaaa aatgcgccaa aacaatttct gaaaaacagc ttaaatattt
      361 taatgttttc agaaacaatt tattaagtgg tattgattat tatgtaaact taatacctgc
      421 gcttaaactc gaatcaacaa cttatacggt gcagttaaaa caggatttga tggatgcacg
      481 tacaaaactt aatgcaatgg ttattgcacc gctggttgca ttataaacag gtgccatata
      541 cgtaatttgt aattaaaagt atttaatgct aactgcctgt taaaacgcaa accggttttt
      601 aaatgttagc agttcggttt tgaaatgggt ttcaggtatt gaaataaact tgtttagttt
      661 tccgtttaat gttacttgaa tttgaggcat agcgatatgt tggtttaaaa actgaaactc
      721 catgccagga taaatgtctt gtaaggtttc taccaccgta ttaatatcta aattcttgtc
      781 aactaagttg taaatgcctg attcgatatg ggttgtacaa agcttggtta atacttgcgt
      841 tactttatca atatgaataa atgagcgata ctgggcaccg ttaccattta tttggatgcg
      901 cttaaaaaaa ttcgcatcaa acattaattt attaattnnn nnnnnnnaca gcatcaaatc
      961 gcatgttggt actataacca tacacattac tgcaacgtag tatgatggta tgcatgttat
     1021 caaacaaacg cattacatgt ccttcggcac gtaatttact tatgccgtaa aagcccattg
     1081 gattggttgg cgtaagctca tcaaccattt cggccgaatg gccataaaca gccgatgaac
     1141 tgataaaaac aaattttttt atatcgtttt cttccagtgc atacactaaa tcagcagtgc
     1201 cccaatgatt tatttgttca aactgactgg catccaggtt cgaaaacggt gtagtaactt
     1261 tagctgcaag atgatatact acatcaacat cttttaatgc tttacgtaaa ctgcgattgt
     1321 ctaaaatatc agcctgtata aattgtagtt tgtttttaaa aacggcttct ccaaaaaaca
     1381 aattgtagtt ggcgcgactt aaattatcat acacaattat ttgtgtaact tctttttgtg
     1441 cagctaacga tttcgaaagt tcagcaccaa tgtatcctgc accaccggtt attaatattt
     1501 tcataagtta ttttttgtcg gctattaaat ctgtatttaa accacactcg gtttttttta
     1561 aaccatacca ccgtccgcca cgtgaactga tttcaaattt atgcgtacag ggttcgcaac
     1621 cgatgctgaa aaatcctttt tcttctaacg gatgtttggg taaattatgg gtttttgcat
     1681 aatcgtaaat catttttggt gtccagtcta acatgggatg aaacctaatg gcatggtgtg
     1741 gtgtaggctc ctctgtttta aaatttttac gtgtactgct ttggtcggca cgtacgccat
     1801 ttatccacac atcgtacatg tgcagcaagc cattaacggg catggttttg tttatataac
     1861 agcaatggtc ggcatcggat gcaaataaaa attggttgtt catatccttt tgcaaactct
     1921 tgggtacgtg actcgacaag cttttgacat taattttcag cagtgcagct atttgatttt
     1981 tatacgctat ggtctcggta aaatgaaaac ctgtatttat aaaataaacg gttatgtttt
     2041 tgtcaatggt tgtaagtatg tgcagtagca caatgctatg tgattgaaac gaacaggtag
     2101 taaaaagtga aagtccgcgc tttttataat tttctatttg cctgccaatt tcagatactg
     2161 tcatatttca aaaaatttat cgtttgtgtt tagaacggac tggcaaatgc tgtatcagat
     2221 aaataaacag tatcggtgag agtaagcaga aatgctttta gtttttcttt ttcgctattg
     2281 gttaaattta aaccatacgc tttgccgggt ttggtaagca cagggtcgag cgatgccgaa
     2341 tttttaacct tgctattgta atgttcaatt acttcgtcca gtttggtgta acgtccatcg
     2401 tgcataaatg atgtgcggat accgcagttg cgaagtgaag gtgttttaaa tttacccaga
     2461 tcggccggat tggcagtaac attataatag ccggcattta ctccgttaaa aatagagtct
     2521 aagccaatgt tatgcctgtt tccatcgccc attagtgata gcgaatgaca atgaaaacaa
     2581 tcgcctcgct cgctaaaaaa tatggtaagc ccggttagct catcggctgt taatgtaagc
     2641 tcatgacgca gatatttatc gaatttagaa ttgcccgaaa ccaaggttct taaaaattgc
     2701 gccaatgcat aagcacattt tttcgaatca atttctgtgc ttccaaacgc tttgtaaaac
     2761 agcaatggat aggtatcatc gttttgtagc ttgctgatgt tggttttaaa aaagtcttca
     2821 acttcgaaaa tcatcgcatc ttcaagtgtg ccttctattt cgccattcca caaaaactta
     2881 ttgccatatc ccaaattaat atgtggtaaa atattggtac ccttttttgt aaaaccttcc
     2941 gattgtaaat gacaactggc acatgccatg cttccgttag ggtgcataat tttatcatag
     3001 tataacatgc ggcccaatgc caccccttca actgtggtgg ggttgctggc tggtatattc
     3061 ataaccggca aatttccggg tagttttata gttaatggtg tggggttaaa aggcggtaac
     3121 gtatcaccct cttttttaca ggcatttact actacaatta tgagcgttat gattatgatt
     3181 accagcagtt ttttcatttc tttttgctta gtttattgta aacacatccg atccgttttg
     3241 ctttattttt gccatggcag cactgtcaga catggtaaaa ttgccgtcaa tattcaaatc
     3301 atatatatgc ggattcttaa accattcatt aatattcatg ctaagattaa tgcggttcat
     3361 ttcgggctta agcgaaatgt tttgtgtaat atttattact acacaatttt ggttacgccc
     3421 caaatgtatg gccataccaa acatttgatt ttggtccata aacttgcctt ctaattttat
     3481 aaagtgataa ccaccaccca ttgccggtgg ccaggccata ttgttgtttt ctatcgtatt
     3541 gggtaatgca ttgctaaggt tgtgtaaact gtcaagacca attaaaaacg taactcgctt
     3601 ataagtgccg gccggtaaat cagtaaaact taagtttaaa ttatttttgt tttttacatc
     3661 aaccagatac acatcattaa atttataaat ctggcctgtt gtgttttcga attcaaatgc
     3721 cgataaataa aatatcaggt tcgaaatact gtagttgttg cctgcagcat tgtaatatcc
     3781 cagtgtgtca aatt
//
LOCUS       DAOMAA010000052         3787 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_17084_length_3787_cov_2.875938, whole genome shotgun sequence.
ACCESSION   DAOMAA010000052 DAOMAA010000000
VERSION     DAOMAA010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3787
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17084_length_3787_cov_2.875938"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="PLO59_01255"
     CDS             <1..769
                     /locus_tag="PLO59_01255"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HQU99748.1"
                     /translation="LGENAFTSGCISLQKANDLVKTLQAFKLLIEVHNPIAYRACATS
                     AMRDASNGKQILERISNEANIDVELIDGSTEARLIYMNHIEENLDRNSSYLYIDVGGG
                     STELTLFSQNQHIFSKSFNIGTIRMLNEQIEKAYWADFKDWIKSATTKFYPVVAIGSG
                     GNINKLHKIARLKDSKPLTYTKLEMLFEEINSYTYQERIELLGLKPDRADVIIPACKI
                     FLSVMKNASIDKIFVPEIGLSDGIVHELYESFKTQKT"
     gene            775..981
                     /locus_tag="PLO59_01260"
     CDS             775..981
                     /locus_tag="PLO59_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99749.1"
                     /translation="MKDFIITFIVAFVLFKVLSSIMDMRKQPNKGNNGNTTQRHVGDI
                     TIEETQEDKNKTNQTGYTDYEEIK"
     gene            1184..3544
                     /locus_tag="PLO59_01265"
     CDS             1184..3544
                     /locus_tag="PLO59_01265"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HQU99750.1"
                     /translation="MFQGKTLSQHDIAQWEGAAKEIIDFRNTEHAEPLWTNRMFGGMP
                     AYQISTLYPSNLIAHVNTFLTFGLPSPAYLIFMGLLSFYILMLTLKVDYRLAIGGAIS
                     YAFVSFNVVVIQAGHNSEAHAIVLFPLVIAGVLMVFNKNYWAGGALTAIGLALQLYAN
                     HLQITYYTALTVLCLVIARSIYAITKNETKHLLKSALVLSIAATLGVLPNITNLLVTN
                     EYGKASTRGSSELTEKKSSTGLDKDYALNWSNGKRDAVTLLIPDATGGASTKKLDHKS
                     ATYKALESNGVLDQAAGLLDAAPVYWGGLPSTSGPVYAGAIIILLCVIGMLTLKGPDK
                     WWLLIATLLSVSLSWGRYFLPLTDLFFNYFPAYNKFRSVSMIMVIASFCLPLLAIMGL
                     QGFIKLTDASERKKILMRALSFTGGFCVIVLLGSYMIDYSAESDKYYANAPWLIAALK
                     QDRAAILRADTLRSLTFVLLAFGLLWFYINQKLKLHLLYMLLSVLFLFDMWPVAKRYL
                     NNDSFAEKTAEETPFTPTQADMQILQDKSLGYRVMNTTVSTFNDAGTSYFHNSIGGYH
                     GAKLKRYQELIEYQIGKGNQAVIDMLNTKYFIFQNKQSGLPEAQINPQACGNAWFVSN
                     IKFVVNADAEMAALDKFNPKTEVFVDQRFKSQVGSIPNQTDSSASIVLTSYKPNYISY
                     SSQTNTEQFAVLSEIYYDKGWNVYVDGKPTDYVRCNYVLRGMKVPAGNHKIEFKFEPA
                     TYAMGEKISLAGSLLLFAGCLAVLFMEFKKTKQSPETLPAGKKGKN"
BASE COUNT         1249 a          669 c          716 g         1153 t
ORIGIN      
        1 attaggcgaa aacgctttta caagtgggtg tataagttta caaaaagcaa atgatctggt
       61 aaaaacgctt caggcattta agttacttat cgaagtacat aatccaatag cataccgtgc
      121 atgtgcaaca tcggcaatgc gcgatgcaag taatggcaaa caaattttag aacgaatttc
      181 aaatgaagct aatattgatg ttgagctgat tgatggcagc accgaagcca ggctgatcta
      241 tatgaaccat attgaagaaa acttagatcg taatagttca tatttatata ttgatgttgg
      301 tggcggcagt accgaactta cgttgttttc acaaaaccaa catatatttt caaaatcatt
      361 taacataggt actatacgca tgctgaatga acagattgaa aaggcttatt gggctgactt
      421 taaggattgg ataaaatcgg ccactaccaa attttatccg gttgtagcaa ttggcagtgg
      481 tggcaatatt aataagttgc ataaaatagc gcgtcttaag gatagtaaac cacttactta
      541 tacaaaactt gagatgttgt tcgaagaaat aaactcatac acctatcaag agcgtattga
      601 gttattggga cttaaacccg acagggctga tgttattata ccggcatgca aaatattttt
      661 atccgtaatg aaaaatgctt ctattgataa aatttttgtg cccgaaattg gtttaagcga
      721 tggtatcgtg catgaacttt atgaaagttt taaaactcaa aaaacataaa ttcagtgaaa
      781 gactttatta taacattcat tgtagcattt gttctattta aagtattgtc gagcattatg
      841 gatatgcgaa aacaacccaa taaaggaaac aatggtaata caacgcaacg ccatgtaggc
      901 gacataacca tagaagaaac acaagaagac aaaaacaaaa caaaccaaac gggttatact
      961 gattacgagg aaattaagta gttgcccatt tgcaaccacg atagattaat tcttacggct
     1021 gtttatattt tttatgtttt ctttgacatt aacttgtgta agcaccacac aaaaaccgaa
     1081 cacttattta tattaaaatt caatgctaaa aaaaacgcaa gctcttgaac atgtaattgc
     1141 aattgccatt ttcctatcac tgacatttgt atattttatg ccaatgtttc aaggtaaaac
     1201 cttaagtcag cacgatattg cacaatggga aggagcagcc aaagaaatta tagactttag
     1261 aaataccgaa catgccgagc ctctttggac taacagaatg tttggtggca tgcctgccta
     1321 tcaaataagc actttatatc catcaaacct gatagcgcat gttaatacat tcctaacttt
     1381 tggtttgcct tcaccggcat atttaatttt tatggggctg cttagttttt atattctcat
     1441 gttaaccctt aaagtagatt accgtttagc aattggcggt gccatcagct atgcttttgt
     1501 ttcgtttaat gtggtagtta ttcaagcagg tcataactca gaggcacatg caattgtact
     1561 attcccatta gtaattgccg gtgtgcttat ggtttttaat aaaaattatt gggctggcgg
     1621 tgctttaaca gctataggtt tggcattaca actgtatgcc aatcacttac aaattaccta
     1681 ttacacagct cttaccgtgc tttgtttggt tatagcccga agtatatatg caattaccaa
     1741 aaacgaaaca aaacacttac taaaaagtgc tttggtgtta agtattgcag ccactttagg
     1801 tgtattgccc aacattacca atttactggt aacaaatgaa tatggtaaag catcaacacg
     1861 cggcagtagt gagttaacag aaaaaaaatc ttcgacaggt ttagataaag attatgcatt
     1921 aaattggagt aatggcaagc gcgatgcggt aacattgtta atacccgatg caaccggtgg
     1981 cgcttcaacc aaaaaactcg atcacaaatc ggcaacttac aaggcattag aaagcaatgg
     2041 tgtattagat caggctgctg gtttgttaga tgcagcccca gtatattggg gaggtttgcc
     2101 aagcacttcg ggaccggtat atgcaggcgc tattattatt ctgttatgcg ttataggaat
     2161 gctaacttta aaagggcctg ataaatggtg gttactaatt gcaaccctgt tgtcggtttc
     2221 attatcatgg gggcgctact ttttgccgct taccgattta ttcttcaact actttccggc
     2281 ttacaacaaa ttcaggtcag tatcaatgat aatggtaatt gcttcgtttt gtttaccgct
     2341 actggcaata atgggcctgc aaggctttat taagcttact gatgcaagtg agcgaaagaa
     2401 aattttaatg cgtgcgctaa gttttacagg tggtttttgc gtgatagttt tactgggttc
     2461 ttacatgatt gattacagtg ccgagagcga caaatactat gcaaatgcgc cctggttaat
     2521 agctgcactt aaacaagacc gtgctgctat attaagagcc gataccctta ggtcattaac
     2581 ttttgttttg cttgcattcg gtttactatg gttttatata aaccagaaac ttaaattaca
     2641 cttactatat atgttgttaa gtgttttgtt tttatttgat atgtggcctg tagctaaacg
     2701 ttatttaaac aacgatagtt ttgctgaaaa gactgccgaa gaaacaccct ttacacccac
     2761 acaagccgac atgcaaattt tacaagataa gtcattaggc tatcgggtaa tgaatacaac
     2821 agttagtaca tttaacgatg caggtacctc ctactttcac aattcaatag gcggttatca
     2881 tggagccaaa ttaaaacgct atcaggaatt gattgagtac caaattggca aaggcaatca
     2941 ggcagtaata gatatgctaa acaccaagta ttttattttt caaaacaaac aaagcggctt
     3001 acccgaggca caaataaatc cgcaagcatg tggcaacgcc tggtttgtaa gcaatataaa
     3061 atttgttgtc aatgccgatg ccgaaatggc tgccttggat aaatttaatc ctaaaaccga
     3121 agtttttgtt gaccaacgtt ttaaaagtca ggtgggtagt attcctaatc aaactgatag
     3181 ttcagcatca atagtactta caagttataa acccaattac ataagttatt caagtcaaac
     3241 aaatacggaa caattcgcag tgctgtccga aatttactat gataaaggat ggaatgttta
     3301 tgtagatggc aaacccaccg attatgtaag atgtaattat gtgctacgcg gaatgaaagt
     3361 acctgccgga aatcataaaa tcgaatttaa atttgaaccg gcaacttatg ccatgggcga
     3421 aaaaatatcg ttagctggat cgctgctact ttttgccggt tgcctggctg tactttttat
     3481 ggagtttaaa aaaacaaaac aaagcccgga aacgctgcct gcaggtaaaa aaggcaaaaa
     3541 ttaaaattgc gttttagtat tgttaatgct taatgatatc cagttaataa atgtttttca
     3601 agcacacgtt tattgaaaga acttagctac ttaaataaat acttttataa ataccgtgga
     3661 cgtattgtat tgggtatttt atttgtggca ctcagcaaca ttttcagcat agtacaacct
     3721 attttaatca aacaaacact cgatgatgtt gctgctcaaa ttgctgccaa acacaataaa
     3781 acaacat
//
LOCUS       DAOMAA010000053         3714 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_17698_length_3714_cov_2.813337, whole genome shotgun sequence.
ACCESSION   DAOMAA010000053 DAOMAA010000000
VERSION     DAOMAA010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17698_length_3714_cov_2.813337"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..972)
                     /locus_tag="PLO59_01270"
     CDS             complement(151..972)
                     /locus_tag="PLO59_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99751.1"
                     /translation="MASACLLASCSNAPNPATDSTNDTTAAVAADTTPPNAYSEYLFQ
                     YTLLNLPPPMQILSEFSKSNMPVNVALLNTADNANKYKTATKQALNYGIYGMDLGYLV
                     VNNRILDIKKYYSTTQKLAADLNMSESFNAYTARFEKNSENKDSLSRLVDEALMATDN
                     YLRTNERLETASQALAGSWLECQYITVNLLKEQERTAENENLYQRVWEQRLYLDKITE
                     LLGTFKDNKEMTSVVNEYNQLLAIYKEPSDSKQITKDYLDKLAKKLNSVRNSIIN"
     gene            1299..>3714
                     /locus_tag="PLO59_01275"
     CDS             1299..>3714
                     /locus_tag="PLO59_01275"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQU99752.1"
                     /translation="MYISKRLWLTPLIKGSRLHILIVFMLAWAKLHAQQTQYYTDAEQ
                     YYKQGYELYLKEKYSAAIGQFDKALAQKNVSHISKVNAAFYSGVCSAELFNKNAEQKL
                     LSFINQYPNENKAQLAAWQLAKVYYREKQWKKSIPWFDKTDVAQLGNDEIPEYYFKSG
                     YAYFLTNDLNRASKNFHQITAVSSKYQTAANFYYAHIAYANGNYETALNSFQSLDKSE
                     AFAAVAPYYIAQIYFQQGRFEDVIKTGLPALQNGMPSNEIDLKRLVAEAYYQLGDFAN
                     AAKYLETYQKSTPSLNRNDMYQLGYSYYKTTAYDKAVTAFNKVINTTDTMAQNAYYHL
                     ADCFLKSGNKPAARNAFQFASKNNFDTKIQEDALFNYGKLSYETGATSAAINAFRDYI
                     KAYPKNAKSDQAFEYLAQAYLTTKDYKQALTALENIQTKSPNAKTAYQKVSYYRGVEL
                     FNDGDMPKAIGMFDKTIVNNNDAKLTAQAMYWKAEALYIQQNYDAAIKEYRIFLFAPA
                     ATHLDFYNNVNYNLGYCYFKKENYAESLTWFRKYVNEKDQTDVKRYNDAHIRIADAAF
                     MLRDYSTASANYNFAIKAGHSVSDYCYFQLGMLEGLQGNMTAKVAAMQQIENKYPKSL
                     YVDDAMYEKGNALLTKEDFSGALNQFEKVITQYPNSNYLSKARLKKGLIYFNQGNNKN
                     AIDAYKDVVTKHPNSNEANEALEGMKNAFIKEGKPDEFINFANNLPGPKIASGAQDTI
                     FYSAAEDAYQKEDYTKAAEAFSNYLKRFPEGAYKLHATFYKSKCDYFGTNYEAALQGF
                     ETILQQP"
BASE COUNT         1253 a          642 c          706 g         1113 t
ORIGIN      
        1 cggcattggc aataatggca aacagttgca ttaatgctct aagtaaacga tgactcataa
       61 gttgttgttt tgtgcagata ttgtactgcg tgaaaataaa aaagcccgtt aataatttta
      121 tcaacgggct ttttaaaaat ttgggtacgg ttaatttata atgctattac gtacggagtt
      181 tagttttttt gccagcttat ctaaatagtc ttttgtaatt tgtttcgagt cgcttggttc
      241 tttgtaaatg gctaatagtt gattgtattc atttaccact gaagtcattt ctttattgtc
      301 tttaaaagta ccaagtagtt cagtaatttt atctaaatac aaacgttgct cccacacacg
      361 ttggtataaa ttttcgtttt cggctgtacg ctcttgttct tttagcaggt taacggttat
      421 gtattggcac tctaaccaac taccggctaa tgcctggctg gcagtttcta agcgttcgtt
      481 ggtgcgcaaa taattatcag ttgccattaa agcttcgtca accaaacgtg atagcgaatc
      541 tttgttttcc gaattttttt cgaaacgtgc cgtataagcg ttaaacgatt cgctcatgtt
      601 taaatcggca gctaattttt gtgtggtact gtaatatttt tttatatcta aaatacggtt
      661 gtttactaca aggtatccta agtccatacc gtaaatacca taatttaaag cttgtttggt
      721 agccgttttg tacttgttag cattgtcggc cgtgtttaac aatgccacgt taacgggcat
      781 attgctttta ctaaactcac ttaatatttg catgggtggg ggcaggttaa gcaaggtgta
      841 ttgaaataag tattcgctat aagcgtttgg cggtgtggtg tcggcagcaa cagcggcagt
      901 tgtatcgttt gttgaatcgg tagcagggtt tggtgcattg ctgcacgatg ccaacaagca
      961 ggctgatgcc aaaagaaata agctgtattg ttttaatatc atgataaaga ttttggacaa
     1021 aaataagttt aattattaat tggcaagagg ttgcttgtaa agaaaagtcc aatttaaaag
     1081 gatgcttttt acatgcaatt taatagcggc tcaatttaaa gtgcaggctt aaaaatgtat
     1141 ctattatcaa cattacaggt ttcataaaat ctaaccggca gacaatataa aaatgctgct
     1201 tcacgttact acacaatatt tttgaagcca taacaaaacg ttacctatta gcttaatcaa
     1261 catttttaat tagcaagtgg ttgcgttaat ttatcatcat gtacatatca aaaaggttat
     1321 ggcttacacc tttgataaag ggtagccggt tacatattct aatagttttt atgcttgcat
     1381 gggcaaagct acatgcacag caaacacaat attataccga tgccgagcag tattacaagc
     1441 aaggttatga gttgtattta aaagagaaat acagtgcagc aataggccag tttgataagg
     1501 cattggcgca aaaaaatgta tcacatatca gtaaagttaa tgctgcattt tatagtggtg
     1561 tttgcagtgc tgaattgttt aataaaaatg ccgagcaaaa actgctttca tttattaacc
     1621 aatacccaaa cgaaaacaaa gcacaattag cagcctggca actggcaaaa gtttattatc
     1681 gcgaaaagca gtggaaaaaa agtattccct ggttcgataa aaccgatgtg gcgcagttag
     1741 gcaatgacga aataccggag tattacttta aaagtggtta tgcatatttt ttgaccaatg
     1801 atttaaaccg cgcatcaaaa aactttcatc agattacagc agtatcttcc aaatatcaaa
     1861 cggctgcaaa cttttattat gcgcatattg cttatgcaaa cggtaattat gaaacggctt
     1921 taaattcgtt tcaaagttta gataaaagcg aagcctttgc cgcagtagca ccttactaca
     1981 tagcgcaaat atattttcag caaggccggt ttgaagatgt gattaaaacc ggattgccgg
     2041 cattacaaaa tggcatgcca tcaaacgaaa ttgatttgaa acgtttagtt gccgaagcct
     2101 attaccaatt aggcgatttt gccaatgcag ctaaatatct tgaaacttat caaaaaagta
     2161 caccatcgtt aaaccgtaac gatatgtatc agttaggcta cagttattac aaaacaacag
     2221 cttatgataa ggctgtaacc gcttttaata aagtaattaa tacaacagat acaatggcac
     2281 aaaatgccta ctatcattta gccgattgtt ttttaaaatc aggtaataaa cctgcggcac
     2341 gtaatgcatt tcagtttgca tcaaaaaata atttcgatac taaaatacag gaagatgcac
     2401 tttttaatta tggtaaacta agttatgaaa caggtgcaac atcggctgct ataaatgcat
     2461 ttagagacta tataaaagcc tatcctaaaa acgcaaaaag cgatcaggct tttgaatatc
     2521 tggcccaggc ctatttaaca accaaagatt ataagcaggc attaacagca ttagaaaata
     2581 tacaaaccaa aagccccaat gcaaaaacgg catatcaaaa agtgtcatac taccgcggtg
     2641 ttgaattatt taatgatggc gatatgccta aggctattgg catgtttgat aaaactatag
     2701 taaacaataa cgatgcaaaa cttacggcac aggccatgta ttggaaagcc gaagcattat
     2761 atatacagca aaactatgac gcagccatta aagagtaccg tattttttta tttgcaccgg
     2821 cagcaaccca tctcgacttt tataacaatg taaattataa tttggggtac tgctatttta
     2881 aaaaagaaaa ttatgcagag tcgttaacct ggtttcgaaa gtacgtaaat gaaaaagacc
     2941 aaacggatgt aaaacgttat aacgatgccc atatacgtat tgccgatgct gcatttatgc
     3001 tgcgcgatta tagtacagca tctgccaact ataattttgc gataaaggca ggtcacagtg
     3061 tcagcgacta ttgttatttt cagttgggga tgttagaagg tttgcaaggt aatatgacag
     3121 ccaaagtagc agccatgcag caaattgaaa acaagtaccc caaatcgctt tatgtggatg
     3181 atgccatgta tgaaaaaggg aatgccctgc ttaccaaaga agattttagt ggtgcattaa
     3241 atcagtttga aaaagtaata acacaatatc ctaacagcaa ttatctaagc aaagcgcgat
     3301 taaaaaaagg gcttatttat tttaaccagg gcaacaataa aaatgcaatt gatgcctata
     3361 aggatgtagt taccaaacac cccaacagta acgaagccaa cgaagcattg gaaggcatga
     3421 aaaatgcttt tattaaagaa ggtaaaccgg atgagtttat aaactttgcc aataatttac
     3481 cggggccaaa aatagcttcg ggagcacagg ataccatatt ttacagcgct gccgaagatg
     3541 cttatcaaaa ggaagattat acaaaagcag cagaagcttt ttccaattat ctgaaacgtt
     3601 ttccggaagg cgcctataaa ctgcatgcaa ctttttacaa atccaagtgc gattattttg
     3661 gtacaaacta tgaagcggca ttgcagggat ttgaaacaat tttgcaacaa cctt
//
LOCUS       DAOMAA010000054         3705 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_17772_length_3705_cov_2.621918, whole genome shotgun sequence.
ACCESSION   DAOMAA010000054 DAOMAA010000000
VERSION     DAOMAA010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17772_length_3705_cov_2.621918"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            480..1166
                     /locus_tag="PLO59_01280"
     CDS             480..1166
                     /locus_tag="PLO59_01280"
                     /inference="COORDINATES: protein motif:HMM:NF027589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HQU99753.1"
                     /translation="MAYHAVITGDIIGSRLLSPVQKKQTMEVLKACFLMLQKRKDISF
                     EKGFEIFRGDSFQGVLRNPALALRVALIIRTCLMKQKITAHTKIKSNLKKPHQLTDTR
                     IGIGIGNINYKANKVIESDGEAFQLSGMLVDTMKSSGKRIMVGVPDKALSEVFQLQFT
                     LLDFIVNKWTEQQCEAIFEILIHNYNQQQLSQKLKISQPSIHQRLKISGWYAVAPFIE
                     YYETLKFSAA"
     gene            1166..1906
                     /locus_tag="PLO59_01285"
     CDS             1166..1906
                     /locus_tag="PLO59_01285"
                     /inference="COORDINATES: protein motif:HMM:NF023178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HQU99754.1"
                     /translation="MYNDTLLLFLKLLSAHLITDFILQKDNWIMQRNTLHYKAPSLYL
                     HTLITALFAYLFSALWQNWQIPLIVFVTHTLIDIGKSYCKPLFIYFFIDQMLHWFVLL
                     GCAWQYSTTTTDIIHTNEIMAMPNTFWVYAVAYLLVTFPMGYCIGALVKPFKEQLQQI
                     QNQSNDTLADAGKWIGIFERLLILTFVLANQFAAIGFLIAAKSILRFNNKDESFARKE
                     TEYVLVGTLISYALSIFTGLIANKLIQL"
     gene            2254..3618
                     /locus_tag="PLO59_01290"
     CDS             2254..3618
                     /locus_tag="PLO59_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002699423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQU99755.1"
                     /translation="MTHSKYNRRNFIRTGATAGLGLTLLPYAWATPQQKEKIKIGFIG
                     VGLRGQNHLELALKRTDVEVVAICDIQQVMLDTSIDMFKKANKTLPKQFKDNPYAYRD
                     LLKIKEIDAVIISTPWEWHYTMCMEAMQAGKYVGCEVITGITINEMHELVKTSEVTGK
                     PLMMLENVCYRRDVLAVLQMVRKNMFGEIIHLQGGYQHDLREVKFNDGKQPYGGGVQF
                     GATGFSEAQWRTAHSVLRNGDLYPTHGIGPLAMMININRGNRFVSLTSFASKARGLHN
                     YIVDKAGEQHPNAKVNFKLGDVVTTMIKCANEETILLTHDTSLPRPYSLGFRVQGAKG
                     IWMDLNNSIYLEGTSPQAHRWEPAKPYLENNDHPLWKKYGADATGAGHGGMDFFVLHA
                     FVESVKRQTQTPLDVYDAVTWSAITPLSEKSIAMGGQLVDFPDFTNGQWVNRKNKFAL
                     DDSY"
BASE COUNT         1242 a          695 c          673 g         1095 t
ORIGIN      
        1 actgaataga gcatctgact acggatcaga aggttggggg tttgaatccc tccgcgacca
       61 caaagcgaat cctgaatagg gttcgctttt ttattttagc tcaatctctg ctttgaattt
      121 agtatcgtta tcattttact ttaaatttaa gcattatgaa aaccgtactt cggctattga
      181 tgtgttttta aaacaatagc gtataatgca cttggcattg ttttaaatct ggcgatgcca
      241 tacaaatgga tattttaaaa ggcaaaattc cggctgcttc gtctattata ctgatgacaa
      301 caaaattatc aaaactaaaa taaccaatac cataaacgaa caatgagcaa tcataataca
      361 aacaccacta catgtgctga aacatttgct cgccaaccaa tattatacat aataaagcta
      421 taaatatagg tctatagacc tatatttgct tatatttgac cacgttcaaa aaaaactcta
      481 tggcatacca tgcagtaatt accggtgata taataggctc acgtttgttg tcgcctgttc
      541 aaaagaaaca aacaatggaa gtcttaaaag catgcttttt aatgcttcaa aaaagaaaag
      601 atatttcatt tgagaaaggg ttcgaaattt ttagaggcga cagctttcaa ggtgttttgc
      661 gcaacccggc tttggcatta cgcgttgcat taattatccg tacctgcttg atgaagcaaa
      721 agataacggc tcatactaaa ataaaatcca atttaaaaaa accacatcaa ttaaccgaca
      781 cacgaattgg aattggtatt ggtaatatca actacaaagc caataaagta attgagtcgg
      841 atggtgaagc ttttcaacta tcaggtatgt tggttgatac catgaaaagc agtggcaaac
      901 gaataatggt aggtgtcccc gataaagctt taagtgaagt atttcaatta caattcacat
      961 tgctcgattt tatagtaaac aaatggactg aacagcaatg tgaagctatt ttcgaaatac
     1021 tgattcataa ttataatcag cagcagttgt cgcaaaaatt aaaaatatca caaccatcca
     1081 ttcatcagcg tttaaaaata tcgggctggt atgctgttgc gccatttatt gaatattatg
     1141 aaacacttaa attttcggca gcataatgta taatgataca cttctgttat ttttaaaact
     1201 actttctgca catctaatca ccgattttat actgcaaaaa gataattgga taatgcaacg
     1261 taatacattg cattacaaag cgccatcctt atacctccat acattaataa cagcgctttt
     1321 tgcttactta tttagtgcat tatggcaaaa ttggcaaata cctttaattg tatttgtaac
     1381 ccatacccta attgacattg gcaagagcta ctgcaagcca ctatttatat acttttttat
     1441 tgaccaaatg ctgcactggt ttgtgttgtt aggctgtgcc tggcaataca gcactaccac
     1501 cactgatatt atacatacaa atgaaataat ggctatgcca aacacttttt gggtttatgc
     1561 cgttgcctac ttgcttgtta catttccgat gggttactgc attggcgctt tggttaagcc
     1621 atttaaagaa caattgcaac aaatccaaaa tcaaagcaac gatactttag ctgatgccgg
     1681 caaatggatt ggtatttttg aacgtctgct cattctcaca tttgttttgg ctaatcagtt
     1741 tgcggctatt ggttttctaa ttgcagcaaa atcaatttta cgctttaata acaaagacga
     1801 aagttttgcc cgtaaagaaa ccgagtatgt attggttggc acacttatca gctatgcatt
     1861 gtcaattttt acagggctga ttgccaacaa gttaattcaa ttataatatc caattaacgc
     1921 actggctact tttattcaaa caatagccac aaacttcaat taataattat agtatcagcg
     1981 acctataatt gtactttata gtatcagcga cctataatta cattttatag taccagcgac
     2041 ctataattac actttatagt atcagcgacc tataattgca ctttatagta ccagcgacct
     2101 ataattgcac tttatagtat cagcgaccta taattgtact ttatagtatc agcgacctat
     2161 aattacactt tatagtacca aaaacatata gttatacaaa ttgcatcatc aagttatatt
     2221 tacacaccct taataattaa ttatgcaaaa gccatgacac atagcaaata caacagacgc
     2281 aactttataa gaaccggtgc aacagcaggt ttaggcctaa cactattacc ctacgcctgg
     2341 gcaacaccgc agcaaaaaga aaaaataaaa ataggtttta ttggtgtagg cttacgcgga
     2401 caaaatcact tagaactggc attaaaacga accgatgttg aggtggttgc tatttgtgat
     2461 atacaacaag tgatgctgga tacaagcatt gatatgttta aaaaagctaa taaaacactg
     2521 cccaagcagt ttaaagataa tccgtatgca tatcgcgatc tgcttaaaat aaaagaaata
     2581 gatgcggtaa ttatatcaac accgtgggag tggcattaca ccatgtgtat ggaagccatg
     2641 caggcaggca agtacgttgg ttgtgaagta ataaccggta ttaccattaa cgaaatgcat
     2701 gagttggtaa aaacatcgga agtaacaggt aagccactta tgatgttaga aaacgtttgc
     2761 tacagacgcg atgtattggc tgtactacaa atggtacgta aaaatatgtt tggcgaaatt
     2821 attcacctcc agggtggtta ccaacacgat ttgcgtgagg tgaaatttaa tgatggtaaa
     2881 caaccctatg gtggcggtgt tcaatttgga gcaaccggtt tttcggaagc acagtggcgc
     2941 actgcacata gtgtacttcg aaatggcgat ttatatccaa cgcatggtat tggcccattg
     3001 gctatgatga ttaatataaa caggggtaac aggtttgttt cgcttacatc atttgcaagt
     3061 aaagctcgtg gcttacataa ttacattgtt gataaagccg gagaacaaca tcccaatgcc
     3121 aaagtaaatt ttaaactggg cgatgtggta actaccatga taaaatgcgc taatgaagaa
     3181 accatcctgt taacacatga tacgtcttta cctcgcccct actcgttagg ctttagggtg
     3241 cagggtgcaa agggtatttg gatggattta aacaatagca tttatttgga aggcacatca
     3301 ccacaagcac atcgctggga accggccaaa ccgtatttag aaaacaatga ccatcctctt
     3361 tggaaaaaat atggggctga tgcaacgggt gcaggccatg gcggtatgga tttttttgtg
     3421 ctgcatgcct ttgttgaatc cgtaaaaaga caaacacaaa cacctttaga tgtttatgat
     3481 gccgttacct ggagcgcaat aacgccattg agcgaaaaat cgatagcaat gggcggccaa
     3541 ttggttgatt tccctgactt tacaaacggc caatgggtta acagaaaaaa caaatttgcg
     3601 cttgacgact catattaacc taagccctat acgctattag caatagttta ttaaatccag
     3661 ccattaagtt tataataaag caaggcaaca tactcgcgca aacaa
//
LOCUS       DAOMAA010000055         3702 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_17800_length_3702_cov_3.196052, whole genome shotgun sequence.
ACCESSION   DAOMAA010000055 DAOMAA010000000
VERSION     DAOMAA010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17800_length_3702_cov_3.196052"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1608
                     /gene="groL"
                     /locus_tag="PLO59_01295"
     CDS             <1..1608
                     /gene="groL"
                     /locus_tag="PLO59_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682338.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HQU99756.1"
                     /translation="NLEARDALKRGVDALANAVKVTLGPKGRNVIIDKKYGSPSITKD
                     GVSVAKEIELKNPVENMGAQMLKEVASKTADVAGDGTTTATVLAQAMVTAGLKNVAAG
                     ANPMDLKRGIDKAVVAVVDALKKQSVEVGDDNQKIEQVATISANSDSYIGKLIADAMQ
                     KVGKEGVITVEEAKGTDTEVKIVEGMQFDRGYISPYFVTNADKMEADLENAYILLYDK
                     KISNMKELLPVLEKVVQTGRPLVIIAEDVDSEALATLVVNKIRGSLKIAAVKSPGFGD
                     RRKAMMEDIAILTGGTLIAEERGFKLENADLSYLGQAEKVTIDKDNTTIVNGVGSKED
                     ITARVNQIKAQVETTTSDYDREKLQERLAKLAGGVAVLYIGAATEVEMKEKKDRVDDA
                     LHATRAAVEEGIVPGGGVAYIRAIESIAKLKGDNEDENTGINIIRRSLEEPLRQIVAN
                     AGLEGSIIVDKVRNGKDDFGYNARTDKYEKMLAAGVIDPTKVTRVALENAASIAGMLL
                     TTECVLADIKEDKAPAMPTPDMGGMGY"
     gene            complement(1672..2532)
                     /locus_tag="PLO59_01300"
     CDS             complement(1672..2532)
                     /locus_tag="PLO59_01300"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQU99757.1"
                     /translation="MASFSHCIICNAPQLLAMPAYAHSYLVRCKACNFVFCSEIPTTQ
                     TLSDFYSRYPVSNSLSQLTAKRYDEWLDSFEPFRKLNTILDVGCGDGYLLERAILKGW
                     KVFGTEYTDRQVKQGIAKGINMHQGALDISNYAVNSFDVICSIEVIEHINNPMEELSK
                     FNYLLRQGGAVYVTTPNFNALSHDYFKAQWNIITYPEHLCYFTKSTLINAFLRNGFKC
                     SRFITTGISVKRYKQSVRNDRSIARSTDEQVRNKVEEKWYLRIVKNSINSLLTLFSKG
                     DTLKGLFIKQ"
     gene            complement(2581..3231)
                     /locus_tag="PLO59_01305"
     CDS             complement(2581..3231)
                     /locus_tag="PLO59_01305"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HQU99758.1"
                     /translation="MSGLLLLVLVTNPFIINQVLHAYEMKGYSEKQIVRPYDICIVMG
                     GSVRYFNGQTVRPVFGNGVDRLMQAVALYKAGKIKRILLSGGSGYVTLQHVKEADLLK
                     PVLLNFGIPDSVIYIENQSRNTFENAYMSKQLIADMPQIKNILIITSAYHVVRTKACF
                     EKLNLKADYFPVDALSGEISLTPDKILIPGADAVSNWVALLHEWLGLITYKMAGYC"
     gene            complement(3357..>3702)
                     /locus_tag="PLO59_01310"
     CDS             complement(3357..>3702)
                     /locus_tag="PLO59_01310"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQU99759.1"
                     /translation="IICDPDIVKIVVRNVLSNAIKFSHANNKILISTQIDAGNISLIV
                     TDYGLGMDKATLDSLFKPELTSKLGTQNEKGAGLGLTMCRDFMTQLGGAFNISSAVNK
                     GTTVFIKFKQGS"
BASE COUNT         1225 a          699 c          705 g         1073 t
ORIGIN      
        1 aacctcgaag cccgcgatgc attaaagcgc ggtgtagatg cattggcaaa tgcagtaaaa
       61 gttacattag gcccaaaagg acgtaacgta attattgata aaaaatatgg ctcaccatca
      121 atcaccaagg atggtgtttc ggttgccaaa gaaattgaac taaagaaccc ggttgaaaac
      181 atgggcgcgc aaatgcttaa agaagtagct tcaaaaacag ccgatgttgc cggtgatggt
      241 actacaacag ctactgtatt ggcgcaagct atggttactg caggtttaaa aaacgtagca
      301 gccggagcta atccaatgga tttaaaacgt ggtattgata aagccgttgt tgctgttgtt
      361 gatgcactta aaaaacaatc ggttgaagtg ggagacgaca atcagaaaat tgaacaggta
      421 gccaccattt cagcaaacag cgattcttat attggtaagt taattgccga tgccatgcaa
      481 aaagttggta aagaaggtgt aattacagtt gaagaagcta aaggtactga taccgaagtg
      541 aaaattgtag aaggtatgca attcgaccgt ggctacattt cgccatactt tgttaccaat
      601 gccgataaaa tggaagcaga tttagaaaac gcatacattt tattatacga taaaaaaata
      661 tcgaacatga aggaattact gcccgtactt gaaaaagtag ttcagacagg acgcccattg
      721 gtaattattg ccgaagatgt tgacagtgaa gcattggcaa ctttggtggt aaataaaatt
      781 cgtggttctt taaaaattgc tgctgtaaaa tcaccgggtt ttggcgatcg cagaaaagca
      841 atgatggaag atattgccat acttaccggt ggaactttaa ttgctgaaga acgtggtttt
      901 aaacttgaaa atgccgactt aagttactta ggccaagccg aaaaagttac tattgacaaa
      961 gataacacca ctatagtaaa cggtgtgggc agtaaggaag atataactgc acgtgttaat
     1021 cagattaagg cacaggttga aactacaacc agcgattacg atcgcgaaaa acttcaggag
     1081 cgtttggcaa aattagccgg tggtgttgca gtactttata ttggcgcagc taccgaagtt
     1141 gaaatgaaag aaaagaaaga ccgtgttgac gatgctttac atgcaacccg tgcagcagtt
     1201 gaagaaggta ttgtacccgg tggtggtgtt gcttacatac gtgccatcga atcgatagct
     1261 aaactaaaag gcgataacga agacgagaac acaggtatta atattatcag acgctcatta
     1321 gaagagccat tacgtcaaat agttgcaaat gccggcttag aaggcagtat tattgttgac
     1381 aaagtgcgta acggcaagga cgattttggt tacaatgcac gtaccgataa gtatgaaaaa
     1441 atgcttgcag ccggtgttat tgatccaact aaggttacgc gtgtagcatt agaaaatgca
     1501 gcatcaattg caggtatgtt gttaactacg gaatgtgttt tagctgatat taaagaagat
     1561 aaagctccgg ctatgccaac acctgatatg ggtggtatgg gctattaata aagcacactt
     1621 taataagctt accaaaaaaa ggctgtttcg aattttcgga acagcctctt tttattgctt
     1681 tataaataat ccttttaaag tatcaccctt gctaaataaa gtaagcaggc tatttatgct
     1741 gttttttaca atacgcaaat accatttttc ttcaacctta ttacgcactt gctcatcggt
     1801 gctgcgtgct atacttctat cgttacgcac actttgtttg taacgtttaa cggatatgcc
     1861 tgtagttata aatcggctgc atttaaatcc attacgtaaa aaagcgttga ttaacgtact
     1921 ttttgtaaaa tagcataagt gctcagggta tgtaataata ttccattgcg ctttaaaata
     1981 atcatgcgat aaagcattaa agtttggtgt ggttacataa acagccccgc cctgtctgag
     2041 caaataatta aacttactta actcttccat cggattattt atgtgttcaa taacttcaat
     2101 actgcatata acatcaaaac tattgactgc ataattgctg atatccaaag caccttgatg
     2161 catgttaatt cctttggcaa taccttgctt tacctgcctg tcggtgtatt ccgtaccaaa
     2221 taccttccac ccttttaaaa tagctctttc gagtaaatag ccatcgccac agccaacatc
     2281 taaaatggtg tttaattttc tgaatggttc aaaactatcc aaccactcat cataacgttt
     2341 tgctgttaat tggcttaggc tattacttac aggataacgg ctataaaagt cggatagggt
     2401 ttgcgtagtt ggaatttcag aacaaaagac aaaattgcaa gccttacatc taactaaata
     2461 actatgtgca taagcaggca ttgccagcaa ttgcggggca ttacaaataa tacaatgaga
     2521 aaatgaagcc attttggtta ataatgtgcc aataatacaa atttgaaatt ttgaaccgcc
     2581 tcaacaatat ccggccattt tgtaagtaat caatcccaac cattcatgca gcagcgctac
     2641 ccaattactt accgcatctg caccgggtat tagaattttg tctggggtta aacttatctc
     2701 accactaagt gcatcaaccg gaaaataatc tgctttcaaa ttaagttttt cgaaacaagc
     2761 tttggttcgt accacatgat aagccgaggt aataatcaat atgtttttga tttgtggcat
     2821 atcggctatt aattgtttcg acatgtacgc attttcaaaa gtattacgcg attggttttc
     2881 gatataaatt acactatcag gaattccaaa atttaatagc acaggcttta acaagtcagc
     2941 ttcttttaca tgctgcagcg taacataacc tgaaccgccc gacaataaaa tgcgttttat
     3001 ttttccggct ttgtataaag caactgcctg cattagccta tcaacaccat ttccaaaaac
     3061 aggtctaacg gtttgaccat tgaagtaccg caccgaacca cccattacga tacaaatatc
     3121 ataaggccta acaatttgtt tttcggaata gcctttcatc tcatatgcat gaagtacctg
     3181 gtttataata aatggattgg taactagcac aagtaacaat aagcccgaca caaaaaagca
     3241 acgtttaaac ctcttgtttt ttataaaaaa agctaagaac aataaagtaa ttacccataa
     3301 cagcgggtta attaaaaaac ttaaaatttt cgacagatag aaaaacattt aatagcctac
     3361 gaaccttgtt taaacttaat gaaaactgta gtgcctttgt ttactgcact gctaatatta
     3421 aacgcacctc ccaactgagt cataaaatcg cggcacatgg ttaaacccaa ccccgcaccc
     3481 ttttcgtttt gtgtacctaa tttcgaggtt aactcgggtt taaacaaact atctaaagtg
     3541 gctttgtcca tacctaagcc gtagtcggta actattaagc taatattacc tgcgtcaatt
     3601 tgggtgctta ttaaaatttt attattggca tgactaaact taatggcatt gcttaaaaca
     3661 tttcgtacca caatttttac tatatcaggg tcgcaaataa tt
//
LOCUS       DAOMAA010000056         3700 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_17820_length_3700_cov_2.813992, whole genome shotgun sequence.
ACCESSION   DAOMAA010000056 DAOMAA010000000
VERSION     DAOMAA010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17820_length_3700_cov_2.813992"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1263
                     /locus_tag="PLO59_01315"
     CDS             <1..1263
                     /locus_tag="PLO59_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99760.1"
                     /translation="VFDKNGLTVFDDEIKIPGGVFNNTYGIDDIVVLGNEAFALVEHL
                     DKAAGANTLTARKFESAGKVATAETALMKIPFEKIMNSGYNYAAVSPDNKTMAIVGEM
                     PFNKEQPCILKINLFDASLKNLHTQEITLPGENKKNKAFEVLVNNNGLVYIIKRSTTK
                     NGEIALTAYACDVTKNNTVKEFVIELAAPEYILSYKNGVADNGQLVVAGLSYKRATIS
                     ANETKTTGTFYFTNTGFEKPEMMVVPLTTPIDNLTARKLIFNGNTTFLAAEQYKAERE
                     TQPPGTTGFDYNYNYTHKNDYIIGFDNTGNRKFEMPLMKDFKARNFDLQHYANYFVVN
                     NKLTVFYNDETKKYIQNDYTYGYLLPTIVQITNDGLMQSPVVLRDKIRLPQQQILYPV
                     YGVQTSGNQINMLMINSNFMQLLTTKIE"
     gene            complement(1525..2976)
                     /locus_tag="PLO59_01320"
     CDS             complement(1525..2976)
                     /locus_tag="PLO59_01320"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HQU99761.1"
                     /translation="MKKILLALFAVFFLQQIHAQTEDKKWNIGVFGGATQYNGDLGQG
                     FYNFEQAFYGHGGLSVSRFLSPHFDVSIQGTMGEIGYVEEAGNRFRADLITGALHFRL
                     KLLKEEARIRPYFFAGAGIMVFGDEYTLPKKKTQHALPSAGAGFNIRLSDVVNLTIQE
                     TFMYSDDDLAVDGEKGNNNDSYLHHSVGLSFNLGSSKDADNDGVADKKDKCPGTPSGV
                     MVDKEGCPIDRDGDGIADFTDACPDVKGVASAKGCPDRDGDTVADDADACPDDAGLVD
                     LNGCPDRDGDRIIDKDDKCPDVKGLVEFAGCPDRDGDGIIDSEDLCPDVKGPAANKGC
                     PDRDGDGVLDKDDKCPDVKGIASNKGCPEVKAEVKEVFRKALQGIQFETGKDVIRKNS
                     YSILDQVAKIMVDNPEYKLLVNGHTDNVGKPESNMILSQKRADAVKKYLTAKGVDAGR
                     MTATGYGDTKPVADNKTTAGKKENRRVEFVVEF"
     gene            complement(3081..>3700)
                     /locus_tag="PLO59_01325"
     CDS             complement(3081..>3700)
                     /locus_tag="PLO59_01325"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HQU99762.1"
                     /translation="KLLAIERERNRISEDLHDDLGAGLSSIAMMTGVMRELITDEDSK
                     NTADEVSTEANELVARMREIIWSMNSKNDTVENMLTYINDYCHRYLAKNKIEIRMQMP
                     EYVPDINITSEARENTFLVIKETLHNAVKHAQSGKFSFQATLDTHWLTIKLNDHGKGF
                     DLNAINRFGNGMLNMEQRIHKISGKFLITSEPGQGTQTHIEIPIS"
BASE COUNT         1120 a          765 c          686 g         1129 t
ORIGIN      
        1 gtatttgaca aaaatggatt aaccgtattt gatgatgaaa ttaaaatacc cggtggcgtg
       61 tttaataaca cgtatggtat tgatgacatt gttgtgctgg gtaacgaagc gtttgccctt
      121 gttgaacatt tagataaagc tgccggtgcc aatacactaa ctgcacgcaa atttgaaagt
      181 gccggaaaag tagctactgc cgaaacagcg ttaatgaaaa ttccgttcga aaaaattatg
      241 aattcgggct ataactatgc tgctgtttcg cctgataata aaacgatggc tatagtaggc
      301 gaaatgcctt ttaataaaga gcaaccttgc attttaaaaa tcaatttatt tgatgcatca
      361 cttaaaaacc tgcatacaca agagataacc ctgccgggcg aaaacaaaaa gaataaagca
      421 tttgaagtat tggttaataa caatggcttg gtttacatta taaaacggag cactaccaaa
      481 aatggcgaaa ttgcactaac tgcctatgca tgcgatgtaa caaaaaacaa tacagtgaaa
      541 gaatttgtga ttgagcttgc agcacctgaa tacatattgt catataaaaa tggtgtagcc
      601 gataatggcc agttagttgt tgccggttta agttacaaac gtgctaccat atcggccaat
      661 gaaaccaaaa ccacaggcac tttttatttt accaataccg gtttcgaaaa acccgaaatg
      721 atggtagtgc cattaacaac acctatagat aaccttacag cacgtaaatt aatttttaat
      781 ggcaacacca cttttttagc agccgaacaa tataaagccg agcgcgaaac acaaccgccc
      841 ggcacaaccg gttttgatta caattataat tacacacata aaaacgatta cattattggc
      901 tttgataata cgggcaacag aaaatttgaa atgccattaa tgaaagattt taaagcacgc
      961 aatttcgacc tgcagcatta tgccaattac tttgttgtaa ataataagct tactgttttt
     1021 tataatgatg aaaccaaaaa atacattcaa aacgattata cctacggtta tttattgccc
     1081 accatcgtac aaataaccaa tgatggctta atgcaatcgc ccgttgtttt gcgcgataaa
     1141 atacgtttgc cccagcaaca aattttgtat ccggtttatg gtgtacaaac aagcggcaat
     1201 caaataaata tgttgatgat taacagtaac ttcatgcaac tgttaactac aaaaatcgag
     1261 taaacttaaa tctttgcttt agcataaata gccttgcagt ttaaagctgc aaggctattt
     1321 ttttgcggca tctttaaaag ttgccatgta taacaaattg taaaaacgca aactgatatt
     1381 catgtcttta aagcgtttaa acaaatatca ttagccctta tacgtaagtt aaaataataa
     1441 ggagaatgca ccggcaggta atagtgtttt tacaaaaaaa gggctgccta ttgggcagcc
     1501 cttttttgta actataatta attattaaaa ctcaaccaca aattcaaccc tgcggttttc
     1561 ttttttaccg gcagtggttt tattatcggc aacgggtttt gtatcgccat agccggtagc
     1621 cgtcatacgg cctgcatcaa cacctttggc agtaagatat tttttaactg catcggcacg
     1681 tttttgcgat aatatcatgt ttgattcggg ctttccaaca ttatcggtat gtccatttac
     1741 caataattta tactcaggat tatcaaccat aattttagca acctgatcta atatgctgta
     1801 agagtttttg cgtattacat ctttgccggt ttcaaattgt atgccttgca atgctttgcg
     1861 gaatacttct ttaacttcgg ctttaacctc gggacaacct ttgttgcttg caataccttt
     1921 aacatcaggg catttatcat ctttatctaa aacaccatcg ccatcacggt cggggcaacc
     1981 tttattggca gcagggcctt taacatcagg acataaatct tcgctgtcaa taattccatc
     2041 gccatcacgg tcggggcagc ctgcaaactc aaccaaacct ttaacatcag ggcatttatc
     2101 atctttatca attatgcggt cgccatcacg gtcggggcag ccgtttaagt caaccaaacc
     2161 tgcatcatca gggcatgcat cagcatcatc ggctacggta tcaccatcgc ggtcggggca
     2221 acctttggcc gatgcaacac cttttacatc cggacaagca tccgtaaagt cagctatgcc
     2281 atcaccatca cggtcaatgg ggcagccttc tttatcaacc attacaccac ttggtgtacc
     2341 cggacattta tcttttttat cggcaacgcc atcgttatcg gcatctttgc tgctacctaa
     2401 attaaacgac aaaccaacgc tgtggtgtaa gtacgaatcg ttgttatttc ctttttcgcc
     2461 atcaacagct aaatcatcat cgctatacat aaaagtttcc tgtatggtaa ggtttaccac
     2521 atccgacaaa cgaatgttga aaccagcacc ggccgatggc aatgcatgtt gtgttttctt
     2581 tttgggcaag gtgtattcat caccaaaaac cataataccg gctccggcaa aaaagtaggg
     2641 gcgtatgcgg gcttcttctt ttaaaagttt aagtctgaag tgcagggctc cggtaattaa
     2701 atcggctctg aaacggttac cagcttcttc aacataacca atttcgccca tggtaccttg
     2761 aatgcttaca tcaaaatgcg ggcttaaaaa gcgcgaaacc gataaaccac catggccata
     2821 aaaagcctgc tcgaaattgt aaaacccctg gcctaaatcg ccattatact gggttgcgcc
     2881 accaaataca ccgatgttcc attttttgtc ttcggtttgt gcatgaattt gctgcaaaaa
     2941 gaaaaccgca aaaagtgcga gtagaatttt tttcataata gatagatttt taattaagaa
     3001 acaatatgca acaatagtgc aaagtttgat ttgattaaaa ttatgattta taaattttca
     3061 ggttgcttat acacattgtt ttaactgatt ggtatttcaa tgtgtgtttg tgtgccctgc
     3121 ccgggttctg acgttattaa aaacttgcca cttattttgt gaatgcgttg ctccatatta
     3181 agcatgccat tgccaaaacg attaattgca tttaaatcaa accctttgcc gtggtcgtta
     3241 agtttaattg ttaaccaatg ggtatcgagt gttgcctgaa agctgaactt accactttgt
     3301 gcatgtttta cggcattgtg caaagtttct ttaattacta aaaaggtgtt ttcgcgtgct
     3361 tcgctggtga tattgatatc tggcacatat tcgggcattt gcattcgaat ctcaatttta
     3421 ttttttgcaa ggtagcggtg gcaataatcg tttatatagg tgagcatgtt ttctacggta
     3481 tcgtttttcg aattcatact ccagattatt tcgcgcatac gtgcaacaag ttcattcgct
     3541 tcggttgata cttcgtcagc cgtatttttt gaatcctcat cggtaataag ttcgcgcatt
     3601 acgccagtca tcatggctat tgatgaaagc cctgcaccca ggtcatcatg caggtcttcg
     3661 ctaatgcgat tgcgctcgcg ttcgatggct aacagttttt
//
LOCUS       DAOMAA010000057         3692 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_17895_length_3692_cov_2.451746, whole genome shotgun sequence.
ACCESSION   DAOMAA010000057 DAOMAA010000000
VERSION     DAOMAA010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3692
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17895_length_3692_cov_2.451746"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1445
                     /locus_tag="PLO59_01330"
     CDS             <1..1445
                     /locus_tag="PLO59_01330"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU99763.1"
                     /translation="VWAYRVDEYRNGVKIGSVTRDIQLVIFPGTDQLPTLSGINQYPG
                     SVTTICAGDSLMFDIFSADPDSGDSTFIAWHHFGQPNYTLTINGGQNEGASFAWATDS
                     TMISPNPYLLYVSVKDNACPYYGLQTYVYKIFVNQCITNDVWPGDANSDLVCNMYDIL
                     PIGLTYGNTGPTRTGASLNWLAQPALAWNNTIASGVNNKHADCDGNGVIDSLDLIAIT
                     LNYGSTHNKMGNLVEPHINGLPDLKVTYSAQQVAPGAPVVATIELGNASTPLNAVYGI
                     AYTLNYSVNMFQLGTAGFSNTNSFVGNTNNALRLTEGVIDYGSITTGLVRIDHNNASG
                     YGEIGKFTFTANPLIIGDVTFNMTFSNVRLVMNDGTQVPVNVINNNSFTISQLLGVAD
                     LSNQIQHLSIRPNQTKDIATVSYQLGTTTNVTLEIMDMLGNIVQTEKYGNVSAGVQQV
                     VLNIAGLNQGAYLVRVSSANGIATQRLIKL"
     gene            1783..2238
                     /locus_tag="PLO59_01335"
     CDS             1783..2238
                     /locus_tag="PLO59_01335"
                     /inference="COORDINATES: protein motif:HMM:NF019238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HQU99764.1"
                     /translation="MNEKIKYLTQTFIDQLQTLKSDDTGQWGVMKAQHMVEHMSYSVK
                     VAHGAIPVTLVTPAAFLEKTRSFMLSDKPFKENTKNTMLPDEPLPLMFDNMQQAIEDL
                     QLQIQLFINDFNVEGKTRLNPFFGELNFVQWLHLLDKHAKHHARQFNLL"
     gene            complement(2299..3096)
                     /locus_tag="PLO59_01340"
     CDS             complement(2299..3096)
                     /locus_tag="PLO59_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99765.1"
                     /translation="MKNLNFIAALLLATTALTSCQKENTSTPFYGSNNHGQSAKESDY
                     LYLKDVINTKGEVCQSFVYNDEKKLIQINMPVDVSQSFIYDEKGNLESATTLDGKTSQ
                     VTEQLVFLYNDDASLPHLMNTYINEGGALKLMAVTKIEWTAEGLKKHEHTTLEATQET
                     LETLFEYSDDNASIITKFKDGNLASTKVIKQYDDSENPYAKIKTLRLLPGFEWFKNNT
                     VFSFVESNGNVENFTNEITYDEFGLPRIIKTVMDNGEKTSMQFTYDK"
BASE COUNT         1126 a          616 c          693 g         1257 t
ORIGIN      
        1 atgtttgggc atatcgtgtt gatgagtata gaaacggtgt taaaattggt agcgttacac
       61 gtgatataca attggtaata tttccgggta ccgatcaatt gcctacatta tcaggtataa
      121 accaataccc gggttcggta acaactattt gcgcaggcga ctcattaatg tttgatattt
      181 ttagtgccga tcctgacagt ggagatagta cttttattgc atggcaccac tttggccaac
      241 ctaactacac acttaccatt aatggaggcc aaaatgaagg tgcaagtttt gcatgggcaa
      301 ccgattcaac aatgattagc cctaaccctt acctcttgta cgtatcagta aaagacaatg
      361 cttgtcctta ttacggattg caaacttacg tttacaagat ttttgtaaac caatgtatta
      421 cgaatgatgt atggccgggc gatgctaact ctgacttagt gtgtaatatg tacgatatat
      481 tgcccattgg cttaacatac ggtaataccg gtccgacccg taccggtgcc tcattaaatt
      541 ggttggcgca acctgcttta gcctggaata acactattgc cagtggcgta aataacaagc
      601 atgccgattg tgatggcaat ggtgttattg actcattaga tttaatagcc ataactttaa
      661 actatggcag tacccataat aaaatgggta atctagttga gcctcatata aatgggttgc
      721 ccgatttaaa agttacctat tcggcacagc aagttgcacc gggtgcgcct gttgttgcaa
      781 ctatcgaatt aggtaatgca tcaacacctt taaatgctgt ttatggtatt gcttatacat
      841 taaactacag cgtaaatatg tttcaactgg gcacagcagg ttttagtaat acaaattcat
      901 ttgtgggtaa tacaaacaat gcattgcgcc ttactgaagg tgtaattgac tacggtagta
      961 ttacaacagg tttggttcgt attgaccaca ataatgcaag cggctatggc gaaattggta
     1021 agtttacatt tacagccaat cctttaatta ttggcgatgt aacatttaat atgactttta
     1081 gtaatgtaag gttggtaatg aatgatggta cacaggtgcc tgttaatgtg ataaacaata
     1141 actcgtttac catttcacaa ttattaggcg tagctgattt aagtaatcag atacaacatt
     1201 taagcatacg ccccaatcaa acaaaagata tagcaactgt aagttatcaa ttgggtacta
     1261 caactaacgt aaccttagaa ataatggata tgttaggtaa tatagttcaa accgaaaaat
     1321 atggaaatgt tagtgcaggt gtacagcaag tggttttaaa tattgccggt ttaaaccaag
     1381 gtgcttactt ggttagagtt agttcagcaa atggcattgc tacgcaacgt ttgataaaat
     1441 tgtaaatgct taaaataaaa aacccgttta aagtttaggc tttaaacggg tttttttgtg
     1501 cgatactttt attaatccaa gcgcataaaa aagccgttga ccttaaaagg caacggcttt
     1561 tattattttt tgcagcttag cttaaatagt gcaccttaaa aggtaaaaaa tagaagataa
     1621 tataagtgct atccacaaca tggccactac gctagtaccg ctgtgaccaa aataagtttc
     1681 cataaattta cgcatggctg tatttgtttg agagtttttt gttggtgtgg gcaaaagtaa
     1741 aaattatgtt ttaggttgca agccattaaa aaattcaaat ctatgaacga aaaaattaaa
     1801 tatctgacgc aaacttttat tgatcaattg caaaccttaa aatcagatga caccggccaa
     1861 tggggcgtta tgaaagcaca acacatggtt gaacacatga gttactcggt taaggttgcc
     1921 catggtgcta tacccgttac attggtaaca cctgctgcgt ttttagaaaa aacgcgcagc
     1981 tttatgctta gcgataagcc ttttaaagaa aacaccaaaa ataccatgtt acctgacgaa
     2041 ccgctgcctt tgatgtttga taatatgcaa caggctattg aagatctgca gttacaaata
     2101 caacttttca taaacgattt taatgttgaa ggtaaaaccc gactaaatcc tttttttggt
     2161 gaattgaatt ttgtgcagtg gttgcatttg ttagacaagc atgccaaaca tcatgcacgt
     2221 caatttaatc tattataaaa aaagcccctt actttaggta agaggctttt ttagttttca
     2281 atttatccgc agtggttgtt acttgtcata agtaaattgc atacttgttt tttcgccatt
     2341 atccataacg gtttttatta ttcgaggcaa accaaattca tcataagtaa tttcattggt
     2401 aaaattttct acgtttccgt ttgactcaac aaagctaaac acggtattgt ttttaaacca
     2461 ctcgaagccg ggtaacaagc gaagtgtttt tattttggca taagggtttt cgctgtcgtc
     2521 atattgcttt ataactttgg ttgatgcgag attgccatct ttaaattttg taatgatgct
     2581 tgcattatca tcgctatatt caaacaaggt ttctaaagtt tcttgtgttg cttctaaagt
     2641 tgtatgctca tgttttttca agccttctgc tgtccattca atcttggtta ctgccattag
     2701 ttttaaggca ccgccttcat taatgtaagt gttcatcaaa tgtggcaaag atgcatcatc
     2761 attgtataag aaaaccaact gctcggttac ctggctggtt ttgccgtcta atgtggttgc
     2821 gctttcaaga tttccttttt cgtcatagat aaatgattgc gatacgtcaa ccggcatgtt
     2881 aatttgaatt aatttctttt cgtcattgta cacaaacgat tggcatactt cgcccttagt
     2941 gtttattaca tctttcaaat aaaggtaatc gctttctttt gccgactgac cgtggttgtt
     3001 gctgccataa aatggtgtac tggtgttttc tttttgacaa gaagttaatg ctgtagttgc
     3061 taacaataat gctgcaataa aatttagatt tttcatgttg tgattttttt agatggttaa
     3121 tgattaaatg atggttaatt aatgagtcaa agatgcagtg taacttaagc caaatcaagc
     3181 gtagaaaccc gcattttaca gcgtgtttac ccttgttttt tagtattaaa atataaacca
     3241 cattgtgtat ttagaccggt ttggtttagc ctaatcgttt tggttagaaa tatgtttgct
     3301 agtttgataa acagctttaa gaaatgagtt ggttgttttg ataattacct taacaccaac
     3361 cttttttgcc atgcttattg caataaaaaa attgacggta ttgatatcat ttataaaagc
     3421 aagactatct ttttattgta aatactatta acaggtacac tgattttatt tttaaaatta
     3481 ctaaggcaat gtatttttat aaaggcgcca atatgagttg catcaactta taaaaaacaa
     3541 aaaggccata agcaattttg cttatggcct ttttgttttt gtgtttacct tataatttta
     3601 tatatgtaaa tccttgttta gtggttaacg ggctgccgtt tatcgaagtt tcaattgatg
     3661 taggtagttt ttgattattg taagtaatgg ta
//
LOCUS       DAOMAA010000058         3679 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_18007_length_3679_cov_3.291391, whole genome shotgun sequence.
ACCESSION   DAOMAA010000058 DAOMAA010000000
VERSION     DAOMAA010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18007_length_3679_cov_3.291391"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1598
                     /locus_tag="PLO59_01345"
     CDS             <1..1598
                     /locus_tag="PLO59_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99766.1"
                     /translation="YGLMITEPASTNIDNNVFAYFLTNNAKASLKLIGATVAKNASET
                     YRNHQIITLPDNLLPQAFGSTFSYLKNTIATHIGSFVFIANNKEQLNVIIDAYENKNN
                     WSALPQSKEMLSQFAAVGNYFCYANVPRCNYLLQGKANPGASKWLTTNKATTALFDAF
                     AFCIKPSNNQLETTAYLHTLNKPTSQANVLWSQTLEAPALFAPQLVMANDSITYIAIQ
                     DTLNQLYLIDNTGLLKWKQTLPEKITGNIHTIDAYKNGSRYLVFATAQKWYLLDLKGM
                     PVSNFPIKLPAATNVGLSIFDLDLTGDFRIYIPCTNGIVYGYNTNGKPLNNFNAAYSA
                     HILQPLQYIGVNQKLLLGFTNNQHVVGFERNGKIALDIALPAAVHTPCVVNNLPNGFQ
                     FIDSVGTEISIDSTGNMVPLTVDYSVSFNLKQNDSTTIQFKIFNNYCTAELNNKTLFN
                     YNFSESVAPQLGIGLEGNQPVYLLQNYNQSKTYALHANGKLINGFPVAGMYKSHITDL
                     NGDQQLRLVLLTAANVLSVYSLK"
     gene            1686..3455
                     /locus_tag="PLO59_01350"
     CDS             1686..3455
                     /locus_tag="PLO59_01350"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008505997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HQU99767.1"
                     /translation="MNPFVVSARKYRPATFDSVVGQSHITVTLKNAIKNNHLAQAFLF
                     CGPRGVGKTTCARILAKTINCLNINDNTEACNTCESCDAFNKQASFNIYELDAASNNS
                     VEDIRALVEQVRYAPQYGKYKIYIIDEVHMLSSNAFNAFLKTLEEPPAHAIFILATTE
                     KHKIIPTILSRCQIFDFNRIKIDDIAMQLQSIALAEAVQAEPEGLHIIAQKADGAMRD
                     ALSMFDQIVSFAGNTVTYKDVINNLNVLDYDYYFKITDQLLNNDYKALLLTYDELINE
                     GFDSHHFINGLSGHFRDLLVCQDAVTLSLLEVADKIKAKYQTQASGIEADLLMAWIEI
                     CNKTDVEYRLAKNTRLHVELALLKMCRLKKKQHSDLSFVEVLEMVSLNTATSVKTNNP
                     GSTTQNVVSSAIAPVVNTQPMQQNYTAANTAFALSEPKQPLQTISKSAISLTPSLKNM
                     GKPKVDETAIEEKKPTLNQSFSISELHQAISDFAQLMLQQNKRNLAYTLQTHIATLVN
                     KAEVVFELQNAAQAEDITQQKVAMLQHLKSALKNDFIVITTHVISNTDTGNNLYTPAE
                     KFAEMARKNPALLAFKSKFDFDL"
     gene            complement(3472..>3679)
                     /locus_tag="PLO59_01355"
     CDS             complement(3472..>3679)
                     /locus_tag="PLO59_01355"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HQU99768.1"
                     /translation="LQIIEQLNASGAYASKIVTEVTPLDVFYKAENYHQNYYRLNSSE
                     GYCRMVIQPKLEKFKKVFPDKLIK"
BASE COUNT         1266 a          698 c          620 g         1095 t
ORIGIN      
        1 agtatggttt gatgattacc gaacctgcaa gcactaatat tgacaacaat gtgtttgcct
       61 atttcttaac caataatgca aaggcatcgc ttaaattaat tggcgctaca gtagctaaaa
      121 atgcaagcga aacctatcgc aatcatcaaa taataacatt gcccgataat ttattgccac
      181 aagcatttgg cagcacattc agttatttaa aaaataccat tgccacacat attggctcct
      241 ttgtatttat tgcaaacaac aaggaacaac tgaatgtaat tattgatgct tatgaaaaca
      301 aaaacaactg gagtgcttta cctcaaagta aagaaatgct aagtcagttt gcagccgtag
      361 gcaactactt ttgttatgct aatgtacctc gttgtaacta tttacttcaa ggcaaagcta
      421 accccggagc atcaaaatgg ctaaccacta acaaagctac aactgcattg tttgatgcct
      481 ttgcattttg tattaagcca tctaacaatc aactggaaac aaccgcttat ttgcacacat
      541 taaacaagcc tacttcgcaa gccaatgtat tgtggtcgca aacattagaa gctccggcac
      601 tttttgctcc ccaattggtt atggctaacg atagcattac ctacattgcc attcaggata
      661 cgctcaatca attatacttg attgataaca ccggcttatt aaaatggaaa caaaccctgc
      721 ccgaaaaaat taccggtaat attcacacaa ttgatgccta taaaaatggc agtcgttatt
      781 tagtatttgc tacagcacaa aaatggtatt tgttagattt gaaaggtatg cccgtatcaa
      841 actttccaat taaattacct gctgcaacaa atgtagggct aagcattttt gatttagatt
      901 taacaggtga ttttcgcatc tacatacctt gtacaaatgg cattgtgtac ggctataata
      961 ccaatggcaa accactaaac aattttaatg ctgcatatag cgctcacata ttacagccat
     1021 tgcaatacat cggtgtaaat caaaaattat tattgggctt tacaaacaat caacacgttg
     1081 ttggattcga acgtaatggt aaaatagcgt tagatatagc attgcctgct gcggtgcata
     1141 cgccttgtgt agtaaataat ttgcccaacg gctttcaatt tattgatagt gttggtactg
     1201 aaatttctat tgattcgaca ggtaatatgg tgccgctgac tgttgactac agtgtatcat
     1261 ttaatttaaa acaaaatgac tcaacaacca ttcaatttaa aattttcaat aattattgta
     1321 ctgctgagtt aaacaataaa acattattta attacaattt ttctgaatca gtagcgccac
     1381 agcttggtat tggattagaa ggcaatcagc ccgtttacct actgcagaat tataatcaat
     1441 caaaaacgta tgcattgcat gccaatggta aacttataaa tggttttccg gtggccggca
     1501 tgtacaaatc gcacattact gatttaaatg gcgaccagca attgcgtttg gttttattaa
     1561 cagctgctaa cgtactgagc gtttatagcc tgaaataaca agctttaaaa attttgtttt
     1621 acacaattag cttaggcttc aatttaaacc tataaagttt aaatgctaaa attacatttg
     1681 caggtatgaa tccatttgtt gtatcagcac gcaaatacag gccagccaca ttcgatagcg
     1741 ttgtaggcca aagtcatata accgttacct taaaaaatgc gattaaaaac aatcatttag
     1801 cacaagcatt tttattttgt ggtccacgtg gtgttggcaa aacaacctgt gcccgcatac
     1861 ttgctaaaac cattaactgc cttaatatca acgataatac cgaagcttgc aatacatgcg
     1921 aatcgtgtga tgcatttaac aaacaagcat cttttaatat ttacgaatta gatgcagcat
     1981 ccaataacag tgttgaagat ataagagctt tggttgaaca agtgcgctat gcaccgcaat
     2041 atggtaagta taaaatttat attattgacg aggttcacat gctttcgagt aatgccttta
     2101 atgcatttct aaaaacactg gaagaaccgc ctgcacatgc catttttatt ttagcaacta
     2161 ccgaaaagca taaaataatt ccaaccattt tatcacgttg tcagattttt gattttaatc
     2221 gaattaaaat tgatgacatt gccatgcaat tgcaaagcat tgcactagcc gaagccgtac
     2281 aagccgaacc cgaaggcctg catataattg cacaaaaagc tgatggcgcc atgcgtgatg
     2341 cacttagtat gtttgatcag atagtaagtt ttgcaggtaa tacggttaca tataaagatg
     2401 taataaataa tctgaatgta ttggattacg attattactt taagataacc gaccaacttt
     2461 taaataacga ctacaaagca ttgctactta cttatgatga attaattaat gaaggatttg
     2521 acagccatca ttttataaat ggcttaagtg gccatttcag agatttactg gtatgtcagg
     2581 atgccgtaac cctatcattg ttagaagttg ccgataaaat taaagccaag tatcaaacac
     2641 aagcttcagg tattgaagcc gacttattaa tggcctggat tgaaatttgt aataaaaccg
     2701 atgtggaata ccggcttgca aaaaacacca gactgcatgt tgaactggca ctattaaaaa
     2761 tgtgtcggtt aaaaaaaaaa cagcatagcg atttaagttt tgttgaagtt ttagaaatgg
     2821 ttagtttaaa cactgccact tcggttaaaa ccaacaaccc gggcagcact acacaaaatg
     2881 ttgtatcaag tgccattgca ccagttgtaa atacacaacc gatgcaacaa aactacactg
     2941 cagcaaatac ggcatttgca ttaagcgaac ccaaacagcc actacaaaca ataagcaaaa
     3001 gtgcaattag cttaacgccc tcattaaaaa acatgggtaa acccaaggtt gatgaaacgg
     3061 ctattgaaga aaaaaaaccc actttaaatc aaagtttttc gataagtgaa ttacatcagg
     3121 ctataagcga ttttgcccaa ctgatgttac aacagaacaa gcgtaactta gcctacacat
     3181 tgcaaacaca tattgctacg ctggtaaata aagcagaagt tgtttttgaa ctacaaaatg
     3241 cagctcaggc cgaagatatt acgcagcaaa aagtagccat gttgcaacat ttaaaatcag
     3301 ctttaaaaaa cgattttatt gttataacca cccatgttat ttcgaacacc gatacgggaa
     3361 ataatttata tacccctgca gaaaaatttg ccgaaatggc ccgtaaaaat cctgctttgc
     3421 ttgcctttaa atcgaaattt gatttcgatt tataaataaa acagggccac tttattttat
     3481 cagtttatca ggaaatacct ttttaaattt ttcgagtttg ggttgaataa ccatgcggca
     3541 gtaaccctca ctactattta agcggtaata gttttggtga taattttcgg ctttataaaa
     3601 tacatctaac ggagtaacct cggtaacaat tttgcttgca taagcacccg aagcatttaa
     3661 ttgttcaata atttgtaat
//
LOCUS       DAOMAA010000059         3679 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_18011_length_3679_cov_2.731512, whole genome shotgun sequence.
ACCESSION   DAOMAA010000059 DAOMAA010000000
VERSION     DAOMAA010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18011_length_3679_cov_2.731512"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="PLO59_01360"
     CDS             <1..653
                     /locus_tag="PLO59_01360"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HQU99769.1"
                     /translation="RVFNLQEIALGKTVADLAPIRTQVASAKSMEDMAAWLKDKKIEF
                     VANAGIRTIKPNPNINEQLYDSVDLKTTQYESTLQCNTYFPLGKRHVINVGLQAGGIF
                     SDLIFENEVFRIGGLQSLRGFDEQSILASQYAIGKFEYRFILDAGSYLQAFFNTAYIR
                     QTISNTITDTPYGFGAGISFETRLGFFSFNYALGKQFDNPIYVRAAKIHFGIINYF"
     gene            659..1891
                     /locus_tag="PLO59_01365"
     CDS             659..1891
                     /locus_tag="PLO59_01365"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HQU99770.1"
                     /translation="MTPYRFIKNNVILVLIIYLLNLTANAQHSTVPITDNQLTLQPQF
                     CLYQYSTDSLMLWLQLNNDGLQYENDSMPVALVNVNLFVLEKAQSKKYLDQDAIHFKV
                     IATDENYTTVKMFFAKPAIQQFVIKLEVVDVQAKNKTVQFRQFNQTGLITEISMLATQ
                     NNGLPYFGNWVKTTDSVNVFASTVPMQKAWMRYFKFNNELPLPPHVTVSQNTFSYIAD
                     SAVEVNYTQQTFVLNKPGLYHFQADTMQKNGFTLMSFDSDFPNINRTQDMLPPLRYLT
                     TNAEFEKMQQAENKTTAMQEFWTKCAGDKMKSRKLIEAYYYNVQYANLNFTSYIAGWQ
                     TDRGLIYIIMGPPDAIDYNEKTIVWSYNLNFGQNLFFEFIKMNNPFSQNDYMLQRAAD
                     FETPWYQAVNKWRQGQTR"
     gene            1895..2665
                     /gene="rlmB"
                     /locus_tag="PLO59_01370"
     CDS             1895..2665
                     /gene="rlmB"
                     /locus_tag="PLO59_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015807879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HQU99771.1"
                     /translation="MYKHKPSKAIANKDFIFGIHAIIEAIDAGKQPDKILIQVGISNP
                     LIQQLKQKLKQNDWVYQQVPIEKLNSITTKNHQGAIAFITEIEFENLSNIIARVFENG
                     RHPFILILDRVTDVRNFGAIARTAVCAGVDALVIPSRGAAQINADAVKTSAGALHKLA
                     VCKVNDLKQEIQFLKNSGLQIVACTEKTNQLHFDVAYTQPTAIIMGAEDTGISPEFLK
                     LSDVKVRIPMSGLIDSLNVSVSAAIVMYEAVRQRNTLK"
     gene            complement(2688..>3679)
                     /locus_tag="PLO59_01375"
     CDS             complement(2688..>3679)
                     /locus_tag="PLO59_01375"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQU99772.1"
                     /translation="HTSINYYQAQKFKQQFTYPATVSISAMASQISTLKFGDKKTNPN
                     IFIMGADDGYLNVSGLSLETGRNFSPTDLTLGASVIILGSEVANTLFKSKPAINQQIF
                     VGSYKYVVIGVLKEKGSSFGMSPDKNCIIPLLNLKNRYASNSTTYTTTVNVSDIRFLD
                     QAIGEATGLMRSIRRDDILSDNSFEITKSDGLANQVIESTSDIRYAAIAIAAITLLGA
                     SIGLMNIMLVSVTERTREIGIRKSMGATAANIRHQFLAESIMICQLGGLLGVFLGIGI
                     GNTLSLFIGSPFLLPLNWILLAVALCFTVGIVSGYYPAAKAAKLDPIEALRYE"
BASE COUNT         1203 a          693 c          675 g         1108 t
ORIGIN      
        1 gccgcgtttt taacctgcaa gaaattgcct tgggcaaaac agtggccgac ctggctccca
       61 tacgtaccca ggtcgctagt gccaaatcca tggaagacat ggccgcctgg ctcaaggaca
      121 agaaaattga gtttgtagcc aatgccggta tccgaaccat aaaacctaat ccaaacatta
      181 atgagcagtt gtatgatagt gttgatttaa aaacaacaca atatgaaagt acattacaat
      241 gtaacaccta tttcccattg ggtaaacgcc atgttattaa tgttggcctg caagccggtg
      301 gtatttttag cgatttgatt ttcgaaaacg aggtttttcg tataggcggt ttacaaagtt
      361 tacgtggctt tgatgaacaa tcaatattag catcgcaata tgccatcggt aaattcgaat
      421 atagatttat tttagatgca ggctcttacc tgcaagcgtt ttttaatacg gcttacatca
      481 ggcaaactat tagtaacacc ataaccgata caccctatgg ttttggtgcc ggtatatcat
      541 tcgaaaccag attgggtttt ttttcgttta actatgcatt aggcaagcag ttcgataatc
      601 cgatatatgt tagagcggct aaaattcact ttggaataat aaattacttt taaaagcaat
      661 gactccttac cgatttataa aaaataatgt tatcctggtt ttaataattt accttttaaa
      721 tttaacagct aatgcgcaac atagtacggt gcctattacc gataatcaat taacgttaca
      781 gccgcagttt tgtttgtacc aatatagcac tgatagttta atgctatggc tacagttaaa
      841 taacgatggg ttgcaatatg aaaatgattc gatgccggta gctctggtta atgtaaactt
      901 gtttgtgctc gaaaaagcgc aatcgaaaaa atatttagat caggatgcta ttcattttaa
      961 agttattgct actgacgaaa attatacaac tgtaaaaatg ttttttgcta aaccggcaat
     1021 acagcaattt gtgattaaac tcgaagtagt tgatgtacaa gctaaaaata aaacagtaca
     1081 attcaggcag tttaatcaaa ccggtttgat aactgaaatc agtatgcttg caactcaaaa
     1141 caatggcctg ccttattttg gcaattgggt taaaactacc gatagtgtaa acgtatttgc
     1201 atcaacagta cccatgcaaa aagcatggat gcgctatttt aaatttaata acgaattgcc
     1261 attaccacca catgtaacag ttagtcaaaa cacatttagt tatatagccg atagcgctgt
     1321 tgaagtaaat tatactcagc aaacatttgt tttaaataaa ccgggattat atcactttca
     1381 ggcagatacg atgcaaaaaa atggctttac gctgatgagt ttcgacagtg attttcccaa
     1441 tatcaaccgc acccaggata tgttaccgcc attacgttat ctgacaacca atgccgaatt
     1501 tgaaaagatg caacaggccg aaaataaaac tacagccatg caggagtttt ggactaaatg
     1561 tgcgggtgat aaaatgaaaa gccgcaaatt aattgaagcg tattattata atgtgcagta
     1621 cgctaattta aattttacca gttatatagc aggctggcaa accgaccgtg gcttgatata
     1681 tattattatg ggtccaccgg atgcaattga ttataatgaa aaaacaattg tttggagtta
     1741 taatttaaat ttcgggcaaa atttattttt cgaatttatt aaaatgaaca acccatttag
     1801 ccaaaacgac tatatgttgc agcgtgcagc tgattttgaa acaccatggt atcaggcagt
     1861 aaacaaatgg cgtcaagggc aaacccgtta aatcatgtat aagcataaac catccaaagc
     1921 catagccaat aaagatttta ttttcggcat acacgcaatt atcgaagcaa ttgatgcagg
     1981 caaacaaccc gataaaattt taattcaggt gggtatttca aacccgttaa tacaacagtt
     2041 aaaacaaaag ctaaaacaaa acgattgggt atatcaacaa gtgcccatcg aaaaacttaa
     2101 tagcattaca actaaaaacc atcagggcgc tattgcattt atcaccgaaa ttgaattcga
     2161 aaatctttcg aatataattg ccagggtttt tgaaaatggt cgccacccat ttattttaat
     2221 attagaccgg gttaccgatg tacgtaactt tggagccatt gcccgtacgg cagtttgtgc
     2281 cggtgttgat gcattggtaa ttcccagcag aggtgctgcg caaataaatg ccgatgccgt
     2341 aaaaacatca gccggggcac tgcataaatt agctgtttgc aaggtaaacg acctgaaaca
     2401 ggaaatacaa tttcttaaaa attcgggttt gcaaatagtg gcatgtactg aaaaaacaaa
     2461 tcaattgcat tttgatgtgg cctacaccca accaacagca ataattatgg gtgcagaaga
     2521 taccggtata tcgcccgagt ttttaaaact tagcgatgtt aaagttcgca taccaatgtc
     2581 gggtttaatt gactcgttaa atgtttcggt atcggcagca attgttatgt atgaagccgt
     2641 tcgtcagcgc aatacgctta aataaattat tgcgtatatg gtttaagtta ttcataacgc
     2701 aaagcctcaa tgggatctaa tttggcggct tttgctgccg gataatatcc gcttactata
     2761 ccaactgtaa aacaaagtgc tacggcaagc agtatccagt ttagaggcaa aagaaatggg
     2821 ctaccgataa ataaacttaa tgtattgcca ataccaattc ccaaaaacac acctaacaat
     2881 ccgcctaact gacaaatcat tattgattcg gctaaaaact gatgcctgat attggcagcc
     2941 gtagcgccca tactttttct gattcctatt tcgcgtgtac gttcggttac agatacaagc
     3001 ataatattca tcaacccgat tgatgcgcct aataaagtga tggctgcaat agcaattgcg
     3061 gcatatctga tatcacttgt tgactctatt acctgattgg ccaacccatc actttttgtt
     3121 atttcaaaac tattatcact tagtatatca tcacgcctga tgctccgcat taaacctgtg
     3181 gcttcgccta ttgcttgatc taaaaaccga atatcactta catttaccgt ggtggtatag
     3241 gttgtactgt tgctggcata tctgtttttt aaattaagca atggtattat gcaattttta
     3301 tcggggctca taccaaatga cgatcctttt tcttttaaaa cgcctatcac tacatattta
     3361 taactgccta caaatatttg ttgattaata gcaggtttcg atttgaaaag tgtatttgct
     3421 acttcactac ctaaaataat aacacttgct cctaaagtta aatcagtagg cgaaaaatta
     3481 cgacccgttt caagacttaa gccggataca ttcaaatatc catcatcagc acccataata
     3541 aagatattgg ggtttgtttt tttatcgcca aactttaaag tgctgatttg tgatgccatt
     3601 gccgatatgg ataccgtggc aggatatgta aattgttgtt taaacttttg tgcctggtag
     3661 taattaatag atgtatgac
//
LOCUS       DAOMAA010000060         3652 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_18239_length_3652_cov_2.902697, whole genome shotgun sequence.
ACCESSION   DAOMAA010000060 DAOMAA010000000
VERSION     DAOMAA010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18239_length_3652_cov_2.902697"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1225)
                     /locus_tag="PLO59_01380"
     CDS             complement(<1..1225)
                     /locus_tag="PLO59_01380"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HQU99773.1"
                     /translation="MYAINQITTIINGTLFQFSNIETYITSLIIDTRRIENSSNSLFF
                     ALVTSSNNGHKFIADALQKGIRNFVVSDKTIINHTATENHPVAANYILVDDTLLALQM
                     LAASHRHQFQYPVIGITGSNGKTIVKEWLYHLLKEHYNIVRSPKSYNSQIGVPLSVWQ
                     MKNSHTLALFEAGISQPYEMEKLEHIISPTIGILTNISSAHDENFSSRNVKTVEKLLL
                     FKHSRALIYNSDADMVMQHILPLINKTNKQTLTGFSWSKKQKADLEITAISKQADSTT
                     IQASYQNKNFSIVIPFTDEASIDNAITCWATLLYMQVPHENIKAGMRLLPAVAMRLEL
                     KAGINNCSLINDSYNSDMASLGIALDFLMQQQQHNQRTLILSDILQSGQSENDLYEAV
                     STMVMQKKLHCFIGIG"
     gene            complement(1253..1843)
                     /locus_tag="PLO59_01385"
     CDS             complement(1253..1843)
                     /locus_tag="PLO59_01385"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HQU99774.1"
                     /translation="MFLEHRHQQWPKRGWIEVICGSMFSGKTEELIRRLKRAQIAKLK
                     VEIFKPKIDNRYSEAQIVSHNANAIVSTAVHTSSEILSHIGTCEIVGIDEAQFFDDAL
                     PAVCTQLANSGVRVVVAGLDMDFTGKPFGSMPQLLSIAEYITKVHAICMHCGELAHNS
                     HRLAADNAQILVGEKESYVPLCRSCFNTVYMPKNNG"
     gene            2011..3168
                     /locus_tag="PLO59_01390"
     CDS             2011..3168
                     /locus_tag="PLO59_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99775.1"
                     /translation="MSKEIIKDELVHLLDTIVEQTQTVCNYQNKVPQIEIDILMMNVR
                     SFYEQLVYMQKQSNQALPAAKVNPVPVSLSASAQLKPNDSLNEQLHHIEAAIQPQVQV
                     AENDFKPKSIETATTNTQTTGDKVVVQTPVAPVINKPEVLANNAALPVVITPETKPAQ
                     PAIAAQAVKPNVQSDFQPSVGERTVLQKPELTDVIVAVKPETKPESLSPKIENEKVSI
                     AFEIPAVQNVAPIVASTIQFDERKNVAQPIQPQKNVESAELKTKKAVKTTASLFDAPP
                     NIASVFDDTTTLATKLETQQTTTSIADKLNSRKIADLKKAIGINEKFLFINELFEGSL
                     ASYNEHIEKINVAADVHHAHAIIDALISKYAWDGSLSIVKQFKDLVDRRFL"
     gene            3172..>3652
                     /locus_tag="PLO59_01395"
     CDS             3172..>3652
                     /locus_tag="PLO59_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99776.1"
                     /translation="MNRLVLQLFRITAFLLLVNVINAGAQVSKKAHKRIAKLTSEKFF
                     GRGYVKGGDSIAADYLAKQFKQTGLLPIDANYFQKFTFSVNTFPNQIFVNIAGKALKP
                     GYDFIVEAASGRADVTCKLFNVTDSVLPLPQSECAYVISKKYFTTASNKTALKNWLLT
                     "
BASE COUNT         1164 a          699 c          682 g         1107 t
ORIGIN      
        1 ctcctattcc aataaagcaa tgcaactttt tttgcataac catggtagat acggcctcat
       61 ataaatcgtt ttcgctttgt ccgctttgca gtatatctga taagatcaaa gtacgttggt
      121 tatgttgctg ctgttgcatt aaaaaatcga gtgcaatacc gagcgaagcc atatcgctgt
      181 tgtaactatc gttaatgagc gaacagttgt taatgccggc ctttaactct aatcgcatgg
      241 ctacagcagg tagcagtcgc atgcctgctt ttatattttc atgcggcacc tgcatataca
      301 acaatgtagc ccaacaggta atagcattgt caatagaagc ctcatcggta aatggtatta
      361 cgatgctaaa gtttttattt tgatagctgg cttggatggt tgtcgagtca gcttgcttac
      421 tgatggcagt aatttctaaa tcagcttttt gctttttact ccaactaaaa cctgtaagtg
      481 tttgcttgtt agttttattg ataagcggca gtatatgctg cataaccata tcagcatctg
      541 aattataaat aagagccctg ctgtgtttaa ataataaaag tttttcaact gtttttacat
      601 tacgactgct aaagttttca tcgtgcgcac tgctaatatt ggtaagtatg cctatagtag
      661 gactaattat atgttcaagc ttttccattt cgtacggttg cgaaatgcct gcttcaaaca
      721 aagctaatgt gtggctgttt ttcatttgcc acacggatag gggtacacca atttgtgagt
      781 tgtaactttt ggggctacgt acaatattgt aatgctcttt aagcaaatga tacagccact
      841 cttttacaat ggttttgccg ttgctgcctg taataccaat taccggatat tgaaactgat
      901 gcctgtgaga ggctgctaac atttgcagcg caagcaatgt atcgtcaacc aaaatataat
      961 tggctgccac cggatgattt tcagttgccg tgtggttgat tatggtttta tcagacacta
     1021 caaaattgcg aatgcctttt tgtaatgcat cggcaataaa tttatgccca ttattactgg
     1081 atgttaccaa agcaaaaaac aaactgttgc tactgttttc aattcgtctg gtatcaatta
     1141 ttaacgaagt tatataggtt tcaatgttcg aaaactgaaa caaagtgccg ttaataatag
     1201 ttgttatttg atttatggcg tacatcgctg ttattggttt taagcacttt ttttaaccat
     1261 tgtttttagg catgtacaca gtattaaaac aactgcggca taaaggaaca tagctttctt
     1321 tttcgcccac taaaatttgt gcattatctg cggcaagcct gtgcgagtta tgtgccagtt
     1381 cgccacaatg catacatatt gcatgcacct tggttatata ctcggcaatg gataatagtt
     1441 gtggcatgga gccaaaaggc ttgccggtaa agtccatatc caaaccggcc accactacac
     1501 gtacaccgct gttagctaat tgtgtacaaa cagcaggcaa ggcatcgtca aaaaactgtg
     1561 cttcgtcaat gcctactatt tcgcacgtac ctatgtggct aagtatttct gacgatgtgt
     1621 gtacagccgt gcttacaatg gcatttgcat tatgcgatac aatttgcgcc tcgctgtagc
     1681 ggttgtcaat tttaggttta aatatttcga ccttaagttt ggcaatttgc gcacgtttta
     1741 gtctgcgtat taattcttcg gttttacccg aaaacatact gccacatatt acttctatcc
     1801 agccacgttt aggccactgc tgatgcctat gttccaaaaa cataatttac taacgattat
     1861 taaaagtgtt aaataatgaa atagtgtata accataccaa atgcagtagg gtagcgtttt
     1921 ttagttgttc aaacatttct gtcaaaatta ggcattaacc tttcattact aattaaatgc
     1981 ttcattattt tggcaaataa ataagatagt atgtcaaaag aaataattaa agatgaattg
     2041 gtgcatctgc tcgataccat agttgaacaa acccaaaccg tttgcaacta tcaaaacaaa
     2101 gtgccacaaa tagaaattga tattttaatg atgaatgtgc gcagttttta tgagcagctg
     2161 gtatatatgc aaaagcagtc gaaccaggct ttgccggcag caaaagttaa tcccgtgccg
     2221 gttagcttat cagcttcggc acaacttaag cccaacgatt ctttaaatga gcagttacac
     2281 cacattgaag cagccataca gccacaagtg caagtagcag aaaacgattt caaaccgaag
     2341 tctatcgaaa cggcaacaac taacacacaa acaacaggcg ataaagttgt ggtgcaaaca
     2401 cctgttgcac cggtaattaa taaacccgaa gtgcttgcaa ataatgcggc attgccagta
     2461 gttattacac ctgaaacaaa acccgcacaa ccagccatcg cggcccaagc tgttaaacca
     2521 aatgtgcaat cggatttcca gccttcggtt ggtgaaagaa ctgttttaca aaaacctgaa
     2581 ttaacagatg tgatagttgc tgtaaaaccc gaaaccaaac ccgaatcatt atcacctaaa
     2641 attgaaaacg aaaaagttag tatagcattc gaaataccgg cagttcaaaa cgttgcacca
     2701 attgtagcaa gtactataca atttgacgag agaaaaaatg tagcacaacc catacaacct
     2761 caaaaaaatg ttgaatctgc cgaactgaaa actaaaaaag cagtaaaaac aacggccagt
     2821 ttgtttgatg caccacctaa tattgcatcg gtatttgatg atacaacaac gcttgcaact
     2881 aaattagaaa cgcaacaaac cactaccagt attgccgata aattaaacag cagaaaaatt
     2941 gccgacctta aaaaagcgat aggtatcaac gaaaagtttt tatttataaa cgaattattc
     3001 gaaggcagtt tggcatcata caacgaacat attgaaaaaa ttaatgtggc agctgatgtg
     3061 catcatgcac atgctattat tgatgcattg ataagtaaat atgcctggga tggctcgctt
     3121 tcaattgtaa aacaatttaa agatttggtt gaccgcagat ttttataaaa gatgaacagg
     3181 cttgtgttgc aacttttccg tataactgca tttttacttt tagtaaatgt aattaatgcc
     3241 ggtgctcagg taagtaaaaa agcgcataag cgaattgcta aacttacatc cgaaaaattt
     3301 tttggccgtg gttatgtaaa aggtggcgat agtattgctg ccgattattt ggctaaacag
     3361 tttaaacaaa caggcttact gccaattgat gccaattatt ttcaaaaatt tacatttagt
     3421 gtaaatacat ttccaaacca gatatttgta aatattgcag gcaaagcatt aaaacccgga
     3481 tacgatttta tagtagaagc agcatcaggg cgtgctgatg ttacttgcaa attatttaac
     3541 gttaccgata gtgttttgcc tttgccacaa tcggaatgcg cttacgtaat cagtaaaaaa
     3601 tattttacaa cggcatctaa taaaactgct ttaaaaaatt ggttgcttac gt
//
LOCUS       DAOMAA010000061         3652 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_18242_length_3652_cov_2.653600, whole genome shotgun sequence.
ACCESSION   DAOMAA010000061 DAOMAA010000000
VERSION     DAOMAA010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18242_length_3652_cov_2.653600"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..569)
                     /locus_tag="PLO59_01400"
     CDS             complement(147..569)
                     /locus_tag="PLO59_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99777.1"
                     /translation="MTLINNRFALLVCAILSSFFLIASCDEDDKCNAGTGGNVVLVTK
                     PQHHGKAIISQAAYLDSAFIKFNTKEFPGANASLYDLVVAGEIGEDHVHISGLKCGDY
                     YIYMSGWDTTINQRVTGGLPYSFSQESGEINLVVPVTE"
     gene            complement(682..1425)
                     /locus_tag="PLO59_01405"
     CDS             complement(682..1425)
                     /locus_tag="PLO59_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99778.1"
                     /translation="MSAFAMLVSCTLISCDKDDDSNSNNTEPGTVNIEFKNRAAGNNL
                     ELNTTTYSNAMGQDFKFTKFRYILSNFRLKKSDGTYYNVPESYYLIDEAVSNSEKVNL
                     TNVPAGTYTALEFIIGVDSARNTSGAQTGALDPTTGMFWTWNSGYIFVMMEGLAASSP
                     SGDLTFHIGGYKGANNCIRTSTVNFSSNLNVGKDKSPELEIDVNVLEMFKAPNAIDFT
                     TLSNTMGGPTSVLVADNYIDMFTFNSITD"
     gene            complement(1547..2560)
                     /locus_tag="PLO59_01410"
     CDS             complement(1547..2560)
                     /locus_tag="PLO59_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HQU99779.1"
                     /translation="MSSLYVLCLTFITLCFAACKDNENESIDVTFKVPAGWPAPVYNN
                     PANPITEAGFKLGRKLFYDPQLSRDNTVSCGSCHQQFAAFANLDHKVSHGIDNLLGTR
                     NAPPMFNLAWHPTFMWDGGVNHIEVMPLAPIGNPVEMDEQIDNVIAKLRADNEYRFMF
                     KEAFGTDSINSQKMLLAFAQFMSLMVSANSKYDKVMDGQAIFSSEEASGYSFFKKHCN
                     GCHTEPLMTNFTYRNNGLDNTFADLGRGLITQQPNDMGLFKVPSLRNIALSKPYMHDG
                     RFQTLEACIEHYNSGIKSSATVDSLVASGIAISTQEKTALISFLNTLTDSTFITDKRF
                     IEQ"
     gene            2772..3140
                     /locus_tag="PLO59_01415"
     CDS             2772..3140
                     /locus_tag="PLO59_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HQU99780.1"
                     /translation="MLLSMPVDYLPIAMMFVVAIGFVLGTMVATHYLGPKRKSVKKLD
                     TFECGIEVQGNARIPFSIKYFLVAILFVLFDVEVIFMYPWAVNFKSLGMYGFVEMLIF
                     MSILLIGYFYIRKKGGFKWE"
     gene            3169..>3652
                     /locus_tag="PLO59_01420"
     CDS             3169..>3652
                     /locus_tag="PLO59_01420"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HQU99781.1"
                     /translation="MTNITKAPDGFEGHGFFATKLDEAVGLARKNSLWPLPFATSCCG
                     IEFMATMASHYDLSRFGSERLSFSPRQADLLMVMGTISKKMGPVLRQVYEQMAEPRWV
                     LSVGACASSGGIFDTYSVLQGIDRIIPVDVYVPGCPPRPEQILDGVLQIQELVSKETR
                     R"
BASE COUNT         1155 a          641 c          667 g         1189 t
ORIGIN      
        1 aaataggaag tgggtcgtaa accggaccgg tagggttaca gctataacaa gcaacaataa
       61 cacttatagc tgctaaagat tttaatcgca ttaaatttta gaaaaaatat tgattagtta
      121 ttattaaaag caatagccta atataattat tcagttacag gtacaactaa atttatttca
      181 ccactttcct gactgaaaga atagggcaaa ccacctgtaa cgcgttggtt aatagtagtg
      241 tcccagcccg acatataaat ataatagtcg ccacatttta aacctgaaat atgaacatgg
      301 tcttcgccaa tttcgccagc aaccactaag tcatataatg atgcgtttgc acccggaaac
      361 tcttttgtat taaacttgat aaaggctgag tctaaataag cagcttgact aataatagct
      421 ttgccatgat gttgtggttt tgtaaccagc acaacattgc ctccggttcc ggcattacat
      481 ttatcatctt cgtcacaact ggcaatcaaa aaaaatgatg ataggattgc acatactaat
      541 aaagcgaaac ggttatttat tagtgtcata tttaatttta ttgtttaact atttggccta
      601 tttacattaa agcaatagtt cgaatattgt tttatttaat tattaagttc ggggttgtgt
      661 gttgtttggt tagtaacaca gttaatctgt aatactgtta aacgtaaaca tatcaatata
      721 attatcggca actaaaacgg aggtggggcc tcccattgtg tttgatagtg tagtaaagtc
      781 aatggcgtta ggtgctttaa acatttctaa aacattaaca tcaatttcca gttcagggct
      841 tttgtctttt cctacattta agttgctgct aaaattaacg gtacttgttc taatacagtt
      901 gtttgcacct ttgtaaccac caatatgaaa agttaagtcg cccgatggtg atgatgcagc
      961 taacccttcc atcataacaa aaatgtagcc gctgttccag gtccaaaaca tgcctgtagt
     1021 tggatctaat gcaccggttt gcgcacctga ggtgttacgt gcactgtcaa cgccaataat
     1081 aaattcaagt gctgtataag tgccggctgg cacattggtt aaatttactt tttcagagtt
     1141 actaacggct tcgtcaatca aatagtagct ttcaggtaca ttataatagg tgccgtctga
     1201 cttttttaag cggaaattac ttagtatata cctaaattta gtaaacttaa aatcttgacc
     1261 cattgcattg ctataggtag tagtgttaag ctccagatta ttaccagcgg ctcggttttt
     1321 aaattcaata tttacggtgc ctggctcagt attattgcta ttgctgtcat catctttatc
     1381 acagctaatt aatgtacatg aaacaagcat ggcgaatgct gacaatctta gtaatgtgtt
     1441 attcatcgaa tttaaaatgt ttaaaagtta tctgttaatt tatggaaggg caatattaaa
     1501 caagttgcta atatttaatg ctgaattata tttttttact gttatcttat tgttcaataa
     1561 aacgtttgtc ggtaataaaa gtgctatccg ttaaagtgtt taggaaacta ataagtgctg
     1621 ttttttcttg tgtcgaaatt gcaattccag atgctactaa tgaatcaaca gttgctgatg
     1681 atttaattcc cgaattataa tgttcgatac aagcctccaa tgtttgaaaa cgcccatcgt
     1741 gcatataagg tttgctaagc gctatattgc gtaaagaagg aactttaaat aatcccatat
     1801 cattgggttg ttgcgtaatt aagccacgac ctaaatctgc aaaagtattg tctaaaccat
     1861 tattgcgata agtaaagtta gtcatcaaag gctctgtatg gcaaccatta caatgttttt
     1921 taaaaaaact ataaccgctt gcttcttcag aagaaaatat tgcctgcccg tccattactt
     1981 tatcatactt actgttggca gataccataa gcgacataaa ttgcgcaaag gccaataaca
     2041 ttttttgtga gtttatagag tcagtgccaa aagcttcttt aaacataaaa cggtattcat
     2101 tatctgccct aagttttgca ataacattat caatttgttc atccatttct acaggattac
     2161 caattggcgc caaaggcatt acctcaatat gatttacacc accatcccac ataaaagttg
     2221 ggtgccatgc caggttaaac atgggtggag catttctggt acctaataag ttatcaatac
     2281 cgtggctaac cttatgatct aaatttgcga aagcagcaaa ttgctgatga caactaccgc
     2341 aagaaacggt attatcgcgc gaaagctgtg ggtcataaaa caatttacgc cccaacttaa
     2401 aaccagcctc cgtaatcgga tttgccggat tattatatac aggagcaggc caaccggctg
     2461 gcactttaaa agttacatca atactttcat tttcattgtc tttacaagct gcaaaacaca
     2521 atgttataaa agtgaggcat agcacataaa gtgaagacat attttgcata gccgcaaaag
     2581 tatcatttaa aattagaaat tggtttcgat aaaattagca actgaaaaaa cagaatagtt
     2641 taaaatcaaa acaattagaa tatcactatt aaaacttctt aaaaagtaat acttcgcaat
     2701 taagaattta caagtattat tgcagccaaa agccaattta aaaatcaaat tatttaaaac
     2761 cacagtaaat tatgttgtta agcatgccag ttgattatct gcccatagca atgatgtttg
     2821 tagtagctat tggatttgta ttaggtacta tggttgcaac acattatttg ggtcccaagc
     2881 gtaagtcggt taagaaactc gatactttcg aatgtggtat cgaagttcaa ggcaatgcac
     2941 gtattccatt ttcgattaaa tattttttgg ttgccatctt atttgttttg tttgatgttg
     3001 aggttatatt tatgtaccca tgggcggtta attttaaatc gttaggtatg tatggatttg
     3061 ttgaaatgct tatttttatg agcatacttt taattggata tttttacatc cgcaaaaaag
     3121 gcggctttaa atgggaataa gcattttggt aagttttaaa ataaaataat gacaaatatt
     3181 actaaagcac ctgatggttt tgaaggtcat ggtttttttg caaccaagtt agacgaagca
     3241 gtaggattag cccgtaaaaa ttctttgtgg ccattacctt ttgctacttc atgttgtggt
     3301 attgagttta tggcaaccat ggcttcgcat tacgacttat cgcgatttgg ttctgagcga
     3361 ctaagttttt cgccccgcca agccgatttg ctgatggtta tgggtaccat ctcaaaaaaa
     3421 atgggccccg ttttacgaca agtttacgag caaatggccg aacccagatg ggttctttca
     3481 gttggagcct gtgcaagtag cggtggtatt tttgatacct acagtgtgtt acaaggtatt
     3541 gaccgcatca ttcccgttga tgtttatgta cccggatgcc cgccacggcc cgaacaaatt
     3601 ttagatggtg tactgcaaat acaagaattg gttagtaaag aaaccaggcg ca
//
LOCUS       DAOMAA010000062         3642 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_18336_length_3642_cov_2.504321, whole genome shotgun sequence.
ACCESSION   DAOMAA010000062 DAOMAA010000000
VERSION     DAOMAA010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18336_length_3642_cov_2.504321"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..1042
                     /locus_tag="PLO59_01425"
     CDS             134..1042
                     /locus_tag="PLO59_01425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HQU99782.1"
                     /translation="MKVCIAGGSGLIGTHLTHLLLQNKISVIHLSRNKSKTTVPVFKW
                     DIKNSFIEEGALHDVDAIINLAGAGIVAKPWTTAYMQEIVSSRVNAAHTIAVNLQKQN
                     HKVQTYIGASAIGFYGNNPQLNISENTAVQNPDFVSACCKQWEESHALIQASGLRNIT
                     IRIGIVLSNNGGALSEMLKPFNFGLGVYFGNGQMQTSWIHINDLCRAILFLLQNQSIT
                     GTYNLTGCKPVTNKQLIKAAGKIKKHVALMPLPLFVLQLMLGKRAKVVLQSVHVSSQK
                     LQQAGFEFEFADINEVLNNLLQTYSR"
     gene            complement(1043..2266)
                     /locus_tag="PLO59_01430"
     CDS             complement(1043..2266)
                     /locus_tag="PLO59_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008241782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HQU99783.1"
                     /translation="MSKQKIYNDPVYGFITIPTPLVAALIAHPFFQRLRNIKQLGLTH
                     YVYPGALHTRFHHALGAMHLTMQAIETLRSKGVAINDDEAEALIIAVLLHDMGHGPFS
                     HALENAIVQGVQHEWLSLRFMQHLNLLHNGKLQMAMDIFMDVYPKKFLHQLVSGQLDM
                     DRLDYLNRDSFFTGVTEGVVSSDRIIKMLDVYNDTLVVEQKGIYSVEKFITARRLMYW
                     QVYLHKTVLAAENLLLQILKRARFLAFKGHTLFATPAFTYFLTQPVTQAFFEQDEVLQ
                     QFALLDDTDVFASVKVWCNSTDKVLALLCSSLVNRNLYKTTLQTAPIESGIVQQWQTK
                     AVTVLGIKPDDAAYFAFAGTVANNAYQLSDENIHIMYKDGTLLDIAQASDNLNISVLA
                     QPVVKHFLCLHRNLF"
     gene            2426..>3642
                     /locus_tag="PLO59_01435"
     CDS             2426..>3642
                     /locus_tag="PLO59_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQU99784.1"
                     /translation="MTDKPSILWADDEIDLLKPHLLFLNDKGYDVTTVNNGDDALEKI
                     KQQEFDIVLLDENMPGISGLETLVQLKNIKPHLPVIMITKSEEETIMEDAIGGKIADY
                     LIKPVNPNQILLSLKKNLDNKRLISEKTNSKYQQEFRQISMTLGDKLNWQAWTDVYRK
                     LVYWELELEKTQDSGMYEILTSQKEEANMMFSRFVENNYFNWIKNNETAPTLSHQLFK
                     KKVFPLLDQSDEPVFFLLIDNLRYDQWKIIEPVISEYFRVLEEDTYCSILPTATQYAR
                     NAIFAGLLPVDIEKRFPKLWLNDEEEGGKNLHEKEFLADQLQRHRKPCTYTYTKVTNI
                     NAGRDMVENIHNMFQHKLNVIVYNFVDMLSHARTEMEVIRELADDEKGYRSITLSWIQ
                     NSPLMEAIKKIAER"
BASE COUNT         1269 a          694 c          674 g         1005 t
ORIGIN      
        1 tttaaaaaat ggctggtttg caaaagcagg tattgcattc aggtttggac cacaacccag
       61 atttggtaaa aaccatggtg ccaactttat acttgattat gatataacca atatgcactg
      121 taattaaaac attatgaaag tatgcatagc aggcggaagc ggtttaatag gtacacatct
      181 gacacatttg ctgctgcaaa acaaaataag tgtaatacac ctgagccgca ataaaagcaa
      241 aactacggtg cccgtattta aatgggatat taaaaatagt tttatcgaag aaggtgcatt
      301 acatgatgtt gatgcaatta taaatttagc cggtgcaggt attgtagcca aaccctggac
      361 aacagcttac atgcaggaaa ttgttagcag ccgtgttaat gctgcgcata ccattgcagt
      421 taacttacaa aaacaaaatc ataaggtaca aacttacatt ggtgcttcgg ctattggctt
      481 ttacggcaat aacccacagt taaacataag cgaaaacaca gccgtacaaa accctgattt
      541 tgtatcggcc tgttgcaaac aatgggaaga aagccatgcg cttatacagg catccggttt
      601 gcgaaatatc accatacgca taggcatagt gcttagcaac aacggaggtg cactaagcga
      661 aatgcttaag ccttttaatt ttggtttagg tgtttacttt ggtaatggac aaatgcaaac
      721 atcgtggata catataaacg atttatgccg cgcaatactt tttctattac aaaaccaaag
      781 tataacaggt acctataatc ttactggatg taagcctgta accaataaac aacttataaa
      841 agcggctggt aaaattaaaa agcatgtagc gttaatgcca ttaccgttat ttgtacttca
      901 attgatgttg ggcaaacgtg cgaaagtagt tttacaaagt gtccatgtat catcacaaaa
      961 attacagcaa gccggtttcg aatttgaatt tgcagatatt aatgaggttc taaataattt
     1021 gttgcaaact tatagcaggt aactaaaaca aattacggtg taaacataaa aagtgtttta
     1081 caacaggttg tgccaaaacc gaaatgttta aattatccga agcctgtgct atgtcgagca
     1141 aagtgccatc cttatacatg atatgaatgt tttcatcgct aagttggtat gcattattgg
     1201 caacggtacc agcaaaagca aagtaagctg catcatcagg tttaataccc aaaaccgtaa
     1261 cagcttttgt ttgccattgc tgcacaatgc cactttcaat aggtgcagtt tgtaaagttg
     1321 ttttgtacaa attacgatta actaagctgc tgcaaagcaa agccaaaact ttatcagtgc
     1381 tattgcacca cacttttacc gaagcaaaaa catcagtatc gtccaataat gcaaattgct
     1441 gtaacacttc gtcttgctca aaaaatgcct gtgttacagg ttgcgttaaa aaataagtaa
     1501 aggccggtgt agcaaataat gtgtgtcctt taaaagccaa aaaacgcgca cgttttaaaa
     1561 tttgcaacaa taagttttcg gcagctaata cggttttatg caaatacact tgccagtaca
     1621 ttaacctgcg ggccgttata aatttttcga cagagtaaat gcccttttgt tcaaccacta
     1681 atgtatcatt atacacatcc aacattttta ttattctatc gctgctaacc acaccttcgg
     1741 ttacaccggt aaaaaaacta tcgcggttca aataatcgag cctatccata tccaactgac
     1801 ccgataccaa ctgatgcaaa aatttttttg gatatacatc cataaaaata tccatagcca
     1861 tctgaagttt gccgttatgc aaaaggttta aatgctgcat aaaacgtaac gacaaccact
     1921 catgctgtac accttgtacg atggcatttt cgagggcatg cgaaaacgga ccatgtccca
     1981 tatcatgcaa aagcacagca attatcaggg cttcggcctc atcatcattt atcgccacac
     2041 ctttactgcg taatgtttca atagcctgca tggttaaatg catggcgccc aatgcatgat
     2101 gaaaacgcgt atgcaatgca cccggatata cataatgtgt aagccccaat tgtttgatat
     2161 tgcgcaaacg ctgaaaaaac ggatgggcaa ttaaagccgc taccaatggt gtaggtatgg
     2221 taataaaacc atacacaggg tcgttatata ttttttgttt actcacagcc tgcaaagcta
     2281 ataactagta aatgccaaat acatgtatga ttttatgttg ttgatgttat atttttatca
     2341 aaacaaaata tgtaacggct gcattacgta gtaaaactta gcttagccgg tatcataaat
     2401 taccaactca aaacaacaca taaatatgac tgataaacca agcatacttt gggccgatga
     2461 tgaaattgat ttattaaagc cccatttact ttttttaaat gataaaggtt atgatgtaac
     2521 caccgtaaat aatggtgacg atgcccttga aaaaattaaa caacaagaat ttgatattgt
     2581 gttgttagac gaaaacatgc ctggtatatc gggcctcgaa accttggtgc aacttaaaaa
     2641 cattaagcca cacctgccgg taattatgat taccaaaagc gaggaagaaa ccattatgga
     2701 agatgccatt ggcggtaaaa ttgccgatta tctgattaag cccgtaaacc ccaatcagat
     2761 attgctatcg cttaaaaaga atttagataa caaacgtttg ataagcgaaa aaaccaattc
     2821 gaaatatcag caagagttca ggcagataag tatgacctta ggagataaac ttaactggca
     2881 agcctggacc gatgtatata gaaaattggt gtactgggag ttggaattag aaaaaacaca
     2941 agacagcggc atgtatgaaa tacttaccag tcaaaaagaa gaagccaata tgatgtttag
     3001 ccgttttgta gaaaacaact attttaactg gataaaaaat aacgaaacag cccctaccct
     3061 atcgcatcag ctttttaaga aaaaagtttt tccgttgttg gatcaaagcg atgaacccgt
     3121 atttttttta ctgatagata atttacgtta tgatcaatgg aaaataattg aacccgttat
     3181 tagcgaatac tttcgggtgt tagaagaaga tacctattgc agcattttac ccacagccac
     3241 acaatatgcg cgcaatgcca tatttgccgg cttattgcct gttgatattg aaaaacgatt
     3301 tccgaaactg tggctgaacg atgaagaaga aggtggaaaa aatttgcatg aaaaagaatt
     3361 tttagccgac cagttgcaac gccaccgcaa gccctgtacc tatacttaca ccaaggtaac
     3421 caatataaat gcaggccgcg atatggtaga aaacatacac aatatgtttc aacataagct
     3481 taatgtaatt gtatataatt ttgtagatat gctaagccat gcacgcaccg aaatggaagt
     3541 aatacgcgag cttgcagacg atgaaaaagg ctaccgaagc attaccctat cgtggataca
     3601 aaactcacca ttaatggagg ctattaaaaa aatagccgaa cg
//
LOCUS       DAOMAA010000063         3633 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_18428_length_3633_cov_3.098658, whole genome shotgun sequence.
ACCESSION   DAOMAA010000063 DAOMAA010000000
VERSION     DAOMAA010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3633
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18428_length_3633_cov_3.098658"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="PLO59_01440"
     CDS             <1..476
                     /locus_tag="PLO59_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99785.1"
                     /translation="HFNGIKTNPYSKTYTDYVSRQIDGKNMVAASIINGVSYDRIFAK
                     NTQFRVNDAYLYYINPTYKCHAISVMDGYNAKGNKALEKLFVEQSVFYQFVQHQKATG
                     VFQDIAQSQIDFIKEKKIKLVFVNKGSYVADSIIALADTTLTDTGTGETLLMLSY"
     gene            complement(479..1546)
                     /locus_tag="PLO59_01445"
     CDS             complement(479..1546)
                     /locus_tag="PLO59_01445"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HQU99786.1"
                     /translation="MAQPDFFKALTGIRAIAAYMVFLHHYNPFKDIAGCEILWGIVNE
                     FHTGVTLFFVLSGFLIAHKYLFDSNTAFKKYFFRRFARIYPLFFILTTITFLWIGIQS
                     PSSKMLLNLYFYNITFLKGLFADIKFTGIAQGWSLTVEEIFYLTAPLWFLAIRKNKIA
                     MVVLPVIIFILGCLIVKTSQSIGCKSFMSSYGFMAIYTFMGRCFEFFAGIALAYMLHL
                     KIKFRFKINYSLLGVLNFLVCIYLISIFKGNHVAGIHHPAGVLINNLILPVFAIVPLF
                     YGLIFEKTLLQKFLSVPFVQLLGKSSYAFYLLHMGVFMSAILKMGLPVWLCFVLITVL
                     SIICFKLIEEPLNIFIRKRFA"
     gene            complement(1593..>3633)
                     /locus_tag="PLO59_01450"
     CDS             complement(1593..>3633)
                     /locus_tag="PLO59_01450"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HQU99787.1"
                     /translation="KDSAIFGLPFYIEWIKQDQFNLSYTFNGNKVTADYNINKWIELP
                     ELRLKITLNSYNTILDQQKTFKNDAYYFTINNLDVEAAKYASALLVGIVNSDAKTLYV
                     RLKDNSPTKAADIVNAITTEFIGYDLKKNTEVTNKILNFIDTTVSNIQHALILSEDSI
                     EEFKRRNKFFDPDDYGNMLNSEMRRFDDLRFDLEYKLALIDKLKSEMKAKRDITTMLI
                     SLSGVYKEGNINEATTELLKLLKDKEQLLNEVTTSAPNYSFIEAAIANQVQILNAHIK
                     NEESSLRLRMAQIDKQLGQYNSTMVVLPSRQTEYARLIRINKINEKFYSLLLEKKAEF
                     AITKAGYDSDNIILEPAIPRFVPISPNKPLILSGCITLGFFLSLGLIIFRYLMYNEIT
                     SLEEVSLYTDAALLGMIPKYKREIPVSQLLVDKNPKSIISEAFRSVRTNLQFIINDET
                     AKVFAVTSTISGEGKTFNAINLAGVIAFSGKRVAILDLDMRKPKIHLGFNVENLHGMS
                     TILIGKDSVKNCIQKSSLPGLDFITAGPIPPNPAELIISQRMEDLIQELKLSYDVVII
                     DTPPIGIVSDGIPLIQKVDLPIYILRSRYSKKMFIHNINKLLDESKVKHLSVILNGVD
                     TSKNGYGYGYGYGSSYGYGYGYGYGYGYGYGYGYGYYDEDDKPNIFQRILKTIGL"
BASE COUNT         1213 a          622 c          608 g         1190 t
ORIGIN      
        1 ttcattttaa tggcataaaa acaaatccat acagcaaaac ctatacagat tacgtgagcc
       61 gtcaaattga tggtaaaaac atggttgctg catcaataat aaatggagta agttacgacc
      121 gcatttttgc aaaaaacaca cagtttaggg ttaacgatgc atacctttat tatatcaatc
      181 caacatataa atgtcatgct atatcggtta tggatggtta taatgctaaa ggaaataaag
      241 cgttggagaa attgtttgta gagcaatctg tattttatca gtttgtgcaa catcaaaaag
      301 caacgggtgt ttttcaggat atagcacaaa gtcagattga ttttataaaa gagaaaaaaa
      361 taaaacttgt atttgtaaac aaaggcagct atgtagccga ttcaattatt gccttagctg
      421 atactacttt aaccgataca gggacaggcg aaactttatt aatgttatcc tattagtttt
      481 atgcaaatcg tttacgaata aaaatattta gcggctcctc aatcagttta aagcatataa
      541 tagaaagtac agttatcaaa acaaaacaga gccaaacggg taaacccatt tttaaaatag
      601 cactcataaa aacgcccatg tgcagtaaat aaaaagcata cgagcttttg ccgagtaatt
      661 gcacaaaagg taccgacaaa aatttttgta aaagtgtttt ttcgaatatt agtccgtaaa
      721 aaagcggcac aatagcaaac accggtaaaa tcaaattatt aatcagaaca cctgcaggat
      781 gatgtattcc ggcaacatga ttgcccttaa aaatcgatat taaataaata caaactaaaa
      841 agttaagtac accaagcaaa ctgtagttaa ttttgaaacg aaattttatt tttaagtgaa
      901 gcatataggc taatgctatg ccggcaaaaa attcgaaaca acgacccata aaagtatata
      961 tggccataaa accatagcta ctcataaaac ttttacatcc gatgctttgc gaagttttaa
     1021 cgatgaggca tcccagtata aaaattataa caggcaatac gaccattgca attttatttt
     1081 ttcgaattgc taaaaaccaa agcggtgcgg ttaagtaaaa aatttcttca accgttagtg
     1141 accatccttg cgcaatgcct gtaaacttta tatcggcaaa caaacccttc agaaaggtaa
     1201 tgttatagaa gtataagttt aaaagcattt ttgatgaagg gctttgtatt ccaatccata
     1261 aaaaggtaat tgtagtaaga ataaaaaata acggatatat acgtgcgaac cgtctaaaga
     1321 agtatttctt aaaagctgta tttgaatcga acaaatattt atgtgcaatt aaaaatccac
     1381 ttagcacaaa aaacaacgta acacctgtat gaaattcgtt tacaatgccc cacaatattt
     1441 cgcaacctgc aatatcttta aatgggttgt aatgatgcaa aaacaccatg tatgctgcta
     1501 tggctcttat accggttaaa gccttaaaga aatccggttg tgccattatt aaaatttaaa
     1561 caggttggag ttacaaaagc acctattagg ttttataagc cgattgtttt taaaatccgt
     1621 tgaaagatgt tgggtttatc atcttcatcg taataaccat aaccatagcc atatccgtag
     1681 ccgtagccat atccgtaacc atagccataa ccataactgc ttccatatcc gtaaccgtat
     1741 ccatagccat ttttgctggt atcaacaccg ttaagtataa ctgataagtg ttttacttta
     1801 ctttcgtcaa gtagtttatt tatattatgt ataaacattt ttttactgta acggctacgc
     1861 aaaatataaa taggcaaatc aactttttga attaaaggaa taccatcgct aacgatacca
     1921 ataggtggtg tgtcaattat tacaacatca tacgaaagtt ttaattcctg aattaaatct
     1981 tccatacgct gtgatataat taattcggca gggttagggg ggatagggcc tgctgtgata
     2041 aaatccaaac cgggtaagga gcttttttga atacagtttt taaccgagtc tttgccaatt
     2101 aatatggtac tcatgccatg caaattttcg acattaaacc ctaagtggat tttgggtttg
     2161 cgcatgtcta aatctaatat agccacacgt ttaccgctaa atgcaattac gccagccagg
     2221 ttaattgcat taaatgtttt tccttcgcct gaaatggtac ttgtaacagc aaatactttt
     2281 gctgtttcat catttattat aaactgtaaa ttagttcgta cgcttctgaa agcttcagat
     2341 attatagatt tcgggttttt gtcaaccagc aattgcgata caggtatctc gcgtttatat
     2401 ttaggtatca tacccaacaa ggcagcatcg gtatatagac taacttcttc taacgaagtt
     2461 atttcgttat acatcagata tctgaaaatt attaaaccta agcttaaaaa aaagcccaat
     2521 gtaatgcagc cacttagtat aaggggcttg ttaggtgata taggcacaaa gcgtggtatg
     2581 gccggctcaa gtataatatt gtcagagtcg tagcctgctt tagtaattgc aaactcagct
     2641 ttcttttcta ataagagtga gtaaaatttt tcgtttattt tattaattcg gataaggcgt
     2701 gcatactctg tttgacgcga tggcaaaaca accatggtgc tattatattg cccaagttgt
     2761 ttatcaattt gagccatgcg aaggcgcaaa cttgattctt cattttttat atgtgcattt
     2821 aaaatttgca cctgattggc aatagcagct tctataaaac tataattagg tgcgctggta
     2881 gttacttcgt tcagtagttg ttctttgtct tttaatagtt ttagaagctc tgtagttgct
     2941 tcattaatat taccttcttt ataaacgcca ctaagactta taagcatggt tgttatatca
     3001 cgcttggctt tcatttctga ttttaattta tcaatcaatg ccagtttgta ttccaggtca
     3061 aagcgtaagt cgtcaaacct gcgcatttcg ctgttaagca tattaccata atcatccgga
     3121 tcgaaaaatt tatttcttcg tttaaattct tcaatcgaat cttctgataa aatcaaagca
     3181 tgttgaatat tgctaacggt tgtatcaata aaatttaata tcttattggt aacttctgta
     3241 ttctttttta aatcgtagcc tataaactca gtggttatag catttacaat gtctgcagct
     3301 tttgtaggac tgttgtcttt taaccgtacg tataatgttt ttgcatcaga gtttacaata
     3361 ccaaccaata gtgctgatgc gtatttggct gcttcaacat ctaaattatt tatcgtaaag
     3421 tagtaggcat cgtttttaaa tgttttctgc tgatcgagaa tggtattgta actgtttaaa
     3481 gttattttta aacgcaattc gggcaattca atccacttgt ttatgttgta atcggctgtt
     3541 actttattgc cgttgaatgt ataacttaaa ttaaactggt cttgcttaat ccattcaatg
     3601 taaaatggca acccaaatat tgctgagtct tta
//
LOCUS       DAOMAA010000064         3613 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_18606_length_3613_cov_3.034851, whole genome shotgun sequence.
ACCESSION   DAOMAA010000064 DAOMAA010000000
VERSION     DAOMAA010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18606_length_3613_cov_3.034851"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            13..1374
                     /locus_tag="PLO59_01455"
     CDS             13..1374
                     /locus_tag="PLO59_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HQU99788.1"
                     /translation="MVKKTVRKAQAKNNKKIFVLDTSAILFDHNVIQNFKEHDVAIPI
                     TVLEEVDRFKKGNDVINFEAREFIRSLDTLSQSFTLNDWIPLNGPTKGKLKVLMNEDS
                     TVDANKVFNEKKADHNIINAALRLQEDLPGRKVVLISKDINLRLKAKSLELTSEDYNT
                     GKVKDLEGLYTGCSVVENLQRKLIDALYTQGSYHYKSIFKTRPLANHYFILKSKNDEV
                     SALAYFNAETDCIERIQKENASGIKPRNAEQAFAMHAVLNPSVKLVTVQGIAGTGKTL
                     IALASALEQKSSYRQIYLARPIVPLSNKDIGYLPGDIKSKINPYMEPLWDNLKFIQSQ
                     YAETDKEFKKINEMVANEKLHVTPLAYIRGRSLSNICFIIDEAQNLTPHEVKTIITRA
                     GENTKIIFTGDVFQIDTPYLDSHSNGLSTLIDKVKGQSIYAHITLEHGERSELSNLAG
                     TLL"
     gene            complement(1380..1709)
                     /locus_tag="PLO59_01460"
     CDS             complement(1380..1709)
                     /locus_tag="PLO59_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009087188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HQU99789.1"
                     /translation="MQQFALTLDLKDDKNLIAEYEAYHQQVWPEIIDSIKNTGIISLK
                     IYRWHCRLFMLLIADEDFSFETKNKADANNAMVQKWETLMWQYQQALPATQPGSKWQL
                     MDEIFSL"
     gene            complement(1718..2908)
                     /gene="fucP"
                     /locus_tag="PLO59_01465"
     CDS             complement(1718..2908)
                     /gene="fucP"
                     /locus_tag="PLO59_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015535 - fucose:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HQU99790.1"
                     /translation="MIQKNTQLLPLVLIVSLFFLWGLAHNLNPILIPHLKSACNLSDF
                     QSALIDSAFFIAYFIFAIPAGIYMSYYGYKNGIVLGLLLFAAGAFLFIPAANTLSYPI
                     FLMALFVIAAGLTFLETAANPYVTILGNSSSAVMRLNLAQSFNGLGAFIAPLIGGMFI
                     LSDTHQSDAVQSTVIKVPYIIIGLVVFIVALTFIKIKLPEIADNTKPEPWQTALRHKK
                     LLAGITAQFFYVGAQVGVSSFFIRYLNSVLDISQQQAAYYLSFALVLFMTGRFTGTVL
                     MKYITPIKLLLVFALVNIVLLLPVIAGGNLALYAIIGVPFFMSIMFPTIFSLSIQDTG
                     NFTKQASSLIIMSIVGGAIIPLIMGAISDTWSIRWGYVAPLFCFLFVAWFAYYFSRIK
                     PHNN"
     gene            complement(2916..>3613)
                     /locus_tag="PLO59_01470"
     CDS             complement(2916..>3613)
                     /locus_tag="PLO59_01470"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytidyltransferase"
                     /protein_id="HQU99791.1"
                     /translation="HDQPYVSKHAPGSVITIAIEPTIDFNHRSGMSSSTRHKAIELWQ
                     NDIPAGNKEQLAKILFAYENPPGTQTIAGSQDSIGIVYPGLNRLDYNGGFWPTHIESI
                     LDESLLNWIEAHLYLITLAPRTADYNVLSKTNIASYHVKALATATQHCWQGLINKDIK
                     MAGQGMLQSFHAQLEMFPNMVDAHIMQLINEVKHQAIGYKLSGAGGGGYLIMFADKPV
                     PNSIKIKIRRAGF"
BASE COUNT         1248 a          640 c          653 g         1072 t
ORIGIN      
        1 atttaaggta ctatggtaaa aaaaacagtg cgtaaagctc aggcaaaaaa caataagaaa
       61 atttttgtgc tcgatacatc agccatttta ttcgatcaca acgtaataca aaattttaag
      121 gagcacgatg tagccatacc cataactgtt ttagaagaag ttgatagatt taaaaaaggt
      181 aatgatgtta ttaactttga ggcaagagag tttatcagat cattagatac acttagtcaa
      241 agttttacat tgaatgattg gattccatta aacggaccta caaaaggtaa acttaaggtg
      301 ttgatgaatg aagatagcac agttgatgcc aataaagttt ttaatgaaaa gaaagctgat
      361 cataacataa ttaatgctgc cttaagattg caggaagact tgccgggccg caaagtggta
      421 ttaattagta aggatataaa tcttcggcta aaagcaaaat cgttggaatt aacatctgaa
      481 gattataata ccggcaaagt aaaagattta gaaggccttt acaccggttg cagtgtagtt
      541 gaaaatttgc agcgtaaatt aattgatgct ttatataccc aaggtagtta tcattataaa
      601 tctattttta aaacaaggcc attagccaac cattatttta ttttaaaaag taaaaatgat
      661 gaagtaagtg ctttggcata ttttaatgcc gaaaccgatt gtattgaaag aatacagaaa
      721 gaaaatgcaa gtggcataaa gccccgcaat gccgagcaag cttttgccat gcatgccgtt
      781 ttaaacccca gtgtaaagtt agtaacggtt caaggcattg ccggtaccgg taaaacctta
      841 attgcattgg catctgcctt agaacaaaaa agcagctata ggcaaatata tttagcccgg
      901 cccattgtac ctttaagcaa caaggatatt ggctacttgc ccggagatat taaatcgaaa
      961 attaatcctt acatggagcc actttgggat aatttaaaat ttattcaaag tcaatatgcc
     1021 gaaacagata aagagtttaa aaaaataaat gaaatggtgg ccaatgaaaa actacatgta
     1081 acgccattgg cttacatacg tggccgcagt ttaagcaaca tttgttttat aattgacgaa
     1141 gcacaaaatt taacgccaca tgaagtaaaa accattatta caagagcagg cgaaaacaca
     1201 aaaattattt ttaccggtga tgtttttcaa attgatacac cttacctcga ctcgcacagc
     1261 aatggattgt caacacttat tgataaagta aaggggcaat caatttatgc acacattact
     1321 ttagaacatg gcgaacgaag cgaacttagc aacctggccg gtactttatt gtaaacaaat
     1381 taaagactaa atatttcgtc catcaattgc catttactgc cgggttgtgt tgccggtaaa
     1441 gcttgctgat actgccacat aagtgtttcc catttttgca ccattgcgtt gttggcatcg
     1501 gctttattct tggtttcgaa actaaaatcc tcatcggcaa taagtagcat aaacaaccgg
     1561 caatgccagc ggtaaatttt taaactaata attccggtgt ttttaatact atcaattatc
     1621 tcaggccaaa cttgctgatg atatgcttca tattctgcaa tcagattttt atcgtctttc
     1681 aaatcgagcg taagtgcaaa ttgctgcatg cgtatattta gttattgtgt ggtttaattc
     1741 gagaaaaata gtaagcaaac caagcaacaa ataagaaaca aaaaagcggt gcaacatagc
     1801 cccatcttat gctccaggtg tcggaaatgg cgcccataat taagggaata atagcgcctc
     1861 cgacaatact cataattatt aatgacgaag cctgtttggt aaaattaccc gtatcctgaa
     1921 tgcttaatga gaatatagtt ggaaacataa ttgacataaa aaacggaaca ccaattattg
     1981 cgtataatgc caggttacca cctgcaataa ccggtaatag taaaacgatg tttactaaag
     2041 caaaaacaag taaaagctta atgggcgtta tatacttcat taaaacggtt ccggtaaacc
     2101 ggcctgtcat aaatagaacc aatgcaaacg aaaggtaata tgcagcttgt tgctgactta
     2161 tatctaaaac cgaatttaaa tacctgataa aaaaactact tacaccaacc tgagccccca
     2221 cataaaagaa ttgtgctgtt atgccagcca atagtttctt gtgtcttaaa gcagtttgcc
     2281 agggttcagg cttggtatta tctgcaattt cgggtaattt gatttttata aatgttaatg
     2341 ctactataaa cacaaccaag cctataatta tatacggaac tttaattact gttgattgta
     2401 cagcatcgct ttgatgtgta tcggataata taaacattcc gcctattaat ggtgcaataa
     2461 atgcacccaa gccattaaac gattgtgcta agtttaaacg cataacagca gatgagctat
     2521 tgcctaatat ggtaacataa ggatttgctg cagtttctaa aaaagtaagc cctgctgcaa
     2581 ttacaaataa tgccataaga aatatgggat atgataatgt gttggctgca gggataaata
     2641 aaaatgcacc ggcagcaaat agcaacagtc ctaatacgat tccattttta tagccataat
     2701 agctcatata gatgccggct ggtattgcaa aaataaaata ggcaataaaa aaagcagagt
     2761 caattaaagc actttgaaag tcgctaaggt tacaagcact ctttagatgt ggaattaaaa
     2821 tgggatttaa gttatgtgcc aatccccata aaaagaatag tgatacaatt agaaccaaag
     2881 gcaacagttg tgtgtttttt tgtatcataa tttaatcaaa aacctgctct tctgatttta
     2941 atttttatac tatttggcac tggtttgtcg gcaaacatta taagataacc accaccaccg
     3001 gcaccactta atttataacc aattgcttga tgcttaactt cgttaattaa ttgcattatg
     3061 tgtgcatcaa ccatgtttgg aaacatttct aactgtgcat ggaatgactg caacattcct
     3121 tgtcctgcca ttttaatatc cttatttata agtccttgcc agcaatgttg tgttgctgta
     3181 gctaatgcct taacatgata agaagcaata tttgttttcg acaaaacatt gtaatcagca
     3241 gtgcgtggtg ccaatgtgat taaatacaaa tgtgcttcaa tccagtttaa caaactttca
     3301 tccaaaatag attcaatgtg tgttggccaa aaaccgccat tataatctaa ccggtttaag
     3361 cccggatata caataccaat actatcctgt gagcctgcaa ttgtttgcgt gcccggtgga
     3421 ttttcgtaag caaataaaat tttagccaat tgttctttat ttccggccgg tatatcgttt
     3481 tgccaaagtt cgattgcttt atggcgtgtg ctgctactca taccgctacg atgattaaaa
     3541 tctattgtag gttcgatagc aatagttata actgaaccag gtgcatgttt tgaaacataa
     3601 ggttgatcgt gcc
//
LOCUS       DAOMAA010000065         3611 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_18635_length_3611_cov_2.946850, whole genome shotgun sequence.
ACCESSION   DAOMAA010000065 DAOMAA010000000
VERSION     DAOMAA010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3611
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18635_length_3611_cov_2.946850"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..129)
                     /locus_tag="PLO59_01475"
     CDS             complement(<1..129)
                     /locus_tag="PLO59_01475"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99792.1"
                     /translation="MKVLKFGGTSVAQAENLTKVAAIILKTVKTDPAIVVVSAMSGI"
     gene            complement(211..1470)
                     /locus_tag="PLO59_01480"
     CDS             complement(211..1470)
                     /locus_tag="PLO59_01480"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQU99793.1"
                     /translation="MPYALYLTGTFSNKNYFDLIFQYFIAKRYLIAKKSQQAINIITL
                     ISIIGVMVGTAGLVIVLSVFNGFGNLMVSLYDTFDADIKITATTGKTISVTDSTLTEI
                     QHIEGVAHVFAVYEENVLFKYGDKQVIGKLKGIPLQYADAALQKTIIDGSFAADAYQH
                     FAVFGAGIAYQLGMDLDHVTQPIQMYVPKTGIDNPVNVDDAFNQASALPTGVFSLQQD
                     FDNQYVFVPLTFAQTLFDAPQKATAIEVTLKPNAPTKLVQQALENKNWQVQTRQQQHA
                     FLYAIINSEKFIAYFILVLILIISTFNILGSLTMLIIEKQKDLKLFLSMGATPKKIKQ
                     VFVIQGLMITAVGSMGGLLLGFIVCFCQQHFGIIKIANSQSFVVDAYPVAIVAADFII
                     CMLIVTCIGAIASFYTAHFVVSKYIRS"
     gene            complement(1474..2283)
                     /locus_tag="PLO59_01485"
     CDS             complement(1474..2283)
                     /locus_tag="PLO59_01485"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQU99794.1"
                     /translation="MQYDPIKRSLGNVFNQSVFLRKVFYNLLDLLLLRCWHIHKELRT
                     YKKTAGSNLQIFDAGAGFGQYSYWMYNFFKPCNILAVDVKDEQVADCNQFFNKIGATS
                     NVTFIVGDLTQYQNPNNFNLVLCVDVMEHIEDDNACFKNYHASLKPGGMLLISTPSDQ
                     GGSDVHDGADASFIEEHVRDGYNITDIENKLKAAGFSKIEARYNYGTPGKISWRLSMK
                     YPIQVLGISKLFFVLLPFYYLLVYPFCFVLNYCDTNMQHSTGTGLVVKAWK"
     gene            complement(2317..2709)
                     /gene="rbfA"
                     /locus_tag="PLO59_01490"
     CDS             complement(2317..2709)
                     /gene="rbfA"
                     /locus_tag="PLO59_01490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HQU99795.1"
                     /translation="MESTRQQKFARQIQREIGDIFIKEGKAIIGQTFVTVTQVKITPD
                     LGEARIHLSVLKPANPVTFITHLNKHKGDIRKLLGNRIKNTIHHIPQLLFFIDDSLDY
                     VDNINKVMNNIVIPDHTQVNPADYKSEE"
     gene            2852..>3611
                     /locus_tag="PLO59_01495"
     CDS             2852..>3611
                     /locus_tag="PLO59_01495"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HQU99796.1"
                     /translation="MKDYLKLLVYVRSYKKFAILNVICNILAAIFGVFSITLVIPFIG
                     FLFGKEALVTVAPPLAFNMQSLKENFYFFISKIIADNSLPGNEAAGKIKALIIICCGF
                     LVALFLKNLFRYLAMYYIAFMRNGVIRDIRNAMHKRVLELPMQYYSNERKGDIMSRMS
                     NDVMEIEWSILSGLEFLFREPFTIALTLAILIGLSGQLTLTVFIVLPVAGLIIGILGS
                     SLRRSSGKAQGLMGQLFSNMEETLGGLRIIKAFTA"
BASE COUNT         1093 a          650 c          711 g         1157 t
ORIGIN      
        1 tataccactc atagccgaca caactacaat ggccgggtcg gtttttacgg tttttaaaat
       61 gattgctgca accttagtta agttttcggc ttgcgctact gatgtccccc cgaactttaa
      121 aactttcata aaataaaaat ttgcgtaata ggctggcaac attaccaaaa attacgtcaa
      181 accataaact gaaaatttat ggtgtaagac ttaacttctg atgtatttac ttacaacaaa
      241 atgtgctgta taaaatgagg caatggcacc tatacaagta acaattaaca tacaaattat
      301 aaagtcggca gcaactattg caacggggta tgcatcaacc acaaacgatt gtgagttggc
      361 tatttttata atgccgaaat gttgttggca aaagcaaact ataaaaccta acagtaaccc
      421 gcccatgctg ccaacggcag ttatcatcaa accttgtatt acaaatactt gtttaatttt
      481 tttaggcgtt gcacccatgc ttaaaaacag tttcaaatct ttttgttttt cgataatgag
      541 catagtaagc gagcccagta tattaaaagt agaaataata agaatgagca ctaaaataaa
      601 gtaggctata aatttttcgg aattaattat ggcatataaa aaagcatgtt gttgttgccg
      661 tgtttgcacc tgccagtttt tattttcaag tgcttgctgc acaagtttgg tgggtgcgtt
      721 gggttttagt gtaacctcaa tggcagtagc tttttgtggg gcatcaaaaa gtgtttgcgc
      781 aaaggtaagc ggcacaaaaa cgtattggtt atcgaaatcc tgctgaagcg aaaatacacc
      841 tgtaggcaat gccgatgctt gattaaatgc atcatcaaca tttaccggat tatcaatacc
      901 tgttttgggt acatacattt gaatgggttg cgttacatgg tctaaatcca tgcctaattg
      961 ataagcaata ccggcaccaa aaacagcaaa atgttggtat gcatcggcag caaaactgcc
     1021 gtcaataatg gttttttgaa gtgcagcatc ggcatactgc aatggtatgc ctttaagttt
     1081 gccaattact tgtttatcgc catatttaaa aagtacgttt tcttcgtaaa cggcaaatac
     1141 gtgtgcaacg ccttcgatat gctgaatttc tgttaatgta ctatcagtta ccgaaatggt
     1201 tttgccggta gttgctgtaa tttttatgtc ggcatcaaac gtatcgtata acgataccat
     1261 taagttgcca aaaccattaa aaacacttaa aacaattacc agtccggccg tacctaccat
     1321 tacaccaata attgaaataa gtgtaataat atttatagcc tgttgcgatt ttttggcaat
     1381 caaataacgt ttggctatga agtactggaa aatcaaatcg aagtaatttt tgttgctaaa
     1441 tgtaccggtt aaatacagcg catacggcac gggttatttc catgctttaa ccaccaggcc
     1501 ggtgcccgta ctatgttgca tgttggtatc gcagtaatta agtacaaagc aaaacggata
     1561 aactaagagg tagtaaaacg gcagcagtac aaaaaacagc ttgcttatgc ccagcacctg
     1621 tatgggatat ttcatagata aacgccatga tattttaccc ggtgtgccat agttataacg
     1681 tgcttctatt ttagaaaagc cggcagcttt aagtttattt tcgatgtcgg taatgttgta
     1741 gccatcacgt acatgttctt caataaacga tgcatcagca ccatcatgta catcgctgcc
     1801 accctggtcg ctgggggtcg aaataagtaa catgccaccc ggttttaatg atgcatgata
     1861 gtttttgaaa caggcattat cgtcttcaat atgttccatc acatcaacac ataataccag
     1921 attaaagttg ttggggtttt gatattgggt taagtcgcct acaataaacg ttacgtttga
     1981 agttgcgcct atcttattaa aaaactgatt gcaatcggct acctgctcat cttttacatc
     2041 taccgctaaa atattgcaag gtttaaaaaa gttgtacatc caatagctgt attggccaaa
     2101 gcctgccccg gcatcaaaaa tttgcaaatt actgccggct gtttttttat aagtgcgtaa
     2161 ctctttgtgt atatgccaac agcgtaacag taataagtct aacaggttgt aaaaaacttt
     2221 acgtaaaaaa accgattggt taaaaacgtt acctaacgaa cgtttaatag ggtcgtattg
     2281 cataccgatg tttgtgtgct ttatttagta aaaaaactat tcttcagatt tgtagtcagc
     2341 cggattaacc tgtgtgtggt caggtataac aatgttattc atcaccttat ttatattgtc
     2401 aacataatcc agcgaatcgt caataaaaaa caagagttgt ggtatatggt gtatggtgtt
     2461 tttaattcga ttacctaaca atttgcgtat atcacctttg tgtttattca gatgtgtgat
     2521 aaaggttaca gggttggcag gtttaagtac acttagatga atgcgcgctt cgcctaagtc
     2581 gggtgttatt tttacctgtg taacggtaac aaaggtttgc ccaataatag ccttgccttc
     2641 tttaataaat atatctccta tttcgcgctg tatttgccgc gcaaactttt gttgacgagt
     2701 gctttccatg cagcaaaagt attatttttt atcgggcaat tgtgaatggc actgccatgg
     2761 tgcataggtg taaatgcttt taaccttata tttgcagtta ttataaattg cgcctcaaaa
     2821 tttatttaca ccgtttaaac gcatccactt tttgaaagac tacttaaaat tactggtgta
     2881 tgtgcgcagc tataaaaagt ttgccatact taatgtgatt tgcaacatac tggccgctat
     2941 ttttggtgta ttcagcataa cgttggttat tccatttatc ggctttttgt ttggcaagga
     3001 agcattggta actgtggcgc caccattggc ttttaatatg caaagcctta aagagaattt
     3061 ttattttttc atcagtaaaa ttattgccga caacagtttg cccggtaatg aagctgccgg
     3121 aaaaattaaa gctttaatta ttatttgctg tggcttttta gtggctttgt ttttaaagaa
     3181 cctgtttcgg tatttggcca tgtattacat agcatttatg cgcaatggtg taatacgtga
     3241 tatcagaaac gccatgcaca aacgtgtgct tgagctacct atgcaatatt atagcaatga
     3301 gcgtaaaggc gatattatgt cgcgtatgag taacgatgta atggaaattg aatggagtat
     3361 attaagtggg ttagagtttt tgtttcgtga gccatttacc attgcattaa cgctggcaat
     3421 tttaattggc ttaagcggcc agttaacact tacagtattt attgtattac cggtagcagg
     3481 cttaattatt ggcatattgg gcagcagctt acgcagatcg tcaggtaaag cacagggcct
     3541 aatgggccaa ctgtttagca atatggaaga aacgctgggc ggattaagaa ttattaaagc
     3601 atttactgcc g
//
LOCUS       DAOMAA010000066         3610 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_18646_length_3610_cov_2.726582, whole genome shotgun sequence.
ACCESSION   DAOMAA010000066 DAOMAA010000000
VERSION     DAOMAA010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3610
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18646_length_3610_cov_2.726582"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /locus_tag="PLO59_01500"
     CDS             <1..921
                     /locus_tag="PLO59_01500"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQU99797.1"
                     /translation="VISQAEYDNFKANYDIAKATLEASKQNVAGANYGVKSAEASLKE
                     SVENLNKTSIYAPVDATISKLSKLKGERVVGTNMMDGTEIMRLANLNEMEVIVDVSEN
                     DIVRVHLGDTARIEIDAYSNRKFNGIVTEIANTANNANLLTTDQVTNFTVKVRILRES
                     YMDLIPANNPNISPFRPGMSATIDIETKKVVDAICLPIQAVTTRDTTLKSTTNEKART
                     IKSDSDDAIAENTNKQNLKEVVFVFENGRAKMKTITTGIQDNNFIQILSGVTLKEQVI
                     SGPYNVVAKELSADDKVKVVDKKLLFDVKE"
     gene            1061..1774
                     /gene="tsaB"
                     /locus_tag="PLO59_01505"
     CDS             1061..1774
                     /gene="tsaB"
                     /locus_tag="PLO59_01505"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017258623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HQU99798.1"
                     /translation="MATILHIETATPICSVVLASNKIILSEALTLEPRSHGSQLQAMV
                     QQVLDTAGLTFHNLDAIAISKGPGSYTGLRIGVSAAKGYCYALNIPLIAINTLQAYAI
                     HCINTLRNEQRMPEADNNLPMLFVPMIDARRMEVYTAIFNVDGVAILPTQAMILTTTS
                     FADLLTNNNLVFFGDGATKWQTLAHTFANAFFVETPVSAVGMVQLAMDKLKAKDVENT
                     AYFEPFYLKEFYTPASKKA"
     gene            1928..>3610
                     /locus_tag="PLO59_01510"
     CDS             1928..>3610
                     /locus_tag="PLO59_01510"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HQU99799.1"
                     /translation="MKTKNTKWFLFNLLTLFTFQYSNAQLSLVNTGPTTTPTALVNAI
                     LGGGVQATNITYTGAALSRGRFTSNATNIGMLDGIVLSSGEPNMPAAPASLFKSSAMN
                     NNSDPQLAAIVSPQLVRDASVLEFDFSVASDTVKFEYVFGSEEYNDYVNTSFNDVFGF
                     FINGPGLPPNKNLAIVPGTTNTAVSINNINNGGPFPGLSSGPCTNCAYFVDNVNGSQV
                     FLDAFTTVLQAKTAVQPCELYHIKLAIGDVSDQAFDSDVFIKGSSFASVGLISSFANG
                     VPVGINDTIYGCPGGTITLNLNPASNYLWSTGETTQSIVVNVPPVGAPAQSYSAYVTN
                     PPMYTCFAFTNTSWVVAAPPVANISGNNSFCTGSNTTLTANTGSSYLWSTGATTQSIT
                     VSVAGTFTVTVSNGPGCSATSTPFVVTAGSAVANITGPTAICSGAAANLNANSGISYL
                     WSNGQTTQNTLVNATGTYTVTVTQNGGCTASASQTLNVSSNPVPAITGLNTICAGGVT
                     SFNAGAGFSSYLWSSGQSTQTITPLSAGNYTVTVSNASGCTGTASQSFTINAL"
BASE COUNT         1155 a          704 c          717 g         1034 t
ORIGIN      
        1 gtaatctcgc aagccgagta cgataacttt aaagcaaact acgacattgc caaagcaaca
       61 ttagaagcta gcaaacaaaa tgttgccgga gcaaactatg gcgtaaaaag tgccgaagcc
      121 agtttaaaag aatcggtaga aaacctgaac aaaaccagca tttatgcccc ggttgatgcc
      181 accatctcaa agcttagcaa acttaaaggc gagcgcgttg tgggtaccaa tatgatggat
      241 gggactgaga taatgcgctt ggccaattta aatgaaatgg aagtaattgt agatgtaagc
      301 gaaaacgaca ttgtaagagt acacttaggc gatacagcac gtatagaaat agatgcttac
      361 agtaacagaa aatttaatgg tatagttacc gaaattgcca atacagctaa caatgcaaac
      421 ttgcttacaa ctgatcaggt aaccaatttt accgttaaag tacggatttt acgcgagtct
      481 tacatggatt tgattcctgc taacaacccc aatatatcgc cattcaggcc aggtatgagc
      541 gctaccattg atatagaaac caaaaaagtt gttgatgcca tttgcctgcc catacaagct
      601 gtaaccacac gcgatactac actgaaaagt acaacaaatg aaaaagcacg caccataaaa
      661 tcggattccg atgatgccat agctgaaaac accaacaaac aaaaccttaa agaagttgta
      721 tttgtttttg aaaatggacg cgcaaaaatg aaaaccataa ctacaggcat tcaggataat
      781 aactttattc agatacttag tggcgttacc ctaaaggagc aagttatttc aggcccatat
      841 aacgtagtag ctaaggaatt atcagcagat gataaagtta aggtggttga caaaaagttg
      901 ctttttgatg ttaaagaata aacatgttta aaccaattaa ttggtgtaat agcgttacta
      961 agattctggt ttttaagtaa aaatgtaagg cttattcaat aggtttatag caaccactta
     1021 gttgttagtg cctacataaa attacttttg tgccgttaat atggcaacaa ttttacatat
     1081 cgaaacagct acccctatat gtagtgtagt attagcttct aataaaatta ttttatccga
     1141 agccctgact ttagaaccca ggtctcatgg gtcgcagctt caggcaatgg tgcaacaagt
     1201 gttggataca gcagggctca catttcataa cttagatgca attgcaataa gcaaagggcc
     1261 gggttcatac acgggccttc gtataggagt atcggctgcc aagggctatt gctatgcatt
     1321 aaacatcccc ttaattgcaa taaatacatt gcaagcttat gccatacact gcataaatac
     1381 tttacggaat gaacaaagaa tgcccgaagc tgacaataac ctccccatgc tttttgtgcc
     1441 catgattgat gcgcgtagaa tggaggttta tacagctata tttaatgtgg atggcgttgc
     1501 aattttgcct acacaggcaa tgatattaac cacaacctca tttgctgatt tattaacaaa
     1561 caataatttg gttttttttg gtgatggtgc aactaaatgg caaacactgg cacatacttt
     1621 tgcgaatgct ttttttgttg aaacaccggt ttcggcagta ggtatggttc aattggctat
     1681 ggataaatta aaggcaaaag acgttgaaaa cacagcttat tttgagcctt tttacttgaa
     1741 agagttttat acacctgcat caaaaaaagc ataactaaat aagttagata cttgggtaat
     1801 acggcattaa aaacaaaaac gaaacagcat attttttcaa tgaggtattg ttaataacct
     1861 ctaaaaacgt aagtttgaaa tcggtaagca cccttgtatg ataaaacaga attgatattc
     1921 aatcagaatg aaaacaaaaa acacgaaatg gttccttttt aatctgctca cactttttac
     1981 ttttcaatat agtaatgcgc agcttagttt ggttaacaca gggccaacta caacgccaac
     2041 agcattggtt aatgcaattt taggaggcgg ggttcaggca acaaatataa cttacacggg
     2101 tgctgcctta tcgcgtggcc gttttacctc caatgccaca aatatcggta tgctagatgg
     2161 tattgtactt agttcgggcg aacccaatat gccggcagca ccggcatcgt tatttaagtc
     2221 atcggctatg aacaataact ctgatccaca attagccgct attgtttcac cgcaactggt
     2281 tagagatgcc tcggtattgg agtttgattt ttcagtggct tctgataccg taaagtttga
     2341 atatgttttt ggctccgaag agtataatga ttatgtaaat acttctttta atgatgtctt
     2401 tggcttcttt ataaatggcc cgggtttacc acctaataaa aacttggcca tagtacccgg
     2461 cacaactaat acagcagttt ctattaataa cataaataac ggtggccctt ttccaggttt
     2521 gtcatcaggg ccctgcacta attgtgctta ctttgtagat aatgtaaacg gctcacaagt
     2581 ttttttagat gcattcacaa cggttcttca agccaaaaca gccgttcagc cttgcgaatt
     2641 ataccacatt aaattggcaa ttggcgatgt gtctgatcag gcatttgata gtgatgtttt
     2701 tattaaagga agcagctttg ccagtgtggg cttaatttcg agttttgcta atggtgtacc
     2761 ggtaggtata aacgatacta tttatggctg cccgggagga accataacgc taaacttaaa
     2821 tcctgcatca aattatttgt ggagtacagg cgaaacaaca caaagcattg ttgtaaatgt
     2881 acctccagtt ggcgctccgg cccaatctta ctcggcttat gttactaatc cacccatgta
     2941 cacctgcttt gcctttacca acaccagttg ggttgtagcc gccccaccgg ttgcaaatat
     3001 ttcgggtaat aattcgtttt gtacaggtag taacactaca cttaccgcca atacagggtc
     3061 atcatattta tggagtactg gagctaccac acaaagtata acggttagtg ttgcaggcac
     3121 atttacagtt actgtaagta atgggcctgg ttgtagtgca acatcaacac cttttgttgt
     3181 aacagcaggt agtgcggtag ctaatataac agggccaaca gctatttgta gcggagctgc
     3241 agcaaattta aatgctaact caggcatcag ttatttgtgg agtaatggtc aaaccacaca
     3301 aaatacgctt gtaaatgcaa ccggaaccta tacagtaaca gtaacacaaa atggtggttg
     3361 tacagcaagt gcttcgcaaa cattaaatgt ttcgtccaat ccggtacctg ccataacagg
     3421 ccttaatact atttgtgccg gaggagttac ttcctttaat gcaggtgcag gttttagttc
     3481 ctatttatgg tcttcgggtc aaagtaccca aacaatcacg ccattatcag caggtaatta
     3541 cacagttaca gttagtaatg caagtggttg taccggtaca gcatcgcaaa gtttcactat
     3601 taatgcatta
//
LOCUS       DAOMAA010000067         3573 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_19005_length_3573_cov_3.034963, whole genome shotgun sequence.
ACCESSION   DAOMAA010000067 DAOMAA010000000
VERSION     DAOMAA010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3573
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19005_length_3573_cov_3.034963"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..932
                     /locus_tag="PLO59_01515"
     CDS             <1..932
                     /locus_tag="PLO59_01515"
                     /inference="COORDINATES: protein motif:HMM:NF039614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HQU99800.1"
                     /translation="GSKVIFAKGLFNNIYIRFMSLQTTGTIFMVRNNAFNFNQQTAGS
                     NSFQHAGKADTKEVLAEFDAAVLKINDAGIDVTVFENNNIETPDAIFPNNWIAVTNKH
                     VILFPMMAANRRNERNEMIINHYLKNGRQLLNFTHYESKNLFLEGTGSIVFDHEEKLA
                     FCAISERSNIKVLNDVCQQLSYKAISFETTFHNQPVYHTNVLMSIGNGLAIVCKEIVL
                     PNYHRLIEDALENTTKVIINSGQMQNFAGNVIALIAKNEPCWVMSENAFKSLNKSQLK
                     TFEKSGLLIVVDVGTIEFYGGGGIRCMIAEVFL"
     gene            957..1412
                     /locus_tag="PLO59_01520"
     CDS             957..1412
                     /locus_tag="PLO59_01520"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQU99801.1"
                     /translation="MKKNLLENYTLIEPITAEEFEEYFYKRWLILRKPWNQPAGSEVD
                     MHEVNSIHGMIVDKQQNAVAICRIQMNDSYTAQVRYMAVDEHYRSKGFGKKILSYLEH
                     RAKQRGAKRVYLDARENAVEFYKSCDYRVIKTSYLLFGEIQHFGMEKYL"
     gene            1464..2138
                     /gene="trmD"
                     /locus_tag="PLO59_01525"
     CDS             1464..2138
                     /gene="trmD"
                     /locus_tag="PLO59_01525"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009280620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HQU99802.1"
                     /translation="MRIDIITIHPKLLISPFEHSILKRAQQKKLVNVNIINLRNYANT
                     KHKNTDDYAFGGGAGMVMMIEPIDNCIKALKADNHYDEIIYLSPDGEKLNQSVANQLS
                     LCKNIILLCGHYKGVDERVRQHLITREISIGDYVLTGGELAAAVVADSIIRLLPGVLN
                     DETSALNDSFQDGLVAPPVYTRPAEYNGWQVPEVLLSGNEKVIDDWRHQQSLNRTKIR
                     RPDLLQ"
     gene            2293..2643
                     /gene="rplS"
                     /locus_tag="PLO59_01530"
     CDS             2293..2643
                     /gene="rplS"
                     /locus_tag="PLO59_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HQU99803.1"
                     /translation="MDLLKVAQEASKIEVKPRPAFKAGDTVTVVYKIKEGNKEREQLF
                     QGVVLQRRGNGATETFTIRKISHGVGVERIFPVDSPKIERIDVNKRGIVRRARLFYLR
                     KYSGKAARIKEKKD"
     gene            2811..>3573
                     /gene="recA"
                     /locus_tag="PLO59_01535"
     CDS             2811..>3573
                     /gene="recA"
                     /locus_tag="PLO59_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HQU99804.1"
                     /translation="MASDDAKEKLKALQLSIDKLEKTYGKGTIMRMGDNAIEPIEAIS
                     THSLGLDMALGIGGLPKGRIVEIYGPESSGKTTLAIHAIAEAQLKGGIAAFIDAEHAF
                     DRFYAQKLGVDIENLLISQPDNGEQALEIADNLIRSGAIDIIVIDSVAALTPKSEIEG
                     EMGDSKMGVQARLMSQALRKLAGTINRTGCCCIFINQLREKIGVMFGNPETTPGGNAL
                     KFYASIRLDIRRIGQIKDGDQVSGNRTKVKIVKNKL"
BASE COUNT         1224 a          617 c          700 g         1032 t
ORIGIN      
        1 atggctcaaa agtaatattt gccaaaggtt tattcaataa tatctacata cgttttatga
       61 gtttacaaac aacaggtact atatttatgg tacggaataa tgcatttaac tttaaccagc
      121 aaaccgctgg tagcaatagc tttcaacatg caggcaaagc cgatacgaaa gaagtactgg
      181 ccgaatttga tgccgcagta ttaaaaataa atgatgcggg tattgatgtt accgtttttg
      241 agaataacaa catcgaaacg cccgatgcta tttttccaaa taactggata gctgttacaa
      301 ataagcatgt gattttattt ccgatgatgg ctgccaacag acgtaatgaa cgtaatgaaa
      361 tgatcattaa ccactatttg aaaaacggca ggcaactttt aaactttaca cattatgaaa
      421 gcaagaattt atttttagaa ggtacaggaa gcattgtttt tgatcatgaa gaaaaattag
      481 ccttttgcgc catttctgaa cggagcaaca tcaaagtttt aaatgatgta tgccaacaat
      541 taagctataa ggctattagt ttcgaaacaa catttcataa tcaacctgtt tatcacacca
      601 atgtacttat gagtataggt aacggtttgg ctattgtttg caaagaaatt gtgcttccaa
      661 actatcatag gttaattgaa gatgcattgg aaaatacaac caaggttatt ataaactccg
      721 gccaaatgca aaattttgca ggcaatgtta ttgcccttat agccaaaaac gaaccctgtt
      781 gggtaatgag cgaaaatgct tttaaaagtt taaataaaag ccagttaaaa acattcgaaa
      841 aatcgggtct gcttattgtt gttgatgttg gcaccataga gttttatgga ggaggcggga
      901 tcaggtgtat gatagctgaa gtttttttat agattagcct caccaaattt cagcctatga
      961 aaaaaaactt actcgaaaac tatacgctta tcgaacctat tacagccgaa gaatttgaag
     1021 agtactttta taaacgttgg ctgatattgc gcaagccctg gaatcagcct gccggtagtg
     1081 aagttgacat gcatgaggta aacagtattc atggaatgat agtagataag cagcaaaatg
     1141 cagttgctat ttgccgcatc caaatgaacg atagctacac agcacaggta cgctatatgg
     1201 cagttgatga acattaccga tcaaaaggat ttggcaaaaa aatactaagt tacttagagc
     1261 atagagccaa acaacgtggc gccaaacgtg tttatttaga tgcgcgcgaa aatgcagttg
     1321 agttttataa atcgtgtgat tatagggtta taaaaacttc atacttgctt tttggcgaaa
     1381 ttcagcactt cggcatggaa aaatacttgt aaaaattatc aagtttttaa gtgaaataaa
     1441 tctttgcatt ttaattttgc cgaatgcgaa ttgatataat taccatacac ccaaagctat
     1501 taattagtcc ttttgagcat tcgattttaa aacgtgcgca acaaaaaaaa ttagttaacg
     1561 taaatattat caacttgcgc aattatgcta ataccaaaca caaaaacacc gatgattatg
     1621 catttggtgg cggtgccggt atggtaatga tgattgaacc aattgataat tgcattaagg
     1681 cccttaaagc agataatcat tatgatgaaa ttatctacct cagccctgat ggtgaaaaac
     1741 taaaccaatc agtagccaat caactatcac tttgtaagaa cataatttta ttatgtggtc
     1801 attataaagg tgtggatgag agagtgagac agcatctgat aacgcgtgaa atttcaattg
     1861 gcgattatgt actaaccgga ggcgaattgg ccgctgctgt tgtagccgat agtattatca
     1921 gacttttacc cggtgtgcta aatgacgaaa cctcggctct taacgattca tttcaagatg
     1981 ggctggtggc accacctgtt tacacacgcc ctgccgaata caatggctgg caagttcccg
     2041 aggtattatt atcgggcaat gaaaaagtaa ttgacgattg gcgccaccaa caaagcttaa
     2101 acagaaccaa aatacgcaga cctgatttgc ttcaataaat atttaaccgc cttaattttt
     2161 tcatcgataa aaaagttttt caacaaaatt atttgataaa tatggtgcat taaatcaaca
     2221 tttcaatatc aaaatgttgt catgtttgaa aaaagtgtat gtttgcgccc catttaaaag
     2281 ttaattttca gtatggattt gttaaaagtt gcccaagagg ccagtaaaat tgaagtaaag
     2341 cctcgtccgg ctttcaaagc aggtgatacc gtaaccgttg tttataaaat taaagaaggt
     2401 aataaagaaa gagagcagtt atttcaaggc gttgttttac aacgtagagg taatggagct
     2461 accgaaactt ttactatcag aaaaatatca catggagtag gcgtagagcg catatttcct
     2521 gttgattctc ccaaaataga acgcattgat gtgaataaaa gaggtattgt acgcagggca
     2581 cgtttatttt acttgcgtaa atactcaggc aaagccgccc gcatcaaaga aaaaaaggat
     2641 taaacaattc acctttgttc atgcctttta cttcaaagcc tgcatttatg cgggctttgt
     2701 tgcttttatc caaaagtatt ttgttacaat ttttaggtaa taaccggttg ttccaaatta
     2761 aaattatatc atttttgccc acattgttaa actaaaaaaa taaagaaaat atggcttcag
     2821 acgatgctaa agaaaaactt aaagcgcttc agctttcaat tgataaatta gaaaaaacct
     2881 atggcaaagg tactataatg cgtatgggcg ataacgctat cgaacccatt gaagccataa
     2941 gtacccactc attaggcctc gatatggcac taggcattgg cggcttgccc aagggccgta
     3001 ttgttgaaat ttatggacct gagtcgtcag gaaaaaccac cttagccata catgccatag
     3061 ccgaagcgca acttaaaggt ggcattgctg catttattga tgccgaacat gcattcgata
     3121 ggttttatgc acaaaaatta ggagttgata ttgaaaattt attgatatca caacccgaca
     3181 atggtgaaca ggcacttgaa atcgcagata atttaattcg ctcaggtgca attgatatta
     3241 tcgttattga tagtgtagca gcacttacgc caaaaagtga aattgaaggt gaaatgggcg
     3301 attcgaaaat gggtgtacaa gcacgcctaa tgtcgcaagc attacgtaag cttgccggca
     3361 ccataaaccg gacaggttgc tgctgtattt ttattaacca attgcgcgaa aaaattggtg
     3421 taatgtttgg caaccccgaa acaactccgg gcggaaatgc tttaaagttt tatgcttcta
     3481 taagattaga tatcagacgt attggccaaa taaaagatgg cgaccaggta agtggtaacc
     3541 gaaccaaggt taaaattgta aaaaataaat tag
//
LOCUS       DAOMAA010000068         3548 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_19238_length_3548_cov_2.607214, whole genome shotgun sequence.
ACCESSION   DAOMAA010000068 DAOMAA010000000
VERSION     DAOMAA010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19238_length_3548_cov_2.607214"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="PLO59_01540"
     CDS             <1..682
                     /locus_tag="PLO59_01540"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HQU99805.1"
                     /translation="FRFNFELAGNTLYMYDKITGATPSATTGNYQRFGVDYAQYVRPD
                     IDLRYYQVFSKYAQLVYRLQAGFVRPYGNNTEVLFEKAFFCGGSIDNRAFQPYTIGPG
                     GYKPTTTVQQFGSIKLAFNFEHRFDIFKMLQGAAFIDGGNVWLRNPDPQRPLADFQIN
                     RFYKEFAIGGGLGARLNFNFFIIRLDAAIPIKDPSQPDGQRFVLDQITWGNVISRSTF
                     NLGIGYPF"
     gene            774..3446
                     /locus_tag="PLO59_01545"
     CDS             774..3446
                     /locus_tag="PLO59_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019948684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HQU99806.1"
                     /translation="MKLRIGILFGGTSREREISFAGGRTVYDNLNKNLFEPIPVFIDS
                     FGNMVLLNWQNIYKGSIRDFYPPASQLTHSNFQNYAENLGNLTTHQQLKMLATIGTPV
                     TMEALKDQIDFAFLCLHGADGEDGRMQGALALYRIPYSGSGIFSSAVGMNKALQKELM
                     QKMGFNLPAYATLSRLKWLTIRGNASLLQQEICAKVSLPIVVKAANQGSSIGISVLNT
                     PEVSEWVRAVDKAFFMEEVTYNQWVELHDNAKLHFVRRLTDIREGIGLPIIWDEIIYN
                     ADALLHKLQMHFDVNKTNVLLQAFDSENEIVFESFLEGKEFSCIVIADLNGNPVALPP
                     TEIKKGKELFDYRAKYLPGMARKITPIQLPDAAIYSIMQTCEQLFKQLHFNVYARIDG
                     FIKTDGSIYLNDPNTTSGMLPSSFFFHQAAEIGLNPSQFLSYIIRTSLVARCIDFMQP
                     TLKNLINQFDELLLQEQSSAQNKIKVAVMLGGYSTERHISVESGRNVFEKLASSTKYI
                     PMPVFVSGNAKAHQLHAIPINLLLKDNADDIADKINSYEVHPVIENIKSRCAEITMRF
                     ASAQNVSKPFALTYEALAQQANFVFIALHGRPGEDGDAQQHLEKAGLPYNGSGIKSSQ
                     ITIDKFKTTEILMHAGMHAAKHLMIYKKDWLSDKQKLYNSVIEKFKFPFIAKPVDDGC
                     SSAVKMIRTEEELDAFATLLFRDTAAFDANAANMLKIKLNEELPIKETILIEALIDKE
                     NAVQFLEVTGGMLACKNKAGQIEYEVFEASETLREGDILSLEEKFLAGQGQNITPARY
                     ATNENSNRNISQQVKLALEQAASTLQIEGYCRIDAFVRIYNNEQVEVVFIEVNSLPGM
                     TPATCIFHQAAINNYKPYEFIDKIITYGFAKAGK"
BASE COUNT         1161 a          663 c          678 g         1046 t
ORIGIN      
        1 ctttcgtttc aatttcgagc ttgcgggcaa tacattatac atgtatgata aaattaccgg
       61 agcaacacca tcagccacca ccggcaatta tcagcgtttt ggagttgact atgcacaata
      121 cgtaaggcct gatattgatt taagatatta tcaggttttt agcaagtatg cgcaattggt
      181 atatagatta caagccggtt ttgtgcggcc ttatggcaat aacaccgaag tgcttttcga
      241 aaaagcattt ttttgtggtg gttctattga taaccgtgct ttccaacctt atacaatagg
      301 gcccggagga tacaagccca caactacagt tcaacaattt ggcagcatta agctggcttt
      361 caacttcgag catcggttcg atatttttaa aatgctgcaa ggggctgctt ttattgatgg
      421 tggcaatgtt tggttacgca accccgaccc acaacgccct ttagccgatt ttcaaatcaa
      481 tcgtttttat aaagaatttg ctattggtgg cggattaggc gcacgcctta atttcaattt
      541 ttttattatc agacttgatg ccgctattcc cattaaagac ccatcacaac ctgacggaca
      601 gcgctttgta ttagaccaga ttacatgggg taatgttata agccgcagta cttttaattt
      661 aggcattgga tacccttttt aatttgatta aatagtttaa aaatattttt atctcataaa
      721 tatgcgcact tattaaatac atttttgtgc actattaaat acaagcattt tacatgaaat
      781 tacgtattgg aattttattt ggaggaacat cgcgcgaacg cgaaatatcg tttgcaggag
      841 gccgaactgt ttacgataat ctaaacaaaa atttattcga acccataccg gttttcattg
      901 atagtttcgg taatatggta ctgctcaact ggcaaaatat ttataaaggc agtattaggg
      961 acttttaccc gccagcatcg caacttaccc attcgaactt tcaaaactac gctgaaaatt
     1021 tgggtaacct tactacgcat caacaattaa aaatgcttgc caccattggt acacctgtta
     1081 ccatggaggc gctgaaagac caaatagatt tcgcatttct ttgtttgcac ggtgccgatg
     1141 gcgaggatgg tcggatgcaa ggcgcattgg cattataccg cattccatat tcgggctcgg
     1201 gtatatttag ctcggcagtt ggcatgaata aagccttgca aaaagaatta atgcagaaga
     1261 tgggatttaa tttgcctgct tatgcaacat taagcaggct aaaatggtta actatccgag
     1321 gtaatgcatc ccttttgcaa caagaaatat gcgccaaagt ttcgttgcct atagtagtaa
     1381 aggcagctaa tcaaggtagc agcattggca ttagtgtttt aaatacaccc gaagttagtg
     1441 aatgggttag ggcagtagat aaagcatttt ttatggaaga ggttacctat aaccaatggg
     1501 tagaactcca tgataatgcc aaactacatt ttgtacgcag gcttaccgat atacgcgaag
     1561 gcattggctt acccataatt tgggatgaaa taatttacaa tgccgatgca ttacttcata
     1621 agctgcaaat gcattttgat gtcaataaaa ccaacgtatt actacaagca ttcgattcgg
     1681 aaaacgaaat tgttttcgaa agttttttag aaggcaaaga gtttagttgc attgtgatag
     1741 ccgatttaaa cggcaacccc gttgcactgc cacctactga aataaaaaaa gggaaagaac
     1801 tgttcgacta tcgcgccaaa tacttgccgg gcatggcacg taaaattacg cccattcaat
     1861 tacccgatgc tgcaatttat agcatcatgc aaacctgcga acagcttttt aagcaattac
     1921 attttaatgt gtatgcgcgt attgatggat ttattaaaac tgatggaagc atttatttaa
     1981 atgaccccaa caccacttct ggcatgttgc catcatcatt tttctttcat caggcagccg
     2041 aaattggact gaatccatca cagtttttaa gctacattat cagaacttcg ttagtggcac
     2101 gttgcattga ttttatgcag ccaactttaa aaaatttgat taatcaattt gatgaactac
     2161 tactgcagga acaatcatct gctcaaaata aaattaaagt agcagttatg ttgggcggct
     2221 attcaaccga gcgccatatc agtgtagaaa gtggtcgtaa cgtattcgaa aaacttgcct
     2281 caagtaccaa atatattcca atgcctgtat ttgtaagtgg caatgctaaa gcgcatcagt
     2341 tacatgcaat accaataaat ttgttgttaa aagataatgc cgatgatatt gccgataaaa
     2401 taaatagtta cgaagtacat cccgtaatcg aaaatattaa aagccgatgc gctgaaataa
     2461 ccatgcggtt tgcatctgca caaaacgtaa gtaagccatt tgctttaact tatgaagcat
     2521 tggcacagca agctaatttt gtatttattg ctttacatgg ccgccctggc gaagatggtg
     2581 acgcacaaca acaccttgaa aaagcaggct tgccatataa tggaagtggt ataaaatcat
     2641 cacaaataac cattgataag tttaaaacaa ccgaaatact catgcatgca ggcatgcatg
     2701 ctgccaagca tttaatgatt tataaaaagg actggctaag cgataagcaa aaactttata
     2761 attcggttat cgaaaaattt aaatttcctt ttattgccaa acccgttgat gacggatgta
     2821 gcagtgccgt taaaatgata agaactgaag aagagctgga tgcttttgcc actttgcttt
     2881 tcagagatac ggcagcattt gatgccaatg ccgcaaacat gctgaaaata aaattaaatg
     2941 aagagttacc gattaaagaa accatactta ttgaagcgct tattgataaa gaaaatgcag
     3001 tacagttttt agaagtaacc ggaggtatgt tggcttgcaa aaataaggca ggccaaattg
     3061 aatacgaagt ttttgaagcc agcgaaacgt tacgcgaggg agatatatta agcttagaag
     3121 aaaaattttt agccggccaa ggtcaaaata ttacaccagc ccgttacgct accaatgaaa
     3181 actcaaacag aaatatttcg caacaagtaa aattagcatt agagcaagcc gcaagcacct
     3241 tgcaaataga aggctattgc cgtattgatg catttgtgcg tatttataac aatgaacagg
     3301 ttgaagttgt atttatcgaa gtaaattcat tacccggtat gacaccggcc acttgtatat
     3361 ttcatcaggc agcaataaat aactacaagc cttatgagtt tattgataaa attattactt
     3421 atggttttgc aaaggcaggt aaataataac taccaacatg gcttttaaat aatttttaga
     3481 tttgcccgct caattattac aaacaattta aaatcaaata tttttataaa gaaaaactcc
     3541 atattgat
//
LOCUS       DAOMAA010000069         3533 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_19399_length_3533_cov_2.652674, whole genome shotgun sequence.
ACCESSION   DAOMAA010000069 DAOMAA010000000
VERSION     DAOMAA010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19399_length_3533_cov_2.652674"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3533
                     /locus_tag="PLO59_01550"
     CDS             <1..>3533
                     /locus_tag="PLO59_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99807.1"
                     /translation="CYYFYAQDASSCANEIYYPAFVGGVPQPICFVASSGITFPYCDD
                     FDVNNLWTATSVSGSVWELGAPNYGPTSTAHSAPNAWDIVLTGPYVQGSESYLTSPVF
                     DFAASINCRMSFWYNSATTANFDEDGVYLEYSQAGGPWTLLDVALDPSATNWYNLTNI
                     ANNSQAGWGGNSNGWVQAGFNLSAFDASVGGVQFRFVFKSSQFSTGDGFSLDDFCIIQ
                     PPPTDMGVDQIKQPFATGGGGQNSNAIIRVRNYGTVNVAAFDCYYTIDGGATVVGPTA
                     YAGAPLAPNDTAVITMPTFVIPNGNYDFCAWVVTTGDAYTPNDTMCVASKGIPVIQLT
                     YCNDFDVNGNDWTDVTTPGATTLWELGTPAFGVTSSSHSAPNSWDVNLNSAYTGNANA
                     ILLSPFFDFTPVQNVELSFWINYFTEGFWDGTRLQYSLNAGPWTNLNSDGIAVNWYDG
                     TINSANGDSAWTDDGGGWKKASKNLSQFNNAGNVQFQFVFTSDGIIENDGFSLDDFCI
                     RVPLPDDIGVDAIISPGSNSGANTLTDMTVRVRNFGINTISVFDLYYDIGAGPQGPVA
                     YTGTPLTPNATAIVTIPPQFLPPVGSYCFKAYTVLVGDQDNTNDTTNACSFGVPVDTL
                     NYCENFDGGAIGWQGGSDPSGVNTWELGTPNFGATNTAHSAPNSWDINLNTAYQGMPS
                     NTYAYLYSPFLDFSNATNVNLSFWINFDTENGWDGVRIEYSEAGGPWTILNPVGAVNW
                     YTGNVISGGGNQGWEGNSNGWIKAECNMSQFNNTGLLQLRYAFSCDGIIQNSGFSLDG
                     LCAKIPPPFDAGVTSIDAPAQQGLQIGAPYTLTVTIRNFGQNIISNFPVSYSVNGGGV
                     QTTNYAGSLAPNASATINLLPNFNAITGQFTVCAWTSLTGDGDATNDTTCRDYVGVPT
                     IIPTYTDDFDGPNAGWTTSSANASSKWEYGAPNFGATASAYSPPNCWDVNLNTITTAN
                     ANCELISPYFDFTNVVKGELSFYINYKTDQFDGDGTHVQYSTDGGTNWAVLGAVGSPQ
                     SNNWYTTANIFSSNTPGWSGSGFWMNAFYKDVNGDMDGFNNVRFKFVYTTDGFTTSDG
                     VSIDDIKVYRPIPNTAASVKVEATNGVLQPGNQLLTATLKNSGYIPLQSVSVTLRIDG
                     NIICTDAINPTPVSGGVLFDSTWQHTFSCQW"
BASE COUNT          982 a          687 c          768 g         1096 t
ORIGIN      
        1 ttgctattat ttctatgcgc aagatgcctc atcatgtgct aacgaaattt actaccctgc
       61 atttgtggga ggtgttccac aaccaatttg ttttgtggca tcttcgggta ttacattccc
      121 ttattgtgat gactttgatg taaataattt atggacagcg acttcggttt cgggttcggt
      181 ttgggaatta ggcgcaccta actatggccc aacttcaaca gcacattcgg cacctaatgc
      241 atgggatatt gtattaactg gtccttacgt tcaaggttcg gaaagttatt taacctcacc
      301 cgttttcgat tttgctgcaa gtatcaattg ccgcatgtcg ttttggtaca atagtgctac
      361 tacagcaaat tttgacgagg atggcgtata tttagaatat tcgcaagcag gtggcccctg
      421 gacactttta gatgttgcat tagatccatc agcaactaat tggtacaact taaccaacat
      481 tgccaataac tcacaagcgg gttggggggg taactctaat ggctgggtgc aggcaggctt
      541 taacttaagt gcattcgatg caagtgttgg tggtgtgcaa ttcagattcg tatttaaatc
      601 gagtcagttt agtaccggtg atggtttttc attagacgat ttttgcatca ttcaaccacc
      661 acctacggat atgggtgttg atcaaattaa gcaacctttt gccaccggtg gcgggggaca
      721 aaacagcaat gccattatac gggtacgtaa ttatggaacc gttaacgtag cagctttcga
      781 ctgttactat accattgatg gcggtgccac cgtggttgga ccaacagcgt atgccggtgc
      841 accacttgcg ccaaatgata cggctgttat taccatgcct acatttgtta tacctaatgg
      901 taactacgat ttttgtgcat gggtagttac aactggcgat gcatatacac caaacgatac
      961 aatgtgtgtt gcatctaaag gtattccggt tatacaacta acctattgta acgattttga
     1021 tgttaacggt aatgattgga cagatgttac aacaccgggc gccacaacgc tttgggagtt
     1081 aggtacgcct gcatttggtg ttaccagtag ttcgcatagt gcgcctaata gctgggatgt
     1141 taatttgaat tcggcctata ccggcaatgc aaatgccatt ttattgtcac catttttcga
     1201 ttttacaccg gttcaaaatg tagagctttc tttttggatt aattatttta cggaaggttt
     1261 ttgggatggt acacgtttgc aatattcatt aaatgcaggc ccatggacca atcttaactc
     1321 agatggtata gccgtaaact ggtatgatgg taccattaat tctgccaatg gtgattcagc
     1381 ttggacggac gatggaggcg gatggaaaaa agcatctaaa aacctaagtc agtttaacaa
     1441 tgcaggtaat gtgcagtttc agtttgtatt tacttcagat ggtataatcg agaatgacgg
     1501 cttttcatta gacgacttct gtattcgtgt gccactacct gatgatattg gtgttgatgc
     1561 tataattagc ccgggctcaa attcaggtgc aaatacgtta actgatatga cagttagggt
     1621 gcgtaacttt ggtatcaata caatatctgt attcgatttg tattatgata taggtgctgg
     1681 cccacaaggc ccggtggctt atactggtac accattaaca cccaatgcaa ctgcaattgt
     1741 taccatacca cctcaatttt tacctcctgt cggctcatat tgttttaaag catatacggt
     1801 attagttggc gatcaggaca ataccaacga tacaaccaat gcctgttcat ttggtgtacc
     1861 ggttgacacc ttaaactatt gtgaaaactt tgatggtggt gcaattggtt ggcaaggtgg
     1921 gtctgaccca tctggtgtta atacatggga gttgggtaca cctaattttg gcgctacaaa
     1981 tacagcacat tcggcaccaa attcttggga tatcaattta aatactgcct atcagggtat
     2041 gccaagtaat acgtacgctt acttgtattc accttttctt gattttagta atgcaaccaa
     2101 cgtaaaccta tctttttgga taaattttga tacggaaaac ggctgggacg gtgtacggat
     2161 agaatactca gaagccggtg gcccctggac aatactaaac cctgtaggtg cagttaattg
     2221 gtacacaggt aatgtaattt caggtggtgg caaccaggga tgggaaggaa attcgaacgg
     2281 atggataaaa gccgagtgta atatgagcca gtttaacaat accggcttat tacaactacg
     2341 ctacgctttt tcttgtgatg gcattatcca aaactccgga ttttcattag atggtttatg
     2401 tgctaaaata ccaccacctt tcgatgccgg tgttacttct attgatgcac ccgcgcaaca
     2461 aggcttgcaa attggtgcgc cctatacatt aacagttaca atacgcaact ttggtcaaaa
     2521 tataatcagc aatttcccgg tttcttattc ggtaaatgga ggtggtgtgc aaacaaccaa
     2581 ttatgcaggt tcattggcac ctaatgcttc ggctacgatt aatttattac ctaattttaa
     2641 tgctattaca ggtcaattta cggtttgtgc atggacatcg ttaacaggag atggcgatgc
     2701 caccaacgac actacttgta gagactatgt aggcgtacct acaattattc caacttatac
     2761 cgatgatttt gatggaccca atgccggttg gacaacatca tcggcaaatg caagcagtaa
     2821 atgggaatat ggagcgccta attttggagc cacagcttcg gcttactcgc cacctaattg
     2881 ttgggatgta aaccttaata ccattacaac tgctaatgca aattgtgaat taatatcacc
     2941 ttatttcgat tttaccaatg tggtcaaagg tgagttgtca ttttatatta attacaaaac
     3001 tgatcaattt gatggcgatg gtacacacgt tcaatattcg accgatggag gtacaaactg
     3061 ggcagtttta ggtgctgttg gctcgccaca atcaaataac tggtacacta ccgctaacat
     3121 tttctcaagc aatacaccag gctggagcgg ttcaggtttt tggatgaatg cattttataa
     3181 agatgtaaat ggtgatatgg atggttttaa taatgtgcgc tttaagtttg tttatacaac
     3241 cgatggtttt actacttctg atggtgtatc tattgatgat attaaagtgt atcgtcctat
     3301 acctaataca gcagcatcag taaaagttga agcaactaat ggtgttttac aaccgggcaa
     3361 ccagttatta acagctacac ttaaaaacag tggttacatt ccattacaaa gtgtttcggt
     3421 tacactccgt atagatggta acattatttg taccgatgca ataaatccaa cacctgtttc
     3481 tggtggtgta ttgtttgata gcacctggca acatacattc agttgtcagt gga
//
LOCUS       DAOMAA010000070         3527 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_19454_length_3527_cov_2.623560, whole genome shotgun sequence.
ACCESSION   DAOMAA010000070 DAOMAA010000000
VERSION     DAOMAA010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19454_length_3527_cov_2.623560"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="PLO59_01555"
     CDS             <1..280
                     /locus_tag="PLO59_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011585129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FMN reductase"
                     /protein_id="HQU99808.1"
                     /translation="VVDLLYSEWYHKPVAISTVSDGSFGGSQVITSLQFVLWKMKAIT
                     VPAMFPNPNVKNELNEQGQALPDSPVYKRLSAFIAELIWCIEAVNKMK"
     gene            374..1765
                     /gene="glmM"
                     /locus_tag="PLO59_01560"
     CDS             374..1765
                     /gene="glmM"
                     /locus_tag="PLO59_01560"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HQU99809.1"
                     /translation="MTLIKSISGIRGTIGGQPGNSLTPVDVVKYTAAFATILKNKHNQ
                     TCKVVLGRDARISGLMLENLIGATLISMGIDVINLGLTTTPTTEMAVVHHKAQGGIIL
                     TASHNPKQWNALKLLNEKGEFISAADGEWLLNIAEKGKFDFTDVNSLGKIQKDNDALQ
                     RHITAIINLPMVDAALIGSKKFKVVVDAINSSGAIAVPQLLSALGVEALVINGETNGH
                     FAHNPEPLPEHLHEIAQKVVKESAHAGIVVDPDVDRLAFVCENGEMFGEEYTLVAVAN
                     HMLQYEVGNTVSNLSSTRALRDVTEKAGGKYFAAAVGEVNVVSMMKAQNAIIGGEGNG
                     GIIVPQLHYGRDAMVGIALFLTHLAKTGKSCSMLRSAFPDYHISKNKIELTEDIDVDA
                     VLTYIADRYSQYQVNQIDGVKIDFEKEWIHLRKSNTEPIIRIYAESSSMTTANNLAQK
                     IITDIKEFIQQTA"
     gene            1791..2933
                     /locus_tag="PLO59_01565"
     CDS             1791..2933
                     /locus_tag="PLO59_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010603362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HQU99810.1"
                     /translation="MKVYLDNAATTPVHPDVVATMVPLLQNGFGNPSSIHAFGREIRS
                     TIEQARKKIAQLINCSPGELFFTSGGTEANNMAIRCSIEQLHIKHAITSPTEHHAVLH
                     TLEDLAHQGKIKLSLVKVDAQGNVDLNHLDELLQNNERSLVSLMHANNELGVVLPIEA
                     VGQICKRYDALFHSDTVQTMGHYPFDMKQLQVHFINCAAHKFHGPKGVGFIYINSAIK
                     IAPFITGGSQERNMRGGTENVYGIAGLAKALELAFADMESDTTYIRSLKDYMQSKLVE
                     TIPGIKFNGDISANSLYTVLNVSFPPADFAEMMLFNLDIKGIAASGGSACSSGSNVGS
                     HVLGAIGVDPSRPCVRFSFSKLNTKAEIDYTIEVLKGLYKNQSATA"
     gene            2955..>3527
                     /locus_tag="PLO59_01570"
     CDS             2955..>3527
                     /locus_tag="PLO59_01570"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HQU99811.1"
                     /translation="MLLEVNPLNPDSRQINKIVACLKDDGIIVIPTDTVYAMGCSMHS
                     SKAFEKICKLKNIRPEKANFSFIMNSLSDISAYTKPFDRSVFKLLNKSLPGPFTFILE
                     ASNNIPSFFRNKKKTVGIRIPNHKVPIEIANQLSNPLLVTSLHLTPESNSYLDYPLNP
                     LEIFEQYSSLVDIIIDGGWGHTIPSTVVDCT"
BASE COUNT         1148 a          644 c          724 g         1011 t
ORIGIN      
        1 tgttgttgat ttgctttata gtgagtggta tcataaaccg gtagccataa gcacggtttc
       61 cgatggctca tttggaggct cgcaggtaat taccagtttg caatttgtgt tatggaaaat
      121 gaaggccata accgtaccgg ccatgtttcc aaatcccaac gtgaaaaacg agctaaacga
      181 acaaggtcag gcactgcccg atagtcctgt ttacaagcgt ttaagcgctt ttattgccga
      241 gctgatatgg tgcattgagg ctgttaacaa aatgaaatga tacataactt ttatgcatag
      301 gtaattgcct tatatttgtg ggctttttta tacggcattt caaagtttta tcaatcattt
      361 ataaaaatat aaagtgactt taattaaatc aatctctggt atcagaggta ccattggcgg
      421 gcaacccggt aactcgctaa caccggttga tgttgtaaaa tatacagctg cttttgcaac
      481 catccttaaa aataaacata accaaacttg caaggtagtt ttgggccgcg atgcccgtat
      541 cagtgggctg atgttagaaa atttaattgg tgccacactt attagcatgg ggattgatgt
      601 aataaattta ggattaacca ctacgcccac tacagaaatg gctgtagtgc atcacaaggc
      661 acaaggtggt attatactta cagccagcca taaccccaaa caatggaatg ccttaaaact
      721 tctgaacgaa aaaggtgaat ttatttctgc agccgatggt gaatggcttc ttaacattgc
      781 cgaaaaaggc aagtttgatt ttacagatgt aaatagctta ggtaaaattc aaaaagataa
      841 cgatgctttg caacgccaca ttacagccat aattaactta cctatggttg atgcagcatt
      901 aattggatcg aaaaagttta aagttgtggt tgatgcaata aactcatcag gcgctattgc
      961 agtaccgcag ctcttaagtg cattaggcgt agaggcattg gtaattaatg gcgaaacaaa
     1021 tggccatttt gcacacaacc ccgaaccatt gcctgaacat ttgcatgaaa tagcgcaaaa
     1081 agttgtaaaa gaaagtgccc atgccggtat tgtagttgac cctgatgtgg accgtttggc
     1141 atttgtttgt gaaaatggcg aaatgtttgg cgaagaatat acacttgttg ccgttgccaa
     1201 tcacatgtta caatatgaag taggcaatac ggtaagcaac ttatcaagta cacgcgcact
     1261 tagagacgtt accgaaaaag cgggaggtaa atactttgcc gctgcagttg gcgaagttaa
     1321 tgtggtaagt atgatgaaag cacaaaatgc aattattgga ggcgagggta atggtggtat
     1381 tattgtgcct caattacact acggacgtga tgcaatggta ggtatagcct tattcctaac
     1441 acatttggca aaaacaggca aaagttgcag catgttgcgc tcagcatttc ccgattacca
     1501 tatatcgaaa aataaaattg aattaaccga agatattgat gtagatgccg tgctaacata
     1561 catagccgac cgttacagcc aatatcaggt taaccagatt gatggcgtta aaattgactt
     1621 tgaaaaagaa tggattcatt tgcgcaaatc caataccgaa cccattatac gcatttatgc
     1681 tgaaagcagc agtatgacta cagccaacaa tttggcacaa aaaattatta cagacataaa
     1741 agagtttatt cagcaaactg cctgaacgaa ttataaatca tcacatcaat atgaaagtat
     1801 atttagataa tgctgccact acacctgtgc atccggatgt agtggcaacc atggttccat
     1861 tattacaaaa tggatttggc aatccatcat ctatacatgc ctttgggcgc gaaatacgct
     1921 ccaccattga acaggcgcgt aaaaaaattg cgcagttaat taactgcagt ccgggcgaat
     1981 tgttttttac ttcaggcggt accgaagcta ataatatggc aatcaggtgt tccatcgaac
     2041 agttgcatat taagcatgca attacatcgc caaccgaaca tcatgctgta ttgcatacgc
     2101 tcgaagattt agcacatcag ggcaaaataa aactgagttt ggtaaaagtg gatgctcagg
     2161 gcaatgtgga tttgaatcat ttagacgaac tgttacaaaa taacgaacgc agtttggtat
     2221 cgctaatgca tgcaaacaat gaactgggtg tagtgttacc aattgaagcg gtaggccaaa
     2281 tttgtaagag gtatgatgca ttatttcata gtgatacggt gcaaaccatg gggcattatc
     2341 cttttgatat gaaacagttg caggttcatt ttattaattg tgcagcacat aaatttcacg
     2401 gcccaaaagg cgtaggtttt atatacatta acagtgcaat taaaattgca ccatttatta
     2461 ccggaggctc gcaggaacga aatatgcgag gcggtaccga aaatgtgtat ggcatagccg
     2521 gattggctaa agctttagag ttggccttcg ctgatatgga atcagacact acctatatac
     2581 gtagtttaaa agattatatg caaagtaaat tagtagaaac catacctggt attaaattta
     2641 atggcgatat aagtgccaat agtttatata cggttcttaa tgtttcgttt cccccggccg
     2701 actttgccga aatgatgtta tttaatctcg atatcaaagg catagcagca tcgggtggaa
     2761 gcgcctgttc atcgggtagt aatgtaggat cgcatgtatt aggtgcaatt ggagttgacc
     2821 cttcacgtcc ttgtgttcgc ttttcattca gtaaattaaa taccaaagca gaaattgact
     2881 ataccatcga agttttaaaa ggactttata agaatcaatc ggcaactgcc tgattaaaaa
     2941 atcctgaaaa ctgtatgctt ttagaagtta atcctttaaa ccccgatagt cgccaaatta
     3001 ataagatagt ggcttgttta aaggacgatg gtattattgt tatcccaact gatacagtat
     3061 atgcaatggg ctgtagcatg catagcagta aagcatttga aaaaatatgc aaactgaaaa
     3121 acatacgacc cgaaaaagca aatttttcat ttatcatgaa tagcttgtct gatatttctg
     3181 cttatacaaa accattcgat cgttctgttt ttaaactatt aaataaatcg cttccggggc
     3241 cgtttacatt tattctggaa gccagcaata acatcccatc attttttaga aataaaaaga
     3301 aaacagtggg aattcgcata ccaaatcata aagtacctat tgaaattgcc aaccaattaa
     3361 gcaatccatt attggtaaca tcattgcacc tgacacctga aagtaatagc tatctggatt
     3421 acccgcttaa tccattagaa atatttgagc aatacagtag tttggttgat ataattattg
     3481 atggtggctg ggggcatacc ataccaagta cagttgttga ttgtact
//
LOCUS       DAOMAA010000071         3524 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_19481_length_3524_cov_3.842606, whole genome shotgun sequence.
ACCESSION   DAOMAA010000071 DAOMAA010000000
VERSION     DAOMAA010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19481_length_3524_cov_3.842606"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="PLO59_01575"
     CDS             complement(<1..535)
                     /locus_tag="PLO59_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002975303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
                     /protein_id="HQU99812.1"
                     /translation="MPKQKSITEKQQKDLLEILHKRFIKNMSRHKNLEWQQVADKIVL
                     VKDKMWSLYEMENTGGEPDVVDYIAKDNTFLFFDCATESPAGRRSLAYDKAGQESRKE
                     FKPVNNAIDVAASMGINLLNESQYRFLQTLGKFDCKTSSWIITPESIRKLGGALFGDY
                     RYDSVFIYHNGAQSYYAA"
     gene            complement(637..1701)
                     /locus_tag="PLO59_01580"
     CDS             complement(637..1701)
                     /locus_tag="PLO59_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HQU99813.1"
                     /translation="MRDDNNAATVSKTDTSEICCESNLPSRFGKQGSTTNIVPSGDTS
                     TQGMVWINGGTFMMGADNDQASRDEYPKHRVTVDGFWMDATEVTNAQFDAFIKATGYI
                     TTAEVKPLWEDLKKQLPPNTPKPHDSLLVAASLVFKPSNQPVDLNYFSQWWQWLAGAN
                     WKHPQGPNSNIKDKENYPVVHISWDDAVAYCKWAGKRLPTEAEWEYASRGGLVNNIYS
                     WGNEHVDAGKPKCNSWQGKFPYRNDLSDNYYGAAPVKSYTANGYGLFDMAGNVWEWCS
                     DNYKDSYYTEVQKGAINPQGPETSFDPEEPYTPKRVTRGGSFLCNDGYCSGYRVARRM
                     KSSPDTGLEHLGFRCVRDKR"
     gene            complement(1868..2419)
                     /locus_tag="PLO59_01585"
     CDS             complement(1868..2419)
                     /locus_tag="PLO59_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99814.1"
                     /translation="MKHNIFYFIILISHILFFKSIAQHQPAHDFKPHHSISLVLSHAT
                     VFNGLNTEGKRQVINLPAWGIDYNYQFKPKWSLGIHTDIIIEKFVVEKQGETGTEAAL
                     ERSYPIAPAIVAMYKINHHWSGLLGAGIEFEKTENFFLNRLGIEYALEMPNEWEFLAS
                     FNYDIKWNAYDTYILGVGVAKQF"
     gene            complement(2771..3427)
                     /locus_tag="PLO59_01590"
     CDS             complement(2771..3427)
                     /locus_tag="PLO59_01590"
                     /inference="COORDINATES: protein motif:HMM:NF016207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HQU99815.1"
                     /translation="MYHFSYYKETEQQVIIDFMEEHPFAFLTGSFLSGQQVATQIPVL
                     IEQRGDELFVQGHIMRNTDHHKAFIENPNALIVFTGPNCYVSASWYSNQQSGSTWNYM
                     SVHLTGQITFMQPQAFADFMRRFTLKFENGNQQSPTIFDNLPADYIDKMMPGIAGFEM
                     KAQKVDNVFKLSQNRDAESYGNIIAQLRAKGGNSALIAEAMQKREAKLFSNNNGKPKL
                     "
BASE COUNT         1032 a          700 c          620 g         1172 t
ORIGIN      
        1 gtgctgcata gtacgattgt gcgccattgt gataaataaa aacgctatca tatctataat
       61 caccaaataa ggctcctcct aactttctga tgctttcagg cgtaattatc caactggatg
      121 ttttgcaatc aaatttaccc agtgtttgta agaaacgata ttgactttcg ttcaacaaat
      181 taatacccat tgacgcggcc acgtcaatag cattgtttac aggtttaaat tctttgcgcg
      241 attcctgccc tgctttatca taagccaaac ttctgcgacc tgcggggctt tcggtagcac
      301 aatcgaaaaa caaaaatgtg ttgtcttttg caatgtaatc aaccacatcc ggttcgccac
      361 cggtattttc catttcatat aacgaccaca ttttgtcttt aactaaaaca attttatccg
      421 ccacttgttg ccactccaaa tttttatgac ggctcatgtt ttttataaat cgtttatgta
      481 gtatttcgag caagtctttc tgttgctttt cagttattga tttttgtttg ggcataattc
      541 tataaccttg gtttctgttt taacttatgt atgtgctgtt taaattataa tgtcagaagg
      601 tgtgatatgg cttattgttt aatgcttaaa gaaccgctac cttttatctc gcacacatct
      661 gaaacccaaa tgttccaaac ctgtatcggg gctgctcttc atcctgcgtg ccacgcggta
      721 tccgctacag tatccatcat tacataaaaa cgaaccgcca cgcgttacgc gtttgggtgt
      781 ataaggttct tcggggtcga aactggtttc ggggccttgt ggattaatag ctcctttttg
      841 tacttcggtg tagtagctgt ctttataatt atcgctacac cactcccaaa cattgccagc
      901 catatcaaat aaaccatacc catttgctgt gtatgattta acaggggctg caccataata
      961 gttatcgctt aaatcgttgc gatacggaaa tttgccttgc cacgaattgc acttaggctt
     1021 gcctgcatct acatgctcat tgccccaact ataaatatta tttactaagc ctccacggct
     1081 tgcatactcc cattcggctt cggttggcaa tcgtttgccg gcccatttac aataagcaac
     1141 ggcatcatcc caacttatat gtactaccgg ataattttct ttgtctttaa tattactgtt
     1201 tggcccttgt ggatgtttcc agttagcacc tgccaaccat tgccaccact ggctgaaata
     1261 atttaaatca accggttggt ttgaaggctt aaacacaagc gatgcagcca ccaataaact
     1321 atcgtgcggt ttgggtgtgt tgggtggcag ttgttttttt aaatcttccc aaagcggttt
     1381 tacttctgct gtagttatgt atccggttgc ttttataaaa gcatcaaatt gtgcattggt
     1441 aacttcggtg gcatccatcc aaaagccatc aaccgttaca cgatgtttgg gatattcatc
     1501 gcgcgatgcc tgatcattat cagctcccat cataaaggta cctccattaa tccaaaccat
     1561 accttgtgtt gatgtatcgc ctgatggaac aatattggtg gttgaacctt gcttgccaaa
     1621 gcgcgatggc aaatttgatt cgcaacaaat ttccgaagta tctgtttttg aaacggtggc
     1681 cgcattatta tcatcccgca tttgtttgca tgatacaaat gtggctgcaa tacaaaaaat
     1741 ggttatgtag cgttgcttaa acatggcata gcaagaaaaa attaaattaa tagcaagagt
     1801 aaaaaaattt gacgtgcatt taatgaatca gataatgtaa tagatgaatg ctacttcaaa
     1861 caaccattta aaattgtttt gcaacaccaa cacctaaaat gtaagtgtca taagcattcc
     1921 atttgatatc ataattaaat gaagctaaaa actcccattc gttgggcatc tcaagggcat
     1981 actcaatacc caaacgattc aggaaaaaat tttctgtttt ctcaaactca attcctgcac
     2041 ctaataaacc actccaatgg tggttaattt tatacatggc tacaatagcc ggtgcaatgg
     2101 ggtagctacg ttctaatgct gcttcggttc ctgtttcacc ctgtttttcg acaacaaatt
     2161 tttcgataat aatatcagta tgaataccca aactccattt gggcttaaac tgatagttgt
     2221 aatcaatacc ccaagcgggc aaatttataa cctggcgttt gccttccgta ttaagcccat
     2281 taaaaacagt tgcatgactt aataccaaac ttatgctgtg atgcggttta aaatcatgcg
     2341 ctggttggtg ttgcgcaata cttttaaaaa ataaaatgtg gctaattaaa ataataaaat
     2401 aaaagatgtt gtgtttcatt ttttagaaaa cttcatgggc gattaaaaag taaagaatta
     2461 aaaaaataca aacgaatacc aaggccgttt caactgcaaa aatttaaaat acgtaaacaa
     2521 aaaacaaagt ttaactgtat aattttaaaa gtaactcttt ggttgttaaa aaaattcgtt
     2581 tcaagtatca tgcagttaca ttaataaata aaatgtctaa tatggcaaat tttgagcaag
     2641 gctgtatatt gctgatgtag gtaactgcac aattgcatga actgcataag ttgccacttg
     2701 ccaaaaaaaa tttctgctgc gcgtattaaa attattttca gatgcttgac tggctaaatg
     2761 tacaacggta ttataatttg ggtttgccgt tgttgtttga aaaaagtttt gcttctcttt
     2821 tttgcatcgc ttcagcaatt aatgcgctgt tgccgccctt tgcccgaagt tgggcaatta
     2881 tgttgccata gctttctgca tctctatttt gactgagctt aaaaacattg tcaacctttt
     2941 gggctttcat ttcgaaaccg gcaatgcccg gcatcatttt atcaatataa tctgccggca
     3001 aattatcaaa tatagtaggc gattgttgat taccgttttc aaactttaaa gtaaaccttc
     3061 gcataaaatc ggcaaatgct tgtggctgca taaatgtaat ttgacctgtc aggtgcacgc
     3121 tcatataatt ccaggttgag cccgattgct gattgctgta ccaggacgca cttacatagc
     3181 agtttggacc ggtaaaaact attaatgcat ttggattttc gataaaggct ttgtggtggt
     3241 cggtattgcg catgatatgc ccttgaacaa acagttcatc acctctttgt tctattaaca
     3301 ccggtatttg tgttgcaact tgttgacctg ataaaaaact tccggttaaa aatgcaaatg
     3361 gatgttcttc cataaagtca attattacct gttgttcggt ttctttataa taggaaaaat
     3421 gatacatgtt ttatattgtt tttttaatgt attttaaaaa tacgtatttg tttagcttac
     3481 ctatttactt tttataaaat cccatttcat ccatataaac aaat
//
LOCUS       DAOMAA010000072         3494 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_19771_length_3494_cov_2.414655, whole genome shotgun sequence.
ACCESSION   DAOMAA010000072 DAOMAA010000000
VERSION     DAOMAA010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19771_length_3494_cov_2.414655"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..573
                     /locus_tag="PLO59_01595"
     CDS             97..573
                     /locus_tag="PLO59_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HQU99816.1"
                     /translation="MKKILLSLTLFTCFSVAAIAQDEVAKAAPANPNAPEISFEEELH
                     DFGTLPFGGNGNFDFKFTNTGKEPLVISNAKGSCGCTVPKWPKEPIMKGQTGSINVNY
                     DTKRVGPFTKTVTINSNAKTASKTITIKGNVMSQEETENQSPIKKNSSFSPLEKTN"
     gene            609..1073
                     /locus_tag="PLO59_01600"
     CDS             609..1073
                     /locus_tag="PLO59_01600"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HQU99817.1"
                     /translation="MKKSILLSATFSMVLLLSNTSSTLAQDAKVADTKALENKNQADF
                     KFDALEYNYGSIKQGESVSYSYKFTNVGKEPLVITSAHGSCGCTVPDWPKEPIKKGET
                     GIIKVTFNSAGKMGAQDKTVTINSNAKVSPVVLHIKGNVEAPAQPAAGGDAH"
     gene            1148..2344
                     /locus_tag="PLO59_01605"
     CDS             1148..2344
                     /locus_tag="PLO59_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003001108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HQU99818.1"
                     /translation="MPTISNKGLQMPASPIRKLVPFAESAKKKGIKIYHLNIGQPDIK
                     TPDTMINAMHQLNLKVIEYSHSAGIESYRKRLTEYYNRYNIALNYTDIMITTGGSEAI
                     EIAMMTCLNEGDEIIVTEPFYANYNGFSMQAGVKVVPITASIENGFALPAISEFEKVI
                     TKRTKAILICNPNNPTGYLYTRAELEALREIVLKHDLFLFSDEVYREFCYDAEYISAM
                     HLQGLDKHVIMVDSVSKRYSACGARIGALISKNEEVMQTALKFGQARLSPPTFGQIAA
                     EAAIDTPDSYFKEVKDEYVARRNYVVDALNKIPGVFCPMPKGAFYCIAQLPIDDCDKF
                     CQWLLEEFSYDNQTVMLAPATGFYSTPGFGKNQVRIAYVLNVESLKNAMACLQKALEV
                     YPGKTN"
     gene            complement(2349..2951)
                     /locus_tag="PLO59_01610"
     CDS             complement(2349..2951)
                     /locus_tag="PLO59_01610"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HQU99819.1"
                     /translation="MYQIFNNEKCLHIVKHKPKQYSKSSILINANDSEAINEAVQNLL
                     NEKPNSIFLVTPFIHKVFKEVRAYFLIIEAAGGLVYNDANKLLMIYRLGKWDLPKGKI
                     ERGEAKRDAAIREVQEECALTQLRIESFIATTYHIYFIKNQPVLKFSHWYKMYCADKA
                     IPQPQIEEGISEVIWANKKQVAKNAIASYATIKWLLQNKI"
     gene            3029..>3494
                     /gene="pyrE"
                     /locus_tag="PLO59_01615"
     CDS             3029..>3494
                     /gene="pyrE"
                     /locus_tag="PLO59_01615"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015482307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HQU99820.1"
                     /translation="MERDTAKLIADHLLQIKAVKLQPNEPYTWASGWKSPIYCDNRIT
                     LSYPRIRTFIRQELAKLIENKFGRPDLIAGVATAAIAQGALVAEALGLPFCYVRPKSK
                     EHGLKNQIEGEVSPNQSCVVIEDLISTGSSSLQAVELLKAEGVVVKGMVSIFT"
BASE COUNT         1175 a          630 c          651 g         1038 t
ORIGIN      
        1 ttatagatgc ataagcataa gattatcctt tcattgtaag ttcattgtgc caaaaatact
       61 tttgaaccgt tcaatacaaa cacataacaa acaactatga aaaaaatatt attaagccta
      121 acacttttta catgtttttc agttgcagct atcgcacagg atgaagtagc aaaagcggca
      181 ccggcaaacc caaatgcacc tgaaatttct tttgaagagg agttacatga ttttgggaca
      241 ttaccttttg gcggcaatgg aaactttgat tttaaattta ccaacacagg taaagaaccg
      301 ttggttatca gtaatgcaaa aggttcgtgt ggatgtacgg tacctaaatg gccgaaagag
      361 ccgattatga aaggacaaac cggttctatc aacgttaatt atgatacaaa gagagttggg
      421 ccatttacaa aaacagtaac cataaacagt aatgcaaaaa cggccagtaa aaccattacg
      481 ataaagggaa atgtaatgag ccaggaagaa accgaaaacc aatcgccaat aaagaaaaac
      541 agtagttttt cgccattaga aaaaactaat taacttatca taaaaattaa taattaaaaa
      601 tcagaaaaat gaaaaaatcc attttattat ccgcaacatt ttcaatggtg ttgttgctgt
      661 cgaacacctc cagcacctta gctcaggatg caaaagttgc agataccaaa gccctagaaa
      721 ataaaaatca ggctgacttt aaattcgatg ctttagaata caattatgga agcattaagc
      781 aaggcgaatc ggttagttat agttacaaat ttaccaatgt tggtaaagaa ccattagtaa
      841 ttacaagtgc acatggttca tgtggttgta cggtacctga ttggccaaaa gaacccatca
      901 aaaaaggcga aaccggcatt ataaaagtta catttaactc ggcaggcaaa atgggagcac
      961 aggataaaac ggttaccatt aacagtaatg caaaggtaag tcctgtagta ttgcacatta
     1021 aaggaaatgt agaagcaccg gcacaacctg ctgcaggtgg cgatgctcat taatttgatt
     1081 gcataaataa tttatgtttg cgcccacaga ttatctgtgg gcgttttcat tttaaatcaa
     1141 acctcttatg ccaaccatat caaataaagg ccttcaaatg ccggcctctc caattcgtaa
     1201 attagtaccc tttgcagaat ctgctaaaaa gaaaggcatt aaaatttatc atttaaatat
     1261 tgggcaaccc gatataaaaa cgcctgatac tatgataaat gctatgcatc aactcaattt
     1321 aaaagttatt gagtatagcc atagtgcagg tattgaatcg tatcgcaagc gtttaaccga
     1381 atattataac cgttacaaca ttgcattaaa ttatacggat ataatgatta caaccggtgg
     1441 ctctgaagcc attgaaattg ccatgatgac ttgcttaaac gaaggtgatg aaataattgt
     1501 aaccgagcca ttttatgcaa actataatgg ttttagtatg caggccggtg taaaggttgt
     1561 tcctattacc gcttctattg aaaatggctt tgcattgcct gcaattagtg agtttgaaaa
     1621 ggtaattacc aaacgtacaa aggccatttt aatttgtaat cccaataatc caacaggcta
     1681 tttatataca cgtgccgagt tagaagcttt acgcgaaata gttttaaaac acgatttgtt
     1741 tttatttagc gatgaagtgt atcgtgaatt ttgctatgat gctgaataca ttagtgccat
     1801 gcacttgcaa ggcttggata agcatgtgat aatggttgat agtgttagca aacgatatag
     1861 tgcatgtggt gcgcgcattg gtgcattaat cagcaagaat gaagaagtta tgcaaacagc
     1921 attaaagttt ggacaggcaa gacttagtcc gccaactttt ggtcaaattg cagccgaagc
     1981 agctattgat acacctgata gttattttaa agaagtaaag gatgaatatg ttgcacgcag
     2041 aaattatgtt gttgatgctt taaataaaat tccgggtgtg ttttgcccaa tgcccaaagg
     2101 tgcattttat tgcattgcac aactacctat tgacgattgc gataagtttt gccaatggct
     2161 tctggaagaa ttcagttacg ataatcaaac cgtaatgttg gcacccgcta ccggctttta
     2221 cagtacacca ggttttggaa aaaaccaagt gcgtatagcg tatgtgttaa atgtcgaatc
     2281 gttaaaaaac gctatggctt gtttgcaaaa agcattggaa gtttatcccg gtaaaacaaa
     2341 ttaattggtc atattttatt ttgcaatagc catttaatag tagcataact agcaattgca
     2401 tttttagcaa cttgtttctt gttagcccaa ataacttcac tgattccttc ttcaatctgc
     2461 ggttgcggaa tagctttatc agcgcaatac attttatacc agtggctaaa ttttaaaacc
     2521 ggctgatttt ttataaaata tatatgatag gtcgtagcta taaatgattc aattcttaat
     2581 tgcgtcaatg cacattcctc ctgcacttct cttattgctg catcacgttt tgcctctccc
     2641 ctttctattt tacctttcgg caaatcccat ttgcctaatc tgtaaatcat caaaagtttg
     2701 ttggcatcgt tataaaccaa acctccggca gcttcaatga ttaaaaaata tgcacgcact
     2761 tccttaaaca ctttatgaat aaagggagtt accagaaaaa tgctgttggg tttttcgttt
     2821 aacagatttt gcacggcctc attaattgct tcgctatcat ttgcattgat aagtatgctt
     2881 gacttgctat attgttttgg tttatgtttg actatgtgta aacatttttc attattaaaa
     2941 atctgataca ttgcacaaaa ttttaaaaag tcaaagcccg aattaagcaa caataaaatg
     3001 attaattagc ctcacccaaa cagaaattat ggaaagagat accgccaaat taattgccga
     3061 ccatttactt caaataaaag ctgtaaagct acaacctaac gaaccataca catgggccag
     3121 tggctggaaa tcgcccattt attgtgataa cagaataaca ctttcgtatc cccgtatccg
     3181 aacttttata aggcaggagc tagccaaatt aattgaaaac aaatttgggc ggcccgactt
     3241 aattgcaggt gtagctacag cggctattgc acaaggcgca ttagttgccg aagcgctggg
     3301 ccttccgttt tgctacgtta gacctaaatc aaaagagcat ggtttaaaaa atcaaatcga
     3361 aggcgaagta agtcccaatc aaagttgcgt ggttattgaa gatttaattt cgaccggcag
     3421 cagtagctta caagccgttg aattacttaa agctgagggc gtagttgtaa aaggtatggt
     3481 tagtattttt actt
//
LOCUS       DAOMAA010000073         3492 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_19789_length_3492_cov_3.479197, whole genome shotgun sequence.
ACCESSION   DAOMAA010000073 DAOMAA010000000
VERSION     DAOMAA010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19789_length_3492_cov_3.479197"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="PLO59_01620"
     CDS             complement(<1..555)
                     /locus_tag="PLO59_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012924645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HQU99821.1"
                     /translation="MIQFDKEILSNGLTVLVHQDFSSPLVAMNILYKAGARDEHPDKT
                     GFAHLFEHLMFGGSINIPSYDEPLQKAGGDNNAFTSNDFTNYYLTIPANNIETAFWLE
                     SDRMLSLAFNEKSLEVQRNVVVEEFKQRYLNQPYGDAWLQLRPLAYKVHPYQWATIGK
                     DISHIENASLDDVKMFFNKYYLPNN"
     gene            complement(585..1274)
                     /locus_tag="PLO59_01625"
     CDS             complement(585..1274)
                     /locus_tag="PLO59_01625"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HQU99822.1"
                     /translation="MHLFYSNNIIDKLATLDDDECKHALQVLRMRIGDTAQVFDGMGN
                     LYDAVFIKNQDKQLIAQVTFKYQLQPLPYNLHIAIAPTKQTDRFEWFVEKAIEMGVHE
                     ITPVICHDSVRLNYKTERIHKIKLAACKQAFQLYLPVINEPVHFVDFIKKHQAGFIAH
                     CKPENKNSLANLINRQSRVIMLIGPEGDFTSHEVELALQHNYKPVSLGNNRLRTETAG
                     IYISAYMRSLI"
     gene            1594..2208
                     /locus_tag="PLO59_01630"
     CDS             1594..2208
                     /locus_tag="PLO59_01630"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HQU99823.1"
                     /translation="MKKTLLVLSMVALSSVAMAQKGSWYAGGMVGFGSSTDKDANDKK
                     NVTSTWNFSPEVGTFLRDDIQLGLVLGLGGSKTKFDGDETGSTSRFSPTVYGRKFFKV
                     TEQFSMFGGLYLSLINEKSTDKSSGYEIEMKSNGFGAKVGVGCAYALSPRFTAVGQFG
                     VLNFQSVSNEVEGNDAGKSSDFGLDINTFGAAAFNVGLYYTFMQ"
     gene            complement(2275..>3492)
                     /locus_tag="PLO59_01635"
     CDS             complement(2275..>3492)
                     /locus_tag="PLO59_01635"
                     /inference="COORDINATES: protein motif:HMM:NF019205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HQU99824.1"
                     /translation="FHDLAIKTHQQVKNEQVVASYNIYGSGSNGNDSWFKIQVKPVIN
                     NEVVTGIILISQIITEQYKNDEANAHFLSQLEATIESTADGILLVDKLGRVSLYNKKF
                     LELWRIPPEILELRDDNSTLQYVVNQLKYPDDFLAKVQWLYDKPDEICFDVIEFIDGR
                     VFERYSQAQLINGSYAGRVWSFRDITSRLQAEEAIKQSLQEKEVLLKEVHHRVKNNLQ
                     IISSIINLQAANINDPQTLEAMRDSQSRIRCMGYIHELLYQNKDFSSINFKEYISSIA
                     NSLLFTYSIGRQIQLKLSVQEIHLNIDVAIACGLIVNELLTNSLKYAFTIEHRNKYIE
                     VTAAELDGKVVLTVADNGKGLEAGFDINNYETLGMQLVSGLVEQIDGALRVINKNGVL
                     FEIEFFMTSKLQH"
BASE COUNT         1096 a          627 c          611 g         1158 t
ORIGIN      
        1 attattaggc aaataatatt tattaaaaaa catttttaca tcatccagtg atgcattttc
       61 gatatgacta atgtctttgc caatagttgc ccattggtaa ggatgtactt tataagctaa
      121 tggtcgcaat tgcaaccagg catcgccata aggctgattt aaatagcgtt gcttaaactc
      181 ttcgacaaca acattacgtt gcacctctaa acttttttca ttaaaagcta agctaagcat
      241 cctgtcgctt tcgagccaga aagcggtttc gatattattg gcaggtatgg ttaaataata
      301 gttggtaaag tcgttgctgg taaatgcatt gttatcgcca ccggcttttt gtaaaggctc
      361 gtcatagctt ggaatgttta tcgagccacc aaacatcaaa tgttcgaaca gatgtgcaaa
      421 accggtttta tcgggatgct catcgcgcgc accggctttg tataaaatat tcatggctac
      481 taaaggcgat gaaaaatcct gatggactaa aacggttaat ccattactta aaatctcttt
      541 atcaaattga atcatgtgat atttaaaaat tatcagagtg tttattaaat caaactacgc
      601 atatatgcac tgatataaat accagccgtt tcggttctta gcctgttatt gcctaatgaa
      661 accggtttat aattgtgttg caatgctaat tcaacttcat gggaagtaaa atcgccctcg
      721 gggccaataa gcataattac ccggctttgt ctattaatca gatttgccaa gctgttttta
      781 ttttcgggtt tacaatgggc tataaaacca gcctgatgtt tttttataaa atcaacaaaa
      841 tgtaccggct cattaattac aggcagataa agttgaaaag cttgcttgca agcagctaat
      901 tttattttgt gaatacgttc ggttttgtaa ttcaatcgta ccgaatcgtg gcatattacc
      961 ggtgttattt catgcacacc catttctatt gctttttcaa cgaaccattc aaatctatcg
     1021 gtttgttttg tgggtgcaat agctatatgt aaattatatg gtaatggttg caattggtat
     1081 ttaaatgtta cctgggctat taattgttta tcttgatttt ttataaaaac cgcatcatac
     1141 aaattaccca tgccatcaaa aacttgtgca gtatcaccta tacgcatgcg caaaacttgt
     1201 aatgcatgct tacactcatc atcatctaat gttgccagct tatcaataat attattggaa
     1261 taaaaaaggt gcataaggca aatttaattt ggctacatta agcatgataa ccgatattac
     1321 aaaatggcca ttgttcaaaa tttaatttta gaaagagaaa agttatatag gcattatacg
     1381 aaaaataaaa accaaacgat gatgtaactg ttaccctatt ggtatataat aacaccatga
     1441 ttaattagtt aaccgatgca tgtaagctga tagatactgt ttaacctata aacagggcac
     1501 tgttaataac tatacagttt ttgacccatg caatcatttg attggtaaaa aattcattac
     1561 tacttcgcct ccaaatcatt aaacaaaaaa acaatgaaaa aaacattatt agtattatct
     1621 atggttgcac tgtcatcagt agctatggca caaaaaggca gctggtatgc aggtggcatg
     1681 gttggttttg gatcgtcaac tgacaaagat gctaacgaca aaaaaaatgt aacttcaact
     1741 tggaacttta gccctgaggt tggtacattt ttacgcgatg atatccaatt aggcttagtt
     1801 ttaggcttag gtggtagcaa aacaaaattt gatggtgatg aaacaggcag cacatcaaga
     1861 tttagcccaa ctgtttatgg tcgtaaattt tttaaagtaa ctgagcaatt tagcatgttt
     1921 ggtggcttgt accttagctt aatcaatgaa aaaagtaccg acaaatcatc gggctacgaa
     1981 atcgaaatga aatcaaacgg ttttggtgct aaagttggtg ttggttgcgc ttatgcatta
     2041 tcgccacgtt ttacagcggt aggtcagttt ggtgtattaa atttccaatc ggttagcaac
     2101 gaagttgaag gtaatgatgc aggtaaatca tctgatttcg gtttagatat caacaccttt
     2161 ggtgctgctg catttaatgt aggtttatac tatacattta tgcaatagtg aaaatttaaa
     2221 gaataaaaaa agcctcgaat ttataatgtt cgaggctttt tttatggctt ttatttagtg
     2281 ctgcaattta cttgtcataa aaaattcaat ttcaaataat acaccgtttt tattaataac
     2341 cctaagtgct ccatcaattt gctcaaccaa tccactaacc aattgcatgc ctaacgtttc
     2401 ataattatta atatcaaagc cggcctctaa tcccttgccg ttatcagcta cggttagtac
     2461 tactttacca tctaattctg ctgccgtaac ttctatatat ttattgcgat gttctattgt
     2521 aaaagcatat ttgagcgagt tggttaaaag ctcgtttact ataagtccgc atgcaatggc
     2581 tacatcaata tttaggtgaa tttcctgaac acttaattta agttgaattt gccggccaat
     2641 tgagtaagta aacaataaac tattggcaat actgctgata tattctttaa aattgatact
     2701 cgaaaaatct ttattctgat aaagaagttc atgaatgtag cccatacatc tgatgcgact
     2761 ttggctatcg cgcatggctt cgagggtttg cggatcgttt atgttggctg cctgcaaatt
     2821 aattatactt gaaattattt gcaggttatt ttttacacga tgatgcacct cctttaacag
     2881 cacttccttc tcttgcaagg attgtttaat ggcttcttcg gcttgaagtc tgctggttat
     2941 gtctctaaaa ctccatacac gaccggcata gctgccatta ataagttgtg cttgtgaata
     3001 acgttcaaaa acgcggccat caataaactc aataacatca aaacaaattt catcaggctt
     3061 gtcatacaac cattgtactt ttgccaaaaa atcatccgga tattttaact gatttaccac
     3121 atactgtaat gtgctgttat catcacgcag ttctaaaatc tcaggtggta tgcgccataa
     3181 ttctaaaaac tttttattat acagtgaaac acgccccagc ttatcaacca ataaaatacc
     3241 atcggccgtt gattcgatgg tagcttcgag ctgtgataaa aagtgtgcat tagcttcatc
     3301 gtttttatat tgctcagtta taatctgaga aatgagtatg atgccggtaa ctacctcatt
     3361 attaataaca ggttttacct gaattttaaa ccagctatcg tttccgtttg agccgctgcc
     3421 ataaatattg taagaggcaa ctacctgctc attttttact tgctgatggg ttttgattgc
     3481 caaatcatga aa
//
LOCUS       DAOMAA010000074         3475 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_19978_length_3475_cov_3.494444, whole genome shotgun sequence.
ACCESSION   DAOMAA010000074 DAOMAA010000000
VERSION     DAOMAA010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19978_length_3475_cov_3.494444"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1973
                     /locus_tag="PLO59_01640"
     CDS             <1..1973
                     /locus_tag="PLO59_01640"
                     /inference="COORDINATES: protein motif:HMM:NF019298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HQU99825.1"
                     /translation="LTSYKITAEGIATKGLLGSGNKIIVTQKLVGLQAMLPSHVVQND
                     LIHVPVTISNRANKAIDGQLQIKAPLGFVLQEKLTGAIHIEANSSKTVFVKYHVASWP
                     GTDTLKVQFNAGNISDACKLPVHTLAQGYPVSLAFNGNAMHAKYQALINHAVPGSIKV
                     NVTAFPSVVADLLTGIDGILREPNGCFEQTSMSSYPNILVRNYLKAAGINDAKLEARA
                     NDLIDRGYKKLISFETPQQGYEWFGGAPGHEALTAYGLLQFTDMQAVYAQVDNAMMQR
                     TKQWLLSKRDGNGGYIRNARALDSFGAASKEVTDTYITYALAEAGYNDIDKELKLAYN
                     VAIKSGDAYLLGLAANAHFANKKNKEAETLLEKLITQQDANGSFTGKTHSITRSTHQA
                     LAIETTSLAVMALLRSNNNVMSMQNAVTFLIKSRNGYGNFGNSQSTIMALKALTAYAQ
                     FAKQTNESGQLVCYLNNKLIGKKVYEAGNRNAIVLPNLEKHCANGKAQFEVAIEGAKQ
                     AMPHTLSIQYHTQLPISSDSCMVGLKVALNNHEIRIGETVRLTATINNKTNNGLPSTM
                     VTLGIPSGCQLQMWQLKEMMEKNQLDYFEIKGNMLHIYYRQMKPAEIKTLHFDLLAEV
                     PGIYEAPASSAYLYYTNEFKTWQQMPKLKIKI"
     gene            complement(2029..>3475)
                     /locus_tag="PLO59_01645"
     CDS             complement(2029..>3475)
                     /locus_tag="PLO59_01645"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HQU99826.1"
                     /translation="IDGVPIETPAQVLSVEGVIRNIAPNTPQLSNSISYVFDSWSNGG
                     GATQSITTPVNDTNLVANYNISYRFADNPTNIVNGLYYKFYPGNWTAIPDFNTLTPNK
                     EGIISIFDYSPRTLSDYFGFRFNGYIDVPTDSIYTFYTSSDDGSKLYIGNILVVDNDG
                     VHPMQERSGSIGLKAGKHAITVDYFERNGGQVFSVSWQGPGISKQIIPAASLYRLAGP
                     VNTIAIADAYTRSGSFKNNCLGQTDAARLYSKYAESISDQCRETYLLFDISKFTNEFT
                     SASLDVFGKLKGVDLSSAAEISCLPVANTFWTENTITFANKPTASSNVLAVAYIDDAN
                     GKYYSFDVSNHLKQLLNDGYTQAAFTLKGNFVQNNYVIWNSKESAGNNKPKITCNYLS
                     PRQVSSIIQSNTGIEVFPNPVSGNLITIGVEHYPADIFVFDLQGKLLLKEKIDSDLMQ
                     LPIDKLTNGIYLIQALSKSGIATQKLQVIND"
BASE COUNT         1140 a          654 c          676 g         1005 t
ORIGIN      
        1 cactcacatc ttataagatt actgccgagg gtattgccac taaaggatta ttaggcagtg
       61 gtaataaaat tattgtaacg caaaagttag tgggcttaca agcaatgttg ccatcgcatg
      121 tagttcaaaa tgatctgatt catgtgcctg ttacaattag caacagagcc aacaaagcta
      181 ttgatggcca gttgcaaatt aaagcaccat tgggatttgt tttgcaagaa aagttaaccg
      241 gtgccataca catcgaagca aacagtagta aaaccgtttt tgtaaaatat catgttgcat
      301 cgtggcccgg taccgataca cttaaagtac aatttaatgc cggtaatatt agcgatgcat
      361 gtaagttacc tgtgcataca ttggcacaag gatatccggt ttcattagcc tttaatggca
      421 atgcaatgca tgcaaagtat caagcgctta ttaatcatgc agtacccggt agcattaaag
      481 ttaatgttac tgcttttccc agtgttgtgg ccgatttgtt aaccggtata gatggcattt
      541 tgcgcgaacc caatggttgt ttcgaacaaa catctatgag tagttatcca aatatattgg
      601 tgcgcaatta tttaaaagca gccggaataa atgatgccaa attagaagcg cgtgccaatg
      661 atttaattga caggggctat aaaaaactta taagtttcga aacaccacaa caaggttacg
      721 aatggtttgg cggtgccccc ggccatgaag ctttaactgc ttacggatta ttgcagttta
      781 ccgatatgca agccgtttac gcacaggttg ataatgcaat gatgcaacgt accaaacagt
      841 ggttgctaag taaacgtgat ggaaacgggg gttatatacg caatgcaaga gctttggata
      901 gttttggtgc tgcaagtaaa gaagttacgg atacttatat tacgtatgca ttagccgagg
      961 caggatacaa cgatattgac aaggaattaa aacttgcata taatgtagcc attaaatctg
     1021 gtgatgcata tttattgggt ttagcagcta atgcacattt tgcaaacaaa aaaaacaaag
     1081 aggctgaaac gcttttagaa aaacttatca cacagcagga tgcaaatgga agctttacgg
     1141 gtaaaacgca ttccataacg cgttcaacac atcaggcctt agccattgaa acaacttcgt
     1201 tggcagtaat ggctttatta cgcagtaata acaacgtaat gtcaatgcaa aatgctgtta
     1261 cgtttttaat taagtcgcgc aacggatatg gtaattttgg caattcgcaa agcaccatta
     1321 tggctttaaa agcattaaca gcttatgcac agtttgccaa acaaaccaat gaaagtggcc
     1381 aattggtttg ttatttaaac aataaactta ttggcaaaaa agtgtatgaa gccggtaata
     1441 gaaatgccat tgtactacct aatttagaaa aacattgtgc taacggtaaa gcacaatttg
     1501 aggtggctat cgaaggtgca aaacaagcaa tgccacatac cttatcaatt caatatcata
     1561 cccaattacc cataagcagc gactcatgca tggttggttt gaaagtagcg ttaaacaacc
     1621 atgaaataag gattggtgaa acggttcgat taacggcaac catcaataat aaaaccaaca
     1681 atggcctgcc cagcaccatg gtaactttag gcataccaag cggctgtcag ttgcaaatgt
     1741 ggcagttaaa agaaatgatg gaaaaaaatc agttagatta ctttgaaata aaaggcaaca
     1801 tgctacacat ctattaccgg caaatgaaac ctgcagaaat aaaaacactg cattttgatt
     1861 tgttggccga agtgcccggc atttacgaag cacctgcaag cagcgcttat ttgtattaca
     1921 ccaatgagtt taaaacctgg cagcaaatgc caaaactcaa aattaaaatt taaattatcg
     1981 accgattaat ctacgttaac cgctgtaaga tgtatagtgt tgcagcggtt aatcgtttat
     2041 tacctgaagt ttttgtgtgg caattccgct tttgctcaaa gcctgaataa ggtatatgcc
     2101 attagttagt ttatcaatgg gtagttgcat taaatcacta tctatttttt cttttagcaa
     2161 taattttcct tgtaaatcga aaacaaaaat gtcagcagga taatgttcaa caccaattgt
     2221 tatcagatta cccgaaaccg gattgggaaa tacctcaata ccggtatttg attgtatgat
     2281 tgaacttacc tgacgtggcg ataaataatt gcaggttatt tttggtttgt tgttgccggc
     2341 cgattctttc gaattccaaa taacataatt gttttgtaca aagtttcctt ttaatgtaaa
     2401 cgctgcttgt gtgtaaccat cattaagtaa ttgtttaagg tgattgctaa catcaaacga
     2461 ataatatttt ccattggcat cgtcaatata agcaacggct aaaacatttg aactagctgt
     2521 aggtttgttg gcaaaagtaa ttgtgttctc agtccagaat gtattagcta ccggtaaaca
     2581 tgatatctcg gctgcactcg ataaatcaac accctttaat ttgccaaaaa cgtctaatga
     2641 agccgatgta aactcattgg taaatttcga aatatcgaac agcaaatagg tttcgcgaca
     2701 ctgatcagaa atagattctg catattttga atacaaacgt gctgcatctg tttgacctaa
     2761 acaattattt ttaaacgagc cgcttcgcgt ataagcatca gcaatggcaa ttgtatttac
     2821 gggcccggcc aaacggtata aactggcagc aggaataatt tgcttactaa tacccgggcc
     2881 ttgccagctc acactaaaaa cctgtccgcc atttcgttca aagtaatcta cagttatggc
     2941 atgtttgcca gcctttaatc caatagaccc tgaacgttcc tgcatcggat gaactccgtc
     3001 attatcaacc accaaaatat tgccgatata cagcttacta ccatcatcac tgcttgtata
     3061 aaaagtgtaa atgctatcgg taggcacatc aatataacca ttaaacctga atccaaagta
     3121 atcgcttaaa gtacgaggcg agtaatcaaa tattgaaata ataccctctt tatttggcgt
     3181 caacgtatta aagtcgggta tagcagtcca gttacctgga taaaatttat agtacaagcc
     3241 gttaactata ttggttggat tatcagcaaa acgatacgaa atattgtagt ttgctaccag
     3301 gttggtatcg tttacggggg tagttatact ttgtgttgca ccaccgccat tgctccaact
     3361 atcaaataca taactgattg aatttgaaag ctgaggtgta ttaggcgcaa tgtttcttat
     3421 tacgccttca acacttagta cctgggcagg agtttcgata ggtacaccat caata
//
LOCUS       DAOMAA010000075         3473 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_20005_length_3473_cov_2.713868, whole genome shotgun sequence.
ACCESSION   DAOMAA010000075 DAOMAA010000000
VERSION     DAOMAA010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20005_length_3473_cov_2.713868"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1287)
                     /locus_tag="PLO59_01650"
     CDS             complement(<1..1287)
                     /locus_tag="PLO59_01650"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HQU99827.1"
                     /translation="MILLLFVIYTLLRLGFYVFNADYFDGAGFNFLWFGLRFDAVAIS
                     ITLLPFLVLYLLPFNFFYHKHYQQLLKVIFILINGLAILVNCIDFAYFKFSLKRSGAD
                     VFQIIAMGDDAKNNILSMAYDFWYVPMLSAVLILVVYYRYAKITAPLSKGDGIKGNAA
                     WHLLLIPFIGLAVLGFRGGYQLKPLRIITASDFVQPLHAPLVLNTTFTIIKTFNKEGL
                     EPLNYFHAADAQNIFNPLKNYGDSMFKPLNVVVIILESIGKEYMGCYNAIGYTPFLDS
                     LMKQGLSFTYSFANAKRSVEGLPAVVASIPALMAEPFITSVYDGNTINSLASTLKQQG
                     YGTAFFHGGNDGTMGFDNFASAAGYDMYYGRKAYGTSDFDGNWGVFDEPFYKFFIEKM
                     NGMKPPFHTTFFSLSSHHPYTIPAQYKNKFIKGTLPI"
     gene            1467..3419
                     /gene="thrS"
                     /locus_tag="PLO59_01655"
     CDS             1467..3419
                     /gene="thrS"
                     /locus_tag="PLO59_01655"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018478085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HQU99828.1"
                     /translation="MMNITFPDGNIRTYAAGTTAMAIAKSISEGLARNVLAAKVNGRV
                     QDAQLPINDDAALELLTWDTAEGKATFWHSSAHLMAEAIEFYYPNTKFGIGPAIETGF
                     YYDVDMGDTPFTPDDFKKIEDKMLELAKQANNYQRQAVSKADAIAYFTQKNDPYKLDL
                     IKDLADGSITFYTQGNFTDLCRGPHISDTSPIKAVKLMSIAGAYWRGDEKSKQLTRIY
                     AVTFTKQKELTEYLAVLEEAKKRDHRKLGKELELFAFSEKVGMGLPLWLPKGAALRER
                     LRNFLEKAQMKAGYTPVFTPHIGNKELYITSGHYEKYGADSFKPIKTPAEGEEFFLKP
                     MNCPHHCEIYKSKPRSYKDLPVRLSEFGTVYRYEQSGELHGLTRVRGFTQDDAHLFCR
                     PDQVKEEFCKVIDLVLYVFNALGFTDYKAQISLRDKDDRQKYIGSDENWEKAENAIIE
                     AAAERGLNTFIEYGEAAFYGPKLDFMVKDALKRSWQLGTIQVDYNLPERFQLEYTGSD
                     NQKHRPVMIHRAPFGSLERFIAVLIEHCGGNFPLWLAPDQITILPISEKYNAYGNKVL
                     ETLQNYDIRGLFDDRDEKIGKKIRDAEIKKTPFMLIIGEKEQAENKVSLRKHGAGDLG
                     TFTIDETIAFLQSEIAKDLNQSVKNQ"
BASE COUNT         1185 a          674 c          649 g          965 t
ORIGIN      
        1 tatgggtagc gttcccttga taaatttgtt tttatactga gccggaattg tgtaagggtg
       61 atgtgatgat aatgaaaaaa atgtagtgtg aaaaggcggt ttcattccat tcatcttttc
      121 gataaaaaac ttataaaatg gttcatcaaa cacaccccag ttgccatcaa aatcgctggt
      181 accgtatgcc ttacgcccat aatacatatc atatccggca gcggatgcaa aattatcgaa
      241 acccatagtc ccatcgttac ctccatgaaa aaatgccgtg ccataacctt gttgttttaa
      301 ggtacttgcc aacgagttta ttgtatttcc atcatatacc gaagtaataa acggttcggc
      361 cattaacgca ggtatggatg caaccacggc aggcaaccct tctaccgaac gttttgcatt
      421 tgcaaagcta tatgtaaacg aaagaccttg cttcattaat gaatctaaaa atggcgtata
      481 gcctatggca ttgtaacaac ccatatattc tttaccaata ctttctaaaa taattaccac
      541 tacgtttaat ggtttaaaca ttgaatcacc ataattttta agtggattaa aaatattttg
      601 tgcatctgct gcatggaagt aattaagtgg ttctaatcct tctttgttaa acgtttttat
      661 aatcgtaaaa gttgtattta ataccaatgg cgcatgtaaa ggttgcacaa aatcgctggc
      721 cgtaattatg cgtaaaggtt ttagctgata cccgcctctg aaacctaaaa cagctaaacc
      781 aataaatggt atcagtaaaa gatgccaggc cgcattgcct tttattccat cgccttttga
      841 aagaggtgcg gtaattttcg catacctata atacacaacc aaaattagta cagccgataa
      901 catgggtaca taccaaaaat cgtatgccat actgagtata ttatttttgg catcatcgcc
      961 cattgcaatt atttgaaaaa catcggcacc actgcgtttt aagctaaatt taaaataagc
     1021 aaaatcaata cagttaacaa gtattgccaa gccattaatg agtataaaaa ttactttaag
     1081 taactgctga taatgtttgt ggtaaaaaaa attaaaagga agtaaataca acactaaaaa
     1141 tggcagtagc gtaatagaaa ttgccactgc atcaaaccgc aaaccaaacc ataaaaagtt
     1201 gaatcctgca ccatcaaaat aatcagcatt aaagacatag aagcccaacc taagcaatgt
     1261 ataaattaca aagagcagca atatcaattt tattgcaacc aaatattttt tgaataccaa
     1321 cgacactaca aacaaatatt tacaatgtga ttttaaggcc gcgaaactct aaaaaaagca
     1381 attacttctt ctatatttgc gcgccttttt aagcttttga ttaaaacaaa aatttaaaaa
     1441 cgttaactgt cttacactta aacaatatta tgaacataac atttcctgat ggcaacattc
     1501 gtacgtatgc tgcgggtaca acagcaatgg ctattgccaa atctatttcc gaaggtttag
     1561 cccgcaatgt attagctgca aaagtaaacg gccgggtaca agatgcacaa ttgcctatta
     1621 acgatgatgc agccctcgaa ttattaacct gggatactgc cgaaggcaaa gctacctttt
     1681 ggcattcgtc agcacattta atggccgagg ccattgaatt ttattatcct aacaccaagt
     1741 ttggaattgg ccctgctatc gaaaccggct tttactacga tgtagatatg ggcgacacgc
     1801 cttttacccc ggatgatttt aaaaagattg aggataaaat gcttgagttg gccaaacagg
     1861 ctaataatta ccaacgacaa gcagtaagta aagccgatgc cattgcctat tttacacaaa
     1921 aaaacgaccc ctataaatta gatcttataa aggatttggc cgatggcagc ataacttttt
     1981 atacgcaagg taactttacc gatttatgcc gtggcccgca catttccgat acatcgccca
     2041 taaaagctgt aaagcttatg agtattgcag gcgcttattg gcgtggcgat gaaaaaagta
     2101 aacagcttac acgcatttat gccgtaactt ttaccaaaca aaaagaactt accgaatatt
     2161 tagctgtatt ggaggaagcc aaaaaacgcg atcacagaaa attgggtaaa gagttagaac
     2221 tgtttgcctt tagcgaaaag gtaggtatgg gtttgccctt gtggctacca aaaggagctg
     2281 ctttgcgcga gcgtttgcgc aactttttag aaaaagcaca aatgaaagcc ggctacacgc
     2341 ctgtatttac gccacatatt ggtaataagg aactgtacat tacttcgggc cattatgaaa
     2401 aatatggtgc cgactctttt aaacccatta aaacaccggc cgaaggcgaa gagtttttct
     2461 taaagcccat gaattgcccg catcactgcg aaatttataa atcaaaacca cgcagctata
     2521 aagatttacc cgtacgatta agtgaatttg gcaccgtata tcgttacgaa caaagtggcg
     2581 aattacatgg ccttacacgt gtacgcggtt ttacgcaaga tgatgcacat ttattttgcc
     2641 gccccgatca ggtaaaggag gagttttgta aagtgattga tttagtgctt tatgttttta
     2701 atgctttagg ttttaccgac tacaaagcgc aaatttcatt acgtgataaa gacgaccgac
     2761 aaaaatatat tggtagcgat gaaaattggg aaaaagctga aaatgcaatt atcgaagctg
     2821 ctgccgaacg cggcttaaat acctttatcg aatatggtga agccgcattt tatggtccca
     2881 aattggattt tatggttaaa gatgctttaa aacgaagctg gcaattgggt acgatacaag
     2941 tagattataa cctgcccgaa cgttttcagt tagaatatac cggcagcgac aaccaaaaac
     3001 accgaccggt aatgatacac cgcgcaccct tcggttcatt agaacggttt attgctgtat
     3061 tgattgaaca ttgtggtggg aactttcctt tgtggctggc acctgatcaa ataaccatac
     3121 tacccattag cgaaaagtat aatgcgtatg gaaataaagt tttggaaact ctgcagaatt
     3181 acgatattcg cggacttttt gacgaccgtg atgaaaagat aggtaagaaa atacgcgatg
     3241 ctgaaattaa aaagacccct ttcatgctga ttattggcga gaaagaacag gccgaaaaca
     3301 aagtatcatt acgtaaacat ggcgcaggcg atttaggaac atttaccata gatgaaacca
     3361 ttgcctttct gcaaagtgaa attgccaaag acttaaatca aagcgttaaa aaccaatagt
     3421 attatttaaa acaaaggagg aaatatagca actaataaac ccggaggtta caa
//
LOCUS       DAOMAA010000076         3469 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_20051_length_3469_cov_3.358817, whole genome shotgun sequence.
ACCESSION   DAOMAA010000076 DAOMAA010000000
VERSION     DAOMAA010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20051_length_3469_cov_3.358817"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="PLO59_01660"
     CDS             <1..398
                     /locus_tag="PLO59_01660"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HQU99829.1"
                     /translation="LMKKHCQQQKLNWQIDSAGTIGFHAGEPPDVRSICCCKKHGVDI
                     SMQRASKIELQLLDTYDFIFTMDNQNLADIKNTTAIKLHHLFAILPFAEMTTSHVPDP
                     YYGNTEDFDYVFQLLDEACIRVINKILNK"
     gene            395..1108
                     /locus_tag="PLO59_01665"
     CDS             395..1108
                     /locus_tag="PLO59_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HQU99830.1"
                     /translation="MTAILYLIPCTLGDDDVQKVLTRDALQIINSLDEFIVENEKSAR
                     KFLKATSITKPQSELIIHELDKHQNNTDLLPMLKNCLAGKSIGLISEAGVPAVADPGA
                     LIVAKAHEKNIIVKPLVGPSSILLALMASGMNGQQFCFLGYLPREKDALIQKIKSIEK
                     ESRNKNQTQLFIETPYRNNQLLNEILTVCDAHTYLCLATNITLKSEKIQTKTIKEWRK
                     QKHDIHKQPTVFLIYASNF"
     gene            1187..1786
                     /locus_tag="PLO59_01670"
     CDS             1187..1786
                     /locus_tag="PLO59_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99831.1"
                     /translation="MKNIIYFLLVVLISSCSSQIQFSEPQPPATPNISNFNDDQVGIY
                     LANDSNIIQILPQAVITSYLSANADSTSDVKLDGTLKINNQVHQLNVLSDLKLNFKAD
                     TLATLNQNGVLKKFKNNYYLNVVSDHGYILYCLTFSNNSLILKQQTENDRSILDDLVA
                     EKKTLEDSASIFKPKFSEFKEYIKQKDAFANSKKFYKIN"
     gene            complement(1854..2528)
                     /locus_tag="PLO59_01675"
     CDS             complement(1854..2528)
                     /locus_tag="PLO59_01675"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HQU99832.1"
                     /translation="MQHVAEEGLGCIDYWAQARGHTVTFCKLYEPNYVFPAIESFDWL
                     IILGGPMHVDDTDKHPWLKPEKAFIEQAIKKNKVVFGFCLGAQLIAQILGANVYNMPQ
                     PELGWHSVTCTPLANQHEAFNIFPESFIGFHWHYQMFDLPKHGQLIMSSEACKHQAFI
                     YGKRTIAFQFHPEVTQMGVAEFLANDENKNKSAELKDALVENEMHIAAMHEIMRKFLN
                     KINEML"
     gene            complement(2777..>3469)
                     /locus_tag="PLO59_01680"
     CDS             complement(2777..>3469)
                     /locus_tag="PLO59_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016193394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQU99833.1"
                     /translation="ADKQTDTNIFGWNMPPSMVQNFNSIFVIVLALPFSLFWAWADKM
                     WPNTISPQKQAIGLALIALGYFLIATQVKGLGLDGKIGIIWLVVLYLLHTMGELCLSP
                     IGLSLVAKLSPVRFSSLLMGVWFIANAAGYALAGTLGALLPPTGDKFEKAQKLGIDLK
                     AVLDKSVVPTTEQIAIMKQNNLPEMYPSFVGFQIENLYQFFMLFVIIPLCAAIILFAL
                     SPLMRKLMHGVR"
BASE COUNT         1239 a          631 c          522 g         1077 t
ORIGIN      
        1 gtttaatgaa aaaacactgc caacaacaaa aacttaattg gcaaattgat tcagcaggta
       61 ccatcggttt tcatgcaggc gagccacctg atgtaagaag tatctgttgt tgtaaaaaac
      121 atggtgtaga tataagtatg caacgtgcaa gcaaaattga gttgcaactt ttagatacat
      181 acgactttat ctttactatg gataatcaaa atttagccga cattaaaaac actacagcaa
      241 ttaagttaca tcatttattt gccatcttac cctttgcaga aatgactaca agccacgtac
      301 ccgacccata ttatggtaat actgaagact ttgattatgt gtttcaactt ttagatgaag
      361 cttgtattcg tgtaataaat aaaatattaa ataaatgaca gccatactat atttaatacc
      421 atgcactttg ggagatgatg atgtacaaaa agtattaact cgtgatgcat tacaaattat
      481 taattcgttg gatgagttta ttgtagaaaa tgaaaaaagt gccagaaaat ttttaaaagc
      541 aaccagtatt accaaaccac aatcagaatt gatcatccat gaattagaca aacaccaaaa
      601 caatacggat ttgctgccca tgcttaaaaa ctgtttagca ggtaaatcaa taggcttaat
      661 tagcgaagca ggtgtgcctg cagttgccga tccgggagct ttaattgttg ccaaggcaca
      721 tgaaaaaaat attattgtaa aaccattagt tgggccttcc tcaattttgc ttgcattaat
      781 ggcaagcggt atgaatgggc aacaattttg ttttttagga tacttgccac gtgaaaaaga
      841 tgcactgatt cagaaaataa aatcaatcga aaaagaatcg cgaaacaaaa atcaaacaca
      901 actttttatt gaaactcctt acagaaacaa ccaattatta aatgagattt taactgtgtg
      961 tgatgcacat acatatttat gtttagcaac taacatcaca ctaaaatcag aaaaaataca
     1021 aaccaaaaca attaaagaat ggcgtaaaca aaaacatgat attcataaac aaccaacggt
     1081 gtttttaatt tatgcttcaa atttttagcc ttgttaaatt atggttggta taggttaccg
     1141 ctactaacta ttctgaatta atagtaaact ttaataactg tttactatga aaaacatcat
     1201 ctattttcta ttggtagttc taatatcaag ttgcagttct caaatacaat ttagcgaacc
     1261 tcaacctccg gctactccaa acatttcaaa ttttaatgat gatcaagttg gaatttattt
     1321 ggcaaacgat tcaaacatta ttcaaatttt gccacaggca gttattacat cctatttatc
     1381 agcaaatgca gatagcacat cagatgtaaa gttagatggt acattaaaaa taaacaacca
     1441 agtgcatcag cttaacgttt tgtctgattt gaaattaaat tttaaggccg ataccttggc
     1501 aacacttaat caaaatggtg ttctgaaaaa gtttaaaaac aactactatc tcaatgtagt
     1561 ttccgaccat ggttacatat tatattgtct aacgttttct aacaattcct taatccttaa
     1621 acagcaaaca gaaaacgacc gttccatact tgatgatttg gttgccgaga agaaaacgct
     1681 tgaggattcg gcaagtattt ttaagcccaa attcagtgag tttaaagaat acataaaaca
     1741 aaaagatgcc tttgctaaca gcaaaaagtt ttataagatc aactaatcct aaaccattaa
     1801 aaatttataa gtggcagtaa attttttgtt caatcaaaca cacaacctgg cacttataac
     1861 atttcattta ttttatttaa aaactttcgc attatttcat gcattgctgc aatgtgcatt
     1921 tcattttcaa ccaatgcatc ttttaattca gcggatttat ttttgttttc atcatttgct
     1981 aaaaattcag ctacacccat ttgtgttacc tctggatgaa attgaaatgc aatggtacgc
     2041 ttaccataaa taaatgcctg atgcttacaa gcctcactcg acattatcag ttgtccatgt
     2101 ttaggcaagt caaacatttg atagtgccaa tgaaatccaa taaaagattc aggaaaaata
     2161 ttgaaagctt catgttgatt tgctaatggt gtacatgtta cactgtgcca tcccaattca
     2221 ggttgtggca tattataaac gttagctcct aaaatttgag caataagttg tgcacctaag
     2281 caaaaaccaa aaacaacttt attttttttt attgcttgtt caataaatgc cttttcaggt
     2341 ttaagccacg ggtgtttatc agtatcatca acatgcatgg ggccacctaa aattataagc
     2401 caatcaaaac tttcaatagc aggaaatacg taattaggtt catacaattt acaaaaggta
     2461 actgtatgcc ctctggcttg tgcccaataa tcaatacaac ctaaaccttc ttctgcaaca
     2521 tgctgcaaac aataaatttt aagcttagcc attacttatt atccctgttt tctattttat
     2581 aatctgttgg attggttcaa tagtatcacg ctacttacaa cgtaacattg atttgcgttt
     2641 ttttttgact aacagtaagt acaatcaata tcagtagctt cacaaaaaat ttgatttacg
     2701 aataaacatt acacttcaat atttaaatcg gttcacaaat tttattttct accatgctat
     2761 tcaaatctaa aacctgttat ctaacaccat gcataagttt tcgcattaat ggtgaaagtg
     2821 caaataggat tatagcagca caaagcggaa taataacaaa cagcatgaaa aactgataca
     2881 agttttcaat ctgaaaaccc acaaacgatg gatacatttc gggtaggttg ttttgtttca
     2941 ttatggcaat ttgctcggta gtaggcacaa ctgatttatc aagcacagct ttcaagtcaa
     3001 tgcctaactt ttgtgctttt tcaaacttat caccggttgg tggcaacaat gcacccaaag
     3061 tacctgccaa ggcataacct gcagcatttg caataaacca tacacccata agtaatgatg
     3121 aaaaacgaac aggagataat tttgctacaa gcgataatcc aattggcgat aaacataact
     3181 cgcccatggt atgtaaaaga tataacacta ctaaccaaat gattccaatt ttaccatcta
     3241 aacctaatcc ctttacctga gtagcaatta aaaaataacc caaagctatt aatgcaagac
     3301 caattgcctg tttttgtggc gaaattgtat taggccacat tttatcggcc caggcccaaa
     3361 acaaactaaa aggtaaagcc aacacaataa caaaaatact gttgaagttt tgtaccatac
     3421 ttggtggcat attccatccg aaaatattag tgtcggtttg tttatcggc
//
LOCUS       DAOMAA010000077         3450 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_20250_length_3450_cov_2.438586, whole genome shotgun sequence.
ACCESSION   DAOMAA010000077 DAOMAA010000000
VERSION     DAOMAA010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20250_length_3450_cov_2.438586"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="PLO59_01685"
     CDS             <1..832
                     /locus_tag="PLO59_01685"
                     /inference="COORDINATES: protein motif:HMM:NF006569.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine aminotransferase"
                     /protein_id="HQU99834.1"
                     /translation="AVIRDGDEVIIFDPAYDSYAPAVTLAGGVTIHYDLMAPDYKIDW
                     QAVKKLITQRTRLIIINTPHNPSGTILTVDDLQQLEALISHSNILVLSDEVYEHIIFD
                     GHMHQSVCAFPALAERSFVVYSFGKTYHATGWKLGYVLAPEALTTEFRRIHQFNVFSV
                     NTPMQYALADYMQDAGNYELLPPFYQEKRDKFVSFLQGSRFKIMPCSGSYFQLLDYSN
                     ITDEKDTEYAKQLTIEKGIASIPVSVFYQRKIDNKVLRFCFAKDDETLQRAAEKLLSV
                     "
     gene            845..1660
                     /locus_tag="PLO59_01690"
     CDS             845..1660
                     /locus_tag="PLO59_01690"
                     /inference="COORDINATES: protein motif:HMM:NF007757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HQU99835.1"
                     /translation="MVKAELKVTLVQSKLYWEDATKNLQHFNQLLAGVKAGSTDVIVL
                     PEMFTTGFSMQAHKYAHTTNDFVFEWMFKTAKQKQAVLCGSIMFADNGKYYNRFVWMQ
                     TDGKTQYYDKRHLFSMGTEDKTYTAGQQPLVVMVNGVAIRPLICYDLRFPVYSRNTLN
                     GAGKNLKPAYDVLLFVANWPHVRIDAWRSLLKARAIENACYCIGVNRIGKDANGMAHS
                     GHSKVFNYKGEILHACKPDKTCVDTVTLRINELAVYRSQFAVLNDADNFSLKL"
     gene            complement(1687..>3450)
                     /locus_tag="PLO59_01695"
     CDS             complement(1687..>3450)
                     /locus_tag="PLO59_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99836.1"
                     /translation="LTNDAIGNTITGWNVAGPNTYYNGSSYINNGAAPLPQLIVATQV
                     SRLRPRISFKFRRPYKSFLINIGGDIINNNSYMRSMPHGITPAYNIAGDSGFVAGLST
                     VVFNNAGRNTVNSPGLNNTNAAYYFGIPGQNLATDVDQEITSDNTAATLFNYLEIDKP
                     TLKVIRQNAAVTNIVGGGADSVFLLNGSFELNRRSFTIFNGDSSSLRFANGYFTAEDN
                     VGANAGTMESIVHWQIGNKAGTHIFPFAKNGNRFNFTYSNGTGDDVGLLSVSTYGTPS
                     NNTTYPYPFLPGSNTLHVGSMTTNSLANATPWTVDRFWLIRRSTTAVLSNQATMQFQY
                     PNTEGTAQANYVTGEMKAQRYGFAGGSYNAWESPFAGQSDFSSGGLVTVNVPGMYAHH
                     QNNIWTIVHINAIQNPLPVNLISFTAKLIEKKVHLNWTVAQEQLIAKYQINRTADFNF
                     YENVEQQIAFGNAFTTSYQAVDPMPLPGTSYYQLAIENNDGTINYSNAIAIYNNLKEF
                     SWLQVNANDASVNIAFQYFTGAKLTCTISDVIGNEIYKQTINAIAGKQDAIIPANLSS
                     GIYHICLFDNAQRIATKFIKP"
BASE COUNT         1018 a          640 c          725 g         1067 t
ORIGIN      
        1 agcagtaata cgcgatggcg atgaggtaat aattttcgat ccggcttatg atagttatgc
       61 acctgcagtt acgctggcag gtggtgttac tattcattac gatttaatgg cgcccgatta
      121 taaaattgat tggcaggcag ttaaaaagct gattacacag cgtacacggt taattataat
      181 taatacgcct cataacccat cgggcaccat actcactgtt gatgatttac aacaattaga
      241 ggctttgata agccatagta atatattggt gttaagtgat gaggtttatg agcatataat
      301 ttttgacggc cacatgcatc aaagtgtgtg cgcatttccg gccttagccg aacgcagttt
      361 tgtagtttac tcatttggca aaacctatca tgcaaccggg tggaagctgg ggtatgtttt
      421 agcacctgaa gcattaacta cagagttcag gcgcatacat cagtttaatg ttttttcggt
      481 aaatacacct atgcaatatg ccctggccga ttacatgcaa gatgcaggaa attatgaact
      541 actgccacca ttttatcagg aaaagcgtga taaatttgtt tcatttttac agggcagcag
      601 gtttaaaata atgccctgct caggctctta ctttcagttg ttagattaca gtaatattac
      661 cgatgaaaag gatacggaat acgccaaaca actaactatt gaaaaaggta ttgcttctat
      721 tccggtttcg gttttttatc aacgcaaaat tgataacaaa gtgctgcgtt tttgttttgc
      781 aaaagatgat gaaactttac aacgtgcagc cgaaaaatta ttgtctgttt aaaaagttgc
      841 aattatggta aaagccgagt taaaagttac actggttcaa agtaaattgt attgggaaga
      901 tgcaacaaaa aatttgcaac actttaatca attgcttgct ggagttaaag cgggtagcac
      961 tgatgtaatt gttctgcccg aaatgtttac taccggtttt agtatgcaag cgcacaaata
     1021 tgcacataca acaaatgatt ttgtgtttga gtggatgttt aaaacggcaa aacaaaaaca
     1081 agctgttttg tgtggcagca tcatgtttgc cgataatggt aaatattaca accgctttgt
     1141 gtggatgcaa acagacggca aaacccaata ctatgataaa cgccatttgt ttagcatggg
     1201 taccgaggat aaaacatata cagcagggca acaaccactt gtagtgatgg taaacggtgt
     1261 agctatcagg cctttaattt gctacgattt acggtttccg gtgtacagcc gcaatacatt
     1321 gaatggtgcc ggtaaaaacc tgaagccggc ttatgatgta ttgttatttg tagctaactg
     1381 gccccatgta cgtattgatg cctggcgaag tttgctaaag gcgcgcgcta tcgaaaatgc
     1441 ttgctattgt ataggcgtta accgtattgg caaagatgca aacggtatgg cacacagtgg
     1501 ccacagtaaa gtgtttaatt acaaaggcga aatattgcat gcctgtaagc ctgataaaac
     1561 ctgcgttgat acggttacac ttcgtataaa tgaattggct gtttaccgca gtcagtttgc
     1621 agttttaaac gatgcagata atttttcgct aaagctataa gtataaaaaa tgcatttaaa
     1681 attgcattaa ggttttataa attttgtagc tatccgttgt gcgttgtcaa ataagcaaat
     1741 atgataaata ccactgctaa ggttagccgg tataatagca tcttgtttac ctgcaatggc
     1801 attaatagtt tgcttatata tttcattacc tattacatcg gaaatagtac aagttagttt
     1861 ggcaccggta aaatattgaa aggcaatatt taccgatgca tcgtttgcat taacctgtag
     1921 ccaactaaat tctttaaggt tattataaat ggcaattgca ttgctgtaat taatagtacc
     1981 atcgttattt tcaatagcta actggtaata actggtgccc ggtaatggca ttggatcaac
     2041 agcctgatat gaagttgtaa atgcattgcc aaaagctatt tgttgttcta cgttttcata
     2101 aaaattaaaa tcggcagtac ggtttatctg atactttgct atcaattgct cttgcgctac
     2161 ggtccaatta aggtgtactt tcttttcaat gagtttagcg gtaaacgata ttaaattaac
     2221 cggcaatgga ttttgtatgg cgttaatgtg cacaattgtc caaatattgt tttgatgatg
     2281 ggcatacata ccgggcacat ttacagttac caaaccacct gaactaaaat ccgactgacc
     2341 tgcaaacgga ctttcccaag cattgtaact gccacctgca aagccataac gttgcgcttt
     2401 catttcgcct gttacgtaat ttgcttgtgc tgtgccttcg gtgtttggat attgaaattg
     2461 catggttgcc tgattggata aaacagcagt ggtacttctg cgtattaacc agaaacgatc
     2521 tacagtccag ggtgtggcat tggctaaact attagttgtc atactgccta catgtaacgt
     2581 attgctgccc ggtaaaaaag gataagggta ggtagtattg ttcgatggtg tgccgtatgt
     2641 acttacactt agtaagccca catcatcgcc tgtgccattg ctgtaagtaa agttaaagcg
     2701 attgccattt tttgcgaaag gaaaaatatg tgtgcccgct ttattaccaa tttgccaatg
     2761 cactatactt tccatggttc cggcattggc gcccacatta tcttcggctg taaaataacc
     2821 gttggcaaaa cgcaaactgc tgctgtcgcc attaaaaatg gtaaagcttc tcctgtttaa
     2881 ttcaaaggag ccgtttaata agaaaacaga atcggcaccg ccaccaacta tatttgttac
     2941 tgctgcattt tgacgaataa ctttaagtgt tggcttatct atttctaagt aattaaacag
     3001 ggttgctgcc gtattatcac tggtaatttc ctggtcaaca tcggttgcta aattttggcc
     3061 gggtatgcca aaatagtagg cggcattggt gttatttaag ccgggcgaat tgacagtgtt
     3121 acgtccggca ttattaaaaa caaccgtact taaacctgca acaaagccac tgtcaccggc
     3181 aatattataa gcaggtgtta tgccgtgcgg catactgcgc atatagctgt tgttatttat
     3241 tatatcgcca ccaatgttaa tcaggaatga tttgtatggc cgtctgaatt tgaaactgat
     3301 acgtggccgc aacctactta cctgcgttgc cacaataagt tgcggcaaag gggcagctcc
     3361 attatttata taactgcttc cgttatagta ggtattgggg ccggcaacat tccagccggt
     3421 aattgtattt cctatggcat cgttggttaa
//
LOCUS       DAOMAA010000078         3442 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_20338_length_3442_cov_3.248007, whole genome shotgun sequence.
ACCESSION   DAOMAA010000078 DAOMAA010000000
VERSION     DAOMAA010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20338_length_3442_cov_3.248007"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="PLO59_01700"
     CDS             complement(<1..417)
                     /locus_tag="PLO59_01700"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HQU99837.1"
                     /translation="MKKIKIITALLLIISSTYSQNWQWAKHAGGSGNDRVSSICNDVN
                     GNLYIGGGANGTNCIFDTISLNCFYPTMILAKYNPNGNIVWLKKANGSNTGAYAKKIG
                     IDGNGFIYVAGFFQGNGTFDTTTLTGYGNDDGFLAKY"
     gene            complement(430..1257)
                     /locus_tag="PLO59_01705"
     CDS             complement(430..1257)
                     /locus_tag="PLO59_01705"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU99838.1"
                     /translation="MQGSTTYYPANGHPPFYSITTFTDANGNFNLLPYTNTSELTYKA
                     ISDVRVTCAGCEVKYFNWTDPFTMVLYLPPSIELYKNTTKFDNHINHLVYIQNNTTQN
                     FSANNTILVDNNFTVEGTGLTAGGTADVRAKNEVTIKGEFHAQYGSETIIHTGNPLPD
                     CSIVTFRNASTSGVATNQKDVTKNIELAFNTQTQFSFDVLPNPNNGNFDIIISPLSKS
                     TAAFYTGKLYNLVGNCVVTIAPFNDKLNLNLNHLAKGFYYLTLINNNKQVTQKLIIQ"
     gene            complement(1434..2621)
                     /locus_tag="PLO59_01710"
     CDS             complement(1434..2621)
                     /locus_tag="PLO59_01710"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU99839.1"
                     /translation="MYTTANCQLQITPILLDSGLYVHDIDVLSQDTMLFTSTSKIYRT
                     TNGGASWDSIETNLPGSVSVDFLNFNIGVAVNYNSIKRTTNAGVTWDSLAFASTTSNR
                     DCHIIDSSHFIVSNDSGYIRHVYTNYYVDDTLLDPLALGSPVVIFDFDFLDDSVGFGG
                     GVVNGFSAVIHTADNGLTWDVRANSGFLYLYLHEISFVDSMVGWATQDSPGLWEHISF
                     TTDGGYTWQSGYNYFDEKRMNGLDFSPSGLGLVLTKSGYIFQTINSGQSWDTAYAYQS
                     GGPYGVKVKIVNDSVAYLGGARGIYKLTNLPTGIFENNINKIKIYPNPATSILNIQIP
                     SNQKANKVEMYDMQGRLVLLSNAVNHNPLPLNVAGLTPGIYAVKVICNASTHYTKVVI
                     EAP"
     gene            complement(2657..>3442)
                     /locus_tag="PLO59_01715"
     CDS             complement(2657..>3442)
                     /locus_tag="PLO59_01715"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU99840.1"
                     /translation="NSVNFINNNSFDTYNSISIDDGATWQHYNISDNSTPSIQLPNYI
                     AHDYNGNDLCNGLGIPAWCDGRNGFQTVYTQPYEIPCATNLDLCNPVTPYYKIEKAQN
                     NIGVAQNCAYNIPSTTDIRMFAGNSIHLNSGFHASAGSKFHAVINANCVPENVKRISN
                     NGLINKQSQFKNSRTILSVNPNPVIDELNISLTNLNTETVSIHIHAIDSKKTEKVLEN
                     FSIKNLSSANFSFSVKTLNLKAGVYLIETRFNSEVITAKILIQ"
BASE COUNT         1100 a          617 c          584 g         1141 t
ORIGIN      
        1 atatttggct aaaaacccat catcgttacc atagccggta agggttgttg tatcaaatgt
       61 accattaccc tgaaagaaac ctgcaacgta tataaagcca tttccgtcaa tcccaatttt
      121 ttttgcgtat gccccagtgt tcgaaccatt tgctttttta agccacacta tattaccatt
      181 gggattatac tttgcaagta tcattgtagg gtaaaagcaa tttaaactaa tcgtatcaaa
      241 aatgcaatta gtaccatttg caccaccacc tatatatagg tttccgttta catcattaca
      301 aatggaactt actcggtcat ttcctgaacc tcctgcatgt ttagcccatt gccagttttg
      361 agaataagta ctgcttataa ttaatagtag tgctgtaatt atttttatct ttttcatagc
      421 ttttactttt tattgaatga tgagtttttg tgttacttgt ttattgttat tgattaacgt
      481 gaggtaataa aaacctttgg ctaaatgatt taggttaaga tttaatttgt cattaaaggg
      541 tgctattgtt actacacaat tgcctactaa attataaagc ttaccggtat aaaatgcggc
      601 tgttgatttt gacaatggtg aaatgatgat atcaaaatta ccattattag gattgggcaa
      661 cacatcaaaa ctaaattgcg tctgtgtgtt aaaagctaac tcaatatttt ttgttacatc
      721 tttttgattt gttgctacac ctgatgtgct ggcattgcga aaagttacta tactacaatc
      781 gggcaaagga ttaccggtat gaataatagt ttcgcttcca tattgtgcat gaaactcccc
      841 ctttatggtt acttcgtttt tagctcttac atcggcagtg ccacctgccg ttaaaccagt
      901 accttcgact gtaaaattgt tgtcaaccaa aatggtattg ttagccgaaa agttttgtgt
      961 ggtattgttt tgaatgtaaa ccaagtggtt tatgtgatta tcaaacttag tggtgttttt
     1021 atagagttca atactaggtg gcaaataaag taccatagtg aatgggtctg tccaattgaa
     1081 gtactttact tcacaaccag cacaggtaac ccttacatca gaaatggctt tatatgttaa
     1141 ttcacttgta ttagtataag gtaataagtt aaagtttcca tttgcatccg taaaggtggt
     1201 tattgaataa aatgggggat gtccattagc aggatagtaa gtagtcgatc cttgcacgat
     1261 agcatcttca ataggaaagc cgttttggtc aactacttgc gaggtatata cagtaccggc
     1321 tccatgatta aaattaaagt aatttgatgt tgaagccgcg aattccctta aacaccaaat
     1381 ctctgattaa gtgaaattgc tgcctacaac ccaaattcac caacttatta aagctaaggt
     1441 gcttcgataa ccaccttcgt ataatgggta cttgcattac aaataacctt tacagcgtat
     1501 atgcctggtg ttaggcctgc aacatttaac ggcaagggat tatgattaac agcattgctt
     1561 agtaaaacca atcggccttg catatcatac atttccactt tattagcttt ttggtttgat
     1621 ggaatctgta tgtttaaaat tgaagttgct gggtttgggt aaatctttat tttattaata
     1681 ttattttcaa atattccagt gggaagattg gtgagtttgt agatgccacg tgcaccacca
     1741 aggtaagcaa cggagtcatt tacaatcttt acctttacac cataaggtcc gcctgattga
     1801 taggcataag cagtatccca cgattgaccg ctattaatag tttgaaaaat gtatccactt
     1861 ttagttaata ccaaacccaa accggaaggg ctaaagtcaa gaccgttcat acgtttctca
     1921 tcaaaataat tatatcccga ttgccatgta tagcccccat cggttgtaaa tgaaatatgt
     1981 tcccacaagc cgggactatc ttgtgtagcc caccccacca tactgtcaac aaaagatatt
     2041 tcgtgcaagt acaaatacaa aaagccactg ttagccctta catcccatgt taggccatta
     2101 tcagcagtat gaataacggc cgaaaaacca ttaactactc ctcctccaaa tccaacactg
     2161 tcatctaaaa aatcaaaatc aaaaatcaca acgggagacc caagtgccaa tggatctaac
     2221 aaggtatcgt caacataata attagtataa acgtgccgta tgtaaccact atcattagaa
     2281 actataaaat gactgctatc tataatatgg caatcacgat tactagttgt tgatgcaaaa
     2341 gcaagcgagt cccatgttac acctgcattt gtagtacgct ttatcgaatt ataatttacg
     2401 gctactccaa tattaaaatt caaaaagtca acagaaacac tgcctggcaa atttgtttca
     2461 atactatccc aacttgcccc tccgttcgtt gtacgataaa tttttgaagt tgaagtaaac
     2521 aacatggtat cttgacttaa aacatcaata tcatgtacat acaaacctga gtcaagaagt
     2581 atgggcgtaa tttgaagttg acagtttgca gttgtgtaca cgcaaaataa tagcttaaaa
     2641 agcaaagtgt attttctcat tggattaaaa ttttagcagt aataacttct gaattaaatc
     2701 ttgtttcaat taaataaaca ccagctttca ggtttaatgt tttaactgaa aaactaaagt
     2761 tagcggaaga aagattcttg attgaaaaat tttctaaaac tttttctgtt tttttagagt
     2821 ctattgcatg gatatgaatc gaaacagttt ccgtgtttaa attagttagt gaaatattta
     2881 attcatctat gacaggattt gggttaacgg ataaaatagt tcttgaattt ttaaattgac
     2941 tttgcttatt aatcaaacca ttatttgaaa ttcttttcac attttccggt acgcaattcg
     3001 catttattac tgcatgaaat ttactacctg ccgaagcatg aaagccacta tttagatgta
     3061 ttgaattgcc tgcaaacatt ctgatatctg tagtggacgg aatgttataa gcacaatttt
     3121 gtgctacacc aatattgttt tgtgcctttt ctattttata atagggcgta accggattgc
     3181 ataaatccaa attggtcgca caaggaattt cataaggttg tgtatatacg gtttgaaatc
     3241 cattccggcc atcacaccaa gctggaatac ctaaaccatt acacaagtca tttccattat
     3301 aatcatgagc aatataattg ggtaattgaa ttgatggagt tgaattgtca ctaatattat
     3361 agtgttgcca ggttgcacca tcatctattg aaatcgaatt gtaagtatcg aaggaattat
     3421 tattaataaa attaactgaa tt
//
LOCUS       DAOMAA010000079         3442 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_20341_length_3442_cov_3.126366, whole genome shotgun sequence.
ACCESSION   DAOMAA010000079 DAOMAA010000000
VERSION     DAOMAA010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20341_length_3442_cov_3.126366"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /locus_tag="PLO59_01720"
     CDS             complement(<1..972)
                     /locus_tag="PLO59_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99841.1"
                     /translation="MSQKLNTQVSIQHVNIKFFNAVELQQVFIADQQNDTLLFANSVT
                     AKISLLKFNQQKVTISDINLNNGRIGLKRFAGSGDYNFDFIINALQSNDTTSTPSAWD
                     IKLEKIALINQTFSYKDYRYNDTTPCINFEDIYLTNLNVIANDIVLYKDSVALKINQI
                     SLHEKSGFVVNNLQADAVIADTLIRLRNLSITTPNSKIDGHYRMEFTSYNDFNDYIDK
                     VFMRAEFKPSTVSSNDIQYFAKELFGLNTTLKISGVARGTVNHLKLKNLDVSCHNVTR
                     FKGSATMDGLPNVEQTFFNVLVDEFVSNKEDIEKIPEFPFNKNQKIRL"
     gene            1214..2230
                     /gene="tsaD"
                     /locus_tag="PLO59_01725"
     CDS             1214..2230
                     /gene="tsaD"
                     /locus_tag="PLO59_01725"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009185219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HQU99842.1"
                     /translation="MKNEVLILAIESSCDETSCAVSINDKILANVTATQAIHASYGGV
                     VPELASRAHQIHILPVVHEALKQAGINKNAIHAVAFTQGPGLLGSLLVGGSFAKSFAQ
                     ALQLPLLAVNHMQAHVLAHFIRHSNNQQMPQFPFLCLTVSGGHTQIVLVKDYLQMEVI
                     GQTLDDAAGEAFDKAAKFLGLPYPGGTLVDKYASEGIAKYNFALPQVADLNFSFSGLK
                     TGFLNFINKKLQAQPDFIELERNHICASIQQAIITMLMQKLSQATLQYNINQIAIAGG
                     VSANKGLRKTLQDHTQKGWHVYIPPFEYCTDNAAMIAIAGYYQYLQHKFVGYDAVSMP
                     RFAF"
     gene            complement(2227..2958)
                     /locus_tag="PLO59_01730"
     CDS             complement(2227..2958)
                     /locus_tag="PLO59_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HQU99843.1"
                     /translation="MKKDSTSWFSPALNKEMPIVKYGHSGFVLLLVPTAAADYLEYER
                     FQLIDALAPLIDSGKVTVYSIDSINNESWLNNKMEGAHKAIRHNQFNQYVFNEVIPFI
                     KTNSGEDTPIITCGASFGALHSMNLFLKRPDLINGVIAMSGVYNLSDYTKGYYDEQVY
                     FNSPMHYLPNLNDHTILEQIRKSNHIFVLTGSGAYEDPEASKAFAGVLYNKGITYKLD
                     VWDGQWKHDWPTWRNMLPFYIANFF"
     gene            3153..>3442
                     /locus_tag="PLO59_01735"
     CDS             3153..>3442
                     /locus_tag="PLO59_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99844.1"
                     /translation="MEEQNQKSSKLSTGILAVCLVASLGLNFYQFNKGQNTMATYETK
                     VDTLEMARTEVEAELNATYAELEKFKGQNAQMDSILNEANVKMDEQKSRING"
BASE COUNT         1111 a          559 c          628 g         1144 t
ORIGIN      
        1 cagcctaatt ttttgatttt tgttgaaagg aaattccgga attttttcaa tgtcttcttt
       61 gttcgacaca aattcatcta ccaatacatt aaaaaaagtt tgctcaacat ttggtaaacc
      121 atccatggtt gccgagcctt taaaccttgt aacattgtgg caggatacat ctaaattttt
      181 taattttaaa tgattaaccg ttccgcgcgc tacaccacta atttttaatg tagtattaag
      241 gccgaatagt tctttggcaa agtattgtat atcattagat gatacggtgc tgggtttaaa
      301 ttcagcacgc ataaatacct tgtctatgta atcattaaaa tcattataag aagtaaattc
      361 catccggtaa tggccatcaa ttttactgtt aggggttgta atgctcaggt ttcgtaaacg
      421 tatcaaagta tcggcaatta ctgcatcggc ttgcaggttg tttactacaa atccgctttt
      481 ctcatgcaat gaaatttgat taatttttaa tgcaacagaa tctttatata atacaatatc
      541 gttggcaata acatttaggt ttgttaggta gatgtcttca aaatttatac agggcgttgt
      601 atcgttgtag cgataatctt tataactaaa tgtttgattt atgagggcaa ttttttcgag
      661 tttaatgtcc catgcacttg gtgttgaagt tgtatcgtta gattgtagtg catttataat
      721 aaaatcgaaa ttgtaatcac cactgcctgc aaatcgttta agcccaatac gtccattgtt
      781 taagttaata tcgcttatgg taactttttg ttgattaaac tttagcagcg atattttagc
      841 tgtaacactg tttgcaaata ataaagtgtc gttttgttga tctgcaataa aaacctgttg
      901 caattcaaca gcgttaaaaa atttaatatt tacatgttga atgcttactt gtgtatttag
      961 tttttgtgac aactgctttg tagcaaattg aaccaatttt gtttgaatgt ccggaatttg
     1021 aattgaaata ccaattgcaa ccatcaataa caaaatggtt gtgaaaatat aaagcaatat
     1081 tttaatggta cgcttaataa ttaaagttga ttaatggttt ttcttttcat ttttgcccta
     1141 atgtaaccgc atagtttgca aagttaactt tatagataat agccaaacga ttagcgaaac
     1201 aataaaagtg tgtatgaaaa atgaagtact gattttagct atagaatcat catgtgatga
     1261 gacttcatgt gccgtatcaa taaacgacaa aattcttgcc aatgttacgg caacacaggc
     1321 tatacacgca agttatggag gtgtagtgcc cgagcttgca tcaagggcac atcaaataca
     1381 tattttgcct gttgtgcatg aagctttaaa gcaagccggt attaataaaa atgcaataca
     1441 tgctgttgct tttacgcaag ggccaggttt gttaggttct ttgttagtgg gtggcagttt
     1501 tgcaaaatcg tttgcacaag ctttgcaatt gccattactt gctgttaatc atatgcaagc
     1561 gcatgtgcta gcacatttta ttcggcatag caataaccaa caaatgccgc agtttccgtt
     1621 tttatgttta accgtatcgg gtggccacac gcaaatagta cttgtaaagg attatttgca
     1681 aatggaagta ataggacaaa cattagatga tgcagccggt gaggctttcg acaaagcagc
     1741 aaaatttttg ggcttacctt acccgggtgg gactttggtt gataaatatg ctagcgaagg
     1801 cattgctaaa tataattttg cattaccaca ggttgccgat ttaaatttta gttttagtgg
     1861 gttaaaaaca ggtttcttaa attttattaa taaaaaattg caggcccaac ccgattttat
     1921 agagctggag cgcaatcata tttgtgcaag catacaacaa gctataataa ccatgcttat
     1981 gcaaaaattg tcgcaggcaa cattacagta taacattaac caaattgcaa ttgcaggagg
     2041 tgtgtcggct aacaaaggtt tgcgaaaaac gttacaagac catacgcaaa aagggtggca
     2101 tgtttatata ccacccttcg aatattgtac cgataatgca gcaatgattg ctattgcggg
     2161 ttattatcaa tacctgcaac ataaatttgt tggttatgat gctgtatcaa tgccgcgctt
     2221 tgcattttaa aagaaattag caatataaaa aggtaacatg ttgcgccatg taggccaatc
     2281 gtgcttccat tgaccatccc atacatctaa tttataagta atacctttat tataaagcac
     2341 accggcaaaa gcttttgaag cttcggggtc ttcgtaagcg ccacttccgg ttaatacaaa
     2401 tatgtggttg ctttttctga tttgctccaa tattgtatgg tcgttaagat tgggtaaata
     2461 atgcataggc gagttaaaat aaacctgctc atcataatag ccctttgtat aatcagataa
     2521 gttatatacg ccactcattg caattacgcc attaattaaa tcaggtcgtt ttaagaaaag
     2581 gttcatgcta tgtaatgcac caaatgaagc accgcaggtt atgattggtg tatcttcgcc
     2641 actgtttgtt ttaataaatg gaataacttc gttaaacacg tattggttaa actgattgtg
     2701 ccttatggct ttatgtgcac cttccatttt attgttaagc caactttcgt tgtttatact
     2761 atcaattgaa taaacggtaa ccttaccgct gtcaattaat ggcgccaaag catcaattag
     2821 ttgaaagcgt tcatactcca gataatcggc tgctgctgtt ggtactaata ataaaacaaa
     2881 accactatgg ccgtatttta caattggcat ttctttgttt agtgctggac tgaaccaact
     2941 ggtgctgtct tttttcataa tattttagat gtaagctttg agccttcgaa ttaatcattt
     3001 atgttttcaa taagcaatat tttaattata gtttctttga aatagactaa ataaataaag
     3061 tgaaagggaa gaaattattc ataaaaaatt aattcggcac gatttttaca tttgtagcct
     3121 taattagaaa tcaaatttaa atacatataa aagtggaaga acaaaatcaa aaatcatcaa
     3181 aattaagcac aggcattcta gcggtttgtt tggttgcatc attaggcctt aatttttacc
     3241 aatttaataa gggtcaaaat acaatggcta cctacgaaac taaggttgat acattagaaa
     3301 tggctcgtac agaagtagaa gctgaattaa atgcaaccta tgccgagtta gaaaaattta
     3361 aagggcaaaa tgcacaaatg gatagcattt taaatgaggc aaatgtaaaa atggatgagc
     3421 aaaagagccg cattaatggt tt
//
LOCUS       DAOMAA010000080         3412 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_20691_length_3412_cov_3.200775, whole genome shotgun sequence.
ACCESSION   DAOMAA010000080 DAOMAA010000000
VERSION     DAOMAA010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20691_length_3412_cov_3.200775"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1772)
                     /locus_tag="PLO59_01740"
     CDS             complement(<1..1772)
                     /locus_tag="PLO59_01740"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HQU99845.1"
                     /translation="MKKQFFVIMLPFLIGSACAQTLTPEILMSLKRVNEPRLSPDGSK
                     ILYQVRTMDVGANTGNTDIYIMDADGKNVKPLANTATNETSAKWIEQGNKIAYLTETG
                     GETQLFEMLPDGGNKTQVTAHKGGITNYGYSKQGNYFWFSADVKLEQNPAEIYPDLPK
                     VNGARIIDGLNYRHWNVWSDYTYSHIFVGRKVGGKLISTNDIMSNERFDAPNKPNDGD
                     EQMAFSADEKYLAYACKKLRGTDYAVSTNTDIYLFDLTANTTTNISSNMPGYDNMPVF
                     APDNKSILWLSMAEAGYEADRTRLMQCDLATKNTKELLSNFDYSIGKFDFNALGNKIY
                     FIAGVEATDQLFSLDVASKSKNTLRKITNVEADITDLSIATKNKEDKIIVALMRHDLP
                     TEIFNVDLKTGNTLQISAVNKALLSNIKMGKSQKRMVKTSDGKAMLTWVIYPPDFDPA
                     KKYPALLYCQGGPQSTVSQFFSYRWNFELMAANGYIVVAPNRRGLPSFGETWNDAIRN
                     DWGGQAMTDLLSAIDDVANEPYVDKNKLACVGASFGGYSAFWLAGHHNKRFKAFIAHC
                     GVFNLESMYGTTEEIWFTNTDMGGP"
     gene            complement(1786..3336)
                     /locus_tag="PLO59_01745"
     CDS             complement(1786..3336)
                     /locus_tag="PLO59_01745"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU99846.1"
                     /translation="MINKKLAMLLTSMLTYTGSMAQSVFQKTYTQGINTEFTQIINTS
                     DGGFANIGSFNNSNDDICLVKTDAIGNVQFTTTLTSPTLENAKGVIQTNSGDYIICGN
                     ITINNTNAIYLAAINASGSLLWQKYYDAGKTDVANSIKLAANGDFIIAGYTTSVGIAY
                     PKGLLMRTDAAGNLLWSKAYTQIHTQTFNKVIEASDGSLIAVGSQVAFGNTDYDWVVL
                     HTDSAGTLIWNYTYGDLSDEEAYDIIQVGGYYYVTGYTASAGAGSRDMFVMQLGLAGN
                     ILGGTVIGTEKSETAFNIHYYDNTSLAITGVSEITSAAGNKEVGTILRTDFLLNPYQA
                     TYLGDSNAVFNPISSVVNATGKIALSGKYKAPGNTNFEGLLMQNNLAYSAACNELYPT
                     ILTSNYIPNHNAQTTELLVTFATQAANLVANNPTITLNTLCFSTGLENKNKHLVGYPN
                     PATDFINLDAIWHGSSIQISNLQGKLSIEATLSNDYKIDVKSLQPGLYIATINKYQEQ
                     FRFKFVKQ"
BASE COUNT          960 a          660 c          653 g         1139 t
ORIGIN      
        1 gggccaccca tgtcggtgtt tgtaaaccaa atttcttcag tggtgccata cattgattct
       61 aaattaaata caccgcaatg ggcaataaaa gctttaaacc ttttgttgtg atgaccagct
      121 aaccaaaatg cagaataacc accaaagctt gcacccacac aagccaactt gtttttatca
      181 acgtatggtt cattggccac atcatcaata gccgaaagca aatcggtcat ggcttgtccg
      241 ccccagtcgt ttctaatggc atcattccag gtttcgccaa aagatggcaa tcccctgcgg
      301 ttaggtgcca ctacaatata accattagcg gccataagtt caaaattcca tcggtaacta
      361 aaaaattgcg aaacggtact ttgcggcccg ccctggcaat aaagtaatgc agggtatttt
      421 tttgccgggt cgaaatcggg tgggtatatt acccacgtta gcatggcttt gccatcgctg
      481 gttttaacca tacgtttttg cgacttgccc atttttatgt tgcttaacag tgctttgttt
      541 acagcagaga tttgtaatgt gtttccggtt tttaaatcaa cattaaaaat ttcagtaggt
      601 aaatcgtggc gcataagggc aacaattatt ttatcttctt tatttttggt ggcaatgctc
      661 aagtcggtaa tatcggcttc tacattggta attttgcgta aggtgttttt gctttttgat
      721 gcaacatcta aactaaaaag ctggtcggtt gcttctacgc ctgctatgaa atatattttg
      781 ttgcccaaag cattaaaatc gaacttgcca atactgtaat caaagttgga aagcaactct
      841 ttggtgtttt ttgtagctaa atcgcattgc attaaacgag tacggtcggc ttcgtaacca
      901 gcctctgcca tgcttaacca aagtatggat ttgttatcgg gagcaaaaac aggcatgttg
      961 tcatatcccg gcatattgct gctaatattg gttgtggtat tggcagttaa atcgaataaa
     1021 taaatatcgg tgttggtgct aactgcgtaa tcggttccgc gtaatttttt acaggcataa
     1081 gccaggtatt tttcgtctgc actaaatgcc atttgctcat caccatcgtt gggcttattg
     1141 ggtgcatcaa aacgctcatt gctcataatg tcgttggtgc ttatcagttt cccacccact
     1201 ttacgtccta caaaaatgtg cgagtaggta taatcactcc aaacattcca atgacggtag
     1261 tttaatccat caataatgcg cgcaccgtta actttaggta agtcaggata aatttcggca
     1321 gggttttgtt ccaatttaac atcggcacta aaccaaaaat aattgccttg tttcgagtat
     1381 ccgtaattgg ttatgccgcc tttatgggcc gtaacctgtg ttttattgcc accatcgggt
     1441 aacatctcga aaagttgtgt ttcgcctccg gtttcggtta ggtaagcaat tttattgcct
     1501 tgttcaatcc attttgccga ggtttcattt gtagcggtgt ttgccaatgg ttttacattt
     1561 tttccatcgg catccataat ataaatatcg gtattgcccg tatttgcacc tacatccata
     1621 gtacgaacct ggtataaaat tttgctgcca tcgggcgata aacgtggttc gttaacccgt
     1681 ttaagcgaca ttaaaatttc aggggttaaa gtttgcgcac atgcacttcc tattaggaaa
     1741 ggcaacatta ttacaaagaa ttgttttttc atgcgggttg catttttatt gttttacaaa
     1801 tttgaatcga aattgctctt ggtatttatt tattgttgct atataaagac cgggctgtag
     1861 cgattttaca tcaattttat aatcgtttga taaagttgct tctatactaa gctttccttg
     1921 caaattactg atttgaattg aagatccatg ccaaattgca tctaaattta taaaatcagt
     1981 tgccggattt ggatagccaa ccaaatgctt gtttttattt tctaatcctg tactgaaaca
     2041 caacgtattg agtgtgatgg ttggattgtt ggcaactaaa tttgcagcct gtgtggcaaa
     2101 agttaccaat aattcagtgg tttgtgcatt atggtttgga atatagtttg aagttaaaat
     2161 tgtaggatat agttcgttac atgctgcact gtaagccaaa ttgttttgca tcagtaaccc
     2221 ttcgaagttg gtattgccgg gtgctttgta ttttccactt aatgctattt tacctgttgc
     2281 gttaacaaca gaagatatgg gattaaaaac cgcatttgaa tcacccaaat aggtagcctg
     2341 atagggattt aataagaagt cagtacgaag tatggttcca acttctttat taccggctgc
     2401 actggtaatt tccgaaactc cggttatagc caacgatgta ttatcgtaat agtggatatt
     2461 aaaagcagtt tcgctttttt cagtaccaat taccgtaccg cctaaaatat tacctgccaa
     2521 acctaattgc atcacaaaca tatcgcgact accggcacct gccgatgcag tataaccggt
     2581 aacataataa tatccaccaa cctgtattat atcataagct tcttcatcgc ttaaatcacc
     2641 ataggtatag ttccaaatta atgtgcctgc actatcggta tgcaaaacaa cccaatcgta
     2701 gtcggtattt ccgaaagcaa cttggctacc cacagcaata agacttccgt cacttgcttc
     2761 gataacttta ttaaatgttt gagtgtgtat ttgtgtgtaa gctttcgacc ataaaaggtt
     2821 tccggcagca tcggtacgca ttaataaacc tttaggataa gcaataccaa ctgaggtagt
     2881 ataacctgca attataaaat cgccatttgc tgccaatttt atactgtttg caacatctgt
     2941 tttgccggca tcataatatt tttgccataa taaactaccg gaagcattaa tggctgccag
     3001 gtaaatagca ttcgtattgt taatggtaat gttaccacaa attatataat ctccgctgtt
     3061 tgtttgaata acgccttttg cgttttctaa ggttgggctt gttaaagtgg tagtaaactg
     3121 tacattgcca atagcgtcag ttttaactaa gcaaatatca tcattggagt tattaaatga
     3181 accaatattt gcaaagcctc catcagatgt attgataatc tgtgtgaatt cggtgttaat
     3241 accttgcgtg taagtttttt gaaatacaga ttgcgccatt gaacctgtat aggtaagcat
     3301 gctggttagc agcatggcaa gttttttatt aatcattatt ggccttgttt actttctttt
     3361 taaagggctg caaaagtaag cattgtatct taattttcaa atcttaaatt aa
//
LOCUS       DAOMAA010000081         3401 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_20798_length_3401_cov_2.962343, whole genome shotgun sequence.
ACCESSION   DAOMAA010000081 DAOMAA010000000
VERSION     DAOMAA010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20798_length_3401_cov_2.962343"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..1221)
                     /locus_tag="PLO59_01750"
     CDS             complement(100..1221)
                     /locus_tag="PLO59_01750"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HQU99847.1"
                     /translation="MKTNFTFAPCLSGINLIMQTSGFHIKGFDGLRALSILLVLFTHL
                     GWYDALPDNYFFNALEQSITGMVGVTMFFTISGFLITTLLLREKAKNGTINYKHFIVR
                     RFLRLLPVLVLFYFTMCILILTHQLNISWVAFFISVFYGYNFVPNRLYFSELGHTWSL
                     AVEEQFYILWPWVVLFFKKNGLLLVALFMLALAILALIVLPTYRITFNGKSYFIGESF
                     HANRFFLPAIAPIMIGSIAAALHFFYPEKLIAFCSSMVNKLLIVITMLAAFWLPDSLQ
                     TALFLIHALCFAWVLGYIHSKQQSLITTILEWRPLAFTGKISYGIYVWQGLFLGTGPG
                     GKLWIQQPIQNLLLTWLVAIACYYLVEVKVLRLKKHFIA"
     gene            1312..1503
                     /locus_tag="PLO59_01755"
     CDS             1312..1503
                     /locus_tag="PLO59_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013928710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HQU99848.1"
                     /translation="MVNSIFLGIMGMGGSELMIILVLVLVIFGGSKIPSLMKGLGQGI
                     REFKDASERKDDKKDITKT"
     gene            1692..>3401
                     /locus_tag="PLO59_01760"
     CDS             1692..>3401
                     /locus_tag="PLO59_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1736 domain-containing protein"
                     /protein_id="HQU99849.1"
                     /translation="MKKNIAPKKTTPSSITTPQTAAAFKRLMLIVCAVFAFLLYANTL
                     THDYTVDDDTVINNNKITKKGVSALPEIFTTAYRAGFWERKESLYRPLSVAMFAVEYS
                     IAPGNATVGHIVNVLLYAATACVLLLVLLALLKDYNQLLPFAITLLYIAHPLHTEVVA
                     NIKSRDELLCFFFSILSIWHMLAYLDKQKTSSLVYCSFSFFLALISKENALTFVAVAP
                     LSVYFFTKATGKQVWQIAGIYLMIAVAYLALRIAILGGATNFTEIQLINNSLIGAGDD
                     WGKRLASAIYIMGRYLWLLLFPHPLSFDYSYNTFPLVSFTDFRALLVLVIIGLLLYYV
                     IKNFTKKSPIAFAILFFAATISLVSNVVFLIEATLGERFAYMPSLGYCMALPFVLAII
                     FKVQLQRQSYQSLQHMFAKNKNMALVLGVILCLYSVKTVSRNFDWKDNFTLLTTDVKT
                     NTESARIRYALGSEYVLARALKADSLNEAQKAIDLDSGIVHLERGVQILPSYSDAWYH
                     LALAYKEKSDQTNAIRCFEQARSMKQFKEIDFYVASGISYGENKMYDKAFADFNTAIK
                     IDST"
BASE COUNT         1121 a          653 c          625 g         1002 t
ORIGIN      
        1 aaaatcaaca ctaaaacaaa cttcatatac agcaaatttc aatgctgtaa agtaagcaat
       61 aaccctgtgt tttagtttta agaatatggt tataaaaagt taagctataa aatgtttttt
      121 tagccgaagc accttaactt ctaccagata ataacaagca atagcaacca gccatgttag
      181 taacaggttt tgtatgggtt gctgtatcca taatttacct cccggacctg tgcctaaaaa
      241 taaaccttgc catacataaa tgccataaga tattttgcct gtaaaggcca atgggcgcca
      301 ttctaaaata gttgtaatta aactttgttg cttgctgtgt atataaccaa gcacccatgc
      361 aaaacacagt gcatgtatta agaacaaggc cgtttgcaaa ctatcgggca accaaaaggc
      421 agccagcatg gtaattacaa tgagcaattt attaaccatg ctgctgcaaa aggcaatcaa
      481 tttttcagga taaaaaaaat gtaaggccgc agctatgctt ccaatcataa tgggtgcaat
      541 ggccggtaaa aaaaaacgat tggcatggaa cgactctccg ataaaatagg acttaccatt
      601 aaatgttatg cggtacgtgg gcaaaacaat taatgccaaa attgccaaag ccaacataaa
      661 cagggcaact aacagcaggc catttttttt aaaaaacaaa accacccagg gccataaaat
      721 ataaaactgc tcttctaccg ctaacgacca tgtatgaccc aactcgctaa agtaaaggcg
      781 gttgggcaca aaattataac cgtaaaaaac cgatataaaa aaagcaaccc aactaatatt
      841 taactgatgg gttagtatta aaatgcacat ggtaaaataa aacagcacca gtaccggcaa
      901 caagcgtaaa aaccgcctga ctataaagtg tttatagttg atggtaccat ttttcgcttt
      961 ttcgcgaagc agaagtgtgg taattaaaaa gccactaatg gtaaaaaaca tggtaacgcc
     1021 aaccatgcct gttatgcttt gctccagggc attaaaaaaa taattatccg gtaaggcatc
     1081 ataccaaccc aaatgggtaa acaaaacaag taaaattgat aaagctctta acccgtcaaa
     1141 acctttaata tgaaatcccg atgtttgcat aatcaaatta ataccactta agcatggggc
     1201 aaaggtgaaa tttgttttca catttttata agacgaaaca tgcaaataag gcacgctatc
     1261 aataacagtt tattttaata cttttgcagc agaataaaaa atttaaatat catggttaat
     1321 tcaatatttc ttggcataat gggtatggga ggttcagagc tgatgatcat tctcgtactg
     1381 gtattggtta tttttggcgg ttcaaaaata ccgtcattaa tgaaaggttt aggtcagggc
     1441 attcgcgagt ttaaagatgc ctctgaacgc aaagacgata aaaaagacat tacaaaaacg
     1501 taatgagtta ttaaaaacaa aatttataat aaaggctgct gtgtaatttg catagcagcc
     1561 ttcttacatt ttatacagta cactttaagt ccgggcagtt agtttgcaat tttttagcct
     1621 gttgtttttg cttatttaag aagcaatatt caatttggca caaccgctta cctatgcccg
     1681 atacattatt tatgaaaaaa aacatagccc ctaaaaaaac tacaccatca tctattacca
     1741 caccacaaac ggcagcagct tttaaaagac ttatgttgat tgtatgcgca gtgtttgcct
     1801 ttttgcttta tgcaaataca ttaacacacg attatacagt agatgacgat accgtaatta
     1861 acaacaataa aataaccaag aaaggtgtaa gtgccctgcc cgaaattttt actactgctt
     1921 accgtgccgg attttgggaa cggaaagaaa gtttgtaccg cccgctttca gttgccatgt
     1981 ttgctgttga gtatagcatt gctcccggta atgcaacagt aggtcatata gttaacgttt
     2041 tactttatgc cgctacagca tgtgtattgt tgctggtgtt actggctttg cttaaagatt
     2101 ataatcaact attacccttt gccattacct tattgtatat agcacatccc ttgcacaccg
     2161 aagtagtagc caatattaaa agccgcgatg agttattgtg tttctttttc agcatattaa
     2221 gtatatggca catgttagct tacctggata aacaaaaaac ttcgtcactg gtatattgca
     2281 gcttttcgtt ttttttagca ttaataagta aagaaaatgc actaacattt gttgccgtag
     2341 caccattatc ggtttacttt tttacaaaag caaccggcaa acaagtgtgg caaatagcag
     2401 gtatttattt aatgattgca gttgcttact tggctttacg catagctata ttaggcggtg
     2461 caaccaactt taccgaaata cagctcatca ataattcgct tattggcgct ggcgatgatt
     2521 ggggcaaacg gctggcctct gctatttaca ttatgggccg ttacttatgg ttgttattgt
     2581 ttccacatcc gctaagtttc gattattcgt acaatacgtt tccgctagtt tcatttaccg
     2641 acttccgtgc attgttggtg ttagttataa ttggcctact gttgtattat gtaataaaaa
     2701 acttcacaaa aaaaagtccc atagcttttg ccatactgtt ttttgctgcc actatttcgt
     2761 tggtaagtaa tgtggttttt ttaattgaag ctaccctggg tgaacgtttt gcatacatgc
     2821 cctcgttggg atattgtatg gcattaccct ttgttttggc aataattttt aaagtgcaat
     2881 tacaacggca atcatatcaa agcttgcaac acatgtttgc taaaaacaaa aacatggcac
     2941 tggtattggg tgtgatactt tgcttgtact cggttaaaac agtaagccgc aacttcgact
     3001 ggaaagacaa ctttacgcta cttactaccg atgtaaaaac caataccgaa agcgcacgca
     3061 tacggtatgc attgggcagt gagtatgtac tggcgcgcgc acttaaagcc gactcgctaa
     3121 acgaagcgca gaaagcaatc gacctcgact cgggtattgt gcatttagag cgcggtgtac
     3181 aaattttacc atcttatagc gatgcatggt atcacctggc cttagcttat aaagaaaaaa
     3241 gcgaccaaac caatgccatc cggtgttttg aacaagcgcg cagcatgaag caatttaaag
     3301 aaattgattt ttatgtggca tcgggcattt catatggcga aaacaaaatg tacgataagg
     3361 catttgccga ttttaatacg gcaataaaga ttgactctac c
//
LOCUS       DAOMAA010000082         3394 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_20880_length_3394_cov_2.775681, whole genome shotgun sequence.
ACCESSION   DAOMAA010000082 DAOMAA010000000
VERSION     DAOMAA010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20880_length_3394_cov_2.775681"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..651)
                     /gene="udk"
                     /locus_tag="PLO59_01765"
     CDS             complement(43..651)
                     /gene="udk"
                     /locus_tag="PLO59_01765"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: protein motif:HMM:TIGR00235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HQU99850.1"
                     /translation="MLIIGIAGGSGSGKTTVVRQMMHSFSATQVALLSQDSYYRDLGQ
                     LEADKKRKLNFDHPDSIEFDLLVNHISQLRSGNIINQPLYDYITSSRLAETVIVKPHN
                     VVIIEGLLLFTDKRIRDLCDIKVFVDAEPDDRLIRIIERDMRERGRDAKQVIERYQVV
                     KEMHQQFIEPTKRFADLIIPQGGENKVAINLLVATISLKLMQ"
     gene            complement(833..2485)
                     /locus_tag="PLO59_01770"
     CDS             complement(833..2485)
                     /locus_tag="PLO59_01770"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HQU99851.1"
                     /translation="MKSLIKKLHLFACFALTLHTGLTTAQLNIEQPAMGHRIIEQSQH
                     SCTAHELYDALDAETNRNITQLKSEGKLIFNAGKVLLPQTFEWPLTYETNFLGNDYFF
                     IQNYVDHDTTTGSISEFMCGTRSYDLASGYDHQGTDICIGPYWWNMMDDMKVRIIAAA
                     PGQIIGKADGNFDRNCGFGAGAANSITIQHADGTRALYLHMKNGSVTTKVVGDYVETG
                     EFLGYVGSSGSSTNPHLHFEVRNAANQFIDPWAGPCNDDITSSLWNNQKPYPNSGIMS
                     ILTMSAVPTVPACPNPETVPLTNHFEPGDPMRIEIHLRDLMPGTSFELNIFDPNNVII
                     DNATVQIANFLAGGFYFTTPNISNNAIQGTYRVEVTYQGKRAAHYYTVGCPAAYNLSG
                     SIGGFQGYLSGSNITSTQSISGSGGNNIWYEAETYIEAKTGFRAKAGCHFRARIDNCS
                     NNGIRQIDELNANPMLSLQAHPTITATTTKLKVSTNKNSKLNLVVFDLTGRQIKPVIT
                     NQNLPDGVHEFELNTTDMAPGIYICTAVVDALKTTCKIIVQR"
     gene            complement(2532..>3394)
                     /locus_tag="PLO59_01775"
     CDS             complement(2532..>3394)
                     /locus_tag="PLO59_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99852.1"
                     /translation="TGNRSAVYAESKGAGTGAKYGGYFEAKNGTGLNHGVYARATGTG
                     NAYGVRSVANGQATNYGLYSVAQGNGTNYAVYGKALGLLAGLGSNYAIYGEVDASNLT
                     GWAGYFKGRGYFSERVGIGTNNPGAYLHVNADDVTLPFAVQINGASKLKTNTNGSVSI
                     GGVPSGPTNGLYVNGDVVIGTSTPAAGYKLSVNGKVMCTELRVELNNSWPDYVFNDTY
                     KLPTIKEMEQHINQYKHLPGIPSAAQMESNGGFDVGVMQTKMMEKIEELSLYIIQLEK
                     KIEKLEATHQ"
BASE COUNT          950 a          708 c          639 g         1097 t
ORIGIN      
        1 cctgattgca agcaaggttt ttattttata ttaaatctca aattattgca taagctttaa
       61 gctgatggtg gcaaccaaca aattaatagc cactttattt tcgccacctt gtggtataat
      121 taaatcggca aagcgtttgg ttggttcaat aaattgttga tgcatttctt ttaccacctg
      181 ataacgttcg ataacttgtt tcgcatcgcg gccacgctca cgcatatcac gttcaataat
      241 tctaatcaaa cggtcatcgg gctcggcatc aacaaatact ttaatatcac aaaggtcgcg
      301 tatgcgtttg tcggtaaaca gcaacaagcc ttctataatt acaacattat gcggttttac
      361 aataaccgtt tcggctaatc gcgatgaagt gatataatcg taaagtggct gatttattat
      421 atttccacta cgcaactggc ttatatggtt aaccagcaaa tcaaactcaa tactgtcggg
      481 atggtcgaag tttaattttc gttttttgtc ggcttctaat tgtcccaaat cgcggtagta
      541 actatcctgc gataaaagtg caacctgtgt agccgaaaac gagtgcatca tttgcctgac
      601 aacggtagtt ttacctgagc ccgaaccacc tgcaatacct ataataagca tattcttttt
      661 ttggcgaaat atattaattt gaataagcaa aactcttgca agtctgttgc ttgcaattgc
      721 aaaaccattc cataaaagtg tatcgctatt aattgatagc tgtaaaccta aaaaggctga
      781 atcagctttt aatgctaaca cagcctcttt acaatttcat ctaatgctaa aattaacgtt
      841 gcactataat tttgcaggtg gtttttaatg catcaacaac agcagtacaa atataaatac
      901 cgggagccat atccgtagta ttaagttcga actcatgcac accatcaggt aaattttgat
      961 ttgtgataac cggttttatc tgacggcctg ttaaatcaaa gactacaaga tttaatttac
     1021 tgtttttgtt ggtgcttacc ttcaacttgg ttgttgtagc agttatggtt ggatgtgctt
     1081 gtaacgataa catcggattt gcatttaatt catctatttg cctaatgccg ttattggaac
     1141 aattatcaat tcttgcccgg aaatggcaac ctgctttagc tctgaatcct gtttttgcct
     1201 caatataagt ttcggcctca taccaaatgt tattaccacc cgaaccggaa atgctttgtg
     1261 tagaagtgat gttgctgcct gataaatagc cctgaaaacc acctattgag ccgcttaaat
     1321 tatatgcagc cgggcaacct accgtataat aatgtgcagc acgtttacct tgatatgtaa
     1381 cttctactct atatgtacct tgtatggcat tatttgaaat gttaggcgtg gtaaaataaa
     1441 accctcctgc caaaaaatta gctatctgaa cggtggcatt atcaataatt acattattgg
     1501 gatcgaaaat gtttagttcg aatgacgtgc ccggcattaa gtcgcgcaaa tgaatttcga
     1561 tgcgcattgg gtcgcccggt tcgaagtgat tggttaaagg caccgtttcc ggatttgggc
     1621 atgcaggcac tgtgggtaca gccgacatgg taagtatgct cataatgcca ctattgggat
     1681 acggtttttg attattccaa aggctggaag ttatatcatc attacatggc ccggcccatg
     1741 ggtctataaa ttgattggca gcattgcgaa cttcgaaatg taaatgtgga ttggtagaag
     1801 atcccgaact acccacgtag cccaaaaatt cgcctgtttc aacataatcg ccaacaactt
     1861 tagttgtaac actgccattt ttcatgtgca agtaaagcgc acgtgtgcca tcggcatgtt
     1921 gtatggttat tgaattggct gcgcctgctc caaaaccaca atttctatca aagttaccat
     1981 cagcttttcc gataatttga cctggggcgg ctgcaataat tctaactttc atatcgtcca
     2041 tcatattcca ccaatatggg ccaatgcaaa tatcggttcc ctgatggtcg tagcctgatg
     2101 ccaaatcgta tgatcgtgta ccacacataa attctgatat actgcccgtt gtagtatcgt
     2161 ggtcaacata gttttggata aagaaataat cgtttcccaa aaagttggtt tcatacgtta
     2221 gcggccattc aaacgtttgt ggcagtaata ctttacctgc attaaaaata agtttgcctt
     2281 cgcttttaag ttgtgttatg tttctatttg tttcggcatc taatgcatcg tacaattcat
     2341 gtgcagtgca tgaatgttgt gattgttcaa taatgcggtg gcccatggca ggctgctcta
     2401 tgtttaactg tgctgttgtt aaacctgtat gtaacgttag tgcaaaacaa gcaaacaagt
     2461 gcaatttttt aattagtgat ttcatagttc tgtatttgtt atggaacatt tttaaaaatt
     2521 ttcattttaa atcactgatg ggttgcttct agtttttcaa ttttcttttc caattgaatt
     2581 atgtaaagcg acagttcttc gattttctcc atcattttag tttgcattac accaacatca
     2641 aagccaccat tactttccat ttgtgcagcc gaaggaatgc ctggcaaatg tttatattga
     2701 ttaatatgtt gttccatctc tttaatagtt ggcaatttgt aggtgtcatt aaaaacataa
     2761 tcaggccagc tattgtttag ctctacgcgt aactctgtac acataacctt gccgtttacg
     2821 gataacttat aacctgctgc cggagttgaa gttccgatta ctacatcgcc atttacatat
     2881 aatccattgg ttgggcccga tggtacacca ccaattgaca cgctgccatt ggtattggtt
     2941 tttaattttg aagctccgtt tatctgtaca gcaaatggca aggttacatc atcggcatta
     3001 acatgtagat aagccccggg attattggtg ccaattccta cacgctcact aaaatagccg
     3061 cggcctttaa aatagcctgc ccatccagtt aagttactgg catctacctc gccataaata
     3121 gcataatttg aacctaagcc tgctaataag cctaaggctt tgccatatac ggcataattg
     3181 gtaccattac cttgggcaac gctatatagg ccataattag ttgcctgtcc attggccaca
     3241 ctacgtacac cgtatgcgtt acctgtacct gtggcacgcg cataaacacc atgatttaac
     3301 cctgttccgt ttttggcttc aaagtaaccc ccatatttag cacctgtacc ggcacctttt
     3361 gattcggcat aaactgccga acgattgccg gtgc
//
LOCUS       DAOMAA010000083         3373 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_21115_length_3373_cov_3.261302, whole genome shotgun sequence.
ACCESSION   DAOMAA010000083 DAOMAA010000000
VERSION     DAOMAA010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21115_length_3373_cov_3.261302"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..993
                     /locus_tag="PLO59_01780"
     CDS             <1..993
                     /locus_tag="PLO59_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQU99853.1"
                     /translation="YFLYGPLANSTTSLMFEGIPHYPDAGRLWDIVDKHRVEVLYTAP
                     TAIRSLMAAGLDFVKDKNLTSLKVLGTVGEPINEEAWHWYHKYIGNGNCPIVDTWWQT
                     ETGGILISNLAGITPHKPAHATTPLPGVQPILLDDQGKEVTGNNVSGNLCIKFPWPGM
                     LRTTYGDHERCRQNYFATYAGYYFTGDGCMRDAEGHYRITGRVDDVLNVSGHRLGTAE
                     IENAINTHQGVIESAVVGFPHDIKGQGIYAYVIYQGDDVDAQANIAITVSNMIGAIAR
                     PDKIQLVAGLPKTRSGKIMRRILRKIAEGEINQLGDVTTLLDPTIVDDIKNNRQ"
     gene            complement(1005..1739)
                     /locus_tag="PLO59_01785"
     CDS             complement(1005..1739)
                     /locus_tag="PLO59_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQU99854.1"
                     /translation="MYKVIIIDDELLAQSIVVEFLSTHTLFQIVATCNNGYEGIKAIN
                     LHQPDLIFLDVQMPKINGFEMLELLDKIPAVIFTTAFDKYAVKAFETHAVDYLLKPFS
                     QERFNKAISKWLQLPGQQTKPVVDLVNNQVTENTNRLVVKEGSLVKIIAFDDVMYFEA
                     FDDYVKVFTATDCYLKKKTMSYFEQVLNPKAFLRLHRSYIVKLDLVSGIEMGDKSNHM
                     ARLSNGNLIPLSRNGYQLLKERLGLK"
     gene            complement(1732..2769)
                     /locus_tag="PLO59_01790"
     CDS             complement(1732..2769)
                     /locus_tag="PLO59_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008509196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HQU99855.1"
                     /translation="MSKYYIKNKTLIIVAGSFFAWTITHAFILQQLFLPWQAAFTDAF
                     IANAFIALTGFTINSTFKFYNPGNSGIWYRIIYLLLMSIACIWLTTLTLNYLVIEPAY
                     YPRFYKSVLPVRYMFTLLMLAFIMVVHWLLINNKQHEAHLKRKEEAELLLRDAELAKL
                     RQQLQPHFLFNSLNSISALAGSKPETARKMIQQLSDFLRSTLKKDDQKPVPLSEELAQ
                     IELYLEIEKVRFGNRLRTNLDIHPNALTCLLPPLLLQPLIENAIKFGLYDTLDEVTIT
                     VSAETKDSYLQLAITNPFDAATTNANLGTGFGLSAVSRRLYLLFGRNDLLQTTTQYNL
                     FTATLQIPLYV"
     gene            complement(2867..>3373)
                     /locus_tag="PLO59_01795"
     CDS             complement(2867..>3373)
                     /locus_tag="PLO59_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99856.1"
                     /translation="HLHRYMWPIGIILVGIYIMIKPRVNFKNNQTEWNKYEAAMGNQS
                     TGASYQSSSASDFVEASAVFGSVKKIVVSKDFKGGELNAVFGSAELNLIQADITQIVE
                     LEVNAVFGGTRLIVPTHWEIKSELTAVLGGIEDKRHIIRNPEAGSNKFLVLKGNAVFG
                     GIEILSFN"
BASE COUNT         1050 a          633 c          645 g         1045 t
ORIGIN      
        1 tattttttat acgggccgct cgccaattcc acgaccagtt taatgtttga aggcataccg
       61 cattatcccg atgccggccg tttgtgggat atagttgata aacatcgggt agaagtttta
      121 tatacagcac ccacagctat acgaagttta atggcagccg gtttagattt tgtaaaagat
      181 aaaaatctaa cttcattaaa agtattgggt actgtaggcg aacccattaa cgaagaagcc
      241 tggcattggt atcataaata tattggtaat ggtaattgcc ctatagttga tacctggtgg
      301 caaaccgaaa ccggtggcat acttatttcg aacttagcag gtataacacc gcataaaccg
      361 gcacatgcaa ctacaccatt gcccggtgtg caacctatat tgcttgatga tcagggcaag
      421 gaagtaacag gtaataacgt aagcggtaat ttatgtataa aatttccttg gcctggtatg
      481 ttgcgcacta cttatggcga tcatgaaaga tgtaggcaaa attattttgc cacttatgcc
      541 ggatattatt ttaccggtga tggatgcatg cgcgatgccg aaggccatta tcgaattacc
      601 ggtcgtgtag atgatgtatt aaatgtaagc gggcacagat taggcactgc cgaaattgaa
      661 aatgcaatta acacacatca aggagttatt gaaagtgctg tggtgggctt tccgcatgat
      721 atcaaagggc agggcatata tgcatacgtt atttatcagg gtgatgatgt ggatgcacaa
      781 gccaatattg ctattacggt tagtaatatg attggtgcca tagcacggcc cgataaaatt
      841 caattagtag ccggcttgcc caaaacccga agcggaaaaa ttatgcgcag aattttgcga
      901 aagattgccg aaggagaaat taatcagtta ggtgatgtaa ctacactatt agacccaact
      961 attgttgacg atataaaaaa taacaggcaa taaatcgcac cgtgctattt taaaccaagt
     1021 ctttctttaa gtagctgata accgttgcgg cttaggggta ttaaattacc attgctaaga
     1081 cgcgccatgt ggtttgattt gtcgcccatt tcgataccac ttaccaaatc aagtttaact
     1141 atgtaactac gatgcaggcg taaaaatgct ttggggttaa gcacttgttc aaaataggac
     1201 atggtttttt tcttcaggta gcagtctgtt gccgtaaaaa cttttacata atcatcaaat
     1261 gcttcaaaat acatcacgtc atcaaaagca attattttaa ccaaactacc ttcttttaca
     1321 accaaacgat tggtgttttc agtaacttgg ttgttcacca aatctacaac cggctttgtt
     1381 tgctgcccgg gtaattgcag ccatttgctg atagctttat taaaacgctc ctgcgaaaag
     1441 ggttttagca aataatcaac agcatgtgtt tcgaaggcct ttacagcata cttgtcaaat
     1501 gcagttgtaa atattacagc cggtattttg tctaataact ctaacatttc aaaaccatta
     1561 attttaggca tctgcacatc taaaaaaatt aaatcgggtt gatgtaagtt aattgccttt
     1621 atgccctcat atccattatt gcaggtagca acaatctgaa ataaagtatg tgtgcttaaa
     1681 aattcgacca ctatactttg cgctaatagc tcatcgtcaa ttattataac tttatacata
     1741 gagcggaatt tgtaaagtgg cagtaaacaa attgtattgc gtagtggttt gtaataaatc
     1801 gtttcggcca aaaagtaaat ataatctgcg gcttactgcc gataaaccaa aacctgtacc
     1861 caggttggca tttgtagttg cggcatcaaa cggattggtt attgcaagtt gcaaatagct
     1921 atcttttgtt tctgccgata cggtaatggt tacttcatct aacgtatcgt atagcccaaa
     1981 ctttattgca ttttcaataa gcggttgcaa cagtaatggc ggtagtaaac atgtaagtgc
     2041 atttgggtgt atatccaaat ttgtacgcag cctgtttcca aaacgtactt tttctatttc
     2101 taaatacaac tcaatttgtg caagttcttc gcttaaaggc accggtttct ggtcgtcttt
     2161 ttttaaagtg cttcgtaaaa aatcagacaa ttgctgaatc attttgcgtg cggtttcggg
     2221 cttgcttccg gccaaagcac taattgaatt aaggctgtta aataaaaaat gcggctgaag
     2281 ttgttgtctg agtttggcaa gttcggcatc gcgcaaaagc aattcggctt cttcttttcg
     2341 tttcaaatgc gcctcatgtt gtttgttatt aataagcagc caatgtacca ccattataaa
     2401 cgccaacata agtaatgtaa acatataacg tacaggtaaa accgatttat aaaaacgtgg
     2461 atagtaggcg ggctcaatca ctaaataatt taaggttagg gtagtaagcc aaatgcatgc
     2521 aatactcatt agcaacaaat aaattatgcg ataccaaata cccgaattac caggattgta
     2581 aaatttaaaa gtgctgttaa ttgtaaagcc tgtaagtgca ataaatgcat tggcaataaa
     2641 tgcatcggta aatgcagctt gccagggtaa aaataattgt tgtagtataa aagcatgggt
     2701 aattgtccat gcaaaaaaac tacctgcaac aattattaag gttttatttt taatataata
     2761 cttactcacc aaatttggta tatacatata aaactaactg tactacttgt ataaacaaca
     2821 cgcagattaa tgaatgtatc atcataatta aagtgtgtga tttggtttag ttaaaactta
     2881 aaatttcgat accaccaaaa acagcattgc ctttaagcac taaaaatttg ttgctgcctg
     2941 cctccgggtt tctgattata tggcgtttat cttcaatacc gcctaacacg gcagttaact
     3001 ctgattttat ttcccaatgc gttggcacaa ttaaacgggt acctccaaat actgcattaa
     3061 cttcaagttc tacaatttga gttatatctg cctgaatcag attaagctcg gcactgccaa
     3121 acactgcatt taattcgcca cctttaaagt ctttgcttac tacaattttt tttaccgagc
     3181 caaatacagc cgatgcttct acaaaatcgc tggcactact gctttgatag gatgcacctg
     3241 tactttgatt gcccattgcg gcttcgtatt tattccattc ggtttgattg tttttaaaat
     3301 ttacacgcgg ctttatcatg atgtaaatac caaccaatat aatgcctatg ggccacatat
     3361 aacggtgtaa gtg
//
LOCUS       DAOMAA010000084         3364 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_21204_length_3364_cov_3.181021, whole genome shotgun sequence.
ACCESSION   DAOMAA010000084 DAOMAA010000000
VERSION     DAOMAA010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21204_length_3364_cov_3.181021"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2139)
                     /locus_tag="PLO59_01800"
     CDS             complement(<1..2139)
                     /locus_tag="PLO59_01800"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HQU99857.1"
                     /translation="MWNYFANKLLRNRLAFILVLGALTAFMAYRATQIELSYEFAKVL
                     PATDPAYADYENFKKMFGEDGSVMVIGLQDTNLFNLNVLNDWYALSNDIKSIDGVQDV
                     LSLTTLYNVIRNDSLQRFDFKPVMTKPLQTQAEADSLRNLIYNLPFYNDLVINQKTNA
                     TLIAITFKKVDLNSKHRIQMVEQIKAVSQKFEAGHQLKLHYSGMPYIRTAIMNKVSGE
                     MKLFLILGIIITTIILGLFFKSFTSVVFSVVVVIAGVIWSMGTIELLNYKITILTGLI
                     PPLIMVIGVPNCIFLINKYHSEFALHGNKVKALTRMITTIGVSLFLANITTSIGFGVL
                     YFTNSSFLTEFGVIAAVNVMLTYIITLIFIPIILSYLPVPNAKDLIHLEAKRINFVLD
                     IIDTLVHRYRNWIYGVTAVVTAVFIYGMMQINMNGFVVDDLPADDPVYADLRFFESNF
                     KGILPFEIYIDTKKPNGVFENNARVLYKIKTLQKTLEQYPELSRALSVTEAIKFSYQA
                     YRGGDKKFYVLPGISELKNLNDYVATVKGGDNRLKSFIDSTKQYTRVSIQMADVGSVR
                     IKELLTEIKPRIDSIFDNNNYHVKLTGHSLVFLKGNDYLLHNLLESLLIEIVLIALVG
                     LALFRSTRIILLSKLPCLIPLLITAGIMGFMGIRFKPSTILIFSIAFGIASDGTIYFL
                     AKYRLELKQHRKSIADAVSAAIKDTGLSMIY"
     gene            2513..>3364
                     /locus_tag="PLO59_01805"
     CDS             2513..>3364
                     /locus_tag="PLO59_01805"
                     /inference="COORDINATES: protein motif:HMM:NF015478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99858.1"
                     /translation="MNNTGTATLLQTIVEILNNNTNETLMQLELNSLAAYCSDGMEHD
                     SEKFLAAILIHAINRKFNPELLNNNIYDKQYEVSQIELFDILIDKFPFVKYGQQMVNN
                     SIIETMSHYDEVTLVDIGIGLGTQMVNIISMASGLSNLKKVTIVGIEPFEDALNIAEA
                     NIKAIKNTAHFEIEIVKIADYAENVNFNSIPKLQGAVIVNASLALHHIQQKAQRLVTL
                     QRIKAINPVAFFITEPNVDHFEPDYKQRINNCYHHFIALFKVIDNLDIERKYKNGLKL
                     FFGREIED"
BASE COUNT         1153 a          607 c          582 g         1022 t
ORIGIN      
        1 ataaatcata cttaaaccgg tatcttttat tgcagcggaa actgcatcgg caatactctt
       61 acgatgttgt tttaactcaa ggcgatactt agccagaaaa taaatagtac catctgatgc
      121 aatgccaaaa gcaatactga aaattaatat tgtggatggt ttaaaccgta tgcccataaa
      181 acccattatg ccggccgtaa ttaaaagcgg tataaggcaa ggtagttttg acaataatat
      241 aatccgggtc gaacgaaaca atgcaagacc aacaagtgca atgagcacaa tttcaattaa
      301 aagactttcg agcaagttgt gcaacaaata atcgttacct tttaaaaaca ccaaactgtg
      361 gccggttaat tttacatggt aattgttgtt gtcaaaaatc gaatcaatgc gcggcttaat
      421 ttcggtaagc agttctttaa ttcttaccga acccacatca gccatttgaa tacttacacg
      481 tgtgtattgt ttggtcgagt caataaacga tttcaatctg ttatcgccac cttttacggt
      541 ggctacataa tcattaaggt tttttaattc gctaatgcct ggtagtacat aaaacttttt
      601 atctccgccc ctgtatgctt ggtatgagaa ttttatagct tcggtaactg acaaagctct
      661 ggatagttcg ggatattgtt ctaatgtttt ttgaagagtt tttattttgt aaagtacacg
      721 tgcattattt tcgaaaacac cgttgggctt tttggtatca atataaattt cgaatggcag
      781 tatgccttta aaattcgact caaaaaaacg aaggtcggca taaaccgggt catcagcagg
      841 caagtcgtca accacaaaac cattcatgtt tatttgcatc atgccataaa taaaaactgc
      901 ggtaacaaca gcggttactc cataaatcca gttacgatac cggtgtacga gcgtatcaat
      961 aatatcaagc acaaagttta tgcgtttagc ctctaaatgg atcaaatctt tagcatttgg
     1021 caccggcaaa tagcttaata taatcggaat aaaaataagc gtaataatgt aggtaagcat
     1081 tacattaaca gcagcaataa caccaaactc ggtaagaaag gagctattgg taaagtacaa
     1141 tacaccaaaa ccaattgaag tggtaatgtt tgccaaaaac aatgatacac ctatggtagt
     1201 aatcatgcgt gttaaggctt ttactttgtt gccgtgcaag gcaaattcgc tatgatattt
     1261 attgataaga aaaatacaat taggaacgcc aattaccata atcagtggcg gaattaaacc
     1321 ggttagtatg gtaattttat aattaagcag ttcgatagtg cccattgacc agattactcc
     1381 ggcaataacc accaccactg aaaaaacaac tgaagtaaaa cttttaaaaa acagccctag
     1441 aataatggtt gtgataatta ttccaagtat taaaaataat ttcatctcgc ccgaaacctt
     1501 gttcataata gccgtacgga tgtaaggcat accggagtaa tgcagcttta attgatgccc
     1561 ggcttcaaac ttttgtgaaa ccgctttaat ctgctccacc atttgtatgc ggtgcttcga
     1621 atttaaatca acctttttaa aagtaatagc aataagtgtg gcattggttt tttggttaat
     1681 caccaaatcg ttgtaaaaag gtaaattgta aattaagtta cgtaaactat cggcttctgc
     1741 ttgtgtttgc agcggcttag tcataacagg tttaaaatcg aagcgctgta aactatcgtt
     1801 acgaattaca ttgtaaagcg tggttagtga taacacgtct tgcacgccat caatactttt
     1861 tatgtcattg ctaagggcat accaatcatt aagcacattt aaattaaaca agtttgtgtc
     1921 ttgaaggcca ataaccataa cgctgccatc ttcgccaaac atttttttaa agttctcgta
     1981 atcagcataa gccggatcgg tggccggcaa aaccttagca aattcataag aaagttctat
     2041 ttgggtggcg cggtaggcca taaaagcggt taaagcaccc agcaccagaa taaaagctaa
     2101 ccggttacgt aataatttat tggcaaagta attccacata gttcaattgt gttgcaaata
     2161 cttttatctc aacatgatgt ctaaacaaat ttaaaataca atgcgtttag tgtttaacta
     2221 attaaaaagg tatttgcatg taacggtgca aataaaatca atatcccatt tttaacaata
     2281 cctattcctt gagttaatct gttttagtat gaatctaaaa ttgcttcaaa aaaaacagca
     2341 agcattttat gctgtttagg gaaggctttt agtgttttta aacgtagatg taatagcact
     2401 acatcactaa aataatacac ctcagttcca ttttaccacg ttttaggcaa caataatttt
     2461 gtcggggttt gtgcattgta tgtttgtatc cataaaccaa ataaactaac aaatgaacaa
     2521 caccggcaca gctaccttac tacaaaccat tgttgaaata ttaaacaata atacaaatga
     2581 aaccttgatg caattggagc ttaattcatt agcagcatac tgtagtgatg gtatggagca
     2641 tgattctgaa aaatttttag ctgccatttt aatccatgca atcaatcgga aatttaatcc
     2701 tgaattacta aataataaca tctatgataa acaatatgaa gtttcgcaaa tagaattgtt
     2761 tgatatacta attgataaat ttccatttgt aaaatacggc caacaaatgg taaacaattc
     2821 aattatcgaa accatgagcc attatgatga agtaacactg gttgatattg gcattggctt
     2881 gggtacccaa atggttaaca ttatcagtat ggcctcaggt ttatccaacc ttaaaaaagt
     2941 aaccattgtt ggcattgagc cttttgaaga tgccttaaat attgccgaag ccaatattaa
     3001 ggcaataaaa aacaccgctc actttgaaat tgaaattgta aaaatagctg actatgccga
     3061 aaatgtaaat tttaattcca tacccaaact gcaaggcgct gtaattgtaa atgcatcatt
     3121 ggctttgcat catattcagc aaaaagcgca acggctggtt accttacaac gcattaaggc
     3181 cattaacccg gttgcatttt ttattacaga acctaatgta gatcattttg aacccgacta
     3241 taaacaacgc ataaacaact gttaccacca ctttattgcc ttgtttaaag ttattgataa
     3301 cctggacatt gaacgtaagt ataaaaacgg attgaagtta ttttttggcc gtgagattga
     3361 agat
//
LOCUS       DAOMAA010000085         3351 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_21351_length_3351_cov_3.588896, whole genome shotgun sequence.
ACCESSION   DAOMAA010000085 DAOMAA010000000
VERSION     DAOMAA010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21351_length_3351_cov_3.588896"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="PLO59_01810"
     CDS             complement(<1..228)
                     /locus_tag="PLO59_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535523.1"
                     /note="Sms; stabilizes the strand-invasion intermediate
                     during the DNA repair; involved in recombination of donor
                     DNA and plays an important role in DNA damage repair after
                     exposure to mutagenic agents; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HQU99859.1"
                     /translation="MSKVKTVFYCQSCGAQSPKWVGKCNQCGQWNTLVEEIISTPKTN
                     AWTDKNKPTNKPVLLQDVADNNTQRTTLPDAE"
     gene            complement(279..719)
                     /locus_tag="PLO59_01815"
     CDS             complement(279..719)
                     /locus_tag="PLO59_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99860.1"
                     /translation="MKRTLPVVIFLVAVIILAGCDSKTFLRSKKKYESDLQGYWRPIK
                     GTSIYGTSVHDQNVQWKFESGSLEIVRVNSNGSETLLDKGNYSIDTKIDQSFLTTSGF
                     TSRDYDTILDFNLKWTIIQLNNDILDITGRPNNGGQIEIEFYKQ"
     gene            complement(768..1271)
                     /gene="rfaE2"
                     /locus_tag="PLO59_01820"
     CDS             complement(768..1271)
                     /gene="rfaE2"
                     /locus_tag="PLO59_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016195189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HQU99861.1"
                     /translation="MRIDTHQKFFAPIMGNMHLLQPLIASWHNLQKKIVFTNGCFDLL
                     HLGHVDYLSKAAALGDVLVIGLNADASVSRLKGPARPITDEKSRGLILCALAFVDAVV
                     WFGADTPYELIKLVKPHVLVKGADYEPHKIVGYDIVTGNGGTVKTIDFLPGYSTSLIE
                     KKIKSQI"
     gene            1447..3171
                     /gene="lysS"
                     /locus_tag="PLO59_01825"
     CDS             1447..3171
                     /gene="lysS"
                     /locus_tag="PLO59_01825"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HQU99862.1"
                     /translation="MSQLSEQELQRRQKLNELNQLGINAYPAPLFDVNFSSAEILKKF
                     ETNALDAKLQHISIAGRLMSVRDMGKASFFVLQDAEGKIQAYIKRDDVSKDGDTTTYD
                     VLFKKLMDIGDIIGITGYAFRTKMGEISIHVTGLTMLSKALRPLPVVKEKDGQVYDAF
                     SDAEQRYRQRYVDLIVNPHVRDTFKKRTQLMQVMRTFLDQRGYLEVETPILQPLHGGA
                     AARPFKTHHNTLDMTLYLRIANELYLKRLIVGGFDGVYEFSKDFRNEGMSRFHNPEFT
                     QIELYVAYKDYYWMMDLVEELIEKIAITLHGTTHVKVGDNEINFARPWKRFTMYEAIA
                     HYTGIDITDMDETEMRRQCQKLGIKVDDTMGRAKLIDEIFGETVEHKLIQPTFIMDYP
                     QEMSPLTKKHRSKPGLVERFEAICNGKEICNAYSELNDPIDQRLRFEEQLELAKRGDE
                     EAMQLDEDFLRAIEYGMPPTAGIGIGIDRLAMIMTNSASIQDVLFFPQMRPEVIGSSQ
                     SDVANTLQHIPEAWQAVLGKLGITDMVQLRAQNQNKLFNDMGGMRKKLKLDIPALTLT
                     HLQQWITT"
BASE COUNT         1054 a          642 c          660 g          995 t
ORIGIN      
        1 ttcggcatcg ggcagggtgg tacgctgtgt gttattatcg gctacatctt gtaataatac
       61 aggcttattg gtgggtttgt ttttatcggt ccaggcattg gttttagggg tgcttataat
      121 ttcctcaact aaggtattcc attggccgca ctggttgcat ttgcccaccc atttaggcga
      181 ttgtgcgcca catgattggc aataaaaaac ggttttaact ttcgacaatc ggtaggtggt
      241 tttaaattct tttgctcatt gcttgaaatc gaaacaggtt attgtttgta aaattcaatt
      301 tcaatttggc caccattatt gggtcggcct gttatatcca gtatgtcatt gttaagctga
      361 atgattgtcc acttcaaatt aaaatctaaa atggtatcat agtctctgct ggtaaatcca
      421 ctggtagtta aaaaactctg atcaattttt gtgtcaatac tataattgcc tttatctaac
      481 aatgtttcgg aaccattgct gttaacacgt acaatttcta agcttccact ttcaaacttc
      541 cattgcacat tttgatcatg tacggatgta ccataaatag atgtgccttt tataggacgc
      601 caataacctt gcaaatcaga ttcgtatttt tttttcgagc gtaaaaatgt tttcgagtcg
      661 caaccggcaa gtattattac tgctactaaa aaaattacaa caggtaaagt tcttttcatg
      721 tcagtgcgtt ttttaatata ttcaaatgta aataattgaa ggcaatttta aatttgactc
      781 tttatttttt tttcaatcaa tgaagtggaa taacccggta aaaaatcaat ggttttaacc
      841 gtacctccat ttccagttac aatatcataa ccaacaattt tatggggctc atagtcggca
      901 cctttaacca atacatgcgg tttaaccagc tttattaatt cataaggcgt atccgcgcca
      961 aaccatacta cggcatctac aaaagccaaa gcacataaaa ttaaacccct gcttttttca
     1021 tcggttatgg ggcgtgcggg gcctttcagt ctgcttaccg atgcatcggc atttaggcct
     1081 attaccaaaa catcgcctaa agcagccgct ttgcttaaat aatcaacatg ccctaagtgc
     1141 agcaaatcaa aacagccatt ggtaaaaact attttttttt gcaggttatg ccaactggca
     1201 ataagcggtt gtaatagatg catattgccc attatgggag caaaaaattt ttgatgggta
     1261 tctatgcgca tagcaaagta aatgagctgc aaaagtatga aatgactttc ggatttaaag
     1321 cacacatacg cttaccgtca gttgttatac ttacgacaaa aacataccgt ttagatgcaa
     1381 caaattttac atttgcacgc ctcaatctaa aatggaattg ggtatttaaa taacaagcaa
     1441 gtacgcatga gtcaattatc cgaacaagaa ttacaaagac gacaaaaact taatgagcta
     1501 aaccagttag gcattaatgc ttacccggca ccattgtttg atgtaaattt ttcatctgcc
     1561 gaaattttaa aaaaatttga aaccaatgcc cttgatgcca aattgcagca tataagtatt
     1621 gcaggcaggt taatgagtgt gcgcgatatg ggcaaagctt ctttttttgt tttacaagat
     1681 gccgaaggaa aaatacaggc ttatataaaa agagatgatg taagtaaaga tggcgatact
     1741 actacctacg atgtactgtt taaaaagcta atggatattg gtgatattat tggcataacc
     1801 ggctatgctt ttcgtactaa aatgggcgaa ataagtattc atgttacggg cttaaccatg
     1861 ctctcgaagg cattgcgccc cttgccagtg gttaaagaaa aagacggaca ggtttatgat
     1921 gcgtttagcg atgccgagca acgttaccgc cagcgttatg ttgatttaat tgtaaacccg
     1981 catgtacgcg atacatttaa aaagcgtacg caactgatgc aggtaatgcg cacgttttta
     2041 gaccaaaggg gttatctcga agtagagacg cccatactac aacccttaca tggtggtgct
     2101 gcagcgcgtc cgtttaaaac gcatcacaat acgttggata tgactttgta tctgcgtata
     2161 gccaatgagt tatatttaaa acgacttatt gtaggtggct ttgatggtgt gtatgagttt
     2221 tcgaaagatt tcagaaacga aggtatgagc cgttttcata acccggagtt tacacaaatt
     2281 gaattgtacg tagcatacaa agattattat tggatgatgg atttggtaga agaacttatc
     2341 gaaaaaattg ccataacact gcatggaact acgcatgtaa aagtgggtga taatgaaata
     2401 aattttgcac gtccctggaa acgctttacc atgtatgaag ccattgcaca ttatacaggc
     2461 attgatatta ctgatatgga cgaaacagaa atgcgcaggc aatgccaaaa attaggtatt
     2521 aaagttgatg ataccatggg acgcgctaaa cttattgacg aaatttttgg cgaaaccgta
     2581 gagcataaat taatacagcc aacttttatt atggattatc cgcaggaaat gagcccgctt
     2641 acaaaaaaac acagaagtaa gcccggcttg gttgagcgat ttgaggcaat ttgtaatggc
     2701 aaagaaattt gtaatgctta cagcgagtta aacgacccca ttgaccagcg cctaagattt
     2761 gaagaacaac tggaacttgc caaacggggc gatgaagaag ccatgcaatt agacgaagat
     2821 tttttgcgtg ccattgaata tggtatgcca cccacagccg gtattggtat tggtatcgac
     2881 cgtttggcca tgataatgac aaattcagcc tcgatacaag atgtactgtt ttttccgcaa
     2941 atgcgccccg aggttattgg cagcagccag tccgatgttg ccaatacatt gcaacatata
     3001 cccgaagcat ggcaagctgt tttaggcaaa ctgggtatta ccgatatggt tcagttacgt
     3061 gcacaaaacc agaacaaact ttttaacgat atgggtggga tgcgtaaaaa attgaaatta
     3121 gatatcccgg cacttacgct tacacatttg cagcaatgga taacaacata aaagcacctt
     3181 attttgaaag caccacaatc aaatatttat ggatcctgtt tcagaaaaag tcaacctatt
     3241 gcaggctaac acaactaacc gctatccact aaccactaat cactccttca caaacctgcc
     3301 ataatacatt ttatcgcctt gctgcacttg taatacgtat aagcccggtg t
//
LOCUS       DAOMAA010000086         3342 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_21456_length_3342_cov_2.546091, whole genome shotgun sequence.
ACCESSION   DAOMAA010000086 DAOMAA010000000
VERSION     DAOMAA010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21456_length_3342_cov_2.546091"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1257
                     /locus_tag="PLO59_01830"
     CDS             <1..1257
                     /locus_tag="PLO59_01830"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HQU99863.1"
                     /translation="SFNGFFNQDIKLPEQPDVTNEVINGVQFKVATIKKVVLFPQQTG
                     LLNIDEMKGQCVARIQVKKQRSYNDPFDIFNDPFFTGGAQDQSINIKSDPVRITVKDF
                     PTTPPASFKGAVGVFDFTATLDKTDVKENEPINLKVRISGKGNLKLLENPEVKLPPQL
                     ETYDPKIAEKITVADGGSSGSKAFDYLIIPRTAGNYEIPPIEFTYFDLNRKAFVTHTS
                     GKLLAKVAKGDGSVTATVYDNSVNKTEVAVLGKDIRYIKQDATFTSNHNSFYGSTLFY
                     ASVAMPILLLIIGGIYIQKQQALNNNFALVKSKGANKLAVKRLATANKYLAAKQNHAV
                     YDEVYKALTLYLSDKLNIPMADLNKDHAATMMSLRKVSEATIKQWVTSLSACEMARFA
                     PSAVASPQQIYNDAIGIITKIENEIV"
     gene            1244..2011
                     /locus_tag="PLO59_01835"
     CDS             1244..2011
                     /locus_tag="PLO59_01835"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQU99864.1"
                     /translation="MKLYKRILPLWLCIIAFCCLGNSTFAQSNEVKNANSYYSQDKFE
                     EAAIAYEKILKDFGASAQIYYNLGNAYYKTGKYEFAILNYERALKLNTDEDVQYNLKL
                     ANMQCVDNIEPVPQVFYQKWWQQLMQQSNADVSAAWMIAALFGFSILLLLFLMLQKPM
                     IKRICFFSAGALLVFAALQYTLANRQNAYMQNNKAAILMKVSDYVKSSPDTKSANLFM
                     LHAGTKVTVLDSLNGWHKIKIANGHVGWIDSKAIEII"
     gene            2073..>3342
                     /locus_tag="PLO59_01840"
     CDS             2073..>3342
                     /locus_tag="PLO59_01840"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HQU99865.1"
                     /translation="MHFKSYLKYFSLTHYIRNKWLLWFVRCMLALVFYIFLLESNFLF
                     ITGPVPALKDIKNPQVAIASDIYSADSVLLGRYYIENRTPVSYNEMSPWLTSALVATE
                     DARFYKHHGLDLFALAGSVWQTAQGDQRGGSTITQQLVKNIFKTRAVASQGLLRHVPG
                     VRTFVYKTKEWLTALKLEFYFSKNDILELYLNSVDFGNNWFGVKVASNNYFSKPPLKL
                     QAEEAAMLIGMLKATTTYNPKRNKTKSLQRRNVVLAQMAKYNYISVQQSDSLQALPLK
                     LNLRKLKKTESDDSYIRQYVEKLITPWCIQNKLNLYEDGLKIYTTINAKLQRIAEESL
                     TIHTQKLQKQFNEQWRGKKPWIDDNGVEIKNFIEQQLEQTTAYADLMQQFKNNSDSAH
                     LYLHKTNPMKIFSWKGPIDTTFSSYDSLKYY"
BASE COUNT         1154 a          637 c          617 g          934 t
ORIGIN      
        1 tcatttaacg gcttttttaa tcaggatata aaactacccg aacaacctga tgttactaat
       61 gaggttataa acggtgtaca atttaaagtt gcaacaatta aaaaagttgt actgtttcca
      121 cagcaaaccg gcttgctaaa cattgatgaa atgaaaggcc aatgcgtagc acgcattcag
      181 gtaaaaaaac aaagaagcta caacgaccca tttgatattt ttaatgatcc gttttttacg
      241 ggcggtgcgc aagatcaaag tatcaatatt aaatcagacc cggttcgtat taccgtcaaa
      301 gattttccaa caacaccacc tgcaagtttt aaaggcgctg taggcgtatt cgattttaca
      361 gcaacgctcg acaaaaccga tgtaaaagaa aatgaaccta tcaatttaaa agtacgcatt
      421 tcgggcaaag gcaatttaaa actactcgaa aaccctgaag taaaattacc gccacagtta
      481 gaaacatacg acccaaaaat tgccgaaaaa attacggttg ccgatggtgg ctcatcaggc
      541 agcaaagctt ttgattacct gattatacca cgcactgccg gtaattacga aatacctcca
      601 attgaattta cctactttga tttaaaccgt aaagcatttg ttacacatac atcgggcaag
      661 cttttggcta aggttgccaa aggcgatggc agtgtaacag ctactgtata tgacaacagt
      721 gtaaataaaa ccgaagtggc cgttttgggt aaagacatca gatacataaa acaagatgca
      781 acctttactt caaaccacaa ttcgttttat ggctctacac ttttttatgc atcggtagca
      841 atgcccattt tgttactcat tattggcggc atatatatac aaaaacaaca agcattaaac
      901 aacaactttg ctttggtaaa aagtaaaggc gctaataaat tggcggttaa acgattagct
      961 acagccaaca aatatctggc agcaaaacaa aaccatgcag tttatgacga agtatataaa
     1021 gcacttacac tttatctcag cgataaatta aatataccca tggccgattt gaataaagac
     1081 catgctgcaa caatgatgtc gttacgcaaa gtaagtgaag caactattaa gcaatgggta
     1141 acttcgttaa gcgcatgtga gatggctcgt tttgcgccaa gcgctgttgc ttcgcctcag
     1201 caaatttata acgatgccat tggcataatt accaaaattg aaaatgaaat tgtataaacg
     1261 tattttacca ttatggcttt gtatcattgc attttgctgc ctgggcaata gtacctttgc
     1321 acaaagcaat gaagttaaaa atgcaaatag ttattacagt caggataagt ttgaagaagc
     1381 cgcaattgct tatgaaaaaa tattgaaaga ctttggcgca tcagctcaga tttactataa
     1441 cctgggaaat gcatactaca aaacaggcaa gtacgaattt gcaattttga actatgaaag
     1501 agcgcttaaa ctaaatactg acgaagatgt acaatacaat ttaaaactgg ccaacatgca
     1561 atgtgtggat aatatagagc cggtgccaca agtgttttat caaaaatggt ggcagcaact
     1621 tatgcaacaa tccaatgcag atgtaagtgc tgcatggatg attgccgcat tgtttgggtt
     1681 ttcaattctg ctactattat ttttaatgct acaaaaacca atgataaaac gcatttgctt
     1741 ttttagtgcc ggtgctttgt tggtttttgc tgccttacaa tacacattag ctaaccggca
     1801 aaacgcatac atgcaaaata ataaggctgc tattttgatg aaggtgagcg attatgtaaa
     1861 gagttcgccc gataccaaat cggccaattt atttatgctg catgcgggta ccaaagtaac
     1921 ggtgctcgat agtttaaatg gctggcataa aataaaaata gcaaatgggc atgtgggctg
     1981 gattgactca aaagccattg aaataattta agtctgcacg ataatattga atatcgtaat
     2041 gctgcattgc atttttgttt taattgcatg ccatgcactt taaatcctat ttaaaatatt
     2101 tttcgcttac gcattatatc cgcaataaat ggttgctatg gtttgtgcga tgtatgttgg
     2161 cattggtttt ttatattttt ttattagagt caaatttctt attcataaca ggaccggtac
     2221 ctgcactaaa agatataaaa aatccgcagg tagcaattgc atctgatatt tatagtgccg
     2281 atagtgtttt actgggccgc tactacatag aaaaccgtac accggttagc tataacgaaa
     2341 tgtcgccctg gcttaccagt gccttagttg ccaccgaaga cgcacggttt tacaaacatc
     2401 atggtttaga tttgtttgca cttgccggca gtgtttggca aacagcacaa ggcgatcagc
     2461 gaggtggcag taccattaca cagcaattgg taaaaaatat ttttaaaaca cgggctgtag
     2521 caagccaagg tttacttagg catgtaccgg gtgtacgcac ttttgtttat aaaaccaaag
     2581 agtggcttac tgcattaaaa cttgaatttt atttcagtaa aaacgatata ttagagttgt
     2641 acttaaactc agttgatttt ggcaacaact ggtttggcgt taaggtagcc agtaacaatt
     2701 attttagtaa gccaccattg aaactgcaag ccgaagaagc cgccatgcta attggcatgt
     2761 taaaagctac tactacctac aaccctaaac gcaataaaac aaaatcgcta cagcgaagaa
     2821 atgtggtact tgcccaaatg gccaaataca attatataag tgttcaacaa tccgactcgt
     2881 tacaagcatt acccttaaaa ctcaacctgc gaaaattaaa aaagacagaa agcgatgact
     2941 cttacatccg tcaatatgtc gaaaaactga taacgccctg gtgtattcaa aataaactga
     3001 atttgtatga agacggctta aaaatataca ctaccatcaa cgccaagtta caacgcatag
     3061 ccgaagaatc gcttacaata catacgcaaa aactacaaaa acaatttaac gaacaatggc
     3121 gtggcaaaaa accgtggatt gatgacaatg gtgtggagat taaaaatttt atcgaacagc
     3181 aactagaaca aactacggcc tatgctgatt tgatgcagca gtttaaaaac aattccgatt
     3241 cggcacattt atatttacat aaaaccaacc ccatgaaaat attttcgtgg aaaggcccca
     3301 ttgatacaac attttcaagt tacgattcat taaaatacta cg
//
LOCUS       DAOMAA010000087         3336 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_21522_length_3336_cov_2.806157, whole genome shotgun sequence.
ACCESSION   DAOMAA010000087 DAOMAA010000000
VERSION     DAOMAA010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21522_length_3336_cov_2.806157"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /gene="pyk"
                     /locus_tag="PLO59_01845"
     CDS             complement(<1..837)
                     /gene="pyk"
                     /locus_tag="PLO59_01845"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF012449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HQU99866.1"
                     /translation="MKFNRTKIVATIGPASQSAEVLENMIDAGMNVCRINASHGSHEQ
                     QQLIIDTLRNLNAKKNVHTALLYDLQGPKLRIGDMRDGGVIIKEGDEIILTTNDCIGD
                     EKRVFIKYASFVKDVKKHDTILVDDGKIELEAIQSNGIDEVVTKVKFGGMLSSRKGVN
                     LPNTRITLPSLTEKDKADLEFALANNIDWIGLSFVRSAKDIEALKSIIKYKNSPMRVV
                     AKIEKPEALKDLDNIISSADGVMVARGDLGVEIPMEQVPLIQKTILNKCIAKSKPVII
                     ATQ"
     gene            complement(885..1316)
                     /locus_tag="PLO59_01850"
     CDS             complement(885..1316)
                     /locus_tag="PLO59_01850"
                     /inference="COORDINATES: protein motif:HMM:NF033205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPExxxVDY family protein"
                     /protein_id="HQU99867.1"
                     /translation="MPRKILKINYDDELNFSLMGMATGLKDYRLAFEMNKALEIDLQR
                     AADHILIVGKNNSQIRYNRFQYFDSIGLTYTLISNKNQSSLMLPELQMIDYLLLLEPP
                     DENTAKEIIISLKKIKTIQAVFKFEIKDLKSRQNLLSETAD"
     gene            complement(1385..2131)
                     /locus_tag="PLO59_01855"
     CDS             complement(1385..2131)
                     /locus_tag="PLO59_01855"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HQU99868.1"
                     /translation="MQTIKIIAGNIWRCWFFFNAVLIFFLLYPFVVFYIRKPTGYPMV
                     FKLKKIWAGYTLFVSGINCIVVQPVKLDKNLAYIFCPNHASVLDIFISYKAIPQYFHF
                     MGKAELKRIPFFRIFFKDMNIAVDRDSIKASYGAYARAESDLKKGISLGIFAEGTIPA
                     CTPRLGGFKNGAFKLAIENEVAIVPVTFVNTWKILPDVNRKFGGRPGTVTVHIHKPIS
                     TQTIALNRLPELRDAVRNKIQEPLRPYYIM"
     gene            2272..3264
                     /gene="trpS"
                     /locus_tag="PLO59_01860"
     CDS             2272..3264
                     /gene="trpS"
                     /locus_tag="PLO59_01860"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008240965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HQU99869.1"
                     /translation="MKDIVVSGIRSTGNLHLGNYFGAVANFIKMQHQHPCFFFVADYH
                     SLTTHPNPVDLLANERQVIIEYLACGLDPEICTLYLQSDIPQTAELYLFLNMLAYLGE
                     LERVTTFKDKARKHPDNINAGLLTYPTLMAADVLIHKGAKVPVGKDQEQHLEMMRTFG
                     NRFNRLYNVDYFPEPQAFNFGTELVKVPGLDGSGKMGKSEGEGNALFLCDDDKTIRKK
                     IMRAVTDSGPTQLNQPKPDAIKNIFALMNLVSAKDTVNYFDEQYNNMTIRYGDLKKQL
                     AEDMVLFINPLAQKISDLKQNDDYVTKVIAMGKEKAAASANKTITEVRKIIGFK"
BASE COUNT         1030 a          589 c          597 g         1120 t
ORIGIN      
        1 ttgcgttgca ataattaccg gttttgattt ggcaatacat ttattcaaaa tggttttttg
       61 aatcagcggt acttgctcca tcggaatttc aacacctaaa tcgcctcgtg caaccattac
      121 accatctgcc gatgaaatta tattatccag gtcttttaac gcttcgggtt tttcaatttt
      181 cgcaaccacg cgcatgggac tgtttttata tttgataata ctttttagtg cttctatatc
      241 ttttgccgac ctaacaaaag acagtccaat ccaatcaata ttgttagcca atgcaaactc
      301 taaatcagct ttatcttttt cggtaagcga tggaagtgta atacgtgtat tgggtaggtt
      361 aacgcccttg cgtgatgata acataccacc aaattttact ttggttacaa cttcatcaat
      421 gccattgctt tgtatagctt ctaattctat tttaccatca tctactaaaa tggtatcatg
      481 tttttttaca tctttaacaa aggatgcata cttaataaaa acacgttttt catcaccaat
      541 acaatcgttg gttgttaata ttatctcatc accttctttt ataattacac caccatcgcg
      601 catatcgcca atacgtaatt tggggccttg caaatcataa agcaatgcag tatgtacatt
      661 tttttttgcg tttaaattcc gcagcgtgtc tattattaat tgttgctgtt catgactgcc
      721 gtgcgaagca tttatgcggc aaacattcat accggcatca atcatgtttt caagtacttc
      781 ggcactttgc gatgcagggc cgatggtggc cactattttg gttctattaa atttcatgtt
      841 ttttaattga ttgatttctg atagtattta tcagctcttg ttttttagtc agcggtttcg
      901 ctcaataaat tttgacgtga ttttaaatcc ttaatttcaa atttaaatac ggcttgtatg
      961 gtctttattt tttttagtga tatgattatt tctttggctg tgttttcgtc aggtggttct
     1021 aatagcaata agtagtctat catctgcaat tcaggtaaca tcaagctgct ttgattttta
     1081 ttgctgatta aagtgtaagt taaacctatt gaatcaaaat actgaaaccg attgtatcga
     1141 atttgcgaat tgtttttacc tacaattaaa atatggtcgg ctgcacgttg taaatcaatt
     1201 tccaaggctt tattcatttc aaaagcaagc cgatagtctt taagcccggt agccataccc
     1261 attaatgaaa aatttaattc atcatcgtaa ttgattttta aaattttgcg gggcattaat
     1321 taaatgtatg gtattaatta agtgtcaaaa gtacactaag ggttgtaaaa gcaaaacata
     1381 tcttttacat aatgtaatag gggcgtaatg gttcttgtat cttattacga acggcatcgc
     1441 gcaattcggg taaccggttt aatgcaattg tttgtgtgct tattggttta tgaatatgta
     1501 cggttaccgt accgggcctg ccaccgaatt tacggttaac atcgggcaat attttccagg
     1561 tgtttacaaa tgtaacgggt acaatggcca cctcattttc gatggcaagt ttaaatgctc
     1621 catttttaaa accgcctaaa cgtggtgtac atgcaggtat ggtaccttca gcaaaaatac
     1681 ccagactaat accttttttt aaatcggact ctgcccttgc atacgcccca taggatgcct
     1741 ttatactatc gcgatcaacg gcaatattca tgtccttaaa aaaaatacga aaaaatggta
     1801 tccgttttaa ttcggccttt cccataaaat gaaaatattg tggtattgct ttatatgaaa
     1861 taaaaatatc aagcacgctt gcatgattgg gacaaaaaat atatgcgagg tttttatcca
     1921 atttaaccgg ttgcaccact atacaattaa tacctgaaac aaacaaagta taaccggccc
     1981 aaattttttt aagtttaaaa accatcgggt aacctgtcgg ttttctgata taaaaaacta
     2041 cgaatggata tagcagaaaa aaaatcagca cggcattaaa aaaaaaccaa caacgccaaa
     2101 tattaccggc tatgatttta attgtttgca taaaagttgt tgctgttaag tagtttcaaa
     2161 tgaacaaata attgccttag gtaattataa aatatccaaa gcacattggt ttattattga
     2221 tgcataatta tattttcgcc tcgcagattt taaataaaat attgtaatag catgaaagat
     2281 atagtagtta gtggtataag aagtacgggc aatttacatc ttggtaatta ctttggtgct
     2341 gttgcaaatt ttataaaaat gcaacaccag catccatgct ttttctttgt tgccgattat
     2401 cattcgctta ctacacatcc aaaccctgtt gatttattgg ctaatgaaag gcaggttata
     2461 atcgaatatc ttgcatgtgg tttagacccc gaaatatgta cactttattt gcaaagtgat
     2521 ataccacaaa cggctgaatt atatttgttc ttaaacatgc ttgcttattt aggcgagtta
     2581 gagcgtgtaa caacttttaa agacaaagcg cgcaaacacc ctgataatat aaatgccggc
     2641 ttacttacct atcctacatt aatggcagcc gatgttttaa ttcataaagg tgctaaagtg
     2701 cccgtgggca aagatcagga gcagcattta gaaatgatgc gcacttttgg aaatcgtttt
     2761 aaccgattgt ataatgttga ttattttccg gagccccagg cttttaattt cggtacggag
     2821 ttggttaaag tacccggttt agatggtagc ggtaaaatgg gcaaatcaga aggtgagggc
     2881 aatgcattgt ttttatgtga tgatgataaa accattcgca aaaaaattat gcgggctgta
     2941 acggatagcg ggcctacgca attgaatcaa cctaaaccgg atgccataaa aaatattttt
     3001 gctttaatga acctggtgtc ggctaaggat acggtaaact actttgatga acagtataac
     3061 aatatgacca tacgttatgg cgatttgaaa aagcaactgg ctgaagatat ggtgctgttt
     3121 attaatccgc ttgcacaaaa aattagcgac ctaaaacaga atgatgatta tgtaacaaaa
     3181 gtaatagcaa tgggtaagga gaaggctgcg gcaagtgcca ataaaactat taccgaagtg
     3241 cgtaagataa taggcttcaa gtaatttttt agcgcttaat ctgcaacaga cttccgcaac
     3301 ttttttttgt ggcttaatag ctttttatca tcactt
//
LOCUS       DAOMAA010000088         3329 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_21593_length_3329_cov_3.209835, whole genome shotgun sequence.
ACCESSION   DAOMAA010000088 DAOMAA010000000
VERSION     DAOMAA010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21593_length_3329_cov_3.209835"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="PLO59_01865"
     CDS             complement(<1..192)
                     /locus_tag="PLO59_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015027755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HQU99870.1"
                     /translation="MELEIFDKLGDKFFMRLLIDIISVTVLVRLVYYKIYRKKDFFFT
                     FFMFNIIIFIITYLLNKVEM"
     gene            complement(183..947)
                     /locus_tag="PLO59_01870"
     CDS             complement(183..947)
                     /locus_tag="PLO59_01870"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HQU99871.1"
                     /translation="METLQSAIDAFIPIDLKEMDAVALLDRTDTKYLFHISKLPLLLQ
                     GLQPLYRMLEVKGIRQNKYESHYFDDDNCTFYHMHHNKFGKRFKVRYRKYVDSAISFF
                     EIKKKINTNRTIKKRIPTQEIGCAVTGEEKAFFEKITHLNADVLHPTVRVDYTRLTLV
                     SNDLTERATFDLHIAFKNNGHTKSFDELVIVEVKQNKIDHSSPVIRQLKNMHITEASL
                     SKYCMGVYQLYDNVKKNNFKPQAHNLSKILKNKTWS"
     gene            complement(970..1383)
                     /locus_tag="PLO59_01875"
     CDS             complement(970..1383)
                     /locus_tag="PLO59_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99872.1"
                     /translation="MKNIITAALVLTAILWQSCEKEEGEGGTSSIKGTIITRDFNGSF
                     PVQDYSDYGAPEEDVYIIYGSEGNAIGDRVRTSFDGTFEFKYLRKGVYTIFVYSEDTN
                     LVAPVPSGKVVIKQTVEITKNKSTVTAPTITRVEL"
     gene            complement(1453..1914)
                     /locus_tag="PLO59_01880"
     CDS             complement(1453..1914)
                     /locus_tag="PLO59_01880"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HQU99873.1"
                     /translation="MKFLKIQVAVVLITAAFFSNACKSKKEMKSTYTTTSNTNQTIKL
                     NSFWVIAAFYENGNVSYPQTKLATISIANDLKSFTGETGCNSMRGPLTITGDSIKFGH
                     IAHTRRSCEHSATEQKVLQALQQVNRYEIKSAELTLFQNQTPIIQFESYRN"
     gene            complement(1943..>3329)
                     /locus_tag="PLO59_01885"
     CDS             complement(1943..>3329)
                     /locus_tag="PLO59_01885"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HQU99874.1"
                     /translation="KKGKMNLDVREGAKCVVYLNGAYWGVYDLRENPDDHDYTKLHYG
                     QDKFHLQYIKTWGSTWAQYGGGQALTDWTNFRNFIMNNNMADSANYKFVTDRLEVLSL
                     IDYVIVNTFTVCTDWLNYNTGWWRGMDSTGTKLKWSYILWDNDATFDFYINYTGLPST
                     LYTASPCDPLGLTGNSDPKGHITMLNKLRTNPDFDQLFITREIDVWNTVFSCDNMLSQ
                     FDSTRNVIDPEMTMQATKWGGTKAGWLANTNTMRNFISNRCNHLGTNGFANCFGVTGP
                     YDVIVTADPVNAGTIKFNTLELTQFPWQGKYYNGVATKVEAIADTNYVFNNWSSTNQT
                     FSPAPTASNATFSLTGNDSIIAHFTYSPTGIAMPTNIGIGLYPMPSTFNAQTKLVFEL
                     PDNMQVSLRIFNINGSEAISLINDHDKTMKGKYAVNVNFEKAKLSAGVYIAELIANGV
                     KKTTKLIYSPQ"
BASE COUNT          971 a          604 c          623 g         1131 t
ORIGIN      
        1 catttctact ttattaagca gataggtaat aataaaaata atgatgttaa acataaagaa
       61 ggtaaagaag aaatccttct ttctataaat tttataatac accaaacgaa ccagtaccgt
      121 tactgagatt atatcaatta aaaggcgcat aaaaaattta tcgcccagct tgtcaaatat
      181 ttctaactcc atgttttatt ctttaaaatt tttgaaaggt tgtgtgcttg cggtttaaag
      241 ttgttctttt ttacattgtc gtacagttga taaacaccca tgcaatattt actaagcgag
      301 gcttcggtta tatgcatgtt ttttaattgg cggattacag gcgaactgtg atcaatttta
      361 ttctgcttta cttctacgat taccaactca tcaaacgatt tggtatggcc attgttttta
      421 aatgctatgt gcaaatcaaa agttgcgcgc tcggttaaat cattacttac cagtgttaat
      481 ctggtatagt caacgcgtac ggttgggtgt aatacatcgg catttaagtg tgttatcttt
      541 tcgaaaaatg ctttctcttc tcctgtaact gcacaaccaa tttcttgcgt aggtatgcgt
      601 ttttttatgg tgcgattggt attaattttc tttttgattt cgaaaaaact gatagctgag
      661 tcaacatatt ttcggtagcg tactttaaat cttttgccaa atttattatg atgcatgtga
      721 taaaaagtac agttgtcgtc atcaaaataa tgcgactcat atttattttg cctgatacct
      781 tttacttcaa gcatgcgata caatggttgc aagccttgca acaaaagcgg taacttggaa
      841 atatgaaaaa ggtatttggt atccgtacga tccagtaatg ctacggcatc catttccttt
      901 aagtcaatgg gtataaaggc atcaatggca ctttgtaacg tttccataat attgagtaac
      961 aattatgaat tacaattcaa ctctggttat agttggagct gttactgttg acttgttttt
     1021 tgttatttca acggtttgct ttataacaac tttaccactg ggtaccggtg caaccaggtt
     1081 ggtatcttcg ctatacacaa aaatggtgta aacgcctttg cgtaaatatt taaattcgaa
     1141 agtaccgtca aaactggtac gcactctatc gcctattgca ttaccttctg atccataaat
     1201 tatgtacacg tcttcttcgg gggcgccata atcgctataa tcctgaaccg gaaatgagcc
     1261 gttaaaatcg cgggtaataa tggtgccttt tatggaagat gtgccgccct cgccttcttc
     1321 cttttcgcac gattgccata aaatagcagt taaaacaagg gctgctgtta taatattttt
     1381 cattgggagt atgtaaatga ttttattttt agaggattgt ttattcaatg tttgatgctg
     1441 attaaagttt ttctaattgc gataactttc gaattgtatg atgggtgttt gattttgaaa
     1501 caatgttagt tcagcacttt taatttcata acggtttact tgttgtaatg cttgaagtac
     1561 cttttgttct gtggcactgt gctcacatga cctgcgtgtg tgggcaatgt ggccaaactt
     1621 aattgagtcg ccagtaatgg ttaacggacc gcgcatacta ttacaacctg tttcgcctgt
     1681 aaacgatttt aaatcgttgg caatgctgat agtggctaat ttggtttgtg ggtaacttac
     1741 attaccgttt tcataaaatg ccgctattac ccaaaaactg ttcaacttaa ttgtttgatt
     1801 ggtgtttgaa gtagttgtat aagtcgattt catttctttt ttcgacttgc atgcattcga
     1861 aaaaaaagca gcggtaatta atactaccgc tacttgtatt tttaaaaatt tcaaaacggt
     1921 gtgttataaa aatcagaaat aattattgag gcgaataaat aagcttggta gtttttttaa
     1981 caccatttgc aattaactcg gcaatgtaaa caccggccga tagtttggct ttttcgaaat
     2041 ttacattaac agcatatttg cctttcatgg ttttatcatg gtcattaatt aaactaattg
     2101 cttcgctgcc attaatatta aatatgcgta aagatacctg catattatca ggcagttcga
     2161 aaaccagttt tgtttgtgca ttaaatgtac ttggcatggg atacaagcca atgccaatat
     2221 tagtgggcat agctataccg gttggcgagt atgtaaaatg tgcaataata ctgtcgttac
     2281 cagtaagcga aaaagtagca ttgcttgctg ttggtgccgg gctaaatgtt tgattggtcg
     2341 agctccagtt attaaataca taattggtgt cggcaatggc ttctacctta gtggccactc
     2401 cgttataata tttaccctgc catggaaatt gtgtaagttc caatgtatta aactttatag
     2461 tgcctgcatt tacagggtcg gcagtaacta taacgtcata agggcctgta acgccaaaac
     2521 aatttgcaaa accattggta cctaaatgat tgcaacggtt actgataaaa ttgcgcatgg
     2581 tattggtatt agctaaccag ccggctttgg taccacccca ttttgtggct tgcatagtca
     2641 tttcggggtc tattacattg cgtgtactgt caaactgcga cagcatatta tcgcaactaa
     2701 aaacggtgtt ccatacatca atttcacggg taataaatag ttgatcaaaa tcaggattgg
     2761 tgcgcaattt attaagcatg gtgatatgtc ccttcgggtc tgagtttccg gttaagccta
     2821 atggatcgca agggctggct gtataaagcg ttgatggcaa accggtgtag ttgatataaa
     2881 agtcgaatgt agcatcgtta tcccataaaa tataactcca ttttaatttg gtaccggtac
     2941 tatccatacc acgccaccat ccggtattgt aattaagcca atcggtacaa acggtaaacg
     3001 tattaacgat tacataatca atcagactca acacctctaa acgatcggta acaaatttgt
     3061 aattagccga atcggccata ttattattca taataaagtt tctgaagtta gtccaatcgg
     3121 ttaaagcctg accgccacca tattgagccc aggtgctacc ccatgtttta atatattgta
     3181 aatgaaattt atcctgccca taatgtagct tggtataatc atggtcatcc ggattttcgc
     3241 gtaaatcgta aacaccccag tatgcaccat ttaaataaac cacacacttc gcgccttcgc
     3301 gtacatctaa attcatcttt ccttttttg
//
LOCUS       DAOMAA010000089         3316 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_21745_length_3316_cov_2.658080, whole genome shotgun sequence.
ACCESSION   DAOMAA010000089 DAOMAA010000000
VERSION     DAOMAA010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21745_length_3316_cov_2.658080"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="PLO59_01890"
     CDS             complement(<1..471)
                     /locus_tag="PLO59_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99875.1"
                     /translation="MIALADKKPFYTACALFLVLLVYFSVKTFYWFYLLPVLLLVAMA
                     SLVYTRQLFFLLVFIVPLSINFDETPLGFGINIPTEPISFGLMLIAFFKIIMDGGIPK
                     RFLFHPLTILILLHLVWMLITTLTSQLPLVSFKFTLGRLCFVTVFFLYAVPLFSD"
     gene            complement(484..1452)
                     /locus_tag="PLO59_01895"
     CDS             complement(484..1452)
                     /locus_tag="PLO59_01895"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HQU99876.1"
                     /translation="MENEKYSSQLDFIGLLKIVLKWKKQLMVTAVIAAVAGFVITRPF
                     IMKPRFKSATVVFPSNIAPYATESATETMLQFLQSEEVRKLFIEKLNLYGHYNIDPKS
                     PLAYTYLSQRLSENMSISRTQYESIEIDCWDEDPKFAAAMCDTLIAANNIAISNLHRQ
                     KFNEVITLFEQKLALRKAEMDSAEIALQNIKLNYGIINFESQVKAISKVMYKNGGAQS
                     AFVKDQFKNLQEHGGQEFALTEHLFRLRGFYNDTKKDYQQAISDANKKLTYTTVVSKA
                     QVAEKKDTPKGTLIIMGLTASALLFALITILIIESYRKHTLKNSNA"
     gene            complement(1519..2115)
                     /gene="gldD"
                     /locus_tag="PLO59_01900"
     CDS             complement(1519..2115)
                     /gene="gldD"
                     /locus_tag="PLO59_01900"
                     /inference="COORDINATES: protein motif:HMM:TIGR03512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldD"
                     /protein_id="HQU99877.1"
                     /translation="MFNKKKIKAKIDHILFATSLLLFGCNNHYTPKPIGYFRIDLPEK
                     KYIPFNPAKCPFAFDVPAFTVIEADNRAKAEPCWYDINYPGLQGKINLTYLPIHNDVI
                     THIENCRTLAYKHAVKANGIDEVLITSNDGQSGGLMYLISGNAASSIQFFLTDSTTHF
                     LRGALYFNCPPQSDSLAPVIEFCKKDIEQLIHTLQWKQ"
     gene            complement(2108..>3316)
                     /gene="gldE"
                     /locus_tag="PLO59_01905"
     CDS             complement(2108..>3316)
                     /gene="gldE"
                     /locus_tag="PLO59_01905"
                     /inference="COORDINATES: protein motif:HMM:TIGR03520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated protein GldE"
                     /protein_id="HQU99878.1"
                     /translation="EVAYFSLTPAQLAQLKNQPGNRIHQLMLTLLNKPVRFIATLLIS
                     NSAVNIAIVLVSSYMMELVFNFEAYPLTGTIVNVVIITFVIVMFGDLIPKVYATKNNL
                     KLAERIAIPLYITHKLFYPFSYLLASTTSFIEKRMKKISYQVTIDDLTHAIDIASNTN
                     TPVEEKKILKGIVKFGNLDVTQIMKHRVDIEALDYNLPLSELLKLVVNYGYSRLPVYN
                     TSLDNVSGVLHIKDLLPHLNKNDDFEWQQLIRKPFFVPESKMINDLLDEFQSKKMHLA
                     LVVDEFGGVVGLVTLEDVLEEIVGELNDEFDEDETFYTRIDDNNFVFEAKTLLNDVSR
                     IMQIELTEFDGIAGETATLAGLVLELTGKIPLPGESVNFKNYKFTIESSDKRRIKRVK
                     ITRSVSPPYV"
BASE COUNT         1007 a          603 c          626 g         1080 t
ORIGIN      
        1 atctgaaaac aatggaactg cataaagaaa aaacactgtt acaaaacaca atcggcctaa
       61 cgtaaattta aaactgacta atggtaattg actggttagg gtagtaatta gcatccaaac
      121 caaatgcaat aaaataagta tggttaatgg atgaaataag aagcgtttgg gaataccgcc
      181 atccataata attttgaaaa atgctattag cattaaacca aatgatatag gttcggtagg
      241 gatgttaatg ccaaaaccta aaggggtttc gtcaaaattt atggatagcg gaacaataaa
      301 aaccagtaga aaaaacaact gcctagtata aactaaactt gccatggcaa ccagtaataa
      361 aaccggtaac aagtaaaacc aataaaatgt ttttacacta aaatatacaa gcaataccag
      421 aaacaatgca catgctgtat aaaaaggttt tttatcggct aaggctatca acgcaattct
      481 attttatgca ttcgaatttt taagcgtatg cttgcgatac gattcgataa tgagtatggt
      541 aattaaagca aacagtaggg cacttgcagt taatcccatt ataatgagtg tacctttggg
      601 tgtgtctttt ttttcggcta cctgtgcttt ggaaactact gtggtatagg ttagtttttt
      661 atttgcatcg cttatggctt gctgataatc ttttttagtg tcgttataaa atccgcgtaa
      721 gcgaaataaa tgttcggtta atgcaaactc ctggccacca tgttcttgta aatttttaaa
      781 ctgatctttt acaaaagcac tttgcgcacc gccattttta tacatcactt tgcttattgc
      841 tttaacctgt gattcaaaat taataatacc ataatttaat ttgatgtttt gcaaagcgat
      901 ttcagcacta tccatttctg ctttacgcaa tgctaatttc tgttcaaaca atgtaattac
      961 ttcattaaac ttctgacggt gtaaattgct tatagcaata ttattggcag ctattaaagt
     1021 atcgcacatg gctgcggcaa acttcgggtc ttcgtcccag caatcaattt caattgattc
     1081 gtattgcgta cggcttatgc tcatgttttc gcttaaacgt tgcgataagt aggtataggc
     1141 caaaggcgat ttgggatcga tgttatagtg gccgtaaagg tttagttttt cgataaatag
     1201 cttacgcact tcttcgctct gcaaaaactg aagcatagtt tcggtagcgc tttcggtggc
     1261 atacggtgca atgttgcttg gaaatacaac ggtggccgat ttgaaacgcg gtttcattat
     1321 aaacgggcgt gttattacaa aaccggccac ggcagcaatt acagccgtta ccattaactg
     1381 ttttttccac tttaaaacaa ttttaagtaa tccaataaag tcaagctggg agctgtattt
     1441 ttcgttttcc atcaaccggt tatttttttg aggcgccaaa aatagaaaaa gcctactatg
     1501 ctacaatgca gcatttgctt actgcttcca ttgcaaagta tgtataagtt gttcaatatc
     1561 ttttttgcaa aattcgatta ccggtgccaa tgaatcgctt tgtggagggc agttaaaata
     1621 taatgcacct cgtaaaaagt gtgtggtact atcggttaaa aaaaactgaa tgctgctggc
     1681 agcattgccg cttataagat acatcaatcc gcccgactgg ccatcgttgc tggttataag
     1741 cacttcgtca atgccattag cttttacagc atgtttgtag gctaatgtgc ggcagttttc
     1801 tatatgcgta atcacatcat tatgtatggg caaataagtt aggtttattt tgccttgcaa
     1861 accagggtaa ttgatgtcgt accagcaggg ttcggctttt gcacggttgt cggcttcaat
     1921 tactgtaaag gcgggcacat cgaaggcaaa cgggcatttg gccgggttaa atgggatgta
     1981 ttttttttcg ggcaagtcaa ttcgaaagta acctattggc ttgggtgtgt aatggttgtt
     2041 acacccaaat aataataggc ttgtagcaaa aagtatatga tcaatttttg ccttaatttt
     2101 ttttttgtta aacatatggg ggcgaaaccg agcgtgtaat ttttacgcgt ttaattctgc
     2161 gtttatccga tgattcgatg gtaaacttat agtttttgaa atttactgat tcgcccggca
     2221 gtggaatttt accggttagc tcaagcacta atcctgctaa tgtggcggtt tcgcctgcaa
     2281 taccatcaaa ctcagttaat tctatttgca taatacggct aacgtcattt agtaatgttt
     2341 tagcttcgaa aacaaagtta ttatcatcta tacgggtgta aaaggtttcg tcctcatcaa
     2401 attcatcatt aagctcgccc actatctctt ctaaaacatc ttctaatgtt accaaaccaa
     2461 caacacctcc aaattcatca acaaccaatg ccaagtgcat ttttttcgat tgaaactcat
     2521 ccaacaaatc atttatcatt ttgctttcgg gtacaaaaaa tggtttgcgt ataagttgct
     2581 gccattcaaa atcatcgttt ttattaagat ggggcaataa atcttttata tgtaatacgc
     2641 cacttacatt gtctaaactt gtgttatata ccggcaagcg tgagtagcca tagtttacta
     2701 ccaatttaag caattcggat aaaggcaggt tgtaatcaag tgcttcaata tcaacccggt
     2761 gtttcataat ttgggttaca tctaagttgc caaactttac aatgcctttt aatatttttt
     2821 tttcttctac cggtgtgttg gtgttactgg ctatgtctat ggcatgtgtt aagtcgtcaa
     2881 ttgtaacttg gtaacttatt tttttcatac gcttttcaat aaagctggta gtacttgcca
     2941 gcaaatagct aaacgggtaa aaaagcttat gcgttatgta caacggtatg gctatacgct
     3001 cggccaactt taaattgttt ttggttgcat atactttcgg tattaaatcg ccaaacatca
     3061 caatcacaaa agtgattatt accacattaa caatggtacc ggttaaagga tacgcttcga
     3121 aattaaacac aagctccatc atgtatgatg ataccagcac aatggcaata tttacagcac
     3181 tgttcgaaat aagcaacgta gctataaacc gcacaggttt atttaaaagt gtaagcatta
     3241 gctgatgaat gcgattgccg ggctgatttt ttaattgtgc taactgagcc ggtgttaacg
     3301 aaaagtatgc tacttc
//
LOCUS       DAOMAA010000090         3310 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_21819_length_3310_cov_2.602151, whole genome shotgun sequence.
ACCESSION   DAOMAA010000090 DAOMAA010000000
VERSION     DAOMAA010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21819_length_3310_cov_2.602151"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1661)
                     /locus_tag="PLO59_01910"
     CDS             complement(<1..1661)
                     /locus_tag="PLO59_01910"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HQU99879.1"
                     /translation="MKKFTHSLAKLKLIATAIALLITAQLQAQVTNVNTASTYGTLGA
                     AISDGSTLAGHTLILTANLTEGLVTVNKSLTIDGQGFTLTSTSLTYGINPVTTNVSIQ
                     NLTVTGSGTFGILAPCGSHNLNLTNVTVTACGGSGIAISGSDNCVFTNVTCTSNNGNG
                     ISITNCDNTSIVGYTSSGNSFGGGFGAGIGLFTSSGFCLPAGINGFSLTGTVTIAENP
                     RVYSQKAATAHVITGITGASIQWAVGTSANDRSYWPSKPIAYSVVDALFEPPYSFPNT
                     TVYVEEVATDNLYVDDNPNGDATPPMLINTAVAMAQAGKTIFLEAGTFAERVIVDKSL
                     TIDGDGMTTSILDGTGLPGTGSGISINNNITNCTVQELTVRNYALGSSAHAGIAAQLS
                     NNNLTVYKCDVYNNSGGRGGVYVNGPVNNVLIDSVKAHDHPAGSRGIVVWNGLKQNVT
                     IQNCEVYNNNCCGIELQDGTASGINVLNNNVYNNADNGIGLNGVKGGTGANVVTGNTV
                     SNNGRFGIEVKNANADGTNAGVNSFVISGNTVSLTAQGNAQSYSW"
     gene            complement(2016..2372)
                     /locus_tag="PLO59_01915"
     CDS             complement(2016..2372)
                     /locus_tag="PLO59_01915"
                     /inference="COORDINATES: protein motif:HMM:NF016056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HQU99880.1"
                     /translation="MIVIYDGNCMFCSRLVFWLIRNNKDEMMRFAAAQSTTAIKYATE
                     MGFVNQLGKSILVVLGNKYYTQAEAIFVTATKLKLPFSIIGFLKILPTTFTNWCYRLI
                     ARNRHHLAKHKCNFNS"
     gene            complement(2374..>3310)
                     /locus_tag="PLO59_01920"
     CDS             complement(2374..>3310)
                     /locus_tag="PLO59_01920"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HQU99881.1"
                     /translation="TSIQPFATKACILKTKNQMQGIVLKGVDADYHWHYLKQQIVKGT
                     IPAFTPGQASTDIIISSTVAKLLQLDTGKNVFSFFIDGNMQRIKKCKVVGIYDTGFEE
                     VDKTFVICDLNLIRQLNHWKNQEIGGYEIMLDDYKNLETTTNDIYNISGADFNTKTID
                     EAYPQIVSWLNLIDTNVYIIIGLMCVVACINMISALLIIILDQTKTIGLYKALGATDK
                     SIRKIFLIVAAYLLFYGLLIGNALGFALAIGQYYFKWFALDKATYYLDAVPVSFNWLQ
                     LVCLNLATALICFVFLLLPSKLISKLMPVKAIKFV"
BASE COUNT         1050 a          639 c          592 g         1029 t
ORIGIN      
        1 tcccagctgt aactttgtgc attgccttgt gccgttaaac taacggtatt gccactaata
       61 acaaagctgt ttacaccggc attggttcca tcggcattgg cattttttac ttcgatacca
      121 aaacggccgt tgttactaac ggtgttaccg gtaacaacat ttgcacctgt accacccttt
      181 acaccattta agcctatacc attgtcggca ttgttataaa cattgttgtt taatacattt
      241 attcccgatg ctgtaccgtc ttgcaactca ataccacagc agttattatt atatacttcg
      301 caattttgta tggtaacatt ttgctttaag ccattccaaa ctactatacc acgcgaaccg
      361 gcaggatggt cgtgtgcctt tacgctatca ataagtacat tattaacagg gccgtttaca
      421 tatacaccgc cacggcctcc gctattatta tatacatcgc atttataaac cgttaagttg
      481 ttattgctta gctgtgcagc aataccagca tgtgctgaac tacccagtgc ataatttctt
      541 acggtaagct cctgtacggt acaattggta atattattat taatagatat accactacct
      601 gtgccgggca aaccggtacc atctaaaata cttgtggtca ttccgtcacc atcaatagtt
      661 aatgatttat ctacaatcac cctttcggca aaagtgcctg cttctaaaaa tatggtttta
      721 ccggcttgag ccatggccac tgctgtatta ataagcatgg gaggtgttgc atcgccattt
      781 ggattatcgt caacgtataa attatcggta gcaacttctt caacataaac cgttgtattt
      841 gggaaactgt aaggtggttc gaataaggca tctacgactg aatatgcaat aggttttgaa
      901 ggccagtaac ttctgtcgtt ggcactggta ccaacagccc attgaatgga agcgcccgta
      961 attcctgtaa taacatgtgc tgtagccgct ttttgagaat atacgcgcgg attttcggca
     1021 atggttaccg taccggttaa gctaaagcca tttatacctg ccggtaaaca aaagcccgaa
     1081 ctggtaaaca gtccgatacc cgcaccaaaa cctccaccaa atgaattgcc acttgaagtg
     1141 taccctacaa tggaggtatt atcacaattg gttattgaaa taccgttacc gttattgctg
     1201 gtacaggtaa cgttagtaaa tacgcagtta tcagacccgg aaatagcaat tccggaacca
     1261 ccacaagccg taactgtaac attggtaagg tttaagttgt gagaaccgca tggcgctaaa
     1321 attccaaagg tacctgaacc tgtaactgta agattttgga tactcacatt ggtggtaacc
     1381 ggatttatac cataagttag cgaagttgag gttaaggtaa atccttgccc atcaatagtt
     1441 aacgacttat ttacagtaac caaaccttcg gtgagattag ctgttaatat taaagtatgg
     1501 ccggctaaag ttgatccatc actaatagca gcacctaaag taccataagt acttgcagta
     1561 tttacgttgg taacctgcgc ctgcaattga gctgttatta gcaaggcaat agctgtggct
     1621 attaatttta gtttggctaa ggaatgggta aattttttca taggaaataa attttatcgt
     1681 ggcgtgttaa ggaggttaaa cattgatgtt actgctaact acaacaaagt gttgcagatg
     1741 aaataggctg aatgaaagct ttaaaaacaa taacttgcga atccagtaag tatggggggc
     1801 ggggtaaatc aaggcaggaa gaatgaatac taaaagtaag ggggttatta ggtttcggag
     1861 ttaaaattta aactaccagg gtaataattc aactgccaaa aatataaagt taattaatac
     1921 ggcaaaattt aattttgccg taagtcacct ttcaaactgt caggatagaa tttttattaa
     1981 caaacttatt aaggcaaaaa acgatgttta taaatttagg aattaaaatt gcatttgtgc
     2041 ttggctaaat gatgtcggtt tcgagctatc aaccggtaac accaatttgt aaatgttgtt
     2101 ggcaaaattt ttaagaaacc aattatagaa aatggcaatt ttaatttagt ggcggtaaca
     2161 aaaattgctt cagcttgtgt atagtatttg ttgcccaaaa caaccagaat gcttttacct
     2221 aactggttta caaatcccat ttcagttgca tattttattg ccgtggtact ttgggctgca
     2281 gcaaagcgca tcatttcatc tttgttgttt ctaattaacc aaaaaaccaa tcggctacaa
     2341 aacatacaat ttccatcata aataactatc atactaaaca aacttaatgg ctttaaccgg
     2401 cataagttta ctgattaatt tactgggtaa gagcagaaaa acaaaacaaa taagtgctgt
     2461 ggctaaattt aaacacacta attgcaacca attaaaactt acaggcacag catccagata
     2521 atacgttgct ttatccagtg caaaccattt aaaataatat tgaccaatag ctaatgcaaa
     2581 tccaagagca ttgccaatta aaaggccgta aaacaataaa taggcggcaa caattaaaaa
     2641 gatttttcgg atgcttttat cggttgcgcc taaagcttta tacagaccaa tggtttttgt
     2701 ttggtctaaa attataatca gcaatgccga aatcatatta atacaagcta ctacacacat
     2761 taaaccaatt ataatataaa cattggtatc aattaagttt agccagctca cgatttgcgg
     2821 ataagcttca tctatagttt ttgtattaaa atcagcccca gagatattat atatgtcatt
     2881 tgttgttgtt tccaggtttt tataatcatc caacataatt tcataaccgc caatttcctg
     2941 atttttccaa tggtttaact gcctaattaa gtttaaatca caaatcacaa aagttttgtc
     3001 aacttcttca aagcctgtat cataaatccc cacaaccttg cactttttaa tacgctgcat
     3061 attgccatca ataaaaaaac taaatacatt tttgcctgtg tcgagctgaa gtaatttagc
     3121 aactgttgat gagattatta tatcagtact tgcttgcccc ggtgtaaagg ctggtattgt
     3181 gccttttact atttgctgtt taagataatg ccaatgataa tctgcatcaa cccctttaag
     3241 cacaattccc tgcatttggt tttttgtttt cagtatgcat gctttggttg caaaaggctg
     3301 tatagaggtt
//
LOCUS       DAOMAA010000091         3303 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_21900_length_3303_cov_2.675185, whole genome shotgun sequence.
ACCESSION   DAOMAA010000091 DAOMAA010000000
VERSION     DAOMAA010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21900_length_3303_cov_2.675185"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..152
                     /gene="rpmF"
                     /locus_tag="PLO59_01925"
     CDS             <1..152
                     /gene="rpmF"
                     /locus_tag="PLO59_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013620844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HQU99882.1"
                     /translation="RRDKRRTHYKATAPTVMVDKTTGESHLMHRAYWLENKLYYKGKV
                     VMERA"
     gene            241..1200
                     /gene="plsX"
                     /locus_tag="PLO59_01930"
     CDS             241..1200
                     /gene="plsX"
                     /locus_tag="PLO59_01930"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017256837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HQU99883.1"
                     /translation="MRIGLDIMGGDYAPLEITLGALLAQPLLTEVRLVLIGSESAARK
                     IITENQGNPANFDFVNSIDVIEMHEHPAKAFQQKPNSSISIGFHLLKEGKIDAFCSAG
                     NTGAMLVGSMFSVKTIQGIMRPAISTLVPKEKGGYGLLLDVGVNADCKPENLQQFAML
                     GSLYAEHVCHIPNPKVALMSIGEEDEKGNVLTQAANQLLKNTSGINFVGNVEGRDLFN
                     DKADVIVCDGFTGNIMLKLAESFYSLTTKRKLKDEYFDKFNFESYGGTPILGVNGTVM
                     IAHGISNATAVKAMLVLTHEVVAAQLPQKIASAIASQVPQANQ"
     gene            1291..1818
                     /locus_tag="PLO59_01935"
     CDS             1291..1818
                     /locus_tag="PLO59_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HQU99884.1"
                     /translation="MDEQQLVTNCLKGDKTAQKQFYEMFARKMMGVCLRYSNDLATAE
                     DYLQESFIKVFGKLSQYQFKGSLEGWVKRIVVNVVLDELRKNRILFDSNAEVTDVLQT
                     TDDVVSNMEAKQLLQIIQRLPTGYRTVFNLYAIEGYQHNEIANMLQISESTSKSQYQK
                     AKIQIQKTLKAQTII"
     gene            1842..2687
                     /locus_tag="PLO59_01940"
     CDS             1842..2687
                     /locus_tag="PLO59_01940"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HQU99885.1"
                     /translation="MFKDAFENFEAEVNPQIWVQVQQQIPTTAVTPQASVTFSGVKTL
                     LIAGVTAIAAITAAVVYKSEPKLNTLVESKDNPVDNKVEKTVAPVQSIEKVLPELIKE
                     NETQKVQPAPAPASTTVLKLPETTNESSANNDNQASEPLNTVTNQTKIKESEPVVEVI
                     NQDDKHENNLSSIQNTEAEKTKETEEIKDFNWGRISNTFTPNADGKNDVFKIHLPDAN
                     YFKATILNINGKILHEWEGENGFWDGKDAGGYDLPKGMYVYYIIATDTNGNTHKTKGS
                     ISILK"
     gene            2715..>3303
                     /locus_tag="PLO59_01945"
     CDS             2715..>3303
                     /locus_tag="PLO59_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99886.1"
                     /translation="MKKLFTLLIAITAFVVLGLQSAEASHAQGADITYTYTGTPNEYI
                     ITLRFYRDCAGIDAPPSVDICYSSASSGFSSTIIMFPIAGTGNPIPSSSCVTTPTNCT
                     GFGGFGTEEWIYQEVLTLPNAANDWIFSYNVCCRNTQITTLANAGGQDIYVSATLNNL
                     DFPTNSSPYFDSVPVTTFCVNNQFYYYQGAFDVDGD"
BASE COUNT         1146 a          621 c          629 g          907 t
ORIGIN      
        1 caagacgtga taagcgcaga acacattaca aagcaacagc cccaaccgtt atggttgata
       61 aaacaactgg cgagtcgcat ttaatgcacc gtgcctactg gttagaaaac aagctctact
      121 ataagggcaa agtagttatg gaacgcgctt aaattcaata aaacctgtac gatgattttt
      181 tttgggtaca ggttttttcc tttttttata atttcaaagt tctaagctta gtatttaagt
      241 atgcgtatag gattggacat catgggtggc gattatgcac cgttagaaat aactttaggt
      301 gcacttttag cacaaccgct tttaaccgaa gtccgtttgg tattaatcgg aagtgaatcg
      361 gcagcccgta aaataattac ggaaaaccaa ggcaatccgg ctaacttcga ctttgttaac
      421 agtatcgatg taattgaaat gcacgaacat ccggccaaag catttcaaca aaaaccaaac
      481 tcaagcatta gtatcggatt tcacttactt aaagaaggta aaattgatgc cttttgcagt
      541 gccggaaata ccggagccat gttagtgggc agtatgtttt cagtaaaaac aatacaaggc
      601 attatgcgac ccgctataag tacactggtt ccaaaagaaa agggcgggta tggtttgctt
      661 ttagatgtag gcgtaaatgc cgactgcaaa cccgaaaacc tgcaacaatt tgctatgtta
      721 ggctcacttt atgccgaaca tgtttgccat atacctaacc caaaggtagc tttaatgagt
      781 attggcgagg aagacgaaaa aggcaatgtg cttacacaag cagcaaacca attattaaaa
      841 aatacttcag gaattaattt tgttggaaac gtagaaggtc gcgacctgtt taatgataag
      901 gccgatgtta ttgtttgcga tggttttaca ggcaatatca tgctaaaatt agccgaatca
      961 ttttattcgc ttactacgaa acgcaaactt aaagacgagt attttgataa gtttaatttc
     1021 gaaagctacg gaggaacacc cattttaggc gtaaatggaa ccgtaatgat agcacatggt
     1081 atatcaaatg ccacagctgt taaagccatg cttgtgttaa cgcatgaagt tgtagcagcg
     1141 caacttccac aaaaaatcgc ctcagcaatt gcctcgcaag tgccacaagc caaccaataa
     1201 cttgccggta cagttttttt aaattttttt tataacccag gcgtcttttc attaaataat
     1261 tacgtcttaa ggttaattca aacatttgta atggatgagc aacaactcgt aaccaactgt
     1321 ttaaaaggag acaaaacagc tcaaaagcag ttttacgaaa tgtttgcccg caaaatgatg
     1381 ggagtttgtt tacgctactc caacgatttg gcaacagccg aagattattt gcaggaatca
     1441 tttattaaag tgtttggaaa gttaagtcaa tatcagttta aaggttcttt agaaggctgg
     1501 gtaaaacgaa tagtagtaaa tgttgtttta gatgaactcc gtaaaaaccg gattttgttc
     1561 gatagtaatg ccgaagtaac ggacgtactg caaacaacag atgatgtagt ttcgaacatg
     1621 gaggcaaaac aattattgca gataatacaa aggttgccca ccggttacag aaccgtattt
     1681 aacttatatg ctatcgaagg ttaccagcat aacgaaattg ccaacatgtt acaaattagc
     1741 gagagcacat caaaatcaca ataccaaaaa gcaaaaatcc agattcagaa aacactaaaa
     1801 gcacaaacta ttatttaatg caacccacct cgtttgaaaa tatgtttaaa gatgcattcg
     1861 aaaatttcga agcagaggtt aatccacaaa tttgggtgca agtgcaacag caaattccaa
     1921 ccactgcagt tacaccacaa gcaagtgtta ctttttcagg ggttaaaaca ttattaattg
     1981 caggtgtaac agccattgct gcaattacag cagcggttgt ttacaaatcc gagccaaagt
     2041 taaataccct ggttgaaagc aaagataatc ccgttgataa taaggttgaa aagactgtag
     2101 cgcctgtaca aagtattgaa aaggtattac cggagcttat aaaagaaaac gaaacccaaa
     2161 aagtacaacc cgcgccagct cctgcttcaa caaccgtttt aaaattgccc gaaacaacaa
     2221 atgaaagttc ggcaaacaat gataaccaag cttccgaacc attaaacacc gtaaccaatc
     2281 aaactaaaat aaaagagtca gaaccggtag tcgaagttat aaatcaggat gataagcatg
     2341 aaaacaacct atcatcaatt caaaacacag aagctgagaa aacaaaagaa actgaagaaa
     2401 ttaaagactt taattggggc agaataagca atacttttac acccaatgcc gatggaaaaa
     2461 atgacgtttt taaaatacat ttacccgatg ccaattattt taaagcaacc attttaaaca
     2521 taaacggaaa aattttgcat gaatgggaag gcgaaaacgg cttttgggat ggaaaagatg
     2581 ctgggggtta tgatttacca aagggaatgt atgtgtatta cattattgca actgatacaa
     2641 acggaaatac acataaaaca aaaggaagca ttagcatatt aaaataaata ttaaacaatt
     2701 ataattatat caccatgaaa aaattattta cattattaat tgcaatcaca gcctttgtag
     2761 tgttgggttt gcaaagtgcc gaagcatcac atgcacaagg tgccgacatt acctatactt
     2821 ataccggtac gcccaatgag tatattataa ctttgcgttt ttaccgcgat tgtgccggta
     2881 ttgacgcacc accatctgta gatatttgtt actcatcggc atcttcaggt tttagtagta
     2941 ccattattat gtttcctatt gcaggtacag gtaatcccat tccaagctca tcatgcgtaa
     3001 ctacgcctac caattgtaca ggctttggtg gttttggtac tgaagaatgg atttaccagg
     3061 aagttttaac cttgcctaat gcggcaaacg actggatttt cagctacaat gtttgttgcc
     3121 gtaatacgca aattacgacc ttagccaatg cgggcggaca ggatatttat gtaagtgcca
     3181 ccttaaataa tttagatttc ccaaccaaca gttcacctta ttttgactcc gtaccggtta
     3241 ctaccttttg tgtaaataac caattctatt attaccaggg agcttttgat gttgatggcg
     3301 att
//
LOCUS       DAOMAA010000092         3294 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_22002_length_3294_cov_2.863229, whole genome shotgun sequence.
ACCESSION   DAOMAA010000092 DAOMAA010000000
VERSION     DAOMAA010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22002_length_3294_cov_2.863229"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="PLO59_01950"
     CDS             complement(<1..223)
                     /locus_tag="PLO59_01950"
                     /inference="COORDINATES: protein motif:HMM:NF024807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HQU99887.1"
                     /translation="MWVQKASLPGLARYEAAAFTLNGKGYFISGGDYNTGNIFNDVWE
                     YDPVNDLWTQKASLPGLARYGASAFSVGNK"
     gene            complement(379..1182)
                     /locus_tag="PLO59_01955"
     CDS             complement(379..1182)
                     /locus_tag="PLO59_01955"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQU99888.1"
                     /translation="MEKIVDKAGADYWTQVWQNTSLPLPLNTSETLSNTITLRFQEVF
                     RKFFDTVTTNDKLLLEVGCGNSVWLPYLAKQYGFKIYGLDYSNYGCETERAILKRDFV
                     EGEIIIGDMFNPPVNLLNKFDFVISMGVIEHFSDTTQVVKSLSSFLKPDGVLITTLPN
                     HAGILGWLQKVFNKPVYDIHFILDKTDIENSVANADLKMIQSLYAVSGGFMVNLEAKD
                     KPLPNIFVRKIGAKVLVVLTYLWWSINKYIVKLPENKFLSPAIINIAKK"
     gene            complement(1191..2132)
                     /locus_tag="PLO59_01960"
     CDS             complement(1191..2132)
                     /locus_tag="PLO59_01960"
                     /inference="COORDINATES: protein motif:HMM:NF019615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQU99889.1"
                     /translation="MLQQFISPVTGNPLQEQNGVLTNHSGEKFPVIHAIPRFVPSDNY
                     ASAFGLQWKSFAKIQLDSYNGSTISYDRLLRCFGNNLQQIAQKKLLEVGCGAGRFTEH
                     LIKAGAYVHSVDLSEAVEVNYSNMQGAANYQVAQANVYNLPYAPQSFDVVMCLGVIQH
                     TPNPEKTIEALWNMVKPGGLLVIDHYTFDWMYYLKPILLYRAFLRKMKPERSKKIVER
                     LVRFFFPLHWKYRNSKIATVLLNRISPCYVYIKTFPQMDHDFHYELTRLDTYDGLTDY
                     YKHLRSRKQIENKLKALDAKNIKVWKGGNGVEAMCNK"
     gene            complement(2321..>3294)
                     /locus_tag="PLO59_01965"
     CDS             complement(2321..>3294)
                     /locus_tag="PLO59_01965"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU99890.1"
                     /translation="GATGYTWTATSGATVSSGQGSTNVVINFSNLATNATLSVVATNL
                     CGSSIARTLSYTVATEAPAKPATITGMLFGLCNKPSVPYSCPTVVGATSYLWSVPTGV
                     TILSGQGTNSITVQYTGSFTGSGTLSVSAQNGCGSSALTSRIVNAKPQQPVISGQNWA
                     CKGQAGLTYTVSAVAGATSYQWIPVPGSTITSGQGTTSITYKWGNIHGVVKCIAKNAC
                     GNSIAGNLSVSFTCKTTDTDENFSDITLYPNPASETATLSFESFIEGEAAVYIRDMIG
                     QELLNDNVKVEKGNNNYILRLSEFPKGVYLVSLKFKGISQNSRLIIQ"
BASE COUNT          969 a          626 c          615 g         1084 t
ORIGIN      
        1 ctttattgcc tactgaaaat gcagatgcac cataacgtgc caaaccaggt aagcttgcct
       61 tttgcgtcca aagatcattt acagggtcat actcccaaac gtcattaaaa atatttcccg
      121 tattgtaatc acccccggaa ataaagtagc ctttaccatt aagtgtgaat gctgctgctt
      181 cgtatcttgc caatccgggc aaagatgctt tttgtaccca cacgttaggc tgcgaaatta
      241 aataaacgtt cttgcaaact aatattaaag taaagatcga ataaagatac agacgcgata
      301 aaaccatttt caaaagattt tacttgacat tgtgtttaag aaatggaggg gttaaatttt
      361 taattaaaaa ctttgcattt atttcttcgc aatatttatt atggctggtg acaagaattt
      421 gttttcgggc aatttcacaa tgtatttatt tattgaccac cataagtatg taagaacaac
      481 cagaacttta gcaccaattt ttcgtacaaa tatattgggc aaaggtttgt cttttgcttc
      541 gaggttaacc ataaaaccgc cactaactgc atatagactt tgaatcattt ttaaatctgc
      601 attcgcaaca gaattttcaa tatcagtttt gtctaaaatg aaatgaatat cataaacagg
      661 tttattaaaa accttttgaa gccaacctaa gatgccggca tgattaggca atgttgtaat
      721 taatacgcca tcgggtttta aaaaacttga taatgatttt acaacttgtg ttgtatcgga
      781 aaaatgttcg ataacgccca ttgagataac gaagtcaaat ttgtttaaga gattaacagg
      841 tggattaaac atgtcgccaa taattatttc tccctcaaca aaatcacgtt tcagaattgc
      901 tctttcggtt tcacaaccgt agtttgaata atcaagacca taaattttaa aaccatattg
      961 ttttgcaaga taaggtagcc ataccgaatt gccgcatcct acttcaagca acagcttgtc
     1021 atttgttgtt acggtatcaa agaatttcct gaatacttcc tgaaatctta atgttattgt
     1081 attagataag gtttctgaag tatttaatgg taggggcaaa gaggtgtttt gccaaacttg
     1141 agtccaatag tcagcacctg ctttatcaac tattttttcc attctatttt ttatttatta
     1201 cacattgctt caacgccatt gccacctttc cacactttaa tattttttgc gtccagcgct
     1261 tttaatttat tttcaatttg ttttcgacta cgcaaatgtt tgtagtaatc agttaatcca
     1321 tcataggtat ctaaacgggt taattcgtaa tgaaaatcgt gatccatttg cggaaacgtt
     1381 tttatataca cataacaagg cgatatacgg ttaagcaaca cggttgctat ttttgagttt
     1441 ctgtatttcc aatgtaaagg aaagaaaaag cgtaccagac gttctacaat ttttttcgag
     1501 cgttcaggtt tcatctttcg taaaaaagca cgataaagca atatgggttt caggtaatac
     1561 atccaatcaa aagtatagtg gtcaattacc aaaaggccac cgggttttac catattccaa
     1621 agtgcttcaa ttgttttttc ggggttaggg gtatgttgta ttacgcccaa acacattaca
     1681 acatcaaaac tttgtggtgc ataaggcagg ttatatacgt tggcttgtgc aacctgataa
     1741 ttggctgcac cctgcatatt gctgtagttt acttctacgg cttcgcttaa gtcaacacta
     1801 tgtacatagg cgcctgcttt aattaaatgt tcggtaaaac ggccggcacc acagcctact
     1861 tccaataatt ttttttgcgc tatttgttgt aaattatttc cgaagcaacg taacaagcgg
     1921 tcgtaactaa tggtactgcc attgtaacta tcgagctgaa ttttggcaaa cgatttccat
     1981 tgtaaaccaa aagcacttgc ataattatcg ctgggtacaa agcggggtat ggcatgtatt
     2041 accggaaatt tttcgcctga atggtttgtt aatacaccat tttgttcttg taaaggattg
     2101 cctgtaacag gtgatatgaa ttgctgcagc atgtaatgca tgtaatgata aacgtgcaaa
     2161 aatatttatt tgtttcaatt atggttttga cactaccaca ttattgtagc aacacttttt
     2221 gctgaaagtt tacttatatg tattttatct gaacgtagcg taaaaagccg aaagggtagc
     2281 acatgctacc ctttcggctt gatgacggaa acgtttttgt ttattgtatt ataagcctgc
     2341 tgttttggga aatgccctta aatttaaggc ttactaaata tacgccttta gggaattcgg
     2401 atagacgtag tatataatta ttatttcctt tttccacttt cacgttgtcg tttaataatt
     2461 cttgtccaat catgtccctg atataaactg cagcttcccc ttcaataaag ctttcaaatg
     2521 ataacgtagc agtttcagat gccgggtttg gataaagtgt tatatcggaa aagttttcgt
     2581 ctgtgtcagt cgttttacac gtaaatgaaa ctgataaatt acctgcaata ctgtttccac
     2641 aggcattttt agcgatacac tttacaacgc catgaatatt accccatttg tatgtaatag
     2701 atgttgtacc ctggccactt gtaatggtac tgccgggcac gggtatccac tgatagcttg
     2761 tagcacccgc aactgcggat acggtgtacg ttagtcctgc ctgccctttg catgcccagt
     2821 tttgtcctga aattacaggc tgttggggct ttgcattaac tatgcgtgac gtaagtgcac
     2881 tactaccaca accattttga gctgatacac ttaatgttcc tgagcctgta aatgatccgg
     2941 tgtattgaac ggtgatggag ttggtacctt gccctgaaag tattgtaacc ccggtaggta
     3001 ctgaccataa ataagatgtg gctcctacca ccgtaggaca cgaatatggc acactgggct
     3061 tattacatag gccgaacaac atgccagtaa tggttgcagg tttagcaggt gcttcggtag
     3121 ctactgtata tgataatgta cgtgcaatac ttgaaccgca taaattagtt gctacaacag
     3181 aaagtgtggc attagttgcc aaattgctga aattaattac cacattagtt gagccttgtc
     3241 cgctacttac tgttgcacct gaagtggctg tccatgtgta gccggttgcc cctg
//
LOCUS       DAOMAA010000093         3271 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_22282_length_3271_cov_2.783271, whole genome shotgun sequence.
ACCESSION   DAOMAA010000093 DAOMAA010000000
VERSION     DAOMAA010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22282_length_3271_cov_2.783271"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="PLO59_01970"
     CDS             complement(<1..411)
                     /locus_tag="PLO59_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99891.1"
                     /translation="MKKITYIFLLTLININVYSQVDVLNHTNNSATWYVGWDNSAQSN
                     LYNLNIEHRGTQNINFLTGTPSVNRMTILNGGNVGIGVANPTSRLDVLNDINIQVNAI
                     NNAYRLNGVTVLQNWGSRNIFTGAGAGAALTLGAD"
     gene            complement(408..1367)
                     /locus_tag="PLO59_01975"
     CDS             complement(408..1367)
                     /locus_tag="PLO59_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU99892.1"
                     /translation="MKKLLIIFLMLLARICSAQLNLVPNPSFEDTVSCPTSAFEISKA
                     DNWYSCRSTPDYFNICCNLYSGIVGVPNNVLGYQFPRTGNAYAGLIPYDINGLLGWEY
                     IGTTLLTPTIIGQKYFVTFYISLADNTLFAINKFGIMLTMTPYSFNNPYPVNNSSLIY
                     SDSIIIDTTGWSKIQLSFVADSNYQYLMLGNFFDYSLTDTVGSNRTFAYYYFDDICMS
                     TDSVYCDNYTGLNHSTSNQPQTIIKNSYLKDDKLKVIFNDNYIEKEILIYSVSGQLLN
                     TTKTMDQQIDIMLPGSSTIYFLCVYEKSKMESKKIIAIKNYIE"
     gene            1989..2486
                     /locus_tag="PLO59_01980"
     CDS             1989..2486
                     /locus_tag="PLO59_01980"
                     /inference="COORDINATES: protein motif:HMM:NF025749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4421 family protein"
                     /protein_id="HQU99893.1"
                     /translation="MKKIILLFFLINSVALKAQHDSTYFKKYSHKLALGFFSTLRNYN
                     LRINSNISKDAGLPDVDFATNGRLQWGLAFGYDKIRLGIAIGKSANNDIYGQSRTQNI
                     AADIGGNAIFIAVGYRRYTGMYDKNSTGYMPANGPTFYIDPSCIFSIIKNMLLALHTW
                     QISDK"
     gene            2465..2980
                     /locus_tag="PLO59_01985"
     CDS             2465..2980
                     /locus_tag="PLO59_01985"
                     /inference="COORDINATES: protein motif:HMM:NF025749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4421 family protein"
                     /protein_id="HQU99894.1"
                     /translation="MANQRQIKSAASWLVTSNIHHEKIKTAATIIPPPLASYYGGDAQ
                     LQTLQWWGINMGAGATGNLVILRKLFIHGTISFGAETQFREYVYNTGYNKKVTAMGFA
                     GDFKAGIGFNSESFFISINNNIDYHSVSLSNLKLVESLVTGYLIIGYRFKVKDPPLYQ
                     KLRETRLYKSL"
BASE COUNT         1055 a          556 c          576 g         1084 t
ORIGIN      
        1 atcggcacct aaggtaagtg ctgcaccggc acctgcaccg gtaaatatgt tgcggctgcc
       61 ccagttttgt aatacggtaa ctccgttaag gcggtatgcg ttatttatgg cgtttacttg
      121 tatgtttata tcgttgagta cgtctaatct tgaagtgggg ttagcaacac caatacctac
      181 attgcctccg ttaagtatgg tcattcggtt aacacttggc gtgcctgtta aaaaatttat
      241 attttgtgtg ccgcggtgtt ctatgtttaa attatacaag tttgactgag cagaattatc
      301 ccaaccaaca taccatgttg ctgaattatt cgtatgattc aacacatcaa cctgactata
      361 aacattaatg tttataagtg tcagcagaaa aatgtaagtg atttttttca ttctatataa
      421 tttttaattg cgattatctt ttttgattcc attttacttt tttcataaac acataaaaaa
      481 tatatagtgc tcgatccagg taacatgata tcaatttgtt gatccattgt cttagtagta
      541 tttaacaatt gaccggatac cgaataaatt aatatttctt tttcaatata attatcattg
      601 aatataacct ttaatttatc atccttaaga taactatttt taatgatggt ttgaggctga
      661 tttgaagtac tatgatttaa accagtatag ttgtcacaat aaactgaatc agtggacatg
      721 catatatcat caaaataata gtatgcgaat gttctattac taccaacagt atctgtcaag
      781 gagtaatcga aaaaatttcc aagcattaaa tattgatagt tactgtcagc aacaaaagaa
      841 agttgaattt tactccaccc agtagtatct ataatgatcg agtctgagta gattaaggat
      901 gaattattta ccgggtaagg gttattaaat gagtacggtg tcatcgttaa cattattcca
      961 aatttattta ttgcgaaaag cgtattatca gcaagactta tataaaaggt aacaaaatat
     1021 ttttgtccaa ttatggtagg agttagtagc gttgttccaa tgtactccca tcccaaaagt
     1081 ccattaatgt cataaggtat taaccccgca taagcattac cggtgcgagg aaactgatag
     1141 cctaaaacgt tgtttggaac accaacaatt cctgaatata aattgcaaca aatattaaaa
     1201 taatcgggtg ttgaccggca agaataccag ttatcagcct tgctgatttc aaacgcagat
     1261 gttgggcaac tcaccgtatc ctcaaaactt gggttgggta caaggtttag ttgtgcacta
     1321 caaatacgtg caagcagcat taaaaatatt atgagtaatt ttttcatgat gtgctgtttt
     1381 aaacttgtta agtaaaattc tgtaactgca aaacaaaatg gcagtgcgaa aagtgttcag
     1441 taaaagtagt atgggaagtg atacaggcaa ctaaattgcc atttaattac ataaaggtaa
     1501 cttgggcgat gtgcctgcaa tgattcaaat tgtggggctt attaaattat actcgcttta
     1561 ttttgtacat cgtttgtgac ctcgcttacc aatcaaaaat ttaatgttag ctgtgaaaca
     1621 acatttggct tatttttttt ccatcaggct tcaaaattat ttaagtaatt tcaaacttca
     1681 attgagtgtt cgcggggacg caaaccgtgc acaaaaatca aagtttaggt ttttgttttt
     1741 taaagctcaa attacagcgg gtaaaaaaat cccaaacagt tagcaaatat ctatttggga
     1801 ttttttcctt aggtgttaat atctattgca ttctttaaaa gctttaccct actccacctt
     1861 caccatcttt ttggtaacgg aagcttttcc atcggctaat aaggtataag tatatacacc
     1921 agcacttagg ttactgatat actttataat tagaaaaaat ttattttact gattatttaa
     1981 aacctttaat gaaaaagata atcctgcttt tctttctaat taattcggta gctttaaaag
     2041 cgcagcatga cagcacctat tttaaaaaat atagccacaa acttgcattg ggtttctttt
     2101 ctacgttgcg caattacaat ttacgcatta actcaaacat cagtaaggat gcaggtttgc
     2161 ctgatgttga ttttgcaacc aatggccgtt tgcaatgggg tttggctttt ggttatgaca
     2221 aaatcagatt aggtattgca atcggtaaaa gtgccaacaa tgatatttat gggcaatcgc
     2281 gtacacagaa tatagcggct gatattggtg gcaatgccat ttttattgct gttggctacc
     2341 ggagatatac aggtatgtat gataaaaatt ctaccggcta tatgcctgca aacgggccaa
     2401 ctttttatat tgatccttca tgtatttttt caatcataaa aaatatgctt ttggcgctgc
     2461 atacatggca aatcagcgac aaataaaatc agcagcttcc tggttggtaa cttccaatat
     2521 tcatcatgaa aaaattaaaa cagcagcaac aattatcccg cctccattgg ctagttatta
     2581 tggaggtgat gcgcaattgc aaacactgca atggtggggc ataaatatgg gtgccggtgc
     2641 aaccggtaat ttggtaattt taaggaagtt gtttatacat ggcactattt cctttggtgc
     2701 cgaaacacaa ttcagagaat acgtatataa cacaggttat aataaaaaag ttactgcaat
     2761 gggatttgcc ggtgatttta aagccggtat cggatttaac agcgaatcgt ttttcatttc
     2821 gataaataac aatattgatt accactcggt aagcttgtca aaccttaaat tagtcgaatc
     2881 gctggtaacg ggttatttaa taattggcta ccgctttaag gtaaaagatc cgcctttata
     2941 tcaaaagttg agagaaacca gattatataa atctctttag atttaatttt tataaaaacg
     3001 ttttcctgca aagccgttaa ccgcatttac ctgtagataa tacacacctt gcggccataa
     3061 gcttatgtca attaagttgt gttgcagttt gacataataa acttacctaa ctcaatttat
     3121 gccaaattac aaatgggggg ttatgcatat ttgttttaaa aatgccatcc tgattgatgc
     3181 cgcaaaagta tggcttttgc ctgcatcggc acattaaact aaagtaatta aattgatact
     3241 taaatagcct taaaacaaac ttactaaccc c
//
LOCUS       DAOMAA010000094         3270 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_22297_length_3270_cov_3.104821, whole genome shotgun sequence.
ACCESSION   DAOMAA010000094 DAOMAA010000000
VERSION     DAOMAA010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22297_length_3270_cov_3.104821"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1460
                     /locus_tag="PLO59_01990"
     CDS             <1..1460
                     /locus_tag="PLO59_01990"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HQU99895.1"
                     /translation="VTFSDDQGAYQDDFRITNGGSYAEFSNATSGQTLMTFGLGQFTG
                     LVGVGTNAPTEKLTVVTTGPASGLGIYSNNASRTGLEMGRNNASDLSIGIADAPGSYV
                     TGSVAGDAIFRNNSNTNKLLFAAGGGGAPVTMAVTSKYVGIGTTTPASKLHIVDTTSN
                     VVGALVGLHSPNMPTGGLAGFYFGKNTTFTGNQAEIRYEYYGDLDNRNGYAFGLNNIQ
                     PFVRFTRNQRVSIGGNSTPGGQFELSLDQGRKPSTSTWTITSDERLKNIDGSYTKGLD
                     AIMQLNPIKYHYKNVGERKFDAQVLNTQAIGFSAQEVKKIFPESVGTDEDGYLNLNMH
                     AILVAQVNAIKEQQSEIESQKKLIDSKDSKINDMQNQINAMQECLESLCKAAQQNTEA
                     KVATPVMHNVLFQNQPNPFNQVTTIRYQLVKAENGSQIIIRDLNGNLVKTLDLGNLAK
                     GEVTVNANELAAGTYTYTLVANGQSLDTKLMVVTK"
     gene            complement(1607..1861)
                     /locus_tag="PLO59_01995"
     CDS             complement(1607..1861)
                     /locus_tag="PLO59_01995"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQU99896.1"
                     /translation="MQKAAALIHLTDSAFCKYFKRISGKTFSDYVNDIRIAHACELLI
                     ETDKTIAQIANESGYETLTYFNRIFLKKKNIRPNAFRKLS"
     gene            complement(1905..2309)
                     /locus_tag="PLO59_02000"
     CDS             complement(1905..2309)
                     /locus_tag="PLO59_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99897.1"
                     /translation="MTSGGAAYASFARDAVYAAVMLILNGIVGLCLYIGGRKFHTQTF
                     SPHSVKIALVSLVSIVAFTMILPTFTKDISGPYYTEAQLMFEFFACLVIYIAFIAAQT
                     VRHREYFITNSNEPVNKSENVSNQTKQSVSYK"
     gene            complement(2331..2558)
                     /locus_tag="PLO59_02005"
     CDS             complement(2331..2558)
                     /locus_tag="PLO59_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99898.1"
                     /translation="MNKIRKLYPWTTSIPILVCILYASGLLDNNFILQMVAGILLLLS
                     VMSAVHHSELIAHRVGEPYGTIILAVSVTII"
     gene            complement(2754..>3270)
                     /locus_tag="PLO59_02010"
     CDS             complement(2754..>3270)
                     /locus_tag="PLO59_02010"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU99899.1"
                     /translation="SVIISVSSLPAVTINTTADTICLQSAPVSLTGNPINGIFTGNGV
                     SGNLFYPDSAGVGLHTVYYTYIDSTNQCSNTDSVAFFVDACLYIPINNNGYLNIYPNP
                     AQNKIYLEKHFVETDIRFVLRDYAGKTILYKTCSPTTSEEINIENIPSGVYSIEIRTN
                     SKTYFSKIVIL"
BASE COUNT         1086 a          563 c          613 g         1008 t
ORIGIN      
        1 aagtaacctt tagcgatgat caaggtgctt accaggatga tttcagaatt accaacggtg
       61 gcagctatgc cgaattttcg aatgctacca gcggacaaac gttaatgaca tttggcttgg
      121 gtcagtttac aggcttagta ggtgtaggca ccaatgctcc caccgaaaaa cttactgttg
      181 taactactgg tcccgcaagc ggtttgggta tatactcaaa caatgcttca cgtaccggtt
      241 tagaaatggg tcgcaacaat gcatccgatc tttcaatagg tattgctgat gctccgggta
      301 gttatgttac aggtagtgtt gcaggcgatg ctatatttcg taataacagc aacaccaata
      361 aacttttatt tgcagcaggt ggcggtggcg ctcctgtaac aatggctgtt acatcaaaat
      421 acgtaggtat tggtaccaca acaccagcat ctaaattgca catagtagat acaacttcaa
      481 atgttgtggg ggctttggtt ggtttgcatt cgcccaatat gcctacaggc ggtttagccg
      541 gattttattt tggaaagaat acaacattta ccggaaacca agccgaaata cgatacgagt
      601 attatggcga tttggataac agaaatggtt atgcatttgg gttaaataac attcaacctt
      661 ttgtaaggtt tacacgcaac cagcgcgtta gcataggtgg caattctaca cccggtggtc
      721 agttcgaatt aagtttagat caaggccgta aaccatcaac cagtacctgg actattactt
      781 cggacgagcg tcttaaaaat attgatggtt cttacacaaa aggcttagat gctattatgc
      841 aattaaatcc aattaagtat cactataaaa atgtaggcga acgtaaattt gatgcacagg
      901 tgttaaatac gcaagcaatt ggatttagtg cacaagaggt aaaaaagata tttcctgaaa
      961 gtgtaggtac tgacgaagat ggttacttaa acctaaacat gcatgccatt ttagttgccc
     1021 aggttaatgc cattaaggag cagcaatcag aaatagaatc acaaaagaaa ctgattgact
     1081 ctaaggattc gaaaattaat gacatgcaaa accaaatcaa tgccatgcag gaatgtcttg
     1141 agtcgctttg taaggcagca caacaaaaca ccgaagcaaa agtagccaca ccggtaatgc
     1201 ataatgtttt atttcaaaac caacccaatc catttaatca ggttacaact atacgttacc
     1261 aattagtaaa agctgaaaac ggaagtcaaa ttatcatacg tgatttaaat ggtaatttgg
     1321 ttaaaacgct tgatttagga aaccttgcaa aaggtgaagt aaccgtaaac gctaatgaat
     1381 tagctgccgg cacttacact tataccttgg tagctaatgg ccaaagttta gacaccaaat
     1441 taatggtagt tacaaaataa ttagttatta aacttttcga aattctagca ggctgtcaat
     1501 tgtaaaattg acagcctttt ttatgcctta aaaagcagtt gagttttaaa ttaattaatg
     1561 atactacggc atttgtatcc aatttttacg gccaacattt taaagtttac gaaagctttc
     1621 gaaaagcatt tggcctaatg tttttctttt taaggaatat acggttaaaa taggttagtg
     1681 tttcataacc gctctcattg gctatttggg caatggtttt gtcggtttca attaataact
     1741 cacatgcatg tgctatgcgt atatcgttta cataatctga aaatgttttg ccgcttatcc
     1801 gtttaaaata tttgcaaaag gcgctatcgg ttaaatgaat taatgccgct gctttttgca
     1861 atgaaacttg ttgcttgtat gtgctttgaa catattgaaa aactttattt atacgaaact
     1921 gattgctttg tttgatttga tacgttttcg cttttattta ccggctcgtt tgaattggta
     1981 ataaaatatt cgcgatgcct tacagtttgt gctgcaataa aagcaatata gattaccaaa
     2041 catgcaaaga attcaaacat caattgcgct tctgtgtaat aggggcctga gatatctttg
     2101 gtaaacgtag gtaatatcat agtaaaagcc acaatagata ctaatgatac caatgcaatt
     2161 tttaccgaat gtggtgagaa tgtttgtgta tgaaatttac gaccaccaat atataagcaa
     2221 agaccaacta taccattaag tattagcatt actgctgcat aaaccgcatc gcgtgcaaat
     2281 gaggcataag cggcaccacc acttgtcata agagaaataa taatagctac ttatattatc
     2341 gttacagaaa cagctagtat aatagtaccg tatggttcgc ctactctgtg ggcaattaat
     2401 tcagaatgat gtacagccga cattacactt agcagcagta aaatacctgc caccatttgt
     2461 aaaataaaat tgttatctaa aagacctgat gcgtataaga tacaaactaa tataggtatt
     2521 gatgtagtcc aaggataaag tttcctgatt ttattcatta aatgtgattg ctgtatttta
     2581 ttaaatcaca aagttattta taatctctgt tgcatcagat taagttaatc cgatttggta
     2641 caaatatttt tatttaaacc aagtaacata tgcaatatgt ttttggctca tatatgcctg
     2701 ctgaaaaatt aataaatgaa tgttcgacat aattgcgctt taaaaaatag caattataaa
     2761 atcactattt tagaaaaata tgttttgcta ttcgtcctga tttcaataga ataaacccca
     2821 cttggtatgt tttcaatatt tatttcttct gaggttgtag gcgaacaagt tttatacaat
     2881 atcgtttttc cggcatagtc gcgcaaaaca aatcgaatat cggtttcaac aaaatgcttt
     2941 tcaaggtata ttttattttg tgccggatta ggatagatat ttaaatagcc attgttattt
     3001 ataggaatat agagacaagc atctacaaaa aatgcaaccg aatcagtgtt cgaacactga
     3061 tttgttgaat cgatataggt gtaatataca gtatgtaatc caacacctgc agagtcggga
     3121 taaaataaat tgccactaac gccattgcct gtaaaaattc cgttaatcgg attacccgtt
     3181 agtgaaaccg gtgctgactg caaacaaatc gtatcggcag tggtatttat agttactgca
     3241 ggcaaagagc taactgagat tataacagaa
//
LOCUS       DAOMAA010000095         3268 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_22320_length_3268_cov_3.045129, whole genome shotgun sequence.
ACCESSION   DAOMAA010000095 DAOMAA010000000
VERSION     DAOMAA010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22320_length_3268_cov_3.045129"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="PLO59_02015"
     CDS             <1..668
                     /locus_tag="PLO59_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQU99900.1"
                     /translation="EDDTNFGSVLKNYLELNDYDVTLARDGQEGLDRFYKTKHDLCVL
                     DVMMPKKDGFSLAKDIRQHNANVPIIFLTAKSMKEDMIEGFKSGADDYLTKPFDSEVL
                     LFKIKALIKRASESFVKPDQTQFEIAGYKFDYNMRILHFGNSQVNLSPKEADLLKMLC
                     HHMNDVLPREKALKAIWGDDNYFTGRSMDVFITKLRKYLKEDSRIDIMNIHGKGFKMN
                     VNV"
     gene            complement(788..>3268)
                     /locus_tag="PLO59_02020"
     CDS             complement(788..>3268)
                     /locus_tag="PLO59_02020"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HQU99901.1"
                     /translation="DTVNITTNLTPFVQINGNDSICADNAQTLLATGAISYTWQNASN
                     PGVIIGISDTLQLTPNQSGTYIVTGTTSPNCSSSDTITITINQLPTIIATAATDSICK
                     GDTITLFAQGGVSYTWVDASNPGVVISTNNALNIFLNNTATYLVTGTNALGCVNNDTI
                     TINAIDLPNTPTLTGDSIICDTNNIYLYIANSASVGLSYNWIISNGIIVNGQGTDSIM
                     VQWNTSGPYQLTCIGTNVLGCNAVPTGISAILGNHITLPIPTGNVVVCDNDTVTYSAL
                     ANPNFVYNWIVNGGTILSGNGTSSVSIIWNLQPPTTGQIWYTVSTIAPDTLCFTVSDT
                     LEVTINNAPAPPVLTVPTAMCLLDTFILNVTPVAGNTYNWAISNGSIINGNGTAQITV
                     VFQSAGMQMVTVFATSAAGCESALAIANINVNALPAANAGNDTTACIGELISLNANGG
                     SSYLWSPATGLSSNTISNPTVTITGNITYTVLVGDNNGCSALDSIQITMLPQLTATAT
                     ADQNSICQGNTAILTATGGNNFAWIPATGLSSSNNAITNANPLTTTTYSVIVNNTNFC
                     ADTATVTLTVNALPNISTNNNLNGCNGDSVLLMAQGATTYSWLPISGLSNSNVSNPMA
                     LVSGNTTYTVTGTDASGCTSTATVAIVANTQPDAAFDTSYLAVNCNGLLVSFTNQSTN
                     ASTYLWDFGNGNTSNDVNPQHSFGFGNTYSVTLFAYNGNCVDSTVQIINQPTLNTYFD
                     SIPNVFTPNGDGKNDCFNVTAHANLAACTSVEIYNRWGNLLYKSNDTNACWDGKNENT
                     GEAVPKGTYIYLIKVNDQKFNGTVNLLK"
BASE COUNT          983 a          598 c          675 g         1012 t
ORIGIN      
        1 tagaagacga taccaacttt ggcagtgtat taaaaaacta tttagagctt aacgattatg
       61 acgttacgct tgcgcgtgac gggcaagagg gtttagatag attttataaa accaaacatg
      121 acttatgtgt tttagatgtt atgatgccaa aaaaggatgg ctttagtttg gccaaggaca
      181 tcagacagca taatgccaac gtacccatta ttttccttac agctaaaagt atgaaggaag
      241 atatgattga aggttttaaa tcgggtgccg atgattacct aaccaaacca tttgatagcg
      301 aagtgctttt gtttaaaatt aaagcattaa taaaacgtgc cagcgagagt tttgtaaaac
      361 ccgatcaaac gcaatttgaa attgcagggt ataaatttga ttacaacatg cgcatacttc
      421 atttcggtaa ctcgcaagta aatctttcgc ccaaagaagc cgatttgctt aaaatgcttt
      481 gtcatcacat gaatgatgtg ttgccacgcg aaaaagcatt aaaagctatt tggggggatg
      541 acaactactt tacaggacgc agcatggacg tgtttataac caagttgcgc aagtatctga
      601 aagaagatag ccgcatagat attatgaata tacatggcaa aggatttaaa atgaatgtga
      661 atgtgtaagc acatcagcaa tgtataacaa cagccaataa aaggtttagt tttaatccaa
      721 taaataaatg caaaacggct aacttcagat tcgagttagc cgttttgcat ttatggagcc
      781 tcaagcgtta ttttaataag ttaaccgtac cattaaattt ctggtcgttt acttttataa
      841 gataaatata ggtgccctta ggaaccgctt cgcctgtatt ttcgttttta ccatcccaac
      901 atgcattcgt atcgtttgat ttatataata agttacccca gcgattataa atttcaaccg
      961 aagtacaggc cgctaaattg gcatgagctg ttacattaaa acaatcattc tttccatcac
     1021 cgtttggtgt aaacacattg ggtatgctgt caaaataagt attaagtgtt ggttgattaa
     1081 taatttgtac ggtactatca acacaattac cattataggc aaacaaggtt acactgtaag
     1141 tattgccaaa gccaaaacta tgttgagggt ttacatcgtt tgatgtattg ccattgccaa
     1201 agtcccataa ataagttgat gcattggtgc tttgatttgt aaatgatacc aacaatccat
     1261 tacaatttac agccagataa gaagtatcaa atgctgcatc aggttgcgtg tttgcaacaa
     1321 tggcaactgt tgctgtactt gtgcagcctg atgcatcggt accggttacg gtataggttg
     1381 tattaccact aaccaatgcc attgggttag atacattact attgcttaag cccgaaatgg
     1441 gtagccagct ataggttgtt gcaccttgtg ccataagtaa tacgctatcg ccattacaac
     1501 cgtttaaatt attattggta cttatgttag gcagtgcatt tacggtaagt gttacagttg
     1561 cagtgtcggc acaaaaattg gtgttgttta caattacact ataggtggtt gtagttaatg
     1621 gatttgcatt ggttattgca ttgttcgaac tgcttaaacc tgtagcaggt atccaggcaa
     1681 aattattgcc gcctgtggca gtcagtatag cagtattacc ttggcaaata ctgttttgat
     1741 cggccgttgc tgttgcagtc agttgcggta acatggtaat ttgtatgcta tctaaagccg
     1801 agcagccatt attatcgcca actaaaaccg tataagttat gttacctgta atagtaacag
     1861 tagggtttga aatggtattg gatgaaagac ctgttgcagg agaccacaaa tagctactac
     1921 ctccattggc gttgagtgaa attaattcgc ctatgcatgc cgtagtgtcg ttgccggcat
     1981 tagctgcagg caaggcatta acattgatgt tggctatagc caatgcactt tcgcagcctg
     2041 cagccgatgt tgcaaaaaca gtaaccattt gcatgcctgc actctgaaaa acaacagtta
     2101 tttgtgcagt accattgccg ttaataatac tgccgttact aatggcccaa ttgtaagtgt
     2161 tgccggctac aggtgttaca tttagtataa atgtatctaa taaacacatg gccgttggaa
     2221 ccgttagtac cggtggtgct ggtgcgttgt ttatagtaac ttccaaagta tcgcttactg
     2281 taaaacacaa ggtgtcaggc gcaatggtgc ttaccgtgta ccaaatttgt cctgtagtgg
     2341 gtggctgtaa attccagatg atacttacgc tcgaagttcc gttgccacta agtatagtac
     2401 ctccgttaac tatccagttg taaacaaaat taggattggc taatgcactg taagttaccg
     2461 tatcgttatc acaaactact acatttccgg ttggtatagg caacgttata tgattaccca
     2521 atatggccga gatgccggtt ggtactgcat tacatcccaa tacattggtg ccgatacaag
     2581 taagttgata tggtccggaa gtgttccatt gcaccatgat actatcagta ccctgtccat
     2641 taactatgat accattacta ataatccaat tgtaactcag gccgacactt gcactattgg
     2701 caatatataa gtatatgtta ttggtatcgc aaattatact atcgcctgtt aatgtaggtg
     2761 tgttaggtaa atcaattgca ttaatggtta tcgtatcatt atttacacag cctaaagcat
     2821 tggtgcctgt taccaaatag gttgcagtgt tgtttaagaa aatgtttaat gcattattag
     2881 tacttataac aactcccgga ttactggcat caacccaagt gtaactaaca ccgccctgcg
     2941 caaaaagggt aatcgtatcg cctttacaaa tactatcagt ggcagcagta gcaataatgg
     3001 taggcagttg attaattgta atggttattg tatcgctgct gctacaattt ggggatgttg
     3061 tacctgttac tatataggta ccgctttgat taggtgttaa ctgtaaggta tcggatattc
     3121 caatgattac accggggttg cttgcgtttt gccaggtata actaatagca cccgtagcca
     3181 ataatgtttg cgcgttatcg gcacaaatag aatcgttgcc attaatttga acaaaaggtg
     3241 ttaggttagt tgtaatatta accgtatc
//
LOCUS       DAOMAA010000096         3258 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_22446_length_3258_cov_2.092413, whole genome shotgun sequence.
ACCESSION   DAOMAA010000096 DAOMAA010000000
VERSION     DAOMAA010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22446_length_3258_cov_2.092413"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..3225)
                     /locus_tag="PLO59_02025"
     CDS             complement(142..3225)
                     /locus_tag="PLO59_02025"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HQU99902.1"
                     /translation="MYPLTKVLIRTIFILLLTCSYFASIAQTKQVLQFDAITIDDGLS
                     QGMINCIMRDSYGFMWFATKDGLNRYDGYEFVVYKHDISDSNSICDNMVTYIFEDRQR
                     RLWVGTGSGLSIFSRNTERFTNLTPKINSQLTCANGLVTSIGQDIYGDIWVATYNGLT
                     QWHETITNGQSNFLSTCYFKNEVNLHIDSKSQIWVSVLSDALYQIVPRKNNKPVINKL
                     PRSFFGDSLKVSNKRPVNIFVEDTLNQKLYFLYRQGITQLDLNSGKRNYLKSPMVTYG
                     NFERQAYIDKTEQIWYVESNVLLKYNVPQQIITNITASDSHKQQSLQNTSNVYCDNSG
                     LIWIGTKGYGILKYHPRIEQFNFIAAGSMGFMSAAPGNKVVITKAFSFLSTFNIAGKK
                     IETIPDSTFINEVDFQNFGISTSAVFEDTNIVWLSKGKLTKIDLRTKKVKQYNNGSVE
                     NFPVFLSDSMVYIGTPHSFSVFNPRTETFTNYPYPVKISQMPYNFLQHIYRAPNGIFW
                     LATAQGLFALDIVKNKWQHYHNQKGNLQSLSVDMVYSICADAVHPEKYLWLGTNGGGL
                     NKFDIETGQCKIFNTKNGLPNDVVYGILSDENNNLWLSTNLGLACFNPNSGLFNNYQA
                     KDGLQSNEFNRFAYCKTADGCLFFGGINGLNYFYPAQIKPLKTQPQLLITAIYINNKK
                     PLSFHKSLILSSPAYLTNEVKLTPDNNIITFEFATTDYIASTKNKYQYKLDGFDKDWV
                     QSGSVRLATYTNLDPGTYTFTVKGTNIDGIWSATSQTVTIKILAPWYSTWWFAAIVVS
                     IVAALGFFLYKFRFYRALEIANVRNRIARDLHDEVGSNLSSITIYNDVAMQKNKQTEV
                     EPLLQKMGFYAQSSLEAMNDIVWMITTNNDKFENIFARMREHAVNLLDEKCILHINFD
                     EKLYAEKLQMDERRNFYLIYKEALNNILKYAKCQNVWIELKLLNTLVLLRIKDDGIGF
                     EMTDKELSKVLSSNGGNGLINMQNRAALLQGELTLKSAPGQGTVMVLMFQLKHSW"
BASE COUNT          999 a          623 c          531 g         1105 t
ORIGIN      
        1 gcagttatca aatgtaccca cacattccat atcggaactt gaatcaatca gcagtcgtaa
       61 agcttcttgc cgtgctttgt tatcatcaaa tacaataact ctaattttca taattcaacc
      121 gttttaagtt tgtataaatt actaccagct atgtttcaat tgaaacatca gaaccataac
      181 ggtgccttgt cccggtgccg actttaaagt gagttcgcct tgcagtaaag ctgctctgtt
      241 ttgcatatta attaatccat taccaccatt actcgacagt actttcgata attccttgtc
      301 cgtcatttca aaaccaatac catcatcttt aattcttaat aaaaccaaag tgtttagcag
      361 ctttaattca atccatacat tttggcattt ggcatacttt aaaatattat ttaaagcttc
      421 cttataaatc agataaaaat ttctgcgctc gtccatttgc agcttttctg cataaagctt
      481 ctcgtcaaaa ttaatatgta agatgcactt ttcgtctaac aggtttactg catgttcgcg
      541 catacgtgca aaaatgtttt caaacttatc gttattggtg gtaatcatcc acacaatatc
      601 attcatagct tctaacgatg actgtgcata gaagcccatt ttttgcagca aaggttccac
      661 ctctgtttgc ttgttctttt gcattgccac atcgttataa atggtaatgc tgcttaagtt
      721 acttcctact tcgtcatgta aatcgcgggc aattctatta cgcacattgg caatttccaa
      781 tgcacggtaa aatctgaatt tataaaggaa aaagccgagt gcggctacta ttgaaactac
      841 tatggcggca aaccaccacg tactgtacca tggtgctaaa attttaattg ttacggtttg
      901 gcttgttgca ctccaaatgc catcaatatt ggttccttta actgtaaagg tataagtgcc
      961 ggggtctaaa tttgtgtaag ttgctaaacg cacactgccc gattgaaccc aatctttatc
     1021 aaaaccatct aatttatatt ggtatttgtt tttggtggat gctatataat ccgttgtagc
     1081 aaattcgaaa gtaattatgt tgttatcggg cgttaattta acttcgtttg taagatatgc
     1141 cgggcttgat aaaattaaac ttttatggaa cgacagaggt tttttattgt tgatataaat
     1201 ggccgttatt aataactgtg gttgggtttt tagtggttta atttgagccg gatagaaata
     1261 atttaatcca tttataccac caaaaaacaa acaaccatca gccgttttgc aatatgcaaa
     1321 acgattaaac tcattgcttt gtaatccatc ttttgcctgg tagttattaa acaaaccgga
     1381 gttcggatta aaacatgcca aacctaaatt ggtgcttagc cacaggttat tattttcatc
     1441 gcttagtatg ccataaacaa cgtcattagg caaaccattt ttagtattaa aaattttgca
     1501 ttgtccggtt tcgatatcaa atttatttaa gccaccccca ttggtaccta accacaaata
     1561 tttttcaggg tgtaccgcat cagcacaaat actataaacc atatctacgc ttaacgattg
     1621 caagtttcct ttttgattgt gatagtgttg ccatttattt tttacaatat caagtgcaaa
     1681 taacccttga gctgtagcca gccaaaaaat accattaggt gcacggtata tatgctgcaa
     1741 aaaattatat ggcatttgac taatttttac aggatatgga tagttggtaa aagtttcggt
     1801 tcgtggatta aatacactaa aactgtgggg tgtgcctatg tacaccatac tatctgataa
     1861 aaacaccgga aaattttcaa cgctgccgtt attatattgt tttacttttt ttgttcggag
     1921 atcaattttg gtaagctttc cttttgatag ccaaacaatg ttggtatctt caaaaaccgc
     1981 tgaggtagaa attccaaaat tttgaaaatc aacctcatta ataaaagtac tgtcaggtat
     2041 ggtttctatt tttttgccgg caatattaaa agtacttaaa aatgaaaatg cctttgttat
     2101 tacaacctta ttaccgggtg ctgccgacat aaatcccatg ctacctgcgg caataaaatt
     2161 aaattgctcg attcgcggat gatattttaa aataccgtag cccttggtgc ctatccaaat
     2221 taaaccactg ttatcgcaat aaacattgct tgtattttga agcgattgct gcttatgact
     2281 atcagaagct gtaatgttgg ttatgatttg ctgtggcaca ttgtacttta gtaatacatt
     2341 actttccaca taccatattt gttctgtttt atcaatatat gcctgtcttt caaaattgcc
     2401 ataagtaacc atgggcgact ttaaatagtt tctttttcca gaatttaaat ctaattgtgt
     2461 gatgccttgc cgatacaaga aataaagttt ttgattaagg gtatcctcaa caaaaatatt
     2521 taccggcctt ttattgctaa cctttaatga atctccaaaa aatgagcgtg gtaatttatt
     2581 aataaccggt ttgttgtttt tacggggcac tatctgataa agcgcatcgc ttaataccga
     2641 tacccaaatt tggctcttac tatctatatg cagatttacc tcgtttttaa aataacaagt
     2701 tgataaaaag tttgattggc cattggttat cgtttcatgc cactgcgtta atccattata
     2761 cgttgccacc catatatcac cataaatatc ctgcccgatt gaagttacca ggccgtttgc
     2821 acatgtaagc tgactattga ttttaggagt taaattggta aagcgttctg tattgcggct
     2881 aaatattgaa aggccgctgc ctgtaccaac ccacaatctg cgttgcctgt cttcaaaaat
     2941 ataggtaacc atattgtcac atatactgtt gctatcggat atatcatgct tgtaaaccac
     3001 aaattcataa ccatcgtatc tgtttaagcc atctttggtg gcaaaccaca taaagccata
     3061 actatcgcgc ataatgcagt taatcattcc ctgcgataaa ccatcatcaa tagttattgc
     3121 atcaaattgc aatacctgct tggtttgtgc aatacttgca aaataactac aagttaaaag
     3181 cagaataaat attgtacgta taagtacttt agtgagcgga tacatacgca aacaatgtat
     3241 gcatgcaaaa aaaataat
//
LOCUS       DAOMAA010000097         3255 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_22481_length_3255_cov_3.130937, whole genome shotgun sequence.
ACCESSION   DAOMAA010000097 DAOMAA010000000
VERSION     DAOMAA010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3255
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22481_length_3255_cov_3.130937"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1184)
                     /locus_tag="PLO59_02030"
     CDS             complement(<1..1184)
                     /locus_tag="PLO59_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99903.1"
                     /translation="MKKTITHLAVGALLCTAGTAQAQWLVGGNALGANGNFGTTTNVG
                     VNFITNNLSRMTLTNGGLLGIGTTAPTFLLDVQSAGNASASFKSTGGTANLIIDRGNA
                     AATSSVSYRTGGSPTWQTGTLGTNNFAIRNIALGAASISINAADNFVGIGTNAPTARL
                     HVLGNVNASGGVTTVDTVLNVNGNLNVSGAIRIGTIETLTDEGGFTMGCNSDFVSVTD
                     GLNNLGNSGARWRNLMLRDQATIGTASSRNIATDSVSIRALNNGVASNLAVTASNFTL
                     DNAGGTQFINSGSGNNMTISGVGGGSVGLKLNRVGTGSFDFNLVNNFGILEYRGSTDE
                     FATVNNLFMSMSLSSGNIGMGVAASGTYKLSVCGTVRATELRLETGWCDYVFADDYRL
                     KS"
     gene            1500..2495
                     /gene="pdhA"
                     /locus_tag="PLO59_02035"
     CDS             1500..2495
                     /gene="pdhA"
                     /locus_tag="PLO59_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HQU99904.1"
                     /translation="MSKTKFTKETYLMWYEQMLLMRRFEERAGQLYGMQKIKGFCHLY
                     IGQEALMAGAMSVLRPEDPMITAYRDHAHALAKGVSARAVMAELFAKATGCSKGKGGS
                     MHLFSKEHHFYGGHGIVGGQIPLGAGIAFAEKYKGTDNVCVCYMGDGATRQGALHEAF
                     NMAMTWKLPVVFIIENNNYAMGTSVERTSNVTELWKLGLAYDMPSQPVDGMTCEDVHE
                     AMDKAVKHARAGNGPSLLEINTYRYKGHSMSDPAKYRTKEEVERYKAEDPIEKVLKTL
                     LDKKYATQADIDVIIKRVNDVVEDSVTFAEESPYPDPSELYKDVYVQDDYPYIMD"
     gene            2530..>3255
                     /locus_tag="PLO59_02040"
     CDS             2530..>3255
                     /locus_tag="PLO59_02040"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HQU99905.1"
                     /translation="MAEIVRMPKMSDTMTEGVVAKWHKKVGDVIKSGDLVAEIETDKA
                     TMEFESYQTGVLLYRGVEEGKAASVNGILAILGNAGESFDELLAAEKTNAISETQIIT
                     KEPEPAKAPPTSEAAPIAPKPIATPAIKTTINLTGSSDDGRIKASPLAKKLAADKGLD
                     VRLLKGSGDNGRVIKRDIEWYKPGNMVETNYYTQAVTSESAEDVPVSQMRKTIAKRLS
                     ESKFTAPHFYLTMEINMDNAMAAR"
BASE COUNT         1058 a          669 c          631 g          897 t
ORIGIN      
        1 aatgatttta aacgataatc atcagcaaaa acataatcgc accagcctgt ttctaagcgc
       61 aactcagtgg ctctaacggt accgcatacc gatagcttat aagtgccact tgcggcaact
      121 cccataccta tgttaccact gcttaaactc atactcataa aaaggttatt aactgtggca
      181 aattcatcgg tactgcccct atactcaagt atgccaaaat tatttaccag attaaagtca
      241 aacgatccgg ttcctactcg gtttaacttt aacccaacac tgccgccacc aacaccagat
      301 atagtcatgt tattaccgct accgctattt ataaactggg ttcctcctgc attatccaat
      361 gtaaagttag atgctgtaac tgctaaattg ctggctacgc cattatttaa tgcacgtatc
      421 gaaacactat cagttgcaat attacggctg cttgcagtac caatagtagc ttggtcgcgc
      481 aacattaaat tacgccatct tgccccacta tttcctagat tatttaatcc atcagttacc
      541 gacacaaaat ccgaattaca acccatagta aatccacctt catccgttaa agtttcaatt
      601 gtgcctatcc ttattgcacc tgatacattt aaattaccat ttacatttaa cacagtatca
      661 actgtggtta caccaccact tgcattaacg ttgcccaaaa catgtaagcg cgctgtgggt
      721 gcattggtac caatacccac aaagttatca gcagcattta tcgaaatact tgcagctcct
      781 aatgcaatgt ttctgattgc aaaattattg gtacctaagg tacccgtttg ccatgtgggg
      841 ctaccaccgg tacggtaact aaccgaagat gtagctgctg cattaccacg gtcaatgatt
      901 aaattggccg taccgcctgt tgacttaaat gaagccgagg cattgcctgc cgattgcaca
      961 tctaataaaa aggtgggcgc tgttgttcca ataccaagta aaccgccatt tgtaagtgtc
     1021 atgcggctta agttgtttgt aataaagtta acaccaacgt tggtggtagt tccaaagtta
     1081 ccgttggctc ctaaggcatt accgcctacc aaccattgtg cttgtgctgt accggcagta
     1141 caaagcaatg cacctactgc taaatgagtg attgtttttt tcattgtttt ttggttttgt
     1201 tgtttatgat aaatttcaat ttgcacaaaa ctaaaagatg gatttaaatt gttcttacta
     1261 agccgttata acctttgtta catttaaaca agtaaccatt gggttaataa aattacctga
     1321 cgtatattaa aacatacttt tacattatgc taaatcactt agtcaactac aatactttca
     1381 atattaagta ttgaccagct tattgtaaag attacatttg ttgccaccgt aataagtgta
     1441 caaactacac ttacaccatg cgcattgaat aacccatttt gattgaacct aattcttata
     1501 tgtcgaaaac aaaatttaca aaagaaacct acctgatgtg gtatgaacaa atgctgctta
     1561 tgcgcagatt tgaagagcgt gccgggcaac tttatggtat gcaaaaaata aaaggttttt
     1621 gccatttgta tatcggccag gaagcattaa tggctggcgc aatgagcgta ttaaggcccg
     1681 aagacccaat gattactgcc tatcgcgatc atgcacatgc attggcaaaa ggtgttagcg
     1741 cccgtgccgt tatggccgaa ctttttgcta aagcaaccgg ttgcagtaaa ggcaaaggtg
     1801 gtagcatgca tttatttagt aaggaacatc atttttatgg tggccatggt atagtgggcg
     1861 gtcaaattcc tttaggtgca ggtattgctt ttgccgaaaa atataagggt actgataatg
     1921 tttgtgtttg ttatatgggc gatggtgcca cacgacaagg cgctttacac gaagcattta
     1981 atatggccat gacttggaaa ctacccgttg tttttataat tgaaaacaac aattacgcaa
     2041 tgggtacatc ggttgaacgt acaagtaatg ttacagaact ttggaaattg ggtttggctt
     2101 acgatatgcc cagccaacca gttgatggca tgacttgcga agatgtacac gaagccatgg
     2161 ataaagctgt aaaacatgca cgtgcaggaa atggcccatc tttgttagaa ataaatacct
     2221 atcgctataa aggacatagc atgagcgacc cggcaaagta ccgcaccaaa gaagaagttg
     2281 aacgttataa agccgaagac ccaattgaaa aagttttgaa aacactattg gataaaaaat
     2341 atgctaccca agccgacatt gatgtaatta ttaaacgcgt aaacgatgtg gttgaggata
     2401 gtgtaacttt tgccgaagag tcgccatacc ccgatccttc agaactttac aaagatgttt
     2461 atgtacagga tgactatcct tacattatgg actaattatt aaataaaaat ttttatcaac
     2521 ttttaaaata tggccgaaat tgtacgaatg cctaaaatga gcgataccat gacagaaggt
     2581 gtggtagcta aatggcataa aaaagtggga gatgttataa agtcgggcga cctggttgcc
     2641 gaaatagaaa ccgataaagc aaccatggag tttgaatcat atcaaacagg tgtgctttta
     2701 tatagaggtg ttgaagaagg taaagccgca tccgtaaatg gcatattagc aatattaggt
     2761 aatgcaggcg aatcgtttga cgaattgtta gctgcagaaa aaaccaatgc aatatctgaa
     2821 acacaaatta taacaaaaga acccgaacct gctaaagcac caccaaccag cgaagctgca
     2881 cctattgcac ctaagcctat tgctacacca gcaattaaga ctacaataaa tcttacaggc
     2941 agttcggatg acggaagaat taaggcatca cctttggcaa aaaaattagc agccgataaa
     3001 ggtttagacg tacgcctact aaaaggtagt ggcgacaatg gccgcgtaat caaacgggat
     3061 attgaatggt acaaaccggg caatatggtt gaaaccaatt attacacaca agctgttaca
     3121 agcgaaagtg ccgaagatgt gccggtatca caaatgcgca aaacaatagc caaacgttta
     3181 agcgaaagca aatttactgc cccacacttt tacctaacca tggaaataaa catggataac
     3241 gccatggctg caaga
//
LOCUS       DAOMAA010000098         3231 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_22772_length_3231_cov_3.055101, whole genome shotgun sequence.
ACCESSION   DAOMAA010000098 DAOMAA010000000
VERSION     DAOMAA010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22772_length_3231_cov_3.055101"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..692)
                     /gene="trmB"
                     /locus_tag="PLO59_02045"
     CDS             complement(24..692)
                     /gene="trmB"
                     /locus_tag="PLO59_02045"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013306220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HQU99906.1"
                     /translation="MGKDKLRRWAELLTFKNVFQPELSYPMQSHALQGHWHEVFGNKN
                     PIVIELGCGRGEYTIGLAKANPDKNFIGIDIKGARLWRGAKTANDAGMANVAFLRIRI
                     EFIHAFFAASEIAEIWITFPDPQLKDNREHRRLTHQRFLEVYKQMLMPKGIIHLKSDS
                     AELYDFTIQNLQTQAGKLLVCTPDLYNEKKLNELQQIRTTYENIFLKAGKKITYIQFQ
                     FDNL"
     gene            899..1729
                     /gene="ygiD"
                     /locus_tag="PLO59_02050"
     CDS             899..1729
                     /gene="ygiD"
                     /locus_tag="PLO59_02050"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HQU99907.1"
                     /translation="MSNLQTLYAQQQQWPLTPLMPVLFLGHGSPMNAIEENEFVTGFR
                     QQAMLLPTPVAILCISAHWYTDGTFVTAMEQPRTIHDFGGFPPQLYQVQYPAKGSVTL
                     AIETKKLAEPIKVGLDESWGLDHGAWSVIKHLYPKANIPVVQLSIDYTKPASYHYDLA
                     TQLKQLRQHGVLIISSGNIIHNLAMADFANLNKKYGYDWAIEAHQALNKHLQNGNYDV
                     LIDYKKQNKAWQLAIPTPDHYLPLIYALALQDKKDQMLLFNDTLLAGSLSMTSVKLFQ
                     "
     gene            complement(1978..>3231)
                     /locus_tag="PLO59_02055"
     CDS             complement(1978..>3231)
                     /locus_tag="PLO59_02055"
                     /inference="COORDINATES: protein motif:HMM:NF025930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HQU99908.1"
                     /translation="MMREYILRFHDASRRTYAHPLLKNLMAETFGVMVYQEDVIKVAH
                     HFAGLTLTEADMLRRGMSGKYRGRDEFKKVEKRFFENCNAKGYPPHITAEVWRQIESF
                     AGYAFSKGHSASYAVESYQCMYLKTYYPLEFMVAVINNGGGFFSTQFYVHEARQCGAT
                     IEAPHINISYIETSISGNTITLGFNMIAELTQQVMFDLLVQRKQGHFKSLADFVNRVS
                     ISIDQLRLLIRSGAFRFTGRTKKQLLWDVHTLIGHTHKNHVQPQLFNTGLKNYILPAL
                     HHHRLDDALDEIELLGFPLCNPFLLLKHKTNLSFTAAHFAAHYGQVITIVGYLVTTKP
                     SRTKQGQAMFFATWLDSAGRFFDTTHFPLVAQQYPARNRGCYTITGRVAHEFGFYSID
                     AISMQRLDFITYNDLASDETTAHTT"
BASE COUNT          948 a          631 c          648 g         1004 t
ORIGIN      
        1 ttatacctta aagtattgtg gtgttataaa ttatcaaact gaaactgaat gtacgtaatt
       61 tttttaccgg cttttaaaaa tatgttttca taagtggttc gtatttgttg cagttcattc
      121 agtttttttt cgttgtataa atcgggtgtg catacaagta gtttaccggc ttgggtttgt
      181 aaattttgta tagtaaaatc atacaactcg gcactgtcgc tttttaaatg aatgatacct
      241 ttaggcatta acatttgttt atatacttct aaaaaacgct gatgtgttaa acgcctgtgt
      301 tcgcgattat ctttaagctg cgggtcggga aacgttatcc agatttcagc aatttcgctt
      361 gcagcaaaaa atgcatgaat aaactcaata cggatacgca aaaatgccac attggccatg
      421 ccagcatcat ttgctgtttt tgcaccacgc cacaaacgcg cacctttaat atcaatacca
      481 ataaaatttt tatcaggatt tgcttttgct aagccaatgg tgtactcgcc acggccgcaa
      541 cccaattcaa ttacaattgg atttttattc ccaaatactt catgccaatg cccttgcagt
      601 gcatggcttt gcatggggta actcaattca ggttgaaaaa catttttaaa ggttagcaac
      661 tcggcccaac gtcttaattt atctttgccc atgaggcaaa gaaataaaat tggtctgtta
      721 agcgtagtgt aaatgttatt ttctcatatc aaatacaata tttttaaatt gtatgctact
      781 tattattaat gccaaatacc gtaaaaaatt tcggtcgtat taattttcaa tttgttccgt
      841 tttttacaac atgcaacgca aacagtttat aaaatcaacc ctattaacct taggcgctat
      901 gagtaatttg caaaccctat atgcacaaca gcaacagtgg ccacttacgc cattaatgcc
      961 cgtattattt ttagggcacg gtagccccat gaatgctatt gaagagaatg agtttgtaac
     1021 cggtttcaga caacaagcaa tgctattgcc aactccggtt gctatacttt gtatatcggc
     1081 acattggtac acggatggta catttgttac tgctatggaa caaccacgta ccatacacga
     1141 ttttggtggg tttccgcccc aattatatca ggtgcaatat ccggctaaag gtagtgtaac
     1201 gttagcaatc gaaaccaaga aactggccga acccatcaag gttggtttag atgaatcgtg
     1261 gggcctcgac cacggtgcct ggtcggtaat taaacacttg tacccaaaag ccaatatacc
     1321 ggtagtacaa cttagtattg attatacaaa gcctgcttcg tatcactatg atttggcaac
     1381 acagttaaag caattgcgcc aacatggtgt tttaataatt agcagcggta acatcataca
     1441 taatttggcc atggccgatt ttgcaaatct taataaaaag tatggttacg attgggctat
     1501 tgaagcgcat caggcattaa acaaacattt acaaaatggt aattatgatg tgttgattga
     1561 ttataaaaaa caaaacaaag cctggcaatt agccattcca actcccgatc attacctgcc
     1621 attgatttac gctttggctt tgcaggataa aaaagatcag atgcttttgt ttaacgatac
     1681 gctactggcc ggcagtttaa gtatgacttc ggttaagctg tttcagtagc agtttgcgta
     1741 atgcatagta atttgtaacc ggctaaagat atctgataag tacattttta ttgttttgca
     1801 atgcaacaaa tgcaatgctg ttctattgca ttgaaaattt tggtagcaac gtatgcggtt
     1861 ttaatgagct gtattagcat actgtaggtt gctgtttaaa gcttatcaaa gcattcctgt
     1921 gtcagtaatt gtgcacaaca acagcaacaa aacagctgag tgttttttat tgctaaccta
     1981 tgttgtatgc gcagttgttt catcagaagc caggtcgttg taagtaataa aatctaagcg
     2041 ttgcatgctt atggcatcta tgctgtaaaa tccaaactca tgtgccacac gaccggtaat
     2101 ggtgtaacaa ccccggttgc gtgccgggta ttgctgtgct acaagcggaa aatgtgtggt
     2161 atcgaaaaag cggccggcac tatctaacca ggttgcaaaa aacatggcct gcccttgttt
     2221 ggtacgcgat ggtttagttg ttaccagata gcccacaatg gtaattacct ggccataatg
     2281 cgctgcaaaa tgtgcagccg taaatgataa gttggtttta tgtttgagta ataaaaacgg
     2341 attgcataat ggaaagccca acaattcaat ttcgtctaaa gcatcgtcta accgatgatg
     2401 gtgtaatgcc ggtagtatat aatttttaag gcctgtatta aatagttgtg gctgtacatg
     2461 gtttttatgc gtgtggccta tcagtgtatg cacatcccaa agcaattgtt tttttgtgcg
     2521 gcctgtaaac ctgaaagcgc ctgagcgtat aagtaaacgc agttgatcaa tactgatgct
     2581 tacacggttt acaaagtcgg ccaacgattt aaagtggcct tgtttgcgtt gtaccaataa
     2641 atcaaacatt acctgttgtg taagttcggc aatcatattg aaacctaagg ttatggtgtt
     2701 gccactgata gaagtttcga tatagctgat attaatatgt ggtgcttcta tagtagcacc
     2761 acattggcgc gcttcgtgta cgtaaaactg tgtgctgaaa aacccaccgc cattattgat
     2821 aacggccacc ataaactcaa gcggataata ggtttttaaa tacatgcact ggtagctttc
     2881 aactgcatac gaagcactat gtcctttgct aaaagcgtaa cctgcaaaac tttctatttg
     2941 ccgccaaact tcggcagtaa tgtgtggcgg atagcccttg gcattacagt tttcgaaaaa
     3001 ccgtttttca acttttttaa attcatcgcg gccgcggtat ttaccactca tgcctctgcg
     3061 cagcatatcg gcttcggtaa gggtaaggcc ggcaaaatga tgtgctacct ttattacatc
     3121 ctcctgatac accattacac caaaggtttc ggccatcagg tttttaagta aggggtgggc
     3181 ataggttctg cggctggcat cgtgaaaacg taaaatatac tcgcgcatca t
//
LOCUS       DAOMAA010000099         3229 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_22802_length_3229_cov_3.174543, whole genome shotgun sequence.
ACCESSION   DAOMAA010000099 DAOMAA010000000
VERSION     DAOMAA010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22802_length_3229_cov_3.174543"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            279..626
                     /locus_tag="PLO59_02060"
     CDS             279..626
                     /locus_tag="PLO59_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99909.1"
                     /translation="MTVKAQQRIKGSFNTVTMLVIPINPSDSVFLRTLKNTGYMAGFP
                     SFVADPGVANYTKAETDTIIQNFEKVNHPDKHFQTQGEFDLFLNKWMIELNVKRNKTN
                     RETNISDTLIIKK"
     gene            675..1022
                     /locus_tag="PLO59_02065"
     CDS             675..1022
                     /locus_tag="PLO59_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014033238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HQU99910.1"
                     /translation="MKLLTQILISTLAIMFTTYLLPHVHVDAPWHAFLVAILLAGLNM
                     LVKPILVVFTLPITIFTLGLFLIIINGIMVMMVDYLMDGFKVDNLLWAIIFSVILTGI
                     NTILQSVLGTNNR"
     gene            1195..3051
                     /gene="yidC"
                     /locus_tag="PLO59_02070"
     CDS             1195..3051
                     /gene="yidC"
                     /locus_tag="PLO59_02070"
                     /inference="COORDINATES: protein motif:HMM:NF002356.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HQU99911.1"
                     /translation="MDRNSIIGFVLIALVLVGYSYYTAPTKEELARKQMERDSLAMVA
                     QKQALDSIKLTQVITVTDTVQLSDSAKQQHLVNQLGLFATAAQGTTQRFTLENEKLII
                     TLNSQGGKVEQVELKNYTTHDKKPLLLYQADSSAMGLNFFAQNKQISTNALYFKPLGK
                     SFKVSGNGSQSFIMRLEATPEKYIDYIYTLQGNSFDVKMDITLHGVQDVIAANSNYLE
                     LDWRAALLQQELTMNAERSVSTVYYKYTDDKVDYLSESESEQKSLNEKIKWVAFKQQY
                     FSSILIAKNLFEPAIVAVQVNKNDTDIVKHMQANVRIPYGHQTNETFSSSFYFGPNHY
                     QTLKSYNIQLEKIVPLGWGIFGWVNKFLVIPVFNWLNTFNLNFGIIILILTIIIKVAL
                     LPLTYNAYKSGAKMRVLQPEIKEIQDKHKEEPLKLQQELMGLYKKAGVSPLGGCIPLL
                     LQMPILIAMFRFFPASIELRQESFLWATDLSTYDSIWNFGKLPIIDYIYGDHVSLFTL
                     LMTISTLLYTHFNMQMSSAMNAQMKWMQYIMPVMFLGIFNNYSAGLSYYYFLANMISI
                     GQQSLFRAFIDEDKIHAQIQENKKRPAANKKSAFQTRLEKMAKERGYQLPKK"
     gene            3082..>3229
                     /locus_tag="PLO59_02075"
     CDS             3082..>3229
                     /locus_tag="PLO59_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008243122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HQU99912.1"
                     /translation="MSEQNNPGNQLNIELTEDVAEGIYTNLAIITHSNSEFVVDFVKV
                     MPGVP"
BASE COUNT         1125 a          575 c          562 g          967 t
ORIGIN      
        1 taagaaaatt gacgaatgcg taacgccttt agcataaacc aattgcgaag aagcgtgaag
       61 cggaagaaaa ttatttcagc ttaaacatgt acaattactc ggtttgtgat agtatccata
      121 aaaaattatt cgcgagcaaa tgataaaaaa cattaagctt tacatgcaac tgctaaaact
      181 caaaatgaat tggttatttc atcactccta aactaaaaaa aatgaacttg aacaggaaaa
      241 caaaaagcat ttttattctt gcaattttat ttgcaaccat gacagttaaa gcgcaacaac
      301 ggattaaagg aagctttaac actgttacaa tgcttgtaat acctataaat ccaagcgatt
      361 ctgttttttt gcgaacttta aaaaacacag gttatatggc aggcttccct tcctttgtag
      421 ctgacccggg tgttgcaaat tatactaaag cagaaacgga tacaataatt cagaattttg
      481 aaaaagtaaa ccatcccgac aagcattttc aaacacaagg cgaatttgat ttatttctga
      541 acaaatggat gattgagctg aatgtgaaaa gaaataaaac gaaccgggaa acaaatattt
      601 cagatacatt aataattaaa aaatgaaata gctttttaat tttatttgtg ccgatgctta
      661 attaaacaca tacgatgaaa ctacttaccc aaatattgat atcgacattg gctattatgt
      721 ttaccaccta tttgttacca catgtacatg ttgatgcacc ttggcatgca tttttagttg
      781 caatattatt agccggactt aatatgctgg taaaacccat tttggttgtg tttactttac
      841 ccattaccat ttttacttta ggcttgtttc taattattat aaacggtatt atggtaatga
      901 tggttgatta tttaatggat ggctttaagg ttgacaattt actttgggcc ataattttta
      961 gtgttatttt aacaggaatt aataccattt tacaatcggt attaggaact aacaatcgtt
     1021 agttgctgaa ctaggttagt taatacaatt accaactata gctaacagca aaagttgtta
     1081 accagtattt aagcttttaa taataacaag cgcttacaat aggtgtacgt acgttacgtc
     1141 agtagcaagg cgaaactttt tatattcgcc cccctaaaat tttaaaacga tacgatggat
     1201 aggaattcga ttattggatt tgtgctgatt gcattggtac tggtaggtta cagctattac
     1261 acagcgccta ctaaggaaga gcttgcccgc aaacaaatgg agcgcgactc gttggcaatg
     1321 gttgcccaaa aacaggcatt agatagtatt aaactaacac aggttattac ggttaccgat
     1381 acggtgcagc taagcgattc ggccaaacaa cagcatttgg taaatcagtt aggcttgttt
     1441 gcaacggctg cccaaggcac aacacaacgg tttacgttag aaaacgaaaa acttataatt
     1501 acccttaata gccaaggtgg taaagttgag caggttgaat taaaaaacta caccacacac
     1561 gataaaaaac cactgctgct ttatcaagcc gactcatcgg caatgggttt aaacttcttt
     1621 gcacaaaaca aacaaatcag caccaatgca ctttatttta aacccttagg caaatctttt
     1681 aaagtttcgg gcaatggcag tcaatcgttt ataatgcgtt tagaagccac acccgaaaaa
     1741 tatattgatt acatatacac tttacaaggc aatagttttg atgtaaaaat ggatattacc
     1801 ttacatggtg tgcaagatgt aattgcagca aacagtaact accttgagct tgattggcga
     1861 gctgccttac tgcaacaaga acttaccatg aatgccgaac gcagtgtaag taccgtttac
     1921 tacaaataca cagatgataa agttgattat ttaagcgaaa gcgaaagcga gcaaaaatcg
     1981 ttaaatgaaa aaataaaatg ggtagcattt aaacaacagt atttttcgtc tatactcatt
     2041 gccaaaaatt tatttgaacc tgcaattgta gcagtacagg taaataaaaa cgataccgat
     2101 atagttaaac acatgcaagc caatgtgcgc ataccatacg gccatcaaac aaatgaaact
     2161 ttttcatcat cgttttattt tggtcccaat cattatcaaa ctttaaaatc atataacatt
     2221 cagttagaaa aaattgtacc cttaggttgg ggtatttttg gatgggtaaa taaatttttg
     2281 gtaatacccg tatttaactg gcttaatact tttaatctga actttggtat tatcattctc
     2341 atacttacca ttataattaa agttgcgcta ttgccgctaa cttacaatgc gtataagagt
     2401 ggcgctaaaa tgcgtgtatt gcagcccgaa ataaaagaga tacaggataa gcataaagaa
     2461 gaacctttaa agttacaaca agaacttatg ggcctttata aaaaagcagg tgtaagcccg
     2521 ttaggtggtt gtattccatt gctgttacaa atgcccatat taatagccat gttccggttt
     2581 tttccggcaa gtattgaact aaggcaagag tcgtttttat gggcaaccga cttatcaact
     2641 tacgacagta tttggaattt tggcaaacta cccatcattg attacatcta tggcgaccac
     2701 gtaagtttgt ttacattgtt gatgaccatt tcaaccttac tatacacaca ttttaatatg
     2761 caaatgtcat cggccatgaa tgcccaaatg aaatggatgc aatacattat gccggtaatg
     2821 tttttaggta tttttaataa ttactcggcc ggtttaagtt actactactt tttagccaat
     2881 atgatatcta ttggccagca aagtttgttc cgcgccttta ttgacgagga taaaatacat
     2941 gcgcaaatac aggagaataa aaaaagacct gctgccaata aaaagtcggc atttcaaacc
     3001 cggctcgaaa aaatggccaa agagcgcggt tatcagttac ccaaaaaata aaaccatagt
     3061 tttactttaa aatataatat catgtcagaa caaaataatc caggtaacca gctaaacatc
     3121 gaattaactg aagatgttgc cgaaggcatt tataccaacc tggctataat aacccattcg
     3181 aactcagagt ttgtagttga cttcgttaag gtaatgcccg gtgtaccaa
//
LOCUS       DAOMAA010000100         3227 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_22831_length_3227_cov_3.173077, whole genome shotgun sequence.
ACCESSION   DAOMAA010000100 DAOMAA010000000
VERSION     DAOMAA010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22831_length_3227_cov_3.173077"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1181
                     /locus_tag="PLO59_02080"
     CDS             <1..1181
                     /locus_tag="PLO59_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99913.1"
                     /translation="SKLQVLILALSLHTDISTIKDALSSSQNYDVSVNYIDDFKGNVT
                     SYNLIILHQLPDAKHKNLIDQIYKSDVPQWTIIGMQTDITALNGVQAGININASNGKT
                     NSVQASMDKNFGLFTWSVALQESIENWPPLTIPFGVYRPTSECVIALNQRIGNVVSNQ
                     PLLLLSNLNKKNAILCGEGLWRWSLKDFAVNNSKTLADEFILKTVQYLATAENKSPFR
                     VKHKNYVYDNEAIIIDAELVNEAGELINSNDVMLEISGNGKTYNYTFSKTNLAYTLQA
                     GLFPVGTYKLKSTASIGTKQYTYHGSLTVAPLQAELTSSIANHNMLLNVAQRSGGVMV
                     YPQSLDAISKLIESRPEITSTSYLHKELKDLVNEKWVFVLLITLLSTEWFVRKYAGAY
                     "
     gene            complement(1178..1894)
                     /locus_tag="PLO59_02085"
     CDS             complement(1178..1894)
                     /locus_tag="PLO59_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99914.1"
                     /translation="MQLIMRSFNKLLLCLATIAAFFNFQGCTKSNIPAIDFGYNYFPV
                     NINHYIIYDVDSVNKDAFTGLVTHSSYQIKEVIAGVYNDNEGRPTLRIERFRKDTSTY
                     IDWTIFQVWTANLTTTTAQRFEDNKRYVKLTFPVKENRVWNGNSMNTEDAEDYTYQNV
                     NQPEYFNQLVFDSVLTVLQADDLSNIISPKYKLEKYASGVGLVYRKKFEVTMHTNPIT
                     GNLDTSAFVDYTESILSFGN"
     gene            complement(1875..3008)
                     /gene="mnmA"
                     /locus_tag="PLO59_02090"
     CDS             complement(1875..3008)
                     /gene="mnmA"
                     /locus_tag="PLO59_02090"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HQU99915.1"
                     /translation="MSKHGKILVAMSGGIDSSVTAMLLHDAGYEVIGITMKTWDYASS
                     GTTGKKETGCCSLDSINDARSIAVDKGFPHYILDIRNEFGDFVIDNFVSEYLGGRTPN
                     PCVLCNTHIKWEALIKRADMLDCEFIATGHYAQTRQENNRYIVSKGVDTAKDQSYVLW
                     GLTQESLARTKFPIGGFKKTEIRQMALDMGFEELSKKSESYEICFVPDNDYRAFLKHR
                     VPGIETKLGNGNFIDMDGKVMGKHNGYPFYTVGQRKGLEIAFGQPMYVIKINPNDNTV
                     VLGPYEALQKQTMLVRDVNLIKFESLIEPVEALTKIRYKDPGTHATLIQQDNLMRVDF
                     HKPVNAVAPGQSAVFYDDMDVIGGGFIARDNAVEKIKHATNHA"
     gene            complement(3059..3220)
                     /locus_tag="PLO59_02095"
     CDS             complement(3059..3220)
                     /locus_tag="PLO59_02095"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HQU99916.1"
                     /translation="MGVCGGIADYFNMDVVVVRLIAVGLLLLGVGSPVIIYIIMAIVV
                     PDENHIINN"
BASE COUNT         1041 a          622 c          536 g         1028 t
ORIGIN      
        1 aaagcaaatt acaggttttg atacttgcgc tatcactaca cacagatata agtacaatta
       61 aagatgcatt aagcagtagt cagaattatg atgtaagcgt aaattatatt gatgatttta
      121 aaggaaatgt aaccagttac aatttaataa tattacatca gttgcctgat gcgaaacata
      181 aaaatttgat tgatcaaatt tataaaagcg atgtaccaca gtggactatt attggcatgc
      241 aaaccgatat aactgcctta aatggagtgc aggcaggtat aaatattaat gcttccaacg
      301 gtaaaacaaa ttcagtacaa gccagtatgg ataaaaattt tggcctcttt acatggagtg
      361 tggccttgca agaatctatt gaaaactggc cacccttaac catacctttt ggcgtttaca
      421 ggccaacatc ggaatgtgtt attgcattaa accagcgtat aggtaatgtg gtaagtaatc
      481 agcctctttt attgttgagt aacttaaata aaaaaaatgc aatactatgt ggcgaaggct
      541 tatggcgctg gagtttaaaa gactttgcag taaacaactc gaaaacatta gcagatgaat
      601 ttattcttaa aaccgtgcag tatttagcaa cagccgaaaa caaatcgcca ttcagagtaa
      661 agcataaaaa ttatgtttac gataatgaag caattattat agatgctgaa cttgttaatg
      721 aagctggtga attaataaac agtaatgacg taatgcttga aatttcgggt aatggaaaaa
      781 catataatta tacttttagt aaaaccaatt tggcttatac gttgcaagcc ggcttgtttc
      841 cggtaggtac ttataaacta aaatcaactg cctcaattgg caccaaacaa tacacatatc
      901 atggttcgtt aaccgttgca ccgttacaag cagagttaac ttcaagtatt gccaaccata
      961 atatgttact taatgttgcg caacgcagtg gcggtgttat ggtttatccg caatcattag
     1021 acgcgataag caaattaatt gaaagcaggc ccgaaataac cagtaccagt tacttgcata
     1081 aagagcttaa agaccttgtt aatgaaaaat gggtttttgt tttacttatt acgttgctta
     1141 gtactgagtg gtttgtaaga aaatatgcag gtgcatatta attcccaaaa cttaaaatgg
     1201 attcggtata atcaacgaat gcactcgtat caagattgcc tgtaatggga ttggtgtgca
     1261 tggtaacttc aaacttcttt cgataaacca aacctacacc actggcatat ttttcgagct
     1321 tgtatttagg cgaaattata ttggataaat catctgcttg taaaacagtt agtacactat
     1381 caaaaacaag ttgattaaag tattcaggtt gatttacatt ttgatatgtg tagtcttctg
     1441 catcttctgt attcatactg tttccattcc aaacacggtt ttcttttacc ggaaaagtca
     1501 acttaacata gcgcttatta tcttcgaaac gttgtgctgt agttgtggtt aggtttgctg
     1561 tccaaacctg aaaaatggtc caatcaatat aagtcgaagt atcttttcta aaacgctcaa
     1621 tgcgaagcgt tggtctgcct tcattatcat tgtaaacacc tgcaattact tctttaatct
     1681 gataactact gtgggtaact aaaccagtaa acgcatcttt gtttaccgag tcaacatcgt
     1741 aaataatgta atgattaatg ttaaccggaa agtagttgta accaaaatca attgcaggta
     1801 tattgctttt agtacaaccc tgaaaattga aaaaagcagc aatagttgct aaacataata
     1861 gcagcttatt aaaactacgc atgattagtt gcatgtttaa ttttttcaac agcattatcg
     1921 cgggcaataa atccaccacc aataacatcc atatcatcat aaaacacagc actttgaccg
     1981 ggtgcaactg catttacagg tttatgaaaa tcaacacgca tcaggttgtc ttgctgaatt
     2041 aatgttgcat gtgtgcccgg atctttatat ctgatttttg ttagtgcttc aactggttca
     2101 ataagtgact cgaattttat taaattaaca tcacgtacca gcatggtttg tttttgcaaa
     2161 gcttcgtatg ggcccaaaac taccgtgttg tcgtttggat ttattttaat aacatacatg
     2221 ggttggccaa aggctatttc aagccctttg cgttgcccca cagtataaaa cggataccca
     2281 ttatgcttac ccataacttt accatccata tcaataaaat taccattacc cagtttggtt
     2341 tctataccgg gcactctgtg ctttaaaaaa gcacgataat cgttatcagg cacaaagcaa
     2401 atttcataac tctcactttt tttcgataac tcttcaaatc ccatatctaa tgccatttgc
     2461 ctgatttcgg ttttcttaaa acctccgata gggaatttag tgcgggctaa actttcttgt
     2521 gtcaagcccc atagcacata actctgatct ttggcagtat caactccttt gctaacaatg
     2581 tatctgttgt tttcttgtct ggtttgcgca taatgcccgg tggcaataaa ttcacaatct
     2641 aacatatcgg ctcttttaat caatgcctcc catttaatat gtgtgttaca taatacgcat
     2701 ggatttggcg tgcgaccacc taagtattct gaaacaaagt tatcaattac aaaatcgcca
     2761 aattcatttc tgatgtctaa aatataatgc gggaaacctt tatcaacagc aatacttctt
     2821 gcatcattta tcgaatctaa actacaacaa cctgtttctt ttttaccggt agttcctgat
     2881 gaggcataat cccaggtttt catggttata cctataactt cataacctgc atcgtgtaat
     2941 aacatggccg ttaccgaact atcaatgccc ccactcattg ctactaaaat ttttccgtgc
     3001 ttactcattt catcaaaatt aatttaatac gcttgctgcc cccttcattc aatcaaactt
     3061 aattatttat tatatggttt tcgtcaggta ccactatggc cattataatg taaattataa
     3121 ccgggcttcc aacaccaagt aataaaaggc ctactgcaat aagccttact accactacat
     3181 ccatattaaa ataatcggct atgccaccgc aaacgcccat tagcatt
//
LOCUS       DAOMAA010000101         3191 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_23313_length_3191_cov_3.388393, whole genome shotgun sequence.
ACCESSION   DAOMAA010000101 DAOMAA010000000
VERSION     DAOMAA010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23313_length_3191_cov_3.388393"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1373
                     /locus_tag="PLO59_02100"
     CDS             <1..1373
                     /locus_tag="PLO59_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013620824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HQU99917.1"
                     /translation="ADTVTVLENIGHFLDEEVELNFKKFKQEEYSNTQISEMIAAHLD
                     VTRVLRRASKNWDGGYVICGLIGHGDAFVLRDPSGIRPAYYYADDEVVVVASERPAMQ
                     TAFNIQVDKIEEINPGCALIIKQNGQWSVQKVREPLPKKSCSFERIYFSRGSDETIYK
                     ERKELGRRVVPSILEAVNFDLKNTVFSFIPNTAETAFYGMIKGVEEYINQSKVRKILE
                     SNKKLSPDALTEIINLRPRVEKIAIKDAKLRTFITDDAQRDDLVAHVYDVTYGTINRG
                     IDNLVIIDDSIVRGTTLKKSILRMLDRLGPKKIVIVSSAPQLRYPDCYGIDMAKLGDF
                     VAFNAAIQLLEENNMEHIIDEVYHLCKEQLTLPKESMVNHVQKIYEPFTDEQISKKIG
                     QLLTPKNINAEVEIIYQRVEDLHKACPNHSGDWYFTGNFPTAGGMKVSCRAFVNYVEG
                     KNERAY"
     gene            complement(1410..>3191)
                     /locus_tag="PLO59_02105"
     CDS             complement(1410..>3191)
                     /locus_tag="PLO59_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQU99918.1"
                     /translation="SLSSTGGPVSMLNNNVLDKSDFITGAFPAQYGNALSSVFDLRMR
                     NGNTQQHEFLAQVGFNGFEANAEGPLSANHKASYLASYRYSTLGAFSAIGINFGTGTA
                     TPAYQDGSFKFFIPLSEKTKLSVFGMGGGSGVDFYGNDVDTTITDLYGDENVNVKVKY
                     LTLIGGLELETNFNTKAYGKFFAGISTTHENYDGDSISVITREAYPNGIAQFETQKYT
                     AGYQFAYKFNAKNSLLTGFNTDAIKFDLFNAYIKYDSLINPTQITLVDNNDVTLLTQA
                     FMQLKHRFTENFSAVAGLHAQQLTLNNDFALEPRLGLKYMFNNKQALSLGYGLHSQMQ
                     NIYTYYVETTVGTQVMQTNKSLGFTKAHHFVLSYDYTINDKLRLKAETYYQDLFDAPV
                     TQNPSSFSGLNTGNNFGPSDEDSLVNNGTGTNYGVELTVEKFYGKGYYFLTTVSVFDS
                     KYKGSDGIKRNTAFNTGYALNVLGGKEFKLKDKSKIIALDIKVSTTGGRYTTPLDTTA
                     SAQEGRAVYKEDEAYSVRQSAYFRVDAKITYRKNYAKSTLEFSLDLQNVTNHQNIFYQ
                     SYNPRTNEVVNEYQQGFFPVPTVRFTF"
BASE COUNT         1000 a          620 c          614 g          957 t
ORIGIN      
        1 aagccgatac cgtaaccgta ttagaaaaca tcggacattt tttagacgaa gaagtagaac
       61 tgaattttaa aaaatttaag caggaggaat atagtaatac gcaaatttcg gaaatgatag
      121 ctgcccattt agatgttaca cgcgtattaa ggcgggcatc aaaaaattgg gatggcggtt
      181 atgttatttg cggattaatt ggccatggcg atgcatttgt attacgcgac cccagtggca
      241 taaggccggc atactattat gccgatgacg aagtggtggt agtagccagt gagcgccctg
      301 caatgcaaac agcttttaat attcaggttg ataagattga agaaataaac ccgggttgtg
      361 ccctgattat aaaacaaaac ggacaatggt ctgtacaaaa agtacgcgaa ccattgccta
      421 aaaaatcgtg ctcgtttgag cgcatttact tttcgcgtgg cagcgatgaa accatttaca
      481 aagaacgtaa agaattaggc agacgggtgg taccttctat acttgaagcc gtaaattttg
      541 atttaaaaaa caccgtattt tcgtttatac ccaacacagc cgaaactgct ttttacggta
      601 tgataaaagg tgttgaagaa tacattaacc aatcgaaagt gcgcaagatt ttagaaagca
      661 ataaaaagct aagtcccgat gcacttaccg aaattattaa cctgcggccg cgcgttgaaa
      721 aaattgccat taaggatgcc aaactgcgta catttattac cgatgatgca cagcgcgatg
      781 atttggtagc gcatgtgtat gatgttacct acggcactat aaaccgcggt atagataacc
      841 tggtaattat tgacgattcg atagtacgcg gtactacctt aaaaaaatca atactacgta
      901 tgttggatag gttagggcct aaaaaaattg taattgtatc atcggcacca caattacgtt
      961 accccgattg ttatggtatt gacatggcaa aactgggcga ctttgttgca tttaatgcag
     1021 ccatacaatt gctcgaagaa aacaatatgg agcatattat tgatgaggtt tatcacctgt
     1081 gcaaggagca attaaccttg cctaaagaaa gcatggtaaa ccacgttcag aaaatatatg
     1141 aaccctttac cgatgaacag atttcgaaaa aaatagggca attgctaaca cctaaaaaca
     1201 tcaatgccga agttgaaatt atttaccagc gtgttgagga cttgcataaa gcctgtccca
     1261 atcactcagg cgattggtat tttaccggca attttccaac ggccggtggt atgaaggtaa
     1321 gttgccgtgc ttttgtaaat tatgttgaag gaaaaaacga acgtgcttat tgataacggg
     1381 cacgttatac gccaagtgca acagcattgc taaaatgtaa aacgcaccgt tggcaccgga
     1441 aaaaatcctt gctgatactc gttaactact tcgttagtac gcggattgta cgactggtaa
     1501 aaaatgtttt gatgattggt tacattttgc aaatcgagcg aaaactctaa agttgatttt
     1561 gcataatttt tccggtaggt aatttttgca tccactctaa aataagccga ttgccgcacg
     1621 ctataagcct catcttcttt atacactgca cggccttcct gtgccgatgc agttgtatct
     1681 aatggtgttg tgtaacggcc tccggtagta ctcaccttaa tatctaatgc aataattttt
     1741 gatttgtcct ttaatttaaa ctccttacca cccaatacat ttaaagcata acctgtatta
     1801 aatgccgtat tacgtttaat cccatcgctg cctttatatt tcgaatcgaa taccgatacc
     1861 gtggttaaaa agtaataacc tttgccataa aatttttcaa ccgtcaactc aacgccatag
     1921 tttgtgccgg taccattatt taccaagcta tcttcgtcag atgggccaaa attattaccg
     1981 gtatttaatc cgctaaacga tgacgggttt tgtgttaccg gtgcgtcaaa caaatcttga
     2041 taataggttt cggcttttaa acgcaactta tcatttatgg tgtaatcata actcaaaaca
     2101 aaatgatggg ctttggtaaa acctaagctc ttattggttt gcattacttg tgtacctact
     2161 gtggtttcaa catagtaagt gtatatgttt tgcatttggc tgtgtaaacc ataacccaaa
     2221 cttaaagcct gtttattatt aaacatatac tttaagccca aacgcggttc caatgcaaag
     2281 tcattattta atgtgagttg ttgcgcatgc aaacctgcaa ctgcactaaa gttttcggta
     2341 aaccgatgtt ttaattgcat aaaggcttgt gttagtaagg ttacatcatt gttatcaacc
     2401 aaggttattt gtgttgggtt aatcaaacta tcatatttaa tgtaggcatt aaacaaatca
     2461 aacttaatag catcggtatt aaagccggta agcaaactgt ttttagcatt aaacttatag
     2521 gcaaactgat agcccgctgt atatttttgt gtttcgaatt gtgcaatacc attgggatat
     2581 gcttcgcgtg ttattacact tatgctatcg ccatcataat tttcatgagt agtactaatg
     2641 ccggcaaaaa atttaccata cgcttttgta ttaaaatttg tttctaattc gaggccacct
     2701 attagtgtga ggtactttac ttttacgttt acattttcat caccatacaa atccgtaata
     2761 gtggtatcca catcgttgcc ataaaaatca acaccgctgc caccacccat accaaaaacc
     2821 gacagttttg ttttttcact tagcggtata aaaaatttaa acgaaccatc ctgataagca
     2881 ggtgtggcag tgccggtgcc aaaatttata cctatggcac taaatgcacc caatgttgag
     2941 taacgataac ttgccagata agatgcttta tgattagctg ataaaggccc ttcggcattg
     3001 gcttcgaaac cattgaaacc cacttgtgct aaaaactcat gttgttgtgt gtttccatta
     3061 cgcatacgca aatcgaaaac acttgataat gcattgccat attgtgccgg aaatgcaccg
     3121 gtaataaaat ccgatttatc gagcacatta ttattaagca tacttaccgg gccaccggta
     3181 ctgcttaacg a
//
LOCUS       DAOMAA010000102         3185 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_23396_length_3185_cov_3.062620, whole genome shotgun sequence.
ACCESSION   DAOMAA010000102 DAOMAA010000000
VERSION     DAOMAA010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23396_length_3185_cov_3.062620"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1573)
                     /locus_tag="PLO59_02110"
     CDS             complement(<1..1573)
                     /locus_tag="PLO59_02110"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HQU99919.1"
                     /translation="MKLTNKFLDKKNFELADSSSFKNIAQLNKHYYETGNSHSMRFYA
                     LAGLNKVQQFLPLASSDANKKVLRQFEMLFNRQAGISYFEDGDYTNQLKYFQQFLVAA
                     QKLNSINDIATAYTYIATCHRELEDYHKSFELAKKAVYTLSGCNYPATLATAYALISN
                     YYLDEKDNTDSVYYYRKKAYLLFKETNNTMLLASSAFDLVEFFHHIGQTDSCKKYLAE
                     VEHLIAELQNPEQTIIYNCYMAQVELMEGNTNQAVTLLQKARSLADETIELNDNSQIN
                     RNLALALAAAGKTGDAFKVLESAFNEYSADLNTDKVRSLTQAQMNFDFEKERAIAAIE
                     LKREKQQRNFLIVLASVLLLFAGFIFQRYRERHRTIIMLAEKNETIEKAYSDLKEAQQ
                     ALIDTEKQREAQNIRVRIARDIHDEIGSGLTKITLLSDVAKKKTQQTDVANALSKITS
                     FSKNVSTSLSEIVWAINPGNDTVDSLINYLKSTAANLLDDSGLNYTLHFPLTESGASI
                     HPEIKRNIYLVMKEAL"
     gene            complement(1790..2824)
                     /locus_tag="PLO59_02115"
     CDS             complement(1790..2824)
                     /locus_tag="PLO59_02115"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HQU99920.1"
                     /translation="MKRKLRLKPYLHILKMEQLVHLIKTLDEGVQLPNGTFFNVVLQC
                     FSEFTYEDEIVDALKTHLSADDVVVNQLLNKLFDLAVIEFFDDELPEALNRYHRQLLL
                     FDAVNPKNNFEENYGCQSKLRQTHVLVLGIGGIGNFIATSLAASGVGHITLVDFDVVE
                     ISNLNRQILFTENDLGNSKVHAAANRLKAINQQCIINAIEKEIHSLQAFEKLLKSIDK
                     IDYVVLSADKPVDMVLWASALCKPYKFKYLKCGYMAYQGLIGPLLGYNTKPYDEIYQS
                     WAVDIQSQDALIHAQNEKHIAPSMAATNAILANIATWELLKDITGICESVLIEQRILF
                     NLKTMEMRFG"
     gene            complement(2891..3061)
                     /locus_tag="PLO59_02120"
     CDS             complement(2891..3061)
                     /locus_tag="PLO59_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99921.1"
                     /translation="MLSETLIIFINAILAINAPEQTIAPVEKISAVKTEQTTTPTTPP
                     ITERRRGGWDGN"
BASE COUNT          923 a          594 c          563 g         1105 t
ORIGIN      
        1 ttaaggcttc tttcattacc aagtaaatat tgcgttttat ttcgggatgg attgaagccc
       61 ccgattcggt tagcggaaaa tggagtgtgt aatttaatcc ggaatcgtca agcaaatttg
      121 cggcagtaga tttcaaataa tttataaggc tatctactgt atcattgccg ggatttattg
      181 cccatacaat ttcgcttaat gatgtactga cattttttga aaaggatgtg atttttgata
      241 atgcattggc tacatcagtc tgttgcgttt tcttttttgc cacatcactt aataaagtaa
      301 tttttgtaag gccgctgcca atttcatcat gtatgtcgcg tgcaatgcgt acccgaatgt
      361 tttgtgcttc gcgttgcttt tcggtgtcta tcaatgcttg ctgcgcttct tttaaatcgc
      421 tgtaagcctt ttcaattgtt tcattttttt cggcaagcat tataatggta cggtgccttt
      481 ccctataacg ctgaaaaata aatcctgcaa agagcaaaag taccgaagct aatacgatga
      541 gaaagttgcg ctgctgcttc tcacgtttta attcaattgc agcaatagct cgctctttct
      601 caaaatcaaa attcatttgt gcttgtgtta agctgcgcac tttatctgtg ttaaggtctg
      661 cactgtattc gttaaaggcc gactctaaaa ctttaaaagc atcgcctgtt ttgcccgcag
      721 cagccaatgc caatgccaaa tttcggttaa tctggctgtt gtcgttcaat tcaattgttt
      781 catcagcaag gctacgggct ttctgcaata gtgttacagc ttggttagtg tttccttcca
      841 tcaattcgac ttgcgccatg taacagttat aaattatagt ttgctcagga ttttgtaatt
      901 cagctattag gtgttccact tcagctaaat actttttaca ggaatcagtt tgcccgatgt
      961 gatgaaaaaa ttcaaccaaa tcaaaagctg aacttgccag caacatcgta ttgtttgttt
     1021 ctttaaacaa gagatatgct tttttacgat aataataaac gctgtcagta ttatcttttt
     1081 catcaagata ataattgctt ataagtgcat acgcagtagc taatgttgcc ggataattac
     1141 aaccgcttaa agtataaaca gccttttttg ctaactcaaa tgacttatga tagtcttcta
     1201 attcgcgatg acaagttgct atatacgtat aagctgttgc aatatcgttt atgctgttaa
     1261 gcttttgtgc tgctactaaa aattgctgaa aatatttgag ctggtttgtg taatcgccat
     1321 cttcgaaata ggatatacca gcctgcctgt taaatagcat ttcgaactga cgaagcactt
     1381 ttttatttgc atcgctcgaa gctaatggca aaaattgttg caccttgtta agtcctgcca
     1441 atgcataaaa acgcatagaa tgggagttgc ccgtttcata ataatgcttg ttgagttgtg
     1501 caatgttttt aaaactacta ctatccgcta attcaaagtt ttttttatcc agaaatttat
     1561 tggtcaattt caaaaggctt tcgctgttaa tatcctgcgc atttacactg caccctatat
     1621 agctgaaaca aatgattacg tgaatcaaaa aacgattata actactactc attatttatt
     1681 tttcaattat cgaataaagc acattgaaaa ggtattgcag ttaaaatatt ggaattaaat
     1741 tttgtttcat ttttaaaata caaaccccat aaaattacgt gctgttaaat tatccaaaac
     1801 gcatttccat ggtttttaaa ttaaacaata tgcgttgttc aattagtacc gattcacaaa
     1861 tacctgttat atcttttagc agctcccagg ttgcaatgtt ggcaagtatt gcattggtag
     1921 cggccattga aggcgcaata tgtttttcgt tttgagcatg tattaacgca tcttgtgatt
     1981 ggatatcaac agcccaactt tgataaattt catcataggg cttggtgttg taacctaaca
     2041 atgggcctat caatccctga taggccatat aaccacactt taggtattta aatttatagg
     2101 gcttacatag tgctgatgcc cataaaacca tatccaccgg tttatcggcc gataatacta
     2161 catagtcgat tttatctatt gattttagca gcttttcaaa tgcttgtaag gaatgaattt
     2221 ctttttcaat cgcatttata atgcattgtt gatttatagc ttttaaacga tttgcagcag
     2281 catgtacttt gctattaccc aagtcgtttt cggtaaaaag tatttgcctg ttcaggttcg
     2341 atatttcaac tacatcaaaa tcaaccaatg taatgtgacc aacacccgaa gccgctaacg
     2401 aagttgcaat aaaattacca atgccgccaa tgcctaacac aagcacatgc gtttgccgta
     2461 gtttactttg gcagccgtaa ttttcttcaa aattattttt agggttaacg gcatcaaata
     2521 gtagcaactg acgatgataa cggtttaacg cttctggcaa ttcgtcatca aaaaactcta
     2581 ttacagctaa atcaaagagt ttattgagca attgattaac cacaacatcg tcagcactta
     2641 aatgggtttt aagtgcatcc actatttcat cttcgtaagt aaactcacta aaacattgca
     2701 gtacgacatt aaaaaaagta ccattgggca attgcactcc ttcatctaaa gtttttatca
     2761 gatgcactaa ttgctccatt tttaaaatgt gcaagtaggg cttaagtcta agttttcttt
     2821 tcacaaatag aaattatgaa gcaaaatagc aacaggagtt ttgcaaacaa agcaaattta
     2881 atagctttac ttagttgcca tcccaaccgc cacgcctgcg ttcggttata ggtggtgttg
     2941 ttggtgttgt agtttgttct gttttaactg cacttatttt ttcaaccggt gcaatagttt
     3001 gctcaggtgc atttatagcc agtatggcat tgatgaaaat aattaatgtt tcagataaca
     3061 tagcatgaag ttttaaggtt tgattaattt ctatcccaac cgccacgcct gcgttcggtt
     3121 ataggtggtg ttggtgttgt agtttgttct gttttaactg cacttgtttt ttcaaccggt
     3181 gcaat
//
LOCUS       DAOMAA010000103         3184 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_23404_length_3184_cov_2.506552, whole genome shotgun sequence.
ACCESSION   DAOMAA010000103 DAOMAA010000000
VERSION     DAOMAA010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23404_length_3184_cov_2.506552"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="PLO59_02125"
     CDS             complement(<1..405)
                     /locus_tag="PLO59_02125"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HQU99922.1"
                     /translation="MILKKIIALIIAGTPFFALGQYTISGKITDTKNEPLPGAAIALL
                     NPNDSSVTTGTVTNLDGDFKIEKIKSQLYLIKVSYLGFGDFYKKQFINADLNLGNITL
                     SDKAALLKDAQVVGDVLPAKLIGDTMQFNADAY"
     gene            complement(617..1426)
                     /locus_tag="PLO59_02130"
     CDS             complement(617..1426)
                     /locus_tag="PLO59_02130"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HQU99923.1"
                     /translation="MENPRFLIATDLSDAASKATDYAIEFGKRFNASLLLLHIFEVAN
                     VDANSNKLMSANFLNREIKKQIQGTVNDIESKHGLQANYMSKEGDLFVLLGEAASETH
                     SDIMFIGTHGIRGVQHIIGSFIAKVINGSALPILVVQKESKPCALKSIAIWIDHSQQN
                     PIEGWIIAIAKSFQTHIHFVFSEDAVGPGVNAHVDIINQSMQQHQLPYTVYHINEIAD
                     KHTHFLNYASSIDASLLVCCRHGHLVNKPIEEIITNKHHIPVLCLYVDGKA"
     gene            complement(1481..2383)
                     /locus_tag="PLO59_02135"
     CDS             complement(1481..2383)
                     /locus_tag="PLO59_02135"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99924.1"
                     /translation="MWVKQHQLLGHSGAIYAIGKGYLDNTILTGGADQVLVQWHTETA
                     AAGKVIAKAAGTIYCIKVIDALPYILMGTATGDLHIINYAQNKEVKLLRYHQNGVYDV
                     VAYQPLQLMLIAGGDGLLSVCNIEGQLIKQVKIAPHKLRYIAIHPAQDVAYIGCGNGH
                     LVSFDLQHLQVIADLPIHRTNWSCNTIAFINDNQIITGGRDAMLHITDVQSMQIQKSI
                     AAHNYAIYDMVFNADKSLMATASRDKSIKIWDTQTWHVLHKLSLDKTEGHKNSVNKIY
                     WHTGLNKIISVSDDKSIIIWQQQL"
     gene            2536..3183
                     /locus_tag="PLO59_02140"
     CDS             2536..3183
                     /locus_tag="PLO59_02140"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HQU99925.1"
                     /translation="MIKKTLFSFLVPILLLAIMWFIKLYEISMGIDLGVHGVLPRTLS
                     GLQGILFSPFLHGSINHLLSNTLPFLLLMAALIYFYEDIWLQSFIAMFLLSGIGLWCG
                     GRDSYHIGASGIIYALTGFLFTEGILRREVKYMAISLLVVFMYGGTIWAMMPMFKTIS
                     WEAHLFGFMAGVLMAIVYKNQGPKKTLYQWEKDEVDEMLGDKTEINYTFIPNSEK"
BASE COUNT          936 a          560 c          608 g         1080 t
ORIGIN      
        1 atatgcatcg gcattaaatt gcatggtatc gccaataagt ttggcaggca aaacatcacc
       61 caccacttgc gcatctttaa gcaaagctgc tttatcgctt agtgtaatgt ttcctaaatt
      121 taaatcagca tttataaatt gctttttata aaagtcgcca aagcctaaat aggaaacttt
      181 aatcagataa agttggctct ttattttttc gattttaaag tcgccatcta agttggtaac
      241 tgttccggtg gtaaccgaac tgtcgtttgg gtttagtaat gcaattgcag caccgggtag
      301 cggttcgttt tttgtatccg ttattttacc cgaaatggtg tattgcccta atgcaaaaaa
      361 aggtgtgccg gcaattatta aagcaattat tttttttaat atcatatatt gaaacagctt
      421 aagccaatta gagttgaaac caagctgcaa gtttaatgtt atgtaatgga tttacacaga
      481 aacaaaaagt ctttgcagcc ctttgtttta acggctttac cgattttgaa acttattaac
      541 ggctgttaat gtttgtttaa tttaaaactt gagtaaacaa acttacaaat caggatataa
      601 aacttggttg taatgcttag gctttgccat caacatacaa acacaaaacc ggtatgtggt
      661 gtttgttggt aattatttct tcgatgggct tatttaccaa atggccatgc ctgcaacaaa
      721 ctaataatga ggcgtcaata cttgaagcat aatttaaaaa atgtgtgtgc ttatcggcaa
      781 tttcattaat gtgatatacg gtgtaaggta attgatgctg ttgcatactt tggttaataa
      841 tatctacatg cgcattaact ccggggccta cagcatcttc tgaaaataca aagtgtatat
      901 gtgtttgaaa cgatttagca atggcaatta tccagccttc gattgggttt tgctggctgt
      961 ggtcaatcca aatagctata ctttttaggg cacatggttt cgattctttt tgtaccacta
     1021 aaataggtaa tgccgaacca ttaataactt tggcaataaa actacctata atatgttgta
     1081 caccgcgtat gccatgtgtg ccaataaaca taatgtcgga atgggtttcg gaagccgctt
     1141 cacctaacag cacaaataag tcgccttctt tcgacatgta gttagcttgc aaaccatgtt
     1201 tactttcaat gtcgtttacg gtgccttgaa tttgcttttt tatttcacgg tttaaaaaat
     1261 tagccgacat cagtttgttg ctgttggcat caacattggc aacttcgaat atgtgtagca
     1321 aaagcagcga tgcattaaat cgtttgccaa actcaatagc atagtcagta gcttttgatg
     1381 cggcatcact taaatcggta gcaattaaaa aacgtgggtt ttccatgtgg ttggctttgg
     1441 tagcaatgat aaataaaatc tttttgtatt actaagtgta ctatagttgt tgttgccaaa
     1501 taattattga cttatcgtca cttaccgaaa taattttgtt taaaccggta tgccagtata
     1561 ttttatttac tgagttttta tgcccctcgg ttttatccaa gcttagttta tgcaacacat
     1621 gccaggtttg tgtgtcccaa attttaattg atttatcgcg gcttgcagtg gccattaagc
     1681 ttttatcggc attaaaaacc atatcgtaaa tagcataatt atgcgcagca attgattttt
     1741 gtatttgcat actttgtaca tctgtgatgt gcagcatggc atcgcgccca ccagtaatta
     1801 tttggttatc atttataaaa gcaatggtgt tgcaactcca gttggtacga tgaatgggta
     1861 aatcggctat tacttgcaag tgctgcaaat caaaacttac caggtgtcca ttgccacaac
     1921 caatatatgc aacatcttgt gcaggatgta tggctatgta cctgagttta tgcggggcta
     1981 tttttacttg cttaattaat tgaccttcga tattacaaac agaaagtaaa ccatcgccac
     2041 cggctattag cattaactgc aatggttgat acgcaacaac atcataaact ccattctgat
     2101 gatatcgcaa taacttaacc tctttgtttt gcgcatagtt tattatatgt aaatcgcctg
     2161 tggcggtacc cattaaaatg taaggcagtg cgtcaataac tttaatacaa taaatagtac
     2221 cggctgcttt ggctataact ttgcctgccg ctgcagtttc agtatgccat tgcactaata
     2281 cctgatcggc accacctgta agtatggtgt tatccaaata tcctttacca attgcataaa
     2341 tagctccact gtgaccaagc aattgatgtt gtttaaccca cataaagaaa atagttgagt
     2401 ttaatttgaa gcaatattac aatcattgta attacggtac taatacattt atacgaatat
     2461 gcagttagtg cattgatgtt aaaatttatt acgtttaagc acgtcaaaaa aaatatgttt
     2521 tcggccgtaa gttaaatgat aaagaaaact ttgtttagtt ttttagtgcc cattctcttg
     2581 ttagccatca tgtggtttat taagttatat gaaatttcga tgggtattga cctgggtgtg
     2641 catggcgttt taccacgcac attaagtggt ttgcagggca ttttgttttc gccattctta
     2701 cacggcagca tcaatcattt attaagtaat acgttgccgt ttttgttact gatggcagcg
     2761 ctcatttatt tttacgaaga catttggctt caatcgttta ttgctatgtt tttgctttcg
     2821 ggcattggtt tatggtgcgg tggccgcgat agttatcata ttggtgccag cggcattata
     2881 tatgccctta ccggtttttt atttaccgaa ggtattttaa ggcgcgaagt aaaatacatg
     2941 gccataagtt tgttggtagt atttatgtat ggcggcacaa tatgggccat gatgcccatg
     3001 tttaaaacca tatcgtggga agcgcattta tttggtttta tggcaggtgt attaatggcc
     3061 attgtgtata aaaatcaagg ccccaaaaaa acattatacc agtgggaaaa agatgaagtg
     3121 gacgaaatgc tgggcgataa aactgaaatc aattacacat ttattcccaa ttccgaaaaa
     3181 taaa
//
LOCUS       DAOMAA010000104         3182 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_23442_length_3182_cov_2.832747, whole genome shotgun sequence.
ACCESSION   DAOMAA010000104 DAOMAA010000000
VERSION     DAOMAA010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23442_length_3182_cov_2.832747"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="PLO59_02145"
     CDS             <1..702
                     /locus_tag="PLO59_02145"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HQU99926.1"
                     /translation="DAAKRNAEESLIRERAHAEVMRVTAVMETREQERNRIAKELHDD
                     LGSGLTEIKLLCEIYKKNGINSNFEIEKIATSSGALIDKLSEIVWATNPRHDTLSSLL
                     VYIRQYAFNFLDNANIKCHANTIDVPAVPLTSEFRRNVFLTIKEALNNVVKYASTDKV
                     EIKMEIKLPENGNPNALLLIHIIDFGKGFQNQKLNETTNGLYNMKERMEGISGKFEVD
                     LTKGVHIKLTAPLLL"
     gene            789..1631
                     /locus_tag="PLO59_02150"
     CDS             789..1631
                     /locus_tag="PLO59_02150"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HQU99927.1"
                     /translation="MLHIETAKTILVPTDFTHVGDSALKHANIFAARTGKSISLLHVI
                     ANETEKAKALEKLDVIAIGNTKATNIETISMVSVGTIFDQIGAVADQVNAAFVVMGTH
                     GIIGMQKVTGSKALKVITKSHVPFIVVQKPPAHSTYLNFVIPISYQRESKQPIFNIAE
                     LARIFGSTCHLVYASINDEQLEMNIKNNVAYAESFFDEHNIKFVTHKLDAKFAQFNEA
                     CLKYAKQIEADLITLITEQNISMAEFLMRPAEQFFIANELAIPVMCIHPDYKAVQYGS
                     VFAN"
     gene            1646..2488
                     /locus_tag="PLO59_02155"
     CDS             1646..2488
                     /locus_tag="PLO59_02155"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HQU99928.1"
                     /translation="MNNNDFSKTILVPYDFTVKADAAIKHANSFAKKLSKTVTLLHVI
                     ADYDEKTETLARLKAIAANNQKITDITTEYCTSTGTIYDQIGQAADILDAAFVVMATH
                     GINGVQKVFGSKALKVITKSHKPYIVVQDEPADQLYKQIIVPMSYQPECKQPLFHIMN
                     IAKLFNATCHIIADKSSDPHHLNNIKNNVAYAESFLKENNIDFHTVAPEVSAGDFNQA
                     VLRYAKHKHAGLITLITQQNISLFEFLMQPAEQYFIANQEKIPVMCIHPDYNSIKYGS
                     VFAN"
     gene            2557..>3182
                     /locus_tag="PLO59_02160"
     CDS             2557..>3182
                     /locus_tag="PLO59_02160"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HQU99929.1"
                     /translation="MFRNQKEFIDKLENAGELVRIKTYVNPHLQIAEITDRVSKHYGP
                     ALLFENTGTPFPLLINAYGTEKRMAWALGVAHLDEIAQQIEALFKNITGPKHSFIDKL
                     KLLPQLGNIASWMPKEISGRGACQQVIMAQPDLTKLPVLTCWPMDGGPFVTLPVIQTR
                     DPQTGIRNVGLYRMQVFEPTLTGMHWHRHKVSARHFNVYKELGKRMPVV"
BASE COUNT         1145 a          631 c          555 g          851 t
ORIGIN      
        1 gatgctgcaa aaagaaatgc agaagaaagt ttgatcagag aacgtgcaca tgccgaagta
       61 atgcgcgtta cggcagtaat ggaaacccgc gaacaagaaa gaaaccggat agcaaaagaa
      121 ttacatgatg atttaggatc cggacttaca gaaattaaac ttctttgcga aatttataaa
      181 aagaacggta ttaacagcaa ttttgaaatt gaaaaaattg ccacatcgtc tggtgcatta
      241 attgacaaat tgagcgaaat agtatgggct acaaacccaa ggcacgatac attaagcagc
      301 ttattggttt acataagaca atatgctttt aattttttag ataatgccaa tattaaatgc
      361 catgcaaaca caatagacgt tccggctgtg cccttaacct ctgaattcag aagaaatgtt
      421 tttctaacaa ttaaagaagc gctaaataat gtggttaaat acgcttcaac agataaagtt
      481 gaaattaaaa tggaaattaa actgcccgaa aacggaaatc ccaatgcact tttgttaata
      541 cacataattg attttggcaa aggatttcaa aaccaaaaat taaacgaaac aacaaacggc
      601 ttatacaata tgaaagaaag aatggaaggt atttcaggta aatttgaagt tgacttaacc
      661 aaaggcgttc atataaaatt aacagcaccg cttttgcttt aactaatttt agcccgattc
      721 aaaaattgca tttagcgtct ataaatttaa attcgcagca ttaaaaccac ccaatcaaca
      781 aaacttatat gttacatatc gaaacggcca aaactatttt agtaccaacc gactttacac
      841 acgttggtga tagtgcttta aagcatgcca atatttttgc agcacgcacc ggcaaatcaa
      901 ttagcctgct gcatgttatt gcaaacgaaa ccgaaaaggc aaaagcactt gaaaagcttg
      961 atgttattgc cattggaaat accaaagcta ccaatatcga aaccatttcg atggtaagtg
     1021 taggtaccat ttttgaccaa ataggcgctg ttgccgatca ggtaaatgca gcttttgttg
     1081 ttatgggtac gcacggcata attggtatgc aaaaagtaac cggtagcaaa gcacttaaag
     1141 ttattactaa atcgcatgtg ccatttatag ttgtacaaaa gccacctgca cactcaacat
     1201 acctaaactt tgttattcct attagctatc aacgcgaaag caaacaaccc atatttaata
     1261 tagccgaact ggcacgaata tttggctcta cttgtcattt agtttatgca agtattaatg
     1321 acgagcagtt agaaatgaat attaaaaaca atgtagccta tgccgaaagt ttttttgatg
     1381 agcataatat caaatttgtc acgcataagt tagatgcaaa atttgcacag tttaacgagg
     1441 cctgcttaaa gtatgcaaaa caaatagagg ccgacctgat aacccttatt acagaacaaa
     1501 acattagcat ggccgaattt ttaatgcgcc ctgccgaaca gttttttatt gctaacgaac
     1561 tggccattcc ggttatgtgc attcatcccg actataaagc cgtgcaatac ggttccgtat
     1621 tcgcaaatta atacatacag caattatgaa taataacgat ttttcgaaaa ccattttagt
     1681 accctatgat tttactgtaa aagcagatgc tgccattaaa catgccaatt catttgctaa
     1741 aaaattaagt aaaacagtaa ccctcttaca tgtaattgcc gactatgacg aaaaaacaga
     1801 aacgcttgcg cgcttaaaag ccatagcagc caacaatcaa aaaataacag acataacaac
     1861 agaatactgt acaagtacag gcaccattta tgaccaaata gggcaggcag ccgatatttt
     1921 agatgcagca tttgtggtaa tggcaacaca tggcataaac ggagtgcaaa aagtgtttgg
     1981 cagtaaagca ttaaaggtaa ttaccaaatc acacaaacct tacatcgtag tacaagacga
     2041 accagccgac caattgtaca aacaaattat tgtacctatg agctatcagc ccgagtgtaa
     2101 acaacccctg tttcatatca tgaacattgc caagctgttt aatgcaacgt gccatataat
     2161 tgccgacaaa agcagcgacc cacatcattt aaacaacatt aaaaataatg ttgcttatgc
     2221 cgaatcgttt ttaaaagaaa acaatataga ttttcacacc gttgcacccg aagtatcggc
     2281 aggcgatttt aatcaggcag tacttagata tgccaaacac aaacatgccg gtttaattac
     2341 acttatcacc cagcaaaaca tttcgctatt cgaattttta atgcaacctg ccgaacaata
     2401 ttttattgcc aatcaggaaa aaataccggt tatgtgtatt catcccgatt ataattcgat
     2461 aaaatatggg tcggtttttg ccaattaaat gcaggtaata tctcgggcga aagcccgttt
     2521 ttttttattg ttatcaaata aattttaatc acaatcatgt ttcgtaatca aaaagaattt
     2581 attgataaac tcgaaaatgc aggcgaactg gttcgcataa aaacctatgt taacccacac
     2641 ctgcaaattg ccgaaataac cgatagagta tcgaaacact atggccctgc attattattc
     2701 gaaaacaccg gaacaccatt tccattatta attaatgcat acggtaccga aaaacgtatg
     2761 gcatgggcat taggtgttgc gcatttagat gaaatagcac aacaaataga agcactgttt
     2821 aaaaatatta ccggccccaa acattcattt attgataaat taaaactact tccacagttg
     2881 ggcaatatag ccagttggat gcctaaagaa attagtggcc gtggtgcatg ccaacaagta
     2941 attatggcgc aacccgattt aaccaaactg cctgtactta catgctggcc aatggatggc
     3001 ggcccatttg ttacactacc cgtaatacaa acacgcgacc cgcaaacagg catccgtaat
     3061 gtagggttat accgcatgca agttttcgaa cccacattaa ccggtatgca ctggcacagg
     3121 cataaagtta gtgcacgtca ttttaatgtg tacaaagaac tgggcaaacg catgcccgta
     3181 gt
//
LOCUS       DAOMAA010000105         3181 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_23456_length_3181_cov_2.723608, whole genome shotgun sequence.
ACCESSION   DAOMAA010000105 DAOMAA010000000
VERSION     DAOMAA010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23456_length_3181_cov_2.723608"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1195)
                     /locus_tag="PLO59_02165"
     CDS             complement(<1..1195)
                     /locus_tag="PLO59_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99930.1"
                     /translation="MGGTTIGISATGGDDNDNNSFVDNRIIRCRYGIGTRGAAATNLN
                     QNTIITDNLIGPTAFGADEIGQNGIFVQFENLCTISRNTVQFIGHDFANSISAFFDKA
                     GIAIGVNSWSNLTTTTASSSNISCTRNLVHDVCDERTNSAVGIIVGTTLSGPKTLNLI
                     ANNMIYNVRCNGTTGDQGAGIGHCGGPGDQIVFNSIYLTGDVDPTGTTTATTNPIIAG
                     ISKHVAAAADTAVFIKNNAIYVNISSNTTTLLKAPIIAPAAGYAYGSGGLNNNDYYIP
                     STANGHVIGVAGTGTTSFATLPLWQAVYTPAQDAASISVDPSFTSTTDLHINVSSPAV
                     NNLAVPLAAVINDFDNDTRSLTTPDIGADEYSPLVCSGTPTPGSIAPVSSSICTNGAG
                     VTLTLS"
     gene            complement(1345..1731)
                     /locus_tag="PLO59_02170"
     CDS             complement(1345..1731)
                     /locus_tag="PLO59_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99931.1"
                     /translation="MKKYTNHFYKPLCLFLIGMLCTVFAFGQNVNVTGALAGNGTYAT
                     LGAAFTAINVAGNNGSGPISVSIVANTTESATATLGAITWASVTVTATTPVTVTGNIT
                     GAIIRLNGADNVTIDGRIGGVGRIFL"
     gene            complement(1832..>3181)
                     /locus_tag="PLO59_02175"
     CDS             complement(1832..>3181)
                     /locus_tag="PLO59_02175"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99932.1"
                     /translation="SVTLQSGGQDMGLNMGTAGYYTFSFRDIGYANSEVYIGYTAAAP
                     VNVSFASQTFSGGQAVINITTSATPGTGEGVFVRYRNALNSFDAGTSLVEATGSGTTW
                     AANIPAQTCGSTTYYYIFTSTRTLAQLNSNNDTERSFALLRYDDNTGANYSYTCTAPI
                     VNCSGTNIDCLTPTGTASVSASGGSGSYTYLWSNAATTAAISGLAAGAYTVTVTDANA
                     CTATCSYTVTSNTTPPAALVTPASGVLTCTTTNINLTASGGNTYLWDDGSTNAVRNVT
                     AAATYTVTVTDAGNGCTATATAAITSDVTVPAVSASASPATICAGSTTTLTATGTGVS
                     YSWMPGSLSGSTVAATPASSTTYTVTATGSNGCTATATAAVTVNPNPILGTTMKSDAA
                     FVTTGTAGVTATPAGCTFLWAPDGETTNYIQNKAPGTYTVTVTGPGGCVKTRTIIIN"
BASE COUNT          867 a          663 c          700 g          951 t
ORIGIN      
        1 ccgataaagt aagtgtaaca cctgcaccgt ttgtacaaat acttgaagaa acgggagcta
       61 ttgagcctgg cgttggtgtt ccactgcaaa ctaaaggtga atactcatca gcacctatgt
      121 cgggtgtggt taagctgcgt gtgtcattat caaaatcatt aatgactgct gcaagcggaa
      181 ctgctaaatt attgactgcc ggtgaagaaa cattaatatg cagatctgtt gtggacgtaa
      241 agctcggatc caccgaaatg gatgcagcat cttgggctgg tgtatataca gcttgccaca
      301 atggtaaagt tgcaaaagat gtagtgcccg taccggcaac acctattaca tgaccatttg
      361 ctgtacttgg aatgtaataa tcgttattgt ttaaaccgcc tgaaccatat gcataaccag
      421 cagctggtgc aataattggc gcttttaaaa gtgtagttgt attcgaactg atatttacat
      481 aaatagcatt attcttaata aaaacggctg tatctgccgc tgcagcaacg tgttttgata
      541 tacctgctat aattggatta gttgtagctg ttgttgtgcc tgtggggtca acatcaccgg
      601 ttaggtaaat ggagttaaaa acaatctgat caccaggccc accgcaatgt ccaatgcctg
      661 ctccctggtc accagtagtt ccattacatc gcacattata aatcatatta ttggcaatta
      721 gattcaacgt tttagggcct gacaaggtgg tgcctactat aattcctacc gcagaattag
      781 ttctttcatc acatacatca tgtactaaat ttcgcgtaca agaaatgtta ctgcttgatg
      841 ctgttgttgt tgttaggtta ctccaagagt ttacaccaat tgcaatacca gctttatcaa
      901 aaaaggctga aatactattt gcgaaatcat gccctatgaa ttgtactgta tttctagaaa
      961 ttgtacataa attttcaaat tgtacgaaaa taccattttg accaatttca tcagcgccaa
     1021 aagctgttgg cccaattaag ttatcagtaa taatagtatt ttgatttaag ttagtagcag
     1081 cagcaccacg tgttccaatg ccgtaacgac atctgataat ccgattgtca acaaatgaat
     1141 tgttatcatt gtcatcgcca ccagttgcgg atattccaat tgttgttcca cccattaaaa
     1201 ttccaaaagt tgaatttgtt gtcgtatttt gtgtagcacc acaagcaatt tcaaggtttc
     1261 tgatggtgtt gttggtagct cctgcacctg cacctaaact tgatagccaa aaagctgctg
     1321 tagctgttgc ggtggaagag ttaattacag aaatatacgg cctacgccac caatacggcc
     1381 atcaatggtt acattatcgg caccatttag cctgataatt gcaccggtaa tattacctgt
     1441 aacggttacc ggtgtggttg ctgttacagt aaccgatgcc caggttattg cgcctaatgt
     1501 agctgtagca ctttcggtag tattagcgac tattgataca ctaattggcc ccgagccatt
     1561 gttgccggct acgttaatgg ccgtaaaagc agcacccaaa gtagcataag tgccattacc
     1621 tgccagtgcg cctgttacat ttacattttg cccaaaagca aaaaccgtac aaagcatacc
     1681 tattaaaaaa aggcaaagtg gtttgtaaaa atgattagta tattttttca tcgtatgagt
     1741 gtttttaatt gcagtgcaac tatttgtgtg ggtgtttgat gtttgagcca acattaattt
     1801 atatgggtat ttcatattta gtcctccttt tttaattaat gattatggtt cgtgttttaa
     1861 cacagccacc cggcccggtt actgttacag tataggtacc cggtgctttg ttttgtatgt
     1921 aattggtggt ttcgccatcg ggtgcccata aaaatgtaca tcctgcaggt gtagcagtta
     1981 caccggctgt acctgtagta acaaaggctg catcgctttt catggtagta cctaaaatag
     2041 gattggggtt aaccgtaaca gcagcagtag ctgtagcggt gcatccatta ctgcctgtgg
     2101 ccgtaacggt gtaagttgta ctacttgcag gcgttgcggc aaccgtactg ccacttagcg
     2161 aaccgggcat ccaactataa ctaacacccg tacctgttgc cgtaagtgtt gttgtgctgc
     2221 ctgcacaaat ggttgcaggg cttgccgatg cacttactgc tggtactgta acatcgctgg
     2281 taatagcagc tgtggcagtt gctgtacatc cattgccggc atctgttact gttacggtat
     2341 aggttgctgc tgctgtaaca ttacgcacag cattggtgct gccatcgtcc cataaataag
     2401 tattgccgcc tgaagcagtt aaattaatat tagttgtagt acaggtaagt acaccgcttg
     2461 caggtgtaac caaagctgcc ggaggtgtgg tattgcttgt aacggtatag gaacaagttg
     2521 ctgtgcatgc attggcatcg gttacggtta cagtatatgc gcctgcggct aagccactaa
     2581 ttgcagctgt ggtagctgca tttgaccata aataggtata tgaacccgag ccaccacttg
     2641 ccgaaaccga agctgtaccc gttggcgtta agcaatcaat attggtaccg ctgcaattaa
     2701 caattggtgc tgtgcaggta tagctgtaat tagcacctgt attatcatca taacgcagta
     2761 atgcaaagct gcgttcggta tcgttgttac tatttaactg tgccagtgtg cgtgtactgg
     2821 taaaaatgta ataatatgtt gttgaaccac aagtttgtgc aggtatgtta gctgcccagg
     2881 tagtgcccga gcctgttgct tcaaccaatg aagtaccggc atcaaatgaa tttagggcat
     2941 tacggtaccg aacaaataca ccttcgcctg tgcctggtgt ggcactggtg gttatattta
     3001 ttactgcctg ccctccactg aatgtttgag aggcaaagct tacgttaaca ggtgctgcgg
     3061 ctgtatatcc aatataaact tcgctgttgg catagccgat atcacgaaac gagaaagtat
     3121 agtaaccggc agtgcccata tttaagccca tgtcctgacc accactttgt agtgtaacac
     3181 t
//
LOCUS       DAOMAA010000106         3174 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_23557_length_3174_cov_2.737416, whole genome shotgun sequence.
ACCESSION   DAOMAA010000106 DAOMAA010000000
VERSION     DAOMAA010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23557_length_3174_cov_2.737416"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="PLO59_02180"
     CDS             <1..747
                     /locus_tag="PLO59_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HQU99933.1"
                     /translation="ATIASIAAPMFKKMSLELGGKNPNIIFADCDFEHALATTVKSSF
                     SNQGEICLCGSRIFVERPIYEKFKQGFVAATKALKVGDPQQDNFMGAIVSEMHYHKIL
                     SYIQLATEEGGTILTGGRAIKPEGRCANGYFIEPTIIEGLSYNCRTNMEEIFGPVVTI
                     MPFDTEAEVLQYANATKYGLAATVWTTNLNKAHRVAALLHSGIIWINCWLLRDLRTLF
                     GGMKNSGLGREGGFEALHFFTEEKNVCIKF"
     gene            complement(854..1279)
                     /locus_tag="PLO59_02185"
     CDS             complement(854..1279)
                     /locus_tag="PLO59_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HQU99934.1"
                     /translation="MRSVTKEYSNGQVTIVWQNSLCAHSAICFKGLPQVFNPNVRPWI
                     NATGATTQQIIDQVKRCPSGALSIKELADQSIQSTDNQTTLCEVTHNGPLLIHGSIKV
                     VDAMGNETIKEKTTAFCRCGASHKKPYCDGSHRKIGFEG"
     gene            complement(1286..1825)
                     /locus_tag="PLO59_02190"
     CDS             complement(1286..1825)
                     /locus_tag="PLO59_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018278214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HQU99935.1"
                     /translation="MATIKWGIDPAHSEIGFKVRHLMISNVKGFFSNYNEVIETSDDE
                     DFTQAKIELTLDATSVSTGNDDRDKHILSADFLDVANYPEIKFVGTRSENVDNDGSYE
                     LFGNLTIRSVTKEVKLDIEFGGVMKDPWGNHKAGINVNAKFNRADFGLVWNAALETGG
                     VLVSDEVRIACEIQLVRQS"
     gene            complement(1896..2576)
                     /locus_tag="PLO59_02195"
     CDS             complement(1896..2576)
                     /locus_tag="PLO59_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017256724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HQU99936.1"
                     /translation="MVQPYVLQTAESRDYAQLSWLSSFHSFSFTSYHDDNKIGLGLLC
                     VLNDVVAPGKGFGAHPHQYMEIILMPASGALLHKDNTGRHEIINTSDVQIMSAGSGIN
                     HSETNASNRDEVRFFQIWIYPKTENTKPAYAQQSFTPQQKHNVLLPVVSPNGVDGSLP
                     INQDAWIFLSHADAGISLNHSTKQNGNGLYVFIIEGEAMVNDVILKRRDAIGLPDGRA
                     QITTSTKA"
     gene            complement(2631..3068)
                     /locus_tag="PLO59_02200"
     CDS             complement(2631..3068)
                     /locus_tag="PLO59_02200"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HQU99937.1"
                     /translation="MADTKINIAIDDVIALAQCTFYRSLTRSFKDSGKNITVEQWTML
                     KHIYENKGITQQAIANCSNKDKHSVCRLIDNMQKQQWVVRMPDKADKRINHLYLTEQG
                     KKLHKQLYDLVQNYKSKAVKGISVTELKACSQILNRVTANMAR"
BASE COUNT          904 a          628 c          580 g         1062 t
ORIGIN      
        1 gcaaccattg caagcattgc agcacctatg tttaaaaaaa tgtcgttaga gttgggcggc
       61 aaaaacccca acatcatttt tgccgattgc gattttgaac atgctttagc aaccaccgta
      121 aaatcatcgt tcagcaatca gggcgaaata tgtttatgtg gctcacgtat ttttgttgaa
      181 cgacctatct atgaaaaatt taagcaaggc tttgttgctg caactaaagc gttgaaagtg
      241 ggcgatccgc agcaagataa ttttatgggt gctattgtat cagaaatgca ttaccataaa
      301 attttatcat acatacaatt agcaaccgaa gaaggcggca ccatattaac cggagggcgt
      361 gctataaaac ctgaagggcg ttgtgccaat ggatatttta ttgagccaac tattatcgaa
      421 ggtttatcat ataactgccg taccaatatg gaagaaattt ttggccccgt tgtaaccatt
      481 atgccttttg ataccgaggc cgaagtttta caatatgcca atgctactaa atatggattg
      541 gctgcaaccg tatggacaac taatttaaat aaagctcatc gtgtggccgc attattacac
      601 agtggtatta tttggataaa ctgctggtta ttgcgcgact tacgtacctt atttggaggc
      661 atgaaaaatt cgggcctggg gcgtgagggt ggctttgaag cattgcactt ttttacagaa
      721 gaaaaaaacg tttgtattaa gttttaattt aaacgttggt gtgtagtcag cccaaacgcg
      781 ggtttagtgg atttataaaa acaaaagagg cgcagtaaaa aaattactgc gcctcttttg
      841 tttttataaa tcattatcct tcaaagccta ttttacgatg actaccatcg caatacggtt
      901 ttttatgaga tgccccacaa cggcaaaaag cggttgtttt ctctttaatg gtttcattgc
      961 ccattgcatc aaccactttt atggagccat gtatcaacaa aggtccatta tgtgtaacct
     1021 cgcataaagt ggtttgatta tcggttgatt gtatggactg atcagctaat tctttaattg
     1081 ataatgcacc tgacggacat cgttttacct gatcaataat ttgttgcgtt gttgccccgg
     1141 ttgcattaat ccatgggcgt acgtttgggt taaatacttg tggcaatcct ttaaaacaaa
     1201 ttgctgaatg ggcacacaaa ctattttgcc atacgatggt aacttggccg ttactatact
     1261 cttttgttac agacctcata gcaaactaac tttgacgtac caattgtatt tcgcaggcaa
     1321 tgcgtacttc atcactaacc aaaacacctc cggtttctaa agcggcattc cacaccagac
     1381 caaaatcagc acgattaaat ttggcattta catttatgcc ggccttatgg ttaccccatg
     1441 ggtctttcat tacaccacca aactcaatat ctaactttac ttccttagtt accgacctta
     1501 tggtaaggtt accaaacaat tcatagctgc catcattatc aacattttcg ctacgggtac
     1561 caacaaattt tatctcgggg tagtttgcaa cgtctaaaaa atcggcactt aaaatatgtt
     1621 tatccctatc atcattgccg gtgctaaccg aggttgcatc caatgttagt tctattttag
     1681 cttgtgtaaa atcctcgtca tcactcgttt cgattacctc attataattc gaaaaaaatc
     1741 cttttacatt actaatcatc aagtggcgta ctttaaagcc tatctcactg tgagcggggt
     1801 caatacccca tttaattgtt gccataaatt atctgattta aagtttatag tgtgtaattg
     1861 taacattatt tcatcggtac atcaagcaat aatatttatg cttttgttga ggtggtaatt
     1921 tgggcccggc catctggcaa acctatggca tcgcgtcttt ttaaaattac gtcattaacc
     1981 attgcttcgc cttcaataat aaatacatac aagccgttgc cattttgttt tgtgctgtga
     2041 tttaaactta tgccggcatc agcatggctt aaaaatatcc aggcatcctg gttaattggc
     2101 aaactaccat ctacaccatt tggcgatact acaggtaata atacattatg tttttgttgc
     2161 ggggtaaaac tttgttgcgc ataggcgggt ttggtgtttt ctgttttggg ataaatccat
     2221 atttgaaaaa accgaacctc gtccctattt gaagcatttg tttcggagtg gttaataccg
     2281 ctacccgcac tcattatttg tacatcactt gtattaataa tttcatgacg acccgtgttg
     2341 tctttatgta ataaagctcc gctggctggc attaaaatta tttccatata ttgatgtgga
     2401 tgtgcgccaa aacctttgcc cggtgccact acatcattta atacgcataa aagtcctaac
     2461 ccaatcttgt tatcatcatg ataggatgtg aaactgaatg aatgaaaact acttaaccag
     2521 cttagttgtg cataatctct gctttcggct gtttgtaata cgtatggttg caccatgcga
     2581 ttattactgt taacacatta gttgcatata caactattaa gcctctcaca ttaccgagcc
     2641 atattagcag taactctgtt taaaatttgg ctgcaagctt ttaattcggt taccgaaatg
     2701 cctttaaccg ccttactttt ataattttga accaaatcat atagttgttt atgcaacttt
     2761 ttaccttgtt cagtaaggta taaatggttg atgcgtttat ctgctttgtc gggcatccgt
     2821 actacccatt gttgtttttg catattatcg ataagcctgc aaacactgtg tttatcttta
     2881 ttgctacagt ttgcaatggc ctgttgcgta atgcctttat tttcataaat atgtttgagc
     2941 atagtccact gctcaaccgt aatatttttt cctgagtctt taaagctacg ggttaatgag
     3001 cggtaaaaag tacattgtgc taaggcaata acatcgtcaa tggcaatatt aattttagta
     3061 tcggccatgt tatgaaacaa ctattggtgt agttaatgta atgcctgttt ctttaatttt
     3121 tgcatatcca ccatcaacat ttacaatgtt gtaaaatcct ttggcatgca tcat
//
LOCUS       DAOMAA010000107         3160 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_23730_length_3160_cov_3.178100, whole genome shotgun sequence.
ACCESSION   DAOMAA010000107 DAOMAA010000000
VERSION     DAOMAA010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23730_length_3160_cov_3.178100"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="PLO59_02205"
     CDS             complement(<1..711)
                     /locus_tag="PLO59_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015025732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HQU99938.1"
                     /translation="MSDKSQMIQNDVSFETFKQQVLDDYKLCHISRQASLTGRKEVLT
                     GKAKFGIFGDGKELAQIALAKAFQNGDFRAGYYRDQTFMFAIGALTVQQYFAQLYANT
                     QDDADPSSHGRLMNGHFSTKLLDHDGQWKNLMAQKNSSADISPTAGQMPRLLGLAYAS
                     KFYRHNKDLQTEAFQKFSATGNEVAFGTIGDASTSEGLFWETINAAGVLQVPMAISVW
                     DDGYGISVSQKYQTTKQSI"
     gene            complement(855..>1490)
                     /locus_tag="PLO59_02210"
     CDS             complement(855..>1490)
                     /locus_tag="PLO59_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99939.1"
                     /translation="GLTYMQKFLSKVEPSKIIPLDYRYHGRMLSRTGSDSLGIEQLKL
                     AIQYDSTDADMWQDMGEALNRLKKYCEAAGAFKQKLSISKNPSALDYFYLTKAYYYCK
                     DYANADSAAQVIINLKASSHIGWLWRGNANAKMYTDDLEKPLPFYQRVAEISDSTGAK
                     IPNSDAITAYRFLGVYNIKKENNVLAKDYYNKILARDPKDADAIETMKRLK"
     assembly_gap    1491..1500
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1501..2436)
                     /locus_tag="PLO59_02215"
     CDS             complement(<1501..2436)
                     /locus_tag="PLO59_02215"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQU99940.1"
                     /translation="MKKLSSLSVATLFATGVFAQTVTDGLRALDNDQLIKAKNTFQSL
                     LGSKPEEATIAYYQLASLYMDQKTTDSAELVVNRMQTAYPTNAMSKVAAGKLNLYKKN
                     ATAAKISFDEALKISANKDAKVMAWIADAYLTTENTDGAAALQVLANATKLDPKNAEL
                     YLLTGDAHKLDLNSGGPAITNYEKAIELNAQLVKANQRIGLVYSQARNYQSSSEYFEK
                     SLGMDANYAPTLRDYAMLNYNFKQYEKSRALYEKYLAIADTTELTLTRYVYILFMCKD
                     YPAATAMINTLMVRDANNNLLNRLLAYSYAEQKQDS"
     gene            complement(2594..>3160)
                     /locus_tag="PLO59_02220"
     CDS             complement(2594..>3160)
                     /locus_tag="PLO59_02220"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HQU99941.1"
                     /translation="SILTMQHVYDILSGKIKKWDEINTQNKSGNIQIIFDHKNSSTAR
                     YVKDSILKGSTFAENVFAVDSNVAVINYVSTHPNAIGVLGVSWISDGDDPTTLGFLKM
                     VRTLAIVAPDSTAGAGKSYQPYQAFIATNYYPYTRAVYIISREARSGLGTGFAAFVAG
                     DKGQRIILKSGIMPATQPVRIVGFRNNN"
BASE COUNT          845 a          596 c          600 g         1109 t
ORIGIN      
        1 aatgctttgt ttggtggttt gatatttttg cgatactgaa atgccgtaac catcatccca
       61 aacacttatt gccatgggta cctgaagcac tccggccgca tttatagttt cccaaaacaa
      121 gccttcgctg gtgcttgcat cgcctatggt gccaaaggca acttcgttac cagtggccga
      181 aaatttttga aatgcctctg tttgcaaatc cttattatgg cgataaaatt ttgatgcata
      241 agctaaacct aacaggcgtg gcatttgtcc ggcagtgggc gatatatcgg ccgatgaatt
      301 tttttgtgcc attaagtttt tccattggcc atcatggtca agtagtttgg ttgagaaatg
      361 cccattcatt aaacggccat gagatgatgg gtcggcatca tcttgcgtgt tggcatatag
      421 ttgtgcaaaa tattgttgta ctgtaagtgc accaatggca aacataaaag tttggtcgcg
      481 gtaatagccc gctctgaagt cgccattttg aaatgctttg gctaaggcta tttgtgccaa
      541 ttcttttcca tcgccaaaaa tgccaaactt ggctttgcct gtaagcactt ctttacgacc
      601 tgttaagctt gcctgtcggc tgatatggca tagtttataa tcatctaata cctgttgctt
      661 aaacgtttca aaacttacat cgttttgaat catttgactt ttatcactca tacaaaatta
      721 attttaatgc taaacttcaa tatccaattg tttattggaa ctgggtaaaa atataaaatt
      781 aagcaagttg attttatgca tttcaatttg catacaaaaa taaaaactcc gaagtgttgc
      841 ttcggagttt ttaactattt aagtcgtttc attgtttcta ttgcatctgc atctttgggg
      901 tcgcgtgcta aaattttatt gtagtaatct ttagcaagta cattgttttc ttttttaata
      961 ttataaacac ctaaaaatct ataggctgtt atggcatcgc tgttcggtat ttttgcgcct
     1021 gtcgaatccg atatttcagc aacgcgttga tagaatggta atggtttttc taaatcatct
     1081 gtgtacattt ttgcattggc atttccacgc cataaccatc ctatgtgact cgaagctttt
     1141 aagttgatga ttacctgtgc tgcactgtca gcgtttgcat aatctttaca gtagtaataa
     1201 gctttggtta aataaaaata gtcgagtgct gatggatttt tcgagatgct gagtttttgt
     1261 ttaaatgcac cagctgcttc gcaatatttt tttaagcggt taagtgcctc gcccatatct
     1321 tgccacatat cggcatcggt cgaatcatat tgtattgcta attttaattg ttctatacct
     1381 aacgagtcgc tgcctgtgcg tgatagcata cggccatgat atctataatc taacggaatt
     1441 atttttgagg gctcaacctt tgataaaaac ttttgcatgt atgtaagtcc nnnnnnnnnn
     1501 actgtcttgc ttttgttctg cataggaata tgctaacaaa cgattgagta aattattatt
     1561 tgcatcacgt accattaatg tatttatcat ggctgtagca gcaggatagt ctttacacat
     1621 aaacaagata tatacataac gtgttagcgt aagttcggta gtatcggcaa tggctaaata
     1681 tttttcatac aaggctcttg atttttcgta ttgtttaaag ttatagttta gcatagcata
     1741 gtcgcgcaat gtaggcgcat agtttgcatc catacccagc gatttttcga aatactcact
     1801 tgagctttga taatttcgtg cttgtgagta caccaagcct atacgttgat tggcttttac
     1861 caattgcgca tttaactcta tagctttttc ataattggta attgccgggc cgccactgtt
     1921 taaatcaagt ttatgtgcat cgccagttaa taggtagagt tctgcattct taggatctaa
     1981 cttggtagcg tttgctaata cttgcaatgc tgctgctcca tcagtgtttt cagtggtcag
     2041 ataggcatct gcaatccagg ccataacttt agcatcttta ttagctgaaa tttttagggc
     2101 ttcgtcaaaa ctaattttgg ctgcagttgc attcttttta tacagattta atttacctgc
     2161 tgctacttta ctcattgcat tggttgggta ggctgtttgc atccggttaa ctacaagttc
     2221 ggccgagtcg gtagtttttt gatccatata caaactggca agttggtaat aagcaatagt
     2281 tgcctcttcg ggcttactgc ccaaaaggct ttgaaaagta ttttttgctt tgattagttg
     2341 atcgttatcc aatgcacgta agccatcagt aactgtttgt gcaaacacac ctgttgcaaa
     2401 aagtgtagct acacttaagg agcttaattt tttcatattt aaaaattctt agtaatattt
     2461 gatttaatgc aagaatgtaa ccaaacaagc atcgttctta tttatgaggc gcaagaataa
     2521 aataatttag ctaaatttca ttttgcctct actgtcattg tttcgttatg gtttaatata
     2581 acgctgccag gctttagttg ttgtttctaa aaccaacaat tcgtaccggt tgtgttgcag
     2641 gcattatacc tgatttaaga ataatacgtt gccctttatc gcctgcaaca aatgcagcaa
     2701 aaccggtgcc taatccacta cgcgcttcgc gacttattat ataaacggct cgtgtatatg
     2761 gataatagtt tgtagcaata aaagcttggt aaggctggta acttttacct gcaccggctg
     2821 tgctatcggg tgcaacaatt gccagtgtac gcaccatttt taaaaaaccc aaagtagttg
     2881 ggtcatcgcc atcgcttatc cagctaacac ctaacacgcc aattgcattg gggtgtgtgc
     2941 ttacgtaatt aattacggcc acattactat caactgcaaa aacattttcg gcaaatgtgc
     3001 ttccttttag tatgctatct tttacgtagc gggcagtact cgagtttttg tggtcgaaaa
     3061 ttatctgaat attacctgat ttgttttggg tgtttatctc gtcccacttt tttatttttc
     3121 cactcaaaat atcataaaca tgttgcatgg ttagtatact
//
LOCUS       DAOMAA010000108         3151 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_23867_length_3151_cov_2.917636, whole genome shotgun sequence.
ACCESSION   DAOMAA010000108 DAOMAA010000000
VERSION     DAOMAA010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23867_length_3151_cov_2.917636"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="PLO59_02225"
     CDS             complement(<1..455)
                     /locus_tag="PLO59_02225"
                     /inference="COORDINATES: protein motif:HMM:NF024912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HQU99942.1"
                     /translation="MCGIVAAIAFTEAGKNKLGLIDKATEKLAKRGPDAQGIYHHNHI
                     AIGHRRLSIIDTSQQASQPMFDNSSRYVIAFNGEFFNYQQYRKQLISQGFTLNNLSDT
                     EVLLQLYIQEGEKFLEKINGFFALIIYDTVTQTVFIARDRMGVKPLYLY"
     gene            complement(462..797)
                     /locus_tag="PLO59_02230"
     CDS             complement(462..797)
                     /locus_tag="PLO59_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018478332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HQU99943.1"
                     /translation="MSNKNKLLNGVVYSTNKSYQYETNEANPVETLPPNKQILKIRLE
                     TKHRGGKEATVISNFVGLDNDAEKLAKILKTKCGVGGSYKDGEIIIQGDMRNRIKLLL
                     IEMGYQTKG"
     gene            complement(918..1388)
                     /locus_tag="PLO59_02235"
     CDS             complement(918..1388)
                     /locus_tag="PLO59_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99944.1"
                     /translation="MKNLLFTFFLFTATIIANAQIDLNKLGGDAIKGLDMGNLNMGSV
                     IGELGKGIKPTMLSDAWQNKVGDWTKNVANIKDVPGAVKSLSGLVKNIKPEAFTSGFA
                     ALKGDWLKNLSRIKDMTGLGNSLKTLMGGLKPEALTSDFLKKQVNLGNALNLLK"
     gene            1479..>3151
                     /locus_tag="PLO59_02240"
     CDS             1479..>3151
                     /locus_tag="PLO59_02240"
                     /inference="COORDINATES: protein motif:HMM:NF025068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HQU99945.1"
                     /translation="MKKIKLLFQQSVLIVCLLGVTLAHAQNRNRLSVMVQNETFKTVF
                     TPVNLFKLTTVANAEQFVNNGTGLTVDATQLGNLFASKPTAVSLEIPTANGRTFTLDV
                     VKQNILSNDFKVSTQNQNQVSYTPGVYYRGIIKGDFNSIAAVSIFNDDVMIMFSNAEG
                     NFNLGKVNNQHNQYFLYNDHLVVNKPAIDCSTTDGDSKSDISIEKNGGNTTSSVVCKT
                     VKCYYEADFAMYNAFSSNTTTTSNYVTSIFNVKSTLYANDGMNIEISEIKVWTATDPY
                     ASFTSTSTTNNAFITQTGSTFNGDMANLLTTRNLGGGIAQGFSGLCNKQQAHCTSMIY
                     TTYSPFPTYSWTVMVITHEMGHLMGSRHTHACVWNGNNTAIDGCSGFVEGSCTLPGIP
                     SGGGTIMSYCHQQSVGINLNLGFGPQPGGLLLNNVNTATCLTGSGASTPTGLNTTAIT
                     SSSATLNWNAVSGATQYTVEYKLATASTYTLLGTVTSNSAALSGLTSSTAYDWRVSAD
                     CSPFSAPATFTTSGLAGCGVPVGLGADNISSSTARIFWSDYVGAVNYKTR"
BASE COUNT          944 a          587 c          571 g         1049 t
ORIGIN      
        1 tcatacaaat acaaaggctt aacgcccata cggtcgcgtg caataaagac tgtttgtgtt
       61 acggtatcat atataattaa agcaaaaaag ccgtttattt tttctaaaaa tttttcgcct
      121 tcttgtatgt aaagctgaag caatacttcc gtatcgctta ggttgtttaa tgtaaagcct
      181 tgcgatatca gttgttttcg atattgctga tagttaaaaa actcgccatt aaatgcaatt
      241 acatacctgc tactattatc aaacatgggc tgcgatgctt gttggcttgt atcaattatt
      301 gataatcgcc tgtggccaat tgcaatgtgg ttatgatggt aaatgccttg cgcatcgggt
      361 ccacgtttag ctaacttttc tgtagcttta tcaattaaac caagtttgtt tttaccggct
      421 tcggtaaaag ctatggctgc aactattcca cacatactat attatccttt tgtttgataa
      481 cccatttcaa taagcaataa ttttatgcga ttacgcatat cgccttgtat aataatttcg
      541 ccatctttat aactgccgcc cacaccacat tttgttttta gaattttagc aagtttttcg
      601 gcatcattgt caagtcccac aaagtttgaa attacagtag cctctttacc gccacggtgt
      661 ttggtttcga gcctgatttt taaaatttgt ttgttgggag gtaatgtttc aacggggtta
      721 gcttcattgg tttcatattg ataggattta ttggttgaat atacaacacc gttaagcaat
      781 ttgtttttgt ttgacatggc aactttattt ttttgaaagg tgctttataa tttcaaaata
      841 cattttacaa acatctatat taactgtaaa taaaaaagcc ccctatgtgg aggctttttt
      901 attttattac agtttgttta tttaagcaag tttaaagcat tacccaaatt aacttgtttt
      961 ttaagaaagt cgctggttag cgcctcaggt ttaagtccac ccattaatgt ttttaacgag
     1021 ttgcctaagc ctgtcatatc cttaatacgc gaaagatttt taagccaatc gccttttaat
     1081 gccgcaaaac ccgatgtaaa agcctcgggc tttatattct taaccaaacc gctaagcgat
     1141 tttacagcac caggcacatc tttaatatta gctacatttt ttgtccagtc gccaactttg
     1201 ttttgccatg catccgatag catagtgggt ttaattcctt tgcctaattc gccaataaca
     1261 ctgcccatgt ttaagttgcc catatctaaa cctttaatgg catcgccacc taatttgttt
     1321 aagtcaattt gggcattagc aataatagta gcagtaaata aaaagaatgt aaaaagtaag
     1381 tttttcataa cgttgttttt agatgattaa atattgaaaa aaattaaatg gagatacttt
     1441 tttaaattat agattcgtgc cataaacgaa acaaacttat gaaaaaaatc aaacttctat
     1501 ttcaacaaag tgttttaatt gtttgcctct taggcgtaac attagcacat gcgcaaaacc
     1561 gtaatcgctt atcggttatg gttcaaaacg aaacctttaa aacggttttt actcccgtta
     1621 atttgttcaa actaacaaca gttgccaatg ccgagcaatt tgtaaataat ggcacaggtt
     1681 taactgtgga tgctacacaa ctaggtaatc tgtttgcctc aaaacctact gctgttagtt
     1741 tagaaattcc aactgctaat ggccgcacgt ttactttgga tgtggttaag caaaacattt
     1801 tgagtaacga ttttaaggtt tctacacaaa accaaaatca ggtaagctac acaccaggtg
     1861 tttactatcg aggtataatt aaaggcgact ttaattcaat tgcagcagta agtattttta
     1921 atgatgatgt tatgattatg tttagcaatg ccgaaggcaa ttttaattta ggtaaagtaa
     1981 acaatcaaca caatcagtat tttttatata acgatcacct tgttgttaac aagcctgcca
     2041 ttgactgtag tactacagac ggtgattcga aatcagacat ttctattgag aagaatggtg
     2101 gcaatactac cagcagtgta gtttgtaaaa cggttaaatg ctattatgaa gctgattttg
     2161 caatgtacaa tgcatttagc tcgaacacaa ccaccacttc gaattacgtt accagcatat
     2221 ttaatgttaa aagtacattg tatgctaatg atggtatgaa catagaaatt tcggaaatta
     2281 aagtttggac tgcaaccgac ccttatgcat cattcaccag tacaagtact actaacaatg
     2341 catttataac ccaaaccgga agtactttta atggcgatat ggccaacttg ttaactacac
     2401 gtaatttagg tggtggtatt gcacaaggct ttagcggttt atgcaataag caacaagcac
     2461 actgtaccag tatgatttat actacgtatt cgccatttcc aacttattcg tggacagtaa
     2521 tggtaattac acatgaaatg ggtcatttaa tgggttcgcg tcatacacat gcttgcgttt
     2581 ggaatggtaa caatacagcc attgatggtt gctcgggctt tgttgaaggc agttgtacat
     2641 tacccggaat tccttcaggt ggtggcacca tcatgagtta ttgtcatcaa caatcggtgg
     2701 gtattaattt aaatttgggt tttggtccgc agccgggcgg tttactttta aacaatgtta
     2761 atacagcaac ttgtttaaca ggcagcggtg cttcaacacc aacaggttta aatacaacgg
     2821 ccattacatc ttcatcggct acattaaatt ggaatgccgt tagtggtgca acccaatata
     2881 cggtagaata taaattggca acagcatcaa cttacaccct tttaggaact gtaacaagca
     2941 acagcgctgc gctttctggt ttaacaagca gcacagctta cgattggcgt gtatcggccg
     3001 attgttcgcc attttcagca ccggctacct ttacaacttc aggcttagca ggttgtggtg
     3061 taccggttgg tttaggtgcc gataatattt catcttctac agcgcgcatt ttctggtcgg
     3121 attatgttgg tgctgttaat tataaaacac g
//
LOCUS       DAOMAA010000109         3140 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_24020_length_3140_cov_3.022690, whole genome shotgun sequence.
ACCESSION   DAOMAA010000109 DAOMAA010000000
VERSION     DAOMAA010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24020_length_3140_cov_3.022690"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="PLO59_02245"
     CDS             <1..190
                     /locus_tag="PLO59_02245"
                     /inference="COORDINATES: protein motif:HMM:NF014232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HQU99946.1"
                     /translation="DAGSSPEQITSKPVETKKPVINMNEFSGLSASQLQEMLAKALDD
                     EAYERASRIRDELKKRKK"
     gene            207..1001
                     /locus_tag="PLO59_02250"
     CDS             207..1001
                     /locus_tag="PLO59_02250"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011584676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HQU99947.1"
                     /translation="MKQYHDLMQHVLLNGINKTDRTGTGTTSVFGYQMRFDLSAGFPL
                     VTTKKLHTKSIIHELLWFISGSTNINYLTQNGVKIWDEWADENGNLGPVYGKQWRSWQ
                     SPDGVVIDQLSTLIEMIKKSPDSRRLIISAWNVSDIGKMALPPCHTMFQFYVANNKLS
                     CQLYQRSADIFLGVPFNIASYALLTMMIAQVCNLQPGEFVHTLGDAHLYTNHIEQAQL
                     QLTREIRPLPQMLLNKSITSIFDFKFDDFALQDYNPHPHIKAEVAV"
     gene            complement(998..1789)
                     /locus_tag="PLO59_02255"
     CDS             complement(998..1789)
                     /locus_tag="PLO59_02255"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HQU99948.1"
                     /translation="MAIPAFRNYYFGLYKKVIKPYSLFKNVVVKTKHQGIIFQLHLND
                     WIAQNIYFLRSYEKPELAYFKSQLKPNSVFIDIGANIGLYALNAAKVCLQGKVYAFEP
                     FKINSKALLNNAHLNGLNIIWFDKAVSNHNNSLNLFYSESQQNLGAVSILNTGGQQFT
                     VQATTLDAFVSEQQIDKIDFIKLDIEGSELAALEGMRYVLQQYNLQLMVEIDLEILAG
                     LAHNAADIHNYMATLKYKAYYLQADGLLTEHANLAVSKNVIFKRS"
     gene            1964..2449
                     /locus_tag="PLO59_02260"
     CDS             1964..2449
                     /locus_tag="PLO59_02260"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012781579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HQU99949.1"
                     /translation="MKVTLVVAVSSNWVIGQNNKLLWHLPNDLRHFKNITTGGTIIMG
                     RKTFESIGKPLPNRVTIVISTQKDFDAKGAIVVNSLELALEKCKHKDEVFIVGGGEIY
                     KVALPIAQQIYLTQVQQSFDGDTYFPEIDFSQWVVTKSESFDVDDKHPFAYKFITLIR
                     K"
     gene            complement(2450..>3140)
                     /locus_tag="PLO59_02265"
     CDS             complement(2450..>3140)
                     /locus_tag="PLO59_02265"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HQU99950.1"
                     /translation="QLPGMQEALLDKLFELKKPVVVLLVGGSAVTMSWLQKADAVMQV
                     WYPGQQGGHAIASVLYGTSPSGKLPFTWPVSEAQLPLVYNHKPTGRGNDYTDLSGKPL
                     FPFGYGLSYSTFSYSNLQLSKAIIKSDENLTVSCSITNTGIVKADEVIQLYIKDKVAS
                     YAQPVMALKGFKRLQLQPNESKKVTFKILPHMLTVLDDNLKPVVEPGFFEVLIGASSA
                     DIKLRTEFEVK"
BASE COUNT         1013 a          575 c          557 g          995 t
ORIGIN      
        1 cgatgccggt tcaagccctg agcagataac atcaaaacct gttgaaacaa aaaagcccgt
       61 tataaacatg aatgagttta gcggattatc ggcatcgcaa cttcaggaaa tgcttgccaa
      121 ggctttggat gacgaggcat acgaacgcgc atccagaatt cgtgatgaat taaagaaacg
      181 taaaaagtag atatacaact acctgcatga aacaatatca tgatttaatg caacatgtgt
      241 tgctaaatgg cataaacaaa acggatagaa ccggaaccgg caccacttct gtttttggct
      301 atcaaatgcg attcgatttg agtgctggtt ttccgttagt tactaccaaa aaattgcata
      361 ccaaaagtat tattcatgag ttgctttggt ttattagtgg tagcaccaac ataaattact
      421 taacacaaaa tggcgttaaa atatgggatg aatgggctga tgaaaatggc aatttaggcc
      481 ctgtgtacgg caaacaatgg cgtagttggc aaagtcctga tggtgttgtt attgaccaat
      541 taagtacttt aattgaaatg attaaaaaat cgcccgactc gcgcaggctc attatcagtg
      601 cttggaatgt aagcgatatt ggcaaaatgg ctttaccacc gtgccacacc atgtttcagt
      661 tttacgttgc caacaacaaa ttaagttgtc aattatatca acgcagtgcc gatatatttt
      721 tgggggttcc atttaatatt gcatcttatg ccttgttaac tatgatgatt gcacaagttt
      781 gtaatttaca accgggtgaa tttgtacata ctttaggcga tgcacattta tataccaacc
      841 atattgagca agcacagcta caacttacac gcgaaataag acccttgcct caaatgctat
      901 taaacaaatc tataacttcg atattcgatt ttaaatttga tgactttgct ttacaggatt
      961 ataatccgca tccacacata aaggccgaag tagctgttta acttctttta aaaattacat
     1021 ttttacttac ggctaagttt gcatgctcag ttagtaaacc atcagcttgt aaatagtaag
     1081 ctttgtattt taaagtagcc atgtagttgt gtatatctgc tgcattatgg gccaaaccag
     1141 ctaatatttc taaatcaatt tcaaccataa gctgcaaatt gtattgttgt aaaacatagc
     1201 gcataccttc taatgctgcc agctcactac cttcaatatc aagtttaata aaatcaattt
     1261 tatctatctg ttgttcacta acaaaagcat ctaatgttgt tgcttgtaca gtaaattgtt
     1321 gccctccggt atttaaaatg cttaccgcac ctaaattttg ttggctttcg ctgtaaaata
     1381 agtttaagga attattgtga ttcgaaacgg ccttgtcaaa ccaaataata tttaatccat
     1441 tcaggtgcgc attgtttagt aatgccttgc tgttaatttt aaaaggttcg aatgcatata
     1501 ctttaccttg caaacatact ttagcagcat ttaaagcgta aagaccaatg tttgcaccta
     1561 tatcaataaa aacactattg ggttttagtt ggcttttaaa atatgcgagc tccggttttt
     1621 cgtaactacg caaaaagtaa atattctgtg caatccaatc gtttaagtgc agctgaaata
     1681 tgataccctg atgtttcgtt ttaactacca catttttaaa aagcgaataa ggctttatta
     1741 cctttttata caatccaaaa taataatttc ggaaggcagg tatggccatt ataattcgta
     1801 aaaaataagc gataaaatac ttaacaacaa tcatggctca atactacaac ataacttgac
     1861 ttagataaat atcattgttg cactcattta attcgtagta aatgcatgtg gctgtttaag
     1921 ccgtttaata tgttttattt gcaactttaa taaattattt cttgtgaaag taacactggt
     1981 agtagccgta agtagtaatt gggtaattgg ccaaaacaat aagctattat ggcatttgcc
     2041 taatgactta aggcatttta aaaatataac aacaggtggc accataatta tgggtcgaaa
     2101 aacatttgaa agcattggta agccattgcc aaaccgtgta accattgtta taagtacaca
     2161 aaaagatttt gatgccaaag gcgctattgt ggttaattcg cttgaactgg ctctggaaaa
     2221 atgcaagcat aaagacgaag tttttattgt tggtggtggc gaaatatata aagttgcttt
     2281 gccgatagcg caacaaattt atctaacaca agtacagcaa agttttgatg gtgatacgta
     2341 cttccccgaa attgatttta gccaatgggt tgttacaaaa tccgaaagtt tcgatgttga
     2401 tgataaacat ccctttgcat ataaattcat tacattaatc cgcaagtaac tattttactt
     2461 caaactcagt tcttaattta atatcggctg acgatgcgcc aattaaaact tcaaaaaaac
     2521 cgggttcgac aacaggtttt aagttatcgt ctaaaacggt aagcatgtgc ggcaaaattt
     2581 taaaagttac ttttttactt tcattaggtt gcaattgcaa tcttttaaag cctttcaatg
     2641 ccataaccgg ttgtgcatag ctggctacct tatctttaat ataaagttga attacttcgt
     2701 cagcctttac aatgccggta ttggttatac tgcatgatac agtaaggttt tcatcagatt
     2761 ttataattgc ttttgataac tgcaggttgc tgtatgaaaa tgttgaatag cttaaaccat
     2821 aaccgaacgg aaataaaggt ttgccactta ggtctgtata atcattgccg cgtcctgttg
     2881 gcttatgatt atatactaat ggtaactgcg cttcgctaac cggccaggta aaaggtaatt
     2941 tgcccgatgg agaagtacca tacagtacag atgcaattgc atgcccgcct tgctgccccg
     3001 gataccatac ttgcattact gcatcagctt tttgtagcca agacatggta actgcactac
     3061 cacctaccaa taaaaccact acgggtttct tcaattcaaa aagtttatcc aataaagcct
     3121 cttgcatgcc aggcaattgc
//
LOCUS       DAOMAA010000110         3130 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_24155_length_3130_cov_3.368455, whole genome shotgun sequence.
ACCESSION   DAOMAA010000110 DAOMAA010000000
VERSION     DAOMAA010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24155_length_3130_cov_3.368455"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="PLO59_02270"
     CDS             complement(<1..499)
                     /locus_tag="PLO59_02270"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010189611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQU99951.1"
                     /translation="MKKGLFIIILVYTTMQAFAQGIDKPRYQIVTHRAGNYLGTIQIE
                     LFPLITPLATANFDSLVNAQAYDSTAFHRVVPGFVIQGGDPNSINGPVSTWGMGNPNQ
                     PNVNAEFNVVQHIRGRMGAARDVDTNSANSQFYFCVAPALFLDGNYTVYGQVTSGMDV
                     VDTIVL"
     gene            complement(508..2928)
                     /locus_tag="PLO59_02275"
     CDS             complement(508..2928)
                     /locus_tag="PLO59_02275"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HQU99952.1"
                     /translation="MKIILKILLWITGICAAVFMALTLYIFFNKQYFIDQFTALLSKQ
                     LQTNVSIGNLSFSFIHAFPSAGITLEEVMLTDSLFNLHGKPLLKASKLYISCSIPLLL
                     KKQLTLNELKIEDAQVYLFKHKDGYSNIPKLKTAAQVKNNTSFFLNRIKLVHTEVCIV
                     NDSTHKSFKFTAEQAAVIMQQGLRYVSFEISGTLNVNGLAFNTANGPFLKNANCQING
                     KLINDKTENKLWILPGSEIIANQNPIMLSGYVYSKSTQATSIRLISKNIGYQWASAVL
                     NQKLNTKIKPYAFNGAVNCNVLLNMVSGYTHAQIKIRPVSEISLAINGVGIKAHEFVF
                     SFNNRFNKNAAISDNNSIIQIKNIKATTEGIPLNGYATLFNLSKPFIQTVLSGLIELG
                     KPNQLQAPNLIFTQGSLKYSIDYKGSVSPFYNAQAGHVNGNLLAAIEIGNVSFINKKN
                     GYRFTSLNSNIVIENNDLVVDKMTCAINGNTFQIAGNLKAVVPFLFDNSKALQVVADI
                     NAPLLNIDSLWHKDDVATAKSNNTNQPKYILGMTIEALLDRLNVQAKLTVDKIVFGPI
                     QTNKATGNFEINDSQIGLKNLMFNEGNGVINATVLVKTDVQKINIDASVGGVQVDKFM
                     ESNNDFGQDKIVHQQVEGNLNAILKYEVQLNKQFKVMPATAHGTLDFELQNGRIKNMA
                     GLSKVADFVFKHKNLSDITFQDIVQHATITGNNINIQQLDIASSVLSMRINGVYSLNQ
                     QTDLSIQVPLKNLSRKNTVYKMTIEDLQNYSGANVYFRAYNKDDGSMHIAYDPLKKFR
                     KKSRKNTN"
BASE COUNT          919 a          583 c          536 g         1092 t
ORIGIN      
        1 acaatacaat ggtgtcaact acatccatac ccgaagtaac ctgcccatat acggtataat
       61 tgccatctaa aaacaaagcc ggtgctacac aaaaataaaa ttgcgagttg gcgctattgg
      121 tatcaacatc gcgtgcagca cccatgcggc cgcgtatatg ttgtaccaca ttaaactcgg
      181 catttacatt gggttgattg gggttgccca tgccccaggt tgaaaccggc ccgttaatag
      241 agtttgggtc gccaccttga attacaaagc cgggtaccac acggtgaaat gctgtgctat
      301 cgtatgcctg tgcattaacc aaactgtcga aattggctgt ggctaacgga gttatcagtg
      361 gaaataattc tatttgaatt gtgcccaaat aattgcctgc cctatgtgtt actatttgat
      421 agcgtggctt atcaatacct tgtgcaaaag cttgcatggt ggtataaacc agtataatta
      481 tgaataatcc ttttttcatg ctgttattta gtttgtgttt ttacgagatt tttttctgaa
      541 cttttttagt ggatcataag caatatgcat cgagccatca tccttattgt atgcccgaaa
      601 gtaaacatta gcgcccgaat aattttgcaa atcttcaatc gtcattttgt aaacggtatt
      661 ttttctgctt aagtttttta acggaacctg tatgctcaaa tcagtttgtt gatttaggct
      721 ataaactccg tttatacgca tgcttaatac agagcttgca atatcaagtt gttgtatgtt
      781 tatattatta ccggtaatgg tagcatgttg tacaatatcc tgaaaagtta tatccgacaa
      841 gtttttatgc ttaaaaacaa aatccgctac tttacttaat ccggccatgt tttttatccg
      901 gccgttttgc aactcaaagt ctaatgtgcc atgtgcggta gcgggcatta ctttaaattg
      961 tttgttaagc tgtacttcat attttaagat ggcatttaag ttgccttcaa cctgttgatg
     1021 cacaattttg tcttgcccaa aatcattatt actttccata aacttatcta cctgcacccc
     1081 gcctacactc gcatcaatat taattttttg cacatctgtt tttacaagta cggttgcatt
     1141 tataacaccg ttaccttcgt taaacatcaa attttttaaa cctatctggc tgtcatttat
     1201 ctcaaagttg cctgtggctt tattggtttg tatggggcca aacacaattt tatcaaccgt
     1261 tagttttgct tgtacattta agcgatctaa caacgcttct attgtcattc ccaaaatata
     1321 tttgggctgg ttggtattat tgctttttgc tgttgccaca tcgtccttat gccacaacga
     1381 atcaatatta agcagtggtg cattaatatc agcaaccact tgcagtgctt tactattgtc
     1441 gaacagaaac ggtacaacag cctttaagtt tccggcaatc tgaaatgtat ttccattaat
     1501 ggcacacgtc attttatcta caaccaaatc attgttttca ataacaatgt tgctattaag
     1561 tgatgtaaaa cggtacccat tttttttatt aataaatgat acgtttccta tttcaatggc
     1621 tgcaagcagg tttccattaa catggcctgc ttgtgcatta taaaatgggc ttacactgcc
     1681 tttataatct atactatact ttaaactgcc ctgcgtaaaa attaaatttg gcgcttgcag
     1741 ttgattgggt ttgccaagtt ctatcaggcc acttaaaaca gtttgtatga atggtttcga
     1801 taaattaaat agcgttgcat aaccgttaag cggtatgcct tctgttgttg cttttatatt
     1861 ttttatttga ataatactgt tgttatcgct tatggctgca tttttattga aacgattgtt
     1921 aaatgaaaat acaaattcat gtgccttaat gcctacaccg ttaattgcca aacttatttc
     1981 tgatacaggc cttattttaa tttgagcatg ggtataacct gaaaccatat ttaaaagcac
     2041 attacaattt accgctccgt taaaagcata gggttttatt tttgtgttta gcttttggtt
     2101 tagtacagca gaagcccatt gatagcctat gtttttacta attaaacgta tgcttgtggc
     2161 ttgggtgctt ttactataaa cataacccga taacataatt gggttttgat tagctataat
     2221 ttctgatccg ggtaaaatcc acaacttgtt ttcagtttta tcattaatca gcttaccatt
     2281 tatctgacaa tttgcatttt ttaaaaacgg tccattagcg gtgttaaaag ccaagccatt
     2341 tacatttagt gttccgctta tctcaaaact tacgtaccgt aacccttgct gcattattac
     2401 ggcagcttgc tcagcagtaa acttaaacga tttatgagtc gagtcgttta caatacaaac
     2461 ctccgtatgc accaacttta tccgattcag aaaaaaggaa gtgttgtttt ttacttgtgc
     2521 tgctgtttta agtttgggta tattactgta gccatcttta tgcttaaaca aatacacctg
     2581 agcatcttct atttttaatt catttaatgt aagttgtttt ttaagtaata gtggtatgct
     2641 gcaggatata taaagtttgc ttgcttttaa tagcggctta ccatgcagat taaacaaact
     2701 atcggtaagc ataacttctt ctaatgttat gccggcactt gggaaagcat gtatgaagct
     2761 aaaactcaag ttgccaatac taacattggt ttgcagttgc ttacttagca atgctgtaaa
     2821 ctgatcaata aaatattgct tattaaaaaa aatataaagc gttaatgcca taaaaacagc
     2881 agcacaaata cctgtaatcc aaagcagaat ttttaatata attttcaaac ggggtgctta
     2941 tttattactt gccgtgtaaa taaaaaacaa aataacaaac aagttgcctg tggtattgta
     3001 aattagctgc ccaaattttt gaagccttga gtatatcagc cacacagcaa atagcaatca
     3061 ataatcaaat taatgagttt attaaacgca aaggtttaat gagtacgccc aaaggaaggc
     3121 gtttgtttga
//
LOCUS       DAOMAA010000111         3115 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_24378_length_3115_cov_3.460131, whole genome shotgun sequence.
ACCESSION   DAOMAA010000111 DAOMAA010000000
VERSION     DAOMAA010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24378_length_3115_cov_3.460131"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="PLO59_02280"
     CDS             <1..515
                     /locus_tag="PLO59_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002980628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HQU99953.1"
                     /translation="YDRVNALLSFGAVKSFGISSSLRHICASCVPANIPFFKYEWKKE
                     VWNLIFVFGIFLGGVIAINLLSNPNPVEVNPKLATELAGYGITNFNNLVPKDIVNWQS
                     LFTLKGFLLMVVGGFLVGFGTRYAGGCPSGHTIMGLSNLQLPSLIATISFMLGGFFMA
                     NMILPIILSL"
     gene            527..1027
                     /locus_tag="PLO59_02285"
     CDS             527..1027
                     /locus_tag="PLO59_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HQU99954.1"
                     /translation="MQQNSGAEKDLQLRELDAMCVNESQLQHKWYYNLKYLIIGVLFG
                     IVFVKAEIVSWFRIQEMFRLQSFHMYGIIGSAVVVGMFSVFLIKKFSIKTIYNEPIKI
                     SPKEFNKGQIYGGLIFGFGWAITGACPGPLFSQIGTGASVIGITLLSAIAGTWVYGFL
                     REKLPH"
     gene            1560..1937
                     /locus_tag="PLO59_02290"
     CDS             1560..1937
                     /locus_tag="PLO59_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99955.1"
                     /translation="MKPPADGKMETFTEWKKSEVSFDDNTNATIEYRIALVAKKGLGC
                     HYELEVKNTSDQKLNIKMKSNYYDKLVKSHFGDEIKETLKPGKTVSGRFVGQGCKKEI
                     GVAKTDYEHCIACDLTLSIYVTK"
     gene            2163..>3115
                     /locus_tag="PLO59_02295"
     CDS             2163..>3115
                     /locus_tag="PLO59_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020527549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase,
                     adenosylcobalamin-dependent"
                     /protein_id="HQU99956.1"
                     /translation="MTVNFKSKHQRTMLANEKVTTTKKSKTYFSYKEALAAALAYFNG
                     DELAATTWLKKYAIKLSETQWIETTPDAMHRRMAIQFARIESKYTDKNQTDKTVLSYY
                     GQNRLVLTEDTIFELFKKFKYVIPQGSVMSALGNTYIIASLSNCIVLPEIYDSYGGIF
                     FADQQLAQLFKRRCGTGLDLSTIRPAGMSVSNAAGSTTGAVSFMERFSNTTREVAQNG
                     RRGALMITLDVAHPDIESFITIKQDLLKVTGANISVRLSDEFMHAVKNNTEFTLRFPI
                     DSKTPKIKRTIHARSLWDSIVKCAHHTAEPGLIFWDRQHHYS"
BASE COUNT         1039 a          530 c          571 g          975 t
ORIGIN      
        1 aatacgatcg ggtcaatgct ttgttgtcct tcggtgccgt aaaatcgttt ggtattagtt
       61 cttcactacg gcatatatgt gcttcgtgtg tacctgccaa tattccattc tttaaatacg
      121 aatggaaaaa agaagtatgg aatttaattt ttgtatttgg aattttctta ggcggagtaa
      181 tagccatcaa tcttttatca aatccaaatc ctgttgaagt taatccaaaa ttagccacag
      241 aattggcagg ttacggcatt acaaatttca acaaccttgt tccgaaagac atcgtaaatt
      301 ggcaatcgct atttacttta aaaggatttt tattgatggt tgtgggaggc tttttagttg
      361 gtttcggaac acgttatgca ggtggttgcc caagcggaca taccattatg ggattatcta
      421 acttacaact accgtcattg attgctacaa tcagttttat gcttggcggt ttctttatgg
      481 ctaatatgat tttacctatt attctttcac tttaatcaaa acaacaatgc aacaaaattc
      541 aggcgcagaa aaagatttac aacttcgtga gttagacgca atgtgtgtaa atgaaagcca
      601 attgcaacac aaatggtatt ataatttgaa atatttaatt atcggggttt tatttggcat
      661 cgtattcgtt aaggccgaaa tagtaagttg gtttcgcata caggaaatgt ttcgtttgca
      721 gtcgtttcac atgtatggca taatcggaag tgctgttgtt gttggtatgt tttccgtttt
      781 tctgattaag aaatttagca ttaaaaccat ttataatgaa ccgattaaaa tttcacctaa
      841 agaatttaat aaaggtcaaa tctatggtgg cctaatcttc ggctttggtt gggcaattac
      901 gggagcttgc cccggacctt tgttttcaca aatcggaaca ggcgcaagcg ttatcggaat
      961 aacgctattg agtgccatag caggcacttg ggtttacggc tttttaagag aaaaacttcc
     1021 tcattaaaac atatctatgg attgtattaa aattgctgaa acgtgcaata tggatttcta
     1081 tagaccaaac ttcacggcca caacaacatt acaatgttag ctttagtgtt tcttaaaatt
     1141 tatcaatcaa tttgagtagt tgtgcatcac atgcattgca ttggcagcat aatgctgcca
     1201 atgctcaaat atcaaagcgc taaacagcct tgcgttttat aatatctttc aagcattgca
     1261 ctttcatgct tttagttatt catttttttt tgatggtata aaaaagatgt tccaaacgcg
     1321 acaaaaatta aaatcgcaga atgctcatgt agcacacatt aaccattatc ttataataac
     1381 aattggcaaa tgacatacat atgcgagaac atattaacct ttattgtgcc tgtagcaaga
     1441 acaaatcatt aaaatagaaa ttataaacca attaaaacta taaacatgaa aaaaacattt
     1501 ttagcaatcg ttttactaca acattagcat taagtttaaa cgcacaagtt gtatcgggtt
     1561 tgaaaccacc ggcagatggt aaaatggaaa catttaccga atggaaaaaa tctgaagtta
     1621 gttttgatga taataccaat gcaacaattg agtacagaat tgccttagta gcaaagaaag
     1681 gattgggatg ccattatgaa ctggaagtaa aaaatacttc tgaccaaaaa cttaatatta
     1741 aaatgaaatc aaactactat gataaactgg taaagagtca ctttggtgac gagataaagg
     1801 aaacacttaa acctgggaaa acagtttcag gacgatttgt tggtcaaggt tgtaaaaaag
     1861 aaattggtgt tgcgaaaact gactacgaac attgcattgc atgcgattta acgctttcga
     1921 tatacgtaac taaatagcca tattaaataa gtgcattata gtgcttgtta gatgtatttg
     1981 cataaattat ttcttgaatg ccagcttttt aatttcaggc agttaagcct aattttttaa
     2041 aaatatttag agcagttgta taatttacaa acagtttgtg tttgcaaaaa aacatgtaat
     2101 atgagttagc tcatttttat gcccttcgta ttggtttagt attgtgtttt acaatttaaa
     2161 atttgacagt taatttcaaa agtaaacacc aaagaaccat gctggctaac gaaaaagtta
     2221 caactacaaa aaaatcaaaa acttattttt cttataaaga agcacttgct gctgccctcg
     2281 cctattttaa tggcgatgaa ttggcagcta caacctggtt aaaaaaatat gcaataaaac
     2341 tcagcgaaac gcaatggatt gaaaccacac cggatgctat gcacaggcgt atggctattc
     2401 aatttgcaag aatcgaatca aaatacaccg ataaaaatca aacagacaaa accgtacttt
     2461 cttattacgg ccaaaaccgt ttggtattaa ctgaagatac aatatttgag ttgtttaaaa
     2521 aattcaagta tgtaattccg caaggtagtg taatgtcggc cttgggcaat acgtatataa
     2581 ttgcatcatt atcaaactgc atcgtgttgc ccgaaattta tgattcatac ggaggaatat
     2641 tttttgctga ccaacagctt gcacaactct ttaaaagacg ctgtggcacc ggattagatt
     2701 tatcaaccat acgtccggca ggcatgtcgg tttcaaatgc tgccggcagc acaacaggag
     2761 cggtttcatt tatggagcgt tttagtaaca ccacgcgtga agttgctcaa aatggcagac
     2821 ggggtgcgct aatgattact ttggatgtag cacatcccga tatcgaatcg tttataacta
     2881 ttaagcagga tttgcttaaa gttaccgggg ccaatatttc ggttcgttta tcggatgagt
     2941 ttatgcatgc cgtaaaaaac aatactgaat ttactttacg atttccaatt gatagtaaaa
     3001 cacccaaaat taaacgcact atacatgcac gaagtttatg ggatagcatt gtaaagtgcg
     3061 cacaccatac agccgaaccc ggtttgatat tttgggacag acaacaccac tattc
//
LOCUS       DAOMAA010000112         3106 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_24534_length_3106_cov_3.045887, whole genome shotgun sequence.
ACCESSION   DAOMAA010000112 DAOMAA010000000
VERSION     DAOMAA010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24534_length_3106_cov_3.045887"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="PLO59_02300"
     CDS             complement(<1..557)
                     /locus_tag="PLO59_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HQU99957.1"
                     /translation="MHKSDFDLDYQNQNIESKIIAAFERIAQAFRVLLWQESKVSGLS
                     PIQIQILIFIRHHDAEKCKVSYLANEFNMTKATISDAIKVLEEKELISKVVEKNDIRS
                     YVIKLTNKGKTITNRTALFTTEIRNPIDKLSTNDKTGLLLNLLDIIKHLNQTGVITVQ
                     RMCLTCSHHQNTKKQTHFCKLLNQQ"
     gene            complement(557..820)
                     /locus_tag="PLO59_02305"
     CDS             complement(557..820)
                     /locus_tag="PLO59_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014034077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2024 family protein"
                     /protein_id="HQU99958.1"
                     /translation="MQVAVWDTYVTKKDGSVMHFDIIAPVEIKDTLVIYNYGKAYLKL
                     KKQEGQPLTSKQCRFCHIETIKPAWETDIKQKGYYIYEMENCK"
     gene            complement(968..1201)
                     /locus_tag="PLO59_02310"
     CDS             complement(968..1201)
                     /locus_tag="PLO59_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013306846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HQU99959.1"
                     /translation="MAKSVFYHAGCPVCVSAEHDIINLLGTEQVEVVHFGTDKTRIAE
                     AEKAGVKSVPALVTPNGNVLHINFGASMADVKG"
     gene            complement(1574..2050)
                     /locus_tag="PLO59_02315"
     CDS             complement(1574..2050)
                     /locus_tag="PLO59_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQU99960.1"
                     /translation="MITQKITPCLWVEKDAKAVVDYYLSIFKDGKLIDYRLFDNMPAQ
                     DGHDNASSFETAVMQIAGMEFSILAAGPLFKFNEAISLVINCKDQAEVDYYWQALTIN
                     GGIEGPCGWCKDKYGLWWQVVPVEYFELIHSNDIDVSRKALINTFTQKKIILSELK"
     gene            complement(2047..2556)
                     /locus_tag="PLO59_02320"
     CDS             complement(2047..2556)
                     /locus_tag="PLO59_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018687866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HQU99961.1"
                     /translation="MNKPLFDFVADKANNTLVIKREFDAPRQMVWDCYTKQEYLDQWF
                     APKPFTTKTKSMDFSNGGHWHYAMIDPDGNHYWGYTTYNNIKPIDTYQTEDAFSNEAG
                     EINTELPQAKWEVTFKDFNALTIVETIVYYNSLQDLETIINMGMQEGMKATLEKLDEL
                     LLSLKNETR"
     gene            complement(2569..2730)
                     /locus_tag="PLO59_02325"
     CDS             complement(2569..2730)
                     /locus_tag="PLO59_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99962.1"
                     /translation="MFGCVTPIPFILTKVTITIYNESLERMNESLKYGMEQGFTTTLL
                     QLQNLFSKF"
     gene            complement(2776..3096)
                     /locus_tag="PLO59_02330"
     CDS             complement(2776..3096)
                     /locus_tag="PLO59_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HQU99963.1"
                     /translation="MRRDIFQAIADPTRRAIIALIAMQAMTPNAIAENFNTTRQAVSK
                     HLRILTECELVKQEFQGREIYYSLEIEKMKEIDKWLEQYRKIWESRFNQLDQLLIKIK
                     KQKK"
BASE COUNT          863 a          542 c          534 g         1167 t
ORIGIN      
        1 aattgttgat tgagcaattt acaaaaatgg gtttgttttt ttgtgttttg atgatggctg
       61 caggttaagc acatccgttg caccgttatt acacctgttt gatttaagtg cttaattatg
      121 tctaataaat ttagcaacaa gccggtttta tcgttggtgc ttagtttatc aataggattg
      181 cgtatttcag tcgtaaacaa agccgttcgg ttagttatgg ttttaccttt attagtaagc
      241 ttaataacat aactccgtat gtcgttcttt tcaacaacct tcgaaatcaa ttccttctct
      301 tcaagtactt taattgcatc gcttatggtg gcctttgtca tattaaactc attggccagg
      361 taactcactt tgcatttttc agcatcatga tgtcgaataa aaattaatat ctgtatttgt
      421 atgggactaa ggcctgatac tttgctttcc tgccaaagca ataccctaaa agcctgtgct
      481 attctttcga aagcagctat tattttgctt tcaatatttt ggttttgata atctaaatca
      541 aagtctgatt tgtgcattac ttacaatttt ccatctcgta aatataataa cctttttgtt
      601 tgatatcggt ttcccatgct ggttttatgg tttcgatatg acaaaacctg cactgctttg
      661 atgtaagtgg ttgcccttct tgttttttta attttaaata ggctttacca taattataaa
      721 taaccaaggt gtcttttatt tctacaggag caataatatc aaagtgcatt actgaaccgt
      781 cttttttggt tacataggta tcccaaactg ctacttgcat ttttttattg attttgattt
      841 gcgcctgtgt tgttagtatt aaaaacaaag cacaaactgt gtttattaat ttcattgttt
      901 aaaaattgtg tgaagtaata attgcaagct cacagtgtgg attaagctac actgtgagct
      961 tgcagtttta accctttaca tcggccatgg atgcaccaaa gtttatgtgc aatacattgc
     1021 cattaggtgt aaccaatgca ggaaccgact taacgcctgc tttttcggct tcggcaatgc
     1081 gggttttgtc ggtaccaaaa tgtaccactt ctacttgttc cgttcccaat aagttaataa
     1141 tgtcatgctc tgcgcttaca catacaggac agcctgcatg gtagaaaact gattttgcca
     1201 tttttgtttt gtttaaagtt atgcggcatt attgccggca caataatagt aaggaatcct
     1261 aacaaaacaa aatctgtatc catttttcaa taaatattat agtcataata gaattgtttg
     1321 tttgattgag cgtatatcaa aagcttatca tactactagc tcgaaattgt tatgtattaa
     1381 taatgtttgt taatttacct gattccataa tgcaaaaatc acctatgctt taatagtttg
     1441 cagcataagt aaattgcatt cggtttcgaa tatctttact taaagtttca tttaattttt
     1501 ctattgctgc aatttttgta aagcaagtga ttagttgcgt aacgcgtgtt acatttaaaa
     1561 ttgacgttta gatttatttc agttcagata aaattatttt tttctgcgta aatgtattaa
     1621 tcaaagcttt tcgactcaca tcaatatcat tgctgtgaat gagttcaaag tattcaaccg
     1681 gtaccacttg ccaccataag ccatatttat ccttgcacca accgcatggc ccttctatac
     1741 ctccgttaat agttaatgct tgccaataat aatctacttc ggcttggtct ttacaattta
     1801 taaccaatga aatggcttcg ttaaatttaa atagtgggcc agcagctaat atgctaaact
     1861 ccatgcctgc tatttgcata acagcagttt cgaaactgga ggcattgtca tgtccatctt
     1921 gtgccggcat attgtcaaat aagcgatagt caattagttt cccatctttg aatatggaca
     1981 agtagtaatc aactacagct ttcgcatctt tttcaaccca taggcatggc gttatttttt
     2041 gtgtgatcat cgggtttcgt ttttaagtga aagtaataat tcatccagct tttcaagtgt
     2101 agctttcatc ccttcttgca tgcccatgtt tattatggtt tcgaggtctt gtaatgaatt
     2161 gtagtaaact atggtttcaa ctattgttag tgcattaaag tctttaaaag ttacttccca
     2221 tttggcttgt ggtaattcag tattaatctc gcctgcttca ttactaaagg catcttcggt
     2281 ttggtaggtg tcaatgggct ttatattatt ataagtagta tatccccaat agtggttgcc
     2341 gtcagggtca atcatagcat aatgccaatg gccaccgtta ctaaagtcca tagattttgt
     2401 tttggttgta aagggttttg gagcaaacca ttggtctaaa tattcttgtt tagtataaca
     2461 atcccaaacc atttgacgag gcgcatcaaa ttctcttttt ataactaatg tgttgtttgc
     2521 tttatcagca acgaaatcaa ataatggctt attcatgata tttatttttt aaaattttga
     2581 aaataagttt tgcaattgta aaagtgtagt ggtaaagcct tgctccatac catattttaa
     2641 tgattcattc atccgttcaa gcgattcgtt ataaatagtg atagttactt tggttaatat
     2701 gaatgggatt ggtgtaacac acccaaacat tttgagcaac tgcgtttatg gttgcggtta
     2761 taaaaatttg tggactcatt ttttctgttt tttaattttg attaataatt ggtcgagctg
     2821 attgaaacgg ctttcccaaa tttttcggta ttgctctaac cacttatcta tttctttcat
     2881 cttttctatt tcaagcgaat aataaatttc gcgaccctga aattcttgtt ttaccaactc
     2941 acattcggta agtatgcgca ggtgtttcga tactgcctgt cgggtagtat taaaattttc
     3001 ggctattgca tttggtgtca ttgcctgcat ggcaattaat gcaattatgg cgcgtctggt
     3061 tggatcggca attgcctgaa atatgtctct ccgcataaaa aaatat
//
LOCUS       DAOMAA010000113         3098 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_24640_length_3098_cov_2.978968, whole genome shotgun sequence.
ACCESSION   DAOMAA010000113 DAOMAA010000000
VERSION     DAOMAA010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24640_length_3098_cov_2.978968"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1264
                     /locus_tag="PLO59_02335"
     CDS             <1..1264
                     /locus_tag="PLO59_02335"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HQU99964.1"
                     /translation="PEVRNWWSNLFADLMATGIKGIWNDMNEPAVFDIGTLPEDVRHD
                     YDGQPCSHRKAHNVYGMQMARATFHGVKKYLLGKRPFVITRSGYAGLQRYSSVWTGDN
                     IATWEHLWLANVQIQRLNMSGISFAGSDIGGFIDNPDGELYIRWLQMAVFHPFCRTHY
                     AGLEKGANPQEPWTFGHKVEFLARKFITLRYQLLPYLYTTFWQQTNFGTPMVQPIVMF
                     DQADTETHYRMEEFMVGEKLLVCPVSSPDTHTRKMYLPRGRWFNFWDDSIINGGHELT
                     VHAPLEKIPLFVLEGSVIPNYPKMQFVGQKKVDELTLHVYFSKQQHTSYMFEDEGEGY
                     GYKRRDYNIVKFIVNGSKNQFNIFQTITGHFEPGYKWYKIVIHGLPFEANAFYIDGKV
                     HKLSPKNYALGTIKFRVSRHFEEIVLKH"
     gene            complement(1261..>3098)
                     /locus_tag="PLO59_02340"
     CDS             complement(1261..>3098)
                     /locus_tag="PLO59_02340"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQU99965.1"
                     /translation="CTMTKKNIRYIAFCLLLMHQAFAQPAASNYQLAQQYFNTGEYEK
                     ALVYFEKNYDFDPMGTYPDYLKCLEALKAWDKAEKLIKKQIKKTNDAGPLYVDLGGVY
                     AATGDTVKAAEQYDKAIKAIRPDVSQYLQTANKFLDIGNTQKAIETYKQGRLALPGVY
                     PFSFELADAYATQSNLQAMADEYLDIVEIAPQYIPGLQGILQNKIALDYTGSLADVIR
                     QSLLRRIQKSSSRTEYNELLYWLFLQQKDFESAAIQAKALDKRLAERGDRLLTLGRMC
                     VANQDYKTAEICFQYVMDKGRELNNYTTARIELINALNKRITLQSNYTGNDLQKLSAD
                     YNQAISELGITTTTAPLIAAYAHLKAFYLNDIDTAMQMLESLIAMPRLSPMFQAECKL
                     DLGDMLILKNEVWDALLYYGQVDKDYKHDEIGREAKFRTARAYYYQGEFEWAAAQLNI
                     LKAATTQLISNDAMSLALLIQDNSGFESDSNIAPLLIYAHADLLAFQNHNEAALITLD
                     SIVKEYPGHSLTDEVWFKKAKIFLAKAQIDSAVFYLSDIVTNYPDDILADDALFQMAD
                     LQENRLQNKTQAMQLYETLLQKYPGSLFAVDARKRYRTLRGDLLN"
BASE COUNT          892 a          617 c          638 g          951 t
ORIGIN      
        1 ccccgaagtt cgtaactggt ggagtaattt gtttgccgat ttaatggcta caggtataaa
       61 aggtatttgg aacgatatga atgagcctgc cgtttttgat ataggcactt tacccgaaga
      121 cgtaaggcat gactatgatg gccagccttg ctcgcaccgc aaagcacata atgtgtatgg
      181 tatgcaaatg gcgcgtgcaa catttcacgg tgtaaaaaaa tacctgttgg gcaaacgccc
      241 ttttgttatt acacggtcgg ggtatgcagg tttgcaacgc tacagcagtg tttggacggg
      301 cgataacatt gccacctggg aacacttgtg gttagccaat gtgcagatac aacgtttaaa
      361 catgtcgggc atatcatttg ccggcagcga tataggcggc tttattgata accctgatgg
      421 cgagttatat atcaggtggt tacaaatggc tgtatttcat ccgttttgcc gcacacatta
      481 tgcaggatta gaaaaaggcg caaacccgca ggaaccctgg acttttggtc ataaggttga
      541 atttttagca cgcaagttta ttaccttacg ttatcaactg ttaccgtatt tatatactac
      601 attttggcaa caaaccaatt ttggcacacc catggttcag ccaatagtaa tgtttgatca
      661 ggccgacacc gaaacacatt accgtatgga agagtttatg gtgggcgaaa aacttttggt
      721 ttgtccggtt tcatcgcccg atacccatac gcgtaaaatg tatttgcctc gtggcagatg
      781 gtttaacttt tgggacgata gcattattaa tggcggacat gagttaaccg tgcatgcacc
      841 tttagaaaaa ataccacttt ttgttttaga aggcagcgta atacccaatt atcctaaaat
      901 gcaatttgtg ggtcaaaaga aagtagatga acttacgtta cacgtttact tttcgaaaca
      961 acaacatacc agttacatgt ttgaagacga aggcgaaggt tatggatata aaagacgcga
     1021 ctataacatt gttaagttta tagtaaacgg aagtaaaaat caattcaata tttttcaaac
     1081 tataacaggc catttcgaac ccggttataa gtggtataaa atagtaatac atggtttacc
     1141 attcgaagcc aatgcttttt acattgatgg aaaggtgcat aaactatcgc ccaaaaatta
     1201 tgcattgggc acaataaagt ttagggtgtc acgccatttc gaagaaattg tattgaagca
     1261 ttagtttagc aaatcgccac gcaaggtgcg atagcgcttg cgcgcatcaa cagcaaacaa
     1321 actgcccggg tacttttgca ataaagtttc atacagttgc atggcctgtg ttttgttttg
     1381 caatcggttt tcttgcaaat cggccatttg aaataatgca tcatcagcta aaatatcgtc
     1441 aggatagttt gtaactatat ccgacaggta aaaaacagcc gaatcaattt gcgctttagc
     1501 caaaaatatt tttgcttttt taaaccacac ttcatcggtt aaactatggc cggggtattc
     1561 ctttacaatg ctgtctaatg tgattaaggc tgcttcgtta tgattttgaa atgccaataa
     1621 atcggcatgg gcatatatga gtaagggggc tatattgcta tccgattcaa agcctgaatt
     1681 atcttgtatg agtagcgcca aactcatggc atcgttcgaa ataagctgtg tagttgctgc
     1741 ttttaaaata ttgagttgtg ctgcagccca ttcaaattcg ccctggtaat aataagcacg
     1801 tgccgtacga aactttgctt cgcgtcctat ttcatcatgc ttataatctt tatcaacctg
     1861 gccataatat agtagggcat cccatacttc attttttaaa atcaacatat cacccaaatc
     1921 caatttacac tcggcctgaa acataggcga caggcgcggc atggctatta acgattcgag
     1981 catttgcatg gccgtatcaa tatcgtttaa ataaaatgcc tttaaatgtg cataagctgc
     2041 aataagtggg gctgtagtag tagtaatgcc taattcgcta atggcctggt tgtaatcggc
     2101 actaagtttt tgcaaatcgt tgccggtata attgctttga agggtaatac gtttatttaa
     2161 ggcatttatc agctcaatac gtgcagtggt ataattgttt agttcacgtc ctttatccat
     2221 cacatattga aaacatattt cagcggtttt ataatcctga ttggcaacac acatgcgacc
     2281 taaggtaagt agcctgtcgc cacgctcggc caatcgctta tctaatgcct tagcttgaat
     2341 ggctgcactt tcaaaatcct tttgttgcaa aaacaaccaa tacaatagtt cattatactc
     2401 ggtacggctg ctgcttttct gtattctgcg caataacgat tggcgtataa catcggctaa
     2461 tgaacctgta taatctaaag cgattttatt ttgaagaata ccttgcaaac cgggtatgta
     2521 ttgcggggca atttcaacaa tatctaaata ctcatcggcc atagcctgca agttcgattg
     2581 ggtagcatag gcatcggcca actcgaaact aaacggatat acacccggca aagccagtcg
     2641 cccttgctta taggtttcga tagctttttg ggtattacca atatctaaaa acttattggc
     2701 tgtttgtaaa tactggctta catcgggtct gatggcctta atggctttat cgtattgttc
     2761 ggccgcttta accgtatcgc ctgtggctgc gtaaacgcca cctaaatcaa catataaagg
     2821 cccggcatcg ttggttttct taatttgttt cttaatcaat ttttcggctt tatcccaggc
     2881 ttttagtgct tccaaacatt ttaggtaatc gggataggta cccattgggt cgaagtcgta
     2941 atttttttcg aaatacacca aagctttttc atactcgcct gtattaaaat actgttgcgc
     3001 caactgatag ttgcttgctg ccggttgtgc aaaagcttga tgcataagca gcaaacaaaa
     3061 agctatatac ctgatatttt ttttagtcat ggtgcatt
//
LOCUS       DAOMAA010000114         3095 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_24683_length_3095_cov_2.838487, whole genome shotgun sequence.
ACCESSION   DAOMAA010000114 DAOMAA010000000
VERSION     DAOMAA010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24683_length_3095_cov_2.838487"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="PLO59_02345"
     CDS             <1..967
                     /locus_tag="PLO59_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009388529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase small
                     subunit"
                     /protein_id="HQU99966.1"
                     /translation="LLEENKDRFVLFPIKHPAIWEMYKKSEASFWTAEEVDLSADLID
                     WREKLTDNERHFIKHVLAFFAASDGIVNENLAVNFLQEVQLPEARCFYGFQVMIENIH
                     SEMYSLLIDTYISDSAEKHHLLHAIDTVPCVQKKAEWALRWIGKGSFAERLIAFAAVE
                     GIFFSGSFCSIFWLKKRGLMPGLSFSNELISRDEGMHCDFACLLYSMLNEKLPVTTVQ
                     KLIADAVTIEKEFVSDALPVNLIGMNAELMCQYIEFVADRLLVALGASKIYNATNPFD
                     FMDMISIQGKTNFFEKRVSEYKKAGVNTSANNDTNTQGISFNEDF"
     gene            1012..>3095
                     /locus_tag="PLO59_02350"
     CDS             1012..>3095
                     /locus_tag="PLO59_02350"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HQU99967.1"
                     /translation="MYVIKRNGKQENVKFDKITARIQKLCYGLNPDHVNPLTVAQKVV
                     QGVYDGVSTSELDNLAAETAASLTVQHPDYALLASRVAVSNLHKNTNKSFSQTMQVLH
                     RYIDPKTGKNAPLIADDVWEVIEAHADMLDSTIIYDRDFGYDFFGFKTLEKSYLLKVN
                     GKVVERPQHMLMRVAVGIHKNDVAEAIKTYELMSERWFTHATPTLFNAGTPKPQLSSC
                     FLLTMRSDSIDGIYETLHQTAKISQSAGGIGLSIHNVRATGSYIRGTNGTSNGIVPML
                     RVFNDTARYVDQGGGKRKGSFAIYLEPWHADVFDFLDLKKNHGKEEARARDLFYAMWI
                     PDLFMKRVKAEGSWTLFCPNEAPGLADCYGAEFETLYAKYEAEGRGRKTVPARELWLK
                     IMESQIETGTPYMLYKDACNEKSNQKNLGTIKSSNLCTEIMEYTAPDEVAVCNLASIA
                     LPRFVINGKFDHDKLFDITYQVTKNLNKIIDVNYYPVEEARNSNFRHRPIGIGVQGLA
                     DAFILMRYPFESEEAKKLNREIFETIYYASLTASKDLAIKDGSYQTFAGSPISQGIFQ
                     FDMWQVKPSNRWEWDVLREEVKKHGVRNSLLLAPMPTASTSQILGNNECFEPYTSNIY
                     VRRVLSGEFVVVNKHLLNDLIRVGLWNADVRNTLIAANGSIQNIDGMPQELKDLYKTV
                     WEIKQRTVIDMAA"
BASE COUNT          971 a          644 c          637 g          843 t
ORIGIN      
        1 attattagaa gaaaataaag accgctttgt gttattcccc attaagcacc cggccatttg
       61 ggagatgtat aaaaaatcgg aagccagttt ttggactgca gaagaggtgg acctaagtgc
      121 cgacctaatt gactggcgcg aaaaacttac cgataacgaa cgccatttta ttaaacatgt
      181 attagccttt tttgcggcaa gcgatggtat tgtaaatgaa aacctggccg taaatttttt
      241 gcaagaagta caattgcccg aagcacgttg cttttacggc tttcaggtga tgattgaaaa
      301 tatacacagc gaaatgtact cgctgctaat tgacacctac ataagcgaca gtgccgaaaa
      361 acaccatttg ctgcatgcta tcgacactgt accctgtgtg cagaaaaaag ccgagtgggc
      421 tttgcgctgg ataggcaaag gctcctttgc cgaacgttta attgcttttg ctgccgttga
      481 aggcattttc tttagcggtt cgttctgctc catattttgg cttaaaaagc gcggcctaat
      541 gcccggcctt agttttagta atgaattaat tagccgtgac gaaggcatgc actgcgattt
      601 tgcatgcctg ttatacagca tgttaaacga aaaactaccg gtaacaaccg ttcaaaaact
      661 tattgccgat gcagtaacca tcgaaaaaga atttgtaagc gatgccttgc ccgtaaattt
      721 aattggtatg aatgccgaac ttatgtgcca atacattgaa tttgttgccg accgtttatt
      781 ggttgcatta ggggcttcaa aaatttacaa tgccaccaat ccattcgatt ttatggatat
      841 gatttctatt caaggcaaaa ccaatttttt cgaaaaacgg gtttccgaat ataaaaaagc
      901 aggtgttaac acatcagcaa acaacgatac caatacacaa ggcatcagct ttaacgaaga
      961 cttttaatac tacccatcat ttaacctcaa ctcataataa ataaatagcc catgtatgtt
     1021 ataaaaagaa acggcaaaca agagaatgtg aagtttgata aaattactgc acgcattcaa
     1081 aagctttgct acggccttaa tcccgaccat gtaaacccgc ttacagttgc acaaaaagtt
     1141 gtgcaaggtg tttatgatgg tgtttcaacc agtgagttag ataacctggc tgccgaaact
     1201 gctgcttcgc ttacggtgca acaccccgat tatgccctgt tagccagcag agtggccgtt
     1261 agtaatttgc ataaaaacac caacaaaagt tttagccaaa ccatgcaggt gttgcaccgg
     1321 tacatcgacc ctaaaacggg taaaaacgca ccccttattg ccgatgatgt ttgggaagtg
     1381 attgaggcac atgccgatat gttagactct accataatct acgatcgcga ttttggttac
     1441 gatttttttg gtttcaaaac tttagaaaaa tcatacttgc tcaaagttaa tggtaaggta
     1501 gtagaacgcc cgcaacacat gcttatgcgt gtagccgttg gtattcacaa aaacgatgta
     1561 gccgaagcca ttaaaaccta tgaattaatg agcgagcgtt ggtttacaca tgcaacaccc
     1621 acattattta atgcaggtac acccaagccg caactatcat cttgcttttt attaaccatg
     1681 cgcagcgata gcattgatgg catttacgaa accttgcatc aaaccgctaa aatatcgcaa
     1741 agtgccggtg gtattgggct tagcatacat aatgtacgcg ctaccggatc ctatatacgt
     1801 ggtaccaatg gtacttcaaa tggtatcgta cccatgctac gtgtgtttaa cgatactgcc
     1861 cgttatgttg accaaggtgg cggcaaacgc aaaggctctt ttgccattta tttagaaccc
     1921 tggcatgccg atgttttcga ctttttggat ttaaagaaaa atcacggtaa agaagaagca
     1981 cgtgcccgcg atttatttta tgccatgtgg atacccgatt tgtttatgaa acgggtgaaa
     2041 gcagaaggaa gctggacttt attttgcccc aacgaagcac cgggtttagc cgattgctac
     2101 ggagccgaat ttgaaaccct gtatgccaaa tacgaagccg aaggacgtgg ccgcaaaacc
     2161 gtgcctgcac gcgagttgtg gcttaaaatt atggaaagcc aaatagaaac cggcacaccc
     2221 tacatgcttt acaaagatgc ctgcaatgaa aaatcaaatc aaaaaaactt aggcactata
     2281 aaaagctcaa acctgtgtac tgaaattatg gaatacacag cccccgatga agtagctgta
     2341 tgtaacctgg cttcaattgc actgccacgc tttgttataa atggcaaatt tgatcatgat
     2401 aaattgtttg atataaccta tcaggttacc aaaaacctga ataagattat tgatgtaaat
     2461 tactaccctg tagaagaagc gcgcaacagt aattttaggc acaggcctat tggtattggt
     2521 gtacaaggtt tagccgatgc ttttatttta atgcgatatc cgttcgaaag cgaagaagca
     2581 aaaaaactaa accgcgaaat tttcgaaacc atttactatg catcgcttac agccagtaaa
     2641 gatttagcta taaaagatgg ctcttaccaa acttttgccg gttcgcccat ttcgcaaggc
     2701 atatttcagt ttgatatgtg gcaggtaaaa cccagcaacc gttgggagtg ggatgtattg
     2761 cgcgaagaag taaaaaaaca cggtgttcgc aattcactgt tactggcacc aatgcccact
     2821 gcaagcactt cgcaaatact gggtaataac gaatgtttcg agccctatac atctaacatt
     2881 tatgtaagac gtgttttaag tggcgaattt gtagtagtta acaaacacct gctaaacgat
     2941 ttaattcgtg taggactttg gaatgccgat gtacgcaata ccctaattgc agccaatggc
     3001 tctatacaaa acattgacgg tatgccccaa gaattaaaag acttgtataa aaccgtttgg
     3061 gaaattaaac aacgtaccgt aattgatatg gctgc
//
LOCUS       DAOMAA010000115         3083 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_24876_length_3083_cov_2.443197, whole genome shotgun sequence.
ACCESSION   DAOMAA010000115 DAOMAA010000000
VERSION     DAOMAA010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24876_length_3083_cov_2.443197"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3083
                     /locus_tag="PLO59_02355"
     CDS             <1..>3083
                     /locus_tag="PLO59_02355"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HQU99968.1"
                     /translation="KGYSAGGNNIYFDNFNIPEWATTQVVTGAELFYQEGADVGKGST
                     DQVLLGLKVKTRGSLTPLRLDGIAYSVSQSTAWATDVTGSASYFTGGSPLFNTTGGPV
                     SMPVNAIQFPGSAGLLNFGWYAAACAAPLTPVTMALNPGEDNYVWFVCNVAAGAVTGN
                     FIGAELQFAIVRTPVPLSCLAWGQSTSSANVATTPIPVQSFGIKRQVDQAYNIPTMST
                     GTSWAQYNSNDFISAVKSVGENPSIRIDNYLHDVFCAPCLVTGTYCNRFACHPPDYTQ
                     FKPENKGPYMERTTQYTVGEGVRTGSAPYILQAQCGTWPSSNGVAVFIDWNKDGDFDD
                     TYTGAGGPITENYGTKVQVGGVANMILPLVTWAALSSWNINVPAKSDVVADAGDVNQL
                     PHASIGPIFLGNVRLRVREVFASSNIHPFQSYTFGETEDYTVTVLDDCPTPTSNVCKW
                     LGSASNDWGDPSNWCPAKPTFNDVAYIPITSAPFFYPTIKDTVQAGARMINIQNGARL
                     NIDAANNASLRVLDDINIGFGASAGYSSLNVTSSNDVQAYVPGKTTIPPLGTGLATIT
                     PFRTNSQGKTQIQYTQAELASTYGWKVGDRITHLSFEVHSITLAPAAPSSWQNFRIRA
                     FSMTSMPVFPFPLVAGVKVAVEAADVNVASTTVLYGGAGTTLNLNMPNAAGGTEFNGN
                     FYTVTLTTPLVWDGNDLVLSFEYNTQIAPVPLKTYVLYVEATSGFRTLALNFNAPGAT
                     ITGWNIDGQGRYSGGAAAVTGVVSAQRPRIKFRKQRPYQVFPITIGGDIYNNNNLIRT
                     QPYDITPVYSLVGDSGFVAGYSHVIFDPVGRNLANSPGLLAPNPTYYTGVPGLALTAD
                     AAQEITSSRNSSTIFNRLTINKVSAGNFPVRQASATANALGALADSLALANGEFMLNG
                     KEFRLKNGLASHLVTGAGWLRSEDAATGNMNSKFTWAIGTNTGVHSMPFKGGSGANIF
                     DVIINPTSGDLGNVTVATYATAGNNTPYANPALPAPTRVLTMTTNPTIVDATPWT"
BASE COUNT          856 a          676 c          701 g          850 t
ORIGIN      
        1 taaagggtta cagtgccggt ggtaacaata tatattttga taattttaat atacccgagt
       61 gggcaactac acaagttgtt accggtgccg agctttttta ccaggaaggt gccgatgtgg
      121 gtaaaggcag tactgatcag gttttattgg gtttaaaagt taaaaccagg ggtagtttaa
      181 caccactcag gttagatggt attgcttact cagtaagcca aagtacggct tgggcaacgg
      241 atgtaacagg cagtgcatcg tactttacag gtggtagccc gctgtttaat actacgggtg
      301 gccctgttag tatgccggta aatgctatac agtttccggg ttcggcaggc ttattaaatt
      361 ttggctggta tgcagcagct tgtgcagccc ctttaacccc tgttaccatg gcattaaacc
      421 cgggcgaaga taactatgtg tggtttgtct gtaacgttgc tgcaggtgct gttaccggta
      481 attttattgg tgccgagtta cagtttgcta ttgtgcgtac gcccgtgcca ttatcttgct
      541 tagcctgggg ccaaagtacg tcaagtgcca atgtagctac tacacccata ccggtacaaa
      601 gttttggtat taaaaggcaa gttgaccagg catataacat accaaccatg agtacaggta
      661 cttcgtgggc gcaatacaat agcaacgatt ttattagtgc agttaaaagt gttggtgaaa
      721 atccaagtat tcggattgat aattacctgc atgatgtttt ttgtgcaccc tgtttagtaa
      781 ccggtaccta ttgcaaccgc tttgcctgcc atccacccga ttatacacaa tttaagcccg
      841 aaaataaagg cccctatatg gagcgcacca cacaatatac agtgggtgag ggtgtgcgaa
      901 ccggaagcgc accatacata ttacaggcac aatgcggtac ctggcccagt agcaatggag
      961 ttgctgtatt tattgattgg aataaagatg gcgattttga cgatacttat acaggtgctg
     1021 gtggccctat cactgaaaat tatggcacta aagtacaagt aggtggtgtt gccaacatga
     1081 tattgccctt agttacctgg gctgcgctat cgagctggaa cattaatgta cctgcaaaaa
     1141 gtgatgttgt agccgatgcc ggtgatgtaa accaattgcc tcatgcatca ataggcccaa
     1201 tatttttagg taatgtgcgt ttgcgtgtgc gcgaagtttt tgcaagctca aacatacatc
     1261 cgtttcaatc ctacaccttt ggcgaaactg aggactatac cgttaccgta ttagacgatt
     1321 gccctacacc taccagcaat gtttgtaagt ggttaggcag tgccagtaac gattggggcg
     1381 acccatcgaa ctggtgcccg gccaaaccta cttttaatga tgtagcttac ataccgatta
     1441 cttcagcgcc tttcttttac ccaactatta aagataccgt acaggccggt gcccgtatga
     1501 ttaacataca aaacggtgcc cgccttaata ttgatgcggc caacaatgca tcgttacgcg
     1561 tgttagacga tataaatata ggttttggtg catcggcagg ttatagcagc cttaatgtta
     1621 ccagcagtaa tgatgtgcaa gcctatgtgc ccggcaaaac taccatacca ccattaggca
     1681 ccggtttggc aactattaca cctttcagaa caaactcaca aggtaagacg caaatacaat
     1741 atacgcaggc cgaattggct tcaacatacg gttggaaagt aggcgataga atcacacatt
     1801 taagcttcga agtacactcc attaccttag ccccggcagc gccatcatca tggcaaaact
     1861 ttcgtattcg tgcattttca atgacttcaa tgcctgtatt cccctttcca ttagttgcag
     1921 gtgtaaaagt tgcagttgaa gccgctgatg taaacgtagc atcaacaacg gttttgtatg
     1981 gtggggccgg tactacactt aatttgaata tgcccaatgc agcaggaggt actgagttta
     2041 atggtaattt ttataccgtt accttaacca cgcctttggt ttgggatggc aatgacttag
     2101 tgctttcttt cgaatataat acacaaatag ctcctgttcc tttaaaaacg tatgtcttat
     2161 acgttgaagc aacatcggga tttagaacat tagcacttaa ttttaacgca cccggtgcaa
     2221 caattactgg ctggaatatt gacggacaag gccgttattc gggtggtgct gcagcagtaa
     2281 ctggtgtagt atcggcacaa cggccacgta ttaaattccg taaacaaagg ccttatcagg
     2341 tatttccaat aaccataggt ggcgatattt ataacaacaa caatttaata cgtacacagc
     2401 cttacgatat aacaccggtt tacagccttg tgggcgatag cggttttgtg gccggttaca
     2461 gccatgtaat attcgaccct gtgggtcgta acttagccaa ctcgcccggc ttattggcac
     2521 ccaacccaac ttactacaca ggtgtacccg gcttggcatt aacagccgat gccgcacaag
     2581 aaattaccag tagccgtaat tcatcaacca tatttaacag gcttactatt aataaagtaa
     2641 gtgccggtaa tttcccggta aggcaggctt cagcaacagc aaatgcttta ggtgccttag
     2701 ccgattcatt ggcattggcc aatggcgagt ttatgcttaa tggtaaggag tttaggctta
     2761 aaaatggatt ggcatcgcat ttagttacgg gtgcaggctg gctgcgcagc gaggatgcag
     2821 ccaccggtaa tatgaacagt aaatttacct gggctattgg aaccaacacc ggtgtacata
     2881 gtatgccgtt taaaggcggt agtggtgcca atatttttga tgtaataatt aatccaacat
     2941 caggcgattt gggcaatgta acagtagcta cctatgctac tgctggtaac aacacaccat
     3001 atgcaaatcc ggcattaccg gcaccaacaa gggtattaac catgactacc aacccaacca
     3061 ttgttgatgc cacaccctgg act
//
LOCUS       DAOMAA010000116         3075 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_24997_length_3075_cov_2.481126, whole genome shotgun sequence.
ACCESSION   DAOMAA010000116 DAOMAA010000000
VERSION     DAOMAA010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24997_length_3075_cov_2.481126"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="PLO59_02360"
     CDS             <1..729
                     /locus_tag="PLO59_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018472862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HQU99969.1"
                     /translation="ISHNDLENLHDCKVLIRAHGEPPSTYQTALKNNIELIDASCPVV
                     LKLQNRVRTSFDSFDDADGQIVIYGEEGHAEVNGLVGQTKGQAIIVRTEADLEKVDFK
                     QPIHFFSQTTKSSAGFKKMKELIEARVIESKGALTDENLEANDTLCRQVSNREPRMQR
                     FSQEHDVVVFVSGKKSSNGKALYSVCKSVNERSYFISEPNELNASWFVETDSVGICGA
                     TSTPMWLMQDVADKLKLLFVVETA"
     gene            754..3072
                     /locus_tag="PLO59_02365"
     CDS             754..3072
                     /locus_tag="PLO59_02365"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HQU99970.1"
                     /translation="MKSGFLILVAVVFFTTANAQVNKTLKLPFICTPIQLNFSETKVY
                     ITEYITDIKQIDSIVINNKSLQINQTEGTITIKGKTKQPIDVLQIWCKKGLVEIPVFD
                     SEKQHYKLIYKTTNQPKSVAVSGSMNGWNKAASPLKLVNNQYETNFILNPGTYQYRIW
                     EDEKELLDVNNKNLADNGMGGQNNFFVVGHTGIIPVLYCNKPEGNLITISVINEVKTA
                     KVFYDNAVLTYESKNNKIQFKIPAYDTLMHTIRVFAHNGYKRSNDILLPVKNGKVVTN
                     ESDISRSDMHKAIMYFAMVDRFADGNKNNNRPTIDNSILPIANNMGGDIAGITAKIES
                     GYFKQLGINTIWLSPIVQNAEGAWGLWNKGKTSKFSAYHGYWPLCMNNIDNRFGNDAE
                     FNELIHVAHQHQMNVLLDYVAHHVHQNHPLVTEKPEWFTPLYLPDGSMNTEKWDEHRL
                     TTWFDTFLPTWKFADKVVVNALTDTAMYWITKYDIDGFRHDATKHIEENFWRTLTYKF
                     RKYQAQNPNRSLYQIGETYGNPELIGSYISSGKLDAQFDFNLYDAAVNAIAVNETTFE
                     NLNNVLTESLYNYGSHNLMGNITGNQDRARFISYADGSVLFSEDAKLAGWTRTINNRA
                     GGFEKLAMLHAFLLTTPGVPCIYYGDEIGLPGGNDPDNRRMMLFDTLNIEQQKLKHTV
                     EKLAAIRNNNMALTFGDTYILKHDDAMVYVRSYCGQNVLVVLNKKPGKWQLSIPQHLL
                     PSGTNKLNNVQMEIKNNLLQVDATNTFFEVIY"
BASE COUNT         1088 a          540 c          594 g          853 t
ORIGIN      
        1 atatcacata acgatttaga aaacctgcac gattgcaaag tattgatacg agcacatggc
       61 gaaccaccat caacttatca aacagcttta aaaaataata tcgaacttat tgacgcatcc
      121 tgcccagtag ttttaaaatt gcaaaaccgc gtacgaacca gttttgatag ttttgatgat
      181 gcagatgggc aaatagtaat ttatggcgaa gaagggcatg ccgaagttaa tggcttagtg
      241 ggccaaacca aaggacaggc tattattgtt cgaaccgaag ccgatttaga aaaagttgat
      301 tttaaacaac ctattcattt cttttcacaa actacaaaaa gttcggcagg ctttaaaaaa
      361 atgaaagaac ttattgaagc gcgagtaatt gaaagtaaag gcgcacttac tgatgaaaat
      421 ttagaagcca atgatacgtt gtgcagacag gttagtaatc gcgaaccgcg gatgcaacgc
      481 tttagtcagg agcatgacgt ggttgttttt gtaagcggta aaaaaagcag taatggcaaa
      541 gccttataca gcgtttgcaa atcggttaac gagcgctcct attttatatc agaaccaaac
      601 gaattaaatg caagctggtt tgttgaaacc gatagtgtgg gtatctgcgg tgccacatca
      661 acaccaatgt ggttaatgca ggatgtggcc gataaactca aactattgtt tgttgttgaa
      721 actgcctaac tcaaataatt tattttaaat aatatgaaat caggattctt aattttggtt
      781 gcagtagtgt tttttacaac cgctaatgcg caagtaaaca aaacgctaaa gctgcctttt
      841 atttgtacac ccattcaact caactttagc gaaacaaaag tttatattac ggagtacatt
      901 acagatataa aacagattga ttcaattgta atcaacaaca aatcgcttca aattaaccaa
      961 acagaaggaa ccattaccat taagggaaaa acaaaacaac ctatagatgt attacaaatt
     1021 tggtgtaaaa agggtttggt tgaaattccg gtattcgatt ccgaaaaaca gcattacaag
     1081 ttaatttaca aaactacaaa ccaacccaaa tcagttgccg tatcgggcag catgaatggc
     1141 tggaataaag ctgcatcgcc actcaaattg gttaacaacc aatacgaaac gaacttcatc
     1201 cttaatccag gcacatacca ataccgtatt tgggaagacg agaaagaatt gttagatgtg
     1261 aataataaaa atttggcaga caatggaatg ggggggcaaa acaatttttt tgtggtagga
     1321 cataccggca ttattcccgt tttatattgc aataagcccg aaggcaattt aattactatt
     1381 tcagtaatta atgaagttaa aacagccaaa gtattttacg ataatgcggt attgacttat
     1441 gaaagtaaaa acaataaaat tcaatttaaa atacctgctt acgatacgtt aatgcatacc
     1501 atcagagtat ttgctcacaa tggctataaa cgtagtaatg atattttgtt accggtaaaa
     1561 aatggtaaag ttgttaccaa cgaatctgat ataagtcgta gcgatatgca caaagccatt
     1621 atgtattttg ctatggttga cagatttgca gacggaaaca aaaacaacaa cagaccaaca
     1681 attgataaca gcattttacc aatagccaat aatatgggtg gtgatattgc cggtattaca
     1741 gcaaaaattg aatctggata ttttaagcaa ttaggtatta acaccatttg gctttcgcca
     1801 attgtacaaa atgctgaagg tgcctgggga ttatggaata aagggaaaac cagcaagttt
     1861 tcagcctatc atggatactg gccactttgc atgaacaaca ttgataaccg ctttggtaac
     1921 gatgcagaat ttaatgaact gatacatgta gcacatcaac atcagatgaa tgtgctattg
     1981 gattatgtgg cacatcatgt gcatcaaaac catccattgg taaccgaaaa acccgagtgg
     2041 tttacgccat tgtatttgcc tgacggaagt atgaatactg aaaaatggga cgagcatagg
     2101 ctgacaacct ggtttgacac atttttacca acctggaaat ttgctgataa agtagtagta
     2161 aatgcactta ccgatacagc catgtattgg attacaaaat atgatattga tggtttcagg
     2221 cacgatgcaa ccaaacatat tgaggaaaat ttttggcgca cacttacata taaattccgc
     2281 aagtatcagg cacaaaatcc aaatcgtagc ttgtatcaaa ttggcgaaac ctatggtaat
     2341 cccgagttaa taggctccta tataagcagc ggaaaattag atgcacaatt cgattttaat
     2401 ttatacgatg cagctgttaa cgcaattgcc gttaacgaaa ccactttcga gaacttaaac
     2461 aatgtactaa ccgaaagttt atacaattac ggcagtcata acttgatggg caatattacc
     2521 ggcaatcagg acagggcgcg gtttatttct tatgctgatg gttcggtttt attttccgaa
     2581 gatgcaaagt tagcaggctg gaccaggacc ataaacaata gggcaggagg tttcgaaaaa
     2641 ttggcaatgc ttcatgcttt cttacttact acacccggtg tgccttgtat ttattatggc
     2701 gatgaaatag gattacccgg aggtaacgat cctgataaca ggcgcatgat gttatttgac
     2761 acattaaaca ttgaacaaca aaaattaaaa cacaccgttg aaaaactggc tgcaatacgg
     2821 aataataata tggcgcttac ttttggtgat acctatattt taaaacacga tgatgcaatg
     2881 gtttatgtac gcagctattg cggccaaaat gtattggttg tattaaataa aaaaccaggg
     2941 aagtggcaat tatccattcc gcaacattta ttaccatcag gcacaaacaa attaaacaat
     3001 gtgcaaatgg aaataaaaaa caaccttttg caagttgatg caaccaatac gttttttgaa
     3061 gtgatttatt aaatg
//
LOCUS       DAOMAA010000117         3070 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_25085_length_3070_cov_2.802985, whole genome shotgun sequence.
ACCESSION   DAOMAA010000117 DAOMAA010000000
VERSION     DAOMAA010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25085_length_3070_cov_2.802985"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="PLO59_02370"
     CDS             <1..214
                     /locus_tag="PLO59_02370"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU99971.1"
                     /translation="LIGFALRQNAEVSLDLYDIYGNHVANMLKGFKNAGKHMINFDRK
                     HLKRGTYFYKLTSGNYSATKKMVVVN"
     gene            complement(611..1774)
                     /gene="porV"
                     /locus_tag="PLO59_02375"
     CDS             complement(611..1774)
                     /gene="porV"
                     /locus_tag="PLO59_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system outer membrane channel
                     protein PorV"
                     /protein_id="HQU99972.1"
                     /translation="MATSSLNAQDTLNTSQLLGGINTITTAVPFLLICPDSRAGGMGE
                     VGVSTSPDVNSNHWNVAKLPFSSNTGGIGISYTPWLRSLVPDISLAYLSGFKKLKNDQ
                     TISGSLRYFSLGNITFTDVVGNVTGQFNPNEFAIDIAYARKLGEEISGGLTLRYINSN
                     LTGGIEVQGAQTRAGQAVAADLGVYWLHESEVAGKDATITVGGTITNIGNKISYSETG
                     IKDFIPTNMRMGTGLQLELNDYNTISAHLEFSKLLVPTPPIYATDTSGNTFILHGKDP
                     NVGVAKGMFQSFSDAPDGFSEELKEIIWQLGFEYWYDKQFAFRTGYFHESADKGNRQF
                     FTVGVGVRYNIFGLDFAYLIPTQQRNPLENTLRFSLSFDFNKGIKKAKETTTE"
     gene            complement(1962..>3070)
                     /locus_tag="PLO59_02380"
     CDS             complement(1962..>3070)
                     /locus_tag="PLO59_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HQU99973.1"
                     /translation="MVYPKLKVAATQLNGQNINTATDTLKALGTVTIKGEVQDAAGNK
                     LSNYNGIVYPTVYDKMDSLKTLRNDSTGNNQSGNFNYQIQKNILYKGKVSVTNGAFEF
                     TFIVPKDIKYNTGHGRISFYVQNGFEDGSGFNTNIKIGGINTSAVADKLGPEVKLYLN
                     NEKFVFGGLTNNNPVVYALVADSSGINTVGNGIGHDITVTVNDDANKIFNVNDYYQSN
                     LNSYSSGKVIYPLELLPPGRHALKIKVWDIHNNSSDAYTEFTVAESAGMVIDRVLNYP
                     NPFTTNTRFFFEHNLTCQTLLVDIKIFTLSGKLIKNLSQYVDCQGYRLDGIAWDGRDD
                     FGDPIGKGVYIYKLKVTSAEGQSADKIEKLVILR"
BASE COUNT          929 a          549 c          571 g         1021 t
ORIGIN      
        1 gttgataggt tttgcgctaa ggcaaaatgc tgaggttagt ttagatttgt atgatattta
       61 cggaaaccat gttgccaata tgcttaaagg ttttaagaac gcaggtaaac acatgattaa
      121 tttcgacaga aaacacttaa agcgaggcac ttacttttat aagcttacat cgggtaatta
      181 ttcggctact aaaaaaatgg tagtagtaaa ttagttttcg cttcactcaa aatataaagg
      241 ccttcatgca aatagcatta tggccttttt taattcccct atggtttcat tgtttttgat
      301 tgttatactt ttttaagcat aaataaattt tatataaagt gaggtttcta tggttatatg
      361 tgaaattttt atttagctac aggaagccga tgcataagat ttagaacact ttttaaactt
      421 agcacgaaaa caatttttga aataaacaac tgatttaaaa tgaacaacct aataggtgat
      481 ttgaaatatg caatagcatt cgatagtttg cttaaactac atcaggtttt atagcaaaaa
      541 actaaatgtt taattaaaaa aaaagccggc ctctgatcga agccggcttt tggcaattaa
      601 ttttagctac ttactcggtg gtagtttctt tggctttttt aattccttta ttaaaatcga
      661 atgataaaga aaaacgtaat gtgttttcta atggattgcg ttgttgggtg gggattaagt
      721 atgcaaaatc aagtccaaaa atattgtatc gaacgccaac accaacggta aaaaactgac
      781 ggtttccttt gtcggccgat tcatgaaaat agccggtacg gaatgcaaat tgtttatcgt
      841 accagtattc aaaacccaat tgccaaataa tttcttttaa ttcttcgcta aagccatcag
      901 gtgcatcgct aaacgattga aacatacctt ttgcaacacc tacattggga tctttgccat
      961 gcaaaataaa cgtattgccc gatgtgtcgg ttgcgtatat gggaggtgtt ggtaccagta
     1021 gtttactaaa ctctaaatga gccgagattg tattgtaatc gtttaactct aattgtaatc
     1081 cggtacccat gcgcatattg gttggtataa aatcttttat gcccgtttcg ctgtacgaaa
     1141 tcttatttcc aatatttgta attgttccgc caacggttat agtggcatct ttaccggcaa
     1201 cttcactttc gtgtagccaa taaacaccta agtcggcagc aacggcttga cctgcacggg
     1261 tttgcgcacc ttgcacttca atgcctccgg ttaaattcga attaatgtat ctaagcgtta
     1321 atcctcccga aatttcttcg cccaatttac gtgcataagc aatgtcaatt gcaaattcat
     1381 tgggattaaa ctgtccggta acattgccta ctacatcggt aaaagttata ttgcctaatg
     1441 aaaagtatcg taacgaaccg gaaatagttt ggtcgttttt aagtttttta aatccactta
     1501 aatatgccaa actaatatca ggtactaagc tacgtaacca aggtgtatag gaaataccaa
     1561 tacctccggt atttgatgaa aaaggcaact tagccacatt ccagtgattg ctatttacat
     1621 caggcgatgt tgaaacgcca acttcgccca tgccgcctgc acgcgaatcg gggcagataa
     1681 gtaaaaaagg tacagcagta gttatggtat taataccacc taaaagctgg ctggtgttta
     1741 gtgtgtcttg cgcatttagc gaactagttg ccatagtact gcaagcgcaa atggctgtga
     1801 aaatgcgtgc taatgtgtta cttgtcatca aaataggttt aaaataaagg gtggcgaata
     1861 taaaggttgt tttttaaaaa gggttgcaaa atacgcttaa tacatggttt tgtgttaagc
     1921 gataatttgc ggcaaaaatt gttattgcaa atgataattt attaacgaag tattaccagt
     1981 ttttcaattt tatcggcact ttggccttcg gccgatgtta ctttaagttt ataaatgtaa
     2041 acgcctttgc ctattgggtc gccaaagtca tcgcggccat cccaggcaat accatctaat
     2101 cgatagccct ggcaatcaac atattggctc aggtttttaa taagctttcc cgatagggta
     2161 aaaatcttaa tatcaaccag caaagtttgg caagttaggt tgtgttcgaa aaagaaacgt
     2221 gtgttggtag taaatggatt tgggtagtta agtaccctgt ctataaccat accggccgat
     2281 tcggcaacgg taaattcagt ataggcatcg cttgagttgt tatgtatatc ccatactttt
     2341 atttttaatg catgtcggcc ggggggtaat aattcaagcg ggtatattac tttaccacta
     2401 ctataagaat tcaaattact ttgatagtaa tcgtttacat taaaaatttt atttgcatcg
     2461 tcatttaccg tcactgtaat atcgtgccca ataccgttgc caaccgtgtt tataccgctt
     2521 gagtcggcaa ccaatgcata tacaacaggg ttattatttg ttaagccacc aaacacaaat
     2581 ttctcattgt ttaaatacag cttaacctct gggcctaact tatcagcaac agccgaagta
     2641 tttatgccac ctattttaat gttggtatta aatccgctgc catcttcaaa accattttga
     2701 acataaaaac tgatacggcc atggcccgtg ttatatttaa tgtctttagg tacgataaat
     2761 gtaaattcaa atgccccatt ggtaacactt actttacctt tatacaatat gtttttttga
     2821 atttgataat taaagttacc gctttgatta ttaccggtcg aatcgtttct tagtgttttt
     2881 agcgagtcca ttttatcata aacggttgga taaacgatgc cattatagtt tgacagttta
     2941 tttccggcag catcttgtac ttcgccttta atggtaacgg tacctaatgc ttttaatgta
     3001 tcggttgcag tatttatgtt ttgaccattt agttgtgtgg ctgctacttt taatttggga
     3061 tataccattt
//
LOCUS       DAOMAA010000118         3039 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_25586_length_3039_cov_2.444705, whole genome shotgun sequence.
ACCESSION   DAOMAA010000118 DAOMAA010000000
VERSION     DAOMAA010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25586_length_3039_cov_2.444705"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="PLO59_02385"
     CDS             <1..361
                     /locus_tag="PLO59_02385"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HQU99974.1"
                     /translation="QDDILDVYADPEKFGKQIGGDIIANKKTYLLLSALYHANIDQYE
                     QLTDLLHDTKNRYAAYEKISRVTTIYNALGVKEMAAEAVKHYYQLAITHLEAVEMDAP
                     QKQMLYDFGSDMLQREL"
     gene            458..901
                     /locus_tag="PLO59_02390"
     CDS             458..901
                     /locus_tag="PLO59_02390"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013598188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HQU99975.1"
                     /translation="MLTSINTHAYFMREALREAQKAFDADEVPVGAVIVCDEKVIARA
                     HNYTERLTDPTAHAEMQVITAATNYLGAKYLSQCTLYVTLEPCMMCTGAIFWSQLAAI
                     VYGAPDPKRGFLSRQNILLPKTIITGNILADDCSQLLTTFFKNKR"
     gene            990..1406
                     /locus_tag="PLO59_02395"
     CDS             990..1406
                     /locus_tag="PLO59_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004916385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HQU99976.1"
                     /translation="MPYPEQLVAPFRADLTSAGFQELTTPEQVDAVLPTAAGTTLLVI
                     NSVCGCAAGAARPGIKMSLNNAKKPNNLVTVFAGQDLEAVQQARKYTLPYPPSSPSIA
                     LFKDGKLVHFVERHHIEGRTAEMIAENLQSAFDTYC"
     gene            1465..1920
                     /locus_tag="PLO59_02400"
     CDS             1465..1920
                     /locus_tag="PLO59_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQU99977.1"
                     /translation="MYTFIETDYQNPDYVMLVNELDATLALRDGKAHAFYAPHNTSNQ
                     LQVIIVALHNGKPVACGAFKFHKPEVAEIKRMFVLPSHRKKGLATAILAHLEKRCLKQ
                     NYKVCVLETGLNQPEAIALYRKAGYNEIQPFPPYENAPLSICFSKTLLA"
     gene            complement(2008..2952)
                     /locus_tag="PLO59_02405"
     CDS             complement(2008..2952)
                     /locus_tag="PLO59_02405"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU99978.1"
                     /translation="MRKIFVIVIILILQFNSVHSQNIIANGSFEDTVTCQQWLNLSNG
                     LLICIPWFMAMGTPDYFSNSIFLNFCGASTLYPNSILGYQLPRTGSGMAGFELKNMNT
                     EMISSSIQPMIQSHLYFLSYYISLCNNCKYVTDDIGAYLSIDSLNPDSILTLNLSPQV
                     SNPQGNYINDTAGWTLISGYYQAIGGEQYLYIGNFKNAANTTFDTIAGASYWGSYYFL
                     DDVSLIDCTATSIVENTALQFQSLNNPVNGTLQFITATKVNRCFIYDMIGNKISTYNL
                     NQNTNTYTVSVTDLKQGMYLLVIEDIEKRRGMRKFIKQ"
BASE COUNT          979 a          606 c          530 g          924 t
ORIGIN      
        1 acaagatgat attttagatg tttatgccga ccccgaaaaa tttggcaaac aaataggtgg
       61 cgatattatt gcaaacaaaa aaacctactt gctcctatcg gcattatacc atgccaatat
      121 tgaccagtac gaacaactta ccgacctttt gcatgatacc aaaaaccgtt atgccgctta
      181 tgaaaaaata agccgtgtaa ccactatata caatgctttg ggtgtaaaag aaatggctgc
      241 cgaagccgtt aaacattatt accaattagc cataacacat ttagaggctg ttgaaatgga
      301 tgcaccacaa aaacaaatgt tatacgactt tggcagcgat atgctgcagc gcgaactgta
      361 aataaatttc gttgcataca tgtatagttt gcttattaca tgtaacaaat aaaaacacaa
      421 gcattatctg aacctgttca attactaata tatcaaaatg ctaacctcaa taaatacaca
      481 tgcatacttt atgcgcgaag cattacgcga agcgcaaaaa gcatttgatg ccgatgaagt
      541 gcctgtaggt gctgtaattg tttgcgatga aaaagtaatt gcccgtgccc ataactatac
      601 cgaacgccta accgacccca cagcacatgc cgaaatgcaa gtaataaccg ctgccaccaa
      661 ttacctgggt gcaaaatatt taagccaatg taccttgtac gttacgcttg agccatgtat
      721 gatgtgcacc ggtgccatat tctggagtca attagcagct attgtgtatg gcgcccccga
      781 ccccaaacgc ggttttttat cacgtcaaaa tattttatta cctaaaacga taataaccgg
      841 taatatactg gccgatgatt gcagccaact attaactacg ttttttaaaa acaagcgtta
      901 acaagggttt aattgtaatt tcggtttgta aaagcttaac cgcctatatt tgtaagttat
      961 aaattttaaa ctttaaaaat catcatatca tgccttatcc cgagcaatta gttgccccat
     1021 tcagagccga tttaacatcg gcaggttttc aagaacttac cacacctgaa caagtggatg
     1081 ccgttttacc aacagcagcc ggtactactt tattagtaat aaactccgta tgcggttgtg
     1141 ctgccggtgc tgcacgcccg ggtattaaaa tgagtttaaa caatgccaaa aagcctaata
     1201 atctggttac tgtttttgcc gggcaagatt tggaagctgt gcaacaagca cgcaaatata
     1261 cattgcctta cccgccttca tcgcccagca ttgccttgtt taaagatggc aaactggtgc
     1321 actttgttga gcgccatcat attgaaggcc gtactgccga aatgattgcc gaaaatttgc
     1381 agtcggcatt tgacacctac tgctaaaaaa caaacagagc tgcctacaag cagctttttt
     1441 tatgcttata aaaactaaag tttcatgtac actttcattg aaaccgatta tcaaaaccca
     1501 gactacgtaa tgctggttaa cgaacttgat gcaacgttag ccttacgcga tggcaaagca
     1561 catgcatttt acgcaccgca caatacttcc aatcagttgc aggtaataat tgtggcattg
     1621 cataacggca aaccggtggc atgtggtgca tttaaatttc acaaacccga agtagccgaa
     1681 ataaaacgca tgtttgtttt accatcgcat cgcaaaaaag gcctggcaac ggctatttta
     1741 gcccatcttg aaaaacgatg cttaaaacaa aactacaaag tttgtgtatt agaaacgggc
     1801 cttaatcaac ccgaagccat tgccctgtat cgcaaagcag gctataatga aatacagccg
     1861 tttccgcctt acgaaaatgc accgttaagc atctgtttta gtaaaacact tttagcctaa
     1921 taataccggt aattcaatta tctgtttgac aaagtgacaa ttgtctgaac catgatttat
     1981 aggattaaag gattttagga ttatctctta ctgttttata aactttctca ttccccttcg
     2041 tttttctata tcctctatga ccaacaggta catgccctgt tttaaatctg taacacttac
     2101 tgtataggtg ttagtgtttt gatttaaatt ataagttgaa attttattgc caatcatatc
     2161 ataaataaaa cacctgttta cctttgttgc tgttataaat tgcaatgttc cattaacggg
     2221 gttgtttaat gattgaaatt gtaaagcagt attttctact atgctcgttg ctgtacaatc
     2281 aatcaggctt acatcatcta aaaaataata actgccccaa tagcttgctc cagcaatagt
     2341 atcgaaagtt gtgttcgcag catttttaaa gttgcctatg tataaatatt gctctccccc
     2401 tatagcctga taataaccgg atattaatgt ccaacccgct gtatcattta tatagttgcc
     2461 ttgcgggttt gaaacttgtg gcgatagatt taacgtcaat attgaatcag gatttaaact
     2521 gtcaattgat aagtacgctc caatatcatc agtaacgtac ttacaattgt tgcatagtga
     2581 aatataatat gacagaaaat aaagatgact ttgtatcatt ggttgaatgg atgaggaaat
     2641 catttctgta ttcatatttt ttaactcaaa tcctgccatg cctgaccctg ttcgtggtaa
     2701 ttgatatcct aaaattgaat tagggtacaa tgtactcgct ccacaaaaat ttaagaagat
     2761 tgagttactg aagtaatcag gtgttcccat tgccataaac catggaatgc aaatgagaag
     2821 tccattgctt aagttgagcc attgttgaca tgtaaccgtg tcctcaaaag aaccgttagc
     2881 aataatattt tgggaatgaa cgctattaaa ttgtaagata agtataatta ctattacaaa
     2941 tatttttctc atttcgaaag tagttttatt tttaaaataa tctgaggtta gtgacagact
     3001 attttataac aactacttta aacgctttgt taatgtgat
//
LOCUS       DAOMAA010000119         3038 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_25596_length_3038_cov_2.938652, whole genome shotgun sequence.
ACCESSION   DAOMAA010000119 DAOMAA010000000
VERSION     DAOMAA010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25596_length_3038_cov_2.938652"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="PLO59_02410"
     CDS             complement(<1..198)
                     /locus_tag="PLO59_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019946349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HQU99979.1"
                     /translation="MQTKNQKAERAVLVAVMRKADDVAQAEAYLNELTFLAETAGAQV
                     VYQFLQKLDAPDQRTYVGKGKL"
     gene            complement(223..828)
                     /locus_tag="PLO59_02415"
     CDS             complement(223..828)
                     /locus_tag="PLO59_02415"
                     /inference="COORDINATES: protein motif:HMM:NF016161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HQU99980.1"
                     /translation="MYTGIMHTHTLVVSLFLLIYLVKTGLLFFNKTAVLQTFSAKIKV
                     PEMIISTLFLLTGVYLAINTGNGGNWLWAKLVAVFVSIPLAVIAFKKQNKNLALLSLM
                     ALMYAYGVSETKSAFFKPEEKPGTASYNGREVYETYCISCHGADGKLMLSGAKDLTLS
                     PLTLDQRIALINSGKNAMPAYAKTLSAAQIQAVAVYTQTIK"
     gene            988..2019
                     /gene="ruvB"
                     /locus_tag="PLO59_02420"
     CDS             988..2019
                     /gene="ruvB"
                     /locus_tag="PLO59_02420"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017259380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HQU99981.1"
                     /translation="MNPNLNPQPEKLTTTERDIEKALRPLDFGDFTGQEKVVENLKIF
                     VGAASKRNEALDHVLLHGPPGLGKTTLANIIANELGVKIKTTSGPVLDKPGDLAGLLT
                     NLQPNDVLFIDEIHRLSPVVEEYLYAAMEDFKIDIMIESGPNARSVQIKLHPFTLIGA
                     TTRSGLLTAPLRARFGITSRLAYYDAKLLQLIVLRSAELLHAPINDEAAFEIARRSRG
                     TPRIANALLRRVRDFAQMKNNGKIDMHIAQFALNALHVDLHGLDEMDNRIITAIIEKF
                     KGGPVGITTIATAVSEEAGTIEEVYEPFLIQEGYLMRTPRGREATDKAYKHLGKNRPQ
                     GLGNLFDKE"
     gene            2281..2703
                     /locus_tag="PLO59_02425"
     CDS             2281..2703
                     /locus_tag="PLO59_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99982.1"
                     /translation="MNVDVGKLIENVSQIKLKGGIFSKASTVLMVISVCIFGIAALTG
                     NLYVSCTAIGLIFILAFVILWRLINFANKNPQAAILEGAEFLVHEQIQLAAKGITSIP
                     QSVSTLIEETPIKIDNESQRLANQPDENNKTGKEENNG"
BASE COUNT         1029 a          561 c          584 g          864 t
ORIGIN      
        1 aagcttgcct ttgcctacat aggtgcgctg atcgggtgca tcgagctttt gcaaaaactg
       61 atacactacc tgggcacctg cggtttcggc caaaaaagtt aattcattta aataagcttc
      121 ggcttgagcc acatcgtcag ctttgcgcat tacggctacg agtacagcac gctcggcttt
      181 ttggttttta gtttgcaaca gttagttatg gtttaaaaaa cattacttaa ttgtttgtgt
      241 ataaactgca actgcctgta tttgtgctgc acttaatgtt tttgcataag ccggcatggc
      301 atttttaccg ctattaatta aagctatgcg ttggtcaagt gttagaggcg ataaggttaa
      361 atcttttgcg ccactcagca tcaatttgcc atcggcacca tggcaactta tgcaataggt
      421 ttcgtaaacc tcacgaccgt tataagaggc agtacccggt ttttcttcgg gcttaaaaaa
      481 tgcacttttg gtttcgctaa cgccataagc gtacattaaa gccattaaac ttaataacgc
      541 taaattttta ttttgctttt taaaagcgat aactgccagt ggtatagaaa caaaaacggc
      601 aacaagtttt gcccataacc aattaccacc attgccggtg tttatagcca aataaacacc
      661 cgttagcaga aacaaagtgc ttataatcat ttcgggcact tttatttttg ccgaaaaagt
      721 ttgcagcact gctgttttgt taaaaaacaa aagacctgtt ttaactaaat aaataagtaa
      781 aaatagcgat accactaaag tgtgtgtatg cataatgcct gtgtacataa ctgactttgt
      841 ttaataatta acggctgcaa attagattaa taattgaagt gctgcccaat tttaaaacac
      901 ataaaattag atacactgcc acaggcatta aactcaattg tttatttaat tttgcaataa
      961 taaatgcgtt ttaattatta attgatgctg aacccaaatc ttaaccccca acccgaaaag
     1021 cttaccacca ctgaaagaga tattgaaaaa gctttgcgcc ctttagactt tggcgatttt
     1081 acaggacaag aaaaggtggt tgaaaactta aaaatatttg taggcgcagc cagcaaacgt
     1141 aacgaagctc ttgaccatgt tttattacat ggcccacccg gcttaggcaa aaccaccttg
     1201 gctaatatta tagcaaacga actgggagta aaaataaaaa ctacatcggg gccggtgttg
     1261 gataagccgg gcgacttagc cggtttactt accaatttac aacctaatga tgtactgttt
     1321 attgatgaaa tacaccgatt aagcccggtt gtagaagaat acttatatgc agccatggag
     1381 gattttaaaa ttgacatcat gattgaaagt gggcctaatg cacggtcggt tcagattaaa
     1441 ctacacccct ttacattgat aggcgcaacc acacgcagtg gattacttac tgcgccatta
     1501 cgtgcacgtt ttggtattac cagcagactt gcttattatg atgcaaaact attgcaactt
     1561 attgttttaa gatcggcaga gcttttacat gcacctataa atgatgaagc cgcatttgaa
     1621 attgccagac gcagccgtgg tactccacgc atagccaatg ctttactaag gcgtgtacgc
     1681 gattttgcgc aaatgaaaaa taatgggaaa attgacatgc atattgcaca atttgcatta
     1741 aacgcattgc atgttgactt gcatggcctt gatgaaatgg ataatagaat aattactgct
     1801 attatcgaaa aatttaaagg aggcccggta ggtattacta ctattgctac agctgtaagc
     1861 gaagaagcag gaaccattga agaagtgtac gagccctttt taatacaaga aggctacctg
     1921 atgcgcacac cacgcgggcg cgaagcaaca gacaaagctt acaaacactt aggcaaaaac
     1981 cggccccaag gtttaggtaa tttatttgat aaggaataaa cctagcaagt atcgtttgca
     2041 taaaagcatt ttaatatacc gttagtgcat aagatgcagc gttaaatact ttatgaatca
     2101 aattaaaaac ctttaactca tagaaaacga aatttgctta ggtaataaaa attaactgga
     2161 accaatcaaa ttgtaaaaca tacgataaac atacctatat tgagcggata cctgaaccta
     2221 aaaacggaat aggttcaggt ataaacaagt tatgcacaat tataaaacga cacagcaaat
     2281 atgaatgtag acgtaggaaa actaatagag aatgtttccc aaataaaact aaagggaggc
     2341 attttttcaa aagcaagcac ggtattaatg gtaatatctg tttgcatttt tggtatagcc
     2401 gccttgacag gtaacttata tgttagctgt acagctatag gactaatctt tatacttgcc
     2461 tttgtaatac tttggcgact tataaacttt gcaaataaaa atccacaagc agcaatacta
     2521 gaaggtgctg aatttttagt gcatgaacaa attcaattag cagctaaagg aattacatca
     2581 atccctcaat ctgtttcgac acttatcgaa gagacgccaa ttaaaattga caatgaatca
     2641 cagaggttag ctaatcagcc tgacgaaaat aacaaaacag gaaaggagga gaacaatggc
     2701 taacggaaaa ttttatcatg tatttataac acccaaaact ggtgttgacc ttgaagctat
     2761 aaaaactcaa atgaatctgg ctttagactg gttcaaatac gacaagaata attgggtttt
     2821 atattcaaac gcttctatgg acaaattaat gacacgcttt aagccattag ttgagccaga
     2881 agggcgattg tttatatgtg agttaaatac atcaaaccgg aatggttgga tgaacaagga
     2941 tttttgggaa tggttgaaaa agaacagaga caaataactg tgcataacag gcgtttggct
     3001 caatggcggg tgaagtggtt aattgaacat tctacctc
//
LOCUS       DAOMAA010000120         3037 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_25615_length_3037_cov_3.114017, whole genome shotgun sequence.
ACCESSION   DAOMAA010000120 DAOMAA010000000
VERSION     DAOMAA010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25615_length_3037_cov_3.114017"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /locus_tag="PLO59_02430"
     CDS             complement(<1..977)
                     /locus_tag="PLO59_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99983.1"
                     /translation="MASISLQFITDLIKGFFPYNFRVYMLDLGQLDTTYLYGHIKLFK
                     SNKTMKANHKTQAGKYYNLLAITTTILLLVTNAITIKAEAVYEFQNATSVVTTPANKV
                     YVTGYKNLFGTNTEIVTQVYTNKGTLINEVVNKNNNAELHNDQPYKVLADANNNIYVC
                     GHEFIDDLFGYEVVLIKYDENLNQLWKMVLYTSEHYADEARGIAIDGLGNICITGIQR
                     HFENFSKVFLYKINPAGEVVYYSNLPNDFDNKIEMVNNLIADKDGNVIICGTASNPLR
                     ATRFFTAKFDVAGALKWKRFNDCTTEKFNDEAFALSFDKWGNILVAGSS"
     gene            complement(996..2156)
                     /locus_tag="PLO59_02435"
     CDS             complement(996..2156)
                     /locus_tag="PLO59_02435"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HQU99984.1"
                     /translation="MKRIFYVVLVSFILGYNSQAQVSFIDNNWETAFAEARNTNKFVF
                     VDCYTDWCSWCKVMDKNTFSNTEVGSNMSEKFVALKLNMEKGYGINVAMKYGVSAFPS
                     YLVLNAEGYLIKKLMGYMEVQPFLQSLTSILENPTPINGYSNSIKTDYPLFYTYTFDG
                     NKETPRPAAEEVYKYLDTQTDLLNETNFNILKRYQINEKYAQVILNQYDNYKQMYGDE
                     ANDLIEKVIDVKLETAIANKSLEQLALVVDLPHKYQLANASETEVNLKAQYYLGINDC
                     DNYALTINQFVESQNEVMPATLNSYSWTVYEKCNSKKSIDLALVWIERALSKEEDYAS
                     MDTYAALLYKAKNYSKAKTIAEKAIKIGTADGEDVSETQKLLAKINAASIKK"
     gene            complement(2196..>3037)
                     /locus_tag="PLO59_02440"
     CDS             complement(2196..>3037)
                     /locus_tag="PLO59_02440"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HQU99985.1"
                     /translation="ISGIAWDHINVFPTFENYVSQFRIFADAIPPDGQLIYCINDDEV
                     NNVCMNTATDAIKTGYTIPPHQINNGVTYLADVTKSVETPLNFNIPLQIFGNHNLMNL
                     EGARLVCKAIGVEAAVFYKHIQIFKGAAKRLEKVWQQGSFILYKDFAHAPSKVLATTQ
                     AVKLQFYQYKIVACLELHTFSSLNDAFLSQYNQTLAAADMAFVYYNQQTIAHKKLNPI
                     STETVATAFGISAKQVFTQTDALISNLIALGTEKTILLMMTSGTFDRLNFESLAKQLT
                     ASN"
BASE COUNT          886 a          535 c          525 g         1091 t
ORIGIN      
        1 tcgctgctgc cagccaccaa aatgttgccc catttatcaa acgataatgc aaaagcttca
       61 tcgttaaact tttcagtagt gcaatcgtta aatcgtttcc attttaaagc acctgcaaca
      121 tcaaatttgg ctgtaaaaaa tcgtgttgct cttaaaggat tagaggctgt accacatata
      181 atcacattac catccttatc tgctatcagg ttgttaacca tttcaatttt attatcaaaa
      241 tcgtttggca gattgctgta gtaaactact tcgcctgctg gattaatctt atacaaaaaa
      301 actttgctaa agttttcgaa atggcgttgt atgcctgtta tgcaaatatt gcctaagcca
      361 tcaatagcaa taccacgggc ttcgtcagca taatgctccg atgtataaag caccattttc
      421 caaagttggt taaggttttc atcgtacttg ataagtacta cctcataacc aaataaatcg
      481 tcaataaatt catgaccaca tacataaata ttattgtttg catcggccaa tactttataa
      541 ggttggtcat tgtgcaattc ggcattgtta tttttattaa ctacttcgtt aatcaaggtg
      601 cccttattgg tgtaaacctg agttacaatt tcagtattgg tgccaaataa gtttttatag
      661 cctgttacat acactttgtt tgcaggcgtt gttacaacag atgttgcatt ttgaaactca
      721 taaacagctt ctgcttttat ggttattgcg tttgttacca aaagcaatat ggttgttgta
      781 atggcaagta agttatagta tttgccggct tgtgttttat ggtttgcttt catggttttg
      841 ttagatttga atagtttaat atgaccgtac aaatatgtcg tatcaagttg ccccaaatca
      901 agcatgtaaa ccctgaaatt ataagggaaa aaccctttaa ttaaatcagt tataaattgc
      961 aaactgatgc ttgccaaaaa tggttttcaa tctatttact ttttaatgga tgctgcattt
     1021 attttggcca atagtttttg ggtttcgcta acgtcctcgc catcggcagt tcctatctta
     1081 attgcctttt cggcaatggt ttttgccttg ctgtagtttt tagctttata caacaaagcc
     1141 gcataggtat ccatactggc atagtcttct tcttttgata aggcacgctc aatccataca
     1201 agggctaaat cgatagattt tttactgtta catttttcgt aaacggtcca gctataacta
     1261 tttagtgttg caggcattac ttcattttgc gattcgacaa attgattgat tgtaagtgca
     1321 tagttatcgc aatcgttaat acctaagtag tattgagctt taagattcac ttctgtttcg
     1381 gatgcattgg ccaactgata tttgtgtggc aaatcaacta ccagtgctaa ttgctccaag
     1441 ctcttattgg caatagcagt ttcgagtttt acatctatta ctttttcaat taaatcattg
     1501 gcttcgtcac catacatttg tttgtagtta tcatattgat ttaaaataac ctgtgcatat
     1561 ttttcattaa tctgataacg ctttaaaatg ttgaagtttg tttcgtttaa taaatcggtt
     1621 tgggtatcta aatatttgta aacctcttcg gctgcgggcc ggggtgtttc tttgtttcca
     1681 tcaaacgtgt aggtataaaa caaagggtaa tcggttttga tggaattgct gtaaccattt
     1741 atgggtgttg ggttttcgag tatggatgtt agtgattgca aaaagggctg cacctccatg
     1801 tagcccatta atttttttat caaatatcct tcagcgttta aaaccaaata tgatggaaaa
     1861 gcagaaacac cgtatttcat tgccacatta ataccatagc ctttttccat atttaattta
     1921 agtgccacaa atttttcgct catgttgcta cctacttcag tgttactaaa agtattttta
     1981 tccattactt tacaccaact gcaccaatcc gtgtaacaat caacaaatac aaacttgttt
     2041 gtattacgtg cttcggcaaa tgccgtttcc caattgttat ctataaaaga tacttgtgcc
     2101 tgactgttgt atcctaaaat gaatgatact agtactacat aaaaaatacg tttcataatt
     2161 ttttttgaat ttattgtttt gtaaggtgta tggttttaat tacttgctgt taattgtttt
     2221 gccaagcttt caaaattgag gcgatcaaat gtacccgaag tcatcattaa taaaattgtt
     2281 ttttctgtac caagtgcaat caagttcgaa attagagcat ccgtttgtgt aaatacctgt
     2341 ttggccgaaa taccaaaggc tgttgcaacg gtttctgttg atatgggatt taatttttta
     2401 tgtgcaatag tttgttggtt gtaataaaca aaagccatat cggctgctgc cagtgtttga
     2461 ttatactgag ataaaaatgc atcgtttaag ctactgaatg tatgcaattc taagcaagca
     2521 actattttgt attgataaaa ttgcagttta acagcttgtg tagttgccaa tacttttgaa
     2581 ggtgcatggg caaagtcttt atacagaata aaactgcctt gttgccatac tttttcaagc
     2641 cgttttgctg cacctttaaa aatttgaata tgcttgtaaa aaacggcagc ttctacacca
     2701 atggctttgc aaaccaatcg tgcgccttct aaattcatca ggttatgatt gccaaatatt
     2761 tgcagcggaa tattaaagtt taaaggtgtt tctacacttt tggttacatc agccaaataa
     2821 gtaactccat tattaatttg atgtggtggt atggtatagc cggttttaat ggcatcggtt
     2881 gcagtattca tacaaacatt atttacttca tcgtcattta tacaatatat aagttgccca
     2941 tcaggcggta tggcatcagc aaaaatgcga aattgcgaaa cgtaattttc aaaagttgga
     3001 aacacattta tatgatccca tgcaatgcca ctaatta
//
LOCUS       DAOMAA010000121         3037 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_25616_length_3037_cov_3.109658, whole genome shotgun sequence.
ACCESSION   DAOMAA010000121 DAOMAA010000000
VERSION     DAOMAA010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25616_length_3037_cov_3.109658"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2543)
                     /locus_tag="PLO59_02445"
     CDS             complement(<1..2543)
                     /locus_tag="PLO59_02445"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HQU99986.1"
                     /translation="MQQYQRINNILGWLVFAIASFVYLSTIEPTGSFWDCGEFIATAN
                     KLQVGHPPGAPMFLMMARVCIIFAGDNLQLIPIMVNSLSAIMSAFCILFLFWTITAFA
                     KKSVVKNSTNITNDQLITIMGSGFIGALAYTFTDSFWFSAVEGEVYASSGAFTACVFW
                     AIMKWDAVADEKHAMRYIILIAFLMGLSIGVHLLNLLAIPAVALVYYFRKYSFSWMGV
                     VKTLAIGVAILATIQFGIIQYYLKMGSMIDLFFVNSLGMPFWSGIILFNIVVIAAVAY
                     GIYYTQTKNIVLWNTVLLCFAFILIGYFSYAQVVIRSGANPPLDENDPENVFGLLSYL
                     NREQYGDFPLGYGQYFTATLEQQKPGATIYAQTKDKYIPVGNKPIAVYDKENCGVFPR
                     MWSPQDNHINAYKTWSGTPGDRRPTWGENMAFFWKYQINHMYMRYFMWNFVGRQNDVQ
                     GHGDILRGNWISGVPALDAMRLGPQENLPKSMTENKGRNKFYFLPLILGLLGMVYHFY
                     SDRKDFMIVGLLFFMTGFAILLYLNQTPYQPRERDYAYAGSFYAFAIWIGLGVASIAE
                     MLKGKINARMAAIASIVVSLCVPYILAAEGWNDHDRSHRYTSRDFAADYLNSCAPNAI
                     IFTNGDNDTFPLWYVQEVENVRTDIRIVNLSLLNTDWYINQMRRQAYKSAPVPFTLPS
                     EKYQQGTRDYIPFYDKKVPGYTDLNEVFNFITSDDEQFKLQTRGGGAINYMPTKKLRL
                     QVDKQAALKSGTVALKDTAIMVDVMEWDYGKDYVSKADLMMLDLIVHNNWQRPIYYAV
                     TIGSDGYIGLENYFQAEGLAYRLVPIKAIKNDGQPGRVGTDQMYDNVMN"
BASE COUNT          920 a          616 c          602 g          899 t
ORIGIN      
        1 ttattcatta cattatcata catctgatcg gtacctacac ggccgggttg cccatcattt
       61 ttaattgcct ttatgggcac taaacggtag gctaaacctt cggcctggaa ataattttct
      121 aaaccaatat agccatcgct gccaattgta actgcgtaat agatggggcg ttgccagtta
      181 ttgtgtacaa ttaaatcaag catcattaaa tcggctttcg atacatagtc tttgccataa
      241 tcccactcca ttacatcaac cataatggct gtatctttta atgcaacggt gcccgattta
      301 agtgctgctt gtttatcaac ctgcaacctg agttttttgg taggcatata gtttatagca
      361 ccgccaccac gggtttgcag tttaaactgc tcgtcatcgc tggtaataaa attaaacact
      421 tcgttaaggt cggtatagcc gggcactttt ttatcgtaga aaggtatgta atcgcgtgta
      481 ccttgctgat atttttcgga aggcaaagta aacggcaccg gtgccgattt gtatgcctgt
      541 ctgcgcattt gattgatata ccagtcggtg tttaataagc tcaggtttac gattcgaata
      601 tcggtgcgta cgttttcaac ttcttgtaca taccataatg ggaacgtatc gttatcgcca
      661 ttggtaaaaa taattgcatt gggtgcacaa gagtttaagt aatcggcagc aaaatcgcgc
      721 gaggtgtatc ggtgcgatcg gtcgtggtcg ttccatcctt cggcagctaa tatatagggt
      781 acacaaagcg aaactacaat ggaggcaatg gctgccatac gtgcattaat tttgccttta
      841 agcatttcgg caatagatgc tacgcctaag ccaatccata tagcaaatgc ataaaacgaa
      901 cccgcataag cataatcacg ttcgcggggc tgataggggg tttgattgag gtaaagtaaa
      961 atggcaaagc ctgtcataaa aaacagcaat cccacaatca taaagtcttt tctatcgcta
     1021 taaaaatgat ataccatacc taacagacct aatattaaag gcaggaaata aaatttgtta
     1081 cggcctttat tttcagtcat acttttaggc aagttttcct gagggcctaa gcgcattgca
     1141 tctaaagcgg gcacaccgct aatccaatta ccgcgtaaaa tatcgccatg gccttgcaca
     1201 tcgttttggc ggccaacaaa attccacata aagtagcgca tatacatgtg gttaatttga
     1261 tatttccaga aaaaggccat gttttcgccc caggtgggtc gtctgtcgcc cggtgtgccg
     1321 ctccaggttt tgtaggcatt gatatggtta tcctgcgggc tccacatgcg tggaaaaaca
     1381 ccacaatttt ctttgtcata aacagctata ggcttattgc ctaccgggat atacttatct
     1441 tttgtttgcg cgtaaatagt agcaccgggt ttttgttgtt ctaatgttgc cgtaaagtat
     1501 tggccatagc ctaaaggaaa atcgccatac tgctcgcggt ttaaatagct taaaagtccg
     1561 aatacatttt cggggtcgtt ttcgtctaat ggtgggtttg cgcccgacct tataactact
     1621 tgcgcataac taaagtatcc tatcaatata aatgcgaagc ataataaaac cgtattccac
     1681 aatactatat ttttggtttg tgtgtagtaa ataccgtaag caactgctgc aattacaact
     1741 atgttaaaca aaataatgcc cgaccaaaat ggcataccca ggctgtttac aaaaaacaaa
     1801 tcaatcatac tacccatttt aaggtaatat tgaataatac caaattgaat ggttgctaaa
     1861 atagccacac caatagccag tgtttttaca acacccatcc agctaaagct gtatttgcgg
     1921 aaataataaa ccaatgcaac ggcaggtatt gccaacaggt ttagtaagtg gacgccaatg
     1981 gataagccca ttaaaaaggc tatcagaatt atgtagcgca tggcatgttt ttcatcggct
     2041 acggcatccc atttcataat ggcccaaaat acgcaggctg taaatgcacc tgaactggca
     2101 tatacttcgc cctctacggc actaaaccaa aatgagtcgg taaaggtgta tgccaaagca
     2161 ccaataaaac cactgcccat tatggttata agctgatcgt tggtaatgtt tgtactgttt
     2221 tttacaaccg attttttagc aaatgccgtt atggtccaaa ataaaaacaa aatacaaaaa
     2281 gcactcatta tggccgatag tgaattaacc ataatgggta taagctgtaa attgtcgcct
     2341 gcaaaaataa tacatacgcg ggccatcatt aaaaacatgg gcgctccggg tgggtggcca
     2401 acttgcagtt tattggccgt tgctataaac tcaccacaat cccaaaaact tccggtaggt
     2461 tcgatggttg ataggtaaac aaatgaagct atggcaaaaa caagccagcc caaaatgtta
     2521 ttaatacgtt gatattgttg cataatgcgt ttttataaag cgtgcgaata tataagttta
     2581 accctaaccg caggatatgc tatttatttt ggtactggtg ttaaaaacgg tgtaaactaa
     2641 accaacgatt gatgcccttt tgcttgtcat tacgaaacga ttacatttcg attagtgccc
     2701 aataatgctg ctacctgtta tccgttaaca acttgttttg tttcctgttg tatttaattg
     2761 taaccgttgc gcatttagta agtttagtca ggttacataa caacccatta aatgttgtaa
     2821 tataaaaagc gccttaaagt taatggtgtg tgatttgggc aaaaaaacag ccgccttaaa
     2881 agtttaaggc ggctgttttt tatactttaa aaactgaata aactattgta agatgatacg
     2941 gtggcgggca atacctgtat tgttttgtac ctgtaaataa tatacaccac gttgtaaagt
     3001 agctaccggt atgttgtaat gcataactgc accactt
//
LOCUS       DAOMAA010000122         3036 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_25635_length_3036_cov_3.622610, whole genome shotgun sequence.
ACCESSION   DAOMAA010000122 DAOMAA010000000
VERSION     DAOMAA010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25635_length_3036_cov_3.622610"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="PLO59_02450"
     CDS             <1..467
                     /locus_tag="PLO59_02450"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQU99987.1"
                     /translation="VITDIRIIVRNLAAKYISEKGIYLQLHEFKKQIEKGSPIKIDLE
                     IESFKEIQDPDFAINLYRIIQELINNTIKHAACTRIDIIAHKQAGLLRVLYKDNGKGF
                     DEMRVNKGLGLNNVLTRVRLYKGVCEVVSKPGQGTCYSIDFQLKHTTLTITT"
     gene            479..1138
                     /locus_tag="PLO59_02455"
     CDS             479..1138
                     /locus_tag="PLO59_02455"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQU99988.1"
                     /translation="MENLKVLIADDHQMFRKGLELSIKTINSVGKILQAENGLFVMDI
                     LSKEEVDIIFMDIKMPHQNGIETTKLVVQKYPRVKVVALSMYDDKDNILEMFKAGVNG
                     YLLKNTNKAEIEMAINEVMMGGKYYSKEVSDVLLQRIIHAETSTYNSDTSNQLTDREK
                     EVLKYVCAQMSTKEIADVMCLSDKTIEGHRIRLIQKTNSKNLAGLVLYAVEHGLVDKH
                     R"
     gene            complement(1270..1881)
                     /locus_tag="PLO59_02460"
     CDS             complement(1270..1881)
                     /locus_tag="PLO59_02460"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HQU99989.1"
                     /translation="MKKTLFLLAIASFALLKSQAQFVLNPQAGITAQTAIKALDNEEF
                     KARVGYTLGTDARFGSRFYFQPGLYFSAVTSEYQYEDGVDTTDYDYKTDFQRQTLKLN
                     AKAGYNIVHKDGFKLRVNAGPSFDVLLNAKSDNDAIDDDDFKKGFVNLDAGVGLDIWF
                     VSVDLSASYGLTKAFDNTTTENNTRFLTGFLTVGIVLGNGLKD"
     gene            complement(1895..2410)
                     /locus_tag="PLO59_02465"
     CDS             complement(1895..2410)
                     /locus_tag="PLO59_02465"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HQU99990.1"
                     /translation="MKLTILISSLCILSTNVHAQLLLKSSDGKNDMVAIPISKGYIND
                     REKIFSETEKNQIRDLMIKFRFDSNTISILTVAEIDPFENIQALAVAYANGWELGWGN
                     KNGVLFVFSKSKQKSTVQIGEALAKKISTDQLNYLDRLMIQQFVQNNYAQGVINALSE
                     LNQILIQTNKH"
     gene            complement(2407..>3036)
                     /locus_tag="PLO59_02470"
     CDS             complement(2407..>3036)
                     /locus_tag="PLO59_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008243990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQU99991.1"
                     /translation="GRTGSGKSTVANLMVRLYDVSEGEILIDGINIKDIPIHIVRAAI
                     GYVPQEVFLFSDTIKNNIAFGYTNASESEITHAAIAADVYQNIIGFENKFETLLGERG
                     INLSGGQKQRISIARALVKNAKILIFDDVLSAVDTKTESKILNNLKPILAHQTCFIIS
                     HRITSIQHANQIIVLENGVVVETGNHQTLINNNGYYARQYQLQMEEEAA"
BASE COUNT          996 a          514 c          528 g          998 t
ORIGIN      
        1 acgtaattac ggatattaga attattgtgc gcaaccttgc agcaaagtat attagtgaaa
       61 aaggtatata ccttcaatta catgaattta aaaaacaaat tgaaaaagga agccctataa
      121 aaattgattt agaaattgaa agttttaagg aaatacaaga tcccgacttt gcaataaatt
      181 tgtatcgcat tattcaggaa cttataaata atacaattaa acatgcagca tgtacgcgta
      241 ttgatattat agcacataag caagccggtt tgttaagagt attatacaaa gataacggta
      301 aagggtttga cgaaatgcgc gtaaataagg gattaggttt aaacaatgtg ctaacccggg
      361 tcagattata taaaggggtt tgtgaagtgg tttcaaaacc ggggcaaggc acttgctatt
      421 caatagattt tcaattaaaa cacactacac ttactattac cacctaataa accaaaccat
      481 ggaaaatctt aaagtactta tcgctgacga ccaccaaatg ttcagaaaag gtttagagct
      541 atccatcaaa acaataaata gcgttggcaa aatattgcaa gccgaaaacg gtttgtttgt
      601 gatggatatt ttatcgaaag aagaagtaga tattattttt atggatatta aaatgccgca
      661 ccaaaacggc attgaaacca caaagttggt tgtacaaaaa tatccgcgag taaaagttgt
      721 tgctttaagt atgtatgatg ataaagataa tatacttgaa atgtttaaag caggtgtaaa
      781 tggctactta cttaaaaaca cgaataaagc cgaaatagaa atggccatta acgaagttat
      841 gatgggcggc aagtattata gcaaagaagt atcggacgtg cttttacaac gcattataca
      901 tgccgaaacg agcacttaca acagcgatac gagcaaccaa cttaccgacc gtgaaaaaga
      961 ggtgcttaaa tatgtatgtg ctcaaatgag tacaaaagaa attgccgatg taatgtgcct
     1021 gagtgataaa accatagaag gccatcggat tagattgatt caaaaaacaa attcgaaaaa
     1081 tttggccggt ttggttttat atgctgttga gcatggcctg gttgataagc ataggtaaat
     1141 gttaatcgca attataaata ccagatctat ctgttttgag caaaagcaaa accactaaaa
     1201 tcaggcaaac cgtatagtaa aaaaaaagcc gtataatttc ttattcggct tttttttgtt
     1261 gcatataaat taatccttta aaccattacc taatacaata cccactgtta aaaatccggt
     1321 taaaaaccgt gtgttgtttt cagtagttgt gttgtcaaaa gcttttgtta gtccgtacga
     1381 agcgcttaag tcaaccgata caaaccaaat atccaaaccc acacctgcat ctaaatttac
     1441 aaagcccttt ttaaaatcgt catcatcaat ggcatcgtta tccgatttgg cgttaagtaa
     1501 aacatcaaag ctggggcctg catttacccg taatttaaat ccatccttat gtacaatatt
     1561 ataaccggct tttgcattaa gttttaaagt ttggcgctga aaatcagttt tgtaatcata
     1621 gtctgtggtg tcaactccgt cttcatattg gtattcagaa gttactgcag aaaaatatag
     1681 cccgggttga aaataaaaac ggctgccaaa gcgtgcgtcc gtacctaatg tataaccaac
     1741 gcgggcttta aactcttcgt tatctaaagc tttaatggct gtttgtgctg taataccggc
     1801 ttgtgggttt aatacaaatt gtgcttgtga ttttaataaa gcaaaacttg ctattgctaa
     1861 aaggaataat gtttttttca ttttaattta tttattagtg tttgttagtt tgtattaaaa
     1921 tttggttcaa ttcgcttaaa gcatttatta caccttgtgc ataattgttt tgtacaaatt
     1981 gttgaatcat taatcggtct aaataattta attggtctgt actgattttt tttgcaagtg
     2041 cttcgcctat ttgtaccgtt gacttttgtt tcgatttgct aaagacaaat aatacgccat
     2101 ttttattgcc ccaaccaagt tcccatccat tggcataagc tacagcaagt gcttgtatat
     2161 tttcaaacgg gtcaatttcg gctaccgtta atatgcttat ggtattcgaa tcgaaacgaa
     2221 attttatcat taaatcgcgt atctgatttt tttctgtttc actaaatatt ttttctctgt
     2281 catttatata acctttgctt ataggtatag caaccatatc atttttgcca tcactacttt
     2341 ttagcagtaa ttgcgcatgt acatttgtgc taagaataca taaactggaa attaatatag
     2401 ttagtttcat gcggcttctt cttccatttg taattgatat tgacgtgcgt aatatccgtt
     2461 attattaatt aaggtttgat gattgcccgt ttctacaaca actccatttt ctaacactat
     2521 aatttgattt gcatgttgta tggaagtaat ccggtgtgag attatgaagc aagtttgatg
     2581 agccaaaatt ggctttaagt tgtttaaaat ttttgattcg gttttcgtat ccactgcact
     2641 taaaacatca tcaaaaatta aaatttttgc atttttaacc agcgctctgg ctattgaaat
     2701 gcgctgcttt tgtccacccg aaaggtttat cccacgttcg cctaacaggg tttcaaattt
     2761 gttttcgaaa cctataatat tctgatatac atcggctgca atagcagcat gggtaatctc
     2821 cgattctgat gcatttgtat aaccaaaagc gatattgttt tttatggtat cgctgaaaag
     2881 aaacacttct tgtggtacgt aaccaatagc tgcacgaact atatgtattg gtatgtcttt
     2941 tatattaata ccatcaatta atatttcgcc ttccgataca tcatacaaac gaaccattaa
     3001 atttgcaacc gttgatttgc cactgcctgt gcgacc
//
LOCUS       DAOMAA010000123         3030 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_25736_length_3030_cov_2.930420, whole genome shotgun sequence.
ACCESSION   DAOMAA010000123 DAOMAA010000000
VERSION     DAOMAA010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25736_length_3030_cov_2.930420"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1522)
                     /locus_tag="PLO59_02475"
     CDS             complement(<1..1522)
                     /locus_tag="PLO59_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009411031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQU99992.1"
                     /translation="MLDNIIAFSIKNKFIIGMMTVALITWGLWSAQQIPIDAVPDITN
                     NQVQIITSCPTLAGQEVEQLITYPIEQSIANLPQLEKLRSVSRFGLSVITVVFNDAVD
                     IYYARQLINEKLKEAEEHIPPGSGKPELAPVSTGLGEVYQYIIHPKKGSENKYTAMDL
                     RTMQDWIVARQLYGTPGIAEVNSFGGELKQYEVAVNPNRLLAMQVTIPEIFNALEKNN
                     ENTGGAYIDKKPNAYFIRGVGLIGSFDDIKNIAVKTNANGIPILIKDVAEVRFGSAVR
                     YGAITYNGEKDAVGGIVMMLKGYNSAELVNRVKEKMKTIQASLPPDVMIEPFLDRTDL
                     VQRAISTVEKNLIEGALIVIFVLVLFLGNFRAGLIVASAIPLSMLFALGLMNVFDVSA
                     NLMSLGAIDFGLIVDGAVIIVEATLHHLGLRKSINKLTQSEMDDEVFLSASKIRSSAA
                     FGEIIILIVYIPILSLIGIEGKMFRPMAMTVGFAIFGALILSLTYIPMMCALFLPKT"
     gene            complement(2354..>3030)
                     /locus_tag="PLO59_02480"
     CDS             complement(2354..>3030)
                     /locus_tag="PLO59_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99993.1"
                     /translation="IMKKQILCVIALIATTTLKAQTISISEQIANLSVGAQNALSTTI
                     YQSKKDDVINQWKSYLKNFKHEKIKMNGNELFGDNIVLKDWGNNPIDIYTIFEENKSD
                     TSVKVIAAFNLGGAYLSSTNDIAKYALAEKMLKDFALKSTSEAINEKLKLAEKLLDKL
                     HEGQRDLEKENKNFVTDIENYKSKISKAQDDIMTNENNQIKKKAEIEAQQSLVEQIKK
                     ELGRIN"
BASE COUNT          925 a          554 c          506 g         1045 t
ORIGIN      
        1 ttgttttagg taaaaacaaa gcacacatca taggaatata agttagcgat aaaattagag
       61 ctccaaatat ggcaaaacca accgtcattg ccatcggacg aaacatttta ccttcaatac
      121 ctattaagga aagaataggt atgtaaacta ttagaataat tatttcacca aatgctgcac
      181 tactccttat tttagaagca gataaaaaca cttcatcgtc catttcggat tgggttagtt
      241 tattaattga ttttcgcaaa cctaaatggt gcaaggtggc ttctacaatt atgactgcac
      301 catctacaat tagaccgaag tcaattgcac ctaaactcat taaattggca cttacatcaa
      361 atacattcat taagcccaaa gcaaatagca ttgatagtgg aatggcagaa gcaacaatta
      421 aacctgctct aaaattgcct aagaaaagaa ctaaaacaaa aattacaatt aaagcacctt
      481 ctattaagtt tttttctacg gtgcttatcg cgcgctgtac caaatcagtt cggtctaaaa
      541 atggttcgat cattacatca ggtggcaacg atgcttgtat ggttttcatt ttttccttaa
      601 ctcgatttac cagctcggca ctgttatatc ctttcaacat catcacgatg cctcctacgg
      661 catctttctc accattatac gtgattgcac catatcttac cgcactgcca aagcgcacct
      721 cagcaacatc ttttataagt atagggatac cgttagcatt ggttttaact gcaatatttt
      781 ttatgtcatc aaatgagcca attaatccta cacctcgtat aaaatacgca ttgggctttt
      841 tatcaatata ggcacctccg gtattttcat tattcttttc caatgcatta aaaatttccg
      901 gtattgttac ctgcatggcc agtaagcgat ttgggtttac agctacttcg tattgtttta
      961 actcgccacc aaatgaatta acttcggcaa tgcccggtgt gccgtaaagt tggcgtgcta
     1021 caatccaatc ttgcatagta cgtaaatcca ttgcagtgta tttattttcg ctgccttttt
     1081 ttggatgtat gatgtattga tacacttcac ccaaaccggt acttaccggt gcaagttcgg
     1141 gtttgccgct tcctggtggg atgtgttctt cggcttcttt tagtttttca tttattagct
     1201 gacgggcata ataaatatct acggcatcgt taaacacaac ggttattacc gacaaaccaa
     1261 aacgggaaac acttcgcaac ttttctaatt gtggtaagtt ggcaatgctt tgttcgatgg
     1321 ggtaggttat caattgctct acttcctggc ctgcaagtgt tgggcaagaa gtaattattt
     1381 gtacctgatt attggtaatg tcgggtacgg catcaattgg tatttgttgt gcactccaca
     1441 aaccccatgt tataagtgca acagtcatca taccgatgat gaatttattt tttatgctaa
     1501 aagcaatgat gttgtctaac attgatttta atgttacttt tttttaaaat atagtttaag
     1561 tatgcttagg gcactatatt ttataggcct aaacaaatag ttggtatgct gtaaaccgca
     1621 accggttacg atacatttag aaaagtgaaa ctttaagcta atttgggtgg ttgccaaata
     1681 ggatcgttaa tcgcactata aaaaaccggt tttaatattg aaaaaattcc tttgtggctt
     1741 ggcactaaga atacaagatt tgtattagca gtaatgatta cagttgtagc acaacacgta
     1801 caataacaca aagggctgca ttggtcagat tcctgatggt ggtgttcttg cgcagttgca
     1861 aaattatcag gtgtaatgtg cacacttgca tggcaattat cactgcaagg catacaaagc
     1921 aatgctataa agtataaagc caaaaaaccc gatatgtaat tttttaaaag cacgaagcaa
     1981 atataacgtt ttcggaacca aattataatt tgttactaaa gcctcttact attataatat
     2041 gcccactgca ataatatttt ttattgtaag caagtatttg ccggaagtta ttatggcaaa
     2101 acattttgtg ttaaaaagaa aaacgattga acattcacag catatacaaa ccaaaaaact
     2161 taaaactata aaaagtaaga aattattttt tgttgaaagt gccctgtttg taacacttac
     2221 cgtacatgta caggcacaat ggcaggttgg cgaataggtt ttagatgcag gcggatttat
     2281 aattgaaacc aatttatttt aaagcagata tttaaaaaat ccgatttgtt tactgcacgt
     2341 gtagcaaagc attttaattt atacggccaa gttctttttt aatttgttct actaacgatt
     2401 gttgtgcttc aatttcagct ttctttttta tttggttgtt ttcattggtc attatatcat
     2461 cctgtgcttt actaatttta gatttgtagt tttcaatatc agttacaaaa tttttatttt
     2521 ctttctccaa gtcgcgttgc ccttcatgca gtttatccaa aagcttttcg gccagtttta
     2581 atttttcatt tatggcttcg ctcgtagatt ttaatgcaaa atctttcagc attttttcgg
     2641 ccagtgcata tttagcaata tcgtttgttg agcttaaata ggctccacct aaattaaaag
     2701 ctgcaataac cttaaccgat gtatcgcttt tgttttcttc gaaaatggta taaatatcta
     2761 tgggattatt accccagtcc ttcaatacaa tattatcgcc aaacaactca ttgccattca
     2821 ttttaatttt ttcatgctta aaatttttaa ggtagctttt ccattgattg attacatcat
     2881 cttttttact ttgataaatg gttgttgata atgcattttg cgcaccaacc gataagttgg
     2941 caatttgctc gcttatacta atagtttgtg cctttaaggt agtagtcgca ataagtgcaa
     3001 taacacataa aatttgtttt ttcataatct
//
LOCUS       DAOMAA010000124         3026 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_25788_length_3026_cov_2.986873, whole genome shotgun sequence.
ACCESSION   DAOMAA010000124 DAOMAA010000000
VERSION     DAOMAA010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25788_length_3026_cov_2.986873"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..151
                     /locus_tag="PLO59_02485"
     CDS             <1..151
                     /locus_tag="PLO59_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016878912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HQU99994.1"
                     /translation="AMAGIKIVCAVGAPSNLAIQTADKFNITLIGFLRDNRFNIYTHT
                     TNVIA"
     gene            188..1843
                     /locus_tag="PLO59_02490"
     CDS             188..1843
                     /locus_tag="PLO59_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HQU99995.1"
                     /translation="MSKNLSNLAGRKGLQQTLFEQLGKAASKTGTPTPDEIEKLAEQF
                     VMGAANVYGTTTFYDFLRPENQGKKVYVCNGSACLTAGTQHQLKANLLQQFTADEIGE
                     MCCLGRCHQNSAFFYNQKNYSGNAITKFNRHQADQNATDNYYVGGSGKQLLIQNIENI
                     IDYYQILASALKQNPVTLLNELKQSGLRGRGGAGFPIAIKLESCSNEISDKKFIVCNA
                     DEGDPGAYSDRYLLEQQPHKVLLGMLLAGFMANARYGVLYIRAEYPEAIAIIKQQIEF
                     LHNAGLLGEQILGSDFSFSFKVIAAQGAYICGEETALLSSIEGQRPEVRVRPPYPVQK
                     GLFNKPTVVNNVETLACIPYIIQNGGHAFAAIGKGKSTGTKLVSLDSHFNRPGIYEID
                     MGTPLQQVIETLGMGFKSAIKAMHIGGPLGGLVPVHCIKDLTVDFESFTANGFLLGHA
                     SVVCIPQTFNMVDYLKHLFQFTAHESCGKCFPCRLGSTRGAELLEQAISHNNPIDAHL
                     FSDLLNTMEIGSLCALGGGLPLPVKNALQYFKDELAPYFKNQK"
     gene            1866..>3026
                     /locus_tag="PLO59_02495"
     CDS             1866..>3026
                     /locus_tag="PLO59_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HQU99996.1"
                     /translation="MQTTKTISQHPIAPTQVVQTTAFVNNQPYTIIPGETILSFLRRH
                     FGNDTVPTLCDAPNLEPFGSCRVCSVDVALTANGSTKAQASCHTPVMAGSYIYPDSDR
                     IQQLRKNIIELVLTDHPLDCLTCEVNNNCELQTVAARVGIREVRYPDGKNHLYKKHEK
                     DHSHPYMTSDFSKCINCFRCVRACDEVQGQFVLSMAGRGFDSHIVKGQDVSFNDSDCV
                     SCGACAQACPTSAISDVFESKSIVYDKKVRTVCTYCGVGCNLEVAVRADKIKSIQAPV
                     NAAANGGHTCLKGRYAFSFHNHGDRLQSPLIKRNGVFETATWDEAYEYIAERLTQIKN
                     QYGPDAIGGISSARCTNEENYIMQKFIRAVIGTNNIDSCARVCHSPTALGMQR"
BASE COUNT          961 a          586 c          611 g          868 t
ORIGIN      
        1 agccatggct ggtattaaaa tagtatgtgc agtgggtgcg ccatcaaact tagccataca
       61 aacagccgat aaatttaata ttaccttgat aggattttta cgtgataacc gctttaatat
      121 ttacacacat accaccaatg taattgcata gtatttagtt ttaaaatcca ttatcttagt
      181 agccatcatg agcaaaaact taagcaacct tgcaggccgt aaaggactac aacaaacctt
      241 gttcgaacaa ttaggtaaag ctgcatctaa aaccggtaca cctacacctg atgaaattga
      301 aaaattagcc gaacaatttg taatgggtgc tgccaatgta tatggtacca ccacatttta
      361 tgatttttta cggcccgaaa accaaggcaa aaaagtgtat gtatgcaatg gttcggcttg
      421 tttaactgcc ggtacccaac atcaactaaa agctaattta cttcaacaat ttactgctga
      481 cgaaattggt gaaatgtgtt gtttgggtag gtgccaccaa aacagtgcat tcttttataa
      541 ccaaaaaaac tattcaggta atgcaattac aaaatttaac aggcatcagg ctgatcaaaa
      601 tgcaaccgac aattattatg ttggtggaag cggcaaacaa cttcttattc aaaatattga
      661 aaatataatt gactactacc aaattttagc atcggcatta aagcaaaacc ctgtaacctt
      721 attaaacgaa ttaaaacaat cgggcttgcg tggcagaggt ggtgccggtt ttcctattgc
      781 cataaagctc gaaagttgta gtaatgaaat ttcggataaa aaatttattg tttgtaatgc
      841 cgatgaaggt gaccccggtg catatagcga ccggtattta ttggagcagc aaccgcataa
      901 agttttactg ggtatgttat tggccggttt tatggctaat gcacgatatg gcgttttata
      961 catccgtgcc gaataccccg aagctattgc tattattaaa caacaaattg agtttttaca
     1021 taatgccggt ttattaggcg aacaaatttt aggtagcgat tttagtttta gctttaaagt
     1081 aattgcagct cagggagctt atatatgtgg cgaagaaaca gcattactat cgagcattga
     1141 aggccaaagg cccgaagtaa gggtacgccc accctacccc gttcaaaaag ggttgtttaa
     1201 taagcctacc gttgtaaata atgttgaaac cttggcttgt attccataca taattcaaaa
     1261 tggcggacat gcttttgcag caattggcaa aggaaaatct accggtacta aattggtttc
     1321 attagatagt catttcaacc ggccgggcat ttacgaaatt gatatgggaa ctcccttaca
     1381 acaggtaatc gaaacattag gcatgggctt taagtcagct ataaaagcca tgcatattgg
     1441 tgggccattg ggtggtttag tacctgtaca ttgcatcaaa gatttaacgg ttgattttga
     1501 atcgtttact gcaaatggat ttttactagg tcatgcatcc gttgtttgta ttccacaaac
     1561 atttaatatg gttgattact taaaacattt atttcagttt acagcacatg aaagttgcgg
     1621 caaatgtttt ccatgtcgtt tgggctcaac ccgtggtgcc gaactgctcg agcaagctat
     1681 aagccataac aacccgattg atgcacattt attcagcgat ttacttaata caatggaaat
     1741 aggttcatta tgtgctttag gaggcggttt gccattgcct gtaaaaaacg ccttgcaata
     1801 ttttaaggat gaattggcgc catattttaa aaatcaaaaa taatactgca taaaaattta
     1861 tagctatgca aaccacaaaa acaatttcgc aacatcccat tgcaccaaca caagttgtgc
     1921 aaacaacagc ttttgtaaat aatcaacctt atacaataat acctggcgaa accatattaa
     1981 gttttttacg aaggcatttt ggtaatgata cagtacctac actgtgcgat gcacccaacc
     2041 tcgaaccatt tggatcgtgc cgcgtttgca gtgtggatgt agcacttaca gctaacggaa
     2101 gcacaaaagc acaggcatcg tgccataccc cggtaatggc tggcagctat atatatcccg
     2161 acagcgaccg catccaacaa ttacgtaaaa atattatcga actcgtatta accgaccacc
     2221 cactcgattg tttaacttgt gaggtaaaca acaattgcga attgcaaact gtagccgccc
     2281 gtgtgggcat tcgcgaagtt aggtatcccg atgggaaaaa ccacttgtat aaaaagcatg
     2341 aaaaagatca cagtcatcct tacatgacat ccgattttag taagtgtata aattgtttcc
     2401 gttgtgtacg tgcctgcgat gaagtgcaag gccagtttgt tttaagcatg gcaggccgtg
     2461 gtttcgatag tcatattgta aaaggacagg atgtaagttt taatgatagc gattgtgtaa
     2521 gctgtggcgc ctgtgcacag gcatgcccta cttcagctat tagcgatgtg ttcgaatcaa
     2581 aatctatcgt atatgataaa aaagtgcgca ccgtttgtac ctactgtggt gtaggatgta
     2641 atttggaagt ggctgtacgt gccgataaaa ttaaatccat acaagcaccg gtaaatgccg
     2701 ctgccaatgg tggccatact tgcttaaagg gccgatatgc tttttcattt cataatcatg
     2761 gcgatagact tcaatcgccc ctgataaaaa gaaatggtgt ttttgaaacc gcaacatggg
     2821 acgaagctta tgagtatata gcagaaaggt taacacaaat aaaaaatcaa tatgggcctg
     2881 atgccatagg cggtatttca tcggcacgct gcaccaatga agaaaattac attatgcaaa
     2941 aatttattcg tgccgtaatt ggcaccaata atattgatag ttgtgcccgt gtatgccaca
     3001 gtcccactgc tttaggcatg cagcgt
//
LOCUS       DAOMAA010000125         3021 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_25854_length_3021_cov_3.123399, whole genome shotgun sequence.
ACCESSION   DAOMAA010000125 DAOMAA010000000
VERSION     DAOMAA010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25854_length_3021_cov_3.123399"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3021)
                     /locus_tag="PLO59_02500"
     CDS             complement(<1..>3021)
                     /locus_tag="PLO59_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU99997.1"
                     /translation="ANYQWSNGVTTQNNIINASNTYTVTVTDANGCTGTASNTLIVNA
                     LPVPNITGTSTVCQGIAATLNANAGFSDYLWSNGTTTATINTTIAGSYTVTVTDINSC
                     TATVSQNVIVNANPAPVITGIMAICNGTNTTLNGGAGFSSYVWSNGSTTQSNLLTTAG
                     TYTVTVTNINGCTGTNSATLVVNANPTPVVTGLNAICAGANTSFDAGTGYSSYLWSNG
                     VTTQNNLINTANTYTVTVTNINGCTGSANNTLVVNALPTPNISGVTAVCQGFAATLNA
                     NAGYTNYVWSNGALTATINPTVSNTYTVTVTDGNGCTSSVAQAVVINALPTPSITGIA
                     AVCQGANANLNAGAGYSLYQWSDGSSTQVINPGIAANYTVTVTDANGCTGSANINVVV
                     SNNPTPSITGVNVICQNDPTTFDAGAGYSQYQWSNGNVTQNINPTTAGAYTVTVTDGN
                     GCTATASYSLTVNALPTPNISGVTAVCQGFAATLNTNAGYSNYVWSNGATTATINPTV
                     ANTYTVTVTDGNGCTNSVAQAVVINALPTPTITGQAVVCQGNNANIDAGAGYANYQWS
                     NGSSTQIINPNVAAIYTVTVTDANGCTGSTNINVVVNQNPTPAITGVNVICQNDPTTF
                     DAGAGYSQYQWSNGNVTQNINPTTAGAYTVTVTDGNGCTATASYSLTVNALPTPNISG
                     VTAVCQGFAATLNTNAGYTNYIWSNGATTASINPTVANTYTVTVTDGNGCTNSVAQVV
                     VINALPTPAITGITSICDGTTSSFDAGSGYSNYQWSGSNTTQILTTGVAGAYTVTVTD
                     NNGCSASTSIVLTVNQNPTPKIVGNDTICFGFVSLIDAGNGFVNYVWSTGLNAQIINI
                     NTAGNYTVTVTDANGCTGEDVFKLEVLTAEASISPNGPTSFCDGEQVTLQASMGLSYL
                     WSTGATTRDIVVKTSGNYTVQIVNLNGCTAVSTIQNVSVHPYPVVRFVNDTSTLCGAL
                     RVKFNNISIFEPGSSFAWDFGDGFSSSIASPTHDY"
BASE COUNT          798 a          623 c          621 g          979 t
ORIGIN      
        1 atagtcatgc gttggtgatg caatacttga agaaaaacca tcgccaaaat cccatgcaaa
       61 actcgagccc ggttcaaata ttgaaatatt attaaactta actcgcaatg cgccacaaag
      121 cgtgctggta tcgtttacaa atctcacaac cggatacgga tgtacactta cattttgaat
      181 tgttgaaact gctgtgcagc cattcagatt tacaatttgc actgtgtagt tacccgaagt
      241 tttaacaaca atatctcgtg tggttgcacc ggtactccat aaataactta accccatact
      301 tgcttgtagt gttacctgtt cgccatcaca aaaacttgta gggccattag gtgatatact
      361 tgcttcggca gttaatactt ctaatttaaa tacatcttca cctgtacatc cattggcatc
      421 ggtaactgtt acggtataat taccggcagt atttatatta ataatctgtg catttaagcc
      481 tgtactccaa acataattta caaaaccatt tccggcatca attaacgata caaaaccaaa
      541 acaaatggtg tcgttaccaa caatttttgg tgtagggttc tgattaactg ttaatacaat
      601 actggtagat gctgagcagc cattattatc tgttactgtt actgtatagg caccggctac
      661 acctgttgta agtatttgtg tagtatttga gccactccat tggtaattgc tataaccact
      721 gcctgcatca aaactgctcg tggtgccatc acaaatggaa gtaatgcctg ttatagccgg
      781 tgtgggcaag gcatttatta ctactacctg cgccactgaa ttggtgcatc cattaccatc
      841 ggtaactgtt accgtataag tgttcgctac ggtaggatta atacttgcag ttgttgctcc
      901 attactccat atataattgg tataacctgc attggtattt aacgtagctg caaagccctg
      961 gcatacggct gttacaccac ttatgtttgg tgtaggcaat gcattaacag ttaaactata
     1021 tgatgcagtt gctgtgcatc cgttaccatc tgttactgtt actgtatagg caccggctgt
     1081 ggttggatta atattctgag ttacgttacc attactccat tggtattggc tgtaaccggc
     1141 accggcatca aaggtagtgg ggtcgttttg acaaataaca tttactccgg ttattgctgg
     1201 tgttgggttt tgatttacaa ccacattaat attagtagaa ccggtacagc catttgcatc
     1261 tgtaacggtt actgtataga tagctgcaac gtttggattt ataatttgtg tgctactgcc
     1321 attactccat tggtagtttg cataacctgc accggcatca atattagcat tgttgccttg
     1381 gcatactact gcctgaccgg taattgttgg tgtaggcaag gcatttatta caactgcctg
     1441 cgcaacggaa ttggtacaac cattaccatc ggtaactgtt accgtatagg tgtttgctac
     1501 ggttggattt atagttgcag ttgttgcacc attgctccaa acataattac tatagccggc
     1561 atttgtattt aacgttgctg caaagccctg gcataccgca gttacaccac ttatgtttgg
     1621 tgtaggcaat gcattaacgg ttaaactata tgatgcagtt gctgtgcatc cgttaccatc
     1681 tgttacggtt actgtatagg caccggctgt ggttggatta atattctgag ttacgttacc
     1741 attactccat tggtattggc tgtaaccggc accggcatca aatgtagtgg ggtcgttttg
     1801 gcaaataaca tttactccgg ttattgaagg agttgggtta ttacttacaa ccacattgat
     1861 attggcagat cctgtacatc catttgcatc agtaacggtt actgtatagt tggctgcaat
     1921 tccggggttt attacttgtg tgctgcttcc atcgctccat tgatataaac tatatccggc
     1981 acctgcattt aagtttgcat tggcaccctg acatactgcg gcaatcccag taattgatgg
     2041 agtgggtagt gcgttaatta caactgcctg ggcaactgaa ctggtacaac cattaccatc
     2101 ggtaactgtt accgtatagg tatttgatac ggttggattt atagttgccg ttaacgctcc
     2161 attactccat acataattgg tatagccggc attggcattt aatgtagctg caaaaccctg
     2221 acatacggcc gttacaccgc ttatgtttgg tgtaggcaat gcgttaacta caagtgtatt
     2281 gtttgcgcta cctgtacaac cattaatatt ggttactgta actgtataag tatttgctgt
     2341 gtttatcagg ttgttttgag tagttactcc attactccac aaatagctgc tgtagcctgt
     2401 acctgcatca aacgaagtat tagcccctgc acaaatggca ttgaggccgg taacaaccgg
     2461 tgtagggttg gcattaacta ccaatgtggc actatttgta ccggtgcatc cattaatgtt
     2521 tgtaactgta accgtataag tacctgctgt tgtaagcaaa ttgctttgtg tagtgctgcc
     2581 attactccat acataactac taaaaccggc cccgccattt aatgtagtgt tggtaccatt
     2641 acaaattgcc ataataccgg taataacggg cgcagggttt gcatttacaa ttacattttg
     2701 cgatacagta gctgtacaac tattgatatc cgtaaccgtt accgtgtaac tacctgcaat
     2761 ggttgtatta attgtagctg ttgttgttcc attgctccac aggtaatcag aaaaacctgc
     2821 atttgcattt agtgttgctg ctataccttg acatacagtg cttgtacctg ttatatttgg
     2881 tacgggcaag gcgttaacaa ttaaagtatt acttgctgtg ccggtacaac cattggcatc
     2941 agtaacagtt actgtatagg tgtttgatgc gtttataata ttattttgcg tagtaacgcc
     3001 attgctccac tggtaattag c
//
LOCUS       DAOMAA010000126         3018 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_25914_length_3018_cov_2.520081, whole genome shotgun sequence.
ACCESSION   DAOMAA010000126 DAOMAA010000000
VERSION     DAOMAA010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25914_length_3018_cov_2.520081"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..217)
                     /locus_tag="PLO59_02505"
                     /pseudo
     CDS             complement(1..217)
                     /locus_tag="PLO59_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011710106.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
     gene            complement(268..1221)
                     /gene="ftsY"
                     /locus_tag="PLO59_02510"
     CDS             complement(268..1221)
                     /gene="ftsY"
                     /locus_tag="PLO59_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018675341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HQU99998.1"
                     /translation="MSFFSKLFSKEKKETLDKGLEKTRENFFTRVSKAIVGKTTIDDE
                     VLDNLEEVLVSSDVGVPTTLKIIERIQKRVARDKYIGSEALNQMLKEEIVDLLSETDT
                     GIENTFTPNKKPYIILVVGVNGVGKTTTIGKLAHQFKSQGNQVLLGAADTFRAAAVDQ
                     LTIWSNRVGVEIVSQGMNADPASVAYDTLQSAISKNYDVAIIDTAGRLHNKVGLMNEL
                     GKIKRVLQKLMPDAPHEVLLVLDASTGQNAIEQAKQFTAATEVNALALTKLDGTAKGG
                     VVIGISDMFKIPVKYIGVGEKMDDLQVFNREAFIDSLFKTQ"
     gene            complement(1321..1473)
                     /locus_tag="PLO59_02515"
     CDS             complement(1321..1473)
                     /locus_tag="PLO59_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010599808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 domain-containing protein"
                     /protein_id="HQU99999.1"
                     /translation="MAKKVVATLKSGKGKEFSKVIKMVKNPSGSYAFKEEVVHNDHIK
                     DFLKEK"
     gene            complement(1506..1688)
                     /gene="rpmG"
                     /locus_tag="PLO59_02520"
     CDS             complement(1506..1688)
                     /gene="rpmG"
                     /locus_tag="PLO59_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HQV00001.1"
                     /translation="MAKKGNRIQVIMECTEHKNSGVAGTSRYITTKNKKNNPERLELK
                     KYNPVLKKMTVHKEIK"
     gene            complement(1730..1963)
                     /gene="rpmB"
                     /locus_tag="PLO59_02525"
     CDS             complement(1730..1963)
                     /gene="rpmB"
                     /locus_tag="PLO59_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013631354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HQV00002.1"
                     /translation="MSKICDLTGKTSIVGNSVSFSNRKTKRRFNPNLQTKKFFIPETG
                     EYIRLKVSTSALRTINKLGISAAIDKAIRKGSL"
     gene            2348..>3018
                     /locus_tag="PLO59_02530"
     CDS             2348..>3018
                     /locus_tag="PLO59_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HQV00003.1"
                     /translation="MQVTYYGHSCFLTEINGARILFDPFITPNELAKHIDINNIRCDY
                     ILISHGHFDHLIDAEVIAKQTGALLVSNWEIVNWYIKKGVTNTHPMNIGGHWMFDFGK
                     VKCINAVHSSSMPDGSYGGQAMGFLIETPEGNFYYSGDTALNYDMKLIGEFKTMDVVF
                     LPIGNNFTMGVDNAIIASDFINCDHVVGLHYDTFGYITINHDEAVEKFDAAGKKLHLI
                     PIGNT"
BASE COUNT          861 a          539 c          516 g         1102 t
ORIGIN      
        1 tgcaaaatgg tatcaatact ttcttgcttg gcattatcaa taaaaccgca agtatttata
       61 ataaccactt cggcttgctt ttagcttgcg actcatgttt tacatcaaaa tcattggcct
      121 ttaattgccc cattaattcc tcgctgtcaa ctaaattttt tgagcagcct aatgttacaa
      181 cattaacact ggtttgttgg gttgattttg ttttcaaagt atatggtttg taaagcgtta
      241 taaataattc atgattttag tgtctggcta ttgggtttta aaaagagagt ctataaaggc
      301 ttcgcggtta aatacctgta aatcatccat cttttcgcca acaccaatgt atttcaccgg
      361 tatcttaaac atatcagaaa tgccaataac aacgccacct tttgctgtac catctaattt
      421 ggttagggct aatgcattta cttcggtagc tgctgtaaat tgtttggctt gttcaattgc
      481 attttgcccc gtacttgcat cgagtactaa aagaacttca tgtggtgcat cgggcatcag
      541 tttttgtaat acccttttta ttttacccaa ttcattcatt aatccaactt tattgtgaag
      601 tcgcccggct gtatcaatta tagcaacgtc ataatttttg cttatggccg attgcagtgt
      661 atcgtatgca actgaggcgg ggtcggcatt cataccttgc gatacaattt caactcctac
      721 acggttactc caaatggtta actgatcaac agcagccgcc ctaaaagtat cggcagcgcc
      781 aagtaacact tgattgccct ggcttttaaa ttgatgcgct aacttgccta ttgtagtagt
      841 tttacccaca ccgtttacac ctacaactaa aataatgtat ggctttttat tgggggtaaa
      901 tgtattctca attcctgtat ctgtttcgct tagtaaatca actatttctt ctttaagcat
      961 ttgatttaat gcttcactgc caatgtattt gtcgcgtgca acacgttttt gtatgcgttc
     1021 aataattttt aaagtagttg gcacgcccac gtctgacgac actaagacct cttccaaatt
     1081 gtccagcact tcatcatcaa tggttgtttt accaactatg gctttcgaaa ctctggtgaa
     1141 aaagttttcg cgtgtttttt ctaagccttt atctaaagtt tcttttttct ctttagaaaa
     1201 caatttgcta aaaaaactca taggattact tataattaat ttaatggttg tggagaggct
     1261 aaaaacaaaa aaagcctcct acgatatcgc aagaagcttt attacatttt agctttaaaa
     1321 ttatttttct ttcaaaaaat ctttaatgtg gtcattgtgt accacttctt ctttaaaagc
     1381 atatgagcct gatgggtttt taaccatctt aatcactttc gaaaattctt tgcctttacc
     1441 ggatttaagt gttgccacta ctttctttgc catgcttggt gcttattttg agttataaaa
     1501 tcagattact taatttcttt atgtacagtc attttcttta aaaccggatt gtacttttta
     1561 agttctaaac gctcggggtt attttttttg tttttagtag taatgtatct tgaagtgcct
     1621 gcaacaccac tgtttttatg ttcggtgcat tccattatca cttgaattct gttgcctttc
     1681 tttgccatcg tttttacttg ttaatcgttt tcattaaata ccgtaataat tataaactgc
     1741 ctttacggat ggctttatct attgccgcac tgatacctaa cttattgatg gtgcgtaatg
     1801 ctgaagtaga taccttaagt ctgatatatt cgcctgtttc gggaatgaaa aacttttttg
     1861 tttgcaagtt cggattaaac ctgcgttttg ttttgcggtt cgaaaacgaa acactgttac
     1921 caacaataga tgttttaccg gttaagtcac aaatctttga cataatgtta tttttaattt
     1981 taacggggtg caaatatatt cttttttgtt ttccaaaaaa caaaattctt gcagttattc
     2041 atattgcatt gttaatacct ttttattcaa ctgtggcaca tgcttttggc ctgtaaatct
     2101 tatttgccag tttaataatt agttattctg ccatttttat tttgtaccaa tagacttaaa
     2161 aaaatgaata agatgccaaa caaacactct gtttgtcaac ttgaggtttc actcaaagca
     2221 ttgaaaaaat tacgtaagct tggcttggaa gaacatgtat gttaacatga acgtgttggt
     2281 aattagcgta caagctttaa agcaagcaat ttaatatcgg taattttata acgttaaaaa
     2341 tttatttatg caagtaacgt attacggcca ttcctgtttt ttaactgaaa ttaatggtgc
     2401 acgtatttta ttcgatcctt ttattacgcc caatgaactg gcaaaacata tagatattaa
     2461 caacattcgt tgcgattata tactaatttc tcacggccat tttgatcatt taattgatgc
     2521 cgaagtaatt gccaagcaaa ccggtgcatt attggttagc aattgggaaa ttgtaaattg
     2581 gtacataaaa aaaggtgtta ccaatacgca ccccatgaat ataggaggcc attggatgtt
     2641 tgattttggg aaggtaaaat gtataaatgc agttcatagc agctccatgc ccgatggtag
     2701 ttatggtggt caggcaatgg gttttttaat tgagacacct gaaggaaatt tttactactc
     2761 gggcgataca gcattaaatt acgatatgaa actgataggg gagtttaaaa cgatggatgt
     2821 ggtttttttg cccattggca ataattttac catgggtgtt gataatgcca taattgcttc
     2881 tgactttatt aactgtgatc atgttgttgg gcttcactac gatacttttg gatacataac
     2941 tataaatcat gacgaagctg ttgagaaatt tgatgctgcc ggtaaaaaac tacaccttat
     3001 acctattgga aacaccat
//
LOCUS       DAOMAA010000127         3017 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_25924_length_3017_cov_2.338285, whole genome shotgun sequence.
ACCESSION   DAOMAA010000127 DAOMAA010000000
VERSION     DAOMAA010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25924_length_3017_cov_2.338285"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="PLO59_02535"
     CDS             complement(<1..443)
                     /locus_tag="PLO59_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein]--UDP-N-
                     acetylglucosamine O-acyltransferase"
                     /protein_id="HQV00004.1"
                     /translation="MHSPLAVISKQAKIGNHVTIGPFCVIHDDVVIGDNCNLHSHVVI
                     YDGARIGNNNEFYPGAVVSAVPQDLKYKGETTYTFIGDNNTIREFVTINKGTVALGKT
                     VIGDRNLLMAYVHVAHDCVVGSDCILANGVTLAGHITIDDFAIIGG"
     gene            complement(589..1977)
                     /locus_tag="PLO59_02540"
     CDS             complement(589..1977)
                     /locus_tag="PLO59_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HQV00005.1"
                     /translation="MLQTTLKNEVTIEGVGLHTGNAVSITFKPAPENHGIKFKRIDLP
                     GSPKVDADVDNVVDTSRGTTLEQNGARIYTTEHALAALAGLEVDNVLCEINAPELPIM
                     DGSAQPFVDKILLAGIETLKQERVYYTLTENLTYEDVERKVEMLAVPSEEFRVTVMVD
                     YNSEILGTQHAQIHQMQEFKDQIARCRTFVFLHELKQLAKFNLIKGGNVDNAIVLVDH
                     LIPQEELDELATLLNKPKIKIEEKGVLNNIKLHFQNEPARHKLLDIVGDFALIGVPLK
                     AHILAARPGHKANTEFARKIKDLIKKEKNIVRIPVHDPNKTPAFDINKIASYLPHRIP
                     FLLIDKILEIGADYVVGLKNVTMNEWFFQGHFPNNPVMPGVLLIEAMAQVGGILALSS
                     VPDPDNHWTYFMKIENARFKKMVLPGDTIIFDLKLKSPIRRGIVQMTGVAYVGDKAVM
                     EAEMMAQIVRKS"
     gene            complement(2026..>3017)
                     /gene="lpxD"
                     /locus_tag="PLO59_02545"
     CDS             complement(2026..>3017)
                     /gene="lpxD"
                     /locus_tag="PLO59_02545"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019388632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HQV00006.1"
                     /translation="LLQGKVEGNPETWVNKLSKIEQGEAHSLSFLANPKYTPYIYNTA
                     ASIVIVSQNFIAEQPISATLVRVPDAAAAFSKLLEMYNTFKQNKTGIEQPVFVDSTAS
                     IGEDVYIGAFAYIGKNVTLADGVKVYPHTYIGDDVTIGKNTVIYAGAKIYNGCNIGNK
                     CIIHSSVVIGADGFGFVPNAQGGYDKNPQTGNVVIHDDVEIGANTTIDRATLGSTIIG
                     KGVKLDNLIQIAHNVEIGENTVIAAQTGIAGSTKIGKNCLIGGQVGIVGHITIADGTK
                     VQAQSGISRPVTETNTLLQGSPAYNMSEYKRNYVHFKNFDTIVKRINDLESNK"
BASE COUNT          790 a          641 c          575 g         1011 t
ORIGIN      
        1 cccccgataa tggcaaaatc atcaatggta atgtggccgg ctaaggtaac gccattggct
       61 aaaatacaat cgctgcctac cacacaatcg tgggcaacgt gtacatatgc catcagcaag
      121 tttctatcac ctatcaccgt tttgcccaat gctacggtac ccttgtttat agttacaaac
      181 tcacggattg tattgttatc gccaataaaa gtgtaggtgg tttcgccttt atattttaaa
      241 tcctgaggca ctgccgatac aacggccccc ggataaaatt cgttattgtt accaatacgt
      301 gcgccatcgt aaataactac atggctatgc aggttgcagt tatcgcctat tactacatca
      361 tcgtgtataa cacaaaaagg gccaatggtt acatggtttc ctatttttgc ttgcttactg
      421 attacggcaa gtggcgaatg catagttgtt tgttttagta tcgtatcaac aatacgatgt
      481 catattgtat ggggctacaa ataaagtgtt ttatatggca tgacaatgtt ttgaatttgt
      541 gcattcttat tttgctgcta acaaataaaa tttacctgct ttttgcttct agcttttgcg
      601 cactatttgg gccatcattt cggcttccat aactgcttta tcgcctacat aagccacacc
      661 ggtcatttgt acgatgcccc tgcgtatggg tgatttcagt tttagatcga aaatgatggt
      721 atcgcccggc aaaaccattt ttttaaatcg ggcattttca attttcataa aatacgtcca
      781 atggttatcg gggtcgggca cactgcttaa tgctaaaatg ccgcctactt gtgccattgc
      841 ttcgataagc agtacaccgg gcattaccgg attgttcgga aagtggcctt gaaaaaacca
      901 ctcattcatg gtaacatttt ttaaccctac cacatagtcg gcacctattt ccaatatttt
      961 atctatcaat aaaaatggaa tacggtgcgg taaataggat gcaattttat ttatgtcgaa
     1021 agccggtgtt ttgttagggt cgtgcaccgg aatgcgtact atgttctttt ccttttttat
     1081 taagtcttta atcttacgtg caaattcggt attggcttta tggcctggcc ttgcagctaa
     1141 aatatgtgct tttaacggaa cgcctattaa agcaaaatcg cctacaatat ccaacagttt
     1201 atggcgtgca ggctcgtttt gaaaatgcag ctttatatta tttagcacac ccttttcttc
     1261 gatttttatt ttgggtttat ttaaaagtgt ggccaactca tccagctctt cttgtggtat
     1321 taaatgatca accaatacga tggcattatc cacattgccg ccttttatta aattaaactt
     1381 tgccagttgt tttaactcat gcaaaaacac aaacgtacgg cagcgtgcaa tctgatcttt
     1441 aaattcttgc atctggtgta tttgtgcatg ctgtgtgcct aaaatttcgc tgttgtaatc
     1501 aaccattacg gttactctaa actcttcgct gggtacagcc aacatttcaa ctttacgttc
     1561 tacatcttca taggtaaggt tttcggttag tgtatagtac acacgctctt gtttcaacgt
     1621 ttcaatgcca gccaacaaaa tcttatctac aaatggctgc gcactgccat ccataatggg
     1681 caattcgggc gcattaattt cgcatagtac attatcaacc tccaaacctg ctaaggcagc
     1741 taatgcatgc tcagtagtat aaatacgggc accgttttgt tctaatgtgg tgccgcggct
     1801 ggtatctaca acattatcaa catcggcatc aactttgggg ctgccgggta aatcaatgcg
     1861 tttaaatttt atgccgtggt tttcaggtgc aggtttaaac gttatactaa ccgcattgcc
     1921 ggtatgcagg ccaacgcctt ctatagtaac ttcgtttttt aatgtagttt gtaacatagg
     1981 ttttggttac ggagttaaaa ttactttgta taaatgtgta tttgattact tgttactttc
     2041 taagtcattt atgcgtttaa caatggtgtc gaaattttta aaatgtacat agttacgttt
     2101 gtactcactc atgttatatg ccggtgaacc ttgcagcagt gtattggttt cggttaccgg
     2161 acgcgatata ccgctttgtg cctgcacctt ggtgccatcg gctatggtta tatgacctac
     2221 aataccaacc tgcccgccaa ttaaacaatt tttaccaatt ttagtgctgc ctgctatgcc
     2281 ggtttgcgct gcaataaccg tgttttcgcc tatttcaacg ttatgtgcta tttgtatcag
     2341 gttatctaac ttaacgcctt tgccaataat ggtgctgccc aatgttgccc tgtcgatagt
     2401 agtgttagcg cctatttcaa catcatcgtg tatcacaaca ttgcctgttt gcggattttt
     2461 atcgtagcca ccctgtgcat tgggtacaaa gccaaaacca tcggcaccaa ttacaacact
     2521 gctatgtatg atgcatttat taccaatatt acaaccgttg taaattttgg cacctgcata
     2581 tatcacagtg ttttttccaa tggttacatc atcgccaatg tatgtgtgcg gataaacttt
     2641 aacgccatcg gctaaggtta catttttgcc aatgtatgca aatgcaccta tgtaaacatc
     2701 ctccccaata cttgccgtgc tatcaacaaa aaccggttgc tcaatgcctg ttttgttttg
     2761 tttaaaagtg ttatacattt ccaacaactt gctaaaagct gcggctgcat cgggcacacg
     2821 taccagcgta gctgaaatgg gttgctcggc aataaagttt tgacttacaa ttacaatact
     2881 ggctgctgta ttatagatgt agggtgtata ttttggattg gctaaaaagc ttaatgaatg
     2941 agcctcgcct tgttcaattt tcgaaagctt atttacccaa gtttcgggat tgccttcaac
     3001 ttttccttgc agtaatt
//
LOCUS       DAOMAA010000128         3014 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_25965_length_3014_cov_2.837445, whole genome shotgun sequence.
ACCESSION   DAOMAA010000128 DAOMAA010000000
VERSION     DAOMAA010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25965_length_3014_cov_2.837445"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            34..711
                     /gene="rsmI"
                     /locus_tag="PLO59_02550"
     CDS             34..711
                     /gene="rsmI"
                     /locus_tag="PLO59_02550"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HQV00007.1"
                     /translation="MSTLILVPTPIGNLQDITLRAIETLKQCDYLLAEDTRNTIFLLK
                     HLGIEKKVYAYHAHNEHKQMEAIIAHIKMASMVALVSDAGTPGISDPGFLLVRTCIEN
                     QIKIETLPGATALIPALVNSGLPCHRFCFEGFLPQKKGRQSRLQELTAETRTMVFYES
                     PYRLVKCLQQFAEVFGAERMCSVSRELTKMFEENYRGTLQQALAHFEGKAIKGEIVIV
                     VAGFDEG"
     gene            complement(819..2108)
                     /locus_tag="PLO59_02555"
     CDS             complement(819..2108)
                     /locus_tag="PLO59_02555"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV00008.1"
                     /translation="MCGNATIRLGYGTFRVDMSQTTLCPGGPHIAGSFNGWNASATPM
                     TAIGNGIYEVVLMLDTTQSYQYKFLKDSTWACGDETVPAACAVNNNREVVVPEHDTIL
                     PTVCFGSCAPCQIVPNPTVDVTFRVDVSQNPCTGGGPHLVGSFNGWNATATPMTAVGA
                     GVYEATVSLDTTASVQYKFLNDSTYSACGNENVPAACGVPNGFGGFNRQLEVPEINST
                     LSTVCFSSCAACIPPGMVNVTFRVDMAFTTLGPGGPHVAGTFNSFNAAATPMTNVGGS
                     VYAATIMLDTTATVEYTFLKDSTYIDQEVITALCGVPNGFGGFNRQLFVPEADSTLIA
                     YCYSSCFTCNVGINDVSKSQLIMYPNPAGNFITIELPLQTQAGLLLITNATGQVVVKQ
                     KVTGLKQQIDISTLPIGVYAVQSITDNKLAVSKLIKQ"
     gene            complement(2054..>3014)
                     /locus_tag="PLO59_02560"
     CDS             complement(2054..>3014)
                     /locus_tag="PLO59_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00009.1"
                     /translation="SATATPMTNIGNNVYETAITINSGLAIEYVFLKDSSFNGQEQVP
                     AACGVPNGFGGFNRALTVPINDTTLQTVCFSSCSSCPPIVDVILRVDMSNETIAPGGV
                     HVVGSFNNWSATATPMQHIGNNVYRKKITQPAGTNLLYTFLKDSTYNGQEAVPAACGV
                     PNGFGGFNRNYTVPTNNSTLPIVCYSSCTTCVAVVPSDSVMVTFSVDMSQTTLCPGGP
                     HIAGSFNNWSPTATPMVAVGNGVYEVTLQLDSTLSYQYKFLKDSTWACGDETVPAACA
                     VSNNREVVVPETNYAIPTVCFSSCAPCVVTPPSDSAMVPSGLI"
BASE COUNT          868 a          650 c          642 g          854 t
ORIGIN      
        1 ccggttttgt atgcattgtt ataacttatt gttatgtcaa cattaatatt ggtaccaaca
       61 cctattggca atttacagga tataaccttg cgtgcaattg aaacattaaa gcaatgcgat
      121 tacctattag ccgaagacac cagaaatacc atatttttat taaaacatct gggcatcgaa
      181 aaaaaagtat atgcctatca tgcacataat gagcataagc aaatggaggc cattattgcg
      241 catataaaaa tggcatcaat ggttgcattg gtaagcgatg caggcacacc gggtattagc
      301 gaccctgggt ttttattggt gcgtacctgt atcgaaaatc aaattaaaat tgaaacgctg
      361 cccggtgcaa cagcactaat tccggcattg gtaaattcgg gtttgccatg ccaccggttt
      421 tgtttcgaag gttttttgcc gcaaaaaaaa ggacgtcaat cgcgcttaca ggaattaact
      481 gccgaaacac gtaccatggt tttttacgaa tcgccttaca ggcttgtaaa gtgcttgcaa
      541 caatttgccg aagtgtttgg tgccgagcgt atgtgcagtg tatcgcgtga gcttacaaaa
      601 atgtttgaag aaaactatcg cggtacgttg caacaagccc ttgcacattt tgaaggcaaa
      661 gccattaaag gcgaaatagt aattgttgtg gcaggttttg atgaaggata aattacacct
      721 tgcctttaca agtggtttga ttaaagttag tttcatttaa aaaaaagcgg ctgttacaat
      781 ttaatataac agccgctttt tgtttttgtt gtaaccgttt attgcttaat caatttgctt
      841 acggccaatt tattgtcggt tatagattgt acggcataca caccaatggg caatgtgcta
      901 atatctattt gttgttttaa gcctgtaacc ttttgtttaa ccacaacctg acctgttgca
      961 ttggtaatta ataacaagcc cgcttgggtt tgcaatggca gttcgatggt aataaaatta
     1021 ccggccgggt ttggatacat aatcaattgc gatttgctaa catcgttaat gcctacgttg
     1081 catgtaaaac aactgctgta acaataggca attaaagtac tgtcggcctc gggcacaaat
     1141 aactggcggt taaaaccacc aaaaccgttg ggcacaccac aaagtgccgt aataacttcc
     1201 tggtcaatat aggttgagtc tttaagaaaa gtatattcaa ccgttgctgt agtatccaac
     1261 ataatggtgg cggcataaac acttccacca acatttgtca tgggtgtagc tgcagcatta
     1321 aagctgttaa atgtaccggc aacatgtggg ccgcccgggc ctaaggttgt aaaggccata
     1381 tcaaccctaa acgttacatt aaccataccc ggaggtatgc atgctgcaca actgctaaag
     1441 caaacggttg agagtgtgct gtttatttca ggcacttcta attggcggtt aaaaccacca
     1501 aaaccgttag gcacaccaca ggcagcaggt acgttttcat taccacatgc gctgtaagta
     1561 ctgtcgttta aaaacttgta ttgtacggaa gctgttgtat ctaaacttac cgttgcttcg
     1621 taaacacctg cacccacagc agtcatgggt gttgctgttg cattccatcc attaaacgaa
     1681 cctactaaat gcgggcctcc accggtgcat gggttttggc taacatctac cctaaacgta
     1741 acgtcaacgg ttgggttagg tacaatttga cagggcgcac aacttccaaa acaaacggta
     1801 ggtaaaatgg tatcatgctc aggaactact acttcgcggt tattgtttac agcgcaagct
     1861 gccggtacgg tttcgtcacc acaagcccag gtactgtctt ttaaaaattt atactgatag
     1921 ctttgtgtgg tatccaacat caatactact tcgtaaatgc cgttaccgat ggctgtcatt
     1981 ggtgtagcgc ttgcattcca tccgttaaac gagccggcaa tatgcgggcc accggggcat
     2041 aatgttgttt ggctcatatc aaccctgaag gtaccatagc cgagtcggat ggtggcgtta
     2101 ccacacatgg tgcacaactg ctaaaacaaa ccgttggaat tgcataattg gtttcgggca
     2161 caaccacttc gcgattattg cttacggcac aagcagccgg tacggtttcg tcaccacaag
     2221 cccaagtact gtcttttaaa aacttatatt gataacttaa ggtactgtct aattgtaacg
     2281 ttacttcata tacaccatta ccaacagcaa ccataggcgt agccgttggc gaccaattat
     2341 taaacgagcc tgcaatgtgt gggcctcccg gacataaggt agtttggctc atatctacac
     2401 taaaagttac cataaccgag tcggatggta ccacagcaac gcaagtggta caactgctgt
     2461 agcaaacaat tggtagtgta ctgttattgg tcggtaccgt ataattacga ttaaagcccc
     2521 caaatccatt gggcacacca catgctgccg gaacagcttc ttgtccgtta taagtactgt
     2581 cctttaaaaa agtgtaaagc aaattagtgc cggccggctg cgtaattttt ttacgatata
     2641 cgttattgcc aatatgctgc atgggcgttg ctgtagcact ccagttgtta aagctaccca
     2701 ctacatgtac accaccgggc gcaatggttt cattgctcat atcaactctt aaaattacat
     2761 caactatagg tggacaactg ctgcaactgc tgaagcaaac tgtttgtaaa gttgtatcgt
     2821 ttatgggtac cgttagcgca cggttaaaac cgccaaatcc gttaggcacg ccacatgcag
     2881 ccggaacttg ttcttgacca ttaaacgatg agtcttttaa aaatacgtac tcaatagcaa
     2941 ggccgctatt aatcgtaatg gctgtttcgt aaacattgtt gccaatatta gtcatggggg
     3001 tagcggttgc actc
//
LOCUS       DAOMAA010000129         3008 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_26067_length_3008_cov_3.105317, whole genome shotgun sequence.
ACCESSION   DAOMAA010000129 DAOMAA010000000
VERSION     DAOMAA010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26067_length_3008_cov_3.105317"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="PLO59_02565"
     CDS             complement(<1..443)
                     /locus_tag="PLO59_02565"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HQV00010.1"
                     /translation="MDIALYISALTIGFLGSFHCVAMCGPIALVLPGKKDSKTAMIPG
                     RVFYNLGRIVTYILLGLILGFIGFSISIKGFQRELSILTGVVILISVFFTSGGKHIPF
                     LSSGIVKLTAGLKQYFKKLFGNGSSLSLFAIGLLNGLLPCGLVYLS"
     gene            complement(446..883)
                     /locus_tag="PLO59_02570"
     CDS             complement(446..883)
                     /locus_tag="PLO59_02570"
                     /inference="COORDINATES: protein motif:HMM:NF017559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HQV00011.1"
                     /translation="MKFNWGHGVALAYIVFAASMIWFAVKASQQTYDLVDEHYYEKAV
                     GYQEIISAQKNGQLEKLLINYLPENHSIVLTTDSNLTNLNGTLAFYKPDNATADFNLN
                     FSIDSTGTVSIPLKQLAHGYWNINATWIAAGKTCYKEKRIFIE"
     gene            complement(890..2287)
                     /gene="ccoG"
                     /locus_tag="PLO59_02575"
     CDS             complement(890..2287)
                     /gene="ccoG"
                     /locus_tag="PLO59_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011584531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HQV00012.1"
                     /translation="MDAINNDESFRDSISTITAEGKRAWLYPKKPKGKLYNARTWVSY
                     GFLIIFFALPWIQINGHPFMLFNVIERKFVLLGIAFFPQDFFLFVLAMLTFVVFIALF
                     TALFGRLWCGWACPQTVFMEMVFRKIEYWIEGDMMQQKALDKRPWDTYKATRKISKHL
                     IFFFISFVISNTFLQYIIGVDDWLKIVTDDLSQHKAGFISILVFTGIFYGVYARFREQ
                     VCIVVCPYGRLQGVLLDPNSVVVGYDYKRGEPRGKLTKSVENKLGDCIDCHQCVQVCP
                     TGIDIRNGTQLECINCTACIDACDDIMDKIKKPHGLIRYTSENSIKQGIKFKITPRII
                     GYSAVLFVLLAALSVFLLIRTDIETTVLRTPGLLYQKTDSLHVSNLYNIEMVNKTFDD
                     MPIELKVVEPKGEIKWVGNGITKLKQQSISQSVFFLIIPKSEIIKTKTKVKFEVYSNG
                     KRIDKTETNFLGPNN"
     gene            complement(2301..>3008)
                     /locus_tag="PLO59_02580"
     CDS             complement(2301..>3008)
                     /locus_tag="PLO59_02580"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HQV00013.1"
                     /translation="ANATVAVAEEKAPGFFRKIYLRMVDSVPVSKEKDIVLDHDYDGI
                     KELDNNLPPWWKYGFYLTIVFSFLYMFVYHVTGTGKLQLAEYNDELHAAQLAKAERMK
                     SSAENITEDNVVALKETDVLAKGKDSYDKLCAACHRADGGGQVGPNLTDEFWLHGGGI
                     KNIFHTITYGVPDKGMISWQSQLSPKQIQYVASYLMTMQGTKPAGPKEPQGEKWTEVA
                     ATTADSTTTATADSLSK"
BASE COUNT          924 a          601 c          552 g          931 t
ORIGIN      
        1 gatagataaa caagtccaca tggtaataaa ccatttaaca agcctattgc aaagagcgaa
       61 agtgaagagc cattaccgaa taatttttta aagtattgct ttaatcccgc ggttaacttt
      121 acaataccgg aacttaaaaa gggtatgtgc tttccgcctg aagtaaaaaa aacagatatt
      181 aaaattacta caccggtaag aatgcttagc tcgcgttgaa agccttttat tgaaattgaa
      241 aaaccgataa atcctaaaat caacccaagc aaaatataag ttacaatgcg gccaaggtta
      301 taaaatacgc ggcctggaat catggccgtt ttcgaatcct ttttgcccgg taataccaaa
      361 gcaatagggc cacacatggc aacacagtgg aaactaccta aaaagccaat agtaagtgct
      421 gatatatata aggcaatgtc catctttatt cgataaaaat tcttttttct ttatagcatg
      481 tcttgccagc agcaatccat gttgcattaa tgttccaata accgtgtgca agttgtttta
      541 acggaattga tacagtgccg gtagaatcaa tagaaaaatt taaattaaaa tcagcggttg
      601 cattatcggg tttgtaaaaa gcaagggtgc catttaaatt cgttaagttt gagtccgttg
      661 ttaatacgat tgaatgattt tcgggcaaat agttaatcaa tagtttttca agttgcccat
      721 ttttttgtgc agaaattatt tcctgatagc ctactgcttt ttcataataa tgttcgtcaa
      781 ccaaatcata agtttgttgc gatgctttta cagcaaacca tatcatactt gcggcaaaca
      841 caatataggc gagcgcaacg ccatgacccc agttaaattt catattacat taattattag
      901 gtccgagaaa attggtttct gttttatcaa ttctttttcc attgctatac acttcaaatt
      961 ttactttggt tttagtcttt attatttccg attttggaat aattaaaaag aaaacacttt
     1021 gtgatataga ctgttgcttc agttttgtaa taccattacc aacccatttt atttcgccct
     1081 tgggttcaac aactttaagt tcgataggca tatcgtcaaa agttttattt accatttcaa
     1141 tgttgtaaag attactaaca tgcaggctgt cggttttttg gtatagtaag ccgggtgtac
     1201 gcagcacggt ggtttcaata tcggttcgta ttaataaaaa cacactcaaa gcagcaagca
     1261 gtacaaaaag tacggcacta taaccaataa tgcgtggggt tattttaaac ttaatgcctt
     1321 gtttaatgga attttcgctg gtataacgaa ttaagccatg tggctttttt attttatcca
     1381 ttatatcatc gcaggcatca atacaggccg tacaattaat acattctaac tgtgtgccat
     1441 ttcgtatatc aatgccggta gggcatacct gtacacattg gtggcaatca atgcaatcgc
     1501 ccaatttatt ttcaacgctc ttagttagtt tgccacgcgg ttcgccacgt ttataatcgt
     1561 aaccaactac aactgaattg gggtcgagca gcacaccttg caaacgacca taaggacaaa
     1621 ccacaatgca cacctgttcg cggaaacgtg cataaacgcc ataaaaaatg ccggtaaaaa
     1681 ccagtatgga aataaagccg gctttatgtt gtgataaatc atcggttaca atttttaacc
     1741 aatcatctac tccgattata tattgaagaa aggtgttgct aataacaaat gatatgaaaa
     1801 agaaaatgag gtgtttgcta atttttcgtg ttgctttata ggtgtcccac ggtcttttat
     1861 caagagcttt ttgctgcatc atatcacctt caatccaata ttcgatttta cgaaacacca
     1921 tttccataaa cactgtttgc gggcatgccc agccgcacca aagcctgcca aacaatgcag
     1981 taaacagagc gataaaaaca acaaaggtga gcatggccaa aacaaacaga aaaaagtcct
     2041 gcggaaaaaa tgcaatgccc agcaatacaa atttgcgttc gataacattg aacagcataa
     2101 atggatgccc gttaatttga atccaaggca atgcaaagaa aataatgagg aaaccatagc
     2161 taacccaagt gcgtgcattg taaagcttac cctttggttt tttaggatac agccaggcac
     2221 gttttccttc ggcagttatg gtcgaaatac tgtcgcgaaa cgattcgtca ttatttattg
     2281 catccaaaat gttcaggttt ttattttgat aatgaatctg ctgttgctgt agttgttgag
     2341 tcggctgtgg ttgctgctac ttctgtccat ttttcgcctt gtggttcctt aggcccggca
     2401 ggcttagttc cttgcatggt cattaaataa ctggcaacat attgaatttg ttttggcgat
     2461 aactgcgatt gccagctaat catgcctttg tcgggcacac cgtacgtaat agtgtgaaaa
     2521 atgtttttta tgccaccacc atgcagccaa aattcgtccg taaggttggg gcctacttgt
     2581 ccgccaccat cagcccgatg gcatgctgcg caaagtttat catagctatc ctttcctttt
     2641 gcaagcacat cagtttcttt caaagcaact acattatctt cggtaatgtt ttcggcactt
     2701 gatttcatac gttctgcttt cgccaattgt gcagcatgca actcatcatt gtattcggcc
     2761 aactgcagtt tacctgtgcc cgtaacatga tacacgaaca tatatagaaa tgaaaatacg
     2821 atggtaagat aaaagccata tttccaccag ggtggtaaat tgttgtcaag ttcttttatt
     2881 ccgtcataat catggtcgag cacaatatct ttttcttttg atacgggcac actatcaacc
     2941 atgcgtaaat atattttccg aaaaaatccg ggtgcttttt cttcggctac agccacagtg
     3001 gcatttgc
//
LOCUS       DAOMAA010000130         3003 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_26143_length_3003_cov_2.842944, whole genome shotgun sequence.
ACCESSION   DAOMAA010000130 DAOMAA010000000
VERSION     DAOMAA010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26143_length_3003_cov_2.842944"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="PLO59_02585"
     CDS             complement(<1..251)
                     /locus_tag="PLO59_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HQV00014.1"
                     /translation="MKNYNNHHYNKDLREFTRELRSERPTKAERRIWKSLLCKKQSEI
                     RFLRQRPISYFIVDFFAPELGLIVEIDGNSHYHKPEYDA"
     gene            complement(467..2575)
                     /locus_tag="PLO59_02590"
     CDS             complement(467..2575)
                     /locus_tag="PLO59_02590"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV00015.1"
                     /translation="MKKIKQLLFTVLVTTFIFGYYQSKAQIVFDFENRDISLWGIEGD
                     GSILIDTINGNPGSVLRVEEPATGASNFVIVHPAANGNWSLADSTDFISWDLLVHKNA
                     GTMFSANNDLIELSGPGGYAKFTGIYSMLQDTFIHRTVALNPALWTMVSGTWTALMQS
                     VDLIRVRTEFVDGDEYDLLDNVALSFTPIINEPEGYVCSPFDSLDGFDGWNFVNTASI
                     ASVNTFGNPPNAIKINDKSNVLSYGYAPPKFRGNFHTLNDTGTISFDVRVNTSLTTLS
                     LPPHFIRLAGPGGQATFPITAADAAAMKNVWHSFSIPVVDSLWTITSGTWNALLNNVT
                     VIEIALEYYNGTSETVYFDNFCIGSFTPPCHAEGGNITPATATFCAQQTYVMTATGAS
                     SHPDNIYQWQRATVSGGPYTNVTGGSGANTTSFTTGKLIAGTYYYVLKVTCPDGLTDY
                     SNELTVNVKALPTASITAESDTIFCSGGSVVLNAPVSANRAYQWLRSGNIISGANTAS
                     YTATTSGTYKVEVTNTISGCTKSSYGIKVTTNPLPTATVTPAGPLTFCAGDSVVLQAN
                     AGAGFIYQWKKEAGYITGATQQNYTASTGGKYKIQITDSNGCTRTSDPVTVTVNCRNG
                     IAISTNAVSVYPNPATDNMVIENIPASDYIIEVINVLGEVVLSQKLNPGSNSVSVEHL
                     TSGVYVIKTNLGESLKFIRK"
     gene            complement(2729..2920)
                     /locus_tag="PLO59_02595"
     CDS             complement(2729..2920)
                     /locus_tag="PLO59_02595"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HQV00016.1"
                     /translation="MREKEVLQLLTKGKSYKMIGNEMHISYETVHSHIRKIYQKLQVN
                     SVGEAVSKTISKNILKTLL"
BASE COUNT          893 a          576 c          571 g          963 t
ORIGIN      
        1 tatgcatcat attccggttt gtggtaatga gaattgccat caatttctac tatcagtccc
       61 aattccggtg caaaaaaatc tactataaaa taactgatag gtcgttggcg gagaaaacga
      121 atttcagact gttttttaca gagtagagat ttccatattc ttcgttcggc tttggttggt
      181 ctttctgacc ttaattctct tgtaaattca cgtaaatctt tgttgtaatg gtggttgttg
      241 taatttttca tattcgttaa gttacacaaa aagaagaatt aaaacaaata atgaaaagtt
      301 aaaaatttta tttttttaat gaaggaagaa ctatatggtc accctcctta atcctccctc
      361 aagggaggag attaaaaaaa accttgaatc tatatgatcc aaggttttct tatttactta
      421 ctacacttta acacgacaaa gaagcatttg ctcctttgaa aaaaagctat ttgcgaataa
      481 atttcaaact ttcgcctaaa ttagttttaa taacataaac acctgaagta agatgttcta
      541 ccgaaacact gtttgaacct ggattcaatt tttgagataa aaccacttcg cctaaaacat
      601 ttataacttc aattatataa tcggatgccg gtatgttttc tataaccata ttatcagtgg
      661 caggattagg gtaaactgat acagcattgg tactaattgc tataccgttc cggcaattta
      721 ctgtaacagt aaccggatcg gatgtgcgcg tacaaccatt gctgtctgtt atctgaattt
      781 tatatttacc accagtactt gcagtatagt tttgttgtgt tgccccggta atatagccgg
      841 cttccttttt ccattgataa ataaaacctg cacctgcatt agcttgcaat acaacactat
      901 cgcctgcaca aaaagtaagt gggccagccg gtgtaacggt tgctgttggc aaagggttag
      961 ttgttacttt tataccataa gatgatttgg tgcaaccgga gattgtattt gtaacctcta
     1021 ccttatacgt acctgatgtt gtagcagtat aacttgcagt attagctccg ctgataatgt
     1081 ttccgctgcg caaccattgg taagccctgt ttgccgatac tggtgcattt aatacaacac
     1141 tgcctccaga acaaaatatg gtatcgcttt cagcagtaat gcttgcagtt ggtaatgctt
     1201 ttacatttac cgttaattcg tttgaataat cagttaaacc atcagggcag gtaactttta
     1261 aaacataata gtatgtgccg gcaattaact tgcccgtggt aaaagaagtt gtatttgcac
     1321 cactgcctcc ggtaacatta gtgtatgggc ctcccgatac agttgcacgc tgccattgat
     1381 aaatattgtc gggatgcgat gaagctccgg tagcagtcat tacataagtt tgctgtgcac
     1441 aaaaggttgc agtggcaggc gttatgttgc cgccttcggc atggcaaggt ggtgtaaaac
     1501 tgcctatgca gaagttatca aaataaacag tttctgatgt tccgttgtaa tactctaaag
     1561 caatttctat aactgtaaca ttgttaagca aagcgttcca tgtgcccgaa gtgatggtcc
     1621 acaaactatc aacaaccgga attgaaaacg aatgccaaac atttttcatt gcagcagcat
     1681 cggcagcagt tatcggaaaa gttgcttgcc cgcccgggcc agccaaccga ataaaatgtg
     1741 gtggcaagga taaagttgta agcgatgtat taacgcgtac atcaaatgaa attgtacccg
     1801 tatcattcaa tgtatgaaag ttgcctctga acttaggtgg tgcatagccg tatgacaata
     1861 catttgattt gtcgttaatt ttaattgcat ttggcggatt accaaatgta ttaacagagg
     1921 caatagaagc tgtattgaca aaattccatc catcaaaacc atccaatgag tcgaaaggag
     1981 aacacacata tccctctggt tcattgatta tgggcgtaaa cgatagcgcc acattgtcca
     2041 gcaagtcata ctcatcgcca tcaacaaatt cggttctgac tctgattaaa tctaccgact
     2101 gcattaatgc tgtccatgtt ccgctaacca tagtccaaag agccggattg agtgccactg
     2161 ttctgtgtat aaaagtatcc tgaagcatgg agtaaatacc cgtaaactta gcataacctc
     2221 ccgggccgct taactcaatt aaatcgttat tggccgagaa catagtgccg gcatttttgt
     2281 gtaccaacaa atcccacgat ataaaatcgg tactgtcggc aagcgaccaa ttgccatttg
     2341 ctgccggatg cacgattaca aagtttgatg cacctgttgc cggttcttct acacgtaata
     2401 cactgccggg atttccgtta atggtatcta tcaagataga accatcgccc tcaatacccc
     2461 aaagtgaaat gtcgcgattt tcgaaatcga atacaatttg tgctttactt tggtagtatc
     2521 cgaatatgaa ggttgttaca agaaccgtaa ataatagttg tttgattttt ttcatttcta
     2581 ttttatttaa aatggttgtt attggttact gcttattgca tgtgatttaa ttttgggttc
     2641 atttcaagcc ggtaaaaatc cgtgtacaaa actcatataa tagtatgtat taccctatca
     2701 ccaattatgg tgaaaattgc atcgtttact atagtaaagt ttttagaata ttttttgaaa
     2761 tggttttaga aactgcctcg ccaactgagt ttacctgtag cttttgataa attttacgga
     2821 tatgcgaatg aacggtttcg taactaatat gcatttcatt gccaatcatt ttataagatt
     2881 ttcctttggt taaaagctgc aacacttctt tttctcgaat actcaggtta tagttttcgt
     2941 ttggttattg tgtgggttgt aatttggtaa actgatttaa aaccttgcgt acaataacag
     3001 gcg
//
LOCUS       DAOMAA010000131         3000 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_26192_length_3000_cov_2.674703, whole genome shotgun sequence.
ACCESSION   DAOMAA010000131 DAOMAA010000000
VERSION     DAOMAA010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26192_length_3000_cov_2.674703"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..1708
                     /locus_tag="PLO59_02600"
     CDS             134..1708
                     /locus_tag="PLO59_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00017.1"
                     /translation="MNLLNQPTVAEVLTQLSRILAFKTFKNSSILSTFLDYIVRTKLA
                     GNAIQLKEYTIGVAVLLKKADYNPQTDAAVRIHASRLRKALDEYYSIETHAVELFISI
                     PKGSYIPYFEWRINSDKGFVNQTEFEGVKPSIALLPFHAYDETLLPLADGICDQICTE
                     LTNYNELLVKAYFNSRHVATQVSDIKSAGYLLDCNYLITGTIQTDGMFLRCRVQLIRT
                     HDQQQLWASAYEKKMAEFNTFEIQDDIVLHVVNNIAGSYGIINRTKAQKILKHDGGIL
                     NIKVYDAIFWYYHMVSNQNEVVFEKAIAAMKNAVQIDSNYAMGWAVLSETYVSGVFNG
                     FNSQIENPLQEAIACGKRALRCDPSCQAAYQALALAYLFQHKKKECLDIIEQWQMLKS
                     NAAGIAGGIGFCLICCGKYDAGFKLMQHSIQINPYYPWWFNGGLAFYHYKQKNWSEVV
                     FWSDKLNIYSDSWKLILYIAASFEMGDNIKATAFKLQFSNEFPFILQHLDPYIGAFIQ
                     DKKLSNRLIKVIKLVE"
     gene            complement(1781..2659)
                     /locus_tag="PLO59_02605"
     CDS             complement(1781..2659)
                     /locus_tag="PLO59_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014937280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HQV00018.1"
                     /translation="MKTRSVEMVATPKAPHFVGDGFRVHNFIPSYPMLDMQRMDPFIM
                     LDYNSKYNFPPSEIPKGVGVHPHRGFETVTIAYEGSVEHQDSSGGGGVIGRGDVQWMT
                     AASGVLHKEFHETEWSKTGGVFQMVQLWINLPAKDKMSKPGYQSILNSEIPKVTLADD
                     AGLIEVIAGEYNGSKGSATTFSPLHILNAKLNRNGKADFALPEDFNTALLVIEGSIII
                     NDNEKVPTDNFALMANDGSKFTIEATENAVVLIISGKPLNEPIAAHGPFVMNTHAELV
                     QAFNDFNTGKFGYLED"
     gene            complement(2724..>3000)
                     /locus_tag="PLO59_02610"
     CDS             complement(2724..>3000)
                     /locus_tag="PLO59_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HQV00019.1"
                     /translation="IKKDLIDNLSAKITGFADFNLQLLHKHIASLQKRITQLQSYPAH
                     QRYLDFIQTYPDVLLRVPQTMVASYLGITPESLSRIRKELANSHRTS"
BASE COUNT          929 a          527 c          555 g          989 t
ORIGIN      
        1 atattcaaaa tgtaaaaatt caatggcata tttttactta aggcatatat ctataaactt
       61 ttggttctga attgcgacaa acttaaaatt cttattgttg ccattttaat ataggcagtt
      121 aattaaccgt ataatgaatt tgttaaatca gccaaccgtt gccgaagttt taacacaact
      181 gagccgaata cttgcattta aaacttttaa aaactcaagc attctatcca cctttcttga
      241 ttacattgta agaaccaaac tagcaggaaa tgcaattcag ctgaaggaat atacaatagg
      301 tgtagcagtg ctgttgaaga aagcagatta taaccctcaa actgatgctg ccgtaagaat
      361 tcatgccagt agattgcgta aggcattgga tgaatattac agcatcgaaa cccatgctgt
      421 tgaattattt atatccattc ctaaagggag ctacattcct tattttgaat ggcgaataaa
      481 ttctgataag ggttttgtta atcaaaccga atttgaagga gttaagcctt caattgcatt
      541 gctccctttc catgcttacg atgaaacttt gttgccactt gcagatggta tttgtgatca
      601 gatatgtact gaattgacta attacaatga attgctggtt aaagcttact ttaattcacg
      661 tcatgttgct actcaggtta gtgatattaa aagtgccggg tacttactcg attgtaatta
      721 tcttattacc ggtactattc aaaccgatgg aatgttctta cgttgtcggg ttcaattgat
      781 aagaacgcat gatcagcaac aattatgggc atcggcttat gagaaaaaaa tggctgagtt
      841 taataccttc gaaatacagg atgatattgt tttacatgtt gttaataaca tagctggtag
      901 ttatggtatt ataaatagaa caaaagcgca aaagatatta aaacatgatg gaggaatatt
      961 aaatatcaag gtgtacgatg ccattttctg gtattatcac atggtaagta atcaaaacga
     1021 agttgttttt gaaaaggcta ttgctgcaat gaaaaatgca gttcaaatag attcgaatta
     1081 tgccatgggt tgggctgtac ttagcgaaac ctatgtttcg ggagttttca atggatttaa
     1141 tagtcaaatc gaaaatcctt tacaagaagc aatagcttgc ggtaaacgtg cattacgttg
     1201 tgatccttca tgtcaggcgg catatcaggc tttggcttta gcctatttgt ttcaacataa
     1261 aaaaaaggaa tgcctggata ttattgaaca gtggcaaatg cttaaatcta atgccgctgg
     1321 tattgccggt ggtattggtt tttgccttat ctgttgtggc aagtacgatg caggatttaa
     1381 attaatgcaa cattcaattc agattaatcc ttattaccca tggtggttta acggtgggtt
     1441 ggcattttat cattataaac aaaaaaattg gtcagaagta gtattctggt ccgataagct
     1501 taacatttat tctgatagtt ggaaattaat actttacata gcagcatcat tcgaaatggg
     1561 tgataatatt aaagctacag ctttcaagct acaatttagt aatgaattcc ctttcattct
     1621 gcagcattta gacccctata taggtgcatt tattcaggat aaaaaattat ccaaccgact
     1681 aataaaagta attaaactgg ttgaataaca gttactataa acacaaccag taaatgtagt
     1741 tgctaagttt aaaaaggctt agcaactact tacttcaaaa tcaatcttcc aggtaaccaa
     1801 atttaccggt attaaaatcg ttaaaggctt gtaccaactc agcatgcgta ttcataacaa
     1861 atggaccatg tgctgctatg ggttcattca aaggtttgcc gctgataatt aaaaccacag
     1921 cattttcagt tgcttcgatg gtaaatttac taccatcgtt tgccatgagg gcaaaattat
     1981 cggtaggtac tttttcatta tcattaataa ttattgaccc ttcaattacc aataatgctg
     2041 tattaaaatc ttcaggcagc gcaaaatcag ccttgccgtt tctatttaat ttagcattaa
     2101 gtatatgcaa tgggctaaat gttgtagcgc tacctttact accattgtat tcaccagcta
     2161 ttacttcaat taaccctgca tcatcggcca aagttacctt tggaatttca ctatttaata
     2221 ttgactgata accgggtttg ctcattttat ctttggcggg taggttaatc cacagctgta
     2281 ccatttgaaa aacaccaccg gttttgctcc attcggtttc atgaaattct ttatgcaaaa
     2341 ccccacttgc tgctgtcatc cattgaacat cgcctctgcc aataacgcca cccccaccag
     2401 atgagtcctg atgttcgaca ctgccttcgt atgcaattgt tacggtttca aaacctctat
     2461 gcggatgcac ccctacacct tttggaattt cagatggtgg aaaattatac ttcgaattat
     2521 aatccaacat aataaaagga tccatgcgct gcatatctaa catgggataa cttggaataa
     2581 aattatgtac tctaaagcca tcgcctacaa aatgtggtgc tttaggtgtt gctaccattt
     2641 ctacacttct tgttttcatt gattctgttt ttaaattata gtgcaaaggt gtgccaacaa
     2701 tttgtactgc gaattgatgt atgttaagaa gtgcgatggc tgttggccag ttcttttctg
     2761 atgcggctta agctttcggg ggtgatgcct aaataggagg ccaccatggt ttgtggtacg
     2821 cgcagcaata catcgggata agtttgtata aagtctaaat accgttgatg tgctgggtaa
     2881 ctttgtaatt gtgtaattcg tttttgcagg ctggctatat gcttgtgtaa taattgcaaa
     2941 ttaaaatcgg caaaacctgt aattttagcc gataagttgt ctatcaaatc ttttttgata
//
LOCUS       DAOMAA010000132         2990 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_26374_length_2990_cov_2.595911, whole genome shotgun sequence.
ACCESSION   DAOMAA010000132 DAOMAA010000000
VERSION     DAOMAA010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26374_length_2990_cov_2.595911"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1641)
                     /locus_tag="PLO59_02615"
     CDS             complement(<1..1641)
                     /locus_tag="PLO59_02615"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQV00020.1"
                     /translation="MPKINPDVQISANNLLYVLFTSGSTGMPKGVAMRHEPLVNLALW
                     QKNETTLVDAANTLQFAPISFDVSFQEIISTWSTGGCLFMVDDALRLQATRLLQYIID
                     KNIERIYLPFIALQHLCEVAKQQNIIPYTLKDVITAGEQLQITTQIRSFFTALPDCLL
                     HNHYGPTETHVATAITLEGAPSSWPNLPSIGKAIANDAIYILDENLYPVKVDTEGEIY
                     IAGVGLAQGYLHNDALTAERFLLDALADDPNKRMYKTGDLGKWLPDGNIQYLGRADGQ
                     VKVRGYRIELGEIETTLADFEGITQTAVTVREDKSGLKRLVAYIVCKNFEPNTAAQIR
                     QWLANKLPEYMQPAAYVNLDALPRTPSGKIDRKALPPPHTARTLPEPIVKPESAVEIL
                     IAETWCNLLMLDEVGVNDNFFELGGNSLLALQFIAQYHQRTGTEIPVVKLYQSPTISG
                     LVQLIETPNTLIIQNHPQTPQTDNDIAIVGMSCRLPGANNIDKFWHNLFNGIESITHF
                     NIETISPLVGDVFNDENYVAARGIIDAASFDASFFGMNP"
     gene            complement(1622..2071)
                     /locus_tag="PLO59_02620"
     CDS             complement(1622..2071)
                     /locus_tag="PLO59_02620"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQV00021.1"
                     /translation="MIKNNVFDYHYYQNVLQIIDEQVQLTPNMPAVHHHDKSLSYFEL
                     NQGANALAHLLIDEEVLPNALVGICMDRSLAMAVGILGIIKTGAAYVPFDAEYPTERI
                     QFMLASAQCSVIVTQKKICRFIYQFGCSSFYLGGCIRSAYQHAKDKP"
     gene            2454..>2990
                     /locus_tag="PLO59_02625"
     CDS             2454..>2990
                     /locus_tag="PLO59_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00022.1"
                     /translation="MNKNFWISTACFASILITPLSTQAQTHAITLDVQSTVPSNYFGF
                     NSANLVRPSSPNFDTQWLLDSLESLGTNIVRYPAGTISNYWDWQRGFFDDDMPNGWVL
                     PKDFKDNEYKDMRMDKLKSMVERLGVAPVLNMNLLTKDKYHAAAGVMYAEGLNLNAKY
                     VEFGNELYNDIPLYEERFN"
BASE COUNT          919 a          538 c          576 g          957 t
ORIGIN      
        1 cggattcata ccaaaaaacg aggcatcaaa tgaagctgca tcaataatac cacgagcagc
       61 aacataattt tcatcattaa atacatcgcc taccagaggc gaaatggttt caatattaaa
      121 gtgcgtaata ctttcaatgc cattaaataa attgtgccaa aatttgtcaa tgttgtttgc
      181 accaggcaaa cgacaactca taccaacaat ggcaatatca ttatcggttt gcggtgtttg
      241 aggatgattt tgaataatta gtgtattggg tgtttcaatt aattgtacta atcccgaaat
      301 ggttggcgac tgataaagtt ttaccacggg aatttcagta ccagtacgtt ggtgatattg
      361 ggcgataaac tgcaaggcca atagtgagtt accgcctaat tcaaaaaaat tatcgtttac
      421 gccaacttcg tcaagcatga gcaggttaca ccaggtttcg gcaattaata tttcaacagc
      481 gctttcgggt tttactattg gttctggtaa agtgcgtgct gtatggggtg gtggtagtgc
      541 tttcctgtca attttaccac tgggtgtgcg cggcaaagca tctaaattaa cgtatgctgc
      601 aggctgcata tattctggca atttatttgc cagccattgt cttatctgtg cagcggtatt
      661 tggttcaaag tttttacata cgatgtatgc aactaatctt tttaagcccg acttgtcttc
      721 acgaacggtt acagcggttt gtgtaatacc ttcaaaatcg gccagggtag tttcaatttc
      781 acccagttca attcgataac ctctaacttt tacctgtcca tcagcccgcc ccaagtactg
      841 aatatttcca tcaggcaacc atttacccaa atcgccagtt ttatacatgc gtttattggg
      901 gtcatcagct aatgcatcta gcaaaaaccg ttcggcagtt aaggcatcgt tatgcaaata
      961 gccttgtgcc aaacctactc cggcaatata aatttcgcct tcggtatcaa cctttaccgg
     1021 atataagttt tcatccaaaa tataaattgc atcgttggca attgctttgc caatactggg
     1081 taaattgggc catgaacttg gggctccttc taaagtaatt gcagtggcta catgcgtttc
     1141 tgttgggccg tagtgattat gtaacaaaca atcgggcagt gcagtaaaaa atgaacgtat
     1201 ttgtgtggta atttgtaatt gctcgccagc tgttattaca tcctttaaag tatatggtat
     1261 tatattttgc tgtttagcaa cttcgcatag gtgttgtaag gcaataaatg gtaagtaaat
     1321 acgctcaata tttttatcaa ttatatattg tagtaatcta gttgcctgga gtcttaatgc
     1381 gtcatcaacc ataaataaac acccgccagt gctccaggtg ctaattattt cctgaaacga
     1441 aacatcaaaa ctaatgggtg caaattgtaa ggtattggct gcatctacca atgtggtttc
     1501 atttttttgc cacagtgcta aatttaccaa tggctcgtgt cgcatggcaa ctcccttagg
     1561 catacctgtg ctgcccgaag taaatagtac atacaataaa ttgttggcac ttatttgaac
     1621 atcagggttt atctttggca tgttggtaag ctgacctgat acatcctccc aaataaaaac
     1681 tactgcaccc gaattgataa ataaatcggc aaattttttt ttgtgtaaca ataacgctgc
     1741 attgtgcgga tgctagcata aattgaattc tttctgttgg atattcagca tcaaaaggaa
     1801 cataagctgc tccggttttg ataatgccca aaatacctac agccatagcc agcgacctgt
     1861 ccatacaaat accaaccaag gcatttggta aaacttcctc atcaataagc agatgtgcca
     1921 atgcattagc gccttggttt aactcaaaat aggaaagtga tttatcatga tgatgtacag
     1981 ccggcatatt gggtgtaagc tgcacttgct catcaataat ttgtaaaaca ttttggtaat
     2041 aatggtagtc gaaaacgttg ttcttaatca tcacgggtta catggaagta aagcatgtaa
     2101 aagtaatact aaatgggtgc tgcaaattat gacttcaatc aatgcatagc gttttagtaa
     2161 caatttattg aggataaaat gtgtttacac gtaaaaagta aaatcataaa gtattttact
     2221 ctattggtat taggatggta atttttgtca aattgttgaa aacttttatc attgctaaaa
     2281 ttattcttta acttattgta attcaattat atgtatatta tttagcagat agcttacgtg
     2341 gtaaacaaac attagaaatg ggcgaaatgc atttatttgt tggcgaactg caatttcggt
     2401 attaaattag ctacccaata ttcatattaa ttgttaaaaa ctgcccctca ataatgaata
     2461 aaaacttttg gattagcacc gcttgcttcg ctagtatttt aattacccca ttaagtacac
     2521 aagctcaaac acatgcaatt actttagatg tacaaagtac ggtaccttca aactattttg
     2581 gctttaattc ggcaaactta gtaagaccat catcgcctaa tttcgatacg caatggttgc
     2641 tggatagttt agaatcgctt ggaaccaata tagtaaggta cccggcaggt accatttcaa
     2701 actattggga ttggcaacgt ggtttttttg atgatgatat gcctaatggt tgggtattgc
     2761 ccaaagattt taaggataat gagtataaag atatgcgtat ggataaatta aaatcaatgg
     2821 tagaaaggtt gggcgttgca ccggtgttaa acatgaattt acttactaaa gataagtatc
     2881 atgcagctgc cggtgttatg tatgctgagg gattaaattt aaatgccaaa tatgttgagt
     2941 ttggtaacga attatataat gatattcctt tatatgaaga gcggtttaac
//
LOCUS       DAOMAA010000133         2989 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_26394_length_2989_cov_2.638378, whole genome shotgun sequence.
ACCESSION   DAOMAA010000133 DAOMAA010000000
VERSION     DAOMAA010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26394_length_2989_cov_2.638378"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1574)
                     /gene="dnaG"
                     /locus_tag="PLO59_02630"
     CDS             complement(<1..1574)
                     /gene="dnaG"
                     /locus_tag="PLO59_02630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HQV00023.1"
                     /translation="MILKSSIDKVIEIARIDEVVADYVSLKKRGSNLMGLCPFHNEKS
                     PSFTVSPAKGFYKCFGCGKAGDSIRFVMDYEHVSYPDAIRQLAKKYQIELEETTVTDT
                     EKLAETARENMFTVTAFAQKFYHQYLLDSEAGKAVAQTYFAERGFTDATIKKFQLGFA
                     PEGWQQFTQEALQQQYSLPYLISTGLTIEKEGKHYDRFRDRVIFPIHNVTGRTIGFGA
                     RYLKPQTNAPKYLNSPESDIYNKSKVLYGIHHAKKSIIQNDNCYLCEGYTDVISLSQA
                     GIENVVASSGTSLTVDQIKLISRFTKNITVLYDGDAAGIKASMRGIDLILQEGLNVRV
                     VLFPDGDDPDSFAKRVTTETLQTYLNAEAKDFMLFKTSLLLAENNNDPIGKAKLIRNI
                     VETIAIIPDAIIRSTYTKQCATLMDMAEAILIQEVNSIRRKTVTSQPAVELEIPIEEI
                     IKPETTIQRNRYFEEEEAIRLLLNYAYHILQIATETETVSITVKDFMIAELEQDGIKF
                     ENEIFAQIFANLYIEES"
     gene            complement(1654..2046)
                     /locus_tag="PLO59_02635"
     CDS             complement(1654..2046)
                     /locus_tag="PLO59_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00024.1"
                     /translation="MNALLANILYLMKSNILKTFAVLLVSILSMHLGACKKTSNADAR
                     VTVVDSLNRPIKGAKVVLRQDSVVNPNTGIKADILDEKITDINGNTNHSFKWEAVLNL
                     EVSKGNLKVQDYIRLEQSETVGKTVVLK"
     gene            2157..2771
                     /locus_tag="PLO59_02640"
     CDS             2157..2771
                     /locus_tag="PLO59_02640"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQV00025.1"
                     /translation="MTTTLQAQKPDTDPVVQITTDYGTIKLKLYNETPKHRDNFLKLA
                     KEGFYDGLLFHRVIKDFMIQGGDPNSKNAKPDAMLGSGDVGYTIPAEFNPKFIHKKGA
                     LCAARTENPEKASSGCQFYIVQGKKCTDAELDRYDSNRTVKFTTEQRNAYKTLGGTPF
                     LDMNYTVYGEVISGLDVIDKIAAVKTASADRPATDVKMTVKLVK"
BASE COUNT          893 a          559 c          543 g          994 t
ORIGIN      
        1 gactcttcta tatacaggtt ggcaaaaatc tgcgcaaata tttcgttttc gaactttatt
       61 ccgtcctgtt ctaattcagc aatcataaaa tcttttaccg taatactaac ggtttcggtt
      121 tcggtagcaa tttgtaatat atgatatgca taatttaata ataaccttat ggcctcctcc
      181 tcttcaaagt aacgattacg ttgaatagtt gtttcgggtt taattatttc ttcaatcggt
      241 atttctaatt caacagcggg ttgggaggta accgttttgc gccttatact gtttacctct
      301 tgtattaaaa ttgcttcggc catatccatt aatgttgcac attgtttggt ataagtgctt
      361 ctgataatgg catcgggtat gatggcaatc gtttcaacta tatttctgat aagcttggct
      421 ttgcctatgg gatcgttatt gttttcggct aaaagcaaac ttgttttaaa aagcataaag
      481 tctttggctt cggcatttaa ataggtttgc aaagtttcgg tggttacgcg tttggcaaag
      541 ctatcagggt catcgccatc aggaaacaaa actacccgta catttaatcc ttcttgtaaa
      601 atcaaatcaa tgccccgcat acttgccttt atgccggctg catcgccatc gtataatacg
      661 gtaatgtttt tagtaaaccg gcttataagt tttatctgat ctaccgttaa cgaagtaccg
      721 ctgctggcta ctacattttc aatacctgcc tggctcagtg atattacatc tgtataacct
      781 tcgcataaat aacaattatc gttttggata atactttttt ttgcgtggtg tatgccgtac
      841 aatactttgc ttttgttgta aatatcactt tcgggcgagt tgaggtactt tggtgcattg
      901 gtttgtggtt ttaaataacg tgcgccaaaa ccaatggtac gaccggttac attgtgtatc
      961 ggaaaaatta cacgatcacg aaaacggtca taatgtttgc cttctttttc aatagttaat
     1021 ccggtgctta ttaaatatgg taaactgtat tgttgctgta aagcttcttg ggtaaattgt
     1081 tgccagcctt caggtgcaaa ccccagttga aattttttaa tagtagcatc ggtaaatccg
     1141 cgttcggcaa aataggtttg tgcaactgct ttaccggctt cgctgtcgag taaatattgg
     1201 tggtaaaatt tttgggcaaa tgccgtaacc gtaaacatat tttcgcgtgc ggtttcggct
     1261 aatttttcgg tatcggttac agtggtttct tctaattcaa tctgatattt tttggctagt
     1321 tgtcttattg catccgggta tgaaacatgc tcataatcca ttacaaaacg tatgctgtcg
     1381 cccgctttgc cacaaccaaa acatttataa aaacctttag ctggtgatac tgtaaatgat
     1441 ggcgattttt cgttatgaaa aggacacagg cccattaaat tgctcccccg tttttttaat
     1501 gatacatagt cggctactac ttcgtctatg cgggcaattt caataacttt atcaatagac
     1561 gattttaaaa tcataatgcg caactatggg ctttcattga aaaaaaaatg ctccttttaa
     1621 tgggagcata aatggtatga gtttattgta gttttattta agcaccaccg ttttgcctac
     1681 agtttcgctt tgttctaatc gaatgtaatc ctgcaccttt aaatttcctt tcgatacttc
     1741 taaatttaaa acggcttccc atttaaagga gtgatttgta ttaccgttta tgtcggtaat
     1801 cttttcgtct aaaatatcgg ctttaatacc cgtatttgga tttacaacac tatcctgtct
     1861 taaaaccact ttagcgcctt taataggacg gtttaaacta tctactacag ttacacgggc
     1921 atcggcatta ctggtttttt tacatgcacc caaatgcata cttagtattg ataccagtaa
     1981 cactgcaaaa gtttttaaaa tattcgattt catcagatat aggatatttg ctaataaggc
     2041 attcacttgt aaatcattgc aagtataaat attcttgttt gatttgcgct attaatcgag
     2101 caacttcaaa atatctgcac atgaaaaaac tgctattatt atttacagcc gcaattatga
     2161 caacaacttt acaagcacaa aaacccgata ccgacccggt agttcagata actaccgatt
     2221 atggtaccat caagctaaaa ctgtacaacg aaacacctaa acaccgcgat aattttttaa
     2281 aattagccaa agaaggtttt tatgatggac ttttatttca ccgagtgatt aaggatttta
     2341 tgattcaagg tggcgaccca aactcaaaaa acgccaagcc cgatgcaatg ttaggtagtg
     2401 gcgatgtagg ttataccata cctgctgaat ttaacccaaa gtttattcat aaaaaaggcg
     2461 cactatgtgc tgcacgtacc gaaaaccctg aaaaagcatc gagtggttgc cagttttata
     2521 tcgtacaagg taaaaaatgc accgatgccg agttagaccg ctacgattcg aaccgcactg
     2581 tcaaatttac aaccgagcaa cgtaatgcat acaaaacatt gggaggtaca ccgtttttag
     2641 atatgaatta caccgtttat ggcgaagtaa tttcaggttt agatgtaatt gataaaattg
     2701 ccgcagtaaa aacggcatct gccgacaggc ctgcaaccga tgttaaaatg actgtgaaac
     2761 tggttaaata gttttaagca atcatacata caaagcctgt tgggtatttt tgcccaatgg
     2821 gcttttttat taaacagtaa cgaaaattaa attttaaaaa tgaataccga aaagtttgaa
     2881 aaactgctca atgaaatagg cgattttata ccagaaacca aagaaagtat cgaaagtttc
     2941 agaattaaat ttttgggtaa aaaaggtgtt ttaaccgatt tgtttgcac
//
LOCUS       DAOMAA010000134         2985 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_26459_length_2985_cov_2.490785, whole genome shotgun sequence.
ACCESSION   DAOMAA010000134 DAOMAA010000000
VERSION     DAOMAA010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26459_length_2985_cov_2.490785"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..910
                     /locus_tag="PLO59_02645"
     CDS             <1..910
                     /locus_tag="PLO59_02645"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HQV00026.1"
                     /translation="ALDVIPRLPLLYDEPFADSSAIPTYLVSKMARQHVTVALSADGG
                     DETFAGYTKYAKGLQYINKLNKVPGFVKTLGSLPINITQPFKRSNLAVPDRLEKLKLI
                     LKANHHADAFNVITQAMTVAEVRQLLKPEFTKSITAFDDFANLRSSDMLDHFLWLDYQ
                     TFLVDDILQKVDRATMAVSLEGREPLLDYRIIEWAAQLPAAFKLHNGTGKRILRDITH
                     KYLPKEIMERPKMGFRIPIEKWYKNEHKDILLAYVNADKINAYGLLNHKTIEKMIHAY
                     LKSDIEDFHRIWKILMLQMWMEKWMN"
     gene            complement(911..2164)
                     /locus_tag="PLO59_02650"
     CDS             complement(911..2164)
                     /locus_tag="PLO59_02650"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV00027.1"
                     /translation="MLTTSNAGSAADFMSAVSDANGQVLFYTNGINVWNKKKAIMNNG
                     TGLNGTISSGQSPLIVPQPNSNLYYIFTLDQWANSNGLQISIVDMALQNGFGEVVNKN
                     KPVYSPSTEKITSYYHASENYYWIITHSFSDNEFRCYKLTETGLQSFPTISNSGSVYF
                     GFAGQFNDAVGQLSISSDGTKLVSACYQSGTFEVHDFDINTGIVSNPKLIAGYPNAFG
                     AEFSPDGSKLYVSRWVTSHKVWQYDLNASNWPSVLASVTLVGTGYIVGYLQLAPDGKI
                     YVANYNNNNLGVINSPNSLGLACNYIPFGFSLLPNSSSAGLCATVKKNSLASIKHDNL
                     ISQKINVYPNPSIGNVTIQIENIKNDPLRINIINAKGQTVYNQLKYDSNNTFILEDLP
                     SGIYCLYLTTLLGTNTKQKFIVSNN"
     gene            2393..>2985
                     /locus_tag="PLO59_02655"
     CDS             2393..>2985
                     /locus_tag="PLO59_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00028.1"
                     /translation="MGVIQRQGIKNFISTYVGIVIGFVSLIWIQPNFLTKEELGLTRL
                     LYSFSMMVAMFVPLGIGNATTKYFPLFKNAEKKHYGYFSFMLLFPLLGYTIACIALWF
                     FKDLIFAQYIKESKLFLEFFNYVFPLTLIISLISCLNVYCYVHFKSTIPAFLNDVVSR
                     ILVIVVVTVYYLKWIDFNQFIFCYVALYALQVLLLLA"
BASE COUNT          923 a          515 c          548 g          999 t
ORIGIN      
        1 ggcgctcgat gtgattccgc gtctgccgct gctgtatgac gagccttttg ccgactcatc
       61 ggccatacct acttatttgg taagtaaaat ggcgcggcag catgtaaccg tagccttaag
      121 tgccgatggg ggtgacgaaa cctttgccgg atatacgaag tatgcaaaag gtttacagta
      181 cattaataaa cttaataaag tgcccggttt tgtaaagacg ttaggaagcc tgccaataaa
      241 cataacgcaa ccttttaaac gaagcaacct ggcagtacct gacaggctcg aaaaattaaa
      301 actgatttta aaagcaaatc accatgccga tgcatttaat gtcataacgc aagcgatgac
      361 agttgcagaa gtgaggcaat tattaaaacc cgaatttaca aagagtatta cggcatttga
      421 cgactttgcc aatttgcgca gtagcgatat gctcgaccat tttttgtggc tcgattatca
      481 aacttttttg gttgacgata tactacaaaa ggttgaccgt gccaccatgg ctgtaagttt
      541 agaaggacgc gaacctttat tagattatcg cataattgaa tgggcagcac aactaccggc
      601 tgcatttaaa ctgcataacg gcaccggtaa acgtattttg cgcgatataa cgcataaata
      661 cctgcccaaa gaaattatgg aaagacctaa gatggggttt agaataccta tagagaaatg
      721 gtacaaaaat gagcacaaag acattttgct tgcttacgta aatgctgata aaattaatgc
      781 atacggattg cttaaccata agacaatcga aaaaatgata catgcttatt taaaatcaga
      841 catcgaagat tttcatcgga tttggaaaat tttaatgctt cagatgtgga tggaaaagtg
      901 gatgaattaa ttagttatta gaaactataa atttctgctt agtgtttgtg cctaacaaag
      961 ttgtgaggta taagcaataa atacccgagg gtagatcttc caatatgaat gtgttattgc
     1021 tatcgtactt aagttggttg taaacggttt gacccttagc gtttataata tttatacgta
     1081 aggggtcgtt ttttatattt tcaatctgaa tggtaacgtt tccaattgat gggttagggt
     1141 aaacatttat tttttgacta atcagattgt cgtgtttaat acttgctaat gagtttttct
     1201 ttactgttgc acataagcct gcactacttg aattgggtaa taaactaaat ccaaatggaa
     1261 tataattgca tgcaagcccc aaactattgg gactgttaat aacacccaag ttattattgt
     1321 tgtagttggc cacatatatt tttccatcgg gtgcaagttg taaatagccc actatgtagc
     1381 ctgtaccaac taaagtaaca ctagccagta cactaggcca attacttgcg tttaaatcat
     1441 attgccaaac tttatgactc gttacccatc gtgatacata tagtttcgaa ccatctggtg
     1501 aaaactctgc accaaatgca ttcgggtatc ctgcaatcaa ctttggattg cttacgatac
     1561 ccgtattgat atcaaaatca tgaacttcaa aagtacctga ttggtagcat gcactcacta
     1621 atttagttcc atccgaagag atgctaagtt gtccaactgc atcattaaac tggcctgcaa
     1681 aaccaaagta aaccgaacct gaattactta ttgtaggaaa cgactgtaaa cccgtttctg
     1741 ttaacttgta acaacggaat tcattatctg aaaaactgtg tgttattatc caataataat
     1801 tttcagaggc atgatagtat gatgtaattt tttctgttga aggcgaataa acaggcttgt
     1861 ttttatttac aacctcacca aagccatttt gcaacgccat gtcaacaata cttatttgta
     1921 aaccattgct attagcccat tggtctaaag taaatatata atataaatta gagttgggtt
     1981 gcggtactat tagaggcgat tgacctgacg atatggttcc gttcaacccg gtaccattgt
     2041 tcattattgc ctttttttta ttccatacat taataccatt tgtataaaat aaaacctgtc
     2101 catttgcatc gcttactgca ctcataaaat cagcagcgct acctgcattt gaagttgtaa
     2161 gcactgcatt accactatta aaatcaatac ctgcattatt cccaaagcac cagttattgc
     2221 cttctttttg tgcaaataaa gtggatgtaa ataaacagaa tagaactacc gctattgctt
     2281 gtatttatat actattgaaa gtaaaattga ttttcattag taagagttga tatttataat
     2341 taactaatat tgcctgccat aacacaaatc atcagacaac taacacgtat taatgggtgt
     2401 aatacaaagg caaggtatta aaaattttat ttcgacctat gttggaattg ttattggttt
     2461 tgtaagttta atttggatac agcctaattt tttaaccaag gaagagctgg gccttacacg
     2521 tttgctttat tcattttcga tgatggtggc catgtttgtg cctttaggta taggtaatgc
     2581 aactaccaaa tattttcctt tatttaaaaa tgccgaaaaa aaacattacg gatatttttc
     2641 gtttatgctt ttgtttccgt tgttgggtta tacaatagca tgtattgcac tttggttttt
     2701 taaggactta atttttgcac agtacataaa ggagtcgaaa ttgtttttgg agttctttaa
     2761 ttatgttttt ccgcttaccc taattatttc gctgataagt tgtttaaatg tgtattgtta
     2821 tgtgcatttc aaatcaacca taccggcttt tttaaatgat gtggttagcc gcattttggt
     2881 tattgttgtt gtaacagttt attatttaaa atggattgat tttaatcagt ttattttttg
     2941 ttatgtggct ttgtatgctt tgcaagtatt gcttttgctg gcata
//
LOCUS       DAOMAA010000135         2977 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_26603_length_2977_cov_2.758042, whole genome shotgun sequence.
ACCESSION   DAOMAA010000135 DAOMAA010000000
VERSION     DAOMAA010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26603_length_2977_cov_2.758042"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="PLO59_02660"
     CDS             complement(<1..498)
                     /locus_tag="PLO59_02660"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HQV00029.1"
                     /translation="MHFSCSAQTARPAKKIETQVTRILLLYDDSQSMLAKWETGTRYE
                     VAKRMVSAILDSLKTTPNLELALRVFGHTKRYPPQDCDDTRLEVPFAKNNFDKIKNKL
                     NEIKPSGTTPIARSLEASAGDFSFGNYRNIIILITDGIEECNGDPCAVSAALQKKGII
                     LKPFVI"
     gene            complement(565..1410)
                     /locus_tag="PLO59_02665"
     CDS             complement(565..1410)
                     /locus_tag="PLO59_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013549669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HQV00030.1"
                     /translation="METENLKNICTQTRRDIVRMVHGAQSGHPGGSLGCVEFMVALYN
                     QIMYHQPKPFDMNGIGQDLFFLSNGHISPVWYSVLARSGYFDVAELATFRFINTRLQG
                     HPTTHEHLPGVRVASGSLGQGLSVALGAAQSKKLNGDDKLVYVLMGDGELGEGQVWEA
                     AIYAAAKKVDNIIATVDLNGQQIDGATDEVLPLGNIALKFEAFGWQVLTIKNGGDIAE
                     VIQVLEKAKLQCNQGKPIAIMMHTVMGNGVDFMMHSHKWHGIAPNDAQLEQALQQLPE
                     TIGDY"
     gene            complement(1553..>2977)
                     /locus_tag="PLO59_02670"
     CDS             complement(1553..>2977)
                     /locus_tag="PLO59_02670"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HQV00031.1"
                     /translation="IYGAGQSGVITERTLDRDAGSKYKVLAFVDDDKNKVNRKLEGIE
                     IIDTDGLKDLLKQNHVAHVIISIQNVSAKRKAEIIEACLVHNTSVLNVPPVGNWIKGE
                     LSFNQIKNVKVEDLLEREPIQLSLHDLQQTLSNKVVLVTGAAGSIGSELVRQILKFNP
                     AKILLFDCAESPLHAIELELQPWATICETIVGNIRNQNRVEKVFQTFKPHIVFHAAAY
                     KHVPLMESNASEAIRTNVVGTKIVAHLAHQYNVQTFVLISTDKAVNPTNIMGASKRIA
                     EMYVQSLATVSKTKFITTRFGNVLGSNGSVIPIFKSQIEAGKSITVTHPEITRYFMTI
                     PEACQLVLQAASMGNTGEILVFDMGIPVKIADLAQKMIKLSGLTLGKDIEIVYTGLRP
                     GEKLYEELLNDHENTNATQHKKIMIAKVTPPQFDNIQHQLALLEIKLNEGVNEPLVFL
                     MKQIVPDYKSNNSMYEKLDNKTLE"
BASE COUNT          814 a          592 c          563 g         1008 t
ORIGIN      
        1 aataacaaaa ggttttagaa tgatgccttt cttttgtaaa gctgctgata cagcgcatgg
       61 gtcgccatta cattcttcaa taccatcggt aataagtatg atgatattgc gatagttgcc
      121 aaagctgaaa tcgcctgcac tggcttctaa tgagcgtgca ataggagtag taccgctggg
      181 ctttatttcg tttagtttgt tcttaatttt atcgaaattg ttttttgcaa atggtacttc
      241 taaacgcgta tcgtcacaat cttgcggtgg gtatctttta gtatgaccaa atacacgcaa
      301 tgcaagctcc aggttgggcg tggtttttag gctgtctaat atagccgata ccattctttt
      361 tgctacttca taacgtgtgc cggtttccca cttagccaac atgctttgcg agtcatcgta
      421 taataataat atgcgtgtaa cctgagtttc gatttttttt gcaggccggg cagtttgtgc
      481 agagcatgaa aagtgcaaat tgcttaacaa gcaaaatata aacagcagta acggtttgag
      541 actaaacttc aaaattatac aggtttaata atcgccaatg gtttcgggta gttgttgcag
      601 tgcttgttca agttgggcat cgttaggtgc aataccgtgc cacttgtgtg agtgcatcat
      661 aaaatcaaca ccattgccca taacggtatg catcataata gcaatgggtt tgccttgatt
      721 acattgtagt ttggcttttt ctaaaacctg aataacttcg gcaatatcac ctccgttttt
      781 tatagttaat acttgccagc cgaaagcttc aaacttaagt gcaatgtttc caagtggtaa
      841 tacttcatcg gtagcgccat caatttgctg cccgtttaaa tctactgttg caattatatt
      901 atcaaccttt ttagcggctg cataaatagc agcttcccaa acctgacctt cgcctaactc
      961 gccatcaccc atcagaacat aaactaattt gtcatcgcca ttaagttttt tactttgagc
     1021 agctcccaaa gcaacagata aaccttggcc taatgagcca ctggctacac gtacacccgg
     1081 caaatgttca tgcgtggtag ggtgtccttg taagcgtgta tttataaacc tgaaggtggc
     1141 taactcggct acatcaaagt atccacttcg tgctaacaca ctataccata ccggactaat
     1201 atgtccgttc gacagaaaaa ataaatcctg tccaatacca ttcatatcaa acggtttcgg
     1261 ttgatgatac attatttggt tgtacaacgc taccataaac tcaacacaac ctaatgaacc
     1321 accgggatgt cctgattgtg caccatgtac catacgtaca atgtcgcgtc tggtttgtgt
     1381 acagatgttt ttcaaatttt cggtttccat ataaatgtct gatgcttagc gtttaataat
     1441 ctggcgtata aagcagcata tttgggcttt atacggatgc aaaagtattt tttaattagg
     1501 gcctgtttag ctcaaattat taacattgcg ttgataagtg tttttagaaa agttattcta
     1561 aagttttgtt atctaacttt tcatacatgc tgttattact tttataatca ggtactattt
     1621 gtttcattaa aaaaaccaat ggttcattta ctccttcatt aagttttatt tctaatagcg
     1681 ctaattgatg ttgtatatta tcaaattgcg ggggcgttac tttagcaatc ataatttttt
     1741 tatgctgtgt ggcattggtg ttttcatggt cgttaagtaa ttcttcatac agtttttcgc
     1801 ccggtcttaa accggtgtaa acaatttcaa tatcctttcc taaagtaagt cctgatagtt
     1861 taatcatctt ttgtgctaag tcggctattt ttacggggat gcccatgtca aacactaaaa
     1921 tttcgcccgt attgcccatg cttgctgctt gcaaaaccaa ctgacaggct tcgggtatcg
     1981 tcataaaata gcgtgtaatt tcggggtggg taaccgttat gcttttaccg gcttctattt
     2041 gagatttaaa aataggtata accgagccat tactgcccaa tacattgcca aaacgtgtgg
     2101 taataaattt ggttttgctt acagtagcta acgattgcac atacatttcg gcaatgcgtt
     2161 tactggcgcc cataatattg gttgggttta cagctttgtc ggtactgatg agtacaaaag
     2221 tttgcacatt atattgatgg gctaaatgtg ctactatttt tgtgcctact acattggttc
     2281 tgatggcttc gcttgcattg ctttccatta aaggtacgtg cttgtaagca gcggcatgaa
     2341 aaacgatgtg tggtttaaat gtttgaaaaa ctttttcgac cctgttttga tttctgatat
     2401 tgccaacaat ggtttcgcag atggttgccc atggttgtaa ttctaattca attgcatgca
     2461 ggggcgactc ggcacaatca aacaatagaa ttttggcagg attaaacttg agtatttgac
     2521 gtaccaactc gctgccaata ctaccggctg caccggttac caataccact ttgttcgata
     2581 atgtttgctg taaatcgtgt aagcttaact gaataggctc gcgttccaac aagtcttcaa
     2641 cctttacatt cttaatttgg ttaaagctta actccccctt aatccaatta cctaccggag
     2701 gtacatttaa aacgcttgta ttatgtacca aacaagcttc aataatttcg gccttgcgtt
     2761 tggcgcttac attttgtata gaaataatta catgggctac gtggttttgt tttaataagt
     2821 cttttaagcc atcggtatca attatttcaa tgccttctaa tttacggttt actttgtttt
     2881 tatcatcatc aacaaaagct aaaactttat acttgctacc ggcatcgcgg tctaaggttc
     2941 gttcggtaat aacaccactt tgacccgcac cgtaaat
//
LOCUS       DAOMAA010000136         2966 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_26772_length_2966_cov_3.361044, whole genome shotgun sequence.
ACCESSION   DAOMAA010000136 DAOMAA010000000
VERSION     DAOMAA010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26772_length_2966_cov_3.361044"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1257)
                     /gene="topA"
                     /locus_tag="PLO59_02675"
     CDS             complement(<1..1257)
                     /gene="topA"
                     /locus_tag="PLO59_02675"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008770105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HQV00032.1"
                     /translation="MSENLVIVESPAKAKTIEGFLGKDFTVKSSYGHVRDLAKGDKAI
                     NIEKNFEPQYEISDDKKALIAELQKHVKKASTVWLATDEDREGEAISWHLFETLGLKS
                     KTTKRIVFHEITKGAIQNAIKNPRDIDYKLVDAQQARRILDRLVGFELSPVLWKKVKP
                     SLSAGRVQSVAVRLIVEREREIESHQTTSSFKLVAVFNVTDARGSAFLMKANLQQRFK
                     TETEAVEFLNKCIGAQYSIESLETKPAKKSPAAPFTTSTLQQEASRKLGFSVSQTMRV
                     AQNLYESGKITYMRTDSVNLSHTAIDAAQDAITKLYGSQFVEVRQYKTKNASAQEAHE
                     AIRPTYFENATIEGENAERRLYDLIWKRAIASQMSDAQLEKSIATINISTTSEKFVAT
                     GEILKFEGFLKVYLESKDDEAEEDENN"
     gene            1663..1929
                     /locus_tag="PLO59_02680"
     CDS             1663..1929
                     /locus_tag="PLO59_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007650148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HQV00033.1"
                     /translation="MLTNNDILKKLRVALALKDDDIIAILNLVDFKITKSELGAIFRK
                     DDHPNYMPCGDQLLRNFLNGLVVYKRGPMPTSRPASTENKKTKK"
     gene            complement(1931..2359)
                     /locus_tag="PLO59_02685"
     CDS             complement(1931..2359)
                     /locus_tag="PLO59_02685"
                     /inference="COORDINATES: protein motif:HMM:NF037635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00034.1"
                     /translation="MKSGNQLQNGMRVSMRISDKTPLGYTVLIDDMYEGLLYHNEIFE
                     PIKVGDVRTGFIKLIRADGKIDVALQQQGYLHIEENFSLLLTALQSNNGMLPLGDKST
                     PNDIKQQLGLSKKAFKKMAGYLYKQKIIVITDFEIKLLKP"
     gene            complement(2363..>2966)
                     /locus_tag="PLO59_02690"
     CDS             complement(2363..>2966)
                     /locus_tag="PLO59_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007660462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQV00035.1"
                     /translation="LKGDKVAVISREHAAITRFFEIIMEKEQPYNGAFTWGTTITKAY
                     LPNDNASFFTDDNNLIDWLRQFSKEKDETYIRGFLGKMLFSGEEVLKKSNVLSGGEKV
                     RCMLSRMMMSNANCLIMDEPTNHLDLESITSINNAMKDFKQVALFTSHDHEFTHTVAN
                     RIIEITPNGVIDKRMSYDEYISNEDVAAQKQKLYSVAETA"
BASE COUNT          873 a          566 c          495 g         1032 t
ORIGIN      
        1 attgttttca tcttcttcgg cttcatcgtc tttgctttcg agatagactt ttaaaaaacc
       61 ttcaaatttt aaaatctcac cggttgccac aaatttttcg gaagtagtgc ttatattaat
      121 agttgctata cttttttcaa gttgggcatc actcatttgt gatgcaattg ctcttttcca
      181 gattaaatca taaagtcttc gttcggcatt ttcgccttct atggtggcgt tttcaaaata
      241 agttggccta atggcttcat gcgcttcctg cgctgaagca ttttttgttt tgtattgcct
      301 aacctcaaca aattggctgc catataattt agttatggca tcttgtgcag catcaatagc
      361 agtatgcgat aaatttacgc tatcggtacg catatacgta attttacctg actcatataa
      421 gttttgagct acacgcatag tctgcgatac agaaaaccca agtttgcggc tggcttcttg
      481 ttgcaatgta gaagtggtaa atggtgcagc aggtgatttt ttggcgggtt tagtttctaa
      541 tgattcgata ctgtattgcg caccgataca tttatttaaa aactcaactg cttcagtttc
      601 ggttttaaac cgttgttgca aattagcttt catcagaaaa gcactaccgc gtgcatcggt
      661 aacattaaaa acagcaacta atttaaaaga ggatgttgtt tgatgcgatt cgatttcacg
      721 ttcacgctct acaattaatc gaacggccac gctttgaaca cgacctgccg acaaactggg
      781 ctttactttt ttccataata ccggacttag ttcaaaacct accaacctat ctaaaatacg
      841 cctggcttgt tgtgcatcaa ccaatttata atcaatatca cgtgggtttt ttattgcatt
      901 ttgtatggcg ccctttgtaa tctcatgaaa aacgatgcgt ttggttgttt ttgattttaa
      961 gcctaaggtc tcgaacaaat gccagcttat agcttcgccc tctcggtcct cgtcagttgc
     1021 cagccaaaca gttgaagctt tctttacatg tttttgcaac tctgcgatta aagctttttt
     1081 atcatcgctt atctcatatt gaggttcaaa atttttttca atattaatag ctttatcgcc
     1141 tttggctaaa tcgcgcacat gaccataact tgattttaca gtaaaatctt ttcctaaaaa
     1201 accttcgatg gtttttgcct ttgcaggact ttcaactatc actaaatttt ctgacatcct
     1261 atatttttat aacacaattg aagcaacttt tcgggtggcg ggcaaatata agtgaatata
     1321 aagcggataa tcaaagccgt attttaagaa tattttattc ttgtttttaa gcctaataca
     1381 tttgagtttg cagatgtttt tttttaatca cacataataa agctcctatt gcaacactca
     1441 aattttgtga attttgaaaa gttcaatttt tacaacccag taacttttga atttatagta
     1501 acagttacat tttgactaaa caggcatggg gtaacaacca aaaattatcg tcagataaca
     1561 gtttaaatat tgccatgcct gttattttgt aagtgctata aacattgtat ttaaatgtat
     1621 taaacaatac tattgcataa tactgtttaa acccatttat gtatgttaac taacaacgat
     1681 atattaaaga aactaagagt agctttagcg ctaaaagatg atgacataat tgcaatatta
     1741 aatttggttg attttaaaat taccaaatca gaattgggtg caatattcag aaaagacgac
     1801 caccctaatt atatgccttg tggcgatcag ttgctgcgaa actttttgaa cggattggta
     1861 gtttataaaa gaggccccat gcctacctcc cgccctgcat caacggaaaa taaaaaaaca
     1921 aaaaagtaga ttagggttta agcagtttta tttcgaaatc ggttatcaca attatctttt
     1981 gcttatataa ataaccagcc atttttttaa atgccttttt gcttaagcct aattgttgct
     2041 taatatcatt tggagttgat ttatcgccaa gcggcagcat gccattgttg ctttgcaaag
     2101 cagttaacaa taggctgaaa ttttcttcaa tgtgcaagta tccttgttgt tgcaatgcca
     2161 cgtcaatttt gccatcggca cgaattagtt tgataaaacc tgtacgtaca tcgccaactt
     2221 tgatgggttc aaaaatttca ttgtgataaa gtaatccttc atacatatcg tcaataagta
     2281 ccgtgtatcc taatggtgtt ttatcactta ttcgcatact tacacgcata ccattttgta
     2341 attggttacc agacttcatt gtttaagctg tttcggcaac tgaatataac ttttgttttt
     2401 gagctgcaac atcttcattc gaaatatatt cgtcataact cattcgttta tcaataacac
     2461 cattgggggt aatttcaata attctattgg ctacggtatg tgtaaactca tgatcgtgag
     2521 aagtaaaaag tgccacctgc ttaaagtctt tcattgcatt atttatagat gtaatacttt
     2581 ctaaatcgag gtgattggta ggctcatcca taatcaaaca attggcattg gacatcatca
     2641 tgcgactaag catacagcgc actttttcgc caccgctcag cacattcgat tttttaagaa
     2701 cctcttcgcc actaaacaac atctttccta aaaagccgcg tatataggtt tcgtcttttt
     2761 ctttcgaaaa ctgcctgagc caatctatca aattgttatc gtctgtaaaa aaagaagcat
     2821 tatcattggg taaataagct tttgttatgg tagtacccca ggtaaaggca ccattgtaag
     2881 gctgctcctt ttccataatg atttcgaaaa aacgtgtaat tgcagcatgt tcgcgcgaaa
     2941 tgactgccac cttatcgcct tttaaa
//
LOCUS       DAOMAA010000137         2964 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_26813_length_2964_cov_2.802681, whole genome shotgun sequence.
ACCESSION   DAOMAA010000137 DAOMAA010000000
VERSION     DAOMAA010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26813_length_2964_cov_2.802681"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="PLO59_02695"
     CDS             complement(<1..252)
                     /locus_tag="PLO59_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019986882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HQV00036.1"
                     /translation="MSHIVAPSILSADFGNLQADCEFVNASAADWFHVDIMDGVFVPN
                     ISFGFPVLKAIQKHATKPLDVHLMIVEPDKFVQQFAQHGA"
     gene            complement(297..995)
                     /gene="mtgA"
                     /locus_tag="PLO59_02700"
     CDS             complement(297..995)
                     /gene="mtgA"
                     /locus_tag="PLO59_02700"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HQV00037.1"
                     /translation="MLKILVLFLWKWIKRLALFFIISTVVAVILYKWIPIPYTPLMII
                     RCFEQKAAGKPIKLEKDWVPLSQISARLPEAVIASEDQKFEEHFGFDIEAIEKAQQYN
                     ARMKGKRVRGASTITQQTAKNVFLTQSRTYWRKALEVYFSFLIEVCWSKERIMEVYLN
                     VIEMGDGIYGAEAAAQAYFNKIALKLTAKEASLIAACLPNPRIYIANKPSRYIERKSV
                     WIMRAMRRLDYKNF"
     gene            complement(997..1908)
                     /locus_tag="PLO59_02705"
     CDS             complement(997..1908)
                     /locus_tag="PLO59_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00038.1"
                     /translation="MLKAIVKPFYKTAHDLLYATAYYNPLFKKQHHNKKVTKGFIGDE
                     HLQNPILDDELFKELQEQKIATSTYQISVDGYNAYMKQAGYPVTYYGGGKDLKQNFIQ
                     KTLEHYVSQTLFTLTVNDVFLDVAACNSPFSQIIQNQTGAKVYQQDLIYKPGINGKYI
                     GGYGHQTNLPANSIKAITLHCSLEHFEHDTDIQFFKEVNRILILGGKAIILPFYFAHT
                     YTIHIDPAFNLLRNHHPIIDNEADLRYCNWYQFHSRHYDINQLQKRIVASLPQCKVKL
                     HRVSNFTAIQPAGYLRWILEITKEATN"
     gene            complement(1917..>2964)
                     /locus_tag="PLO59_02710"
     CDS             complement(1917..>2964)
                     /locus_tag="PLO59_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HQV00039.1"
                     /translation="NIDNVTIPEDLKQVALFLINHPNIASKRWVYTQYDSMVGTVNRS
                     TNTCTDAAIVNLKDSKKALALTVDCNSRYVFANPETGCAIAVAEAARNITCSGGQPSA
                     ITNCLNFGNPYDPEAYWQFVHAIKGMGKACLKFNTPVTGGNVSFYNQSEVENHTVPVF
                     PTPTIGMLGILADKNRTMTYNFKQAGHHIYLLGSSQNCISSSQYLAHFHQIKFSPAPY
                     FSLDEEYDLHQLIKQLINADLIQSAHDCSEGGLWVALAESGFNNQLGFNIITDADIRK
                     DAFLFGEAQGRVVVSISPEQFDLFIETVVASGIDFSHLGDVTTGEIKIDGNYWTSIVD
                     LQNQYDTAIEKALL"
BASE COUNT          884 a          549 c          542 g          989 t
ORIGIN      
        1 tgcaccatgt tgggcaaact gttgcacaaa tttatcaggc tcaacaatca ttaagtgcac
       61 atctaacggt ttggttgcat gcttttgtat ggcttttaat accggaaaac caaacgatat
      121 attgggaaca aaaacaccat ccataatatc tacatgaaac caatcggccg ctgaggcatt
      181 aacaaattcg caatcggcct ggaggttacc aaaatcggcc gaaagaatgc tgggtgcaac
      241 tatatgactc atttatattg tatttaataa tttaaaacga atgcaattta tttaaattaa
      301 aaatttttgt aatctaatct gcgcatagca cgcattatcc aaaccgattt acgttcaata
      361 tatctcgatg gtttatttgc aatataaatg cggggattgg gtaagcaggc tgctattaac
      421 gatgcttctt tagcagtaag ctttaatgca attttattaa aatatgcctg tgcagccgct
      481 tcggcaccat atataccatc acccatttca atcacattta aatatacttc cataatgcgc
      541 tctttgctcc agcatacttc aattaaaaac gagaagtaaa cctccagtgc tttacgccaa
      601 taggtgcgcg attgcgttag aaaaacattt tttgctgttt gttgtgttat cgtactggca
      661 cctcttacac gtttgccttt catgcgtgca ttgtattgtt gcgctttttc aattgcttcg
      721 atatcaaagc caaagtgttc ttcaaatttt tggtcttcgc tggctattac ggcttcgggc
      781 aaccgtgctg atatttgcga aagtggaacc cagtcttttt caagtttaat tggtttaccc
      841 gctgcttttt gctcaaagca tctgattatc attaatggcg tgtagggtat gggtatccac
      901 ttataaagta ttaccgcaac tacagtgctt ataataaaaa acaaagccaa acgcttaatc
      961 catttccata aaaaaagtac aagtatttta agcattttaa ttagttgctt cctttgttat
     1021 ttctagtatc catcgtaaat aaccagcggg ttgtattgct gtaaaattgc taacgcgatg
     1081 tagttttact ttgcattgag gtaagcttgc tacaattcgt ttttgtaatt gattaatatc
     1141 ataatgcctg ctatgaaatt gataccagtt acaatagcgc aaatcggcct cgttatcaat
     1201 tattgggtgg tggtttcgta acaaattaaa tgctgggtca atgtgtatgg tgtaagtatg
     1261 tgcaaaataa aatggaagta taatggcttt gccacccagt attaaaatcc ggtttacctc
     1321 tttaaaaaac tgaatgtcgg tatcgtgctc aaaatgttct aacgaacagt gcagggtaat
     1381 agccttaatg ctatttgccg gcaggttggt ttgatggcca taaccaccaa tgtacttgcc
     1441 gtttatgccg ggtttataaa ttaaatcctg ctgatatact ttagctccgg tttggttttg
     1501 tataatttgc gaaaatggcg aattgcaagc tgcaacatct aaaaaaacat catttacagt
     1561 aagtgtaaat aatgtttgtg atacatagtg ctctaaagtt ttttgaataa aattttgctt
     1621 taagtctttt ccgcctccgt aataggtcac cggataaccg gcttgcttca tataggcatt
     1681 atatccatca acagaaattt gataagttga agttgcaatt ttttgctctt gcaattcttt
     1741 aaacagttca tcatccaaaa tcggattttg taaatgttca tcaccaataa aaccttttgt
     1801 cactttttta ttatgatgtt gttttttaaa aagcggattg tagtatgcag ttgcatacaa
     1861 taaatcgtgt gctgttttat aaaatggttt tactatggcc tttaacatct ttgtacttac
     1921 aacaaggctt tttcaatggc tgtatcgtat tggttttgta aatcaacaat tgaagtccaa
     1981 tagttgccat caattttaat ttcgcctgtt gtaacgtcac ctaagtggct aaaatcaatg
     2041 ccacttgcaa caactgtttc tataaacaaa tcaaattgtt cagggcttat cgaaactact
     2101 accctaccct gtgcttcgcc aaacaaaaat gcatctttac ggatatctgc atcagtaata
     2161 atgttaaatc ctaattgatt attaaagccc gattcggcta aggcaaccca caaaccacct
     2221 tctgagcaat cgtgtgccga ttgaattaaa tcggcattta taagttgctt aattagttga
     2281 tgtaagtcat actcttcatc taaactaaaa taaggggcag gcgaaaattt tatttgatga
     2341 aaatgtgcca gatattgcga tgagctgata caattttgtg aagaacctag caaataaata
     2401 tgatgacctg cttgcttaaa attataggtc attgtgcggt ttttatcagc taaaattccc
     2461 aacatgccaa tggttggcgt aggaaaaacc ggtacggtat ggttttcgac ttcgctttga
     2521 ttgtaaaaac taacattacc accggtaacg ggtgtattaa actttaaaca agctttaccc
     2581 attcctttaa ttgcatgtac aaattgccaa taagcttcgg ggtcgtaggg gttaccaaag
     2641 tttaagcaat tggtaatagc actgggttgg ccaccactgc aggttatatt acgggctgct
     2701 tcggcaacgg caattgcaca acccgtttcg ggattcgcaa atacataacg tgagttacag
     2761 tcaacggtta atgccaacgc ttttttcgaa tcttttaaat taacaatggc agcatcggta
     2821 caggtattgg tacttctgtt aacggtacca accatgctat cgtactgcgt atatacccat
     2881 cgtttgctgg ctatgtttgg atgattgatt aaaaataatg ctacttgttt taaatcttca
     2941 ggtatggtta cattatcaat attg
//
LOCUS       DAOMAA010000138         2945 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_27129_length_2945_cov_2.683737, whole genome shotgun sequence.
ACCESSION   DAOMAA010000138 DAOMAA010000000
VERSION     DAOMAA010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27129_length_2945_cov_2.683737"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="PLO59_02715"
     CDS             <1..560
                     /locus_tag="PLO59_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HQV00040.1"
                     /translation="RVALVGYTNVGKSTIMNALSKSAVFAENKLFATLDTTVRKVVLD
                     QIPFLLSDTVGFIRKLPVDLVESFKSTLDEVREADLLIHVVDISQPHFEAHLQVVNQT
                     LVDIGAANKPLLLVFNKMDAYQSTYDDETAETWVDFEKSWMAKINAPCVFISATEKQN
                     MQQFKATLIEMVKTLHLQRYPNISK"
     gene            663..1226
                     /locus_tag="PLO59_02720"
     CDS             663..1226
                     /locus_tag="PLO59_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00041.1"
                     /translation="MQNLKLLAIAIIAFIVGFFACKNFFCKQRDDKAQIPQYFPVKQI
                     FNDYQTGMARNSNFAGLEFTKEDLTELANSSLTDTVQMIIAIDKGRNKVVYFRGVYDT
                     AKPSGEDTVYYFANNQMQKITPTQLTIQFYAQSHSLLKDVFESVFTTAAAAKLPRCPP
                     YNDPRCSAVQAATLDPINGSGEVEPIL"
     gene            1381..>2945
                     /locus_tag="PLO59_02725"
     CDS             1381..>2945
                     /locus_tag="PLO59_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00042.1"
                     /translation="MPYINTIHQSADGYIWLGGDEGLQQFDGNRFYNFNQIEDPKYKP
                     SDKRIWKLLSDKKNNLWMCTQSGVYYYNSQTHLVTNCHLPDSQPFFSMFAAINLQGNA
                     MFATYRNGLLVSDTNNNLRPITHVLNDTNHLTSQMVALHQTTNGLWLATAKGKFYLLR
                     QANDTAVKSLDVVWVDKPKHVFILHVVYNSLAKTWMLSVALDSLVTKTAIYEAKFSDN
                     KVFIQQLKLPKSCNYVVKHLAFDNAHNLWLCFVNRLPVKLTLNVSNGNSTIIHDDPLL
                     CYNSEEVKITNVNATYCDLYNRLWCISDDKIFMTSLNNQNTQAPIGIKSMPDINAYNH
                     ADALHQTDSGIYLTMPGDTIIYAGFNGTSKKISFRYNDGLSIFSLKTDYNNDMYLATF
                     NGVKHIPQKYISDKFNIVDTTAFKNNPIQALIDNVINTKMSTCIYKAADSSLFFGCFN
                     GLYKLNKNRTHISTIKYNKANLPTVEMVNFNNAYYVAAEAGVYTLNFNDTVAQPCTNN
                     ALAALRNQQVACLT"
BASE COUNT          982 a          538 c          528 g          897 t
ORIGIN      
        1 tgcgtgtagc tttggtgggc tataccaatg ttggtaaatc aaccattatg aatgcattaa
       61 gcaagagcgc tgtttttgcc gaaaataaat tgtttgcaac gctcgataca acggtgcgta
      121 aagtggtgct cgatcaaata ccttttttgt taagcgatac cgtaggcttt atacgcaagt
      181 tgcctgttga tttggtcgaa tcgtttaaat caaccttaga cgaagtacgc gaagccgatt
      241 tactcattca cgtagtagat atttcgcaac ctcatttcga agcgcactta caggtagtta
      301 atcaaacgtt ggtagatata ggggcagcca acaagccctt acttttggtt tttaataaaa
      361 tggatgcata ccaaagcact tatgatgacg aaacagccga aacctgggtg gatttcgaaa
      421 aatcgtggat ggcaaaaata aatgcaccct gtgtttttat atcggcaacc gaaaaacaaa
      481 atatgcaaca gtttaaagcc accctaatcg aaatggtaaa aacattgcat ctacagcgtt
      541 atccaaatat tagtaaataa gtcttgcatt tttaatttgc atacttatat ttggcctgat
      601 gacaataaaa tttattgtat caaaaagtta ctaattaatt aaccatttaa acccacctac
      661 ttatgcaaaa cttaaaatta ttggctattg ccatcatcgc atttattgtt ggtttctttg
      721 cttgcaaaaa ctttttttgc aagcaaagag acgataaagc ccagattcca cagtattttc
      781 cggtaaaaca aatttttaac gattatcaaa caggtatggc tcgtaattcg aattttgccg
      841 gccttgaatt tacaaaagaa gacttaactg aattggcaaa cagcagtcta accgatacag
      901 ttcaaatgat tattgccatt gataaaggta gaaataaggt agtatatttt agaggtgtat
      961 atgatactgc taaaccatca ggtgaggata ccgtttatta tttcgccaat aaccaaatgc
     1021 aaaaaataac cccaacgcag ttaaccattc aattctatgc gcaaagccat tcgttgctaa
     1081 aagatgtttt tgaaagtgtg ttcacaactg ccgctgcagc caaactacct aggtgcccac
     1141 cctataacga ccctcgttgt tcggcagtgc aagctgccac attagatcct ataaatggta
     1201 gtggcgaagt tgaaccgata ttgtaataac ggacagcaat atgatatcga accggagtag
     1261 ttatttactc cggttttttt tattattacc tgcatgaatc gattgttaat acttttcctg
     1321 atgcttctgt tttgcagcct tttgcacggc cagcaatgct acaaatttaa gcagtataat
     1381 ttgccctaca tcaataccat tcatcaatcg gccgatggtt atatttggtt gggaggtgat
     1441 gagggcttac agcagtttga cggcaatagg ttttataact tcaaccaaat tgaagatcct
     1501 aaatacaagc catcggataa gagaatctgg aaactgctat ctgataaaaa aaataactta
     1561 tggatgtgca cgcaaagcgg agtttattat tacaactcac aaacccactt agtaactaat
     1621 tgccatctgc ccgattcgca accgtttttc agcatgtttg cggccattaa tttacaaggt
     1681 aatgccatgt ttgctaccta tagaaatgga ttgctggtaa gcgatacaaa taataaccta
     1741 aggccgataa cacatgtgct aaacgataca aaccatttaa cttcacaaat ggttgcatta
     1801 caccaaacta caaatggatt atggttagct acggcaaagg gtaagtttta tttgttacgt
     1861 caagccaacg ataccgctgt aaaaagttta gatgttgttt gggtcgataa gcctaaacac
     1921 gtatttattc tgcatgtcgt ttataatagc ttggcaaaaa catggatgtt gtcagtagca
     1981 ttggacagcc tggttaccaa aacagccatt tatgaagcta aattttctga caacaaagtg
     2041 tttatccaac agttaaaact gccaaagtct tgtaactatg ttgtaaagca tttagcgttt
     2101 gataatgcac ataatttatg gctatgcttt gtaaaccgat tgcctgttaa gcttactcta
     2161 aatgtaagca acggcaattc aacaattata catgatgacc cattgttgtg ttataattcg
     2221 gaagaagtaa aaattaccaa tgtaaatgca acatactgcg atttgtataa caggctttgg
     2281 tgtattagcg atgataaaat ttttatgaca tcattaaata atcaaaatac acaggcgccc
     2341 ataggtataa aatcaatgcc tgatattaat gcatacaatc atgctgatgc attacatcaa
     2401 accgatagtg gtatttacct aaccatgccc ggtgatacta taatttatgc aggttttaac
     2461 ggaactagta aaaaaattag tttcaggtat aatgacggct taagcatatt tagtttaaaa
     2521 accgattata ataatgatat gtatctggca acttttaatg gcgttaagca tattccgcaa
     2581 aaatacattt cagataaatt caacattgta gatactacgg cttttaaaaa caatcccatt
     2641 caggcactaa tagataatgt tattaatact aaaatgagca cctgtattta taaggcagcc
     2701 gacagcagtt tattttttgg atgctttaac gggctttata aacttaataa gaatcggacc
     2761 catataagta ctataaaata taacaaggct aatttgccaa cagttgaaat ggttaacttc
     2821 aataatgcat actacgttgc agcagaggcc ggtgtttaca cattaaattt taatgatacc
     2881 gttgctcaac cttgtacaaa taatgcatta gcagctttgc gaaaccaaca agttgcgtgt
     2941 ttaac
//
LOCUS       DAOMAA010000139         2932 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_27353_length_2932_cov_3.154327, whole genome shotgun sequence.
ACCESSION   DAOMAA010000139 DAOMAA010000000
VERSION     DAOMAA010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27353_length_2932_cov_3.154327"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1326)
                     /locus_tag="PLO59_02730"
     CDS             complement(<1..1326)
                     /locus_tag="PLO59_02730"
                     /inference="COORDINATES: protein motif:HMM:NF019912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00043.1"
                     /translation="MKAWLFRLSFLITYSAFAQFNVALTQQAVHLFPSLPVKSSYTSL
                     MDRRSGYDYLYSANMESGLGIYILLGGNNITPVLNLSGAIFNNLDAATIEQKGNSLFV
                     GIGDFQVNNNAASGLAILDITNPASPFIKDIWDSTVFKHGISHILLNGNFAYLSTMTN
                     GIIILDVSDENNIVFKSKLQLDLAFPSPSINAHNARGLKIKNDTLYVCFDRGGLRIVD
                     VVNKSTPVEVYKYINTSLTSQAAAAYNDVAIKGNYAFISVDYCGLEILDISNIPYTTV
                     QWYNPNGCSPTNWSGAAIHTNEVMLANNDSLLFVSGGQSEVLVFDVTNPLSVTKVGEF
                     VNLNDTLATHGLDVYNNQVILSLIHTPIHIPPFTPFYADPGGLKLLNYIVSTTASANT
                     TVTNNTVNIYQLQNNGVVIESPTQIDAITIYNIYVKAVFTATAIHLNKYH"
     gene            complement(1404..2681)
                     /locus_tag="PLO59_02735"
     CDS             complement(1404..2681)
                     /locus_tag="PLO59_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HQV00044.1"
                     /translation="MNEIICPNCHKAFKVDQAGFAEIVKQVRNQQFDAELSQRLALAE
                     KEKESAVKLAEAHIKQSLQAQLALKDNELTTLKSQSALTLSEQLNKKDTELNQLKAKI
                     ENSETEKKLAVTQATQIIEKERDVLANDLRTKELEKQNLASALKQQFASELQNKEAII
                     KYKDEEIARVKDMKARQSTKMHGESLEIHCETEFNKLRATAFQKSYFEKDNDVKTGSK
                     GDYIFKETDEAGVEIISIMFEMKNEGEETATKKRNEDFFKELDKDRNEKKCEYAVLVS
                     MLESESDLYNSGIVDVSYKYEKMYVIRPQFFIPIITILRNAAMHSLKAKTELALVKSQ
                     NIDITKFEDNMNAFKAAFAKNYEHASKQFKTAIEEIDKTIDHLNKTKEALLLSVNHLR
                     SANNKAEDLTIKKLTRGNPTMTAKFEDLKNDTE"
     gene            complement(2752..>2932)
                     /locus_tag="PLO59_02740"
     CDS             complement(2752..>2932)
                     /locus_tag="PLO59_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00045.1"
                     /translation="IYLKADAKLIIRNGSSLWLNDAQIIAEKGSRITIYKKQNYHTSG
                     DGYIHTQNKRQLFFK"
BASE COUNT          805 a          508 c          535 g         1084 t
ORIGIN      
        1 atggtattta ttcaaatgta tggctgtagc tgtaaaaact gctttgacat aaatattgta
       61 aatagttata gcatcaattt gtgttgggct ttcaattaca acaccgttgt tttgcagttg
      121 atatatatta actgtattgt ttgttacagt agtatttgcc gatgctgtag ttgaaacgat
      181 atagtttaat aattttagtc cacccgggtc ggcataaaaa ggtgtaaatg gtggtatgtg
      241 aatgggtgta tgtattaagc ttaatattac ctggttgtta taaacatcta aaccatgtgt
      301 tgccaacgta tcattaaggt ttacaaattc gccaactttg gttacactta gtggattggt
      361 tacatcaaaa acaagcactt ccgactggcc accacttaca aaaagtaaac tatcattatt
      421 agccagcatt acctcattgg tatgtatggc cgcgccactc caattggtag ggctgcaacc
      481 attggggtta taccattgca ccgtagtgta tggtatattg ctgatgtcta aaatttcaag
      541 gccgcaataa tcaaccgata taaatgcata gttgccttta atagccacat cattgtatgc
      601 cgcagcagcc tggctggtta aactggtgtt gatgtattta taaacttcaa ccggtgttga
      661 tttattgaca acatcaacaa tgcgcaggcc acccctgtcg aaacatacat aaagtgtatc
      721 gtttttaatt tttaagccgc gtgcattatg tgcattgatt gatggtgatg gaaaagctag
      781 gtctaattgt aatttagatt taaatacaat gttgttttcg tctgaaacat ctaaaatgat
      841 gataccatta gtcatggttg acaggtatgc aaaattgcca tttaaaagta tatgcgaaat
      901 gccatgttta aataccgttg aatcccaaat gtcttttata aaaggagatg ccgggtttgt
      961 aatatctaaa atagctaaac ccgaagctgc attgttgtta acctgaaaat cgccaatgcc
     1021 tacaaaaagc gaattgccct tttgttcgat agtagcggca tctaaattat tgaaaatggc
     1081 gcccgataag tttaacaccg gagtaatgtt attgccgccc aaaagtatgt agatacccaa
     1141 acccgattcc atattggctg aatacaaata atcgtatccc gaccgtctgt ccattaacga
     1201 agtgtaggat gatttaaccg gcaggcttgg aaacaaatgt accgcttgtt gtgtaagtgc
     1261 tacattaaac tgtgcaaaag ccgaataggt aatcagaaaa ctaagacgaa acagccatgc
     1321 tttcatctgc aataaatttt agcaacattt aaatgtagca caattattaa acagacatga
     1381 tgagatttag attaaagcaa aacttattcg gtatcgtttt tcaagtcttc aaattttgca
     1441 gtcatggtag gattgccacg tgttaatttt tttatggtca ggtcttcggc tttgttgttg
     1501 gccgagcgta aatgatttac tgataaaagc aaagcctctt ttgttttatt taaatggtca
     1561 atggttttat caatttcttc aattgctgtt ttaaactgtt tcgatgcatg ctcgtagttt
     1621 tttgcaaaag cagctttaaa ggcattcata ttatcttcga acttagttat gtcaatgttc
     1681 tgacttttaa ccaatgccag ttcggtttta gcttttaacg aatgcatggc agcattgcgc
     1741 agtatggtaa taatggggat aaaaaactgt gggcgtatca catacatttt ttcgtattta
     1801 tacgatacat ccacaatacc tgaattgtat aaatcgcttt cgctttcgag cattgaaact
     1861 aaaaccgcat actcacattt tttttcattc ctgtctttat ctaactcttt aaaaaaatct
     1921 tcgtttcttt tttttgttgc agtttcttcg ccttcatttt tcatttcaaa cattatcgaa
     1981 attatttcaa caccagcctc atcggtttct ttaaaaatgt aatcgccttt gctgcctgtt
     2041 tttacatcat tatctttttc gaaataagat ttttgaaatg ctgtagcacg taatttatta
     2101 aactcggttt cgcaatgtat ctcaagcgat tcgccatgca ttttggttga ttgtcgggct
     2161 ttcatatctt ttactctggc tatctcttcg tctttgtatt taataatggc ctctttgttt
     2221 tgcagttccg atgcaaattg ttgttttaat gcgcttgcca gattttgttt ttcaagctct
     2281 ttagttctta aatcatttgc caaaacatcg cgctcttttt caattatttg tgttgcctgt
     2341 gtaacggcaa gttttttctc ggtttcgcta ttttcaattt tagcttttaa ctgattgagt
     2401 tctgtatcct ttttatttaa ttgttcggat agcgttaatg cactttgcga tttaagcgtt
     2461 gttagctcat tatcttttag tgctagttgt gcttgtaaag attgcttaat atgtgcttcg
     2521 gctaatttta ctgcactttc tttttctttt tcggcaagtg ccaaacgttg gcttagctct
     2581 gcatcaaact gttggttgcg tacctgtttt actatttcgg caaagcctgc ctgatctact
     2641 ttaaaggctt tatggcagtt ggggcatatt atttcattca tggtttttaa cttgtatatt
     2701 tttatttgaa atcggttgtc ggattattgt gttactaata aatgattgag tttatttaaa
     2761 aaacaattgt cgtttgtttt gcgtatgtat gtagccatca ccgcttgtat gataattttg
     2821 ttttttataa atggttatgc ggctaccttt ttcagcaata atttgtgcat cgttaagcca
     2881 tagggagctc ccatttcgaa tgataagttt tgcatcggct tttaaatata tt
//
LOCUS       DAOMAA010000140         2925 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_27467_length_2925_cov_2.483624, whole genome shotgun sequence.
ACCESSION   DAOMAA010000140 DAOMAA010000000
VERSION     DAOMAA010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27467_length_2925_cov_2.483624"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            258..1502
                     /locus_tag="PLO59_02745"
     CDS             258..1502
                     /locus_tag="PLO59_02745"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQV00046.1"
                     /translation="MQATAQNHTTLTANQKSILFTQATIHNGRGEVLTAAALGFKNGK
                     ITYIGTDTKLPVNDYDTVINCGSKHIYPGFIGMNTQMGLVEIEAVRATIDAGEVGDIN
                     PSARSVIAYNTDNKSIATVRCDGVLMCQVVPQRGLVSGQSSVMKMDGWNWEDAAYKID
                     EGIIIHWPRMMLFNAWWASSPEEQQQRTERQLLQLKNLFDEARAYYMSKGSGNINLDA
                     MCGLFNHSKKLYVEAYSVREIIAAVAFGNSYGVSLVIVGGIESCMVADLLKKHNIPVI
                     IKGTHTLPATDDTDIRATYKLPALLQQAGITYCISVEGFWQVRTLGYQAGQAVAYGIT
                     AEEALTAITYTPAKILGIDASCGSLEKGKDATLFISEGDALDMRTNKISWAFIAGKPV
                     DLDNVQKQLYRKYLDKFGLHDN"
     gene            complement(1499..2761)
                     /gene="rodA"
                     /locus_tag="PLO59_02750"
     CDS             complement(1499..2761)
                     /gene="rodA"
                     /locus_tag="PLO59_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014200470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HQV00047.1"
                     /translation="MRQQKSIFDNIDWPTVSIYIVMVLFGWLNIFSAVYNEAHPSILD
                     ATQNYGKQLMWIAVSALFAIIILIIDGKFYEAFAIPFYVLMIALNIGAIFLSRDVKGA
                     HAWIPLGSFNLQPSEFAKLATALALAKYLSSPYIRIAENKTKIIASFMILLPMAIILL
                     QNDTGSALVFASFLFVLYREGLSQNVLIFGFIAALLFVLSLMVAKVYLIIAIVVVLVL
                     TWFTLRRTWRNFLALTVVGCLAIGLVYSVDYVYNTALQPHQKQRIDVLLGKKTDLKGA
                     GYNVNQSLIAIGSGSITGKGFLQGTQTKFDFVPEQSTDFIFCTVGEEWGFIGTSSVLL
                     LFTVLLYRIIIIAERQKNAFVRVYGYGVASILFFHVFINVGMAIGLLPVIGIPFPFFS
                     YGGSSLISFTLLLFILIKLDSYRMYVLR"
BASE COUNT          976 a          566 c          566 g          817 t
ORIGIN      
        1 gacaaaccca atgacgttat tggcattgtc atagctaatt ttgccggctg cggggtcaaa
       61 atccaatact ggatcgatga tggcacagtg tttgctagct gtatcgatta gaacgtgggt
      121 aaaagtttca gtgtctttgt ctaaaaaaga atgaatattc ataagttttc ctgttctcta
      181 aaaacgcttt tggcatatct ttatggcaat ttcaactgaa aaaacacttt atactttggc
      241 tgttaactgt tgcttgtatg caagcaacag cacaaaacca cacaacgctt acggcaaacc
      301 aaaaatcaat tttatttacc caagcaacca ttcacaatgg ccgtggtgag gtattaacgg
      361 ctgctgcatt gggttttaaa aacggcaaaa ttacctatat aggaacagat acaaaattac
      421 ccgtaaacga ttacgataca gtaataaatt gtggcagcaa acatatttac cccggcttta
      481 ttggcatgaa cacgcaaatg ggtttggttg aaattgaagc cgtacgcgct acaattgatg
      541 caggcgaagt tggcgacatt aaccccagtg cccgcagtgt tattgcctat aataccgata
      601 ataaaagtat tgcaaccgtg cgctgcgatg gcgttttaat gtgccaggtt gtaccacaaa
      661 ggggcttggt ttcgggacaa tcaagtgtaa tgaaaatgga tggatggaat tgggaagatg
      721 cagcttataa aatagacgaa ggcattatca tacactggcc tcgtatgatg ctgtttaacg
      781 cctggtgggc ttcatcaccc gaggagcaac agcaacgtac ggagcgccag ttgttgcagt
      841 taaaaaattt gtttgatgaa gcgcgcgctt attatatgtc gaaaggaagc ggcaatataa
      901 atttagatgc catgtgtggt ttgtttaatc attcaaaaaa gctttatgtt gaagcatatt
      961 cagtacgcga aataattgcc gctgtagcgt ttggtaattc gtatggtgta tccttggtaa
     1021 tagttggagg cattgaaagt tgcatggtag ccgatttgtt aaaaaaacat aacatacctg
     1081 ttattattaa aggcacacat acgttgcctg ctactgacga tacagacata cgtgctacct
     1141 ataaattacc cgcattgtta cagcaagccg gtattaccta ttgtataagt gttgaagggt
     1201 tttggcaagt acgtacctta ggttaccagg cagggcaagc ggttgcttat ggcataactg
     1261 ccgaagaagc attaacagcc attacctaca cccctgctaa aattttaggt attgacgcaa
     1321 gttgtggtag tttggaaaaa ggtaaagatg caaccttgtt tatttccgaa ggcgatgctt
     1381 tggatatgcg taccaataaa atatcgtggg catttattgc aggcaagcct gttgatttag
     1441 ataatgttca aaaacagttg tatcggaaat acctggataa atttggcttg cacgataatt
     1501 aacgcaatac atacatgcgg taactatcta actttatcaa tataaacaga agtaacgtaa
     1561 atgagattaa cgatgagcca ccgtaactaa aaaacggaaa cggaattcca ataaccggca
     1621 ataagccaat ggccatacca acatttataa atacatgaaa aaataaaatg gaagccacac
     1681 cgtatccata tacccttaca aatgcatttt tttgacgctc ggcaataatt ataatacggt
     1741 aaagtaacac tgtaaaaagc agtaatacag agctggtacc aataaaaccc cactcctcgc
     1801 ctacggtaca aaaaataaaa tcggtactct gctcaggtac aaaatcaaat ttggtttgtg
     1861 tgccttgtaa aaatccttta ccggtaatgc tgcccgaacc aatagcaatc aaactttgat
     1921 tcacattata accggctcct tttaaatcgg tctttttgcc taacagtaca tctatacgtt
     1981 gtttctgatg tggttgtaat gccgtgttat aaacataatc aacactataa accaaaccaa
     2041 tggccaggca acctacaacg gttagcgcta aaaagtttcg ccaggttctg cgtaaagtaa
     2101 accaggtaag caccaaaaca acaactatgg caattattag ataaactttg gctaccatta
     2161 acgaaagcac aaaaagcagt gccgctataa agccaaaaat caaaacgttt tgcgacaacc
     2221 cctcgcgata caacacaaat aaaaatgaag caaaaaccaa agcagacccg gtgtcgtttt
     2281 gcaataagat aatggccatt ggcaacaaaa tcataaagct tgcaattatc ttggttttat
     2341 tttcggcaat acgtatgtat ggcgaactta aatacttagc caaagccagc gctgtagcta
     2401 attttgcaaa ttctgatggc tgtaaattaa acgaccctaa tggtatccag gcatgcgcac
     2461 cttttacatc acgcgataaa aatatggcgc caatattaag ggcaatcatc aatacataaa
     2521 agggtatggc aaatgcttca taaaatttac catcaattat taaaataata atggcaaaca
     2581 atgccgatac ggctatccac attaactgtt ttccatagtt ttgtgttgca tctaaaatac
     2641 tgggatgggc ttcgttgtaa acagccgaaa aaatattaag ccaaccaaac aataccatta
     2701 caatataaat ggaaacggtt ggccaatcaa tattatcaaa tatgcttttt tgttgccgca
     2761 cggtttaaac taaaattaat cgtcaatgtt tgtagctgtg tcttttgcca aaggaactat
     2821 aattgcatct aacatttttt tctctttggc tttgcttgcc tgacaaatac tatccgttaa
     2881 atatttttca atcattaaag tggcaatagg tgcagcaaac catgc
//
LOCUS       DAOMAA010000141         2925 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_27469_length_2925_cov_2.372474, whole genome shotgun sequence.
ACCESSION   DAOMAA010000141 DAOMAA010000000
VERSION     DAOMAA010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27469_length_2925_cov_2.372474"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="PLO59_02755"
     CDS             complement(<1..446)
                     /locus_tag="PLO59_02755"
                     /inference="COORDINATES: protein motif:HMM:NF024181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HQV00048.1"
                     /translation="MKKTILICMAAIAFTACEKNFDTINSNPNGFTTASDGSLFNAAI
                     KSLNFGWNEQLYVNVSVLYKETQLTALPQVRWNNYTLGTEEIWSNYYTTLPNFRELEK
                     RFAAYDTTQAEILNMMAMEKTLLALKTFKVTDMFGDVPFSQAGYGF"
     gene            complement(453..>2925)
                     /locus_tag="PLO59_02760"
     CDS             complement(453..>2925)
                     /locus_tag="PLO59_02760"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQV00049.1"
                     /translation="TPARSGEGQNGYGGGAPDAAYTQPTFQVGIDGIPVHPQLTADAS
                     FGYPGSALSWGPKMEGQEIRWWDGTLKPWSAQPDNIKLPFQTGSQFTHNVSVEGGGDI
                     GTMRVSISRTDNTPIVHNSNFNQTSVATAGNIKVSDKLNVGISSQYVIYNRLNSPMLG
                     ESENSFNKALLYSYPRSWQGEDYYNHTLANGTRNPMDGYPYLYIDKYLWWNYYNRNTT
                     LNRSKLVGGITLNYAITPWLNFMGRSGLDYTDDDYETKGKPSDIVGLQNGYYGIQQGR
                     DKSYNHEFLFTATREKILGSEFNVSLNLGGATWNRDMSSLQARSGTWYFPNWYNLSNY
                     TQPTYGQDSAGNTIVINAGDNISTLVPVTNYYKKRINSVYGFANISYKDFLFVELTAR
                     NDWSSTLPDNANSYFYPGISVSFIATDALNIKTNTLNFLKLRAGASQTATDTDPFQTE
                     FYYKTSLFGSQQASTYPNVIPPTNLKPQRVNDFELGTNMGFFNDRLTLDFTYYYKYAF
                     DQILKTPLPGSAGASSVLINEGAISNNGFEFILNGTILDRKNFTFKAGVNVSRNRNRV
                     ESLGDYADIYILADIWGENGPQMALQEGDFFGTIVGWDYVYKDGKPVVNEAGTAYLLS
                     DKRVPLANAAPHFIAGLTTEFKYKNFSLRTLIDTKWGGSIYSGSYVTGLQTGQSPATL
                     DEREGNGLPFTDPSGNTSNVGVILPGVHADGTPNTTVVHYYYKYMPNAGGWGHFLTRP
                     GIVENTWVKFREIVLAYNMPLKNSKVFRELNLAFTARDLFYIYTTLPDNINPEGLLGA
                     GDAQGFEWGALPGVRSYSLGLNVKF"
BASE COUNT          820 a          597 c          594 g          914 t
ORIGIN      
        1 tgaaaaccgt aaccggcctg gctaaatggc acatcgccaa acatatcggt aactttaaaa
       61 gtttttaatg ccagcaatgt tttttccatt gccatcatat ttaatatttc ggcttgtgta
      121 gtatcgtagg cggcaaatct tttttcgagt tcacgaaaat taggtaacgt tgtgtaatag
      181 ttactccata tttcttcagt ccctaaggtg taattgttcc atcttacttg cggtaaggct
      241 gttagttgtg tttctttata taataccgat acgtttacat acagttgctc attccatcca
      301 aaatttaatg atttgatagc ggcattaaac aacgatccat cgctggcagt tgtaaaacca
      361 ttggggtttg aattaatagt atcgaaattt ttttcgcatg ctgtaaaagc aattgcagcc
      421 atgcatatca gaattgtttt tttcatagct gattaaaatt ttacgtttaa gcctaatgag
      481 taagatctaa caccgggcaa tgcaccccat tcgaaacctt gcgcatcacc ggcacctaat
      541 aacccttcgg ggtttatatt gtcaggcaat gtggtgtaga tgtaaaacaa atcgcgtgca
      601 gtaaaggcaa ggtttagctc acgaaatact ttactgtttt ttagcggcat attataagct
      661 aaaacaattt cacggaattt aacccaggtg ttttctacaa tacccggacg tgtaagaaaa
      721 tgtccccatc cgccagcatt tggcatgtac ttgtagtagt aatgtacaac agtagtattg
      781 ggtgtgccat cggcatgaac accgggcaat atcacaccta cgtttgatgt gttgcccgat
      841 gggtcggtaa agggcaaacc gttgccttcg cgctcatcaa gtgtggcagg gctttggccg
      901 gtttgtaaac cggttacata actgccgcta taaattgagc caccccattt ggtatcaatt
      961 aaagtacgta atgaaaaatt tttatattta aattcagtgg ttaggccggc aataaaatgt
     1021 ggggcagcat tggctaatgg cacacgttta tcgcttaata aataggcagt gcctgcttcg
     1081 ttaactactg gtttgccatc tttataaacg taatcccatc ccacaatggt tccaaaaaaa
     1141 tcgccttcct gcaaagccat ttgcgggccg ttttcgcccc atatatcagc taaaatatat
     1201 atgtcggcat aatcgcctaa actttcaacc cggttacgat tacgtgatac gtttacaccg
     1261 gctttaaatg taaagttttt gcggtctaaa atagttccat ttaaaataaa ctcgaaaccg
     1321 ttattgctta ttgcgccttc gttaatcaat acagacgatg cgcctgccga ccccggaaga
     1381 ggtgttttta atatctgatc gaatgcatat ttgtaatagt aggtaaaatc taatgtaagc
     1441 ctgtcgttaa aaaagcccat gttcgtacct aattcgaaat cgtttacacg ttgtggtttt
     1501 aaattggttg gaggtattac attggggtaa gttgatgcct gttgcgaacc aaacaaactg
     1561 gttttataat aaaactcggt ttgaaacggg tcggtatctg ttgcggtttg tgatgcaccg
     1621 gcacgcagtt ttaaaaagtt taatgtattg gtttttatat tgagtgcatc tgttgcaata
     1681 aaactaaccg atatgcccgg ataaaagtag gagttggcat tatcgggtaa ggttgatgac
     1741 caatcgttac gtgcagtaag ttcaacaaat aaaaaatctt tgtagctgat attggcaaaa
     1801 ccataaactg aattaatgcg ctttttataa taattggtaa ccggcaccag tgtactgatg
     1861 ttatcaccgg catttattac aatggtattg ccggcactat cctgaccata agttggttgc
     1921 gtgtaattgg ataggttgta ccaattagga aaataccacg tacctgagcg tgcttgcaag
     1981 ctgctcatat ctctattcca ggtagcacca cctaagttta aactcacatt aaactcggag
     2041 cctaatattt tttcgcgtgt tgcagtaaac aaaaattcat gattgtaact tttgtcacgt
     2101 ccttgttgta tgccgtagta accgttttga agtccaacga tatcggatgg tttgcctttg
     2161 gtttcataat catcatcggt ataatctaaa cccgaacgac ccataaaatt tagccaaggt
     2221 gtaatagcat aatttaatgt aataccgcca actaatttcg atcggtttaa agtggtgttt
     2281 ctgttgtaat agttccacca taaatacttg tcaatatata aatagggata tccatccatt
     2341 ggattgcgcg taccgtttgc caaggtgtgg ttataataat cttcgccttg ccagctacgc
     2401 gggtatgaat acagtaaagc tttgttaaac gaattttctg actcgcctaa catagggctg
     2461 tttaggcgat tataaattac atactggctg ctgatgccca cattaagttt atcgctcact
     2521 ttaatgttac cggctgtggc taccgaagtt tgattaaaat tgctgttgtg aactatgggc
     2581 gtattatcgg tacgcgaaat agatacacgc atggtcccaa tatcgccacc accttctacc
     2641 gatacattat gtgtaaactg actaccggtt tgaaatggca atttaatatt atcgggttgt
     2701 gcactccatg gttttaaagt accatcccac catcggattt cttgtccttc catttttggc
     2761 ccccatgata gtgccgaacc cgggtagcca aaagatgcat cggctgttaa ttgcggatgt
     2821 accggtatgc catcaatacc tacctgaaac gtaggctgcg tatatgcggc atccggtgcg
     2881 ccaccaccat acccattctg accctctccc gaccgtgccg gtgtg
//
LOCUS       DAOMAA010000142         2912 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_27687_length_2912_cov_3.197060, whole genome shotgun sequence.
ACCESSION   DAOMAA010000142 DAOMAA010000000
VERSION     DAOMAA010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27687_length_2912_cov_3.197060"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="PLO59_02765"
     CDS             <1..501
                     /locus_tag="PLO59_02765"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQV00050.1"
                     /translation="ALSRKIPMDIYASNFEAHNWLSKSWLSRIKHLKFQEAIDVYQIT
                     KQNKGALFALQMYLKLYNSKIVINTHGDNAANVAANMRLTETTGVGACLVTDWKPNLH
                     NFFEPDKEVITYKTVDEAAEKITYLLQHETERISIAQAGKQRTWHNYTMKIKLQHLVP
                     QILQHL"
     gene            515..1288
                     /locus_tag="PLO59_02770"
     CDS             515..1288
                     /locus_tag="PLO59_02770"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HQV00051.1"
                     /translation="MIRSIKKKFLPDEFAKILKQANAYPRFTDKQFAFRHYTFKVTDF
                     LSVAWQLKEIFEDETLYFNTKSTKPTIVDCGANVGIAVAYFKFLYPGCNVVCFEPDKN
                     VFECLKHNIKTNGWENVVVHQAAIYNADTEMFFAGNGADGGSLHAQGQQKVKVLDLAR
                     ILNTCESIDFLKIDIEGAETDVIKHIALQLHKVKYLFVEYHSIKGQPQQLNQLLQVLT
                     EQGFRYYLKSINNLPWKQIANGRSADMDIQLNIHGIKQI"
     gene            1269..2258
                     /locus_tag="PLO59_02775"
     CDS             1269..2258
                     /locus_tag="PLO59_02775"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HQV00052.1"
                     /translation="MALNKFKSAVKQQMLKARHYYDARLGGSCLMLLYHRVADLQTDP
                     QQLAVHPQLFNEQMLFLKLNYNVLRIDEVAWHLNTKTKLPDKSVVISFDDGYADNYLE
                     ALPILELHHLPAVFYIATATLDTNAEFWWDEMERLLLLSKLPDTAQFEMHGLKLDLND
                     FEINRYKKYETVLPILRKTAPEQRQEIINALQQIFGNKTRATHRALTHYELRQFAKSK
                     YVTIGAHTHNHPSLAALDYNLQLLEVEISKKILEDLLKQRIQHFSYPFGTKQDYNTNT
                     LTICNQLQFKSVAANYPYIIHKNSDIFQLPRFLVRNWQLTAFKQQLHFFTTCR"
     gene            2249..>2912
                     /locus_tag="PLO59_02780"
     CDS             2249..>2912
                     /locus_tag="PLO59_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00053.1"
                     /translation="MQIVHLSTDIGGGAGIAALRLHRALLQAGLQSKIITKYGNVTDD
                     THHVIGATTLVNKVAEKLGFLPNWKIQKAETLKKKPPGFEIFSLPYGTIDLSKHPWIQ
                     QADIIHLHWVSDGYINLETLFQLPKQFVWTLHDMNPFTGGCHHADGCLQFENDCSKCP
                     QLHIKQQYLAADAFLFKQKVLKYVGNKLQIIAPSKWLLQLSVQSKLFSDFRHTQIYNL
                     IAQ"
BASE COUNT         1002 a          525 c          532 g          853 t
ORIGIN      
        1 gcattatctc gcaaaatacc tatggatatt tatgcaagta attttgaagc gcataactgg
       61 ttgtcgaaat catggctaag caggataaag caccttaaat ttcaagaagc aattgatgtt
      121 tatcaaatta ccaaacaaaa caaaggagcc ctgtttgcat tgcagatgta tctaaagtta
      181 tacaacagca aaattgtaat taatacccat ggcgacaatg ccgcaaatgt tgctgccaac
      241 atgcgcctta ccgaaacaac cggagtgggt gcctgtttag ttaccgattg gaaaccaaac
      301 ctgcataatt tttttgaacc tgataaagaa gtaattacct ataaaacggt tgacgaagct
      361 gccgaaaaaa ttacctactt acttcaacat gaaaccgaaa gaataagtat tgcccaagct
      421 ggtaagcaac gcacctggca taattatact atgaaaatta agttacaaca tttggtgcct
      481 caaatacttc aacatttgta aagtaagttt taaaatgatt cgttcaatta aaaaaaaatt
      541 tctgccagat gagtttgcaa aaattctaaa acaagcaaat gcctatccac gttttacgga
      601 taagcaattt gcgtttagac attatacatt taaggtaacc gattttttat cggttgcatg
      661 gcagcttaaa gaaatttttg aagacgaaac actttacttt aacaccaaaa gtaccaaacc
      721 aactattgtt gattgtggcg ctaacgttgg catagctgtg gcttatttta aatttttata
      781 tccgggttgt aacgttgttt gttttgagcc tgataagaat gtttttgagt gcctaaaaca
      841 taatattaaa accaatggat gggaaaatgt agtagtgcat caggcagcta tttataatgc
      901 cgatactgaa atgttttttg ccggtaacgg ggctgatgga ggctcgctgc atgcacaagg
      961 acaacaaaaa gtaaaagtgc tcgatttagc acgcatattg aacacctgtg aaagtattga
     1021 ttttttaaaa atagatattg aaggtgccga aacagatgta ataaaacaca tagcgcttca
     1081 gttacataaa gtaaaatatt tatttgtaga atatcatagt attaaaggtc agcctcagca
     1141 acttaatcaa ctgctgcagg tgttaaccga gcaaggtttt cgttactacc tcaaatcaat
     1201 aaacaactta ccctggaaac aaattgctaa tgggcgtagt gctgatatgg atatccaact
     1261 aaacatacat ggcattaaac aaatttaaaa gcgctgtaaa gcaacaaatg cttaaagcca
     1321 ggcactatta tgatgcccgg cttggtggca gttgtttaat gttattgtat cacagagttg
     1381 ccgatttaca aaccgacccg caacagctcg ctgtacatcc gcaacttttt aatgagcaga
     1441 tgttgttttt aaaattaaat tataatgtgc taagaattga tgaagtagcc tggcatttaa
     1501 atacaaaaac aaagctgccg gataaatcgg ttgtaatatc gtttgatgat ggttatgccg
     1561 ataattattt agaagcgctg cctattttgg aattacatca cttaccggct gtattttata
     1621 ttgctacagc aacgcttgat acaaatgcag agttttggtg ggatgaaatg gaaaggttgt
     1681 tattgttaag caaactgccc gatactgcgc agtttgaaat gcatggccta aaattggatt
     1741 taaatgattt tgaaattaat cgttataaaa aatatgaaac agtactgccc atacttcgta
     1801 aaacagcacc tgaacaaagg caagaaatta taaatgcttt gcaacaaata tttggtaaca
     1861 agacccgcgc aacacatagg gccttaacgc attacgaact acggcagttt gctaaaagca
     1921 aatatgtaac cattggtgca cacacacata atcacccttc attggcggct ctggattata
     1981 atttgcaact ccttgaagtt gagatttcga aaaaaatatt agaagattta ttaaaacaac
     2041 gcatacagca tttttcctat ccttttggta ccaaacaaga ctataatacc aatacactta
     2101 ccatttgcaa tcaattgcag tttaaaagtg ttgcagctaa ttatccttac atcatacata
     2161 aaaacagcga tatttttcag ttgccgcgtt ttctggtaag aaactggcaa ctaacagcat
     2221 ttaagcagca attacatttc tttacaacat gcagatagtg catttaagta cagacattgg
     2281 aggtggcgct ggtattgcag cactacgttt gcatcgtgct ttgttgcaag caggtttgca
     2341 atcaaaaatt attaccaaat atggcaatgt tacagatgat acacatcacg taattggagc
     2401 aactacttta gtgaataaag tggcagagaa attgggcttt ttgcccaact ggaaaataca
     2461 aaaggcagaa acattaaaga aaaaaccgcc aggatttgaa atattctcat taccctatgg
     2521 taccattgat ttaagtaaac acccatggat tcagcaggcc gacattattc acctgcattg
     2581 ggttagtgat ggctatatta atttagaaac attatttcag ttgcctaaac aatttgtatg
     2641 gactttgcac gatatgaatc catttacagg cggttgccat catgccgatg gttgcttgca
     2701 atttgaaaac gactgcagca aatgcccgca attgcacatt aaacaacaat accttgcagc
     2761 ggatgcattc ttgtttaaac aaaaagtttt gaaatatgtt ggcaacaaat tgcaaataat
     2821 tgcacccagt aaatggttat tgcaattatc cgtgcaatca aaattgtttt ccgatttcag
     2881 gcatacgcaa atttataacc tcattgcaca ac
//
LOCUS       DAOMAA010000143         2905 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_27815_length_2905_cov_2.427018, whole genome shotgun sequence.
ACCESSION   DAOMAA010000143 DAOMAA010000000
VERSION     DAOMAA010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27815_length_2905_cov_2.427018"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="PLO59_02785"
     CDS             complement(<1..728)
                     /locus_tag="PLO59_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00054.1"
                     /translation="MLPNNKTLFLITIFWICCNHTNAQTITDTCQQSDLPSVLFKNRT
                     KETNSNDRFAMLLLPIIGSTPATGFFGGVGLQIVMRGSYVTDRYSSISGSMQFTAKRQ
                     KLLLAKNTVYLLHNKLFLMGDLRLLLFSQSTYGLGTNAPGKLQKALADLNGYSTNDDS
                     LAQPMSYDYIKIHQTASWLIYPDLYVGGGFHIDKYQDINDIKLDTSSLSPRLTSHYLY
                     NKIEGFNVNKYNLNGVSINAVFDS"
     gene            complement(756..1346)
                     /locus_tag="PLO59_02790"
     CDS             complement(756..1346)
                     /locus_tag="PLO59_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00055.1"
                     /translation="MKRIYLIILFCISINCVNAQRNIFNLNYSVAFPLSDANDFISKT
                     SWRGANLSMLHYLEPGIAVGVETGWNAFYDNVGYTTITDGTQALSGTQYRYGSCVPVL
                     FNLNYYLMPDEVINPFVSLGVGTMYSRSDIDMGMYRARFETWHFALKPELGLQYETGA
                     TSGINFSAKYFHAFKTNELKETRNYLSLNLGFYWKY"
     gene            complement(1351..1983)
                     /locus_tag="PLO59_02795"
     CDS             complement(1351..1983)
                     /locus_tag="PLO59_02795"
                     /inference="COORDINATES: protein motif:HMM:NF024978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HQV00056.1"
                     /translation="MKLKSILSISLFILISCTFFSGCYPEGPTYYEEFDVVYTNYKKD
                     YSFKDKKLFTMPDKIVRITGNIGQGQPPVFISEPYNTQIINRIKSNMIAKGYTFTADT
                     TIADFEILTAAIEVENYQVGYWYDYYYPWYYPGYGGGWYYPYPVVSSYTTGSIGINMV
                     ERKNTSPNNQNAVVWLSIINGLVEGSNIDFSNRIDKCIDQAYTQSPILNQ"
     gene            complement(2005..2631)
                     /locus_tag="PLO59_02800"
     CDS             complement(2005..2631)
                     /locus_tag="PLO59_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00057.1"
                     /translation="MKRLLLLALIITLINNAGFSQGVTLTPTIGYNFDESFNVSGGSG
                     HISDNETYGGILGFDVKQNMAVELSYVRQEAIGKLSYYFGGLQISDNHIPVSLSYLQI
                     GGVKKHPFSPSGKVEGYGGLMLGAFIVNPKDDYDDVWRFAVGFKLGVNIWMTPKVGLK
                     LQTNLQMPIQDWGGSIYVGTGGSGAGVSATSTITQFGFTGGISFRFAE"
     gene            complement(2693..>2905)
                     /locus_tag="PLO59_02805"
     CDS             complement(2693..>2905)
                     /locus_tag="PLO59_02805"
                     /inference="COORDINATES: protein motif:HMM:NF024978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HQV00058.1"
                     /translation="RVQVGTKVEQVPYRNGQLIIDFIDHSTNTVVWRGYAYGSNPIEQ
                     IQIWPVQIRERVDKIINDIFWSCPIK"
BASE COUNT          956 a          564 c          469 g          916 t
ORIGIN      
        1 ctgctatcaa acacagcatt aatgctcaca ccgtttaagt tatatttatt tacattaaat
       61 ccttcgattt tattatatag ataatggctt gttaaccgag gcgacaaact acttgtatcc
      121 agtttaatgt cgtttatatc ttggtactta tcaatgtgaa atccgccacc aacatataaa
      181 tcgggataaa tcaaccacga tgcagtttga tgtattttaa tataatcata gctcatgggt
      241 tgcgccaacg aatcatcgtt ggtgctatat ccattcaaat cggctaatgc tttttgcaat
      301 ttaccgggtg cattggtacc taaaccataa gttgattgcg agaataatag cagacgcaaa
      361 tcgcccatca aaaacaattt attgtgcagc aaatacacgg tgtttttagc caatagtaat
      421 ttttgccgct tggccgtaaa ttgcatacta cccgaaatgc tcgaatacct atcggtaaca
      481 tagctacctc gcataactat ttgcaagcct acaccaccaa aaaaaccggt agcaggtgta
      541 ctaccaataa taggtaagag taacattgca aaacggtcgt tggagttggt ttctttagtc
      601 ctgttcttaa ataaaaccga tggcagatcg ctttgttggc aagtatcagt aattgtttgt
      661 gcattggtat gattacaaca aatccaaaat attgtaatca gaaataatgt tttattatta
      721 ggtaacacaa tacaatatct taaataaaat tggggttaat atttccaata aaagcccagg
      781 tttaacgaca agtaattgcg tgtttctttt agctcatttg ttttgaatgc atgaaaatat
      841 ttagccgaaa aattgatgcc ggatgtagcg cctgtttcat attgcaggcc taactctggt
      901 tttaatgcaa aatgccatgt ttcgaaccgg gctctgtaca tacccatatc aatatcagaa
      961 cgactgtaca tggttccaac acctaaactc acgaatggat ttattacttc gtcaggcatt
     1021 aagtagtagt tcaaattaaa taaaaccgga acacaactgc catatctgta ttgtgtacca
     1081 cttagggcct gtgttccatc agtaattgtg gtgtaaccaa cgttatcgta aaatgcattc
     1141 caaccagttt caacacctac agcaataccg ggttcgagat aatgtagcat acttaagttt
     1201 gcgccacgcc aactggtttt acttataaaa tcgtttgcat cgctcagcgg gaatgcaacg
     1261 ctataattta aattaaaaat attacgttgg gcattaacgc agttaatact tatacaaaac
     1321 aaaataataa gataaattct tttcatgaga ttactgattt aaaattggcg attgtgtata
     1381 agcttgatct atacatttat caatacgatt cgaaaaatct atgttgctgc cttcaaccaa
     1441 tccattaata atgctaagcc aaactacagc attttgatta ttaggacttg tattttttcg
     1501 ttcaaccata tttattccaa tactacccgt tgtataacta ctaactacag ggtaagggta
     1561 ataccaacca ccaccatagc ccgggtaata ccagggataa tagtaatcgt accagtaacc
     1621 cacctgataa ttttcaactt cgatagcagc cgttaatatt tcaaaatcgg ctatggtagt
     1681 atcggcagta aacgtatatc ctttagctat catattactt ttaatacggt taataatttg
     1741 tgtattatag ggttcgctaa taaatacagg aggttgccct tgaccaatgt tgccggtaat
     1801 gcgcacaatt ttatcgggca tggtaaataa ctttttgtct ttaaatgaat agtctttttt
     1861 atagttggta taaactacat caaactcttc gtaataggtt gggccctcgg gataacaacc
     1921 actaaaaaat gtacagctta tgaggataaa taaacttatg ctcaaaatgg attttagttt
     1981 catatactta attaaattat ttatttattc ggcaaagcgg aatgaaatgc cacctgtaaa
     2041 accaaattgt gtaatggttg atgttgctga tacgcctgca cctgaacctc cggtgccaac
     2101 ataaatacta ccgccccaat cttgtatggg catttgtaaa ttggtttgca attttaaacc
     2161 caccttgggt gtcatccaaa tgtttacacc taatttaaag cctactgcaa aacgccacac
     2221 atcatcataa tcgtcttttg ggtttacaat gaaagcgcct aacattaaac cgccatatcc
     2281 ttcaactttt ccagaaggcg aaaacgggtg ttttttaaca cctcctattt gtaaatagct
     2341 taatgaaacc ggaatatggt tgtcagaaat ttgtagccca ccgaaataat acgataattt
     2401 tccgattgcc tcttgtcgca cataacttaa ttcaacagcc atattttgtt ttacatcaaa
     2461 acctaatata ccaccgtatg tctcattatc gctaatatgg cctgaaccgc cactaacatt
     2521 aaacgactcg tcaaaattat aaccaatagt aggtgttaat gtaacacctt gcgaaaaccc
     2581 ggcattatta attaatgtaa ttattagagc aagaagtagg agtcttttca tacttaagat
     2641 tttaaattcg aaatttttta aataaaaaat acaagaaaca atagatataa atctacttta
     2701 ttggacaact ccaaaaaata tcattaataa ttttgtcaac gcgttcacgt atttgaacag
     2761 gccaaatttg tatttgctca atcggatttg aaccataagc ataaccgcgc caaaccactg
     2821 tatttgttga atgatcaata aaatcaataa ttaactgacc attacgatat ggtacctgtt
     2881 caaccttggt acctacctga acacg
//
LOCUS       DAOMAA010000144         2903 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_27851_length_2903_cov_2.426264, whole genome shotgun sequence.
ACCESSION   DAOMAA010000144 DAOMAA010000000
VERSION     DAOMAA010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27851_length_2903_cov_2.426264"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="PLO59_02810"
     CDS             <1..756
                     /locus_tag="PLO59_02810"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HQV00059.1"
                     /translation="RMLSIKQDNIKDFKSAFIPIMLPVLVVCGLILPANFSTAAVLFA
                     CSVCLMFIGRISLKYLGGLMLIGIAGLALFIAIAKFTGYTGRIETWSNRIENFNDKSS
                     GGNFQAEQAKIAIAKGGILGVGPGNSTQRNFLPHPYSDMIFAIVCEEYGLWGALVVVG
                     LYVLLLYRAILLIRQSPGAFATLFAAGCSFGLVLQAMINMAVATNLFPVTGQPLPMLS
                     MGGTSIWFTSLSIGILLSVSRDVNKEGGELAHA"
     gene            749..1858
                     /gene="murG"
                     /locus_tag="PLO59_02815"
     CDS             749..1858
                     /gene="murG"
                     /locus_tag="PLO59_02815"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019387919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HQV00060.1"
                     /translation="MHSIRAIISGGGTGGHIFPAIAIANALKKINPENEILFVGAKGR
                     MEMEKVPAAGYRIEGLWISGFQRKLTLDNLSFPFKLISSLYKAARIIKTFKPNVAVGV
                     GGYASGPLLQQAASKNIPCLIQEQNSYPGITNKILASKVQRICVAYDGMDKYFDATKI
                     VKYGNPVRDEMLAIAGKRNDALAFFKLNTSHKVILVVGGSLGARTINQSIEANLKNFA
                     DTQTQVIWQTGKAYYQTAVAAAKNFSNVVVVDFITRMDLAFAVADIIVSRAGASTLSE
                     LAIIGKPAILIPSPNVAEDHQTKNAQALVKHNAAILVTDTACNSQLWPAINNVLQDDL
                     LAEQLSKNISAMALHNAAHHIAIEVIALAQKQKVQ"
     gene            1855..>2903
                     /locus_tag="PLO59_02820"
     CDS             1855..>2903
                     /locus_tag="PLO59_02820"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HQV00061.1"
                     /translation="MNFDKLKYVYFIGIGGIGMSALARYFKAMGCQVAGYDKTATTLT
                     QQLINEGMQVQFDETVAALPPFINNAKTDELLIVYTPAIPKTHAGLMYLQNKFKIYKR
                     AQVLGFITQNSKTIAVAGTHGKTTTSSIVAHLLVSNGCNCSAFLGGIATNYNTNFISG
                     NAAKPNHVVVVEADEYDRSFLTLFPEVAIVTSMDADHLDIYSNHQTLIQCYHQFVQQV
                     QSGGCIIIKSGLPITTTAAKQISYSTAQPAQAVADNIRIADGNYLFDYKFENVSISDL
                     QLGLPGRHNIENAVAAITAVLQYQLNAMQIRNALQSYQGVKRRYEKIFANNKVQYIDD
                     YAHHPEELRAAILSA"
BASE COUNT          901 a          534 c          602 g          866 t
ORIGIN      
        1 cggatgctat ccataaagca agataatatt aaagatttta aaagtgcatt cattcccatt
       61 atgttaccgg tactggttgt ttgcggttta atattgccgg ctaactttag taccgctgct
      121 gtgttatttg catgcagtgt ttgtttgatg tttataggta gaataagtct gaaatattta
      181 ggcggcttaa tgttaattgg cattgcaggt ttggctttgt ttattgccat tgcaaagttt
      241 acaggttaca ccggccgtat agaaacatgg agcaatcgta tcgaaaactt taacgacaaa
      301 agtagtggcg gaaattttca ggccgagcaa gcaaaaattg ccattgcaaa gggagggatt
      361 ttaggtgttg gccccggtaa ttcaacacaa cgcaattttt taccgcatcc ttattcagat
      421 atgatttttg caattgtttg cgaagagtac gggctttggg gtgcacttgt tgtggtaggt
      481 ctttatgtac tgttactata ccgtgctata ttattaatca gacaaagtcc gggtgcattt
      541 gcaacattgt ttgcagccgg atgtagtttt ggtttggtat tacaagctat gataaacatg
      601 gctgtggcaa ctaatttatt tccggtaaca ggtcaaccgc tgcctatgtt aagtatgggt
      661 ggtacatcaa tatggtttac cagtttatca attggcattt tgctaagcgt tagccgcgat
      721 gtaaataagg aaggaggcga acttgcccat gcatagtatc agagcaataa ttagtggtgg
      781 tggtacaggt ggccacatat ttccggcaat tgcaattgcc aatgcgctaa aaaaaataaa
      841 tcctgaaaac gaaattttat ttgtgggtgc taaaggacgc atggaaatgg aaaaagttcc
      901 ggctgccggc tatcgtatcg aaggcctttg gattagtggt tttcagcgca aattaacttt
      961 agataattta tcgtttccct tcaaacttat cagcagctta tacaaagcag cacgcattat
     1021 taaaacattt aaaccaaatg ttgctgttgg tgtgggtggt tatgcaagtg gtccattgtt
     1081 acaacaagca gcaagtaaaa atataccctg tttaatccag gaacaaaatt catatccggg
     1141 tattacaaat aaaattttag caagcaaagt acaacgcata tgcgttgcct acgatggcat
     1201 ggataaatat tttgatgcaa ctaaaattgt aaaatacggc aatcccgtac gtgacgaaat
     1261 gttggccatt gcaggcaaac gcaacgatgc acttgctttc tttaaattaa acacatcgca
     1321 taaagtaatt ttagttgtag gtggtagttt aggtgcgcgt actataaatc aaagcataga
     1381 agcgaatctt aaaaactttg ctgatacaca aacacaggtt atctggcaaa caggcaaagc
     1441 ctattatcaa acagccgttg cagccgcaaa aaacttttcc aatgtagttg ttgttgattt
     1501 tattacccgt atggatttgg cttttgctgt agccgatatc attgtatcgc gcgcaggagc
     1561 aagcacttta tccgagttgg ctataattgg taaacctgca atattaatcc catcgcccaa
     1621 cgttgccgaa gatcatcaga caaaaaatgc acaagctttg gtaaagcaca atgcggcaat
     1681 attggttact gataccgctt gcaatagcca attatggccg gcaataaaca atgtgttgca
     1741 ggatgatttg ttagcagaac aactttcgaa aaatattagt gctatggcat tacataatgc
     1801 tgcacatcac atagctatcg aagtaattgc cttagcgcaa aaacaaaaag tacaatgaat
     1861 ttcgacaaac taaaatatgt ttatttcatt ggcattggcg gcattggcat gagtgccttg
     1921 gcacgttatt ttaaagcaat gggatgtcag gtagctggat atgataaaac ggctaccaca
     1981 ttaacgcagc agcttattaa cgagggcatg caagtacaat ttgacgaaac tgttgctgca
     2041 ttgccaccat ttataaacaa tgcaaaaacg gatgagttgc ttattgttta tactccggct
     2101 ataccaaaaa cacatgcagg cttaatgtat ttgcaaaata aatttaaaat ttataaacgc
     2161 gcgcaagtgt tgggttttat tacgcaaaac agtaaaacca ttgcagttgc aggtacacat
     2221 ggcaaaacta ccacatcaag tattgttgcg catttgctgg taagcaacgg ttgtaattgc
     2281 tcggcatttt taggcggcat tgctactaat tacaatacta attttattag cggcaatgct
     2341 gcaaaaccca accatgtggt agtggtagaa gccgatgagt atgacaggtc ctttcttacc
     2401 ttattccccg aagtagccat tgtaacttct atggatgccg accatttaga tatctattct
     2461 aatcaccaaa cgcttattca atgttatcat caatttgtgc agcaagtaca atccggtgga
     2521 tgtataatca ttaaaagtgg tttgcccata acaacaactg ctgccaaaca aatcagttat
     2581 tccacagccc aacctgcaca ggctgttgcc gataatattc gcatagctga tggtaattat
     2641 ttatttgatt ataaatttga aaacgtttct atatctgatt tacaattggg cttgccgggt
     2701 cgtcataaca tcgaaaatgc tgtagcagca attacggctg tgttgcaata tcagcttaat
     2761 gcaatgcaaa tccgcaatgc actgcaatcg taccagggtg taaagcgcag gtatgaaaaa
     2821 atatttgcta ataataaagt tcaatacatt gatgactatg cacatcatcc cgaagaatta
     2881 cgtgcagcaa ttttatcagc tca
//
LOCUS       DAOMAA010000145         2902 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_27866_length_2902_cov_2.737267, whole genome shotgun sequence.
ACCESSION   DAOMAA010000145 DAOMAA010000000
VERSION     DAOMAA010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27866_length_2902_cov_2.737267"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="PLO59_02825"
     CDS             <1..212
                     /locus_tag="PLO59_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQV00062.1"
                     /translation="RFRPILMTSLATMLGALPIALALGASAQSRVSMGIAIIGGLLFS
                     LILTLFVIPALYALMSRNANKPATV"
     gene            260..1522
                     /locus_tag="PLO59_02830"
     CDS             260..1522
                     /locus_tag="PLO59_02830"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQV00063.1"
                     /translation="MILHNMQAQYLTVQQAVAITVENNFGILLKQNEIKKLSVNNTAG
                     AAGMLPNLSAGATKTYNVNNTELDFFSGDVRKENGAKSNSTVADATIRWTVFDGLGMF
                     AAKDYLNQLEKAGKADLTAKIEQTIVDVIDKHTEIVLLKKYIATLEENLKLSNARKNI
                     ADKKKQIGTGSALSANQSMVDLINDSTTLITQQINYEKAKVDLNQLMALPPDSPVLVD
                     TAITLIKNFAYDTLLQAAQNQNSILQVARYNKMATADYLRMQQALRLPSINLFGGLAF
                     SQSQAEIGVLERSQNMVVNYGASISIPLFDGGSINRQVATSKIDALNAAINVKDKLLD
                     LETQFNQAVTEHKGLLVILQLSQQNVSIAKSNATIALEQYRNGATTDIDFRATQQIAI
                     VAENDLLTAMYKLKVVESKLLQLSGNLY"
     gene            1568..>2902
                     /locus_tag="PLO59_02835"
     CDS             1568..>2902
                     /locus_tag="PLO59_02835"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQV00064.1"
                     /translation="MQITFYGAAQTVTGSKHLLRLKSGYAVLLDCGMFQGMGAQNLSL
                     NSHLGFDPTQINAVLLSHAHIDHSGLLPLLIKQGYRGVIYSTHATHDLCQLMLLDSAH
                     IQEQDAKFVNKRKSKQNKPHIKPIYTQQDASLALTQFKTVHYNRWIKLNNEVSFMFTD
                     NGHILGAAGISIKVTEDGESKTLFYSGDIGRPHHPILKKPQSFPQADYLIIESTYGNR
                     LHDENTKAQAQLLQEIKRIIDAGKGKIIIPAFSVGRTQEVVYNLDRFISEGKIPAIDV
                     YVDSPLSVNATSVFRKHPEDFNADITNYMMHDDDPFHFSKLHYITDVEDSKALNLLTG
                     PCIIISSSGMTEAGRIKHHIANNVGSADNTILLVGYCPPQTIGGRLADGAPEVRIFGE
                     MYSVKAKVVMLGNFSAHADYLEMIDYLQCQNAELIKKIFLVHGETDVQQAYKQ"
BASE COUNT          974 a          527 c          571 g          830 t
ORIGIN      
        1 aacgtttccg ccctatttta atgacaagtt tagcaaccat gttaggcgct ttgcctatag
       61 ctttggcatt aggagcatcg gcacaaagca gggtaagtat gggtattgct attattggtg
      121 gattgttatt ctctttaata ctaaccttat ttgttatacc ggcattgtat gctttaatga
      181 gtcgtaatgc taataaacct gctacagtat gaccattagt ttccaatata aaataataac
      241 agtttgcctt ttttgtagtt tgatattaca caatatgcag gcgcaatact taaccgtaca
      301 gcaagcagtt gcaataacag tcgaaaataa ttttggtatt ctgcttaaac aaaatgaaat
      361 taaaaagtta agtgtaaaca atactgcagg cgcagcgggt atgttaccaa acctatcggc
      421 aggtgccact aaaacgtaca atgtaaataa caccgaactc gactttttta gcggtgacgt
      481 acgaaaagaa aatggtgcta aatcaaactc aaccgtggcc gatgcaacca ttcgctggac
      541 tgtgtttgac ggcttaggca tgtttgccgc taaagattat ttaaaccaac ttgaaaaagc
      601 cggcaaagcc gatttaacgg ccaaaattga gcaaaccatt gtggatgtaa ttgataaaca
      661 tacggagatt gtattgctaa aaaagtatat agctactttg gaagaaaatt tgaagctttc
      721 caatgcacgc aaaaatattg cagataagaa aaaacaaatt ggtaccggtt cggccctaag
      781 tgccaatcag agtatggttg atttgataaa tgacagtacc acattaataa cgcaacaaat
      841 aaattacgaa aaagctaaag ttgatttaaa tcaattaatg gcattgccgc ccgatagccc
      901 ggtgcttgta gatacagcaa ttacgcttat taaaaatttt gcatacgata ctttattgca
      961 agcagcacaa aatcaaaaca gtatattgca agtagcgcgt tataataaaa tggcaactgc
     1021 tgattattta cgcatgcaac aagctttgcg cttacctagt ataaatttat ttggtgggtt
     1081 ggctttcagc caatcgcaag ccgaaattgg cgttttagag cgaagccaaa atatggtcgt
     1141 caattatggt gcttcaatta gtattccgct atttgatgga ggtagcataa accgacaggt
     1201 agccacatct aaaatagatg cattaaatgc agctatcaat gtaaaagata aattattaga
     1261 cttagaaaca cagtttaatc aggctgtaac agaacacaaa ggtttgttgg ttattttgca
     1321 actaagtcaa caaaatgtta gcatagctaa aagcaatgca accattgcat tggaacaata
     1381 tcgaaacggt gccactaccg atattgattt tagagctacg caacaaatag ccattgtagc
     1441 cgaaaacgat ttgcttacag caatgtataa acttaaagtt gtcgaaagta aattgttgca
     1501 actaagtggt aatttgtatt aataaattgc ggatgttcca actattaaaa ctttaatttc
     1561 tcactgtatg caaataacat tttacggagc tgcacaaact gttaccggta gcaaacacct
     1621 gcttcgctta aaaagcggat acgctgtttt gcttgattgt ggcatgtttc aaggtatggg
     1681 ggctcaaaat ttaagtttaa acagccacct cggtttcgac ccaactcaaa tcaatgcagt
     1741 gttattatcg catgcacata ttgaccatag tggtttgtta cccttgttaa taaaacaagg
     1801 gtatcgtggt gttatttaca gcacacatgc aacacatgac ttatgccaac ttatgttact
     1861 tgatagtgca catatacagg agcaggatgc caagtttgta aacaaaagaa aaagcaaaca
     1921 aaataagcca cacatcaaac ctatatatac gcagcaagat gcatcgttag cactaacgca
     1981 gtttaaaaca gtgcattata accgttggat aaaactcaat aacgaggtgt cgtttatgtt
     2041 taccgataat gggcatattt taggtgcagc gggtataagt attaaagtaa cagaagatgg
     2101 cgaatcgaaa acactttttt attcaggtga tattggccgg ccacatcacc ccattctgaa
     2161 aaagccacag tcatttccgc aagccgatta tttaattatt gaatcaactt atggcaaccg
     2221 cctgcacgat gaaaatacaa aagcacaagc acaattgctg caggaaatta aacggattat
     2281 tgatgccggt aaaggcaaaa taattattcc ggcatttagt gttggccgta cacaagaagt
     2341 ggtttataat ctcgatcgtt ttatttcaga aggcaaaata ccggctattg atgtttatgt
     2401 tgatagtcct ttgtcggtaa atgccacatc ggttttcaga aaacaccctg aagattttaa
     2461 tgccgatatt accaattaca tgatgcacga tgacgatccg tttcattttt cgaaactgca
     2521 ttatataacg gatgtggaag actcgaaggc attaaatcta ttaacagggc catgtatcat
     2581 catatcatca tcggggatga cagaagccgg ccgcatcaaa catcatattg ctaataatgt
     2641 gggtagtgcc gataatacaa ttttgctggt aggatattgc ccgccacaaa ctattggtgg
     2701 ccgtttagcc gatggtgcgc ctgaggtgcg catatttggt gaaatgtatt ctgttaaagc
     2761 caaggttgta atgctgggta actttagtgc ccatgccgat tatcttgaaa tgatagatta
     2821 tctgcaatgc caaaatgccg aactgattaa aaaaatattt ttagtgcatg gcgaaacaga
     2881 tgttcaacaa gcatataaac ag
//
LOCUS       DAOMAA010000146         2882 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_28233_length_2882_cov_2.512911, whole genome shotgun sequence.
ACCESSION   DAOMAA010000146 DAOMAA010000000
VERSION     DAOMAA010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28233_length_2882_cov_2.512911"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="PLO59_02840"
     CDS             complement(<1..414)
                     /locus_tag="PLO59_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00065.1"
                     /translation="MKKQLISLAAVLAATSFTFNAQAQWLVGGNALGSTGNFGSTTNQ
                     DVNFISNNLTRMTLTKAGLLGIGTTAPGFLLDVQTPGNASANFKSGTGTANLIIDRGN
                     STATSSVSYRTAGTPTWQTGTLGTDNFSIRNIALGS"
     gene            575..1546
                     /locus_tag="PLO59_02845"
     CDS             575..1546
                     /locus_tag="PLO59_02845"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HQV00066.1"
                     /translation="MQRTKLIILIVFTYVLLQFSWWAYMLVKLNAALYEQKIELANLN
                     SSIDDAYQTKQNLDASLKLKYWMIAGEGLVFIGLMVFGISKLLNAYNKEMDLAQRQKN
                     FLLSITHEFKSPLAAVRLNMQTMQKHQLDDEKRKMVITSTLLEANRLNTLVENALMAA
                     QIETHNFVQSTTPFNFSKCISTQMQARALVSKKIKTCYTNIEPEVYITGDCISLSSVV
                     FNIVENAEKYTADEARIEVHLYKKHKEAELIIKDNGFGIAPEERDKIFEKFYRIGNED
                     TRKSKGTGLGLYIVKKLVEIHHGKIAVRENYPQGTVFQINFPLVNAE"
     gene            1583..>2882
                     /gene="gpmI"
                     /locus_tag="PLO59_02850"
     CDS             1583..>2882
                     /gene="gpmI"
                     /locus_tag="PLO59_02850"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HQV00067.1"
                     /translation="MGKKVALLILDGWGEAPDNASNAIAAADTPFMDSLYQNQQHAQL
                     LTCGQHVGLPDGQMGNSEVGHMNIGAGRIVWQELARINREIDLGLIEKNKTLLSLFDY
                     CKTNSKPLHLMGLVSDGGVHSHINHLFVLVKLAHTHGITTFIHAFTDGRDTDPKSGLG
                     FINDLQQFINANNLQSKIASICGRYYAMDRDKRWERVAIAYNALVNAQGDLEIDAAQA
                     IQKSYAAGITDEFIKPIIITDNNKPTATIKAHDAVLCFNFRTDRCREITQVLSQCNMP
                     EFNMQTLPLYYVTFTRYDERFEGVEVIYEKDNLNLTLGEVIANANKTQVRIAETEKYP
                     HVTFFFSGGREATFDKEQRIMIPSPKVATYDLAPQMSAPLITEAIVATIKTNAPDFIC
                     LNFANTDMVGHTGVFNAVMQAAQTVDTCTATVTNAAIENNY"
BASE COUNT          964 a          509 c          573 g          836 t
ORIGIN      
        1 tgaacccaaa gcaatattgc gaatcgaaaa attatcagta cccaaggtac ccgtttgcca
       61 tgtaggtgtg ccggcagtac gataacttac tgatgaagta gctgttgagt ttccacggtc
      121 aataattaaa tttgctgtac ccgtacccga tttaaagttg gcactggcat tacccggagt
      181 ttgaacatcc aataaaaagc ccggtgctgt ggtaccaata cctaaaaggc ctgccttggt
      241 aagcgtcata cgcgtaaggt tgttactaat aaagtttaca tcctgattgg tggttgaacc
      301 aaagttgccg gtgctcccta atgcattacc ccctacaagc cattgcgctt gtgcattaaa
      361 agtaaacgat gtggcagcta atacagcagc caaactaatg agttgttttt tcataataaa
      421 taaatttttt ggtgaaggtt ttttttgaat aagccaatgt gcttatctat ttaaagcaat
      481 tataagtaaa atttgtgtaa ctccacaaca agcgttgata aacattattt gtttgcgctt
      541 taaaaataaa tgatccttgt ttttaatttt aaacatgcaa agaaccaagc ttataatatt
      601 aattgttttt acctatgtat tgcttcagtt tagctggtgg gcatacatgt tggtaaaact
      661 taatgcagca ttgtatgaac agaaaattga gttggcaaat ttgaatagct caattgatga
      721 tgcttatcaa acgaaacaaa atttagatgc ttcgctgaaa ctaaaatatt ggatgatagc
      781 tggcgaaggt ttggttttta tcggattaat ggtgtttggc atttcaaaac tacttaatgc
      841 ttataataaa gagatggacc tggcacagcg acaaaaaaac tttttgctat ctatcacaca
      901 tgaatttaag tcgccattag ctgctgtacg gcttaatatg caaaccatgc aaaaacatca
      961 attagatgac gaaaagcgca aaatggttat cacatcaacc ttgttagaag ccaacagatt
     1021 aaacaccctg gttgaaaatg cattaatggc tgcacaaatc gaaacccata attttgtgca
     1081 aagtactacg ccttttaatt tttcgaaatg tataagtacg caaatgcagg cacgagcctt
     1141 ggtttcaaaa aaaattaaaa cctgttatac caatatcgaa cctgaagtat atataacagg
     1201 cgattgtatt tcattaagct ctgttgtatt taatattgtt gaaaatgccg aaaaatatac
     1261 ggctgatgaa gcacgtattg aagtgcactt atacaaaaaa cataaggaag ccgaattaat
     1321 aattaaagac aatggttttg gtattgcacc ggaagaacgt gacaaaattt ttgaaaaatt
     1381 ttatcggatt ggcaatgaag atacccgcaa aagcaaggga accgggctgg gactttacat
     1441 tgtaaaaaaa ttggtggaaa tacatcatgg taaaattgca gtaagagaaa attatccgca
     1501 aggcaccgta tttcaaatca attttccttt agtaaatgct gaataaaatt ttgttgtatt
     1561 gcattaataa atctaacaat acatgggaaa aaaagtagca cttttaattt tagatggatg
     1621 gggcgaggct ccagataatg ccagtaatgc cattgcagca gccgatacac catttatgga
     1681 tagcctttat caaaatcagc aacatgcaca actgttaacc tgtgggcaac atgtgggctt
     1741 gcccgatgga cagatgggta actctgaagt aggccacatg aatattggtg ccgggcgtat
     1801 tgtttggcag gagttagcac gcattaaccg tgagattgat ttgggattaa ttgaaaaaaa
     1861 taaaacactg ctaagcctgt ttgattattg caagacaaat tcaaaaccgt tgcacttaat
     1921 gggtttggtt agcgatggtg gtgtgcattc acatataaac catctgtttg tcttagttaa
     1981 gttggcacat acacatggca taactacatt cattcatgca tttaccgatg gccgcgatac
     2041 ggaccccaaa agtggcttag gatttataaa tgatttacaa caatttatta atgcaaataa
     2101 tctgcaatca aaaattgcaa gcatttgcgg tcgttattac gcaatggata gagataagcg
     2161 atgggagcgt gtggccattg cttataatgc acttgtaaat gcccaaggcg atttagaaat
     2221 tgatgctgca caggcaatac aaaaaagtta tgctgccggc ataaccgatg aatttataaa
     2281 accaattatt attactgata ataacaagcc cacagcaacc attaaagcac atgatgccgt
     2341 actatgtttt aatttcagaa ccgaccgttg tcgtgagata actcaggtat tatcgcagtg
     2401 taatatgccg gagtttaata tgcaaacatt gccattatac tatgttacat ttacaagata
     2461 tgacgaacgt tttgaagggg tggaggtaat ttatgaaaaa gataatttaa acctgaccct
     2521 tggcgaagtt attgccaatg ccaacaaaac acaggtgcgg attgccgaaa ctgaaaaata
     2581 tccgcacgtt acttttttct ttagtggcgg gcgcgaggca acatttgata aagaacagcg
     2641 catcatgatt ccatcgccca aggtagctac ttacgatttg gcaccacaaa tgagcgcgcc
     2701 attaattact gaagctattg tagcaaccat taagacaaat gcccccgatt ttatttgctt
     2761 aaattttgcc aataccgata tggttggcca cacaggtgtt tttaatgcgg taatgcaagc
     2821 tgcgcaaacg gttgatacat gtacagcaac agttacaaat gctgcaatcg aaaataatta
     2881 cg
//
LOCUS       DAOMAA010000147         2878 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_28299_length_2878_cov_3.245838, whole genome shotgun sequence.
ACCESSION   DAOMAA010000147 DAOMAA010000000
VERSION     DAOMAA010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28299_length_2878_cov_3.245838"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1711)
                     /gene="secA"
                     /locus_tag="PLO59_02855"
     CDS             complement(<1..1711)
                     /gene="secA"
                     /locus_tag="PLO59_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008507536.1"
                     /note="functions in protein export; can interact with
                     acidic membrane phospholipids and the SecYEG protein
                     complex; binds to preproteins; binds to ATP and undergoes
                     a conformational change to promote membrane insertion of
                     SecA/bound preprotein; ATP hydrolysis appears to drive
                     release of the preprotein from SecA and deinsertion of
                     SecA from the membrane; additional proteins SecD/F/YajC
                     aid SecA recycling; exists in an equilibrium between
                     monomers and dimers; may possibly form higher order
                     oligomers; in some organisms, there are paralogous
                     proteins that have been found to be nonessential but do
                     function in secretion of a subset of exported proteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HQV00068.1"
                     /translation="MLDLINKTLGKFFGTKTDRDLKELNPVATTVNKEFAQLENISND
                     ELRAHTQSFKDTIAQRTAETDAAITQLQTEAEQDPDMDATAKEDLYQSIDKLKKQRLQ
                     IIEEVLTEIHPQAFAVVKEAARRFKNNAQLEVTATPHDRQLATYRKNVTIDGDKAYYA
                     NQWLAAGNTVTWDMVHYDVQLIGGTVLHQGKIAEMATGEGKTLVATLPIYLNALAGCG
                     VHVVTVNDYLAKRDSEWNGPLFEFLNLTVDCIDKHQPNSDNRRNAYLADITYGTNNEF
                     GFDYLRDNMARAPEDMVQRQHHYAIVDEVDSVLIDDARTPLIISGPTPRGDDQMFHAL
                     KPNVEILINAQRNYLNTAIADAKKKFGEKNNDDAGLALLRSYRGLPKSKPLIKFLSED
                     GVRVLLQKTENFYMQDQSKEMHKVDAELYFVIDEKSNQVELTEKGIELISANTDKDFF
                     IVPDMGSFIAELDKLNLSDTEKFERKEALMRDFSVKSERIHTITQLLKAYTLFERDTE
                     YIISEDGKVKIVDEQTGRVLEGRRYSDGLHQAIEAKENVKIEAATQTYATVTLQNYFR
                     MYHK"
     gene            complement(1903..2664)
                     /locus_tag="PLO59_02860"
     CDS             complement(1903..2664)
                     /locus_tag="PLO59_02860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQV00069.1"
                     /translation="MLKISIITITYNSAQTIEATLQSLLMQDYKNVELIIIDGKSTDG
                     TLSIVEKYRDHIAQVVSEKDDGLYFALNKGISLCTGDIIGILHSDDVYYNAQVLTNVA
                     YAFTNNNTAEALYGDLIFVDRNNLSKIKRVWRSGAYKPDAFKWGWMPPHPTFFVKTQV
                     YKKYGVFNTQLRYAADYELMLRFIHVRKIKIIYLPQIMVRMRLGGISNFSINNKLIAN
                     KEDHKAWQLNGIKPNVFTLLLKPLSKLNQYLLRHI"
     gene            complement(2691..>2878)
                     /locus_tag="PLO59_02865"
     CDS             complement(2691..>2878)
                     /locus_tag="PLO59_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HQV00070.1"
                     /translation="APIHLQHGVWIGCNAVVCPGVTAQQHAVLTVASVAVKNLDAFGI
                     YTGNPSIKIKQRVIENT"
BASE COUNT          776 a          558 c          548 g          996 t
ORIGIN      
        1 gtttgtggta catcctaaag tagttttgca gtgtaacggt tgcatacgtt tgcgtagcag
       61 cctcaatttt tacattttct tttgcttcaa ttgcctgatg taatccatcg ctgtaacgtc
      121 tgccttcgag cacacggccg gtttgctcgt caactatttt aaccttaccg tcttcgctaa
      181 taatatattc tgtatcgcgt tcaaaaagtg tgtaagcttt tagtaattgt gttatggtat
      241 gtatgcgttc gctttttacc gaaaaatcgc gcatgagggc ttctttgcgt tcaaactttt
      301 cggtatccga aaggtttagt ttgtcaagtt cggcaataaa actacccatg tcgggcacta
      361 taaaaaagtc tttatcggta tttgcgctta tcagttcaat acctttttcg gtaagttcaa
      421 cctgattgct tttttcatca attacaaagt aaagttcggc atctacttta tgcatttcct
      481 tgctttggtc ttgcatgtaa aagttttcgg ttttttgtaa taaaacgcgc acgccatctt
      541 cgcttaaaaa ttttatcaag ggtttgcttt tgggtaaccc tcggtatgaa cgtagtaatg
      601 ccaagccggc atcgtcatta tttttttcgc caaatttttt ctttgcatca gcaattgccg
      661 tattgaggta attgcgttgt gcatttatta aaatttcaac gttgggtttt aatgcatgaa
      721 acatttgatc gtcaccgcgt ggcgtagggc ccgaaataat taagggtgta cgtgcatcat
      781 caattaatac actatcaacc tcgtccacta tggcatagtg atgttggcgt tgtaccatat
      841 cttcgggtgc gcgtgccatg ttatcgcgca ggtagtcgaa accaaattcg ttgttggtgc
      901 cataggtaat atctgccaga tatgcatttc tgcgattatc gctattgggt tggtgtttgt
      961 caatgcaatc aacggtaagg tttaaaaact cgaataacgg cccattccat tcgctatcgc
     1021 gtttagctaa ataatcgttt accgttacca cgtgcacacc acatcctgcc aatgcattta
     1081 agtatatggg taaggttgcc accaatgttt ttccttcgcc tgtggccatt tcggcaattt
     1141 tgccctgatg taacaccgta ccgccaataa gctgcacatc gtagtgtacc atatcccagg
     1201 ttacggtatt gcctgctgca agccactgat tggcgtagta tgctttatca ccatcaatgg
     1261 ttacgttttt acgataggtt gccaattgtc tgtcgtgtgg cgttgccgta acttctaatt
     1321 gggcattgtt tttaaatcta cgcgcagctt ctttaactac agcaaaagct tgcgggtgaa
     1381 tttcggttaa tacttcttcg ataatctgaa gcctttgttt ttttaattta tcaattgatt
     1441 gatataaatc ttccttagcg gttgcatcca tatccggatc ttgctcggcc tcggtttgaa
     1501 gttgtgttat tgcagcatcc gtttcggcag tgcgttgggc aatggtatct ttaaatgatt
     1561 gggtatgtgc ccgtagttca tcattactta tgttttcgag ttgggcaaac tccttattaa
     1621 ctgtagttgc tacggggttt aattctttaa gatcgcgatc ggttttggta ccaaaaaact
     1681 tacccagggt tttatttatt aagtctaaca ttttactctt tattaaatta attattgcca
     1741 agtagctata cagcttcaaa gcatgttgtg tttacaacaa cttagccatc aaaactaaaa
     1801 ggacattatg gcacaacttg gtataaacgg ctggcgaaag taaatattta cgttgtttaa
     1861 aaaagtaaca acggtgcatt aaaaagcctg ttaaacaaac ctttaaatat ggcgcagtaa
     1921 atattgatta agttttgaaa gtggcttaag caacaaggta aatacatttg gcttaatgcc
     1981 atttaattgc caggctttgt ggtcttcttt attagcaatt agcttattat ttatggagaa
     2041 attgctgata ccgcctaaac gcatacgaac cataatttgt ggcaggtaaa ttattttaat
     2101 ttttcttaca tgaataaaac gaagcattaa ttcgtaatcg gccgcatatc gaagttgtgt
     2161 attaaacaca ccatattttt tgtacacctg tgtttttacg aaaaaggttg gatgtggcgg
     2221 catccagccc catttaaatg catctggctt gtaagcccca ctacgccaaa cccgttttat
     2281 ttttgagagg ttgtttctgt ccacaaatat taaatcacca tacaaggctt cggcagtgtt
     2341 attattagta aaagcataag ctacatttgt taatacttgt gcattataat acacatcgtc
     2401 actatgtaaa atgccaataa tatcgcctgt acacaggcta atgcctttat ttaaagcaaa
     2461 atataaacca tcgtcttttt cagaaactac ttgtgcaata tgatcacgat atttttcaac
     2521 tattgacagg gttccgtctg ttgatttgcc atcaataatt ataagctcca catttttata
     2581 gtcttgcatc agtaacgact gaagcgtggc ctcaatagtt tgtgcactgt tataagttat
     2641 ggttatgata gaaattttaa gcactttaat tggattatga aaagtaaata ctaagtgttt
     2701 tcaattaccc tttgttttat tttaatactt gggttgcctg tataaattcc aaacgcatct
     2761 aaatttttaa ctgctacaga cgcaaccgta agtactgcat gttgctgtgc tgtaacaccg
     2821 ggacatacaa ctgcattaca ccctatccaa acaccgtgct gtaaatgaat gggcgcta
//
LOCUS       DAOMAA010000148         2874 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_28382_length_2874_cov_3.494856, whole genome shotgun sequence.
ACCESSION   DAOMAA010000148 DAOMAA010000000
VERSION     DAOMAA010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28382_length_2874_cov_3.494856"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2874
                     /locus_tag="PLO59_02870"
     CDS             <1..>2874
                     /locus_tag="PLO59_02870"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HQV00071.1"
                     /translation="ALGAAGSMGSTTNYDVNFITNNLNRMTLTKAGLLGIGTTTPGFL
                     LDVQATGNASANFKSATGTANLIIDRGSATATSSVSYRTAGTPTWQTGTLGTNNFAIR
                     NSALAQPAMVVLATNNYIGIGTTTPTARFQVSDDVKFHGVRVGLGGGGDAYSTVVGYE
                     TLLNNAGGSGNTAIGYRVLNSNTTGSSNSAFGLESLKSNTIGHNNSGIGAYTLNYNSE
                     GSNNIAIGSYAMYSNTTANNNTSIGIYSMSNNVTGEANVALGLYALSFNQTGNDNTAI
                     GSRAIYSNSSGYSNVALGTSCLYKNVTGSNLVAVGDSALYNLNGGVGQNTAIGSKSQY
                     ATTTGAYNTSLGYNSLMANTTGSSNSALGSAALNFNTTGYGNAAMGSYALFFNTTGYN
                     NSAMGSGALYSNTTGYHNAALGVSALYYNATGNGNSSVGNYTLYNNTTGNYNSVLGYL
                     SMNSNVSGHNNSAIGYSALYYNTSGSNNAAVGYYSLTFNTTGYSNVAMGTSCLYKNVT
                     GSNLVAVGDSALYNLNGGFGQNTAIGSKSQFATTTGAYNTSVGFETLRDNTLGSNNTA
                     IGSAALFANTTGNYNIATGSFSLFANISGYYNTATGYEVLFPNTSGSWNAGHGYLSLR
                     TNTTGYQNAGFGAYSLYNNTTGFRNAALGYGSDVSSGALNNATALGSLVIVNASNKVR
                     VGNSTVTVNEINAGVWTTSDGRFKYDVKEEVKGLDFINALRPVVYNFDAWKFEEFLRQ
                     NYPAERRTEQLNEMKDGLKRASDIRQTGFIAQEVEEAAKRVGYNFNGVHHPETQEDNY
                     SVAYDKFVVPLVKAVQELSVDNNKLKAENEKITIEGEKMKIEIKSLETTVAEMKACIE
                     TLCQSSLQKTVTPINESGSYLRQNNPNPFNTHTVINYVIAAKATTAMLKIFSVNGIEL
                     KSYKINTQGPGQIEIAAHAFTPGVYTYILIVDDNTIDTKQMVVT"
BASE COUNT          943 a          530 c          610 g          791 t
ORIGIN      
        1 gccttaggtg cagccggcag tatgggtagc acaaccaatt acgatgtaaa ctttattacc
       61 aacaacttaa accgtatgac gcttaccaaa gccggcttat taggcattgg tacaactacg
      121 cccggttttt tattagatgt gcaggcaacc ggtaatgcca gtgctaattt taaatcggct
      181 acgggtacag ccaatttaat tattgaccgt ggcagtgcta cagctacctc atcagttagt
      241 taccgtacag caggcacgcc aacatggcaa acaggtactt tgggtactaa taattttgcc
      301 atacgcaaca gcgcattggc acaacctgct atggttgtac ttgcaaccaa taattacata
      361 ggcattggaa caacaacgcc aacggcacgg tttcaggtaa gtgatgatgt aaagtttcat
      421 ggggtgcgtg taggcttagg tggtggcggt gatgcgtata gtaccgtagt tggttatgag
      481 acattgctaa acaatgctgg cggcagtggt aatacagcaa ttggttaccg tgttttaaat
      541 tcgaatacaa ccggtagtag caattcggct ttcggccttg aatcattaaa atcaaacact
      601 attgggcata ataatagcgg cattggtgct tatacattaa attacaactc cgaaggatca
      661 aacaatatcg caataggtag ctatgcaatg tatagcaaca ccacggcaaa caacaataca
      721 tcaattggta tttactcgat gtcgaacaac gtgacaggtg aagcaaatgt agctttagga
      781 ctatatgcat tgagctttaa ccaaaccgga aatgacaata cagctatagg ctcgcgtgct
      841 atctattcaa actcctcagg ttattcaaat gtagccctgg gcacaagttg tttatataag
      901 aatgttacag gtagcaacct ggtagccgta ggcgactctg cattgtacaa cttaaacggt
      961 ggtgtgggcc aaaatacggc cataggtagt aaatcgcagt atgctacaac aacaggtgct
     1021 tacaatacct cactgggtta taattcatta atggcgaata caacaggttc atctaactca
     1081 gctttgggta gcgctgcatt aaatttcaat actacaggat atggaaacgc tgctatgggt
     1141 agttatgcat tattttttaa cacaacgggt tataataatt cagcaatggg ttcaggcgct
     1201 ttgtatagca atacaacagg ctatcataat gcggctttag gtgttagcgc attgtattac
     1261 aacgcaacag gcaatggaaa ttcatctgtt ggtaattata cattatataa caatacaaca
     1321 ggtaattata attcagtgtt aggttattta tcaatgaatt ccaatgtttc tgggcataac
     1381 aattcagcca ttggatatag tgcattatat tacaatactt ccggcagcaa taatgcggca
     1441 gtaggctatt attcattgac ttttaatact accggttact caaatgtagc catgggcaca
     1501 agttgtttat ataaaaatgt tacaggtagc aacctggtag ccgtaggcga ctctgcattg
     1561 tacaacttaa acggaggttt tggccaaaat acggccatag gtagtaaatc gcagtttgct
     1621 acaacaacag gtgcttataa tacttctgtg ggttttgaaa ctttaagaga taatacactt
     1681 ggctccaaca atacagctat aggtagtgcg gccttatttg ccaatacaac aggcaactat
     1741 aatattgcaa ccggaagttt ttcgttattc gctaacataa gtgggtacta taatacagca
     1801 accggttatg aagttttgtt tcccaatact agtggatcgt ggaacgcagg ccatggatat
     1861 ttatcattaa gaactaatac tacaggatat caaaacgctg gttttggggc ctattcttta
     1921 tacaataaca ccaccggttt tcgtaatgca gcacttggat atggtagcga tgtatcgtca
     1981 ggtgcattaa acaatgcaac agcgcttggt tctcttgtta tagtaaatgc cagtaataaa
     2041 gtgcgtgtag gtaacagtac ggttacggta aacgaaataa atgcaggtgt gtggacaacc
     2101 agcgatggcc gttttaagta tgatgtaaaa gaagaagtaa aaggtttgga ttttataaat
     2161 gctttacgcc ctgtagtgta taattttgat gcatggaagt ttgaggaatt tttacgtcag
     2221 aattaccctg ccgaaagaag aacagaacaa ttaaacgaaa tgaaagacgg attaaaacgt
     2281 gccagcgata tacgccaaac cggatttata gcacaggaag ttgaagaagc tgcaaaacgt
     2341 gtgggttata actttaacgg tgtgcaccat cccgaaaccc aggaagacaa ttacagtgta
     2401 gcgtatgata agtttgtagt gccattggta aaagccgtgc aggagttatc agttgataat
     2461 aataagttga aagctgaaaa tgaaaaaatt acgatagagg gtgagaagat gaaaattgaa
     2521 ataaaaagct tggaaacaac cgtagccgaa atgaaagcat gtattgaaac actatgccag
     2581 tcatcgttac aaaaaacagt tacaccaata aatgaaagcg gaagttattt acgccaaaac
     2641 aaccccaatc catttaacac acataccgta ataaattatg ttatagctgc caaagcaact
     2701 acggctatgc ttaaaatatt ttcagtaaat ggtatcgaat taaaatccta taaaatcaac
     2761 acccaaggcc cgggtcaaat agaaattgca gcacatgcct ttacacccgg tgtttatacc
     2821 tacattttaa ttgttgatga taataccatt gataccaaac agatggtggt tacg
//
LOCUS       DAOMAA010000149         2874 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_28391_length_2874_cov_2.763391, whole genome shotgun sequence.
ACCESSION   DAOMAA010000149 DAOMAA010000000
VERSION     DAOMAA010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28391_length_2874_cov_2.763391"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..878)
                     /gene="pyrF"
                     /locus_tag="PLO59_02875"
     CDS             complement(54..878)
                     /gene="pyrF"
                     /locus_tag="PLO59_02875"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009437613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HQV00072.1"
                     /translation="MNKQTLFKNIEQKQSFLCIGLDVDIEKIPAHIKQLPDAIFQFNK
                     AIIDATHTYCVAYKPNTAFYECYGVSGWQALQQTIDYIKQNYPDQFIIADAKRGDIGN
                     TSAMYAQAFFKHMQADAVTVAPYMGADSVKPFLNFSDKWVVLLALTSNSSAADFQLQT
                     LADSQPLYKHVLHQSLQWGNADNMMYVVGATQAPLLAEIRKIIPQHFLLVPGVGAQGG
                     SLAEVAQYGLNSSCGLLVNASRSIIYASNDLSFAKAAGREAQKMQLEMAYLLQKFS"
     gene            complement(899..1969)
                     /gene="prfA"
                     /locus_tag="PLO59_02880"
     CDS             complement(899..1969)
                     /gene="prfA"
                     /locus_tag="PLO59_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HQV00073.1"
                     /translation="MLDKLEAIEIKFKEIEDQLSAPDVISDMKRFAALNRQYKEIKGL
                     VEKTQEYKLVLNHYDEAHHLLNTEKDDDIKEMAKAELETLEEKRNNLEDEIRYLLIPA
                     DPEDSKNAIFEIRAGTGGDEASIFAGDLYRMYTRYCEARGWKLELVDYSAGSTGGYKE
                     IIFNILGEGSYGILKYESGVHRVQRVPLTETQGRVHTSAATVAVLPEADEFDVEVKEA
                     DIRKDYFCSSGPGGQSVNTTYSAVRLTHIPTGIVVQCQDEKSQIKNYAKALGVLRSRI
                     YEVELQKRQDEIAKKRKTMVSSGDRSAKIRTYNYAQSRVTDHRIGLSTFNLPGFMDGD
                     IQSFIDALQLAENAEKLKEAIV"
     gene            2167..2589
                     /locus_tag="PLO59_02885"
     CDS             2167..2589
                     /locus_tag="PLO59_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00074.1"
                     /translation="MNFLKLTVAAVVLNTTAYAQTEAEMAAKPVTKTEAVKERATYEE
                     RATKQVNRISEIVHLDDMQHQTIKALYLESYPKIKEVHKAAGKDTARADAESKKIMKE
                     RRKNVEALLNKEQKNKLEAYIKANKDIDADDEIKATSK"
BASE COUNT          850 a          597 c          539 g          888 t
ORIGIN      
        1 gtgcaaggta ataacaagat ggcaagcatg cgcaaagcct aaaagtaaaa gcgttagcta
       61 aatttttgca acaagtaagc catttcaagc tgcatttttt gagcttcccg gcctgcagct
      121 ttcgcaaaac ttaaatcgtt gcttgcgtaa ataatactgc gcgatgcatt aaccaataat
      181 ccacacgaac tatttaatcc atattgagca acttcggcca ggctgccacc ttgtgcacct
      241 acaccgggaa ccagtaaaaa atgttgaggt attatttttc tgatttcggc aagcaatggt
      301 gcttgtgtgg ctccaactac atacatcata ttatcggcat tgccccattg caacgattga
      361 tgtaaaacat gcttgtaaag cggttgcgaa tccgccaggg tttgtaattg aaaatcggca
      421 gcactgctat tactggttaa agccaataat actacccact tatcactaaa atttaaaaaa
      481 ggttttaccg agtcggcacc catatatgga gcaacggtaa cggcatcggc ttgcatgtgt
      541 ttaaaaaatg cttgtgcata catggccgaa gtattgccta tatcgccacg tttggcatcg
      601 gcaattataa actgatcggg gtagttttgt ttaatataat caatagtttg ctgtaatgcc
      661 tgccagccac ttacaccata acattcgtaa aaagcggtat tgggtttata ggcaacacag
      721 taggtatgtg tagcatcaat aatggctttg ttaaactgaa aaatggcatc gggtaattgt
      781 ttgatatgcg caggaatttt ttcaatatca acatctaaac caatgcataa aaacgattgt
      841 ttttgttcga tgtttttaaa aagcgtttgt ttattcataa aggattataa ataaaaaatt
      901 aaacaatagc ttctttaagt ttttcggcat tttcggccaa ctgcaaggca tcaataaacg
      961 attgaatatc gccatccata aagccgggta agttaaaagt tgataagccg atgcggtggt
     1021 cggttacgcg actttgtgcg taattgtaag tgcggatttt tgccgacctg tcgccactgc
     1081 ttaccatcgt cttacgtttt ttagctattt catcctggcg tttttgtaat tcaacttcgt
     1141 aaatgcgcga gcgtaaaaca cctaatgctt tggcgtaatt ttttatctgc gatttttcgt
     1201 cctggcattg cacaacaata cctgtgggta tgtgtgtaag ccgtactgct gagtaggtgg
     1261 tattaaccga ttgaccacct gggcctgacg aacaaaaata atctttacgt atatcggctt
     1321 ctttaacttc tacatcaaac tcatcggctt cgggtaatac ggcaaccgtt gctgccgatg
     1381 tatgcacccg gccttgcgtt tcggtgaggg gtacgcgttg tacacgatgc acgcctgatt
     1441 cgtattttaa aataccataa ctgccttcgc ccaaaatatt aaagatgatt tctttataac
     1501 cacctgtact acctgcacta taatctacca gttctaattt ccagccgcgt gcttcgcaat
     1561 aacgggtgta catacggtat aagtcgcctg caaatatact tgcctcatcg ccaccggtac
     1621 cggctcttat ttcgaaaatg gcatttttgc tgtcttcggg gtcggcaggt atcaacaggt
     1681 atcttatctc atcttctaaa ttattgcgtt tctcctccag cgtttctaat tcggccttgg
     1741 ccatttcttt tatatcatcg tccttttcgg tatttagcaa atggtgtgct tcgtcatagt
     1801 ggtttaacac caacttatat tcctgcgttt tttcaactaa gccctttatt tctttgtatt
     1861 gcctgtttag tgctgcaaat cgtttcatgt ccgaaataac atcgggtgca cttaattggt
     1921 cttcaatttc cttaaacttt atttctattg cttcgagctt atctaacatt gcctgcgatt
     1981 ttttttttac gggggcgcaa acatactaaa attggccttt tatgacaggt aattgtttgt
     2041 ttggttgggt tagcaagctg ttaaataccc ggtatcagaa ttggcagcaa tgcatagcat
     2101 caacaaaaat tcattttgaa agattttttt aaaccatatt tgatcaaatt taaaatcatt
     2161 aaaaacatga actttttaaa attaaccgta gcagccgttg ttttaaatac tacagcgtat
     2221 gcccaaaccg aagccgaaat ggctgccaaa cctgttacca aaacagaagc cgttaaagag
     2281 cgcgctacgt acgaagagcg tgccaccaaa caagttaacc gtattagcga aattgtgcat
     2341 ttagatgata tgcagcatca aacaatcaaa gcattgtatt tagaatcgta tccgaagatt
     2401 aaggaagtgc acaaagcagc cggcaaggat accgcaagag ccgatgccga atcaaaaaaa
     2461 ataatgaaag agcgaagaaa aaatgtggaa gccttgctta acaaagaaca aaaaaacaaa
     2521 ctggaagcct acattaaagc gaacaaagat attgatgccg atgatgaaat taaagcaact
     2581 tcaaaatagc ccggaccctc tattgtttaa gccataaaat tttagccggt gttagcattc
     2641 gctaacaccg gctattttta ttttgtattt tgacttagta ggctttatgg ttaaaacgca
     2701 attttaaagt ctaatagata taaaaaaaat ttttttggac tctgatgcca atagctgtgt
     2761 acaaaccatt tcactaatca catcttacat ttcacctctt acctctcacc tctcaccgct
     2821 cactccttca caaacctgcc ataataaatt ttatcgtctt gctgcacttg caat
//
LOCUS       DAOMAA010000150         2866 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_28529_length_2866_cov_2.481679, whole genome shotgun sequence.
ACCESSION   DAOMAA010000150 DAOMAA010000000
VERSION     DAOMAA010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28529_length_2866_cov_2.481679"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1257)
                     /locus_tag="PLO59_02890"
     CDS             complement(<1..1257)
                     /locus_tag="PLO59_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016195330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HQV00075.1"
                     /translation="MTSDLVIDANDNEVTIALLEDKKLVELNSEKKDQNFAVGDVYLG
                     RVKKLLPGMNAAFIDIGYEKEGFLHYLDLGPQFESLNKFLKSTVAGKTTDALLTHFEN
                     ESDISKSGKINSVLTVGQSVLVQIAKEPISTKGHRLSTEISLAGRYVVMMPFSEGVTI
                     SAKIKNAEERNRLKRLAQSIKPKNFSLIVRTVAEGKMVAELDKDIQDLLSKWKQCHAL
                     LQQAQPPLKVLGEMDRTSTILRDLLNANFNNVHVNDAGLAQEIREYVKTIAPEHQELV
                     KLHKNKTPIFEQFGIDKQIITVFGKTVNLPGGAYLVIEHTEAMHVIDVNSGTRTKVDN
                     DGQEAHNLQVNLNAAREIARQLRLRDMGGIIVVDFIDLRNADNKKLLFECLRDEMKRD
                     RARHTILPPSKFGLIQITRERVRQVTE"
     gene            1825..>2866
                     /locus_tag="PLO59_02895"
     CDS             1825..>2866
                     /locus_tag="PLO59_02895"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HQV00076.1"
                     /translation="MTKVTFDNTNAQFFTALKQSVDAYFKQNRLKKTGNFKLYLKTLV
                     LIPAAIALYALVLFVPMSGWLAILLCALLGLDIAFIGFNVMHDACHGSYSTKKWVNYT
                     VGLSMNAIGSNAFIWKIKHNIIHHTYTNIDGVDDDIAKLPVIRLCESQKRLKMHKYQH
                     WYALFAYSLASLSWVLFTDFLKYFNQKIASTPINTFNTKEHLIFWASKVLYVLFYIAV
                     PIYLVGFVKFLIGFSVMHAVMGITIGIVFQLAHAVENTQFLDANDDKHQKLETGWAEH
                     QVATTSNFATKNKIVTWCLGGLNFQVEHHLFPRISHVHYPAINKIVIENCKKFGVTYN
                     AYPTMRAAIISHF"
BASE COUNT          817 a          545 c          525 g          979 t
ORIGIN      
        1 ttcggtaacc tgcctaaccc tttcgcgtgt aatttgaatt aaaccaaatt tactgggcgg
       61 taaaatggta tgccttgcgc ggtcgcgttt catttcatcg cgcaagcact caaacaaaag
      121 ttttttattg tcggcattac gcaggtcaat aaaatcaacc acaataatac cacccatatc
      181 gcgtaagcgc aactgacgtg caatttcgcg tgctgcattt aaattaacct gtaagttgtg
      241 cgcctcctgg ccatcattat caaccttggt acgtgtgccg ctatttacat caattacatg
      301 cattgcctca gtatgttcaa ttaccagata ggcaccgccc ggcaggttaa cagttttacc
      361 aaatacggtt ataatttgtt tgtcaatacc aaactgctcg aaaatgggcg ttttgttttt
      421 gtgcagcttt accaactcct gatgttcggg tgcaattgtt tttacatatt ctctgatttc
      481 ctgggcaagg ccggcatcgt taacatgtac gttattaaaa ttagcgttta gcaaatcgcg
      541 taaaatggtt gaagtgcgat ccatttcgcc cagcaccttt aatggtggtt gcgcttgttg
      601 taacagggca tggcattgtt tccatttcga taataaatcc tgaatatcct tatctaattc
      661 ggctaccatt ttaccttcgg caacggtgcg tactatcagg ctgaagtttt tgggcttaat
      721 gctttgcgcc aatcgtttga gccggttccg ttcttcggca ttttttattt ttgccgatat
      781 ggtaacgcct tcactaaaag gcatcattac cacatacctg cctgccagcg aaatttcggt
      841 agaaagcctg tgccctttgg tagaaatagg ttctttggca atttgaacca gcacactttg
      901 acctacggta agtacggaat taattttacc ggacttgcta atatctgatt cgttttcgaa
      961 atgcgttagc agtgcatccg tagttttacc cgcaaccgta ctttttaaaa acttattcag
     1021 cgattcgaat tgcggtccta aatcgaggta gtgcaaaaaa ccctcttttt cataaccaat
     1081 atcaataaaa gcagcattca tgccgggtag taattttttt accctaccta aatacacatc
     1141 gccaacggca aaattttgat cctttttttc gctattaagt tcaaccagtt ttttgtcttc
     1201 taatagcgca atggtgactt cattatcatt ggcgtctata actaaatcgc ttgtcacgca
     1261 gtgttttgtt gtataaatac gcaatcagcc ataactgtta aaactgaaaa agcagtttag
     1321 cgctatattt taaaagcatg ttgctgattt ataagcattt gttaaaaata aaaatacaga
     1381 ggttgtgtaa cacagcctct gtataaagtt ttaatattat atgaagtaca acgccttatt
     1441 atttaaggat tatgcactat attatttctt tttatgacgg tttttccgaa gacgcttttt
     1501 gcgcttatgg gttgccattt tatgtctttt cctttttttt ccgcttggca tgatgctaaa
     1561 tttttatgtg atgtttacgg tgataacctt atttaaaaga tgtaaacctt ttaaaatcgg
     1621 ggctgcaaat gtaaaattta tatcaactca aaaaagcatt attttaaaaa atgtttgctt
     1681 gtttttacag ctaatacttg tgtttaaagc gggcatcggt gttaataacc gcatcacact
     1741 aaataattta acctgtcgtt tggttcttta tcaattttgg cgctaaataa atggttacct
     1801 tgcgcccccc taaaaaacgt actcatgaca aaagtaactt ttgacaatac aaatgcccag
     1861 ttttttactg cacttaagca aagcgttgac gcgtatttta aacaaaaccg acttaaaaaa
     1921 acgggcaatt ttaagttgta tttaaaaacg ttggttctta tacccgcagc cattgcgctt
     1981 tatgctttgg tactgtttgt acccatgtcg gggtggttgg caattttgct gtgtgcatta
     2041 ttgggattag atatcgcttt tattggtttt aatgtgatgc atgatgcatg ccatggtagt
     2101 tactcaacta aaaaatgggt taactacaca gttggtttat ctatgaatgc aattggcagc
     2161 aatgccttta tatggaaaat aaaacataac atcatacacc atacttacac caatattgat
     2221 ggtgttgatg atgatatagc caaattacca gtaatacgtt tgtgcgaatc acaaaaacgt
     2281 ttaaaaatgc ataagtacca acattggtat gcgctttttg cctacagctt agcttcgtta
     2341 agttgggtat tgtttaccga ctttttaaaa tattttaatc agaaaattgc atcaacgcct
     2401 attaatactt tcaacacaaa agagcacctt attttttggg cgagtaaggt gttgtatgta
     2461 ttgttttaca tagcggtgcc catttattta gttggttttg taaagttttt aattggcttt
     2521 agcgtaatgc atgctgtaat ggggataacc attggtattg tttttcaatt agcacatgct
     2581 gtagaaaaca cccaattttt agatgccaat gatgataaac atcaaaaatt agaaaccggt
     2641 tgggccgaac atcaagtggc cactaccagt aactttgcta ctaaaaacaa aattgttacc
     2701 tggtgtttgg gcggcttaaa ctttcaggta gagcatcact tgtttccacg tattagtcat
     2761 gttcattatc cggccattaa taaaattgta attgaaaact gtaaaaaatt tggtgtcacg
     2821 tataatgcgt atcctactat gcgagctgct attatttcgc atttca
//
LOCUS       DAOMAA010000151         2864 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_28557_length_2864_cov_2.672837, whole genome shotgun sequence.
ACCESSION   DAOMAA010000151 DAOMAA010000000
VERSION     DAOMAA010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28557_length_2864_cov_2.672837"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="PLO59_02900"
     CDS             complement(<1..270)
                     /locus_tag="PLO59_02900"
                     /inference="COORDINATES: protein motif:HMM:NF025442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HQV00077.1"
                     /translation="MKKLPNSVKLILLVGMAAYALWQYTQQHNQPEPPLQNITYTKHA
                     RCRMQCRQIDETEVAEIIAQGHINKQKSNAADKPCPTIAYEGISHD"
     gene            complement(372..>2864)
                     /locus_tag="PLO59_02905"
     CDS             complement(372..>2864)
                     /locus_tag="PLO59_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00078.1"
                     /translation="NYPAYTGKTDEVIKNTGDLILPQGTQVTWHFNATQVETITLALN
                     DTTVTAQRKGENRFEFKQTFKSNTAYTIMPSNNYMLSKDSMHYTVSVTPDAYPTITVH
                     EQRDSILTKQIYFKGDINDDYGFSRLALAYRFIESGNDSIKHNTAINYQNIVVPKGMA
                     SHTFFYYLNVDDIKMGMGDAIEYYFEVWDNDGVNGAKSSKSFAQIIKAPTAAQLDAEK
                     ENANKALKQEMKEQIADAKKLQKETADALKKLTEKKTLDYDDKKKLENLLKQQKELQQ
                     KMESLKQQNENNQQKQNEFNKTDNERILEKQQQLQEMFEKTLSDEMKEKIKMMEQMLQ
                     ELDKDKMKQMLDQMKFDAKDLEKELDRNLALFKQLELEQKMDDAVRKLNDLAKEQEQL
                     AQKAEDKNSNANDINKQQDELNKKFDDIQKELKDAEQKNAELDNPMDLPKTDAEQKDV
                     ETQQQNAQQQLNKQDKKGASKSQKNAAKKMEDMAQKINQGMQQQQQEQAEEDMNALRI
                     LLENLIHFSFDQEKLMTQLKTIDVNNPQYLQLSQNQRKLKDDAKVLEDSLFELSKRVP
                     QIQSMVNQEIASINRNADAAIGHMQDRQVAIARSDQQYVMTSVNNLALMLSEALQQMQ
                     QQMQQQQSNASCSKPGKGKPSKGKPSASAMRKMQEELNKQMQKMKDGMKPGEGQKKAG
                     NGQKPGSGQSEQFAKMAAQQEALRQMLQDMENAKDGKGTSGTLGEMQKQMEETERDLV
                     NKRITEQTLKRQQDILTHLLESERAEREREQEEKRQSNTGNNLPNRNPSSFLEFQKQL
                     NGEKELLHVVPPALNTFYKNLVNSYFLQNNRP"
BASE COUNT          558 a          537 c          584 g         1185 t
ORIGIN      
        1 atcgtgcgat atgccttcgt atgcaatggt tggacaaggt ttgtcggctg cattgctttt
       61 ttgtttgttg atatggcctt gtgcaattat ttcggctact tcggtttcgt caatctgccg
      121 gcattgcata cggcaccgcg catgtttggt gtaagttata ttttgaagtg gtggttcggg
      181 ttgattatgc tgctgtgtat attgccataa tgcataagca gccatgccca caagcaaaat
      241 aagctttacg gaattgggca attttttcat acttcaatat aaactgggtt tggtttttgc
      301 aaatcaggtg caccatttgt aagtgcaaaa ttagtaacac ttactataca tgcaatggag
      361 cgctttacaa tttaaggcct gttattttgt aaaaagtaac tgtttaccaa gtttttataa
      421 aaggtgttta atgcaggcgg cactacatgc aacaattctt tttcgccatt gagttgcttt
      481 tgaaactcga gaaatgaaga agggtttcgg ttgggcaggt tgttgccggt attgctttgt
      541 cttttttctt cttgttcgcg ttcgcgctca gcacgttcgc tttcgagcag gtgtgttaat
      601 atatcttgtt ggcgttttaa tgtttgctcg gtaatgcgtt tgttaaccag gtcgcgttcg
      661 gtttcttcca tttgcttttg catttcgccc aatgtacccg atgtgccttt gccgtcttta
      721 gcgttttcca tgtcttgcag catttggcgc aatgcttctt gctgtgcagc catttttgca
      781 aattgctcgc tttgccccga tccgggtttt tggccattgc ctgctttttt ttgtccctca
      841 ccgggtttca tgccatcttt cattttttgc atctgcttat tcagttcttc ctgcattttg
      901 cgcatagccg atgccgatgg ttttccttta ctgggtttgc ctttgccggg cttgctgcac
      961 gatgcattgg attgctgttg ttgcatttgc tgttgcattt gttgcaatgc ttcgcttaac
     1021 atgagtgcca ggttgtttac cgatgtcatt acatattgtt ggtcgctgcg ggctatggct
     1081 acctgcctgt cttgcatgtg accaatagca gcatcggcat tgcggtttat cgatgcaatt
     1141 tcctgattta ccatgctttg tatttgtggt acgcgcttgc ttagttcaaa caacgagtct
     1201 tctaatactt tggcatcgtc tttaagtttt cgttggtttt gcgatagttg taaatactgt
     1261 gggttgttta catctatggt ttttaattgc gtcattaatt tttcctgatc gaaggagaaa
     1321 tgaatcaggt tttctaaaag tatgcgtaag gcattcatat cttcttcggc ttgctcctgt
     1381 tgttgttgct gcatgccttg atttattttt tgcgccatat cttccatttt ttttgctgca
     1441 tttttttgcg atttggaagc acctttttta tcctgtttat ttaattgttg ttgtgcgttt
     1501 tgttgttgtg tttccacatc tttttgctcg gcatcggttt taggtaaatc catcggattg
     1561 tctaattccg catttttttg ttcggcatct ttcagttctt tttgtatgtc gtcaaacttt
     1621 ttattcagct catcttgttg tttattaatg tcgttggcat tgctgttttt gtcttcggct
     1681 ttttgtgcta attgttcttg ttcttttgcc aggtcgttga gtttgcgtac ggcatcatcc
     1741 atcttttgtt caagttctaa ctgtttaaac aaagccaggt ttctgtctaa ttctttttct
     1801 aagtctttgg catcaaactt catttgatcg agcatttgtt tcattttatc cttatccagt
     1861 tcttgcaaca tttgctccat cattttaatt ttttctttca tttcatcgct taatgttttt
     1921 tcaaacatct cctgcagttg ttgctgtttt tcgagtatgc gttcgttatc ggttttgtta
     1981 aactcatttt gtttttgctg attgttttcg ttttgctgtt taagcgattc cattttttgc
     2041 tgtaactctt tttgttgttt caacaaattt tccagtttct ttttatcgtc atagtcaagc
     2101 gtttttttct ctgttagttt tttcaaagca tcggctgttt ctttttgaag tttttttgca
     2161 tcggctattt gttctttcat ttcttgcttc aacgctttgt tggcattttc tttttcggca
     2221 tctaattgtg cagcagttgg tgcttttatt atttgtgcaa agcttttgct gcttttcgct
     2281 ccattaacgc catcattatc ccaaacttca aaatagtatt ctatagcatc gcccataccc
     2341 attttaatat catcaacatt taggtaataa aaaaaagtat gcgatgccat acccttgggt
     2401 actacaatgt tttgatagtt aatagctgta ttgtgtttaa tactatcgtt gcccgattcg
     2461 ataaagcgat aagctagtgc caaacggcta aagccgtagt cgtcattaat atcaccttta
     2521 aaataaattt gtttggtaag tattgagtcg cgttgttcgt gtacggtaat ggttggataa
     2581 gcatcgggtg taacacttac tgtatagtgc atgctgtctt tgcttagcat gtaattgtta
     2641 gatggcatta ttgtatatgc cgtgttgctt ttaaaggtct gcttaaattc gaacctgttt
     2701 tcgccctttc gttgtgcagt tacagtagta tcatttaagg ccagggtaat ggtttcgact
     2761 tgggttgcat taaaatgcca ggttacttgc gtgccttgcg gtagtattaa atcgcctgtg
     2821 tttttaataa cttcatctgt tttgcctgta tatgcaggat agtt
//
LOCUS       DAOMAA010000152         2862 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_28606_length_2862_cov_2.947631, whole genome shotgun sequence.
ACCESSION   DAOMAA010000152 DAOMAA010000000
VERSION     DAOMAA010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28606_length_2862_cov_2.947631"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2862)
                     /locus_tag="PLO59_02910"
     CDS             complement(<1..>2862)
                     /locus_tag="PLO59_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00079.1"
                     /translation="VTATASATTICTGSSLTLTANLTGGNSNNTKSFIWVPSENITNP
                     NQAVCTAKPTQTTTYTVYATDGNDYTAQASVTVTVGPDAEAGDDQYIVSGTSVQLTAA
                     NIKVGVSYKWYLNGSLLGNISVPVTPLVKSTYMLVARQNQNTCSDTDYVTITPLITCA
                     SALDTFLTIPPNFTVKQFVNRMKIYSSLTGNAIANDTLLSNLSLPIYFNGPFIIDNHY
                     TFVNCTNMYFSEGAYILQNTDNKNLSLYNCQLKAAPCTQKMWRGIVMQEYGEKLTLNN
                     CLIEDAINGVEATNNTRIFATNTTFKRCYVGIYFHDYDEVDLGGFIVGCKFVGTKCTL
                     EPYLNQTRLAGIYCNNAYMIEIGSNTSASNIFNKSLYGIYSINTSVKIYNSIFSKMRQ
                     QEGAFPNTGTCIYMKNSAGTGNINNPLHLWPMYELFAGDSINNYGNEFKNSNKGILSD
                     NCAVNATGNLFDNLGVAFTANGCVYKELVLRKNTVINTDNGFSLIDNKWATSDITDNN
                     ITIRAVTSNSSGTYGLRIADAAGFSSILNIENNHFTSRGIYAMQITNNVLGNIESNSI
                     YMDNTAPLTAYGIKLEACDSMKVNCNNIYGGTLANYGNQRAVSLSFTPNSYLECNETH
                     KTGIGFEFLADCNFAYLKNNTFRRHKIGISVGDIGITGGIIGPQPPVSNLRPLGNIFA
                     GEYTIATGVGKGKGYFVTAATQSVNSKLAGGIGSLKQFIVYDGDPIQTPYPNIKIGTA
                     STALNPQKNTKIPLDCNANCSMPDAANMRTTEIEKPTISDYSNLKNQLVQTFNDETND
                     VIQQHLKIDLIRTASDFDLGNGSNSHQLKAFYNQMQQTNIGFIANISKAYSKLNGCNN
                     IEENKRLILTAAQLSAAITPDNIYEENSKQIESIKTKYLLSMQSDMDAFELAELFGIA
                     SQCPYVGGPAVFDARALYVKYYPNIVFDDHLICKTGTMH"
BASE COUNT          846 a          502 c          530 g          984 t
ORIGIN      
        1 ttatgcatgg tgccggtttt acaaattaaa tggtcatcaa aaacaatatt aggatagtat
       61 ttaacataca atgcacgcgc atcaaataca gccggcccac caacataagg gcattgtgat
      121 gcaataccaa aaagttccgc taattcaaat gcatccatat cgctttgcat agacaacagg
      181 tacttggttt ttatgctttc tatttgtttg gaattttctt cataaatatt gtcaggcgta
      241 attgctgccg aaagctgggc tgcagttagt ataagtcgct tgttttcttc aatattgtta
      301 cagccgttta actttgaata ggctttcgaa atattagcaa taaagccaat attagtttgc
      361 tgcatctgat tgtaaaaagc tttcaattga tgcgaattgg atccattacc taaatcaaaa
      421 tcagaagcag ttctaattaa atcaattttc agatgctgtt gtatcacatc atttgtttcg
      481 tcattaaaag tttgtaccaa ttgattttta agatttgaat aatcggaaat agttggtttt
      541 tcgatttctg tggtgcgcat gttagcagcg tcaggcatac tacaattagc attacaatca
      601 agagggattt tagtgttttt ttgtgggttt aatgcagttg atgccgtacc aatttttata
      661 tttggataag gtgtttgtat tgggtcacca tcgtaaacaa taaattgttt taatgaacca
      721 attcctccgg ctaatttaga attaaccgat tgtgttgcgg cagttacaaa atatcctttg
      781 cctttgccaa cgcctgttgc aattgtatat tctccggcaa agatattacc aagtggacgc
      841 aaattagaaa ccgggggttg cgggccaata ataccaccgg taattccgat atcacctact
      901 gaaatgccga tcttatgtct tctgaatgta ttgtttttta aataagcgaa attgcaatcg
      961 gctaaaaact caaaaccaat tcctgtctta tgtgtttcgt tacattctaa gtatgaatta
     1021 ggtgtaaagc ttaacgaaac cgcacgttgg ttaccatagt tggccaatgt gcctccataa
     1081 atgttattgc agttaacttt catcgaatcg catgcttcta atttgatacc ataagcagtt
     1141 aagggtgcgg tattatccat atatatcgaa ttgctttcga tattgcctaa tacgttattg
     1201 gtaatttgca tggcataaat accacggctt gtaaaatgat tgttttcaat atttaaaatt
     1261 gatgaaaaac cagctgcatc agcaatacgt aaaccatagg ttccgctgct attagatgta
     1321 acggctctta tggttatgtt gttgtcagtt atatcgctgg ttgcccattt attgtctata
     1381 agcgaaaagc cattgtcggt gttgattacc gtattttttc taagtactaa ttctttataa
     1441 acacatccat tagcggtaaa agctacacct aaattgtcaa ataggtttcc tgttgcatta
     1501 actgcacagt tatcgctcaa tatacctttg tttgagtttt taaattcgtt tccatagtta
     1561 ttaattgagt cccctgcaaa caattcatac atgggccata agtgtaaggg gttgtttata
     1621 ttaccggtgc cggcactgtt tttcatgtaa atgcaagtac cggtattcgg aaaagcacct
     1681 tcctgttgcc tcattttaga gaatatgctg ttgtaaatct ttacactggt gtttattgaa
     1741 taaataccat aaagtgattt gttaaaaata ttggaagcgc tagtgttgct tccaatttct
     1801 atcatatatg cattattaca atatattccc gctaaccgag tttggtttaa gtagggctct
     1861 aaagtgcact ttgtgccaac aaacttacac cctacaatga atccccctaa atctacctcg
     1921 tcataatcat gaaaataaat gccaacgtaa cagcgcttaa atgtggtgtt ggttgcaaaa
     1981 atgcgtgtat tgtttgttgc ttcaacacca tttatggcat cttcaattaa acaattattt
     2041 aaagttagtt tctcgccata ttcttgcatt acaatgccac gccacatttt ttgtgtacag
     2101 ggagctgcct ttagttgaca attataaagc gataaatttt tattgtctgt attttgaagt
     2161 atgtaagcac cttcgctaaa atacatgttt gtacaattaa caaaagtata gtgattgtca
     2221 ataataaatg ggccattgaa atatatgggc aatgataaat tgcttaacaa agtatcattg
     2281 gcaatggcat taccggttaa gctgctgtaa atcttcatgc gatttacaaa ttgtttaacc
     2341 gtaaaatttg gtggtatggt taaaaatgta tctaaggctg atgcacatgt aattaatggg
     2401 gtaattgtaa cataatcagt atcagagcaa gtattttgat tttgacgtgc taccaacatg
     2461 tatgtgcttt taactaaagg tgtaacaggt actgaaatgt ttcctaataa actgccattt
     2521 aagtaccatt tataactaac acctaccttg atatttgcag cagttaattg tacagaagtg
     2581 ccactcacaa tgtactgatc gtcaccggct tcggcatcgg gtcctactgt aactgtaacc
     2641 gatgcctgtg ctgtatagtc gtttccatcg gtagcataaa ccgtgtaggt tgttgtttgt
     2701 gtaggctttg ctgtacaaac tgcctgattg ggattggtaa tattttcgga tggtacccaa
     2761 ataaacgatt tggtattgtt actattacca cccgttaaat ttgctgttaa tgttaaggag
     2821 ctacctgtac aaatagtagt agccgatgct gttgcagtta ca
//
LOCUS       DAOMAA010000153         2851 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_28803_length_2851_cov_3.873391, whole genome shotgun sequence.
ACCESSION   DAOMAA010000153 DAOMAA010000000
VERSION     DAOMAA010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28803_length_2851_cov_3.873391"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            227..1423
                     /locus_tag="PLO59_02915"
     CDS             227..1423
                     /locus_tag="PLO59_02915"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQV00080.1"
                     /translation="MTQPYIKNEKAVLFLLAALQFTNIMDFMIMMPLSVHIMPAFNIS
                     PQQFSFIVSAYAFAAFGSSIAASFFVDKFDRKSLLLFVYTGFLIGTFACAWAPTFYFL
                     IFARIFAGLFGGLIAAQVLAIVGDYIPFERRGKAMGVLFAGFSLASVAGVPVGIYLAD
                     TFQWHIPFIMVGAMGVILLPTIFFLLPQLKIHLEDKNEKPHVAKIIVNDTNLQIALLM
                     MFTLVLSHFVTIPFLAQYIEKNVGFTKAQIAWVYMVGGLSTLIFSPVIGRLADKYGKY
                     KVFAYLVIGSWFPVFVLTNLQQVSIVWVLLVAALFFIFGAGRYVPAQALITSVVKPQY
                     RGGFMNINASSQNLATALAALISGFIVTTDENGKLLHYNWVGFFAIAVSIICITVARK
                     LIVKAD"
     gene            1450..>2851
                     /gene="argS"
                     /locus_tag="PLO59_02920"
     CDS             1450..>2851
                     /gene="argS"
                     /locus_tag="PLO59_02920"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013631841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HQV00081.1"
                     /translation="MIFSEEIKGLITQAIYQLYNVSIETSQILIEPTKKEFEGDVTFV
                     VFPYLKISKKSPVETAKQIGEIVVQSSGLISSFQAVAGFLNLSINNKVYVDAFLNPAL
                     KTSSIKPNPVLVEYSSPNTNKPLHLGHIRNILLGFSLSRILAADGNNIKKVNIVNDRG
                     VHICKSMLAWQLFGNNETPESTGIKGDHLVGKYYVVFDAKYREQVTEMEARGISKDVA
                     EKQAPLMLQVQEMLRKWETGDEAVLSLWQTMNGWVYDGFDITYSKLGVDFDEIYYESN
                     TYLLGKEIVAAGLNKGVFFKKEDGSVWVDLTDEGLDLKLLQRSDGTSVYITQDLGTAK
                     LRFDAYNFDKMIYVVGNEQDYHFKVLRLVFKKLGYPFWDKLYHMSYNMVDLPSGKMKS
                     REGTVVDADDLIAQTIANAETLTRSMGKLDVYDDAHAQQLYHTIGMGALKYYILKVDP
                     EKRMLFNPAESIDINGN"
BASE COUNT          876 a          453 c          583 g          939 t
ORIGIN      
        1 aattatacat cgtaaagatt ataaagatac ctatcaaaaa gtagttgcca gttacggaag
       61 tgtatttttt aaaaatgata aaagcatttc acaaacactt tacgatcagc aaataaaaac
      121 tgctaaagca caattaggta ataactaatg attattgatt attgattaaa ttaaaatttg
      181 ttatcaatct gtttacatta ggttgagttt gtactttctc taaaacatga cgcaaccata
      241 tattaaaaac gaaaaggcag ttttattttt attagcggca ctacaattta caaacatcat
      301 ggattttatg atcatgatgc cgttaagcgt acacataatg cccgcattta atatcagtcc
      361 gcagcaattt agctttatag tttcggccta tgcatttgcc gcatttggca gcagtatagc
      421 agccagcttt tttgtcgaca agttcgatcg gaaatcgttg ctgctatttg tttacacggg
      481 ctttttaatt ggaacgtttg cttgtgcatg ggcacccact ttttacttct tgatttttgc
      541 tcgcattttt gccggcttgt ttggcggcct tattgcagcc caggtattgg ctattgttgg
      601 cgattatata ccgtttgaaa gacgcggtaa agccatggga gttttgtttg caggtttttc
      661 tttagcttcg gttgcaggcg tgcctgtagg tatttattta gccgatactt ttcaatggca
      721 tattcctttt ataatggtag gtgcaatggg cgttatttta ttaccaacca ttttttttct
      781 tttaccgcag cttaaaattc acttagagga taaaaatgaa aagccgcatg tagctaagat
      841 tattgtaaac gacaccaact tacaaattgc tttgcttatg atgtttacat tggtattaag
      901 tcattttgta accatcccgt ttttagccca gtatatcgaa aaaaatgtag gttttacaaa
      961 agcccaaatt gcctgggtat atatggtagg tggattaagt accttaattt tttcgcctgt
     1021 tattggtcgc cttgccgata agtatggcaa gtataaagtg tttgcctatc tggtaattgg
     1081 ctcttggttt ccggtatttg ttttaaccaa cttacagcaa gtgtctattg tatgggtgtt
     1141 attagtagct gctttgtttt ttatttttgg tgccgggcgt tacgtaccgg cacaagccct
     1201 tattaccagc gtagttaaac cacaataccg gggtggcttt atgaatatta atgcatcgag
     1261 tcaaaatctg gcaacagcct tagctgcatt aatttcaggc tttatagtta caaccgatga
     1321 aaacggcaaa ttgcttcatt acaactgggt aggatttttt gctattgctg taagcattat
     1381 ttgtattacc gttgcccgta aattaattgt taaagccgac taaaatttaa atgtaatctt
     1441 actgcaacta tgattttttc tgaagaaata aaaggtttga taacacaggc catataccaa
     1501 ctttataacg tatcaatcga aacgtctcaa attttgattg agccaactaa aaaagaattt
     1561 gagggcgatg taacttttgt tgtttttccg tatttaaaaa tatctaaaaa aagccctgta
     1621 gaaactgcaa aacaaatagg agaaattgtt gttcaatctt ccggattaat aagcagtttt
     1681 caggcagtgg ccggattttt aaatttatct attaataaca aagtttatgt tgatgcattt
     1741 ttaaatcccg cgctcaaaac atcttcaata aaaccaaacc ctgttttagt tgagtacagt
     1801 tcgcccaata caaacaaacc attgcactta gggcatatac gcaatatttt attaggcttt
     1861 tcattgtcgc gcattttagc tgccgatgga aacaacatta aaaaagtaaa tattgtaaat
     1921 gacaggggtg tgcatatttg taaatcaatg ttggcatggc agctatttgg taataacgaa
     1981 acaccggaaa gtaccggtat taaaggcgat catttagtgg gcaaatatta tgtggttttt
     2041 gatgctaagt atagggagca ggttaccgaa atggaagccc ggggcatatc aaaggatgta
     2101 gccgaaaagc aagcaccatt aatgctgcaa gtgcaggaaa tgttgcgcaa atgggaaaca
     2161 ggtgatgaag ctgtgcttag tttatggcaa actatgaatg gttgggttta tgatggattt
     2221 gatattactt acagtaaatt aggtgttgat tttgatgaaa tatattatga gagtaatacc
     2281 tatttgttgg gaaaagaaat tgtagcagcg ggtttaaaca agggtgtgtt ttttaagaaa
     2341 gaagatggtt cggtatgggt tgatttaacg gatgaaggat tagacttaaa gctattgcag
     2401 cgtagcgatg gaacatcggt ttatattacg caagatttag gcacggcaaa gttgcgcttt
     2461 gatgcttaca acttcgataa aatgatttat gttgtaggta atgagcagga ttatcatttc
     2521 aaagtattgc gcctggtctt taaaaaatta ggttatccgt tttgggataa attatatcac
     2581 atgtcataca acatggttga tttaccaagt ggtaaaatga aatcgcgtga aggtacggtg
     2641 gttgatgccg atgatttaat agcacaaaca attgccaatg ccgaaacact aacgcgcagc
     2701 atgggtaagt tagatgtata tgacgatgcg catgcacaac aactgtatca tactataggt
     2761 atgggtgcat taaaatatta catattaaaa gttgaccctg aaaaacgaat gctgtttaac
     2821 ccggccgaga gcattgatat taacggcaat a
//
LOCUS       DAOMAA010000154         2848 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_28862_length_2848_cov_2.574293, whole genome shotgun sequence.
ACCESSION   DAOMAA010000154 DAOMAA010000000
VERSION     DAOMAA010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28862_length_2848_cov_2.574293"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="PLO59_02925"
     CDS             complement(<1..661)
                     /locus_tag="PLO59_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013664976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQV00082.1"
                     /translation="MIFDVVIIGSGPGGYVSAIRCAQLGFKTAIVERYETLGGTCLNV
                     GCIPSKALLDSSEHFYNAQHTFVQHGIEATGLKVNLQQMISRKNGVVSQTVDGINYLM
                     KKNKITVLHGVGAFKSTTEVTVTGNDGTIKNITAKYIIVATGSKPALPKGITTDSKRI
                     ITSTEALQLTEIPKQLVIIGGGVIGLELGSVYARLGAKVKVIEYTDGIIPAMDKALGK
                     EL"
     gene            complement(676..2271)
                     /gene="lnt"
                     /locus_tag="PLO59_02930"
     CDS             complement(676..2271)
                     /gene="lnt"
                     /locus_tag="PLO59_02930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HQV00083.1"
                     /translation="MISFLKSHLALSISFAALQWLAWPASGYAPILFIAWLPLLQLQY
                     RYSISHITTKQFFGYTYLAFMFFNIAVTWWVYYASLFGVVMAVLLNALFMTWAFLLFG
                     FIQKKCNHILSWLVLIPVWVTWEWLHLDWDLSWPWLTMGNGLAKMHTLIQWYSYTGVF
                     GGTTWIILINILLFMAIHNNKSRKRIVTITSIAAACFIIPATVSNYQYQHYTESNSPV
                     NVVVLQPNIDPYNEKFGGLTGQQQLEKMMQLTQGYIDSTTDYLIGPETSIPYAIWENE
                     LPQNPQIVYLKNTLHNYPKLTLITGAATDKLYPDSKAISATARKFRDTTLYYDTYNTA
                     IQIDSTSVMALYHKSKLVPGVEKMPFPQLFKPIEKLAINLGGSSGSLGVDTAPSVFIN
                     QSKLASMAVAPIICYESIYGAYVAKYVQKGAAWLCIITNDGWWYDTPGYKQHILFGRL
                     RAIEMRRSIARSANTGVSAIINQRGDILNHTKFWEPAVIKGTLQANNYITFYCRHGDY
                     LAHISACLTVLLLTCALILKFKN"
     gene            complement(2296..>2848)
                     /locus_tag="PLO59_02935"
     CDS             complement(2296..>2848)
                     /locus_tag="PLO59_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00084.1"
                     /translation="VPLITLLLIITYIIFICLGHRQHLYTHTLQYLPNIACGIWLAYA
                     MHFKFEWFGKIARWRLWVLILVPLLCYINAAPSGVMQILLLKALSLMVFGSVVAYLAR
                     TTFSSNQTFIYWGKRSYGLYIWHGVTLTIYAFVCKSNTFTGWQIAVAFLLCLLATHGL
                     AWLCYRYIEMPFLRLKKHLPFLN"
BASE COUNT          901 a          584 c          535 g          828 t
ORIGIN      
        1 caagctcctt gcccaaagct ttgtccatgg caggaatgat accatctgta tattcaataa
       61 ccttaacctt tgcacccaaa cgtgcataaa cgcttcccag ctcaagacca atcacacctc
      121 cgccaataat taccaactgt tttggtattt cggttaattg caatgcttca gtactggtaa
      181 taatacgttt gctatcggtt gtaatacctt taggcaaggc cggttttgag cctgtagcaa
      241 caataatgta tttggctgtt atgtttttta tggtgccatc gttaccggtt accgtaactt
      301 cagtagttga tttaaatgca ccaacaccat gcaatacggt aattttattt tttttcatca
      361 ggtaatttat gccatcaacc gtttggctta ccacaccatt tttacggctt atcatttgtt
      421 gcaggttaac ttttaaccct gttgcttcaa taccatgttg cacaaaagtg tgttgggcat
      481 tataaaagtg ttctgacgag tcgagcaagg ctttgctggg aatgcagcct acatttaaac
      541 aagtgccgcc taaggtttca tacctttcaa caatagctgt tttaaatcct aattgcgcac
      601 atcgaatggc gcttacatat ccacctggcc cagagccaat aatcactaca tcgaatatca
      661 taaaaacaaa aaggcttaat ttttaaactt taaaataagg gcgcaagtta aaagcaaaac
      721 ggttaagcat gcacttatat gtgctaaata atcgccatgc ctgcaataaa aagttatata
      781 attgtttgcc tgcaatgtgc ctttaataac agcgggttcc caaaatttgg tatgatttaa
      841 aatatcgcca cgctgattaa ttatagccga aacgcctgta ttagccgaac gtgctatgct
      901 tctgcgcatt tcgatggcac gcaagcgccc aaataaaatg tgctgcttat agccgggtgt
      961 atcataccac caaccatcgt tggtaataat acaaagccag gcagcccctt tttgtacata
     1021 cttggcaaca taggcgccat atattgattc ataacaaata ataggcgcta cggccatgct
     1081 tgccagttta ctttggttga taaaaaccga gggggccgta tctacaccca aactacctga
     1141 cgagccgcct aaattgattg ccaatttttc aatcggttta aacagttgag gaaatggcat
     1201 tttctcaaca ccgggtacta atttcgattt atgatagagg gccataaccg atgtactgtc
     1261 tatttgtatg gctgtattgt atgtatcgta atacaaagtg gtatctctaa acttacgcgc
     1321 agtagccgat atggctttgc tgtcagggta taatttgtcg gtagcggcac cggtaattaa
     1381 tgttagtttc ggatagttat gtaacgtgtt tttcaaataa actatttgcg ggttttgcgg
     1441 cagttcattt tcccaaatgg cataaggtat agaagtttcg gggcctatta aataatcggt
     1501 agtactgtca atgtagccct gtgtgagttg catcatcttt tctaattgct gttgcccggt
     1561 aaggccacca aatttttcat tatatggatc aatgttgggt tgcaacacta ccacattaac
     1621 agggctgtta ctttccgtat aatgttgata ttgatagttt gaaactgtgg caggtataat
     1681 aaaacatgca gcggcaatgg atgtaattgt cacaatacgt tttcttgatt tattgttatg
     1741 aatagccata aacaacaaaa tgtttattag tattatccat gtagtgcctc caaaaacacc
     1801 tgtgtagcta taccactgaa ttagggtatg cattttagcc aaaccgttgc ccatggtaag
     1861 ccagggccag gataggtccc aatctaaatg cagccactcc caggtaaccc aaaccggaat
     1921 tagtaccaac caggataaaa tatggttgca ttttttttga ataaaaccaa aaagtaaaaa
     1981 tgcccaggtc ataaaaagtg cattaagcaa aacggccata accacaccaa acaaacttgc
     2041 ataatacacc caccaggtta cagcaatatt aaaaaacata aaggccagat atgtgtaacc
     2101 aaaaaactgt ttggttgtaa tatgactgat gctgtaccta tattgtaact gtaacaatgg
     2161 cagccaggca ataaacagga tcggtgcata gccgctagcc ggccaagcca gccattgcaa
     2221 agcggcaaat gaaatgctta aagcaaggtg cgattttaaa aatgaaatca tgctgcaaag
     2281 ttatattgcc ttacattagt ttaaaaaggg caggtgtttt tttaatctta aaaaaggcat
     2341 ttcaatatac cgatagcata gccaggccaa accatgtgtt gctaaaaggc aaagcaaaaa
     2401 tgctactgca atttgccacc ctgtaaacgt attggattta cacacaaatg catatatagt
     2461 aagtgttacg ccatgccata tatataagcc ataactgcgt ttgccccagt atataaatgt
     2521 ttggttgctt gaaaatgtag tccgtgctaa atatgctaca acactaccaa acaccataag
     2581 gctgagtgcc ttaagcaaca agatttgcat tacacccgaa ggtgcagcat ttatataaca
     2641 caacaacggt actaatatta gtacccataa acgccacctt gctattttac caaaccattc
     2701 aaacttaaaa tgcatggcat aagccagcca aataccacaa gctatattag gcaaatactg
     2761 caaggtatgt gtgtaaaggt gttgcctgtg gcctaagcat ataaaaatga tataagttat
     2821 tatcagcaac aaagttatca aaggcact
//
LOCUS       DAOMAA010000155         2845 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_28917_length_2845_cov_3.040860, whole genome shotgun sequence.
ACCESSION   DAOMAA010000155 DAOMAA010000000
VERSION     DAOMAA010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28917_length_2845_cov_3.040860"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..460)
                     /locus_tag="PLO59_02940"
     CDS             complement(38..460)
                     /locus_tag="PLO59_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013870243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HQV00085.1"
                     /translation="MDADKQAMHIAQTMMQNDAYSQWLGMELLEVKQACCKVKMTVTS
                     NMTNGFGIAHGGITYALADSAFAFACNSHGRKSVSVETSISHTAPVQTGDELIAVATE
                     LSLSNKIGVYQIVITCNNKTVALFKGTCYRTSKHWALS"
     gene            complement(468..1661)
                     /locus_tag="PLO59_02945"
     CDS             complement(468..1661)
                     /locus_tag="PLO59_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020404024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HQV00086.1"
                     /translation="MFKSNCTIGIVGGGAMGAGIAQVAAIAGHTVFVYDVCEATLQKA
                     VAGWQNSLNKLAEKNKLSKQHAIEILNRLLIKNNLADLSESQLIIEAVVEDIQVKQTL
                     FTQLEKVVAPDCVLASNTSSLSIASIASACNNPQRVLGIHFFNPAILMPLVEIIPGIA
                     TDENCWQACRTLINSWGKTTVITKDTPGFIVNRIARSYYGESIRIYEEGIADFATIDW
                     ALKTFGGFKMGPFELMDFIGNDVNYKVTETVWQQFFYEPRFKPSLTQKRLYEAHFFGR
                     KSGRGYYNYQDNAAMPLPNKNETLGKEICNRVLCMLINEAIDAYYLKLATKEDLEIAM
                     EKGVNYPKGLLMWADEIGLPHVLHTLQSLYNDYGEDRYRPSILLKRMVAANQNFYEKS
                     FWHKK"
     gene            complement(1670..2098)
                     /gene="rpiB"
                     /locus_tag="PLO59_02950"
     CDS             complement(1670..2098)
                     /gene="rpiB"
                     /locus_tag="PLO59_02950"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010228189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HQV00087.1"
                     /translation="MQLYIGADHAGFQLKETIKSHLVNKGYTVIDKGTFSETSTDYPP
                     YAHEVAIQVANQNAMGILICGSANGVCITANKHAGVRAALCWQTDVATLARQHNDANI
                     ICIPARFVTNTEALSMVDVFLNTPFEGGRHANRVAKINIQ"
     gene            2667..>2845
                     /gene="nadA"
                     /locus_tag="PLO59_02955"
     CDS             2667..>2845
                     /gene="nadA"
                     /locus_tag="PLO59_02955"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013634043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HQV00088.1"
                     /translation="MTHFEQVDKRRLFDGMIDPSLDLFTEINRLKKEKNAVLLAHYYQ
                     DADIQDIADYIGDSLG"
BASE COUNT          841 a          503 c          486 g         1015 t
ORIGIN      
        1 taagtatgtt tttaataata tttaaggtta aactgtttta gctaagcgcc caatgtttcg
       61 atgtacgata gcaggtgcct ttaaataatg ccaccgtttt attgttgcat gtaataacaa
      121 tttgataaac gccaattttg ttacttaaac tcaattctgt ggcaacagca attaattcat
      181 cgcctgtttg taccggagct gtatgcgata tggatgtttc taccgataca cttttgcggc
      241 catgcgagtt gcaggcaaaa gcaaaagcac tatcggccag tgcataggta atgccaccat
      301 gtgctatacc aaaaccattt gtcatgtttg atgttactgt catcttaact ttacagcatg
      361 cttgtttcac ttctaataat tccataccca accattggct ataagcatca ttctgcatca
      421 tggtttgggc aatgtgcata gcttgtttat ctgcatccat ttgattttta ttttttatgc
      481 caaaatgatt tttcgtaaaa attttgattg gctgctacca tccgttttag caatattgaa
      541 gggcggtatc ggtcttcgcc ataatcgtta tataatgatt gtaatgtatg aagtacatgt
      601 ggcaaaccaa tttcatcggc ccacattaat aagcctttcg ggtaattaac gcctttttcc
      661 atggctattt ctaaatcttc tttagtggct aatttcagat agtaagcatc aatagcttcg
      721 tttataagca tacataaaac gcggttgcaa atttctttac ctaatgtttc gtttttattt
      781 ggtagcggca ttgctgcatt atcttgatag ttatagtaac cgcggcccga tttacgacca
      841 aagaaatgtg cttcatacaa acgtttttgt gttagtgaag gtttaaagcg tggctcgtaa
      901 aaaaattgtt gccacaccgt ttcggtaact ttataattaa catcgtttcc tataaagtcc
      961 atcaattcaa acggacccat tttaaaacca ccaaaagttt ttaaagccca atctatagtt
     1021 gcaaaatcgg ctatgccttc ttcataaatt ctaatgcttt cgccataata gctgcgtgct
     1081 atgcggttaa ctataaagcc cggtgtatct tttgtaataa ccgtggtttt gccccaacta
     1141 tttattaaag tacgacatgc ctgccagcag ttttcatcag tagcaatgcc gggtattatt
     1201 tcaaccaaag gcataagtat tgccgggtta aaaaaatgta tgcccaatac gcgttgtggg
     1261 ttgttgcatg ccgatgcaat agaagcaata cttaatgaag atgtgttaga ggccagtaca
     1321 caatcgggtg ctactacttt ttcgagttgg gtaaaaagtg tttgtttcac ctgaatatct
     1381 tcaacaactg cttctataat cagttggctt tcgcttaaat cagctaaatt gttttttata
     1441 agcaagcggt ttaatatttc gatagcatgt tgtttagaca gtttattttt ttcggccagt
     1501 ttgtttaatg agttttgcca acctgctacc gctttttgta atgtagcttc acatacatca
     1561 taaacaaaaa cagtatggcc ggctatggct gcaacctgcg ctataccggc acccatggca
     1621 cctccaccaa caataccaat ggtacagtta cttttaaaca tatatctatc tattgtatat
     1681 ttattttagc tactctattt gcatgtctgc ctccttcgaa cggtgtattt agaaatacat
     1741 caaccatact taatgcctct gtatttgtta caaaacgtgc aggaatacaa ataatgttgg
     1801 catcgttgtg ttgccttgcc aatgttgcta catcagtttg ccagcacaat gcagctctta
     1861 caccggcatg tttgtttgca gttatgcaaa cgccattggc actaccacaa attaaaatac
     1921 ccattgcatt ttgattggct acttgtatag ctacttcgtg ggcgtaaggc ggatagtctg
     1981 ttgatgtttc agaaaaagta cccttatcaa taacagtata acctttattt actaaatggc
     2041 tttttatggt ttcttttaat tgaaaaccgg catggtcggc acctatatac agttgcatac
     2101 gtttttgttt tgtgcaaaga aacggttgtt ttcttaaacc tactacattt ttacgtgtat
     2161 agcttgcagc gctaattctg tgcggtgttt tattaaacct tttaaacagg ttaatttttt
     2221 ataatctaaa gtttatagtg tttacaaggc ttttaaaatt tggcttttaa acaaccattt
     2281 cgtgtatatt ttttttttgc gttaataact ttgggcaatt ataatttgga atgttgatag
     2341 gtgtgtttaa gtaaaacaga tttgttagtt tacatatttt aaaaaataaa attctaaaaa
     2401 tattacacag ttcaatcaac actttttttt aagtcatcaa caaaaacttg tgttgttact
     2461 taaccattca caagttgtga ataaggttta aaatcctttt actaataagg gtttgtaatg
     2521 tgtataaact gtgaaatact ttagcttcga aactgataac tcatatttaa aataaaagca
     2581 gtttattttt ttaaacataa ctaacattat tactactact atatttaatt ttaaatttat
     2641 tttttattat ataataatta gttgcgatga cacattttga gcaagttgat aaaaggcgtt
     2701 tatttgatgg tatgatagac ccaagtttag atttatttac cgaaataaac cgattgaaaa
     2761 aagaaaaaaa tgccgtatta ctagcgcatt attatcaaga tgccgatata caagacattg
     2821 ccgattatat tggcgatagt ttggg
//
LOCUS       DAOMAA010000156         2841 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_29005_length_2841_cov_2.589017, whole genome shotgun sequence.
ACCESSION   DAOMAA010000156 DAOMAA010000000
VERSION     DAOMAA010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29005_length_2841_cov_2.589017"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="PLO59_02960"
     CDS             <1..343
                     /locus_tag="PLO59_02960"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HQV00089.1"
                     /translation="LNELGIYGFIADQAPSNVKRCHWMQFLNQDTPVFFGPEKYAVQF
                     DYVVVYAHIDKVKRGHYTVTYSLITDKPQQEMPTFITEQHTKFLEKIIIVKPHLWLWT
                     HKRWKRSRPID"
     gene            complement(344..1444)
                     /locus_tag="PLO59_02965"
     CDS             complement(344..1444)
                     /locus_tag="PLO59_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003250952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HQV00090.1"
                     /translation="MIKAINIRPYIMLFALIGIWLLFSIVTDGVFLNSRNFTNLMRQT
                     AIVGILASGMVLIIVAGHIDLSIGSMVALTGGITAIANVWWGLTTPLAIVLAVLTGIV
                     LGIINGYLIAYQKIPAFIVTLGGMLIYRGIVKGLTKGETVGPLSDAMQYTGSGYLPLW
                     LSYILIAVSLASFIKYFFTLRKNNKSIATAGLITLSLSLLTVLFFTYINSYKGLPIPV
                     LVLLMVAATIAFIAMQTPFGRYVYAAGGNAEAAFYSGINIKSNLLFVYIIMGILAAVA
                     GILYTAQLGNATANAGRNLELDAIAACVIGGTSLLGGKGTIPGALLGALIMASLDNGM
                     SLLNIEDFTQDIIKGLILVTAVWLDITGGNSK"
     gene            complement(1463..>2841)
                     /gene="gguA"
                     /locus_tag="PLO59_02970"
     CDS             complement(1463..>2841)
                     /gene="gguA"
                     /locus_tag="PLO59_02970"
                     /inference="COORDINATES: protein motif:HMM:NF040905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HQV00091.1"
                     /translation="TLMKILSGVYPYGSYGGNIIWNGQEVRFKNLSQSEHAGIAIIAQ
                     ELALVPEMTIAENIFLGREICNKGIIQWDKTIAAATALMQSLSVSLNVQTKIKDCGVG
                     IQQMVEIAKALSKKAKLLILDEPTAALTETESENLLQILKKLRVQGLTAIIISHKLNE
                     VLHVAQSITVLRDGESVGTYNASSIDEEALIKSMVGRQLSDLYNKQLRKPGKQILQIS
                     NLHTWHPKTNKPIIKNVSLTVHSNQIVGIAGLMGAGRTELVETIFGAWAGKCDGQITL
                     NELKHFPKNPRQAIKNGLALVSEDRKRFGLILQQSISANTTLAALKTFSKNFLIQNKA
                     IQQATTQSVAQLKIKIHNAKQPVAHLSGGNQQKVVLAKWLLTKPSLLILDEPTRGIDI
                     GAKAEIYTIIQNLAATGVGILMVSSELPELMGLCDTIYVMNNGTINGSFETPYSAESI
                     MHYATAQH"
BASE COUNT          841 a          550 c          539 g          911 t
ORIGIN      
        1 tctaaatgaa cttggtattt atggatttat tgccgaccag gctcccagca atgtaaagag
       61 atgtcattgg atgcagtttt taaatcagga tacacctgtg ttttttgggc ctgagaaata
      121 tgccgttcag tttgattatg tagtagttta cgcacatatt gataaagtaa aaagaggaca
      181 ttatacggtt acttacagtt taattacgga taagccacag caggaaatgc caacttttat
      241 tacagagcag cacactaagt ttttagaaaa aataattatt gttaaaccac atttatggct
      301 ttggactcat aagcgttgga agcgcagccg accaatagat tagttattta gagtttccgc
      361 ctgtaatatc taaccaaaca gccgttacta gaattaaacc tttaattata tcctgggtaa
      421 aatcttcaat atttaataag ctcataccat tatccagcga ggccattatt agcgcaccta
      481 aaagcgcacc gggtatagtg cctttacctc ctaacaggct tgtgccgcct attacgcaag
      541 ctgcaatggc atctaactct aaattacggc ctgcattggc agttgcatta cctaattgtg
      601 ccgtgtataa aataccggct actgctgcaa gtatccccat tattatgtaa acaaataaca
      661 agttggattt tatattgatg cctgaataga atgcagcttc ggcattgcca ccggcagcat
      721 aaacatagcg cccaaagggt gtttgcattg caataaatgc aatggttgct gcaaccatta
      781 acaaaaccaa aaccggaatg ggcaaacctt tatatgagtt tatgtaggta aaaaataaaa
      841 cggttagcag gcttaggctt agcgtaatca aaccagccgt ggctatgctt ttattgtttt
      901 tacgaagcgt gaaaaagtat tttataaatg atgctaatga aacagcaatt aaaatataac
      961 taagccataa aggcagataa ccactaccgg tgtactgcat ggcatcgctt aaaggtccta
     1021 cggtttcgcc tttggttaag ccttttacta tacctctgta aataagcatg cctcctaatg
     1081 ttactataaa agcaggtatt ttttgatagg caataaggta gccgtttata atacccaaaa
     1141 ctatacctgt taagacggcc aaaacaattg caagtggtgt tgttaaaccc caccatacat
     1201 tggctattgc agtaataccg cctgtaagcg caaccatcga accaatgctt aaatcaatat
     1261 gaccggctac aattatgagc accatgcccg aagccaaaat gcctacaata gcggtttggc
     1321 gcataaggtt tgtaaagttg cggctattta aaaatactcc gtcagttaca atagaaaata
     1381 ataaccaaat gccgattaat gcaaaaagca tgatgtacgg tctgatatta atagctttta
     1441 tcatatagaa tcaaaataaa aatcaatgtt gtgctgtggc gtaatgcata atactttcgg
     1501 ctgaataggg tgtttcgaaa cttccgttta tggttccatt attcataaca taaattgtgt
     1561 cgcataagcc cattagctcg ggtagttcgg aacttaccat aagtatgcct acacctgtgg
     1621 ctgctaaatt ttgaatgatg gtgtaaattt cggctttggc accaatatca atacctctgg
     1681 taggttcgtc taaaatcaat aaagacggtt tggttaataa ccattttgct aaaactactt
     1741 tttgctgatt gcctccactt aagtgcgcaa ccggttgctt cgcattatga attttaattt
     1801 ttaattgtgc aacactttgt gtggttgcct gttgaatggc tttgttttga attaaaaagt
     1861 ttttagaaaa agtttttaat gcagctaaag ttgtattggc tgaaatgctt tgttgcaata
     1921 tgagtccgaa acgttttctg tcctcgctta ccaatgccaa tccatttttt attgcctgac
     1981 gcggattttt aggaaagtgt tttaattcat tcagcgttat ttggccatca catttacccg
     2041 cccatgcacc aaaaattgtt tcaactaact cggttcgacc tgcacccatt aaaccggcaa
     2101 tgcccacaat ttgattactg tgtacggtta acgatacatt tttaataatc ggtttattgg
     2161 ttttgggatg ccaggtatgg aggttgctta tctgtaaaat ttgcttgccg ggcttacgta
     2221 attgtttatt atacaaatcg gatagttgac gcccaaccat gctttttata agtgcctctt
     2281 cgtcaatact acttgcgttg tatgtaccaa ccgattcgcc atcgcgcagt acggtaatgc
     2341 tttgggcaac gtgaagtact tcatttagtt tgtgtgatat aataatagcc gtaaggcctt
     2401 gcacacgtaa tttttttaaa atctgtaaca ggttttctga ttcggtttcg gtaagggctg
     2461 cagtgggttc atctaaaata agcaacttag ctttttttga taaggcttta gcaatttcaa
     2521 ccatttgttg tatgcccact ccgcaatcct ttatcttggt ttgtacattt aagcttacac
     2581 ttaacgattg cattaatgca gttgccgctg caattgtttt atcccattgt atgattcctt
     2641 tgttgcaaat ttcgcggcct aaaaaaatat tttcggctat ggtcatttca ggtactaaag
     2701 caagttcctg tgctatgatg gcaatgccag catgctctga ttgactaagg tttttaaatc
     2761 gaacttcttg tccgttccaa ataatattgc cgccataaga accgtacgga tatacacccg
     2821 ataaaatttt catcagtgtt g
//
LOCUS       DAOMAA010000157         2837 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_29075_length_2837_cov_2.879942, whole genome shotgun sequence.
ACCESSION   DAOMAA010000157 DAOMAA010000000
VERSION     DAOMAA010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29075_length_2837_cov_2.879942"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..882
                     /locus_tag="PLO59_02975"
     CDS             139..882
                     /locus_tag="PLO59_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HQV00092.1"
                     /translation="MQIYDIIIKNCRQKKKQIAVLVDPDKIDEKGVINLATLAQKFCP
                     DFIFVGGSLLTNGSLSNVVSLLKMHCTIPVILFPGNILQIEAQADAMLLLSLVSGRNA
                     ELLIGNHVVAAPILKEKKLQTISCGYMLIESGNITTALYMSNTQPIPNTKKDIAACTA
                     LAATMLGMKTIYMDAGSGAALPVPASMIEAVSAEVDVPLIVGGGIKTVQQAQAAWHSG
                     ADIVVVGNAIETDILMMEQMIEAAKVFNA"
     gene            879..1433
                     /gene="pnuC"
                     /locus_tag="PLO59_02980"
     CDS             879..1433
                     /gene="pnuC"
                     /locus_tag="PLO59_02980"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HQV00093.1"
                     /translation="MISSLEFIGACFGVLGIWLTAKKNVLCFPVGIINVLITANVVFE
                     VKLYADVVQQLMYLVLLVYGWINWTKSQNIKSVSYCTVTQKIFVVVITLIIGTVGYFI
                     LKKYTNATYPAADAYATAVCFAAQFLIAKRKIENWWLWLFANLVYVVIFYLKQMPWYA
                     GLSVIYFLQAIFGLKQWHKQLKTI"
     gene            1406..1990
                     /locus_tag="PLO59_02985"
     CDS             1406..1990
                     /locus_tag="PLO59_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQV00094.1"
                     /translation="MAQATQNHIVNHIAIVGPESTGKSVMAKQLAQIYNGIYVEEYAR
                     MYLEQLDRNYNEQDLLLIAKQQLLLEEEALSIYKNNSNKLQPFIFYDTTLLVIKIWSE
                     FKYGQCNAWILQMLKKRKYNLQLLMDIDLPWEFDPIREHPDKRQILLERYQAELKKPH
                     ANYHTVNGLEKERLQNAVAIINQQIELGVLINNG"
     gene            2048..>2837
                     /locus_tag="PLO59_02990"
     CDS             2048..>2837
                     /locus_tag="PLO59_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family peptidase"
                     /protein_id="HQV00095.1"
                     /translation="MKIHFTFCLLLVLSQLNAQHGANHQGKFEQLGQTLADPNMYRSA
                     SGAPGPKYWQQRADYNINATLTESDQRIRGSETITYYNNSPEILTFLWLQLDENVFVN
                     TNEANTQNTSKMNENMSLKDVRNLANAPNDLGIKIEAVTDVGGKALHYVINETMMRVD
                     LPKPLKTGEKFAFKVTWNYRMVNRIERWGRGGYEYFSDDSNYMFTIAQWYPRMCVYSD
                     WQGWNHKQFTGTAEFALTFGNFDVSLTVPADYMVAGTGQCSNYAS"
BASE COUNT          939 a          467 c          583 g          848 t
ORIGIN      
        1 aaattttgtg cgaaagtata taattaaaat aggctttcaa ataaatgacc tatcaagtga
       61 agtaaccatg ttggtttgat ttatcttttt tgccaatatg ttttttgaat ttgaagttat
      121 acttaccaac ttgcagccat gcagatttat gatataatta taaaaaattg ccggcaaaag
      181 aaaaagcaga ttgcagtact tgttgatccc gataaaattg atgaaaaagg agtaattaac
      241 ttagccactt tggcccaaaa attttgtccc gactttattt ttgtgggtgg tagtttgctt
      301 acaaatggaa gtttatccaa tgttgtttcg ctgctaaaaa tgcattgtac gataccggta
      361 attttatttc cgggcaacat actacaaatt gaggcgcagg cagatgccat gttactgcta
      421 tcattagtga gcgggcgaaa tgctgaatta cttataggta atcacgtagt tgccgcaccg
      481 attttaaaag aaaaaaagct tcaaacaatc agttgtggct atatgcttat tgaaagtgga
      541 aatattacaa cagccttata tatgagcaat acgcaaccca tacccaatac aaaaaaggac
      601 attgcagctt gtacagccct ggctgctact atgttaggta tgaagaccat ttatatggat
      661 gcaggcagtg gtgctgccct gccggtaccc gcatcaatga ttgaagccgt aagtgccgaa
      721 gttgatgtgc cacttatagt tgggggtggc ataaaaacgg tgcaacaggc tcaggcagcg
      781 tggcatagtg gtgctgatat tgttgtggta ggtaacgcta ttgaaacgga tattttaatg
      841 atggaacaaa tgattgaagc tgccaaggtt tttaatgcat gatttcttcg ttagagttta
      901 tcggggcttg ttttggcgtt ttaggtattt ggcttactgc aaaaaaaaat gtgctgtgtt
      961 ttccggtagg tataatcaat gtgttaatta cagctaatgt tgtatttgaa gtaaagctat
     1021 atgccgatgt tgttcagcag ctaatgtatt tagtgttatt agtatatggt tggataaatt
     1081 ggacaaaatc tcaaaacata aagagcgtaa gctattgtac agttactcaa aaaatatttg
     1141 ttgtggtaat cactttgatt attggcactg ttggttattt tattttaaaa aaatatacaa
     1201 atgcaaccta tccggcagcc gatgcctatg caactgctgt ttgttttgct gcacaatttt
     1261 taatagccaa acgcaaaatc gaaaactggt ggctgtggtt atttgcaaat ttagtttacg
     1321 tggtaatttt ttatttgaaa caaatgccct ggtatgctgg cctatctgta atatattttt
     1381 tgcaagcaat ttttggtctt aaacaatggc acaagcaact caaaaccata tagtaaatca
     1441 tattgccatt gtgggtcctg aaagtacagg taaatcggtt atggcaaagc aacttgcaca
     1501 aatttataac ggcatttatg ttgaagaata tgcacgtatg tatttggaac aattagacag
     1561 aaattataat gaacaagatc ttttattaat agcaaaacaa caattattgc tagaagaaga
     1621 ggcattaagc atatacaaga ataatagtaa caaactgcag ccttttattt tttacgatac
     1681 aacattactt gtaataaaaa tctggagtga atttaaatat gggcaatgca atgcttggat
     1741 tttgcaaatg cttaaaaaaa gaaagtacaa cctgcaatta ctaatggata tagatttgcc
     1801 ttgggagttt gatccaatac gcgaacatcc cgacaagcgg caaatactgc tggaacgcta
     1861 ccaagcggaa ctgaaaaaac cacatgctaa ttatcatacc gtaaatggac ttgaaaagga
     1921 aaggctgcaa aatgcggtag caataattaa tcaacagata gagttagggg tattgataaa
     1981 taacggttaa aaaaaaatga aggaattata aaacaatact tttagcacca tccaaaattt
     2041 gcgcaatatg aaaatacact ttaccttttg tttactcttg gtacttagtc agctaaatgc
     2101 acaacatggt gctaatcatc aaggcaaatt tgaacaatta gggcaaacgc tggccgatcc
     2161 gaatatgtac cgttcagcat cgggtgcgcc tgggcctaag tactggcaac aaagagccga
     2221 ttataatatt aatgcaacac ttaccgagtc ggatcagcgc attaggggta gcgaaacaat
     2281 tacttattat aataactcgc ccgagatact tactttttta tggcttcaac ttgacgaaaa
     2341 tgtttttgta aataccaatg aagctaatac gcaaaacaca tcgaaaatga atgaaaacat
     2401 gagtttgaaa gatgtgcgca atttagctaa cgcgcccaac gatttgggga taaaaattga
     2461 agccgttaca gatgttggtg gcaaagcttt gcactacgta attaacgaaa ccatgatgcg
     2521 tgttgatttg ccaaaacctt taaaaacagg agaaaaattt gcattcaaag taacatggaa
     2581 ttaccgcatg gttaatcgca tcgaacgctg gggtcgtgga ggctatgagt attttagcga
     2641 tgacagtaat tacatgttta ccattgcaca atggtatcca cgtatgtgtg tttacagcga
     2701 ttggcagggt tggaaccaca aacaatttac aggcacagcc gaatttgcac ttacgttcgg
     2761 aaattttgat gtaagcctaa ccgtacctgc tgattatatg gttgcaggta ccggccaatg
     2821 cagtaactat gcttcgg
//
LOCUS       DAOMAA010000158         2833 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_29153_length_2833_cov_3.182505, whole genome shotgun sequence.
ACCESSION   DAOMAA010000158 DAOMAA010000000
VERSION     DAOMAA010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29153_length_2833_cov_3.182505"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="PLO59_02995"
     CDS             <1..224
                     /locus_tag="PLO59_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00096.1"
                     /translation="IVVSMEYPGNPTPVYYEFFDATTAMKDANTWTKLNATFYLPKTI
                     DPNISFKAFVWNKGQTIAYADDFALRFVE"
     gene            237..1601
                     /locus_tag="PLO59_03000"
     CDS             237..1601
                     /locus_tag="PLO59_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00097.1"
                     /translation="MRNNSYSILVIVFIALAIIRADVKDAFWKINFVITADMIGYYEY
                     LPATIIHQDATLQFLKDTTIANYKRYQHVPMANGNLLNKFSCGVAIMQAPFFALAHGM
                     APILNQQQDGWSDIYEIMVLVAGAFYFVMGLVLLRKLLLNYFNDFYVAIALLTVYFGT
                     NAILYTTAQAGMSHAYSFFLFAAFMLLTCKWYDKPTLLKTAGLGIVAGLIFLIRPTNA
                     ISFLFFIGYDVYTKDLLKARIKFLTQNLKSIFLLALVAVAVMIPQWIIWKNISGSYFF
                     YSYIGESFFFLQPQFFSSLFSYRNGWLIYSPLAALGLAGLFLLKKYARPVATVFPIII
                     VLMLYVITSWWCWWYVGFGNRAFVEMQALLAFGYAALFTWLSQKNRWLSYLLYVVCFF
                     GFYLNSFQCHQYKYGLIHWDAMTADAYKAIFLQDSKPPGYDYLLSAPDYQQTLVKREP
                     FFYQ"
     gene            complement(1622..>2833)
                     /locus_tag="PLO59_03005"
     CDS             complement(1622..>2833)
                     /locus_tag="PLO59_03005"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HQV00098.1"
                     /translation="HRKILLVFSVCINLGLLIYFKYSNFFIENVNVLLAGIGFNQIHW
                     TKLILPIGISFYTFETMTYVVDVYRRVHKPLKNFWEYQLYIILFPKLIAGPIIRFHEL
                     ADQINDRRSTTTIDHVLTGFYRFVLGLAKKVIIANTMGMYADQIYGLDAATVSTLTAW
                     LAALAYTMQIYFDFSGYSDMAIGLGKMMGFTFPENFNNPYTSQNITEFWRRWHITLGA
                     WMRNYLYIPLGGNKVSSTGRLYFNLWLVFLASGLWHGASWSFIFWGCYHGLFLVLERA
                     GLSKFYNNIGRIPSMIITFVIVLTGWVVFRIEDFGVAAVFLKKMYAFNFNAPLQFPVF
                     DFWSTLLVAIIFSFFVLPVKGQKLQHRIFFGNYTVKRHLFMTCVSAILLFISATAILG
                     SSFNPFIYFRF"
BASE COUNT          904 a          534 c          494 g          901 t
ORIGIN      
        1 ttattgtagt tagtatggaa tacccgggca accctacacc tgtttattat gagttttttg
       61 atgcaaccac tgctatgaaa gatgcaaaca cctggacaaa attaaatgcc actttttacc
      121 tgcctaaaac cattgaccct aatatttcgt ttaaagcatt tgtttggaat aaaggccaaa
      181 ccattgctta tgccgatgac tttgctttac gttttgttga gtaacactta ctatacatgc
      241 gcaataatag ttattccata ttagttatcg tttttatagc attggcaatt ataagagcgg
      301 atgttaaaga cgctttttgg aaaataaatt ttgtaataac agctgatatg attggctatt
      361 acgaatattt gcctgctacc ataattcatc aggatgcaac actacagttt ttaaaagata
      421 ccaccattgc taattacaaa cgctatcagc atgtgccaat ggctaatggc aacttattaa
      481 ataaatttag ttgtggggta gccattatgc aagcaccgtt ttttgcattg gcacatggca
      541 tggcaccaat tttaaatcaa cagcaagacg gttggagtga tatttatgaa ataatggtat
      601 tggttgcagg tgcattttac tttgtaatgg gattggtgct gctacgcaaa ttgcttttaa
      661 actatttcaa cgacttctat gtagctattg cattactcac tgtatatttt ggcaccaatg
      721 ccattttata cactactgca caagctggca tgtcgcatgc atattcgttt tttttgtttg
      781 ctgcttttat gttgcttaca tgtaagtggt atgataaacc aacactacta aaaactgcag
      841 gcttgggaat tgtggccggc ttgatttttc tgatacggcc taccaatgca atttcgtttt
      901 tattctttat cggatatgac gtttacacaa aagatttatt aaaagcacgc ataaaatttc
      961 tgacacaaaa tttaaagtct atttttttat tggctttggt tgccgtagca gtaatgatac
     1021 cgcaatggat tatttggaaa aacatttcgg gcagttactt tttctacagc tacattggcg
     1081 agtcgttttt ctttttacaa cctcaatttt tttcgtcatt gttctcatac cgtaatggct
     1141 ggcttattta ttcgccatta gctgcattgg gcttggcagg cttgttttta cttaaaaaat
     1201 atgcaaggcc tgtagctact gtttttccga ttataattgt attgatgctg tatgtaatta
     1261 ccagttggtg gtgttggtgg tatgtaggtt ttggcaatcg tgcatttgtt gaaatgcagg
     1321 cacttttagc ttttggttat gcggctttgt ttacctggct cagccaaaaa aacagatggc
     1381 tatcgtacct gctatacgtt gtttgttttt ttgggtttta tctcaatagc tttcaatgcc
     1441 atcaatacaa atacggactc atacattggg atgctatgac tgccgatgca tacaaagcga
     1501 tatttttaca ggatagcaaa ccaccgggat atgactactt gcttagcgcg cctgattatc
     1561 aacaaacctt agttaagcgc gagccttttt tctatcaata attgcttaca acaacttagg
     1621 tttaaaacct gaaatagata aaaggattaa agctcgaacc taagatggct gttgcagaaa
     1681 taaacaaaag tattgccgat acgcatgtca taaacaaatg acgtttaacg gtgtagttac
     1741 caaaaaatat acggtgctgc aatttttgtc cctttaccgg caaaacaaaa aatgaaaaaa
     1801 taattgccac taataaagtg gaccaaaagt cgaaaaccgg aaattgtaat ggggcattaa
     1861 aattaaatgc atacatcttt tttaaaaata ctgctgccac gccaaaatct tcgattcgaa
     1921 aaaccaccca acccgtaagt acaattacaa aagttataat catagatggg atgcgaccaa
     1981 tgttgttgta aaattttgac aaccctgcac gttctaaaac taaaaacaaa ccatgatagc
     2041 atccccaaaa aataaaactc cacgatgcgc catgccataa ccctgaggct aaaaaaacca
     2101 gccataaatt aaaatacaaa cggcctgtgc tacttacttt attgcctcct aatggtatgt
     2161 aaaggtaatt acgcatccag gcacccaggg ttatatgcca cctgcgccaa aattcagtta
     2221 tattttgcga tgtatatgga ttattaaaat tttcgggaaa tgtaaaaccc atcatcttac
     2281 ccaaaccaat tgccatatcg ctatagcccg aaaagtcgaa ataaatttgc atggtatatg
     2341 ccaatgcagc caaccatgcc gttaatgtac ttaccgtagc tgcatctaaa ccataaatct
     2401 gatcggcata catgcccatt gtatttgcaa taattacctt ttttgccaag cctaaaacaa
     2461 aacgatagaa gccggttaaa acatggtcaa ttgtagtagt acttcgcctg tcatttattt
     2521 gatcggcaag ttcgtgaaac ctgattatag gcccggcaat taatttggga aaaagaataa
     2581 tgtataactg atactcccaa aaatttttca atggcttatg tacacgtctg taaacatcaa
     2641 ccacataagt catggtttcg aaagtgtaaa acgatatacc aatgggcagt atcagtttag
     2701 tccaatgtat ttgattaaag ccaataccgg ccaacaatac atttacattt tcgataaaaa
     2761 aatttgaata cttaaaataa atcaacaagc ctaaattaat gcaaaccgaa aacaccaata
     2821 atattttacg atg
//
LOCUS       DAOMAA010000159         2828 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_29240_length_2828_cov_2.742878, whole genome shotgun sequence.
ACCESSION   DAOMAA010000159 DAOMAA010000000
VERSION     DAOMAA010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29240_length_2828_cov_2.742878"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1229)
                     /locus_tag="PLO59_03010"
     CDS             complement(<1..1229)
                     /locus_tag="PLO59_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional class I SAM-dependent
                     methyltransferase/glycosyltransferase family 2 protein"
                     /protein_id="HQV00099.1"
                     /translation="MRYLAYFDALAAKRIALRKRNAYYWNDITRYCNYFSHDDYNVLE
                     IGCGTGELIHEIKGRNKTGIDFSAAMIEQAKAQFDDVSFYQMPAEQITLNQTYDLVIV
                     SNLIGFVDDVQKVFEQLRLVCHANTKIIITYYNYFWEPFIKFAEVIGLKTNTPNQNWL
                     SQADIKNLLSLAGFDTYRRVNSMLLPFNVPLISWTLNRFLVRLPFFKLFALNMYSFAT
                     LKPEAKANNEQYSVSIVIPARNEAGNIENAITRLPKFGKHQEIIFIEGNSTDNTWQVI
                     QTIAQKYAATHTIKIGQQTGRGKNDAVKKGFAMATGDILMILDADLTVPPEDLPKFYY
                     AIANGHGDFINGSRLVYQMDKEAMRFLNLLGNKFFSLAFSWLLEQPFKDTLCGTKVIF
                     RHDYYRLVSNQAYFGNF"
     gene            1422..1877
                     /locus_tag="PLO59_03015"
     CDS             1422..1877
                     /locus_tag="PLO59_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00100.1"
                     /translation="MKPYVAGHVFKVSLPNYMKRTIGYNDDAAIQFKNSVKDVYGFII
                     EDNKAEMELAEIVYASINEFYIDFSKDFLADEKKRTSTLPTENKTGDVKFIYNEVSYY
                     DKEAKSMITYFVGIAETKDAFYKMLCWTTTDNKDLYRNDFEKILFSIND"
     gene            1938..2432
                     /locus_tag="PLO59_03020"
     CDS             1938..2432
                     /locus_tag="PLO59_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQV00101.1"
                     /translation="MIKRMLIFVLFLQTAHAQNAETNIHALLNQWHAAAAQAQFENYF
                     KPLHKDFIFMGTDATERWDMAAFKAFAKPYFDKGKAWSFTTLERKVYVTSDGNYAWFD
                     ELLQTQMKICRGSGVLLKTNNQWQLVQYVLSVTVPNDCIDAAIKIKAPIEDALIESLR
                     NKKQ"
     gene            complement(2442..>2828)
                     /locus_tag="PLO59_03025"
     CDS             complement(2442..>2828)
                     /locus_tag="PLO59_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQV00102.1"
                     /translation="DVVFGSSVLHHLEFEASLNEIYRVLKPGGRMVFAEPNMLNPQIF
                     IQKNVPFIKTWLGDSPDETAIVRWQFANLLKTHGFKNVEIFPYDFLHPIVPKPLINVV
                     NGIGKFIEKVPVLKEIAGSVIIYAEK"
BASE COUNT          932 a          510 c          486 g          900 t
ORIGIN      
        1 tcgaagttgc cgaaataagc ctggttactc actaagcgat aataatcgtg tctgaaaata
       61 actttagtgc cacagagtgt gtctttaaaa ggttgttcga gcagccatga aaaggcaaga
      121 ctaaaaaatt tattacccaa caagtttaaa aaacgcattg cctccttatc catttgatac
      181 accagtcgag atccattaat aaagtcgcca tggccattag caatagcata ataaaattta
      241 ggcaagtctt cgggcggcac tgttaaatcg gcatctaata tcatcaatat gtcaccagta
      301 gccattgcaa accctttttt taccgcatca tttttacccc tacccgtttg ttgtccgatt
      361 tttatcgtat gtgttgctgc atatttttga gcaatggttt gtattacctg ccaggtatta
      421 tcggttgagt tgccttctat aaaaattatt tcctgatgct taccaaactt gggtaaacgc
      481 gtaattgcat tttcaatatt tccggcttcg ttgcgtgcag gtattactat gcttacactg
      541 tattgctcat tgtttgcttt tgcttcgggt tttaaagttg caaacgaata catgttaagt
      601 gcaaacaatt taaaaaaagg caagcgcact aaaaatcggt tcaatgtcca tgatattaat
      661 ggtacattaa atggcaacaa catgctgttt accctgcgat aggtatcgaa accggctaat
      721 gaaagtaagt ttttaatatc agcctgactt aaccagtttt gattaggtgt attggttttt
      781 aggccaataa cttcagcaaa ttttataaaa ggctcccaaa aataattgta ataagttatg
      841 ataattttag tgttggcatg gcataccaaa cgcagttgtt caaacacctt ttgtacatca
      901 tctacaaaac caattaggtt tgatacaatt accaaatcat aggtttgatt taatgtaatt
      961 tgttcggcag gcatttgata aaatgataca tcatcaaact gtgcttttgc ttgttcaatc
     1021 attgcagcac taaagtcgat gcctgtttta tttcggcctt ttatttcatg tatcagttcg
     1081 cctgtaccgc agccaatttc caacacatta taatcatcat gcgaaaagta attgcaatag
     1141 cgggtaatgt cgttccagta atatgcattt cgttttcgca aagcaatgcg ctttgcagcc
     1201 aatgcatcaa aataagctaa gtatcgcaag tgaataaaat ttataattgc tgcgaattaa
     1261 aaataaaatg catgcaatta ctaatcacac ctaactacca agcattgttg ccaatacata
     1321 atattttgtt ctaagtttac acgcaaactt taattgttat gaaattaatt acgttagtca
     1381 tgctatgctt tataacaaat tgcttacatg cacaaacaga aatgaaacct tatgttgccg
     1441 ggcatgtgtt taaagtgagc ttgcccaatt acatgaaacg caccatagga tacaatgacg
     1501 atgccgctat tcaatttaaa aacagtgtta aagatgttta cggttttata attgaagaca
     1561 ataaagccga aatggaattg gccgaaattg tttatgcatc tattaatgaa ttttatattg
     1621 atttcagcaa agactttttg gcggatgaaa aaaaacgaac ttcaacgctg ccaactgaaa
     1681 acaaaactgg tgatgttaaa tttatttaca acgaagtttc gtattatgat aaagaggcca
     1741 aaagtatgat tacctatttt gttggtattg ccgaaacaaa agatgccttt tataaaatgc
     1801 tttgctggac cactacggat aataaagatt tgtaccgtaa cgatttcgaa aaaattttat
     1861 ttagcataaa tgattaatct gcttcaaaat tgcacttatg ctaattgttt aacttactga
     1921 ttaaatcgta tagccctatg attaaaagaa tgttgatttt tgtattgttt ttgcaaacag
     1981 cacatgctca aaatgctgaa accaatatcc atgctttatt aaaccaatgg catgctgcag
     2041 ccgcacaagc tcaatttgaa aattacttta agcccttgca taaggatttt atttttatgg
     2101 gcaccgatgc tactgaacgt tgggatatgg ccgcatttaa agcatttgcc aaaccctatt
     2161 ttgataaagg caaagcctgg agttttacca cacttgagcg aaaagtttat gtaaccagcg
     2221 atggtaatta cgcctggttt gatgaattgt tgcaaacaca aatgaaaatt tgccgcggtt
     2281 cgggtgtctt attaaaaaca aacaatcaat ggcaactggt tcagtatgta ttatcggtta
     2341 cagtacctaa tgattgtatt gatgcagcaa taaaaataaa agcccctatt gaagatgcgt
     2401 taatagaaag cttgcgaaac aaaaagcaat aaaacgcaat tctatttttc ggcatagata
     2461 attaccgagc cggcaatttc ctttaatacg ggcacctttt caataaactt gcctatgcca
     2521 ttaactacat taattaaagg ctttggcaca atgggatgca aaaaatcgta aggaaaaatt
     2581 tctacatttt taaaaccatg cgtcttcaat aaattagcaa actgccagcg tacaattgct
     2641 gtttcatcag gcgaatcgcc taaccatgtt tttataaaag gtacattttt ttgaataaaa
     2701 atttggggat tcagcatatt aggttcggca aaaaccatgc ggccacccgg ttttaaaact
     2761 ctgtaaattt cgtttaatga tgcttcaaat tctaaatggt gcaacaccga actaccaaaa
     2821 acaacgtc
//
LOCUS       DAOMAA010000160         2823 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_29330_length_2823_cov_3.548410, whole genome shotgun sequence.
ACCESSION   DAOMAA010000160 DAOMAA010000000
VERSION     DAOMAA010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29330_length_2823_cov_3.548410"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(184..1986)
                     /gene="mutL"
                     /locus_tag="PLO59_03030"
     CDS             complement(184..1986)
                     /gene="mutL"
                     /locus_tag="PLO59_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017256747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HQV00103.1"
                     /translation="MDIIKLLPDAVANQIAAGEVIQRPASAVKELLENAIDAGADTIK
                     LIIKDAGKTLIQVIDNGSGMSDTDARMSFERHATSKLQNADDLFNIRTKGFRGEALAS
                     IAAIAQVTLSTRRYDDEIGTEIVIEGSDVKSQQAVATPAGTTFSVRQLFFNTPARRNF
                     LKSNAQEMRHVLEEFMRVAIPHFNIAFSLFHDNTEVFQVKAANLRQRICNLLGANYNE
                     RLVPVEEETSIVKIFGFIGKPEHAKKVRGEQYFFINNRFVRDGFLNHAVNNGYEGLIP
                     KDAHPSYFIFIEVNPASIDVNIHPTKTQIKFEVERSVYAIIRSAVKRALGKFSVAPSL
                     DFEHSTTFDLPLSQLQQQPKQPQISINPDYNPFKTTTDFNPRHNVESSTHKINFEQWQ
                     ALQQQIETGAQQINSSSQNALYTNQNCFQVLNKYIVFVHTMGLVMVNQQAAHERVLFE
                     NNKAAFQNKTNSSQQSLFPQTMQLTAENFVIMQELESDLKQLGFDLNVFGKDTYLVHG
                     IPTWATKGSEVTLLESILEQFKMNSKSFRLELRNNLAKSMARAASIKVGTALSDAEMK
                     SLLTDLFQSNLPNDGIYGGKIFTLLQADELDKLV"
     gene            complement(2177..2692)
                     /locus_tag="PLO59_03035"
     CDS             complement(2177..2692)
                     /locus_tag="PLO59_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00104.1"
                     /translation="MKKIVASVSCFALAITLSQAQQLVVLKNGDRIAGKIVSVTNDVV
                     NFATKTDGTKGYKTNEISLIYFDENLVPKAQLSNSKLPSGTVTADVPGRKITKTPDMK
                     ILTQDKGIVVVNIIVDKYGRVKKAEPGGEGTNTTSGYLLTKAKQAAEATIFDTDPTYP
                     LETKGTITVVF"
BASE COUNT          837 a          541 c          479 g          966 t
ORIGIN      
        1 ttgaaattgt ttaaagaata tactataaat tttctaattc taataattta tatgaaaaat
       61 aaaatttatt acaaaaaaat tagattccaa ggttccttgc aatgttttca tcacaacatt
      121 tccaccaatt tttaaattat ttaaacttat aatttaagat gccatgaaac cggttaaaac
      181 aactcaaacc aatttgtcca attcatctgc ttgcaataag gtaaaaattt tccctccgta
      241 aatgccatca tttggcaaat tactttgaaa caaatctgtt agcaatgatt tcatttcggc
      301 atccgatagt gctgttccta ccttaattga agctgcacgt gccatactct tagctaaatt
      361 gtttcgtaat tcaagtctga aacttttact gttcatttta aattgctcta aaatactttc
      421 cagcagggta acctcactcc cctttgtagc ccatgttggt atgccatgta ccaaataagt
      481 gtctttacca aaaacgttta aatcaaaacc caattgtttt aagtcggatt ccaactcttg
      541 catgattaca aaattttcgg cagttaactg catggtttgc ggaaacaaac tttgttggct
      601 gctgttagtt ttattttgaa atgcagcttt attgttttca aataaaacac gctcatgtgc
      661 ggcttgttgg ttaaccatta ccaaacccat cgtgtgtaca aatacaatgt atttgtttaa
      721 tacctgaaag cagttttgat tggtgtaaag tgcattttga ctggatgaat ttatttgttg
      781 cgcacctgtt tcaatttgct gttgtaaagc ttgccattgc tcaaaattaa ttttatgagt
      841 actgctttct acattatgcc tggggttaaa atccgtagtt gttttaaacg gattgtagtc
      901 aggattaatg gatatttgtg gctgcttggg ctgctgttgc aattggctta gtggcaagtc
      961 gaaagtagta ctgtgttcaa aatctaacga gggtgctaca ctaaatttac ccaaggcacg
     1021 ttttacagca cttctgatga tagcataaac cgacctttca acttcaaact ttatttgtgt
     1081 tttggtagga tgtatgttta catcaatact tgcaggattt acttcaataa aaataaaata
     1141 cgatgggtgt gcatctttag gtattaatcc ttcatagcca ttatttacgg catggtttaa
     1201 aaaaccatcg cgtacaaaac ggttattaat aaaaaagtac tgctcgcctc gtactttttt
     1261 ggcatgctcg ggtttgccaa taaatccaaa aatttttaca atggaggttt cttcttcaac
     1321 cggtaccaat cgctcattat aatttgcgcc taacaaatta caaatgcgct gccgtaggtt
     1381 tgcggcttta acttgaaaca cttcggtatt gtcgtgaaac aaactaaaag caatattgaa
     1441 atgcggtatt gctacacgca taaactcctc cagtacatgc cgcatttctt gtgcattgct
     1501 ttttaaaaaa tttctgcggg ccggtgtatt aaaaaacaat tgtcttacgc taaaggtagt
     1561 accagccgga gtggctacag cttgttgtga ttttacgtca gaaccttcga ttacaatttc
     1621 agtgcctatc tcatcatcat acctgcgtgt gcttaatgtt acttgtgcaa ttgcagcaat
     1681 actggccaat gcttcgcccc taaacccctt ggttctgata ttaaacaaat catcggcatt
     1741 ttgtaattta ctggtagcat gccgttcaaa actcatgcgc gcatccgtat cgctcatacc
     1801 actgccatta tcaattacct gtattagtgt tttaccggca tctttaataa ttaatttaat
     1861 agtatctgcc ccagcatcaa tagcattttc aagcaattcc tttacggctg aggccggtcg
     1921 ttgtattact tcgcctgctg caatctgatt tgctacggca tcgggcaaaa gcttaatgat
     1981 atccataaaa tgaaagcctg tttaaattca aacttaaaaa aaaagtggct gatataaata
     2041 cagccacttc aggtataaag atctgtgtgt gttaaacgtt gcaaatatat gttaaaactt
     2101 atgtaaatgg tattatcctt cgtaatccta tcaaataaag tataatcgcg ttttacttaa
     2161 ctattccaac atcaaattaa aacacaacgg taatggtacc ttttgtctcg agcggatagg
     2221 tagggtcggt atcaaaaata gtagcttcgg cagcttgctt tgccttggtt aaaaggtaac
     2281 cggatgtggt gttggtgcct tcgccaccgg gttcggcttt tttaacccga ccatatttat
     2341 ctacaataat atttaccaca acaatgcctt tgtcctgagt tagtattttc atatcggggg
     2401 tcttggtaat tttgcgtccg ggcacatcgg cagttaccgt acccgatggt agcttgctat
     2461 tactcaactg ggctttaggt actaggtttt cgtcaaaata aataaggcta atctcattgg
     2521 ttttataacc cttagtacca tcggttttag tagcaaagtt taccacatca ttggtaaccg
     2581 aaacaatttt acccgctatc ctatcgccat tttttaatac tactaattgt tgtgcttgcg
     2641 ataaggttat agctaatgcg aaacaactaa ctgaggctac aatttttttc attcttaaaa
     2701 ttttttgata atacaatttt gtttaattac aagattaata acgtaacaaa cgtagtgcaa
     2761 ttggcaacac tattattttt accaacaaaa actttattaa cacattcatt ttaatttagc
     2821 gcg
//
LOCUS       DAOMAA010000161         2821 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_29381_length_2821_cov_2.741142, whole genome shotgun sequence.
ACCESSION   DAOMAA010000161 DAOMAA010000000
VERSION     DAOMAA010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29381_length_2821_cov_2.741142"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..117
                     /locus_tag="PLO59_03040"
     CDS             <1..117
                     /locus_tag="PLO59_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009281094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HQV00105.1"
                     /translation="NDQNKTVVFPKQHYKKGDYVNVMVTDCTAATLLGEAIV"
     gene            151..1350
                     /locus_tag="PLO59_03045"
     CDS             151..1350
                     /locus_tag="PLO59_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HQV00106.1"
                     /translation="MELQAIKARFGIIGNSPLLEHAIDTARQVAPTEITVLITGQSGV
                     GKEVFPQIIHQLSSRKHGPYIAVNCGAIPEGTIDSELFGHEKGSFTGAHEARKGYFET
                     ANSGTIFLDEVAELPLGTQVRLLRVLETGEFLKVGSSKVQKTNVRVVAATNINMPQAI
                     ANGKFREDLFYRLNTVPISIPSLQQRKDDIPLLFRKFASDFAEKYRMSTKQLDADAMQ
                     MLIAYPFPGNIRQLRNLCEQICILEKNPVVTTQIPTHYLPQVNTNTLPVLLRDENKNS
                     HADFANERDLLYKVLFEMKKDINDLKRVVYDNMPVSDNFENIPSPHNAIIDMQGIMPT
                     GSVTLQTHPQQMAEEVEESLSISEKEKELIVKALQKHRNRRNNAAKELGISERTLYRK
                     INEYGLK"
     gene            1367..1876
                     /locus_tag="PLO59_03050"
     CDS             1367..1876
                     /locus_tag="PLO59_03050"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HQV00107.1"
                     /translation="MKKLINGWNFSVLGLLVFLTSGCYSFKSGRIPDNIETINIKFFP
                     NRAAVVQPSLSQIFTDKLKDKFVSESRLNLITTHADLNFEGYISDYTNTPNAIQNSQQ
                     AALNRLTISVFVKFTNAKDPKQDFETTFTRFAEYNSSLSFQTVETNLIQEINRLLIDD
                     IYNKSVTNW"
     gene            2067..>2821
                     /locus_tag="PLO59_03055"
     CDS             2067..>2821
                     /locus_tag="PLO59_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00108.1"
                     /translation="MHRNQFLSYIENPKHAPWHTDVFSPLLAQFPYCQSLHLMYAKHL
                     HYIEHFNYQQQLKLTAAYAGNRSVLHALINDPTYQSVTTSSVMAVEQENNAASNVFTE
                     APKHHSNVFVLDELETTTATTNNNTAQSIAAANATTQWSDTNNLQPTDLPANDNLFNL
                     EETVEEIQATVLEETTKDELDSLTHLKLHNLNKNATTSDNDSAVEVDLKNEIADEIIV
                     PSDAVKVEYSNQNKTSAITLEAETNAESTEVLTV"
BASE COUNT          994 a          574 c          496 g          757 t
ORIGIN      
        1 aacgatcaaa ataaaaccgt tgttttcccg aaacaacatt ataaaaaggg cgactatgta
       61 aatgtaatgg taaccgattg tacagcagct acattattgg gcgaagccat tgtttaatta
      121 aattgaagcg caaaacttgc aatcaaaaaa atggaattac aagcaattaa agcacgattt
      181 ggtataattg gtaactcacc attattagag catgccatag acacagcccg gcaagtggca
      241 cctactgaaa ttacggtttt aattacaggg caaagtggtg tgggtaaaga agtgtttcca
      301 caaattattc atcagctaag ttcacgcaaa catgggccat acattgcagt taactgtggt
      361 gccattcccg aaggtacgat agacagcgaa ttgtttggcc atgaaaaagg atcgtttaca
      421 ggtgcccacg aagcgcgtaa aggctacttt gaaactgcca atagcggcac catattttta
      481 gacgaagttg ccgaactgcc cttaggcaca caagtacgtt tattacgtgt gttagaaacc
      541 ggtgaatttt taaaagtagg ctcttcaaaa gttcaaaaaa ccaatgtacg cgttgtagcc
      601 gccaccaata taaatatgcc gcaggcaata gccaacggta aatttcgcga agatttgttt
      661 tacaggctca acaccgtacc tatttcaatt ccatcgttgc aacaacgtaa agacgatata
      721 ccacttttat tcagaaagtt tgccagcgat tttgccgaaa agtatcgtat gagcaccaaa
      781 caattagatg ccgatgccat gcaaatgctt atagcctacc cttttccggg taacatcagg
      841 caattgcgca acttatgcga acaaatttgc atactcgaaa aaaatccggt ggttaccaca
      901 caaataccaa cacattatct gccacaggta aataccaata cattgcctgt gttgcttcgc
      961 gatgaaaaca aaaacagcca tgccgatttt gccaacgaac gcgacttgtt gtataaagta
     1021 cttttcgaaa tgaaaaaaga cattaacgac ttaaagcgtg ttgtttatga caatatgccc
     1081 gtatctgata atttcgaaaa cataccatca cctcataatg ccattattga tatgcaaggc
     1141 attatgccca caggcagtgt aacacttcaa acacatccgc aacaaatggc cgaagaagtt
     1201 gaagagtcgc tatcaatcag cgaaaaagaa aaggagctta ttgtaaaagc attacaaaaa
     1261 caccgcaacc gcagaaacaa tgctgctaaa gaattgggca taagcgagcg caccctttac
     1321 cgtaaaataa atgaatacgg tttaaaataa acctgaatgt acttggatga aaaaactaat
     1381 taacggctgg aattttagcg tattgggttt acttgttttt ttaacaagtg gctgctattc
     1441 gtttaaaagt ggccgaatac ccgataatat tgaaaccatt aatattaaat tttttccgaa
     1501 ccgtgcagcg gttgtgcaac catcgttaag ccaaatattt accgataagc ttaaagataa
     1561 atttgtatcc gaatcgcgcc tcaatttaat aactacacat gccgacctta atttcgaagg
     1621 ttatataagc gactatacca atacgcctaa tgccattcaa aactcacagc aagctgcact
     1681 aaaccggctt accattagcg tttttgtaaa gtttacaaat gcaaaagacc ctaagcaaga
     1741 ttttgaaacc acctttacac gctttgccga atacaacagc agcctgagtt ttcaaacggt
     1801 tgaaaccaat ttgattcagg aaattaaccg attgctgatt gatgatatct acaataaaag
     1861 tgtaaccaat tggtaacggc atgttatgca actataaata gcttcgcttc attaggtaca
     1921 tgtaattttt tcggtaaacc aaagggtcgt taattatgct ttcacttaaa gttcaatgta
     1981 taagcacatt acaaaaatct gttacgtttg tacaagtgtt taatgcaact aaaaatgtac
     2041 ttgcacaata aattataaag ctgattatgc atcgcaatca atttttgtcc tatatcgaaa
     2101 atcccaaaca tgccccttgg cataccgatg tgttttcgcc actattggca cagtttcctt
     2161 attgccagtc gctacatttg atgtatgcca agcacctaca ttatatcgag cattttaatt
     2221 atcaacagca actaaaactt acagccgctt atgcaggtaa tcgcagtgtg ttgcatgcat
     2281 taataaatga tccaacctat caatctgtaa caacaagttc tgttatggct gttgaacaag
     2341 agaataatgc tgccagcaac gtttttaccg aagcacctaa acatcacagc aatgtttttg
     2401 ttttagatga attagaaacc acaacagcaa caactaacaa caatacagcc caatcaattg
     2461 ctgctgccaa tgcaaccaca caatggagcg atacaaacaa tttacaacca accgatttac
     2521 cggctaatga taacttattt aatttggaag aaacggttga agaaattcag gctacggttt
     2581 tagaggaaac aactaaagat gaacttgaca gcttaacaca tttaaaatta cataacctca
     2641 acaaaaatgc tacaacgtca gataatgatt cagcagttga agttgatttg aaaaacgaaa
     2701 ttgctgatga aattatagta ccatccgatg cagtaaaagt tgaatacagc aatcaaaata
     2761 aaacgtctgc aatcacatta gaagccgaaa caaatgctga atcaactgaa gtgttaaccg
     2821 t
//
LOCUS       DAOMAA010000162         2815 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_29511_length_2815_cov_3.310870, whole genome shotgun sequence.
ACCESSION   DAOMAA010000162 DAOMAA010000000
VERSION     DAOMAA010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29511_length_2815_cov_3.310870"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2138)
                     /locus_tag="PLO59_03060"
     CDS             complement(<1..2138)
                     /locus_tag="PLO59_03060"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HQV00109.1"
                     /translation="MIRFITLLSFICITCTLFAQSGYQKIKIYASGKQLKQLAQAGVC
                     IDHGIRKTNYSFTAIFNALECEAIAASGLTYQVIEADAKVAYQNKTNQHKVQAVCGAS
                     NINTPANFALGTMGGFLTYSAMLDALDSMASKYPNLITLRMPVDTGHTIEGRPVYFVK
                     ISDNPNVAETEPEIYYSALHHAREPLSMQQLIFYMWYLLENYSSNTNVQHIVNNCQLY
                     FVPCLNPDGYLYNEFTDPSGGGLWRKNRRDNGDGSFGVDLNRNYSMFWGFDDNGSSPS
                     TSSEVYRGIGPASEPEIQMINNFINAHQFVTAFDYHSYGNLLIYPYGHIADFETPDSL
                     IFRSLAETYTAENNFLAGTPNQTVNYTSNGGSVDWLYGEQVSKPKIFDFTPECGPDFW
                     PDPSEIEDLCKQTLNMNMQLALTTLHYAHLADKSPSTLSNITGYLKYQIKNIGLDSTG
                     TFTVSVTPLTGFANVGSSKVYNLAFGQIQTDSIAYTLNVIPFSSQVTYLLQVDNGSFI
                     LSDTITKIYTPLNLIYSNDGSNLNALNALGWGVDNSNYVSAPASISDSPNQQYVPNAY
                     STLTSLPFNLTNASYGMLSFKLHLDVEPDFDYAQVQGSKDGITWIPLCGKYTKPGSIY
                     QDFGQPVYDGFTSGWLNEEISLQPLLGNNVWIRYVFVSDGFVEYDGVFLDDIEINTNV
                     TVGMPENNFNTTVLPNPAQNQITITHQGNQA"
     gene            2316..>2815
                     /locus_tag="PLO59_03065"
     CDS             2316..>2815
                     /locus_tag="PLO59_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HQV00110.1"
                     /translation="MKITVVGAGNVGATVANVCAHKELANEIVLLDIKEGIAEGKALD
                     IWQTAPINLYDSRVKGVTNDYAATAGSDVVVITSGLPRKPGMSRDDLIATNAGIVKSV
                     TENIIKHSPNTIIIVVSNPLDVMTYCAYLTAKIDASRVFGMAGILDTARYRAFLAEAL
                     NCSPKD"
BASE COUNT          871 a          547 c          555 g          842 t
ORIGIN      
        1 gcttggttgc cctggtgcgt tatggttatt tggttttggg ccgggttggg tagtaccgtt
       61 gtattgaaat tattttcagg cataccaacc gttacattgg tattaatttc aatatcatct
      121 aaaaacacac catcatattc aacaaatcca tcacttacaa atacatatcg tatccacaca
      181 ttatttccta ataagggctg caaagaaatt tcctcattca accatccgct tgtaaaacca
      241 tcataaacag gctggccaaa gtcctggtaa atcgaaccgg gtttagtgta tttgccgcaa
      301 aggggtatcc aagtgatgcc atccttactg ccttgcactt gtgcataatc aaagtcgggt
      361 tctacatcta aatgcaattt aaaactaagc ataccgtaag atgcattggt aagattaaat
      421 ggcaaactgg taagcgtact gtaggcattg ggtacatact gttggttggg cgaatcgctt
      481 atgcttgccg gtgccgatac ataattactg ttatctacac cccaacccaa agcatttaaa
      541 gcatttaaat tactgccatc attactgtaa atcaaattta atggtgtata tattttagta
      601 atggtatcac taagtataaa gctaccgtta tcaacctgta acaaataggt tacctggctg
      661 ctaaacggaa ttacattaag tgtgtatgca attgaatcgg tttgtatttg tccgaatgcc
      721 aaattgtaaa ctttgctgct gcccacgttt gcaaaaccgg ttagaggtgt aacacttact
      781 gtaaatgtgc cggttgaatc taaaccaatg tttttaattt ggtatttcaa atagccggta
      841 atgttgctta atgttgatgg gcttttatcg gcaagatgtg cataatgcaa agttgtaagg
      901 gctaattgca tattcatgtt taaggtttgt ttgcacaaat cttcaatttc agatgggtcg
      961 ggccaaaaat cggggccaca ctccggtgta aaatcaaata ttttgggctt acttacctgc
     1021 tcgccataca accaatctac actgccaccg tttgatgtgt agttaacggt ttgattgggt
     1081 gtgcctgcta aaaaattatt ttcagcagta taggtttcgg ccaggctgcg aaatataagc
     1141 gagtcgggcg tttcaaaatc ggcaatatga ccatagggat aaataagcaa attgccatag
     1201 ctgtgataat caaaagcagt tacaaactga tgtgcattta taaagttatt aatcatctgt
     1261 atttcgggtt cgctggccgg cccgatgcct ctataaactt cgctcgatgt actgggcgat
     1321 gagccattat catcaaaacc ccaaaacata ctatagttac ggtttaagtc aacaccaaaa
     1381 gaaccatcgc cattatctct gcggttttta cgccacaaac caccaccact tgggtcggta
     1441 aattcattat acaaataacc atcaggattt aaacagggta caaaatacaa ctgacaatta
     1501 tttacgatgt gttgtacgtt ggtgttcgaa ctataatttt cgagcaggta ccacatataa
     1561 aaaataagtt gttgcatgct taatggttcg cgtgcatgat gcaaagccga gtaataaatt
     1621 tcaggttcgg tttcggccac attcggatta tcacttatct ttacaaaata aacaggtcgt
     1681 ccttcaatgg tatggcccgt atcaacaggc atacgcaggg taattaagtt tggatatttt
     1741 gatgccatgc tgtctaatgc atctaacatg gcactgtatg ttaaaaaacc acccattgtg
     1801 cccagagcaa aattggcagg tgtattaata ttgcttgcac cacatacggc ctgcacctta
     1861 tgttgattgg ttttattttg ataagcaact tttgcatcgg cttcaataac ttgataagtt
     1921 aaaccactgg ctgcaatggc ctcacattca agagcattaa aaatggccgt aaaactgtag
     1981 ttggttttac gtatgccgtg gtcaatacaa acaccggctt gtgccaattg tttcaattgc
     2041 ttgcctgatg cataaatttt aattttttga tagccacttt gtgcaaacaa tgtgcaggta
     2101 atacaaataa agcttaggag tgtaataaaa cgaatcatgt tgatttatat aagcgctgct
     2161 aagtaattac aaattgtttt tgaataagca taccaaagtc agctattggt aatcaaacaa
     2221 aaattagctt ttgtaaatgt gtagcaattt atactttcgg ccgcccttaa aacagggata
     2281 caataattgt tttataaatt aaataccaaa taaaaatgaa aattacagta gtaggtgcag
     2341 gcaatgtagg cgccaccgtt gccaatgttt gtgcgcataa agaattggca aacgaaattg
     2401 ttttactgga tattaaagaa ggcattgccg aaggcaaagc gctggatatc tggcaaacgg
     2461 cacccatcaa tttatacgac tcacgcgtaa aaggtgtaac caacgattat gctgcaacag
     2521 ctggcagcga tgtagttgtt ataacatcag gcttaccacg taaaccgggc atgagccgcg
     2581 atgatcttat agctaccaat gcaggcatcg taaaatcagt taccgaaaac attatcaaac
     2641 attcgccaaa taccatcatc atagtggttt caaatccatt agatgtaatg acgtactgtg
     2701 cataccttac agctaaaata gatgcaagcc gcgtatttgg tatggcaggc attttggata
     2761 cagcgcgcta tcgtgcattt ttagccgaag cattaaactg ctcgcctaaa gacat
//
LOCUS       DAOMAA010000163         2807 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_29671_length_2807_cov_2.785974, whole genome shotgun sequence.
ACCESSION   DAOMAA010000163 DAOMAA010000000
VERSION     DAOMAA010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29671_length_2807_cov_2.785974"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1718)
                     /locus_tag="PLO59_03070"
     CDS             complement(<1..1718)
                     /locus_tag="PLO59_03070"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HQV00111.1"
                     /translation="MKKVLLLLTTLFAYHFSLGCDTTAISKTLWSVAFVDSEELTGEG
                     PNNGHAVHCIDNDSATFWHTQWQGAAPPYPHEIQINLGAVYPVNGFSFLTRANSTNGR
                     VKSFTFYLSTDGINWGSAQAAGDLVYPNPTSNAQQAAAVYFGAVSAQYIRLVANTSYA
                     GVYAMCAELNVYQDSSCGATGQTNQVITINPIATQTTVSAPVVLSGVSTSGLPLSYSV
                     VSGPASIQGDTLTVTGVAGVVVVEAYHNGNTTYYPAAAQNTFTVLDLTTYYPDLNCKL
                     TDSLDLQMPQLYPYLLHATANIVHPQFLSVSSVTYSINGVNLTALENSGDYQAWWTPP
                     AYGNYTIYVTATASNGKTDVDTINLNVSQTITTQNVQTFTDAVIDLGTIGSQWYYGTY
                     QLPQSVGAYDSIVANLHISCPSVPGACDDWDRIAWVEIKGPDGKWMELFRYITPYGVA
                     CNHQIDVTDYASVLQGNIEFRMYIETWGTGGWKINLDFDYYAGTPDYLYSAIEKIWHG
                     TYSFGDPNKLQPMDTVMVKPYPNVGKATFRLVTTGHGWGNNNTGNAAEFYHAIHNLQI
                     DNVNTFV"
     gene            1942..>2807
                     /locus_tag="PLO59_03075"
     CDS             1942..>2807
                     /locus_tag="PLO59_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00112.1"
                     /translation="MSKSNLISVIILLTSIHLYAQKKQVLFLGNSYTAVNNLPKMIAD
                     IALSTGDTLIFDSNTPGGYTLQGHSTNSTSLSKIMLGNWDNVILQEQSQLPSFGNNQV
                     QLQVFPYARLLDSLINQYNPCAETMFYMTWGRKNGDAANCAVFPIVCTYNGMDSMLNL
                     RYRMMADSNQAVVSPVGAVWKYIRQNFPLIELYQADESHPSLAGSYAAACCFYAAIFR
                     KNPALITDNYSLPATDADNIKAAAKAIVYDSLQNWHIGEYDPLADFNFNINNNTVTFN
                     NNSVNATTYQWT"
BASE COUNT          861 a          572 c          542 g          832 t
ORIGIN      
        1 acaaaagtgt ttacattatc tatttgcaaa ttatgaatgg catgataaaa ctcggccgca
       61 ttacccgtat tattattacc ccacccatgc cccgttgtta ccaaacggaa agtagccttg
      121 cccacatttg gataaggctt aaccataaca gtatccatag gctgcaattt atttgggtcg
      181 ccaaatgaat aggtgccatg ccagattttt tcgattgcag aatacaaata atcgggtgtg
      241 ccggcatagt aatcaaaatc aagattaatt ttccaaccac cggtgcccca ggtttcaatg
      301 tacatccgaa actcgatatt gccttgaagt accgatgcat aatcggtaac atcaatttga
      361 tgattacatg ccacaccata gggcgtaatg tatctgaaaa gttccatcca cttgccatcg
      421 gggcctttaa tttcaaccca tgcaatacgg tcccaatcat cgcaggcacc gggcactgat
      481 gggcatgaaa tatgaaggtt tgccacaata ctatcgtaag cgccaaccga ttgtggcagt
      541 tggtaagtac catagtacca ctgcgaacct atggtcccta aatcaataac ggcatcggta
      601 aatgtttgta cattttgtgt tgttatggtt tgactcacat tgaggttaat ggtatcaaca
      661 tcggttttgc cattgctggc agttgctgtt acgtatatgg tgtagttacc ataagccggt
      721 ggtgtccacc aggcttggta atcgccactg ttttctaaag cagttaggtt aacaccattt
      781 atagagtaag taacggatga cactgacaag aactgaggat gaacaatatt agccgtagca
      841 tgtaataaat agggatagag ttgcggcatt tgcaaatcga gtgaatcggt tagtttgcaa
      901 tttaaatcag gatagtaagt ggttaaatct aatactgtaa aagtattttg tgctgcggcc
      961 ggataatagg ttgtattgcc attgtgataa gcttcaacaa caacaacacc ggcaacacct
     1021 gttacagtta aggtgtcgcc ttgtatgctt gccggccccg aaacaacgct atacgaaagt
     1081 ggcaatcctg aagtagaaac acctgataaa acaacaggtg ccgatacggt tgtttgtgtt
     1141 gcaatagggt tgatagtaat aacttgattg gtttgaccgg tggcgccaca actcgagtcc
     1201 tgatacacat ttaactcagc acacatggca tatacaccag cgtaacttgt attagcaact
     1261 aatcgaatgt attgtgcact aacagcgcca aaataaacgg ctgctgcttg ctgtgcattg
     1321 cttgttgggt tgggataaac caaatcaccg gctgcttgtg cactacccca attaatacca
     1381 tcggtactta aataaaatgt aaagcttttt accctgccat tggtactatt ggcacgggtt
     1441 aaaaaactaa acccgtttac aggatacact gcacccaggt ttatttgaat ttcatgcggg
     1501 tatggtggtg ccgccccctg ccattgtgta tgccaaaaag ttgccgagtc gttatcaata
     1561 caatgtactg catggccatt gtttggtccc tcaccggtca attcctcact atctacaaag
     1621 gcaacactcc atagtgtttt cgaaattgca gttgtatcac aaccaaggct aaagtgatat
     1681 gcaaataaag tagttaacag tagtaatact tttttcattt tattattgtt gttgataatt
     1741 acattcccaa tgtagcggca aatttagcag ccgaagccgt ttataaaatt acttccataa
     1801 aaattgacta aattataata cttcaacgtg tattattgat ttaaacttgc agcccaataa
     1861 aagcataaat tattttaaac ataatttaca attacaagca acaacagctc atgcttgtta
     1921 taaaccattt cgaaaaataa aatgtcaaaa tcaaacctta tatccgtaat catacttcta
     1981 acatcaatac acttatatgc tcaaaaaaag caagtacttt ttttgggcaa cagctatact
     2041 gctgtaaata atttgccaaa aatgattgcc gatattgcct tatcaaccgg agatactttg
     2101 atttttgata gcaatacacc gggcggatat acattgcaag gccattcaac caatagtact
     2161 tcgcttagta aaataatgtt gggcaattgg gacaatgtaa tactgcaaga gcaaagtcaa
     2221 ctgccctcat ttggtaacaa tcaggtacag ctgcaagttt ttccatacgc acgtttatta
     2281 gatagtttaa taaatcaata taacccctgt gccgaaacca tgttttatat gacttggggc
     2341 cgtaaaaatg gcgatgctgc taattgtgca gtatttccaa ttgtatgtac ttataacggt
     2401 atggatagca tgcttaattt gcgttaccgc atgatggccg acagcaatca agccgttgtg
     2461 tcacctgtgg gtgctgtttg gaaatatatc aggcaaaatt ttccgcttat cgaattgtat
     2521 caggctgacg agagtcatcc atcgcttgca ggttcttatg ctgctgcttg ttgtttttat
     2581 gcagccatat ttcgcaaaaa tccggcacta ataactgata actattcgtt gcctgcaacc
     2641 gatgctgata atattaaagc agccgcaaaa gcaattgtat atgacagtct tcaaaattgg
     2701 catattggtg aatatgaccc cttggctgat tttaatttta atataaataa caatactgtt
     2761 acctttaaca acaactccgt aaacgcaact acttatcagt ggacttt
//
LOCUS       DAOMAA010000164         2804 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_29722_length_2804_cov_2.512550, whole genome shotgun sequence.
ACCESSION   DAOMAA010000164 DAOMAA010000000
VERSION     DAOMAA010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29722_length_2804_cov_2.512550"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="PLO59_03080"
     CDS             <1..876
                     /locus_tag="PLO59_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013634063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HQV00113.1"
                     /translation="HLVILDSIQTLFTGKIESAPGSVSQIRSCAAELMRYAKETDTPV
                     MIIGHITKDGSIAGPKVLEHMVDTVLHFEGDRNHVYRILRAQKNRFGSTAELGIYEML
                     SNGLRQVSNPSEILLTQRNEQYSGISIACTMEGMRPILIEIQALVSSAVYGMPQRSAT
                     GFDLRRLGMLLAVLEKRCGFKLGAKDVFLNIAGGIKVEDPAMDLAVICAVLSSNDDMP
                     LPTKVCFAGEVGLSGEIRPVSRIEQRIKEAEKLGFSRFFFSKYQMKISGLQKNTQIEL
                     VPVAKVDEVFAQMFG"
     gene            996..2003
                     /locus_tag="PLO59_03085"
     CDS             996..2003
                     /locus_tag="PLO59_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00114.1"
                     /translation="MKKTLLLFACWFCISNATAQNFEMGIAPGFTNYFGDLGNNEFFQ
                     NTSTRPAVALTLRNFLGKSKISTNHYKSFDMEARFSWQRIAYDETKPINGRQGDDLRN
                     YLRGLSFRTDMFSLESRVTYTLYKNKKQPLHIQKSCMYFFTGLALVYGNPKADLFNGS
                     IDLNNRYYFWSDGSVRNAPESSGTGEIIERDGTYETELNDWHTEGESQKTGIGKNGFL
                     SCTNVAVPLGLGFRFGVSKKINVGFEVAYYKMFTDAIDNVSDRYVYQTELENYFPNDI
                     NQQQLAAYISDPTGNGTNGYPGPQTSQRGNPSKNDSYTCISLELSYKFDFLAKKVFGM
                     R"
     gene            2122..>2804
                     /locus_tag="PLO59_03090"
     CDS             2122..>2804
                     /locus_tag="PLO59_03090"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HQV00115.1"
                     /translation="MRIYNLLLFGFFLSINVSAYQITGTIYDENKQVMPFVSVFIKGT
                     TQGTTSNSEGKYQIEVTAGNVTLIYKLIGYKQFEKTYTVTVTNIQQDVYMVPEHYELK
                     EAIVKGNEDPAYEIIRQAIIKRRFYLEQVQAYKADVYIKGWQRITKHPEKIFGQKVDL
                     SEVLDTATGVVYLSESVSSFNFKRPNQIRETIKSSKVSGSNRSFSFNRASFFLFNFYE
                     NIMEISLCPR"
BASE COUNT          920 a          523 c          574 g          787 t
ORIGIN      
        1 cacttggtaa ttcttgattc gatacaaact ttgtttacag gtaaaattga atcggcaccg
       61 ggcagcgtat cacaaatacg aagctgtgca gcagagttga tgcgatatgc caaagaaacc
      121 gatactccgg taatgattat tggccacata actaaagatg gcagtattgc cggacctaag
      181 gttttggagc acatggttga tacggtatta catttcgaag gcgaccgcaa tcatgtatat
      241 agaattttac gtgcgcaaaa aaatcgtttt ggctcaactg ccgaattggg catttacgaa
      301 atgctaagca acgggctgag gcaggtatca aaccccagcg aaatattact aacgcaacgt
      361 aatgagcagt acagtggcat tagcattgcc tgtactatgg aaggtatgcg ccccatttta
      421 atcgaaattc aggcattggt tagcagtgcc gtatatggca tgccgcaacg cagtgccacc
      481 ggttttgact tgcgcaggtt aggcatgttg ttggccgttt tagaaaaacg atgcggcttt
      541 aagttgggtg ctaaagatgt gtttttgaat atagccggtg gtattaaagt agaagatccg
      601 gcaatggacc tggcagtaat atgtgccgtg ttaagcagca atgacgatat gcctttgcca
      661 actaaagttt gctttgcagg cgaagtagga ctaagtggcg aaataagacc tgtaagccgt
      721 attgaacaac gtattaaaga agccgaaaaa ttaggcttta gtcgtttctt cttttcgaaa
      781 taccaaatga aaattagtgg cttgcaaaaa aacacacaaa tagaactagt acctgttgcc
      841 aaggttgacg aagtatttgc acaaatgttc ggctaatatt tgtaatcaac tttacagggt
      901 aattaaataa tttgtattat tgaaagtatt aagcaccccg taacaaactg ctgcctgcgt
      961 atatggcaat agataccaac ttaaaattta cggtcatgaa aaaaacatta cttctttttg
     1021 catgctggtt ttgcatttcg aatgcaactg cacaaaactt tgaaatgggc attgcgcccg
     1081 gttttacaaa ttactttggc gatttgggta ataacgaatt ttttcaaaat acatcaacac
     1141 ggcctgctgt tgctttaacg ttgcgcaatt ttttgggcaa gtcgaaaatc agcaccaatc
     1201 attataaaag ttttgatatg gaagcacgct ttagctggca acgcatagcc tatgacgaaa
     1261 ccaaaccaat aaatggccgg cagggcgatg atttacgcaa ttatttgcgg ggccttagtt
     1321 tcagaaccga tatgttttcg ctcgaaagca gagttacata cacgctttat aaaaacaaaa
     1381 aacaaccatt acacattcaa aaatcttgca tgtacttttt tacaggcctt gcattggttt
     1441 atggcaatcc caaagccgat ttgtttaatg gctcaattga tttaaacaat cgttattatt
     1501 tttggagtga tggcagtgtg cgtaacgcac ccgaaagtag tggcaccggt gaaattatag
     1561 aacgcgatgg cacttacgaa accgaattaa acgattggca cactgaaggc gaaagtcaaa
     1621 aaaccggcat tggcaaaaat ggatttttaa gttgcaccaa tgtagccgtt ccgttaggat
     1681 tgggatttag gtttggcgta agcaaaaaaa ttaatgtggg ttttgaagtt gcctattata
     1741 aaatgtttac cgatgccatt gataatgtga gcgaccgata tgtgtatcaa accgaattag
     1801 aaaattattt tccgaacgat ataaaccaac aacaattggc tgcttatata agcgacccaa
     1861 ccggtaatgg caccaacggt tatcccgggc cgcaaaccag tcaacgcggc aatccatcaa
     1921 aaaacgacag ttatacctgc atcagtttgg aactatcgta caaatttgat tttcttgcca
     1981 aaaaagtttt tggcatgcgt tagttgaaca atcacttctg atcaaactaa agttcattaa
     2041 aaaatatatg aaccttttaa aagtggtgta actatcaatt acaatttacc gtcaaatgcc
     2101 atataactgc aaaaagaatt tatgcgcatt tacaacttac tcctgtttgg ttttttttta
     2161 agtatcaacg ttagtgctta tcagattacc ggcaccattt acgatgaaaa caaacaagta
     2221 atgccttttg taagtgtttt tattaaaggc accacacaag gcaccaccag caacagtgaa
     2281 ggtaaatatc aaatagaagt tacagcaggt aatgttactt taatttataa actcattggc
     2341 tataaacagt tcgaaaaaac ctatacagta actgttacta acatacaaca agatgtgtat
     2401 atggtgcccg aacattatga attaaaagaa gccattgtaa aaggcaatga agatccggcc
     2461 tacgaaatta tcagacaagc tataatcaag cggaggtttt atttagagca ggtgcaagca
     2521 tacaaagccg atgtgtatat aaaaggctgg caacgcataa ccaaacatcc cgaaaaaata
     2581 ttcggtcaaa aagtagatct ctccgaagtg ctcgataccg ctacaggcgt ggtttattta
     2641 agcgaaagcg tatcgagttt taattttaag cgccccaatc aaatacgcga aaccattaaa
     2701 tcgagtaaag ttagtggcag taatcgctca tttagtttta accgggccag tttttttctg
     2761 ttcaattttt atgagaacat aatggaaata agcctatgcc cgcg
//
LOCUS       DAOMAA010000165         2793 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_29956_length_2793_cov_2.758948, whole genome shotgun sequence.
ACCESSION   DAOMAA010000165 DAOMAA010000000
VERSION     DAOMAA010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29956_length_2793_cov_2.758948"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="PLO59_03095"
     CDS             complement(<1..612)
                     /locus_tag="PLO59_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00116.1"
                     /translation="MKKLIVLLLFFSSALFAQDTIFKKDQTKIITKVVEVNQDDIKFK
                     DLDIADGPMFTMEKSEIVRIVFSSGKEVRFNPDPYSVSGDVEVRDKTKAIKFEFFSPL
                     TDNITFCYEFMYRVGLNIEAKVALIGLGVADYPKASGAFFKVGPKFLLGSDYVSGNTK
                     YAHGLRGRYFKPEITVSIYTREEGGVSSFGGTTKRDEVRFASYG"
     gene            complement(643..2265)
                     /locus_tag="PLO59_03100"
     CDS             complement(643..2265)
                     /locus_tag="PLO59_03100"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020211544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HQV00117.1"
                     /translation="MKNIYLVLLLFANLTQAQLFDTHSLTHADTLRGMLTPVRTCFDV
                     TYYYLNVKVDVDSQQIEGVNTINFNVLASTDSIQIDLFEHYKIKSIVHNDQFLDYTRD
                     GNAVFVKFKKTLQQGSRQTIYFSYQGKPNIAKNPPWDGGFTFKKDSSGMPWVGVSCQG
                     FGASCWWPCKDHQSDEPDSMTIAITCPSNLINVSNGRLRKKEANQKGYMRYEWFVANP
                     INTYNVTLNIANYAHWHDTLDALTLDYYVLRENEVKARKQFEQVKPMLTCFEKYFGKY
                     PWYSDGYKLVETPYLGMEHQSAVAYGNQYKNGYLGRSLSTSGIGKKFDYIIIHETAHE
                     WWGNNITTKDIADMWVHEGFTVYAEALYCECLWGYDDYIKYINGMKFNIQNDKPVIGL
                     YDVNTEGSGDMYNKGAMLVHTLRSIFNDDEKFLGLLKKIQKDFYHQTVTTKQIEDYFT
                     KAYGKDLQPVFDQYLRNKSIPKLALQLQDGVVKYRWQCDVAAFNMPVKIKAADSYVWI
                     EPTTQWQTLKGYSNVDDVKVATELFYIKVIKP"
     gene            2481..>2793
                     /locus_tag="PLO59_03105"
     CDS             2481..>2793
                     /locus_tag="PLO59_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00118.1"
                     /translation="MEVLDNDYNNSNQSNKQTGNNTYMPNAGNQNNKIIGGFIVVVIG
                     LIFLLKQMGFYLPYWLFKWEIILIGIGLFIGFKSDFQNISWFILVLIGGLFLVGDILP
                     DM"
BASE COUNT          849 a          526 c          479 g          939 t
ORIGIN      
        1 accataactg gcaaagcgta cttcatcacg tttggtggta ccgccaaacg aacttacacc
       61 gccttcttct ctggtatata tactaacggt aatttcgggt ttaaaataac ggccccgcaa
      121 accatgtgca tatttggtat tacccgaaac ataatcgctg ccgagcaaaa atttggggcc
      181 tactttaaaa aaggcaccac tggcttttgg ataatcggca acacccaaac caatgagggc
      241 tactttggct tcgatattaa gtccaacccg atacataaat tcataacaaa atgttatgtt
      301 atcggttaag ggcgaaaaaa attcgaactt gattgctttg gttttatcgc gtacttcaac
      361 atcgccactt acgctgtatg ggtcaggatt aaacctaact tcttttccgc ttgaaaaaac
      421 aattcgtaca atttctgatt tttccattgt aaacataggg ccatcagcaa tatctaaatc
      481 tttaaacttt atatcgtctt gatttacttc taccactttg gtaataattt ttgtttgatc
      541 ttttttaaag atggtatcct gtgcaaacaa tgcactgctg aaaaacaaca ataatactat
      601 aagctttttc atcggtttaa atttatagtt caaatatagt ttttaaggct ttataacctt
      661 tatataaaat aattcggtag ctactttaac atcatcaacg tttgaataac cttttaaagt
      721 ttgccattgt gttgtaggct caatccaaac ataactatcg gccgctttaa tttttacagg
      781 catattaaat gcagccacat cgcattgcca tctgtattta actacaccat cttgtaattg
      841 caatgccaat tttggaatag atttattacg caaatactga tcgaataccg gttgcaaatc
      901 tttaccgtaa gcctttgtaa aatagtcttc aatttgtttg gtagtaaccg tttgatggta
      961 aaaatctttt tgtatttttt ttagcagccc taaaaacttt tcatcgtcat taaaaatact
     1021 gcgtaacgta tgaaccaaca tggcgccttt attatacata tcgccactgc cttcggtgtt
     1081 tacatcatac agaccaataa caggtttatc gttttgtatg ttaaacttca tgccgttaat
     1141 gtatttaata tagtcgtcat agccccataa acattcgcaa tacaaggctt cggcataaac
     1201 tgtaaaacct tcgtgcaccc acatatcggc aatatctttg gtagtaatgt tattgcccca
     1261 ccattcatgt gctgtttcat gtatgataat gtaatcaaac tttttaccaa tgcctgatgt
     1321 tgagaggctg cgccctaagt atccgttttt atactgattg ccataggcta cggcactttg
     1381 atgctccata cccaaatatg gtgtttcaac caatttataa ccatcgctat accagggata
     1441 tttaccaaaa tatttttcga aacaggtaag catgggtttt acctgttcga attgtttacg
     1501 cgctttaact tcgttttcgc gcagtacgta gtagtcaagc gttagtgcat ccaaggtgtc
     1561 gtgccagtgt gcatagtttg caatattcaa ggttacatta taagtgttta tcggatttgc
     1621 cacaaaccat tcgtatcgca tgtagccttt ttgattggct tcctttttac gtaggcggcc
     1681 gtttgataca tttattaaat tagacggaca tgtaatggct atggtcatac tatcgggctc
     1741 atcgctttga tggtctttac aaggccacca gcaactggca ccaaaaccct gacatgatac
     1801 accaacccag ggcataccac tgctgtcttt tttaaacgta aaacccccat cccagggtgg
     1861 gtttttagca atgttgggtt tgccctgata cgagaagtaa atagtttgtc tgctgccttg
     1921 ctgcaatgtt tttttaaact ttacaaaaac agcattgcca tctctggtat aatctaaaaa
     1981 ctgatcgttg tgtacaatac ttttaatttt ataatgttcg aataaatcaa tttgaatact
     2041 atcggttgat gccaacacat taaaattaat ggtatttacg ccttctatct gttgcgagtc
     2101 aacgtcaacc tttacattta aatagtagta ggtaacatca aaacaagtac gcaccggagt
     2161 aagcataccg cgcaaagtat cggcatgtgt taggctgtgt gtatcgaata attgtgcttg
     2221 ggttaggttt gcaaacagca gcaaaaccaa ataaatgttt ttcatagttt ataattaacg
     2281 cctttaaagt gccattaaat agtatggtaa aataataagt gtgttctatt aactcaaaat
     2341 taaaaaagat gaattcatat tttatcgttt accgatggtt tatgttgttt accgacaacg
     2401 catgtcattt gccgatttac tgttggattg cttgcagcgt gaaaatatgt ttgtacccat
     2461 aaattaaaaa ccaattaaaa atggaagtat tagataacga ttacaataac agtaatcaaa
     2521 gtaacaaaca aacaggcaac aatacataca tgcctaatgc cggcaaccaa aacaacaaaa
     2581 taataggtgg ttttattgta gttgttattg gcttaatatt tctactaaaa caaatgggct
     2641 tttatttacc ttattggtta tttaagtggg aaattatatt gattggtatc ggattgttta
     2701 ttggatttaa aagcgatttt caaaacatca gttggtttat attggtattg attggtggat
     2761 tattcttagt aggcgatatt ttgcctgaca tgc
//
LOCUS       DAOMAA010000166         2789 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_30024_length_2789_cov_3.031822, whole genome shotgun sequence.
ACCESSION   DAOMAA010000166 DAOMAA010000000
VERSION     DAOMAA010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30024_length_2789_cov_3.031822"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..1672)
                     /locus_tag="PLO59_03110"
     CDS             complement(143..1672)
                     /locus_tag="PLO59_03110"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV00119.1"
                     /translation="MKLQSKNIFKSLLVLLISATTSVHNVKAQGIFRCPLEETSGNHF
                     SELFVINRVPYHINNLGYVLDYHCTPYKSDLGITATHFELVPAGRQYVSTPIARVVAA
                     AAGQIIDKYNSYDDLTCNGSSPTGNYIQIQHTNGMTTKYSFLRKNSLTNKSIGDFVDE
                     GEFLGYPGNQSALNGYNNAQYGGFAFEVKNSSNIFIDPFDMSDSICFYHQRISLWKNP
                     SYMDDKVWKSRMLAVDLDNNFITDSCGSYPDRFKRHFNYGDLVDVIVKLANADDSLLL
                     NIYNPVGVPITSTMGEDFQFYYLMDRTAFVKFDVPLTGVMIIPGTYTVDCIWYDSYTG
                     YQTIKQTMRQYFTVGCVPDYTLTGTVNNHQGCIAGNNIYSSEVCNSGSRVNYIADNEI
                     ILNPGFKAYNGSDVFIYTDPCVATPRLENQVDIENENQSTELNIFPNPANDFITIKTN
                     SDSQTILKIYNSTMQLMKTFNFENGIENQISTADLSPGIYFIDAQSAGQTHKAKFIVN
                     H"
     gene            complement(1685..>2789)
                     /locus_tag="PLO59_03115"
     CDS             complement(1685..>2789)
                     /locus_tag="PLO59_03115"
                     /inference="COORDINATES: protein motif:HMM:NF013580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HQV00120.1"
                     /translation="TGPCKFLEIATEADFEYYQDFSSNVNTALNRIRTILNQVEGLYT
                     GTFKVKFVLRYQQIWTTAADPYSGDTLQGILPQFTNWWNANRTSINRDVTHMFTGRSI
                     SNTTVGFAWIGVVCDANWYYSTVTNFSPVTMSTINLSTHELGHNFGAGHDITCNPSSI
                     MCPNIQSGSVGWAATSITEINSHLNTISCLDESFPNSITFPSPGFPISYAKVLAINSI
                     AINSALGPVEISNSGFAHLEAGNEIVLSPTVFNTTGFIANEGSAVLLRISDMVNTCLP
                     LRLMPDNEVHITAADLSIFPNPANEFITVKLTSDAQTILKIYNSTMQLLKTIPFENGL
                     ENQISTADLSTGIYFIDAQSGNQSYKTKLVVNR"
BASE COUNT          842 a          512 c          529 g          906 t
ORIGIN      
        1 tgaatacgat ttaaccgtta aatttgaatt tactaaaaag gcaaagcatg ttaacctgaa
       61 tacattttac ccttaaaagg cataaaaaaa cggaagcata aaaattaata cacttccgtt
      121 ttaaaataca gtttaaataa ttttagtggt taacaataaa cttggcttta tgagtttggc
      181 cagcgctttg cgcatctata aagtatatac cgggcgataa atcagcagta gaaatttgat
      241 tttcaattcc attttcgaaa ttgaaggttt tcatcaactg catggtgctg ttgtaaattt
      301 tcaatatggt ttgtgaatca ctgtttgttt tgattgtgat gaagtcattt gcaggatttg
      361 ggaatatgtt taattccgtt gattggtttt cattttcaat atcaacctga ttttccaatc
      421 ttggtgtcgc cacacaaggg tcagtataaa taaacacatc actaccattg taagccttaa
      481 agccgggatt aagtataatt tcattatctg cgatataatt aactctcgaa ccgctattgc
      541 atacctcgga gctgtaaata ttgtttccgg caatacaacc ctgatgatta tttaccgttc
      601 cggttaaggt atagtcgggt acacaaccta ctgtaaaata ctgccgcatg gtttgtttaa
      661 tagtttgata gcctgtgtag ctatcgtacc atatacagtc aacagtgtat gtccccggta
      721 tgatcattac acctgttaat ggcacatcaa acttgacgaa tgcagttctg tccataagat
      781 aataaaattg aaaatcctct cccattgttg aagttatagg aacaccgacc ggattataga
      841 tatttaaaag taatgaatca tctgcattgg caagtttgac tattacgtca actaagtctc
      901 cataattaaa atggcgctta aacctgtcag ggtaactgcc gcagctatca gtgataaagt
      961 tattatctaa atcaacagct aacatgcgtg atttccaaac cttgtcatcc atatatgagg
     1021 gatttttcca taaagaaatt cgttgatgat aaaagcaaat agaatcactc atatcaaatg
     1081 ggtcgataaa tatattactg ctatttttca cttcgaatgc aaaacctcca tactgcgcat
     1141 tattgtaacc gtttaatgcg gattgattgc cgggataacc caagaattcg ccttcatcta
     1201 caaaatcacc aattgattta ttcgtcaaac tgttttttct caagaatgaa tacttggtag
     1261 tcattccgtt cgtatgttga atttgaatgt aattacctgt tggactactg ccattacaag
     1321 ttaaatcatc gtatgaatta tacttgtcaa ttatttgtcc agcggctgca gctaccaccc
     1381 gtgctattgg cgtgcttaca tattgccttc cggcaggcac caattcaaaa tgagttgctg
     1441 ttattcctaa gtcagattta taaggtgtgc agtggtagtc aagtacataa cccaaattat
     1501 taatgtgata gggcacgcgg tttattacaa ataactcact gaaatgattg cctgaggttt
     1561 cttctaaagg gcaacggaaa ataccttgtg cttttacatt atgaacactt gttgttgcgg
     1621 atataagcaa aacaagcaac gatttgaaaa tattcttgga ctgtagtttc attttatatt
     1681 tgttttatct gttaaccaca agtttcgttt tataggattg atttcctgat tgtgcatcaa
     1741 taaagtaaat gcccgtagat aaatcagccg tagagatttg attttccagc ccattttcaa
     1801 atgggattgt tttcagtaac tgcatggtgc tgttgtagat tttcaaaatg gtttgtgcat
     1861 cgcttgttaa ttttacagtg atgaattcgt tggcggggtt ggggaaaatg cttaaatcag
     1921 ctgcggttat atggacttcg ttatctggca ttaatctaag gggcaagcag gtattcacca
     1981 tatcgctgat tcggagtaat acagcgcttc cttcattggc aataaatcct gttgtattaa
     2041 atacagttgg acttaacacg atttcattcc cggcctccaa gtgtgcaaaa ccactgttgc
     2101 ttatttcaac cgggccgagc gcggaattaa tagcaattga attaatagca agcactttcg
     2161 catagctaat tggaaacccc ggacttggaa aggtaattga attggggaaa gactcatcga
     2221 ggcaactaat ggtattgaga tgcgaattaa tttcagttat tgaagttgcg gcccatccta
     2281 ctgaaccaga ttggatatta ggacacataa tacttgatgg attacaggta atatcgtggc
     2341 cagctccaaa attatgccct aactcatgag ttgatagatt gatggtactc atcgtaacgg
     2401 gagagaagtt agtgacggta ctgtaatacc agttggcgtc acaaacaacg ccaatccacg
     2461 caaatccaac agttgtattg cttattgagc ggccggtaaa catgtgagta acatccctgt
     2521 taattgatgt tctatttgca ttccaccaat ttgtaaactg tggtaatatt ccttgtaagg
     2581 tatcaccact gtaagggtcc gctgctgttg tccatatttg ctgataacgc aacacgaatt
     2641 taaccttaaa tgttcctgta taaaggcctt ctacctggtt caaaatagtt ctgattctat
     2701 ttagcgcagt gtttacattg gaactaaaat cctggtaata ttcaaagtca gcttcggtag
     2761 caatttctaa aaatttacac ggccctgtt
//
LOCUS       DAOMAA010000167         2785 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_30114_length_2785_cov_2.993407, whole genome shotgun sequence.
ACCESSION   DAOMAA010000167 DAOMAA010000000
VERSION     DAOMAA010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30114_length_2785_cov_2.993407"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>289)
                     /locus_tag="PLO59_03120"
     CDS             complement(<1..>289)
                     /locus_tag="PLO59_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008682621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoglutarate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HQV00121.1"
                     /translation="NHEFMVRMREAKVNKVADSIPLQNIDNGNESGKLLVLGWGSTYG
                     AIKSAVIAARAQGLDVSHAHVRYMKPFPKNLGSLVAGFDKVLIPEMNTGQL"
     assembly_gap    290..299
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<300..1729)
                     /locus_tag="PLO59_03125"
     CDS             complement(<300..1729)
                     /locus_tag="PLO59_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HQV00122.1"
                     /translation="MENTTEVLEQVIIKFAGDSGDGMQLTGTQFTTTAALLGNDLSTF
                     PDFPAEIRAPIGTLAGVSGYQLHFGSVNIYTPGDTCDVLVAMNAAALKANIKGLKKGG
                     TIIANIDGFDPKNLRLANVAVNPLDDNSLDGFRLIKIDVTKLTRAALSESGMGTKEID
                     RSKNMFVLGFLYWMYSRSLDASIKFLTEKFSKKPEILDANIRILKAGYAYGDTTETII
                     TRYHIGAAPMKPGTYRSIMGNQATALGLIAATQQSGLPMFYGTYPITPASDILHELAK
                     YKNMNVRTFQAEDEIAAICATIGAAFGGALAVTASSGPGIALKGEAMGLGVMLEMPLV
                     IINVQRGGPSTGLPTKTEQSDLLQALHGRNGEAPMPIVAACTPSDCFTMVLEACRIAI
                     EYMTPVMFLSDGYIANGAEPRRFPTQADLPKIKVDFAKDKNGGEKYLPYKRDDKLSRP
                     WAIPGTPGLEHRIGGLEKQHETGNIS"
     gene            2002..>2785
                     /gene="era"
                     /locus_tag="PLO59_03130"
     CDS             2002..>2785
                     /gene="era"
                     /locus_tag="PLO59_03130"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HQV00123.1"
                     /translation="MMHKAGFVNIIGKPNVGKSTLLNVLLGENLAIVNPKAQTTRHRI
                     KGVLNGTDYQIVFSDTPGIMKPAYKLHHKMLDAVNETFTDADVMIYVAEIGETEMDDG
                     LKEKLKTLKVPVFLVLNKMDTINQEQLENALQHWKSVFDFTTIIPISALHKFNTAFII
                     ENIIKHLPFADAYYDKETLTDRNERFFVTEILREKILSLYQKEIPYSTEVAIVYFKDE
                     PNISKIGTVIYVERESQKAIVLGHQGKAIKRLGTEARKSMETF"
BASE COUNT          831 a          571 c          557 g          816 t
ORIGIN      
        1 aataattgtc cggtattcat ttccggtatt aatactttat caaagcctgc aactaaactg
       61 cctaaatttt tcggaaaagg tttcatatag cgtacatgtg catgcgatac atctaaaccc
      121 tgtgcgcgtg cagctattac agcgctttta atggccccgt aagtactgcc ccatcctaaa
      181 accaatagtt tgccactttc attgccgtta tcaatgtttt gcaaaggaat agagtcggca
      241 accttgttta ctttggcttc gcgcatgcga accataaact catggttatn nnnnnnnnnt
      301 aactaatgtt gccggtttca tgttgtttct ctaagcctcc aatacgatgc tctaagccgg
      361 gtgtaccggg tattgcccaa gggcgcgata atttatcatc acgtttgtaa ggcaaatatt
      421 tttcgcctcc gtttttgtct tttgcaaaat caactttaat ctttggcaaa tcggcttgcg
      481 ttggaaaacg ccgaggctcg gcaccgttgg caatgtaacc gtcacttaaa aacataacgg
      541 gagtcatata ctcaattgca atgcggcagg cttccagcac catggtaaag caatcgctgg
      601 gtgtacaggc ggccactatg ggcatgggtg cttcgccatt acggccatgt aaggcctgca
      661 ataaatcact ttgttcggtt ttggttggca agcctgttga cgggcccccg cgttgtacgt
      721 ttataattac caaaggcatt tctaacataa cacctaatcc cattgcttcg ccttttaacg
      781 caataccggg acccgatgaa gccgtaactg caagtgcacc accaaatgcc gcgcctatgg
      841 tagcacaaat ggccgctatt tcatcttcgg cctggaaggt gcgcacattc atgtttttgt
      901 acttggccaa ttcatgtaaa atatcagaag caggcgttat ggggtaggtg ccgtaaaaca
      961 tgggcaaacc cgattgctgt gtagccgcta tcaagcctaa ggctgttgcc tgattaccca
     1021 taatgctgcg gtatgtaccg ggtttcatgg gtgcagcacc aatatgataa cgggttatga
     1081 tggtttctgt tgtatcgcca taagcatatc cggcttttaa tatgcggatg ttggcatcta
     1141 atatttcggg ctttttcgaa aatttttcgg taaggaattt tatcgaagcg tctaacgacc
     1201 tgctgtacat ccagtacaaa aagcctaaca caaacatgtt tttgcttcgg tcaatttctt
     1261 tagttcccat gccgctttcg cttaaggcag cacgtgttag tttggtaaca tcaattttta
     1321 ttaagcggaa accatccaaa ctattatcgt caagcggatt tacagcaaca ttagccagac
     1381 gcaagttttt aggatcaaac ccatcaatat tggcaatgat ggtaccgcct tttttaaggc
     1441 ctttaatatt ggcttttaaa gcggctgcat tcatggccac caacacatcg caggtgtcgc
     1501 ccggtgtata aatgtttaca ctgccaaaat gtaactggta cccactaacg cctgccagtg
     1561 tacctattgg ggcgcgtatc tcggcaggga aatcaggaaa cgtacttaaa tcattaccca
     1621 ataaggctgc agttgttgta aattgtgtac cggttaactg cataccatcg cccgagtcgc
     1681 cagcaaattt tataattacc tgttctaaaa cttctgtagt attttccatg atgctaattt
     1741 ttattgaata attaaagttt gcaaaaataa gaggttgcca ttggtttgca agtaagctaa
     1801 atttgaaaca aagcaaaacc tttattaaat ccttgtttta ttttgaacaa atgtttttta
     1861 agcaattaaa catttacagc aaaactgaaa ccagcttgca tttgcatctt ataaaaatgt
     1921 ttgcgtatta acccaattga gcatacacga tttatccatg gtaagaaaag gcgttttata
     1981 catttgcaca ctaatttata catgatgcat aaagccggat ttgtaaacat tataggtaag
     2041 cctaacgtag gcaaaagcac tttgcttaat gtattgctgg gcgaaaatct ggcaattgta
     2101 aaccccaaag cacaaactac ccgtcacaga attaaaggcg ttttaaacgg taccgattat
     2161 cagattgttt tttcggatac gcccggtata atgaaaccgg catacaaact acaccataaa
     2221 atgttagatg ccgtaaacga aacttttacc gatgctgatg ttatgattta cgttgccgaa
     2281 attggcgaaa cagaaatgga tgatggtttg aaggaaaaat taaaaacctt aaaagtgcct
     2341 gtgtttttgg tgctcaacaa aatggatacc atcaatcagg aacagttaga gaatgcattg
     2401 cagcattgga aatcggtttt cgattttaca accattattc caatatcggc attgcataaa
     2461 tttaatacgg catttataat cgaaaatatt ataaaacatt tgccctttgc cgatgcttat
     2521 tatgataaag aaacactaac cgacagaaat gaacgcttct ttgtaactga gatattacgc
     2581 gaaaaaattt taagcctgta tcaaaaagaa ataccctaca gtaccgaagt tgccattgtt
     2641 tattttaaag atgaacctaa tatcagtaaa ataggaacgg taatatatgt ggagcgcgag
     2701 tcgcaaaaag caatagtgtt aggacatcag ggaaaagcca taaaacggtt aggaacggag
     2761 gctcgcaaaa gcatggaaac ttttt
//
LOCUS       DAOMAA010000168         2783 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_30163_length_2783_cov_2.858871, whole genome shotgun sequence.
ACCESSION   DAOMAA010000168 DAOMAA010000000
VERSION     DAOMAA010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30163_length_2783_cov_2.858871"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="PLO59_03135"
     CDS             complement(<1..301)
                     /locus_tag="PLO59_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00124.1"
                     /translation="MKKSILLLCFIWLTTCVKSQDWRNEMLKPNRNFYDVQRKANQFF
                     NLDSKAGQKNMQQLFQTNKPDEHEGEAGYKIYKRWEEFWKTRVYSDGSFPRPDVVY"
     gene            493..1098
                     /locus_tag="PLO59_03140"
     CDS             493..1098
                     /locus_tag="PLO59_03140"
                     /inference="COORDINATES: protein motif:HMM:NF025492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HQV00125.1"
                     /translation="MKKLLALLIFPAYLHAQSDTLKTYATVVDGDTLPMVYMQTVNVV
                     GALSPQAAAQLKAYYILRRDVLRTYPYAKFAAQKVRELNTEASQLKNNRARKKFYKSK
                     EDELAKLFEDDLRKLSVNQGRILMKLIERETGNTTYALVKEMRGGFKATLWQGMARLI
                     GDNMRETYDAGGKDAAIEAIVQQIENGTLPIPAAYQNNKLK"
     gene            complement(1122..1601)
                     /gene="ribH"
                     /locus_tag="PLO59_03145"
     CDS             complement(1122..1601)
                     /gene="ribH"
                     /locus_tag="PLO59_03145"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020532711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HQV00126.1"
                     /translation="MSSSLKNLSQYQKEQLPDVSHRAFGIVKAKWNTEITTALYQACY
                     DTLIEAGVEAGQIYTIEVPGSFELTSGARIMAINHKVDAVICLGCVIQGETRHFDFIC
                     QAVANGLTQLCIQYHKPFVFGVLTPNTQQQAIDRAGGKHGNKGVEAAVTAIEMVALF"
     gene            complement(1669..2343)
                     /locus_tag="PLO59_03150"
     CDS             complement(1669..2343)
                     /locus_tag="PLO59_03150"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQV00127.1"
                     /translation="MAEKKEETINIDETVHKVEDYVQENRKSLLIIAGAAIAVLVGYF
                     AYSNFWVKPAEESAQKEIFAAQQYFKMDSIDLAINGKGKEKGFKQIADQYSSSNTGNL
                     ANYYLGVLYMKKGQFQNAIDALKKYDAEDDITGAMALGLMGDAYMELGNNDEALTYYK
                     KAVGYDNNEFTAPLFMRKSAFTYELQGKWSEALDMYKKIQSDYSASTEGREIGKYIAR
                     AQAMIK"
     gene            2568..>2783
                     /locus_tag="PLO59_03155"
     CDS             2568..>2783
                     /locus_tag="PLO59_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008509366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQV00128.1"
                     /translation="MLYLAHLSLLHFKNYDHLQINLHSGVNVFTGPNGLGKTNLMDAI
                     HYLCMCKSFLSTDSANIKRGAPAAMIQG"
BASE COUNT          850 a          543 c          463 g          927 t
ORIGIN      
        1 tataaactac atcgggtctg ggaaatgaac catcggaata aacgcgtgtt ttccaaaact
       61 cttcccatcg tttgtatatt ttataacctg cttcgccttc atgttcatca ggtttattgg
      121 tttgaaacag ctgttgcata tttttttgtc ctgcttttga atcaagattg aagaattgat
      181 ttgctttccg ttgcacatca taaaaattac gatttggttt tagcatttca ttacgccaat
      241 cttgtgattt tacacaagtg gttaaccaaa taaaacataa caggaggatt gattttttca
      301 tctttaaata acttattagt tactgcaaaa taaacattat gcagcgtaat ttctatagtc
      361 aatatatcta tcactgcccg acctgttttg taatacaact tacttaaatt ttgaatgaag
      421 taggcaatta catttaattg tattggctac tttgcacaac gtttgattaa agcaataacc
      481 aaatattata aaatgaaaaa gctattggct ttactaattt tcccggctta cctgcatgca
      541 caatctgata cattaaaaac ttatgcaaca gttgttgatg gcgatacctt gcccatggtt
      601 tacatgcaaa cagttaatgt agttggtgcg cttagcccgc aagctgcggc acaacttaaa
      661 gcttattaca tattaaggcg cgatgtattg cgcacttatc cgtatgcaaa gtttgctgct
      721 caaaaagtta gggagttaaa tactgaagcg agtcaattaa aaaacaatcg cgcacgtaaa
      781 aaattttaca agtcgaaaga agatgaatta gccaagcttt ttgaagatga tttacgaaag
      841 ctaagcgtta accaaggacg cattttaatg aaattgattg agcgcgaaac cggcaataca
      901 acttatgcct tagttaaaga aatgcgtggc ggttttaaag ctacactttg gcaaggtatg
      961 gcacgactta ttggcgataa catgcgcgaa acttatgatg caggtggtaa agatgctgct
     1021 attgaagcca ttgttcagca aattgagaac ggaaccttac ctatacctgc agcctatcaa
     1081 aacaataaat taaagtaacc ttttacggtg cagcgttaac cctaaaagag cgcaaccatt
     1141 tcaatagcag ttacggcagc ctcaacgcct ttattgccat gtttgcctcc tgccctatct
     1201 attgcctgct gttgtgtatt aggtgttaaa acgccaaaaa caaatggctt atgatattgt
     1261 atgcataatt gtgtgagtcc attagccaca gcctggcata taaaatcaaa atggcgcgtt
     1321 tcgccttgta taacgcaacc caaacatatt acggcatcaa ctttatggtt aattgccata
     1381 atacgcgcac cacttgttaa ttcaaaacta ccgggcactt caatggtata tatctgcccg
     1441 gcttcaacac ctgcttcaat taatgtatca taacatgcct gatacagtgc cgttgtaatt
     1501 tctgtgttcc attttgcttt aactatacca aatgcgcgat gacttacatc gggtagttgc
     1561 tctttttggt attgcgataa gttttttaat gatgatgaca taaatattta ttaaaaaaaa
     1621 acagcagtac tatcatactg ctgtttattt aacttttgct tttcgaaatt atttaatcat
     1681 tgcctgtgcg cgtgctatgt actttccaat ttcgcgtcct tcggtgctgg cactgtagtc
     1741 tgattgaatt tttttataca tatctaaagc ttcgctccac ttgccttgca gttcgtatgt
     1801 gaatgcggac ttacgcataa ataacggtgc cgtaaattca ttgttatcgt agcccacagc
     1861 ttttttatag taggttaatg cttcgtcatt atttcctaat tccatgtaag catcgcccat
     1921 taagcctaaa gccatagcac cggtaatatc atcctcggca tcatactttt ttaaagcatc
     1981 aattgcattt tgaaactgac cctttttcat atacaaaaca cccaagtaat aattggccaa
     2041 attgcctgtg tttgatgaac tgtattggtc ggctatctgc ttaaaccctt tttctttgcc
     2101 tttgccatta atcgctaaat caattgaatc cattttaaaa tattgctgtg cagcaaaaat
     2161 ttctttttga gcgctttctt cagcaggttt cacccaaaaa tttgaatacg caaaatatcc
     2221 aaccaataca gcaatagcag caccggcaat aattaataaa cttttgcgat tttcctgaac
     2281 ataatcttca accttatgaa cggtttcatc aatgttaatt gtctcttctt tcttttcggc
     2341 catttttata actatttttt tttaaggtgc gcaaaagtaa aattaattac catatacact
     2401 acaatttaaa ttaagcctgt aattacttgg ttttatggta gttgacagct aaatttttaa
     2461 aaatatcatc ttaaactgta ttgaaacact aaaattgaag cacctttgtt ccgtttccac
     2521 acaatcctgc attataaaaa caaccagtac cctattgata aaatttcatg ttgtatttgg
     2581 cgcatttaag cttgcttcat tttaaaaatt atgaccacct tcagattaac cttcatagtg
     2641 gcgttaatgt ttttaccggg cctaacggat taggcaaaac caatttaatg gatgcaattc
     2701 attacctatg tatgtgtaaa agctttttaa gtactgacag tgccaatatt aaaaggggtg
     2761 ctccggcagc aatgatacaa ggc
//
LOCUS       DAOMAA010000169         2764 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_30566_length_2764_cov_3.110373, whole genome shotgun sequence.
ACCESSION   DAOMAA010000169 DAOMAA010000000
VERSION     DAOMAA010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30566_length_2764_cov_3.110373"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="PLO59_03160"
     CDS             complement(<1..759)
                     /locus_tag="PLO59_03160"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQV00129.1"
                     /translation="MENQLKVLYVDDEENNLISFKSMFRKLYKVYTAISAQEALQIIE
                     NNEMGIIISDQRMPGCTGIQFLAQTIETYPNTIRLLITGQTDIDTVIEAINTGQISKY
                     IQKPWDWDKFTLTLEYCADLYRSRIQLAEKNAQLEKTNDELNRFVYSASHDLRSPLMS
                     VLGIVQYARMEPEMLVAEEYFKVIESSILKLDNYTKRIIEYYQNSRLEEINDVIDFKI
                     LVSDCVESLSNLEPSVVFNVTINQTTNFISDDFRI"
     gene            complement(786..>2764)
                     /locus_tag="PLO59_03165"
     CDS             complement(786..>2764)
                     /locus_tag="PLO59_03165"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HQV00130.1"
                     /translation="QLNNFNRITTDVPNFGISNSVFWLKLKISNQTNVGDFLLQVSHP
                     GLDKISCYQINDDTIFSEAHSGEYNSFNHRVIRDPDYIFNLQLPSHKITTILLQVSAR
                     ENIQIPIFIGTPDRISESNKKRNLAVGIYIGIMLVMFFYNAFLYVTIKDKVYLYYVIY
                     LATVLLTQASIHGYTFMVLWPNIPLLAVYSSFIFPTLVGIASMVFMREFLHVKFFYPS
                     HNKGFIIFMFAYVCASAFSLFGLFKVSFILIELIATLVSLYMLYVGYLIYKKGFRPAG
                     YFLLAWTVFLIGVTVYVLKDFSILPYNNYTLYMMPLGSAAEVVLLSFALADRINILKK
                     EKEESQAETMAALREKEKLISEQNLMLEQKVNERTQELQIANTELGITLKNLKDAQAQ
                     LVNAEKMASLGQLTAGIAHEINNPINFVSANIRPLQLDINDILLIIEKYDALNNAADF
                     DQSKTDIEAYKKEIDFDYLKHEINILINGIEDGAKRTAEIVKGLKNFSRLDESDIKEA
                     NLNEGIESTLVLLRNSMPKSLTLIKNLNVLPNIECYPGKLNQVFMNVLNNALQAIEKN
                     NQQKNHVLTITSILDNDFIKITVADTGVGMDEKVKSKIFEPFFTTKDVGEGTGLGMSI
                     VYSIIESHHGRIEIESAPNQGSEVMLYLPLRLKR"
BASE COUNT          833 a          477 c          476 g          978 t
ORIGIN      
        1 tatgcgaaaa tcgtctgaaa taaaatttgt ggtttgattt atggtaacat taaatacaac
       61 actcggttcc agattgctta atgattctac acagtcgctt actaagattt taaaatcaat
      121 tacatcatta atttcttcca ggcgcgagtt ttgataatat tcaataatgc gtttggtgta
      181 attatcaagt tttaaaatac tgctttcaat aaccttaaag tattcttcgg ctaccaacat
      241 ttcaggctcc attctggcat attgaactat acctaaaact gacattaatg gcgagcgtaa
      301 gtcatgcgat gcactataaa caaacctgtt aagttcatcg tttgttttct cgagctgtgc
      361 atttttttcg gctaattgta tgcgcgatcg gtataaatca gcacaatatt ccagggttaa
      421 tgtaaactta tcccaatccc atggtttttg tatgtattta ctaatttgtc cggtattaat
      481 ggcttcaatt acggtatcaa tatccgtttg gccagttata agcagcctga tagtatttgg
      541 ataggtttcg atagtttgtg ccaaaaactg aataccggta caaccgggca ttcgttggtc
      601 ggaaataata atacccattt cattgttttc aataatctgc aaagcttctt gggcacttat
      661 tgctgtatat actttataca atttgcggaa cattgattta aatgaaatca ggttgttttc
      721 ctcgtcatca acataaagta ctttaagttg gttttccata ctgtacctgt aaattattgc
      781 gtcttttatc tttttaatcg taacggtaaa tataacatta cttcactgcc ttgattgggt
      841 gccgattcaa tttcaatcct tccgtggtgg ctttcgataa ttgaatatac aatagacatt
      901 cccaaaccgg taccttcgcc cacgtctttg gttgtaaaaa agggttcgaa aattttagac
      961 ttcacttttt catccatccc tacaccggtg tctgctacgg taattttaat aaaatcatta
     1021 tctaatatag atgtaatggt gagtacatga tttttttgct gattgttttt ttcgatagct
     1081 tgtagtgcgt tattaagtac attcataaac acctgattaa gtttacccgg gtaacattcg
     1141 atattgggca atacgttcag atttttaata agtgttaggc tttttggcat tgagtttcgt
     1201 aataaaacca gcgttgattc tattccttca ttaaggttcg cttctttaat atcactttca
     1261 tctaaacgag agaaattttt taagcctttt acaatttctg ctgtgcgttt ggctccatct
     1321 tcaatgccat taattaaaat atttatttca tgttttaagt aatcgaaatc aatttctttt
     1381 ttataagctt caatgtcagt tttagattgg tcaaagtcag cggcattatt taatgcatcg
     1441 tatttttcaa ttatcaataa aatatcgttt atatccagtt gtaatggtct gatgttggcg
     1501 cttacaaaat taattgggtt gtttatttca tgcgctatac cagcagttag ttgccctaat
     1561 gatgccattt tttcggcatt aactaattgg gcttgtgcat cttttaaatt ttttaaagta
     1621 atacccagtt ctgtattggc aatttggagt tcttgcgttc gttcgtttac tttttgttca
     1681 agcattagat tctgctcaga aatcagtttt tccttttcgc gtaaagctgc catggtttca
     1741 gcttgcgatt cctctttttc cttttttaaa atattaatcc ggtcggctaa tgcaaatgaa
     1801 agcagcacca cttcggcagc cgaacctaat ggcatcatgt aaagtgtata attattgtaa
     1861 ggtagtatgc taaaatcttt taatacataa accgttacac ctatcaggaa aacagtccat
     1921 gctaataaaa aatagccggc aggtctaaat ccctttttat aaataaggta gccaacataa
     1981 agcatgtaca gcgataccag cgttgcaatt aattcgatta agataaaact aactttaaac
     2041 agaccgaata atgaaaatgc cgatgcgcat acgtatgcaa acatgaaaat tataaaccct
     2101 ttattatggc ttggataaaa gaattttaca tgcaaaaatt cacgcataaa aaccatgctg
     2161 gcaatgccaa ctaaagttgg gaaaatgaat gaactataaa cagccaataa tgggatattg
     2221 ggccatagta ccataaaggt atatccatga atacttgctt gcgtaagcag tacggtggct
     2281 aaataaatta catagtataa atatacttta tcttttattg ttacgtataa gaaggcattg
     2341 tagaaaaaca ttaccagcat tataccgatg tatatgccaa cggctaaatt tctttttttg
     2401 tttgattcgc taatccgatc cggagtacca ataaatatgg gtatttggat gttttcccgg
     2461 gcacttactt gtagcagtat ggtagttatt ttatgcgaag gtaactgcaa gttgaagata
     2521 taatccggat cgcgtattac cctgtggtta aaactattat actcgcctga gtgtgcttca
     2581 gaaaaaatgg tgtcatcatt aatctgatag caactaattt tatctaaacc cggatgtgaa
     2641 acctgtaaca aaaaatcacc tacgttggtt tgattggaaa tttttaactt aagccagaaa
     2701 acagaattgc ttatgccgaa gttgggtaca tcggtggtaa ttctgttaaa gttattaagt
     2761 tgtt
//
LOCUS       DAOMAA010000170         2761 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_30632_length_2761_cov_3.087214, whole genome shotgun sequence.
ACCESSION   DAOMAA010000170 DAOMAA010000000
VERSION     DAOMAA010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30632_length_2761_cov_3.087214"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1185
                     /locus_tag="PLO59_03170"
     CDS             <1..1185
                     /locus_tag="PLO59_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010519919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HQV00131.1"
                     /translation="NTQPAWTKKPVDLTADDYKNFYRELYPLTFDEPLFHIHLNVDYP
                     FNLTGILYFPKLKKSFEPQKNKIQLYSNQVFITDAVEGIVPEFLTLLHGVIDSPDIPL
                     NVSRSYLQSDGNVKKIASHITKKVGDKLHELFFDNRADFETKWDDIGLFVKYGMLSDE
                     KFNERAKGFCLLKNEAGKYFTLEEYQNLVKPNQTNTDGKVVYLYTNNTTEHHSLIAQA
                     QKRGYDVLVLDSPLDAHFINMLESKNEASFARLDADTLDNLIKKSAETTSALTDENKQ
                     QLQPVIESVLNDSSYKVQFENMQADDAPVLITQSEFARRMKEMSAVGGGYDFMGTMPE
                     MYQVTVNTNHPVIMKIASQDDVEKQKTDVRHTIDLALLAKGLLKGQALTEFINRSYTQ
                     IN"
     gene            1252..1770
                     /locus_tag="PLO59_03175"
     CDS             1252..1770
                     /locus_tag="PLO59_03175"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020532376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HQV00132.1"
                     /translation="MPQKKVKAIDTLCIATELVLPNDTNTLGNLMGGRLLHWMDIAAA
                     VSAHRHSHRIVVTASVNNVSFNQPIKFGDIVTISAKVSRAFNSSMEVFMDVWVENNAE
                     GKKVKCNEAIYTFVAVDQLGNPINVPELIPESEEEKQRFAGALRRKQLALILAGKMKP
                     SEATELKALFDI"
     gene            complement(2101..>2761)
                     /locus_tag="PLO59_03180"
     CDS             complement(2101..>2761)
                     /locus_tag="PLO59_03180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HQV00133.1"
                     /translation="LLVNFKINKMPNLHIVDRYLNTVKTLGIKNDNEGLDYFLPPALS
                     NADSLTQIPYQSFKYIAVAIGAQHQTKRLPVAKLISICNKLPLPVVLLGSEADNAQAE
                     IIAHEVPQCLNACGKLSLHQSAWAVKHAAFVLTHDTGLMHIAAAFNKRIISVWGSTVP
                     AFGMTPYLGYNRMQGSAYIAEVAHLNCRPCSKIGYEKCPKKHFNCMQQIDESAVIAAF
                     R"
BASE COUNT          916 a          503 c          516 g          826 t
ORIGIN      
        1 aatacacagc cagcctggac taaaaaaccg gttgatttaa cggccgatga ttacaaaaat
       61 ttttatcgcg aattgtaccc actcaccttt gatgagccat tattccatat acacttaaat
      121 gttgattatc catttaacct gaccggaatt ttatacttcc ccaagcttaa gaaatcgttc
      181 gagccgcaaa aaaataaaat acagttatac agtaatcagg tgtttattac cgatgccgta
      241 gaaggtattg tgcccgagtt tttaacctta ctgcatgggg ttattgactc gcctgatatt
      301 ccgcttaatg tgtcgcgtag ctatttacag agcgatggaa atgtgaaaaa aatagcttca
      361 cacattacta aaaaagtagg cgataaatta catgaattgt tttttgacaa tcgtgccgat
      421 tttgaaacca aatgggacga tattggtttg tttgtgaaat acggtatgtt aagcgatgaa
      481 aaatttaatg agcgcgccaa aggtttttgt ttgcttaaaa acgaagccgg caaatacttt
      541 acgcttgaag aatatcaaaa cttagtaaaa cccaaccaaa ccaataccga tggtaaggta
      601 gtttacttat acaccaacaa tacaacagag caccacagct taattgccca ggcacaaaaa
      661 cgcggttatg atgttttggt gcttgatagc ccattggatg cacattttat aaacatgctc
      721 gaaagcaaaa acgaagcatc gtttgcacgt ttagatgccg atactttaga taatttgata
      781 aaaaaatcgg ccgaaaccac atcagcatta accgatgaaa acaagcaaca attacaaccg
      841 gttattgaaa gcgttttaaa cgatagcagt tataaagttc agtttgaaaa catgcaggct
      901 gatgatgcac ctgtacttat tacacaaagc gaatttgcaa gaagaatgaa ggaaatgagt
      961 gcagttggtg ggggatatga ttttatgggt accatgcctg aaatgtatca ggtaacggta
     1021 aacaccaatc atccggttat aatgaaaatt gcctctcaag atgatgtaga aaaacaaaaa
     1081 acagatgtaa gacacaccat tgatttagct ttgttggcaa aaggcttgct aaagggccag
     1141 gcacttaccg agtttataaa ccgtagttat acgcagatta actaattaca attaacagcc
     1201 atgccacctc atggctgttt attgtttcaa taaatttcaa ttaacaaaac aatgccgcaa
     1261 aaaaaagtaa aagcaattga tacactatgt attgccactg aactggtatt accaaatgat
     1321 accaatacat taggtaattt aatgggtggt cgtttattac attggatgga tatagcagca
     1381 gccgttagtg cgcacaggca tagtcatcgt attgtagtta cggcatcggt aaacaatgta
     1441 tcatttaatc aacctattaa gtttggcgat attgtaacca tatcagcaaa agttagccgt
     1501 gcatttaact cgtcaatgga ggtatttatg gatgtatggg ttgagaataa tgctgagggt
     1561 aaaaaagtaa aatgcaacga agctatttac acctttgtag cagttgatca gttaggtaac
     1621 cccattaatg taccagaatt aatacccgaa agcgaagaag agaaacaacg ttttgcaggt
     1681 gcattacgca gaaaacaact ggcattgata cttgccggaa aaatgaaacc aagcgaagct
     1741 acagagctta aagctttgtt tgatatttag attaaacaca aattaaattt tgtaaaatat
     1801 tgcatatact ccaactgtta catttggttt ttggctgcta tgttgcatta tattttaacc
     1861 atgcagccac ctggccttat ataccttttg cgctattttt agcgctgggt gtttccgaaa
     1921 aagtaatact aacaaaactc aaaccgtttc attggatttt tactgctata catgcaattg
     1981 tactttttgt aatcaattta caaattccat tgcccttcgt tatttatgca ataataaata
     2041 tggctttatt tttagctgtt gccatcaata atgcttttcg ggaacagcaa gcaagagttg
     2101 ttatctgaag gcagctatta cggcactttc atctatttgt tgcatacaat taaaatgctt
     2161 tttggggcat ttttcataac caattttact gcaaggccta caatttaaat gggcaacttc
     2221 ggctatgtag gcactgcctt gcatccggtt ataacccaaa tacggagtca tgccaaatgc
     2281 aggcactgta ctaccccaaa cagatataat acgtttatta aatgcagcag caatatgcat
     2341 taaaccggtg tcgtgcgtta atacaaaagc agcatgtttt accgcccatg ccgattggtg
     2401 taaacttagt ttaccacatg catttaaaca ctgtggcacc tcatgtgcaa ttatttcggc
     2461 ctgtgcatta tcagcttcac tgcctaatag tacaacgggc aaaggcaact tattacaaat
     2521 agaaataagt tttgcaaccg gcaagcgttt ggtttgatgt tgtgcaccaa tggcaactgc
     2581 aatatattta aagctttgat acgggatttg tgttaaacta tcggcattac ttaaggctgg
     2641 gggtaaaaaa taatctaatc cttcattatc gttttttata cctaatgttt tcacggtgtt
     2701 caaatacctg tcaacaatat ggagattggg cattttgtta attttaaaat taaccaataa
     2761 c
//
LOCUS       DAOMAA010000171         2761 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_30634_length_2761_cov_2.964893, whole genome shotgun sequence.
ACCESSION   DAOMAA010000171 DAOMAA010000000
VERSION     DAOMAA010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30634_length_2761_cov_2.964893"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2761)
                     /locus_tag="PLO59_03185"
     CDS             complement(<1..>2761)
                     /locus_tag="PLO59_03185"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HQV00134.1"
                     /translation="YTLSITDATGCVRVTEKTITQPEQIVITCSPVDVTTACSNDGHI
                     FTQVTGGSNPFDYLWNDGATVANRHNLATGSYTVTVTDKKGCSLIQTVMVGGQAPVTV
                     NGTVQQVSCSNPFGGSIQLAVNGGTAPYDYMWNTGATTDALNNVGVGSYTVTIADENN
                     CTVSATFILNAPPAPTAITSGSNITCFGLTDGNATVIASGGTSPYTYLWNNGSTNTNI
                     VGLSAGSYTATVTDALGCTAISSVTIIEPAKLIPLRNKTNVSCFGGNDGSVVWQTYGG
                     TPLYGYLWSDGSTLSNRFNLTALLYTITIYDANGCTDTVSIKINQPLNAVTAQLNVTN
                     QSSYCVNDGSIEAIGAGGVPPYSYLWSNGATTATATGLSATLYTVTVSDFNGCTYQGS
                     ANVSSPNGMSVSASITGTCTGASTGAIAITVNSGMPPYTFLWNDGSTLQNRSNLAATT
                     YIVTVTDALSCTTGGSFVVTALPEPQITWAENHVTCNGLNNGTIDITEGVAAPYQYLW
                     NNGATSQDVNNLIPSNYTVSVTNVLGCTAMFGFTISEPAVLDGNKTKQSVSCNGGKDG
                     SISFNTTGGTAPYTYQWSNGYSTSSANNLEYGNYTVTITDANGCTISYSTKINEPTAI
                     SISTISTDCSSYGNNDGTATVTINGGGVGPYTYIWSNGQTNAQANKLISGTYTVTVTD
                     ANGCTKTANAFVNEPAPVCLGFVTGHVITYYNPPSGSDTLANYLDNNFGSVFNDSLVL
                     GCNTGYTLSFTTAQAVKDFLPSNGNPKDLDQSFVNPDTVSLQNTLAAQLVALALNIRF
                     DLTNPDFASMSTVNFRDLNIVEPGPFYGYTVQQLYDEANSFLGGCGSLNTGAQIRTMV
                     SVVNSSWNLGTQTNTALACPGAPSAKFEFGKSASDELNLNAYPNPNRGNFNLSYYAVN
                     TEPVT"
BASE COUNT          816 a          576 c          527 g          842 t
ORIGIN      
        1 atggtaaccg gctcggtatt tactgcatag tagctcaaat taaaattgcc tctgtttgga
       61 ttgggatatg catttaagtt tagttcatcg cttgctgatt ttccaaactc aaactttgca
      121 cttggcgcac ccggacaagc caaagctgta ttggtttgtg tacccaaatt ccaagatgag
      181 tttactacac taaccatggt acgaatttgt gcacctgtgt ttaagctgcc acaacctccc
      241 agaaaactgt ttgcttcatc atacaattgt tgtactgtat aaccataaaa tggcccgggt
      301 tccacaatat ttaaatcgcg gaaattaacc gtgctcattg aagcgaaatc ggggttggtt
      361 aaatcgaaac ggatgtttaa tgccaatgct actagttgtg cagctaaggt gttttgaaga
      421 cttaccgtat cggggtttac aaatgactga tctaaatctt ttggattacc gttagaaggt
      481 aaaaagtctt taacagcttg tgctgtggta aatgataatg tgtaacctgt gttgcaacca
      541 agcactaacg aatcgttaaa tacacttccg aaattattat ccaaataatt ggctaaagtg
      601 tcgcttccac tgggcgggtt atagtaggta ataacatggc ccgtaacaaa acccaaacaa
      661 acaggtgccg gttcatttac aaatgcattt gctgttttag tacaaccgtt tgcgtcagta
      721 acagttactg tataggtgcc tgatatcagt ttattggctt gtgcattggt ttgcccatta
      781 ctccatatat aagtataagg ccctacacca ccaccattta tggtaactgt agctgtacca
      841 tcattattgc catacgatga gcaatcagta ctaattgttg aaatacttat tgcagttggc
      901 tcattaattt tggtgctgta tgaaatagta catccattag cgtcagtaat tgttaccgtg
      961 taattgccat actctaaatt attagctgat gaggtggagt acccgttact ccattggtaa
     1021 gtatatggtg ccgtaccacc tgttgtgtta aacgaaatac taccgtcttt accaccatta
     1081 caactcacac tttgtttggt tttatttcca tctaaaacag ccggttcgga aattgtaaaa
     1141 ccaaacatag ctgtacatcc caatacattg gttacagata ccgtatagtt tgaaggtatc
     1201 agattattta catcctgact tgttgcacca ttattccata aatattgata cggtgctgct
     1261 acaccttcag taatatcaat agttccattg ttaaggccat tgcaagtaac atggttttcg
     1321 gcccaggtaa tttgtggttc gggcaatgcc gtaactacaa acgatccacc tgttgtacat
     1381 gacaatgcat ctgttacagt tactatatag gttgtagcag ctaaatttga acggttctga
     1441 agcgtactgc catcattcca taaaaaggta taaggcggca tacctgaatt aactgtaatt
     1501 gctatggcac cggttgatgc acccgtacag gttcctgtta tacttgccga aacactcata
     1561 ccattagggc tactaacatt agccgaaccc tgataggtac atccattaaa atcgctaacg
     1621 gttacggtat ataaagtggc acttaaaccc gttgccgtag cggtagtagc accattactc
     1681 cataaataac tgtatggcgg aacaccaccg gcaccaattg cttcaatgct tccatcattt
     1741 acacaatatg atgattgatt tgtaacattc agttgtgcag ttacagcatt taatggttga
     1801 ttaattttaa ttgaaaccgt atcggtacaa ccatttgcat cataaatagt gatggtgtat
     1861 agtagtgccg ttaagttaaa tctatttgat agtgttgaac catcgctcca cagataacca
     1921 tacaatggtg taccaccata cgtttgccat acaacagagc catcgttgcc accaaaacat
     1981 gatacattgg ttttattacg taacggaatc agtttagcag gttcaataat agttacagaa
     2041 cttattgccg tgcaacccaa tgcatcagta actgttgccg tataactacc tgcacttaaa
     2101 ccgacaatgt ttgtattggt actaccgttg ttccataagt aagtataagg agatgtaccc
     2161 cctgacgcaa tgacggtagc attaccatct gttaaaccaa agcaagtaat attgctgcct
     2221 gaagtaatag cagtaggtgc agggggcgca tttaatataa atgttgccga tacagtacaa
     2281 ttattttcat cggcaatggt tactgtataa gaacccacgc caacattgtt taacgcatcg
     2341 gtagttgcac ctgtattcca catataatca taaggcgctg tgcctccgtt tacagccaat
     2401 tgaatactgc ctccaaacgg attgctacaa cttacttgtt gcacggtacc attaacagta
     2461 accggtgcct ggccaccaac cattaccgtt tgaattaagc tacatccttt tttatctgta
     2521 acagttactg tataggaacc tgttgctaaa ttatgacgat tagctacggt agcaccatca
     2581 ttccataaat aatcaaatgg attggaaccc ccggtaactt gcgtaaaaat atgcccatca
     2641 ttactacaag ctgtagttac atcaaccggt gaacaagtaa taactatctg ttcaggttgg
     2701 gtaattgttt tttcggttac cctaacacaa cccgtagcat cggttataga aagtgtgtag
     2761 g
//
LOCUS       DAOMAA010000172         2752 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_30825_length_2752_cov_2.508713, whole genome shotgun sequence.
ACCESSION   DAOMAA010000172 DAOMAA010000000
VERSION     DAOMAA010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30825_length_2752_cov_2.508713"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1896)
                     /locus_tag="PLO59_03190"
     CDS             complement(<1..1896)
                     /locus_tag="PLO59_03190"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010601516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HQV00135.1"
                     /translation="MNNFETLGLHEFIVRAILELGFETPTPIQQQSIPQLLKERKDLI
                     ALAQTGTGKTAAFGLPLLHLIDANARHTQALILAPTRELCVQICNDLVKFSKYMGHLN
                     IVPVYGGASINDQIRAIKRGAQIIVATPGRLNDMINRNVVDLSHIQFSVLDEADEMLN
                     MGFKEEIDSILDNTPETKNTWLFSATMPKEVREIARNYMFEPIELTVGKKNQGADNIE
                     HIYYVVNARDRYAALKRLVDSHPDIFGIVFCRTKIETQEISEKLIKDGYNADSLHGDL
                     SQQQRDKVMGRYRDKSLQILVATDVAARGIDVSDVTHVINYQLPDEIENYTHRSGRTA
                     RAGKSGQSIVIISPRDVSKIRQIEKVINKQFTQGKVPSGVVVVENQLMGLIKKLHKVE
                     VNDAAIAKYLPGVNDELAELSKEELIKRIVSIEFNRFLEYYRNAEDLNIDGNSRRSEG
                     KKLFLNLGSVDGFDRAQMLSYLCTVTELPKDTFGHIKIKGVYSFIDMTENMIPAALDK
                     FNGEVFKGRKVRADISTNQGGNRSGGSSSRSGSRSGGNSYEDRGRNRSFGGGRSAYNR
                     SDRGGDRGFYNDRNSDKGYDRSRSDRYASDKPKRNNRTESGNTEINKPPYQDFFKSEF
                     GDGKGFDK"
     gene            2288..>2752
                     /locus_tag="PLO59_03195"
     CDS             2288..>2752
                     /locus_tag="PLO59_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00136.1"
                     /translation="MIIKKINSLLSACFILASTGCINSQSQVPVKKSAIIEPTMLKRV
                     VVNYNDTVIMHFVNNAALFNEGWDTLGQAKFWKQIIHLNPDSSIINVAQTRQTIETIS
                     AQKWMQQTEDEKTLAKAWVCQMNNLDSGTCLYVSIGRKDFFEHRKSLATIDKA"
BASE COUNT          821 a          584 c          467 g          880 t
ORIGIN      
        1 tttatcgaag ccttttccat caccgaactc cgacttaaaa aaatcctgat aaggtggttt
       61 attaatttcg gtattacccg attcggtacg gttgtttcgc tttggcttat cgcttgcata
      121 acgatcgctt ctgcttctgt cataaccttt atcgctgttt ctgtcattat aaaaacctct
      181 atcaccgcct ctatcgctac ggttgtatgc actacggcct ccgccaaaac tacggtttcg
      241 gccacggtct tcataagagt tgccaccact gcggctaccg ctgcggctac tacttcctcc
      301 acttcgatta ccaccctggt tagtacttat atcggcacgt actttacgac ctttaaacac
      361 ctcgccatta aatttatcta atgcagcggg tatcatattc tctgtcatat caataaagct
      421 ataaacacct ttaatcttaa tatgaccaaa agtatcttta ggcaattcag ttactgtgca
      481 taaataactc agcatttgag ccctgtcaaa accatcaacc gaacctaaat taagaaacag
      541 ttttttccct tcgcttctgc ggctgttacc gtctatattc aaatcttcgg cattgcggta
      601 gtactctaaa aaccggttaa actctatact aactatacgt tttattaatt cttctttgct
      661 tagttcggcc aactcatcat ttacaccagg taaatacttt gctatcgcag catcatttac
      721 ttcaacctta tgtaattttt ttatcaagcc cattaattgg ttttctacca caactacacc
      781 actgggcact ttaccctgcg taaattgttt attgattact ttttcaattt ggcgaatttt
      841 agaaacatca cgtgggctga taattacaat agattgacct gatttgcctg cacgtgcggt
      901 gcggccacta cggtgcgtat agttttctat ctcatccggc aattgataat taataacatg
      961 tgttacatcg ctaacatcaa ttccacgcgc agcaacatcg gttgccacta aaatttgtaa
     1021 tgatttatcg cgatagcgcc ccattacttt atcgcgttgt tgttgactta aatcgccatg
     1081 taacgaatcg gcattgtagc catctttaat aagtttttcg ctgatttcct gtgtttcaat
     1141 ttttgtacgg caaaatacaa taccaaaaat atcggggtgc gaatcaacca aacgcttcaa
     1201 agctgcatac ctgtcgcggg cgttaaccac ataataaata tgttcaatat tatcggcccc
     1261 ctgattcttt ttacctacgg taagttcaat aggttcgaac atatagttgc gtgcaatttc
     1321 acgtacttcc ttaggcatgg tagcactaaa caaccaggtg ttttttgttt cgggtgtatt
     1381 gtctaaaatg ctgtctatct cttctttaaa acccatgttc agcatttcgt ctgcttcatc
     1441 taacacacta aattgaatgt gactcaaatc aaccacatta cggttaatca tatcatttaa
     1501 ccgccccggt gtagctacaa taatttgcgc accgcgttta attgcgcgta tttggtcatt
     1561 gatacttgcg ccaccataaa ccggcacaat gtttaagtgc cccatgtact tgctaaactt
     1621 aactaagtcg ttacaaatct gtacgcataa ctcacgcgtt ggtgccaata tgagtgcttg
     1681 tgtatgacgt gcatttgcat caatcaaatg caataaaggc aaaccaaatg cagcagtttt
     1741 accggtacct gtttgagcaa gtgcaattaa atctttgcgc tcctttagta attgcggtat
     1801 tgattgttgt tggataggcg ttggtgtttc aaagcctaat tcgaggattg cacgtacaat
     1861 aaactcgtgc aaaccgaggg tttcaaaatt gttcatcatg tttaaattgt gtcaacctta
     1921 taacggtgat caacttaacc tcattgacaa acacccatca taggataact attttttttg
     1981 cgcggcaaat gtatgtttat atcggttaca aaaaaccaat tttggttttt agaaatttac
     2041 aatgcctcaa gaccgttgga aacactgtaa tttaaaccgg tttataacca tactgtaaca
     2101 tatttacaaa tgattttttt cagattacaa attgtaaact gcaaaaattt caaccggctt
     2161 ttaccacaac ataccggttg aaaattaaaa agcttttaca gcgttaaact tatacaatgt
     2221 ttaatattgc aacatcccat aagcaaaaca gactgaaaaa acattatata aagccagaag
     2281 cataatcatg attattaaaa aaataaattc tctgttaagt gcatgtttca ttttagcaag
     2341 caccggttgt attaatagcc aaagtcaggt gccggttaaa aaatcagcca taatagagcc
     2401 aactatgttg aaacgtgttg ttgtaaatta taacgataca gttatcatgc attttgtaaa
     2461 taatgcagcc ttgtttaatg agggatggga caccttgggt caggctaaat tttggaaaca
     2521 gattatacac ctcaatcccg attcgagcat tattaatgtt gctcaaacgc gtcaaacaat
     2581 agaaaccata agtgcacaaa aatggatgca acaaaccgaa gatgaaaaaa cacttgcaaa
     2641 agcatgggtt tgccagatga ataatttgga ttcggggaca tgcttatatg tgagtattgg
     2701 gcggaaagat ttttttgagc atcgtaaatc gttagcaacc attgataaag ct
//
LOCUS       DAOMAA010000173         2750 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_30870_length_2750_cov_2.587013, whole genome shotgun sequence.
ACCESSION   DAOMAA010000173 DAOMAA010000000
VERSION     DAOMAA010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30870_length_2750_cov_2.587013"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..1012
                     /locus_tag="PLO59_03200"
     CDS             26..1012
                     /locus_tag="PLO59_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00137.1"
                     /translation="MIKLLKNNVVLAFIATIGLTGVLFYIRYINGFNIETNGGMPLYN
                     ALLQILPVHFSNVYVCITYFLIIYQALFLNYILVSNEVLPKNTWLFAPVYALLLCFIP
                     QFVTFNPVLVSGSFIIWAFDKILKSYKSNHTLALDFDIGLIIGVASLFYLPMVLKSIL
                     FAIGILILKPFSWRDWVVFLLGLLLPFFLLFVYFFLTNNESLIIIKGNEFKPAINWQQ
                     VIPTNFGISGGFLAILLVLSAFMVVNQFNKSVIKIRSTRQVTLATGIVAILVLLVNTP
                     QTLYPFTLFCPAFSFLITFYLINIKRYWIGECIFWMLIAAVFFNHLIIATAR"
     gene            1085..1789
                     /gene="purQ"
                     /locus_tag="PLO59_03205"
     CDS             1085..1789
                     /gene="purQ"
                     /locus_tag="PLO59_03205"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012930791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HQV00138.1"
                     /translation="MKFGVVVFPGSNCDQDMVDVLESLKVKHQVNVVKLWHKEHDLQN
                     CDMVILPGGFSYGDYLRSGAIARFSPIMQEVIAFAGKGGLVMGVCNGFQILCEAGLLP
                     GALLHNNSRKFICKNVYIKPVTDETYITSKLNTNKALKIPIAHGEGKFYAPDSEMKAI
                     YDNGQILFKYCDALGNVTAESNPNGSIDNIAGITNKTKNVFGMMPHPERACDPYLANT
                     DGRGIFDSILNSMALF"
     gene            complement(1805..2113)
                     /locus_tag="PLO59_03210"
     CDS             complement(1805..2113)
                     /locus_tag="PLO59_03210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HQV00139.1"
                     /translation="MLDMIQKLGELKQKMEESKQRLEAVTVSGSAGDGAVTVTLTGNK
                     KFKSIFIEDAMCLPERKEELTDYIEIAFAKALDAAQDISDKEMMAQGQGLLPNLPGLF
                     "
     gene            2215..2601
                     /locus_tag="PLO59_03215"
     CDS             2215..2601
                     /locus_tag="PLO59_03215"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HQV00140.1"
                     /translation="MSTTATRYTFKKTERLCSRKQIEQLIQTKQHKFSHPLKLMYALN
                     QQNDAKERAQVAFVVPKRLFKKANQRNYIKRLMREAYRINKPEFYKQLEQKQNAYNLM
                     FVYAVADSPNFNDIKKKIVLLLQQLP"
BASE COUNT          853 a          480 c          486 g          931 t
ORIGIN      
        1 caatttgtag tatagataag ccggtatgat taaattatta aaaaacaatg tggtactggc
       61 ttttattgca accattgggc taaccggtgt acttttttat attcgttaca taaacggttt
      121 taatatagaa accaatggag gcatgccact ttacaatgca ttgctacaaa ttttaccggt
      181 acatttttca aatgtatatg tatgtattac atacttttta ataatatatc aggctttatt
      241 tctcaactat attttagtaa gtaatgaagt attgcctaaa aacacatggt tgtttgcacc
      301 tgtttatgca ttgcttttgt gttttattcc acagtttgta acttttaacc cggttttggt
      361 ttcaggttca tttataattt gggcatttga taaaattctg aaatcgtata agagcaatca
      421 tactttagca ttagatttcg atattggttt aataataggt gttgcatcgt tgttttacct
      481 gccaatggtt ttaaagagta tattgtttgc aataggcatt ttaattttaa aacccttttc
      541 ctggcgcgat tgggtagttt ttttattggg attactactt ccgttttttc tgctgtttgt
      601 ttattttttt cttacaaata atgagtcgct tataatcata aaaggcaatg aatttaagcc
      661 tgcaattaac tggcagcaag taataccaac taactttggt ataagcggag gatttttagc
      721 aatactgtta gtattgtcgg cctttatggt agttaaccaa tttaataaaa gtgtaataaa
      781 aatacgcagc acacgtcagg taactttggc aacaggtata gttgcaatac tggttttatt
      841 agtcaataca ccacaaaccc tgtatccgtt tacattattc tgtccggctt tttcattttt
      901 aataaccttt tatcttataa atatcaaacg ctattggatt ggtgaatgca ttttttggat
      961 gctcattgcg gctgtgtttt ttaaccactt aataatagct acagcgaggt aacaatattg
     1021 ttttctgctt ttttagggtg taaaaagcgt tacttttgtt ccgctatttt taaatataat
     1081 tcaaatgaaa tttggtgtag tagtatttcc gggctccaat tgcgatcagg atatggtaga
     1141 tgttttagaa tcgcttaaag taaaacatca ggtaaatgtt gttaagctgt ggcataaaga
     1201 acacgatctt caaaactgtg atatggttat tttaccgggc ggatttagct atggcgatta
     1261 tttacgttca ggtgccatag cacgtttttc gcctatcatg caagaagtaa tagcttttgc
     1321 cggtaaaggt gggttggtaa tgggtgtatg taatggtttt caaatattat gcgaagcagg
     1381 tttgctgccg ggtgcgctct tgcacaataa ctcacgcaag tttatttgca aaaatgtata
     1441 tattaaacct gtaacagacg aaacatatat tacttcaaaa ttaaatacca ataaagcgct
     1501 taaaattccg atagcacatg gcgagggtaa gttttatgcc cccgatagcg aaatgaaagc
     1561 tatttatgat aacggtcaga tactattcaa atattgtgat gcgcttggta atgtaacagc
     1621 cgagtcgaac cccaatggat ccattgataa cattgccgga attactaata aaactaaaaa
     1681 tgtatttggt atgatgccgc atcccgaaag ggcctgcgac ccttatctgg caaataccga
     1741 tggccggggc atatttgata gcattttaaa ttcgatggcc ttgttttagt aattggccgc
     1801 gctattaaaa caagcccggt aaattgggta acaaaccttg tccctgtgcc atcatttcct
     1861 tgtcagaaat atcttgtgca gcatctaatg ctttggcaaa agctatttca atataatcgg
     1921 taagttcttc tttacgctca ggcaaacaca tggcatcttc aataaaaatg cttttaaatt
     1981 ttttatttcc ggtaagggtt actgtaaccg ctccatcacc ggcactgcca cttacagtaa
     2041 cggcctctaa acgctgcttg ctctcttcca ttttttgttt gagctcgccc aatttttgaa
     2101 tcatatctaa catgttatcg cttttaatcg ttaagtaatt tagtttaagt caaagtaaag
     2161 cagctttact gataaaaaac aagttatttg cttttaagat ataagtttta cacgatgtcc
     2221 actactgcta cacgttatac atttaaaaaa accgagcgtt tgtgcagccg taaacaaatt
     2281 gaacagctta tacaaaccaa gcagcataag tttagccacc cgcttaaatt gatgtatgca
     2341 ttaaaccagc aaaatgatgc aaaagagcga gcacaagtgg cctttgttgt acccaagcgt
     2401 ttatttaaaa aagcaaatca gcgcaactat ataaaacgac ttatgcgcga agcctacaga
     2461 attaataagc ctgagtttta taaacaattg gagcaaaagc aaaatgcata taacctgatg
     2521 tttgtttatg ctgttgccga ttcacctaac tttaacgaca tcaaaaaaaa aatagtacta
     2581 cttttacaac aactacccta aagcattgta tttattagct tacaaaccat tcaacgctaa
     2641 gcccaaacac atagaaagta cttctttaca ccatgaaaaa aattttggca acacttttta
     2701 tagggtgcat aacgttttac aaaaatgcaa tttcgccaca tattggcagg
//
LOCUS       DAOMAA010000174         2740 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_31078_length_2740_cov_2.968343, whole genome shotgun sequence.
ACCESSION   DAOMAA010000174 DAOMAA010000000
VERSION     DAOMAA010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31078_length_2740_cov_2.968343"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1422
                     /locus_tag="PLO59_03220"
     CDS             <1..1422
                     /locus_tag="PLO59_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HQV00141.1"
                     /translation="SDLAAKFPKENLPSLGLNSKLIKTEKGIFEKTYKVDGMYGKALK
                     EMVYWLKLAVTVAENPIQAQTLNKLITYFETGDLAAFDTYNISWVKDTTSRIDLVLGF
                     IEVYHDAIGKKGSYEAVISMKDLEASKTIATIAANAQWFEDNSPIATAHKKSKVTGIS
                     AKVITVINEGGDAAPATPIGINLPNAEWIREGHGSKSVSLGNIVASNNFAKAKSPMID
                     EFGSNGQVTALIKKHGALAGMLHTDMHEVIGHASGQLNPGVATTDVTLVNYASTLEEA
                     RADLVALYYIMDQKLVDIGVCPTTDVGKAQYDSYILNGLMTQLYRIKPGNNIEEAHMR
                     NRQLICKWVLEKGETENVIEKIVRNNKTYFVISDYQKLRNLFGQLLAEVQRIKSEGDF
                     NAGKNLVEKYGVVVDQELLKEVHQRCEKLNMAPYTGFIQPTLVPTMNAAGDITDVTIN
                     YNNSFISQMLMYGKKYAFLDIEN"
     gene            1472..2512
                     /locus_tag="PLO59_03225"
     CDS             1472..2512
                     /locus_tag="PLO59_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00142.1"
                     /translation="MLRKMIRKIICIGLILITNHIATAQSFTMQHFTLPKSISIRALA
                     VLNDSVCWLGGSNSTFGYTTDAGITWTTDSIATGKLTDFRSIAVINDSTILLANIGSP
                     ARIYRSSDYGKHFTVVYENNHSDVFIDALSFKNNSEGYAVSDPVNQCILILHTIDGGN
                     TWQPVPCNSITTPDAGEAFFAASNSNIIYTDNGVHILSGGNRSRIFSNHGKGFIAYDT
                     PVIRGAPMHGIYTACAQNQNIFIGGGDYNNKSYNHCNKAVSNNGGISFKLVANGKPPG
                     IISCAQYVPHKSKNLLALSLPGIYYSKNNGTSWKLIDAQANYYTCRFSPSGKVCYLAG
                     PKGNIAILWFKQ"
BASE COUNT          932 a          502 c          543 g          763 t
ORIGIN      
        1 tcggatttgg ctgcaaaatt cccaaaagaa aatttaccat cattgggatt aaacagtaaa
       61 ttaatcaaaa ccgaaaaagg cattttcgaa aaaacctata aagttgatgg catgtacggc
      121 aaagccctta aagaaatggt atattggtta aaattagccg ttaccgtagc cgaaaatccc
      181 atacaagctc aaacacttaa taaactaatc acttatttcg aaacaggaga tttagcagct
      241 ttcgatacct ataacatctc ttgggtaaaa gataccacat cgcgcattga tttggtgtta
      301 ggcttcattg aagtatatca tgatgctatt ggtaaaaaag gtagttacga agctgtaata
      361 tccatgaaag atctggaagc atcaaaaacg atagctacca tagctgccaa tgcccaatgg
      421 tttgaagaca attcgcccat tgcaacagcc cataaaaaaa gtaaggttac aggtataagt
      481 gccaaagtta ttaccgttat aaacgaagga ggcgatgcag caccggccac acccattggt
      541 ataaacttac ctaacgcaga gtggattcgc gaaggccatg gaagcaaatc ggtatcattg
      601 ggcaatattg tagcatcaaa caattttgca aaagcaaaat cgcctatgat tgatgaattt
      661 ggcagcaatg ggcaggtaac agcattaatt aaaaaacatg gtgcactggc aggcatgtta
      721 cataccgata tgcacgaggt aattggtcat gcatcgggtc agttaaatcc gggtgtggca
      781 acaaccgatg ttacattggt aaattatgca agtaccttag aagaagcccg tgccgattta
      841 gttgcattat actacatcat ggatcaaaag ttagttgata ttggcgtatg ccctacaact
      901 gatgtaggta aggcgcaata cgactcctat attttaaatg gattgatgac acaattgtat
      961 cgtattaagc cgggcaacaa tattgaagaa gcacacatgc gcaaccgcca attaatctgt
     1021 aagtgggttt tagaaaaagg tgaaaccgaa aatgtaattg aaaaaattgt acgtaacaat
     1081 aaaacctact ttgtaatttc cgattatcaa aagttgcgta atttatttgg tcagttactt
     1141 gccgaagtac aacgcataaa atcagaaggc gattttaatg ccggcaaaaa tttagtcgaa
     1201 aaatatggtg ttgtagttga tcaggaactt ttaaaagaag tgcatcagcg ttgcgaaaaa
     1261 ttaaacatgg caccatacac aggcttcatc caacctactt tggtaccaac tatgaatgcc
     1321 gcaggcgata taacggatgt aaccattaac tataacaaca gctttatttc gcaaatgttg
     1381 atgtatggta aaaagtatgc gtttttagat attgaaaact aacgcttatt gttgtgatgc
     1441 caaaatttta aaccgtattt attattgcat catgttacgt aagatgattc gaaaaataat
     1501 ttgcattggc ttaattttaa taaccaatca tattgcaaca gcgcaaagtt ttacgatgca
     1561 acattttact ttgccaaaaa gcatttctat cagagcgtta gctgtactta acgatagcgt
     1621 atgttggtta ggtggcagta acagtacatt tggttataca actgatgcag gcatcacctg
     1681 gacaaccgat agtattgcca ccggtaaact aaccgacttc agaagcattg cagtgataaa
     1741 tgatagtact attttacttg caaacattgg aagcccggct cgcatttatc gcagcagcga
     1801 ttatggcaaa cactttacag tagtttatga aaacaatcac agcgatgtgt ttattgatgc
     1861 actttcgttt aaaaataata gcgaaggata tgcagtaagc gatcctgtta atcaatgcat
     1921 attaatactg cataccattg acggtggaaa cacatggcaa cctgtaccat gtaattcgat
     1981 aacaacaccc gatgcaggtg aggcattttt tgcagccagc aacagtaaca taatttatac
     2041 cgataacgga gtgcatatac ttagtggcgg caaccgaagt aggattttta gcaaccatgg
     2101 caaaggtttt attgcatacg acacaccggt tatacgtggc gcacccatgc atggcattta
     2161 tacggcatgt gctcaaaatc aaaatatatt tataggtggt ggcgactaca ataataaaag
     2221 ttacaatcac tgcaataagg ctgtaagtaa taatggtggt ataagtttta aattagtggc
     2281 taacggtaaa ccacccggta taatctcttg tgcacaatat gtaccacata aatcaaaaaa
     2341 cttgttggcc ctaagtttac cgggcatata ttacagtaag aataatggaa caagctggaa
     2401 gttgattgat gcacaagcga attattacac ctgtagattt tcgccatcgg gtaaggtttg
     2461 ttatttagcc ggccccaaag gcaacatcgc tatcctttgg tttaaacaat aataaagtag
     2521 cactttgtta catcatcgta acaacaaata ttttctatgt tatatttctg actgcaaaaa
     2581 ttacttttga tgctatgaaa aaatcaattg tactacttct acttacatgc acaataatta
     2641 atgcatatgc acaacccgat tatgcgggca aagtaaatcc atttgtgggc acaggtgggc
     2701 atgggcacac gtatccggga gccagtgcgc cttttggcat
//
LOCUS       DAOMAA010000175         2740 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_31084_length_2740_cov_2.635754, whole genome shotgun sequence.
ACCESSION   DAOMAA010000175 DAOMAA010000000
VERSION     DAOMAA010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31084_length_2740_cov_2.635754"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1223
                     /locus_tag="PLO59_03230"
     CDS             <1..1223
                     /locus_tag="PLO59_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011584697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQV00143.1"
                     /translation="KKNRGPLGTHAKESHGYFTFPKLEGDISNKMIFRGKEKLVWSLN
                     NYLGLANHPEVRKADADSSSNYGMALPMGARMMSGNSNYHEKLENEIAQFMQKEDCLL
                     CNFSYQAMVSSIDALVDRHDVIVYDSESHACIIDGLRLHMGKRFVYPHNNIENLEKQL
                     ERATKLVNESGGCILVITEGVFGMSGDQGKLKEIVDLKSKFNFRLFVDDAHGFGTMGA
                     TGAGTGEAQGVQSGIDIYFGTFAKSMASIGGFIASTENVIEYLRYNMRSQIFAKALPM
                     PIVMGALKRLELLRTQPQLKNNLWEIVYALQNGLRQAGFDIGKTESPVTPVYLNGTIP
                     EATNLIFDLRETYDIFCSMVVYPVVPKGIIMLRLIPTAVHTIDDVNYTIECFKKIKVN
                     LENGKYNVNKIVSI"
     gene            1420..1587
                     /locus_tag="PLO59_03235"
     CDS             1420..1587
                     /locus_tag="PLO59_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00144.1"
                     /translation="MATKKAAKKAAPKKAAAKKAAPKKAAAKKAAPKKAAPKKAAPKK
                     AAAKKAAPKAK"
     gene            1752..>2740
                     /locus_tag="PLO59_03240"
     CDS             1752..>2740
                     /locus_tag="PLO59_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00145.1"
                     /translation="MKYFYMSLIILFLNSVSTIAQTITLDFKKALVKENFDSSTSAWT
                     IAANNENFFIVQDGEYILNRKNSVAPFAIVCGFENTALQYKLITSLKLDKASTELNSM
                     GLLFQLQPDKKGGFLFEVNGQKQFRIREISGNGYKFISGDLKGEGWVRSNFIHQNGIA
                     NLIELRFMEGVYDFYINTNFAFTCKNISYKSGNFGFVIGPSSKAKIDFFYLLTNDSNG
                     NVSAKPEDDISILAESIINLKTLLNNVSLENELLINTIEAMKQEAKHFNQEKQQLTTA
                     AEQHQLAYNKLLLVNDSLIRVNNDLNKYKAIFDSNNTGDIVINLSKALKAEKQ"
BASE COUNT          976 a          407 c          516 g          841 t
ORIGIN      
        1 taaaaaagaa cagaggccca ttaggtacac atgcaaaaga atcgcatggg tattttacat
       61 ttccaaagtt ggaaggcgat ataagtaata aaatgatttt tcgtggaaaa gaaaaattag
      121 tttggagcct aaataattat ttaggattgg ccaatcatcc cgaagtgcgt aaagccgatg
      181 ccgactcgag tagcaattat ggaatggctt tacccatggg tgcacgtatg atgagtggca
      241 actctaacta tcatgaaaaa ttagaaaacg aaattgcgca attcatgcaa aaagaagatt
      301 gcttgttgtg caactttagt taccaggcaa tggtaagttc aattgatgct ttggttgaca
      361 gacatgatgt aattgtttac gacagcgaat cgcatgcatg tataatagat ggtttgcgtt
      421 tacacatggg caaacgtttt gtttatccac ataataatat cgaaaatcta gagaagcaat
      481 tagagcgtgc aaccaaattg gttaacgaaa gtggtggatg tattttagtt attaccgaag
      541 gtgtttttgg tatgagtgga gatcagggaa aattaaaaga aattgttgac ttgaaatcga
      601 aattcaattt cagattattt gttgatgatg cacatggatt tggtaccatg ggtgcaaccg
      661 gtgcaggtac tggtgaagca caaggcgtgc aatcaggtat tgatatttat ttcggaacgt
      721 ttgcaaaatc aatggcttcg attggtggat ttattgcttc aacagaaaat gttattgaat
      781 acttgcgata caacatgcgt tctcaaattt ttgccaaagc attacccatg cccattgtga
      841 tgggtgcatt aaagcgatta gaattgctgc gtacacaacc acagctaaaa aataacttgt
      901 gggaaattgt ttatgcatta caaaatggat tgcgtcaggc aggttttgat attggtaaaa
      961 ccgaatcgcc tgtaacacct gtatatctta atggtaccat acccgaagca acaaatttaa
     1021 tttttgattt gcgcgaaacg tatgatatat tttgttcgat ggttgtatat cctgttgtgc
     1081 caaaaggaat tataatgttg cgattaattc ctaccgctgt gcatacgatt gatgatgtaa
     1141 attataccat cgaatgtttc aaaaaaataa aagtgaattt agaaaacgga aaatataatg
     1201 ttaataaaat tgttagtatt tagtagttga aaatttgtgt tcaaaaaagt aatcatatac
     1261 atttgacaac agcaaaacat aaattgttct ttgaaaaagt tttgcaatga tttataaaat
     1321 actaatcaac atatagagta actgttacaa tttaaaattg aaacagcaaa acgcaacagt
     1381 aatagtactg aagcataacg taactaaatc tttaacacaa tggcaactaa aaaagctgca
     1441 aagaaagctg ctcctaagaa agctgctgct aagaaagccg ctcctaagaa agctgctgct
     1501 aagaaagctg ctcctaagaa agctgctcct aagaaagccg ctcctaagaa agctgctgct
     1561 aagaaagctg ctcctaaagc taagtaattg cttctggagt tttcaaaaac acaaggtcat
     1621 gcattgcatg acctttttta ttttatgcct gttagtatta gttctatttt ttttacggca
     1681 atttaaggta acattattat ttctgtttat ttgtaatgca taagttatta cgatacatgc
     1741 attaccggga aatgaaatat ttttacatga gtttaattat acttttttta aatagtgtat
     1801 caacaattgc acaaacgatt actctagatt ttaaaaaggc gttagttaaa gaaaactttg
     1861 acagcagtac ttcggcctgg acgattgctg ccaacaacga aaactttttt attgtacagg
     1921 atggtgagta tattctaaat agaaaaaact cggtagcgcc attcgcaata gtatgtgggt
     1981 tcgaaaacac agctttacaa tataagttga ttacatcatt aaagctggat aaagcttcta
     2041 ccgaattaaa tagtatgggt ttattatttc aattgcaacc tgataaaaag ggtggttttt
     2101 tatttgaagt taatgggcaa aaacaattcc gtatccgcga aatttcgggc aatggctata
     2161 aatttatttc gggcgattta aaaggagaag ggtgggtaag aagtaatttt atacatcaaa
     2221 atggcattgc taatttaatt gaacttaggt ttatggaagg tgtttatgat ttttatataa
     2281 ataccaactt cgcatttact tgcaaaaaca taagttacaa atccggtaat tttggttttg
     2341 taattggacc atcaagtaaa gctaaaattg attttttcta tcttcttacc aatgatagta
     2401 acgggaatgt tagcgctaaa cccgaagacg acattagtat cttagccgaa tcaattatta
     2461 acctaaaaac gttgcttaat aatgtgagtt tagaaaatga gctgcttata aatacaattg
     2521 aagcaatgaa acaagaagcc aaacatttta atcaggaaaa acaacagtta accacagcag
     2581 ctgaacaaca tcaattagct tacaataaat tattattagt aaacgactca ctaatcagag
     2641 tcaacaatga tttaaataag tacaaagcaa ttttcgacag taataataca ggtgatattg
     2701 ttatcaattt atcgaaggca ttaaaagccg aaaaacaaaa
//
LOCUS       DAOMAA010000176         2726 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_31408_length_2726_cov_2.631224, whole genome shotgun sequence.
ACCESSION   DAOMAA010000176 DAOMAA010000000
VERSION     DAOMAA010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31408_length_2726_cov_2.631224"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..476)
                     /gene="ruvX"
                     /locus_tag="PLO59_03245"
     CDS             complement(48..476)
                     /gene="ruvX"
                     /locus_tag="PLO59_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HQV00146.1"
                     /translation="MARLLAIDFGLKRTGIAVTDVLQLIPHALTTVDTNELLTFLETY
                     AKAEEVEKIIVGQPLQKNFKPSEIEPNILSFLGRLKKKLPDIPVVRFGEQHTSQWAMQ
                     SLISSGVKKSERRNKALIDKVSATIILQQYMDAHTNTNLF"
     gene            584..1822
                     /locus_tag="PLO59_03250"
     CDS             584..1822
                     /locus_tag="PLO59_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020601589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HQV00147.1"
                     /translation="MLKKIPLTGWIFIALFLGAAVGEIYRNNHPDIATQRAFAENISV
                     LSDVFLRLIKMIIAPLVFSTLVVGIAKLGDFKVVGRIGIKTILYFQFATLIALALGLV
                     IVNVMRPGDYMQMPLPDAATSGINATAPTLKSFVTHVIPKSVVEAMANNEILPIVIFS
                     IFFGIALASLGAMGKSLIHALDALAHVMFKVTNYVMYFAPLGVFGAICAVITTQGIGI
                     IKGYVYLIACFYGGLIFFAIVVLGSIALLVGIKFGKLLKIIQEPLLLAFSTASSEAAF
                     PKMVEKLKAYGCSDRIISFVLPLGYSFNLDGSIMYMTFATAFIAQTYGRVLSIEEQIT
                     MMLILLVTSKGMAGVPRASLVVIAALLPQFGLPEAGLLLLLGIDQILDMGRSATNVVG
                     NAVATAVVSKWEGELQENHL"
     gene            1835..2701
                     /locus_tag="PLO59_03255"
     CDS             1835..2701
                     /locus_tag="PLO59_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008634410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HQV00148.1"
                     /translation="MQESELILNTDGSIYHLNLLPHDIADIVINVGDPDRVHVVSKFF
                     NHIEIVKQKREFVTHTGYYNGRRMTVISTGIGTDNIDIVYNELDALVNVDLKSRKVKE
                     QLSPLYLIRIGTSGALQADVDVDSFVFSTHGLGLDGLMNFYHYRNNAEEEKILQHFQA
                     HYPHYNTYAQSYLFQCSPVLNQLFSDSFHKGITASCCGFYAPQGRMIRAQIARDGMVD
                     LLSDFSFEKYRITNFEMETSAMFGLARVLGHHCASANLIVANRITKKFSKNYQASLDV
                     MIENVLHKLATL"
BASE COUNT          781 a          453 c          547 g          945 t
ORIGIN      
        1 ttttacgtaa aacggcatca ccaaatgcca caatgggaag tatcatatca aaataaattt
       61 gtgttagtgt gtgcgtccat atattgttgt aaaataatgg tggcacttac tttatcaata
      121 agcgctttat ttcttcgttc cgacttttta acgccagaag atattaagct ttgcatagcc
      181 cattgcgatg tatgttgttc gccaaatcta accacaggta tatcaggcaa tttttttttc
      241 agccttccta aaaatgataa aatgtttggc tctatttcgc tgggtttaaa gtttttttga
      301 agtggctgcc ctacaattat tttttcaact tcttcggctt tggcataagt ttcaaggaat
      361 gtaagcagtt cattggtatc aacagtagtt aatgcatgag gtattaattg caaaacatcc
      421 gtaacggcta tgccggttcg ttttaatcca aaatctattg ctagtaagcg tgccatattg
      481 ggtgcaaagc tataattaag caggtaattg ttattgccta tctaaatgat gctacttttt
      541 atcactttaa aaattatact tacagcgttt aatatttgta cgcatgctaa aaaaaattcc
      601 tttaaccggt tggattttca ttgcactttt tttaggtgct gccgttggcg aaatttatag
      661 aaacaaccat cctgatatag caactcaaag agcttttgcc gaaaatatat cggttttaag
      721 tgatgtgttt ttgcgcttaa taaaaatgat tattgcgcct ttggtgttta gtactttagt
      781 tgttggcatt gccaagttgg gcgattttaa agtagttggt cgcattggta ttaaaaccat
      841 tttatatttt cagtttgcaa cacttatagc attggcattg ggcttggtaa tagttaatgt
      901 tatgcgtccg ggcgattaca tgcaaatgcc gttgcccgat gccgccacca gtggtattaa
      961 tgcaactgca cctactttaa aaagttttgt tacgcatgta atacctaaaa gtgttgtcga
     1021 agcaatggct aacaatgaga tattgccaat cgtaattttt tcaatatttt ttggtattgc
     1081 attggcatca ttaggcgcta tgggcaaatc gttgattcat gcactggatg ctttggcaca
     1141 tgtaatgttt aaagtaacca actatgtaat gtattttgca ccattgggtg tatttggtgc
     1201 aatttgcgct gttattacca cacaaggaat tggtattata aaaggatacg tttacttaat
     1261 agcctgcttt tatggtgggc taattttttt cgctattgtg gtactgggtt ccattgcctt
     1321 attggtagga attaagtttg gcaagctgct taaaataatt caggagcctt tattgcttgc
     1381 tttttcaaca gccagcagcg aggccgcttt tccaaaaatg gtagaaaaat taaaagccta
     1441 tggttgtagc gatagaatta taagttttgt attgccctta ggttattcgt ttaatttaga
     1501 tggcagcatt atgtacatga cttttgcaac agcttttatc gcccaaactt atggcagggt
     1561 attatccatt gaagaacaaa ttaccatgat gctcatttta ttagttacca gtaaaggtat
     1621 ggcaggtgtg ccacgtgcat ccttagtcgt tattgctgcg ctgttaccac agtttggctt
     1681 acccgaagcg ggcttgcttt tattattagg cattgatcaa attttggata tgggccgtag
     1741 tgctactaat gtggtgggca atgcagttgc tactgctgtt gtttcgaaat gggaaggcga
     1801 gctacaagaa aatcatttat aaactattat aatcatgcag gaaagcgaac taattttaaa
     1861 tactgatggc agtatttatc atttaaattt attgccgcac gatattgccg acatcgtaat
     1921 aaatgtaggc gaccctgacc gcgttcacgt agttagtaaa ttttttaacc atattgaaat
     1981 tgtaaaacaa aaacgcgaat ttgttacgca taccggttat tataatggca gacgtatgac
     2041 cgttattagc accggcattg gtaccgataa tattgatatt gtttataatg aattggatgc
     2101 attggtaaat gtggatttaa aatcacgtaa agtaaaagaa cagttatcgc cactgtattt
     2161 aattcgtatc ggaacttcgg gtgcattaca ggcagatgtt gatgtggata gttttgtttt
     2221 ttcaacacat ggtttaggtt tagatgggtt aatgaatttt tatcattacc gtaacaatgc
     2281 agaagaagaa aaaatattac aacactttca ggcgcattat ccacattaca atacctatgc
     2341 acaatcttat ttattccaat gttcgcctgt gttaaatcag ctttttagcg actcgtttca
     2401 caaaggcata acggcatcgt gctgtggttt ttacgctccg caaggccgta tgatacgtgc
     2461 acaaattgca cgcgatggca tggttgattt attaagcgac tttagttttg aaaagtatcg
     2521 cattaccaat ttcgaaatgg aaaccagtgc catgtttggg ttagcgcgtg tattgggtca
     2581 tcattgcgca tcggcaaatt taattgttgc aaatcgtatc actaaaaaat tcagcaaaaa
     2641 ttatcaggct tcattagatg tgatgattga aaatgtatta cacaaacttg ctacattatg
     2701 agtaaccttt ttcgttttat agtgtg
//
LOCUS       DAOMAA010000177         2721 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_31530_length_2721_cov_2.952363, whole genome shotgun sequence.
ACCESSION   DAOMAA010000177 DAOMAA010000000
VERSION     DAOMAA010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31530_length_2721_cov_2.952363"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="PLO59_03260"
     CDS             complement(<1..546)
                     /locus_tag="PLO59_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00149.1"
                     /translation="MYFAHTNATEQLISSYKPVQPFINYLKDFFKKNKKMGASDRRTI
                     GNYCFNYFRWNNALPNLSFVENLQLALYAIDNVKHPNLPHYNLLSQRLEAIKNTYPAF
                     SLFNINNTSTYFSKAINVTDALYYQLTKPMVFVRIVNKADLVKTELTKNNIAFIEIDT
                     KTLSIASAAKLTQLQVYADGCF"
     gene            complement(563..877)
                     /locus_tag="PLO59_03265"
     CDS             complement(563..877)
                     /locus_tag="PLO59_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00150.1"
                     /translation="MKILMSIIMFFISASLFGQTDSLPETISDKIYKFDLRFPAQCID
                     KIEYSKSKRKVKYELSEDGRTVYIKEHVIGNGVKVECTYRDGNRDVFTKSTCALELVV
                     PL"
     gene            961..1365
                     /locus_tag="PLO59_03270"
     CDS             961..1365
                     /locus_tag="PLO59_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008238065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase
                     family protein"
                     /protein_id="HQV00151.1"
                     /translation="MQTLASTINKVKQFHEVFGLPSNETPTAHVDEKIYTLRYNLMKE
                     ENEEYLEAAQQGDLTQIADALGDKLYILCGTIIAHGLQHKIAEVFNEIHRSNMSKLDA
                     NGKPVYRSDGKILKGENYFLPDIDSVLNANSY"
     gene            1575..>2721
                     /locus_tag="PLO59_03275"
     CDS             1575..>2721
                     /locus_tag="PLO59_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQV00152.1"
                     /translation="MALQQANHLQSDSIRLYALRTCVQAYLNKPYAEDSALVYIPEIE
                     NLLTRNKFYTLHISCCQTVASYYTFLHRYDDAIRFVQLGLDIAKSNGTITQQSQMRIA
                     EGEIYRKSNAFDFAKLAFTEAGRLAVIDNNSQNQISANRGLGICYDMTKDYQNAEKYF
                     GLALQAALLDSNEKMHHGSLGNYAVAKMHLDKYDEAIVYFKWAVRLAEKYFKPKLITE
                     YKNLADVYLKKKACDSSLYYGNLSLQIAIERNEKLMQTIVYELLDEASSCLGNYQDAY
                     KYAKLGSMVSDTLKAKEKDNAIAKAIATYKYKEQVAEINLLNADKNLQQLKLKQQQNE
                     LELANLYNLKSADAIKILNQDKAISDLKLHENEINMHAQTLKITNTSI"
BASE COUNT          934 a          425 c          496 g          866 t
ORIGIN      
        1 gaaacaacca tcggcataaa cttgtagttg tgtaagctta gctgcactgg ctatacttaa
       61 ggtttttgta tcaatctcaa taaatgcaat attgtttttt gtcaattccg tttttaccaa
      121 atcggcttta tttactatgc gaacaaaaac cattggctta gttaattgat aatacaaagc
      181 atcggttaca ttaattgctt tactgaagta tgtcgaagtg ttattgatat taaataaact
      241 aaaggcagga tatgtgtttt taatggcttc caacctttgg ctcaataaat tgtaatgggg
      301 taagttaggg tgttttacat tatctatggc atacagggct aactgtaaat tttcaacaaa
      361 acttaagttt ggcaatgcat tattccatct aaaataattg aagcaataat tgccgatagt
      421 gcgcctgtcg gaagcgccca tttttttgtt ttttttaaaa aagtctttta aatagtttat
      481 aaatggctgt acaggtttat atgatgatat taactgctct gtagcattag tatgggcaaa
      541 atacataaac aatatccaat atttataatg gtactaccaa ctctaaagcg caggttgatt
      601 tggtaaacac atctctgttg ccatctctgt aagtacattc cacttttaca ccgttaccaa
      661 taacatgttc cttaatatac acggtgcggc catcttccga taattcatat tttacttttc
      721 gtttgctttt actatactca attttatcaa tgcactgagc cggaaaacgc aagtcgaact
      781 tgtaaatttt atcggaaatg gtttcgggta aactatcggt ttggccaaac aaggatgccg
      841 aaataaaaaa cattattatt gacattaaaa ttttcatgtg ggcgcgtttt tatggtaaat
      901 ttataaaatt gattaattcg aaccactttt tatacataca cacttaatta aaaaatattt
      961 atgcaaacat tagcttctac tatcaataag gtaaaacaat ttcatgaggt ttttggcctg
     1021 ccaagcaatg aaacgcctac agcccatgtt gacgaaaaaa tatacacgtt gcggtacaac
     1081 ttaatgaaag aagaaaatga agagtatttg gaagccgcac aacaaggtga tttaacgcaa
     1141 attgccgatg cccttggcga taagttatat atattgtgtg gaaccattat tgcccatggt
     1201 ttgcagcata aaattgccga agtgtttaac gaaatacatc gaagtaatat gagtaaatta
     1261 gatgcaaacg gaaaacctgt ttaccgcagc gatggcaaaa tattaaaggg tgaaaattat
     1321 tttttgccgg atattgattc ggttttaaat gcgaattcat attagttttt taagctttta
     1381 agtagttgct tttggtaatg aagaatacga aatgccttgc ttcttttttt gttgtacttc
     1441 atttggctct gctttgtagc atagcggttt tttgtcagtc ggaaaatatt cgcaaaaacc
     1501 aatcggatgc ggtttcatta tgtgaactaa gcagattaag caccagtcgg gattcaagca
     1561 ttttatatgc aaaaatggca ttacaacagg ccaaccattt gcaaagcgat tcaataaggc
     1621 tgtatgcgtt acgaacttgt gtacaagctt atttaaataa accgtatgcc gaagattcgg
     1681 cattggttta tatacctgaa attgaaaatc tgcttacgcg aaataagttt tatacgctgc
     1741 atatttcatg ttgtcaaaca gttgcaagct attatacttt tttgcacagg tatgatgatg
     1801 caatacggtt tgtacaactt ggattggata ttgcaaaaag caatggtacc ataacacaac
     1861 aaagccaaat gcgtattgcc gaaggggaga tatatcgaaa atcgaatgca tttgattttg
     1921 ccaaacttgc atttaccgaa gcaggacgtt tagcggttat tgataataat tctcaaaatc
     1981 agatttcggc aaaccgtggc ttgggtatat gctatgatat gactaaagat taccaaaatg
     2041 cagaaaaata tttcggcctt gcattacaag cagctttgct ggatagtaat gaaaaaatgc
     2101 accatggctc attgggtaat tatgcagtag caaaaatgca cctcgataaa tatgacgaag
     2161 ccattgttta ttttaaatgg gcggttcgct tagctgaaaa atattttaag ccaaaattaa
     2221 ttacagagta taaaaactta gctgatgttt atttaaaaaa gaaggcatgc gatagctctt
     2281 tatattatgg caatttatca ttacaaattg caattgaaag aaatgaaaag ctaatgcaaa
     2341 ccattgtgta tgaactgtta gatgaggcca gcagttgttt gggtaattat caggatgcat
     2401 ataagtatgc taaattggga agcatggttt ccgacacact taaggccaag gaaaaggata
     2461 atgccattgc taaagctatt gctacgtata aatataaaga acaagttgcc gaaattaatt
     2521 tgttgaatgc agataaaaat ttgcaacaat taaaattaaa gcaacaacaa aatgaattgg
     2581 aattagccaa tctgtataac ttaaaatcgg ctgatgctat taaaatttta aatcaagaca
     2641 aagcaataag cgatttaaaa ttgcatgaaa acgaaattaa tatgcatgca cagacattaa
     2701 aaattacgaa tacaagcatt g
//
LOCUS       DAOMAA010000178         2718 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_31592_length_2718_cov_2.636876, whole genome shotgun sequence.
ACCESSION   DAOMAA010000178 DAOMAA010000000
VERSION     DAOMAA010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31592_length_2718_cov_2.636876"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="PLO59_03280"
     CDS             <1..533
                     /locus_tag="PLO59_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HQV00153.1"
                     /translation="GKRLWQCNLYSCRFRERCDDDKETMTLYDSLGGTKMVADPVGGA
                     MIEQGRLGIRSVVDSTIFVIAADTQLNTKFFSVLLAEVGAGNLTGFNILSKNLTDFFC
                     VATGAKNYTYNGLDMVSAHNPATHSRIGSKINNAEFDRFVADLVTGAQKVGLSNQLIG
                     QVGALVETTRSQVVQN"
     gene            complement(682..2094)
                     /locus_tag="PLO59_03285"
     CDS             complement(682..2094)
                     /locus_tag="PLO59_03285"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HQV00154.1"
                     /translation="MTYSYLKIVLSTCMLISASIANAQTDDVLNLLINKNVISQNDAD
                     SMRAEAAIQGQQKHVDKVFSIDLEFRPRTEYRQGYGQMSNDTTASAFYTNQRTRISFG
                     YQILDKLNFQFSIQDIRTFGKDDPRTNAGTIQVFEAYLEPYITKNFSIRVGRQRLMYD
                     NQRLFAQNDWRQNANAHDGINFRYINTKFKTELAGVWNQSAEATAGTGFFPVDQTGKP
                     IVNYKLLVVNYLNWNINKQFTLTTINCADGFQAPTNVEKLYTRFTNGGRIEFEHESIY
                     ATFSAYYQHGKNNTGQKLNAFYIQPELKYLHKNTTVRLGAEIMSGQDATKTSNEANSF
                     VPLYGVAHRFNGFMDLYTRFPGDLNYAGLVNPYLFIIQNISKKVNVGFFFHQFYSQNN
                     FVDSDNNVIDKNLGFEHDILFQYKPNNFTTLEVGYSYYLPTESTAIIKKAKVGAEKEW
                     QQWAYVMLIVKPQLFKHKFN"
BASE COUNT          789 a          432 c          575 g          922 t
ORIGIN      
        1 tgggtaaaag gttatggcaa tgtaaccttt atagctgccg atttagagag cgttgcgatg
       61 atgataaaga aaccatgaca ttgtacgact cattgggtgg tacaaaaatg gtagcagatc
      121 cggttggggg tgccatgatt gaacaaggca ggttaggtat aaggtcggtg gttgatagta
      181 ccatatttgt aattgctgcc gacacgcaat tgaacactaa attttttagt gtattgttag
      241 ccgaagtagg tgcaggtaac ctaacaggat ttaatatact tagtaaaaac cttaccgact
      301 ttttttgtgt ggctacagga gctaaaaatt atacctataa tggcttagat atggtatcgg
      361 ctcataaccc tgccacacat agtcgtatag gttcgaagat aaacaatgca gagtttgatc
      421 gctttgttgc cgatttggtt accggtgcac aaaaagttgg actaagtaat cagttaatag
      481 gtcaggttgg cgccctggta gaaacaacca gaagccaggt ggtacagaac taaaatgatt
      541 ttatgagttt gttggagttt tattattaaa gagccaatgc ttttgcattg gttctttaat
      601 aatattggta tgattattcc gaaaattgaa aggcataaat agtaagtagg ccgcttactt
      661 taactgcagt tcattgaatg cttaattaaa tttatgctta aacaattgcg gctttacaat
      721 taacattacg taagcccatt gctgccattc tttttcagcg cctacctttg ccttttttat
      781 aatggctgtt gattctgtag gcaggtagta tgagtatcct acttctaaag tagtaaagtt
      841 gttaggctta tattggaaaa gtatatcatg ctcgaagcct aagtttttgt caattacatt
      901 gttgtcagaa tcaacaaagt tgttttgaga ataaaattga tgaaagaaaa atccaacatt
      961 aactttcttc gaaatgtttt gtatgataaa caaataggga ttaaccaaac ctgcatagtt
     1021 taagtcgccc ggaaagcgtg tatataaatc cataaagcca ttaaagcgat gtgctacacc
     1081 ataaagtggt acaaaggagt tggcttcatt actggtttta gttgcatcct ggccgctcat
     1141 aatctcggca cctaaacgta cggtggtgtt cttgtggagg tatttaagtt cgggttgtat
     1201 gtagaatgca tttagttttt gacccgtatt atttttacca tgctggtagt aagcactaaa
     1261 agttgcgtaa atgctttcat gctcaaattc aatacgtcca ccatttgtaa agcgtgtata
     1321 taatttttca acattggtgg gtgcctgaaa accatcagca cagtttattg tagtaagcgt
     1381 aaactgttta ttaatattcc agtttaaata atttacaact aataatttgt agttaacaat
     1441 gggttttcct gtttgatcaa caggaaaaaa tccggtaccg gctgtagctt cggcactttg
     1501 attccacacg cctgctaact ccgttttaaa ttttgtattt atataacgga agttgatacc
     1561 atcatgtgcg ttggcgtttt ggcgccagtc attttgtgca aatagccgtt gattatcata
     1621 catcaaccgt tgacggccta cgcgaattga aaaatttttg gtgatatatg gttctaagta
     1681 agcttcaaaa acttgaatgg tacccgcatt ggtacgtggg tcgtctttac caaaggtgcg
     1741 tatatcctga attgaaaact ggaaatttag tttatccaat atctgatagc caaatgaaat
     1801 acgcgtacgt tggttggtat aaaatgccga tgccgttgta tcattagaca tttggccata
     1861 accttggcga tactctgttc gtggcctgaa ctccaaatca atgctgaaaa ctttgtcaac
     1921 gtgtttttgt tgaccttgga tagcagcttc agcacgcata gaatctgcat cattttgtga
     1981 gataacattt ttgttaatga gtaggtttaa tacatcatct gtttgcgcat ttgcaatgct
     2041 tgcacttata agcatgcagg tacttaaaac aatttttagg tagctgtagg tcatatgttt
     2101 ttaaattttt atgctgcgaa taaaacaaaa atctattaaa tctatcaaac aagtagatta
     2161 attattaaat tttttttttc ttaccacctt tagatactgt tttttttcaa acactgcttt
     2221 gctgtttggc ttgtctaaca aaatggtggt taacagcact taggatttat aaagccaaat
     2281 tgattagaga aagcccttaa ataaaattat ccggtactaa tttgattttt acttatagcg
     2341 catagtaatt ggttgaattt aaagccattt tagcaagtca gatttgtaca gcgttatggt
     2401 gcctatgaag tgttgggtag ttagtagttc tgaaaagtaa tgcatgcata cagtttatgc
     2461 gaaaagtatt gatgctggtt ctagtagcta acatcatata gtgtaagtgt aatagcaaaa
     2521 aaaatcccac aattgtggga ttttactttt aacgtggatg gcgattgcta cccgatggtc
     2581 gttgtgcagg tgcactgggt cttgattgcg gggctgatgg ttttgttgta ggtgatgtgg
     2641 gccgtgtttt tggtgcattg ggttttatat cattattacg gcttggtaaa tcgggtcttg
     2701 tttgcgcagg tgtttccg
//
LOCUS       DAOMAA010000179         2717 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_31627_length_2717_cov_2.063110, whole genome shotgun sequence.
ACCESSION   DAOMAA010000179 DAOMAA010000000
VERSION     DAOMAA010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31627_length_2717_cov_2.063110"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1247)
                     /locus_tag="PLO59_03290"
     CDS             complement(<1..1247)
                     /locus_tag="PLO59_03290"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQV00155.1"
                     /translation="MPVLLLGIIVIIFVAFGIRLLIPGKPANAILWQSKQGMGNFNEN
                     KNMATIEAAMGQHLPLFYISFELAVIPNDLQNLAPDKHHKPLRKIISMHGYSKALWQY
                     YAACKDMAALSSDPMLQPFLDSFYSAFEKANMPLQQQALKNIILNSRENEANQCAMQL
                     LNLNRQVQTGKTTLFDFLPVLNYHKQNQFHNWLLGAANQKGVLSGNFGNSFTTQQPIN
                     DTLKQRLTWSVALALLAFIVTLLFALPLGIYTAINPQSVLCKLLQFYQTLSTTIPLFI
                     FATLMLLFFANDDWFGLFPASVHSTNNLSLFWFLLLPLACYCFEGIAYTSQIIATQMQ
                     QTLKQMHVVYAKTLGFNQAYILKKHVLPAICITLLSVFLQWIPAAFAGSLIIENVFGI
                     PGIGFEMAQSVYTQNYPMLSSLV"
     gene            complement(1291..>2717)
                     /locus_tag="PLO59_03295"
     CDS             complement(1291..>2717)
                     /locus_tag="PLO59_03295"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQV00156.1"
                     /translation="WDNGQPLTVADIIFTAKAHACAYTQNPNAKPYWDMVNDIIVDIE
                     NALKFTVVMKQAHIQNVSMWCDYPIIQQSLYDTKQVLNKFSFDQLRNDTSNKEIETWA
                     NQFNQINFRDVKNVSGLGDYKLATWDAGIRLTLVRKTQSVRTTLPLADTLHFAISSDA
                     TAQILEFKAQTFDASTTLSTKTLLMLQKDSAFNTNYHSAFVSTFNYTYAAFNCKPDEN
                     HQPFFTDKNVRRALALLTPVDQINKVLNQNKNKRLATPVAPVKDECNKILEILPFDVN
                     QAKELLNKSGWIDSNQNGIRDKLIDGKLVEFKFELLYFTNSPDWKDYSEMMATSYLSA
                     GVEAVPTGYDPLTGQQKMFAQNFDMLLSSWGGNAVPEDYTQLWSTQAWQQKGANFTGF
                     GNAFTDSVITAISQCMETEKRNNLSKQLQTIIYDEQPYVFLFTSMRKIAVHKRISNIE
                     LYFERPNLLLNQLQLKINNPKTIQ"
BASE COUNT          842 a          485 c          497 g          893 t
ORIGIN      
        1 aaaaccaaag atgataacat cggatagttt tgggtgtaaa cggattgcgc catttcgaaa
       61 ccaatgcccg gtatgccaaa tacattttca ataattaagc ttccggcaaa tgcagccgga
      121 atccattgta gaaatacaga tagaagtgta atacaaattg ccggcaaaac atgttttttt
      181 aaaatgtaag cttgattaaa gcctaatgtt tttgcatata ccacatgcat ttgctttaaa
      241 gtttgttgca tttgtgtagc aataatttgt gatgtataag caatgccttc aaaacaataa
      301 catgctaagg gtaatagcag aaaccaaaac aaacttaagt tattagtgct atgcacactt
      361 gccggaaata acccaaacca atcatcattt gcaaaaaaca gcagcattaa tgttgcaaaa
      421 ataaacaaag gtattgtggt agataaggtt tgataaaact gcaacaactt acataaaacc
      481 gattgcggat taatggcagt ataaattccc aaaggcagtg caaatagcag tgtaacaata
      541 aaggccaata atgctaaagc tactgaccag gttaaacgtt gttttagcgt atcgtttata
      601 ggttgttgtg tggtaaatga atttccaaaa ttacctgata atacaccctt ttgattggca
      661 gcacctaaca accaattatg aaactggttt tgcttatgat aatttaatac cggtaaaaaa
      721 tcaaagaggg ttgttttacc agtttgcact tgcctattaa gatttagtaa ttgcatagcg
      781 cattgattcg cttcattttc acgcgaatta agaataatgt ttttcaatgc ttgttgttgt
      841 aaaggcatat tggctttttc gaaagcacta taaaatgaat ccaaaaaagg ttgtaacatg
      901 ggatcagatg ataaggcagc catatcttta caggcagcat aatattgcca taaggctttc
      961 gaatagccgt gcatgctaat aattttgcga agcggtttgt gatgtttatc cggagccaaa
     1021 ttttgtaaat cattcggtat tacagcaagt tcaaaactga tataaaacaa aggcaaatgt
     1081 tgccccatag ctgcttctat agtagccatg tttttatttt cgttaaagtt gcccatgcct
     1141 tgttttgatt gccatagtat agcattggcg ggcttgccgg gtatcaatag tctgatacca
     1201 aaagcaacaa atattataac aattatacct aaaagaagta ccggcaaccg gcaccaaaaa
     1261 aggtaaaaaa attgcctgat cagcatttca ttattgaatt gttttcggat tatttatttt
     1321 cagttgcaat tgattaagca ataaatttgg tcgttcgaag taaagctcaa tattgcttat
     1381 tctcttatgg acagctattt ttcgcatgct ggtaaataaa aacacataag gctgttcatc
     1441 ataaataata gtttgtagtt gtttcgacaa attgttacgt ttttcggttt ccatacattg
     1501 gcttatggca gtaataacgc tatcagtaaa cgcattgcca aagcctgtaa aattagctcc
     1561 tttttgttgc caggcttgcg tactccacaa ctgtgtatag tcttcgggga ctgcattgcc
     1621 accccaacta ctcagcagca tatcaaaatt ttgcgcaaac attttttgtt gtccggttaa
     1681 tgggtcataa cctgttggta cagcttcaac acctgcactt aaataggatg ttgccatcat
     1741 ctcggaatag tctttccaat cgggcgaatt ggtaaaatac agcagttcaa atttaaactc
     1801 aaccaatttt ccatcaatca atttatcgcg tatgccattt tggttactat ctatccaacc
     1861 acttttgttt agcaattctt ttgcttggtt aacatcaaat ggcagtattt cgaggatttt
     1921 attacactca tcctttaccg gagcaaccgg tgtggctaaa cgtttgtttt tattttggtt
     1981 aagtacctta tttatttgat caaccggtgt tagtaaagcc aaagccctac gcacgttttt
     2041 atcagtaaaa aagggctgat gattttcatc cggcttgcaa ttaaaagcag catacgtgta
     2101 attaaatgtg ctaacaaatg ccgagtgata gttggtatta aacgcactat ccttttggag
     2161 cattaaaaga gtttttgtac ttaaggttgt acttgcatca aaagtttgag ctttaaattc
     2221 gagtatttga gcagttgcat cacttgaaat ggcaaagtgc agtgtatctg ccaacggcaa
     2281 cgttgtacgt acactttgcg ttttgcgtac gagtgttaat cttattccgg catcccaagt
     2341 tgctaattta taatcgccca agcctgaaac attttttaca tctctgaaat taatttgatt
     2401 aaattgattt gcccaggttt ctatctcttt attggatgta tcgttgcgta gttggtcaaa
     2461 tgaaaactta tttaatacct gtttggtatc atataaactt tgttggataa tcggataatc
     2521 gcaccacata cttacatttt gaatatgtgc ctgtttcatt acgactgtaa actttaatgc
     2581 attttcaata tctacaatta tatcatttac catatcccag tatggcttgg catttgggtt
     2641 ttgtgtgtag gcgcatgcat gtgccttagc ggtaaaaatt atatcggcaa cagtaagcgg
     2701 ttgcccattg tcccatt
//
LOCUS       DAOMAA010000180         2707 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_31834_length_2707_cov_2.792986, whole genome shotgun sequence.
ACCESSION   DAOMAA010000180 DAOMAA010000000
VERSION     DAOMAA010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31834_length_2707_cov_2.792986"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="PLO59_03300"
     CDS             complement(<1..732)
                     /locus_tag="PLO59_03300"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HQV00157.1"
                     /translation="MRSSSGEPWVNNNNVTLMNTAFGSSGWILGTFQNTAAATVFNNT
                     TKLVFMDGSNLGATALNNYLISNGTVIENWVSNGGMLFINAAPDQGGNFGLGFGGVML
                     NFNTNYNKASNNASISSGQNTHQIFTGPNACGTSWTGVSFAHATITGGAATPLITGNK
                     GTVLAEKISGYGRVLFGTMTLASFHSPQPNATNLRLNIFAYLNNNAPFIVQCTSTNAS
                     CIGNNNGSVSVSVLTGGTTPFTYLWN"
     gene            complement(1248..1439)
                     /locus_tag="PLO59_03305"
     CDS             complement(1248..1439)
                     /locus_tag="PLO59_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014774178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HQV00158.1"
                     /translation="MLQGTVKFYNEKKGFGFIVEEGSNREVFVHATGLIDPIRQADNV
                     TFETSEGKRGINAVNVRKA"
     gene            1694..>2707
                     /locus_tag="PLO59_03310"
     CDS             1694..>2707
                     /locus_tag="PLO59_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016199750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HQV00159.1"
                     /translation="MSQNKTAHPKGLYVLFTTEMWERFNFYGMRAILSLFMVEALMLG
                     EKNASIIYGGFLGLCYLTPMLGGYLSDKYLGNRICILLGGFMMAVGQFILFYSASVFG
                     NDIDFAKALLWTGLLVLIFGNGFFKPNISSMVGQLYPPHEKSKLDSAFTIFYLGINVG
                     ATLGILICPLLGDVKDSLGVRDLTAFKWGFFAAGVAMLLACIIFYLLKDKYVITPEGE
                     KIGDKPDHNKLKASGEEQAKFTTNSLIMSVLILVGLTFAFHYLSFDPNESDPNPIKLW
                     LYPFIYASGISLAYLIASDKSLTKIERDRIFVLYIVALFVMFFWAAFEQAGSSLTFIA
                     DKQT"
BASE COUNT          824 a          465 c          507 g          911 t
ORIGIN      
        1 attccataaa tatgtaaaag gtgttgtgcc tccggttaat acacttaccg aaacacttcc
       61 attgttattg ccaatgcagg aagcgttggt actggtacat tgaactataa aaggagcatt
      121 gttatttaaa taggcaaaaa tgtttaatcg cagattggtg gcattgggtt gtgggctatg
      181 aaacgaagct aaagtcatgg tgccaaatag aacacgacca tatccactta ttttttcggc
      241 taatacggtg cctttatttc ctgtaattaa tggtgtagct gcacctccgg taattgtagc
      301 atgtgcaaaa gaaacgcctg tccaacttgt gccgcaagca ttaggtcccg taaatatctg
      361 atgcgtgttt tggccactgc taatgctggc attattgcta gctttattat aattggtatt
      421 aaagtttaac ataacaccgc caaaacctaa accaaagtta ccaccctggt caggtgctgc
      481 atttataaaa agcataccac cattgctcac ccagttttcg attacggtac cgttacttat
      541 aagataatta tttaatgcag ttgctcctaa gttgctgcca tccataaata cgagtttagt
      601 tgtgttattg aaaaccgttg cagcagccgt gttttgaaat gtacctaaaa tccaacctga
      661 acttccaaag gctgtattca ttagggttac attgttatta ttaacccatg gttcgcccga
      721 agatgaacgc atataataaa cgtttaacgc cttgctttgg gttaaaatta aaagcaataa
      781 aattgagaga agtagaactt ttttcatcat tgattggtaa aatgtgtagt gcttaaatgt
      841 tgtagggtaa actgcacgta ccttaataag gatgtaggca gcttgttgga tcgggggctt
      901 tgttataaaa aacttttgtg ttgttaataa cgttgttgaa aacgcttaaa aatacattgg
      961 caaaatacaa tcaaaaagtg tttggtcaac aaaaaagtag tattcaccta taaaaaaatg
     1021 aatttagcca actggttact ttcagcaagt tgcttaagtg cttgatagtt agtgttttaa
     1081 caaaatgagt tttcttcgta aaagttatga acaaattgaa gctcttttta attttgtttc
     1141 aatcagaaaa agtttggtta ctagaaaatg tgtattgctt aaaataaaaa agccctgtaa
     1201 aaaattacag ggctcattag cggatttaat ttattacaat aattaaatta agcttttctt
     1261 acgttaacag cattaatgcc tcttttgcct tcgctggttt cgaaagtaac attgtcagcc
     1321 tgacgtattg ggtcgattaa acctgtggcg tgaacaaata cttcgcgatt cgaaccttcc
     1381 tcaacaataa agccgaatcc ttttttttcg ttgtaaaatt ttacggtacc ttgtaacatt
     1441 gaataaatta ttaaaattga atttgggcaa aggtaatttt attagtataa ggtacaacat
     1501 gtttcaacag acttaaataa aatcaaatgt gcttaagctg gttttttcgt attgattttt
     1561 gcaacattaa aaaaacaata cattagccgc catctcaaaa atcaatattt taatatgcta
     1621 cagcatgtgt cacttttgaa attaaattga catgaatgct tcaaaacaaa cttaactttt
     1681 taaaaatctt attatgagtc aaaacaaaac tgctcatcca aagggcctct atgtgctttt
     1741 tactaccgaa atgtgggaaa ggtttaattt ttatggcatg cgtgccatac tgtctctctt
     1801 tatggttgag gctttgatgc tgggcgaaaa aaatgcttcc atcatttatg gaggattttt
     1861 aggtctttgt tatctcacac cgatgcttgg cggatatctt tctgacaaat acttaggcaa
     1921 ccgcatttgt attttattgg gtggctttat gatggctgtt ggccagttta tactttttta
     1981 tagtgccagc gtttttggaa acgatataga ctttgcgaaa gcacttttat ggacggggct
     2041 tttggtttta atatttggca acggattttt taagcccaat atctcatcaa tggtcgggca
     2101 gttatatcca ccgcatgaaa aaagtaaact tgattcggca tttaccatct tctatctagg
     2161 aattaatgtg ggtgctacat tgggcatact aatttgtccc ttgcttggcg atgtaaaaga
     2221 cagcttaggt gtacgcgacc ttactgcatt taaatgggga ttttttgctg caggtgtggc
     2281 tatgttactt gcttgtatca ttttttattt gctaaaagat aaatatgtga ttacgcccga
     2341 aggtgagaaa attggcgata aaccagatca taataaactg aaagcaagtg gtgaagagca
     2401 agcaaaattt acaacaaaca gtctgattat gtctgtgctc attttagtcg gtcttacatt
     2461 cgcatttcat tatctttctt tcgaccctaa cgaatctgac ccgaacccaa taaaactttg
     2521 gttgtatcct ttcatttatg catcgggtat tagcttagct tatttaatag catccgataa
     2581 atcacttaca aaaattgaac gcgaccgcat ttttgtactt tacattgtag cattgtttgt
     2641 aatgtttttc tgggccgcat ttgagcaggc cgggtcgtca ctcacattta ttgccgataa
     2701 acaaacc
//
LOCUS       DAOMAA010000181         2704 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_31885_length_2704_cov_2.815780, whole genome shotgun sequence.
ACCESSION   DAOMAA010000181 DAOMAA010000000
VERSION     DAOMAA010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31885_length_2704_cov_2.815780"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="PLO59_03315"
     CDS             complement(<1..938)
                     /locus_tag="PLO59_03315"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQV00160.1"
                     /translation="MQLSYWEQKQYFSKVDFVIIGSGIVGLNAAIQLRKQFKTASILV
                     LERSFLPYGASTRNAGFACFGSMTEILDDLTRMTEDAVYALVEKRYNGLLLLRKLLGD
                     VNIGYEEKGGYEVFTNSDDESFEQCMDAMTDLNTKLKSIVKGNTYTLSDFEISKQGFN
                     GVKHLVKNNFEGQIDTGKMMHALVKLAQNNNITILNGLDIIEVKTLPNGVLITTAAGF
                     SFTTKKLMVCNNGFAANLFPQLEVQANRAQVLITNPIEGLKINGAFHYQKGYYYFRDV
                     DGCVLLGGGRNLDFEAEATQQFGVTEKVQTALEKLL"
     gene            complement(951..1550)
                     /locus_tag="PLO59_03320"
     CDS             complement(951..1550)
                     /locus_tag="PLO59_03320"
                     /inference="COORDINATES: protein motif:HMM:NF003191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HQV00161.1"
                     /translation="MDLTDFYQSNIFHYAVMPLLIFLCRVCDVTLGTLRNLFLAKGIK
                     NVVPFIGFLEVLIWTIAVSQVLKHLDNLLSYTAFALGYATGIYVGIKMDERLGLGLQL
                     FRIVIVKHTQALLDALKQINIGYTLVDGAGSQGPVKLIYLVIKRTHHNQVCNLIHTHN
                     PDAFYTVEDVKDANKGVFPPIGSNQTLYYLRRLFPLFKH"
     gene            complement(1552..2244)
                     /locus_tag="PLO59_03325"
     CDS             complement(1552..2244)
                     /locus_tag="PLO59_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015030875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HQV00162.1"
                     /translation="MKKIVVFTGAGISAESGIKTFRDSGGLWEQHKIEDVATPEAWFR
                     NQKLVLDFYNQRRKNVLEALPNVAHNAIAELQYAFDVHVITQNIDDLHERAGSKKVLH
                     LHGEIIKARSTVKPNLIYPINGWELKEGDTCELGSQLRPHIVWFGEPVPLMETAAVIA
                     SSADVFIVIGTSLEVYPAASLIDYVNANAVKYLIDPNAKSLTHIDNLQTIRDTACNAV
                     PPLIYKLLENVN"
     gene            complement(2247..>2704)
                     /locus_tag="PLO59_03330"
     CDS             complement(2247..>2704)
                     /locus_tag="PLO59_03330"
                     /inference="COORDINATES: protein motif:HMM:NF014908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HQV00163.1"
                     /translation="KLMEAGGALVIKTVETIANGTVKTINQNELSIEPLHDAPKLFKQ
                     HLLINFNKPVTAVYNHIRGLSPYPAAFFEFTTHDQKLYSMKVYGCSYETATVNFDGII
                     YTDNKTYLNIYCLGGILRLTNIQLAGKKRMDTTEFLRGFNMSNCKLIYR"
BASE COUNT          829 a          547 c          431 g          897 t
ORIGIN      
        1 tgcagcaatt tttccagtgc tgtttgcact ttttcggtaa caccaaattg ctgtgtagcc
       61 tctgcttcaa aatctaaatt acgtccgcca cctaataaaa cacatccgtc aacatcacga
      121 aaataatagt agcctttttg ataatggaat gccccgttaa tttttaaacc ttcgattgga
      181 ttggttatta aaacctgggc acggttggct tgtacctcta attgtggaaa taaatttgca
      241 gcaaaaccat tattacaaac cattaatttt ttagttgtaa atgaaaaacc ggctgctgtt
      301 gtaattaata caccattggg tagcgtttta acttcgataa tatctaatcc gtttaaaata
      361 gtaatgttat tgttttgtgc gagttttacc aaagcatgca tcattttacc ggtatcaatt
      421 tgcccttcga aattattttt aaccaagtgc ttaaccccat taaatccttg cttgctaatt
      481 tcaaaatcgg ataatgtata ggtgttgcct ttaactattg atttaagttt agtatttaaa
      541 tctgtcatgg catccataca ttgttcaaag ctttcatcat cgctgtttgt aaaaacttca
      601 taaccgcctt tttcttcata gccaatattt acatcgccca ataattttct taacaacaac
      661 aaaccgttat atcgtttttc tactaatgca taaacagcat cttcagtcat acgggttaag
      721 tcgtctaaaa tttcagtcat gctgccaaag caggcaaagc ctgcattgcg tgtactggca
      781 ccataaggca aaaaactccg ttcaagcacc aaaattgaag ccgttttaaa ttgcttgcgt
      841 aattgtatag ctgcgtttaa acccacaatt ccactgccaa ttatcacaaa atcaaccttc
      901 gaaaaatatt gtttctgttc ccagtaactt aattgcattg tatatgtttt ctaatgtttg
      961 aataaaggaa ataacctgcg caagtaatac agtgtttgat tactaccaat gggcggaaac
     1021 acacctttgt tggcgtcttt tacatcttca accgtataaa atgcatcggg gttatgtgta
     1081 tgaattaaat tacaaacctg attgtgatgt gtgcgtttaa ttaccagata tattaattta
     1141 accgggcctt gactgcctgc accatccacc aatgtataac ctatgtttat ctgtttcaaa
     1201 gcatctaaca atgcttgtgt atgttttaca attacaatgc gaaataactg caaacccaag
     1261 cccaatcttt catccatttt aatgcctaca taaataccgg ttgcataacc caatgcaaag
     1321 gctgtataac taagcagatt atctaaatgc tttaaaacct ggctcacagc aatagtccaa
     1381 attaacacct ctaaaaaccc aataaagggc accacatttt taatgccttt ggctaaaaac
     1441 aaatttcgta aagtgcccaa tgtaacatca caaactctgc ataaaaaaat aagcaagggc
     1501 attacagcgt aatgaaatat attgctttga tagaaatcag tcagatccat attagtttac
     1561 attttcaagc aatttataaa tcaggggtgg cacggcattg caggcagtat ccctaatggt
     1621 ttgcaaatta tcaatatgcg ttaatgattt tgcatttggg tcaattaaat atttaacagc
     1681 gtttgcattt acatagtcaa ttaaactggc agccggatac acttctaaag atgtacctat
     1741 tacaataaac acatcggctg atgatgcaat aacagcggct gtttccatta acggcacagg
     1801 ctcgccaaac catacaatat gtggccgtaa ttgcgaccct aattcacatg tatcgccttc
     1861 ttttaattcc catccattta tgggataaat gagattgggc ttaacggtgc ttcgggcttt
     1921 aattatttcg ccatgtaggt gcaatacctt tttgcttccg gctcgttcgt gcaagtcatc
     1981 aatattttgt gttataacat gcacatcaaa agcatattgc agttcagcta tggcattatg
     2041 tgcaacattt ggcaaagctt ccagcacatt ttttctccgt tgattataaa aatcgagcac
     2101 cagtttctga ttacggaacc atgcttctgg tgtggcaacg tcttctattt tatgttgttc
     2161 ccataagccg ccactatctc taaaagtttt aataccactt tcggcactaa tacccgcacc
     2221 tgtaaatact acaatttttt tcatgcttaa cggtaaatca gtttgcagtt tgacatatta
     2281 aagccgcgta aaaattcggt tgtatccatt cgctttttac ctgccaattg tatgttggtt
     2341 aaacgcaaaa ttccaccaag gcaatatatg tttaagtatg ttttattatc ggtataaata
     2401 ataccgtcaa agtttacggt tgcagtttca taactacagc catatacttt catactgtat
     2461 aatttttgat catgtgttgt aaactcaaaa aaagctgccg gatatggcga caagcctctt
     2521 atatggttgt aaaccgcagt aacgggctta ttaaaattta ttaataaatg ttgtttaaac
     2581 agtttcggtg catcatgcaa tggttcaatg cttaattcat tttggttaat ggttttaacc
     2641 gtaccatttg caatagtttc taccgtttta ataaccaatg cgcccccagc ttccattagc
     2701 ttat
//
LOCUS       DAOMAA010000182         2696 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_32077_length_2696_cov_2.981825, whole genome shotgun sequence.
ACCESSION   DAOMAA010000182 DAOMAA010000000
VERSION     DAOMAA010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32077_length_2696_cov_2.981825"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1273)
                     /locus_tag="PLO59_03335"
     CDS             complement(<1..1273)
                     /locus_tag="PLO59_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00164.1"
                     /translation="MLHFLKLLKSYVALNVCLFISISVFSITAFAQPKLVTFTFYGNA
                     QGMLAENINAFVQDDKGFMWIGSSDGLLKFDGRTFKLYRKNDNKNAIPGNSVKSLVLL
                     NSKLWIATNEGLCTYQDDRFFTIPNLNKINFNALNTMAASADGFMYLAFDKDRLLKFD
                     TKSKQPVFSIISNKILTTYALYPLDKNHLITGHIGTSILCYVDNKKVATRLGYQKEQF
                     KFGYTITAIARDNKNQYWCGAWDNGLHCFNTNLTHKESFVFNNMPGYNSISDEITCLA
                     FINDSCLWFGTKNNGLFEFNTYSKTLHPIQAQHELPQRINTIYVDKLKRIWIGTPSGL
                     ALYDPHQTQIERFKLTTGQGKKLMANCFIRYNDTVLLIGTQSGIVVNTPTKQYLQPLT
                     HRAQNQAVFSMAFDFKNRLIVGTNQTILRMHS"
     gene            1378..2430
                     /locus_tag="PLO59_03340"
     CDS             1378..2430
                     /locus_tag="PLO59_03340"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HQV00165.1"
                     /translation="MLSLQSNMQPKPTCCIIRFYKVLISNVIMLAAVIDLGTNIFHLL
                     IVRLDKQQFVVEKTAKAIVKLGENGITTNKLHNDAIQRGLHAIIEFKQICNEFNVSKI
                     YAFGTAALRRAANANEFMQQVINLTGISIEIIDGDTEATFIWHGVKNAVKIENQIALI
                     MDIGGGSTEFILADNNQIYWKKSFDIGAALLLEKFKPSDPITLEEITAINNFLAEEIA
                     AVIVMCNQYKPKMLIGSSGSFDTFNDILQAQNNTSDNSNFNFLEINAYYNLHALLLQS
                     TLSERLTMPGMIPIRADMIVMATLLTKLVLEKWPFSYLIRSAFALKEGIVYSSFFTTH
                     HQPNYPTQHGKNISNR"
     gene            2405..>2696
                     /locus_tag="PLO59_03345"
     CDS             2405..>2696
                     /locus_tag="PLO59_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010417074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQV00166.1"
                     /translation="MAKILVIDDERAIRHALKEILEYEKHEVTEAADGIEALKIIEKE
                     KFEIVFCDIKMPKIDGMELLEKFQEKMPDVPMVMISGHGNIETAVEAIKKGAF"
BASE COUNT          890 a          449 c          497 g          860 t
ORIGIN      
        1 acgagtgcat gcgcaaaata gtttgattgg tgcctacaat cagtcggttt ttaaagtcga
       61 aagccatgct gaataccgct tggttttgtg cacgatgagt aagtggttgc agatattgtt
      121 tggtaggtgt atttactaca atgcctgatt gtgtaccaat tagcagcacc gtatcattgt
      181 atcggataaa acaattggcc attaattttt tgccttggcc agtagtcagc ttaaaccttt
      241 caatttgggt ttgatgcggg tcgtataatg ccaggcccga aggtgtgcct atccaaattc
      301 gtttcaattt atcaacgtaa atggtattaa tacgttgtgg taattcatgt tgggcttgta
      361 tgggatgcag tgttttacta taggtgttaa attcgaaaag gccattgttt ttagtaccaa
      421 accaaaggca actgtcatta ataaaagcaa ggcaagtaat ctcatcgctt atcgaattat
      481 agcccggcat gttgttaaaa acaaacgatt ccttatgcgt taaatttgta ttgaaacaat
      541 gcaagccatt atcccatgca ccacaccaat attggttttt attatcgcgt gcaattgctg
      601 ttatggtgta gccgaattta aattgctcct tttggtagcc aaggcgtgta gctacttttt
      661 tattatctac ataacataaa attgatgtgc caatgtgccc ggttattaaa tgatttttat
      721 ccaacggata taatgcatac gtagttaaaa ttttattgct tataattgaa aatacaggtt
      781 gtttggattt agtatcaaat tttaaaagcc tgtctttatc aaaagccaaa tacataaaac
      841 catcagccga tgccgccata gtgtttaatg cattgaagtt tattttattt aaattgggaa
      901 tggtaaaaaa acgatcatct tgataggtgc acagcccttc atttgttgca atccaaagtt
      961 tgctgtttaa taaaaccagt gattttacac tgttaccggg tattgcattt ttgttgtcat
     1021 ttttcctata aagtttaaaa gttcggccat caaatttgag caacccatca ctgctgccaa
     1081 tccacataaa acctttatca tcctgcacaa atgcatttat gttttcggca agcatacctt
     1141 gcgcattacc ataaaatgtg aatgtaacta atttgggttg tgcaaatgca gtaatactaa
     1201 aaacactaat gcttatgaat aaacaaacat ttaaagcaac atagctttta agcaatttta
     1261 aaaaatgcaa catcgcacaa tatacaatgc ttccttaatt tgatggattt atacaaggca
     1321 tattttacca ataaattaaa agtatttgat tattcgaaaa cctgaattta gttttatatg
     1381 ctttcattgc aaagcaacat gcaacccaag ccaacttgtt gcattatcag gttttacaaa
     1441 gttctaattt cgaatgttat catgcttgca gcggttattg atttaggtac caatattttt
     1501 cacctgctta ttgtaaggtt agataagcag caatttgttg tggaaaaaac agcaaaagcc
     1561 attgttaagt tgggcgaaaa tggaatcaca acaaataaat tgcataacga tgccatacaa
     1621 cgcggcctac atgccattat tgaatttaaa caaatttgca acgagtttaa tgtttcaaaa
     1681 atttacgctt ttggtacggc tgctttacgc agggctgcca atgccaatga atttatgcag
     1741 caagtaatta atcttaccgg catatcaata gaaattattg atggcgacac agaagcaaca
     1801 tttatttggc atggggttaa aaatgcagta aaaatcgaaa atcaaattgc gctgataatg
     1861 gacattggtg gtggcagtac agagtttatt ttggccgata acaatcaaat ttattggaaa
     1921 aaaagctttg atataggtgc tgctttattg ctcgaaaagt ttaaaccatc agacccaata
     1981 acattggaag agataacagc aataaataac tttttagccg aagaaattgc agcagtaatt
     2041 gtaatgtgca accaatataa acccaaaatg ctcattggct cctcaggttc attcgatacg
     2101 tttaatgata tactgcaggc acaaaacaac acttctgata atagcaattt caattttctt
     2161 gaaattaatg cgtattataa tttgcatgca ttattattgc aaagtaccct ttccgaacgg
     2221 ttaacaatgc ccggtatgat acccatcaga gcagatatga ttgtgatggc aacattattg
     2281 actaaattgg ttttggaaaa atggcctttt agttatttaa ttcgctccgc ctttgcatta
     2341 aaagaaggca ttgtttatag cagttttttt acaacacacc accaacccaa ttacccaaca
     2401 caacatggca aaaatattag taatagatga tgaaagagct atcaggcatg cgctaaaaga
     2461 gattttagaa tatgaaaaac atgaagtaac cgaagctgct gatggcattg aagctttaaa
     2521 gattattgaa aaggaaaagt ttgaaattgt tttttgcgat atcaaaatgc ctaagataga
     2581 tggcatggaa ttattggaga agtttcagga aaaaatgcct gatgtgccaa tggttatgat
     2641 atcgggtcat ggtaatatcg aaactgcagt tgaggcaatt aaaaagggtg ctttcg
//
LOCUS       DAOMAA010000183         2688 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_32269_length_2688_cov_2.747057, whole genome shotgun sequence.
ACCESSION   DAOMAA010000183 DAOMAA010000000
VERSION     DAOMAA010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32269_length_2688_cov_2.747057"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2114
                     /locus_tag="PLO59_03350"
     CDS             <1..2114
                     /locus_tag="PLO59_03350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HQV00167.1"
                     /translation="PDIHCSSCIWLLENFKRVDAGVIQSKVNFIRKEVFIIFDESKTS
                     LRKIVESLARVGYEPSLHLDSISKKEKQKTNRNRIIRIGVAGFCFGNIMMLSFPEYFS
                     SGNLADPGLKNFFSYLTFLLALPVFFYSASEFFIKSYQALKQKSTSIDVPIAIGIAAI
                     FIRSSYEIIAHTGPGFFDSGSGLIFFMLIGRWFQDFTFDALAFERDYKSYFPIAVNTY
                     KNGIEKPVPVSDLKVNDKIRVRNNELIPVDATLIKGQAFIDYSFVTGESLPVRVDAGQ
                     IIYAGGRQTGSIIELLVVKEISQSHLTQLWNNDVTKSFESKFEKLVNAISRYFVLVTL
                     LIATVAAAYWWQVDIKKAINAFTAVLVIACPCALALSAPFTYGNIMRILGKQKIFVRN
                     YQTLENLADINTIVFDKTGTLTENNTAEISFVGAKLTDHEVQLIYALVRHSSHPLSKL
                     LSQHFKLNDPQHLTDYTEVPGQGISGYVNQINIKAGSEIFVTGNKLANDNLTKVYIAI
                     NHVCKGYFVFINTYRSGLKHLAENLVHEKFHLSVLTGDGNAEQHNLATIFSNKVELHF
                     NCTPEQKLNSIKSLQQQNKKVLMVGDGLNDAGALRQSNVGISITDNTNNFTPASDVII
                     DASQLINLHKLIAYSQRASHIIIWSFIISLLYNFIGLSFAVTGTLSPLIAAILMPIST
                     ISLVLFTLVASTVKSRLLGFK"
     gene            2207..2404
                     /gene="ccoS"
                     /locus_tag="PLO59_03355"
     CDS             2207..2404
                     /gene="ccoS"
                     /locus_tag="PLO59_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HQV00168.1"
                     /translation="MSVIIILIAVSLLVAIGFLISFLWAVKTGQYDDDYTPSVRILFD
                     NEFKDESTSANEKNIHSNNNN"
     gene            2425..>2688
                     /locus_tag="PLO59_03360"
     CDS             2425..>2688
                     /locus_tag="PLO59_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003005370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HQV00169.1"
                     /translation="MTLEKFEYDNKIVRNFAIATMVWGIVAMLVGLLAALQLPFPAFN
                     FDSHATFGRVRPLHTNAVVFAFVGNAMFMGIYYSLQRLLKARMY"
BASE COUNT          846 a          503 c          499 g          840 t
ORIGIN      
        1 tacccgatat ccattgcagc agttgcattt ggttactcga aaattttaag cgcgttgatg
       61 caggtgttat tcaatctaag gttaatttta tacggaaaga agtatttata atttttgatg
      121 aatcaaaaac atcattaaga aaaattgttg agtcgcttgc ccgggttggc tacgagcctt
      181 cactccatct cgattcgatt agcaaaaagg aaaaacaaaa aacaaaccgc aacagaatta
      241 taaggatagg tgtagccgga ttttgttttg gcaatataat gatgcttagc tttcctgagt
      301 atttctcgtc cggaaacttg gccgacccag gcttaaaaaa ctttttcagc taccttactt
      361 ttctcttggc cctgcctgtt tttttctaca gcgcgtctga gttttttatt aaatcatatc
      421 aggcactaaa acaaaaatct acctctattg atgtgcctat tgccattgga attgccgcta
      481 tatttatacg cagcagttat gaaataatag cacacaccgg gcccggtttt ttcgacagtg
      541 gttcgggctt aatatttttt atgctgatag ggcgctggtt tcaagatttt acttttgatg
      601 cactggcttt cgaacgcgac tataaatcat atttccccat tgcagttaac acatataaaa
      661 atggtatcga gaaaccggta cccgttagcg atttaaaagt aaatgataaa atacgggtac
      721 gaaataatga actcattccg gttgatgcca ctttaataaa aggccaggct tttattgact
      781 atagttttgt aacaggcgaa tcgttaccgg tgcgtgtaga tgcaggccag ataatttatg
      841 ccggtgggcg tcaaacaggt tcgataattg agttgttggt agtaaaagag atttcgcaaa
      901 gccatctcac acagctttgg aataatgatg taactaaatc ttttgaaagt aaatttgaaa
      961 aactggtaaa tgctatcagt cgttactttg tattggtaac attgcttatt gcaaccgttg
     1021 cagcagccta ttggtggcaa gttgatataa aaaaagccat taatgctttt acggccgtac
     1081 ttgtaatagc ttgcccatgc gcattggcat tgtcagcgcc attcacttat ggaaatataa
     1141 tgcgcatact gggtaaacaa aaaatatttg tgcgcaatta tcaaacctta gaaaacctgg
     1201 ccgatataaa taccatagtt tttgacaaaa ccggaacgct taccgaaaat aatacagcag
     1261 aaatttcatt tgttggtgct aaacttacgg accatgaagt acaattgatt tatgcattgg
     1321 tgcgccattc atcgcatccg ttaagtaaat tattatcgca acattttaaa ctgaatgacc
     1381 cacagcatct tacagattac actgaggtac ccggccaagg tataagcggc tatgtaaacc
     1441 agataaacat taaagccggc agcgaaatat ttgtaaccgg aaataaactt gccaatgata
     1501 atcttacaaa ggtttatatt gctattaacc atgtgtgcaa gggctatttt gtttttataa
     1561 acacatacag atcaggctta aaacaccttg ccgaaaattt agtacatgaa aaatttcatt
     1621 tgtcggtttt aacaggcgat ggcaatgcgg agcaacacaa cttagcaacc attttttcaa
     1681 ataaagttga attacacttt aattgcacac cagagcaaaa gttgaactct ataaaatcgt
     1741 tacaacaaca aaataaaaaa gtactgatgg taggcgatgg ccttaacgat gccggagctt
     1801 tgcgacaaag taatgtaggt atttcaataa ccgataatac aaacaacttt actccggcaa
     1861 gtgatgtaat aattgatgca tcgcagctta taaaccttca taagctcata gcgtattcgc
     1921 agcgtgcatc acacatcatt atttggagtt tcatcatatc gttgttatat aattttatcg
     1981 ggttgtcatt tgccgtaacc ggcactttat cgccattaat tgcagctatc ttaatgccca
     2041 taagtacaat aagtttagtc ttatttactt tagtggcaag cactgtaaaa agtaggttat
     2101 taggttttaa ataggtttct tgattataaa atatgacata agtcatattc cacagtaata
     2161 gttctcataa aacacaattc ttttacaaaa tagctttgcc acccttatga gcgttatcat
     2221 tattttaata gcggttagct tacttgtagc aatcggtttt cttatttcat ttttatgggc
     2281 tgtaaaaacc ggtcagtacg atgacgatta tacaccatcg gtacgcattt tatttgacaa
     2341 tgaatttaag gatgaatcaa catctgcaaa cgaaaaaaat attcactcaa acaataataa
     2401 ttaaacctcg aactgtttca ctgtatgaca ttggaaaaat ttgaatacga caataagata
     2461 gtccgcaact ttgccatagc aactatggta tggggcattg ttgccatgct ggtaggctta
     2521 cttgcagcct tacaactgcc ttttccggca tttaacttcg actcgcatgc cacatttggc
     2581 agagttaggc ccttgcatac caatgcagta gtttttgcct ttgtgggcaa tgccatgttt
     2641 atgggcattt actattcgct acaacgtttg ttaaaagcgc gcatgtat
//
LOCUS       DAOMAA010000184         2687 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_32286_length_2687_cov_2.917553, whole genome shotgun sequence.
ACCESSION   DAOMAA010000184 DAOMAA010000000
VERSION     DAOMAA010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32286_length_2687_cov_2.917553"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="PLO59_03365"
     CDS             complement(<1..752)
                     /locus_tag="PLO59_03365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HQV00170.1"
                     /translation="MQLKKIKPQPIKTKSGLAKNIEAPKWAHPLSNSLALFVGFIGCI
                     IYAQTLGFGFALDDYSAIIENRQTKQGIAALGEIFKSSYRFGYNIQGDELYRPLSKAA
                     FAVLWQWAPNNAFPFHLLNLVMYALTGYLLFITLQRFTANIYVSFIASLLFITHPIHV
                     EVVANIKSLDEILGFLFFIIALNLLHRNIQKPQTSTVLLSCLSFFLALLSKESSITFV
                     LVFPLFVYFFTNHTVTQSLSKTWVFWIPAILF"
     gene            complement(773..1303)
                     /locus_tag="PLO59_03370"
     CDS             complement(773..1303)
                     /locus_tag="PLO59_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00171.1"
                     /translation="MIFNKVRLFAISVGIIIVAGCNENSKLQKEIAQKESELKNDTTG
                     VVNYAKAQQMIDLYISYARLNPVDTISAYYLFKAADVAAHAHQPQLALNLYDSVIINF
                     PKYNGVADAYFFKAFLLDNVQYDKVKASEAYQQLLVKFPNYHQAESVRWLLQNMYKTD
                     LEIAHELIGKQNDSIQ"
     gene            complement(1343..2140)
                     /locus_tag="PLO59_03375"
     CDS             complement(1343..2140)
                     /locus_tag="PLO59_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQV00172.1"
                     /translation="MQVVIITGATSGIGKALAIQYATQNFTVVIAGRNKNVLLETETE
                     INKFGGRSKAFVCDVTIEADCKNLIQETLKLYSRIDILICNAGISMRALFIDVDLDVL
                     QKLMNTNFWGAVYCTKYALPSLLINKGSVVAISSIAGKQGLPARTGYSASKFALEGFM
                     ETLRAENLKTGLHVLIARPGFTASNIRYAALNATGKMHNQSSRDESKMMPAEIVAKHI
                     IAAVHKRKRSITLTLQGKLAVFLSKFFPRLLDNIVYNEMRKEKNAPL"
     gene            2326..>2687
                     /locus_tag="PLO59_03380"
     CDS             2326..>2687
                     /locus_tag="PLO59_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00173.1"
                     /translation="MIVSCIGLFFGGSHISKLVHLYVEALHAQNVTAVVKSKILSEKS
                     EAFKTAYVLNFNITNLNQLYNGKIFYSLNDKLNTGDSVAIIHCSQIKYSTFSGFSNRY
                     ASSLFYAVIFLILGSAFG"
BASE COUNT          827 a          451 c          481 g          928 t
ORIGIN      
        1 aaaaacaaaa tagcaggtat ccaaaaaacc caggttttgc ttaacgattg tgtaacagtg
       61 tggtttgtaa agaagtaaac aaacaatgga aacaccagaa caaaagttat agacgactct
      121 ttgcttagca aagccaaaaa gaaacttaag caggaaagta gtactgtact tgtttgtggt
      181 ttttgaatgt tgcgatgcag taaatttaat gcgatgataa aaaacagaaa gcctaaaatt
      241 tcgtctaaac ttttaatgtt ggcaactact tcaacgtgta tggggtgggt tataaataat
      301 aaactcgcaa taaacgatac gtatatattg gctgtaaatc gttgtagcgt aataaataat
      361 aaatatccgg ttaaggcata cattactaaa ttaagtaaat ggaaaggaaa tgcattgttt
      421 ggagcccatt gccatagtac agcaaaagca gccttgctta atgggcgata aagttcatca
      481 ccttgaatat tgtagccgaa acggtaactg cttttaaaaa tttcgcctaa agcagctata
      541 ccttgtttgg tttgtcggtt ttcgattatt gcgctgtaat cgtctaaggc aaatccaaaa
      601 cctaatgttt gtgcataaat gatgcatcca ataaaaccga caaataatgc cagcgaatta
      661 gaaagtggat gcgcccactt gggtgcttct atattttttg ccaaccctga ttttgttttt
      721 atgggttgag gcttaatttt tttaagttgc atagtagggt ggtatgttgg ttttactgta
      781 tggagtcgtt ctgctttcct attaattcgt gtgcaatttc taaatcagtt ttatacatgt
      841 tttgtaataa ccaccttacg gattctgcct gatgatagtt tggaaacttt accagcagtt
      901 gttggtaggc ttcgctggct tttactttat cgtattgcac attgtcaagc aagaaggctt
      961 taaaaaaata agcatcagct accccattat actttgggaa attaataatc acactgtcat
     1021 aaaggtttaa ggccaactgt ggctgatgtg catgggcagc aacatctgca gctttaaata
     1081 agtaataagc acttattgta tcaaccgggt tgagccgggc atagctgatg tataggtcaa
     1141 tcatttgttg ggctttcgca taattaacta cacctgtggt gtcatttttt aactccgact
     1201 ctttttgagc tatttctttt tgtaatttac tattttcatt acaacctgct actatgatta
     1261 taccaacact aattgcaaat aagcgaactt tattaaaaat catatattaa agtttaaagt
     1321 gaaaattttt ttgcagttaa atttaaagcg gtgcattttt ttcttttctc atttcgttgt
     1381 aaacaatatt gtctaacaag cgaggaaaaa acttacttaa aaatacggcc agtttgcctt
     1441 gcagcgttag tgtaatacta cgcttacgtt tatgtactgc agctattata tgtttggcta
     1501 ctatttcagc aggcatcatt ttactttcgt cacgcgatga ttgattgtgc attttgcccg
     1561 tggcatttaa tgcagcatac cttatgttac tggctgtaaa accgggtctt gctatcaata
     1621 catgcaaacc tgtttttaag ttttcggccc gaagcgtttc cataaatcct tctaatgcaa
     1681 atttagatgc actgtaaccg gtgcgagccg gtaatccttg ttttccggca attgatgaaa
     1741 tggccacaac gctacctttg tttattaaaa gcgatggtaa agcatatttg gtacaatata
     1801 cagcacccca aaaattagtg ttcattaact tttgtaaaac atctaaatca acatctataa
     1861 acaatgcacg catactaatg ccagcgttac atattagtat atctattctg ctatataatt
     1921 ttaatgtttc ttgaatcaga tttttacagt cagcctctat ggttacatca catacaaatg
     1981 cttttgagcg tccaccaaat ttatttattt ctgtttcagt ttcaagaagc acatttttat
     2041 tacgccctgc aattactacg gtaaagtttt gtgtggcata ctgtattgcc aaggctttgc
     2101 ctattcctga tgtggctcct gttattatta ctacttgcat ataatctttc agcataaagc
     2161 gcaacaaagg aaagatattt ttaggcagtg tatcatagcg acttttgatt tattctgaaa
     2221 cttagtttgt aaaaaaatta atttcagttg atttttaaac aaaatataaa atgtaaatat
     2281 gcttttaggt atatgaaaag tgcaaatcca atacatttaa tttggttgat tgtttcatgc
     2341 ataggtctct tttttggtgg tagtcatatt tccaagctgg ttcatttgta tgttgaagcc
     2401 ttacatgcac aaaatgttac ggcagttgtt aagtcaaaaa tactttccga aaaaagtgaa
     2461 gcgtttaaaa cggcttacgt acttaatttt aatattacaa acctcaatca gttatataat
     2521 ggtaagatat tttattcgct caacgacaag ctaaacacag gcgattcggt agctataata
     2581 cattgtagtc aaataaagta tagtacattt agtggatttt cgaaccgata tgcaagctca
     2641 cttttttatg cagtgatttt cttgattctg ggttcagctt ttggttt
//
LOCUS       DAOMAA010000185         2686 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_32310_length_2686_cov_2.783732, whole genome shotgun sequence.
ACCESSION   DAOMAA010000185 DAOMAA010000000
VERSION     DAOMAA010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32310_length_2686_cov_2.783732"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1618)
                     /locus_tag="PLO59_03385"
     CDS             complement(<1..1618)
                     /locus_tag="PLO59_03385"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HQV00174.1"
                     /translation="MMYSKKTLRNNFTAPVFLLVAISVCCTTMFCNVKTNVPAQSVTT
                     YAGLNDTVQYVGMQTCKQCHAAIYNTFIETGMGQSFDVASKQKSSADFAHATIYDKHS
                     DLYYHAAWQNNYLMFSEYRLQQHDTIHAHKQLINYIVGSGQHTNSHIYNNNGYLYQAP
                     MTYYTQKGIWDLPPSFENGNNTRFSRAIGQECITCHNAYPTMQMGSENKYSFVANGID
                     CERCHGPGAAHVQHIQAGHLTDTANAIDYSIVNPAKLPINLQLDVCQRCHIQGNAVLK
                     PGKSFFDFKPGISLSSVMDVYMPVYTGQETEHIMASHAERLKMSQCYINTSEQISNNT
                     AKLQALRPYKNALTCITCHNPHVSVKVTGKEIYNRACVNCHQSNGHKLCTAPTVKLNA
                     AKNNCIQCHMPKSGAIDIPHVRVTDHYIAKPVPAKEVQKIKQFIGLACINNNHADSLS
                     MAMAYINYYEKFTNETFALDSAARYLPDNNVNPNNFNQLIHLAYLRSDYQKVLSYNRM
                     VGNSLSILNQQSYTNTHAWTCYRISESFWQL"
     gene            1990..>2686
                     /locus_tag="PLO59_03390"
     CDS             1990..>2686
                     /locus_tag="PLO59_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00175.1"
                     /translation="MKKIILSLIAFIYMFALNSKAQFAPATIVANNAQPSYGPFILTN
                     CDMLTGLNTLSNTRISVIVMTDGVAGTLFSEAVNATTGNLLGTNIINLADTDATFADV
                     CLVARANSVYALVLYKKASNGHTVLQTFLWHAPTYSFTDVQTQTLDTDPMDEMGHIDS
                     DTNDYWVATWSTVGTQTIKTCVGRTNSTGPFIVNNCSNVELDYPNNIGAIGDLRNPDV
                     AIYSNSNVTPSVTT"
BASE COUNT          778 a          517 c          522 g          869 t
ORIGIN      
        1 gtaactgcca aaaactttcg ctaatgcggt aacaggtcca ggcatgtgtg ttggtatatg
       61 attgctgatt taatattgac aggctattgc caaccatacg gttatagcta agtacttttt
      121 gataatcgct acgcaaataa gccaaatgaa ttaactggtt aaaattattt gggtttacat
      181 tattatcagg caaataacgg gcagccgagt cgagtgcaaa tgtttcattc gtaaattttt
      241 cgtagtagtt gatgtaggcc attgccatag ataacgaatc ggcatggttg ttatttatgc
      301 aagccaaacc tatgaactgt tttatttttt gtacttcttt tgccggcacc ggtttggcta
      361 tgtaatggtc ggtaacacgt acgtgtggaa tatcaatagc gccactttta ggcatgtggc
      421 attgtatgca attgtttttg gccgcattta gttttaccgt tggtgctgta caaagtttgt
      481 ggccattact ttgatggcaa tttacacagg cgcggttata aatttctttg ccggttactt
      541 ttacacttac atgcggatta tgacatgtaa tacaagtaag tgcatttttg taaggacgta
      601 aggcttgtaa ctttgccgtg ttgttactta tttgctctga tgtattaata taacattgac
      661 tcatttttaa acgctcggca tggcttgcca taatatgttc cgtttcctga cctgtataaa
      721 cgggcatgta aacatccatt acactgctta acgatatacc gggtttaaaa tcgaaaaaac
      781 ttttgccggg ttttaatacg gcatttcctt gtatatggca acgctggcac acatcaagct
      841 gcaagttaat aggcaactta gccgggttta caatactgta atcaattgca tttgctgtat
      901 cggttaagtg ccccgcttgt atgtgttgca catgtgcagc acccggcccg tggcaacgtt
      961 cgcagtcaat gccattagct acaaaactgt atttgttttc gctacccatt tgcatggttg
     1021 ggtatgcatt gtggcaagta atacattcct ggccaatggc gcggctaaag cgcgtgttat
     1081 taccattttc gaaactgggt ggcaaatccc atattccttt ttgtgtatag taagtcatgg
     1141 gcgcctgata caggtaacca ttgttattat aaatgtgcga attggtatgt tgcccgctgc
     1201 caactatata atttataagt tgtttatgcg catgtatggt atcgtgttgc tgcaaccgat
     1261 actcgctaaa cattaagtaa ttgttttgcc atgctgcatg atagtacaaa tcgctatgct
     1321 tatcataaat ggtagcatgg gcaaaatctg ctgaggattt ttgttttgaa gctacatcaa
     1381 acgactgccc cataccggtt tcgataaatg tattatatat agctgcatgg cattgtttgc
     1441 atgtttgcat gcctacgtat tgcaccgtat cgtttaagcc ggcataggta gtaacacttt
     1501 gagcaggcac attagttttt acgttgcaaa acatggtagt acaacaaacg ctgattgcca
     1561 ctaataaaaa aaccggagca gtaaagttgt tacgaagcgt tttttttgag tacatcatct
     1621 atgcagcaaa tgtatggctt gcatgatgca aagcacaaaa aaacttctat tgattaaaga
     1681 tgccatatac gtatcatttt aattttatat catttttatg cgctaactat ttgatttagc
     1741 aacttttcgg tttttaataa aactaaagtt gaattaaaat aattatttaa tttggtaggt
     1801 acgaaattta acctatgcta cacgctacac gctacacgct acacgctaca cgctacacgc
     1861 tacacgctac acgctaagcc tatgcgtagc tgtaaattac aaaacccatt actattgtaa
     1921 acttcgttat ggcgatgtaa gttaaacgat tacacaaact ttaatcttta acttataatc
     1981 cataataaaa tgaaaaaaat aattttatcc ttaatagcct ttatctatat gtttgcttta
     2041 aacagcaaag cccagtttgc ccctgctacc attgttgcca acaatgcaca gccttcttat
     2101 ggaccattta tacttaccaa ttgcgatatg ctaacaggct taaacacttt aagtaacaca
     2161 cgtatttctg ttatagtgat gactgatggg gtagctggta cattatttag tgaagctgtt
     2221 aatgcaacaa ccggtaatct acttggcaca aatattatca atcttgctga taccgatgca
     2281 acatttgccg atgtatgttt ggttgcccgt gctaacagtg tgtatgcctt agtactttat
     2341 aaaaaagcca gtaacggaca taccgtttta caaacttttt tgtggcatgc gcctacatat
     2401 tcatttacag atgtacaaac acaaacactt gataccgacc ctatggatga aatggggcat
     2461 attgatagcg acacaaacga ttattgggtg gctacctgga gtaccgttgg cacccaaacc
     2521 attaaaacgt gtgtaggccg caccaatagc accgggccgt tcattgtaaa taattgtagt
     2581 aatgtagaat tagattatcc aaataatata ggcgcaattg gtgatttacg caaccctgat
     2641 gtagccattt atagcaactc aaacgttaca ccatcggtta ccacaa
//
LOCUS       DAOMAA010000186         2685 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_32322_length_2685_cov_4.036122, whole genome shotgun sequence.
ACCESSION   DAOMAA010000186 DAOMAA010000000
VERSION     DAOMAA010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32322_length_2685_cov_4.036122"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="PLO59_03395"
     CDS             <1..698
                     /locus_tag="PLO59_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00176.1"
                     /translation="NPIIFLRPVEFSMSSADNVLLGLNTKFKINSKNILYGQLSLDEF
                     NLTALKNKDGDYRNKFGFQLGYKSYHVFGIKNLRFQTEFNYVKPYTFQHRSSLTNYGH
                     YNEALAHVQGANFYESVTFINYQFKNWFFQLQGMYVATGLDTAGINFGKNIFDTYTQP
                     AQFTGNKMLQGLQSTITYFDFKIAYLINPKYNLRFECGATSRTLDNDLFTQKSTFVYA
                     GIRTTISNQYFDF"
     gene            876..1943
                     /locus_tag="PLO59_03400"
     CDS             876..1943
                     /locus_tag="PLO59_03400"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HQV00177.1"
                     /translation="MIEITFASIVAFAITFWAIPAIIKVAYIKHLFDQPDERKVHQNV
                     TPRLGGVAIFGGTLFAIACFTVGFNNHTIQYIIAALLIIFFIGLKDDLVTLTPVKKFA
                     GQFLAALIITCIADIRITSLCGIFGIYALPYWASVLFSIVTIIGITNSFNLIDGIDGL
                     AGGIGAIAAFTFGFWFYNYKELAMCTVAFSLFGSLLGFLVYNFSPAKIFMGDTGSLFT
                     GMLLSILTIYFIQLSFYATPYSFPFRSSPAMAISILIIPLFDTIRIFLWRLSKKRSPF
                     QPDRNHMHHILLDIGCSHRQATLILYIVNILFVVLAVSLRNISSLVLLVIIIVLAIIL
                     SIIPIAIRNVMRNKALSAKTA"
     gene            2003..>2685
                     /locus_tag="PLO59_03405"
     CDS             2003..>2685
                     /locus_tag="PLO59_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HQV00178.1"
                     /translation="MAKQKAAAINLAFNHGWTYAPAPESTDHIEIKSQYDLFINGKFV
                     APTQGQYMDSINPANEKKLSSVAIAQTADVDKAVKAAQQASTGWQKLKPSERGKYLFR
                     IARMLQERGRELAIIESLDGGKPIRESRDVDIPLAAAHFFYYAGWADKLAYAFPGRDA
                     QPLGVAGQIIPWNFPLLMAAWKIAPALACGNTVVLKPAETTSLTALKLAEIIQACELP
                     PGVVNIITGA"
BASE COUNT          775 a          538 c          481 g          891 t
ORIGIN      
        1 taaatccgat tatttttttg cgtcccgttg aattttcaat gtcttcggcc gataatgttt
       61 tattaggcct taataccaag tttaaaatta actcaaaaaa tattttatac ggtcagcttt
      121 cattagatga atttaacctg actgcattaa aaaataagga tggcgattac cgcaacaaat
      181 ttggttttca attaggttat aaaagttacc atgtatttgg cataaaaaat ttacgctttc
      241 aaacggagtt caactatgta aagccataca catttcagca tagaagctcg ctcaccaatt
      301 acggccatta caacgaagct ttggcccatg tacaaggagc taatttttac gaaagtgtaa
      361 cctttattaa ctatcagttt aaaaactggt tttttcaatt acaaggtatg tatgttgcca
      421 ccggattaga tacagccggt ataaactttg gcaaaaatat atttgatacg tacacccaac
      481 ctgcacaatt tacaggcaac aaaatgttac aaggcctgca aagcacaata acttattttg
      541 attttaaaat tgcttactta ataaatccga aatacaacct gcggttcgag tgtggtgcaa
      601 catcacgcac acttgataac gatttattta cacaaaaaag cacttttgtt tatgccggca
      661 ttcgtactac aatcagtaat caatactttg atttttaata ctataaaaat gcaaccttta
      721 aaaccgtttt catcaacatc cattacttaa taagttcata ttaaacggat attaatttat
      781 caacctgttt cgacatttta cggttttaaa aaatattgtt cctttacgat aattaacaat
      841 gacaaaacgg tgtttcttaa tcaaacaagt ctgctatgat tgaaattaca tttgcctcca
      901 tagtggcttt tgctattaca ttttgggcca tacctgccat aattaaagta gcctacatta
      961 aacatttatt cgaccagcct gacgagcgaa aggtgcatca aaatgtaaca ccccgtttgg
     1021 gtggtgtggc catttttggc ggcaccctgt ttgccattgc ttgttttacc gttggtttta
     1081 ataatcacac catacagtac attattgccg cattgctcat tatttttttt attggcctta
     1141 aagacgattt agttacccta acaccggtta aaaaatttgc agggcagttt ttggccgcac
     1201 ttattattac ctgtattgcc gatatacgta ttaccagcct ttgcggtatt tttggtattt
     1261 atgctttgcc ttattgggcc agcgttttat tttctattgt aactataatt ggtattacca
     1321 attcgtttaa cctcattgat ggtattgatg gtttagccgg aggcattggt gctattgccg
     1381 cttttacttt tggcttttgg ttttataact ataaggaact tgctatgtgc actgttgctt
     1441 tttcactgtt tggatccctg ttgggttttt tagtttataa tttttcaccg gctaaaattt
     1501 ttatgggcga tacaggctcc ctgtttacag gtatgctgct ttctatactt accatttatt
     1561 ttattcagct tagtttttac gccacaccgt attcatttcc attcaggtcg agcccggcta
     1621 tggcaatcag cattttaatt attccattat tcgatacgat tcgtattttt ttatggcggt
     1681 tatcaaaaaa gcgttctccg tttcagcccg accgtaatca catgcaccac atattgctag
     1741 atattggttg tagtcatcgg caagctacgc ttatacttta tatcgttaat atactgtttg
     1801 ttgtattagc tgtttcgtta cgtaacatca gtagtttggt tttacttgta ataataattg
     1861 tattagccat tatactaagt attattccaa ttgcaatacg caatgtcatg cgcaataaag
     1921 cactcagtgc aaaaactgcc tgaacaatgg tgtctttaaa cttacttccc attacatatt
     1981 aattttttta tccggttaaa atatggcaaa gcaaaaagca gcagcaatca acttagcatt
     2041 taaccacggg tggacttatg cgcctgcacc cgaatcaacc gaccatattg aaattaaatc
     2101 gcaatacgat ttatttatta acggaaagtt tgtagcccca acgcaaggcc aatacatgga
     2161 tagtataaat ccggcaaacg aaaaaaaact atccagcgtt gccatagcgc aaacggccga
     2221 tgttgacaaa gcggtaaagg cagcgcaaca agccagcacc ggttggcaaa aactgaaacc
     2281 atccgaacgc ggcaaatatt tatttcgtat agcacggatg ttacaagaac gtggccgcga
     2341 gttagctatt atcgaaagct tggatggcgg caaacccata cgcgaatcgc gtgatgttga
     2401 tattccgttg gctgcggctc acttttttta ttatgcagga tgggctgata aattagctta
     2461 tgcatttccg ggtcgcgatg cccaaccttt gggtgtggcc ggtcagataa ttccctggaa
     2521 ttttccattg cttatggctg cctggaaaat tgccccggca cttgcatgcg gcaataccgt
     2581 tgtacttaag ccggccgaaa caacttcgct tacggcttta aaacttgccg aaattataca
     2641 agcatgcgag ttgccacccg gtgtggttaa tataattacc ggtgc
//
LOCUS       DAOMAA010000187         2680 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_32448_length_2680_cov_2.789333, whole genome shotgun sequence.
ACCESSION   DAOMAA010000187 DAOMAA010000000
VERSION     DAOMAA010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32448_length_2680_cov_2.789333"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="PLO59_03410"
     CDS             complement(<1..432)
                     /locus_tag="PLO59_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00179.1"
                     /translation="MKKVILLCVSIALCPKMIFSQSTRTPNTWGLTTDYLGWDATAGS
                     PQLNIRTTNSDAINFYTNFGSFASTRMRIMGGNSATGGFVGIGYNHTPNFQLDVLNDI
                     NIQVNAINNAYRLNGVTVLQNWGARNIFTGAGAGAALTLGAD"
     gene            complement(437..1381)
                     /locus_tag="PLO59_03415"
     CDS             complement(437..1381)
                     /locus_tag="PLO59_03415"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV00180.1"
                     /translation="MKKLVLLVSALVFHSLIFAQINLVPNPSFEDTVSCPTTANQIDK
                     AVGWHASRESPDYFNECDFITGNTSVPGNFTGFQYARSGVAYAGFIAFYYPFQNGREF
                     FYSQLNSPLQIGHKYVVSLYVSWSAGVSDLIACNKLGANFSMTSYSSSINPQPILNYS
                     HIYTDSIVQDSSGWFKIQGSFVADSNYLFLSIGNFFDDFNTDTIMPGFPSADAYYYVD
                     DVSVVEDTTSGFDNNDNLNEIIISPNPCYNSVYLKNINKFSMVYLYAMDGKLIDQYII
                     PKNKNEIQIDLTPYSKNMYVLKFTNGKKEFSYSKLFKL"
     gene            complement(1567..>2680)
                     /locus_tag="PLO59_03420"
     CDS             complement(1567..>2680)
                     /locus_tag="PLO59_03420"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HQV00181.1"
                     /translation="IRFALLICMSIYACIYFIIYSNTKVFYNYMYAVLIIWFVFFLCI
                     LQSFTGLIILSVTLGIYVICFLIHQKRWMYTATIFLAIITVLIGLGIKIKNDLDNLNT
                     TKQTTQPLPVYTALGNLYTHERFNGDLENGYPIFVNVAYSELDSAWQTRSSIDFDSVD
                     ARGQWIKYTLLRYMTSKGLLKDANGLMQLSETDIKNIEAGIASVNQLQGNSISARYHA
                     TLYELNNAWQGGNPSGHSLTMRLLFWKASLHIISNHLLFGVGTGDNKLELDKYYASNT
                     PQLFKQFWLRSHNQFLAIAVSFGLTGLLIFITMLLWPATKLNRWSQFLYVTFFITAIC
                     SFLTEDTLETQAGVTFFAFFNTFFLFQNQTPEINRT"
BASE COUNT          891 a          479 c          479 g          831 t
ORIGIN      
        1 atcggcacct aaggtaagtg ctgcaccggc acctgcaccg gtaaatatgt tgcgggcgcc
       61 ccaattttgc aatactgtaa ctccgttaag gcggtatgcg ttgttgatgg cgtttacttg
      121 tatgtttata tcgttgagta cgtctaattg gaaattgggt gtgtgattat aaccaattcc
      181 aacaaacccg cccgtggcgg aattgcctcc cattatgcgc atacgtgtcg aggcaaaact
      241 gccaaagtta gtgtagaagt ttatagcatc agagttggtg gttctgatgt ttaattgcgg
      301 actgcctgca gtggcatccc agccgagata gtcagtagtt aaaccccaag tatttggtgt
      361 tctcgtggat tgactaaaaa tcattttagg acataatgcg atggatacgc acaataaaat
      421 tacttttttc atgatgttat aatttaaaca gtttagaata tgaaaattct tttttgccat
      481 ttgtaaattt taatacatac atatttttag aatatggtgt taggtctatt tgaatctcat
      541 ttttattttt agggataata tattgatcaa ttaatttccc atccattgcg tacagataaa
      601 ccatgctgaa tttattaata ttttttaagt aaacactatt gtaacaagga ttaggtgata
      661 ttatgatctc attcaaatta tcattgttat caaaacctga tgttgtgtct tctacaacac
      721 taacatcatc tacataatag tatgcatccg cagatgggaa acctggcatt atagtatccg
      781 tattaaagtc atcaaaaaaa ttaccaattg ataaaaacaa atagtttgag tctgcaacga
      841 aacttccctg tattttaaac caccctgatg aatcttggac aatcgaatca gtataaatgt
      901 gcgagtaatt taaaattggt tgagggttaa ttgatgacga atagcttgtc atactaaaat
      961 tagctcctaa tttattgcac gcaattaaat cgcttactcc cgctgaccaa ctaacataaa
     1021 gtgaaacaac atatttatgt ccaatttgta aaggggaatt taattggcta taaaaaaatt
     1081 cacgcccatt ttgaaatggg tagtaaaacg ctataaatcc tgcataagca acaccactac
     1141 gtgcatattg aaatccagta aagtttcccg gcactgaagt gttacccgta ataaaatcac
     1201 actcattaaa ataatcagga gattcgcgac ttgcatgcca gccaacggct ttgtctattt
     1261 ggttggctgt ggttggacaa ctcactgtat cctcaaaact cgggttaggt actaaattta
     1321 tttgtgcaaa tattaaactg tgaaatacca gagcagatac taaaaggact aattttttca
     1381 ttactgcaat tatttatcaa taaaagtaaa aaaaaggtct gaactgctaa tttgttagct
     1441 atagctgatt gctgcattgg tattggcata ttacaacata cgacagtagc aaaaaattat
     1501 catcaaagag gtagtatgac ttacgtgtaa aagcgaaagc ttaaccccaa aaaaatattg
     1561 atctattcaa gtccggttta tttcaggggt ttgattctga aacaagaaaa atgtattaaa
     1621 aaaagcaaaa aacgtaaccc ctgcctgcgt ttcgagcgta tcttctgtta gaaacgagca
     1681 aatggctgta ataaaaaatg taacatataa gaattgactc caacgattta gcttggttgc
     1741 cggccatagc aacatggtta taaaaattag taaacctgtt aagccaaacg atactgcaat
     1801 tgccaaaaac tgattatgcg agcgcaacca aaattgctta aacagttgcg gagtgttaga
     1861 agcataatat ttgtctaact ccagtttatt atcacctgta cctacaccaa aaagcaaatg
     1921 attgctgata atatgcagtg atgctttcca gaatagcagc ctcatggtta atgaatgacc
     1981 acttgggtta ccaccttgcc acgcattgtt tagctcgtat aaggttgcat ggtaacgggc
     2041 tgaaatacta ttgccttgca attgattaac ggatgcaata ccggcctcaa tatttttaat
     2101 atccgtttcg cttaactgca taagcccatt agcatccttt aataagccct ttgatgtcat
     2161 gtatcttagc aaagtatatt ttatccattg cccgcgtgca tccacactat caaaatcaat
     2221 actgctgcgg gtttgccagg cactgtctaa ctcgctataa gccacattaa caaaaatggg
     2281 gtaaccgttt tcgagatcgc cattaaaacg ttcgtgggta tataggttgc ccaaagcagt
     2341 ataaacaggg aggggctgtg tggtttgttt ggtagtattt aagttgtcta aatcattttt
     2401 aatttttatt cctaaaccta tgagtacggt tattatcgct aaaaaaatcg tggctgtgta
     2461 catccacctt ttttgatgta ttaaaaaaca aattacatat ataccaagtg taacacttaa
     2521 aattataagc cctgtaaacg attgaagtat gcacagaaaa aaaacaaacc atattattaa
     2581 aaccgcatac atataattgt aaaacacttt tgtatttgaa taaattataa agtaaataca
     2641 tgcgtaaatg ctcatacaaa ttagcagagc aaagcgtatg
//
LOCUS       DAOMAA010000188         2679 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_32464_length_2679_cov_3.154726, whole genome shotgun sequence.
ACCESSION   DAOMAA010000188 DAOMAA010000000
VERSION     DAOMAA010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32464_length_2679_cov_3.154726"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            360..632
                     /locus_tag="PLO59_03425"
     CDS             360..632
                     /locus_tag="PLO59_03425"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV00182.1"
                     /translation="MAFSQVTDVHSVVYPNPTNGKVMVQFDEAVLETVTVEIINVTGE
                     QVYQTSLKVNTDSNLFELNLFTLSKGVYTIKYRTSHNQWHEKLIVQ"
     gene            1050..>2679
                     /locus_tag="PLO59_03430"
     CDS             1050..>2679
                     /locus_tag="PLO59_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00183.1"
                     /translation="MHNKLQQKNTTYRIGMSWVFAILLSSIQFAWAQVPSTYSFSQTS
                     TTYTPITGTASTAVGDDGTQTGIPIGFNFVYNNVTHTAVGVTTNGAIRPGTGAAPSFS
                     NSLSGNSDVIAGIWDDNNATGGSIIYATNGTAPNRTFTMQWTNIHIGGTGSNTNPTAT
                     FQVVLFETTNAIQIIYGGTSAALVSTTASIGISGNIGNFLSVTPASPNATYSTSTANN
                     SISSATNFPSGTVYNFNPPPACVTAISGSITPSTVTLCAGGSTTLTATGALNDVGSTH
                     QWKVSSTPGGPYTNVSGGTGATTTSHVTGALIAGTYYYVMENTCANCGPCSILSNEVT
                     VVVNSLPSVAVTPNSGSVCNPGSPSIALAASGASTYTWAPAATLSASTGGSVTATPVA
                     STNYTVTGTDGNGCTATGTTSIVYNQGVNLTSVTASPSSVCSGNNSTLTANITAPSLS
                     YCVSTHTSGCSGDNITLVVLDNINNPTSGCGGVSRFTYFNGGGAQTTSLTASHVTSPS
                     STVELLHCTSSPFGPVMATTSTPSSPESGVRSTNW"
BASE COUNT          809 a          534 c          519 g          817 t
ORIGIN      
        1 aaactattat agtctggatg gtttgaacat attattttct tgatttcatg cgtaagaatt
       61 gtttgtttgt gaaaaaaaat tagattggaa gaatctttat atgaagatta attatgtata
      121 caaaaatata atttgtttta agtatttcga aaaccaatgt aaccatattt ggaggtaata
      181 acggtactgc gacagccaat gtcagcggtg gagtaagtcc ttacgcatat ttatggagca
      241 atggacaaac aacacaaata gcctctgcat tagtcgctgg tacctataca gtaactgtta
      301 cagactttaa cggctgtact aaaactaaga gcataaatat tacacaaccg gttgcgcgtt
      361 tggcatttag tcaggtaaca gatgtacact cagtagttta cccgaaccca actaatggta
      421 aggtgatggt acagtttgat gaggctgtat tggaaactgt aaccgttgaa attataaacg
      481 taaccggaga acaagtttat caaacatcat taaaggtgaa tacggattca aatctgtttg
      541 agttgaattt gtttacgctt tcaaaaggtg tttatacaat aaaatatcga acatcacata
      601 atcaatggca tgagaaattg attgttcaat aatttggttt taggttttga aaggctgctt
      661 caaatttttg gagcagcttt ttttatgctt taaataggca tgaaatcaaa aaaatttgtt
      721 tttaaagtgt ttagtggttt aacgtttata ggtgcttaaa gaaaagttta aatttaagat
      781 agctgccata ttactgatta gatacgttta aacgctggtt ttttgaatta aaaagttttt
      841 gaatttgttg ccaataaaat ttggaattaa aaataattac tattttgtag ctctttttca
      901 aaattacccc ctaattctac ttatgagtaa ttacccctac tttggcttaa cagctatagc
      961 tttttattta ttcgctaaac ctttctcgta atcctaaatt acgaacagct tcaatttaga
     1021 ataataaaaa aatccttaaa ccttatatta tgcataacaa actacaacaa aaaaacacta
     1081 cgtatcggat aggtatgtct tgggtgtttg caatactgct aagcagtatt caatttgcct
     1141 gggcgcaagt gccatccact tactcgtttt cacaaaccag tacaacctac acgcccataa
     1201 caggtacagc aagtactgca gttggtgatg atggtacaca aacaggcatc cctataggct
     1261 ttaactttgt gtataacaat gttacccata cggcagttgg tgttaccacc aatggtgcaa
     1321 tacgacccgg aacgggtgca gctccaagtt tttctaatag cttaagtggt aattctgatg
     1381 tgattgccgg tatttgggat gataacaatg ctacaggcgg aagcataatt tatgctacta
     1441 atggcacagc acctaaccgt acttttacca tgcaatggac taacatccat attggtggta
     1501 cagggagtaa taccaaccca acagcaactt ttcaggttgt tttattcgaa acaactaatg
     1561 caatacaaat aatatatggc ggtaccagtg cagcattggt aagcactact gcttcaattg
     1621 gtatttcggg taacattggc aattttttaa gtgtaacacc ggcatcgcca aatgcaacct
     1681 attcgaccag tactgccaat aacagcatta gcagtgcaac taattttcca agtggtacgg
     1741 tatataattt taacccaccg cctgcctgtg ttacagctat aagtggtagt atcacaccgt
     1801 caacagttac actgtgtgcc ggaggttcaa ctaccttaac tgctaccgga gctttaaatg
     1861 atgttgggtc aacacatcaa tggaaggttt cgtcaacacc aggtggacct tacaccaatg
     1921 tttcaggggg tacaggagct accacaactt cgcacgtaac aggtgctttg attgcaggca
     1981 cttattatta tgttatggaa aatacatgtg ccaattgtgg cccctgcagc attttatcca
     2041 atgaagttac agttgtagtg aattcactgc cttccgtagc agttacacca aacagcggtt
     2101 cggtatgtaa tccaggtagc ccttccatag cattagcagc ttcgggagct tctacttata
     2161 catgggcacc ggccgctaca ctttcggcat ctacgggagg ttcggtaacc gccacacctg
     2221 ttgcaagtac caattatacc gttaccggaa ccgatggtaa tggttgtaca gcaacaggta
     2281 caacatccat agtttataac caaggtgtta acctcacatc tgttactgca tcaccgtcta
     2341 gtgtttgttc aggcaacaat agtacgttaa cagctaatat aactgcgcca tcacttagct
     2401 attgtgtctc tacgcataca agcggttgtt caggcgataa cattaccctg gtagttcttg
     2461 acaatatcaa taatccaaca tcgggttgcg gtggtgtttc gcgttttacc tatttcaatg
     2521 gtggtggtgc gcaaacaact tctttaacag catcccacgt tacttcgccc tcttccaccg
     2581 tagaactttt gcattgcaca agttcccctt tcggtccagt gatggcgacg acatcgaccc
     2641 cgtcatcgcc ggagtcaggt gtccgttcaa cgaactggt
//
LOCUS       DAOMAA010000189         2678 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_32502_length_2678_cov_2.643538, whole genome shotgun sequence.
ACCESSION   DAOMAA010000189 DAOMAA010000000
VERSION     DAOMAA010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32502_length_2678_cov_2.643538"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1160)
                     /locus_tag="PLO59_03435"
     CDS             complement(<1..1160)
                     /locus_tag="PLO59_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00184.1"
                     /translation="MKKILLLLLACSTGQTFAQTMYKYGLPKGNDFDRWSVGIIGGMA
                     SMQSDIKAKGDAQNSLNTQSPELIFGGHIGFQLTHSIGFTLRGLSGNLTQELDEKIYV
                     RELDTAAYLNFKSPLTELTFNMQFSFGNISFVKRNTNLHFVVRAGGGLALNKPNLQYS
                     YDKVNYKPVADYGNVSNFVMPLGAGVRYAFGKFNVGIDYDYRMMFTDRLDGLNVTNSD
                     YDGYTSITAQVNYIIGKKKKPMEWINPMELVYNDISEVREKVDALANDKDGDGVADMF
                     DKDSATPQGQKVYGDGSAIDTDQDGIADNLDEDPYSNRGAVVDGSGRELDADNDGVVD
                     SKDKESNTPPGTLVNFQGVAIGNNGSKTANGSNSNTSTTTVAGYFPSIFFDM"
     gene            complement(1265..1939)
                     /locus_tag="PLO59_03440"
     CDS             complement(1265..1939)
                     /locus_tag="PLO59_03440"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HQV00185.1"
                     /translation="MAYYLHLSTMQARYIVIEGNIGSGKTSLATQLQQHYQSELLLEE
                     FAENSFLPKFYENPERWAFPLELSFMADRYNQMQRMLQVIKPSNKLLVADYLFDKSLI
                     FARNNLTAAEMDLYEKFFAIISNQIPKPELIVLIKNSTQQLHTNIAKRGRSYEQQITT
                     SYLENITLGYNRYKQQHADLNWLEINGDNYDFVNNKTHFKNIVKLIETAINTDNTKLF
                     GPLAFG"
     gene            2054..>2678
                     /locus_tag="PLO59_03445"
     CDS             2054..>2678
                     /locus_tag="PLO59_03445"
                     /inference="COORDINATES: protein motif:HMM:NF018378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glucuronyl C5-epimerase family protein"
                     /protein_id="HQV00186.1"
                     /translation="MALSLSMLSFTKPAQVHVFTPTGDFSYLKKGWVLSRCVTVAHWE
                     TDPVFSFDDDGIIMNKKTYHPVVISQYALTVYDAWQRNGSAELKTKFLNQIKYLSNPK
                     FYTQINDTVVAYPYTVNFHDLRAPWYSGLAQAEVLGVLVRYYAITQDKTILPLMVKIK
                     NMMTLPVSKGGLLNTTAEGNVWIEEYPNSAQHLHVLNGFMVTALLLDD"
BASE COUNT          752 a          519 c          532 g          875 t
ORIGIN      
        1 ttcatatcaa aaaagatgga agggaaataa ccggcaacgg ttgttgtact tgtattgctg
       61 ttgctgccat ttgcagtttt gcttccatta ttgccaatag ccacaccctg aaaatttacc
      121 agtgtaccgg gaggcgtatt cgattcttta tccttactgt caaccacacc atcattatcg
      181 gcatctaact cgcggccgct gccgtcaacc acagcgcctc ggttactgta tgggtcttcg
      241 tctaaattat cggcaatacc atcctggtcg gtatcaatgg ccgaaccatc gccatatact
      301 ttttggcctt gcggtgttgc actgtcctta tcaaacatat cggccacacc atcgccatct
      361 ttatcgttgg ccagggcatc cactttttcg cgcacttcgc ttatatcatt atacaccaat
      421 tccattgggt ttatccattc cattggtttt ttctttttgc caataatata gtttacctgt
      481 gcagttatgc tggtatagcc atcataatcg ctgttggtta cgtttaagcc atctaaccta
      541 tcggtaaaca tcatgcgata atcgtaatca atacccacat taaacttacc aaaagcatag
      601 cgcacaccgg cacctaatgg cattacaaag ttgcttacat taccatagtc ggctacaggt
      661 ttgtagttta ctttatcgta actgtattgc aaattgggct tgtttaaagc caagccgcca
      721 cctgctcgaa ccacaaaatg caagttggta ttacgtttta caaaagatat gttaccaaac
      781 gaaaattgca tattaaaagt aagttcggtt aaaggcgatt taaagtttaa gtatgctgcg
      841 gtatctaatt cacgcacata aattttttcg tctaattcct gcgttaagtt gccgcttaag
      901 ccccttaaag taaagccaat actatgtgtt aactgaaaac caatatgacc gccaaaaatt
      961 agttcgggcg attgggtgtt taacgagttt tgggcatctc ctttggcttt aatatcgctt
     1021 tgcatgcttg ccataccacc aataatgcct accgaccaac ggtcgaagtc attacctttt
     1081 ggtaagccgt atttatacat ggtttgtgca aatgtttgtc cggtactgca agcaaggagc
     1141 agtagtaaaa tttttttcat ttattaatat tttgtatgat atctgatatg cttttgaagg
     1201 catattaatt gatgggagcg gctgcaatat taaaaactta atagttaaag ctaaaggcaa
     1261 attatcaacc aaaagctaaa ggaccaaaca gctttgtatt atcagtgttt atagctgttt
     1321 caattaattt aacaatgttt ttaaagtgtg ttttattgtt aacaaaatcg taattgtcgc
     1381 catttatttc gagccagttt aaatcggcat gctgttgctt gtaccggtta taaccgaggg
     1441 ttatgttttc taaatagctt gtagtaattt gttgctcgta actgcggccg cgtttggcaa
     1501 tattggtatg caattgttgg gtgctgtttt taatgagtac aattaattca ggctttggaa
     1561 tttgattact gataatggca aaaaactttt catacaaatc catttcggct gctgttagat
     1621 tattgcgtgc aaaaatgagc gacttgtcaa ataaataatc ggctactaat aatttgttgg
     1681 atggtttaat tacttgcaac atgcgctgca tctgattata ccgatcggcc ataaagctaa
     1741 gttcgagcgg aaaagcccag cgttcggggt tttcgtaaaa cttaggcaaa aaggagtttt
     1801 cggcaaattc ttctaataat aattcgcttt gataatgttg ttgtagttgc gtggctaagc
     1861 tggttttgcc actgccaata tttccttcga taactatata ccgcgcttgc atggtgctaa
     1921 ggtgcaggta atatgccaat tgtaaaagcc cttaatactt aatttattcg tttttaaaga
     1981 tacttttggc ggccattatt ggatgttaat gaagtatcga aaaatattgt accgtatttt
     2041 atgcttatgc ggtatggctt tatcgctaag catgctaagc tttaccaaac ctgctcaggt
     2101 gcatgtattt acacctacag gcgattttag ctatctgaaa aaaggttggg tgttatcgcg
     2161 ctgtgttacc gttgcccatt gggaaaccga ccccgtattt agttttgatg atgatggcat
     2221 catcatgaat aaaaaaacgt atcacccggt agttatcagc caatatgcgt taacggttta
     2281 tgatgcctgg cagcgtaacg gcagtgccga gctgaagaca aaatttttaa atcagattaa
     2341 atatctatcc aacccaaagt tttatacgca aataaacgat acggtggtag cttatcctta
     2401 taccgttaat tttcacgact tgcgtgcgcc ctggtacagt ggcttggcac aggccgaagt
     2461 actgggcgtt ttggtacgtt attatgcaat aacacaagac aaaactattt tgccattgat
     2521 ggtaaaaatt aaaaatatga tgacgctgcc tgtttcgaaa gggggcttgt taaatactac
     2581 tgccgaagga aatgtttgga ttgaagaata tcccaactcg gcacaacatt tacatgtgct
     2641 taatggtttt atggttacgg cattgttgtt agatgatt
//
LOCUS       DAOMAA010000190         2672 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_32642_length_2672_cov_2.618647, whole genome shotgun sequence.
ACCESSION   DAOMAA010000190 DAOMAA010000000
VERSION     DAOMAA010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32642_length_2672_cov_2.618647"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="PLO59_03450"
     CDS             complement(<1..245)
                     /locus_tag="PLO59_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008240087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQV00187.1"
                     /translation="MFVSGFTFIRNAITYDYPIIEAITSILPICDELIIAVGNSDDNT
                     LALIESINSPKIKIIETVWDDNLRQGGHVLAVETNKA"
     gene            complement(268..1620)
                     /gene="purB"
                     /locus_tag="PLO59_03455"
     CDS             complement(268..1620)
                     /gene="purB"
                     /locus_tag="PLO59_03455"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019387141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HQV00188.1"
                     /translation="MNTTVLHAVSPIDGRYQTKCDSLTNYFSEFALMKYRLRIEVEYF
                     IALCKLPLPQLEKVPVISFDSLRNMYLLFNDTDALAIKQIELVTNHDVKAIEYFMKQR
                     FEALGLDAFKEFIHFGLTSQDINNTAIPLSIKEFIADGYLPQLNTLIEKLHGLAKQWQ
                     PIAMLARTHGQPASPTTLGKELYVFVERLTVQRNQLLQIPNSAKFGGATGNFNAHVIA
                     YPKIDWIDFGNNFVATLGLQRSQTTTQIEHYDNLAALMDVLKRINTILMDACRDFWTY
                     ISMNYFGQQIKAGEVGSSAMPHKVNPIDFENAEGNLGIANALFEFFASKLPISRLQRD
                     LTDSTVLRNIGVPLAHTTIALSSLMRGLQKLKLNDAVLKADLDENWAVVAEAIQTILR
                     RENYPNPYEALKELTRTGQAITSKTIASFIDSLDINADIKVELKAINPHNYTGINKNF
                     "
     gene            1796..2548
                     /locus_tag="PLO59_03460"
     CDS             1796..2548
                     /locus_tag="PLO59_03460"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HQV00189.1"
                     /translation="MKYILSVLTLIGLTHASLAQKPSTVGPTTKAKSQVYPLDTIRTQ
                     VKEIPGGEMWTMRGFRGEISGKGLMVNGKKEGVWRRYHSNKCLAKLENYKNGELNGTC
                     ISIGQNGNIETEENYNNGKYDGLVTFYSFSGNIKKEENYSDGKLHGKRCLYYDNEKLQ
                     EETEYILGIKSGLARWYNQEGVCVIEYTYKNGSLQGPAKEFFAGGQLFKSGNYNNDVE
                     IGDWLEYKENGELVKTITFKNGEVLKETPAKK"
BASE COUNT          870 a          458 c          530 g          814 t
ORIGIN      
        1 aaagctttat tggtttcaac ggctaaaaca tggccgcctt gccgtaagtt gtcgtcccaa
       61 acggtttcta ttattttaat ttttggcgag tttatagact caattaaagc aagcgtatta
      121 tcatcactat taccaacagc tataattaac tcatcacaaa ttggtaggat agatgttatc
      181 gcttctatta ttgggtaatc atacgttatg gcattgcgta taaaagtgaa accggataca
      241 aacatgattt attagataat actatactta aaaattttta ttaatgcccg tatagttatg
      301 tgggtttata gcttttagct caacttttat atctgcgtta atatctaaac tatcaataaa
      361 tgaggcaatt gtttttgaag taattgcttg cccggtacgc gtaagttctt ttaaagcttc
      421 gtatgggtta gggtagtttt ctctgcgcaa aatagtttga atagcctcgg caacaacggc
      481 ccagttttca tctaaatcag ctttcaaaac agcatcattc agttttaatt tttgcaggcc
      541 ccgcattaat gatgatagtg caatggtggt atgcgccaaa ggcacgccta tgttgcgtag
      601 cacggtcgag tcggttaagt cgcgttgtaa acgtgatata ggaagcttgg aagcaaaaaa
      661 ctcaaacaag gcatttgcta ttcctaaatt accctctgca ttttcaaaat caatggggtt
      721 aactttatgc ggcattgccg atgagcctac ttcgcctgct ttaatttgtt gtccaaaata
      781 attcatcgaa atataagtcc aaaaatcgcg acaggcatcc attaaaattg tgttgatgcg
      841 tttcaataca tccattaatg ccgctaaatt atcatagtgc tcaatttgag tagtagtttg
      901 cgatcgttgc aaccccaacg ttgcaacaaa gttgtttcca aaatcaatcc aatcaatttt
      961 cggataagca ataacatgag cattaaaatt accggtagca ccaccaaact tagccgagtt
     1021 tggtatttgt aacagttgat ttctttgcac cgttaaccgt tcaacaaaaa cataaagctc
     1081 cttgcccaat gtagttggcg atgccggctg gccatgggta cgtgcaagca tagcaattgg
     1141 ttgccattgc tttgctaagc catgcagttt ttctatcaat gtgtttagct gtggtaaata
     1201 accatcggca ataaattctt ttattgataa gggtatggct gtattgttta tatcctgcga
     1261 ggtaagtccg aaatgaataa actccttaaa tgcatctaaa cccaaagctt cgaacctttg
     1321 tttcataaag tattcaatag ccttaacgtc atgattggta accaactcta tttgtttaat
     1381 agccaatgca tccgtatcat taaaaagcaa atacatgttg cgcaaactat cgaaactgat
     1441 taccggtact ttttctaatt gtggtaaagg cagtttacac aatgcaataa aatactcaac
     1501 ttcaatgcgc aagcggtatt tcattaatgc aaactcgcta aagtagttgg ttaatgagtc
     1561 gcatttggtt tggtaacgtc catcaatcgg tgaaaccgca tgcaatactg tggtgttcat
     1621 tgttttttga ttttaaaaaa gagcgcaaat gtagccaaat gaccttagca cactaatgca
     1681 tgtttttaac tgaccggcat cggtttttaa attaaaaaca gctatttcat acatacttaa
     1741 ttttgattgc aggctaagct ttattattgg cctattaatt ttattaagta acaaaatgaa
     1801 atatatttta agtgtgttaa cgcttatagg attaacgcat gcaagcctgg cacaaaagcc
     1861 atcaacggta ggccccacta caaaagctaa gtcgcaggta tatccattag ataccatacg
     1921 cactcaggta aaagaaatac ccggaggcga aatgtggact atgcgcggct tcagaggcga
     1981 aatttcgggc aagggtttaa tggttaatgg taagaaggaa ggtgtttggc gcaggtatca
     2041 cagcaataag tgcttagcca aattggaaaa ctataaaaac ggtgaactaa acggaacgtg
     2101 tattagtata ggccaaaacg gcaatatcga aaccgaggaa aattataata atggtaaata
     2161 cgatggctta gtaacttttt attcgtttag tggcaacatt aaaaaagaag aaaattacag
     2221 cgatggaaaa ttgcatggta aacgttgtct atattatgat aatgaaaaac tacaggaaga
     2281 aacggagtac attttgggca taaagtcggg gttagcaaga tggtataatc aggagggggt
     2341 ttgtgtaatt gaatacactt acaaaaatgg atcgctgcaa ggcccggcaa aagagttttt
     2401 tgccggagga caattattta aaagtggcaa ttataacaac gatgttgaaa ttggcgattg
     2461 gctcgaatac aaggaaaatg gggagctggt aaaaacgatt acttttaaaa atggagaagt
     2521 tcttaaagaa acacccgcta aaaaataaac gtgcaattcg gtatgtaaat tgggatcaac
     2581 caacatattt taaaacaaaa gtgttttaga tgttgtataa accgaataaa ttttactttt
     2641 gcttaattga aatgtttgct cggagttatt gg
//
LOCUS       DAOMAA010000191         2666 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_32783_length_2666_cov_2.851781, whole genome shotgun sequence.
ACCESSION   DAOMAA010000191 DAOMAA010000000
VERSION     DAOMAA010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32783_length_2666_cov_2.851781"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2666)
                     /locus_tag="PLO59_03465"
     CDS             complement(<1..>2666)
                     /locus_tag="PLO59_03465"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase PknK"
                     /protein_id="HQV00190.1"
                     /translation="TDSKGNRHVLKVLRTLTPNPDQILSFNNEYDFTKDLKISGVRKA
                     LFTTKFENKLAIGFEFIEGDTLENKILSQQISVLEQLKIYVTIADILTQIHNQGIIHK
                     DINGRNILWDSQTQQPVLIDFGISTHIDLINTNLGNPDRVKGTLSHMSPEQTGRVNRK
                     IDSRSDLYSLGVTMYKSLTGQLPFIASDSLELVHYQLAKSIPQAHLVNAKIPVAVSNI
                     IIKLMAKNAEDRYQSAVGLKHDLAYCIRQIENGETHIDFKIGQHDVSGKFQIPQKLYG
                     RDKEIDVLLKSFDAVSQGSTEWFLVSGYSGIGKSVLIGELYKPITERRGHFAEGKFDQ
                     YQRNIPYYAFTLAINEFCRLLLTESKAKLQYWQNLIQQGVGENGSILIELAPALADII
                     GEQEEAPKLDAMEALNRFNITFVNFIRAIAQPEHPFVFFIDDLQWADVGSLNLIKTMV
                     CDLSLPHLMVIGAYRDNEVTPGHPLLHTIDAAIKDKAIVKSIQLKPLLAEHIFQLVKD
                     VLHTEDKITLSLTELIFSKTQGNAFFTIEFLKSLYHFDLIVFNQEKNLWDVNIDEIKK
                     RKITDNVIDFLIAKISELPLPTQHVLQLASCIGGLFDINLLSTLYDKPAADTLNDLWP
                     AVLEGLVLPLNENYKLIEHIEVSQQAKFEFAHDRIQQASFTLMPATRQAAVHLQIGRL
                     LLSNHPDTDDVIFDIVNHCNEGITLINIQTEKDKLAQLNLQAAIQAKNSSAYKSASVY
                     IDAAQKLAGDAIWQSDYDFALKLFTNHAEIDYLNGNLEQSQNSLLNCIERAQTAAEKS
                     SIYFMLMQNQNNSTQYYEAIESARQGLQLLNFTFPQKDDCPALIPDEMGKIISYFEAH
                     GVESVFDKQTIQDQGILSTINILDNLSPPTYV"
BASE COUNT          743 a          497 c          497 g          929 t
ORIGIN      
        1 tacataggtt ggtggcgata gattatccaa aatgtttatg gtacttaaaa taccttgatc
       61 ttgtatggtt tgcttatcga aaaccgactc aacaccatgg gcttcgaaat aggaaattat
      121 tttgcccatt tcgtcaggta ttaatgcagg gcaatcgtct ttttgcggaa atgtaaagtt
      181 aagcaactgc aacccttgcc gtgccgattc gatagcttca tagtattgcg tgctgttgtt
      241 ttgattttgc atcagcataa aatatatgct gcttttttcg gctgctgttt gtgcacgctc
      301 tatgcaattt aataaactgt tttgcgattg ctctaaatta ccattcaggt aatcaatttc
      361 ggcatggttg gtaaacagtt ttaatgcaaa gtcataatcg ctttgccaaa tagcatcgcc
      421 tgctaatttt tgtgctgcat caatatatac cgatgctgat ttgtaggcac tgctgttttt
      481 tgcttgtatg gctgcttgca aattaagctg cgccaacttg tctttttcgg tttggatatt
      541 aattaatgtt atgccttcgt tacaatggtt tacgatatcg aatataacat cgtccgtatc
      601 gggatgattg cttaaaagca accgtcctat ttgcagatgt acggccgctt gcctggtagc
      661 tggcattaat gtaaaacttg cttgttgtat tctgtcatgt gcaaattcga atttggcttg
      721 ttggcttacc tcaatatgtt caatcagttt ataattttcg tttaacggca ataccaaacc
      781 ctctaacact gccggccata agtcattaag tgtatcggca gcaggcttat catataatgt
      841 tgacaacaga tttatatcaa acaacccacc aatgcagctg gctaattgta atacatgttg
      901 ggttggcaag ggtagttcgc taatttttgc aattaaaaaa tcaattacgt tatcggtaat
      961 ttttcgcttt ttaatttcat caatgtttac atcccataaa tttttttctt ggttaaacac
     1021 gattaaatcg aaatgataga gcgattttaa aaactctatt gtaaaaaatg cattaccctg
     1081 tgttttcgaa aatattaatt ctgttaagct taatgtaatt ttatcttcag tgtgcagtac
     1141 atcttttaca agttgaaaaa tgtgttcggc taataatggt tttaattgaa ttgactttac
     1201 aattgcttta tccttaatag cagcatcaat tgtatgtaac aacgggtggc ccggtgtaac
     1261 ttcgttatcg cggtatgcgc caattaccat caggtgcggc aaacttaaat cacataccat
     1321 ggttttaata agatttagcg aacccacatc ggcccattgc aaatcatcaa taaaaaaaac
     1381 aaatggatgc tcgggttgcg ctattgcgcg aataaaattt acaaatgtta tattgaaacg
     1441 attgagtgcc tccatagcat ctaacttggg tgcttcctct tgctcgccaa taatatcagc
     1501 caaggcaggt gctaattcaa ttaaaatgct gccgttttcg cctacacctt gttgaatcag
     1561 gttttgccag tactgtagtt tagccttgct ttctgttagc aacaatctgc aaaattcatt
     1621 tatagcaagt gtaaatgcat agtatgggat attgcgttga tactggtcaa atttgccttc
     1681 ggcaaaatgg ccccttcgct cagttatggg cttatacaac tcgccaatga gaactgattt
     1741 acctatgcct gagtaacctg aaactaaaaa ccactctgta ctaccttgac taactgcatc
     1801 aaacgatttt agtaagacat ctatttcttt atcgcggcca taaagttttt gtggaatttg
     1861 aaattttccg gaaacatcgt gctggccaat tttaaaatct atatgcgttt caccgttttc
     1921 tatttgacgg atgcaatagg ctaaatcgtg cttcagacct actgcacttt gatagcggtc
     1981 ttcggcattt tttgccatca acttgataat aatatttgaa acagcaacgg gtatttttgc
     2041 attgaccagg tgtgcctgcg gtattgactt ggcaagttga taatgtacca gttctaacga
     2101 gtcgctggct ataaatggca attgaccggt tagcgatttg tacatggtaa cacccaatga
     2161 atataaatcg ctgcggctgt ctattttacg gtttaccctg cctgtttgtt cgggcgacat
     2221 gtgcgatagt gtgcccttaa ccctatcggg atttcctaaa tttgtattta ttaaatcaat
     2281 atgtgtgcta ataccaaaat caattagtac gggttgttgt gtttgcgaat cccataatat
     2341 atttcgtcca ttaatatctt tatgtataat gccctgattg tgaatttgcg ttaatatatc
     2401 ggctattgtt acataaattt ttaattgctc aagcactgaa atttgctgac ttaaaatttt
     2461 attttcaagt gtatcgcctt caataaattc gaacccaatg gccagtttgt tttcaaactt
     2521 agttgtaaac aaggcttttc gtacgcctga tatttttaag tctttagtaa aatcgtattc
     2581 attattaaaa cttaaaatct gatcggggtt tggtgtaagc gtacgtaaaa cctttaatac
     2641 gtgacggtta cctttgctat cagtaa
//
LOCUS       DAOMAA010000192         2650 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_33149_length_2650_cov_2.540655, whole genome shotgun sequence.
ACCESSION   DAOMAA010000192 DAOMAA010000000
VERSION     DAOMAA010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33149_length_2650_cov_2.540655"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2650)
                     /locus_tag="PLO59_03470"
     CDS             complement(<1..>2650)
                     /locus_tag="PLO59_03470"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HQV00191.1"
                     /translation="TRKVSYTPGFSYLRGFQDFYGLSLSAYTTGEGFAGPVFTNTSNP
                     TFATLLHTPNAVAGNTPMRLKMRVQGANLRDHFLNVTLNNVPVFNTAFVQYNVVDTNL
                     LLPNIVLSNTINAEFTALPNPDNSVGSMAVNYMELYYNHSFNFATETAAQYHIAARGN
                     FATPFTRWDISNLNFQQPILLLWANDTIKRIALYGSGNNFQTLLPTYGFDKNCVLLDS
                     ASQTYTNDAAINFDMKPVRAGNPLAPVAHFTNFAFNPNAQLILITNQKLWAAAETYKT
                     YRQSNPYNTYTTLLADANELYDQFANGISKHPLAIKNFIRYTRDNFTIKPEQVFLIGK
                     SIKNFLHITNQGIPGFDDDNLVPTFGEPPSDLMFTTGLLTNDSVTRPALPVGRLSAAT
                     PQDVTDYLEKVMAYEAQPNNVWMKNVLHFAGGVSIQQRQDIESKLAEYATIISDSAFG
                     GYVRTISKTVTDPIQIALSNQLQQIIDSGVSLMTFYAHAGASTFDISTDEPASWKNKD
                     RYPLVLGNSCYVGDAHQKGRAAAENFVLQKNKGAIGFIGSASVNYIENLYPYAKSLYQ
                     HISLYNYGNSIGRCLIKGIDTTWANYNAGFYTETVTIGMNLQGDPTLIINAAEKPDFE
                     ITASDISFNPTSVTAIADSFDVNIVIKNIGMATNSAYIVEVSRTFGNGTTTDSVYVRP
                     ALTYTDTLKLRFAVDAAQGPGLNLFTVKVDPFGAIAELNDSTNNEAQQQLYILSNDIT
                     PIYPPPFAIVGNNSFQLQASTVNQFAPVTRYVFELDTTDAFNSAILWHDTVTAAGGYI
                     TKNLPYTPVANTVYYWRVSRDSVAGDPIHPAWNEASFIYIANQNGWSQAHFYQFKNNT
                     YTNINYNRPQQRWDYVSTKSVLKAET"
BASE COUNT          770 a          481 c          579 g          820 t
ORIGIN      
        1 ttggtttcgg cttttaatac acttttggta cttacgtaat cccaccgttg ctgtggcctg
       61 ttataattaa tgttggtata agtgttgttt ttaaattgat aaaaatgtgc ctgactccat
      121 ccgttttgat tggcgatgta aataaacgag gcttcattcc aggcagggtg tatcgggtcg
      181 cctgcaaccg aatcgcgact tacgcgccaa taataaaccg tgtttgcaac cggagtataa
      241 ggtaagtttt tagtaatgta tccgcctgct gcagttacgg tatcatgcca aagtatagca
      301 ctgttaaatg catcggttgt gtctaattca aaaacataac gtgttacagg tgcaaactga
      361 ttaacggtac ttgcctgtaa ctgaaaactg ttgttgccaa caatggcaaa tggaggtgga
      421 tatatgggtg taatatcatt acttaaaata tatagttgtt gttgcgcttc attattggtc
      481 gagtcgttaa gctcggctat agcgccaaag ggatcaacct ttacagtaaa caaattaagt
      541 ccgggtcctt gtgcggcatc tactgcaaaa cgtaatttta atgtatcggt ataagttaat
      601 gcaggcctta cgtaaacact gtcagttgtt gtgccgttgc caaaagtacg gctaacttcc
      661 actatatacg cactgttggt tgccatacca atatttttaa taacaatgtt tacatcaaag
      721 gagtcggcaa tggctgtaac cgatgttggg ttaaaactta tgtccgaagc cgttatttca
      781 aaatcgggtt tttcggctgc attaataatt aaagttggat cgccttgcag attcatacct
      841 atggtaacgg tttcggtata aaaaccggca ttgtaatttg cccatgtggt atcaatacct
      901 ttaattaaac aacgtccaat ggagttgcca taattatata agcttatatg ctgatataat
      961 gatttggcat aggggtataa gttttcgatg tagttaaccg atgcactgcc aataaaacca
     1021 atggctcctt tgtttttttg aagcacaaag ttttcggcag cggcacggcc tttttgatgt
     1081 gcatcgccaa cgtaacatga gttgccaagc actaaaggat agcggtcttt atttttccag
     1141 gatgcgggtt catcggtact gatatcaaat gtggatgcac ctgcatgtgc ataaaaagtc
     1201 attaaactaa caccactatc aataatttgt tgcagttgat tgcttaaagc aatttgaatt
     1261 gggtcggtta cggttttact gatggtgcgt acatagccac caaaggccga gtcggaaata
     1321 atggttgcat actcggctaa tttcgattcg atatcctgac gctgttgtat gcttacgcca
     1381 cctgcaaaat ggagtacatt tttcatccaa acgttattag gctgcgcttc ataagccatt
     1441 actttttcta agtaatcggt aacatcttgt ggtgtggcag cacttaaacg gccaacgggt
     1501 aatgccggcc gggtaacgct gtcgttggtt aacaagcctg tggtaaacat taaatcgctg
     1561 ggaggctcac caaaagtggg cactaaatta tcgtcatcaa aacccggtat gccctgattg
     1621 gtgatatgca aaaagttttt aatactcttt cctattaaaa atacctgttc cggtttaatg
     1681 gtaaagttat cgcgcgtgta gcggataaaa tttttaatgg ctaaaggatg tttacttata
     1741 ccgttagcaa actggtcgta cagttcattg gcatcagcca ataatgtagt gtatgtattg
     1801 taagggttac tttggcgata ggttttatac gtttcggcag cagcccaaag cttttgattg
     1861 gttattaaaa taagttgtgc attggggtta aaggcaaaat tggtaaagtg tgctacaggt
     1921 gctaacggat taccggcacg taccggtttc atatcaaagt ttatggctgc atcgtttgta
     1981 tatgtttgtg atgccgagtc gagtaaaaca caatttttat caaagccata agtgggtagc
     2041 agggtttgaa aattattgcc gctgccatac aatgcaatac gctttatagt atcgttagcc
     2101 cacagtaata atatgggttg ttgaaaattt aaattactta tatcccaacg cgtaaaaggt
     2161 gttgcgaaat ttccacgtgc agcaatgtga tattgtgcag ctgtttctgt tgcaaaatta
     2221 aagctatggt tataatataa ctccatgtaa tttacagcca tgctccccac tgagttatcg
     2281 ggatttggta atgcggtaaa ctcggcattt atagtattgc ttaatacaat gttaggtaac
     2341 aataaattag tgtcaacaac attatactgc acaaaagctg tattaaaaac aggcacatta
     2401 tttaaggtaa catttaaaaa atgatcgcgc agattggcgc cttgcacacg catttttagg
     2461 cgcatgggtg tatttcctgc aacggcatta ggggtatgta aaagcgtagc aaaagttggg
     2521 ttagatgtat tggtaaaaac aggccctgca aaaccttcgc ctgtggtgta agcacttaaa
     2581 ctgaggccat aaaaatcctg aaaaccacgc aagtaactaa aacccggagt gtaggaaact
     2641 ttacgtgtaa
//
LOCUS       DAOMAA010000193         2647 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_33212_length_2647_cov_3.230710, whole genome shotgun sequence.
ACCESSION   DAOMAA010000193 DAOMAA010000000
VERSION     DAOMAA010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33212_length_2647_cov_3.230710"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..1591)
                     /gene="rny"
                     /locus_tag="PLO59_03475"
     CDS             complement(23..1591)
                     /gene="rny"
                     /locus_tag="PLO59_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010602424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HQV00192.1"
                     /translation="MDENLIITIVATVAGLLVGVAITYFMVNKTNKGKEEAAQKQAQA
                     ILTEAEAKAEVVKNQKMMEAKEKFYQLKSEHEKSITEKEKQIAVAENRIKQKEKEASQ
                     KSEQLSRKQTEADAIQQSLKTQLEQVEKRKEELNKAHEKQVQQLEKIAGLSQTDAKAQ
                     LVEALKAEAKTAAMSHIKDTMDEAKLTANKEAKRIVIQTIQRVATEHAIENAVTVFNI
                     DSDEVKGRIIGREGRNIRALEAATGIEFIVDDTPEAIILSGFDPVRREIARLALHQLV
                     SDGRIHPARIEEVVAKVKRQLDEEIIEVGKRTTIDLGIHGLAPELIRMVGRMKYRSSY
                     GQNLLQHSREVANLCAIMATELGMNVKLAKRAGLLHDIGKVPDDEPELPHAILGMKLA
                     EKFKEHPEVVNAIGAHHDEIEMTSLISPIVQVCDAISGARPGARREMAEQYMKRLKDL
                     ETLATSYHGVEKSFAIQAGRELRVIVQSEKVSDKQAETLSFDIAQKIQTEMTYPGQVR
                     VTVIRETRSVNIAK"
     gene            complement(1891..2190)
                     /locus_tag="PLO59_03480"
     CDS             complement(1891..2190)
                     /locus_tag="PLO59_03480"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HQV00193.1"
                     /translation="MDTTSITVSIGNRTYPLKTSITEVETVRAAAQSINQLIERFEVD
                     YKITDKQDLYSMCLIQMATELEKQKEAYAQNNSQMHNSLDSMLAMMNNHLTTNVL"
     gene            complement(2193..2501)
                     /locus_tag="PLO59_03485"
     CDS             complement(2193..2501)
                     /locus_tag="PLO59_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00194.1"
                     /translation="MDELNQKINALYGSIEKMIHLQQQLLIENHKMLELNQELIAKIE
                     DQNLTIHQLDEKLKAAKLVQAITSNRTDIDKTDLLIQIDNFINQIDTCMKQLSNEKHN
                     "
BASE COUNT          672 a          518 c          456 g         1001 t
ORIGIN      
        1 taaatttaaa ttttacaata aattattttg caatgtttac cgaacgggtt tcgcggatta
       61 cggtaacgcg tacttgtccg ggataagtca tttcagtttg aattttttga gcaatatcaa
      121 acgataatgt ttcggcttgt ttatcgctca ctttttcgct ttgtacaata acgcgtaatt
      181 cgcgaccggc ttgtattgca aacgactttt caacaccatg ataagaggta gctaaagttt
      241 ctaaatcttt taagcgtttc atatactgtt cggccatttc gcgtctggca ccgggtctgg
      301 caccactaat ggcatcgcat acctgaacaa ttggcgaaat taaactggtc atttcaattt
      361 catcatggtg ggcaccaatt gcattaacca cttcgggatg ctctttaaac ttttcggcca
      421 gtttcatacc taaaattgca tgtggcaact ccggttcgtc atcaggtact ttgcctatat
      481 cgtgaagcaa tccggcacgt tttgccaact taacattcat ccctaactca gttgccataa
      541 tagcacaaag gttagcaact tcacgtgagt gttgcaataa attctgtcca taggatgagc
      601 ggtatttcat gcgcccaacc atacgaatta actcaggtgc taaaccatgt atgcctaaat
      661 caatggtggt acgtttaccc acttcaatta tttcttcgtc taattgacgt ttcactttgg
      721 ccacaacctc ttcaattcgc gcggggtgta tgcgcccatc gctaacaagt tggtgcagtg
      781 ccaagcgtgc tatttctctt cgcaccggat cgaaacctga aagtatgatg gcttccggtg
      841 tatcgtcaac tataaattca attcctgttg cagcttctaa tgcacgtatg tttctacctt
      901 cacggccaat tatacggcct ttaacttcat cgctatctat attaaaaacc gtaacagcgt
      961 tttcaatagc atgctcagtt gctacacgtt gtatggtttg aataacaatg cgcttcgcct
     1021 ctttgtttgc tgtaagtttt gcctcgtcca ttgtgtcttt tatatgactc atggcagccg
     1081 tttttgcttc ggcttttaaa gcttcaacca attgcgcttt tgcatcggtt tgacttaaac
     1141 ctgcaatctt ttctaattgt tgtacttgtt tttcgtgtgc tttattgagt tcttctttgc
     1201 gtttttcaac ttgttcgagt tgtgttttaa gactttgctg aatggcatcg gcctcagttt
     1261 gtttacgtga aagttgttcc gacttttggc ttgcctcttt ctccttttgt ttaattcgat
     1321 tttcggctac ggcaatttgt ttttcctttt ctgtaattga tttttcatgc tccgatttaa
     1381 gttgataaaa cttctccttt gcttccatca ttttttggtt tttaaccact tcggcttttg
     1441 cttcggcttc ggtaagtatg gcttgagctt gtttttgggc tgcttcttcc tttcccttat
     1501 tggttttatt taccataaaa taggtaatag ccacaccaac taaaaggccg gctactgttg
     1561 ctactatagt tattattagg ttttcatcca tatcgcaagt ttatttacta attgatttta
     1621 ttattgttat tttaaagtat atcatatatg ggcataaaaa agcccgacaa aattctgcac
     1681 cattattaaa ccccgaacta ttagggctga agctgcccaa acgtttacgc tacttatgtt
     1741 actttttaag cgaacttaca agtgattaaa gtctgcatta gtgtttgaaa gcttattgct
     1801 tcaatggtat tttaatgttg agtttaaaat agaaatcaga atttcatcag gcctatattt
     1861 gcaaaacgtt tgcaataaac cttttgttta tcaaagaacg ttggtagtta aatgattatt
     1921 catcatggct aacatactat ctaaactatt gtgcatctgc gaattatttt gcgcgtatgc
     1981 ttctttttgt ttttcaagtt ctgtagccat ttgaatcaga cacatactat ataaatcctg
     2041 cttatcggta attttataat caacctcaaa gcgttctatt aactgattta ttgattgtgc
     2101 tgcagctcta acagtttcaa cttcggtaat cgaagttttt aaaggatagg tcctatttcc
     2161 aatcgaaact gtaattgagg tggtatccat tgttagttgt gtttttcgtt gctcagttgt
     2221 ttcatacaag tatcaatttg attaataaaa ttgtcgattt gaattagtaa atcggtttta
     2281 tcaatatccg ttctgttgct tgttattgcc tgaactaatt ttgctgcttt taacttttca
     2341 tctaattgat gaatggtaag gttctgatct tctattttag ctatcaactc ttgatttaat
     2401 tccaacattt tgtggttttc aatcaatagc tgttgctgca aatgtatcat tttttcaatg
     2461 ctgccgtata acgcatttat tttttgattc aattcgtcca tagcttttgc tatccattaa
     2521 attaaacgtt ctaaaaataa gaaaccccgg ttagtccggg gtttctttat aaataagttt
     2581 aaaaagttat tactcagtaa caaccatttt acgaactaat ttctgaccat cattttcaag
     2641 tgtgtag
//
LOCUS       DAOMAA010000194         2647 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_33219_length_2647_cov_2.477238, whole genome shotgun sequence.
ACCESSION   DAOMAA010000194 DAOMAA010000000
VERSION     DAOMAA010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33219_length_2647_cov_2.477238"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="PLO59_03490"
     CDS             <1..655
                     /locus_tag="PLO59_03490"
                     /inference="COORDINATES: protein motif:HMM:NF024045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HQV00195.1"
                     /translation="GEFTWRAFMAGKLDLAQAEAVADVIAADSKSNHQIALQQLRGGF
                     SSDIKKLRESLIHFASLIELELDFAEEDVTFASRQQLTQLVSNMHQTIDALAQSFEIG
                     NVMKHGIPVVIAGMPNVGKSTLLNALLNEERAIVSEIAGTTRDTIEEQINIEGLNFRF
                     TDTAGIRQTVDAIEALGVERTYQKLKLSAVVIYMFDPKHVTPVEVLKTLDELKSNLC"
     gene            649..1020
                     /locus_tag="PLO59_03495"
     CDS             649..1020
                     /locus_tag="PLO59_03495"
                     /inference="COORDINATES: protein motif:HMM:NF024045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00196.1"
                     /translation="MLNKTDTIPESELVPFNQIAEISFISAKQKWQIEKLIQLLLNKV
                     QHVKVSLDSTIVVNVRHKEALDNTSQALQKVLHGIETQISGDFLASDIREALYHLGLI
                     TGEIATNDLLENIFSRFCIGK"
     gene            1230..2138
                     /locus_tag="PLO59_03500"
     CDS             1230..2138
                     /locus_tag="PLO59_03500"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HQV00197.1"
                     /translation="MKSFIWAFAFSIFPTILYAQVGSIINLGVVKSKYASGYNVDVWL
                     PANYSASKQYAVIYMHDGQMLFDSTTTWNKQAWMVQNVVANLMAENKIVPAIIIAIHN
                     NGQSRHADYTPQIPFKKLKRQQQTAMYHDTTAYGNNVFANFKVNSDNYLKFLVNELKP
                     LIDKRFSTYTDKSQTIIMGSSMGGLISLYAICQYPEIFGKAACMSTHWPLVFKTDNNP
                     FPDAMLQYLIGKLPQPESHMLYFDMGTMGIDSLYESTQIQVDALCIDIGYNSNNFMSV
                     VFEGADHNEKAWHKRLHIPLQFLLKP"
     gene            2181..>2647
                     /locus_tag="PLO59_03505"
     CDS             2181..>2647
                     /locus_tag="PLO59_03505"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HQV00198.1"
                     /translation="MHYPYQIKSQQQYENDYALSIKNPKEFWANVAQHFTWFKPYDTV
                     LNWEFNTPDVKWFEGGKLNITYNCLDRHAATHPDKVAFIFEPNDINQRTQKITYAQLL
                     QQVSQTCYALQSIGVQKGSRVIIYMGMVPQLAITLLACARMGAVHSVVFGGFSA"
BASE COUNT          874 a          481 c          511 g          781 t
ORIGIN      
        1 aggcgagttt acctggcggg cttttatggc cggtaaatta gatttagcac aagccgaagc
       61 agttgccgat gtaattgctg ccgatagtaa aagtaaccat caaattgctt tgcaacaatt
      121 gcgtggaggt tttagcagtg atattaaaaa actgcgcgaa tcattaattc attttgcatc
      181 gcttattgaa ttggaattgg attttgccga agaggatgta acttttgcca gccgccagca
      241 attaactcag ttggttagta acatgcacca aaccattgat gctttggcgc aatcgttcga
      301 aataggtaat gtgatgaagc atggcatacc cgttgtaatt gcaggcatgc ccaatgtggg
      361 caaatcaacc ttactaaacg ccttacttaa tgaagagcgt gcaattgtta gcgaaattgc
      421 cggcaccaca cgcgatacaa ttgaagagca aattaatatt gaaggtttaa atttcagatt
      481 tacggataca gcaggcatca gacaaacggt tgatgcaatt gaagcattgg gcgtggagcg
      541 cacttatcaa aaactgaagt taagtgctgt tgtaatttac atgtttgacc ctaagcatgt
      601 tactccggtt gaggttctga aaactctgga tgaattgaaa agtaacctgt gttgaataaa
      661 accgacacca tacctgagtc tgaacttgtt ccttttaatc agatagccga aataagtttt
      721 atttcggcca aacaaaaatg gcaaattgaa aagctcattc aattattatt aaacaaagtg
      781 caacatgtta aagtaagttt agattcgaca atcgttgtaa atgtgcgcca caaagaggca
      841 ttggataata ccagccaagc cttacaaaaa gtgcttcatg gtattgaaac ccaaatatcg
      901 ggcgattttt tagccagcga cattagagaa gcactttatc acctcggact tattaccggt
      961 gagattgcca ccaacgacct gcttgaaaac atctttagtc gcttttgcat cggcaaataa
     1021 aagtacaccg tctgttgtat taaatatcct cttaactctc aaataatcac aattttcaaa
     1081 attgaaaaca caatttgatt atgaaaaaaa aatcaattga tgttggctca acatggaaat
     1141 taaacgtaca gcattatcaa ggataaatta tacaactttc gttgtaatat cggttcgttt
     1201 caaaatcaaa ataatacctt agccatgcta tgaaatcttt tatttgggct tttgcctttt
     1261 cgatatttcc aaccatatta tacgcacaag tgggatctat aattaatctg ggtgtggtta
     1321 aatctaaata tgcatcaggc tataatgttg atgtttggct acctgcaaac tacagtgcat
     1381 cgaagcaata tgcagtaata tacatgcacg atgggcagat gctttttgat agtacaacca
     1441 cgtggaataa acaagcatgg atggtacaaa atgtggttgc taatttaatg gctgaaaata
     1501 aaatagtgcc agcaataatt attgccatac ataataacgg acaaagccgc catgcagatt
     1561 atacaccaca aatacctttt aaaaaattaa agcggcaaca gcaaactgca atgtatcatg
     1621 atacaactgc ttatggcaat aatgtgtttg caaattttaa agtaaacagc gataactatc
     1681 ttaagttttt agtgaatgag ttaaaaccac ttattgacaa acgatttagc acctataccg
     1741 acaagagcca aaccattatt atgggaagca gcatgggtgg tttgatttca ttatatgcca
     1801 tttgtcaata tcccgaaatt tttggtaagg cagcatgcat gtcaacccat tggccattgg
     1861 tttttaaaac cgacaacaat ccttttccgg atgcaatgct ccaatattta attggcaaat
     1921 taccacaacc cgaaagccac atgctttatt tcgatatggg caccatgggt atcgactcat
     1981 tgtacgaaag cacacaaatt caggttgatg ctttatgtat tgatataggt tataacagca
     2041 ataattttat gtcagttgtt tttgaaggtg ccgatcataa tgaaaaagca tggcataaac
     2101 gtttgcatat tccgctgcag tttttgctca aaccttaatt tgtttttatc ctatatagta
     2161 acattaattc attaagcact atgcattatc catatcaaat aaaatcgcaa cagcaatatg
     2221 aaaacgatta tgccctgagc attaaaaatc caaaggagtt ttgggcaaat gttgcgcaac
     2281 attttacctg gtttaagcca tacgatacgg ttttaaactg ggagtttaat acacctgatg
     2341 taaaatggtt cgaaggtggt aaactgaaca ttacttataa ttgtttagac aggcatgcgg
     2401 ctacacatcc cgataaggtt gcatttattt ttgaacctaa cgatattaat caacgcacac
     2461 aaaaaataac gtatgcgcaa ttgttgcaac aggttagcca aacttgttat gcattacaaa
     2521 gcataggcgt acaaaaagga agccgcgtaa ttatttacat gggtatggtg ccacaattgg
     2581 caataacttt actggcatgt gcgcgtatgg gtgccgtgca cagtgtggta tttggagggt
     2641 tttcggc
//
LOCUS       DAOMAA010000195         2645 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_33251_length_2645_cov_3.379537, whole genome shotgun sequence.
ACCESSION   DAOMAA010000195 DAOMAA010000000
VERSION     DAOMAA010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33251_length_2645_cov_3.379537"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PLO59_03510"
     CDS             complement(<1..194)
                     /locus_tag="PLO59_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HQV00199.1"
                     /translation="MKKLILITGIATLIYACSGSSENKTSDTAAAETATATETPEEKQ
                     AADARGIGKFTNVEVGETLDA"
     gene            complement(459..869)
                     /locus_tag="PLO59_03515"
     CDS             complement(459..869)
                     /locus_tag="PLO59_03515"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HQV00200.1"
                     /translation="MKKKSFETFCTQYKNSQFFTPANPLRLIGDNLMLKEFFTYSKNE
                     FIYRIGEPATGVFCIYSGDIKIFKPDESSKGLNIHRAKAGEIFGFNSIANGKFTNSAK
                     AMETSDICFVPLTEMHRLIRFISSTTELILSSKC"
     gene            complement(988..1698)
                     /locus_tag="PLO59_03520"
     CDS             complement(988..1698)
                     /locus_tag="PLO59_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HQV00201.1"
                     /translation="MNKNNPHPECHQCQVRFKSVFCDLGKEDLDTINNNKGCNTYQKG
                     QIIFYEDAIPHGLFCINNGKVKVSQAGEAGKEQIVRLAKEGDILGYRALLSGEKYSCS
                     ATALDTTSICFIPKQTFFGLIDKNANLSKEILKLLSHDLRKAEHKITDLAQKPVRERL
                     AEALLFLKETYGLEPDDATLDVVLSREEIANIVGTATETTIRLLSEFKQDKMIDLKGK
                     KITFLNLPSLIKTANIND"
     gene            complement(1816..2142)
                     /locus_tag="PLO59_03525"
     CDS             complement(1816..2142)
                     /locus_tag="PLO59_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004920112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HQV00202.1"
                     /translation="MNWILLIIAGLFEALFAFCLGKAKTTSGQLMYLWYAGFLLALVI
                     SMLLLIKATQTLPIGTAYAVWTGIGAIGTVILGILIFNEPVTALRLLFITTLILSIVG
                     LKIVSS"
     gene            complement(2173..>2645)
                     /locus_tag="PLO59_03530"
     CDS             complement(2173..>2645)
                     /locus_tag="PLO59_03530"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HQV00203.1"
                     /translation="LNKIAFGQANEAMLVDALRRNSNVFIPELSIVALYKDAIVGHIL
                     FTKVWITDHQGNKYQSLSLAPMAVLPAYQNQGIGGQLINKGLSVAAALGHKSVIVLGH
                     EHYYPKFGFKPANIWHIKAPFDVPSKNFMAIELVTNGLAQINGTVFYPPEFDAV"
BASE COUNT          778 a          452 c          480 g          935 t
ORIGIN      
        1 gcatctaatg tttcgcctac ttcaacattg gtaaacttgc ctatgccgcg tgcatcagca
       61 gcttgttttt cttcaggtgt ttcggtagct gttgccgttt cagctgctgc tgtgtcagat
      121 gttttatttt ctgatgaccc tgaacatgca taaataagtg tagcaatacc ggtaattaaa
      181 attagttttt tcattagtta attgttttga gtttaagttg aattatattt aaaaaaattg
      241 tggcgttgtt gaaatagata agtgttctaa attgagcttc tattctacat atcatttggt
      301 atggcttcaa gctgcagtat tttatccttt ttatacattt caaaataccc gaaatagttg
      361 tttgtggtta agttttaatt taatggtaca aagttatttt gagtaattat ttgattgaat
      421 gatgcggctt atacttactt atgatttata tcatgttttt agcatttgct gcttaatata
      481 agttctgttg ttgaggatat aaatcgtatt aagcgatgca tttctgttag tggtacaaag
      541 caaatatcag aagtttccat ggctttggcc gaattggtaa attttccgtt agctattgaa
      601 ttaaagccga atatctcgcc agcttttgct ctgtgtatat ttaaaccctt tgacgattca
      661 tccggtttga aaatttttat atcaccggaa taaatgcaga aaacaccggt ggccggttcg
      721 ccaatacgat agataaattc gtttttactg tacgtaaaaa attctttcag catgaggttg
      781 tcgcctatca accgtaatgg atttgcaggt gtaaagaatt gtgagttttt gtactgggta
      841 caaaatgttt caaaagattt ttttttcatc gcttgtttgc aaagacttag tttatcctat
      901 gcaaaggacg cagtcatggt ttggaagtaa atgaattaca tcaattagaa atatgattta
      961 aatcatactt taattgccgc ccaccagtta gtcgtttatg ttggcagttt ttattaatga
     1021 aggtaaatta agaaaagtaa tctttttgcc tttaaggtca atcattttat cctgcttaaa
     1081 ttcagagagc aaacgtatgg ttgtttcggt tgcagtacct acaatgttgg caatttcctc
     1141 gcgcgataat actacatcca gtgtggcatc gtccggctct aatccatacg tttcttttaa
     1201 aaaaagtaag gcttcggcaa gtctttcgcg tacaggtttt tgagccaaat ctgtaatttt
     1261 atgttcggct tttcttaaat cgtgtgatag cagctttaga atttctttcg ataagttggc
     1321 gttcttgtca attaaaccaa aaaatgtttg tttcggaata aaacaaatag atgttgtatc
     1381 taatgccgtt gccgagcatg aatatttttc gccactaagt aaggcgcgat aacctaaaat
     1441 atcgccctct tttgctaaac gtacaatttg ttctttgccg gcttcgcctg cttgcgaaac
     1501 ttttacttta ccgttgttta tgcaaaacaa accatgcgga atggcatctt cataaaaaat
     1561 gatttggccc ttttgatagg tattacaacc tttattattg ttgattgtat ccaaatcctc
     1621 tttgcccaaa tcgcaaaata ctgacttgaa tcgaacttga cattgatgac attcgggatg
     1681 tgggttgttc ttattcattt gtatgtaatg cagatgtgcg aatgtagtac tttgatacta
     1741 acattgttgc aataagttat agtcttattt ttaacaattt gcaacgtttc attgtatgct
     1801 ttttggcgct gtagttcagc ttgaaactat ttttaaaccc acaatggata gtatcaatgt
     1861 agttataaaa agcagcctca aagcggtaac cggttcgttg aaaattaata tgcccaaaat
     1921 aacagtgcct atggcaccaa tgcccgtcca aactgcataa gctgtaccta taggtaatgt
     1981 ttgtgtggct tttattagta aaagcatact tataaccaat gccaataaaa aaccggcata
     2041 ccataaatac attaattggc ctgaagttgt ttttgctttg cccaaacaaa atgcaaacaa
     2101 ggcttcaaat aagcctgcta taattaataa aatccagttc atgtataaat gtttatgtag
     2161 atacaataca gtttaaacag catcaaattc gggtgggtaa aaaacggtgc cgtttatttg
     2221 tgccaatcca tttgtaacaa gttctattgc cataaagttt tttgaaggta catcaaatgg
     2281 agctttaata tgccagatat tggcaggttt aaatccaaat ttagggtagt aatgttcatg
     2341 ccctaaaaca attaccgact tatggcctaa ggcagccgca accgataagc ctttgttaat
     2401 tagttgtccg cctatacctt ggttttgata tgcaggcaat acagccattg gtgccaaact
     2461 taagctttga tacttattgc cttgatggtc tgttatccac actttggtaa ataaaatatg
     2521 acctacaatt gcatccttat ataatgccac tatggaaagc tcgggaataa acacatttga
     2581 atttcgtctt aatgcatcaa ccagcatagc ttcattggcc tgaccaaaag caattttatt
     2641 taatt
//
LOCUS       DAOMAA010000196         2644 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_33290_length_2644_cov_2.866744, whole genome shotgun sequence.
ACCESSION   DAOMAA010000196 DAOMAA010000000
VERSION     DAOMAA010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33290_length_2644_cov_2.866744"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /locus_tag="PLO59_03535"
     CDS             complement(<1..829)
                     /locus_tag="PLO59_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HQV00204.1"
                     /translation="MLANKYDKTTLKARLDKEDFKRTTLSFYRYTQLEPETFRDVLYK
                     SFDALQIFGRVYVAQEGINAQISVPNHHFEQFKELLQSVAALKNMPLKIAVADDCKSF
                     YKLIVRVKHKIVADGLGEIDFSDVGNHINAAELNKALDNPETVIIDMRNHYESEIGHF
                     EGALCPDADTFREALPQAIAMAADKKDKKIILYCTGGIRCEKAGAYFKQHGFTDVNQL
                     HGGIIAYAAQIKEQGLTSKFIGKNFVFDDRLGERITEDVISKCHQCGQPCDTHTNCKN
                     "
     gene            complement(886..1113)
                     /locus_tag="PLO59_03540"
     CDS             complement(886..1113)
                     /locus_tag="PLO59_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00205.1"
                     /translation="MKKWLIIFLLIVVAYKSPAQCSMCKATVESNMNDNADFVGKGLN
                     KGILYLMSIPYLMGGVAYIIYRVYRKPKATA"
     gene            complement(1207..1677)
                     /locus_tag="PLO59_03545"
     CDS             complement(1207..1677)
                     /locus_tag="PLO59_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008239788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HQV00206.1"
                     /translation="MLLLNRKVLPRPEPTPDFVYILPAINAIINATCTILLLLSYYFI
                     RQKNVTMHKRLNITTFVLSALFFICYITYHYMADETRFPVGAPYRSFYLTILASHIVL
                     AALVLPMILISFYLGLTQKVALHKKWVRFTFPIWLYVTVTGVIVYLMLSPYYNH"
     gene            complement(1783..2379)
                     /locus_tag="PLO59_03550"
     CDS             complement(1783..2379)
                     /locus_tag="PLO59_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00207.1"
                     /translation="MLLGPALFYFLLTRGKNTYKSLEIYGPVRFENGARGIDTIYHIA
                     ADFNLSDQKGNRVSLSAFPNKIYVVSIHQSNNTTGTALAKIQGIVYQYKADTNLVFVS
                     ILKNQSLTADSLALKYRAKYGKWLFAEADSTINNDFFKSYFVSNSDSINTSSNVVLLD
                     ETKRVRGFYDATDAWDIAKLGDEIKVLEFERFGKVFRD"
     gene            complement(2461..>2644)
                     /locus_tag="PLO59_03555"
     CDS             complement(2461..>2644)
                     /locus_tag="PLO59_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008611990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HQV00208.1"
                     /translation="YIFIALTIVKAYFIVYFFMHLKAEWKSFQYTIILPFILIVYLIF
                     IALIEGNYLTKVMTGN"
BASE COUNT          866 a          472 c          467 g          839 t
ORIGIN      
        1 catttttgca gtttgtgtgc gtatcgcatg gttggccaca ttgatgacat ttactaatta
       61 catcttcagt aatacgctcg ccaagtctgt catcaaacac aaagttttta ccaatgaatt
      121 ttgatgtaag accttgttct tttatttgcg cagcgtatgc aattatgcca ccatgtaatt
      181 ggtttacatc tgtaaaccca tgttgcttaa agtaagcacc ggctttctcg cacctgatac
      241 cgcctgtaca atacagtata atcttcttat cttttttatc ggcagccatg gctattgctt
      301 gcggcaatgc ttctctaaaa gtatcagcat cggggcataa tgcgccttca aaatgaccta
      361 tttcactttc ataatgattt cgcatatcaa ttataactgt ttccggattg tctaaggctt
      421 tatttagttc ggcagcgtta atgtgattgc caacatcact aaaatctatt tcgcccaaac
      481 catcagcaac aattttatgt tttacccgaa caattaattt gtaaaacgat ttgcaatcat
      541 ccgctacagc aattttaagc ggcatatttt ttaatgcagc tactgattgc agtagttcct
      601 taaattgttc gaaatggtga ttgggaacac ttatttgggc gttaattcct tcttgtgcta
      661 catatacacg cccaaatatc tgaagtgcat caaaactttt ataaagtaca tccctaaaag
      721 tttcgggttc taattgtgta tagcgataaa atgaaagtgt agtgcgctta aaatcttctt
      781 tatcaagtcg tgcttttaag gtagtcttat cgtatttgtt tgctaacata gtgatgatat
      841 gcgtattggc agtaatacat tttttagtaa agcttaattt gcaatttatg cagtagcttt
      901 tggtttacgg taaacacggt aaataatata agccacaccg cccattaagt atgggattga
      961 cattaaatat agaatgcctt tgtttaaacc tttaccaaca aaatcggcat tgtcgttcat
     1021 gttcgattca acggttgctt tacacatact gcattgcgca ggcgatttat aagccacaac
     1081 tatcagtaaa aaaataatta gccatttttt catgtgtgca aaagtaaaaa actacataat
     1141 cgtacaagtg atttaacgtg caataacgtt agcttgtggc taacaaatta tgacttaaat
     1201 cataacctaa tgattatagt aaggcgataa cattaaataa acaattacac ctgttacggt
     1261 tacatataac cagataggga aggtaaaacg aacccacttt ttatgcaaag caactttttg
     1321 agttaagcct aaataaaaag aaattaaaat catgggtagt accaatgcag ccaaaacaat
     1381 atgtgatgca aggatggtta aatagaatga acggtaaggt gcaccaaccg gaaacctggt
     1441 ttcatcggcc atgtagtgat aggtgatgta acaaatgaaa aacagtgccg acaatacaaa
     1501 cgtggtaatg tttaaccgtt tatgcatggt aacatttttt tgccgtataa aataatatga
     1561 cagcaatagc aaaatggtgc aggttgcatt tataatggca tttattgcag gtagtatata
     1621 cacaaagtcg ggtgtgggtt cggggcgggg cagtaccttg cggtttaaca ataacacggc
     1681 cgcaaaaaca aaaatggaaa tacaggctac ccacttaaaa ataagtttat cctgcttcgt
     1741 atagttttgg ctactcattt attctgaatg gatttgatat acttaatccc gaaaaacttt
     1801 tccaaatcgt tcaaattcta aaactttaat ttcatcgcca agtttggcaa tatcccaagc
     1861 atcagttgca tcataaaagc cacgcactct tttggtttca tctaacaaaa ccacattgga
     1921 cgatgtgttt atgctatcgc tatttgaaac aaagtatgat ttaaaaaagt cattattaat
     1981 ggttgagtcg gcttcggcaa aaagccattt gccatacttt gcacggtatt tcaaagccaa
     2041 actatctgca gttaatgatt ggttttttaa aatagaaaca aataccaagt tggtatccgc
     2101 cttatattga taaacaatgc cttgtatttt agctaaagct gtaccggtag tgttgtttga
     2161 ttggtgtata gaaactacat agattttgtt tggaaaagcg gagagcgaaa cccgattgcc
     2221 tttttgatca gataaattaa aatcggcagc aatatgatat atggtatcaa taccgcgcgc
     2281 cccattttcg aaacgcaccg gcccataaat ttctaatgat ttatatgtgt ttttgccccg
     2341 ggttagcaaa aaataaaaca gggcaggccc cagtaacaca agtaatgctg ttataattag
     2401 cctaaccttt gtgctacgct tttgttgcat ataaagttaa agtaggattg tagaaaagta
     2461 ttaattacct gtcattactt ttgttaagta attgccttca atcagcgcaa taaaaatcaa
     2521 ataaacaata agtataaaag gcaaaattat ggtatattga aacgatttcc actcagcctt
     2581 taaatgcata aaaaagtaaa ctataaagta agccttaact atagtaagcg ctataaagat
     2641 atag
//
LOCUS       DAOMAA010000197         2636 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_33484_length_2636_cov_2.469585, whole genome shotgun sequence.
ACCESSION   DAOMAA010000197 DAOMAA010000000
VERSION     DAOMAA010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33484_length_2636_cov_2.469585"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..>2636
                     /locus_tag="PLO59_03560"
     CDS             145..>2636
                     /locus_tag="PLO59_03560"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HQV00209.1"
                     /translation="MKALKITGLILAIVICAIILIPIIFKNKIAEAAKTQINQSLLAT
                     VNFSDFDLHLIRSFPNLTFQINDLSVVGTDVFKNDTIIYAQTLAVKLSIWEVLCSSNY
                     KIQSFELENALLNIKILPNGQANYNIVKPDSSSASESDASAFKLALKKYSINHSNINY
                     NDAQSHIYVSIENVNHKGSGDFTQDVVNLITQTEIEKMSVTMDKINYLNRVKTKAAAT
                     FNIDMKAGKYTFVKNSISLNDLMMHFDGSLTLAENNIYADLTWGIDKNDLKSFMSLIP
                     NVYQNNFSNLTCSGNLAAKGFIKGNYNANQIPAFDFSLKIDNGRFKYSQLPNEVKNIN
                     VDFVAINNSGIENETIINLKKFEANLGGDAISARLKITTPVLQPYLDAALKGTINLSN
                     FKSFMPLAKDESLSGIVSADITANGYYTDIEKSAYQKFNAAGYIQVNDLKYNSQALTQ
                     ATTINRLKLVFNPQTAILEYADMQFGESDFKAQGQLSNFWGYYLNNQMLSGKLQVQSQ
                     KINLNQFAGNTANDTATQSSTGIIEVPANLHFELLANAGNCTYNQINISDFAGSIKIA
                     NQTVQLNQASFKTLDGVVNMSGSYSTQNIDAPNISYKLDLKQIDIKAACKTFNEIETM
                     APIAAYVHGKVSSQFNVIGALDANMQPVLKTLTGWGSLSTHQLGTSNMPLLNEVANSL
                     KMPVLNNPTIGDVTLAFKFVDGRLLVDPYSMKLNDYKASISGSNGFDKTINYFLKLEV
                     PKSKIPASAMATVTNLLQKANAQLNTQLTLPDPVPIDIEIIGNILKPQIKTQLAQTGK
                     NMATTLTDAATTKAKEEARKQADKLLAEAELKA"
BASE COUNT          972 a          496 c          453 g          715 t
ORIGIN      
        1 aaaattcgaa ataacaaatg cacaaataaa aaacctttgt gttatcgaat taggtgcgcg
       61 caatgcaact ttataattgt cgttatttgt gctatggcaa taggcacggt ttttaatata
      121 gatgcctaaa cacaatacaa cattatgaaa gctttaaaaa ttacaggttt aattttagcc
      181 atcgttatat gcgcaataat tctaataccc atcattttta aaaataaaat agccgaagcg
      241 gcaaaaacac agataaacca atcgttattg gcaacggtaa atttcagtga ttttgattta
      301 catctgattc gcagttttcc gaaccttact tttcaaataa atgatttaag tgttgttggt
      361 acagatgtgt ttaaaaacga cacaataatt tatgcgcaaa ctctggctgt aaaacttagt
      421 atatgggagg tgttatgcag cagcaattat aaaattcaat cgttcgaatt agaaaatgca
      481 ttgcttaata ttaaaatttt accaaacggg caagccaatt ataacatagt aaaaccagat
      541 agcagcagtg cttccgaaag tgatgcatca gcatttaagc tggcattaaa aaagtacagc
      601 atcaaccact cgaatattaa ctataacgat gcacaaagcc atatttatgt ttcaattgaa
      661 aatgtaaacc ataaaggcag tggcgatttt acacaggacg ttgtaaacct gattacacaa
      721 accgaaatag aaaaaatgtc ggttacaatg gacaaaataa attatctgaa ccgcgtaaaa
      781 accaaagccg ctgccacttt taatattgat atgaaagccg gtaaatacac ttttgtaaaa
      841 aacagcatat cgcttaacga tttaatgatg cattttgatg gatccctgac gctggcagaa
      901 aataatattt atgctgattt aacctggggt atagataaaa acgacttaaa aagttttatg
      961 tcgcttatcc ccaatgtgta tcaaaacaat tttagcaacc ttacttgcag cggtaatttg
     1021 gctgcaaaag gttttataaa aggaaactat aacgccaatc aaatacctgc attcgacttc
     1081 agtttaaaaa ttgataatgg ccgttttaaa tacagtcaat tacccaatga agtaaaaaat
     1141 ataaacgttg attttgtggc cattaacaat agtggtattg aaaacgaaac cattattaat
     1201 ttaaaaaagt ttgaagccaa tttaggtggc gatgccattt cagcccgatt aaaaataact
     1261 acacctgttt tacaacccta cctcgatgct gccttaaaag gaactattaa cctttctaat
     1321 tttaaaagct ttatgccttt ggcaaaagat gaatcattaa gcggcatcgt gtcggccgat
     1381 ataactgcca atggatatta tacggacata gaaaaaagcg cctatcaaaa atttaatgca
     1441 gccggttaca tacaggtaaa cgatttaaaa tataacagtc aagctttaac acaggcaact
     1501 acaataaacc ggttaaagct ggtgtttaat ccgcaaacgg ccatacttga atatgccgat
     1561 atgcagtttg gtgaaagcga ttttaaagcg caggggcaac tcagcaactt ttggggttat
     1621 tacttaaaca accaaatgtt atcaggcaaa ttacaagtgc aatcacaaaa aataaatctt
     1681 aaccagtttg ccggcaatac agcgaacgat acggctacac aaagcagcac aggtattatt
     1741 gaagtacctg caaatttaca tttcgaactt ttggcaaatg ccggtaattg cacctataat
     1801 caaataaaca tcagcgactt tgcaggcagt attaaaattg caaaccaaac agtgcagtta
     1861 aatcaagcaa gttttaaaac attggatggt gttgtaaaca tgtcgggcag ctattcaact
     1921 caaaacatag acgcaccaaa catcagttat aaactcgact taaaacagat agatataaaa
     1981 gcagcttgta aaacatttaa cgaaattgaa accatggcac ctattgctgc ttatgtgcac
     2041 ggcaaagtat catcacagtt taatgttata ggcgcattgg atgccaacat gcaacccgtg
     2101 cttaaaacac ttaccggttg gggttcgtta agcacacatc agttgggtac ttcaaacatg
     2161 ccactattaa atgaagtggc caattcatta aaaatgcctg ttttaaataa cccaacaata
     2221 ggtgatgtaa cgcttgcttt taaatttgta gatggccgct tactggttga cccatacagt
     2281 atgaaactta acgactacaa agccagcatt agtggtagca atgggtttga taaaaccatt
     2341 aattattttt taaaactaga agtacctaaa tcaaaaattc cggcttcggc aatggcaacc
     2401 gtaactaatt tgctccaaaa agccaatgca cagttaaaca cacaactaac gttgcccgat
     2461 ccggtgccca ttgatatcga aattattggc aacattttaa aaccgcagat aaaaacacaa
     2521 cttgcgcaaa cgggcaaaaa catggcaact acccttaccg atgctgctac caccaaagca
     2581 aaagaagaag cacgcaaaca agcagacaaa ctattggccg aggccgaatt aaaggc
//
LOCUS       DAOMAA010000198         2635 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_33504_length_2635_cov_2.181008, whole genome shotgun sequence.
ACCESSION   DAOMAA010000198 DAOMAA010000000
VERSION     DAOMAA010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33504_length_2635_cov_2.181008"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="PLO59_03565"
     CDS             <1..481
                     /locus_tag="PLO59_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="organic solvent tolerance protein OstA"
                     /protein_id="HQV00210.1"
                     /translation="ADSMMRFYNQPILWSNQSQLTATHIYMQLSNNKISTMHLLQSAF
                     IINVSDSLQFNQIRGKDMVGYFLDNKLRKIDVNGNGQTVYYPKNKDDKTIGANRAECT
                     DLVIYIDSNQIQSITFINKPDGTMFPIKDVKPVDFTLKGFKWLIQLQPKRKDDIYLK"
     gene            708..929
                     /locus_tag="PLO59_03570"
     CDS             708..929
                     /locus_tag="PLO59_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00211.1"
                     /translation="MLKNRMYCVLIWVFIANIAKAQSYRYLPADSARIAIATATTPEE
                     KFYGYYSLDRYYYTSGLYDSSEIVQREMY"
     gene            960..2099
                     /locus_tag="PLO59_03575"
     CDS             960..2099
                     /locus_tag="PLO59_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00212.1"
                     /translation="MVDINLAISNKYINKQEINLALIYALKALDYAHQDFRKARTYIN
                     IAGVYAWNANYITALQYLRKYDDLGSYPHFTLFRNMMYGMAFNGLAEPDSALVYLLST
                     ETIFKKRPDVFGYAQALNQFAKAYEIKGDSDLAIAYYKKTLAFCEQNGVGSFYIIACN
                     NYCKLLISMQNYNEAKRLAHANLITATRGGNFYGVSLAAQVLQQVYGLHNTDSAYHFA
                     LMQIAYQDSANSLKKIHEFQNITFAQQLREIDEQAKSEVAALQRKQNIQYGLIALCIV
                     SLIFMFLILSRTIITNPKIIRFISALALLIVFEFLNLLLHPLLEKITHHTPVLMLLGL
                     VCIAFLLVPMHHKIEKWATDKLIEKNKQIRLAQARKTIVDIETEN"
     gene            complement(2340..>2635)
                     /locus_tag="PLO59_03580"
     CDS             complement(2340..>2635)
                     /locus_tag="PLO59_03580"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV00213.1"
                     /translation="GAYESVTGIEAVNQAANLTVSPNPGNGLFGLKLTGAINGVYETQ
                     VFDATGKMIYSYHIENTSSLLLHTFDLQNQPKGIYTLKLINNNKVLTHKVVKL"
BASE COUNT          895 a          432 c          443 g          865 t
ORIGIN      
        1 agccgatagt atgatgcgtt tttataatca acctatatta tggagtaacc aatcgcaact
       61 tacggcaacg catatttata tgcagctatc aaacaataag ataagtacca tgcatttatt
      121 gcaaagtgca tttattataa acgtaagcga ttcgttacag tttaatcaaa taagaggcaa
      181 agatatggta ggctattttt tggataataa gcttcgtaaa attgatgtaa acggtaacgg
      241 acaaacggtt tattatccta aaaataaaga cgacaaaacc attggtgcta accgtgctga
      301 gtgtacagac ctggttattt atattgatag caaccaaatt caaagtataa cgtttatcaa
      361 taagcccgat ggcactatgt ttccgataaa agatgtaaag cctgttgact ttacgctcaa
      421 aggatttaaa tggttgatac aattacaacc aaaacgaaag gatgatattt atttgaagta
      481 aatcactttt ataacttcaa aattgaacta acaattgttc gcatagtgca tttgcatcta
      541 tgcctctaaa atgttgcttt tataatttgc aaccacgcaa accagcgact gatgtttggc
      601 ttaaattaaa aatagcagct tctatatttt gttatttaat taaatcaatt accgatattt
      661 attatttcgc ttttttgcat tattaaaagc tgattaagta agttgctatg ttgaagaata
      721 gaatgtattg cgtattaata tgggttttta tagcaaatat tgctaaggca caatcttatc
      781 gctatttgcc ggccgactcg gcacgtatag ctattgcaac tgcaacaaca cctgaagaaa
      841 aattttacgg atactatagc ctcgaccgat attattatac ctcgggtttg tacgatagtt
      901 cagaaattgt acaaagagag atgtattaaa tttcgaaaaa actaaataac gattcgttat
      961 tggttgatat caacttggca ataagtaata aatacataaa taaacaagaa ataaatctgg
     1021 cacttattta tgcgctaaaa gcattggatt atgcgcatca ggattttaga aaagccagaa
     1081 cctacataaa tattgcaggt gtttatgcat ggaatgccaa ttatataact gccttgcaat
     1141 accttagaaa gtatgatgat ttaggcagct acccacattt taccttgttt agaaatatga
     1201 tgtatggcat ggcatttaat ggcttggcag agcccgattc ggctcttgtt tatttgcttt
     1261 caacagaaac tatttttaaa aaaaggcctg atgtttttgg atatgcacaa gcactaaatc
     1321 aatttgcaaa agcgtatgaa ataaaaggcg atagtgattt agccattgct tactataaaa
     1381 aaacacttgc attttgcgaa caaaatggtg taggctcatt ttatattatt gcttgtaaca
     1441 attattgtaa gcttttaata agcatgcaaa actataacga agccaaacgc ctggctcatg
     1501 ccaatttaat taccgctacg cgcggtggaa acttttatgg tgtatccttg gctgcgcaag
     1561 ttttacaaca ggtatatggc ttacataaca ctgatagtgc ctatcatttt gctctaatgc
     1621 aaattgcata tcaggattcg gctaacagcc tgaaaaaaat acatgaattt caaaacataa
     1681 cctttgcaca gcaattgcgc gaaattgatg aacaagccaa atcagaagtg gcagctctgc
     1741 aacgcaaaca aaatatacaa tatggcttaa ttgctttatg catcgttagt ttaatattta
     1801 tgtttttaat cttaagccgt acaatcatta ccaatccaaa aataattcgg tttataagtg
     1861 cattagcact attaattgtg ttcgaatttc ttaacttgtt actacaccca ttactggaaa
     1921 aaataacaca tcacacaccg gtacttatgc tactgggttt ggtgtgtata gcttttttat
     1981 tagtaccaat gcatcataaa atcgaaaagt gggctactga taaacttatt gaaaagaaca
     2041 aacaaatccg tttagcacag gcaagaaaaa ccatagtcga tattgaaacg gaaaattaac
     2101 gcaccgcttc atatttttta gttttaaatt tcaattttgg atgctgcatc gcaaatttat
     2161 tgctgttttg cctgtatgtg tgataaataa tgctatccgg ttttattagg taataggcaa
     2221 cacttaaaaa ttaaaattga gcttaaacaa gttctttact tctattgatt ctgttttaaa
     2281 aataaaaaag ctgcttagaa atatatttcc aagcagcttt tttattatcg gatgtaaact
     2341 taaagtttaa ctactttgtg ggtaagtact ttgttattat ttataagttt taaagtataa
     2401 atccctttag gttgattttg taaatcaaac gtatgcagta gtaaactact tgtattttca
     2461 atgtggtagc tgtaaatcat tttgccggtt gcatcaaata cttgtgtttc ataaactccg
     2521 tttatggcac cggttagttt taaaccaaac aaaccattac cggggtttgg gcttacagtt
     2581 aaatttgccg cttggtttac agcttcaata cccgtaaccg attcataagc gccaa
//
LOCUS       DAOMAA010000199         2634 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_33522_length_2634_cov_3.181078, whole genome shotgun sequence.
ACCESSION   DAOMAA010000199 DAOMAA010000000
VERSION     DAOMAA010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33522_length_2634_cov_3.181078"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="PLO59_03585"
     CDS             <1..292
                     /locus_tag="PLO59_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00214.1"
                     /translation="MTTNFYFKPTITTAEMRNINNALRKNRRILISLSPDGKAKVSKQ
                     KLLDNGFNFKYHTHTHTTQKGNVYKLCYDYGLTPLEQDFFMIIKWGVFNQNT"
     gene            complement(298..2058)
                     /locus_tag="PLO59_03590"
     CDS             complement(298..2058)
                     /locus_tag="PLO59_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQV00215.1"
                     /translation="MAICLLVLVSYGAAVFNGYALDDFIVLAKNKFVQQGFAGIASIF
                     GNDTFAGLSDANTMVLTGGRYRPLSVVSFAMEYQLWGLAPAISHAINVLLYACSCCLL
                     YRCLQYAWPFKAFTLSVTLLFAALPIHSEVIINIKGRDDIFCLLFFLSSILILLKNKN
                     TLSSFVLLQAAVLYFLALLSKETAITFLAIYPFVFAFTMHIKKALINSCYMLIPVLLF
                     LLIRQMAVGGNTGAISTDVFNNPFIHADRLQRLATVLLTWLLYLQKIVWPSNLAYDYS
                     FNQIPITDFGNLWVWLSLFIHAGITVWAILQFKTQKWIAFAWLWYVATFSIVSNLLFN
                     IGAPLADRFMYIPSLGIILLIVLCLNALFKNIKSVNEKLSVGITIMLLLPMIWHNYSR
                     CANWKNNHALFLHDVFVVPQNAKAQLNAGLSYIEIAQQDTVKPQLPVIQKAIAHLQNG
                     IKIYPQFIDGYLNMGVAYNTIGNIDSALWWWDKARTIDAYNSSLKTYDGIMAGYFLQK
                     GLQAGTTQNFGVAVTNLKKSVALDSTNAEAWYNLGGANFSMGMYNDAVIAWQKTITIN
                     PGHAQAAAGLQAALQKVKQP"
     gene            complement(2179..>2634)
                     /locus_tag="PLO59_03595"
     CDS             complement(2179..>2634)
                     /locus_tag="PLO59_03595"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQV00216.1"
                     /translation="RENETLKNRIASVETVSKNKKTPQAAVAQSGLEASFKYDIYDSA
                     VSLYNTAQYKESLAKFETILQHNSTNSNSNFYAGMCYYHLGNYTMAIRYYEQAGIDKK
                     ALLYEDALYYKALSLKALGQTNEAKSTLQKVIKLKSTHQHQAKKLLQSW"
BASE COUNT          837 a          498 c          483 g          816 t
ORIGIN      
        1 aatgacaacc aatttttatt tcaagccaac tattactact gccgaaatgc gcaacatcaa
       61 taatgcatta cgcaaaaaca ggcgcatact gataagcctg tcccctgatg gcaaagccaa
      121 ggtgagcaag caaaagttgt tagacaatgg gtttaatttt aaataccata cacatacaca
      181 caccacccaa aagggtaatg tatataagct ttgttacgat tatggattaa cacccttgga
      241 acaagatttt tttatgatta ttaaatgggg tgtatttaac caaaatacgt aatggcttta
      301 cggttgtttt actttttgta aagcagcttg taaacccgct gctgcctgtg catgccccgg
      361 attaatagtt atggtttttt gccaggcaat aacagcatcg ttatacatac ccatactaaa
      421 attagcacca cccaaattgt accaggcctc ggcattggta ctatctaatg caaccgattt
      481 ttttaagttg gttaccgcta cgccaaagtt ttgcgtagta ccggcttgta accctttttg
      541 taagaaatac ccggccatta taccatcata agtttttagc gaactgttgt atgcatcaat
      601 agtacgggct ttatcccacc accaaagcgc actatcaata ttgccaatgg tattataagc
      661 tacgcccatg tttaaatagc catcaataaa ctgcggatat attttaatgc cgttttgcaa
      721 gtgagcgatg gctttttgta taacaggtaa ctgtggctta acggtatctt gttgtgctat
      781 ttcaatataa gataagcctg cattaagttg tgctttggca ttttgcggta ctacaaatac
      841 atcgtgtaaa aataatgcat gattgttttt ccaattggcg catcggctgt aattgtgcca
      901 tatcatgggc agtaaaagca tgatggttat accaacgctt agtttttcgt ttacggattt
      961 tatgttttta aacaaggcgt taagacaaag aacaattaat aaaattattc ccaatgaagg
     1021 gatatacata aaccgatcgg ccagaggtgc acctatgtta aacagcaagt ttgatacaat
     1081 actaaaggtt gctacatacc aaagccaggc aaaggctatc catttttgtg ttttaaattg
     1141 aagtattgcc catacagtaa tacctgcatg aataaacaaa ctaagccaaa cccacagatt
     1201 gccaaaatct gtaataggta tttgattaaa actataatca tatgcaagat ttgaaggcca
     1261 aacaattttt tgcaggtata aaagccaggt taacaaaacg gttgccagac gttgcaaccg
     1321 atctgcatgt ataaatggat tattaaacac atcggtgctt atggcaccgg tattgccacc
     1381 tacagccatc tgacgtataa gtaaaaacag caacaccggt ataagcatat agcaactgtt
     1441 tattaatgct tttttaatgt gcattgtaaa tgcaaataca aagggataaa tagccagaaa
     1501 agttatggct gtttctttgc ttaaaagtgc caaaaaataa agtacagcag cttgtaataa
     1561 aacgaaactg ctcagggtgt ttttattttt taataagatt aatatgctgc ttaaaaaaaa
     1621 cagcaagcaa aaaatatcgt cacggccttt tatgtttata atcacttcgc tatggatggg
     1681 taatgcggca aaaagtaaag taacacttag tgtaaatgct ttaaatggcc aggcatattg
     1741 taagcaacga tacagtaaac aacaactgca tgcatataaa agcacattaa tggcatgtga
     1801 tatagcaggt gccaaacccc atagctgata ctccattgca aacgacacaa cacttaacgg
     1861 cctgtagcgc ccacctgtca acaccatggt gttggcatcg cttaaaccgg caaaagtatc
     1921 gttgccaaat attgaagcaa tgccggcaaa accttgttgc acaaatttgt tttttgccaa
     1981 tacaataaaa tcatctaagg catagccgtt aaacacggct gcaccataac ttaccaacac
     2041 caaaaggcaa attgccatgt atgcttttat atgatgcggt acgctgtaac ttgtttgctg
     2101 tattggtttt gaaactgggg gtttgggttt tttcatgcct caaataaaaa atcaaaatca
     2161 atgatgtgca atttagtatt accagctttg taaaagtttt ttagcctggt gttggtgtgt
     2221 acttttaagt ttgataactt tttgcaaggt gctttttgct tcattggttt ggcccaacgc
     2281 ttttagcgaa agagctttat agtatagagc atcttcgtac aacaatgctt ttttatcaat
     2341 acccgcttgc tcataatagc gtattgccat tgtataatta cctaaatgat aataacacat
     2401 gccggcataa aaattactgt tgctgttggt gctattatgc tgcaaaatgg tttcgaactt
     2461 tgctaatgat tccttgtatt gtgcagtgtt ataaagcgaa acagcactat cgtaaatgtc
     2521 gtatttaaat gatgcttcca aacccgattg tgcaacagca gcttgaggcg tttttttatt
     2581 tttacttacc gtctcaaccg atgctatgcg gttttttaat gtttcgtttt ctct
//
LOCUS       DAOMAA010000200         2627 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_33683_length_2627_cov_3.005443, whole genome shotgun sequence.
ACCESSION   DAOMAA010000200 DAOMAA010000000
VERSION     DAOMAA010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33683_length_2627_cov_3.005443"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="PLO59_03600"
     CDS             complement(<1..268)
                     /locus_tag="PLO59_03600"
                     /inference="COORDINATES: protein motif:HMM:NF014114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HQV00217.1"
                     /translation="MIQPDYLQPGDKIMVIAPARKVIQTELEPGVNLLRKHGFEVILA
                     QNVYAENNQFAGTDNLRAADLQNAIDRHDIKAIICARGGYGVSRI"
     gene            478..2520
                     /gene="metG"
                     /locus_tag="PLO59_03605"
     CDS             478..2520
                     /gene="metG"
                     /locus_tag="PLO59_03605"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF001100.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HQV00218.1"
                     /translation="MSRYLVTAALPYANGPIHIGHLAGCYLPADIFVRYQKLCGKDIL
                     FICGSDEHGVPITIKARNEGITPQQVVDKYHTLMKNAFADFGIAFDHYSRTSAPLHHQ
                     TAADFFKTLYDKNVFIEEVTQQYYDDEVNQFLADRYIVGTCPVCANTNAYGDQCENCG
                     TSLNPTDLINPKSKLSGKAPVLRETKNWFLPLDQYQEALTKWINSHENDWKPTVYGQC
                     FSWLNQGLQKRAMTRDLNWGVKVPIPNTDGKVLYVWFDAPIGYISATKELMNLRNQPD
                     AWKDYWQNADTKLLHFIGKDNIVFHCLIFPAMLMAHQQFILPQNVPANEFLNLEGQKI
                     STSRNWAVWLHEYLSDFKNKQDELRYVLCAIAPETKDADFSWKDFQARVNNELVAILG
                     NFVNRTLVLTHKFFDGKVPATEPLQPIDEIALQSIAYAYAEVSKKINEFKFRDALTEV
                     MNLARAGNKYLADTEPWKLFKTDTGTVATILHTSLEITAHLAVICQPFLPNTAAKIFG
                     MLNINPLQLSTYNANVIKVGHQLNQPGLLFEKIEDDAIQAQIDRLHAAPIADSNHSQS
                     AINALKPQTTFDDFTKMDLRIATITSAVAVPKTEKLLQLTLDTGNDTRTVVSGIALHH
                     KPENIIGKQVVLLANLAPRKIKGIESHGMILMAEDADGSLKFVSTDVFTTNGSTVA"
BASE COUNT          853 a          515 c          491 g          768 t
ORIGIN      
        1 taatacgcga aacaccatag ccgccacgtg cacaaataat ggctttaata tcatgccggt
       61 caattgcatt ttgcaaatcg gcagcacgca agttatcagt accggcaaac tgattatttt
      121 cggcatacac gttttgcgca agtattactt caaacccatg tttacgcaaa agatttacac
      181 cgggttcaag ttcagtttgt attaccttac gtgccggtgc aataaccata attttatctc
      241 cgggttgtaa atagtcgggt tgtatcattt gaaaaaaaaa ttaaaagcat tgcaaagtat
      301 tacttacagc gcttcataaa acaagcctgg tgtataattt gcgcaactgc cctaacactt
      361 taaacaatac ctaaagttca aataattttg gttgtgtggt atttctatat ttgccgccca
      421 attaaaaaat gcgttttaat gcattgaaaa cgtttaaaat tacagtaaac atcaactatg
      481 tcacgatacc ttgtaacagc tgcactacct tatgccaacg gccccataca tattggccat
      541 ttagccggat gttatttacc tgccgacatt tttgtgcgtt atcaaaaact ttgtggcaag
      601 gacatattat ttatttgcgg tagcgatgag catggcgtac ctataacaat taaagcacgt
      661 aatgaaggta taaccccaca acaagtggta gataaatatc acaccttaat gaaaaatgcc
      721 tttgccgatt ttggtattgc ctttgatcat tattcgcgta catcggcacc attgcatcac
      781 caaactgctg ccgatttttt taaaacccta tacgataaaa atgtgtttat tgaagaagtt
      841 acacagcaat attatgacga tgaagtaaat cagtttttag ccgacagata cattgttggc
      901 acatgcccgg tttgtgccaa taccaatgcc tatggcgacc aatgcgaaaa ctgcggcact
      961 tcgctaaacc ctaccgattt aattaatccg aaaagcaagt taagtggtaa agcgcctgtt
     1021 ttacgcgaaa ctaaaaattg gtttttacca cttgaccagt atcaagaagc gcttactaag
     1081 tggataaaca gccatgaaaa tgactggaaa cccaccgttt acggccaatg ttttagctgg
     1141 cttaatcaag gcttgcaaaa acgtgctatg acgcgcgatt taaactgggg tgtaaaagta
     1201 ccaataccca atacagatgg taaggtgctt tatgtttggt ttgatgcacc cattggttat
     1261 atatcggcca ctaaagagct catgaatttg cgcaatcaac ccgatgcctg gaaagattat
     1321 tggcaaaatg ctgatacaaa attgctgcat tttattggta aggataatat cgtatttcac
     1381 tgtctgattt ttccggccat gcttatggca catcaacaat ttatcttgcc gcaaaatgta
     1441 cctgctaatg agtttttaaa tttggaagga caaaaaattt ctacatcgcg caattgggct
     1501 gtttggttgc atgaatattt atcagacttt aaaaacaagc aagacgaatt acgttatgtg
     1561 ctatgtgcca ttgcacccga aactaaagat gccgatttta gttggaaaga ttttcaggca
     1621 agagttaata atgaattggt agccatattg ggtaattttg taaaccgtac tttagtgctc
     1681 acccacaaat tttttgacgg caaagtgccg gcaacagaac ctttgcaacc tatagatgaa
     1741 atagcacttc agtcaattgc ctatgcttat gccgaggtat caaaaaaaat aaatgagttt
     1801 aagtttaggg atgcattaac cgaagttatg aatttggcac gtgccggtaa taaatattta
     1861 gccgataccg aaccttggaa actatttaaa acagataccg gtaccgttgc caccatatta
     1921 catacttcat tagaaataac agcccattta gcggtaatat gccagccttt tttaccaaat
     1981 acagctgcta aaatttttgg tatgctcaat attaacccgc tgcagttatc tacgtataac
     2041 gctaatgtta ttaaagttgg tcatcaatta aaccagccgg ggttgctatt cgaaaaaata
     2101 gaagacgatg ccattcaagc acaaattgac agattacacg ctgcacccat agcggattct
     2161 aatcatagtc aatcagctat taatgcttta aaaccacaaa ctacgtttga tgattttact
     2221 aaaatggatt tacgcattgc aactattaca agtgctgttg ctgtacctaa aaccgaaaaa
     2281 ttattgcaac taaccttaga caccggtaat gatacacgta ctgttgtaag cggtattgca
     2341 ctacatcata aacccgaaaa tattattggt aaacaagtag tattactggc aaacttagcc
     2401 ccgcgaaaaa taaaaggtat agaatcgcat ggaatgatac taatggccga ggatgccgat
     2461 ggcagcctta agtttgtaag taccgatgta tttacaacaa acggcagtac ggtggcctaa
     2521 ttttaagata tgagtaagga agcacttttt atttcaggaa gtgccgtaag tggtagtccc
     2581 gacaggaatc gaacctgtat caaaagttta ggaaacttct attctat
//
LOCUS       DAOMAA010000201         2620 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_33890_length_2620_cov_2.524366, whole genome shotgun sequence.
ACCESSION   DAOMAA010000201 DAOMAA010000000
VERSION     DAOMAA010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33890_length_2620_cov_2.524366"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2135
                     /locus_tag="PLO59_03610"
     CDS             <1..2135
                     /locus_tag="PLO59_03610"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV00219.1"
                     /translation="EGKVTFYIQSMNGQNVFMQLFDGNGTTFLGGSYTSSNATFNVDG
                     LAAGTYYFRIKTYYNSEYVPYRFSVKLTTPTYLIDTEPNGTKATAKNITKNDSITGHI
                     GYYYNNLRDTFDYRKLTITEYGSLTFKVYSGNGQNVYAQLFDADGTTYLAGSYTSSTA
                     QWTRNDMAPGTYYLRIHTYYVNSEFVPYSLKLTFTPVVATDLEPNATMATATTLNVNT
                     PTNGCFGYYNNGVRDTNDWFKVTMPEDGKAIFSLGYAVAVTGNYFLEVYDAQGVIRHS
                     QYASGATLTFTKADIRKGTYYVRVHGYYNHEYSQYLLNVTFTSTNPTDAETNNTASTA
                     KHIAGYAVRNGNIGYNAFGTATDTVDWHRMGHWGVGPMNLIIKKVANPDGVMPSINFK
                     LYGDTTAAPLYNFDLTADSTNVSYTLAKKVYYIKLTKLGVTYGGYNITANYRDTCSLV
                     IAVTTHIPDSGCNRGTLVYDITRGVGPYVVQLYKDGVASGAPINTSGTATYNNVDGGV
                     YYITARSSQAVDCDKASSIRAIAGTPLGIQKLQITSTTAVLRWTAQPCVDGFIMAYRI
                     PPSLVFTFDTVPAGKYRDTIFNLTPGTRYRCKIASFVTYDGTTYVGNYSDLFSIITPL
                     RQGDEMIAENENLLVYPNPANDVIQVALPTDNGTLQIANMMGQVVKSVAVSTTFNTQV
                     SVYDLPKGVYLMQYISNNKSYTTRFIKE"
     gene            complement(2194..>2620)
                     /locus_tag="PLO59_03615"
     CDS             complement(2194..>2620)
                     /locus_tag="PLO59_03615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQV00220.1"
                     /translation="QKPDAKLLIAGASPTLAIKQLQSNSITVSGWVDDIRTSFAHSKI
                     MIAPMLISIGLQNKILQAMAMKVPCIVSSLANNAVGAKHNQEVLIAATAAEYASCIIS
                     LLSDETQSQTIAENAYTFVKQNFNWQVQTQRLEQYMIAS"
BASE COUNT          787 a          547 c          492 g          794 t
ORIGIN      
        1 ttgaaggcaa ggtaacattt tacattcagt caatgaatgg ccaaaatgtt tttatgcagt
       61 tatttgatgg caatggcact acctttttgg gtggcagcta tacttcaagt aatgctacat
      121 ttaatgtaga tggattggct gccggtactt attacttccg tataaaaacc tattataact
      181 ctgaatatgt gccttaccgt ttcagcgtta aattaactac accaacttat ttaatagata
      241 ccgaaccaaa tggtacaaaa gctacagcta aaaacattac taaaaacgat agtattaccg
      301 gccatatagg ttattattac aacaacctgc gcgatacatt tgattatcgt aaacttacta
      361 ttaccgaata tggttcacta acatttaaag tatatagcgg taatggtcaa aatgtgtatg
      421 cccaattgtt tgatgccgat ggcactactt atttggcagg cagttacaca tcttcaaccg
      481 cccaatggac ccgaaacgat atggcaccgg gtacctatta cctgcgcatt catacttatt
      541 atgtaaattc cgaatttgta ccttattctt taaagctaac ttttactccg gttgtagcaa
      601 ccgatttaga gcccaatgca accatggcca cggcaacaac tttaaatgta aacacgccca
      661 ccaatggttg ttttggatac tataataatg gcgtacgcga taccaacgac tggtttaaag
      721 taaccatgcc cgaagacggt aaagccattt ttagtttagg atatgcagta gcagttacag
      781 gtaattattt tttagaagtg tatgatgctc agggtgtaat ccggcattca caatatgcat
      841 cgggagctac tttaaccttt accaaagccg acatcagaaa aggtacttat tatgtgcgtg
      901 tacatggtta ttacaatcac gaatactcgc aatatctatt aaacgtaacc tttaccagta
      961 ccaacccaac cgatgccgaa accaacaata cagcatcaac tgccaaacac attgccggtt
     1021 atgcagtacg taatggcaat attggttaca atgcttttgg taccgctaca gatacagtag
     1081 attggcaccg tatgggacat tggggtgttg ggcctatgaa tttaattatt aaaaaagtgg
     1141 ccaaccccga tggtgttatg ccaagcatta actttaaatt gtatggcgat actacggctg
     1201 caccattata caatttcgac ttaactgccg actcaaccaa cgtaagttac accttagcca
     1261 aaaaagtata ttacataaaa cttactaaat taggtgttac ttatggcggc tataatatta
     1321 cagccaacta ccgcgatacc tgcagtttgg taattgccgt taccacacac atacccgact
     1381 caggttgtaa ccgtggtaca ttggtatatg atattacacg cggtgtaggc ccttatgtag
     1441 tacaacttta taaagacggt gttgcttcgg gcgcgccaat taatacaagc ggtacagcaa
     1501 cttataacaa tgtagatggt ggtgtttatt acattactgc acgcagcagc caagccgttg
     1561 attgcgataa agcttcgagt attcgtgcta ttgccggtac acctttaggc attcagaaac
     1621 ttcaaatcac aagcactact gctgttttac gttggactgc ccaaccatgt gttgatggtt
     1681 ttataatggc ttatcgcata ccaccatcat tggtgtttac gtttgacact gtacctgcag
     1741 gcaaataccg cgacaccatt tttaacttaa caccgggtac acgctaccgt tgcaaaattg
     1801 cttcatttgt tacgtatgat ggcactacgt atgtgggtaa ctactccgat ttgtttagca
     1861 ttattacacc attgcgccaa ggcgatgaga tgattgccga aaacgaaaac ctgttggtat
     1921 atcccaatcc ggctaacgat gtaattcagg tagccttgcc aaccgataat ggtactttac
     1981 aaattgccaa tatgatgggt caagtagtta aatcggttgc agtttcaact acgtttaaca
     2041 cgcaggttag cgtttacgat ttacccaaag gcgtttacct gatgcagtac atttcgaata
     2101 ataaatcata cacaacccgg tttattaaag agtaagtttt attaaaccga tattaaaaaa
     2161 agcagcgcta caataattgt ggcgctgctt tttttatgaa gctatcatgt actgctccaa
     2221 cctttgtgtt tgaacctgcc aattaaaatt ttgctttaca aaagtatatg cattttcagc
     2281 tatggtttgt gattgtgttt catcgcttaa taaagaaata atacaggatg catactcggc
     2341 cgcagtagct gctattaata cttcttggtt atgctttgct cctacagcat tatttgcaag
     2401 actgcttaca atgcaaggca ctttcatggc catagcctgt agtattttgt tttgtaaccc
     2461 aatacttata agcatgggcg caatcattat tttcgaatgc gcaaacgatg ttcgaatatc
     2521 atccacccaa cccgatacgg taatactgtt actttgcaac tgcttaatag ccaaagttgg
     2581 cgatgcacct gcaataagta attttgcatc cggtttttgc
//
LOCUS       DAOMAA010000202         2618 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_33932_length_2618_cov_2.570425, whole genome shotgun sequence.
ACCESSION   DAOMAA010000202 DAOMAA010000000
VERSION     DAOMAA010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33932_length_2618_cov_2.570425"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..1049
                     /locus_tag="PLO59_03620"
     CDS             153..1049
                     /locus_tag="PLO59_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014019429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HQV00221.1"
                     /translation="MKYIDPHIHMCSRTTDDYEAMKQAGIVAVIEPAFWQGQLRTAVG
                     TFKDYYQSLIGWERFRASQFGIKHFCTIGLNSKEANNESLAEQVMEILPMFIYKEGVV
                     GIGEIGYDDQTAMEDKFFRQQLVLAKDAQLPVQIHTPHRDKKTGTTRSMDVCMEHGLA
                     PDWVIVDHNNEETVKETLDRGFWAAFTIYPHTKMGSQRMVEIVKQYGSDGIFINSAAD
                     WGVSDPLAVPKTAALMLTHGISEHDVQKVCYTNALIAFGKSGQFNEADWLQAAPADQQ
                     LKYHGNSILRGGQKPANTGEIV"
     gene            1052..1942
                     /gene="eboC"
                     /locus_tag="PLO59_03625"
     CDS             1052..1942
                     /gene="eboC"
                     /locus_tag="PLO59_03625"
                     /inference="COORDINATES: protein motif:HMM:NF035940.1"
                     /note="EboC, a homolog the polyprenyltransferase UbiA,
                     belongs to system of proteins involved in the trafficking
                     of precursor metabolites to an extracytoplasmic
                     compartment so that the biosynthesis of certain natural
                     products, such as scytonemin, can be completed; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like protein EboC"
                     /protein_id="HQV00222.1"
                     /translation="MPINFRAYLTLTRPPNIITSMADVIAATCIACTVAQMHLTDNII
                     EILCLAFASCFLYAGGVVMNDVADISIDKIERPERPIPAKLVSKTGATIWALLLSATA
                     IVLASWVSIVSAILAILIVIASYVYDFYGKHYAIGPINMGLCRALNFSMGLSISLQVL
                     QSYWWLSLIAVVYIAAITMISKGEVSGGNQSAIRNAAIMFGFVIGVLAFIAITQQTHF
                     YAALFFVALFTFYLYKPLIMAYQQPQPALIRKAVKAGVIGIILLDACLLAAFMGLLPA
                     LFLCALLPISLIIAKIFAVT"
     gene            1989..>2618
                     /gene="aroB"
                     /locus_tag="PLO59_03630"
     CDS             1989..>2618
                     /gene="aroB"
                     /locus_tag="PLO59_03630"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:NF013887.2"
                     /note="catalyzes the formation of 3-dehydroquinate from
                     3-deoxy-arabino-heptulonate 7-phosphate; functions in
                     aromatic amino acid biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HQV00223.1"
                     /translation="MELIKQNFAVNYTYPVLFINSLFEPGKNKFLKIFEELAIADGAR
                     FLIVVDSNVAKAQPALIPSIELFFRLHPFNLLASPVLIDGGEVCKNSLSYTESIWQLI
                     NKYGVDRHAYLMAIGGGALLDVAGFAASVAHRGIRHIRVPTTVLSQNDSGVGVKNGIN
                     YFGKKNFLGCFATPFAVINDSAFLNTLSNRDFRSGIAEAIKVALIKDADF"
BASE COUNT          778 a          465 c          559 g          816 t
ORIGIN      
        1 aaaggctgca tcgcttcttt tttacagtaa tgaatcggca aaggctttgg gtttattacc
       61 acaaaagcaa catcaaacac ttatcgaaaa caacgagctg aactggcaaa cattgggtgc
      121 cttggtttgg cataacaatt aatttatcaa acatgaaata tattgatccg cacatacaca
      181 tgtgctcgcg tacaacggat gattacgaag ccatgaaaca agccggcatt gttgccgtta
      241 ttgagcctgc tttttggcaa gggcaattac gaactgcagt tggtacattt aaagattatt
      301 atcaaagctt gattggttgg gaacgattca gagcttcgca atttggtatt aagcattttt
      361 gcaccattgg cctcaacagc aaagaagcca ataacgaaag cctggccgaa caggttatgg
      421 aaatattgcc catgtttatt tataaagaag gcgtagtggg cattggcgaa attggttatg
      481 atgatcagac agccatggaa gataagtttt tcaggcaaca attagttttg gcaaaagacg
      541 cacaacttcc ggtacaaata catacaccgc accgcgataa aaaaacgggt actacccgca
      601 gtatggatgt ttgtatggaa catggcctgg cacctgattg ggtaattgtt gatcacaaca
      661 atgaggaaac cgttaaagaa actttagatc gtggcttttg ggctgccttt accatctacc
      721 cacatacaaa aatgggtagc caacgtatgg ttgaaattgt gaaacaatac ggaagcgatg
      781 gtatttttat aaactctgct gccgactggg gcgttagcga tcctttagct gtaccaaaaa
      841 cagcggcttt aatgttaaca cacggtataa gtgaacacga tgtgcaaaaa gtttgttaca
      901 ccaatgcatt aattgctttc gggaaaagcg gacagtttaa cgaggccgat tggttgcagg
      961 cagcacctgc agatcagcag ttaaaatatc acggtaattc tattttgcgt ggcggacaaa
     1021 aaccggcaaa tacaggtgag atagtataaa catgcccatt aactttagag cttacttaac
     1081 cttaacaagg ccgcccaaca taattacgtc tatggccgat gttattgcag caacatgcat
     1141 tgcatgtacc gttgcccaaa tgcatttaac cgataacatt atagagatac tttgtttagc
     1201 atttgccagt tgttttttat atgcaggcgg tgttgtaatg aacgatgttg ccgatattag
     1261 cattgataaa attgaaagac ctgaacgacc tataccggcc aaattggtaa gtaaaacagg
     1321 tgcaacaata tgggcacttt tgctctcggc tactgctatt gtattggcaa gttgggttag
     1381 tattgtctca gcaattttgg ctatcctaat tgtaatagca tcttatgttt atgattttta
     1441 cggcaagcat tatgcaatag gacccattaa tatgggcttg tgccgtgcat taaactttag
     1501 tatgggtttg tcaattagtt tgcaggtgtt acaaagttac tggtggctta gcctaatagc
     1561 cgtagtttat attgctgcaa ttaccatgat tagcaaaggt gaggtaagtg gaggtaacca
     1621 aagtgctata cgtaatgcgg caattatgtt tgggtttgta attggtgtac tggcttttat
     1681 agctattacc caacaaacac atttttatgc tgcattgttt tttgtggcat tatttacctt
     1741 ttatttatat aagccgctga taatggcata ccaacaaccc caacccgcac ttatccgtaa
     1801 ggctgtaaaa gcaggtgtta ttggcattat acttttagat gcttgtttat tggctgcatt
     1861 tatgggtttg ctgcctgcat tatttttatg tgctttgttg ccaattagtt taattattgc
     1921 aaagatattt gctgttacat aaatcgcatg gctttttata atttgcacaa caattaaaaa
     1981 ttattttgat ggagcttata aagcaaaact ttgctgtaaa ttatacctac cctgtacttt
     2041 ttatcaattc attatttgaa ccgggcaaaa acaaattttt aaaaatattt gaggagttag
     2101 ctattgccga tggtgcccgt tttttaattg ttgttgatag taatgttgca aaagcacaac
     2161 cggcacttat acccagtatt gaattgtttt tcaggttgca ccctttcaat ttgctggcat
     2221 cgcctgtttt aattgatggc ggtgaagttt gtaaaaacag tttaagctat acagaaagta
     2281 tatggcaact aataaataaa tatggtgttg acaggcatgc atatcttatg gctattggtg
     2341 gtggtgcatt attagatgtt gctgggtttg cagcttcagt tgcacatcgg ggtatcaggc
     2401 atatccgggt tccaacaact gttttatcac aaaacgattc gggcgtgggc gtaaaaaatg
     2461 gcataaatta ttttggcaag aaaaactttt taggatgctt cgcaacaccg tttgccgtta
     2521 taaacgacag tgctttttta aacacactca gcaatcggga tttcaggtcg ggcatagccg
     2581 aagcaataaa agttgctttg ataaaagatg ccgatttt
//
LOCUS       DAOMAA010000203         2616 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_33978_length_2616_cov_2.957048, whole genome shotgun sequence.
ACCESSION   DAOMAA010000203 DAOMAA010000000
VERSION     DAOMAA010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33978_length_2616_cov_2.957048"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="PLO59_03635"
     CDS             complement(<1..216)
                     /locus_tag="PLO59_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00224.1"
                     /translation="MVNAASVLTLVKDGNAVYSIHAVTENQKQAAVILNKYVTAISGT
                     TLPVVSIAKDLQIVFTPPPDVKFNVDQM"
     gene            complement(266..1285)
                     /gene="murB"
                     /locus_tag="PLO59_03640"
     CDS             complement(266..1285)
                     /gene="murB"
                     /locus_tag="PLO59_03640"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012929628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HQV00225.1"
                     /translation="MSEILNNISLKPLNTFGIDVQATSFVKIDSYNHLMQLVQMPVFK
                     EQPHYILGGGSNLLLTQNYNGLMVQIANKGIEVLKETTQHVYVKVQAGEVWHQFVIWC
                     IAQNYAGVENLSLIPGSVGAGPMQNIGAYGVELKDVFFELEALHIPTNTLKTFDAGQC
                     NFGYRQSIFKTELKSQCIILSVTFRLNKQPEFKVSYGAIKEQLELMQIKTLSIKAISD
                     AVISIRKSKLPNPEEIGNAGSFFKNPTISKEQYKMLISNYPDCVGYALPNDEIKLAAG
                     WLIEQCGFKGKRIGNTGAHAKQALVLVNYGGATGAEVFACAQQIQQEVKLKFDVCLDM
                     EVNIL"
     gene            complement(1370..1495)
                     /locus_tag="PLO59_03645"
     CDS             complement(1370..1495)
                     /locus_tag="PLO59_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00226.1"
                     /translation="MKKLMALVLVAFVVTSFTACKQHEKCPAYGKANYKSVKKAS"
     gene            complement(1604..2317)
                     /gene="plsY"
                     /locus_tag="PLO59_03650"
     CDS             complement(1604..2317)
                     /gene="plsY"
                     /locus_tag="PLO59_03650"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HQV00227.1"
                     /translation="MINYTTVFACVLAYLLGSIPSAVWVGRTFYGIDVREYGSGNAGA
                     TNTFRVLGKKAGIPVLLMDIAKGYLAVQMATILSLYLGDLKTVSMLGTSIDFASIKTP
                     LPGTQQFVNFKLALGVCALIGHIFPVYVGFRGGKGVATTLGIVAGCHPQAALICAAIF
                     FITFIITNYISLSSMVAGFAFPIIIWLVFGETITTMNVFAMTVAIIILITHQKNIERL
                     LKGEESKMYLYQRKATKQV"
BASE COUNT          831 a          534 c          409 g          842 t
ORIGIN      
        1 catttggtca acattaaatt ttacatcagg aggtggtgta aaaacaattt gcaaatcttt
       61 agcaatgcta acaacgggta gtgttgtgcc acttattgca gttacgtact tattaagaat
      121 aactgctgcc tgcttttgat tttcagttac tgcatgtatg ctataaactg catttccatc
      181 ttttaccagc gttaaaactg atgctgcatt taccatactg catgtgcaaa ttgacaataa
      241 aacgaatata aaatgtatca ttattttata aaatatttac ttccatatct aaacaaacat
      301 caaactttag tttaacctct tgttgaattt gttgcgcaca tgcaaacact tcggcaccgg
      361 ttgcaccgcc ataatttaca agtaccaaag cctgctttgc atgtgcgcct gtgttgccaa
      421 tacgcttgcc cttaaaacca cattgctcga taagccaacc tgctgccagt ttaatttcat
      481 cgtttggcaa tgcatatcca acacaatcgg gatagtttga aatcagcatt ttgtattgct
      541 ccttacttat ggtagggttt ttaaagaaac tacctgcatt accaatttct tcgggattgg
      601 gcagcttgct ttttctgata ctgataacgg catcgctgat agccttaata cttaaagttt
      661 ttatttgcat caactccaac tgctctttaa ttgccccata agaaacttta aattcgggtt
      721 gcttgtttag cctgaaagta actgataaaa tgatgcattg gctttttaat tcggttttaa
      781 atatactttg gcgataacca aaattacatt ggccggcatc gaacgttttt aatgtattag
      841 taggtatgtg cagcgcttcg agttcaaaaa aaacatcctt tagttcaaca ccatatgcac
      901 caatattttg cataggcccc gcacccacac tgccgggtat taacgataga ttttcgacac
      961 cggcataatt ttgtgcaatg caccaaataa caaattgatg ccacacctcg cctgcctgta
     1021 cttttacata tacgtgctgt gtggtttctt tcaacacttc aataccttta ttagcaattt
     1081 gcaccatcag cccattataa ttttgggtta gcagtaaatt actgccacca cctaaaatgt
     1141 aatgtggctg ctctttaaaa accggcattt gcaccaattg cattaagtga ttgtaactat
     1201 ctatttttac gaaagatgta gcttgtacat caataccaaa agtatttaag ggtttaaggg
     1261 atatattgtt taatatttca ctcataagtg cttacgtata aaatcaacaa aaaacgctgc
     1321 acattaattt atgagcagcg ttactattat taaaaagccg ataactttat tatgatgcct
     1381 tttttacaga tttatagttt gctttaccgt atgcaggaca tttttcatgt tgcttgcatg
     1441 cagtaaacga tgtaactacg aaggctacta aaaccaaagc cattaacttt ttcatttttt
     1501 tttaattaat tatttgcatg caaagctact atttacggct taaatcacgc atatactttt
     1561 caacaaaatt gtgttattat ttaccgttta tgcagcaacc tgattaaacc tgcttggtag
     1621 ctttacgctg atacaaatac attttcgact cttcgccttt aagcaatcgt tcgatgtttt
     1681 tctgatgtgt aattaaaata atgatggcta ccgtcatggc aaacacattc atagttgtaa
     1741 tggtttcgcc aaataccagc caaattataa taggaaaagc aaaccctgcc accatgctgc
     1801 ttaacgatat ataattagtt attataaagg ttataaaaaa aatagccgca caaataagtg
     1861 ccgcttgcgg atgacaacct gccacaatac ctaaagtggt ggctacacct ttgccgcctc
     1921 taaagccaac atacaccggg aaaatatggc ctattaaagc acaaactccc aatgccaatt
     1981 taaaattaac gaactgttgt gtgcccggta atggtgtttt tatgctggca aaatcaatag
     2041 aggtgcccaa catcgaaaca gtttttaaat cgcccaaata caaacttaaa atagtagcca
     2101 tttgcacggc taaataacct ttggcaatat ccataagtaa aacagggatg cctgcttttt
     2161 tgcccaatac cctgaaagta tttgtagcac ctgcattacc actaccatac tcgcgcacat
     2221 caatgccgta aaaggtacga cctacccaaa ctgccgatgg aattgaaccc agcaaatatg
     2281 ccaacacgca ggcaaataca gtagtgtaat taatcatata gggttgcgaa tgtaatttat
     2341 tttaactttt taaaccactc aaatgcatgg ccataaattg ctgcctcttt catgattatt
     2401 tggtgaaatt tatgcttaac acaaatttta tttctgcatg aaacccaata aaatcaatgc
     2461 acttagcaac caatggtgta tgaagcaaaa tttatttgta acttttttta atagccatac
     2521 atctcaccta caactaattc aaaaactaaa aaactaaatc gaatgaatat cattaaacta
     2581 attaccctta ccgcattatt tgttgtatgc ggttta
//
LOCUS       DAOMAA010000204         2608 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_34196_length_2608_cov_2.477086, whole genome shotgun sequence.
ACCESSION   DAOMAA010000204 DAOMAA010000000
VERSION     DAOMAA010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34196_length_2608_cov_2.477086"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(201..1037)
                     /locus_tag="PLO59_03655"
     CDS             complement(201..1037)
                     /locus_tag="PLO59_03655"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV00228.1"
                     /translation="MKKNLLILFTALFSSTNAFAQLPNNSFETWTAGLPNGWYSSLIP
                     GYVLLEQSSDAHTGNYSAKLNVANLGGNPIPTSLLTGNGNSFAVPISYIPASVGFWYK
                     ASLNTGDQLVAAAYVYGGGGSIIGVGSVSLNSAGAYTQALLPITNIGGITTADSIALT
                     FLITNPLGSTTIGSNANIDDVELLITPGINDVKSSYSLDINPNIVTDKLNITYTALTA
                     GTSSIAIYNTAGILILEKNMQVNSGVNQLTLSLNDLASGTFVCTVKTKNLVTAKYFVL
                     SR"
     gene            1512..>2608
                     /locus_tag="PLO59_03660"
     CDS             1512..>2608
                     /locus_tag="PLO59_03660"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQV00229.1"
                     /translation="MNAKPNHLNDSIKSISKNISDSTYITLSDKITSDFLNNNQPEQA
                     LRFIEDQLKQFNHTSKRCHQLLKLQAKVNYRIKNYTMANALADSLLKLKPDTFQIIDA
                     YRIKGQVCFRVDDYLNAIENFLKARNLILKSSNKNGLDDVLGNLASCYQTIGLLPTAL
                     TFAQEALAISRQANDTASMCNAYLNLGNINKDIVEWDEAEINYLAGLALAKQFTDKQV
                     LVYLYSALGTIERTKGNTTTALQYQLNALSEAQFISNNHLIAGVLNNIANTLLDAGKT
                     AEAKAYFVKANSLAKETADNRNLALTNISLGFLATEEKHFELSNNYLLDALTYSKQIN
                     NLEYISQAMIGMYDNYKAMNKPAQALYYYEM"
BASE COUNT          913 a          417 c          464 g          814 t
ORIGIN      
        1 atgcggttat ggaatatttt attgattctt atgagacgtt aaaaggcaga aatattgaag
       61 aggaagacag agaatttctt cagagaaagt ttgacaaaat gtatgtaacg aaagatattt
      121 atgctattta caataaactg cttgaagtgt atattgtaag cttaagcatt acataaatac
      181 ataatgccaa aatcaattgt ttatctactt agcacaaaat attttgcggt taccaaattt
      241 tttgttttta cagtacatac aaaggtgcct gaagccaaat cattaaggct aagggtaagc
      301 tggttaacac ctgaatttac ctgcatgttt ttttcaagaa ttaaaatacc ggctgtattg
      361 tagatagcaa ttgaacttgt gcctgccgtt aaagctgtgt atgtaatgtt taatttatca
      421 gttacaatat tgggatttat atcgagacta tagctacttt ttacatcgtt tataccaggg
      481 gttatcaaaa gttcaacatc gtcaatgttg gcatttgagc caatggttgt tgatccaagc
      541 ggattggtaa ttaaaaaagt taaagctatc gaatcggcag tggtaatgcc tccaatatta
      601 gttatgggta acaatgcctg cgtatatgca ccggctgagt tgagcgaaac actaccaaca
      661 cctataatgg atccgcctcc accataaaca taagcagcag caacaagctg atcgcctgtg
      721 ttcagacttg ctttatacca aaaaccaaca cttgccggta tatatgaaat gggtactgca
      781 aagctattgc cgttacccgt tagtaacgat gtaggtatgg ggttgccacc taagttggct
      841 acatttaatt tggcactata attacctgta tgcgcatcgc tgctttgctc aagcaaaaca
      901 tagccgggta taagcgatga gtaccagccg ttaggtaaac cggctgtcca ggtttcgaaa
      961 ctattattgg gtagctgggc aaatgcattt gtcgaactaa acaaagcagt aaataaaatg
     1021 agtaggtttt ttttcatgat taaggttttt aaagtttaaa aagttgttat cctaaaagta
     1081 gaaataaata tccattttaa aatgcaagta gtgcaaactt tacattgaat tttatttgta
     1141 tgtgatgtat ttaattaaaa atgggagtta tactaccaaa gtagtattat ttaaagttgc
     1201 atgtatgttg agttttgcat gattcaaact taaataacca ttacgtgtaa ttgtaaaata
     1261 taatcctttt acggcaaatt aaaaagatgt ttttgcttaa taaattgaag tatcacaatg
     1321 atttataatt ctgcattgca tttgcaaatt gaataattag taatattaaa atttgtagat
     1381 aataaaattg gaattacccg cactcatttt accgaaagca gttcgggttg aactttaaaa
     1441 gcaaagtcct ttgtatgatt aaaaataagt gcaacttcat ttttggtttg ttcttcatgc
     1501 ttcaattaaa tttgaatgca aagccaaatc atctcaatga ttctataaaa tcaattagca
     1561 aaaacataag cgactcaact tatattactt tatctgataa aataacatct gactttttaa
     1621 ataataatca accggaacaa gcactccgct ttatcgaaga tcagcttaag caatttaacc
     1681 atacatctaa acgatgccat caacttttaa agttgcaagc caaagttaat tatcgaatta
     1741 aaaattatac gatggcaaat gcactggccg atagcttgct aaaattaaag cctgatacat
     1801 ttcaaattat tgatgcatac aggattaaag gccaagtttg tttcagggtt gatgactatt
     1861 taaatgcaat cgaaaatttt ttaaaagcac gcaaccttat tttaaaaagc agcaataaaa
     1921 acggtttaga tgatgtgttg ggtaatctgg caagttgcta tcaaaccatt ggtttgttac
     1981 ccacagcctt aacatttgca caagaagcac ttgcaatttc gcggcaggct aacgatacag
     2041 caagcatgtg taatgcctat ttgaatttgg gaaatattaa taaggatatt gttgaatggg
     2101 atgaggcgga aataaattat ttggccggtt tagcattagc aaaacaattt actgataaac
     2161 aagtgctcgt atatttatat tcagcattgg gaaccatcga gcgtacaaaa ggtaacacca
     2221 ctacagcttt gcaatatcaa ttaaatgcat taagcgaggc acaatttatt agcaacaacc
     2281 atcttattgc aggcgtatta aacaatatag ccaacacatt attagatgcc ggcaaaacag
     2341 ccgaagccaa agcttatttt gttaaagcaa atagcttagc aaaagaaaca gccgacaaca
     2401 gaaacttggc tcttaccaat atcagtttgg gttttttggc tactgaagaa aagcattttg
     2461 agttatcaaa caattacctg ttagatgcat tgacctatag taaacaaatt aataatttgg
     2521 aatacataag tcaggcaatg attggtatgt acgacaatta taaagcaatg aacaagcctg
     2581 cacaagcttt gtactattac gaaatgta
//
LOCUS       DAOMAA010000205         2598 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_34432_length_2598_cov_3.096736, whole genome shotgun sequence.
ACCESSION   DAOMAA010000205 DAOMAA010000000
VERSION     DAOMAA010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34432_length_2598_cov_3.096736"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1631)
                     /locus_tag="PLO59_03665"
     CDS             complement(<1..1631)
                     /locus_tag="PLO59_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00230.1"
                     /translation="MFNSPVFDIALGLIFIFLLYSLLATIIAEMVVEWIGLRARMLRQ
                     AIGRMLNDDHLQTMNRKEKVSNRAANFLLYEKTDFNDSLAGRFYSHPSINYLSSGAYH
                     SKPSYLSNENFAATLIQMMREKGIGETDLEKIRFCISNNLLQMEPDTYRQFTHLLLNS
                     GQSVDAFKAQLMLWFDNTMDRANGWYKRKMQLILFWVGFALAVCFNVDCIRIAGILAE
                     DKDARNQLAQLSIQTADTNSTISKALYVSKDSAATYEQLRKSYQELDKARAEANQLLG
                     LGWSKCDSCETAVAGGAVVKRKAAAIDVAGCVLVKSFNPLQTTFWSFIITALALTLGA
                     PFWFDLLRKLVSIRSAGVKPEEKVTPAANTTSAVVVTAKPTEQPTAHSNATMVATTLT
                     TDQFIEIYSREIQSIAGVIIIRKYFYLEGSVKKSGVQVNVKTQADADKVTNQYGNLCA
                     KNNIRLHTIINTEAKIQAGQINTATNPNLGISNSELLHGWGSFGFMAKGIANESNYYL
                     VTCFHVLNGSYNWYNGKQNGQIQNFNRKRIGTSFDG"
     gene            1863..2399
                     /locus_tag="PLO59_03670"
     CDS             1863..2399
                     /locus_tag="PLO59_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00231.1"
                     /translation="MLLQIACETEKDKNGACDAGSESVEHVLLTNINDSIKPFSFPVV
                     NGKTIFYKYFNGKVTFFIAYNKVNICTIEHLNVTYIVSPSDSAQTLPMKIYGEAYWSA
                     YSDDLVLVSGPIQPSPQVYTGVLELGLQQAFGDGPGDVDFYVAVEFDTQGSFEADSLY
                     FKHHIDLMAIEFKSDNYL"
BASE COUNT          777 a          520 c          483 g          818 t
ORIGIN      
        1 ccatcaaatg atgtacctat acgtttgcgg ttaaagtttt gtatttgtcc gttttgtttg
       61 ccattgtacc aattgtagct accatttaaa acatgaaaac aggtaactaa ataataattg
      121 ctttcattag ctataccctt agccataaaa ccaaagctgc cccagccatg caacagttcg
      181 ctattagaaa tgcctaaatt tgggtttgta gccgtattaa tttgcccggc ttgtattttt
      241 gcttcggtat tgatgatggt atgtaaacga atattattct ttgcacacag gttgccatat
      301 tgattggtta ccttatcggc atcagcttgc gtttttacat ttacctgtac accacttttt
      361 ttaaccgagc cttctaaata aaagtacttg cgtattataa taacaccggc aatactttgt
      421 atttcgcggc tataaatttc aataaactga tcggtggtaa gggtggttgc caccatagta
      481 gcattgctgt gtgcagtggg ttgctcagtg ggcttggccg taactactac tgccgatgta
      541 gtgttggcag ccggtgttac tttttcttca ggtttaactc cggcacttcg tatgcttacc
      601 aacttacgta ataaatcaaa ccaaaatggt gcacctaacg ttagcgccaa agccgtaatt
      661 ataaacgacc aaaaagtagt ttgcaacgga ttaaaacttt taaccaacac acaccctgca
      721 acatctattg cagcagcttt ccttttaacc actgctccac cggcaacagc tgtttcacaa
      781 ctatcgcatt tcgaccagcc caaacccaat aactgatttg cttctgcacg cgctttatca
      841 agttcttggt acgacttcct taactgctca tacgttgcag cactgtcttt tgaaacatac
      901 aatgcttttg atatggtact gttggtatcg gcagtttgta ttgataattg cgccaattgg
      961 ttgcgtgcat ctttgtcttc ggccaaaatg ccggcaatac gaatacaatc aacattaaag
     1021 cataccgcca aggcaaaacc cacccaaaaa agtattaatt gcattttgcg tttataccat
     1081 ccgttagctc tgtccatggt attatcaaac caaagcatta attgtgcttt aaatgcatca
     1141 accgattgcc cgctgtttaa taataaatgc gtaaactgtc tgtaagtatc aggctccatt
     1201 tgcaacaggt tattggatat acagaaccgg attttctcaa ggtcggtttc accaattcct
     1261 ttttcgcgca tcatttgtat cagcgtagct gcaaagtttt cgttgcttaa ataggatggt
     1321 ttgctgtggt aagcacctga actcagataa ttaatagacg gatgactata aaaccggcct
     1381 gctaatgaat cgttgaaatc ggttttttca tacaataaaa aattagcagc tctgttgctc
     1441 actttttctt ttctattcat ggtttgcaga tggtcatcgt taagcatgcg acctattgcc
     1501 tgccgcaaca tacgcgcacg caggccaatc cattcaacaa ccatttcggc tatgatggtt
     1561 gcaagcaaac tatataggag gaaaataaaa attaagccga gcgcaatgtc gaaaacaggt
     1621 gagttaaaca tggcatggtt agtttattag gttggtggca ctaacttaat atatcaaata
     1681 gccgatgtca agtaaaatct acacaaatgt ttatgtatta aaaggcgttt aatatattta
     1741 catgtgcttt agttattagg ttagagaaaa tgatgtccat tacttttcaa taatattatg
     1801 aacgtactaa acctaaaatt atgaaaccaa atactaccct cttatttttt gtatgttgtt
     1861 tgatgctcct acaaattgcc tgcgaaaccg aaaaagacaa aaatggcgct tgcgatgcag
     1921 gttccgaatc ggtagaacat gtattgctga caaacatcaa cgatagtatt aaacccttta
     1981 gctttccggt agtaaatggc aaaactattt tttataaata ctttaatggt aaggttacct
     2041 tttttattgc ttacaataaa gtaaacatct gcactattga acatctgaat gtaacttaca
     2101 ttgtttcgcc atctgactcg gcacaaacct tgcccatgaa aatatatggc gaggcatact
     2161 ggagtgctta tagcgatgat ttggttttag tttcgggccc tatacaaccc agcccacaag
     2221 tttataccgg ggttttagaa cttgggctgc aacaggcatt tggcgatggc ccgggcgatg
     2281 ttgattttta tgtggcagtt gagtttgata cacaaggttc gtttgaggcc gacagtttgt
     2341 attttaaaca tcatattgat ttgatggcta ttgaattcaa gtcggataat tatttataac
     2401 atgcatgtac aaaaacaggc attactaaaa gtaggcatac aaacttgaat taaagcatga
     2461 caggaaaata tttggcagca cggttaactg aagttttttt aaatggccaa tggattgcgc
     2521 aaaccaatta taaagaacaa ctaaccaata ttacctggca acaagctatt tacaagcagc
     2581 gcaacctcaa ttcaattg
//
LOCUS       DAOMAA010000206         2588 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_34662_length_2588_cov_2.930122, whole genome shotgun sequence.
ACCESSION   DAOMAA010000206 DAOMAA010000000
VERSION     DAOMAA010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34662_length_2588_cov_2.930122"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..357
                     /locus_tag="PLO59_03675"
     CDS             91..357
                     /locus_tag="PLO59_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012439131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HQV00232.1"
                     /translation="MQCSLIVTGQVQQVFYRKYTVAKALELGITGYVENRNDGSVHIV
                     ANGNADSLTALENWCYTGSPLSKVIEVKRFENAEVLNENTFVQH"
     gene            complement(430..1170)
                     /locus_tag="PLO59_03680"
     CDS             complement(430..1170)
                     /locus_tag="PLO59_03680"
                     /inference="COORDINATES: protein motif:HMM:NF018473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HQV00233.1"
                     /translation="MIRNFFFLICILLLGYKVAAQTEMQPVAVVELFTSAGCNKCPTA
                     DSVLQKIADEARLQKKNVVTIAYHVDYWNKPYKDPYSKNQFTYLQQNYVSVLQLKEMY
                     TPMMVVNGAQTATGNNFNTGWSLIAKQLQKPANNSLSLTPDSIINDTLYVSYLISKQT
                     KNTSIKILLLENNLHNQIPAGQNKGLTLHYNYVCRIYKAKSIESKKGQIAIALKGLKL
                     NPNISLVGLLQQRGSLQIDAAFKGTLTK"
     gene            complement(1305..>2588)
                     /locus_tag="PLO59_03685"
     CDS             complement(1305..>2588)
                     /locus_tag="PLO59_03685"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HQV00234.1"
                     /translation="KLHVIGSGFFQGNLHVTNNASFGASIGISGDVTATGTVTGNKLA
                     GNTADISGNITMGDTLFIGSVERIIDAGGFTLGCYGDFVPETNAGSSLGQAGRAWSQL
                     HLTSNANIGGNVTVTGSASIGNGINVIAGNISSGGDLYTSSGNGVINCGGDSMSAFIN
                     VLADNFTPGTSNLNANGDEDLYIGGDLEVTLKGYQTGGGAWGVRSDARLKKDVVDFTD
                     GLDALLKIRPVKFKYNNLMPNHTEQEYVGIIAQEMQEIAPYMVEEKPMGQIVEEDANG
                     KEVIVKQGTNYLSYDPNALWYITVNAVKEQQKIIEKQAERIEALEALLKDNATTTHRN
                     TLQTVTSKSTIEQNQPNPFNQSTVINYNLTDDNAKAHIIIRDLSGNMLKKCVLGQRGK
                     GQVVIQANEFAQGTYTYSLEVNGQSIDTKLMVIVN"
BASE COUNT          775 a          493 c          464 g          856 t
ORIGIN      
        1 gaggtacatg atttgtttca taccattgtt gaaaaatgtg cacccaaatc gggcgatagc
       61 cataatcacg aggggcataa tcataattaa atgcagtgta gtttaattgt taccggccag
      121 gtacagcaag tgttttaccg taaatatacc gttgccaaag cactggagtt aggcataaca
      181 ggttatgttg aaaacaggaa cgatggcagt gtgcatattg ttgccaatgg caatgccgac
      241 agtctaactg cattagaaaa ctggtgttat acgggttcgc ccttatcgaa ggtgatagaa
      301 gtaaagcggt ttgaaaatgc ggaagtttta aatgaaaaca catttgtaca acattaaaca
      361 aatagtaact gactaaaaac aaaaagccgg agtaatttaa ctccggcttt ttgtttttcg
      421 caggtttaat tatttagtaa gcgtaccctt aaaagctgca tctatttgca aagagcctcg
      481 ttgttgcaat aaaccaacca gactgatatt gggatttaat tttaaccctt taagtgcaat
      541 tgctatttgc ccctttttag attctatgct ttttgctttg taaatgcggc aaacgtaatt
      601 atagtgtaat gtaagacctt tgttttggcc ggccggtatt tgattatgta agttgttttc
      661 gagtaataaa atttttattg aagtgttttt cgtttgcttg cttattaaat agcttacata
      721 aagtgtatcg tttataatcg aatccggtgt tagtgataag ctattgttgg ccggtttttg
      781 taattgtttt gctatcaaac tccagcctgt gttaaaattg ttacccgttg cagtttgtgc
      841 accattaaca accatcatag gggtgtacat ttcttttaat tgcaagacac ttacatagtt
      901 ttgttgcagg taagtaaact gatttttact gtatgggtct ttataaggtt tgttccaata
      961 atcaacatga taagctatgg ttacaacatt tttcttttgc aaacgtgctt cgtcagcaat
     1021 tttttgcagc accgagtcgg cagtcgggca tttattgcaa cctgctgaag taaaaagctc
     1081 gaccacggct acaggttgca tttcggtttg tgctgcaact ttgtaaccaa gcagtaaaat
     1141 gcaaatcaga aaaaaaaaat tacgaatcat attcaactaa acgaatgctt ctataaaatg
     1201 ttgccatcaa ttaaatgtgg ttataatctt taaaagtgtg tgcaacatag caaaaagggc
     1261 aaacgaaagt ttgccctttt tgaaactaaa atataatcag aaacttaatt tacaattacc
     1321 ataagtttgg tatcaatact ttggccatta acttctaaac tgtatgtata agtgccttgc
     1381 gcaaactcat tagcctgaat aactacctga cctttaccgc gttgtcctaa cacacacttt
     1441 ttaagcatgt tgccgcttaa atcacgtatt atgatatgcg ctttagcatt atcgtcagtt
     1501 aagttgtaat tgataactgt actttgatta aatggattgg gttggttttg ttcgatagtt
     1561 gatttactgg taaccgtttg caatgtattg cgatgcgttg tggtagcatt atcttttaat
     1621 aatgcttcta aagcttcaat acgttcggct tgtttttcaa ttattttttg ttgctcctta
     1681 actgcattta cggtaatata ccataaggca ttagggtcgt atgataaata gttggtgcct
     1741 tgttttacaa taacttcttt accattggcg tcttcttcaa caatttggcc cattggtttt
     1801 tcttcaacca tgtaaggtgc aatttcttgc atttcctgag ctataatacc aacatactct
     1861 tgttcggtat ggttaggcat taagttgtta tacttaaact taacaggacg tatttttaac
     1921 aatgcatcta aaccatcggt aaaatcaact acatcctttt taaggcgtgc atcgctgcgt
     1981 acgccccagg caccaccacc ggtttgatat ccttttaacg ttacttctaa atcgccaccg
     2041 atatataaat cttcatcacc attcgcattt aaattactgg taccgggtgt aaaattatca
     2101 gccaaaacat ttataaaagc cgacattgaa tcaccaccgc agttaataac accatttccg
     2161 gacgaggtat ataaatcgcc acctgacgaa atattgcctg caataacatt gattccatta
     2221 cctatgcttg ctgagcctgt aacagttaca ttaccaccta tatttgcatt tgatgtaaga
     2281 tgcaattggc tccaagctct accagcctgc cctaacgatg aacctgcatt cgtttcagga
     2341 acaaaatcgc cataacagcc aagtgtaaaa ccacctgcat caattattct ttcaacagaa
     2401 ccaataaaaa gtgtatcgcc catggttata ttacctgata tatcagcagt attacctgca
     2461 agtttattac ccgtaacagt acctgtggca gttacatcac ccgatatacc aatgctggca
     2521 ccaaaacttg cattgttagt tacatgtaaa ttgccttgaa aaaaacccga gccaattaca
     2581 tgcagttt
//
LOCUS       DAOMAA010000207         2586 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_34726_length_2586_cov_2.449230, whole genome shotgun sequence.
ACCESSION   DAOMAA010000207 DAOMAA010000000
VERSION     DAOMAA010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34726_length_2586_cov_2.449230"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="PLO59_03690"
     CDS             complement(<1..336)
                     /locus_tag="PLO59_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014782871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HQV00235.1"
                     /translation="MSNLENTQAQMRKGVLEFCILSIISKGEIYPSDIISKMKEAKLI
                     VVEGTLYPLLTRLKNAGLLGYRWIESNAGPPRKYYALTELGQSYLQELNTTWNELVAA
                     VNQTANINTQ"
     gene            complement(558..995)
                     /locus_tag="PLO59_03695"
     CDS             complement(558..995)
                     /locus_tag="PLO59_03695"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HQV00236.1"
                     /translation="MKKITKNSFIKRTSFPGGLKALRQFFDDHLIYPQAAIDAKISGT
                     VTVKFNINYKGVVFDAHLVNSLGFGCDDEALRLVSLLQYNKTLNRGLHVVFHETIHIH
                     FSLQKYLQSKTALQPLQTIVYHYQTEKPKNQTTVIQVNYQVKD"
     gene            complement(1075..1905)
                     /locus_tag="PLO59_03700"
     CDS             complement(1075..1905)
                     /locus_tag="PLO59_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00237.1"
                     /translation="MKSKIKIFQIIVFFVFVSPAYAQETKHASHKPVGDRYVLHAGIF
                     RPYVATSLQINNQYTPGTIISLEDDLGFEDRPWLFKVDGYAAITKRSAFKLTYTNLNR
                     KSAWDIDRDINIFDTTFHVGANLDVFFNTTFLSLSYKYNIFTKPTWDAGAAFGIRFLR
                     IKTGAKLETNNFSKFDESVAIAAPAPVFGLYGNAYLNEQLMFRCSFDYFSVSIKGIRG
                     FVIDNNLSLEYYFIKNLGVGAAVNFLAFRIDDLPIGENFDGQIKYNLSGFSLFLAAKF
                     "
     gene            complement(2056..>2586)
                     /locus_tag="PLO59_03705"
     CDS             complement(2056..>2586)
                     /locus_tag="PLO59_03705"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019942010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HQV00238.1"
                     /translation="DGLNSDLISKGLAEAMKGEKTAVDAQTANQIIQDYMTAAQAKKG
                     AEAVARCGAFLENNKKRKEVTTLPSGLQYEVIKTGTGPKPSATDKVKVHYHGTLINGD
                     VFDSSVERGQPAEFGVNQVIKGWTEALQLMNTGSKWKIFLPADLAYGDRGAGGKIGPN
                     EALIFEVELLEVLKSE"
BASE COUNT          794 a          477 c          447 g          868 t
ORIGIN      
        1 ttgggtattg atgtttgcgg tttggtttac ggcagctacc agttcgttcc aggtagtgtt
       61 taattcttgt aaataacttt gccctaactc ggttagtgca taatacttgc ggggtgggcc
      121 ggcattggat tcaatccaac gatagcctaa caaccctgca tttttcaggc gggttaataa
      181 ggggtataat gtgccttcta ccactatcaa tttagcctct ttcattttcg aaataatgtc
      241 ggacggataa atttcgccct tcgaaataat tgatagaata caaaactcta ataccccttt
      301 ccgcatttgc gcttgtgtgt tttccaaatt gctcatgatg cagttgtttt gggtttatat
      361 tcatttaatt atggtatata gctatacctt atatcttgct ttacaaagat agagttatta
      421 aacagtacct tgttacacat agtactaaat tattttatat tatattgatt ttcagttact
      481 taaatttata agtactgtat taagcaatca aattaaatga ccttttgatt tacataggtt
      541 ataaaaacag caaaaaacta atccttaacc tgataattaa cttgaataac ggttgtttga
      601 tttttaggtt tttcggtttg atagtgatac acaattgttt gcaaaggttg caatgccgtt
      661 ttgctttgta aatacttttg caggctaaaa tgtatgtgta tggtttcatg aaaaactaca
      721 tgtaaaccgc ggtttagtgt cttgttatat tgcagtaaac ttactaaacg caatgcttca
      781 tcatcgcatc caaatcccag gctgtttacc aaatgtgcat caaacacaac acctttgtaa
      841 ttgatattaa atttaacggt tacagtgccg ctaatttttg catcaatggc agcttgtgga
      901 taaattaaat gatcatcgaa aaactggcgc aacgctttaa gacctcccgg aaatgaagta
      961 cgttttataa aactgttttt ggtgattttt ttcatttcga tttacaatgt cgaaatatac
     1021 agtttgttat tggcaacaaa taaattaatg agcagcaatt aaaactaaaa ctgattaaaa
     1081 tttggctgct aaaaaaagac taaatccact gaggttatat tttatctgac catcaaagtt
     1141 ttcgccaatg ggtaagtcat caatacggaa agccagaaaa ttaacagcag cacccacacc
     1201 taaattttta ataaaatagt attcaagcga taagttattg tcgattacaa aacctctgat
     1261 gcctttaatt gaaacagaaa agtaatcaaa actgcatctg aacattaatt gctcatttaa
     1321 gtatgcattg ccatataagc caaaaaccgg tgccggtgct gctatcgcta ctgactcgtc
     1381 aaattttgaa aagttatttg tttcgagctt tgcacctgtt ttaattctga ggaaacgaat
     1441 accaaatgca gcgcctgcat cccaggttgg cttagtaaaa atattatact tataacttag
     1501 cgataaaaag gttgtattaa aaaatacatc caggttagca ccaacatgaa aggtggtgtc
     1561 aaaaatattt atgtcgcggt caatatccca ggccgacttt cggttaaggt ttgtataggt
     1621 taacttaaaa gccgagcgct ttgttatagc agcataacca tcaactttaa aaagccaggg
     1681 tctgtcttca aatcccaaat catcttctaa tgaaattatt gtgcccggtg tatattgatt
     1741 atttatttgc aacgatgtag caacataagg tctgaaaata cctgcatgta atacatacct
     1801 atcgccaact ggcttatgtg atgcatgttt agtttcttgt gcataagcag gcgaaacaaa
     1861 aacgaaaaaa actatgattt gaaaaatttt gatttttgat ttcatcaata aaaagtattt
     1921 caccaaatgt aatattgttg gtaatagtaa tagttcgata tttatctact aaccaattaa
     1981 gcggctaatg gcggtgtacc aataaaaaaa agcctcccta aacaggaggc tttttttata
     2041 ttaacgataa tgcaattact cgcttttcaa tacttcaagt aattcaactt caaaaatcaa
     2101 agcttcgttt ggaccaattt taccaccggc accacggtcg ccataagcta aatcggctgg
     2161 taaaaatatt ttccatttac tgccggtatt catcaattga agggcttccg tccagccttt
     2221 aataacttgg tttacgccaa actcggcagg ttggccacgc tctaccgaac tatcaaacac
     2281 atcgccatta attaatgtgc cgtggtagtg tactttcact ttatcggttg cacttggctt
     2341 ggggccggta ccggttttta ttacttcata ttgcaatcca cttggtaggg tggttacttc
     2401 tttacgtttt ttattgtttt ctaaaaacgc accacaacgt gcaacagctt cggcaccttt
     2461 tttagcttgt gctgctgtca tataatcctg aataatttga ttggctgttt gtgcgtcaac
     2521 agccgttttt tcgcctttca tagcttcggc taatcctttc gaaattaaat cgctgtttaa
     2581 tccgtc
//
LOCUS       DAOMAA010000208         2583 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_34792_length_2583_cov_3.268987, whole genome shotgun sequence.
ACCESSION   DAOMAA010000208 DAOMAA010000000
VERSION     DAOMAA010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34792_length_2583_cov_3.268987"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1251)
                     /locus_tag="PLO59_03710"
     CDS             complement(<1..1251)
                     /locus_tag="PLO59_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00239.1"
                     /translation="MKTYMFIYKIAFVILSIMLSLSTRAQTDTTKTLQRLAIDSNRQK
                     LNMDAVYNRPFLSVGKLPVALGGYLEANTQYQVTDGVSDGFSFQARRLTLFMSSTIAR
                     KIKFLSEIEFEDGTKEINIEFAAMDIEFHPMLNFRGGIIMNPIGAFNQNHDGPRWDFI
                     DRPFVATAIIPTTLASAGFGIHGKHFANNWVLGYETYLTNGFDDHIISNTDGRTSLAA
                     GKINPLRFEESNSGLPMFTGKFAVRNRKIGEAGISYMTGVYNKWKLDDIKIDNKRHAS
                     VLAFDFNTSIFKNRLSLMGELAKVWVVVPDAYTQQYGQQQLGIYLDAIYTVLQRKISD
                     WDNAKLNAGLRIEYADFNQGTFNENGKNIGDHVWAIVPAIAFRPVGSTVLRFNYRFEQ
                     QTDLLGNPPANTAAFQFGFRLIFKL"
     gene            complement(1226..1663)
                     /locus_tag="PLO59_03715"
     CDS             complement(1226..1663)
                     /locus_tag="PLO59_03715"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HQV00240.1"
                     /translation="MNRKDFIKTCSIACIGLTGLVGFQGCSSTNYFAKTIYDGEKLTV
                     KKSEFLIADNAKKSERKFVLIKSESLRFPIIIYKLSDIEYTALLIQCTHNGCELQPQG
                     SYLTCPCHGSEFTNKGVVLNPPAETNLKSYKTTTDHENIYVHL"
     gene            complement(1667..>2583)
                     /locus_tag="PLO59_03720"
     CDS             complement(1667..>2583)
                     /locus_tag="PLO59_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002981201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HQV00241.1"
                     /translation="FGQTDSTGNHYLHQGGPQLQNRALPGFTPEVLPAGASFSKFTAP
                     AVTGLGLLATVSEADLLAMTDPNDIDGDGISGTVNWIDLPLTIAANQNAITQNGKYIG
                     RFGKKASTYNLLQQTITAYNQDMGITSTFDAKDVFSGHDLDPEVSNQTIHDVVFYLHT
                     LKAPIQRNQNDAEVIAGKKLFIQIKCASCHKTELKSGYSPIEVLSNKSFYPYTDLLLH
                     DMGHTLDDAYTEGSAHSYEWRTPALWGLGLSPDAQGGNYFLMHDGRANSIEAAILLHG
                     GEGASSKSLYQNLSANEKAALIKFLESL"
BASE COUNT          750 a          525 c          493 g          815 t
ORIGIN      
        1 taatttaaaa ataagtcgaa aaccaaactg aaatgctgcc gtatttgccg gtgggttacc
       61 taataaatca gtttgttgtt cgaaacggta attaaaacgc aataccgtac tgccaaccgg
      121 cctaaatgca attgcgggta caattgccca tacatggtcg ccaatatttt ttccgttttc
      181 gttaaatgtg ccttgattaa aatcagcata ttcaatacgt aaacccgcat ttaatttggc
      241 attatcccag tctgatattt ttcgttgtag tacggtataa atggcatcca agtaaattcc
      301 taattgttgc tggccatatt gttgtgtata ggcatccggc acaacaaccc aaactttagc
      361 aagttcgccc attaatgata agcggttttt aaatattgag gtattaaaat caaaggctaa
      421 aaccgaagca tgtcttttat tatctatttt aatatcatcg agtttccatt tattgtaaac
      481 accggtcata tacgaaattc ctgcttcgcc tatcttccgg ttgcgaaccg caaatttacc
      541 tgtaaacatg ggcaagccgc tattgctttc ttcaaaacgc agcgggttta tttttcctgc
      601 tgcaagcgat gtgcgtccat ctgtgttcga aataatatgg tcatcaaaac cattggttaa
      661 ataagtttca taacccaata cccagttatt agcaaagtgt ttgccatgta ttccaaatcc
      721 tgcactggca agtgtagtag gaataatagc agtggcaaca aagggtctgt caataaaatc
      781 ccaacgaggg ccatcatgat tctgattaaa tgcgccaatc ggattcataa ttatgccgcc
      841 tctgaaattc agcatgggat gaaactcaat gtccattgca gcaaattcaa tgtttatttc
      901 tttggtgcca tcttcaaact ctatctccga caggaatttt attttgcgcg caatggttga
      961 cgacataaat aaagtaagcc ttctggcttg aaacgagaaa ccatcagaca caccatcggt
     1021 aacttgatat tgtgtatttg cttctaaata accacccagt gcaaccggca acttgccaac
     1081 agataaaaac ggacggttat aaaccgcatc catattcagt ttttgcctat tactgtcaat
     1141 cgctaaacgt tgtaacgttt ttgttgtatc tgtttgtgcc cgggttgata acgacagcat
     1201 tatacttagt atgacaaaag caattttata aatgaacata tatgttttca tggtcggttg
     1261 tggttttgta tgatttcaga ttggtttcgg caggtggatt taataccaca cccttattag
     1321 taaactcact gccatggcaa ggacaagtca agtaactacc ctgaggttgc aactcgcaac
     1381 cgttatgtgt gcattgtata agcaaagcag tgtattcgat atcacttagt ttatagatga
     1441 ttattggaaa ccgtaacgac tctgatttta taagaacaaa tttccgctct gattttttag
     1501 cattatccgc tatcagaaat tctgattttt ttacagttaa tttttcaccg tcatatatgg
     1561 ttttcgcaaa gtaattggta ctgctgcaac cttggaaacc aaccaagcct gttaggccaa
     1621 tacaagcaat gctacatgtt ttaataaaat ctttacggtt cattttttaa agagattcta
     1681 aaaatttaat taatgcagct ttctcattag cggataaatt ttgataaaga cttttgctgc
     1741 ttgctccttc gccaccatgc aatagtatag ctgcttcaat gctgttagca cgtccatcgt
     1801 gcattaaaaa atagttgccg ccttgtgcat cgggcgatag gcccaaaccc cacaatgcag
     1861 gtgtgcgcca ctcataacta tgtgcactgc cttcggtata agcatcatct aaagtatggc
     1921 ccatatcatg caataataaa tcggtgtagg ggtagaatga tttgttagat agtacttcaa
     1981 tgggcgaata ccccgatttt aattccgtct tatgacacga tgcgcatttt atttgtataa
     2041 acagtttttt acccgcaatt acttcggcat cattttgatt gcgttgtatg ggagctttta
     2101 atgtatgcag ataaaacact acatcatgta tcgtttgatt actaacttca gggtctaaat
     2161 cgtgcccgct aaacacatcc ttggcatcga aggtagaggt tatgcccata tcctgattgt
     2221 aagcagtaat ggtttgttgc aacaaattgt acgtagatgc ttttttacca aaacgtccga
     2281 tgtattttcc gttttgtgtt atagcgtttt gattagcagc aattgtaagt ggcagatcaa
     2341 tccagtttac agtgcccgaa ataccatcgc catcaatatc gtttgggtcg gtcatggcta
     2401 ataaatctgc ttctgaaacg gtagctaaca agcccaagcc cgtaacagca ggtgccgtaa
     2461 attttgaaaa actggcgcct gccggaagta cctcaggtgt aaagccgggt aaggcacggt
     2521 tttgtaattg tggaccacct tgatgcaaat aatgattgcc cgtactgtcg gtttgcccaa
     2581 agc
//
LOCUS       DAOMAA010000209         2574 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_35038_length_2574_cov_2.856689, whole genome shotgun sequence.
ACCESSION   DAOMAA010000209 DAOMAA010000000
VERSION     DAOMAA010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35038_length_2574_cov_2.856689"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..1044)
                     /locus_tag="PLO59_03725"
     CDS             complement(52..1044)
                     /locus_tag="PLO59_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HQV00242.1"
                     /translation="MKVIIPVAGIGSKLRPHTHTQPKALVPVAGKAILSHIVDFLLHS
                     GCTEFIFIIGYMGDKIELHVKEKYPKLKATFILQEPRQGTGHAVYMAHACIKKKEGVF
                     IVFGDTIFDYDLRALIKEGVSSLAVKKVEDPRQYGVVELDENGYVTNLQEKPKIPKSN
                     LALTGLYYIKQASELMNCMKAQIEANEKNNDEFSLTAGLMAMIAQGQKFKTFQTGSWF
                     DCGSRISLLETNATLLKRIKKKKTSYHYHNTIIIEPVSIADDCNIRNSIIGPNVSVGE
                     KTIIEKSIVRDSIIGSYSQIETAILHESVVGSDAYLKGLSQSLNIGDSTEIDFS"
     gene            1252..2199
                     /locus_tag="PLO59_03730"
     CDS             1252..2199
                     /locus_tag="PLO59_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008240459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HQV00243.1"
                     /translation="MNNICKLFNISYPIVQAGMIWCSGWRLASAVSNAGGLGIIGAGS
                     MYPDVLKLHIEKCKAATQKPFGVNVPLLYPDVDALIQIIIEQKVEVVITSAGNPKTWT
                     QHLKQHGIKVVHVVSNTKFALKSVEAGVDAIVAEGFEAGGHNGREETTTMCLIPMIVS
                     AVTVPVIAAGGIGSGRAMLAAFALGAQGVQVGSRFVNAIESSAHDHFKQAIINSKEGD
                     TQLLLKKLTPVRLLRNAFQQRVIDAENTCADTLTLTEILGRARAKKGMFEGDMIEGEL
                     EIGQVSAAINETLPAATIVKNMMLEFDAARVQMDALKFI"
     gene            complement(2351..2545)
                     /locus_tag="PLO59_03735"
     CDS             complement(2351..2545)
                     /locus_tag="PLO59_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015697087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HQV00244.1"
                     /translation="MSERQGVEIDYCPTCRGVWLDRGELDKIIDKSFAHDSGQAHYQK
                     QTHYSDHKYKKKSWMHDLFD"
BASE COUNT          755 a          467 c          500 g          852 t
ORIGIN      
        1 taagcttaaa aattgctgtg tctttggttt tcatatatga tgtggattgt attatgagaa
       61 atcaatttct gtactgtcgc caatgttcag gctttgcgat aatcctttaa ggtatgcatc
      121 gctgccaacc acactttcgt gtaatatggc tgtttctatt tgcgagtatg aaccaataat
      181 gctatcacgc acaattgatt tttcgatgat ggttttttcg ccaaccgaaa catttgggcc
      241 aattatgctg ttacgaatat tgcaatcatc ggcaatactt acaggttcaa ttataatggt
      301 attatgatag tgatagctgg tttttttctt tttaatgcgt ttaagcaaag ttgcattggt
      361 ttctaacaaa ctgatgcggc tgccgcaatc gaaccaactg ccggtttgaa acgttttaaa
      421 tttttggcct tgtgcaatca tggccataag gcctgctgtt aaactaaact catcgttgtt
      481 tttttcgttg gcttcgattt gggctttcat acaattcatt aattcggagg cttgtttaat
      541 ataatacaag cccgttaatg ccaaatttga tttgggtatt ttgggttttt cctgaaggtt
      601 ggttacatag ccattttcat ctaattcaac cacaccatat tggcgtgggt cttcaacttt
      661 ttttaccgcc aggctcgata caccttcttt aattaatgca cgcaaatcat aatcaaatat
      721 cgtatcgcca aaaacaataa acacaccttc tttcttttta atacatgcat gcgccatata
      781 aacagcatgg cccgtacctt gccgtggttc ttgtaaaata aaagtggctt ttagtttggg
      841 gtatttttct ttgacgtgta attcaatctt atcacccatg tatcctatta taaatataaa
      901 ctccgtgcaa ccgctatgta ataagaaatc aacaatgtgt gataatattg ctttacccgc
      961 tacaggtacc aatgccttag gttgtgtatg cgtatgcggc ctgagtttac ttccgatacc
     1021 ggccacagga ataataactt tcaaaaatta tggcgttttt aatgatgggg cagcaaagct
     1081 aaactaattg ctataacagc cataactttt aataattgga ctcaaagcat tttatagatt
     1141 ataattttgg tttcggctta attagtcatg caaagcaact tttaaaaagt tgataacaac
     1201 acggcttgct tcgtatttca attcaatagt attgcctctt aaatttttta tatgaataac
     1261 atttgtaaac tatttaatat ttcatatccg atagtacaag ccggcatgat ttggtgtagc
     1321 ggatggcgat tggcatcagc cgtaagtaat gccggtggtc tgggtataat tggagctggg
     1381 agtatgtacc ccgatgtatt aaaattacat atcgaaaaat gtaaagcagc cacacaaaaa
     1441 ccatttggcg ttaatgttcc attgctttat cctgatgtgg atgcacttat tcaaataatt
     1501 attgaacaaa aagttgaagt agtaattaca tcggcaggca atccaaaaac atggacacaa
     1561 catttaaagc aacatggcat taaagtggtg catgtagtaa gcaataccaa atttgcgcta
     1621 aaaagtgttg aggctggtgt tgatgctatt gttgccgaag gttttgaagc cggtggccac
     1681 aatggccgcg aagaaacaac taccatgtgc ttaattccta tgattgtatc ggctgttact
     1741 gtacccgtta ttgctgcagg tggtattggt agtggccggg ctatgttggc tgcttttgct
     1801 ttgggcgcac aaggtgtgca ggtgggtagt cgttttgtaa atgcaattga atcgtcagca
     1861 catgatcatt ttaaacaagc cattataaat tcgaaagaag gcgacaccca attgttgtta
     1921 aaaaaactaa cccctgtaag gcttttacgc aatgcatttc aacaacgtgt gattgatgct
     1981 gaaaacacct gtgccgatac attaacacta accgaaatat taggccgtgc acgtgcaaaa
     2041 aagggtatgt ttgaaggtga tatgattgaa ggcgaattag aaattggcca ggtaagtgct
     2101 gcaattaatg aaacactacc tgctgcaacc attgttaaaa atatgatgct tgaatttgat
     2161 gctgcacgtg tacaaatgga tgcattgaaa tttatttaat cttatttttt tgccaaaaga
     2221 tatcgatctt atagcgcttg cttaactgta aactcagtgg tactttggaa ttttaatgca
     2281 agtacaaacc catatgttta cttactaaaa atgaggctga tatgcctcat ttttaaaacg
     2341 ctacaatttg ttagtcaaac aaatcatgca tccaactttt ctttttgtat ttgtggtcgc
     2401 tgtaatgggt ttgtttttga taatgtgcct gtcctgaatc gtgggcaaat gatttgtcaa
     2461 ttattttatc cagttcacct ctgtcgagcc aaacaccacg acaagtaggg caataatcta
     2521 tttcaacacc ctgacgttca gacattacta aatcaacggt tgtacaattc ggac
//
LOCUS       DAOMAA010000210         2569 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_35170_length_2569_cov_2.773667, whole genome shotgun sequence.
ACCESSION   DAOMAA010000210 DAOMAA010000000
VERSION     DAOMAA010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35170_length_2569_cov_2.773667"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1334)
                     /locus_tag="PLO59_03740"
     CDS             complement(<1..1334)
                     /locus_tag="PLO59_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00245.1"
                     /translation="MKNIITTVKAKVFCQMPAVYFTVLLLANVCSLPLYGQVPVAGFG
                     TTQADIKMGDTVNFVNTSIGFTNGTLFVWNFGLPCDTDALGNPVVAACNDTVTGQISV
                     FNIYKLPGNYTVTLKALHNGNVYTTFQTIYIKASLDPACVDLNPCNLISNADFETVST
                     CPGTDYQFLGTGPFVWGNPNPPASCNWLYPTYGSPDLFFHNTNTPACPNSNWAANSGH
                     LNSQTMAGGTILGGSNYREYIVQELKHALVPNKIYHLRLWTRLWTNINTTECKLQVAF
                     TNSNIADYGTINPLPLPANALILTSANRLTNDNLWHAVDYTFTTTQVGLNQIIIGNFE
                     NDNSPTLDAANSSGYVFPFYNDITLFAVNGLDANVENHCNGMGSISINAPCNTPTQTF
                     TPPFTYWWSNGSTLQNQALLTPATYTVTVTDANGATLSESFTVLATAVVTPP"
     gene            1696..2421
                     /gene="recO"
                     /locus_tag="PLO59_03745"
     CDS             1696..2421
                     /gene="recO"
                     /locus_tag="PLO59_03745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HQV00246.1"
                     /translation="MIIKTQGIVMHTIAYSETSVIAKIYTAQLGMQSYMLKGVRKPKS
                     RINMAMLQPLTLIEFVSNHKPNKTLHFITELSTAPAYQTIPYDVNKSTIAFFLAEVLG
                     KSIREEEANTQLFQFIRQHLLWLDANAANCKQFHHYFLVQLTQHLGFFPDGSFDTGHT
                     FFDLREGVFLFQMPLHPDFIAADDARLLHFFMQSNLQTHQQITTTHTQRKTLLTHVLS
                     YYYHHNLYGSTIKTHQILEEIMA"
BASE COUNT          807 a          508 c          480 g          774 t
ORIGIN      
        1 tcgggggggg ttaccacagc agttgctaaa accgtaaaac tttcggatag ggttgcacca
       61 ttggcatcag taactgttac tgtataggtg gccggggtta gcaatgcctg attttgcaaa
      121 gtactgccat tactccacca ataagtaaat ggtggcgtaa atgtttgtgt aggtgtattg
      181 catggggcat taatactaat actgcccatg ccgttgcaat ggttttcaac gtttgcatct
      241 aagccgttta cggcaaataa ggtaatgtcg ttataaaatg ggaaaacata tccgctactg
      301 ttggcagcat ctaaggtggg gctattatcg ttttcaaaat taccaataat aatttggttt
      361 aaacctactt gtgttgtagt aaaagtataa tccacagcat gccataggtt atcgttagtt
      421 aagcggttag ctgatgttaa tataagtgca ttagcaggca gtggtagtgg attgattgtg
      481 ccataatcgg caatgttgct gtttgtaaat gctacctgca gtttacattc cgtagtgtta
      541 atgttggtcc aaaggcgagt ccataatcgt aaatgataaa ttttatttgg caccaatgca
      601 tgctttaatt cctgtacaat atattcccgg taattgctgc cgcccaaaat tgtgccccca
      661 gccatagtct ggctgtttaa atggccacta ttggccgccc agttactgtt tgggcaagcc
      721 ggtgtattgg tattgtgaaa aaataaatcg ggcgagccat aagttggata caaccaattg
      781 cacgatgctg gtgggttggg attaccccac acaaaaggcc ctgtacctaa aaattgataa
      841 tcggtgcccg ggcaagtgct aaccgtttca aaatcggcat tgctaattaa attacaaggg
      901 tttaagtcaa cacaggcggg atctaaagaa gcttttatat aaatagtttg aaaagtggta
      961 tatacatttc cgttgtgtaa tgctttaagt gtaactgtat agttgccggg taacttataa
     1021 atattaaaca cgctaatttg cccggtaacg gtatcgttac aggctgctac cacagggttg
     1081 ccaagggcat cggtatcgca gggtaagcca aaattccaaa caaacaaggt cccgttagta
     1141 aagcctattg aggtgtttac aaagtttaca gtatcgccca tttttatatc ggcttgcgta
     1201 gttccaaaac ctgcaacagg cacttgccca tataaaggca aactgcaaac atttgctaaa
     1261 agcaaaacag taaaataaac agctggcatt tggcaaaaaa ctttggcttt tacagtggta
     1321 attatatttt tcatacgcag catattttag gtgataactt ctatgaaggt atcctttaag
     1381 ttattaccaa atactttttt atagaagttg cctgaaacat ctatagttaa aataataaac
     1441 gagttgggtg tatcaattta tttcggggcc tgttactaat caactatttg taaacatcac
     1501 aacaaccaaa gctcaaagag taagcatgct tttattcgtt atgccgtatt tacgcttgct
     1561 tttcaaaaac agctacagaa aggctttcaa ttgtttaccc actaaacagg ttataatatt
     1621 taaacgtttg ttttagtatt tcctgtttat attgtatgta acctaatttt aaatttgcca
     1681 tctaagtttt tcaaagtgat tataaaaaca caaggcattg taatgcatac catagcctac
     1741 tccgaaacat cggttattgc aaaaatatat acagcgcaat tgggtatgca atcgtatatg
     1801 ctaaagggtg tacgtaagcc caaatcgcgc atcaacatgg ctatgctgca accacttaca
     1861 ttaattgaat ttgtatcgaa ccacaaaccc aataaaacat tacattttat aacagaatta
     1921 agtacagcac ctgcctacca aaccatacct tatgatgtaa acaaaagcac catcgctttt
     1981 tttttagccg aagttttagg caaatccata cgtgaggaag aagccaatac ccaactgttt
     2041 caatttataa ggcaacactt gttgtggctc gatgcaaatg ctgcaaattg caaacaattc
     2101 caccactact tcttggtgca gttaacacag catttaggtt tttttccgga tggcagcttc
     2161 gataccggcc atactttttt cgatttgcgc gaaggtgttt tcttatttca aatgccactc
     2221 catcccgatt ttattgctgc tgatgatgcc cgtttactgc atttctttat gcaatcaaac
     2281 ctgcaaacac atcaacaaat aacaactacc catacacaac gcaaaacctt gctaacacat
     2341 gtattaagtt actactacca ccataactta tatggcagta ccattaaaac acatcaaatt
     2401 ttagaagaga taatggcctg agttgctgac attagcccgt taactaatac cgtacaacag
     2461 caacaaataa tttaagcaca taataacgta gcaaacagct atacagccca actgcaaaag
     2521 gcttttatta tctttacgcc ttaattaaaa aacacttgcc atgagcgat
//
LOCUS       DAOMAA010000211         2561 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_35381_length_2561_cov_3.075818, whole genome shotgun sequence.
ACCESSION   DAOMAA010000211 DAOMAA010000000
VERSION     DAOMAA010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35381_length_2561_cov_3.075818"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2215)
                     /locus_tag="PLO59_03750"
     CDS             complement(<1..2215)
                     /locus_tag="PLO59_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00247.1"
                     /translation="MKKLLLIGLLSYISIYTKAQCTGGTANGSITTTAAWQTTGTTNI
                     NGGDYKVFTAVAGYSYEFSFCAADGGSTTFDTQLSILNDATGTAMAGGYNDDFCGLAS
                     SLSWLCPASGNYRILPTRYSCLTQNNMGTMAYRHVAPLTCPSGLGAGVTNVSSLPYAS
                     GAGTTCGLINNFTAANTIACGSNLYLGGEDAVWVFTPAITGSVTMNLDAPSATYTGFM
                     LYQGCPFNGQGGVCVVNNNSSTGSKNMSACLQAGITYYLILDSWPAPTCNAYNNVTIS
                     APVPPGGCTLGTGQVNVTLPYNATGRTTCGMVNDLTAANTFACGSNTYLSGEDEVFVF
                     TPTVTGSVTITITSNSTYVGLMLYQGCPYTGGCLMGTPTCLDFDQSTGGNVTVCANVT
                     AGITYYALLDQYAPPNCIGSYNINITAPVSVSTGATCASALNVTLPYNANNQTTQCLG
                     NDYTNATSGSCGTLYESGNDKVYLYQATAPTCLIVSLSQASNNAIGFQVYQGCPGSGG
                     LCLGSYGGATSGNLSGTVTLPAAGNYFIIVDSWAPPTVVTYEINIVNGGSGAVNDLPC
                     NANVLTLGVYTNGSNNCSGGIGEPAAPACWVAGGNQLNTVWYRFIPTSAQVTIRTTPG
                     TLLNSHIALYSGACNSLTYVACNDNAPSCGTTVNNMSQININTLTPGATYHLVVDGYG
                     GATGTFGIVVIDGAPSNLPPVFGQECVVPNPVCNQIISVGNPGYQAFGSSCDFPGS"
     gene            complement(2218..>2561)
                     /locus_tag="PLO59_03755"
     CDS             complement(2218..>2561)
                     /locus_tag="PLO59_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00248.1"
                     /translation="MRFVFTISLFFFLQSFCSAQFREVILYMPHNDLVKQADSIQNSI
                     SHLQGINCVGVCARFNVAMFKLQVQFHENNDVFFEALKQKHWTYYIKADATIDQVLNA
                     CQLQPLSSRLD"
BASE COUNT          771 a          572 c          514 g          704 t
ORIGIN      
        1 cgcttcccgg aaaatcgcat gacgaaccaa atgcctgata acccggatta cctacggaaa
       61 taatttgatt acaaaccgga ttaggtacta cacactcttg cccaaatacc gggggtaaat
      121 tagacggggc tccatcaatt accacaatac caaaagtgcc ggtagcacct ccatatccat
      181 caacaactaa atgatacgtt gcgcccgggg ttaaggtgtt gatatttatt tgcgacatat
      241 tatttaccgt agtgccgcat gatggtgcat tgtcgttaca agctacatag gtaaggctat
      301 tacaagcacc actatataat gcaatatgag aattaagtag tgtaccgggt gtagtacgta
      361 tggtaacttg tgccgatgtg ggtataaacc tataccaaac agtatttaat tgattaccgc
      421 ctgcaaccca acaagcaggg gctgccggct cgccaatacc acctgagcaa ttgttacttc
      481 cattggtgta aacacccaag gttaacacgt tggcattaca aggtaaatcg ttaacagcac
      541 ccgaacctcc atttacaata ttaatttcat aagtaaccac agttggtggc gcccagctat
      601 caactattat aaaataattt cctgctgccg gtaatgtaac cgtacctgat aaattacccg
      661 atgttgcacc accataactt cccaaacaaa ggcctccact gcccgggcaa ccctgataaa
      721 cctgaaagcc aatggcattg ttacttgcct ggcttaaact cacaattaaa caagttggtg
      781 ctgttgcctg atataagtaa actttatcgt tacccgattc atataaagta ccacaagaac
      841 ctgaagttgc attggtataa tcgttaccta aacattgcgt ggtttggttg ttggcattat
      901 aaggcaaagt aacatttagt gctgatgcac atgtagcacc tgtacttacg gataccggtg
      961 ccgttatatt gatgttataa ctaccaatac aattgggtgg tgcatattga tctaacaatg
     1021 cataatatgt tattcctgca gttacattgg cacataccgt tacattacca ccggtgcttt
     1081 ggtcaaaatc caaacaggta ggtgttccca ttaaacaacc tccggtatag gggcaaccct
     1141 gataaagcat taagccaaca tacgtactgt tacttgttat ggtaatggta accgaccctg
     1201 ttacagttgg tgtaaataca aacacctcgt cttcgccact taggtatgtg ttcgaaccac
     1261 atgcaaatgt attggctgct gttaaatcgt taaccatgcc acaagtggtg cggcctgtag
     1321 cattgtaagg cagcgtaaca tttacctgcc cggttcctaa agtacagcca cccggaggca
     1381 ccggtgccga tatggtaaca ttattatatg cattgcaagt tggtgccggc cacgaatcta
     1441 aaatcaaata gtaagtgatg ccggcttgta aacaagccga catgttttta ctgcccgtac
     1501 ttgagttgtt atttaccaca caaacaccac cttgcccatt aaacggacaa ccctgatata
     1561 acataaaacc ggtgtaagta gccgatggtg catctaaatt catggttaca ctacctgtta
     1621 tggccggtgt aaaaacccaa acagcatctt cgcctcctaa atataaattt gagccacatg
     1681 caatggtatt tgcagcagta aagttgttta tcaaaccgca agttgtaccc gctccacttg
     1741 cataaggcaa gctacttaca ttggtaacac cggctcctaa accactgggg catgttaaag
     1801 gcgctacatg gcgatatgcc attgtaccca tattgttttg ggttaagcaa ctatagcgcg
     1861 taggcaaaat tcggtagtta ccactggctg gacaaagcca tgatagtgat gaggccaaac
     1921 cgcaaaaatc gtcattatac cctccggcca tagcggtgcc tgttgcatca tttaaaatgg
     1981 agagctgcgt atcaaatgtg gtactgccac catcggcagc acaaaacgaa aactcataac
     2041 tataaccggc taccgctgta aacactttgt agtcgccacc gttaatgttt gtagtgcctg
     2101 ttgtttgcca ggctgctgtg gtagtaatgg agccattggc agtgccaccg gtgcattggg
     2161 cttttgtata tatgcttatg taacttaata atccaataag taataacttc ttcataatta
     2221 atcgaggcgg ctgctaagtg gttgtaattg acatgcatta agtacctgat ctatagttgc
     2281 atcagctttg atataatacg tccaatgttt ttgttttaat gcctcaaaaa aaacatcgtt
     2341 gttttcatga aactgaactt gtaatttgaa catggccaca ttaaaccgtg cacaaacccc
     2401 cacacagttt ataccttgta aatggctgat actgttttgt atcgaatcgg cttgctttac
     2461 taagtcatta tggggcatgt acaatataac ttcgcgaaac tgggcactac aaaaagattg
     2521 taaaaagaaa aagagggaga ttgtaaaaac aaatcgcata g
//
LOCUS       DAOMAA010000212         2557 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_35493_length_2557_cov_2.181455, whole genome shotgun sequence.
ACCESSION   DAOMAA010000212 DAOMAA010000000
VERSION     DAOMAA010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35493_length_2557_cov_2.181455"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1396)
                     /locus_tag="PLO59_03760"
     CDS             complement(<1..1396)
                     /locus_tag="PLO59_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00249.1"
                     /translation="MHKISTILMICLCSIISNAQNNYVKIFSDNYGILDICENPDSTI
                     SISGIGFDTIYNSGQPSDEGIIGVQFAKVDWFGNLIYRRVYGSLRDEMAGHICRVEEN
                     GNVNYIMSGQTWSFSPPSPAPPSYSQAYLVKVNSNGDTLWTRAVDYGGHVNYQDLTIA
                     TDSNYVYCGSNGAATLTKVNKTDGDTLWTRQYIQPISGSIGSTGLHLAPDSGFYMGGL
                     VIPPAYNKSYFLLLKTDVVGMLQWCKAYGNNLKPMICWDMTVLSTQEVLLVGQTDVTL
                     KSDFGVIKLNALGDTLWCYKYNFLPDQYDVGVSVMEDTDGNYLILGYTNDNTGASKLL
                     KIDTAGNVLWAWRYKWNGGPHLKIKKAYDGGFWIASGVLVKLDSAGYGCTALAHPVSR
                     KAFALNIETINLQMAGGAVACFRTPTIEFTDTASLTDYCIYLGQNDFTFTNKSVHMHP
                     NPASTQLTVQVPNAN"
     gene            complement(1410..>2557)
                     /locus_tag="PLO59_03765"
     CDS             complement(1410..>2557)
                     /locus_tag="PLO59_03765"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00250.1"
                     /translation="GATGAGCYDMVCDASNNLYVVGVIGSSTTGFPFYNPGNGAFFSN
                     ETGSYLAVFSPTSNQKWCTSFKVGQQLRSVDINHADGTILVGGFTTAGSLFPWQNVPV
                     PNSTFFDNTYNGGAFDGFVALINPDYKIYYASILGGNGDEIIQKVAISNTGRIFVAGN
                     THTNGLPAFSNFPTITPHANAYFESSLANNLPNDIFISHLETRSALTPLKLTWSTFFG
                     DDAGHEFVRDINLLGKYLFLTGWTFLEPASPSFPLVNEIGGFLQHNNNPVVAGFNQSG
                     SNGLILKFMNEEGANLRAGKTFENKISISPNPSQADIVNINANFEMRGHAQISINDLT
                     SNCIKLYNINLNEQPLQINTSGIETGYYLITIRTTNFSQSFPFVKIN"
BASE COUNT          786 a          511 c          460 g          800 t
ORIGIN      
        1 tgtttgcatt aggcacctgc actgtaagct gtgtgctggc cgggttgggg tgcatatgaa
       61 ctgacttatt tgtaaacgta aaatcatttt gtcctaaata aatgcaataa tccgttaacg
      121 atgctgtgtc ggtaaattca atggtaggtg tgcgaaagca ggcaactgct cctcctgcca
      181 tttgtaggtt tatggtttca atatttaatg caaatgcttt gcgcgaaacc ggatgtgcta
      241 aagctgtgca gccataacct gccgaatcta attttaccaa tacacctgat gctatccaaa
      301 aaccaccgtc ataggctttt tttattttaa gatgcggacc gccattccac ttataccgcc
      361 aagcccaaag tacattgcct gctgtatcaa tttttaaaag cttagatgca cccgtattat
      421 cgtttgtgta acctaatatt aaataatttc catcggtatc ttccataaca gaaactccaa
      481 catcatattg gtcgggtaaa aagttgtact tataacacca aagcgtatcg cccagagcat
      541 taagttttat taccccaaaa tctgatttta aagttacatc ggtttggcct accaataaaa
      601 cttcctgcgt tgataaaaca gtcatgtccc aacaaatcat gggttttagg ttgttgccat
      661 aagctttgca ccattgaagc ataccaacca catcggtttt caataataaa aaatacgatt
      721 tgttatatgc cggaggtatt acaaggccac ccatataaaa tcctgaatcg ggggcaaggt
      781 gtaatccagt actgccaata gagcccgata tgggttgtat gtattgccgc gtccatagcg
      841 tatcgccatc ggttttattt actttggtta atgtggcagc gccatttgag ccacaataca
      901 cataattgct atcggttgca attgttaaat cctgataatt aacatgacca ccataatcaa
      961 cggcacgtgt ccaaagtgta tcaccattgc tatttacttt taccaaatat gcctgactgt
     1021 aactgggagg tgcaggtgat ggtggagaaa agctccaggt ttggcccgac ataatgtaat
     1081 ttacattacc gttttcttca acacgacaaa tatggcctgc catttcatcg cgtagcgaac
     1141 catacaccct gcgataaatt aaattgccaa accaatctac ttttgcaaat tgcactccaa
     1201 taataccttc atcggatggc tggcctgagt tatagattgt gtcaaatcca ataccagaaa
     1261 tgctaatagt tgaatccgga ttctcacata tatctaatat tccataattg tcagaaaata
     1321 tttttacata gttgttttgt gcattagaaa taatagaaca taagcaaatc attaaaatag
     1381 ttgatatttt atgcataacc attacctcct taattaattt ttacaaatgg gaaactttgc
     1441 gaaaaatttg tggttctaat tgttatcaag taatatccgg tttctatacc cgaagtattt
     1501 atttgtaacg gttgctcgtt taagtttata ttataaagct taatacaatt gctcgttaaa
     1561 tcatttatgc taatttgtgc atggcctctc atttcaaaat ttgcgtttat gttaacaata
     1621 tctgcttggc ttgggttagg ggatattgaa attttatttt cgaaggtttt acccgctctt
     1681 aaattagccc cttcttcatt cataaatttt aatatcaatc cattactacc actttgatta
     1741 aagcctgcaa ctacagggtt gttgttgtgt tgtaaaaagc cacctatttc gtttactaat
     1801 ggaaaactgg gtgaggcagg ttctaaaaat gtccatcctg ttaagaaaag atacttacct
     1861 aataggttaa tgtcccttac aaattcatgg cctgcatcat cgccaaaaaa tgtgctccac
     1921 gttaatttta gtggcgttaa tgcactgcgc gtttctaaat gtgaaataaa aatgtcgttg
     1981 ggtaagttgt tggctaaaga tgactcaaaa taggcatttg catgaggtgt aatggtagga
     2041 aagtttgaaa aagcaggcaa tccgtttgtg tgagtattac ctgccacaaa tattcgtccg
     2101 gtattactga tggctacttt ttgaatgatt tcatcgccat tgccgcctaa tatggaggca
     2161 taatatattt tataatccgg attaataagt gccacaaaac catcaaaagc acccccatta
     2221 taagtattat caaaaaaggt gctattaggt acaggtacat tttgccaggg gaataatgaa
     2281 cccgctgtgg taaatccacc tactaaaatg gtaccatcgg catgattaat atcaacactc
     2341 ctgagttgtt gtccaacttt aaaacttgta caccatttct gattactggt tggactaaaa
     2401 acagccaaat aactacctgt ttcattacta aaaaaagcac cattgcccgg attataaaat
     2461 gggaaaccgg tagttgaaga accaatcaca ccaaccacat ataagttgtt ggaagcatcg
     2521 caaaccatat cgtaacaacc ggcacccgtt gcccctg
//
LOCUS       DAOMAA010000213         2550 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_35698_length_2550_cov_2.472946, whole genome shotgun sequence.
ACCESSION   DAOMAA010000213 DAOMAA010000000
VERSION     DAOMAA010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35698_length_2550_cov_2.472946"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1013)
                     /locus_tag="PLO59_03770"
     CDS             complement(<1..1013)
                     /locus_tag="PLO59_03770"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - viral entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HQV00251.1"
                     /translation="MRKNLINSVITIVCMMFVLPASAQWVRNNVTGVTSLQFPNDKVG
                     IGTPAPTYALDIKQAANCSFRIQSTTSGSSNLILSRSLASTNCLVNYKTGLTDLWQTG
                     CFSGNDNFRIKDGPGNVRLHINQTYGFTGLNSDRINNPPASKTDNSLTIGNLGALYYP
                     GINLYSDATAEYTIIYGITSGLSIRGIQVADETGAFAEVNASAFNVASDRRMKKEIVD
                     ITRDQYPKYMNYIRNIESATFRYNHETEATRKVPHIGVIAQSLPAELQAQIFEKPGQV
                     SDLRLGVSLAEMQGLLLVGIKSLDNDVQHLNESNISLNVLCQQLQFENTNLKARLEIV
                     EQTL"
     gene            1396..>2550
                     /gene="hisS"
                     /locus_tag="PLO59_03775"
     CDS             1396..>2550
                     /gene="hisS"
                     /locus_tag="PLO59_03775"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008151426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HQV00252.1"
                     /translation="MRDFSPSEMVKRQYIFETIKKVYNRFGYQPIETPAMENTETLLG
                     KYGDEGDKLLFRVLDNGNPLNQITSAELAARFGASLFDSAAFVNHISGKGLRYDLTVP
                     FARYVVMHQNDITFPFKRYQIQPVWRADRPQKGRYREFYQCDADVIGSNSLLNEFELI
                     QMIDEVFTALGLKVLIQLNNRKILQGIAEYIGETEKIIEITVAIDKLDKIGLDQVNNE
                     LRSKGISETAIEKLQPVIQLKGTTTEKINALKNILAQSPVGLKGIEELTYIFDLLQQL
                     PLQSAQYELELTLARGLNYYTGAIIEVKVNDLRTDFSSSVLGGGRYDDLTGIFGLPNV
                     SGVGISFGADRIYDVLNALNLFPENVAATTKLLFVNFGSNEEKYCLQLLGK"
BASE COUNT          811 a          481 c          471 g          787 t
ORIGIN      
        1 agtgtttgtt caacaatttc taaacgtgct tttaggtttg tattttcaaa ttgtaattgc
       61 tgacaaagca cgtttaagga aatattcgat tcgtttaaat gctgaacgtc attatctaac
      121 gacttaatac ccaccaacaa taagccctgc atttcggcca aacttacgcc caaacgtaag
      181 tcggaaacct gaccgggttt ttcaaaaatt tgtgcttgca actcggcagg taacgattgt
      241 gctattacac caatatgtgg caccttgcgt gtagcctcgg tttcatggtt atatcggaac
      301 gtagccgact caatgttgcg gatataattc atatacttgg ggtactggtc ccttgtaata
      361 tccactatct cttttttcat acgtctgtcg ctggctacat taaatgcact tgcatttact
      421 tcggcaaatg cgcctgtttc atcggcaacc tgtatgcccc ttatagacaa accggaagta
      481 atgccataaa taatagtata ctctgccgta gcatctgagt ataaattaat gccggggtag
      541 tacaaagcac ctaaattacc tatggttaaa ctattatccg ttttgctggc aggtgggttg
      601 tttatacgat ctgaatttaa tcctgtaaat ccataagttt gattgatatg taaccgaaca
      661 tttcccgggc catcttttat tctgaaatta tcattaccac taaaacatcc ggtttgccat
      721 aaatcggtta ggccggtttt gtagtttacc aaacagtttg tcgaagccaa cgaccttgat
      781 aatattaaat tagagctgcc cgaagttgta ctttgtatgc gaaaactaca gttagccgcc
      841 tgcttaatat ctaaagcata ggtaggcgct ggtgtgccaa ttcctacttt atcattcgga
      901 aactgcaggc tggttacacc ggttacattg tttctaaccc attgtgctga tgcaggcaaa
      961 acaaacatca tacaaacaat agtaattacg gaattaatta gattttttct cataacatta
     1021 ggttttaagt gaataaataa attggagcat tgcaggcatt tcattttaca acttactaaa
     1081 aatgccatac atgtttaaca caatatataa tcagaaaaaa taaaaatgca acctgctttt
     1141 gatgaaacaa atcaattttt gggttaagtg tctgaagtga tttgaagaat aaatatttct
     1201 gacaggtact tgaaatatta tatatcacta ctgattaaat ttaaacggca ataaaatctt
     1261 tagcctaatt gatattgtca tctacaacaa aatagaaaaa tggagtattt aatttaaaat
     1321 tgcagccgca tttaaagcaa ctctttttac acattacctt aattacaaca cttgaaacca
     1381 gcaataccta aaggcatgcg cgatttttca ccgtcagaaa tggtgaaacg tcaatatatt
     1441 tttgaaacca ttaaaaaggt ttacaaccgg tttggctacc aacccataga aacgcctgct
     1501 atggaaaaca ccgaaaccct tttaggcaaa tatggtgacg agggcgacaa acttttattc
     1561 agagtattgg acaatggcaa cccgctcaac caaatcacat cagccgagtt agcggcaagg
     1621 tttggcgcat cgctttttga ttctgctgca tttgtaaatc acatttcggg taaaggctta
     1681 cgttacgatt taacggtacc ctttgctcgt tacgtggtaa tgcatcaaaa cgacattacc
     1741 tttccgttta aacgctatca gatacaacct gtatggcggg ccgataggcc acaaaaagga
     1801 cgctaccgcg aattttatca atgcgatgcc gatgtaattg gcagtaactc gcttttaaat
     1861 gaatttgagc taattcaaat gattgatgag gtttttactg ccttgggatt aaaagtatta
     1921 atccaactta acaaccgtaa aatattgcaa ggcatagccg aatacattgg cgaaaccgaa
     1981 aaaattattg agataacagt tgctattgat aagctcgata aaattgggct tgaccaggta
     2041 aataatgaat tgcgcagtaa aggcatttcc gaaacagcta ttgaaaaact gcaaccggtt
     2101 attcaattaa aaggcactac aactgaaaaa attaatgcac ttaaaaatat tttagctcaa
     2161 tcgcctgtgg gccttaaagg tattgaagag ctaacttata tttttgattt gttgcagcaa
     2221 ctacctttgc aaagtgcaca atatgaatta gaacttacgc tggcacgcgg attaaattat
     2281 tacacaggtg ccattattga agttaaagta aatgatttac gaacagactt tagcagcagt
     2341 gttttaggcg gtggccgcta tgatgacctt acaggtattt ttggcttacc caatgtatcg
     2401 ggcgtaggta tttcttttgg tgccgataga atttatgatg ttttaaatgc cttaaatctt
     2461 tttccggaaa atgtagcggc aactaccaaa cttttatttg taaactttgg cagtaatgaa
     2521 gaaaagtatt gccttcaatt gcttggcaaa
//
LOCUS       DAOMAA010000214         2549 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_35716_length_2549_cov_2.969126, whole genome shotgun sequence.
ACCESSION   DAOMAA010000214 DAOMAA010000000
VERSION     DAOMAA010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35716_length_2549_cov_2.969126"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..496)
                     /locus_tag="PLO59_03780"
     CDS             complement(86..496)
                     /locus_tag="PLO59_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00253.1"
                     /translation="MKHLYFKQAAWAIVHAWAMGIVIIFMLSILSWDVVSTSVVPHAA
                     AFVITTIIAGVLMFFILSVAGWLYGQTKNDLSFNTMLHCLLPWFVCPFIILIIAVIFF
                     QSFEINVAIHVLQIMLFVQIALVRFIKQVTSIQQ"
     gene            complement(503..>2549)
                     /locus_tag="PLO59_03785"
     CDS             complement(503..>2549)
                     /locus_tag="PLO59_03785"
                     /inference="COORDINATES: protein motif:HMM:NF025150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HQV00254.1"
                     /translation="TILFLLNILLLTVNVLDVVYLWGGAGLPVGLNYTDFVHQGTGML
                     IASIVLAILLILYFFRGALHFYTDKKVLIILCCLWIVQNIFMIVSTAYRNHIYIEDYF
                     LTYKRLGVYVYLLLCVFGLITTAIKIIYKKSNWYLFRVNSWIVYIVLLTLSFYNWDAY
                     ITQYNIAAAEIKNRPIDKNYLADLSFNNLTQLLLMNDSIKQVTVAEDEHVTSSISFKR
                     KFYDSYFYERDFKSRLHFKLYKFLELQNNTQWPSYVFNYNHIAKQVDVLAKNKFSELH
                     LSNQGIETLKPIESLKNIQYLDVSHNPWKQEADLSSFKFLKNLNISYTQLDTLDSLPI
                     LNHLQTLNISGLENLQFQKLNRWKNVTSVDASKTNLTSIKQLPLWPKLTGLILAENEI
                     SDLNDLVRYKNLHMLDLSNALAQTQTLPVLPTLDTLNLSNNQGAMQNASLLDYLPFNN
                     LKNLNLSFNKIKHFPVSSNQLQQKASLCLSLLELNLSNNEMGSLTGLQSFSNLVSLNV
                     ADNKLTDISDIASLNHLKILNLNNNRYLKQLEPVTLLKTVTTLDIGGNKWLKDYQMIS
                     DMQQLKVLDMSDMNIDSIEFLNPLQQLTYLDLSYNHITHIEVLASKQNLQELILSGNI
                     IHDLTPLYKLKNLKRLVLINVAGIEQLRNLKQALPHTELITSMDDSALKTTAIEPLN"
BASE COUNT          842 a          416 c          437 g          854 t
ORIGIN      
        1 atacataaaa gtacgcttat ggttgatgag atggcaagca aagtaatggc actgctacgc
       61 atatcagtaa atcggacaat tttattcatt gttgtattga ggttacttgt ttaataaatc
      121 tgactaatgc aatctgaacg aatagcatta tttgaagtac atgtatagca acattaattt
      181 cgaagctttg aaaaaatata actgctataa ttaaaataat aaatggacat acaaaccatg
      241 gtaacagaca gtgtaacatg gtgttgaaac ttaaatcgtt tttggtttgg ccatataacc
      301 aaccggcaac gcttagtata aaaaacataa gtactccggc tattatggtt gttattacaa
      361 atgctgcagc atgcgggact acgcttgtgc taacaacatc ccatgacaat atgctaagca
      421 taaaaataat tactatgccc atagcccatg catgcactat ggcccatgca gcttgtttaa
      481 aatacaggtg tttcatttgt tactagttta gtggttcgat agctgttgtt tttaatgcgg
      541 agtcgtccat acttgtaatt aactcggtat gcggtaatgc ctgttttaaa tttcgtaatt
      601 gttcaatacc ggctacatta atcagtacca aacgttttaa attttttagc ttataaagtg
      661 gcgttaaatc atgaatgata ttgcctgaca aaataagctc ttgcaagttt tgctttgaag
      721 ctaacacttc gatatgggtg atatggttat agcttaaatc taaataggtg agttgttgca
      781 acggatttaa aaattctata ctatctatat tcatatccga catatctaac acctttaact
      841 gttgcatatc agaaatcatt tgataatcct ttagccactt gttaccgcca atatccagtg
      901 tagttactgt ttttaataat gtaacaggct caagttgttt taaatacctg ttgttattta
      961 aattgagaat ttttaaatga ttgagtgagg caatgtcact aatgtcggtt aatttattat
     1021 cagcaacatt taatgaaact aaatttgaaa atgactgtaa accggttaag ctgcccattt
     1081 cgttattact taaattaagt tcgagaaggc ttaaacagag tgatgctttt tgctgcagct
     1141 gatttgatga cactggaaag tgctttattt tattgaagct aagatttaag tttttaagat
     1201 tattaaaagg caaatagtct aacaagctgg cattttgcat agccccttga ttgttggata
     1261 aatttaatgt atctaatgtt ggcaatactg gcaaagtttg tgtttgcgcc aaggcattac
     1321 tcaagtctaa catgtgcaag tttttatatc gtaccagatc gtttaaatca ctaatctcat
     1381 tttcagctaa tatcaagcct gtaagtttgg gccaaagcgg caattgtttt atgctggtta
     1441 aattagtttt cgatgcatca acagaagtta catttttcca ccggtttaat ttttgaaact
     1501 gcaaattttc caatcccgaa atgtttaatg tttgcaagtg attgagtatg ggtaacgaat
     1561 ccaaagtatc aagttgtgta tagctgatat ttaaattttt taaaaatttg aacgaactta
     1621 aatcagcttc ttgtttccag ggattgtggc tcacatccaa atattgaatg tttttgagcg
     1681 attcgatggg tttcagggtt tcgatgccct gattgcttaa atgcaactcg ctaaatttat
     1741 ttttggccaa cacatctact tgctttgcaa tgtggttgta gttaaaaaca tacgatggcc
     1801 attgtgtatt gttttgcaac tctaaaaatt tatacaactt aaagtgcaaa cgcgatttaa
     1861 aatctctttc ataaaaatag ctgtcataaa acttgcgttt aaaacttatg ctgcttgtaa
     1921 catgctcatc ttctgcaacg gttacttgtt taatgctatc gttcataagc agcagttgcg
     1981 taaggttatt gaacgataaa tcggctaaat agtttttatc aataggccgg ttttttattt
     2041 cggcagcagc aatattgtat tgcgtgatgt atgcatccca gttgtaaaat gagagggtta
     2101 acaacactat ataaactatc catgaattaa cccgaaacaa ataccagttg ctttttttat
     2161 aaataatttt tattgctgtg gttattaagc caaatacaca aagcagcaga tacacatata
     2221 cacccaaccg tttataagta agaaaataat cttctatata tatgtgattt ctataggcag
     2281 tagaaacaat cataaaaatg ttttgtacaa tccacaaaca gcataaaata ataagcacct
     2341 ttttatctgt gtaaaaatgt aaggcccctc tgaaaaaata aagaattaaa agaatggcca
     2401 acacaatgga agcaattagc atcccggtac cctgatgcac aaaatcagta tagttaaggc
     2461 ccacaggtaa acctgctcct ccccataaat aaacaacatc taaaacattt acagtaagca
     2521 gtaaaatatt tagcagaaat aaaatggtt
//
LOCUS       DAOMAA010000215         2549 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_35717_length_2549_cov_2.913392, whole genome shotgun sequence.
ACCESSION   DAOMAA010000215 DAOMAA010000000
VERSION     DAOMAA010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35717_length_2549_cov_2.913392"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2549)
                     /locus_tag="PLO59_03790"
     CDS             complement(<1..>2549)
                     /locus_tag="PLO59_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00255.1"
                     /translation="SNSNNAATCALKCSLTNNGTIYCTNNLYINQSSTSFINSGSIDI
                     APGKFLKLTNITFTHNSGNITGNGTVEAEYSTLNLNAANIPGSRWSFIGCVLNSTQPT
                     LTNNKPLKFQSSNTINANVFNSDSIYIAGYNNNFVGTFTTSAASRILIDGSVTTAYVT
                     YTNGFTNNGIIELNSLYGYACDLYITNDTLVNAAGSTIRNAGIYAPSYSINGNVKNAG
                     TILLNHSMWFNGTSKTLLNTGTINILTSKILTVQNGTFTYNGGSMPGNGEMKFESAVA
                     NFNTATIPGKYMWFQYSTINSSQAVVNLNKAIRIGYFNTVNANVHLSDTIYISHYSNT
                     FSGNLTTTGAARLYFDALHSTGRLTFANGFTNNGQIIFNSSVGYGAQLTINNGTLTNA
                     AGSTIKATGIYNPVFYIYGNIVNNGTILCEQNLSIGSTGHTFTNNNIINIAAGKILDN
                     TANTFTYASGTTPGTGTFTFNSATANFNVANMPGKYFEFNNSILNSTFLPITNGRKIK
                     LKASNTINAAVFNTDTILISSYANRMNGALTTAGSSRIIYDCSIYTGALTVSKGFTNN
                     GKILFESTTGYTGYLTLDSNLIINAAGGEIRSDGLSGLNYIYAGVQNAGSINAIQNLH
                     IEKVDSSTNTGSINISANKYLKFYTGKLKYNSGSISGPGKLVFEYSEYEVNQNNIPTL
                     FTEFSASKLSSNLSLINNNAILQFYYNNTISANLKNKVTGTLKFSHYNNLVNGNFTNE
                     ANSLISIDGANYHSDVTFLNGFTNLGLIDLRSVTGYNSTLSVTNGELYNNNTGVIHAY
                     EPYGMRYLNAQLNNKSDLSVDRDLIINKPGADHKNVNGGDITIAANATLDI"
BASE COUNT          786 a          428 c          452 g          883 t
ORIGIN      
        1 tgtatgtcca aagtagcgtt agctgctatt gtaatatcgc caccatttac atttttatga
       61 tctgcaccgg gcttattaat aattaagtcg cggtcaacgc ttaaatcact cttattattt
      121 aattgcgcat taaggtaacg catgccataa ggttcataag catgtattac acctgtgttg
      181 ttattatata actcaccgtt ggttacactt aatgtactgt tatagcctgt tacactacgc
      241 aaatcaataa ggcctaaatt ggtaaaaccg tttaaaaatg ttacatcgct gtggtagtta
      301 gctccatcaa tagatattaa tgaattggct tcgtttgtaa agttgccatt tactaagttg
      361 ttataatgac tgaattttaa tgttcccgtt actttatttt ttaggttcgc acttatggta
      421 ttgttgtaat aaaactgaag tattgcattg ttgtttatca acgataaatt tgaacttaat
      481 ttagaagccg aaaattcagt aaataatgtg ggtatattat tttgattaac ttcatactca
      541 ctatattcaa atacaagttt ccccggaccc gatatgctac cagaattata tttgagtttg
      601 ccggtataaa atttcaaata tttatttgcc gaaatattta tcgaaccggt gttggtactc
      661 gagtccacct tttcgatatg caaattttgt atggcattaa tgctacccgc gttttgaaca
      721 ccggcatata tatagtttaa gccacttaaa ccatcgcttc gtatttcgcc tccggctgca
      781 tttataatta aattactatc caaagttaaa tagcctgtat agccagtagt actttcaaaa
      841 agtattttac cattattagt aaatcctttc gataccgtaa gtgctccggt atatatgctg
      901 caatcataaa ttatacgcga actgccggct gtagtaagtg cgccattcat acgattggca
      961 tacgatgata ttaaaatagt atcggtatta aaaactgcag catttatagt attagacgcc
     1021 tttaatttta tttttctgcc attggttata ggcaaaaaag tactgtttaa tatggagttg
     1081 ttaaactcaa aatatttacc cggcatattg gctacgttaa aatttgcagt agcactatta
     1141 aaagtaaaag ttcctgtgcc gggtgtggtg ccacttgcat acgtaaaggt attggcagtg
     1201 ttatctaaaa ttttgccagc agctatattt ataatattgt tattggtaaa agtatggccg
     1261 gtagatccaa tacttaaatt ttgttcgcat agtatggttc cgttattaac gatgttgcca
     1321 taaatgtaaa aaacgggatt ataaattccg gttgctttaa ttgtagaacc ggctgcattg
     1381 gttaaggttc cattgttgat ggttagttgc gcaccataac ctacactgga attaaaaatt
     1441 atctgaccat tgtttgtaaa accattggca aaggttaatc taccggtcga atgcagggca
     1501 tcaaaatata aacgcgcagc acctgttgta gttaaattac cactaaatgt attgctgtag
     1561 tgtgaaatat agatagtatc gcttaaatgt acattggcat taacggtatt gaagtaacca
     1621 atgcgtatgg ccttatttaa attaacaaca gcctgcgaac tgttaatggt cgaatattga
     1681 aaccacatat acttacccgg tatggtagct gtattaaagt ttgccacggc cgattcgaat
     1741 ttcatttcgc cattgccggg catactgccg ccattgtacg taaatgtgcc attttgaacc
     1801 gtaagtattt ttgaagttaa aatatttata gtacctgtat tgagtaatgt tttacttgta
     1861 ccattaaacc acattgaatg gttaagtaaa atggtacctg cattttttac atttccatta
     1921 attgaataag aaggtgcata aatacctgca ttgcgtatgg tactgccggc tgcattaact
     1981 aatgtatcat tggtaatata taagtcgcag gcatatccat ataatgagtt taattctatt
     2041 attccattat tggtaaatcc gttggtataa gtaacataag cagtagtaac agagccatca
     2101 attaaaatac gcgatgctgc cgatgttgta aatgttccaa caaaattgtt gttatagcct
     2161 gctatgtaaa tactatcact gttaaaaaca ttggcattaa tcgtattgct agattgaaac
     2221 ttaagtggct tattattggt tagagtaggc tgtgtactat ttaaaacaca accaataaac
     2281 gaccagcgac tacccggtat atttgctgca tttaaattta aggtactgta ctctgcctct
     2341 acggttccat taccggtaat attaccactg ttatgcgtaa aagtaatatt ggtaagtttt
     2401 aaaaatttac ccggtgctat atctatactg cctgaattta taaagcttgt gctgctttga
     2461 ttaatgtata aattattggt acaataaatt gtaccattat tggttaacga gcattttaaa
     2521 gcgcaagtag cagcattatt actgttact
//
LOCUS       DAOMAA010000216         2549 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_35721_length_2549_cov_2.734964, whole genome shotgun sequence.
ACCESSION   DAOMAA010000216 DAOMAA010000000
VERSION     DAOMAA010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35721_length_2549_cov_2.734964"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /locus_tag="PLO59_03795"
     CDS             <1..1009
                     /locus_tag="PLO59_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium transporter"
                     /protein_id="HQV00256.1"
                     /translation="IKQNAADHLHMIFSKTDSHYMDLPGLSVLIGGMLIVNLNYFGCN
                     QYITQRALGANLNTARNGLLFAAFLKLLMPIIVVLPGIAAYTLYKQGLFQNELMQDGV
                     LNPDKAYPTLLNLLPAGLKGLSFAALTAAIVASLAGKANSIATIFTLDIYKKYFNKNA
                     SETQLVQIGKITIIVALILAIIIAPFLGIDKKGGFQYIQEYTGFVSPGVFAMFLLGLF
                     WKKTTANAALFATIGGFIFSIVLKVLPAFINLSVLYSIGFAVPNAQGVFEIPFIDRMS
                     IVFVACIIGMYFISIYDAKRGVKTNGMQVALSEFKTTVTFTLLSITVILILVVLYTVF
                     W"
     gene            1074..1685
                     /locus_tag="PLO59_03800"
     CDS             1074..1685
                     /locus_tag="PLO59_03800"
                     /inference="COORDINATES: protein motif:HMM:NF019468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HQV00257.1"
                     /translation="MKPQLKIKLQTTDKFIECISWVALIFLWLIVIVSFKNLPNTIAT
                     HFNIAGTADNYGKKQMIFILPALATIIFAGLTILNNLPHLFNYPTTITNTNAYEQYTY
                     ATRLIRYLKLIVVVIFACIVFIIINHASGITVLGSWFLPLSIILIFTPIVYFIYKMIS
                     QKLQCIFYNCNCHFTFQLRTTSIEKRIFGLTINTVTNYLKKHL"
     gene            1682..2083
                     /locus_tag="PLO59_03805"
     CDS             1682..2083
                     /locus_tag="PLO59_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00258.1"
                     /translation="MKNAYQLITVLLITIFTTGCKKNDTRISDGTYAGTFTVTYSSGV
                     QSNNVVLEISGGKYNCSGNTNRIPAGGAGSFVLENNKLKFTDEQVWTADFDWNLILNG
                     SYNYTFDGQQLKITAHKNNVAYYKYDLLKIK"
     gene            2315..>2549
                     /locus_tag="PLO59_03810"
     CDS             2315..>2549
                     /locus_tag="PLO59_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQV00259.1"
                     /translation="MKPSIIYRQALTDDIAQLQRIRNSVTENVLTNPSLVTVNDYIKY
                     ITLEGKGWLCQVDSHIVGFAIVDLLGHNIWALFV"
BASE COUNT          807 a          453 c          425 g          864 t
ORIGIN      
        1 aattaaacaa aatgctgccg accacctgca catgattttt agcaaaaccg attcacatta
       61 catggatttg ccggggttat cggtacttat tggcggtatg ctaattgtaa acttaaacta
      121 ctttggctgt aaccagtata taacgcaacg cgctttgggc gcaaacttaa atacagcacg
      181 caacggatta ctttttgcag cgtttttaaa actacttatg cccattattg tggtgctgcc
      241 cggtatagct gcttacacac tttacaaaca aggattgttt caaaacgagc tgatgcagga
      301 tggtgtttta aatccagata aagcatatcc aacattactt aacttattac ctgctggctt
      361 aaaaggttta tcatttgctg cactcactgc ggcaattgtt gcatcgttag caggtaaagc
      421 caacagcatt gctaccattt ttacattaga tatttataaa aagtacttta ataaaaatgc
      481 cagcgaaacg caattggtgc agattggaaa aattaccatc atagttgcat tgattttggc
      541 cataattatt gctccgtttt taggcattga taaaaaaggc ggctttcaat acatacagga
      601 gtacacaggc tttgtaagcc ccggagtttt tgccatgttt ttgctcggtt tattttggaa
      661 aaaaactact gctaatgctg cattgtttgc aaccataggc ggctttattt tttcaattgt
      721 tttaaaagta ttgccggctt ttataaatct gtcggtatta tatagtattg gctttgctgt
      781 acccaatgcg caaggtgttt tcgaaattcc gtttattgac cgaatgagta ttgtgtttgt
      841 tgcttgcata attggcatgt actttattag tatctatgat gccaaacgtg gagtaaaaac
      901 caatggcatg caagtagcat taagcgaatt taaaaccaca gttacgttta cactattaag
      961 cattacagtt attctaattc ttgttgtttt atatacggtg ttttggtaat agaaaattta
     1021 tacagccatg ttgatatcgc catcattcaa gtattaattg ccttttataa aacatgaaac
     1081 ctcaacttaa aattaaactg caaacaaccg ataagtttat agaatgtata agctgggtag
     1141 cacttatttt tttatggcta atagtaattg taagttttaa aaacttgcca aataccatag
     1201 caacacattt taatattgcc ggcacggcag ataactatgg taaaaaacaa atgattttta
     1261 ttttaccggc tctggcaact atcatatttg cagggcttac catacttaat aatttaccac
     1321 atttatttaa ctaccccaca actataacca atactaatgc ttatgagcaa tacacgtatg
     1381 ccacacggtt aatacggtat ttaaaactta ttgtagttgt aatttttgct tgcattgttt
     1441 ttataattat taaccatgca tcaggtatta ctgttttagg ctcttggttt ttgcccttat
     1501 caataatact tatttttaca cccattgttt attttattta taaaatgatt agccaaaagt
     1561 tgcaatgcat attttacaat tgcaactgtc attttacttt tcaattacga actacaagca
     1621 ttgaaaagcg gatattcggc ctaactatta atacagtaac caactactta aaaaaacatt
     1681 tatgaaaaac gcttatcaac tcattaccgt tctgctgatt acaatattta caacgggctg
     1741 caaaaaaaac gacacaagaa ttagcgatgg tacttatgcc ggaaccttta cggtaactta
     1801 tagctcaggt gtgcaaagca ataatgttgt actcgaaata agtggtggta agtataattg
     1861 tagtggcaat accaaccgca tacctgctgg tggcgcaggc tcatttgtac ttgaaaacaa
     1921 taaattaaaa tttactgatg aacaggtttg gactgccgat ttcgactgga acttaatttt
     1981 aaatgggtcc tataactata cttttgacgg acaacaatta aaaattacgg cccataaaaa
     2041 taatgttgcc tattataaat atgaccttct aaaaattaaa tagtttttta tcatacagca
     2101 aaacgcatct gtctgttttt atcctttaat gtactccata ttttttgtgc ttcatttaag
     2161 ttgttattta aacatacttt ttaacacagc ttatatactt caagtatcaa ccgtttttga
     2221 agcgacttgt tgtaataatg taccctcaaa taaagtgcta taaaaaacag gctgctttgc
     2281 tttgaacctc tttttgtaat tctgaattaa tataatgaaa ccaagcatca tttaccggca
     2341 agcgctaact gatgatattg cacaattaca acgcatacga aacagtgtta ccgaaaatgt
     2401 gcttaccaac ccaagtcttg ttaccgtaaa cgattatata aaatatatta cgcttgaagg
     2461 caaaggctgg ctgtgtcagg tagatagcca cattgttggc tttgccattg ttgatttgct
     2521 gggccacaat atttgggcat tgtttgttg
//
LOCUS       DAOMAA010000217         2545 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_35825_length_2545_cov_2.744177, whole genome shotgun sequence.
ACCESSION   DAOMAA010000217 DAOMAA010000000
VERSION     DAOMAA010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35825_length_2545_cov_2.744177"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="PLO59_03815"
     CDS             <1..489
                     /locus_tag="PLO59_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HQV00260.1"
                     /translation="PYYLTYKAAELGYHAQVINSGRFVNDSVGFYIAKQTVKRMVAAG
                     KNNSEARVLVMGATFKEDVEDIRNSKVADVVNEFKSYGVSVDVVDPKANTDELMHEYG
                     FGLIPKPQPAYDAIVVAVSHNEYINLDEAYFKSIAAPRCILVDIKGLYRNKIKQLTYW
                     SL"
     gene            702..1400
                     /locus_tag="PLO59_03820"
     CDS             702..1400
                     /locus_tag="PLO59_03820"
                     /inference="COORDINATES: protein motif:HMM:NF025729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HQV00261.1"
                     /translation="MKKHFVLLVTILFLGIKTHAQFEGTCTITIESARTPLPIEMFMF
                     LKSDLMCMEIPSMQSSGFIKGIVNNTTQTLTTCIDKGGRKVAMRSNLNEMERFMPVSS
                     NAKPPVFTETTEKKKISGFNCTKVLMKSDDSEGTFWITQEWPIEMTQLITAISANKSP
                     GSAMLRGLSRLYTGRNGVSLETQVTNFKNKESYTVTITDVKPGKVDEKQFNIDDFQLM
                     DMPTMGGGQIKKTE"
     gene            complement(1470..>2545)
                     /locus_tag="PLO59_03825"
     CDS             complement(1470..>2545)
                     /locus_tag="PLO59_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00262.1"
                     /translation="ISPGLPKEKIASYLNSLQFKSASLTPLTWYNLDDGISVLSIPLY
                     NDDSCLLIDTPQAFIVNLNDSKPTQAQLKKLRGYMNEAVNKKIIVLSSYSPASIVNSF
                     RKNSEYVSIKDKNDYIVYINQLVSTLQADYFIPFASQVIFYRPDSAWANQYKVTYDDM
                     EKGWTAPKTKLLHPYSCIDLNTFDVTYVAPENYNHNSADIKEKVSNYLESVASVTLDE
                     ADEKALEKKLRHHRFILAPLFPRGIGFHIDNQDYYFNTWKGRLTRGKQNSSFALQVPA
                     QAMKDAVTYGHFGDLGITMFTLVILNRSNNPKLIYIFFILLTLHDYGHTVSFKNFYNW
                     IKSAFKNYNWNIPPMTAPKPVLV"
BASE COUNT          819 a          450 c          499 g          777 t
ORIGIN      
        1 ccttattacc tcacctacaa agcggccgaa ctaggttatc atgcccaggt aataaactcg
       61 gggcggtttg ttaacgatag tgttggtttt tacatagcca aacaaaccgt taagcgcatg
      121 gtagcagccg gaaaaaacaa cagcgaagcg cgtgtactgg ttatgggcgc cacctttaaa
      181 gaagatgttg aagatatacg aaacagcaaa gtagccgatg tggttaacga gtttaaatca
      241 tacggtgttt ctgttgatgt ggttgacccc aaagccaata ccgatgagct gatgcatgag
      301 tatgggttcg gattaatacc taagccccag cctgcctatg atgccattgt tgtagcggta
      361 tcacataatg aatatataaa tttagatgaa gcctacttca agtcaattgc tgctccacgc
      421 tgcattttag ttgatataaa gggtctgtat cgaaataaaa taaagcagct tacatattgg
      481 agtttgtaac ggtatcttta ttacattcga atcgtaagcc aattccaatt gaaggtgttt
      541 ttaaattaag tgtcgttttg tttattgcag acaagtgttt gctttttgta aattattgtt
      601 tcggtttgtt ttgtaataag tcaatgtaat ttcaaacatt cattccaaaa acaataattt
      661 taaagcataa agcactttat ttacgtgcat aaaattgcat catgaaaaaa cacttcgttt
      721 tattagtaac cattcttttt ttaggaatta aaacgcatgc acaatttgaa ggcacatgta
      781 ccataaccat tgaaagtgcg cgtacgcctt tgccaatcga aatgtttatg tttttaaaaa
      841 gtgatttaat gtgtatggaa attccgtcaa tgcaaagcag cggatttata aaaggtattg
      901 taaataatac tacacaaacc ttaactacat gcattgataa aggaggccgc aaagtagcta
      961 tgcgcagcaa tcttaatgag atggagcgtt ttatgcccgt atcatcaaat gccaaaccac
     1021 cggtttttac cgaaactaca gagaaaaaaa aaataagcgg gtttaattgt accaaagtgc
     1081 ttatgaaaag cgatgatagc gaaggtacat tttggataac gcaagagtgg cctatagaaa
     1141 tgacgcagct tataacagcc attagtgcaa ataaaagtcc gggcagtgct atgttgcgcg
     1201 gcttaagtcg tttatataca ggccgtaatg gggtgtcgtt agaaacgcag gtaaccaatt
     1261 ttaaaaacaa ggaaagctat acagtaacca ttaccgatgt aaaacccggc aaggtagatg
     1321 aaaagcaatt taatattgac gattttcaac taatggatat gcccactatg ggtggcggtc
     1381 aaatcaaaaa aaccgaatag taaaacatat atcagccgtc aattactgat ctgtttgacc
     1441 caaacaatta aatatttact tcttaaacat taaaccaaaa ccggtttggg cgcagtcatt
     1501 ggcggaatat tccaattgta atttttaaag gccgatttaa tccagttgta aaagttttta
     1561 aagcttacgg tatgcccata atcgtgtagt gtaagcaaaa tgaaaaatat ataaatcaac
     1621 ttaggattat tgctacggtt taaaatcacc aaagtaaaca tggtaatacc taaatcgcca
     1681 aagtgaccat aagtaactgc atctttcata gcttgtgcag gcacttgcag tgcaaaactc
     1741 gaattttgtt tgccacgggt taagcggcct ttccaggtgt taaaatagta atcctgattg
     1801 tcgatgtgaa acccaatgcc acgcggaaat aatggcgcta aaataaagcg gtgatgccgc
     1861 agtttttttt ccaatgcttt ttcatcagct tcgtccaaag taactgatgc aacagactct
     1921 aagtagttgc ttactttttc tttaatgtcg gcactgttat ggttgtaatt ttcaggggcc
     1981 acataagtta cgtcaaaagt atttaaatca atgcacgaat atggatgcaa cagcttggtt
     2041 ttaggggctg tccaaccttt ttccatatca tcatatgtaa ctttatattg attagcccat
     2101 gccgaatcgg ggcggtaaaa aattacctgg ctggcaaacg gaataaaata atcggcttgc
     2161 agggtgctaa ccaattgatt tatgtaaact atgtaatcgt ttttgtcctt aatactaacg
     2221 tattcactgt tttttctaaa gctgtttaca atacttgcag gcgagtagct gcttagtaca
     2281 attatttttt tgtttacggc ttcattcata tacccacgca gtttttttaa ttgtgcctga
     2341 gttggtttgc tatcattcag gtttacaata aaagcttgtg gcgtatcaat tagcaggcag
     2401 ctatcgtcat tatacaacgg aatacttagc acactaatgc catcatctaa attataccaa
     2461 gtaagcggag taagcgatgc cgatttaaat tgcaatgaat ttaaataaga cgctattttt
     2521 tctttaggta atccgggtga aatgt
//
LOCUS       DAOMAA010000218         2540 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_35960_length_2540_cov_3.463984, whole genome shotgun sequence.
ACCESSION   DAOMAA010000218 DAOMAA010000000
VERSION     DAOMAA010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35960_length_2540_cov_3.463984"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..144)
                     /locus_tag="PLO59_03830"
     CDS             complement(<1..144)
                     /locus_tag="PLO59_03830"
                     /inference="COORDINATES: protein motif:HMM:NF018299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HQV00263.1"
                     /translation="MKVYNNIDDFKPISNAIVTIGTFDGVHFGHQKIIERLKLLAAQV
                     DGQV"
     gene            223..1200
                     /locus_tag="PLO59_03835"
     CDS             223..1200
                     /locus_tag="PLO59_03835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HQV00264.1"
                     /translation="MVHQSNKQKIGYIILSLTIGVFIATLKFYGYKLTHANALLSDAL
                     ESFVNVVAAFFAFLSLIYASRPSDANHPYGHGKMEFFASGFEGCLILLAAFAMMYKSI
                     INQNNVVTQLDAGIAIVVITGFANGALGYFLKSKGKQLRSPTLKADGLHLISDAITSV
                     ALLAGLAAIYITKWYWIDLVITLLFSVYIGLMGIKLIRKAMAGLLDETDTETIATVVD
                     ALNKNRTAQWIDVHNLRAVQYGDALHIDTHLTLPYYLSLQQAHDAVKHFETSINQSLQ
                     VKVECFVHTDPCLPSSCSICTVDCKVRQKPFQKKIEWLPANVMHNAKHV"
     gene            complement(1202..2518)
                     /locus_tag="PLO59_03840"
     CDS             complement(1202..2518)
                     /locus_tag="PLO59_03840"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3887 domain-containing protein"
                     /protein_id="HQV00265.1"
                     /translation="MTFRKIIKLAALFVFVTLTTNAQPDSAKAIQTAQTFLDNLKAGQ
                     YLSCIKDFDWNLAGKVNTTALQNIWESLQKQQGKLLHTGAPYLVADSAYQTIYQLCSF
                     EKGKLDLKITTNSNYKIAGLFFVPPVSRLSYTLPDYAIADNVLETSLEIKNGNYTLPA
                     KLTYPKSGSKFPVVILVHGSGPNDMDETIGPNKIFKDIAYGLATKGVAVLRYTKRTKM
                     YAAQLDVNKLTLQQEVIDDAVAAVAVAKSVVAVDPQRIFICGHSLGGSCAPAIALKSN
                     NKAGIILMAAAARPLEDVALEQLKYLSDNSAEYLRKIEDFKVQASNVKLLDSTSDMES
                     FMLPFGLPQSYWLSVKNNKPAEEVKNYNGRILLLQGQRDYQVTLADFTLWQTALRNNS
                     KAQTRLYPTLNHLFMDGQGKSKPDEYNLPGHVSAQVIADIAQFCLQ"
BASE COUNT          746 a          481 c          492 g          821 t
ORIGIN      
        1 cacttgccca tctacctgtg cagccagtaa ttttaaacgc tcaattattt tttgatgccc
       61 aaaatgtaca ccgtcaaatg tgccaatggt aacaatggca tttgagatgg gcttaaagtc
      121 atcaatattg ttgtaaactt tcaatctatt tgttgatttg tatttgcaac aaagaagtgt
      181 ttttttgttt aaagattaaa acttccaaca ggattaggtg gtatggttca tcagagtaac
      241 aaacaaaaaa tagggtacat aatactatcg ctaacgatag gtgtttttat tgccacatta
      301 aaattttacg gatacaaact tacccatgcc aatgctttgt taagcgatgc actggaaagt
      361 tttgtaaatg ttgtggctgc attttttgca tttttaagtt taatttatgc atcgcgcccc
      421 agcgatgcaa atcacccata cggccatggt aaaatggaat tttttgcatc aggtttcgag
      481 ggatgtctta tcttattagc tgcctttgct atgatgtata agtcaataat aaatcaaaac
      541 aatgtggtta cacaacttga tgcaggtatt gccattgttg taataacagg ttttgctaat
      601 ggggcattag gttatttttt aaaaagcaaa ggcaagcaat tacggtcgcc cacacttaaa
      661 gccgatgggc ttcatttaat aagcgatgcc attaccagtg tagcactgtt agccggtttg
      721 gcagctattt acattaccaa gtggtattgg attgatttgg ttataacact tttattttcg
      781 gtttatattg gcttaatggg cataaaacta atacgcaaag ctatggcagg cctattggat
      841 gaaaccgata ccgaaaccat tgcaaccgtg gttgatgctt taaataaaaa ccgcacagcc
      901 caatggattg atgtacataa tttgcgtgct gtacaatatg gcgatgcatt gcacattgat
      961 acgcatttaa cgctgccgta ttatttaagt ttacaacagg cacacgatgc tgtaaaacat
     1021 ttcgaaactt ctattaatca atcgttacag gtaaaggttg agtgctttgt gcatactgac
     1081 ccttgcttac catcaagttg cagtatatgt acggttgatt gcaaggtgcg gcaaaaacct
     1141 tttcagaaaa aaattgaatg gttaccggcc aacgttatgc ataatgcaaa acatgtataa
     1201 tttattgcaa gcaaaactgc gctatgtcgg caataacctg tgccgataca tgaccgggta
     1261 aattatattc atcgggtttt gatttgccct gcccatccat aaacaaatga tttaatgtgg
     1321 gatataaacg tgtttgtgct ttactgttat tacgcaatgc cgtttgccat aatgtaaaat
     1381 cagcaagggt tacctgataa tctctttgac cttgcaacaa caaaatacgg ccattataat
     1441 tttttacttc ttcagcaggc ttattatttt taaccgatag ccaatacgat tgtggcaaac
     1501 caaaaggcaa cataaatgac tccatatcac tggtcgagtc taagagtttc acattacttg
     1561 cctgcacttt aaagtcttca atttttcgta aatattcggc actgttatcc gacaaatatt
     1621 ttaattgttc cagggctaca tcttcgagcg ggcgtgccgc tgcagccatc agaataatac
     1681 cggctttgtt attgcttttt aatgcaattg ccggtgcgca actgccacct aaactatgac
     1741 cgcaaataaa aatccgttgc ggatcaacgg caaccacgct tttagcaaca gccacagcag
     1801 ccacagcatc atctataact tcttgttgca gtgtaagctt gtttacgtct aattgcgctg
     1861 catacatttt ggttcgtttg gtatagcgca aaacggcaac accttttgtt gccaaaccat
     1921 aagcaatgtc tttaaaaatt ttatttgggc ctatggtttc atccatatca ttaggccccg
     1981 agccatgtac taaaattact accggaaact tacttccgct ttttggatag gtgagctttg
     2041 ccggcaaagt gtaattacca ttttttattt caagcgatgt ttcaagcaca ttatcggcaa
     2101 ttgcataatc aggcaatgta tagctgagcc gcgatacagg tggcacaaaa aacaatccgg
     2161 caattttata attgctattg gttgttattt ttaaatcgag tttacctttt tcgaaactgc
     2221 atagctggta aatggtttga tatgccgaat cggcaaccaa ataaggagct ccggtgtgta
     2281 atagtttgcc ttgctgtttt tgtaacgatt cccaaatgtt ttgcaatgca gttgtattaa
     2341 ccttgccggc caggttccaa tcaaaatctt taatacagga aaggtattga cctgctttta
     2401 aattatctaa aaaggtttgt gcggtttgta tggctttggc gctgtcgggc tgtgcatttg
     2461 tagtaagtgt tacaaaaaca aaaagtgcgg caagttttat tatttttcga aacgtcattt
     2521 gcttgaatta taatacgaat
//
LOCUS       DAOMAA010000219         2524 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_36385_length_2524_cov_2.644390, whole genome shotgun sequence.
ACCESSION   DAOMAA010000219 DAOMAA010000000
VERSION     DAOMAA010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36385_length_2524_cov_2.644390"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..827
                     /gene="mltG"
                     /locus_tag="PLO59_03845"
     CDS             <1..827
                     /gene="mltG"
                     /locus_tag="PLO59_03845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HQV00266.1"
                     /translation="DWLARFKKYDNKVKPGRYAFNSKQNNQDIINMLRSGNQSPVKLA
                     FNNIRTKQQLAGIVSQKIEADSTKLLQLLNDSAFISLQGFNRYNIMALFIPNTYEFYW
                     NTSADKFISRMHKEYDSFWNPMRMQQAANAGLKPVDVITLASIVQEETQMPADRPVIA
                     GVYINRIKKNMPLEADPTLKFALNNFAIKRLLNEDKKVESPYNTYKYAGLPPGPINLP
                     AIPYIDAVLNYTKHNYLFFCAREDLSGYSNFAQTYAQHLVNARKYQAELNRRNIKR"
     gene            827..1309
                     /locus_tag="PLO59_03850"
     CDS             827..1309
                     /locus_tag="PLO59_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00267.1"
                     /translation="MITEFEVMQAEDFFKQLEPAATQQYFEDFAKNQVNPFVYLMTLS
                     EDLSTDLIRHETLFLCGVIYQSFVIKYKQLQTATKTDLDDAQQQAITQIQAEALKQQN
                     SDAADQTIFQKNRQSFLLAYVMHEVARGRGVYTENDRLILFTILHTLILCFDKQVASA
                     "
     gene            1479..2234
                     /locus_tag="PLO59_03855"
     CDS             1479..2234
                     /locus_tag="PLO59_03855"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HQV00268.1"
                     /translation="MIYILIFFVAHWYLSLFSQTFFLHRYAAHKMFTMTPFWERFWYL
                     FLYITQGSSYLLPRGYAVMHRMHHAYSDTEKDPHSPHFFKEVFTMMMETKRIYYGIIS
                     GEFKAEEKFYKNYPVWDKLDKFGDSWFSRIGFGVLYTLFYIYFVPAGGWYWLLYLLLP
                     IHYLMGPVHGAIVNWSGHKYGYRNWDEDDHSRNSLFWDFLMMGELFQNNHHHYGARPN
                     FANKWWEFDPTYPIIRALDAIGIIKINLSSAKY"
BASE COUNT          826 a          462 c          441 g          795 t
ORIGIN      
        1 ttgattggct tgccaggttt aagaagtacg ataataaagt taaacccggc cgatatgcat
       61 tcaacagcaa acaaaacaac caggatatta taaatatgct gcgttccggt aatcaaagcc
      121 cggttaagtt ggcttttaat aatatccgta ccaaacaaca acttgctggt attgtttcac
      181 aaaaaataga agccgatagt actaagttgc ttcaactttt aaatgattcg gcttttattt
      241 cattgcaagg atttaaccgc tataacatta tggccctgtt tatacctaac acttatgaat
      301 tttactggaa tacaagtgcc gataaattta taagccgcat gcacaaagag tatgatagtt
      361 tttggaatcc catgcgaatg caacaagcgg caaatgccgg cttaaaacct gttgacgtta
      421 taactcttgc aagcatagta caagaagaaa cacaaatgcc tgccgacaga cctgttatag
      481 caggtgttta cattaatcgc atcaaaaaaa atatgccatt agaagccgat cctacactaa
      541 agtttgcatt aaataatttt gcaattaaac gattacttaa cgaggataaa aaagttgaat
      601 cgccttacaa tacttataaa tatgcgggtt taccgcccgg gcctattaac ttacctgcaa
      661 taccttatat tgatgccgta cttaattaca ccaaacacaa ttacctgttt ttttgcgcac
      721 gcgaagactt atcgggctac agcaattttg cgcaaaccta tgcccaacat ttagtcaatg
      781 cccgaaaata ccaagccgaa cttaaccgca gaaatattaa acgctaatga ttacagaatt
      841 tgaagtaatg caggccgaag acttttttaa acaattagag cctgctgcca cacaacaata
      901 ttttgaagat tttgccaaaa atcaggtgaa tccatttgtt tatttaatga ccttaagcga
      961 agatttatca actgacctta tcaggcatga aacgcttttt ttatgcgggg taatttacca
     1021 atcgtttgtt attaagtaca aacaactcca aaccgcaact aaaactgact tagacgatgc
     1081 gcagcaacaa gcaataactc aaatacaagc cgaagcatta aaacaacaaa acagcgatgc
     1141 ggctgatcaa accatttttc aaaaaaaccg gcaatcgttt ttactcgctt atgtaatgca
     1201 tgaagtagcg cgtggacggg gtgtatatac cgagaatgat agacttatac tttttaccat
     1261 cttgcataca cttattttgt gttttgataa acaagtagct tcagcgtaat ttttcaaaac
     1321 ccattagcaa ccgtttagtt aagatattga tatgatgtaa atcatgttat tacagccaaa
     1381 tgcttttgta catttgcgcc cgcaaaaagt tagttggcaa aatggtttta tggctaacaa
     1441 acaacactac aatacataat tattattaat caaaacacat gatttacatt cttatatttt
     1501 ttgtggcaca ttggtatcta tcgttgtttt cacaaacatt ttttttgcac cgttatgcag
     1561 cccataaaat gtttacaatg acaccttttt gggaaagatt ttggtacctg tttttataca
     1621 taacccaggg ttcgtcatac cttttaccgc gtggctatgc cgtaatgcac cgtatgcacc
     1681 atgcttacag cgatacagaa aaggacccac actcgcctca tttcttcaaa gaagtgttta
     1741 ctatgatgat ggaaaccaaa cgtatatatt acggcataat atctggtgag tttaaggccg
     1801 aagaaaaatt ttataaaaat tatccggttt gggataagct cgataagttt ggcgatagct
     1861 ggttttcgcg tattggtttt ggcgtacttt atactttatt ctacatttat tttgtacccg
     1921 caggtggatg gtattggttg ctttatttgt tacttccaat tcattaccta atgggtccgg
     1981 tgcatggagc aattgttaac tggagtggtc ataaatatgg ttaccgaaat tgggatgaag
     2041 acgaccactc gcgcaatagt ttgttttggg atttcttaat gatgggcgaa ttatttcaaa
     2101 acaatcacca tcattacggt gcacgaccca actttgccaa taaatggtgg gagttcgacc
     2161 caacctatcc aattattaga gcattggacg caataggcat aattaaaata aacttatcca
     2221 gtgccaagta ttaaacttta aagcctcctt aatacggagg ctttttttat gctgcttttt
     2281 aaaggcagta agtaatacga agtggtgttg atgtgcttta aactaatata cgttgttttt
     2341 tcattaaaca acaggaagtt ttgtgtttgc acatataaaa attaaaatat taaagcaacg
     2401 tttaatttat aaaattcaat tttgtgcatt ggccaaatta aaacttttgt ttgaatagtg
     2461 taagtaaaat gatgcatgtt gttaaataaa tattttgtta ttagaagtat tactatacgt
     2521 ttgc
//
LOCUS       DAOMAA010000220         2521 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_36453_length_2521_cov_3.275345, whole genome shotgun sequence.
ACCESSION   DAOMAA010000220 DAOMAA010000000
VERSION     DAOMAA010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36453_length_2521_cov_3.275345"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..1156)
                     /locus_tag="PLO59_03860"
     CDS             complement(29..1156)
                     /locus_tag="PLO59_03860"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HQV00269.1"
                     /translation="MQTIKLLIDMHGDQNRLFADFGYDYELIQILKTIPGARWSQTKK
                     QWHFKLNLEVLDLIKRKMIDKAYVDESQLNLQWTDLKTKQPTDKFASLDVAAQKALNY
                     YLMWMEQKRYSRQTIKNYWAHIVPFLYYYKSRNFMQLTVADVERYNYEVIIKNKLSVS
                     FQRSLIGSIKLFYAQVQNTKMDLRKLQRPFNEKRLPTVLSKEEVQSIIAATNNIKHKA
                     LLSLVYSCGMRISEVTNLKFKDLDKHRNLIRIEQAKGKKDRYVPYSSKLKQLLRNYYE
                     EWDPKPQVYIFEGQYGGKYSERSAGKVLQASLKTCNIIKDVSLHTLRHSYATHLLEAG
                     TDIRYIQELLGHNSPKTTMIYTQVSNKKLSEIKSPLDDLDV"
     gene            1474..>2521
                     /locus_tag="PLO59_03865"
     CDS             1474..>2521
                     /locus_tag="PLO59_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020603706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQV00270.1"
                     /translation="MLSVSNLSLRYGKRVLFEDVNLKFTPGNCYGVIGANGAGKSTFL
                     KMLSGEIEAATGQILMGPGERMSVLKQNHFEFDEFAVLQTVIMGNKPLWDVMLAKDLL
                     YNKPDFNEADGVKAGELETIFAEMEGWNAESDAASLLSGLGVDESFHQKLMKDLNGKE
                     KVRVLLAQALFGNPEILLLDEPTNDLDIETISWLENFLADFENTVIVVSHDRHFLDAV
                     CTHVADIDFAKITLYTGNYTFWYESSQLALRQKSDQNKKLEDRRRELQEFVERFSANA
                     SKSRQATSRKKLLDKLVIDDIQPSTRKYPGIIFRPERDCGDQILNVENLSAQASDGSV
                     LFNDVTFTVLKGDKV"
BASE COUNT          778 a          438 c          448 g          857 t
ORIGIN      
        1 tttatatttt gattggttca ggttaatttt aaacatctaa atcatctaat gggctcttta
       61 tttcacttag ctttttatta ctcacttgtg tatatatcat agtagtttta ggcgagttgt
      121 gtcccaacag ttcctgtatg taacgtatat cggttccggc ctcaagtaag tgcgtggcat
      181 aactatggcg caatgtatgc aaggaaacgt ctttaattat gttacaagtt tttaaacttg
      241 cctgtaaaac tttacctgca cttcgttcgc tgtatttacc accatactgt ccttcaaaaa
      301 tgtaaacctg aggcttagga tcccattctt cataatagtt tcttaaaagc tgctttagtt
      361 tgctactgta aggtacatat ctgtctttct tgccttttgc ctgttcaatg cgtattaagt
      421 tcctgtgctt gtctaaatct ttaaatttta agttagttac ttcgctaatt ctcataccgc
      481 aactataaac taaactaagc aatgctttat gtttaatatt gtttgttgca gcaattatac
      541 tttgcacttc ttccttacta agtacagtag gcaatctttt ttcgttaaac ggccgttgta
      601 gttttcgtaa atccattttg gtattttgta cctgagcata aaataatttt atcgagccta
      661 ttaaactacg ttgaaatgaa accgaaagct tgtttttaat tatcacttcg taattatagc
      721 gttcaacatc tgctactgtc aactgcataa aatttcgcga tttataatag taaagaaacg
      781 gaacaatgtg tgcccagtag ttttttatag tttgcctgct atatcgtttt tgttccatcc
      841 acattaaata ataatttaat gctttttggg ctgcaacatc taaactcgca aacttgtcag
      901 ttggttgttt ggtttttaaa tcagtccatt gcaaatttaa ctggctttca tcaacatagg
      961 ccttatcaat cattttacgc ttaatcaaat ctaacacctc taggttcaac ttaaaatgcc
     1021 actgtttttt agtttgactc catcgcgctc ccggtatcgt ttttagtatt tgaatcaatt
     1081 cataatcata tccaaagtca gcaaaaagcc ggttttgatc accatgcata tcaattaata
     1141 gcttaatagt ttgcatacca atatgtttaa gttcagtata tctcttaaaa gtggtttttg
     1201 tattttgaaa taagtacgtt acgagttact agcgaaaggg aatccaaatt ttaataaaaa
     1261 gttcaaaaaa tgaacatgac taaaaatgta atttataatg taactttcag ttgggtagca
     1321 acataagttt atcgccataa aggcaaacaa tttgtataaa acgcactttc gctattgtct
     1381 gattcaatct gcaacataac taatagttag catttgtatt taaacaaagg ccatattata
     1441 ttttacattt gcaggctaat ttaaaattgc tacatgttat cagtatcaaa tctatcgctc
     1501 cgctatggca aacgtgtttt atttgaagat gtaaacttaa aatttacacc gggtaattgc
     1561 tatggtgtta ttggagctaa tggtgccgga aaatcaacct ttttaaaaat gctttctggc
     1621 gaaatagaag cggctacagg ccaaatttta atgggtcccg gtgaacgaat gagtgttcta
     1681 aaacaaaatc atttcgagtt tgacgaattt gcagtacttc aaacagttat aatgggaaac
     1741 aaacctttat gggatgttat gcttgctaag gatttattat ataataagcc cgactttaac
     1801 gaagctgatg gtgttaaagc gggcgaactc gaaaccattt tcgcagaaat ggaaggctgg
     1861 aatgccgaaa gcgatgccgc ttcattacta agtggtttag gtgttgatga gtcgtttcat
     1921 caaaagttga tgaaagattt aaatggtaaa gaaaaggtaa gggtactctt agcacaagct
     1981 ttgttcggga atcccgaaat tttattgctc gatgagccca ccaatgactt agatattgaa
     2041 accattagtt ggctcgaaaa ctttttagcc gatttcgaaa acacagtcat agttgtttcg
     2101 cacgacaggc atttcttaga tgccgtatgt acacatgttg ccgatattga ttttgctaaa
     2161 attactttat atacaggcaa ctacactttt tggtatgaaa gtagtcagtt agccctacga
     2221 caaaaatccg atcaaaataa aaaattggaa gaccgcagac gcgagttaca agaatttgta
     2281 gaacgtttta gtgccaatgc atctaaatcg cgacaggcaa caagtcgtaa aaaattactt
     2341 gataaattgg tgatagacga tatacaaccc agcacacgca aataccccgg tattattttc
     2401 agaccggagc gcgactgtgg cgatcagatt ttaaatgtcg aaaatttaag tgcacaggct
     2461 tctgatggaa gtgtgttgtt taatgacgta acctttacag ttttaaaagg cgataaggtg
     2521 g
//
LOCUS       DAOMAA010000221         2519 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_36499_length_2519_cov_3.455763, whole genome shotgun sequence.
ACCESSION   DAOMAA010000221 DAOMAA010000000
VERSION     DAOMAA010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36499_length_2519_cov_3.455763"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="PLO59_03870"
     CDS             complement(<1..330)
                     /locus_tag="PLO59_03870"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HQV00271.1"
                     /translation="MKNFINHIRQFVNFTDDEAAAFYNVMTEVHFKKGAHLLEQGSIC
                     GQVYFITKGTVIYFMKQDDSEIINEVFFENCWVTEYGSFLAKSPATKSIRAMDEVTVL
                     ALSYNNMQ"
     gene            515..1273
                     /locus_tag="PLO59_03875"
     CDS             515..1273
                     /locus_tag="PLO59_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQV00272.1"
                     /translation="MQSIISVTNLHKQYNNYHAVKGISFEVYQGEIFGLLGSNGAGKT
                     TTLEIIETLRTKTSGTVLVDGLDVDNAADEIKKIIGVQLQAAGYYPNLNLSELLHLFA
                     GLYNHQIDTHAILKSVNLHDRAKAKYKELSGGQKQRFSIATTLINKPKIIFLDEPTTG
                     LDPQARRSLWDLIRDLRKQSVTVVLTTHYMDEAESLCDRVAIMEGGNIIALDTPQALI
                     DQLLASGFDPRREVKKANLEDVFLKIAGHSIDAV"
     gene            1299..2387
                     /locus_tag="PLO59_03880"
     CDS             1299..2387
                     /locus_tag="PLO59_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008509138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQV00273.1"
                     /translation="MKSYSQTKAMLAISKASLQSIMRSPSAVVFTLAFPLVFILVFGF
                     IGSGNIELVVAVMPQTQTNNLFYQTLETDSNVIKLHKNYSTQQIKKELEKGTLDAALA
                     IAENTDGTLQIQIQTSAASPDKGKLFGTIAGSIIDKINVSALQTKPAIQLSQTEVSGR
                     PYKRIDFILPGQLGFSILSTGVFGVAFVFFNLRQTLVIKRFFATPIKRIYIILGEALA
                     RIVFALMGAAFIILIGWAAFGFTLINGLLTFLTMLLLAALGLIVFMGFGFIVSGVAKN
                     ESTIPPFANMITLPQFLLSGTFFSISALPVWLQPICKLLPLTFLNDALRLVAFDGASL
                     IEVLPQITGLTIWGLLVYMGAARLFRWE"
BASE COUNT          784 a          466 c          472 g          797 t
ORIGIN      
        1 ttgcatgttg ttataactta aagctaatac agtaacttca tccattgccc ttatactttt
       61 tgtggctggc gattttgcca aaaaactacc gtactcagta acccagcagt tttcaaaaaa
      121 cacttcattt ataatttcac tgtcgtcctg tttcataaaa taaataaccg tgcctttggt
      181 aataaaatac acctgcccgc atatactgcc ttgctctaat aaatgagcgc cttttttaaa
      241 atgcacctca gtcattacat tataaaaagc tgctgcttca tcatctgtaa aatttacaaa
      301 ctgtctgata tgatttataa agtttttcat gtaacaaact tagatttttg taaatatcaa
      361 accgacatca aatcagtgaa atgaacataa tgtttgcagc aaattttttg tggcaaaagt
      421 cttatgtagt caattaccgt tacatttggc gccttatcag tttaaaaact ttttggctat
      481 tgtatagctg catctattat aacactaaaa gcaaatgcaa agcattatat cagttaccaa
      541 tttgcacaag caatacaaca attatcatgc agtaaaaggc atcagttttg aagtatatca
      601 aggcgaaatt tttggcttat taggatctaa tggtgccggt aaaactacaa cactcgaaat
      661 tattgaaacc ttgcgtacaa aaacatcggg tacggtttta gttgatggtt tagatgtaga
      721 taatgctgct gatgaaataa aaaaaataat tggtgtgcaa ctgcaggctg ctggttacta
      781 cccaaattta aacctaagcg aactacttca tttgtttgca ggtttataca atcatcaaat
      841 agatacacat gccatactta aaagtgtaaa cctgcatgat agagcgaaag caaaatataa
      901 agaattgagt ggcggacaga aacaacgatt ttcaatagca acaacactca taaacaaacc
      961 caaaattatt tttttagatg aacctactac cgggctcgac ccgcaggcgc gtagaagttt
     1021 atgggatttg attcgtgatt tgcgtaagca atcggtaacc gtagtgctta ccacacatta
     1081 catggatgaa gccgaatcgc tttgcgaccg tgttgccatt atggaaggtg gtaatattat
     1141 tgccttagat acaccacagg cattgataga tcaattatta gccagcggct ttgaccccag
     1201 acgtgaggtt aaaaaggcaa acctggaaga tgtgttttta aaaattgcag gccacagtat
     1261 tgatgctgtt taaatttaag ttgtaactgt taccatacat gaaatcatat agtcagacaa
     1321 aagcaatgct tgccattagt aaggcaagtt tacaaagtat tatgcgcagc cccagtgccg
     1381 ttgtatttac gttagccttt ccgttggttt ttattttggt ttttggtttt ataggcagcg
     1441 gaaatattga acttgtagtg gctgtcatgc cacaaaccca aaccaacaat ttattttatc
     1501 aaacattaga aacagatagc aatgtaatta agctgcataa aaattacagc acccaacaaa
     1561 ttaaaaaaga attagaaaag ggaaccttgg atgctgccct tgccattgct gaaaatacag
     1621 atggtacctt gcaaatacaa atacaaacca gtgcagcatc gcccgataaa ggcaaattat
     1681 ttggcaccat agccggcagt attattgata aaataaacgt tagcgcattg caaaccaagc
     1741 cggccataca acttagccaa accgaagtaa gtggcaggcc ctacaaacgc attgatttta
     1801 tattgccggg tcagttgggg ttttcaattt taagcaccgg tgtttttggt gttgcatttg
     1861 tgttttttaa cctgcgtcaa acattggtta ttaaacgttt ttttgctact cctataaaac
     1921 gcatttatat tattttgggc gaagcactgg cacgtattgt atttgcatta atgggtgccg
     1981 cttttattat tttaattggc tgggctgctt ttggttttac tttaattaat ggactgctta
     2041 cttttttaac catgctattg cttgcagcct tgggcttaat tgtttttatg ggctttggat
     2101 ttattgtttc aggtgttgca aaaaacgaaa gcacaattcc tccctttgct aacatgataa
     2161 cgctgccaca gtttttattg tcgggtactt ttttcagcat aagcgctttg cctgtttggt
     2221 tacaaccaat ttgcaaatta ttaccgttaa catttttaaa tgatgccctg cgtttagttg
     2281 catttgacgg ggcttcgctt attgaagtgc tgccacaaat taccggactt accatttggg
     2341 gcctgttggt ttatatgggt gcagcccgtt tattcaggtg ggagtagttt ttaattgata
     2401 caacaaattg atggtacaga taaaagaagc tgctgcaaac gatgccaact tactatgcca
     2461 gctgggcgca caaacatttg aacaagctta cggtgatgta aaagatgcaa acgacatat
//
LOCUS       DAOMAA010000222         2516 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_36573_length_2516_cov_3.022755, whole genome shotgun sequence.
ACCESSION   DAOMAA010000222 DAOMAA010000000
VERSION     DAOMAA010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36573_length_2516_cov_3.022755"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /locus_tag="PLO59_03885"
     CDS             <1..828
                     /locus_tag="PLO59_03885"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HQV00274.1"
                     /translation="LAVTWQKGTCKDVMSGETFDYIVTVTLTEKNNQNQIYKGCGKYL
                     ANENLNGNWFLEQVGNEFVNHSILQKSNPELIIDINGKSFTGHAGCNRFSGSLFVESK
                     TLRFTEISATEMACADGGFENRCMKALTATTTYKIVDNTLILSNPNGTTLTYKRNKFE
                     MKKSNALNKTWQLIELNGHEIIAGNQIKGLPQLTLNTQTLTYGGHTGCNNINGKFTNK
                     DNTISFLPGAMTRMACPGNLEDDFIKAFHKVNAYALSGNMLTLFVNDETVMVFKAMQ"
     gene            complement(915..2474)
                     /locus_tag="PLO59_03890"
     CDS             complement(915..2474)
                     /locus_tag="PLO59_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015809407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HQV00275.1"
                     /translation="MSTDKEIKTDSDIVVEKLYQTLIALNETPGQFPYTRGVQTDMYR
                     GKLWTMRQYAGFSTAEESNKRYKYLLQQGTTGLSVAFDLPTQIGYDSSHALADGEVGK
                     AGVAIDSLGDMEILFDGIALQNVTTSMTINATASTLLCMYIALAKKQGADLKLISGTI
                     QNDILKEYAARGTYIYPPKPSMRIITDIFEYCSKEVPKWNTISVSGYHIREAGANAVQ
                     ELAFTLADGKAYLQAAINKGLDIDVFAPRISFFFNAHNNLFEEVAKFRAARRMWANIT
                     KQLGAKNPKSQMLRFHTQTGGSTLTAQQPQNNIIRVTIQALAAVMGGTQSLHTNGFDE
                     ALSLPTEEAARIALRTQQIIGYESGVTQTVDPLGGSYYVEALTNAMEAKAYAYLQKID
                     EMGGAVAAIEAGYIQNEIAQSAYNYQKDIEHDTKIIVGQNKFVINEAPTKDLFTVDDS
                     IRQIQIEKLNAIKAKRNTLIVEAALEKIKNAATDGSNLMPHILYAVENYATLGEIADV
                     MRNVFGEYQGS"
BASE COUNT          767 a          493 c          464 g          792 t
ORIGIN      
        1 ttagctgtta cctggcaaaa aggtacttgc aaggatgtaa tgagtggcga aacgtttgac
       61 tatatagtaa ctgttacatt gacagaaaaa aacaaccaaa atcaaattta taaaggttgc
      121 ggtaaatact tagcaaacga aaacttaaac ggaaactggt ttttagagca agtgggtaat
      181 gaatttgtaa atcatagtat tttacaaaaa tcgaaccccg agttaattat tgatattaat
      241 ggaaaatcgt ttaccggaca tgccggttgt aatcgcttta gcgggagttt atttgttgaa
      301 tcgaaaacac ttcgtttcac cgaaataagt gctaccgaaa tggcatgtgc tgatggcgga
      361 tttgaaaaca gatgcatgaa agcattaacg gctacaacaa cgtataaaat agttgacaat
      421 actttgatac taagtaatcc aaacggtaca acgttaacat acaaacgcaa taaatttgaa
      481 atgaaaaaaa gcaatgcttt aaataaaaca tggcagttaa tcgaactaaa cggccatgaa
      541 attattgccg gtaatcaaat aaaagggtta cctcagctta cactcaatac acaaacacta
      601 acttatggtg ggcataccgg ttgcaacaat attaacggga agtttacaaa caaagacaat
      661 acaatttcgt ttttgcccgg agccatgaca cgcatggcat gcccaggcaa tttagaagat
      721 gattttataa aagcatttca taaagtaaat gcttatgcac tatcaggcaa catgcttaca
      781 ttatttgtaa acgatgaaac ggtaatggtt tttaaagcca tgcaataaga cttcaatttt
      841 agcagcaacc cataaccgaa catgtaagtt gtactttctt aaaaaatagt ttgcagctcg
      901 ttgtgcaaaa ctgtttaaga gccctggtac tcgccaaata cattgcgcat tacatcagca
      961 atttcgccaa gcgtagcata gttttcgaca gcatataaaa tatgtggcat taagttgctg
     1021 ccatccgttg ctgcgttttt tattttttct aaggctgctt caacaattaa tgtattacgt
     1081 tttgccttaa tcgcattcag cttttctatt tgtatctgcc ggatactgtc atccacggta
     1141 aataaatctt tagtgggtgc ttcattaatt acaaatttat tctgaccgac aataattttc
     1201 gtgtcatgct caatatcttt ttgataattg taggcacttt gtgcaatttc gttttgtatg
     1261 tacccggctt caatggcagc aactgcaccg cccatttcat caattttttg caaatacgca
     1321 taggcctttg cttccatggc attggttaat gcttctacat aataggaccc gcctaacgga
     1381 tcaactgttt gcgtaacacc gctttcgtaa cctataattt gctgcgtacg taatgcaatg
     1441 cgtgcagcct cttcagttgg taatgaaagt gcttcatcaa aaccatttgt atgtaacgat
     1501 tgtgtgccgc ccattactgc tgctaaggcc tgtatggtaa ctctgattat attgttttgt
     1561 ggttgttgtg ctgttagtgt tgagccacct gtttgtgtat gaaagcgcaa catttgcgat
     1621 ttgggatttt tcgcacccaa ttgtttggta atgtttgccc acattctgcg tgctgcccta
     1681 aacttggcaa cttcttcaaa caaattatta tgtgcattga aaaaaaatga aatgcgtggt
     1741 gcaaacacat caatatccaa acctttgttt atggcagctt gtaaataagc tttgccatcg
     1801 gctaaagtaa aagccagttc ctgcactgca ttagcaccgg cttctctgat atgatagccc
     1861 gatacactaa tcgtattcca cttaggtact tctttactgc aatactcaaa tatatcggtt
     1921 atgatacgca tacttggttt aggcggatat atataagtac cacgtgctgc atactctttt
     1981 aaaatatcgt tttgtatcgt gcccgaaatt aatttcaaat cggcaccttg cttttttgcc
     2041 aaagcaatat acatgcacaa taatgtactg gcagtagcat taatggtcat actggtagtt
     2101 acattttgca atgcaatgcc atcaaataaa atttccatat cacctaacga atcaatagct
     2161 acacctgctt tgccaacttc gccatcggcc aatgcgtggc tactgtcgta gccaatttga
     2221 gtaggcaaat caaaggcaac acttaagccc gtggtacctt gctgaagtaa atatttgtat
     2281 cgtttattcg attcttcggc agtactaaac ccggcatatt ggcgcatagt ccaaagtttg
     2341 ccacggtaca tatcggtttg cacgccacgt gtgtagggaa actgccccgg tgtttcgttt
     2401 aatgctataa gcgtttgata caatttttca actactatgt cactatcggt tttaatttcc
     2461 ttatcggtgc tcatgccaat taaatctata ctaaataaat gattattgat atttgt
//
LOCUS       DAOMAA010000223         2513 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_36658_length_2513_cov_2.905207, whole genome shotgun sequence.
ACCESSION   DAOMAA010000223 DAOMAA010000000
VERSION     DAOMAA010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36658_length_2513_cov_2.905207"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /locus_tag="PLO59_03895"
     CDS             <1..884
                     /locus_tag="PLO59_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00276.1"
                     /translation="GNINRDRATEFTQAAYYNSSYQIATQYGYVSAQAGLQFKTDSLF
                     IKRNKNFERYVTVDSINGLDTIKIKTDVLKGKSGIEKKKLKAEKFLRFNAGFTGALGQ
                     TVTETSDVFVRTYKRNNVGVILQRDTILYATNAETKRDLPGAIGVGFSLQEINKWLFG
                     IDAGYQNWSVYNERNNALDDAYKVIVGGEWIPKYNSAKYAQRIAYRAGLRYEKTNLSL
                     NNTAIHDYTLSIGAGLPIRKDGVTSAFSAYHSKLNVALEFGIKGTTKNSLLQENYVRL
                     TIGVNFADQWFIKRKYD"
     gene            963..1595
                     /locus_tag="PLO59_03900"
     CDS             963..1595
                     /locus_tag="PLO59_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQV00277.1"
                     /translation="MKPIKIALVEDRDDIRNAMQILLNSEEGFKCTHVFTNGADAVNQ
                     IPKLEIDVVLMDIDLPVLNGIESVKQLKTIKPDLLFMMVTVFEDDEKIFDALAAGASG
                     YILKKTAPARILESITELLAGGSPMNAQIAQRLVKHFQPQIQLNETKEQILSKREIEI
                     IEQLAKGLLYKEIAFILNISIGTVKQHLHRIYEKLHVQNKTEAINKFYRH"
     gene            complement(1618..>2513)
                     /locus_tag="PLO59_03905"
     CDS             complement(1618..>2513)
                     /locus_tag="PLO59_03905"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV00278.1"
                     /translation="GQTTQQINTFYAGTYSVTVTNWFGVSATSAPVNVVQNVKPKSLI
                     TSSGLMSICNGNSITLTVSESKTYLWSNGQTTRSIVVMQPGIYYVTVSNNKGCTSVST
                     PAVVTLGNCNVSCPAPYNLATTNITSNVARLNWDENYVADSLIYTLTNTATNASINYT
                     VSGILDFTTVFNLVPNTLYKWSLKGKCGSVISASSDTTLFTTWPVYGNSRLAMYQQQI
                     KLYPNPILKSIKLQILFEEAETVQLIVCDALGKVVYTTMHNLPSGVSVLQIPFEEYAA
                     GFYNLQLIGNSITENIKFQKL"
BASE COUNT          887 a          416 c          449 g          761 t
ORIGIN      
        1 ttggaaatat taatcgtgat agagctactg agtttacgca agcagcatac tacaacagca
       61 gttatcaaat tgctacacaa tatggttatg ttagtgcaca agcaggctta caattcaaaa
      121 ccgattcgct ttttataaaa cgaaataaaa atttcgaacg atatgtaaca gttgattcta
      181 ttaatggctt agatacgatt aaaataaaaa cagatgtatt aaaaggcaaa agcggaattg
      241 aaaaaaagaa attaaaagca gaaaagtttc tccggtttaa tgcagggttc accggtgctt
      301 taggccaaac cgttaccgaa accagcgatg tatttgtaag aacttataaa cgcaacaatg
      361 taggtgtaat attacagcgc gatacaatat tgtatgcaac caatgcagaa acaaaacgtg
      421 atttacccgg tgccattggg gttggatttt cactacaaga aataaataaa tggttatttg
      481 gaatagatgc aggttaccaa aactggagtg tgtataacga aagaaacaac gctttagatg
      541 atgcttataa agtaatagtt gggggcgaat ggatacccaa atataattcg gctaaatatg
      601 cacaacgtat tgcatatcgt gccggcttac gctatgaaaa aacaaattta agtttgaaca
      661 atacagccat acatgactat actttaagca ttggtgcagg cttaccaatt agaaaagatg
      721 gtgttacatc ggcatttagt gcataccatt cgaaactcaa tgtagcatta gagtttggaa
      781 ttaaaggaac tacaaaaaat agcttacttc aggaaaatta tgtacgctta accattggtg
      841 taaattttgc cgaccaatgg tttattaaaa gaaagtacga ctaataaata taacaaaagc
      901 tatattttta ttttcgagta taaaggtgtt tttagcattc taacaaataa aatccctttg
      961 cagtgaaacc cattaaaatt gctttggttg aagatagaga cgatatccgt aacgctatgc
     1021 aaatattgct taatagcgag gaaggtttta agtgtacaca tgtatttact aatggtgccg
     1081 atgcagtaaa tcaaatccca aaactcgaaa ttgatgtggt gttaatggat atagatttac
     1141 ctgtacttaa tggtattgaa agtgttaagc aacttaaaac aataaaacca gatttattat
     1201 ttatgatggt aaccgttttt gaggatgatg aaaaaatttt tgatgcacta gctgcaggtg
     1261 catccggtta tattctaaaa aaaacagctc cggcacgcat acttgagtca attacagaat
     1321 tgcttgctgg tggttcgcca atgaatgcac aaattgccca aagactggtt aagcattttc
     1381 aacctcaaat acagttaaat gaaactaaag aacaaatttt aagtaaacgt gaaattgaaa
     1441 ttattgagca gctcgctaaa ggtttgctct acaaggaaat tgcattcatt ttaaatataa
     1501 gcataggtac cgtaaagcaa catttacacc gtatttatga aaagcttcat gtccaaaata
     1561 agaccgaagc aataaataaa ttctataggc attaaaatct tttaagtttt aaactaccta
     1621 aagcttttga aacttaatat tttctgtaat gctattgcct attaattgta aattataaaa
     1681 acctgctgca tattcctcaa aaggaatttg caacacacta acaccacttg gcaaattatg
     1741 catggttgta taaacaactt ttcctaatgc atcacaaaca attaattgaa ctgtttccgc
     1801 ctcttcaaac aatatttgta gtttaataga tttcaaaatc ggatttgggt aaagcttaat
     1861 ttgctgctga tacatagcca agcgtgaatt accataaact ggccaggttg taaataatgt
     1921 tgtatcgctt gatgctgaaa ttacgctacc gcacttgcct tttaaactcc acttatacag
     1981 tgtgtttggt actaaattaa atactgtggt aaagtcaagt attccgctta ccgtataatt
     2041 aatagaagcg ttggttgctg tattagttaa agtgtaaatt aagctatcgg ctacataatt
     2101 ttcgtcccaa tttaatcggg caacatttga tgtaatattt gttgtagcca aattatatgg
     2161 agcgggacat gaaacattgc aattacccaa agtaacaaca gcaggtgtgc taacactggt
     2221 gcaacctttg ttattcgaca ccgttacata ataaataccc ggctgcatca ctacaatgct
     2281 tctggtcgtt tgaccattac tccacaaata ggttttcgat tcgcttacgg ttaaagttat
     2341 ggagttgcca ttacaaattg acattaagcc cgaagaagtt attaacgatt tgggcttaac
     2401 attttgaacc acattaacag gtgcagaagt tgcacttacg ccaaaccaat tagttacagt
     2461 tacactatat gtacctgcat aaaaagtatt gatttgttga gtagtttgcc ccg
//
LOCUS       DAOMAA010000224         2509 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_36772_length_2509_cov_3.128769, whole genome shotgun sequence.
ACCESSION   DAOMAA010000224 DAOMAA010000000
VERSION     DAOMAA010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36772_length_2509_cov_3.128769"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..161
                     /locus_tag="PLO59_03910"
     CDS             <1..161
                     /locus_tag="PLO59_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013997600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HQV00279.1"
                     /translation="ISDSTTSYGSYSGAVPNEKITWGKLDITTPKFIVESDATIVAPL
                     IFAWILGQ"
     gene            178..1290
                     /locus_tag="PLO59_03915"
     CDS             178..1290
                     /locus_tag="PLO59_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00280.1"
                     /translation="MLFLSFGDAPSGVLSSQVFDVVKYCRTLTKQRIICLAFISVRGY
                     FKNRVIIKNQINDCWVVPMYPTIHLWRFNLLTLAVICLLTKQRNIIARNAMAVNLALA
                     VKKTGLIKKIALDGRGAIAAEWNEYDVMPNKKLKSEINEIEKTAVLQSDFRLAVSNPL
                     VHYWHNRYGYNSKQHVIIPCTISFEKTFNNIDIAWPYHPHEIVLVYAGSVAGWQSFDL
                     LKLFLNDVLTQTVNHQVLFLSKTNTSIETLKQSFPNQVKHMWLPHEQVLQILERCDYG
                     ILIREQTVTNEVASPTKFAEYLSAGLDVLISEKLGDYTAFVQQNNCGIVWEPGSKTIL
                     LKSSVAQKNHNKILAQQHFSKEAFKSDYMRLLEAMA"
     gene            1598..>2509
                     /locus_tag="PLO59_03920"
     CDS             1598..>2509
                     /locus_tag="PLO59_03920"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQV00281.1"
                     /translation="MKRIRTTCLFVFFAVYIWAQPSTKSIDSLKQVVSTTNVETVKYN
                     SWLQLSQLYLQFDTSLSLTYLHIGLDNARLQKNILYETEFLSQLGTLHFTFENNDSAL
                     YYLTACTSKFDNGTPPRLMFECYIQLGNAYWKNGDFQKSMQALLKAENYANPPDEALK
                     RSQLYYTMGGVMMDLQQPEKALEYCKKCITVLEQNNLTQHLSKNYSSCGALFFMLGKP
                     AEALALFNKGLALAIKFSDTKSYTQATMNLGNYYNEMDNLDSALYFYNLSRIAHLKST
                     HTDQQYASLLLNIASVLDQQKKYNEAEL"
BASE COUNT          806 a          463 c          449 g          791 t
ORIGIN      
        1 agattagtga tagcacaacc agctatggtt cgtacagtgg tgccgtacca aatgaaaaaa
       61 ttacatgggg taagttagat attaccacac ccaagtttat tgtagaaagc gatgcaacta
      121 ttgtggcacc gctaatattt gcctggattt taggacaata aacatccgtc agcctttatg
      181 ttgtttctat cctttggcga tgcaccttcg ggcgtattaa gcagtcaggt atttgatgtg
      241 gttaagtact gccgtacatt aaccaagcaa cgtataatat gccttgcatt tatttctgta
      301 cgcggctact ttaaaaacag agttatcatt aaaaatcaaa ttaatgattg ttgggtggta
      361 cctatgtatc caaccataca cctatggcgc ttcaatttat taacactggc tgttatttgt
      421 ttactaacca agcaacgaaa cattattgcc cgaaatgcaa tggcagttaa tttggcctta
      481 gcggtaaaaa aaacaggcct tattaaaaaa attgcgcttg atgggcgtgg tgcaattgct
      541 gccgagtgga atgaatatga tgtaatgcct aataaaaaat taaaatccga aattaatgag
      601 attgaaaaaa cagcggtgct tcagtccgat tttcgtttag ctgtttcaaa tcctttagtt
      661 cattactggc ataaccgtta tggctataat agcaaacaac atgttattat tccttgcacc
      721 attagtttcg aaaaaacatt taacaacatt gacattgcct ggccctatca cccgcatgaa
      781 attgtattag tttatgcagg gtcggttgca ggttggcaat cgtttgattt gctgaagctt
      841 tttttaaacg atgttttaac tcagacggtt aaccaccagg ttttgttttt aagcaaaaca
      901 aatacatcca tcgaaacatt aaagcaatca tttcccaatc aggtaaaaca catgtggtta
      961 ccacatgaac aagtattgca aatattagaa cgctgcgact atggcatctt aatcagagaa
     1021 caaaccgtta ccaacgaagt ggcatcgccc actaaatttg ccgaatattt atcggccggg
     1081 ctggatgtac ttatcagtga aaaattaggc gattacactg cctttgttca acaaaataat
     1141 tgtggcatag tttgggagcc tggcagtaaa accattttat taaaatcaag cgttgcgcaa
     1201 aaaaaccata acaaaatact ggcacaacaa cattttagca aagaggcttt taagtcggat
     1261 tacatgcgtt tattagaagc aatggcttaa gctttaatcg tatttaattt tgtggatttt
     1321 gctgacatca atagttgttt tgtgtttttt ttgttacgct acagcttgct ttctaatttt
     1381 tcactatttc aaacaacaac agtttatttg ggtaatacag cagaagtaca tttttcaatt
     1441 ccaaaatgct gttttaatgc cagcaaatcg ctatttcgtt tttcgttgtt ggcattgctt
     1501 tttcagctta caattaatta tttgtacatg taaacgggca tgcatgcagc gtgctgcatt
     1561 taaaaaccat atttttcaca cattaaaaca ataggctttg aaacgtattc gaacaacatg
     1621 tctgtttgtg ttttttgcag tttatatatg ggcacaacca agtacaaaat ccatcgacag
     1681 tttgaaacaa gttgtttcaa caactaatgt tgaaacggta aaatacaatt cctggttaca
     1741 actatcccaa ctttatctgc agtttgatac ttcgctgtcg ttaacctatt tgcatatagg
     1801 tttagacaat gcacgtttac aaaaaaacat tttgtacgaa accgagtttt tatcgcaact
     1861 cggcaccctg cattttacat ttgaaaacaa tgacagtgcc ctgtattatt taactgcatg
     1921 cactagcaaa tttgataatg gtacaccgcc tcgtttaatg tttgaatgtt acatacagtt
     1981 aggcaatgct tattggaaaa atggcgattt tcagaaaagt atgcaagcat tactaaaagc
     2041 tgaaaactat gcaaaccctc ctgatgaagc attgaaacgt tcgcagcttt attatactat
     2101 gggtggtgta atgatggatt tacagcagcc agaaaaagct ttggagtatt gcaaaaagtg
     2161 cattaccgta ttggaacaaa ataatttaac ccagcatctt tcaaaaaatt acagctcttg
     2221 tggtgcttta ttttttatgc tgggcaaacc tgctgaagcg ttggcgcttt ttaataaagg
     2281 tttagccctg gcaataaaat tttctgacac caaatcgtac acacaagcta ccatgaattt
     2341 aggaaactac tataatgaaa tggataactt agatagtgca ctttacttct ataacctatc
     2401 tcgtattgcg catttaaaaa gcacacacac cgaccagcag tatgcatcat tattattaaa
     2461 tatagccagt gttttagacc aacaaaaaaa atataacgaa gccgagctg
//
LOCUS       DAOMAA010000225         2509 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_36779_length_2509_cov_2.482885, whole genome shotgun sequence.
ACCESSION   DAOMAA010000225 DAOMAA010000000
VERSION     DAOMAA010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36779_length_2509_cov_2.482885"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1983)
                     /locus_tag="PLO59_03925"
     CDS             complement(<1..1983)
                     /locus_tag="PLO59_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00282.1"
                     /translation="MPKPLRFCFVFLCAWLLPCMLNGQMNSRNGKTLPCSGTIRILIL
                     FIEYDYDINDSLDPAKPYEPFSKWRKGQLPIWKDSLFDAYETADFKGKLTQFYSESSF
                     NNFIVLGDYYPKLITIKESEVKNINEGGFNIKTIVAKTQKETGFKSNAGLGINEFDNW
                     TLTQPGAKKITPSTDSIPKWDHVMLLYRNARNLPNSNGRAVPATLGKLFGYDCDTYSN
                     FGTLSDLPFGICRHEFTHLFLGDNNFHSGGGERNGQGSYWIGVQCMWSVIGAAHASLA
                     CCNAWDRNRLGWKPSDKKFYTGCLDVNGHEVASVLADTSGTFTLRDFVTTGDAIKLKL
                     PYLQPYEFEQFLWIENHQGSKRNGTTFDHFVFENETCVQPATPGLYMYIQADRNNYNT
                     GNIYNGSADYLHPLPADGLFDIQYDSVLQKNNCVNDVLMRPFSKPLALQNPLTGNHSL
                     EIPTRDFNNNQIIEYAELGILAIEKKDNQFIKNLSYLGNSAVAFTATGNNKIGIGTNP
                     STTNLITAVSNASAPSFKQNYKEPIFNCRTSYLNGWSVKILNQLPNGNITVQIKHNDF
                     VIGNNMRWCADSIIVPIACDEAQPLTIKNKSTLIIDRGLTPTRVSKPLRFNNNKVFTD
                     TSQLILPPNTNMVLDTGSTLIIDNGSKLHLLKGSKIY"
     gene            complement(1967..>2509)
                     /locus_tag="PLO59_03930"
     CDS             complement(1967..>2509)
                     /locus_tag="PLO59_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HQV00283.1"
                     /translation="KNIVEVAERLQDIGIAALTIHGRTRSQMYKGPADWTLIGAVKNN
                     PRMHIPIFGNGDADSPQKVLDLKNTYGIDGVMIGRAAIGYPWIFNEVKHYLKTGLLLP
                     TPTIEQRVATCKMHFDKSVEWKTERGGILEMRRHYTNYFKGLNHFKDLRLQLVTAETV
                     EHIHTLFNEIIQHYCHAETT"
BASE COUNT          682 a          492 c          515 g          820 t
ORIGIN      
        1 atatatttta ctgcctttca ataaatgcaa tttgcttcca ttatcaatta ttaaggtgct
       61 gccggtatca agcaccatat ttgtgttggg tggtaaaatc aattgcgagg tatcggtaaa
      121 caccttattg ttattaaagc gaagtggttt cgaaactcgt gtaggtgtta gtcctctgtc
      181 aataattaac gtgcttttat ttttgattgt taatggttgt gcttcatcgc aggcaatagg
      241 tacaatgata ctatcggcac accaacgcat gttattgcct atcacaaaat cattatgttt
      301 tatttgaacg gttatattgc cattgggcaa ttggtttagg atttttaccg accatccgtt
      361 taggtatgac gtacgacaat tgaatatagg ttctttatag ttttgtttaa atgatggagc
      421 agaggcattg ctaacagccg taattaaatt ggtggtgctc gggttggtgc cgataccaat
      481 tttattattg cctgtagctg taaacgctac ggcactgttg cctaagtagc ttaagttttt
      541 aataaattga ttgtcttttt tttcgatagc taaaataccc agttcggcat attcaataat
      601 ttgattgtta ttaaaatcgc gtgtaggtat ttctaaacta tggttgccgg ttaatggatt
      661 ctgcaatgct aaaggtttgc taaaaggtcg cattaataca tcgtttacgc agttgttttt
      721 ttgcagaacc gagtcgtatt gtatgtcaaa caaaccatcg gccggtagcg ggtgtaagta
      781 atcggcactg ccattataaa tgttgcctgt gttataattg ttgcggtcgg cctgtatgta
      841 catgtataaa cccggtgttg caggttgtac acaggtttcg ttttcaaaaa caaaatggtc
      901 aaatgtagta ccattgcgtt tactaccctg gtgattttct atccataaaa actgttcaaa
      961 ttcatacggt tgtaaataag gtagttttaa tttgatggca tcgcctgtgg taacaaaatc
     1021 gcgtaaggta aaagtgcccg aagtatcggc taataccgat gccacttcat ggccatttac
     1081 atccaagcaa ccggtgtaaa attttttatc cgatggtttc caacccaacc tgtttctgtc
     1141 ccaggcattg caacaagcca gcgaagcatg tgctgcacca ataacactcc acatacattg
     1201 tacgcctatc caataactcc cttgcccgtt gcgctcgcct ccgcccgaat gaaagttatt
     1261 atcgcccaga aacaaatgtg taaactcatg cctgcatata ccaaagggca aatcgctaag
     1321 ggtgccaaag ttcgaatagg tatcacaatc gtaaccaaac aatttgccta aggttgccgg
     1381 tacagcgcgg ccattgctgt tgggcaaatt acgtgcattg cgatacagca gcattacatg
     1441 atcccattta ggtatgctgt cggtactggg tgtaattttt tttgcccccg gttgtgttaa
     1501 tgtccaatta tcaaattcgt taatgcccaa tccggcattg cttttaaatc cggtttcttt
     1561 ttgtgttttt gcaacgatgg tttttatatt aaagccgccc tcatttatat tttttacttc
     1621 gctttcttta atcgtaatca gtttgggata gtaatcgccc aggacaataa aattattaaa
     1681 cgatgactcg ctgtaaaatt gagttagctt gcctttaaaa tcggctgttt cgtaggcatc
     1741 aaacaaactg tctttccaaa tgggcagttg ccctttgcgc catttactaa agggttcata
     1801 aggtttggca gggtctaatg agtcgttaat gtcgtaatcg tactcaataa ataaaatcag
     1861 aatcctgatg gtaccactgc acggcaaagt tttaccattg cggctattca tttggccgtt
     1921 aagcatacat ggcaacagcc atgcacataa aaaaacaaaa caaaacctaa gtggtttcgg
     1981 catggcagta gtgttgtatt atttcattaa acaaggtatg gatgtgttcc acagtttcgg
     2041 cagtaactaa ttgaagtctg agatctttaa aatgatttaa gcctttaaag tagttggtgt
     2101 aatgcctgcg catttctaaa atgcctccgc gttcggtttt ccattcaacc gatttgtcaa
     2161 aatgcatttt gcaagtggcc actctttgct cgatagtggg tgttggtagt agcaaccctg
     2221 tttttaaata atgttttact tcattaaata tccacgggta gccaattgct gcacggccaa
     2281 tcattacgcc atcaataccg taggtgtttt ttaaatccaa aactttttgt ggcgagtcgg
     2341 catcaccatt accaaaaatg ggtatatgca tacgcgggtt atttttaaca gcaccaatca
     2401 aagtccagtc ggcagggcct ttatacattt gtgagcgcgt gcggccatgt atggtaaggg
     2461 ccgctatgcc aatatcttgt aagcgttcgg ctacttcgac aatgttttt
//
LOCUS       DAOMAA010000226         2508 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_36793_length_2508_cov_3.167142, whole genome shotgun sequence.
ACCESSION   DAOMAA010000226 DAOMAA010000000
VERSION     DAOMAA010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36793_length_2508_cov_3.167142"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(679..1857)
                     /locus_tag="PLO59_03935"
     CDS             complement(679..1857)
                     /locus_tag="PLO59_03935"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008245663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HQV00284.1"
                     /translation="MKEVYILSATRTPIGSFNGALASISATKLGAHAIKNAVAQAGIQ
                     PNQVNEVYMGCVLQANVGQAPATQAATFAGLTDITPSTTVNKVCASGMKAIMLAAQSI
                     MLGTNDIVVAGGMESMSQTPYYLDKARNGFRYGNGQITDGIVKDGLFDVYKEYLMGNA
                     AELCATTHNISRAEQDAHAIASYERAQNAYKNGKFKTEISPIEVPVRGKDPMVISEDE
                     EYKNVKLDKVPASKPAFIKDGTVTAANASKLSDGAAALVLISKEKCEALGLKPIGRIV
                     SFADAQQAPEWFTTAPSKALPLALQHAGLSVTDMDYFEINEAFSVVSLANNKLMNLNP
                     DKVNINGGAVALGHPLGMSGARIMVTLLHTLLNENARYGAAGICNGGGGASAMVIERM
                     "
     gene            2052..2468
                     /gene="ndk"
                     /locus_tag="PLO59_03940"
     CDS             2052..2468
                     /gene="ndk"
                     /locus_tag="PLO59_03940"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007846501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HQV00285.1"
                     /translation="MSNITFTMLKPDAVANNYTGAIISDFIQGGFKIQAMKYIWLTAK
                     QAGKFYDIHKERPFYNDLVAYMSSGPIVAMVLEKDNAVESFRTLIGATDPTKAAEGTI
                     RKKYAKSIEANAVHGSDSDANAIIESSFFFSQFERF"
BASE COUNT          754 a          460 c          466 g          828 t
ORIGIN      
        1 cattatttaa taggtattaa agtgttatta tttgtaatag cagcacatgg tttagtagtg
       61 cttacaaacg gggcgcaaaa gagggtatat tttttaagag tagcaaattt tataaccggc
      121 caaacgtgaa agttttacca atgttttcag cctgaaataa acgttaaaac gataaatatt
      181 gcgtggcttt tattgatgtt tggatgcggg ttgagcatgt aggttttcca accaagggca
      241 acatttattg ccttagttgt gcaataccat cttttattgc gatgtggaaa aaatgatatt
      301 gcatgtattg ctaattcggt tgcacacttt ataatttata gaaatcaaat tttattgcgg
      361 ttttaatgga gctatttatt cgcaaggcat ttgggttttt atggaagcac aaaattctaa
      421 ccgatattat agttcacgta tgaattttta taatctcaat tattgtaaaa acactataaa
      481 agtgaaacca taaaaaaatt aatgcaccta acaagcgtga ttatttttat cagtttaaga
      541 aatttaattt ttaataaaaa gaagtaagca aaagtgtgtg ataatttatg tttagttgcg
      601 atgttattta ttgacagcta ataaaaatgc actgccgtag ccgacagtgc atgctttgaa
      661 aattgtttaa atcaaatgtt acatacgctc gataaccatg gcactggcac caccaccacc
      721 attgcaaata ccggctgcac cgtaacgtgc attttcattt agtaaggtat gcagtaacgt
      781 aaccattata cgtgcacccg acatgcccag tggatgacct aatgcaactg cacctccatt
      841 aatgttcact ttatcagggt ttaggttcat cagcttatta tttgctaacg atactacact
      901 aaatgcttca ttaatttcaa agtaatccat atcggtaacc gataagccgg catgttgtaa
      961 agccaaagga agtgctttgc tgggtgctgt tgtaaaccat tcaggcgctt gttgtgcatc
     1021 ggcaaaagat actatacgtc cgataggttt tagtcccaag gcttcgcatt tttctttgct
     1081 tattaaaacc aaagcagcag caccatcgct tagtttactt gcattggctg cagtaacagt
     1141 tccgtcttta ataaaggccg gttttgaggc aggtacttta tctaatttta cgtttttata
     1201 ttcttcgtct tcgctaatca ccattgggtc tttacctcta acaggtactt ctattggcga
     1261 aatttcggtt ttaaatttcc cgtttttgta agcattttga gcacgttcat aagatgcaat
     1321 agcatgtgca tcttgctcag ctctactaat gttatgtgtg gttgcgcaga gttcggccgc
     1381 attgcccatt aaatattctt tatatacatc aaacaaaccg tcttttacaa tgccatctgt
     1441 aatttgtccg ttgccgtatc tgaaaccgtt acgtgctttg tctaaataat agggtgtttg
     1501 gctcatactt tccatgccac ctgctacaac aatatcattt gtgcccaaca taatactttg
     1561 tgcagcaagc ataattgctt tcataccgct agcacaaact ttattaacgg tggtgcttgg
     1621 ggtaatatct gttaagccgg caaaggtggc agcttgtgtt gcaggggcct ggcctacatt
     1681 agcttgtaat acgcaaccca tataaacttc gttaacctga ttaggttgta tgcccgcttg
     1741 cgctacagca ttttttatag catgtgcacc taattttgtt gctgatatgc tggctaatgc
     1801 accattaaaa cttccgatag gcgtacgtgt tgccgataaa atataaactt ccttcatgat
     1861 aatttatttg tagttgatgt tttttataaa taacgcttac taaaaaagcg tgcaagttta
     1921 gcattattta tggttgctta atacagctta aaggctgtta aattaaattg aagacagcat
     1981 tgatttttta ggcattatag gctttgcatt aatttatttt acattcgcgc cctttaaaaa
     2041 aacaaacatt tatgagtaac atcacattta ccatgcttaa gcccgatgcc gtagctaaca
     2101 actacaccgg tgccataatt agcgacttta tacaaggtgg atttaaaata caagccatga
     2161 aatatatttg gcttaccgct aaacaagccg gtaagtttta tgatatacat aaggagcgtc
     2221 ctttctataa cgatttagtg gcttatatga gttcaggccc aatagtggca atggttttag
     2281 aaaaagacaa tgcagttgaa tcgttccgca ctttaattgg tgcaaccgat cctaccaaag
     2341 ctgccgaagg taccatcaga aaaaaatatg ctaaatcaat tgaagccaat gctgtgcatg
     2401 gtagcgatag cgatgccaat gctataattg aaagcagttt cttcttctca caatttgaaa
     2461 ggttttaatt gaaaaccatc aaagtatctg aagcccgatg tagcatat
//
LOCUS       DAOMAA010000227         2505 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_36872_length_2505_cov_2.635510, whole genome shotgun sequence.
ACCESSION   DAOMAA010000227 DAOMAA010000000
VERSION     DAOMAA010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36872_length_2505_cov_2.635510"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="PLO59_03945"
     CDS             <1..215
                     /locus_tag="PLO59_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HQV00286.1"
                     /translation="NTIESYHRNIRKFTKTKAAFTSDNALLKLAFLAIQNMEKIWNKT
                     AFNWKSILSELLITFGERIQIDKLEN"
     gene            303..899
                     /locus_tag="PLO59_03950"
     CDS             303..899
                     /locus_tag="PLO59_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM-DISM domain-containing protein"
                     /protein_id="HQV00287.1"
                     /translation="MLDLASNIETYPITPVVGLYKDTTGRVPFEQIQKQKFKITRKYG
                     LLFPFNDAASWLKIVLTNRNPARKQWVLEWNNPMGESVDFYISKPNGTYQIKKMGNMK
                     DLIGMIPGIGKAVKDIDINNKSFDGIEAIIKSMTPAERIDPSLLNGSRRKRIADGSGT
                     NIQEVNKLIKQFEDMRKMMKMMSGSNMKNMMRNMPVKR"
     gene            968..1558
                     /locus_tag="PLO59_03955"
     CDS             968..1558
                     /locus_tag="PLO59_03955"
                     /inference="COORDINATES: protein motif:HMM:NF019106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUMOD4 domain-containing protein"
                     /protein_id="HQV00288.1"
                     /translation="MATKKVSKAVKPVLTNNKAKSKPVTSLKNEVWKPLKNSDRKYLI
                     SNMGRLIGFYFDKENGKLVKGKTVNGYLAVDIVFNNERKMRYVHTLVAEHFVKRKSPK
                     ASIVIHNNWNKLDNKASNLEWSLPATVVNRTAERNKQYAARMGTVSAKLNREQVSKIK
                     KQLKAGNLQFKIAENFGVSEMQISRIARGLCWANVK"
     gene            1600..>2505
                     /locus_tag="PLO59_03960"
     CDS             1600..>2505
                     /locus_tag="PLO59_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00289.1"
                     /translation="MNFELITEKPLWFIVFCLLLGAAYALLLYHKDNLTALSTSIKRS
                     LMALRFMVVSILAFLLLSPLLKSITRTVEKPLIIIAQDASQSITLGKDSGFYKTEFAE
                     KLNNLQKHLSSNYDVRALNFGSETKPGFDFSYPQKQTDYSILFENLSARFDNRNLGAV
                     IIATDGIYNAGSSLLYASEKLKVPFYTIAMGDTTAKKDLILAKVKHNKTAFLGNTYPI
                     EILIEAKQCAGQNTTLEVLLDSNVIFTKDVNINSNNFNLNVPVYIDAKEKGIQHLKIK
                     LSSIAAELSEANNKTDVFVDVLERKL"
BASE COUNT          904 a          450 c          457 g          694 t
ORIGIN      
        1 caaataccat tgagtcatac caccgaaata tccgcaaatt cactaaaacg aaagcggcct
       61 ttacctcaga taatgccttg ttgaaattag cttttttggc catccaaaac atggaaaaaa
      121 tctggaataa gaccgccttt aattggaaat ctattttatc tgaattgttg attacatttg
      181 gggagagaat tcaaattgac aaacttgaaa attaactgct gacacagttc agtgaaaact
      241 ctcagtatat gaaaaacctc gcctttcttg tttttttgtt accgttatgg ggaagcgctc
      301 agatgcttga tcttgcttcc aatatcgaaa cctacccgat tactcctgtg gtgggtttgt
      361 ataaggatac tactggacgg gttccattcg agcaaatcca aaagcaaaag tttaaaataa
      421 cgcgtaaata cggacttctg tttcccttca acgatgcggc ttcgtggctt aaaattgtat
      481 tgaccaatcg caaccccgcg cgtaagcaat gggtgttgga atggaacaac cccatgggag
      541 agtctgttga tttctatatt tccaagccca atggtaccta ccaaatcaaa aaaatgggaa
      601 atatgaaaga tttaattggc atgatacccg gcattggtaa ggctgtaaaa gatattgaca
      661 ttaacaataa atcgtttgat ggtatagagg ccataataaa atctatgact cctgccgaac
      721 gtatagaccc atcgttatta aacggcagcc gaagaaaacg cattgccgat ggcagtggta
      781 ctaatataca agaggtaaat aaattaatta aacaatttga agatatgcgc aagatgatga
      841 agatgatgag cggcagtaat atgaaaaaca tgatgcgcaa tatgccggta aaaagataac
      901 tgatgttgca gataaattat taattactac gtttacaaca tacgttacaa cctaaatttt
      961 agcatatatg gcaacaaaaa aagtaagtaa agctgtaaag cctgtcttaa caaataacaa
     1021 ggcaaaatcg aaaccggtta cttctttaaa aaacgaagta tggaaacctt taaaaaacag
     1081 cgatcgcaaa tacttaatta gcaatatggg gcgtttaata ggtttttact tcgataaaga
     1141 aaatggcaaa ctggtaaaag gcaaaacagt taatggttat ttggctgtag atattgtatt
     1201 taataatgaa cgaaaaatgc gttatgtaca caccttagtt gccgaacatt ttgtaaaacg
     1261 caaaagccca aaagcaagta ttgtgataca caacaattgg aataaattag ataacaaagc
     1321 aagcaattta gaatggagcc tgcctgcaac cgttgttaac cgcaccgctg aaagaaataa
     1381 acaatatgct gcacgtatgg gtacagtatc cgccaaactt aaccgcgaac aggtttcaaa
     1441 aattaaaaaa caattaaaag ccggcaactt gcaatttaaa attgccgaaa actttggagt
     1501 aagcgaaatg caaatttcac gtatagcacg tgggttatgc tgggcaaatg taaagtaacc
     1561 gatatcagaa aatagattgt aacattaaat catagcgaat tgaattttga actgattacc
     1621 gaaaagccac tttggtttat tgttttttgt ttgttactag gtgcagcata tgcactttta
     1681 ctttatcata aagataattt aaccgcatta tcaacctcca ttaaaagaag tttaatggct
     1741 ttaaggttta tggttgtaag catacttgca tttttactac tctcgccact gctaaagagc
     1801 ataacacgca ccgtagaaaa accactaatc attattgcac aagatgcatc gcaatcaatc
     1861 accttaggta aagactcagg cttttataaa accgagtttg cagaaaaact taataatctt
     1921 caaaaacatc tttcttccaa ctatgatgtg cgtgctttaa actttggaag cgaaaccaaa
     1981 ccaggttttg atttttctta tcctcaaaaa caaacagatt acagtatatt attcgaaaac
     2041 ctttcagccc gtttcgacaa cagaaacctg ggcgctgtta ttattgctac tgatggtatt
     2101 tacaatgcgg gaagcagctt gctatatgca agcgaaaagt taaaagtgcc gttttatacc
     2161 attgcaatgg gcgataccac agcaaaaaag gatttgatac ttgcaaaggt taaacacaat
     2221 aaaacagcat ttttaggcaa cacctacccc attgaaattt tgattgaagc caagcagtgt
     2281 gccggacaaa atacaacctt agaagttttg ctggatagta acgtgatatt tacaaaagac
     2341 gtaaatatta atagcaataa tttcaacctg aacgtacctg tttatattga tgccaaagaa
     2401 aaaggcatac agcatttaaa gattaagcta agcagcattg ctgccgaact tagcgaagca
     2461 aataataaaa ccgatgtatt tgttgatgta ctcgaacgca aatta
//
LOCUS       DAOMAA010000228         2502 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_36953_length_2502_cov_3.030241, whole genome shotgun sequence.
ACCESSION   DAOMAA010000228 DAOMAA010000000
VERSION     DAOMAA010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36953_length_2502_cov_3.030241"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1074)
                     /locus_tag="PLO59_03965"
     CDS             complement(<1..1074)
                     /locus_tag="PLO59_03965"
                     /inference="COORDINATES: protein motif:HMM:NF013604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00290.1"
                     /translation="MKRKLKIYGALKPIGLALMGWSLCTAALAQSVNPLLQSIVKPSS
                     TQHWITFNEAGSNLNANRLFDEYKAGFGLTAADEMKLDQTKSDEIGMQHYNFKQRHQG
                     YEVMFGTFKVHQAADGKLTSANGRIITNIRNQASAVLTETQALQKALAFMGAKKYLWQ
                     NDALENELKRTEENSQATYFPKGELQYTPNNFDGTFKGEDYRLGWTFKIYTDDREVSA
                     KFVAIDALTGNVNYYHDISMSCVTGSGTTAWQGSRTLHTRLSGGTHQSHNDCQTTDLI
                     VYNCNGNGATNTYYTDANNVWTATTQQSAAQAQFGIEGTYDYFNGVHARQSWNGSSGD
                     MIAYNNAFAGSNNACWGCTGNSTI"
     gene            complement(1117..>2502)
                     /locus_tag="PLO59_03970"
     CDS             complement(1117..>2502)
                     /locus_tag="PLO59_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00291.1"
                     /translation="NASMSFKSTTGTANMILDRANNTATASVSYRTNGAPTFQTGTVG
                     TDNFVIRNIFLGAPAFCVVQSNNNVGIATNTPSASFSVNNKLLVDAVNGDMSFSDQLG
                     SITFPAVSGAADAMINMFASGIGNGDRMVIAHSPGFSTYGLQYQDATDKFNFIGNGSN
                     VLTADLANLRVGIGTNAPTTKLHVVGASTLAGNTAVTGTLNVSGATTIGGILDVNGDT
                     IRFGSVERLIDGGANTIATNSTFRPETDLGANLGSTIYRWNNVFAGNSVSIGGTSTVL
                     PLHVDGGTDVGLASGGYACFGATTAENVTIDNNEIMARNNGAASSLFVQHSGGNTVIN
                     ANGGAVCIGIGTPATGYLVSVDGKLIAEEVRVELSGNWPDYVFSNDYKMLSIEELENH
                     IATYNHLPNVPSAAEVAADGIMLGEMQTKTMEKVEENALYIIELNNKLKAQDQLIQKL
                     LKEVEALKNDK"
BASE COUNT          687 a          546 c          476 g          793 t
ORIGIN      
        1 aatagtagaa ttaccggtac aaccccagca agcattattg ctaccggcaa atgcattgtt
       61 ataggcaatc atatcaccgc tgcttccatt ccaactttga cgcgcatgta caccattaaa
      121 atagtcgtaa gtaccttcaa taccaaactg tgcttgtgca gcactttgtt gtgttgtagc
      181 tgtccagaca ttgtttgcat cggtgtaata tgtattggtt gcgccattac cattacagtt
      241 gtacacaatc aaatcagttg tttggcagtc gttatgcgat tgatgggtac caccgcttaa
      301 gcgtgtatgt aaagttctgg aaccttgcca ggctgttgta ccactaccgg taacacaact
      361 cattgaaata tcgtgatagt agtttacatt acctgttaag gcatcaatag caacaaattt
      421 agcggacacc tctctgtcgt cagtgtaaat tttaaacgtc cagcctaagc gatagtcttc
      481 gcctttaaat gtaccgtcaa aattgttggg tgtgtattgt aattcgccct taggaaaata
      541 agtagcctgg ctgttttctt cggtacgttt taattcgttt tccaacgcat cgttttgcca
      601 caaatacttt ttggcaccca taaatgctaa tgctttttgc aaggcttgtg tttcggttaa
      661 cactgccgat gcctggtttc tgatgttggt aatgatacgg ccgtttgccg aagttaattt
      721 gccatcggct gcctgatgca ccttaaatgt gccaaacatt acttcatagc cttgatgcct
      781 ttgcttaaag ttgtaatgct gcatgccaat ttcatcgctt ttggtttgat cgagtttcat
      841 ttcatcggca gcagttaagc caaaaccggc tttatattca tcaaacaagc ggttggcatt
      901 taagtttgaa ccggcttcgt taaacgtaat ccagtgttgt gtgctgcttg gctttacaat
      961 gctttgcagt aatggattta cgctttgcgc taaagcggct gtacataaag accatcccat
     1021 tagtgctaag cctataggct ttaaggcacc gtatattttt agttttcttt tcatagcttt
     1081 tgtttttagc agttaaacat tatgtgtatg aaaaaattac ttatcgtttt ttaatgcttc
     1141 aacttctttc aatagttttt gaataagttg gtcctgagct tttagtttat tatttaactc
     1201 gataatgtat aaagcgtttt cttcaacttt ttccatggtt ttggtttgca tttcgccaag
     1261 cattattccg tctgctgcta cttcggctgc cgatggtaca ttgggcaaat gattgtaagt
     1321 tgcaatatga ttttccaact cttcaatact caacattttg taatcgttgc taaatacata
     1381 gtcaggccag ttgcccgata actcaacacg tacttcttct gcaatcagct tgccatcaac
     1441 agaaactaag taaccggtag caggcgtgcc tataccaatg caaacagcac caccatttgc
     1501 attaattacg gtattgccac cactgtgttg cacgaacaaa gacgatgctg caccattgtt
     1561 acgtgccata atttcgttgt tatcaattgt tacgttttcg gcagttgtag caccaaagca
     1621 tgcatagcca ccactggcta agcctacatc tgtaccacca tcaacatgca atggtaaaac
     1681 agtacttgtg ccaccgatag aaaccgaatt acctgcaaaa acattgttcc aacgatagat
     1741 agttgatcct aaatttgcgc ccaaatcggt ttcaggtctg aaagttgaat tggttgctat
     1801 ggtgtttgca ccgccatcaa ttaagcgctc aacagaacca aaacgaattg tgtctccgtt
     1861 aacatccaat atgcctccaa tagtggttgc acccgataca tttaatgtac ctgtaaccgc
     1921 agtattacct gctaatgttg atgcgccaac tacatgtaat ttggtagttg gtgcattggt
     1981 accaatacct acgcgcaagt tagctaaatc ggcagttaat acattgctgc cattaccgat
     2041 aaaattaaac ttatctgtag catcctggta ttgcaaacca taggtagaaa aacctggcga
     2101 atgcgcaata accatcctgt caccattacc aattccactg gcaaacatat ttatcatggc
     2161 atctgcagca ccactaacag ccggaaatgt tatcgaaccc aattggtcgc tgaaactcat
     2221 atcaccgttt acggcatcaa ccaataactt gttgtttacc gaaaacgaag cagaaggtgt
     2281 attggtagca ataccaacat tgttgttaga ttgtactacg caaaaggcag gcgctcctaa
     2341 aaatatgttt ctaatcacaa aattatcggt acccacagta cctgtttgaa atgttggtgc
     2401 accgttggta cgataactta ccgaggcagt tgctgtattg ttagctctgt ctaaaatcat
     2461 attggctgta cccgtagttg acttaaacga catactggca tt
//
LOCUS       DAOMAA010000229         2501 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_36982_length_2501_cov_3.066231, whole genome shotgun sequence.
ACCESSION   DAOMAA010000229 DAOMAA010000000
VERSION     DAOMAA010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36982_length_2501_cov_3.066231"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..1662
                     /locus_tag="PLO59_03975"
                     /pseudo
     CDS             1..1662
                     /locus_tag="PLO59_03975"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
     gene            complement(1659..>2501)
                     /locus_tag="PLO59_03980"
     CDS             complement(1659..>2501)
                     /locus_tag="PLO59_03980"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQV00292.1"
                     /translation="AQLVPISVYPNMISWSSSAWYMGAIMGPAIGGLIYAWFNPAVAC
                     FTFTALMFLAMCCYVMIASKPVANKLTEPLMQSLKSGIVYVYRNKILLSAITLDLFAV
                     LFGDAIVLLPAFANDVLHIGAKEMGYLRAAPALGALAMAVVLAYHPPLKNTGKKLLLA
                     VAGFGLCMIVFALSKMFVLTFIILVVAGMLDNISVMIRSTILQLQTPDNMRGRVASIN
                     SIFIGSSNEIGSFVSGSMARAFTLIPSVIIGGLASITVVAVTTWKAPKLLNYEIMERG
                     NKLN"
BASE COUNT          835 a          483 c          469 g          714 t
ORIGIN      
        1 tacgatccgg ctttcagcgg ttatatatac atacaagaca atacatggcg catacacagt
       61 ttagatttat acctgagtaa aaataaccag ctacagtttg ttgatacgtt taaagtaaat
      121 caggtaatgt tgcctgcaaa tcaaaacagt aatatatgga tgccggtaac caataccttt
      181 tattatgcct ttaaaatttt tggtttcgaa ggcgatggcg tttatacagg cattgccagt
      241 aattacgaag taaacccaaa ttttgaaaca aaatttttta atggtgatgt tattaccatt
      301 gaagaaggca gcaataaaaa agatagtttg tactggaacg ccatcaggcc aataccactt
      361 acacaattag agcaaaagga ttatctgttc aaggattcgg ttgctgttat aaaagaatcg
      421 aaaccttata tggattcagt ggatgccgta cataacaaat tagatgttat gaaattgctt
      481 atgagcggct atcagcacaa taaccgttac aaaaagttta aatggggttt agcaccgcta
      541 atggaacata ttgtatttaa taccgttgag ggtcttaata ttggtggtgc atataacttt
      601 acaaaacgtt acgaagttcc gaaacgaaca ctttatggca aagcagcagt acgctatgca
      661 tttgctgccg ggcaggtata tggccaggca tcggtaaact actacttcaa tcgtaaaaat
      721 ttttcagaca tcggttttgt agccggcagc gatgccgtgc aatacaatcc gcgcaaccca
      781 atacgcgcat cggttaactc attttatacc ttagcattag aaaaaaacta tatgaagctg
      841 tatcagaaag attttgcgca gctaaacttt aaacgcgaaa ttgccaaggg attatttttt
      901 gcttcactac tcgaatatag ttggcgcaaa gcattggtaa acaacacaac ttatgtaatg
      961 tttccgtctg atgcacgtag ttatagcagt aataacccac aaaacagtac cgatgatgcg
     1021 cctgcatttg atacacatca atcattacat tttgcaggtt tggtacgcta tacgtttcgc
     1081 caacaatatt acaacgaccc tgaaaatcgg cacatgattc aatcgcctta tccggtggcc
     1141 gtatttcgct ttaagcaagg gattccaaaa acatttggta gtgatgtaaa ctacacctat
     1201 gtgcatttaa gtatcgaaga cgaattaaaa ctgggcatgt taggtaatgt gcaaatcaat
     1261 gtaggagcag gtaaattttt aagcaataaa tctgattaat ttttagacta ccgtcatttt
     1321 aatggcaata aaacgtatgc ttccgagttc agactaaccg attttaatat gctgccctat
     1381 tatagttata gcacaacagg tgcgttttac gaagcacatg ccgagtacaa cttaggcgga
     1441 ctattgttta acaaaatacc ggttttacgt aaagcatttt ttaatgagat agtaagtgcg
     1501 cattatttaa aagtacctaa taatccacaa cactttgaat ttgcagtagg tgttgaaaaa
     1561 ttaggtgcca tcagattaga tgtagtaagt gccgtagcca aaggctttaa gccggtagta
     1621 ggtgttaggt ttggtgttcg tggtggcccc tttggcgatt aatttaattt attgccacgc
     1681 tccattattt cgtagttcaa taatttaggt gctttccagg tagtaacggc taccactgta
     1741 atgctggcta aaccgccaat aattaccgat ggtatcagcg taaaagcacg tgccatacta
     1801 ccactaacaa aactgccaat ttcatttgat gagccaataa aaattgaatt gatggatgca
     1861 accctgccgc gcatattatc aggtgtttgc aattgcagaa tagtacttct aatcatcaca
     1921 ctaatgttgt ctaacatacc ggcaactact aagataataa aagtgagtac aaacattttc
     1981 gaaagggcaa aaactatcat acataaacca aaaccggcaa ctgccaacaa taattttttg
     2041 cctgtatttt ttaaaggtgg atggtaggcc aacacaactg ccatagccaa agcacctaat
     2101 gccggtgccg cacgtaagta acccatttct ttagccccaa tatgcaaaac atcgtttgca
     2161 aaggcaggca acaatacaat ggcatcgcca aacaatacag caaacaaatc gagtgtaatt
     2221 gcacttaata atattttgtt tcgataaaca taaacaattc ctgatttaag gctttgcatc
     2281 aatggctctg ttaatttatt agctacaggt tttgaagcaa tcattacata acaacacatg
     2341 gccaaaaaca tcaaggcggt aaaagtaaaa caggctacag ccggattaaa ccaggcatat
     2401 attaatccac caatagcagg acccatgata gcacccatat accaggcact gctactccat
     2461 gaaatcatat ttggataaac acttatgggt actaattgtg c
//
LOCUS       DAOMAA010000230         2490 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_37295_length_2490_cov_2.031211, whole genome shotgun sequence.
ACCESSION   DAOMAA010000230 DAOMAA010000000
VERSION     DAOMAA010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37295_length_2490_cov_2.031211"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2490)
                     /locus_tag="PLO59_03985"
     CDS             complement(<1..>2490)
                     /locus_tag="PLO59_03985"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HQV00293.1"
                     /translation="GKSFVTDSTSNGLANNHVWYITEDRHHNIWIASNGGGASCFDGK
                     SFKTLNEGNGLISNYVKCILEDRAGHIWMATDKGITKLVAPYKGLNFANSISVCKKIS
                     MPFSDGVTSMAQSNDGNIWCGTIQGAVCIDANTNGAELKIKSTYTIANGLPNNYVTSL
                     YNDSKGKLWLSTIAGLSMLAKSDHANLEDIDKNKFINYNKSNGLIDNNITSIAEDLEG
                     NIWLTSNQGVSCFNQYKFKSYTTANGLLSNYIKNITCDKQGNLWFATYGLGACCYNGN
                     TFEFGTTNEGLIDNNITCLNNYFNGSLWIGTNAGFSVFNDNDTTKPPMFTNYGTMQGL
                     HGNLVRCIFQSKNDHVWITTIDSGLSVFEVNSLHANSKLKATYSKSGSITYNHQVMQS
                     TALATFNINVGNSKLNLRTIFEDKAGNIWIACNNGLLFHSKANDTYPDYNEAINKLKM
                     VCYTKAQGLPDNLIYSITEDHTGNLWFASQKNGVSKYDGNRVSAIKNGNTPDNTTGLK
                     KINQKFAATFTNYNTKQGLGHNCVFKVRTDTCNNIWLATYGGGLSRYHPSEKGKLFLN
                     FTTDNGLADNAVYDFVFDHSGNIVAGTNLGFSVLTHFFLKKQYKASSALPPNLPVINN
                     LNNFDLEKYYQPVFEIFNKHTGYPIKDIVNGSMYCDSNGIVWAGSGDDKLIRFNRSAA
                     LKIKLPPQVAIQQIKINDENICWSCIDGNNLYNQTTQSNEQMLVYGKQLNTFQADSIK
                     HTYKQISFDSVAPFYQIPYNLELDYKHNQITFDYTTIEPASASLAKYQYILEGYSNGW
                     HAITNKTTATFGNMHEGTYTFKLKALDTQGQW"
BASE COUNT          706 a          466 c          446 g          872 t
ORIGIN      
        1 ccattgcccc tgggtatcaa gcgctttaag tttaaaagta taggtgcctt catgcatatt
       61 gccaaatgtt gcagtagttt tattagtgat ggcatgccag ccattactat acccttcaag
      121 tatatattga tattttgcca acgatgccga tgccggttcg atggttgtgt aatcgaacgt
      181 gatttgatta tgcttatagt ctaactccag attataaggg atttggtaaa acggggcaac
      241 actatcaaag ctaatttgtt tgtaagtatg tttaatagaa tcagcttgaa acgtatttag
      301 ctgtttgccg taaaccagca tttgctcatt ggattgtgtt gtttggttgt ataagttatt
      361 gccatcaata cacgaccagc aaatattttc atcatttatt tttatttgtt gaatggctac
      421 ctgtggcggt aacttgattt ttaatgcagc acttctgtta aatcgtatca gcttatcatc
      481 gccactgccg gcccaaacga tgccattgct atcgcaatac atcgaaccat ttacaatgtc
      541 ttttatcgga tagcctgtat gtttattaaa tatttcgaaa acgggctgat agtatttttc
      601 taagtcaaaa ttgttcaggt tgttaataac gggtaaatta ggtggcagtg cagagcttgc
      661 tttatactgt tttttcagaa aaaaatgcgt aagaaccgaa aaccccaaat tagttccggc
      721 aacgatattg ccactatggt caaatacaaa gtcatatact gcgttatcgg ctaaaccgtt
      781 atcagtagta aagtttaaaa acaatttacc tttttcagat gggtggtacc ggcttaaacc
      841 gcctccgtat gttgcaagcc aaatgttgtt acaggtatcg gtgcgcactt tgaaaacaca
      901 attatggccc aggccctgct tggtattgta attggtaaaa gttgccgcaa atttttgatt
      961 tatttttttt aaaccggtag tgttgtcagg tgtgtttccg ttttttattg cactaaccct
     1021 gtttccatcg tatttgctta ctccattttt ctggctggca aaccaaaggt taccggtatg
     1081 gtcttccgtt atcgaataaa ttaaattatc gggcaaacct tgcgctttag tatagcatac
     1141 catttttaat ttattgatgg cttcattata gtccgggtag gtatcgttgg ctttactatg
     1201 aaacaataat ccattgttac atgcaatcca aatgttgccg gccttgtctt cgaaaatggt
     1261 tcttaaattt agttttgagt taccaacatt aatattgaat gttgctaaag cagttgattg
     1321 cataacctga tggttatacg taatagagcc tgatttgctg taagttgctt ttagctttga
     1381 attggcatgt aaggagttaa cttcaaatac agacaggcca ctgtctatgg ttgttatcca
     1441 tacatgatcg tttttgcttt gaaatatgca gcgaaccaga ttgccatgca aaccttgcat
     1501 agtaccatag tttgtaaaca tgggaggctt ggtagtatcg ttgtcattga aaaccgaaaa
     1561 acctgcattg gtgccaatcc acaaactgcc attaaagtag ttatttaaac aagtaatgtt
     1621 attgtcaatt aatccttcat tggttgtacc aaattcaaat gtattgccat tataacaaca
     1681 agcacctaag ccataggttg caaaccataa attgccttgc ttatcgcatg ttatattttt
     1741 tatataattt gataataaac catttgctgt agtataagat ttaaatttat actgattaaa
     1801 acagcttaca ccctggttgc ttgttaacca gatatttcct tccaaatctt cggcaatgga
     1861 agtaatgtta ttatcaatca ggccatttga tttattatag tttataaatt tgtttttatc
     1921 aatatcttcc agattagcat ggtcgctttt tgccaacatg ctaagccccg caatagttga
     1981 caaccataat ttgcctttgc tatcattata caatgatgta acgtaattat tcggtaaacc
     2041 gtttgcaata gtgtaagtgc ttttaatttt caattcggca ccgtttgtat ttgcgtcaat
     2101 acaaactgcg ccttgtatcg taccacacca aatattgcca tcattacttt gtgccatact
     2161 tgtaacccca tcagaaaatg gcatgcttat ttttttgcaa acgcttatgg aatttgcgaa
     2221 gtttaggcct ttataaggcg ctacaagttt ggtaatgcct ttatcggttg ccatccaaat
     2281 atggccggct ctatcttcta aaatacattt tacataatta ctaatcaagc cgtttccttc
     2341 gttcaaagtt ttaaacgatt tgccatcgaa acaacttgca ccaccaccat tactggcaat
     2401 ccaaatatta tggtgtctgt cttcggttat gtaccaaaca tggttgtttg ccaatccatt
     2461 acttgtacta tcggttacaa atgatttacc
//
LOCUS       DAOMAA010000231         2485 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_37430_length_2485_cov_3.463786, whole genome shotgun sequence.
ACCESSION   DAOMAA010000231 DAOMAA010000000
VERSION     DAOMAA010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37430_length_2485_cov_3.463786"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="PLO59_03990"
     CDS             complement(<1..254)
                     /locus_tag="PLO59_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HQV00294.1"
                     /translation="MSTLQSHSAHHKLSLAGLLVTLGIIFGDIGTSPLYVMKAAIGQE
                     AINEEIIYGVISCILWTLTLQTTFKYVVLTLQADNRGEGG"
     gene            381..>2485
                     /locus_tag="PLO59_03995"
     CDS             381..>2485
                     /locus_tag="PLO59_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00295.1"
                     /translation="MNSFIDLLIQKLIETKIPFNEMQVVFPTKRACILFKYKFALHFK
                     RPQWLPQTSSIEDFILNLSALEKANDLFLITQLFEAYKKFDSQIEFDSFYNWGKILLA
                     DFDETDSYLIDQNKLYVTLQNFAEVDNSFGMNEEEWQSLISFWKSIYNGSPTKLRQQF
                     INSWKILPDVLNEFKQNLYAAGKVYRGLAYRTVAENIQTQKLSHTVFAGFYALSTSEE
                     YIIKYLLDNNMASMFWDADAYYLNNPIQEAGYYLRNNLLLHERYRNNSTILKTDTGLS
                     FADEKEINLTGIPLKVGQAKFTGNKLKTLIDTNQLDDAKTVIVLPDEHLLTPMLHALP
                     FIENVNVTMGYPIKHTQVYSWLNIYIQCLQNRSNESVFFKDDLMLIFNHPLAGNYLGN
                     KQHEFIDRLNASVTTLININKLNTIAENHILQLLCMPTINMFECVAATSAFIDFLFIN
                     LSSALNTIEHEALVNLQNQFKQQFETVVNNASLSTKSAWLLIADTIKNLRIPFEGKAA
                     TGLQIMGFLETRVLDFDRVILLSANENYLPAAKASSSYIPYNLRKAFGLPTAENQNAI
                     YAYHFYRLLQRARYIDILYNTELNRTGGGEPSRYLLQIKYELLKTNPAIKFTDSIYTT
                     PIHSIEQNAITIYKTAAIENKLKLKYVYTNGNTNQFSASSLSTYIKCKLAFYFKYIIE
                     IKTETETTDTIDAATFGNI"
BASE COUNT          883 a          436 c          396 g          770 t
ORIGIN      
        1 atgcctcctt cacccctgtt gtcagcttgt agtgtaagaa ctacgtattt aaaagttgtt
       61 tgtaaagtga gcgtccataa tatacatgat attactccgt atataatttc ttcgttaatg
      121 gcttcctgcc caatagctgc cttcatcaca tatagtggtg aggtaccaat atcgccaaaa
      181 ataattccga gggtaactaa taagcctgcc agagaaagct tgtgatgcgc tgaatgtgat
      241 tgcaaggtgc tgatgctaaa acaactgcaa acctatttat taataaacat ttgcacaaat
      301 tatttttaat tacatctttt tagctccaaa tttcaatttt aaatcataac cacatttttg
      361 gcgaaaccat cttatacagc atgaattcat ttattgattt actgattcaa aaactaatag
      421 aaacgaaaat tccgttcaat gaaatgcaag tggtgtttcc aactaaacgc gcatgcatat
      481 tgtttaaata taaatttgca ttacacttta aacggccaca atggttgccg caaacaagta
      541 gtattgaaga ctttatttta aacctatcgg ctttagaaaa agctaacgac ttgtttttga
      601 ttacacaatt gtttgaagcg tataaaaaat ttgatagcca aattgagttt gatagttttt
      661 acaattgggg gaaaatttta ttagctgatt ttgacgaaac cgacagctat ttgattgatc
      721 aaaacaagct atatgtaaca cttcaaaatt ttgcggaagt tgataattcc tttggcatga
      781 acgaagaaga atggcaatca ttaatttcgt tttggaaaag tatttacaat ggtagcccaa
      841 ctaaactaag gcaacaattt ataaatagct ggaaaatatt acccgatgta ctaaatgaat
      901 ttaagcaaaa tctgtatgca gcaggtaagg tatatcgtgg tttggcttat cgaacagtgg
      961 ctgaaaatat acaaactcaa aaactcagtc acactgtatt tgcaggtttt tatgcattaa
     1021 gcacatcgga agaatacata atcaagtatt tacttgacaa taatatggcc tccatgtttt
     1081 gggatgcaga cgcttattat ttaaacaatc cgattcaaga ggctggttac tacttgcgta
     1141 ataacttgct tttacatgaa aggtacagga ataattcaac cattttaaaa accgatacag
     1201 gcttgagttt tgctgatgaa aaagaaatca accttacagg tattccttta aaagtaggcc
     1261 aggcaaaatt taccggaaac aagttaaaaa cacttattga tactaatcag cttgatgatg
     1321 ctaaaacggt tattgttttg cctgacgagc atttattaac gcccatgttg catgcattac
     1381 cttttattga aaatgtaaat gttaccatgg gataccccat aaaacacaca caagtttata
     1441 gctggttaaa catttacatt caatgtttac aaaacaggag caatgaatcg gttttcttta
     1501 aagatgattt gatgttaatt ttcaatcatc cattagcagg caattatctg ggaaacaaac
     1561 agcatgaatt tattgatcgc ttaaatgcaa gtgttactac tttaattaac ataaataaat
     1621 taaacaccat agccgaaaac catattctac agctattgtg catgccaaca ataaatatgt
     1681 ttgaatgtgt tgctgctact tcagcattta ttgactttct ttttataaat ctaagttcag
     1741 cattaaacac cattgagcat gaagcccttg taaatttgca aaaccagttt aagcaacaat
     1801 tcgaaacggt tgtaaacaat gcatctctat caaccaaatc ggcctggttg ctgatagcag
     1861 atacaattaa aaatttacgc ataccattcg aaggcaaagc ggccaccggt ttgcagatta
     1921 tgggattttt agaaacacgc gttttagatt ttgacagggt aatattatta tcggcaaacg
     1981 aaaactattt accggcagca aaagcttcaa gcagttatat tccatacaat ttgcgtaaag
     2041 catttggatt accgacagca gaaaatcaaa atgcaattta tgcataccat ttttacaggc
     2101 ttttacaacg tgcccgttac attgacattt tatacaatac cgaattaaat agaacaggtg
     2161 gtggagagcc cagccgctat ttattacaaa tcaaatacga acttttaaaa acaaaccctg
     2221 caataaaatt taccgatagc atttacacca ctcctataca tagtatcgaa caaaatgcaa
     2281 taactattta taaaaccgct gccattgaaa ataaactaaa attaaaatat gtttatacaa
     2341 atggtaatac caatcagttt tcagcatcat cgctcagcac atacatcaaa tgcaaactgg
     2401 ctttttattt taaatatata attgaaataa aaacagaaac tgaaaccact gatacaattg
     2461 atgcggctac gtttggaaac attat
//
LOCUS       DAOMAA010000232         2481 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_37558_length_2481_cov_2.546991, whole genome shotgun sequence.
ACCESSION   DAOMAA010000232 DAOMAA010000000
VERSION     DAOMAA010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37558_length_2481_cov_2.546991"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2301
                     /locus_tag="PLO59_04000"
     CDS             <1..2301
                     /locus_tag="PLO59_04000"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HQV00296.1"
                     /translation="KSNLTYPGKNLANIWGYVDTLGNEYALVGTSAGLSIVNVTNPVA
                     PVEVFTVAGTTSAWREIKTHGKYAYVTTEGGTLGLQIVNLSNLPASISSKYYKGDGAI
                     NNLLNKAHALHIDNGYCYLYGTNLFSGAAVILNLNDPWNPVYAGNTSAVGVTSNYIHD
                     GCVFNDTLWGSHIYGGYFSAIDVSNKATPVLLETQNTPTNFTHNTWLSDNHRTLFSTD
                     ENSNSYLAAYDVSDVNNITLLDKIQSTPGNGAVIHNTYVHNDYAVSSYYTEGIVVVDA
                     AKPDNLIKVGQYDSSPAYSGSGFNGCWGVYPYLPSGNLIVSDIQTGLHVVTPNYVRGC
                     YLEGKVTSSYNGLPIIGANVTVAGPGLNETTNATGDYKTGTHQSGTYTVTVTKVGYVT
                     KTVSVTLANGVLSLLNVALDLPGIAITGTIKNQYTNAGVANCEILLQSNYNTYTGLTD
                     SNGDLMINNVSPGNYNYACGVWGYKTKCGTILLDGNNNVLNQNLYPGYADDFTFDFNW
                     KTQNSLTTGAWVRQQPVGTTYPVANDANPNTDVNNDCFDKCYITGNGGGAASFDDVDS
                     GYTKLTSPNIDFTVYADPVIQYSRWFFASPGSKDTLIVRLTKGNTTVELERIDINTPG
                     KSAWVDKSFRLLDYMTKGKKIKLSFYVRDKNADHVIECGVDNFMVYENFLPVVSTHKV
                     VTNNNDLIVKAYPNPLTDVLNIKLNQSGAFTFEIRDITGRLIMSNIIDSKSGIYELDC
                     SLLNKGMYLLHISNQNTNAVLNIVKH"
BASE COUNT          765 a          435 c          497 g          784 t
ORIGIN      
        1 aaaagtaacc ttacttatcc gggaaaaaat ttagcaaaca tttgggggta tgtagatacg
       61 ctgggtaatg agtatgcatt ggtgggtacc agtgcaggtt tgtcaattgt aaatgttacc
      121 aatccggttg caccggtcga agtgtttacc gttgccggta ccacatcagc ctggcgcgaa
      181 attaaaaccc atggcaagta cgcttatgta acaacagaag gaggtacact aggcttacaa
      241 attgtaaacc tgagcaattt accagcaagc ataagcagta aatattataa aggcgatggc
      301 gctattaata atttactcaa taaagcacat gcactgcata ttgataatgg atactgttat
      361 ttatacggta ccaatttgtt ttcgggtgca gcggttattt taaatttaaa cgatccatgg
      421 aatccggttt atgccggtaa tacaagtgcc gtaggtgtta ccagtaatta tatacatgat
      481 ggttgtgtgt ttaatgatac tttgtggggc tcacatattt atggaggtta tttttcggca
      541 attgatgttt caaataaagc cacaccggtt ttattggaaa cacaaaacac acctacaaat
      601 tttacacaca atacctggtt aagcgataac caccgtacct tattttctac agacgaaaac
      661 tcgaactcat acttagctgc ttatgatgta agtgatgtga ataatattac gttgttagat
      721 aaaattcaat caacacccgg taatggtgct gttattcata atacctatgt gcataacgat
      781 tatgcagtaa gctcttacta taccgaaggt attgttgtgg tagatgcagc caagcccgat
      841 aaccttatta aagtaggtca gtatgattca tcgccagcat acagtggtag tgggtttaat
      901 ggctgctggg gtgtttatcc gtatttacca tcaggcaatt taattgtatc tgatattcaa
      961 actggtttac atgttgttac accaaactat gtgcgcggct gctatttaga aggtaaggtt
     1021 acttcgtctt ataacggttt gcccattatt ggtgcaaacg ttacagttgc cggaccgggc
     1081 ttaaatgaaa ctaccaatgc aacaggcgat tacaaaaccg gtacacatca aagtggtacc
     1141 tatacagtaa cagttactaa agttgggtac gtaaccaaaa cggtttcggt tacacttgcc
     1201 aatggtgtac tttctctact taatgttgct ttagatttac cgggtattgc tataaccggt
     1261 acaattaaaa atcaatacac caatgcaggt gttgccaatt gtgagatttt acttcaaagt
     1321 aattataata cctacaccgg tttaaccgat agtaatggcg acctgatgat aaacaatgta
     1381 tcacccggca actacaatta tgcatgtggt gtttggggtt ataaaactaa atgtggaact
     1441 attttgctgg atggcaacaa caatgtacta aatcaaaacc tttatccggg ttatgctgac
     1501 gattttacct ttgattttaa ctggaaaact caaaattcgt taaccacagg tgcatgggta
     1561 cgtcaacaac ctgttggtac cacttatcct gttgccaatg acgccaatcc aaataccgat
     1621 gtaaataatg attgttttga taagtgttat ataaccggca atggtggtgg tgctgccagt
     1681 tttgatgatg ttgattcggg ctacaccaaa ttaacttcgc ctaatattga ctttactgta
     1741 tatgccgacc ctgtgataca atatagccgt tggttttttg cttcaccggg tagtaaggat
     1801 acgcttatag tccgcttaac aaaaggtaat acaactgttg agttagaacg tattgatatt
     1861 aatacaccgg gcaaaagtgc ttgggttgat aaaagtttcc gtttattgga ttatatgact
     1921 aaaggcaaaa aaataaaatt gtcgttttat gtgcgtgata aaaatgccga ccatgtaatt
     1981 gaatgtggtg tagataactt tatggtttat gaaaactttt tgccggttgt ttctacccat
     2041 aaggtagtaa ccaataacaa tgatttaata gtaaaagcat atcccaatcc gctgacggat
     2101 gtattaaata ttaaattgaa tcaaagcggt gcatttactt ttgaaatacg tgatattacc
     2161 ggccggctta taatgtcaaa tataattgac agcaaatcag gtatatatga gcttgattgt
     2221 agtttgttaa acaagggcat gtacctttta catatcagta atcaaaatac aaatgcggtt
     2281 ttaaatattg taaaacatta aggttaagca agcaaaaaaa agggtgcaaa atgttgcacc
     2341 cttttttttg cttgcactaa tacaaaatgt caacgcttgc ttctaatctt actgccgtgg
     2401 ctaccgcttt tctttttagc aggatttgct tttactggtg gtttttggat tactgccaat
     2461 aaactatcgg ttgctgcttt c
//
LOCUS       DAOMAA010000233         2477 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_37665_length_2477_cov_2.939719, whole genome shotgun sequence.
ACCESSION   DAOMAA010000233 DAOMAA010000000
VERSION     DAOMAA010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37665_length_2477_cov_2.939719"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            50..1270
                     /locus_tag="PLO59_04005"
     CDS             50..1270
                     /locus_tag="PLO59_04005"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020596322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HQV00297.1"
                     /translation="MIRRFDVAAKILNLEADIYDVMKYPAKNIEVNLPIMMDNGLIKV
                     FNGYRVIHSTALGPSKGGIRYSMDVNEDEVKALAAWMTWKCAIADIPYGGAKGGIKCD
                     PSKMSVGEIERLTRAYTKAMSDIFGVDKDIPAPDMNTGPREMAWIVDEFSNLKGGFTP
                     GIVTGKPLHLGGSLGRVEATGRGVNTSAICAMNKMKLNPKKSTLAVQGFGNVGSISAR
                     FLVEQGLKLVAISDYTGAYYNPKGIDLAKALTYRNSNKGNLEGFKGGSKLTNDDLLCL
                     KVDVLAPCALENQITKDNAANVQAKLIVEGANGPTTSAADDILNKKGVIVVPDILANG
                     GGVTVSYFEWVQNRTGYYYSEEEINKRANNWMTKAFENVWSVAQKHKTSLRIAAYVYA
                     MNKVATAIKSRGSY"
     gene            1361..2020
                     /locus_tag="PLO59_04010"
     CDS             1361..2020
                     /locus_tag="PLO59_04010"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HQV00298.1"
                     /translation="MTIHKEGYRTITYTALFCLLVNVIVFYFFPMHDFLRIASLVVTV
                     ALFLIILQFFRLPARKVKRNDSLVLAPCDGKVVVIEEVFEAEYYQAKRLQVSIFMSPI
                     NVHANWYPISGIVKYVKYHTGKYLVAWDPKSSTENERSTIVVEKENKVSVLFRQVAGA
                     LAKRIIYYPHEGDMVKQGAEFGFIKFGSRVDIYLPLNAKVKVKIGDKSTGGITEVAEI
                     E"
     gene            complement(2079..>2477)
                     /locus_tag="PLO59_04015"
     CDS             complement(2079..>2477)
                     /locus_tag="PLO59_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQV00299.1"
                     /translation="FILFYFVGGYLLYSALFAAIGAAVDSETDVQQFMIPITIPLIFA
                     YVASTIVMQNPDSSMGFWFSIIPLTSPIVMMVRIPFDPPMLDIVLSMVLLVAGFIGTT
                     WLAGKIYRTGILMYGKKITYKELFKWLSYK"
BASE COUNT          795 a          439 c          523 g          720 t
ORIGIN      
        1 atcattggca agcacaataa ctgcacatag cagtaatccg tttgaagaaa tgatcagacg
       61 ttttgatgtt gcagcaaaaa ttttaaacct cgaagccgac atttacgatg taatgaaata
      121 tccggctaaa aacatagagg ttaacctacc cattatgatg gataatggat taataaaagt
      181 ttttaacggc tatcgcgtta tccactcaac agcattaggc cccagcaaag gaggcattcg
      241 ctatagcatg gatgtgaacg aagatgaggt aaaagcctta gccgcttgga tgacctggaa
      301 atgcgcaatt gccgatattc cttatggtgg tgcaaagggt ggcattaaat gcgaccccag
      361 taaaatgagt gttggcgaaa ttgagcgcct aacccgtgct tacaccaaag ccatgagcga
      421 tatttttggt gttgataaag atatacctgc acccgacatg aatacaggcc cacgagaaat
      481 ggcctggata gtggatgaat ttagcaacct caaaggtggc tttacacccg gtattgttac
      541 cggtaaacct ttgcatttag gtggttcatt aggtagggta gaggcaaccg gtcgtggagt
      601 taatacttcg gctatttgcg ctatgaataa aatgaaactt aatcctaaaa aatcaacttt
      661 agccgtacaa ggttttggta atgtaggttc gattagtgca cgttttttag ttgaacaagg
      721 attaaagtta gttgccatca gcgattatac cggtgcttat tacaacccaa aaggtattga
      781 tttggcaaaa gcattaactt accgcaacag taacaaaggc aacctcgaag gttttaaagg
      841 cggaagcaaa ttaaccaacg atgatttgtt gtgtttaaaa gtagatgtgt tggcaccctg
      901 tgctttagaa aaccaaatta ccaaagacaa tgctgccaac gtacaagcta agcttattgt
      961 tgaaggtgca aacggaccta ctacttcggc tgccgatgat attttaaaca aaaaaggagt
     1021 aattgtagtg cccgatattt tagccaatgg tggcggtgtt accgtttcat atttcgagtg
     1081 ggtacaaaac cgaaccggtt actattatag cgaagaggag attaataaac gcgccaataa
     1141 ctggatgaca aaagcttttg aaaatgtttg gagtgtagct caaaagcata aaacatcatt
     1201 gcgtattgct gcttatgtat atgctatgaa taaagtagct acagccatta aaagccgtgg
     1261 ctcttattaa taaattaaac agtttaaaag gtcagtttgt ttgctataca aactgacctt
     1321 ttatttttat ttcagaatta taatataaac tttttaagta atgactattc ataaggaagg
     1381 ttatcggact ataacttata cggcactttt ttgcttactg gtaaatgtga ttgtatttta
     1441 ttttttccca atgcacgact ttttgcgtat agcaagtttg gtggtaacag tagcattgtt
     1501 tttaattata ctccagtttt tcaggctacc tgcacgtaaa gtaaaacgta acgattcgtt
     1561 ggtgttagcg ccttgcgatg gtaaggtagt ggttattgaa gaagtttttg aagccgagta
     1621 ttatcaagcc aagcgattac aggttagtat ttttatgtcg cctataaatg tgcatgccaa
     1681 ttggtacccc attagcggta ttgtaaaata tgtaaagtat catacaggca aatacttagt
     1741 agcctgggat cctaaatcat caaccgaaaa tgagcggagt actattgttg ttgaaaaaga
     1801 aaacaaagtt tcggttttgt tcaggcaagt agccggtgct ttagccaagc gcattatata
     1861 ttatccgcac gagggcgata tggtaaaaca aggcgctgag tttggcttta taaaatttgg
     1921 ttcaagggtt gatatttacc tgccgctaaa cgccaaggtt aaggttaaaa taggcgacaa
     1981 atcaacaggc ggtattaccg aagttgccga aattgagtaa ggtattcaac tttatttttc
     2041 atccactacc atttatttgt ttagaggcat gcgcttttct acttatacga aagccatttg
     2101 aacaactctt tgtacgttat ttttttgcca tacatcaaga tgccggtgcg ataaatttta
     2161 cctgccagcc aggttgtacc aataaaaccg gcaaccagta aaaccatcga caaaactata
     2221 tctaacatcg gtgggtcaaa aggtatacga accatcataa cgatgggtga tgttaagggt
     2281 ataatcgaaa accaaaatcc catactgcta tcggggtttt gcataacaat ggtacttgcc
     2341 acgtaagcaa aaattaatgg tatggtaatg ggtatcataa attgctggac atcggtttcg
     2401 ctatcaaccg ctgcgcctat ggctgcaaac aaggcactgt ataataagta accaccaaca
     2461 aaataaaata aaataaa
//
LOCUS       DAOMAA010000234         2475 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_37721_length_2475_cov_2.347521, whole genome shotgun sequence.
ACCESSION   DAOMAA010000234 DAOMAA010000000
VERSION     DAOMAA010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37721_length_2475_cov_2.347521"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="PLO59_04020"
     CDS             complement(<1..568)
                     /locus_tag="PLO59_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQV00300.1"
                     /translation="MYIEQLYTNCLAEAAYYIESEGEAIIIDPIRETTPYLQKLTERN
                     AQLKYVFETHFHADFVSGHIDLANKTGAVIVYGPNAQTDYKTYSATDGEIFKLGNITF
                     EVLHTPGHTLESTCYLLRDKNQKPYCVFTGDTLFVGDVGRPDLLDGVMSKDDLAAMMY
                     QSLNQKLKVLPDDVIVYPAHGPGSACGKN"
     gene            complement(712..>2475)
                     /locus_tag="PLO59_04025"
     CDS             complement(712..>2475)
                     /locus_tag="PLO59_04025"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HQV00301.1"
                     /translation="AEYKGNTGVNLEDIGRSIALDGSGNVYVCGSAGVLNRSGEFMVL
                     KYNNNGVLQYNKLLSGTNTNTYDEAYDIAVDNAGNAYVGGYYYNGAPEYYAAVLVKLT
                     PAGNVDFTKQYNGQTAQSYTEYFNRVMLKGNNVYATGTQKRDANVFDMDVFTVKVSTT
                     GAQQWIKIYDNTNNTNTYNYSQDLWVDNSNNVYALAVSNDTLWTLLKYDSVGTQLWIY
                     NHDRHHGQSANLITGYSYRPQLAADNLGGVYIGGTTYHGYNGYNNGFMRFCENPPVPA
                     NIASGPLTFCAGGSVTLNAASGIGYLWSNGATTQSIVANTSGNYRVTVTYNNGCSNLS
                     ANRNVTVNPLPTPTITGNGPLTFCEGSNVTLTTATASNYLWSNGATTKSIVVDTSGTF
                     TVTVTNSNGCTGTSASVATIDNPLPNATATAQSPTTICSTDSVKLVANGGNNITYQWR
                     RGNNNVAGATAKTFYAKLQGNYTVRVTSTNTGCSRTSAAVNVVVNCKMFAADIAQPDL
                     QAYPNPTTGVIHLTGTQMANEVALNVYDAAGKSILNKTLHSENGNLYYDIDLTQYPTG
                     IYRFMLISNNQIIHKQAFKID"
BASE COUNT          676 a          494 c          501 g          804 t
ORIGIN      
        1 tatttttgcc gcaagccgaa cccggaccat gtgccggata tactataacg tcatcaggca
       61 atacttttaa tttttgattt aaagattgat acatcattgc tgccaaatca tctttactca
      121 taacaccatc caataaatcg gggcggccaa catctcctac aaacaaggta tcgcctgtaa
      181 atacacaata tggtttttgg tttttatcgc gcaataaata acaagttgac tctaatgtat
      241 ggccgggggt gtgcaatact tcaaacgtaa tattgccgag tttaaaaatt tcgccatctg
      301 tagcggaata tgttttataa tcggtttgtg cattaggtcc gtaaacaatt acagctccgg
      361 ttttgtttgc caagtcaata tgccccgaaa caaaatcggc atgaaaatgt gtttcgaaaa
      421 catattttag ttgagcattc cgctcagtaa gtttttgtaa gtatggtgtt gtttcgcgta
      481 tggggtcaat aataattgcc tcgccttcgg attcaatata ataagctgct tcggcaaggc
      541 aattggtata taactgttcg atgtacatat tttaaagttt tagccggcaa atatacaata
      601 aagctatata gttgtttgtg caactattta aggatttagt ctggcttggc aggtattaaa
      661 agcggaaagg ccttgctaat gcaaggcctt tccgctttta aacaaatttg tttaatcaat
      721 tttaaatgct tgtttgtgta taatctggtt gttgctgatt aacatgaaac gatatatacc
      781 ggtagggtat tgcgttaagt caatatcata atacaggttt ccattttcac tatgcaaggt
      841 tttatttaaa atacttttac ctgcagcatc atatacgttt agtgctactt cattggccat
      901 ttgtgtgccg gttaaatgta tgacacccgt agtagggtta gggtatgctt gtaaatcggg
      961 ttgcgcaatg tcggctgcaa acattttgca gtttactact acattaacag cagccgaagt
     1021 acgtgaacaa ccggtatttg tactggtaac ccttaccgta taattaccct gtaattttgc
     1081 ataaaaagtt ttagcggttg caccagctac gttgttattg cctctgcgcc attgataggt
     1141 aatattatta ccgccattgg ctaccagctt aacactatcg gtactgcaaa tggtggttgg
     1201 cgattgtgca gtagcagttg cattgggtaa cggattatca atggtggcaa cagaagccga
     1261 tgtgcctgta catccattac tgtttgtaac tgttactgta aaggtaccac tggtatcaac
     1321 tacaatgctt tttgtagtgg caccgttgct ccataaataa ttgctggctg ttgctgttgt
     1381 tagggtaaca ttactgcctt cgcaaaaagt taacgggcca ttaccggtaa tggttggcgt
     1441 tggcaatggg ttaacggtta catttctgtt ggccgataaa ttgctgcaac cgttattata
     1501 agtaacagtt actctatagt tgccactggt gttagcaact atgctttgcg tagtggctcc
     1561 attactccat aaataaccaa tacctgaagc tgcattaagt gtaactgatc ccccggcaca
     1621 aaatgtaagc ggacccgaag ctatgttggc aggtaccggt gggttttcgc agaaacgcat
     1681 aaagccatta ttatagccgt tatagccatg atatgtagtg ccgccaatat atactccgcc
     1741 taaattatcg gcagctaact gagggcgata actatagcct gtaattaaat tggcgctttg
     1801 gccgtgatgc ctgtcgtgat tatatatcca aagttgtgtg cctacactgt cgtattttag
     1861 gagtgtccat aaagtatcat tcgacacggc taaagcatat acattattcg aattatcaac
     1921 ccataaatcc tgactgtagt tgtaggtgtt ggtattgttg gtgttatcgt atattttaat
     1981 ccattgttgt gcgcctgttg tacttacttt tactgtaaac acatccatat caaacacgtt
     2041 ggcgtcacgt ttctgtgtgc ctgttgcata cacattgttg cctttcagca ttacacgatt
     2101 aaaatattcg gtatatgact gtgccgtttg tccattgtat tgtttggtaa aatcaacatt
     2161 tccggccggg gttaacttta ccaatacagc ggcataatac tcgggtgctc cattatagta
     2221 atagccacct acataggcgt taccggcatt atcaacggca atatcataag cttcgtcata
     2281 ggtgttggtg ttggttccgc taagtaattt attatactgc aacaccccat tgttattgta
     2341 tttaagcacc ataaactcgc cactgcgatt taatacacct gccgagccac atacataaac
     2401 attgccactg ccatctaatg caattgatct gccaatatcc tccagattaa cacctgtatt
     2461 gcctttatac tcggc
//
LOCUS       DAOMAA010000235         2474 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_37740_length_2474_cov_2.662671, whole genome shotgun sequence.
ACCESSION   DAOMAA010000235 DAOMAA010000000
VERSION     DAOMAA010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37740_length_2474_cov_2.662671"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="PLO59_04030"
     CDS             complement(<1..900)
                     /locus_tag="PLO59_04030"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HQV00302.1"
                     /translation="MKTRVFIAALVITLLINGNLKAQSLYFPTQNNWDTIAPASLGWC
                     QPYIDTLYNYLEQNNSKAFIVLKDGKLVLEKYFGTFTADSVWYWASAGKSLTAFMVGI
                     AQQEGYLNITDTTSKYLGAGFTACTAAQEDAITIKHQLSMTTGLDDGVPDNYCTLDTC
                     LIYKAQAGSRWAYHNAPYTLLDSVIETATGQSLNSYTASKLGLTGISGLFLPSGFNNV
                     FYSKPQAMARFGLLMNANGVWNGNAVLSDTNYFNQMINTSQNLNQSYGYLWWLNGKAS
                     FMLPQLQFTFQGNLFSHAPADMYA"
     gene            complement(937..1572)
                     /gene="can"
                     /locus_tag="PLO59_04035"
     CDS             complement(937..1572)
                     /gene="can"
                     /locus_tag="PLO59_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HQV00303.1"
                     /translation="MEKSYQKLLDSNKQWVAKQLELDPTYFETLAKGQSPEYLWIGCS
                     DSRVPANEVTGTRPGEIFVHRNIANMVVHTDMNMLSVLSYAVEVLKVKHIIVCGHYGC
                     GGVIAAMGNKQFGLIDNWLRHLKDVYRMHSDELEAIEDIKARENRFVELNVQEQVTDL
                     CKTSIVQNAWRNNQPLHVHGWVYDIADGIIQDLDVSYRQASDLEKIYQFKL"
     gene            complement(1577..>2474)
                     /locus_tag="PLO59_04040"
     CDS             complement(1577..>2474)
                     /locus_tag="PLO59_04040"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HQV00304.1"
                     /translation="HEFFSFFKMPDFEGFLESKVWVTGVMIALIASLETMLSIEAIDN
                     IDPDRNVTNTNRELVAQGIGNTLSGLIGGLPITSVIVRSSANVNAGAKTRLSTMFHGF
                     LLLVCVVTIPFILNKIPLSALAAVLLITGYKLCKISIFKDMIKNGKYQYIPFFMTVTV
                     ILGIDLFGLVPAVRGEGLLIGVIVGFLSASIAILHGNFKNSYYFHEEEHKEGDIITIK
                     LSQEVSFLNKASIRESLDHLPKNSTVIINASETYYIDFDVLEFIKEFRDIKAPLKQIK
                     LTLVGFKEEYQINNHYYISSGH"
BASE COUNT          795 a          490 c          398 g          791 t
ORIGIN      
        1 tgcatacata tcagcaggcg catgcgaaaa aagatttccc tgaaaagtaa attgcaattg
       61 aggtaacata aaactggctt tgccatttaa ccaccacaaa taaccatatg attgatttag
      121 gttttgagat gtgttaatca tttggttgaa gtaattggta tcggataata cggcattacc
      181 attccaaaca ccatttgcat tcatcaacaa accaaagcgc gccatagcct gcggcttact
      241 ataaaagaca ttattaaaac cactgggtaa aaacaaaccg cttatacctg ttaaaccaag
      301 tttagatgct gtgtaagaat ttagtgattg accggtagcg gtttctatta cactatccaa
      361 caatgtatat ggtgcattat gataggccca cctgctaccg gcctgtgctt tataaattaa
      421 acaagtatct aaggtgcagt aattgtcggg cacaccatca tctaaaccgg tagtcatact
      481 taactgatgc tttattgtta ttgcatcttc ttgtgcagca gtacaagcag taaagccggc
      541 acctaaatat tttgatgttg tatcggtaat attcagatag ccctcttgtt gcgctatacc
      601 caccataaat gctgttaacg atttgcctgc cgaagcccaa taccatacac tatcagccgt
      661 aaaagtgcca aaatattttt ccaacaccag tttaccatct tttaatacaa taaaagcttt
      721 ggagttattt tgttccaaat aattgtaaag cgtatcaatg taaggttggc accagcccaa
      781 tgaagctggt gctatggtat cccagttgtt ttgagttgga aagtataaac tttgcgcctt
      841 taaatttcca tttattaata atgtaataac cagcgcagca ataaaaactc gtgttttcaa
      901 tcgaagtaaa tttctgactt tgacaccaat tccttattat agtttaaact gataaatctt
      961 ttctaaatca cttgcctgac ggtagctaac atctaagtcc tgaataatac cgtctgcaat
     1021 atcatatacc catccatgca catgtaaagg ttggttgtta cgccaggcat tttgcacaat
     1081 agaagtttta cataaatcgg taacctgttc ttgtacattc aattcaacaa atcggttttc
     1141 gcgggccttt atatcctcaa ttgcctccaa ttcatcgcta tgcattctgt aaacatcttt
     1201 taaatggcgt aaccaattat ctattaagcc aaattgttta ttacccattg ccgcaattac
     1261 accaccacag ccataatgac cacaaacaat aatgtgtttt acttttaaaa cttctacggc
     1321 ataagataat acgcttaaca tattcatgtc ggtatgtaca accatatttg caatgttgcg
     1381 atgtacaaat atttcgccag gccgtgtgcc tgtaacttcg ttagcgggca cacggctatc
     1441 actgcaacct atccataaat attcaggact ttgtcctttg gctaaggttt caaaataagt
     1501 tgggtcaagt tccaattgtt tggctaccca ttgtttatta ctgtctaata atttttgata
     1561 cgatttttcc ataatattaa tgtcctgatg aaatgtaata atgattgtta atttgatatt
     1621 cttctttaaa acctacaagg gttaatttaa tttgctttaa gggtgctttt atgtctctaa
     1681 actccttaat aaactccaac acatcaaagt ctatataata agtttctgat gcatttataa
     1741 taacggtact gtttttaggc aaatgatcta atgattcgcg aatactcgct ttgtttaaaa
     1801 aagatacttc ttgcgaaagt ttgatagtga ttatatcgcc ttctttatgt tcttcttcat
     1861 gaaagtagta cgagttttta aaattaccat gtaaaatggc aatactggcc gaaagaaatc
     1921 ccacaataac gccaattagt aaaccttcgc cacgtacagc agggaccaag ccaaataaat
     1981 caattcctaa aattactgtt accgtcataa agaacggaat atattggtac ttcccatttt
     2041 taatcatgtc tttaaaaatg gaaattttac aaagtttgta acctgtaata agtaaaactg
     2101 cagccaatgc cgacaacgga attttattaa gtataaatgg gatggttact acacatacca
     2161 gcaataagaa tccatgaaac atggttgata aacgggtttt agcacctgca tttacattgg
     2221 cactactgcg tacaataaca cttgttatag gcaagccacc aatcaaaccg gatagggtgt
     2281 taccaattcc ttgggcaacc aattctctgt ttgtattggt tacatttcta tcggggtcaa
     2341 tattatcaat ggcttcaata ctaagcatgg tttcgagcga agcaattaat gcaatcatta
     2401 cacctgtaac ccaaacttta ctttctaaaa agccttcaaa atcgggcatt ttaaaaaaac
     2461 tgaaaaactc atgt
//
LOCUS       DAOMAA010000236         2474 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_37745_length_2474_cov_2.446879, whole genome shotgun sequence.
ACCESSION   DAOMAA010000236 DAOMAA010000000
VERSION     DAOMAA010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37745_length_2474_cov_2.446879"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1462
                     /locus_tag="PLO59_04045"
     CDS             <1..1462
                     /locus_tag="PLO59_04045"
                     /inference="COORDINATES: protein motif:HMM:NF024574.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQV00305.1"
                     /translation="ALLFSLWVLYLLIPVANQSKSKTKITNEKKGVKNVTMPVQSTKP
                     QQTTCVLKVYLYAPILFFLALLSKEVAIVFLALIPIALWLFANKNVKTIAKQTAPLVI
                     ITIGYLALRSYYAGTVGDRVTTDIMDDSYLYATLMQKWATITQVQLRYLGLLIFPYPL
                     SFDYSFNQIPLVNWSNVWVIISVIIHIALLLFAILFIKRQKLLSFGILVYFAGFVLVS
                     NLFFNIGTSMAERFAYMPSLGFCLALAFLIFKVLKLGEMPSNKFSTSYIAIVIVISLI
                     AGAQTIARNADWKNNFSLYTADINKVPNSARAQLYYGIENIGPYQQTGDVTYINNAIT
                     AITRATLINPQFHYAWHNLGVAYQTINKWPESVTCYQKVLQLQPGNEQAYYGLGLAYG
                     KGLNQPNKAIPYFSTLVDSLKLTRPDYFEGLGLCYATQGQWQQALQSFKRGISFNPAS
                     GKLYYDVAITYANAGYADSSKIYFDQAFMLDPSLKR"
     gene            1547..>2474
                     /locus_tag="PLO59_04050"
     CDS             1547..>2474
                     /locus_tag="PLO59_04050"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HQV00306.1"
                     /translation="MNNEQGFTQFKLNKQIFNAIAETGFTEPTAIQRKAITPILAGQE
                     VIGIAQTGTGKTAAYLLPLIKMLGYAQGVHPRALIVIPTRELAQQVANNFTLFAKYTD
                     LRCTVAIGATGSKKQIEAIEAGIDILIATPGRLLELYSEGHLILKKIEYFVLDEAERL
                     MDISFMRQLYALLEVLPRKRKHYLFSATMSDLVKKIAADFVAFPTVIEIEPEQKTAKT
                     ITQLAYALPNLKTKLNLLTYLLKQTTEVGVIIKKLIVFCRSKETANNLYKYFTRINGA
                     DYATVVHGNKAQQSRLNAVNNFTHQAITMLIAT"
BASE COUNT          779 a          471 c          456 g          768 t
ORIGIN      
        1 tgcactcctg ttttcattat gggtacttta cttattgata ccagtagcta atcaaagtaa
       61 aagcaaaaca aaaattacga atgaaaagaa gggtgtaaaa aatgtaacca tgcctgtgca
      121 gtcaacaaaa ccacaacaaa ccacatgcgt tttgaaagtg tatttatatg cacccatttt
      181 attttttttg gcattacttt caaaagaagt agccattgtt tttttagcat taatacctat
      241 agcattgtgg ttgtttgcta ataaaaatgt aaaaactatt gcaaagcaaa cagcaccttt
      301 agtaataata acgataggct accttgcttt acgcagttat tatgccggta ccgtaggcga
      361 tcgtgttact accgatatca tggacgactc ctatctctat gcaaccctta tgcaaaaatg
      421 ggcaaccatt acacaagttc aattgcgtta tttgggttta cttattttcc catatccatt
      481 aagcttcgat tattcattca atcaaatacc cttagtaaat tggagtaacg tatgggttat
      541 aattagtgta atcatacaca tagcactcct gttatttgcc atactattta ttaaaagaca
      601 aaagctgttg agctttggca tacttgtata ttttgcaggc tttgtattgg taagcaacct
      661 gttttttaat attggtacca gtatggccga gcgttttgca tacatgccat ctttaggttt
      721 ttgtttagca ctggcatttt taattttcaa agttttgaag ttaggtgaaa tgccctccaa
      781 taaattttca acatcgtata ttgctatagt tattgtgata agcttgatag ctggcgcgca
      841 aaccattgcg cgtaatgccg attggaaaaa caatttttca ctttacacgg ccgatataaa
      901 taaggtgcct aacagtgcac gggcacaatt atactacggt atcgaaaaca ttgggcccta
      961 ccagcaaaca ggcgatgtaa cctacatcaa caatgccatt acagcaatta ccagggctac
     1021 gttaattaat ccgcaatttc attatgcctg gcataactta ggtgttgctt atcaaacaat
     1081 caataaatgg cctgagtctg ttacctgtta tcaaaaagta ctgcaactgc aaccgggtaa
     1141 cgaacaagct tactatggtt taggtttagc ctatggcaag ggactgaatc aaccaaacaa
     1201 agccattcct tatttcagca cattggttga cagcctgaaa cttacgcgcc ccgattattt
     1261 tgaaggcctt ggattatgtt atgctacgca aggacaatgg caacaagcat tacaaagttt
     1321 taaacgcggt atttcattca atccggcttc gggtaaattg tattacgatg tagctattac
     1381 ttatgccaat gcaggatatg ctgatagcag taaaatttat tttgatcagg catttatgtt
     1441 agacccttca ctaaagcggt aattacttgt tacagtttag tttaatttaa tttgggcttt
     1501 tactttttta aaattactag gcagctatca ttaaatttgc aagcttatga ataacgagca
     1561 aggctttacg cagtttaaac taaacaagca aatttttaat gcaattgccg aaaccggttt
     1621 tactgagcct acagctattc agcgaaaggc cataacgccc atacttgctg ggcaagaagt
     1681 tattggtatt gcacaaaccg gtaccggtaa aacagctgct tatttattac cactaattaa
     1741 aatgttgggt tatgcacaag gtgtgcatcc gcgtgcatta attgttatac ccacacgcga
     1801 acttgcacaa caggtagcaa acaattttac cttgtttgcc aaatacactg acttgcgttg
     1861 caccgttgca ataggcgcca ccggtagtaa gaaacaaatt gaagccattg aagccggcat
     1921 tgatattttg attgcaacgc cagggcgttt gttagagttg tatagcgaag gacatcttat
     1981 tctaaaaaaa attgaatact ttgttttaga tgaagccgag cggttgatgg acataagttt
     2041 tatgcggcag ctatatgctt tgttagaggt gttgccccgc aagcgtaagc attacttatt
     2101 tagcgctacc atgagcgatt tggttaaaaa aattgcagcc gattttgtgg catttccaac
     2161 tgtaatagaa attgaacccg aacaaaaaac agctaaaacc attacgcaac ttgcttatgc
     2221 attgcctaat ttaaaaacca aacttaatct gttaacctat ctgttaaagc aaacaactga
     2281 ggttggtgta attataaaaa agctcattgt tttttgccgt agtaaagaaa cagctaacaa
     2341 cttgtataaa tattttacac gtattaatgg ggctgactat gccacagtgg tgcatggtaa
     2401 taaagcacag caaagtcgcc tgaatgccgt aaataatttt acacatcaag ccattaccat
     2461 gcttattgct accg
//
LOCUS       DAOMAA010000237         2472 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_37800_length_2472_cov_2.669425, whole genome shotgun sequence.
ACCESSION   DAOMAA010000237 DAOMAA010000000
VERSION     DAOMAA010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37800_length_2472_cov_2.669425"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="PLO59_04055"
     CDS             <1..559
                     /locus_tag="PLO59_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQV00307.1"
                     /translation="HNVLFINDEIQTGIARTGKMLAADYENVHADILILGKALSGGMY
                     PVSAVLCNNEIMLCIKPGQHGSTYGGNPLACHVAMAAMQVVLEENLANNAMRMGVLLR
                     SELQKINSPMITAIRGKGLLNAIDIAPLNGKTAWDVCVKLSDNGLLAKPTHGHIIRFA
                     PPLIITEDQMLDCVEIIRKTILSFG"
     gene            632..1669
                     /locus_tag="PLO59_04060"
     CDS             632..1669
                     /locus_tag="PLO59_04060"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HQV00308.1"
                     /translation="MSTKYLLLSIDGGGLRGIVPLQFLKYIENRTGKRIQNCVHFIAG
                     TSTGGLIACALAVQKDNQPKFGLSDIEKIYIEHGKDIFPEANAIGKFFRKLKSVVDPT
                     FSAKGLSAVLKSNFGSDTMLNCTIPILVPAFDLKNNFAVMFKYRHAKKDPALNVSLYD
                     ICRSTSAGPTYLPSHKFECMDENTGRIIERVCIDGGVYINNPGVAAYVEFRKYFKDFY
                     SKPNDEHNENNWFMLSLGTGRYSDSIDKSKTMGQIEWATKISDVMMQGVNMVTSYECN
                     ELFKTGNFLRLQIEIDDEKHSDMADSSDATRNYLIQKANTYLADPKVQYQIEAFLYNA
                     GLIETPVGPPV"
     gene            complement(1726..2244)
                     /locus_tag="PLO59_04065"
     CDS             complement(1726..2244)
                     /locus_tag="PLO59_04065"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HQV00309.1"
                     /translation="MKPYIKFGLLAGLFSIIWTLVMYVFELDKSSFSKALGWISIIAS
                     LAFIVLAVKESKSVAGGYINFGDAFKDGFLVMLISGIIGVGYFYLHVSFVDDGFVEHI
                     VENTRLEMSKQQDLTEEQMEQAWKYTEMFLTPGVMAFFGLLAHLVTGTIGALIIAAIM
                     RKEPPVFNEVTD"
BASE COUNT          845 a          422 c          430 g          775 t
ORIGIN      
        1 acataacgtg ctttttatta atgatgaaat tcaaacaggt atagcacgta caggtaaaat
       61 gcttgcagcc gattatgaaa acgtacatgc tgatatctta attttaggca aagcgctatc
      121 aggagggatg taccctgtaa gtgcagtgct ttgcaataat gaaataatgc tttgtattaa
      181 accaggccaa cacggcagta cttatggtgg taatccattg gcctgccatg ttgcgatggc
      241 agctatgcaa gttgtattgg aagaaaactt agcaaataat gctatgcgaa tgggtgtatt
      301 gcttcgtagt gaattacaaa aaataaactc gcccatgatt accgccatac gtggcaaagg
      361 attacttaat gctatagata ttgcgccttt aaatggtaaa actgcttggg atgtatgtgt
      421 aaaattaagt gataatggcc ttttagcaaa acctacacat ggccatatta tccgttttgc
      481 acctccatta ataataaccg aagatcaaat gttggattgt gttgaaataa ttcgaaaaac
      541 aattttatcg ttcgggtaac caaaagcatt ttgttgatat acttttaggg tattagaaat
      601 ttgttatttc ttattactaa acctaaaagt tatgtcaacc aagtatttat tgctaagtat
      661 tgatggtggt ggccttagag gcattgtacc gctacaattt ttaaagtaca tcgaaaatcg
      721 aaccggtaaa cggattcaaa actgtgttca ttttattgcc ggtacatcaa ccggtggact
      781 cattgcatgc gctttagcgg tgcaaaagga taatcaacca aaattcggct tatccgatat
      841 cgaaaaaatt tatatcgagc atggaaaaga tatttttccg gaagccaatg ctattggcaa
      901 attttttcga aaattaaaat cagtagttga tccaaccttt agtgccaagg gattaagcgc
      961 tgttttaaaa agtaattttg gtagcgatac catgcttaat tgtacaatcc caatcttagt
     1021 acctgcattc gatttaaaaa acaattttgc tgttatgttt aaatacaggc atgctaaaaa
     1081 agatccggca cttaacgttt cgctatatga tatatgtcgt tcaacatctg ccgggccaac
     1141 ctatttgcct tctcataaat ttgaatgtat ggacgaaaac acaggtcgca ttatcgaacg
     1201 tgtttgtatt gatggtggtg tttatattaa taatcccggt gtggctgctt atgtcgagtt
     1261 tagaaaatac tttaaagatt tttacagcaa accaaatgat gagcataatg aaaacaattg
     1321 gttcatgtta agtttaggta ctggccgata cagcgacagc attgataaat cgaaaacaat
     1381 gggtcaaatt gaatgggcca ccaaaattag tgatgtaatg atgcaaggtg taaacatggt
     1441 tacatcttat gaatgtaatg agctttttaa aaccggtaac tttttaagat tacaaattga
     1501 aatagatgat gagaaacata gtgatatggc cgactcgagc gatgctacca gaaattatct
     1561 tatacaaaaa gcaaatacat atttagctga ccccaaagta caatatcaaa ttgaagcatt
     1621 cctttataat gcaggactga tagaaacacc agttggtcca ccagtgtaaa ttaagtagtt
     1681 tagaaaaaga ggctgtctca aaaataggca gcctcttttt tttttttaat cagtaacctc
     1741 gttaaaaaca ggaggttctt ttctcataat tgctgcaata attaaagcac caattgtacc
     1801 ggttactaaa tgggctaata aaccgaaaaa cgccataacg cccggtgtta aaaacatttc
     1861 agtgtatttc caggcttgct ccatttgttc ttcggttaaa tcttgttgtt tgctcatttc
     1921 taatcgcgta ttttcaacta tatgttcaac aaaaccatca tctacaaaac ttacatgtaa
     1981 ataaaagtat ccaacgccaa taatgccact tattaacatt accaaaaagc catccttaaa
     2041 tgcatcacca aaattaatat atcctccggc aacacttttc gattctttaa ccgctaatac
     2101 tataaatgca agcgaagcaa ttatcgaaat ccaacctaat gcttttgaaa aagaactttt
     2161 atctaattca aatacataca taactaaagt ccagatgatg gaaaaaaggc ctgctaataa
     2221 tccaaatttt atataaggtt tcatgtaagg tttgttatag tttgctatat gattctgttt
     2281 ctttaaaaaa gttacgcaat aaagtaaatt atcaggagca ttataggctg ctatggttta
     2341 aaatattagc atcgcacatt atttaataac ctatgttata tcaaaccaaa tatcaggctt
     2401 ttataaaatg tgtactgttt tatttaatac catacactac aaatgcacag gtacaatcgc
     2461 atcaaatttt ag
//
LOCUS       DAOMAA010000238         2471 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_37832_length_2471_cov_2.774834, whole genome shotgun sequence.
ACCESSION   DAOMAA010000238 DAOMAA010000000
VERSION     DAOMAA010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37832_length_2471_cov_2.774834"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1703
                     /locus_tag="PLO59_04070"
     CDS             <1..1703
                     /locus_tag="PLO59_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00310.1"
                     /translation="EWGETIDYLSSDTPSEPRNFCVVDNETIVTVGKIFYTGNWDLYL
                     TADALCKVYYPRYIWVNNVYNAALVNDSVNILNPDSSTLYQSRLNNLVNYLATLQYSG
                     VVITHLEDIFIDSASITLQSQLHLVNAVLHKRGIPIILNQFRANEIKRKPTVSKNNHL
                     DYLNQSNLYNQTAPVLSRFDGLELDYEFWQKEYGKANSWDSAVVPPVFIPPFAHIYTG
                     YQNYLTIAHAFKTAKADTANHYKLLAQWIGLVFDCDTFDVNANNTAPEAADNAALMYT
                     FDSLQFDRNVLSFFLDDKLNDSSGYAIFKDPLTFLQRSAIGVKINHYFARLYWLGQNN
                     YPTNVVPQFHAGTGVNGNPNLELYLSGSGTWAGTPHANNIPHYLNEVEQEFASQYFDP
                     NIFASQNQGPTILITNTTWGTTTIMQGFEWFKYQIGPLDFDSLPARHNDKTKSMYWAC
                     ASTQRLHSQTPVPVALNFQTTHNVLLRITPKPVTDIATLYIDNFTCDNPQAVTLQCYD
                     AIGKPLNNVLVINHADATEFGIQLQLVKKIDYSGLLFIKVNYNQTCLGTLKTISISNK
                     "
     gene            1708..>2471
                     /locus_tag="PLO59_04075"
     CDS             1708..>2471
                     /locus_tag="PLO59_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00311.1"
                     /translation="MKKIILALLIACNCMAQPQITWQKYFYTSTGNNVMNYIVEMEDS
                     GFVTGTVMPQFNPSCRIIRTDKSGDTLWVKKENWGGNMYKAPTYGYICSGYKSYGQLV
                     RLSFNFDTIWVKDYLQSPGFDIATCNDGGFLLITHNFVSKTDSLGNILWSKNIGGSTY
                     NALSCKNGDILVMGKFSNGPDQYFAKLYDSTGTLLWNRTYGNWYISWSQSSFGGILDA
                     VELPDGNFMAACIAQWGITNAGGDWDLMKIERFTGDT"
BASE COUNT          773 a          492 c          461 g          745 t
ORIGIN      
        1 gcgaatgggg cgaaaccatt gactacctaa gcagcgatac cccatcagaa ccacgtaact
       61 tttgtgtagt tgataacgaa accattgtta ccgttggtaa aatattttat accggcaact
      121 gggatttata ccttactgcc gatgcacttt gcaaagtata ttacccacgt tatatttggg
      181 taaacaatgt gtataatgct gcacttgtta acgacagtgt aaatatttta aaccccgata
      241 gcagtaccct ataccaaagc aggttaaaca atttggtaaa ttacttggcc acattgcaat
      301 attcaggagt ggttattaca catctcgaag atatttttat tgactcggcc agcataaccc
      361 tgcaatccca attacatttg gtaaatgctg tattgcataa acggggcata ccgataatac
      421 ttaatcagtt tagagccaat gaaattaagc gcaaaccaac agtaagcaaa aataatcatt
      481 tggattattt aaaccagagt aacctgtata accaaactgc ccctgtttta tcccgttttg
      541 atggcttgga gttagactat gagttttggc aaaaagaata tggcaaagcc aacagttggg
      601 atagcgctgt tgtgccaccg gtatttattc caccctttgc acatatttat acgggctatc
      661 aaaattactt aaccattgcc catgccttta aaacagccaa agccgatact gcaaaccatt
      721 ataaattatt ggcacaatgg attggattgg tttttgactg cgatactttt gatgttaatg
      781 ctaacaatac agcacccgaa gctgcagaca atgcagcttt aatgtacacc tttgatagct
      841 tgcagtttga tagaaatgta ctttcattct tcttagatga caaactcaac gattcttcag
      901 gctatgctat ttttaaagac cctttaactt tcctgcaacg atcggccatt ggcgttaaaa
      961 tcaaccatta ttttgctaga ttgtattggc ttggccaaaa caattatccc accaatgtag
     1021 tgccgcagtt tcatgcaggt acgggcgtaa acggcaatcc aaacttagaa ctttatcttt
     1081 caggttcagg tacctgggct ggtacgccac atgctaataa tatcccacat taccttaacg
     1141 aagtagagca agaatttgcc tcccaatatt ttgacccgaa tatttttgca tctcaaaacc
     1201 aaggacctac tattttaatt actaatacca cttggggcac aaccaccatt atgcaaggct
     1261 tcgaatggtt taaatatcaa ataggcccct tagattttga ttcattgccc gccaggcata
     1321 acgataaaac caaatcaatg tattgggctt gtgccagtac tcaacgtttg catagccaaa
     1381 cgcccgtacc ggttgcatta aattttcaaa ctactcataa tgtactttta cgtataaccc
     1441 ccaaaccggt tacggatatt gcaacgcttt atattgataa ctttacttgc gataatcccc
     1501 aagccgttac cttgcaatgt tacgatgcca taggcaaacc cttaaacaat gttttggtaa
     1561 taaaccatgc cgatgctacc gaatttggca ttcagttaca attggtcaaa aagattgact
     1621 atagtgggct gttgtttata aaggtaaact acaaccaaac ctgtttgggt actctaaaaa
     1681 caataagcat tagcaataaa taaagccatg aaaaaaatca ttttggcttt gctaattgct
     1741 tgcaattgca tggcccagcc ccaaattact tggcagaaat atttttatac ctcaaccgga
     1801 aacaatgtga tgaattacat tgttgaaatg gaagatagtg gttttgttac aggaactgtt
     1861 atgccacaat ttaaccctag ttgtagaata attagaaccg ataaatctgg ggatacacta
     1921 tgggttaaaa aagaaaattg gggtggaaat atgtataaag cacctacata tggttacata
     1981 tgttctggtt ataaaagtta tggtcaatta gttagactga gtttcaattt tgataccatt
     2041 tgggttaaag attatctaca atctcctggt tttgatatag ccacctgcaa cgatgggggc
     2101 tttttactta ttacacataa ttttgttagc aaaaccgaca gcttaggcaa tatactttgg
     2161 tcaaaaaata ttggcggtag cacatacaat gccctatcct gtaaaaatgg tgatattttg
     2221 gttatgggta aattcagtaa cggccccgac caatactttg ctaaacttta cgactcaacc
     2281 ggtactttgc tttggaaccg cacgtacggc aactggtata tatcttggtc acaaagtagt
     2341 ttcggaggca tattagatgc cgttgaattg cccgatggta attttatggc tgcatgtatt
     2401 gcgcaatggg gcataaccaa tgccggtggc gattgggatt taatgaagat agagcgtttt
     2461 acaggtgata c
//
LOCUS       DAOMAA010000239         2463 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_38083_length_2463_cov_2.330980, whole genome shotgun sequence.
ACCESSION   DAOMAA010000239 DAOMAA010000000
VERSION     DAOMAA010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38083_length_2463_cov_2.330980"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..1155
                     /locus_tag="PLO59_04080"
     CDS             52..1155
                     /locus_tag="PLO59_04080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQV00312.1"
                     /translation="MEVVFCTSGIFPESIGGIQRHSRLLIEALTAFNDIKLTVIHPHS
                     KLQFTHLPAVNEIQIPFNHHAGYYLYNCYLYSQLVYEQLKNKPNAIIYSQGLCIWHKA
                     HVFKNRLVFNPHGLEPFQALNKSDNYKLWPFRFVLKKVFKNCTYVVSLGGKLTKILEH
                     AGVKSDKIIELPNATIVPNEIPLKQKGSPFTFLFVGRFAYNKGIDVLLQAISLLDKTQ
                     AAQTNFILVGTGPLYQQLKSKYALSNVQYVGFADDNALASYYKQSQAFVLPTLFEGMP
                     TVVLEAMARALPVIVTDVGATAMLVDESNGYLIEKNNLNQLKEALIKMTSLSDEAYDK
                     LSKQSFEKVKTNFSWQAVAQQHYSLFTSLNSQL"
     gene            1158..>2463
                     /locus_tag="PLO59_04085"
     CDS             1158..>2463
                     /locus_tag="PLO59_04085"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HQV00313.1"
                     /translation="MLETLKRNYQLTIILFVWLLVGVYINVKPYPTTILIPLTVLLFK
                     AKNRYKEIFFGFLFMLAMSDSWSYSMFWTKDARNIYMLMLGVFYFFDRKNFNYPNKIF
                     LLFVPFLIWTCITNFRNDDMANAFQKTMSYTLMLIIIPAYFQKLIIEEGASFLRDLVF
                     FVAWLLLAGLILIPFNYDLVYLVERYRGVLGNPNGIGTFSFVFTTFFYILTQKFPHLF
                     SKQEKLFVYALITVSLLMCESRNGLMSILLFLFFIRFYKISAFAGFAVFVITLVSFQL
                     VSQNLTEIIKALGLSDVLRADSIESGSGRLVAWQFAWIEIQKNFFIGLGFDYDSQYFV
                     RNAKMLSWLGHNGGIHNVFLGLWMCFGLIGVILFYQALLRLFFRLANRSFIAIPLLYA
                     LLFSTSYEAWLMGSLNPFTVYLIMSLVLLDYDHQFSTSKKSLV"
BASE COUNT          742 a          425 c          436 g          860 t
ORIGIN      
        1 ttagcattga tgcaggcata aaaaccttgc ttcaaaacta aaggtacttt tatggaagta
       61 gttttttgta ccagcggaat ttttccggag tcaataggcg gcatacaacg ccactcacgt
      121 ttgcttattg aagcccttac tgcttttaat gatattaaac ttacggttat acatccccac
      181 agtaaattac agtttacaca tttacctgca gttaacgaaa ttcaaatacc tttcaatcat
      241 cacgctggat attatttgta caactgttat ttatactctc agttggttta tgaacaactt
      301 aaaaacaagc ccaatgcaat tatatattcg caaggacttt gcatctggca taaagcacat
      361 gtatttaaaa atcgattggt ttttaatccg cacggtttag agccttttca ggcattaaat
      421 aaatctgata attacaaact ttggccattc aggtttgtat taaaaaaggt gtttaaaaat
      481 tgcacctatg tagtttcgtt gggcggtaag ctcactaaaa tattagaaca tgccggtgtt
      541 aagtctgata aaattattga actcccaaat gctaccatcg ttccaaatga aataccttta
      601 aaacaaaaag gcagtccgtt tacatttttg tttgtaggta ggtttgcata caacaaaggc
      661 attgatgtat tgctccaagc catttcatta cttgataaaa cgcaagcggc acaaacaaat
      721 tttatattgg taggcaccgg cccattgtac caacagttaa aaagtaaata tgcactatca
      781 aatgttcaat acgttggttt tgcagacgat aatgcattag cttcatacta caaacaaagc
      841 caagcttttg tattacccac attatttgag ggtatgccta ccgtagtttt agaagctatg
      901 gcccgtgcat tacctgttat tgtaactgat gtaggtgcta cagctatgtt agttgatgaa
      961 agcaatgggt atttaattga aaaaaacaat ttaaatcaat taaaagaagc attaattaaa
     1021 atgacttcat taagtgatga agcttatgat aaacttagta agcaaagttt cgaaaaagta
     1081 aaaaccaatt tcagttggca agcagttgca caacaacact atagtttatt tacttcgctt
     1141 aacagccaat tataagcatg ttagaaacac tcaaacgcaa ctatcaacta accatcatat
     1201 tatttgtgtg gttgcttgtg ggtgtttata taaacgttaa gccatatcct acaactattt
     1261 taataccact aacggtattg ctttttaaag caaaaaacag gtataaagaa atatttttcg
     1321 gtttcctgtt tatgttagct atgagcgata gctggagtta cagcatgttt tggacaaaag
     1381 atgcgcgtaa catttatatg cttatgctag gcgtatttta ttttttcgat cgcaaaaatt
     1441 ttaattaccc caataaaata tttctgctgt ttgttccatt tttgatatgg acatgtatca
     1501 ccaattttcg aaacgatgat atggccaatg catttcaaaa aaccatgtct tacacattaa
     1561 tgctcataat tattccggct tattttcaaa aattaattat tgaagaaggc gcctcctttt
     1621 taagagactt ggtgtttttt gtagcctggt tgttgttagc cggtttaatt ctgatacctt
     1681 ttaattatga tttggtttac ttggtagaac ggtatagagg tgtgttaggc aacccaaatg
     1741 gtataggtac atttagcttt gtgtttacaa ccttctttta catattaact caaaaatttc
     1801 cgcacctgtt ttcgaaacaa gaaaagctgt ttgtatatgc attaattacc gtatcgttgc
     1861 ttatgtgcga aagccggaat ggcttaatgt ctattttgct ttttttattt tttatccgtt
     1921 tctacaaaat atcggcattt gcaggttttg cagtttttgt aattacgctt gtaagttttc
     1981 aattggtatc acaaaaccta accgaaataa ttaaagcact tggtttgtct gatgtacttc
     2041 gtgccgattc tatcgaaagt ggttcgggta ggttagtagc ctggcaattt gcctggattg
     2101 aaattcagaa aaacttcttt ataggtttag ggttcgatta cgactcgcaa tactttgtcc
     2161 gaaatgctaa aatgctgagt tggcttgggc acaatggtgg tatccacaac gtgtttttag
     2221 gcttatggat gtgctttggc cttattggtg taatactttt ttatcaggca ttgctgcgct
     2281 tgttttttcg gttagctaat cgttcgttta ttgccatacc attgttatac gcattgttgt
     2341 tttcaacttc atacgaagcc tggcttatgg gctcgcttaa cccgtttact gtatatttaa
     2401 ttatgtcgtt agtattgctc gactatgacc accaatttag cacctcgaaa aaaagccttg
     2461 ttt
//
LOCUS       DAOMAA010000240         2462 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_38111_length_2462_cov_2.722892, whole genome shotgun sequence.
ACCESSION   DAOMAA010000240 DAOMAA010000000
VERSION     DAOMAA010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38111_length_2462_cov_2.722892"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..1026
                     /locus_tag="PLO59_04090"
     CDS             154..1026
                     /locus_tag="PLO59_04090"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019947257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0044265 - cellular macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HQV00314.1"
                     /translation="MIVPKGKLISIGGAEDKGTEPEPDFLQKNNLNFFELQILQRIVQ
                     EVGGPDKHIEVITSASSIPLEVAHGYLNAFGKLGCSNVQIMDIRKRDEVSHTGYLERI
                     KQCDGVMFTGGNQMRLSTFFGGTPFLEILHQRYRNENFVIAGTSAGAMAMSNTMIYQG
                     SSSDALLKGEVKLSTGLGFMRDVIIDSHFVKRGRFGRLAEAVASNPSCIGIGLGEDTG
                     LLVTEGNYMEAIGSGLVILIDGHNIVHTNIADVQYGSPISIENLIVHVMAKGNGYDLA
                     ARQFYADVKQMQVS"
     gene            1119..1916
                     /locus_tag="PLO59_04095"
     CDS             1119..1916
                     /locus_tag="PLO59_04095"
                     /inference="COORDINATES: protein motif:HMM:NF019106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUMOD4 domain-containing protein"
                     /protein_id="HQV00315.1"
                     /translation="MTNDKTYLGEKWKKVELGVESTNSIVLEISNFGRVRSTTRFFSK
                     RILKGSMINGYKNISLKLFEPRSKAVETRLAYYKKQMALLNKRIAETTKQVKTKGISA
                     KQKRDSQRLLADDTALLNGINAKYKKEFRADELSRTINKAFLIHKLVGQNFCRKPNSN
                     YQFVIHLDYNKLNNKYDNLKWATQAEVTQHSATNPLVLKAIKARRGKRIEGANNYKLT
                     ESKVMIIKKRIAEGKPLRTIAKQFKVSETQILRIKRKINWANVKAAM"
     gene            2064..>2462
                     /locus_tag="PLO59_04100"
     CDS             2064..>2462
                     /locus_tag="PLO59_04100"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559651.1"
                     /note="catalyzes the formation of
                     4-imidazolone-5-propanoate from urocanate during histidine
                     metabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HQV00316.1"
                     /translation="MITTSKLDLTKTKTPTGNKISCKGWVQEAALRMLHNNLDPDVAE
                     RPEDLIVYGGTGKAARNVESFHLIVKALRDLEDDETLLIQSGKPVGILPTHPDAPRVL
                     ISNSQLVPHWANWDNFRKLEAQGLTMYGQMT"
BASE COUNT          860 a          420 c          496 g          686 t
ORIGIN      
        1 aaacaagcca ttaaacattg gcataaaacc aataggcgca ggcattttat attgttaaat
       61 aacttcgtta atcagttaaa gttagatgta cacaattaaa aggttcttat atttttgctg
      121 cctttaattt aaaagtaaac aagtttaaac ggtatgattg tacctaaagg taaattaatt
      181 tcaataggcg gtgccgaaga taaaggcacc gaacccgagc cggatttttt acagaaaaac
      241 aatttgaatt tttttgaatt gcaaattttg caacgcatag tgcaagaagt tggtgggccc
      301 gataaacata tcgaagttat tacctcggca tcgagcatac cgttagaagt tgcacacggt
      361 tacctaaatg cttttggcaa actgggatgc tccaatgtgc agataatgga catacgcaag
      421 cgcgatgagg tttcgcatac aggttatctt gaacgtatta agcaatgtga tggtgtaatg
      481 tttacaggtg gtaatcagat gcggcttagc actttttttg gaggtacgcc ctttttagaa
      541 attttacacc aacgttatcg taacgaaaat tttgtaattg caggtaccag tgcgggggca
      601 atggctatga gtaataccat gatttaccag ggcagcagca gcgatgcatt attaaaaggt
      661 gaagttaaac ttagtaccgg attgggcttt atgcgcgatg taattataga ctcgcatttt
      721 gtaaaacgag gcaggtttgg ccgtttagcc gaagcagttg caagcaaccc atcctgtatc
      781 ggaatcggat tgggtgagga taccggtttg ttggttaccg aaggtaatta tatggaagca
      841 attggaagcg gtttggtaat tttaattgat ggccataaca ttgtgcatac caacattgcc
      901 gatgtacaat atggatcgcc aatcagtatt gaaaatttaa tagtacacgt aatggcaaaa
      961 ggcaatggat atgatttagc agcccggcaa ttttatgccg atgtaaaaca aatgcaagta
     1021 agctaaccct ttaattgatt cgatactttg atgtataaga ccatattaat attttattac
     1081 ttttaatttc tttgtttaca ataaatactt ttcaaaaaat gacaaacgat aaaacttatt
     1141 taggcgaaaa atggaagaaa gttgaacttg gtgttgaaag tactaattcc attgtattag
     1201 aaatatcaaa cttcggcagg gtaagaagca caacacgttt tttttcgaaa cgaattctaa
     1261 aaggttcgat gattaatggt tataaaaata tatcgctgaa actttttgaa ccacgctcga
     1321 aggctgttga aaccagactg gcttattata aaaagcaaat ggcattgtta aacaagcgca
     1381 ttgccgaaac caccaaacaa gttaaaacaa aaggtataag cgctaagcaa aaacgcgata
     1441 gccagcgttt acttgccgat gataccgccc tgttaaatgg cataaacgca aaatataaaa
     1501 aggagttccg ggccgatgaa ttaagccgta ctattaataa agcgtttctg atacataaat
     1561 tagttggtca aaatttttgc cgtaaaccca acagcaatta tcaatttgta attcatttag
     1621 attataataa attaaataac aaatacgata acctaaagtg ggcaacacaa gccgaagtta
     1681 cgcaacactc agcaaccaat ccattggtac taaaagcaat taaagcccgt agaggtaagc
     1741 gtattgaagg tgccaacaat tataaattaa ccgaatcgaa ggtgatgata attaagaagc
     1801 gaattgccga aggtaaaccg cttcgtacta ttgctaaaca atttaaggtt tcagaaacac
     1861 agatattgcg cattaagcgc aaaataaatt gggcaaatgt aaaggcagca atgtaaaaca
     1921 tgctgtgttt ataatttgac aattgcatct ataaattgtt gcatattttt caatgtaagc
     1981 agttgtaatt actgcattta aatgcagaac acataaccca attagaattc gatttacaat
     2041 tatagcattt aatcagcata aaaatgatta ctacatccaa acttgatttg acgaaaacga
     2101 agacaccaac aggcaataaa atatcatgca aaggttgggt gcaagaagcc gcattacgca
     2161 tgttgcataa caacctggat cctgatgttg ccgagcgccc cgaagattta attgtttatg
     2221 gtggcaccgg taaagcggct cgcaacgtag agtcgttcca tttaatagta aaagcattac
     2281 gcgatttaga agatgacgaa acgctattaa tacaaagtgg caaaccggtg gggatattac
     2341 caacacaccc cgatgcaccc agggttctga taagcaattc gcaattggta ccgcattggg
     2401 caaattggga taactttaga aaattagaag cacaaggact taccatgtat ggccaaatga
     2461 ct
//
LOCUS       DAOMAA010000241         2457 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_38255_length_2457_cov_2.758118, whole genome shotgun sequence.
ACCESSION   DAOMAA010000241 DAOMAA010000000
VERSION     DAOMAA010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38255_length_2457_cov_2.758118"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /gene="rplB"
                     /locus_tag="PLO59_04105"
     CDS             complement(<1..378)
                     /gene="rplB"
                     /locus_tag="PLO59_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448275.1"
                     /note="one of the primary rRNA-binding proteins; required
                     for association of the 30S and 50S subunits to form the
                     70S ribosome, for tRNA binding and peptide bond formation;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HQV00317.1"
                     /translation="MAIKKLRPITPGQRFMARSTFDEVTTNRPEKSLTQSMNRTGGRN
                     NTGKMTMRYIGGGHRKIYRVVDFKRDNTGVPGKVDSIQYDPNRSAYIALIHYVDGDKR
                     YIIAANGLKVGQSVVSGAGAAPEV"
     gene            complement(404..694)
                     /gene="rplW"
                     /locus_tag="PLO59_04110"
     CDS             complement(404..694)
                     /gene="rplW"
                     /locus_tag="PLO59_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HQV00318.1"
                     /translation="MNIIIKPIITEKVTKLGEKAPRYGFFVNPDANKIQIKNAVAQMY
                     NVSVASVNTIIQRGKSKSRYTKAGFVSGKRSNVKKAIVTLKAGDAIDFYANI"
     gene            complement(739..1365)
                     /gene="rplD"
                     /locus_tag="PLO59_04115"
     CDS             complement(739..1365)
                     /gene="rplD"
                     /locus_tag="PLO59_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HQV00319.1"
                     /translation="MELSVYNIEGKDTGRKVNLSSNIFITEPNDHAIYLDVKQYLANQ
                     RQGTHKSKERAEIARTTKKLKRQKGTGGARAGSMKSPLFKGGGRAFGPQPRDYSFKLN
                     KKLKQLARKSALGYKLQANAITIIEDFTLDAPKTKTYVGMIGNLQLAGKKTLMVVGDS
                     NKNIYLSSRNLERNKVVSASSLNTYDIMNAQQIVVSEKSLTLIESTLA"
     gene            complement(1411..2028)
                     /gene="rplC"
                     /locus_tag="PLO59_04120"
     CDS             complement(1411..2028)
                     /gene="rplC"
                     /locus_tag="PLO59_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HQV00320.1"
                     /translation="MSGIIGKKVGMTSIFDAEGRNVPCTVIQAGPCVVTHVKTVEKDG
                     YQALQLAFDEKTEKHTPKAMQGHFAKASTTPKRKLAEFKGFEAEYNLGDVIKADLFAE
                     GEFCDAIGVSKGKGFQGVIKRHGFGGVGGTTHGQHNRLRAPGSLGASSWPSRVFKGMR
                     MAGRTGFDNVKIQNLEVIKVIAEHNLILIKGAVPGHKGSYVLLEK"
     gene            complement(2116..2421)
                     /gene="rpsJ"
                     /locus_tag="PLO59_04125"
     CDS             complement(2116..2421)
                     /gene="rpsJ"
                     /locus_tag="PLO59_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HQV00321.1"
                     /translation="MSQRIRIKLKSYDFNLVDKSAEKIVKTVKSTGAVVSGPIPLPTE
                     KQIYTVLRSPHVNKKAREQFQLCAYKRLLDIHSSTAKTVDALMKLELPSGVEVEIKV"
BASE COUNT          658 a          483 c          425 g          891 t
ORIGIN      
        1 tacttcaggt gctgcaccag ctcctgatac aacactttga ccaactttaa gtccatttgc
       61 agctataata tagcgcttat caccgtcaac ataatgtatt aatgcaatat aagctgaacg
      121 atttggatcg tattgaatcg aatctacttt tccgggtacg cctgtattat cacgtttaaa
      181 atctacaaca cgataaattt tacgatgacc accaccaata tagcgcatag tcattttacc
      241 ggtattattt cttcctccgg tacgattcat actttgggta agactttttt caggtcggtt
      301 agtcgtaact tcgtcaaatg tagaacgtgc cataaacctc tgaccaggag ttatcggacg
      361 tagtttttta attgccattg cttactttat cgtttctctt gttttatatg tttgcgtaaa
      421 agtcaattgc atcgcccgct ttaagggtaa cgattgcttt tttcacatta cttcttttgc
      481 cgcttacaaa accagctttt gtataacgtg attttgattt accccgctgg attattgtat
      541 taactgatgc aaccgataca ttatacattt gtgcaactgc attcttgatt tgaattttgt
      601 tcgcatccgg atttacgaaa aaaccgtatc gcggtgcttt ttcaccaagt ttggttactt
      661 tttcggtaat gatgggtttt ataataatgt tcatttcgcg ctcttttttc ttttgatgat
      721 aatgattttt gaaaaatttt aggctaatgt gctttcaatt aacgtcaaac tcttctcaga
      781 aaccacaatt tgttgcgcat tcataatatc gtaagtgttt aaagatgagg cacttacaac
      841 cttattgcgc tccaaatttc gggacgacaa atatatattt ttatttgaat cgcctactac
      901 cattaaggtt ttttttccgg ctaattgcaa attacctatc atacctacgt atgttttggt
      961 ttttggtgcg tccaaagtaa aatcctcgat aatggtaatt gcattagctt ggagtttata
     1021 gcccaaagct gatttacgag ctaattgctt taatttttta tttaacttaa atgaatagtc
     1081 gcgtggctgc ggtccaaaag cacgacctcc acctttaaat aaaggcgact tcatactacc
     1141 agccctggca ccacctgtac ccttttggcg tttaagtttt ttggttgttc gtgcaatttc
     1201 agcacgttct tttgacttat gtgttccttg gcgttgattg gccaaatatt gttttacatc
     1261 taagtagatg gcatggtcat taggttcagt aataaatata ttactgctta agttaacttt
     1321 gcgacctgtg tcttttcctt cgatattata aactgataat tccattgtta agaaattgaa
     1381 tggtatttga ttgattgatg ataggggcta ttatttttct aataaaacat agctgccctt
     1441 atgccctggc acagcacctt taataagtat taagttgtgc tcggcaataa cttttattac
     1501 ctccagattt tgaattttta cattatcaaa accggtacga cccgccatac gcattccttt
     1561 aaatacgcgt gaaggccaag acgatgcgcc taatgaacca ggtgctctta ggcggttgtg
     1621 ttgaccatga gttgtaccac caacaccgcc aaagccatga cgtttgataa caccttgaaa
     1681 acctttaccc ttacttacgc caatagcatc acaaaattca ccttcagcga aaagatctgc
     1741 ttttattaca tcacctaagt tgtactccgc ttcgaaacct ttaaattcgg ctaacttgcg
     1801 ctttggtgtt gtactggctt ttgcaaaatg accttgcatt gcctttggag tatgtttttc
     1861 agttttttca tcaaaagcta actgaagtgc ttgataaccg tctttttcta cggtttttac
     1921 atgagttact acgcatgggc ctgcttgaat aactgtgcaa ggtacattgc gtccttcagc
     1981 atcaaagatg ctggtcatgc ctactttttt tcctataatt cctgacattt tataaatagg
     2041 ttgtaattag tggtctctgc ttcaaaagtc ggagacataa ttgtttttcg attttaatga
     2101 acgtatttaa attaattaga ctttaatttc aacttcaaca ccacttggca attcaagttt
     2161 cattaaggca tcaactgttt ttgcagttga gctatgtata tctaacaaac gcttatatgc
     2221 acataactga aactgttcgc gcgccttttt gtttacgtgt ggtgagcgaa gcacggtgta
     2281 aatttgtttt tctgttggca atggaattgg cccactaaca acagcaccgg ttgactttac
     2341 tgtttttaca attttttcgg ctgatttatc aaccaaatta aaatcatatg atttcagttt
     2401 gatacgaatt cgttgactca ttgcttgttt atttaaagaa cgttattgta taagatt
//
LOCUS       DAOMAA010000242         2448 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_38539_length_2448_cov_2.626410, whole genome shotgun sequence.
ACCESSION   DAOMAA010000242 DAOMAA010000000
VERSION     DAOMAA010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38539_length_2448_cov_2.626410"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..811
                     /locus_tag="PLO59_04130"
     CDS             140..811
                     /locus_tag="PLO59_04130"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HQV00322.1"
                     /translation="MIFGFLSDAHSHTESFFKGYRYLQTLCDKIFYLGDFVGYFPVSN
                     QVIDAIRTDGIVSVIGNHDAMLLGYLPINPERDKIYQLAKTRNAISKINYDYLNSLPT
                     SIEINSGNEKLLLMHGSPFDHLNGYIYPNSDLNPFTALAPDVFFMGHTHRPFYSKEQN
                     KLIVNVGSVGFSRIAGNIINVVLYDTVSHAVTIKFIEVNVYQILTRYKNHLHPTLQNI
                     LRRDL"
     gene            1014..>2448
                     /gene="asnB"
                     /locus_tag="PLO59_04135"
     CDS             1014..>2448
                     /gene="asnB"
                     /locus_tag="PLO59_04135"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HQV00323.1"
                     /translation="MCGICGIISFDNASVKKQQLMQMTTALAHRGPDGEGFWISDNAR
                     VGFGHRRLSIIDLTQSASQPMSFANGRLMITFNGEIYNYIELRNQLLKLGYQFKSDSD
                     TEVLLALYHLKGEKCLQDLEGMFAFAIWDEEQQKLFCARDRFGEKPFFIYKDGAQFIF
                     ASEIKALFAFGIKRDVNQNNLFNFISLKHTYEPYNRSATFYNQIEKLEAAHYLIVDSN
                     GKISKTKYWDIDVDKQQNISFDDACKTFTELFTSSLLKRLRSDVPIGSSLSGGLDSSV
                     IVCLIDALNKGHLQQSTFSARFKNFEKDEGYFMQQVIDKTNVDPHFIFPDEDTFLDSF
                     ERMTYHMEEPFGSASVMAQYAVMELAKKNMITVLLDGQGADESLAGYNYMYYYFFLDQ
                     ARYNRSNLKSELAAYRALFNTDFVRGKLFNLYAMIPSFYNMAIRYKQKQEALSNDFVN
                     KDFAAYCGKNHFEYNQGIANKFNHNLSG"
BASE COUNT          816 a          375 c          452 g          805 t
ORIGIN      
        1 atgcgcataa atggatgcat tacataaata tttatatgca tattttcaat ataaatgcaa
       61 cccggaaagc agttttaatt tgaagtgata aattaataaa tagcttagct acttacaata
      121 tttcgaagaa actgcaaata tgatatttgg atttttatca gatgcccata gtcataccga
      181 atcttttttt aaaggctatc gttatttgca aacattatgt gataaaatat tttatttagg
      241 cgattttgta ggatattttc cggtaagtaa tcaggtaatt gacgctatca gaacggatgg
      301 tattgtaagc gtaataggca atcatgacgc tatgcttttg ggttacttgc ccattaaccc
      361 cgaacgcgat aaaatatatc aacttgccaa aacaagaaat gcaatttcaa aaataaatta
      421 tgactattta aattctttac caacaagtat tgaaataaat tcaggaaatg aaaagttgtt
      481 acttatgcat ggcagtccgt tcgatcattt aaacggttac atatatccta acagtgattt
      541 aaacccattt acagcattag caccggatgt tttttttatg ggacatacgc atcgcccatt
      601 ttatagcaag gaacaaaaca aactgattgt aaatgtaggc tcagttgggt tttcgcgtat
      661 tgcaggtaat attattaatg tggttttata tgataccgtt tcgcatgctg taaccattaa
      721 gtttatcgaa gtaaatgttt atcagattct gacacgttat aaaaaccatt tgcatccaac
      781 attgcaaaat atattaaggc gcgatttata atggcaaata atttaaaacg atagtgcgtt
      841 aaaaattggc acctcgtggt gcagttattc tatcgtttat aaacaaaagc aaatattaaa
      901 tgatgttata caatagcttt gaggtttgtt gaaataactt actttgcgca acttattgtt
      961 actgctttta ttttaaaaac aacttacttt ttagcagcaa ttcattacaa cccatgtgtg
     1021 gtatttgtgg tataatttca tttgataatg catcagtaaa aaagcagcag ctcatgcaaa
     1081 tgacaactgc tttggcacac agaggccctg atggcgaagg cttttggata agcgataatg
     1141 cacgcgtggg gtttggccac cgcagattaa gcattattga tttaacgcaa agtgccagcc
     1201 agcctatgtc atttgcaaat ggacggttaa tgataacttt taatggcgaa atttataact
     1261 acattgagtt aagaaatcaa ttattaaaac tcggctatca atttaaatcg gatagcgata
     1321 cagaagtgct gttagcttta taccatctta aaggcgaaaa atgcctgcag gatttagagg
     1381 gtatgtttgc ttttgctatt tgggatgaag aacaacaaaa acttttttgt gctcgcgaca
     1441 ggtttggcga aaaaccattt tttatataca aggatggcgc tcagtttatt tttgcatccg
     1501 aaattaaagc gctttttgct tttggtataa agcgcgatgt aaatcaaaac aaccttttta
     1561 attttatttc attaaagcat acgtatgaac cttacaaccg ttcagctacg ttttataatc
     1621 aaatcgaaaa attagaagct gcacattatc tgatagtaga tagtaatggt aaaatttcga
     1681 aaacaaaata ttgggatatt gatgttgata agcaacagaa tatttcattt gatgatgctt
     1741 gtaaaacatt tacagaatta tttaccagct cattattaaa aaggttacgt tctgatgtgc
     1801 caataggttc aagtttatcg ggtgggttag atagctcggt aatagtatgt ttaatagatg
     1861 cactaaacaa agggcatttg cagcaaagta ctttttcggc aaggtttaaa aatttcgaaa
     1921 aggatgaagg gtattttatg caacaagtaa ttgataaaac caatgttgac ccgcatttta
     1981 tttttccgga cgaggatacg tttttagatt cattcgaacg tatgacatac cacatggaag
     2041 aaccgtttgg gtcggccagt gttatggccc aatatgctgt aatggagtta gcaaaaaaaa
     2101 atatgattac tgttttattg gatgggcaag gtgctgatga aagtttggcc ggatataact
     2161 acatgtatta ttactttttt ttagaccaag cccgttataa ccgtagcaat ttaaaatcgg
     2221 agttggcggc ttatcgagca ttgtttaata ctgattttgt tagaggaaag ttgtttaatt
     2281 tatatgccat gattccatct ttttacaaca tggccatacg ctataaacaa aagcaggaag
     2341 cattatcaaa cgatttcgtt aataaggact ttgccgctta ttgtggaaaa aatcattttg
     2401 aatacaatca gggaattgcc aataaattta accacaacct ttcaggct
//
LOCUS       DAOMAA010000243         2442 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_38703_length_2442_cov_2.736489, whole genome shotgun sequence.
ACCESSION   DAOMAA010000243 DAOMAA010000000
VERSION     DAOMAA010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38703_length_2442_cov_2.736489"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="PLO59_04140"
     CDS             complement(<1..489)
                     /locus_tag="PLO59_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HQV00324.1"
                     /translation="MKQIEIVLLPQQAATEDSIEQNLRQYLGIRPDDVLFYQIQKKSI
                     DARNRQVKIRLQLVYSLKLPLPAMAIANRVLQHVANAMPVAVIGCGPAGLFAALRLIE
                     LGYKPVIIERGKDVKSRRRDLALLNKQGLVNPESNYCFGEGGAGTYSDGKLYTRSNKR
                     GNV"
     gene            complement(632..1111)
                     /locus_tag="PLO59_04145"
     CDS             complement(632..1111)
                     /locus_tag="PLO59_04145"
                     /inference="COORDINATES: protein motif:HMM:NF025491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 domain-containing protein"
                     /protein_id="HQV00325.1"
                     /translation="MIQRKQSIFLLLVILLSVALFIPAIAPFAYVFSGSSKEFDINLN
                     ITGVYTKCVSINEPASYTALMAINSLIILLTLYCIFRYNKRMIQLKLCYVIAVFVILL
                     MAGMYMFTNLMMRKTADAHSVYLIGYYLPLIQLVLLFMAGKYIKADEDLVRSSDRLR"
     gene            1260..2054
                     /locus_tag="PLO59_04150"
     CDS             1260..2054
                     /locus_tag="PLO59_04150"
                     /inference="COORDINATES: protein motif:HMM:NF025221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HQV00326.1"
                     /translation="MKKLIIAILFCYTQTYSQIKLEVIDPPKTSGSNSSTQTTTTNHT
                     SNTGSITNNASANKNLGRGLGNDVVVDGLREALTLASQNAGNKAGLKDGFFKNNLIKI
                     PFPPEIKDIEKTVRTLGMSKQADKFIESLNRAAEDAAKKAAPIFINAVKGMSIQDGLS
                     ILQGGNDGATQFLKTNTQTELSTAFAPVVKNSLSKTQVTKYWKDVAKTYNKVPFAKPI
                     NPDLEKYVTERAISGLFTLVAQEELKIRQDPMGQASNLLQTLFGGK"
     gene            2222..>2442
                     /locus_tag="PLO59_04155"
     CDS             2222..>2442
                     /locus_tag="PLO59_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HQV00327.1"
                     /translation="MSTDTANNATEINNTPADANQNQTETATNNQPETATENNAELTL
                     AQAEAQTYKDKFVRLTAEFDNYKRRTLRER"
BASE COUNT          862 a          495 c          425 g          660 t
ORIGIN      
        1 cacgttgcca cgcttgttgc tgcgtgtata aagcttacca tccgagtaag tacctgcacc
       61 accttcgcca aaacaataat tcgactcggg gttaaccaag ccttgcttgt taagtagcgc
      121 caaatcgcgt ctgcggcttt ttacatcctt acctcgttcg ataataacag gtttataacc
      181 cagttcgata aggcgcaatg cggcaaacaa gcctgcagga ccacaaccaa ttacagcaac
      241 aggcatggca ttggctacgt gttgcaaaac cctgtttgct atagccattg ccggcaaagg
      301 caattttaaa ctatacacaa gttgcaatct tatttttact tgcctgtttc gggcatcaat
      361 tgattttttt tgtatctgat aaaacaaaac atcatcgggc cttatgccta aatattgtct
      421 taggttttgc tcaatgctat cttctgttgc cgcttgttgc ggaagcaaaa ctatttctat
      481 ttgcttcatg tttattggct ttaaatatgc atttcagata acatgataca agtgataaaa
      541 ttcatcagat aaaataaaac aagcactaat taattgcgca aatcttaggc aggcaatcat
      601 aaaatttaaa ctgctactta cttgcaacag gttacctcaa acggtcggaa ctacgcacca
      661 aatcttcatc cgctttaatg tatttaccgg ccataaataa aagtaccaat tgaattagcg
      721 gcaaatagta tccaattaaa taaactgaat gcgcatcggc tgttttacgc atcattaaat
      781 tagtaaacat atacatgccg gccattaaca aaataacaaa cactgcaatt acataacaca
      841 gttttaattg tatcatacgt ttgttgtatc tgaaaatgca ataaagagtc agtaaaataa
      901 tcaaactgtt aatggccatt aatgctgtat aacttgctgg ttcgttaatg cttacacact
      961 ttgtataaac accggtaata ttcaggttaa tatcaaactc cttgctactg cccgaaaaca
     1021 cataagcaaa tggtgcaatt gccggaataa ataaagctac actaagtaaa attacaagca
     1081 gcagaaatat agattgttta cgttgtatca tgcagcaaat taaagcaatg tgttttaaag
     1141 tcaaattaaa aatattgccg ctatttttta ataaggcaca ttcactatgt attcattaat
     1201 agttttgcag gtggctacat ttttgcaaac aggccttcaa tcattttaaa aaacaaacca
     1261 tgaaaaaatt aattattgcc atactgtttt gctacacgca aacgtacagc caaattaaac
     1321 tcgaagttat tgacccaccc aaaacatcgg gcagcaatag tagcactcaa acaacaacta
     1381 ctaaccacac ttcaaataca gggtcaataa ccaataacgc aagtgccaat aaaaacctgg
     1441 gacgtggttt aggtaatgat gtagtggttg atggcctgcg cgaagcttta acattggcaa
     1501 gccaaaatgc aggtaataaa gccggcttaa aagatggatt ttttaagaac aacctgataa
     1561 aaattccgtt tccacccgag attaaagaca ttgaaaaaac ggtacgaact ttaggtatga
     1621 gcaaacaagc cgataagttt attgaaagtc tgaaccgtgc tgccgaagat gccgccaaaa
     1681 aagcagcacc catttttata aatgccgtaa aaggtatgtc aattcaggat gggctctcaa
     1741 tactacaagg cggtaatgat ggtgctacgc aatttttaaa aacaaataca caaaccgagt
     1801 taagtacagc ctttgcaccg gtggttaaaa actcattatc aaaaacacag gtaacaaaat
     1861 attggaaaga tgtagccaag acctataaca aagttccgtt tgcaaaacca ataaaccccg
     1921 atttggaaaa atatgtaacg gagcgtgcta taagcggctt gtttactttg gttgcgcaag
     1981 aagaattaaa aatacgacaa gaccctatgg ggcaggcaag taatttgctt caaaccttat
     2041 ttggcggcaa gtaaaagttt aaaaacttct taaaaagccc atgaccggta gtttgcctca
     2101 tgggcttttt tacgttaggt ggttgtaaat attacattcg cggcctgtca atttgacccc
     2161 ataaatagcg tggcaatata tttgaaaacc tatgttcaaa ttcaaaaaaa acaagacgaa
     2221 tatgagtacc gatacagcta ataatgctac tgaaattaat aatacacctg ctgacgcaaa
     2281 tcaaaatcaa accgaaaccg ctaccaacaa tcaacctgaa actgcaacag aaaataatgc
     2341 agagcttacc ttagcccaag cagaagcaca aacctataaa gataaatttg taagacttac
     2401 ggcagagttt gacaactaca aaaggcgcac cttgcgcgag cg
//
LOCUS       DAOMAA010000244         2437 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_38849_length_2437_cov_2.717884, whole genome shotgun sequence.
ACCESSION   DAOMAA010000244 DAOMAA010000000
VERSION     DAOMAA010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38849_length_2437_cov_2.717884"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..>2437
                     /locus_tag="PLO59_04160"
     CDS             48..>2437
                     /locus_tag="PLO59_04160"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HQV00328.1"
                     /translation="MKTLTIYKSSAGSGKTYTLVFEYLKLVLQNPAAYKHVLAVTFTN
                     KATDEMKQRIVATLIDITNLKQQALVIVLAQQTGIDETQIVTAAQAVLNLILHNYDAF
                     AIQTIDSFFSKTTKALARELNLPLDAETELNQKKIQQQITSSLIDEVGKNELVSKILI
                     DFVKNNIEEDKDWNVSNRISDVSDELFKENYDTKQKLISFEKIDELKAFKKSFEKEMA
                     RLGQLFLTTIKNQNLTISDFKHKGNGVAGYFVKISKRCEPDDYTPGVRFLAALNDDVW
                     FANSAGDKSEIDTKPFNQIAKQVSDYYDAHATNYFTTVCILKLALFSLVLDALNTHLK
                     TYRAKNNILLMHDINNTIAHFISASDTPFIYEKTGNRYQHFLIDEFQDTSNIQWNNFL
                     PLIENAMSMGYKSLVVGDVKQSIYRWRGGNMQLLHKKIFSDMQHMSHSIDIKNLNTNY
                     RSKEAIVQFNNSLFAGAPAAFRNFIHPNLSDLLNDTYYKEQVYQQTAEKNIVGGYVNI
                     SFLNLKNNSHNDDVDNEAETTNFVNTALESMLNNIHALQKQNYTLGDIAILVRTNSDG
                     EKVAEFLLSNGIEKVITSDSLSLKGNSKVELLIAALQYAYTDNEVALHTLYIHYNQCR
                     EQFTMPDFSNTSDISHLLNFIETQKQLHLYSFFENVAAYTGLFNTADVYVQRLLDVVL
                     AFCETNLPIPQLFLDYWQKSDAKELNVVLPADANSINIITLHKSKGLQFPVVLMPLIN
                     YSIKPKHKEIIWAPISAAPFNDVDIYPVYAIKLTEQSHFQDKYAQEKSLMLIDNIN"
BASE COUNT          889 a          433 c          413 g          702 t
ORIGIN      
        1 ccgttgtagg caatttaaga cagcatccac ttctaaaata aacaaccatg aaaacactaa
       61 ccatctataa atcatcagca ggttcgggca aaacctatac gctggtgttt gaatatttaa
      121 aattagtgtt acaaaatcca gctgcatata aacatgtttt ggcagttact tttaccaaca
      181 aagcaactga cgaaatgaag caacgcatag ttgcaacgtt aattgatata accaacctga
      241 aacaacaggc actggtaatt gttttagccc aacaaacagg cattgatgaa actcaaattg
      301 taacagcagc acaggcagta ctaaatttaa ttttgcataa ctacgatgca tttgctattc
      361 aaaccattga tagttttttt agtaaaacta ccaaagccct ggcacgcgaa ttaaatttac
      421 ctcttgatgc agaaacagaa ctgaatcaaa aaaaaataca acaacaaata acttcaagct
      481 taattgatga agtaggtaaa aacgaattgg tttcaaaaat tctgattgac ttcgtaaaaa
      541 acaatatcga agaagataaa gactggaacg tatcaaaccg catttcggat gtatctgatg
      601 agttgtttaa ggaaaattac gatacgaaac aaaaactcat cagttttgaa aaaattgatg
      661 agttaaaagc atttaaaaaa tcgtttgaaa aggaaatggc acgcttgggc caactgtttt
      721 taaccactat aaaaaaccaa aaccttacca ttagtgattt taagcataaa gggaatggcg
      781 ttgcaggtta ctttgtaaaa attagtaaac gctgcgagcc tgatgattat acgccaggtg
      841 taaggttttt agctgcttta aatgatgatg tttggtttgc gaattcggct ggtgataaat
      901 cggaaatcga caccaaaccg tttaaccaaa ttgcaaaaca agtatctgat tactatgatg
      961 cacatgcaac caattatttt accaccgttt gtattttaaa actggcactt tttagtttgg
     1021 ttttagatgc attaaataca catcttaaaa catatcgcgc taaaaacaat atattgctta
     1081 tgcacgatat caataacact attgcccatt ttataagtgc aagcgacact ccgtttattt
     1141 atgaaaaaac aggcaaccgg taccaacatt ttcttattga cgagtttcag gataccagta
     1201 atattcaatg gaataacttt ttgccactca tcgaaaatgc aatgagtatg ggctataaat
     1261 cgttggtggt gggcgatgtg aagcaaagta tttatcgttg gcgtggcggc aatatgcagt
     1321 tgctgcataa aaaaatattt agcgatatgc agcacatgtc gcatagtatt gatataaaaa
     1381 atcttaatac caattaccga agtaaagaag ccattgttca atttaataat agcttatttg
     1441 ccggagcgcc tgctgctttt agaaatttta tacacccaaa cttaagcgac ttgttaaacg
     1501 acacctacta taaagaacaa gtttaccagc aaacagcaga aaagaatata gtaggtggat
     1561 atgtgaacat ttcgtttctt aatttgaaaa acaacagcca taacgatgat gtcgataacg
     1621 aagccgaaac aacaaacttt gtaaacacgg ctttagaaag tatgttaaat aatatacatg
     1681 ctttacaaaa acaaaattac acattgggcg atatagctat attggttaga accaattccg
     1741 atggcgaaaa agttgcagaa tttttactaa gtaacggtat tgaaaaggta atcacatccg
     1801 attcattatc acttaaaggc aatagtaaag ttgaattgct aattgctgct ttacaatacg
     1861 catacaccga taacgaagtg gctttacaca cactatacat acattacaat caatgccgcg
     1921 agcaatttac aatgcctgat ttttcaaaca catcagacat cagtcatttg ctaaacttta
     1981 tagaaaccca aaaacaactg cacctgtata gttttttcga aaacgttgct gcctatacag
     2041 gcttgtttaa tacggccgat gtatatgtac aaaggttgtt agatgttgta ctagcttttt
     2101 gtgaaaccaa tctgcccata ccacaattat ttttagatta ttggcaaaag agcgatgcaa
     2161 aagaattaaa tgtagtattg cctgccgatg caaatagtat aaacatcatt acattacata
     2221 aatcaaaagg gcttcagttt ccggtagttt taatgccctt aatcaattac agtataaaac
     2281 ccaaacacaa ggaaattatt tgggcgccca tatctgctgc accctttaat gatgtggata
     2341 tatatccggt ttatgcaatc aagcttactg agcaatcgca ctttcaagat aaatatgcgc
     2401 aagaaaaatc gctgatgctg attgataata taaacat
//
LOCUS       DAOMAA010000245         2436 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_38878_length_2436_cov_3.249475, whole genome shotgun sequence.
ACCESSION   DAOMAA010000245 DAOMAA010000000
VERSION     DAOMAA010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38878_length_2436_cov_3.249475"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1162)
                     /locus_tag="PLO59_04165"
     CDS             complement(<1..1162)
                     /locus_tag="PLO59_04165"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HQV00329.1"
                     /translation="MIKQSELLKKWFGLLCLKLLFACTLGYGQGGSTSNPHVLPGFTD
                     DKVSDGWDDLRQLVFDSLGRMFVVEGAGEIWIVDTNGVKINAPLLNIKEEVGQWSSHG
                     LMGLALDKNFLTNGYFYVMYTVDRHHLLEFGTPNYNANVNNFFSATINRVTRFQADPT
                     TNFTTLIPNSRFVLIGQTKQTGIPVTYTFHDGGHIEMMLDGSLVISTGDNAHGGSVDT
                     GSNAQTYYAQCLLDSILILQTNIGGFRSQLIQSLNGKLLRIDPMTGLGISSNPYYDAA
                     QPNAPISKVWCLGLRQPYQFCIKPGTSSTDITAGLPGTFYIGDVGFYKWESLFICDSA
                     AQNFGWPSYEGMAPHNGFINKWVPNYYAPNPLFGTGGCTQPYFYFKDLLLEPT"
     gene            1537..1995
                     /locus_tag="PLO59_04170"
     CDS             1537..1995
                     /locus_tag="PLO59_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00330.1"
                     /translation="MNLTQGLQYGFITQELEMVLPQLMRQFVQPPTQDSSGNIITPQV
                     NFTAIAYQPLIALLIAGYKEHQQQIDSLADLINNNAYPLKQPLQSMTVTLSNQASIIL
                     NQNDPNPFAQNTTITYSIPDEVKNAQLLIYDNNGTILKTITINEKGQAVF"
     gene            complement(2146..>2436)
                     /locus_tag="PLO59_04175"
     CDS             complement(2146..>2436)
                     /locus_tag="PLO59_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HQV00331.1"
                     /translation="HRDHAYEFGKKICEKLKELMTRQQFEIIIQASIGAKIIARETVK
                     ALRKDVTAKCYGGDISRKRKLLEKQKEGKKRMRQVGNVEIPQAAFMAVLKLD"
BASE COUNT          777 a          494 c          434 g          731 t
ORIGIN      
        1 gtgttggttc taacaatagg tctttgaaat agaaataagg ttgggtgcaa cctccggtgc
       61 caaataatgg attgggtgca taatagttgg gtacccattt attaatgaaa ccattatgtg
      121 gcgccattcc ttcataactg ggccaaccaa agttttgtgc tgcgctatcg caaataaata
      181 aactctccca tttataaaaa cctacatcgc caatgtaaaa tgtacctgga aggccggctg
      241 taatatcagt tgatgatgtg ccgggtttaa tacaaaattg ataaggttgt cgaagcccca
      301 aacaccaaac tttgctgatg ggtgcattgg gttgtgctgc atcgtaataa gggtttgaac
      361 ttatgcccaa tccggtcatt gggtcaatac gtaacaactt gccgtttaaa gattgaatta
      421 actgtgacct gaagccacca atattggttt gcagtatcaa aatgctatct aacaaacact
      481 gggcatagta ggtttgtgca ttgctgcctg tatctaccga accaccatgt gcattatcgc
      541 ctgtagaaat tactaacgac ccgtctaaca tcatttctat atggccacca tcatgaaagg
      601 tataggttac aggtatgcct gtttgtttag tttggccaat taaaacgaag cggctgttag
      661 gtattagcgt agtaaaattg gtagttgggt cggcctgaaa acgggtaacc cggttaatgg
      721 tagcgctaaa aaagttattt acattggcat tataatttgg tgtaccaaac tctaaaaggt
      781 gatgcctgtc aacagtgtac attacataaa aatagccgtt ggttaaaaaa tttttatcca
      841 aggctaagcc cattaaacca tgcgagctcc attgacctac ttcttcttta atatttaaaa
      901 gtggggcatt tatttttacg ccattggtat caacaatcca aatttcgcct gcaccctcaa
      961 ctacaaacat gcgacctaat gagtcgaata ccaattggcg caaatcgtcc catccatcag
     1021 atactttatc atctgtaaag cctggtaaaa catgcggatt acttgttgag cctccttgtc
     1081 catatcccaa tgtgcaagca aataacaact tgagacacaa aaggccaaac cattttttta
     1141 gtaattcact ttgcttaatc atgacaataa tttaaaatca gataacacta aatgctgatg
     1201 tttagaaatt aattttctgt ataaaaatta aaagataaac atcatgcgaa agtaaacaat
     1261 tgtgtggcaa tctgtttagt tttttagtca gttttttgta aggcatgctt agcgttttcg
     1321 tatccataat cagcccaaat ttggtaagcc aatgaagctt gctaataaat catcagaaaa
     1381 tcaaatgcaa tcagttaata acagcattgc agacattttt ttactgacaa ttgtaagtca
     1441 taatgcttac agccaggttg atgttttaaa tcacgttgga aacaatgctt cttactaagt
     1501 gggttgggat aatgctgcta tgtcaaacac cttaaagtga acttaacaca gggcttacaa
     1561 tatggcttta ttactcagga attggaaatg gtactgccgc aacttatgcg tcagtttgtg
     1621 caaccgccaa cgcaagattc ctcaggaaat attataacac cacaggtaaa ttttacggct
     1681 attgcttacc aaccactaat tgcactttta atagccggat ataaagagca ccaacaacaa
     1741 attgactcct tagctgattt aataaacaac aacgcctatc cactaaaaca gccattgcaa
     1801 tccatgactg ttacgttaag caatcaggca tccatcatac tcaaccaaaa cgaccccaat
     1861 ccctttgccc aaaacactac catcacatat tcaattcctg atgaagtaaa aaacgcacaa
     1921 cttttaattt acgataacaa cggtactata cttaaaacca ttacgattaa cgaaaaaggc
     1981 caagcagtat tttaatttat ggcagccaat tatcaactgg catttacacc tacactttaa
     2041 tagccgatgg caaacccgtt gaaaccaaac gtatggtaaa agtgaagtga ggaaagctcc
     2101 gggtataaaa aattaatttt gtaacgattt tcgtaaatca gttgattaat cgagtttaag
     2161 cacagccata aatgcggctt gcggaatttc gacattccct acctgacgca tacgtttttt
     2221 gccttctttt tgtttttcaa gcaattttcg tttccgcgaa atatcgccac cataacattt
     2281 agcagtaaca tctttacgca aggctttaac ggtttcgcgt gctataattt tggctccaat
     2341 agaagcttgt attataattt cgaattgctg gcgcgtcatc aactctttaa gtttttcgca
     2401 tatttttttg ccaaactcat aagcatggtc gcggtg
//
LOCUS       DAOMAA010000246         2433 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_38953_length_2433_cov_3.888982, whole genome shotgun sequence.
ACCESSION   DAOMAA010000246 DAOMAA010000000
VERSION     DAOMAA010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38953_length_2433_cov_3.888982"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1701)
                     /locus_tag="PLO59_04180"
     CDS             complement(<1..1701)
                     /locus_tag="PLO59_04180"
                     /inference="COORDINATES: protein motif:HMM:NF012975.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HQV00332.1"
                     /translation="MKTSTKNMTPALRTLLKECVATIFKQHGNQPLNSKQLTKIVKHN
                     ESFAPALWQNDIDDIELRDEIMKACYTLVADEIITENQPGRFKLIPETRIVEGVIEIT
                     STGAAYVVNQLHEKDIYIAPNNTGTALNRDTVRVSLYAHRAGRRAEGEVIEIIKRFKT
                     EFAGTLQVSSRHAFFIADGNRMNVDIFIPLQALQGAGNGDKVVVRLTHWPEDSKNPEG
                     EVINILGKPGENNAEMDAILIEYGFPLPFPDVVEKEAAKIPFEIPAAVTKARKDFRKT
                     TTFTIDPADAKDFDDALSFKKLKNGHYEIGIHIADVSHYLTEKMAMEKEAYERATSVY
                     LVDRVIPMLPEKLSNHVCSLRPYEDKLCFSAVFEIDAKANVITEWYGRTVIHSIKRFT
                     YEEAQTVIETGVGDLVDEVHTLNKLAQMMRANRFKNGAINFDRLEVKFNLDEAGNPTG
                     VYTKQMKESNQLIEEFMLLANRSVATFIGKQHATYNTKVSVTQKQLPFVYRVHDLPDP
                     EKVKQLLQFAGKFGYRMKANTETELAHSINKLVKEIKGKGEQNLLEVLAIRTMSKAKY
                     S"
     gene            1842..>2433
                     /locus_tag="PLO59_04185"
     CDS             1842..>2433
                     /locus_tag="PLO59_04185"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HQV00333.1"
                     /translation="MYKFITTAYMFLYCCINVAHAQLTLIIDSVPRYFTPINDTLYVA
                     GTFNNWSPADANFRMQKQSNGTYTVNVTTTGALQFKFTRGAWANNETQLNGNQLANRN
                     YTYNGGIDTLHCNVANWSDYAPGTHTATPNVYVVASNMFMPQLNRWRRVWTYLPNDYY
                     TSNRYYPVVYMHDGQNVFDAFTSFAGEWSVYDTLRNM"
BASE COUNT          672 a          493 c          462 g          806 t
ORIGIN      
        1 gctgtacttt gctttcgaca tggttctgat agcaagtacc tctaataaat tttgttcgcc
       61 tttgccttta atttctttta ctaatttatt gatcgaatgt gccagttctg tttctgtatt
      121 tgctttcatg cgataaccaa actttccggc aaactgtaac agctgtttta ctttttcggg
      181 gtcgggcaag tcatgaactc tgtaaacaaa tggcaactgt ttttgcgtaa cgctaacttt
      241 ggtattatac gtagcatgtt gtttgcctat aaaggtagca acacttctgt tggcaagcag
      301 cataaattcc tctattaact gattgctttc tttcatttgt ttggtatata caccggttgg
      361 gttaccagcc tcgtctaaat taaattttac ctccagcctg tcgaaattaa tggcgccatt
      421 tttaaaacga ttggcacgca tcatttgtgc caacttgttt agtgtatgga cctcatccac
      481 taaatcaccc acaccggttt cgataacggt ttgcgcctct tcgtaggtaa aacgtttaat
      541 cgaatgtatt acagttcggc cataccattc tgtaattacg ttggctttgg catctatttc
      601 aaacacagcg ctaaagcaaa gtttatcttc gtagggcctt aatgagcaaa catgatttga
      661 taatttttcg ggtaacatgg gtataaccct gtcaactaaa taaacggagg tagcacgttc
      721 ataagcttct ttctccatag ccattttttc tgtcagataa tgcgatacgt cagcaatatg
      781 aataccgatt tcatagtggc cgttctttag ttttttgaaa cttaaagcat catcaaaatc
      841 cttggcatcg gctgggtcaa tagtaaacgt ggtggttttt ctaaagtctt tgcgggcttt
      901 agttacggca gcaggtattt cgaatgggat ttttgcggct tctttttcaa ccacatccgg
      961 aaacggaagc ggaaagccat actcaattaa tatggcatcc atctcagcat tattctcacc
     1021 gggtttgcct aaaatattta taacctcacc ttctgggttt ttcgagtctt cgggccaatg
     1081 tgttaatcgt accaccactt tatctccatt gccagcacct tgcaatgcct gtaacggaat
     1141 aaaaatatca acgttcattc tgttgccatc ggcaataaaa aatgcatgcc ggctgctaac
     1201 ttgtagcgta ccggcaaact cggttttaaa gcgttttata atttctatca cttcgccttc
     1261 ggcacgcctg ccggcacgat gtgcatacaa tgaaacgcgc actgtatcgc ggtttagtgc
     1321 agtgcctgta ttattgggtg ctatataaat atctttttca tgcaattgat ttaccacata
     1381 agctgcaccg gttgatgtaa tttcaattac accttcaaca atgcgtgttt cgggtataag
     1441 tttaaaacgc cccggttgat tttctgtaat aatttcatca gctaccaaag tataacaagc
     1501 tttcattatc tcatcgcgta attcaatatc atcaatatca ttttgccata atgcaggtgc
     1561 aaagctttca ttgtgtttta caattttggt tagttgcttg ctgtttaatg gttgattgcc
     1621 atgttgttta aaaatagtgg ctacacattc cttaagcaaa gttcgtaaag ccggggtcat
     1681 attttttgtg cttgttttca tgcctttttt aaatcaggta tagcccgcaa aataataaag
     1741 gcatgttaag ttaatgtgtt taaataaaag tgggttcgta ttattataaa catattttaa
     1801 gcagaagacg cttacattgc aggcgctaaa tattttccaa aatgtataag tttattacca
     1861 ctgcatatat gtttttgtat tgctgcataa acgtagcaca tgcccaacta acattaatta
     1921 ttgatagtgt accacgctat tttacaccca ttaatgacac gctttatgtt gccggaactt
     1981 ttaataactg gagcccggca gatgcaaact ttcgtatgca aaaacaaagt aatggtacct
     2041 atacagtaaa tgttacaaca acaggtgcat tgcagtttaa atttacacgt ggagcctggg
     2101 ctaataacga aacacagcta aatggcaacc aattagccaa ccggaactat acctacaatg
     2161 gtggtattga tacattgcac tgtaacgttg caaactggag cgattatgca cccggtacgc
     2221 acactgcaac acctaatgta tatgttgttg ccagcaatat gtttatgcca cagcttaaca
     2281 ggtggcgcag ggtttggacc tatttgccaa acgactatta tacttcaaat cgttactatc
     2341 cggtagtgta catgcacgat ggccaaaatg tatttgatgc ttttacatcg tttgcgggcg
     2401 agtggagtgt atacgatacg ttgcgaaata tga
//
LOCUS       DAOMAA010000247         2425 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_39228_length_2425_cov_2.244304, whole genome shotgun sequence.
ACCESSION   DAOMAA010000247 DAOMAA010000000
VERSION     DAOMAA010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39228_length_2425_cov_2.244304"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2425)
                     /locus_tag="PLO59_04190"
     CDS             complement(<1..>2425)
                     /locus_tag="PLO59_04190"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HQV00334.1"
                     /translation="TYNVAGNYQVTLTVYATNGDSAICIKNVTVVPLPQADFTMSNDT
                     VCQFETVFFNDASTSVNDSIVDWLWFYDTAGAVTTQAQYAFPTSGNFQVLLMVTNSNG
                     CTDSITKTVVVQPGPLSNFNVLNTCLGNAVLFTNTTFTAGLNATYSWSFGDGNQSTDI
                     NPQHAFAWVDSSYFISLTTQTSNGCVHTYFDSIAITQPANISISLSADTICKNESVTI
                     INSSTYSGNAYPVKTEWYFSDGSVFTNVDTLQHAFTQDGVQSVMLLITTPTACDTVIT
                     KSIYVLPNPTASFTTTQVCLGDSTLLASTAFAPSGDFITAHSWLLGNGMQATDSITKV
                     LYDSAAAYQVWHTVSTANGCTDSTMGYANVYQLPVANFSIPVAVCNDSLTSFIDFSLM
                     PTDTIVNWNWNFGNGITDSTKNGQVQFQNAGNYNVQLTVTSSKGCIGDTVKAVTVKPS
                     PMPAFVTANNCFNDYMQFSFVNMAPNQGLINQWQWNFGEQGALSVLENPLHKYAAPGT
                     FIVTVTVTDTNLCFASVQQNVVVNALPNVGFTVAQPCNQTSTIFTDTSIANGIAGLEW
                     NIQGQTFTTNTVNFLFDNAGTYPIVLTIVSDSSCTNTLQQNVVIHNMPQASFTTQPLY
                     AMPMQTVNFTNSSLFATHYEWSFGNGLSSTDLNPTTVYNDTGNYLNQLIAKNDYGCAD
                     TIQQVYNILYPRFEVALTDVYYSITNNLLNVSSRLINLGNLPVAEYDMVLRVDGLPEL
                     FEMSNKTLPVGGQPVVYDFKSQIALTDFFDATYFCAEISLPDGMVDDQINNKKCSALD
                     AKWQILQIWP"
BASE COUNT          729 a          478 c          460 g          758 t
ORIGIN      
        1 tgggccaaat ttgaagtatc tgccattttg catctaaagc actgcatttt ttattgttta
       61 tttgatcatc aaccatgccg tcaggtaaac ttatttcagc acaaaaataa gttgcatcaa
      121 aaaaatcggt aagtgctatt tgacttttaa aatcatacac aacaggctgc ccccctaccg
      181 gcaaagtttt gtttgacatt tcaaatagct cgggcaaacc atccactctt aaaaccatat
      241 cgtattcagc aaccggcaaa ttacccaggt ttattaacct gctgcttaca ttgagcagat
      301 tattagttat tgagtagtaa acatccgtta aagcaacttc gaaacgcggg tataaaatat
      361 tatatacctg ttgtatcgta tcggcacaac cataatcatt tttagcgatg agctgattta
      421 ggtaattacc ggtatcgtta tataccgtag tggggtttaa atcggtactg cttagtccat
      481 taccaaaact ccattcatag tgtgttgcaa ataaacttga gttggtaaag tttacagttt
      541 gcataggcat tgcatataat ggttgtgttg taaaacttgc ttgcggcatg ttatgtatta
      601 ccacattttg ctgtaaggta tttgtgcagc tactatcgct tacaatggtt aatacaattg
      661 gatatgtacc agcattatca aataaaaagt taactgtatt ggttgtaaag gtttggcctt
      721 gaatattcca ctccaaacct gcaattccgt ttgcaattga tgtatcggta aaaattgttg
      781 acgtttgatt gcagggttgt gcaactgtaa accctacatt gggcaatgca ttaaccacaa
      841 cattttgttg aacggatgca aagcacaggt tggtatcagt tactgtaaca gttactataa
      901 atgtaccggg ggctgcatat ttatgaagcg gattttctaa aaccgaaagc gcaccctgtt
      961 caccaaaatt ccattgccat tggttaatta agccctggtt tggtgccata tttacaaatg
     1021 aaaattgcat atagtcatta aaacaattgt ttgctgtaac aaatgccggc atgggcgatg
     1081 gttttaccgt taccgctttt actgtatcgc ctatgcaacc cttgcttgaa gtaaccgtta
     1141 gctgtacatt gtaattgcct gcattttgaa attgcacctg gccatttttt gtcgaatcgg
     1201 taataccatt accaaaattc caattccaat tcacaatggt gtcggttggc attaaactaa
     1261 aatctataaa gcttgtaagg ctgtcgttac atactgccac cggaatacta aaatttgcaa
     1321 ccggcaactg atacacattt gcataaccca ttgtagaatc cgtacatccg tttgcagttg
     1381 aaacagtatg ccaaacctga tatgctgcag cactatcgta tagcaccttt gttatactat
     1441 cggttgcttg cataccatta cccagtagcc agctatgtgc tgtaataaaa tcgcctgacg
     1501 gggcaaatgc agtacttgct aaaagtgtac tatcgcccag gcaaacttgt gtggtagtaa
     1561 atgatgctgt tggatttggt agcacgtata tggatttagt aataacagta tcgcaggcag
     1621 ttggtgtggt tataagcagc ataaccgatt gcacaccgtc ttgtgtaaat gcatgttgta
     1681 acgtatccac attggtaaat acactgccat cgctaaaata ccactcggtt tttacaggat
     1741 aagcattgcc actataagtg ctgctgttaa ttatggttac actttcattt ttacaaatag
     1801 tatcagcgct taaactgatg ctaatgtttg ccggttgtgt aatagctatt gaatcgaaat
     1861 aagtatgcac acaaccattg cttgtttgtg tagttagcga aataaaataa ctgctatcaa
     1921 cccaagcaaa agcatgttgc ggatttatat cggtgctttg attgccatcg ccaaaactcc
     1981 aactataagt ggcattaagg cctgcagtaa aagtagtatt ggtaaataac acggcattac
     2041 ccaggcaggt atttaaaaca ttgaaattgc taagtggtcc gggttgcacc acaaccgttt
     2101 tagttatcga atcggtacag ccattactgt tggttaccat taataatact tgaaaattac
     2161 ctgatgttgg aaaagcatat tgtgcctgtg tcgtaaccgc accggcagta tcgtaaaacc
     2221 acaaccaatc aacaatacta tcatttacac tggttgatgc atcgttaaaa aaaaccgttt
     2281 cgaactggca caccgtatcg ttactcattg taaaatcggc ttgcggcaat gggacaaccg
     2341 ttacattttt tatacatata gcactatcgc catttgttgc atatacagta agtgtaacct
     2401 gataatttcc tgcaacatta tacgt
//
LOCUS       DAOMAA010000248         2424 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_39256_length_2424_cov_2.420008, whole genome shotgun sequence.
ACCESSION   DAOMAA010000248 DAOMAA010000000
VERSION     DAOMAA010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39256_length_2424_cov_2.420008"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="PLO59_04195"
     CDS             <1..222
                     /locus_tag="PLO59_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007133455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HQV00335.1"
                     /translation="IKFAEWISKQLHKPGNLIFVAGSAYGFDAALVKDSPSISLSSFT
                     FTHQMVRLIFVEQLYRAFTIIKNEKYHHE"
     gene            complement(223..1437)
                     /locus_tag="PLO59_04200"
     CDS             complement(223..1437)
                     /locus_tag="PLO59_04200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQV00336.1"
                     /translation="MNVLYLSYDGMTDPLGQSQVLPYLFGLSKAGYKIHLISFEKPER
                     NHLVSGLKKEITEQGITWHPLVYHKKPAVLSTLFDILILKQKVIKLHTEFNFQLVHCR
                     SYITAIAGLHLKQKRGVSFIFDMRGFYADERVDGGLWPQDKFVYKFIYQYFKQLEKKL
                     LINADAIVSLTNSAKNIMQNWQLKKLPLPITVIPCCADLAHFNFMNSQKTITKTQLKI
                     ADDALVITYLGAIGTWYMLDEMLLFFKSLLQVKPDALFLFITTESANTIFNAAQKLDI
                     SQTQIRVVSANRQQVPSYLLLADLAIFFIKPTFSKKASSPTKQAELMGMGLPIICNAG
                     VGDTDYIVNETNSGIALPDLTVETFKKTADNLNELLCLDKHHIYEGAIRFFDLNKGIE
                     QYQQVYQRLLKQ"
     gene            1541..2146
                     /locus_tag="PLO59_04205"
     CDS             1541..2146
                     /locus_tag="PLO59_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00337.1"
                     /translation="MRVFEMISWYDLVIAPFYLLLIFFLARAIQKRNIDTQPIYRFYT
                     KALFFKVIGGICVCLIYTNYYYGGDTTNFFSDAILLNKVLFRDPGGYFYLMTHSVDEI
                     DYTLYDANIGYPIYVRDSSAYFIVKLTSVFVFFGFRSYIVATVLMAWVSFFGVWKLYR
                     VFADSFPSLSSQFAVGVFFMPSVFFWGSGILKDTVTFSAIL"
     gene            2203..>2424
                     /locus_tag="PLO59_04210"
     CDS             2203..>2424
                     /locus_tag="PLO59_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00338.1"
                     /translation="MLFVSNIMLIIRPYLLIGLLPGCFLWIIMAYSERVTGTMKKALV
                     IPVFVGMIVGLGYLMLFLLQESLGQYSADN"
BASE COUNT          701 a          424 c          451 g          848 t
ORIGIN      
        1 ataaagtttg ccgagtggat tagcaaacaa ttacataaac cgggcaattt gatttttgtt
       61 gccggcagtg cttatggttt cgatgcagca ttagtaaaag attcgccaag catcagctta
      121 tcatcgttta ctttcacaca tcaaatggtg cgcttaatat ttgtcgaaca attgtaccgc
      181 gcatttacaa taatcaaaaa cgaaaaatac catcacgaat aattattgtt ttagcaagcg
      241 ctgataaact tgctgatatt gttcaattcc tttattcaga tcaaaaaacc gtattgcgcc
      301 ttcgtaaata tggtgcttat ctaaacacag cagttcgttt aaattatcgg cagttttttt
      361 aaaagtttca acggttaaat cgggcaatgc aataccactg ttcgtttcgt ttacaatgta
      421 atctgtatcg cccacaccgg cattacatat tatgggtaat cccatgccca ttaactcggc
      481 ttgcttggtt ggcgatgagg cctttttcga aaaagtgggt ttgataaaga aaatagctaa
      541 atcggccaat aacaggtagg agggaacctg ctgacgattt gctgatacaa ctctgatttg
      601 ggtttgacta atatccaact tttgtgcggc attaaaaatt gtattggcgc tttctgtggt
      661 tataaataaa aacaaagcat caggttttac ctgaagtaat gatttaaaaa acaaaagcat
      721 ctcatccaac atataccagg tgccaattgc tccaaggtag gttataacca atgcgtcatc
      781 agctatttta agctgcgttt tggttatcgt tttttggcta ttcatgaaat taaagtgggc
      841 taaatcggca cagcatggaa ttaccgttat tggcaatggc agctttttaa gttgccagtt
      901 ttgcataata tttttagccg aattagttaa tgaaacgatg gcatcggcat tgataagtaa
      961 ttttttttca agctgcttaa agtattgata tataaattta tatacaaatt tatcctgtgg
     1021 ccataaacca ccatcaacac gctcatcggc ataaaagcca cgcatatcaa aaataaatga
     1081 aacgcctctt ttttgtttca aatgcaaacc agctatagct gttatgtagc tacggcaatg
     1141 caccaattgg aaattaaatt ccgtatgcag ttttattact ttttgcttta gtattagtat
     1201 atcaaatagc gtacttaaaa cggcaggttt tttatgatac accaacgggt gccaggtaat
     1261 accttgctca gttatctctt tttttaaacc ggatactaaa tggttacgct ccggtttttc
     1321 gaacgatatc agatggattt tataacccgc tttgctaaga ccaaataagt atggaagcac
     1381 ttgcgattga cctaaagggt cggtcatgcc atcataagaa agatataata cgttcatgat
     1441 ttaaaacgca gccaaattat aaaacacgcg tggttttttg aattaagatt atataaagca
     1501 tttaaaaaat acttttacca agttttaaat gtaaaagcac atgcgggttt ttgaaatgat
     1561 tagttggtat gatttggtga tagcgccatt ttacctgctg cttatttttt ttctggcacg
     1621 cgccattcaa aaacgcaaca ttgatacaca accaatttat aggttttata ccaaggcttt
     1681 gttttttaaa gtaataggtg gcatttgtgt ttgccttatt tataccaatt actactatgg
     1741 tggcgataca acaaactttt tttcagacgc catcttgctt aacaaggttt tgtttcgcga
     1801 ccctggcggt tacttctatt taatgacaca cagtgttgat gaaattgatt acactttata
     1861 cgatgcaaat attggctacc ccatttatgt gcgcgactcc agtgcttact ttattgtaaa
     1921 actcacatcg gtatttgtat tttttggatt cagaagttat attgttgcaa ctgtactaat
     1981 ggcttgggtt tctttttttg gtgtgtggaa actttaccgg gtatttgccg attcttttcc
     2041 atcattgagt tcacaatttg ccgtaggtgt tttttttatg ccatcggtgt ttttttgggg
     2101 gtcgggcatt ttaaaagata cggttacctt ttcagctatt ttgtaagttc ctttaatgca
     2161 ttatttatta gccgtaaaaa aatttcttcc aatattttaa tcatgctttt tgtgtccaat
     2221 attatgctca taatacgccc ctacttgctt attggtttat taccgggctg ttttctttgg
     2281 attataatgg cctatagcga acgtgttacg ggcacaatga aaaaggcttt ggtaataccg
     2341 gtttttgttg ggatgattgt aggcttaggt tatttaatgc tgtttttatt acaggaatct
     2401 ttaggccagt acagtgccga taat
//
LOCUS       DAOMAA010000249         2421 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_39350_length_2421_cov_2.439138, whole genome shotgun sequence.
ACCESSION   DAOMAA010000249 DAOMAA010000000
VERSION     DAOMAA010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39350_length_2421_cov_2.439138"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1392)
                     /locus_tag="PLO59_04215"
     CDS             complement(<1..1392)
                     /locus_tag="PLO59_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HQV00339.1"
                     /translation="MSNSLFKKKSITALLQEAASQGEGSLKRTLGAGNLVALGIGAII
                     GAGLFVRTAAASAEAAGPAVTLSFIVAAIGCAFAGLCYAEFASMMPIAGSAYAYSYVT
                     MGEIVAWIIGWALIMEYALGAATVSIAWSEYLNNLLGGAIPYEWCHSPFESLYTVTGD
                     AINSIVSIIPDAAKNIKNGTLIITDDQYHLIPADMLSLVSVSTGIFNAPALAILLLLT
                     MLLIKGTQESAFVNMIIVFVKVAIVLLFIAIGWQFINPANHTPYLIPEGTLGHDGFFK
                     WGWGGVLGGAAIVFFAFIGFDAVSTAAQEAKNPKRDMPIGILGSLVVCTILYILFGHV
                     LTGVANYNEFKTAGKEASVAYAIQTYMPGYGWLAKSVTIAILAGFSSVILVMLMGQSR
                     VFYTMSKDGLIPSVFGELHPKFKTPYKSNWILFVMVGIFAAFVPGSVAGDLTSFGTLF
                     AFVLVSAGVYILRV"
     gene            complement(1575..>2421)
                     /locus_tag="PLO59_04220"
     CDS             complement(1575..>2421)
                     /locus_tag="PLO59_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HQV00340.1"
                     /translation="IIINQAQNFGLSDLHQMRGRVGRSNKKAFCYLLTPPITVLTGEA
                     RQRLKAIEEFSDLGSGINIAMRDLDIRGAGNLLGGEQSGFISEIGFETFHKILDEAIF
                     ELKHGEFKDVFEDENKTDVNTIYVRDCQIDTDLEILFPSAYISNITERLNLYKELDDI
                     EAEDALLAFEQMLRDRFGPVPQPAIELMNTIRLRWKAKILGLEKIVLKNNLLKGYFTT
                     TGETAFFNSDIFTTILEFAKANPTKCKLKEIDGKSTITFFNVTTIEKANKQFDQLTGA
                     VVQNK"
BASE COUNT          759 a          494 c          418 g          750 t
ORIGIN      
        1 tactcttagt atatatacac cggcactaac caatacaaat gcaaacaaag taccgaatga
       61 ggttaaatcg cctgccacgc ttccgggaac aaaggctgca aatatgccca ccataacaaa
      121 taaaatccag tttgatttat aaggtgtttt aaattttgga tgcaattcgc caaataccga
      181 tggtattaaa ccatctttac tcattgtata aaacacacgg ctttgtccca tcaacataac
      241 taaaattaca ctgctaaagc cggccaaaat ggctatggta acacttttag caagccaacc
      301 gtatccgggc atataggttt gtatggcata agctacactg gcttccttac ctgccgtttt
      361 aaattcgttg taattggcaa cacctgtaag tacatgacca aataaaatat acagtatggt
      421 acaaaccact aacgacccta atatacctat tggcatatcg cgctttgggt ttttggcttc
      481 ttgtgctgca gtacttactg catcaaaacc aataaatgca aaaaacacaa tggctgcacc
      541 acctaaaaca ccaccccaac cccatttaaa aaatccatca tgccctaaag tgccttcggg
      601 tattaagtat ggtgtatgat tagccgggtt aataaattgc cagccaatag ctataaataa
      661 tagtacaata gccactttta caaacacaat tatcatgttt acaaatgccg attcttgtgt
      721 gcctttaatt agtaacatgg ttagtaacaa taaaattgcc aaagcaggtg cattaaaaat
      781 accggtgctt acactaacca agctaagcat atcggctggt attaaatgat attgatcatc
      841 cgttattatt agtgttccat ttttaatgtt tttagcagca tcgggtataa tgctaactat
      901 tgaatttatg gcatcacctg ttactgtgta taacgattcg aagggcgagt ggcaccattc
      961 gtaaggtatt gcccccccca gcaaattgtt taaatattca ctccaggcaa tacttactgt
     1021 tgctgctcct aaagcatatt ccataatcaa agcccaacca ataatccagg ctacaatttc
     1081 gcccatggta acataactgt aggcataggc gctacctgca ataggcatca tacttgcaaa
     1141 ctcggcataa cacaaaccgg caaatgcaca accaattgca gccacaataa atgataacgt
     1201 aacggcaggc cctgcagctt cggctgatgc agctgctgtg cgcacaaata agcctgcacc
     1261 aattatggca ccaataccta atgccaccaa attgccggca cctaaggtac gttttaaact
     1321 gccttcgccc tggctggcag cttcttgcag caatgctgta attgattttt tcttgaataa
     1381 tgaattcgac attgtggaaa taaatttgtg ttgagttatg tgagggttga cggttttcaa
     1441 acctacaagt taaaattgaa gttgcaaatc aatttagttg tatctataaa aattgtcacc
     1501 taaataaata attattgtaa ttacacttgg gtggacaatt attactaata ataaaaacta
     1561 aaaaatattt tctattactt attttgtaca acagcaccgg taagttggtc gaattgttta
     1621 ttggcttttt cgatggtggt tacattaaaa aaagtgattg ttgatttacc atctatctct
     1681 tttagtttgc atttagttgg attggctttt gcaaattcga gtatggttgt aaaaatatcg
     1741 gagttaaaaa atgctgtttc gcctgtggtt gtaaaatagc cttttagtaa attgtttttt
     1801 aatacaattt tttcaaggcc taatattttg gctttccatc taaggcgtat ggtattcata
     1861 agttcaatag ccggttgggg tacagggcca aacctatcac gtaacatttg ttcaaacgca
     1921 agcaatgcat cttcagcttc tatatcatct aactctttat acaagtttaa tcgctcggta
     1981 atattactga tgtaggccga tggaaataat atttcaagat cggtatcaat ttggcagtcg
     2041 cgtacatata tggtgtttac atcggttttg ttttcatctt caaacacatc tttaaactcg
     2101 ccatgtttta gttcaaaaat ggcttcatct aatattttat gaaatgtttc gaaaccaatt
     2161 tcagatataa atccgctttg ctcgccacct aataaattgc cggcaccacg tatatccaag
     2221 tcgcgcatgg caatattaat accactgccc aaatcgctaa attcttcaat ggcttttagt
     2281 cgttggcgtg cttcaccggt aagtacggtt atgggtggtg ttagcaaata acaaaaagcc
     2341 tttttattac tacggccaac acgtccgcgc atttgatgta aatcgcttaa accaaaattt
     2401 tgagcctgat taattataat g
//
LOCUS       DAOMAA010000250         2420 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_39368_length_2420_cov_2.794926, whole genome shotgun sequence.
ACCESSION   DAOMAA010000250 DAOMAA010000000
VERSION     DAOMAA010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39368_length_2420_cov_2.794926"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1135)
                     /locus_tag="PLO59_04225"
     CDS             complement(<1..1135)
                     /locus_tag="PLO59_04225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HQV00341.1"
                     /translation="MQSLELRNLFNQLPIGIIVLCMDNQVIFVNHTVCQIFNLTEMDE
                     PVQTDWINNLIVQCNLQLHINELKKGAYKSQLNVECNFNRSWNTNKKLNGVYFYEVEL
                     FKQFLIERDCIIISFNDITPFVNDAKAIRESHLNLKTIINNTRYNIFSVDANLRFIEF
                     NIPFKEEFDAMYQWDLKVGDSAVGHPIPPQTIADWIGFYGKAMGGKKIIQTYHFNANP
                     YMITLNPIFDDGKVVGIAVFSENLAAQQQLIGELEESKFRYSFAMDVSNSGYWDWNLE
                     TNHVYFSKIWKTMLGYQPTEIVNRYQSWENLLHKDDLDYVLDAVRKHLAGITETYITE
                     SRLLTKSGIYKWILAKGRVVEFNNEGKPKRFIGVHIDIDHIKSA"
     gene            1328..>2420
                     /locus_tag="PLO59_04230"
     CDS             1328..>2420
                     /locus_tag="PLO59_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008506770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HQV00342.1"
                     /translation="MTDNTYLGNGDVAAFDQMYQTYLKQPELLDLSWKKFFEGFDFAQ
                     TEFAPLIKSTQPANQLVVGKEFAVLNLINAYRIRGHLFTKTNPVRERRHYTPNLALEN
                     FGLTADDLATVFQAGSEIGLANATLKNIVEHLEQTYCHSVGAEFKYIRQPHVNKWLEE
                     KMEGCKNSYPFTIADKKHMLQKLNEAVAFENFLHTKFVGQKRFSLEGAETTIPALDAV
                     IEWGADMGIKEFVIGMAHRGRLNVLANILKKSYEDIFSEFEGKEFEISFDGDVKYHLG
                     YSTDVVTSNGKKVHLSLTPNPSHLEAVDPIVQGLVRSKIDTQNGSENEIAPILIHGDA
                     AIAAQGVVYEVVQMSLLEGYRTGGTVHLII"
BASE COUNT          764 a          476 c          441 g          739 t
ORIGIN      
        1 cggctgattt aatatggtca atatctatat gcacaccaat aaaacgctta ggcttacctt
       61 cattattaaa ttcaactaca cggccttttg ccaatatcca tttatagatg cccgatttgg
      121 taagcagccg gctttcggtt atatacgttt cggtaatgcc tgccaggtgt ttgcgcactg
      181 catctaatac gtaatccaaa tcatctttat gtagtaagtt ttcccaactt tggtagcggt
      241 ttacaatttc tgttggctga tagccaagca tggtcttcca tattttcgaa aaataaacat
      301 ggttggtttc caaattccaa tcccagtaac ccgaattact tacatccatt gcaaaactat
      361 accggaactt gctttcttct aactcgccaa taagctgttg ttgtgctgct aaattttcgc
      421 taaacacagc aatgccaacc actttgccat catcaaaaat agggtttaag gttatcatat
      481 aagggttggc attgaaatga tacgtttgta tgattttttt accccccatt gctttaccat
      541 aaaagccaat ccagtcggct atggtttgag gcggaattgg gtggcctacc gcactatcgc
      601 ccacctttaa atcccactga tacatggcat caaactcttc tttaaacgga atattaaatt
      661 cgataaaacg cagattggca tccacactaa aaatattata gcgtgtattg tttataatcg
      721 tttttaggtt taaatgactt tcgcgtatgg ccttggcatc attaacaaaa ggcgttatat
      781 cattaaacga aataattata caatcgcgtt caattaaaaa ttgcttaaac aactctactt
      841 cataaaaata aacgccatta agttttttat tggtattcca gctgcggtta aaattacact
      901 caacatttag ttggcttttg tatgcaccct tttttagttc gttgatatgc agttgcaggt
      961 tacactgtac aattaagtta ttaatccaat ctgtttgcac gggctcatcc atctcggtta
     1021 ggttgaatat ttgacaaacc gtatggttaa caaaaattac ctgattatcc atgcatagta
     1081 caattatacc aataggcaat tggttaaaca gatttcttaa ctccaatgat tgcatctgat
     1141 tttttagaaa ttgaatattt taaatcgaaa agcgaataaa caaacaatat aatgctgccc
     1201 aaaaagccgg ttggacaatt gcaataaaaa gtttacttaa aattagcgat accgcaacat
     1261 tattgcttca accaaatttt aatgtgcatc acattatact aatttcgcgc ctttaatttt
     1321 gacgttaatg actgataata cttatttagg aaatggcgat gtggctgcat ttgaccaaat
     1381 gtaccaaacc tatttaaagc aacccgaatt actggacctt tcgtggaaaa aatttttcga
     1441 aggttttgat tttgcacaaa ccgagtttgc tcctttaatt aaatcaacac aacccgccaa
     1501 tcaattggtt gttggtaaag agtttgcggt gttaaacctt ataaacgcat atcgcatacg
     1561 cggccattta tttaccaaaa caaatcccgt acgcgaacgc agacattata cacccaacct
     1621 agcactcgaa aactttggcc ttacggctga tgatttagca acggtgtttc aggccggcag
     1681 cgaaattgga ttggccaatg ccaccttaaa aaacattgtc gaacatttag aacaaaccta
     1741 ttgccatagt gtgggtgctg agtttaaata catcagacaa ccacatgtaa ataaatggtt
     1801 ggaagaaaaa atggaaggtt gcaaaaacag ctatccattt acaattgcag ataaaaagca
     1861 catgcttcaa aaactcaatg aggctgttgc tttcgaaaat tttctgcaca ctaaatttgt
     1921 tgggcaaaaa cgtttttcgc tcgaaggtgc cgaaactacc ataccggctt tagatgctgt
     1981 tattgagtgg ggggccgata tgggtattaa ggaatttgtt attggtatgg cacaccgtgg
     2041 cagattaaat gtgctggcca atattttaaa aaaatcgtat gaagatattt ttagtgagtt
     2101 tgaaggcaaa gagtttgaaa tatcatttga cggtgatgta aaatatcacc tgggctacag
     2161 taccgatgtt gttaccagca atggtaaaaa agtacacctg tcgctaacac ctaacccctc
     2221 acatctcgaa gcagttgatc cgattgtgca agggttggta cgttcaaaaa ttgacacaca
     2281 aaatggcagc gaaaatgaaa ttgcacccat actgatacat ggcgatgcag caattgctgc
     2341 acaaggcgtt gtgtatgaag tagtacaaat gagtttgctt gaaggctacc ggaccggtgg
     2401 caccgttcac cttatcataa
//
LOCUS       DAOMAA010000251         2415 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_39503_length_2415_cov_3.324153, whole genome shotgun sequence.
ACCESSION   DAOMAA010000251 DAOMAA010000000
VERSION     DAOMAA010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39503_length_2415_cov_3.324153"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..176)
                     /locus_tag="PLO59_04235"
     CDS             complement(<1..176)
                     /locus_tag="PLO59_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HQV00343.1"
                     /translation="MLQSYLHKHLIEAGCDEAGRGCLAGPVFAAAVILPPNTNHELLR
                     DSKKLNVQQRLTMR"
     gene            220..1665
                     /locus_tag="PLO59_04240"
     CDS             220..1665
                     /locus_tag="PLO59_04240"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HQV00344.1"
                     /translation="MQKKFITNIAFLVFVNILVKPFWILGIDRAVQNAVGTEHYGFYA
                     SLFNFSLLFNIFLDLGITNYNSRNISQNMQLVQKHFSGIVALRLLLAGLYLLIGLVTA
                     FIIGYQAYQFQLLIILFFNQILLSFIMYFRSNISGLHLFKTDSIISVIDRLIMILICG
                     FLLIFFPQEFSSHLEWFALSQTVGYLITAAITLWILHGKVTLQRITFHWPFLITILKK
                     SAPFAILIFLMGFYNRIDAVMIERLLPNNGAEQSGIYAQAYRLLDAANMIAFLFGGLL
                     LPIFASQLKNKISVEPLTKLSYCLISVPAVILICCCMFYKTQIMDLLYESHHNESAQV
                     LGVLVFCFFAMSSNYIFGTLLTANGNLKQMNIMALCGMCINIGLNLILIPKYFAFGAA
                     CSSLVTQFATAIIQIGLSHKVFGFKLDSKLLALFALYAVLVMAVSYLTSQLISNWLVG
                     ILLTSTIALGLAFLLKLINLKHIALIMKQEE"
     gene            1750..2397
                     /locus_tag="PLO59_04245"
     CDS             1750..2397
                     /locus_tag="PLO59_04245"
                     /inference="COORDINATES: protein motif:HMM:NF012720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HQV00345.1"
                     /translation="MKTDTSRMSTQNQGKRTEKISAFRRLEKMHPHKTFLFFAIIAST
                     LSFLALVLLYGIRMVEYQNVAFGFPKLFSVSTVVLLFSSYTMSRCITAFKTDSMIDLR
                     ISLFLTLLLAGVFCTMQVFCWQKMYADGVFFSYQTAVSFLYLISAFHLLHILLGFVML
                     AYLNFNTYVAANDMVKQLMFFSSKREGNKLEIVTMYWHFVDFMWLFLYFIFLFSF"
BASE COUNT          649 a          388 c          462 g          916 t
ORIGIN      
        1 tggcgcatgg ttaaacgttg ttgcacattt agttttttcg aatcgcgcag taattcgtga
       61 ttggtattag gcggcagtat aaccgctgcg gcaaataccg gccctgccaa acaaccacgg
      121 ccggcttcat cgcatccggc ttcaattaaa tgtttgtgca aataagattg cagcataggc
      181 tattcaattt tttatacgtt tgggcgctca attttagtga tgcaaaagaa atttataacc
      241 aacatagctt ttttggtttt tgtaaatatt ctggtaaaac cattttggat tttaggtatt
      301 gaccgtgccg tacaaaatgc agtaggtact gagcattatg gtttttatgc atcgcttttc
      361 aatttttcat tgctctttaa tattttttta gatctgggta ttaccaatta caatagtcgc
      421 aacatatcgc aaaatatgca attggtacaa aagcattttt caggcattgt ggctctaaga
      481 ttgttattgg caggtttgta tttgcttatt ggtttggtaa cagcttttat aattggttac
      541 caggcttatc agtttcagtt gctgataatt ctttttttta atcagatatt attatcgttt
      601 attatgtatt tcaggtcgaa tatatcgggc ctgcatttat ttaaaaccga tagtattata
      661 tcagttattg acagactgat aatgattttg atttgtggtt ttttgctcat cttttttcca
      721 caggagttta gcagtcattt agagtggttt gctttatcgc aaacggtcgg ttacctgatt
      781 actgcagcta ttacattatg gatattacat ggtaaagtaa ctttgcagcg cattactttt
      841 cattggcctt ttttaattac catattgaag aaaagtgcgc catttgctat actcattttt
      901 ttaatgggtt tttataaccg tattgatgca gttatgattg aacgtttgct gccaaacaac
      961 ggtgccgagc aaagtggtat atatgcccag gcatatcgtt tattggatgc ggccaatatg
     1021 attgcctttt tatttggagg tttattgttg cccatatttg catcgcagct taaaaataaa
     1081 atcagtgtcg aaccgcttac caaattaagt tattgcctca ttagtgtacc tgccgtaatt
     1141 ttaatttgct gttgtatgtt ttataaaacg cagataatgg acttgctgta tgaaagtcat
     1201 cacaatgagt cggcacaggt tttaggggtt ttggtatttt gtttttttgc tatgtcgagc
     1261 aactacattt ttggcacctt actaacagca aatggtaact taaaacaaat gaacataatg
     1321 gctttatgcg gtatgtgcat caacatcggg ctaaacttaa ttctcattcc taaatacttt
     1381 gcttttggtg ctgcctgcag tagcttggtt acacagtttg caacggccat tattcaaata
     1441 ggtttatcgc ataaagtttt tggctttaaa ctcgattcaa agttattggc tttgtttgct
     1501 ttgtatgctg tactagtaat ggccgtttct tacctaacat cgcaattaat aagcaactgg
     1561 ttggtaggta ttttattaac gtcaaccata gcgttgggct tggcattttt gcttaaactg
     1621 ataaatttaa aacacattgc tttgataatg aagcaagagg agtaagcgtt tcttatcgga
     1681 tttgtttaac taaaaaacta cgacataaac tttgaaatca tgttggttaa tttcaattaa
     1741 tcgttatttt tgaaaaccga cacttcacgt atgagtacac aaaaccaagg taagcgcacc
     1801 gaaaaaataa gtgcattcag acgtttagag aaaatgcacc cgcataaaac gtttttgttt
     1861 tttgcaatta ttgccagcac actaagtttt ttggcattag tgcttttgta cggcatacgc
     1921 atggtagagt atcaaaatgt tgcctttggt tttcctaagt tgttttcggt tagcactgta
     1981 gtacttttat ttagtagtta caccatgtcg cgctgcataa ctgcttttaa aaccgatagc
     2041 atgattgatt tacgaatcag tttatttctt acgcttttat tggcaggtgt tttttgtaca
     2101 atgcaggttt tttgttggca aaaaatgtat gccgatggtg tgtttttcag ctatcaaaca
     2161 gcggtgtcat ttttatatct aatttctgcc tttcatttac tgcatatttt attaggtttt
     2221 gttatgctgg cctatttaaa ctttaatacc tatgtagccg ctaatgatat ggttaagcag
     2281 ttgatgtttt ttagcagtaa acgcgagggc aataaattgg aaatagtaac catgtactgg
     2341 cattttgttg attttatgtg gctgttttta tattttattt tcttgttttc tttttaatga
     2401 aaacagttgc tattc
//
LOCUS       DAOMAA010000252         2409 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_39692_length_2409_cov_2.818607, whole genome shotgun sequence.
ACCESSION   DAOMAA010000252 DAOMAA010000000
VERSION     DAOMAA010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39692_length_2409_cov_2.818607"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1457
                     /locus_tag="PLO59_04250"
     CDS             <1..1457
                     /locus_tag="PLO59_04250"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HQV00346.1"
                     /translation="IGFTNDGGRTDIAANGTFLNNSMMPSLGYNEGVELSDEDDRIEY
                     KLPKKPYRMRPITDTASYANTYLAQDADWVQYKCTLSTTADQIAISPGYLQNEWMQNG
                     RKYYAYAMDAPILNFFSYLSARYSVNRDVWSNPKDANQKVNIEIFSHPTHQWNVAGMK
                     NAIKKTLDYASANFSNYQFRQVRILEFPRYRNFAQSFPNTIPFGEGLGFILDVDKEND
                     IDMAFYVTAHEVAHQWWGHQVAGANCQGSTVMVETMAQYTALMVMEHEFGTAHMEKFL
                     KYEMDDYLRGRSAERQKELPLMLCENQGYIHYNKGSCIMYALKDYVGETKLNKALQQF
                     IKTYQFQNPPYVTAPVFVDYLKQATPDSLKYIITDMFENITVFNLRTTAVTALKTNNT
                     YRVNVNIDALKAVADSVGNEQNIKLNDWVDIGVYTQTKSGNDSLIYLQKHLVANNTKQ
                     VTVTVNQLPSKAGIDPLHKLIDRQTSDNVKKITL"
     gene            1603..2394
                     /locus_tag="PLO59_04255"
     CDS             1603..2394
                     /locus_tag="PLO59_04255"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HQV00347.1"
                     /translation="MNVMQHVVLIKSLHLKTAVKTDIIVPDNLQSTNTYPLLVLNDGQ
                     DMEVMQMPHLLKQVWTKNVCRPFILAAPHANNRLEKYGVANHPDFKNRGKLAKAYTLF
                     VIEELIPFVQTFLKQYGFSEHVMAGFSLGGLSAFDIGWNNAKIFSKVGVCSGSFWWRN
                     KDLQKGYTEANRIMHKVVRLTEDKPSIKIWLQCGTLDETADRNNNGIIDSIDDTLDLI
                     IELEAKGFIKNRDLFYNELIGGKHTLETYGIVLPYFFQWAFGRPA"
BASE COUNT          817 a          445 c          482 g          665 t
ORIGIN      
        1 aaataggctt taccaatgat ggtggccgta cagatattgc tgcaaatggc actttcttaa
       61 acaacagcat gatgccatcg ctcggttata atgagggtgt agagttaagc gatgaagacg
      121 acagaattga atataaactg cctaaaaaac cctaccgtat gcgacctatt accgatacgg
      181 caagctatgc caacacttac ctggcgcaag atgccgattg ggtacaatac aaatgcacct
      241 taagcacaac ggccgatcag attgccatat cgcccgggta cctgcaaaac gaatggatgc
      301 aaaatggccg taaatattat gcctatgcta tggatgcgcc tattctaaat tttttcagct
      361 atctaagtgc gcgttatagt gtaaaccgcg atgtgtggag caatccaaaa gacgcaaatc
      421 aaaaagtaaa catcgaaata tttagtcatc ccacacatca atggaatgtt gccggaatga
      481 aaaatgccat taaaaaaacg cttgattatg catcggcaaa cttttctaac taccaattca
      541 ggcaagtgcg tattttggag tttccacgat atcgaaattt tgcacagtcg tttccaaaca
      601 ccataccctt tggcgaaggc ttgggtttta ttttagatgt agataaggaa aacgatattg
      661 acatggcttt ttatgtaaca gcgcatgaag tagcccatca atggtggggc catcaggtgg
      721 caggtgccaa ttgccagggc agcactgtta tggttgaaac gatggcccaa tacacagccc
      781 ttatggtaat ggaacatgaa tttggtacgg cacacatgga aaaattttta aagtatgaaa
      841 tggatgatta cctgcgcgga cgcagtgccg aaaggcaaaa ggaactgcca ttaatgcttt
      901 gcgaaaatca gggatacata cattacaata aaggcagttg cattatgtat gctcttaaag
      961 attatgttgg cgaaacaaaa ttaaacaagg ctttgcaaca gtttattaaa acctatcaat
     1021 ttcaaaaccc gccttatgta acagcgcccg tatttgttga ttatttaaaa caagcaacac
     1081 ccgatagttt aaaatatatt attactgata tgttcgaaaa cattacggtt tttaatttgc
     1141 gtactacagc tgttacagcc ctaaaaacta ataacacata tagggtaaat gtaaatattg
     1201 atgcgctaaa ggcagtagcc gatagtgtgg gtaatgagca aaatattaag ctcaacgatt
     1261 gggtggatat tggtgtttac acacaaacca aaagcggtaa cgattcgctt atttatcttc
     1321 agaaacacct tgttgctaac aataccaaac aggtaactgt taccgtaaac caattgccga
     1381 gtaaggcagg tatcgaccca ttacataagc ttatcgacag gcaaacatct gataacgtta
     1441 agaaaattac cctctgataa atacagaaat ggtttaccac ttaagtgaat gttttttata
     1501 aatgttggct cgtatgcttt acaattcgaa cgttgataag ttcattttaa gacaaaaata
     1561 agtatgtacg tattttaaag taagtggcta acattgccgg ccatgaatgt aatgcagcat
     1621 gttgtcctaa ttaagtcatt gcacttaaaa acggcagtaa aaacagatat aatcgttccc
     1681 gataatttac aaagcaccaa tacctatcca ttattagttt taaacgatgg gcaggatatg
     1741 gaggtaatgc aaatgccaca tttattaaag caggtgtgga caaaaaatgt atgcaggcca
     1801 tttatattag ctgcaccaca tgcaaacaac agactcgaaa aatatggcgt ggccaatcac
     1861 cccgacttta aaaaccgcgg caaacttgcc aaggcataca ccttgtttgt tatagaagaa
     1921 ctcatacctt ttgtacaaac ttttttaaaa caatatgggt ttagcgaaca tgtaatggcc
     1981 ggctttagtt taggtggatt atcggctttt gatattggtt ggaacaacgc caaaattttt
     2041 agtaaagtag gggtatgcag tggctctttt tggtggcgta ataaagacct tcagaaagga
     2101 tataccgaag ccaatagaat tatgcataaa gtagtaaggc taaccgaaga taaaccatcc
     2161 attaaaatat ggctgcaatg cggtacctta gacgaaactg ctgatagaaa caacaatggc
     2221 attattgata gtattgacga taccctcgat ttaattattg aattagaagc caaaggattt
     2281 attaaaaaca gagacttatt ttacaacgaa ctaattggcg gcaagcacac tttagaaacg
     2341 tacggtattg tactgccata cttttttcaa tgggcttttg gcaggcctgc ttagtttatg
     2401 ataaaatat
//
LOCUS       DAOMAA010000253         2408 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_39734_length_2408_cov_2.351041, whole genome shotgun sequence.
ACCESSION   DAOMAA010000253 DAOMAA010000000
VERSION     DAOMAA010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39734_length_2408_cov_2.351041"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="PLO59_04260"
     CDS             <1..714
                     /locus_tag="PLO59_04260"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV00348.1"
                     /translation="YAYDDGTAEAGYSITNAVNAKIAMRFDLLQADTVRAVQIFFVQQ
                     YDNSALNTFRLAIWSSLTPENLIYQRSSLLPIYTDSINGFATYAIPDTTLILQGTVYI
                     GLICNLAKDYDLGFDFNTNNQNKMFFNSNGNWVNTTFQGSFMIRPIFGDSAIYAGVDD
                     VAAKHQITLYPNPTNNSININSNLNFTQAVIYNTLGKQVVIIPVVEKTIHVESLNAGM
                     YYVQFRTKEGIITRKFIKN"
     gene            762..1790
                     /locus_tag="PLO59_04265"
     CDS             762..1790
                     /locus_tag="PLO59_04265"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012781380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HQV00349.1"
                     /translation="MNNVDTEQEQEELYEHHRIVVDKGQSLLRIDKFLGNRLENTSRN
                     KIQTAAATDCIRVNDKPVKPSYKVKPGDVITIVFSHPPRDIELYADAIPLNIVYEDDQ
                     VIVINKPAGMVVHPAYGNYRNTLVNALVHHFHPHLGNAPLKSETLRPGLVHRIDKNTS
                     GLMVVAKDEYSLSFLAKQFFDRTIERKYIALTWGTFEHDKGTITGYVGRNLKNRKIMD
                     VFTDEKFGKHAVTHYTVLERLGPVTLLQCKLETGRTHQIRVHMKYIKHPLFNDAEYGG
                     DAILKGTVYNKYKQFVQNCFDLLPRQALHAASLGFMHPITKKLLFFEAALPTDFDNAL
                     QKWRQYAD"
     gene            complement(1825..>2408)
                     /locus_tag="PLO59_04270"
     CDS             complement(1825..>2408)
                     /locus_tag="PLO59_04270"
                     /inference="COORDINATES: protein motif:HMM:NF021210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HQV00350.1"
                     /translation="QFVMHASNNELLGLNKFVHRTFNGQDALAFVDALRKAYNHKGLQ
                     ALFTPNYPDIRLQHAAYMHPLAAIIFNFRYAFTKSFKWQRSAKHIGNPYKNSSAKRMC
                     MFLRWMVRQDKNGVDFGIWRNINPADLICPLDVHSGNVARQLGLLTRPQNDWKAAVAL
                     TESLKQYNTNDPTIFDFALFGMGVNSKRLAVIN"
BASE COUNT          756 a          453 c          463 g          736 t
ORIGIN      
        1 tatgcttatg atgatggtac tgccgaagca ggctattcta ttaccaatgc tgttaatgca
       61 aaaatagcta tgcgcttcga tttactgcaa gccgataccg taagagctgt tcaaatattt
      121 tttgtacaac aatacgataa cagtgcttta aacactttca gattggcaat ttggagtagc
      181 ctaacgcctg aaaatttaat ttatcagcga agcagtttgt tacccattta taccgacagc
      241 ataaatggtt ttgccactta tgcaataccc gatactacat taattttaca aggcacggtt
      301 tacataggcc ttatttgcaa cctggcaaag gattatgatt tgggttttga ttttaacacc
      361 aacaatcaaa acaaaatgtt ttttaattcg aacggtaatt gggttaatac tacgtttcaa
      421 ggctcattta tgatacgccc catttttggc gattcggcaa tttatgccgg tgttgatgat
      481 gttgctgcaa agcatcaaat aacattgtat ccaaatccaa ccaataactc aattaacatt
      541 aatagtaatt tgaatttcac gcaagctgtt atttacaaca ccttgggcaa gcaagtagtc
      601 attatacctg ttgttgaaaa aacaatacat gttgaaagcc ttaatgctgg catgtactat
      661 gtacaatttc gaaccaaaga aggtataata acccgaaagt ttattaaaaa ttaattggtc
      721 agtatgcgcc aaaatgatgc attatatttt catttcgttt tatgaataat gtagataccg
      781 aacaagagca ggaagaactc tatgagcatc atcgcattgt agttgataag ggacagtcgc
      841 ttttgcgaat tgataaattt ttgggcaacc gattagaaaa tacatcacga aacaaaatac
      901 aaacagcagc cgctacagat tgcattcgtg ttaatgataa gccggtaaaa ccgagctata
      961 aagttaagcc gggcgatgta ataaccatcg tattttcgca tccaccacgc gatattgaat
     1021 tgtatgccga tgcaattccg ttaaacattg tttacgagga cgatcaggta attgttataa
     1081 ataaaccggc cggtatggtg gtgcatcccg cttatggtaa ttaccgcaat acgttggtta
     1141 atgctttggt acaccatttt catccgcatt taggtaatgc accattaaaa tcagaaacgc
     1201 tgcggccggg tttggtgcat cgtattgata aaaatacctc gggtttaatg gtagtggcaa
     1261 aggatgaata cagtttgtca tttttagcaa aacagttttt cgaccgtact atagaacgta
     1321 aatatattgc attgacctgg ggtacttttg agcatgataa aggaaccata accggatatg
     1381 taggccgtaa cttaaagaac cgtaaaataa tggatgtgtt taccgatgaa aaatttggta
     1441 aacatgctgt tacacattac accgtattag agcgtttagg ccctgttacc ctcttgcaat
     1501 gtaaattgga aacgggacgc acgcatcaaa tacgtgttca catgaaatac attaaacacc
     1561 cgctttttaa tgatgccgaa tacggtggcg atgccatttt aaaaggtacg gtttacaata
     1621 aatacaaaca gtttgtacaa aattgtttcg acttgttgcc caggcaggca ttgcatgctg
     1681 catcgttggg atttatgcac ccaattacca aaaagttact ttttttcgaa gcagcattgc
     1741 caaccgattt tgacaatgct ttgcaaaagt ggcggcagta tgccgattga ttttgtttca
     1801 gatacgataa caatcagcaa ctgattaatt aataactgcc aaccttttag aatttacacc
     1861 cataccaaat aatgcaaaat cgaaaattgt ggggtcgttg gtattgtatt gctttaagct
     1921 ttcagttaat gctacagcag ctttccagtc gttttgcggg cgtgtaagta ggcccaattg
     1981 gcgcgctaca ttgccactgt gaacatctaa cggacatatt aaatcagcag gattaatgtt
     2041 tctccaaatt ccaaaatcaa caccgttttt atcttgcctt accatccatc ttaaaaacat
     2101 gcacatgcgc ttggctgaac tatttttata aggatttcca atgtgtttgg cacttcgttg
     2161 ccatttaaac gattttgtaa acgcatacct gaaattaaaa ataatagcag ccagcggatg
     2221 catgtatgcc gcatgttgta atcgtatatc ggggtagtta ggcgtaaata gtgcctgcaa
     2281 acccttatgg ttgtatgctt tacgtaatgc atcaacaaat gccaaagcat cttgtccgtt
     2341 aaacgtgcgg tgtacaaatt tattaagccc aagcaattcg ttatttgatg catgcattac
     2401 aaattggt
//
LOCUS       DAOMAA010000254         2407 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_39759_length_2407_cov_2.775935, whole genome shotgun sequence.
ACCESSION   DAOMAA010000254 DAOMAA010000000
VERSION     DAOMAA010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39759_length_2407_cov_2.775935"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1102
                     /locus_tag="PLO59_04275"
     CDS             <1..1102
                     /locus_tag="PLO59_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HQV00351.1"
                     /translation="TEKYSRHPARYTEASLVKKLEELGIGRPSTYAPTISTVQQRGYV
                     VKEDRQGRERGYVFLSLKNNQITRQEKIENTGAEKNKLFPTDIGTVVTDFLVKNFDDV
                     LDYSFTARVEKEFDEIAEGQKKWQQMIGAFYQPFHQDVINTSETAERASGERTLGIDP
                     ISKKQVSVRIARFGPVAQLGEGTETEKPQYASLRAGQRLETITLDEALDLFKLPRIVG
                     RYLDKDIKIAIGRFGPYAQHNNVFYSLPKGEDPYTISYDSAVEVILAKQKADIEKVIK
                     VFSENEDAKVLKGRWGPYIAFGKKNIKIPKDRDPISISYAEVLELEKTTPESKPRGFF
                     KKAASKATPAVSKTPVKKVTKKSAPGKKASKK"
     gene            1149..2315
                     /locus_tag="PLO59_04280"
     CDS             1149..2315
                     /locus_tag="PLO59_04280"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HQV00352.1"
                     /translation="MNLKEFQNYLQEPTISQNFDTDCLGTKVEKVYESGVNDTLEGVS
                     LALIGVDEFRASQTNYNCGGNSDAIRSKLFDLMKHDSAGIIADLGNLKLGNTTQDTCT
                     AISNISAELIRNKIVPIIFGPSQDVTFAQYEAYKQLAQIINLVNVDSRFDLGTPNDDL
                     HAQSYVGKIILEQPNFLFNYSHIGYQTYYVGDNAVSQMSKLYFDTYRLGHVTTDIADM
                     EPVLRNSDMISFDLSAIKSADAKASLKPGPNGLSGAEACQLMYYAGMSEKLSSVGIYN
                     YVNQKDDAGLTAELAAQMIWYFIEAFFKRKNEMPQYNKNGFLKYTVPLHSTNHELIFL
                     KSTKSDRWWMEIPIADNYTKLQRHHIVPCSHNDYLMACSNEMPVRWWQAYQKLC"
BASE COUNT          854 a          417 c          470 g          666 t
ORIGIN      
        1 aacagaaaaa tattctcgtc atccagctag atatacagaa gcaagtttgg taaaaaagtt
       61 agaggaatta ggcattggcc ggccatcaac ttatgcccca actattagta cagttcaaca
      121 acgtggatat gtggtaaaag aagacaggca aggacgcgaa cgtggttatg tattcctttc
      181 acttaaaaat aatcaaatta cgcggcagga aaaaattgaa aataccggag ccgaaaaaaa
      241 taaattattc cctactgata taggtactgt tgtaaccgac tttctggtaa aaaattttga
      301 tgatgtatta gattattcat ttacagcacg cgtagaaaag gagtttgatg agattgccga
      361 aggacaaaaa aaatggcagc agatgattgg tgctttttac caaccatttc atcaagatgt
      421 aatcaataca tcagaaacag ccgaacgtgc ttcaggcgaa cgtactttag gcattgaccc
      481 aatatctaaa aaacaagtta gtgttcgtat agcgcgtttt gggcctgtgg ctcaattggg
      541 tgaaggtact gaaaccgaaa aacctcagta tgccagcttg cgtgccggcc agcgtttaga
      601 aaccattacg cttgatgaag cacttgattt gtttaaatta ccacgcattg ttggccgtta
      661 tttagataag gacataaaaa ttgcaatagg tagatttgga ccttatgcac aacacaataa
      721 tgtgttttat tcattgccaa agggcgaaga cccttataca atttcatacg atagcgcagt
      781 agaagtaatt ttagctaaac aaaaagccga catcgaaaaa gttattaaag tattctcaga
      841 aaatgaagat gctaaagtgc taaagggtag gtggggacct tacatagcat tcggtaagaa
      901 gaatattaaa atcccgaaag accgcgaccc catttcaata tcttatgccg aggtattgga
      961 gttagaaaaa acaacaccag aatcaaaacc gcgcggtttt tttaaaaaag ctgcatcaaa
     1021 agctacaccg gcagtttcga aaacacccgt taaaaaggtt accaaaaaat cagcaccggg
     1081 caaaaaagca agcaaaaagt aaacacacac aaactaagtt aatctaatta ttctatcagc
     1141 aatttctgat gaacttaaaa gagtttcaaa attatttgca ggagcctaca ataagtcaaa
     1201 actttgatac agattgttta ggcactaaag ttgaaaaagt atatgaaagc ggagttaacg
     1261 acacgcttga aggtgtaagc ttggcattga ttggtgttga tgaatttagg gcatcacaaa
     1321 ccaattataa ttgtgggggt aacagtgatg caatcagatc aaaactgttt gatttaatga
     1381 agcatgatag tgcgggaatt attgccgatt taggtaactt aaagttagga aacacaacgc
     1441 aagacacatg taccgctatt tcaaatataa gtgccgaact tatacgaaat aagattgtgc
     1501 caattatatt tggccctagc caagatgtaa ctttcgccca atatgaagct tacaaacaat
     1561 tggcgcaaat tattaatctg gtaaatgttg attcacgttt tgatttaggt acaccaaatg
     1621 atgacttaca tgcacaatcc tatgttggga aaataatttt agaacaacct aattttttat
     1681 ttaattatag tcacataggg tatcaaactt attatgttgg agataatgct gtaagtcaaa
     1741 tgtcgaaatt atattttgac acctatcgct taggccatgt aactactgat attgctgata
     1801 tggaaccggt gctgcgaaat tcagacatga ttagtttcga tttatcagca atcaagagtg
     1861 cagatgcaaa agcgtcctta aaaccagggc ctaatggact tagtggtgcc gaggcttgtc
     1921 agctcatgta ctatgcaggc atgagcgaaa aattaagctc cgtaggtatt tacaattatg
     1981 taaatcaaaa agatgatgct gggctaaccg ctgaattggc agcacaaatg atatggtatt
     2041 ttatcgaagc cttttttaaa cgaaaaaatg aaatgccgca atacaacaaa aatggatttt
     2101 taaaatatac ggtaccgctc catagtacca atcatgaatt gatttttctg aaaagcacaa
     2161 aaagtgatcg ttggtggatg gaaataccta ttgccgataa ttacacaaaa cttcaaagac
     2221 atcatatcgt accttgctcg cataacgatt acttaatggc ttgtagcaat gagatgccag
     2281 ttaggtggtg gcaagcctat caaaaacttt gttaacatta taaactgtaa aaaatgactt
     2341 cagcctaatt tgggtattga aaataatttt gctttctttc agccgtcaaa taccctcacc
     2401 caatttt
//
LOCUS       DAOMAA010000255         2407 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_39762_length_2407_cov_2.669218, whole genome shotgun sequence.
ACCESSION   DAOMAA010000255 DAOMAA010000000
VERSION     DAOMAA010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39762_length_2407_cov_2.669218"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /locus_tag="PLO59_04285"
     CDS             complement(<1..989)
                     /locus_tag="PLO59_04285"
                     /inference="COORDINATES: protein motif:HMM:NF019187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HQV00353.1"
                     /translation="MQNKGAVRLFAILLAIVCVYQLSFTIFTKSVENSAKEYAKGDVK
                     LESLYLDSMNNEEVYPLLVKNFTYAECKDREINLGLDLKGGMNVTLEVSVPEIVRAMG
                     NYSTDANFNKALETAISKERNSQLDFVTLFGNEFKAIAPNDKLAAIFSTVELQNKINY
                     NSTNDQVLAVLKTEVNESVDRSYNTIKTRIDKFGVTQPNVQKLANGRILVELPGVKEK
                     ERVRKLLQGTAKLEFWPTYNFGEVVKNLVEVNKVLKVSAERDSLLNKPASDSTSAAIA
                     VADTNVTKVDSAASGLLSKIDEAAKDTSKKDTTKTMTMEESLKEYPLFTYLLP"
     gene            1280..>2407
                     /locus_tag="PLO59_04290"
     CDS             1280..>2407
                     /locus_tag="PLO59_04290"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HQV00354.1"
                     /translation="MKKIFLFLSVALTYQFGNAQTKFVRDLNIPLTQYGLPAPLAYSG
                     GINGGMFSEIDLNNDGIKDLFLFDKDQVYKKARISTFLNTGTPNTVSYQYAPEYIANF
                     PTNLADWAILVDYNHDGKEDIFTYSNQIPAAGITVYKNISTPTQLQFALEYSLLYANI
                     PSGQGNIYVSSNDKPAIVDFDGDGDLDIIAIYIGGGRLEWSKNIGMETLGKNDTLIFD
                     WQPSCWGHFGFSANNNSAILNVACKPIAFVNNGDLPATLLTPEPTPDPTRHNGACFVI
                     EDFNKDGLVDYLGGDAIGNNILYLQNGGTLSDPLMISQDSLFPVYDNSAFFFEFLNPT
                     HIDVNNDGKKDLLVSPCKNGDAENFHNVWYYKNVGTVADSFS"
BASE COUNT          774 a          429 c          410 g          794 t
ORIGIN      
        1 cttggaagta aataagtaaa caaaggatac tctttcaatg attcttccat agtcattgtt
       61 ttagtagtat cttttttact cgtatcctta gcagcttcgt ctattttact tagcaaacca
      121 ctggcagcgc tgtcaacttt agtaacattt gtatcagcaa cggcaatggc agcggaagta
      181 ctatctgatg ctggcttatt taataatgaa tcgcgctctg ctgaaacttt taatacctta
      241 ttaacttcaa ctaaattttt tacaacctca ccaaaattgt aggtaggcca aaactctaac
      301 ttagcggtac cctgtaaaag tttacgtacg cgttcttttt cctttacgcc aggtaactca
      361 accaatatac gtccattagc taatttttga acattgggtt gcgtaactcc aaatttatca
      421 atacgtgttt taatcgtatt gtaagatctg tcaacgcttt cgtttacttc ggtttttaaa
      481 actgcaagta cttgatcgtt ggttgagttg taattaattt tgttttgcaa ctcaaccgta
      541 gaaaatatgg cagccaactt atcatttggt gcaatagctt taaactcatt accaaaaagt
      601 gttacaaaat caagttgtga attacgttcc tttgagatag cagtttcaag tgctttatta
      661 aaatttgcat ccgtactata attacccatg gcacgaacaa tttcaggaac agatacttca
      721 agtgttacgt tcataccacc ttttaagtct aggcctaaat taatctcacg atccttacac
      781 tctgcataag tgaaattttt aacaagtaat ggataaactt cctcattgtt catcgaatct
      841 aaataaaggg actctaattt cacgtcaccc tttgcatatt ctttggccga attttctaca
      901 cttttggtaa atatggtaaa cgacaactga tacacacaaa ctatagccaa taaaatggca
      961 aaaagcctta cggcaccttt attctgcatt gattttttgg tatttttaat ttttaggcgc
     1021 gcaaatatag aatttgaatt cacattgcac aattttattg aaaccgcatt aaatatgttg
     1081 cataagccaa caaaatcaac tatttacgat aatatttatt attttaaaat gataaaaaaa
     1141 gttgacgttt atcgaatcaa ttgtttgcaa tagttttaaa taacctaaac aaaaagttgt
     1201 aaaaatttat tgcaatttaa attagcataa gaactacaaa aggtttgtta gtatgtttga
     1261 agccacaaaa aacggcctga tgaaaaaaat atttctcttc ctatctgtag cgcttacata
     1321 tcaatttggc aatgcgcaaa caaaatttgt acgcgattta aacattccat taactcaata
     1381 tggcttgcca gcaccattag cgtatagtgg tgggattaat ggtggtatgt tttcagaaat
     1441 tgatttgaat aatgatggta ttaaagattt gtttttgttt gacaaagatc aggtatataa
     1501 gaaagcaaga ataagtactt ttttaaatac aggtacgccc aatactgtca gctaccaata
     1561 tgcacctgag tatattgcta atttcccaac aaaccttgcc gactgggcta tattggtaga
     1621 ttataatcat gatgggaaag aagatatttt tacttatagt aatcagatac ccgctgcagg
     1681 tattaccgtt tataaaaaca ttagcacacc aacacagtta caattcgctt tagaatattc
     1741 attattgtat gcgaatatcc cgtcaggcca aggcaacatc tacgtaagct ctaacgacaa
     1801 acctgctatt gtagattttg atggtgatgg cgacttagac attattgcca tttacattgg
     1861 tggtggcagg ttagagtggt cgaaaaacat aggaatggaa actttaggta aaaatgatac
     1921 attaattttc gactggcaac catcatgttg gggtcatttt ggttttagcg caaataataa
     1981 cagtgccata ttaaatgtgg catgcaaacc catagcattt gtaaacaatg gcgatttgcc
     2041 ggctactttg ctcacaccgg aacctacacc cgaccctacc cgacataatg gtgcctgttt
     2101 tgtaattgag gattttaata aagatggatt ggttgactat ttaggtggcg atgccattgg
     2161 aaataatatt ttgtacctgc aaaatggagg tactttatcc gacccattaa tgattagcca
     2221 ggactccttg tttccggttt acgacaatag tgcatttttc tttgagtttt taaaccccac
     2281 acatattgat gtgaataatg acggaaaaaa ggacttatta gtatcgccct gtaaaaatgg
     2341 cgatgccgaa aactttcata atgtatggta ctacaaaaac gtaggaacag ttgctgatag
     2401 tttttct
//
LOCUS       DAOMAA010000256         2403 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_39889_length_2403_cov_2.994463, whole genome shotgun sequence.
ACCESSION   DAOMAA010000256 DAOMAA010000000
VERSION     DAOMAA010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39889_length_2403_cov_2.994463"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(184..873)
                     /locus_tag="PLO59_04295"
     CDS             complement(184..873)
                     /locus_tag="PLO59_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HQV00355.1"
                     /translation="MQQLDSRVDEIRIIRGELPMEVQDLQDEVEGINARIENIHEEIN
                     NQQEAIMKKKQAVKDANAQIKKYESQQMNVKNNREYDSLSKEIEYQQLDIQLSEKRVK
                     EYTYDLNIKNQELEDLKTSHAERSQVLEQKKNELDIIIGETQKEEVRMMDESEKASKL
                     IDANLLTAYKRVRKSMINGLAVVKIHRDACGGCFNKIPPQMQLEIGQRKKVTFCEHCG
                     RILVDTNINDW"
     gene            complement(1308..2387)
                     /locus_tag="PLO59_04300"
     CDS             complement(1308..2387)
                     /locus_tag="PLO59_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HQV00356.1"
                     /translation="MVAFLEHHAPKAWQESYDNSGLLTGFLNQEVKGVLISLDCIETV
                     IDEALALGCNMVVAHHPILFSGLKKLNGNNYIERTIIKAIKNDVALYAIHTNLDNVWH
                     GVNFKLAEVLGLTPVQVLLPLQKTLKKLEVMVPESHAAAVRDALFDVGCGSIGLYSNC
                     SFNLKGHGTFKARAGAKPFVGQIEADHIEPETKIELVFNANLQQQTETALKNAHPYQT
                     PAYQIIDINNTQQLGSGLLAQLPKPIEEMTFLQLLKERLNVPCIRHTEPLNRPISTIA
                     LCGGAGSFLLPRAITLKAQAFVTADFKYHQFFDADGRILITDVGHYESEQFTKQLLYD
                     LLKGKFNTFALHLSKVNTNPVKYFT"
BASE COUNT          616 a          452 c          443 g          892 t
ORIGIN      
        1 atttatatga gcgttaaaat atattaaggc ataggccggt ttgctaaatg gtataaaaaa
       61 caaaagcaac aacaaagctt tgtaaagttc ccctattttt gaaagcataa agttataata
      121 caaaaaaagg atttctgagt taaagaaatc cctttgtaat tgaacgttta aactgtttaa
      181 atattaccag tcgttaatgt ttgtatctac taaaatacgg ccacaatgtt cgcaaaaagt
      241 aacctttttg cgttgcccta tttctaattg catttggggt ggaattttgt tgaaacaacc
      301 gccacaagca tcgcggtgaa ttttaactac agccaaacca ttaatcatgc ttttacgcac
      361 gcgtttataa gcagtaagca aattggcatc aattaatttt gaggcttttt cagattcatc
      421 catcatacgc acttcctctt tttgtgtttc gccaataatt atatccaact catttttctt
      481 ttgctcaagt acttgcgagc gttcggcatg tgatgttttt aaatcctcta actcctgatt
      541 ttttatattt aagtcgtagg tatattcctt aacacgtttt tctgaaagtt gaatatccaa
      601 ttgctgatat tcaatttctt tcgaaagcga atcgtactct ctgttatttt taacattcat
      661 ttgctgcgat tcgtactttt taatttgcgc attggcatct ttaacagcct gcttcttttt
      721 cattatggct tcctgctggt tgttaatttc ctcatgtatg ttttcaatcc gtgcattaat
      781 gccttcaacc tcatcttgca aatcttgtac ttccatagga agttcgccac gtattattcg
      841 tatttcatca acacgtgagt cgagttgttg catgtagtat aaggcttgca gtttttcttt
      901 taccccaaag ttttgtttat cgctacgggt aaatggctta agttcttctt tcggaattgc
      961 ttttagtata gccgttgggt cgttttttaa tttgatgttt tttgatactt taacctcagg
     1021 tgctttttta actgctttgg tttctttaac ggtttcaaca ccttttttgg cggctttttt
     1081 agttactgct gttgacattt ttttatttga ggattcgtgt ttggatgctg attgcgctgt
     1141 ttttacttta cttttttcta ttactttact ttttgaggca ggctctgttt cttttatttt
     1201 ttttgttgca atggtttttg aggctggctt atctttagca gatacactct tttttaatgc
     1261 ggttttgggc gcatcctttt ttgttgccgg tgccttttta gttgccatta cgtaaagtat
     1321 tttacaggat tggtattaac ttttgacaaa tggagcgcga atgtattaaa ttttcccttt
     1381 aataaatcgt aaagtaattg tttggtgaat tgttcgcttt cgtaatggcc cacatcggtt
     1441 attaaaattc tgccatcggc atcaaaaaac tggtggtatt taaaatcggc tgttacaaat
     1501 gcctgggctt ttaaggttat ggcccggggc aataaaaaac tgccggcacc accacataag
     1561 gctattgtac ttatgggccg gtttaatggc tcagtatgcc tgatacaagg tacatttagc
     1621 cgctctttta atagttgtaa aaaggtcatt tcttcgatgg gtttgggcaa ttgcgccaat
     1681 aagccactac ccaactgttg cgtattattt atatcaataa tctgataagc aggggtttgg
     1741 tacgggtgag catttttaag cgctgtttcg gtttgctgtt gcaggtttgc attaaaaaca
     1801 agttcaattt tagtttcggg ttcaatatgg tcggcttcaa tttgtcccac aaaaggtttg
     1861 gctcctgcac gggctttaaa tgtaccatga ccctttagat taaaactaca attgctgtac
     1921 aagcctatac tgccacatcc tacatcaaat aaggcatcgc gtacggctgc tgcatggctt
     1981 tcgggcacca taacctccag tttttttagc gttttttgta agggtaacaa cacctgcacc
     2041 ggggttaaac ccaatacctc ggctaattta aaatttacac catgccaaac attatccaaa
     2101 ttggtgtgta ttgcatacag tgctacatca ttttttattg cttttataat ggtacgctct
     2161 atataattat taccgttaag ctttttaagg ccgctaaaca atatggggtg atgcgctaca
     2221 accatattgc atcctaaggc aagggcttcg tcaataacag tttcaataca atcaagcgat
     2281 ataagtacgc cctttacttc ttgatttaaa aagccggtta aaaggccgga gttatcatag
     2341 ctctcttgcc aggctttagg tgcatggtgt tctaaaaatg caacaatgtt tcctacggtt
     2401 tcc
//
LOCUS       DAOMAA010000257         2396 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_40109_length_2396_cov_3.395130, whole genome shotgun sequence.
ACCESSION   DAOMAA010000257 DAOMAA010000000
VERSION     DAOMAA010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40109_length_2396_cov_3.395130"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="PLO59_04305"
     CDS             <1..504
                     /locus_tag="PLO59_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HQV00357.1"
                     /translation="KVAGLPITKFVAACNANQVFMQYLENGTYKPQQAIATISNAMDV
                     GNPSNFVRIMHLFNNHYNLVKEAVVSYSISDAETKATIKDVYQKYGYILDPHGAVAYA
                     ALQRYSPTGIILETAHPIKFNTTVESAINTQVPMPASVTNLLRLKKDALLLPKTYETF
                     KHYLMQR"
     gene            complement(571..1146)
                     /locus_tag="PLO59_04310"
     CDS             complement(571..1146)
                     /locus_tag="PLO59_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00358.1"
                     /translation="MSQKVKLQVGFIALLIFLAAFSRVIPHMLNFSPLAAIGLFGAAH
                     FSKKWLAFVIPLAATWLSDLFINNVIYGQYYDGFVWFYDGFHWQYGSYAFITLLALPL
                     FSKITAGRVATGSLMATVVFFLVSNFGCWVGSVMYAPDASGLTACYIAGLPFLKGTFM
                     GDVFYSTVLFGGYYLLQNRYSAFKLSHVTYN"
     gene            complement(1214..1894)
                     /locus_tag="PLO59_04315"
     CDS             complement(1214..1894)
                     /locus_tag="PLO59_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00359.1"
                     /translation="METTFAIDQVMVRYDLDKAHRILYSYDALKQLDVNHAFIMQLLD
                     LFENEASFKAENFDAFSLAVIVDYIQKTHHYYLSKKLLEIEQNITLLLKDYESQHPLL
                     AVLHNFYTRYHKHLADHIQEEEAVLLPYILALESKNLTITNSFKSMHVGFSLQHFIES
                     HHDVEDDLNVARQAILKYNPPATNSTPYRILLSQLQALEKDLAIHALIEDHVLIPRAL
                     QLERDLYN"
     gene            complement(2025..>2396)
                     /locus_tag="PLO59_04320"
     CDS             complement(2025..>2396)
                     /locus_tag="PLO59_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00360.1"
                     /translation="WGKMDAAQMLAHLNVAYDIAFERQVVKHNAFQKLLMKLVVKNAV
                     TNTKPYAKNLRTAPQFVISSNKHFETECDALIQNIKLVEQKGINYFDGKVNQSFGPLT
                     AKQWSNMFYKHIDHHFNQFGI"
BASE COUNT          788 a          443 c          420 g          745 t
ORIGIN      
        1 aaagttgccg gcttgcccat tacaaaattt gtagcggcct gtaatgccaa ccaggtattt
       61 atgcaatacc ttgaaaatgg cacctataag cctcaacaag ctattgctac catttcaaat
      121 gccatggatg tgggcaaccc cagtaacttt gtgcgtataa tgcacctgtt taacaaccat
      181 tacaacctgg ttaaagaagc agttgtaagt tatagcataa gcgatgctga aactaaggca
      241 actattaaag atgtgtacca aaaatatggc tatattttag atccgcatgg tgcagttgct
      301 tatgcagctt tgcaacgtta cagcccaacc ggtataattt tagaaactgc tcatcctatt
      361 aaatttaata ctactgtcga atctgcaata aatacacagg ttccaatgcc ggcatcagtt
      421 accaatttat tgaggcttaa aaaagatgca ctgttattgc ctaaaacgta tgaaaccttt
      481 aagcactatt taatgcaacg ataaatttaa agtgcttagc agctaaatcg aaaatcgttt
      541 taaaaccatt gtttaattta ttgcatcaaa tcagttatag gtaacatgcg ataacttaaa
      601 cgcactataa cggttttgca gtaaataata tccgccaaac aaaacggtgc tataaaaaac
      661 atcgcccata aaagtacctt ttaaaaaagg caaaccggct atataacatg ctgttagccc
      721 acttgcatca ggtgcataca ttacgctacc aacccaacat ccaaaattag acaccagaaa
      781 aaacacaacg gtagccatta acgaaccggt agccacacga cctgctgtta tcttactaaa
      841 caatggtaat gctaaaagtg ttataaaagc atagctacca tattgccaat gaaaaccatc
      901 gtaaaaccaa acaaagccat cgtaatactg accataaatt acattattaa taaacaaatc
      961 gcttagccag gtggctgcca atggtattac aaaagccaac catttttttg agaaatgagc
     1021 agcaccaaat aaacctatag ctgccaatgg cgaaaaattt agcatgtgag ggataacgcg
     1081 gctaaatgca gccaaaaata tgagcaacgc tataaagcca acctgtaatt ttactttttg
     1141 tgacatggta ttaatttcaa ttgatgaatg taaatgtatt tgttttagtg gtgcttttta
     1201 agcagtagtt caattagttg tacaaatcac gttctaactg taaagcacgg ggaattaata
     1261 cgtggtcttc tattaaagca tgtatagcca agtctttttc taatgcctgc aattgcgata
     1321 ataaaatgcg gtatggtgtg ctgttggttg ccggaggatt atattttaaa atggcctgcc
     1381 gggcaacatt taaatcgtcc tccacatcgt ggtgactttc gataaaatgt tgtaatgaaa
     1441 atccaacgtg catactttta aatgaattgg taatggtcag atttttactt tctaaagcta
     1501 aaatataggg taatagtact gcctcttcct cctgaatatg gtcggccaga tgtttgtgat
     1561 agcgtgtgta gaaattatgc aatactgcca gcaacggatg ttgcgattcg tagtctttca
     1621 aaagcagtgt gatattctgc tcaatttcaa gtaatttttt acttaaataa taatgatgtg
     1681 ttttttgaat ataatcaaca ataaccgcca atgaaaatgc atcaaaattt tcggctttaa
     1741 acgaggcttc gttttcaaac aagtcaagca attgcattat aaatgcatgg tttacatcta
     1801 attgttttaa tgcatcgtat gagtataaaa tacgatgtgc tttatccaaa tcgtacctaa
     1861 ccattacctg gtcaatagca aaagttgttt ccatgtgcaa taatgtttgg ttcaaagtaa
     1921 atgctttgaa taaaaagcgc aaatcattaa ggctttaatt tttataactg tttgtcaaca
     1981 aaaacggata tcccttttat gaatatccgt ttttaatggt gtttttaaat accaaactgg
     2041 ttaaaatgat ggtcaatgtg tttgtaaaac atgttgctcc attgtttggc tgtaagcggg
     2101 ccaaacgatt ggttaacttt cccatcaaaa tagtttatgc ctttttgttc aaccagttta
     2161 atgttttgga ttagcgcatc gcattcggtt tcaaaatgct tattacttga aattacaaac
     2221 tgcggtgctg tacgcaggtt tttagcataa ggctttgtat tggttacggc attttttaca
     2281 accagcttca tcaacagttt ttgaaatgca ttgtgcttaa ctacttgcct ttcgaatgca
     2341 atatcataag caacatttaa gtgtgcaagc atttgcgcgg catccatttt gcccca
//
LOCUS       DAOMAA010000258         2391 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_40250_length_2391_cov_3.293664, whole genome shotgun sequence.
ACCESSION   DAOMAA010000258 DAOMAA010000000
VERSION     DAOMAA010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40250_length_2391_cov_3.293664"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="PLO59_04325"
     CDS             complement(<1..439)
                     /locus_tag="PLO59_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008507170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HQV00361.1"
                     /translation="MSSIDWFVLIFTITVIVIYGIWKSRGTKTIDTFLLADRKFNGWF
                     VGFSVMATQASAITFLSAPGQGFTDGLRFVQFYFGLPLAMIAICIWFIPVYKSLHVFT
                     AYEYLEQRFNRRMRLLTAALFLFQRALSTGVTIYAPALVLSTIL"
     gene            complement(536..>2391)
                     /locus_tag="PLO59_04330"
     CDS             complement(536..>2391)
                     /locus_tag="PLO59_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="LmbE family protein"
                     /protein_id="HQV00362.1"
                     /translation="NFGGNNTTADNQIKVDVGLYNVLMGAGYGEIAANSRSNHKSQGF
                     GSARGRGSNLEYFKQLKGDNVNTDILEGIDITWTRFNANNIRSAIEKCIAQFDALAPD
                     KSITTLLHIYQQLQLLDTSDAGLKYWQAQKTKEVTQLIFACAGLWVEAAATEFTGIAG
                     NAFTCNIQAINRSNAQVYLKSITITNDTLVQLPLQNNVLFTVKHTETLPSTIAFSNPY
                     WLNETGTYGLFNVQDQQLIGQPLNKSNATVTFNLTINQLPFTITQPVVYKYTDPVKGE
                     VYRPFEILPPATVNMADKVCISANGKPVKVSFVIKANKHNVSGKLLLRVPKGWQADTS
                     LFSFNLTNKNDELIFEAILKPGTQTTNGILSADLEIEGKKYNQSIQRINYDHIPNQFI
                     LTPAETKLVKLNCQTGGKKVAYINGAGDEVADCLKQLGFEITTLEVSQISKTNLHAFD
                     AIITGVRAYNVHDDLPLHYQQLMDYIKQGGNLIVQYNTNSRVGPVKSKIGPYPFTISR
                     DRVTNENAAVNFITPHIALQQPNKIATSDFENWVQERGIYFATEVDSNYVMPLSMADP
                     NEKASNGSLLIAPYGKGNFVYTGLAFFRQLPAGNTGAYRLIANLLALPKHE"
BASE COUNT          694 a          464 c          457 g          776 t
ORIGIN      
        1 ctaaaatggt tgacaacacc aatgccggtg cataaatggt tacgcctgtt gataatgctc
       61 gttgaaataa aaacagtgca gcagtaagca agcgcatgcg cctgttaaaa cgttgttcta
      121 aatactcgta ggctgtaaaa acatgcaaac ttttataaac gggtataaac caaatgcaaa
      181 tagcaatcat ggctaaaggc aaaccaaagt aaaactgcac aaatcgtaaa ccatcggtaa
      241 agccttgccc cggtgccgat aaaaatgtga tggcactggc ttgagtagcc ataactgaaa
      301 aaccaacaaa ccaaccatta aattttctgt cggctaataa aaaggtgtct atggttttag
      361 tacctctact tttccaaatt ccataaatca caattaccgt tatggtaaat atcaatacaa
      421 accaatcaat actactcatg caaaccagat tgtaaataca tacattaata aaattataag
      481 tagcaggaaa aataccagcg ctacatataa taccggccag tttatttttg gttcgttatt
      541 catgtttggg taatgcaagc aggttagcta ttaagcggta tgcgcctgta ttaccggccg
      601 gtaattgtct gaaaaatgcc aggcctgtat aaacaaaatt acccttacca tagggtgcaa
      661 taagtaaact gccattactt gctttttcat tcgggtcggc catgctaagt ggcattacgt
      721 aattactatc aacttcggtt gcaaagtaaa ttccgcgttc ctgtacccaa ttttcgaaat
      781 cggaggttgc aattttatta ggctgctgta acgcgatatg cggtgttata aaatttactg
      841 cagcattttc attggttact ctatcgcgtg atatggtaaa cggataagga cctatttttg
      901 attttaccgg tcctactctg ctattggtat tgtattgcac tattaagtta ccgccttgct
      961 ttatataatc cattaactgt tggtagtgca gcggcaaatc gtcatgtaca ttataggcac
     1021 gtacaccggt tataatcgca tcaaaagcat gcaggtttgt tttgctaatt tgagatactt
     1081 ccaatgtagt aatttcaaat cctaattgtt tcaaacagtc ggctacttcg tcacctgctc
     1141 catttatata tgcaactttt ttacctccgg tttggcagtt aagttttacc agtttggttt
     1201 cggcaggtgt aagaataaat tggttaggta tatggtcgta gttaattctt tgtatgcttt
     1261 gattgtattt ttttccttca atttctaagt cggccgatag tattccgttc gtggtttgtg
     1321 taccgggttt aagtatggct tcaaatataa gctcgtcatt tttattggtg agattaaatg
     1381 aaaacaaact tgtatcggcc tgccagcctt ttggtacacg taacaataac ttgccactaa
     1441 cattatgctt atttgccttt ataacaaatg aaactttaac cggcttacca tttgccgaga
     1501 tgcaaacttt atcagccata tttacagtag caggcggcag tatttcaaat ggcctataca
     1561 cttcaccctt taccggatcg gtgtatttat aaaccacggg ttgcgtaatt gtaaatggca
     1621 gctgattaat agtgagatta aatgtaacag tggcgttact tttatttaac ggttgtccaa
     1681 taagctgttg gtcttgcaca ttaaacaagc cataagttcc tgtttcgtta agccaatatg
     1741 ggttactaaa tgcaattgtt gacggtaatg tttctgtatg ttttacagta aatagcacat
     1801 tgttttgcaa gggtagttgc accaacgtat cgttagttat ggttattgat tttaaataca
     1861 cctgtgcatt gctgcggtta atagcctgta tattgcaagt aaaggcattg ccggcaatac
     1921 ccgtaaattc agtagccgca gcttcaaccc acaaacctgc acaggcaaat atcagttggg
     1981 ttacttcttt ggttttttgt gcctgccagt attttaaacc ggcatcggag gtatctaaca
     2041 actgtaactg ttggtatata tgcaaaagtg tggtaatact tttatcggga gcaagtgcat
     2101 caaattgtgc aatacatttt tcgatggctg atctaatatt atttgcatta aagcgcgtcc
     2161 aggtaatatc aatgccttct aaaatatcgg tattaacgtt atcgcccttt agctgtttaa
     2221 agtattctaa gtttgaaccc ctgcccctgg ccgatccaaa accctggctt ttatggttgc
     2281 tgcggctgtt ggctgcaatt tcgccataac cggcacccat taaaacgttg tataagccta
     2341 catctacttt tatctgatta tcggctgtag tattgttgcc accaaaatta a
//
LOCUS       DAOMAA010000259         2389 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_40310_length_2389_cov_2.892888, whole genome shotgun sequence.
ACCESSION   DAOMAA010000259 DAOMAA010000000
VERSION     DAOMAA010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40310_length_2389_cov_2.892888"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="PLO59_04335"
     CDS             complement(<1..687)
                     /locus_tag="PLO59_04335"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQV00363.1"
                     /translation="MELNYYQLGSGKPLLILHGLFGQSDNWLTLAKRWATTHTVYVID
                     LRNHGKSPHDIVFTHAAMAQDVVAFIERNSLQDVTLMGHSLGGKVAMYVALNYQAIQK
                     LIVVDIAPRYYKPHHQSIINGLYAVPLHQLTSRNMAEPFLEPFIDDAGTRQFLLKNLY
                     WKTDTQLAWRFNLDAIASNIDQVGAAVTGKPFQRPALFINGGKSNYITSNDSNLINIL
                     VKIIENEKGLI"
     gene            825..1280
                     /locus_tag="PLO59_04340"
     CDS             825..1280
                     /locus_tag="PLO59_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013928794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQV00364.1"
                     /translation="MHTNIEITPGTTAHVEAAFALVKELAEFENGLDQVSNTASQMLL
                     DGFGPDPCFSLLVALENQVVVGIAIYFTKYSTWKGKGIYLEDIVVTKAHRGKGIGKML
                     FDAVWLEAQKQNAHQLHWQVLDWNKEAISFYKKYPATFDQEWINCKICW"
     gene            complement(1332..2135)
                     /locus_tag="PLO59_04345"
     CDS             complement(1332..2135)
                     /locus_tag="PLO59_04345"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HQV00365.1"
                     /translation="MIKKCARMLALLCILSLSLSMGLAQTLPDRPNPPHLVNDFAGIF
                     STEESQALEIKLVTYADSTSTQIAIVTLQSLDGYPIDDYAFKLGNKWGIGEKGKNNGL
                     LILVSVADRKAFIATGYGMEGALNDGKLGTILRNEILPYFKGGNYYQGIDNGINSIML
                     AAAGEYTGSGKSKKRIDTGSLLTVLFIVVIIISIGLFTTYRSVKRYSIMNNVPFWVAW
                     NIINAASRHNNRGGGFWGGGGGGGGFGGGGGFGGFGGGSFGGGGAGGSW"
     gene            complement(2149..>2389)
                     /locus_tag="PLO59_04350"
     CDS             complement(2149..>2389)
                     /locus_tag="PLO59_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013664040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HQV00366.1"
                     /translation="YLSVDDHKYAIIGDTGIHLKVGIDFWNEAKEAMLMHFKQGNLTE
                     GIIAGISKAGEALSKHFPRAHDDTNELQNDVVFGD"
BASE COUNT          730 a          510 c          433 g          716 t
ORIGIN      
        1 tatcaatcct ttctcatttt ctatgatttt tacaagtata ttaattaaat tagaatcgtt
       61 ggaggttata taattcgatt tgcccccatt tataaacagt gccggccttt gaaacggctt
      121 acctgttaca gcagcaccaa cctgatcaat atttgaagct attgcatcta aattaaagcg
      181 ccatgctaat tgtgtgtcag ttttccagta taaatttttg agcagaaact gccgcgttcc
      241 tgcatcatca ataaatggtt ctaaaaatgg ttcggccata ttgcgtgatg taagttggtg
      301 taatggcaca gcatataatc catttataat actttgatga tgtggcttat aatatcgggg
      361 ggcaatatca actacaataa gtttttgtat agcctgataa tttaacgcca catacatggc
      421 aactttaccg cccaatgaat ggcccattaa agttacatcc tgcaaactat tgcgctcgat
      481 aaacgcaact acatcctgtg ccatggccgc atgtgtaaac acaatatcgt gaggcgattt
      541 gccatgatta cgcaagtcaa taacatatac cgtatgcgta gttgcccacc gcttggcaag
      601 tgtaagccaa ttgtcgcttt ggccaaacaa gccgtgcaaa attaatagcg gcttgccgct
      661 gcctaactgg taataattta attccatgtg ctattacttt atccaacaca aatctattga
      721 agcgaattta aaaaatggat gtatgcatat cagaattagc aaatggattt attagatata
      781 attgtattta aatttttatc agcattaata cttaaaaaat atacatgcat acaaacatcg
      841 aaataacgcc aggcacaaca gcacatgtgg aagcagcttt tgccctggtt aaggaattag
      901 ccgaatttga aaacggatta gatcaggtaa gtaacacggc atcgcaaatg ttgcttgatg
      961 gatttggccc tgacccatgt tttagtttac tggtagcgtt agaaaaccag gttgtggtag
     1021 gtattgccat ttactttacc aaatactcga cctggaaagg caaaggcatt taccttgaag
     1081 atattgttgt tacaaaggca catcggggta aaggaattgg caaaatgtta tttgatgccg
     1141 tttggctaga ggcccaaaaa caaaatgcac atcagttgca ctggcaggtg ctcgattgga
     1201 ataaggaagc tatttcgttt tataaaaagt atccggctac ttttgatcag gagtggatta
     1261 attgtaaaat ctgctggtag tttattgtta cattttgtag ttcgatcaat gcaagtgttc
     1321 tgaccacaaa attaccagga cccacctgca ccacctccac caaaacttcc gccaccaaaa
     1381 cctccaaaac caccgccacc accaaagccg ccaccgcctc caccaccacc ccaaaaacca
     1441 ccaccgcggt tattatggcg cgatgcagcg tttataatat tccacgctac ccaaaatggt
     1501 acattattca ttattgaata acgtttaacc gaacgataag tggtaaacag accgatagaa
     1561 ataattataa caacaataaa aagtacggtt agtaagctgc ctgtatcaat tcgttttttt
     1621 gatttgccac tacctgtata ttcacctgct gctgccagca taatgctatt aataccatta
     1681 tcaatacctt gataataatt accaccttta aaataaggca gtatttcatt gcgaagtatg
     1741 gtgcccagtt ttccatcgtt taaagcacct tccataccat aaccggttgc aataaaagct
     1801 ttacggtcgg ctacggatac taaaataagt aagccattat ttttaccttt ttctccaatg
     1861 ccccatttat tacccaactt aaaagcataa tcgtcaatgg ggtatccgtc taaactttgc
     1921 agggtaacta ttgcaatctg tgtagaagtg ctatcggcat aggttaccaa ttttatctcg
     1981 agcgcttgcg actcttctgt gctgaaaatt cctgcaaaat cgttaaccaa gtgtggcggg
     2041 tttggcctat cgggcaaagt ttgcgccaag cccatgctta aacttaagct tagtatgcat
     2101 agcagggcta acatgcgtgc acactttttt atcattaccg ttttcatatc aatcgccaaa
     2161 cactacatca ttttgtaact cattggtatc gtcatgtgcg cgcggaaaat gtttacttaa
     2221 cgcttcgcct gctttgctta tgcctgcaat aataccttcg gttaaattac cttgcttaaa
     2281 gtgcataagc atagcttctt tggcctcatt ccaaaaatcg ataccaactt taaggtgtat
     2341 gcctgtatcg ccaataatgg cgtatttatg atcatcaaca gaaaggtaa
//
LOCUS       DAOMAA010000260         2388 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_40338_length_2388_cov_3.256751, whole genome shotgun sequence.
ACCESSION   DAOMAA010000260 DAOMAA010000000
VERSION     DAOMAA010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40338_length_2388_cov_3.256751"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..980)
                     /locus_tag="PLO59_04355"
     CDS             complement(87..980)
                     /locus_tag="PLO59_04355"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV00367.1"
                     /translation="MLKTDSLGNTVWTTPCTGNFNNLVIGYNENILAVGGINNSYLAM
                     LLDKNGSIIWTKNYGTLSTIQGIDMRILNAVQLADSNFATINSTCQFTPIVGWNYMTI
                     DRNNGDSLFSKSFPGSCFDYNFASITNSNDSNLFICLESEIIKMSPSGNILWSNYNAH
                     NFYFSIVRCNDNSAAAGGNYWWYDQPGEPYFEESVVVKLDSLGKLNPTSGMYSHENTG
                     NYLYPNPVKTAFSLNHLLPSEKIVTVTIYDAFGKEKMKHTNVSGIDNTYQFDVSHFEQ
                     GIYFLEILAPTEVFRYKFIKE"
     gene            complement(1062..1406)
                     /locus_tag="PLO59_04360"
     CDS             complement(1062..1406)
                     /locus_tag="PLO59_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00368.1"
                     /translation="MKIKITLLFILFLIQSNAQQITWNKKYFQPIKHFNAAISINQLP
                     DSGFIILANKYNVGSYGKVIRTNKYGDSIWVKIIEVTQKLITTYDNGFMLSGYDGNGS
                     FLKKLNSNGDTI"
     gene            1753..>2388
                     /locus_tag="PLO59_04365"
     CDS             1753..>2388
                     /locus_tag="PLO59_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HQV00369.1"
                     /translation="MLTEQQKKQQGHTISLPVLLKAYSTLCTAKALTDLYEENFSFVS
                     KYVHATSRGHEAIQIAVGMQLLPQDYVSPYYRDDSMLLAIGLQPYELMLQLMAKRDDP
                     FSGGRTYYCHPSLRRDDMPKIPHQSSATGMQAIPCTGVAMGMWYQEQMQLHDATKPLP
                     VAVCSLGDASVTEGEVAEAFQMSVLKSLPILYVVQDNGWDISANANETRNMN"
BASE COUNT          774 a          438 c          409 g          767 t
ORIGIN      
        1 ttacaacccg actgctatca gatatcaaat aaacattggt ttcgttacat gattatttcg
       61 aaacagtacg atttatttgg tgcgccctat tcctttataa acttgtatct aaaaacctcg
      121 gttggggcaa gtatttctaa aaaataaatt ccttgttcaa aatggcttac atcaaattgg
      181 taggtattgt caatacctga aacattggtg tgtttcattt tttcttttcc aaaagcatca
      241 tatattgtaa ctgtaacaat tttttcagat ggaagcaaat gatttaacga gaaagcagtt
      301 ttaaccggat tggggtataa ataattgccg gtgttctcat gcgaatacat gcccgatgtg
      361 gggtttagtt tacctaaact gtccaattta acaacaacag attcttcgaa atagggctca
      421 cccggttgat cataccacca atagttaccg cccgcagcgg cactattatc attacaacgg
      481 acaattgaaa aataaaaatt gtgggcatta taattagacc aaagtatatt gcctgatgga
      541 ctcattttta taatttctga ttccagacat ataaacaaat tcgaatcgtt gctgttggta
      601 atactggcaa agttgtaatc aaaacagcta cctggaaacg atttgctaaa taaactatcg
      661 ccattatttc tatcaattgt catataattc catcctacaa tgggcgtaaa ttgacaagta
      721 gaatttatag tagcaaagtt tgaatcggcc aattgcactg catttagaat tctcatgtca
      781 atgccctgaa tagtactaag cgtcccgtag tttttagtcc aaattattga gccattttta
      841 tcaagtaaca tggccaaata actattgtta atacctccaa cagctaaaat attttcatta
      901 taaccaataa ccagattatt aaaatttcca gtacagggtg tagtccaaac agtattgcct
      961 aacgagtcag tttttagcac aaaattagac ccacataata tatagccact gtcattgctt
     1021 tgtgtaacat aacatgcgct gatataagga taatactttt tctaaatagt atcgccattg
     1081 ctatttaact tttttaaaaa gctaccgttg ccgtcatatc ccgataacat gaagccgtta
     1141 tcataagttg taataagctt ttgtgtaacc tcaattattt ttacccatat actatcgcca
     1201 tatttattgg tgcgtattac tttaccgtac gaacccacat tatatttatt ggcaagtatg
     1261 ataaatccac tatcaggtaa ctgattaata ctaatggcag cgttaaaatg ctttataggt
     1321 tgaaaatatt ttttattcca tgttatttgt tgggcattgc tttggattaa aaataatatg
     1381 aataataaag taattttgat tttcatgaat atcgagatta gtaacttatt aaatcgattc
     1441 atacgaatat agaaaagttt aatttaaagg taatttgtta tgcatgttat aaatacaata
     1501 cactgtcata tacaaaatat ttggatggtg tacttgtctt ttgtaaatat taccgctaac
     1561 cgaaggccga agtatttccg ctctcacaaa gttagtttta aagcagacaa aaatcacatt
     1621 cagctttttg ataaataaat gcacaaactt gcaataacca catctgcttt ttacacatgt
     1681 taaagccgca tgttgtagta attgtaacat ttatagcttt tatttattga tggtaagcca
     1741 taggtttgcc ccatgttaac cgaacaacaa aaaaagcaac aaggtcatac catatcctta
     1801 ccggtattac taaaggcgta tagtacctta tgtacagcca aagcgctaac cgatttgtat
     1861 gaggaaaatt tttcttttgt ttcaaaatac gtacacgcca catcgcgtgg gcacgaagcc
     1921 attcaaattg ctgtgggtat gcaattgttg ccgcaggatt atgtaagtcc ttattatcgt
     1981 gatgatagta tgttactggc tattggcttg caaccgtatg aactgatgtt gcaattaatg
     2041 gccaaacgtg acgacccttt tagtggtggc cgcacctatt actgccatcc cagtttacgt
     2101 agagatgata tgccaaaaat accccatcaa tcatcggcca cagggatgca ggcaatacca
     2161 tgcaccggag tagctatggg tatgtggtac caggagcaaa tgcaattaca cgatgccaca
     2221 aaaccattac cggttgccgt atgttcctta ggcgatgcat cagttaccga aggcgaagta
     2281 gccgaagcat ttcaaatgtc tgtacttaaa tcgttgccta tattatatgt ggtacaggat
     2341 aatggttggg atatatcagc caatgccaat gaaacacgta acatgaat
//
LOCUS       DAOMAA010000261         2381 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_40586_length_2381_cov_2.204213, whole genome shotgun sequence.
ACCESSION   DAOMAA010000261 DAOMAA010000000
VERSION     DAOMAA010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40586_length_2381_cov_2.204213"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2273
                     /locus_tag="PLO59_04370"
     CDS             <1..2273
                     /locus_tag="PLO59_04370"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV00370.1"
                     /translation="KREFYYLPNDSDETNNLLKTTVTDSDFVNYQYLCNEMATLLDSG
                     YALCNAMIDASVVISPITCYGQTTQITPTITGAVAPYTYLWSNGSSQQNLTNVTAGTY
                     TLTVTDNVGQNRQFTYHVTQPDSLTATDSIWNCNKAARSMVTGGVKPYTYLWDNGNTT
                     GAQYNLSAGVYTVTITDQNGCTLSNSISIQTIPLLEVTGNATNVLCNGFNNGSVQTTI
                     TGGKWPYNYLWSNGLTTKDAFNLTNATYTLTVTDAVGCKKTTDFIIIETQPETCFFSF
                     TAPECDKFCDGAAQVLSDNTLNGYSFLWSNSATTQSIQNICAGLYTVTISNTNNCTAS
                     ATAEIKGKNLLQFTANVLPANCPKTKTGRANLIVTNGHSPFTYLWNNIPNGVFSNRLI
                     SGFHNISVIDGHGCRYDSTIFIPYLSAPKIIVTVTAVTCNSNQGALLATASNGIPPYS
                     YLWSNGNTTAGISQLSGGTYLVTVTDSLKCKKTLVTQLADTGVVPDKPQPIAGQNNTV
                     CAGTNNVLYQINPVANSIGYHWVIPVNSTLVSGQGTTSIVLNYDSNFVSGTLYAQAFN
                     QCGMGQQRFLTIRSVPAVPSSIIGPVSVCANQNNVLYEILPVPNASSYQWLKPANTQI
                     VSGQGTTNVLLNFQNNGGKVKVMATNVCGNSDAKSLTVNMNCRNGSLQASEIIIMPNP
                     ANDIVQVSFVSEQITAYQIEIFDLMGHVLKGQSKMAEIGDNQCNILLADISAGIYFVT
                     ISIGNETLISKLVIEK"
BASE COUNT          814 a          457 c          429 g          681 t
ORIGIN      
        1 gcaagcgcga attttactac ttacccaatg acagtgatga gacaaataac ctgctaaaaa
       61 ccaccgttac cgatagcgac tttgtaaatt atcaatatct gtgtaatgaa atggcaaccc
      121 ttctcgatag tggttatgct ttatgcaatg caatgataga tgcctctgtt gttatttcgc
      181 ccattacttg ttatggacaa accacacaaa taacacctac cataacaggt gctgttgcgc
      241 cttatactta tttatggagc aatggcagtt cgcaacaaaa cttaaccaat gtaaccgctg
      301 gtacctatac actaaccgtt acagataatg tcggacaaaa ccgacaattt acttaccacg
      361 ttacacaacc ggatagttta actgccactg atagtatttg gaattgcaat aaggcagcac
      421 gcagtatggt tacgggtggc gttaagccct atacgtattt atgggataat ggaaatacaa
      481 caggagcaca atacaaccta agtgccggtg tatatacggt aaccataacc gatcaaaatg
      541 gttgcacatt aagtaattca atttctattc aaacaatacc cttattggaa gtaaccggta
      601 atgccaccaa tgttttatgt aatggcttta ataatggaag cgtacaaaca actattaccg
      661 gaggtaaatg gccatataat tatttatgga gtaatggact taccacaaaa gatgctttta
      721 atttaaccaa cgctacctat acactaacag ttacggatgc tgttggctgt aaaaaaacaa
      781 cggactttat aattattgaa acacaaccgg aaacttgctt ctttagcttt acggcaccgg
      841 aatgcgacaa attttgtgat ggtgctgcac aagtgcttag cgacaacact ttaaatggtt
      901 atagtttttt gtggagtaat agtgcaacca cacaatccat acaaaatatt tgtgccggtt
      961 tatataccgt taccattagc aataccaata actgtacagc aagtgcaaca gcagaaataa
     1021 aaggaaaaaa cttattacaa tttaccgcaa atgtacttcc tgcaaattgt ccaaaaacaa
     1081 aaacaggtcg tgccaatctt attgtaacca atgggcatag cccctttacc tatttatgga
     1141 ataatatacc aaatggtgtt tttagtaacc gtttaatatc aggctttcat aatatttcag
     1201 taatagatgg gcatggctgt aggtacgatt caaccatttt tataccttat ttatcggcac
     1261 ccaaaataat tgtaacggtt acagctgtta cgtgcaacag taaccaaggt gcactgttag
     1321 caactgccag caatggtatt ccaccttatt catatttatg gagcaatgga aacactactg
     1381 ccggcataag tcaattaagc ggtggcacct atttagtaac tgtgaccgat tcattaaaat
     1441 gtaaaaaaac attggtaact caattggccg ataccggtgt tgtacccgat aaaccacaac
     1501 ctattgcagg acaaaataat accgtatgtg ccggaacaaa taatgtgtta tatcaaatta
     1561 atccggtagc taattcaata ggttatcatt gggtaatacc tgtaaacagt actttggttt
     1621 caggtcaagg aaccacatcc attgtattaa actacgattc aaattttgtt tcgggtactt
     1681 tgtatgccca agcatttaac caatgcggca tgggccaaca acgattttta actatccgat
     1741 cagtacctgc agtaccttca agtatcattg ggcctgtaag tgtttgtgct aaccaaaata
     1801 atgtacttta tgaaatttta cctgtaccca atgcaagtag ttatcaatgg ctaaaacctg
     1861 ccaatacaca aatagtaagc ggccagggaa caacaaacgt tctattaaat tttcagaata
     1921 atggtggtaa ggtaaaagta atggcaacca acgtatgcgg taatagcgat gccaaaagtt
     1981 taacggttaa tatgaactgt cgtaatggaa gcttacaagc gtctgaaatt ataattatgc
     2041 caaatcctgc aaacgatata gtacaagtta gttttgtaag tgagcaaata acagcatatc
     2101 aaattgagat atttgattta atgggacatg ttttaaaagg acaaagtaaa atggcagaga
     2161 taggcgataa ccaatgtaac atactgcttg ctgatataag tgcgggaatt tattttgtaa
     2221 caatatcgat tggcaacgaa accctaatat cgaaattagt tattgaaaag taatgggcta
     2281 aacctccgat ccccagtgta taaatctaaa caatctgttc catctgattt tatgtagcca
     2341 gtctgcatca taatctaaac tgagccatac aaaaaccgct t
//
LOCUS       DAOMAA010000262         2377 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_40706_length_2377_cov_2.559432, whole genome shotgun sequence.
ACCESSION   DAOMAA010000262 DAOMAA010000000
VERSION     DAOMAA010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40706_length_2377_cov_2.559432"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..781)
                     /locus_tag="PLO59_04375"
     CDS             complement(98..781)
                     /locus_tag="PLO59_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00371.1"
                     /translation="MYRFRIFHAPTDTETSAKYLEGHVNVLKDYGITNITSNNQEWMT
                     WNCVYGIVAERDDGVLVGGIRVQIADGINMLPVEKAIGKMDPNIHQIVNGYINTGVGE
                     LCALWNAKVVAGKGLSLLLTRAGISIVNQIKCKTLMGICADYTMPMFRRVGFVVDDSL
                     GDNGQFVYPNENYIARVLGILNAKDLGTSQPYDRERMLSLRNSPNQKYTEMGAKGSIE
                     INYQLFLPN"
     gene            complement(783..>2377)
                     /locus_tag="PLO59_04380"
     CDS             complement(783..>2377)
                     /locus_tag="PLO59_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00372.1"
                     /translation="GGKMDLLIEESDGFDIKIISRYAARKHGIPVLMEASDKCMVDVE
                     RFDLEPERPILHGLVEHLNIEKLKQLKTTEDKIPYMMDVLGIDTSSVRLKASMIEIEQ
                     SINTWPQLASAVTMGGGITADVARRVLLGQFNSSGRYYVDVENIIGNQPETKPDNAFK
                     KHPTFKLEDAINFALNYQIKGKDVIGNRIESWLQFAIKAPSAGNNQPWHWVFKNDVLF
                     VIHNATLSYCWSDTDGNLAHLALGAAIENISLTAGMDGYAVEIKHLSHPFCLAALTFS
                     KTKESGNSILFNAIKVRCTNRKHGNGHPINATITTNLDIVAKPATIRWKHDKDSIFAL
                     ADIVASVEKIRFLNPYGHDEFFNKEIRWDTVQAKKTCDGLDIATLELSQIDFTGLKVA
                     SDPAVIEKVSEWKGGDALKKITSNAIKSASAIALLSVDKNLNVLDAGACVQRFWLAAI
                     AQGLHLQPISAPVFYFNKLQGIHHFSENEIAELNKQQIKFEHIFESLPALRDTFLFRV
                     MYAQTPTTIALRKPLENSFTMI"
BASE COUNT          685 a          475 c          423 g          794 t
ORIGIN      
        1 ttgtaatcgc tgctaactat aggaattatt tgggcccggg ttataccggc caacagcagc
       61 aaacttatta gtgtaaattg tttaattaaa aacacagtta attgggcaaa aacagttggt
      121 aatttatttc aatagagccc ttagcgccca tttcagtata tttttgattc gggctgtttc
      181 gcaagcttaa catgcgttcg cggtcatagg gttgtgaggt gcccaaatct ttggcattta
      241 aaattcctaa tacccgcgca atatagtttt cgtttggata aacaaattga ccgttgtcgc
      301 ctaacgaatc atcaaccaca aaaccaacac gcctgaacat aggcatggta taatcggcac
      361 aaatgcccat taatgttttg catttaattt gattaacaat actgatgcct gcacgggtta
      421 gtaataaact taaacctttg ccagcaacta ctttggcatt ccaaagagcg caaagttcgc
      481 ctacacctgt attgatatat ccattaacaa tctgatgtat gttggggtcc atttttccaa
      541 ttgctttttc aacgggtagc atattaatgc catcggcaat ttgtactctt atgccgccca
      601 ccaaaacgcc atcatcgcgt tcggcaacaa tgccgtaaac gcaattccat gtcatccatt
      661 cttggttatt ggaagttata tttgttatgc catagtcttt taaaacgttt acatggcctt
      721 ctaaatattt agcactggtt tcagtatcgg tgggggcatg aaaaattctg aatctgtaca
      781 ttttaaatca tagtaaaact gttttctaaa ggttttcgta gtgcaatagt tgtaggtgtt
      841 tgtgcataca taactctaaa caaaaatgta tctcttaatg caggaagaga ctcaaatata
      901 tgctcaaatt taatttgttg tttgtttagt tcggcaattt cgttttcgga aaagtgatgt
      961 atgccttgca gtttattaaa ataaaatacc ggtgcactaa tgggttgcag gtgtaaacct
     1021 tgtgcgatag ctgccagcca aaagcgttgc acgcaagcac ctgcatcaag tacatttaaa
     1081 tttttatcaa cacttagcag tgctattgct gatgcacttt ttatagcatt agatgtaatt
     1141 ttttttagtg catcaccgcc tttccattcg ctaacttttt cgataacggc cgggtcggaa
     1201 gctactttga ggcccgtaaa atctatttgg ctaagttcta aagtagctat atctaatcca
     1261 tcgcatgttt tttttgcttg tacggtatcc catctaattt ctttattaaa aaattcgtca
     1321 tgcccataag gatttaaaaa cctgattttt tcaaccgaag caacaatatc agccagtgca
     1381 aaaatactat ccttatcatg tttccaacgt atggttgcag gtttggcaac aatatctaaa
     1441 ttggtggtta tggttgcatt aatgggatgc ccgttgccgt gtttgcgatt ggtgcagcgt
     1501 actttaattg cattaaacaa aattgagttg cctgattctt ttgtttttga aaaagttaat
     1561 gctgctaaac aaaacggatg tgataaatgt ttaatttcaa cggcataacc atccatacca
     1621 gctgtaagtg atatattttc aatagcagcc cccaaagcca gatgcgctaa gttgccatcg
     1681 gtatcgctcc agcaataaga cagtgtggca ttgtgtatta caaatagtac atcgttttta
     1741 aatacccaat gccatggttg gttattaccg gctgacggag ctttaattgc aaattgcaac
     1801 caactttcaa ttcggttacc tataacatct ttacctttta tttgataatt aagtgcaaag
     1861 tttattgcat cttccaattt aaaagtaggg tgctttttaa atgcattatc aggtttggtt
     1921 tcgggttggt tcccaataat attttcaaca tcaacataat accggcccga gctgttaaac
     1981 tgtcccaata aaacccttct tgcaacatcg gcagttatgc cgccacccat tgtaactgca
     2041 ctggcaagct gtggccatgt atttatgctt tgttcaattt caatcataga tgctttaagt
     2101 ctgacacttg acgtatcaat acctaaaaca tccatcatat aaggtatttt atcttccgtt
     2161 gtttttagtt gttttagttt ttctatattc aaatgttcaa ccagaccatg taaaatgggg
     2221 cgctcaggtt ctaaatcaaa tcgttcaaca tcaaccatac acttatcgct tgcttccata
     2281 agtacgggta tgccatgttt acgcgcggca tatcggctaa taatttttat atcaaaacca
     2341 tccgactctt caatcaataa gtccatttta ccgccat
//
LOCUS       DAOMAA010000263         2365 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_41120_length_2365_cov_2.811688, whole genome shotgun sequence.
ACCESSION   DAOMAA010000263 DAOMAA010000000
VERSION     DAOMAA010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41120_length_2365_cov_2.811688"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PLO59_04385"
     CDS             <1..227
                     /locus_tag="PLO59_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00373.1"
                     /translation="HQKTSNTVKQLEVKIEKLKEDKALLLLDMAKPDVAANAEKTKSL
                     HSFLITIDKELEKLNVEFDEAYLQLLELEN"
     gene            217..1521
                     /locus_tag="PLO59_04390"
     CDS             217..1521
                     /locus_tag="PLO59_04390"
                     /inference="COORDINATES: protein motif:HMM:NF028426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HQV00374.1"
                     /translation="MKTKIATIPLWLIPFCFLFLQTAAQNNDYANPNVIRYEDYNYNP
                     NIKTVLLEGATGQLTDAAIELNTNQQLLLRFDHLYADVVDYSYTIIHCDANWQPSQIS
                     ESQYLQGFSTDKILRYQFATTTLVPYVHYELLFPNDIIKPIISGNYLLRVFKTDTPEI
                     NVLTKRFIVFSQLAGIQANIHAGTFADTRFTHHEIDFELNTQQLNLLNPYNDLQVVLL
                     QNGGWLQRIYGLKPRFVNGNKLDYNYDGEYCFAGGNEFRTFDTRSIRFLSERIKQITQ
                     ADDKTFIVNLHNDIRTSSQRYAFVNDINGQYLIKHQEGRNSDVDADYVWVNFKLKMPQ
                     EETEGNMYLYGALTNWRTDTTSLMNYNADDMSYEKSLLLKQGYYNYQYAYISSGSYPD
                     VTVTEGSHYETDNSYTLLIYYRNPNTRYDAIVGYKKLNSIKK"
     gene            complement(1567..2019)
                     /gene="dtd"
                     /locus_tag="PLO59_04395"
     CDS             complement(1567..2019)
                     /gene="dtd"
                     /locus_tag="PLO59_04395"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HQV00375.1"
                     /translation="MRALIQRVTHASVHVNNQVAGQIEKGLLVFIGIEDADTTEDVQW
                     LCNKIIKLRIFDDANGVMNEDVIQANGRLLIVSQFTLHALTKKGNRPSYIKAAKPEIA
                     IPIYELFVETCFQLTGHRPATGIFGADMQITLCNNGPVTIFIDSKNKE"
     gene            complement(2027..>2365)
                     /gene="rsgA"
                     /locus_tag="PLO59_04400"
     CDS             complement(2027..>2365)
                     /gene="rsgA"
                     /locus_tag="PLO59_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HQV00376.1"
                     /translation="EPTLTLRTGAISDQHQTGKHTTTFAEMFPLKAGGFIIDSPGIRE
                     LGTVDFLPQEVSHYFVEMRALLPQCKFNNCLHINENKCAVKDALIAGKISPDRYHNYL
                     SIMEGKDLFK"
BASE COUNT          783 a          447 c          423 g          712 t
ORIGIN      
        1 aacatcaaaa aaccagtaac accgttaagc aattggaagt taaaattgaa aaacttaaag
       61 aagataaagc gcttttgctg ttagatatgg ccaaacctga tgtggcagca aatgccgaaa
      121 aaacaaaatc gttacacagc tttttaatta caattgataa agaattagaa aaattaaatg
      181 ttgagtttga tgaggcttat ctgcaattgc tcgaacttga aaactaaaat tgcaaccata
      241 ccattatggt taataccttt ttgttttttg tttttacaaa cagcagcaca gaataacgat
      301 tatgcaaacc ccaatgtaat aaggtatgag gattataact acaaccccaa tattaaaacc
      361 gtattattag aaggcgctac aggccaatta accgatgctg ctatcgaatt aaacaccaac
      421 cagcaattac tgctgcgttt cgatcatttg tatgccgatg ttgttgatta cagttatacc
      481 attatacatt gcgatgcaaa ttggcaaccc agccaaattt ccgaaagcca atatttgcaa
      541 ggttttagta ccgataaaat tttacgctat caatttgcca ctactacatt ggtgccttat
      601 gtacattatg agttgttgtt tccgaacgac ataataaaac ccattattag tggcaactat
      661 ttgctaaggg tatttaaaac cgatacacct gaaattaatg ttttaacaaa acgatttata
      721 gttttttcgc aactggcagg catacaggca aacatacatg caggcacttt tgccgataca
      781 cgctttacgc atcatgaaat agattttgaa ttgaatacac aacaactcaa tctgctaaat
      841 ccatataacg atttgcaagt agtgctttta caaaatgggg gctggctaca acgcatatat
      901 ggtttaaaac cccgctttgt aaatggcaat aaattggatt acaattatga tggcgaatac
      961 tgctttgccg gtggtaacga gttccggact tttgatacgc gcagtatcag gtttttaagt
     1021 gagcgcataa agcaaattac acaagccgat gataaaacgt ttattgtaaa cctgcacaac
     1081 gatatacgca cttcgtcaca acggtatgca tttgtaaatg atataaacgg ccaatattta
     1141 attaaacatc aggaaggccg aaacagtgat gttgatgccg attatgtatg ggtaaacttt
     1201 aaattaaaaa tgcctcagga agaaaccgaa ggcaacatgt acctgtatgg tgcgctcaca
     1261 aattggcgta ccgatacaac atcacttatg aattataatg ccgatgatat gagttatgaa
     1321 aaatcgttgc tactaaagca aggctattac aattatcaat atgcatacat aagcagtggc
     1381 agttaccccg atgttacagt tactgaaggc agtcattacg aaactgacaa cagttatacc
     1441 ttgttaattt actacagaaa ccccaatacg cgttatgatg ctattgtagg atataagaaa
     1501 ttaaacagca taaaaaagta aactcctaat ttaattgcct gcatcattcg tttatgctct
     1561 aaatcgttat tctttatttt tactatctat aaaaatagta acggggccat tgttacataa
     1621 tgtaatttgc atatcggcac caaaaatgcc ggtagcaggc ctatggccgg ttaattggaa
     1681 acaggtttct acaaatagtt cgtaaatggg aatggctatt tcgggtttgg cagctttaat
     1741 ataactgggc cggtttcctt ttttggttaa tgcatgtaac gtaaactgac ttacaattaa
     1801 aagccggcca ttagcctgta tcacatcttc attcattaca ccatttgcat catcaaaaat
     1861 gcgcagctta ataattttat tacacagcca ctgcacatcc tcagtagtat cggcatcttc
     1921 aataccaata aaaaccaaca aacctttttc tatttgccct gcaacctggt tgttaacatg
     1981 cacacttgca tgtgtaactc tttgaattaa agcgcgcatg ttttatttat ttaaacaaat
     2041 cttttccttc cataatgctt aggtaattat gatacctgtc gggcgaaatt ttaccggcta
     2101 tcaatgcatc tttaacagca catttgtttt cgttaatgtg caggcagtta ttaaatttgc
     2161 attgtggtaa aagtgcgcgc atttcaacaa aataatgcga tacttcctgt ggtaaaaaat
     2221 caaccgtacc taactcgcgt atgccgggcg agtcaattat aaaaccaccg gctttaagcg
     2281 gaaacatttc ggcaaaagtt gtggtgtgtt ttccggtttg gtgctgatcg gaaatagcac
     2341 cggtacgcaa ggttaatgtg ggttc
//
LOCUS       DAOMAA010000264         2364 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_41155_length_2364_cov_2.851018, whole genome shotgun sequence.
ACCESSION   DAOMAA010000264 DAOMAA010000000
VERSION     DAOMAA010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41155_length_2364_cov_2.851018"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="PLO59_04405"
     CDS             complement(<1..304)
                     /locus_tag="PLO59_04405"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HQV00377.1"
                     /translation="MKKSAFIFLLFISVSASAQKFAYIDTQYILDNIPDYKTAQQTLD
                     QISISWQKEIEGKYAIIDKLYKDYQAEQIILTEEMRKKREQEIVDKEKDVKELQKAK"
     gene            complement(459..>2364)
                     /locus_tag="PLO59_04410"
     CDS             complement(459..>2364)
                     /locus_tag="PLO59_04410"
                     /inference="COORDINATES: protein motif:HMM:NF018899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HQV00378.1"
                     /translation="TFTGNKAFSENKLKKLMKETKEKHIYHLFKTSKYIDENFEKDKE
                     KIIAKYLEKGYRDARIISDSVYKFDKNTVNLDINISEGNVYYIRNIEWLGNTKHSSKE
                     LALILGISKGDVYNQKQLESGLTQSQSGRDIQSLYNDEGYLFFQITPAEVKIENDSVD
                     LEMRIYEGKQATINRVIVKGNTKTNDRVIMRELRTKPGQLFSRTDIIRTQQEIAALGY
                     FNAEAMRIVPKPNPTDGTVDLEYTVEEKPSDQLELSGGYGGGRLVGTLGVSFNNFSAR
                     NMFNKEAWRPLPSGDGQRLSLRAQTSGVAYQSYSASFTEPYLGGRKPNSFTTSIYYSV
                     QNPSGYKRNDPRRSSIAILGTSVGLGKRLKFPDDFFQIYYEVAYQRYFVTNYGSAFLF
                     NNGTANAVSFTTNLSRNSIDAPVVQYPRRGSQVSLSLEATPPFSKFNDRNYTDLPDNE
                     KYKWIEYHKWKFNSSYFTKLTGNLVLNARVNFGFLGYYSKQIGAAPFERFYLGGDGLS
                     NFSLDGREIIALRGYENNSLTPYVGANQVGGTVYNKFTLELRYPVSLNPQAMVYGLAF
                     LEAGNSTIGVSTWSPFDNYKSAGVGVRIFLPMFGLLGLDWGYGFDKVPNQPNANGSNF
                     HFTIGQQFGN"
BASE COUNT          652 a          455 c          457 g          800 t
ORIGIN      
        1 actttgcctt ttgtagttcc tttacatctt tctctttatc tacaatttcc tgctcgcgtt
       61 ttttgcgcat ttcttcggtt aaaataattt gttcggcctg atagtcttta taaagtttat
      121 caatgatggc atatttacct tcaatttctt tttgccagga aatagaaatt tgatctaatg
      181 tttgttgtgc cgttttataa tcgggtatat tgtcgagtat gtactgcgta tcaatatagg
      241 caaacttttg ggcacttgcg ctaaccgaaa taaacagtaa aaagataaat gctgattttt
      301 tcatttttta ttttttattt tttatttttt aatagttgtg cattgctaat caaaccttgt
      361 gcaaattggc ggctacttgc tgaaattaat atgaatatgc atttgttggt ttttttcgga
      421 gggcgaatgt aatggtttgt gcagcattta aaaccctttt agttgccaaa ctgttgacct
      481 atggtaaagt gaaagttact gccattggca ttgggctgat tgggcacttt atcaaaacca
      541 taaccccaat ctaaacctaa taagccaaac atgggtaaaa atatgcgtac acctacacca
      601 gccgatttat aattatcaaa aggcgaccag gttgatacac caatggtcga atttccggcc
      661 tctaaaaatg ccaaaccata aaccattgcc tgagggttta acgataccgg atagcgtaac
      721 tctaaagtaa atttattata caccgtacca cctacctggt ttgcgcccac ataaggtgta
      781 agcgaattat tttcatatcc acgcaaagca ataatctcgc gtccatccaa tgaaaagttt
      841 gataacccat cgccacctaa ataaaacctt tcgaaaggag ctgcaccaat ttgcttacta
      901 taataaccca agaaaccaaa gttaaccctt gcgtttaaaa ccaaattgcc cgtaagtttt
      961 gtaaagtatg atgaattaaa tttccattta tgatattcaa tccatttata cttttcgtta
     1021 tcgggtaggt cggtatagtt gcggtcgtta aacttactaa atggaggtgt tgcctctaac
     1081 gataacgata cttgcgaacc tctgcgtggg tattgcacta ccggtgcgtc aatgctattg
     1141 cgcgataagt tagttgtaaa tgatactgca ttggctgtgc cattattaaa taaaaatgca
     1201 gagccatagt tggtaacaaa ataacgctgg taggctactt cgtagtaaat ctggaaaaag
     1261 tcgtcaggga attttaatcg tttacctaac cccaccgatg tacccagtat ggcaattgac
     1321 gacctgcgag ggtcgtttcg tttatagcct gatgggtttt gtactgaata ataaatggaa
     1381 gtggtaaacg aattgggttt gcggcctccc aaatagggtt cggtaaacga tgcactatac
     1441 gattgataag caacaccact ggtttgtgca cgcaggctta accgttggcc atcaccactg
     1501 ggtagtggac gccaggcttc tttattaaac atgttgcggg ctgagaagtt gttgaatgat
     1561 acgcctaaag tacctaccaa acggccacca ccataaccgc cacttaattc caactggtct
     1621 gatggttttt cttcaactgt atattctaag tcaacggtac catcggtagg attgggcttt
     1681 ggtacaatgc gcatggcttc ggcattaaaa taaccaagtg ccgctatttc ttgttgggtg
     1741 cgaattatat cggtacggct aaaaagttgg ccgggtttgg tgcgtaattc gcgcatgata
     1801 acacggtcgt tggttttggt atttcctttt acaattacgc ggtttatggt tgcctgtttg
     1861 ccttcgtaaa tacgcatttc aaggtcaaca gagtcgtttt ctatttttac ttcggcaggt
     1921 gttatctgaa aaaacaagta cccttcgtca ttataaagtg attgtatgtc gcggccactt
     1981 tggctttgtg ttaagccact ttcaagttgt ttctgattat aaacatcgcc tttgcttata
     2041 cctaaaatca aagccagttc ttttgacgaa tgtttggtat tgcccagcca ttcgatattg
     2101 cgtatatagt aaacgttacc ttctgatatg tttatatcga gattaactgt gtttttatca
     2161 aatttataaa cgctatcgct aataattctg gcatcacggt agcccttttc caaatacttg
     2221 gcaatgattt tttctttgtc cttttcaaag ttttcatcaa tatattttga ggtcttaaaa
     2281 aggtggtaaa tatgtttttc tttggtttcc ttcatcagct ttttaagctt attctcacta
     2341 aaagctttat tgccggtaaa ggtt
//
LOCUS       DAOMAA010000265         2359 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_41308_length_2359_cov_3.950087, whole genome shotgun sequence.
ACCESSION   DAOMAA010000265 DAOMAA010000000
VERSION     DAOMAA010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41308_length_2359_cov_3.950087"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2359)
                     /locus_tag="PLO59_04415"
     CDS             complement(<1..>2359)
                     /locus_tag="PLO59_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00379.1"
                     /translation="QGDVLWAQKISGTSIDKCLGVCSNGNNIVVVGTFESNLTIGTYT
                     VTSAGLNDVFIAQYDGNGTVLWLKSVGGPGNDFVADVVADTFGNICMAGSFVGTANFG
                     IAIFQSTINASVSTNDMFVSKLDSSGNFLWTKQAFGTGDDKATDIVTDANGNIFVCGQ
                     YSDTLTIDVVHPNILSNIACLFKLDANGSEQWFLPCISSSITPVALAVDALNRIAFTG
                     NFSGTLTTLSTPQQNIGGTYNNRIFVMQLDNNGTIQWIKSHSSTGLLRATNVKCTNTN
                     DVIILGEYHCRLNEFADIYGQGIFNSVGYSDIFVCKNKADGTLDWARCMGGPQNDFAA
                     GLALDTNNKIYFTGSFDKKFSAPFQNTTGCFTAGTAAVYCNDLNYSSYCQVTNGAIAS
                     GNRDIMYAQCIVTQRQPYDYYIRQSGNCQRDVIPACIIEQISQAACNDTSTICANNSA
                     YLSASARTDGNIGPAYTYQWSNGALSNILVTGGGTYTVTVTTEDGCFSSMADMTVTVV
                     TPPAYPLISDSKGVNNQATATQKVIVCSPDSVIIWASNLAPGSAYFWSVNGIVYYDDT
                     ITINQSALCSFTVGTNTQCPATNYIEVQVIQNTNSIHRINVINAVNDTVSNCSNNVHV
                     AFIDSVTSICYKDSVFWSITPMQSYNVGGFPCYSMATMSFIPDTNGWYIISCYGFNAQ
                     CGGDTVGPVSVKIYFEKLEVLPAQNIVIAGMRPICPVGDTITLTASGAINYTWAGPNI
                     IGNLSDSSIQITSIGTYTVSSAQLSTDSCVTYSSAGVLITTKTSPQ"
BASE COUNT          692 a          505 c          460 g          702 t
ORIGIN      
        1 tttgtggcga agttttagtt gtaatcaaca cacctgccga gctgtaggta acacaactat
       61 cggttgatag ctgagcactg ctaacagtat aggtccctat gctggtaatc tgaatactgc
      121 tgtcggataa attgccaatt atattagggc ctgcccatgt gtaatttatg gcaccagatg
      181 ctgtaagtgt tatggtatcg ccaacagggc aaataggccg catgccggca ataactatat
      241 tttgagcagg gagcacctct aatttttcga aataaatttt tactgaaact gggcctaccg
      301 tatcgccacc acattgtgca ttaaaaccgt aacatgaaat aatgtaccat ccatttgtat
      361 cgggtataaa tgacatggta gccatactgt aacacgggaa accgcccacg ttataagact
      421 gcataggtgt aatactccaa aacaccgaat ctttgtaaca gatgctggta accgaatcaa
      481 taaatgcaac atgcacattg ttactgcaat tgctaaccgt atcatttact gcgtttataa
      541 cattgatgcg atgtatgctg ttggtgtttt gaattacctg cacttcaata taattggttg
      601 cagggcattg tgtatttgta ccaacagtaa aactgcaaag ggcgctttgg tttatggtta
      661 tagtgtcatc ataatataca atgccattta cactccaaaa ataagcactg cccggagcta
      721 aattacttgc ccagataatt accgaatcgg gcgaacaaac aattactttt tgcgtagcag
      781 ttgcctgatt gttgacgcct ttactatcgc ttataagcgg gtatgccggt ggtgtaacta
      841 ccgttactgt catatcggcc atgctgctaa aacagccatc ttcagttgta acagttaccg
      901 tataggtgcc accaccggta actaaaatat tacttaatgc accgttactc cattgatagg
      961 tataagcagg cccaatgttt ccatcggttc gtgccgaagc gcttaaatat gccgaattat
     1021 tagcacaaat ggttgacgta tcgttacaag cggcttgcga tatttgttca ataatgcatg
     1081 ccggtattac atcacgctgg cagttgccac tttgtcgtat gtaatagtcg taaggctggc
     1141 gttgggttac aatgcactgt gcatacataa tatctctgtt gccacttgca atggcaccat
     1201 tggtaacttg gcagtagctg ctgtagttta aatcgttgca atacacggct gccgtgcctg
     1261 cagtaaagca accggttgta ttttgaaaag gggccgaaaa ttttttatcg aaacttcctg
     1321 taaaataaat tttattgttt gtatctaaag caaggcctgc agcaaaatca ttttgtgggc
     1381 cgcccatgca gcgtgcccaa tctaatgtac catctgcttt atttttacat acaaaaatat
     1441 cactgtaacc tacactgtta aaaatacctt gtccgtatat atctgcaaac tcattaaggc
     1501 gacaatggta ttcgcctaat ataattacat catttgtgtt ggtgcacttt acattagttg
     1561 cacgcaataa accagtgcta ctgtgcgatt ttatccattg tatagtgcca ttattatcaa
     1621 gttgcattac aaaaattctg ttgttgtaag tgccgccaat attttgttgt ggcgttgata
     1681 aggttgtaag ggtgccgcta aaattaccgg taaatgcaat acggtttaat gcatcaacgg
     1741 ccaatgctac cggagttatt gatgatgaaa tacaaggcaa aaaccattgt tcgctaccat
     1801 tagcatctaa tttaaacagg caagcaatat tgctcaggat gttgggatgt accacatcaa
     1861 tggtaagcgt atcgctatac tggccgcaaa caaaaatgtt accgttggca tctgtaacaa
     1921 tatcggtggc tttatcatct ccggtgccaa atgcttgttt agtccataaa aaattgccgc
     1981 tactgtctaa cttcgaaaca aacatgtcgt tagtacttac actggcattg atggtacttt
     2041 gaaaaatggc aatcccaaag ttggcagtgc ctacaaagct accggccata catatattac
     2101 caaatgtatc ggcaactaca tcagctacaa aatcgttgcc aggcccacca acacttttta
     2161 gccacaacac agtgccattg ccgtcatact gtgctataaa aacatcatta agccctgccg
     2221 atgttaccgt ataagttcca atggttaagt tactttcaaa agtaccaact actactatgt
     2281 tgttgccgtt actgcaaaca cccaaacatt tatcaatact ggtaccacta attttttgcg
     2341 cccataaaac atcgccctg
//
LOCUS       DAOMAA010000266         2359 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_41336_length_2359_cov_2.301649, whole genome shotgun sequence.
ACCESSION   DAOMAA010000266 DAOMAA010000000
VERSION     DAOMAA010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41336_length_2359_cov_2.301649"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="PLO59_04420"
     CDS             complement(<1..742)
                     /locus_tag="PLO59_04420"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HQV00380.1"
                     /translation="MKWTIWIFALMLTLNINAQSNITIQTALGAISAIYPNFPKSDKS
                     TFSVAVQLNKQWRGNKSWHSYYRYPTLGLGIYYLESGSGALGQSVALCPQLGFKQSIN
                     GKLSLFENGGLGLAMFNKPYNEINNPQNIAVGSKLTAFATAQIGFIYQYNSKLNILAF
                     GGLMHYSNAHVQLPNVGLNVFNGGIGVKYFFKNNSSLLPDTTKHFDKKWHFNLKVALG
                     INEQGSSIIAVNGPKYPIYIASVFLARKF"
     gene            complement(739..1503)
                     /gene="tpiA"
                     /locus_tag="PLO59_04425"
     CDS             complement(739..1503)
                     /gene="tpiA"
                     /locus_tag="PLO59_04425"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016775675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HQV00381.1"
                     /translation="MAHKIAAANWKMNGDLNFVKQLTQELLANQTIINSNQSLLLASP
                     FLYLHELVEATKNYKQINIAAQNCAQYTSGAYTGEVSADMLASIGVTHVIIGHSERRA
                     LFGETDAQLAIKVLLAIQNKLKPVFCIGETLAQREANKHFNIIEDQLWHGLFQLSEAD
                     FSQCIVAYEPVWAIGTGVTATPEQAQEMHAYIRGLIAEKYNDDVAQATSILYGGSCNE
                     KNAATLFSLPDVDGGLVGGASLKAESFAQIVAALNK"
     gene            complement(1509..1955)
                     /locus_tag="PLO59_04430"
     CDS             complement(1509..1955)
                     /locus_tag="PLO59_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00382.1"
                     /translation="MKKIFLLLYGCIITTLCYSQNKAAILLTDLKVQVGQQSLIGPDV
                     QTNMTMGKSTDSYILFRNDSILITAVYKLTAFSSRRSELKDGTLKLKIEYECKYLSTS
                     TKTKVERMYYLDDKLMLNEKQLLNVKLSKFNSKQLYISYTGKITPQ"
     gene            2024..>2359
                     /locus_tag="PLO59_04435"
     CDS             2024..>2359
                     /locus_tag="PLO59_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010855307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00383.1"
                     /translation="MTNTIRVLAITFSIAFFVMVGYSFKPAPESTYTYRQFSTIESVV
                     PGGLGRSRILATDASGTSVEKDLMNFYSFTGINFGNVTNNDKLIVDKINEYTADGWEL
                     CQVTSGVQSS"
BASE COUNT          717 a          439 c          415 g          788 t
ORIGIN      
        1 taaatttacg agccagaaaa accgaggcaa tataaattgg gtacttgggg ccattaactg
       61 caattatgga agagccttgc tcatttattc ccaatgcaac ttttaaatta aagtgccatt
      121 ttttgtcaaa atgtttagtg gtatcgggta ataaactgga attattttta aaaaaatatt
      181 tcacaccaat acctccatta aaaacgttta aaccaacatt gggtaattgc acatgtgcat
      241 tgctgtaatg cattaagccg ccaaatgcaa gtatgtttag ttttgagttg tattgatata
      301 tgaaacctat ttgtgcagtt gcaaatgccg taagtttgct tcctactgca atgttttgtg
      361 gattattaat ttcattataa ggtttattaa acattgccaa acccaaacct ccattttcaa
      421 ataatgaaag tttgccgtta atgctttgct taaaacctaa ttgcggacac agtgcaaccg
      481 attggcctaa agccccgcta cccgattcta aataataaat acccaaacct aatgtaggat
      541 aacggtagta gctatgccac gatttgtttc cacgccattg cttattcaat tgcactgcaa
      601 cagaaaaagt acttttatca gatttcggaa aattagggta tatagccgag atagcaccta
      661 gtgccgtttg tatggtaata ttagattgcg catttatgtt taacgtaagc attaatgcaa
      721 aaatccaaat agtccacttc atttatttaa tgcggctaca atctgggcaa aactttctgc
      781 ctttaatgat gcccctccaa ccaaaccacc atcaacatcg ggcaatgaaa acaatgtagc
      841 tgcatttttt tcattacaag atccaccata taaaattgaa gttgcctgtg caacatcatc
      901 gttatatttt tcggcaatta aacctcttat gtatgcatgc atttcttgtg cttgctccgg
      961 tgtggcagta acaccggtac caattgccca cacgggttca taggcaacaa tacattgact
     1021 aaaatcagct tcacttaatt gaaacaagcc atgccaaagc tggtcttcta ttatattaaa
     1081 atgtttgttg gcttctcttt gagccaaggt ttcgccaata caaaaaacag gcttcagttt
     1141 attctgtatt gccagcaaaa cttttatggc caactgcgca tcggtttcac caaatagggc
     1201 tctgcgctcg ctatgtccaa taattacatg cgtaacgcca attgaagcaa gcatatcggc
     1261 acttacttcg cctgtataag ctcctgaagt atattgcgca cagttttgag cagcaatatt
     1321 tatttgctta taatttttag ttgcttcaac caactcatgc aaataaagaa atgggcttgc
     1381 cagtaataaa ctttggttgc tatttataat tgtttgatta gccaataatt cttgtgtaag
     1441 ctgtttcaca aaatttaaat cgccattcat tttccagttt gctgctgcta ttttatgcgc
     1501 cataattttt attgtggagt tattttaccg gtataagaaa tataaagttg tttgctgtta
     1561 aacttgctca gttttacatt gagtaattgc ttttcattta gcatcagttt atcgtctaaa
     1621 taatacatgc gttcaacttt agttttggtt gaagttgaaa ggtatttaca ttcatattca
     1681 atttttaatt ttagtgtgcc gtcttttaat tcgcttctgc gactactaaa agctgtgagt
     1741 ttataaactg ccgttattaa aattgaatcg tttcgaaaca aaatataact atccgttgat
     1801 ttgcccatag tcatattggt ttgcacatcg ggcccgataa gcgactgttg cccaacctga
     1861 acttttaaat cggttagcaa tatggcagcc ttgttttgac tataacataa agtagtgatt
     1921 atacatccgt ataaaagcaa aaatattttc ttcatgttag tttaaattgt tttgtttggg
     1981 caaatataaa gaagcaaatt aaaccctatt aactttaaaa caaatgacaa acacaattag
     2041 agtattggct atcacattca gcattgcatt ttttgtaatg gttggctaca gttttaaacc
     2101 ggcacctgaa agcacttaca cctacaggca gttttctact atcgaatcgg tggtgccggg
     2161 tggcttaggt cgttcgcgca ttttagctac tgatgcaagt ggcacatcgg ttgaaaaaga
     2221 tctgatgaac ttttacagtt ttaccggtat taactttggt aatgtaacca ataacgataa
     2281 gttgattgtt gataagataa acgaatatac agccgatggc tgggagttat gtcaggttac
     2341 atcgggcgtg caaagttcg
//
LOCUS       DAOMAA010000267         2352 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_41520_length_2352_cov_3.109273, whole genome shotgun sequence.
ACCESSION   DAOMAA010000267 DAOMAA010000000
VERSION     DAOMAA010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41520_length_2352_cov_3.109273"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1136)
                     /locus_tag="PLO59_04440"
     CDS             complement(<1..1136)
                     /locus_tag="PLO59_04440"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HQV00384.1"
                     /translation="MWLALLIFIGINTAKADEGMWLLSKLKQINEAEMKGLGLKLSAD
                     DIYNINQSCLKDAIVQMGSVSGNSFGGFCTAEIVSDQGLVLTNHHCGLDAIQENSSVE
                     HDYLTNGFWAMSKADELPNPGLGVRFLVRMEDVTQEVLTQLSDTMSEATRNAAITKAI
                     AGITKKAAEGNNYLADVRGFYRGSEFYLFIYEVFKDVRLVGAPPYAIGNFGGDTDNWV
                     WPRHTGDFSMFRIYANAENKPAEYAATNVPYKPKKFLEVSKKGMTENDYAMIIGFPGR
                     TDRYATSHSLKMALEITNPAIVDIRKAKLDVINKAMDENDKVRIQYQDKKNQISNYWK
                     YYIGQTAQLKRNKVVETKTDLENQFANWANANASRKAQYGNLLS"
     gene            complement(1286..>2352)
                     /locus_tag="PLO59_04445"
     CDS             complement(1286..>2352)
                     /locus_tag="PLO59_04445"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HQV00385.1"
                     /translation="IHRGVHYLSQIASQAYDDVRIKTQKFLGAAQASEIVFVRGTTEA
                     VNLVAATYGRQQLKAGDEILISHMEHHSNIVPWQILAQQTGAVLKVIPINKNGELDMQ
                     AFYVLCTAKTKIVSVTYVSNALGTINPVKEIIDNAHKQGAVVLIDGAQAVSHFDLDVQ
                     ELNCDFFACSAHKMLGPTGIGVLYGKHHLLTQMPPYMGGGDMIQSVSFNGTTYNEVPY
                     KFEAGTPHIEGVIAWGAALDYWQTINRAQAMQHEQSLLNYCTNQLQQIKRVKIIGTAA
                     NKCSVCSFVIEGVSNFDAGMFLDTMGIAVRTGQHCTEPLMDALGVKGTIRASFFFYNT
                     IAEVDKLIAAVKKCIDVLAK"
BASE COUNT          622 a          500 c          448 g          782 t
ORIGIN      
        1 ttgctgagca agttgccata ttgtgcctta cggcttgcat ttgcattagc ccaatttgca
       61 aattggtttt ctaaatcggt tttggtttct acaactttat tgcgtttaag ctgtgctgtt
      121 tggcctatgt aatatttcca atagtttgat atctgatttt ttttatcctg atactgaatg
      181 cgtactttat cgttttcatc catggcttta ttaatcacat ctaatttggc tttgcgtata
      241 tcaacaattg ccggatttgt aatttctaat gccattttta aactgtggct ggtagcatac
      301 ctgtcggtac ggccagggaa accaataatc atggcgtaat cattttcagt catgcctttt
      361 ttacttacct ctaaaaactt tttgggttta tagggcacat tggttgctgc atattcggcc
      421 ggtttgtttt cggcattggc gtatatccga aacatcgaaa aatcgcctgt atggcgtggc
      481 cacacccagt tatcggtatc gccaccaaag tttccgatag cataaggtgg tgcgccaacc
      541 aaacgcacgt ctttaaacac ttcgtaaata aaaagataaa actcgctgcc tctgtaaaag
      601 cctcttacat ctgctaaata attattgcct tcggctgctt ttttggtaat tccggcaatg
      661 gcttttgtaa tggctgcatt gcgagtggct tcgctcatgg tatcgcttaa ctgcgttaat
      721 acctcttgcg ttacatcttc catgcgtact aaaaaccgta cacctaagcc cgggtttggc
      781 aactcatcgg cttttgacat ggcccaaaat ccattggtta aataatcgtg ttcaactgag
      841 ctgttttctt gtatggcatc taagccacag tgatggttgg taagcaccag accttggtcg
      901 cttacaattt cggcagtaca aaaaccgcca aatgaattgc cgcttacgct gcccatttgt
      961 acaatggcat ctttaaggca cgattggttg atgttgtaaa tatcatcggc acttaacttt
     1021 aagcccaagc ctttcatttc ggcttcgttt atttgcttta gtttgctaag caaccacatg
     1081 ccttcatcgg ctttagcggt atttatgcca ataaaaataa gcagtgctaa ccacatgctt
     1141 cggtttgctg taaacatatt taaatggttt taggagttaa cagtacaaag aaagttttaa
     1201 aattatttac caaatactta ctcgggttaa gtacatgtta acagctatcg tttatacgaa
     1261 gcaagtagtt attgttttgc aaaaactatt tggcaagcac atcaatacac tttttaacgg
     1321 ctgcaattag tttatcaact tcggctatgg tattgtaaaa gaaaaacgaa gctcttatgg
     1381 tacctttaac gcccaaagca tccattaagg gttcggtaca gtgttgcccg gttcgtacag
     1441 ctatgcccat ggtatctaaa aacatacccg catcaaaatt gctaacaccc tcaataacaa
     1501 atgagcatac actgcatttg ttagcagcgg tgccaattat cttaacccgt ttgatttgtt
     1561 gaagctggtt ggtgcaataa tttaaaagtg attgttcgtg ttgcattgct tgcgctctgt
     1621 ttatggtttg ccaataatcc aatgcagcac cccatgcaat aaccccctct atatgcggag
     1681 ttccggcttc gaatttatat ggcacttcgt tgtaggtggt tccattaaac gatacggatt
     1741 gaatcatatc gcctccaccc atatatggtg gcatttgcgt aagcaggtgg tgtttaccat
     1801 aaagcacacc tatgcctgtt gggcctaaca ttttatgcgc actgcaagca aaaaaatcgc
     1861 agttaagttc ttgtacatct aaatcgaaat gcgatacagc ttgtgcacca tcaattaaaa
     1921 ctactgctcc ttgtttatgc gcattatcaa taatttcttt taccgggtta atggttccta
     1981 atgcattgct tacataggta accgaaacta ttttagtttt ggctgtacac aaaacataaa
     2041 acgcctgcat atctaattcg ccatttttat ttatcggaat tacttttaaa acagctccgg
     2101 tttgttgcgc taaaatttgc cagggtacaa tgttgctgtg atgttccatg tggctaatta
     2161 atatctcatc acccgccttt agttgttggc ggccataagt agcagctacc aaatttacgg
     2221 cctctgtagt gcctcgtaca aaaacaattt cgctggcttg tgctgcacct aaaaattttt
     2281 gtgttttaat cctaacatca tcgtatgcct gcgatgctat ttggctcagg taatgtacac
     2341 ctctgtgaat gt
//
LOCUS       DAOMAA010000268         2351 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_41562_length_2351_cov_3.381098, whole genome shotgun sequence.
ACCESSION   DAOMAA010000268 DAOMAA010000000
VERSION     DAOMAA010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41562_length_2351_cov_3.381098"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            10..201
                     /gene="rpsU"
                     /locus_tag="PLO59_04450"
     CDS             10..201
                     /gene="rpsU"
                     /locus_tag="PLO59_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015027473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HQV00386.1"
                     /translation="MLIVPIKENESIDKALKKFKKKFEKTGAVKQLRSRQAFEKPSVA
                     RRTEVKKAVYKAQMNRSED"
     gene            373..1245
                     /locus_tag="PLO59_04455"
     CDS             373..1245
                     /locus_tag="PLO59_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HQV00387.1"
                     /translation="MSYLTFEKRYSAHTILAYQTDLAQFFIYVEAQYQTVDITAINHF
                     IIRSWVVSLMEAKLNPRSVNRKITVLKTFYKFLLLQGLVSNNPMEKIVAPKTAKRLPV
                     FVDKPKMNKVLDVQHFSDDFLGKRNRLILELFYATGVRLSELCAVTLNQISLDSLQIK
                     VLGKRNKERIIPFNQHLIPLLHDYLQMRKQVTGSLQTEINQLLITDKAKPVTGKQIYH
                     IVRNSLGQHTTLQKRSPHVLRHTFATHLLDNGADINAIKELLGHSSLAATQVYTHNTI
                     EKLKNIHKQAHPRN"
     gene            1337..1636
                     /gene="raiA"
                     /locus_tag="PLO59_04460"
     CDS             1337..1636
                     /gene="raiA"
                     /locus_tag="PLO59_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HQV00388.1"
                     /translation="MKLKVHSIHFDADKKLIAFVEEKVDKLLTFFDHIIDGEVYLKLD
                     KAENSKNKIAEIKLHAPGKTLFAKEQCVSFEEATDLAVDALRKQLSKHKEKVKNL"
     gene            complement(1669..2043)
                     /locus_tag="PLO59_04465"
     CDS             complement(1669..2043)
                     /locus_tag="PLO59_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00389.1"
                     /translation="MHTQQQFQSLQDEHHEWLAKLQFYKSDLAQHEQVLEQKSVLAQS
                     TDAKAMIEHFQNQFILQREVLDILRHDIKQFENELVHLQQHGVDKTKQGIQMLKDSVF
                     DQLQTFEKLYHDLRHEFVSYAQ"
     gene            complement(2070..>2351)
                     /locus_tag="PLO59_04470"
     CDS             complement(2070..>2351)
                     /locus_tag="PLO59_04470"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV00390.1"
                     /translation="ISNTISITTGITDLTSNILMISPNPASNAFSIVVHAVVGSTDKF
                     IVINNLGQVVLEQALLEKNVINSANWPSGIYAVRCFIGDKTIVKSIIIR"
BASE COUNT          800 a          415 c          387 g          749 t
ORIGIN      
        1 aaaaatcaaa tgctaatagt tcctatcaaa gaaaacgagt cgatagacaa ggcccttaaa
       61 aaatttaaga agaaattcga aaaaaccggt gcagttaagc aattacgctc gcgtcaggct
      121 ttcgaaaaac catctgttgc tcgtagaact gaggttaaaa aagccgttta caaggctcaa
      181 atgaaccgca gcgaagacta aacatatacc tgaataaggt ttcccagata tgcatcggca
      241 actgaattat ttcggttgcc gatttttttt ttcactacgc ctcaataaat tttataaaaa
      301 gttgttattg attgggcata ttaaattgta catttactac gtaagataaa gccgatgatt
      361 actgaaaagt ttttgagtta cctcactttt gaaaaacgat attcggcaca taccatactt
      421 gcctatcaaa ccgatttagc acaatttttt atttatgtcg aagcgcaata tcaaacggtt
      481 gacataactg caataaatca ttttataata cgctcgtggg ttgtaagttt gatggaggca
      541 aaattaaatc cacggtctgt aaaccgaaaa ataaccgttt taaaaacatt ttataaattt
      601 ttgctgttgc agggtttggt gagtaataac cccatggaaa aaattgttgc ccctaaaaca
      661 gctaaacgat tgccggtttt tgtagataaa cctaaaatga ataaggtgct tgatgtgcag
      721 cattttagtg atgacttttt aggcaaacgc aacaggttaa tactggagtt attttacgcc
      781 accggtgtac gcctaagcga actttgtgca gtcacactta atcaaatttc gttagatagc
      841 ctgcaaataa aagttttggg caaacgaaac aaagagcgca ttattccatt taatcaacat
      901 ctgataccct tgctgcatga ttacttacaa atgcgtaaac aagttacagg cagtttacaa
      961 acagaaatca atcagctttt aataaccgat aaagcaaagc ctgttacagg aaaacaaatt
     1021 tatcacattg tacgaaacag tttagggcaa cataccacct tgcaaaagcg aagcccacat
     1081 gtgttgcgtc atacttttgc cacacattta ttagataatg gtgctgatat aaatgccata
     1141 aaggaattat tgggtcatag cagtttagct gcaacacagg tttatacgca taatactata
     1201 gagaaattaa aaaacatcca taaacaagcg catccgcgca actaacattt ttatttatta
     1261 atagttaaaa catatgtaga atttacattt tatttgaaat ctctctttaa ttatttaacc
     1321 cttcaattta ttagttatga aattaaaagt gcacagtatt cattttgatg ccgacaaaaa
     1381 gcttattgct ttcgtagaag aaaaagtaga taagctcctt accttcttcg atcatattat
     1441 tgatggcgaa gtttatttaa aattagataa ggccgaaaac tcgaaaaata aaatagcaga
     1501 aattaaactg catgctcccg gcaaaacact ttttgcaaaa gaacaatgcg taagttttga
     1561 agaagcaacc gatttggcag ttgatgcctt gcgaaaacaa ctatcgaagc ataaagaaaa
     1621 agttaaaaat ttataattaa aatgcccgcc aatagcgggc tttttaattt attgtgcata
     1681 gcttacaaat tcgtgccgta agtcatggta cagtttttca aaggtttgta actgatcaaa
     1741 cacactatcc tttaacatct gaataccctg tttggtttta tcaacaccat gttgttgtaa
     1801 gtgtaccaat tcgttttcaa actgcttaat atcatgcctt aaaatatcca atacttctct
     1861 ttgcagtata aactgatttt ggaaatgctc tatcatcgct ttagcgtcag tactttgtgc
     1921 taaaacactt ttttgttcaa gtacttgttc atgctgtgcc agatctgatt tataaaactg
     1981 cagtttggct aaccattcat gatgctcatc ttgtaaagat tgaaattgtt gctgggtgtg
     2041 catggcttta atatttagag tttaaacttt tatcggatta ttattgattt tacaattgtt
     2101 ttatcaccaa taaaacatct tacagcataa attcctgatg gccagtttgc cgaattaatt
     2161 acattttttt cgagcaaagc ctgctctaaa actacttgtc ctaagttatt tataacaata
     2221 aatttatctg tactaccaac cacagcatgt accacaatag aaaatgcatt gcttgccgga
     2281 ttggggctta tcatcaatat attacttgtt aagtcagtta tacccgtagt tatactgatt
     2341 gtgtttgaaa t
//
LOCUS       DAOMAA010000269         2348 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_41656_length_2348_cov_3.608809, whole genome shotgun sequence.
ACCESSION   DAOMAA010000269 DAOMAA010000000
VERSION     DAOMAA010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41656_length_2348_cov_3.608809"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2348)
                     /locus_tag="PLO59_04475"
     CDS             complement(<1..>2348)
                     /locus_tag="PLO59_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00391.1"
                     /translation="YQFAIINNEGMVMYHSDAMRNMQENILTETENNTVLRSMLFSRE
                     SGCFQSLYHGKMHSFYIKPISQLPLNILVFNDNRQQSDVVLYAFCFTFFMLAILLGIN
                     MLEVLLIGALVTQSTKLKRNYQVFDTLFPNANHIKLYNLFCKYFLLAIALCIVSFVFD
                     SFINSVFMLFMSINLCSILIQKSYMDLQPDEWQFKGRLKRLWLLAVIAIVYNVIAFIT
                     SMHVWLLWLLQVILFTAGIWLLNVTRAPSVANPRQQKFLHYLNILWPFNYIQSYSTMI
                     MLRLILIGVLPVIFFYINAYNNEMRINTRMDQVSIIKELEQKNIRSISNNKQVNPDNT
                     YSYLDSLTNIKWINASTTKQKTEPCNVNEKFFALFRLQLNNNRLYNLAFCQTEDGNSN
                     LFFRNQGLTQIATQNTRSNDVIQVSTANIGFFKCPGAITTILIFCTAALMLIALYALL
                     KVGVRKLFSLNQTDVLRFSQLNLDTICDKKNRLIFVNGSPGSNKFSTIHTALLGKACY
                     NASEIGVINFIKIPDSDNDRLANYEWNNQIQKIKSGNTKLVILTHFEYANADERINRI
                     KLNLLEDLLVNIDKDNERFRIIIISTVHQIAFLDFIQKDFATTVFNNDMKRWSILLGQ
                     FVNVFLPLKENCMAIHVDDMMQKDNRCVTNADTNQKQLLSLMADECRYTSFLDNQKVE
                     LANHILHTKSVRDPEELILYIQNAAYLYYRQLWDTLTFEEKFIVLDMAEDGLANTTDS
                     HHVNVLLCKRILLFKNGRLTLFNTSFRNFILTAIEKDEIQAL"
BASE COUNT          751 a          397 c          404 g          796 t
ORIGIN      
        1 taagtgcttg tatctcgtct ttttctatgg ctgttaaaat gaaattgcga aaactggtat
       61 taaacaaggt aagccgtcca tttttaaaaa gcaaaatgcg tttgcacaat aaaacattaa
      121 catggtggct atctgttgtg tttgccaatc catcttcggc catgtctaaa acaataaatt
      181 tttcttcaaa ggttaaggta tcccataact gccggtagta taaatatgct gcattttgaa
      241 tatatagaat aagctcttcg gggtctctta ccgattttgt atgtaaaatg tggttggcta
      301 attcaacctt ttgattgtct aaaaaactgg tatatctgca ctcatcggcc ataagggata
      361 aaagttgttt ttgattggta tcagcattag taacgcacct gttatctttt tgcatcatgt
      421 catccacgtg tatagccata cagttttctt taagtggcag aaatacattt acaaattgac
      481 caagcaatat gctccaacgt ttcatatcat tattaaatac ggtagtagca aagtcctttt
      541 gtataaaatc taaaaaggca atttgatgta cggttgaaat aattataatc cgaaaccgct
      601 cattgtcttt atcaatattt actaataaat cttctaacaa gtttagctta attctgttaa
      661 tccgttcatc ggcattggca tattcgaaat gtgtaagaat tacgagtttg gtattacctg
      721 atttgatttt ttgtatctga ttgttccatt catagttggc taaacggtcg ttatctgagt
      781 cgggtatttt tataaaattt ataaccccaa tttcagaagc attgtaacaa gcttttccta
      841 ataaagcagt atggatggtc gaaaatttat tcgaccccgg actaccattt acaaaaatca
      901 gcctgttttt tttatcacaa atggtatcta aatttaattg gctaaacctg agcacatctg
      961 tttgattgag tgaaaaaagt ttgcgaactc ccacttttag caatgcatat aaggcaatta
     1021 gcatcaatgc tgccgtgcaa aaaattaaaa tggttgtaat tgcacccggg catttaaaga
     1081 aaccaatgtt agcggttgaa acttggataa catcgtttga tctagtgttt tgtgtagcta
     1141 tttgtgtaag gccttggttt cgaaaaaaca ggttgctgtt accatcctct gtttggcaaa
     1201 atgctaaatt gtataacctg ttattgttaa gctgtaacct gaacaaggca aaaaactttt
     1261 catttacatt acatggttct gttttttgtt tggttgttga tgcattaatc catttaatat
     1321 ttgttagcga gtctaaatag ctgtaagtat tatcaggatt tacttgttta ttatttgaaa
     1381 tgctacgtat gtttttttgt tccagctctt ttataataga cacctgatcc atacgggtgt
     1441 ttattcgcat ttcattattg tatgcgttta tgtaaaaaaa tattacaggc agtacgccaa
     1501 ttaaaatcaa ccgcaacata atcatggtgc tatagctttg tatgtaattg aaaggccata
     1561 aaatatttaa gtaatgtaaa aatttttgct gccttgggtt ggctacactt ggcgccctag
     1621 ttacatttag taaccaaatg ccggctgtaa ataaaattac ctgaagtaac catagcagcc
     1681 atacatgcat gcttgtaata aatgcaataa cattatatac aattgcaatt acagccaaca
     1741 accacaacct tttcaaacgg cctttaaatt gccactcatc gggctgtaag tccatataac
     1801 ttttttgaat cagaattgag caaagattga tgctcataaa aagcataaaa acactgttga
     1861 taaacgaatc gaatacaaac gatacgatac ataatgcaat agccaataaa aagtatttac
     1921 aaaacaggtt gtaaagttta atatgatttg cattaggaaa taatgtatcg aaaacttgat
     1981 agttgcgttt tagctttgtt gactgtgtta ccaacgcccc aattagcaac acctcgagca
     2041 tatttatacc taataaaatt gccagcataa aaaaagtaaa acagaaagca tataatacaa
     2101 catcgctttg ctgcctgtta tcattaaaca ccaaaatatt tagcggtaat tggctaatcg
     2161 gttttatata gaaactgtgc attttgccat gatacagaga ttggaagcag cccgactcgc
     2221 gcgaaaaaag catacttctt aatactgtat tgttttcggt ttcggtaagg atgttttcct
     2281 gcatgttgcg cattgcatct gagtggtaca ttaccatgcc ttcgttgtta ataatggcaa
     2341 attgatag
//
LOCUS       DAOMAA010000270         2345 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_41761_length_2345_cov_3.006114, whole genome shotgun sequence.
ACCESSION   DAOMAA010000270 DAOMAA010000000
VERSION     DAOMAA010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41761_length_2345_cov_3.006114"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="PLO59_04480"
     CDS             <1..351
                     /locus_tag="PLO59_04480"
                     /inference="COORDINATES: protein motif:HMM:NF014822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HQV00392.1"
                     /translation="RTFYAALLSVCNNNEAVQKGKFYELQEAQKGNIAYNDSLMYAFA
                     RFTPNEKVIVVCNFQNKNFDGTIQLPEVLKSALHVSEKTDAIDLLSGAKFKCNTSGLH
                     LKLPGMSAVIIRLY"
     gene            373..1698
                     /locus_tag="PLO59_04485"
     CDS             373..1698
                     /locus_tag="PLO59_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016400196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQV00393.1"
                     /translation="MSNTDKPLLKFSQIFNMSFGFLGIQFGFALQNSNVSRIFQTLGA
                     NQDEIPILWIAAPVTGLLVQPIVGHISDSTWHKRWGRRRPFFFLGALLAAFALIFMPN
                     SNALWIAGALLWLMDASINISMEPFRAFVGDMLPSRQRTLGFATQSFFIGIGAVVASM
                     LPWMFTNWFLVSNTAAEGIIPDSVKYSFYIGAIVFLMAVLYTVFTTKEYEPGTHGMPP
                     LDEYKPSIAQSLADSFKGVMQMPKTMKQLAVVQFFTWYALFCMWIYTTTAVTSSIYKT
                     SDTTSAAYNEGADWVGICFAAYSGVAALVAFFLPPLAKLFSRRITYIICLISGAAGLV
                     SIAYIQNPDLLLLSMIGVGVAWAGILSMPYAILAGSLPAGKMGYFMGVFNFFIVIPQI
                     VASFSLGPILKYVFNNEAQYMLLLGGLSMIIAAVICTRVQDSDDKKLGV"
     gene            complement(1700..>2345)
                     /locus_tag="PLO59_04490"
     CDS             complement(1700..>2345)
                     /locus_tag="PLO59_04490"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQV00394.1"
                     /translation="EHKPTSSRLRFLYDNHIIQDVKQKKVGPEKTVEYYSIAETQLKK
                     CIEIHPDYFEAYFGLGDLYVQRNDMKQAMYYYKTIVQRMPNLALGYNNLGNMYFKVQQ
                     YDSAITVLQKAIVLMPDYADAYNNLGSAYFGKGDYNNAIKYYLKTIELSPNYVDAWKN
                     LGSSYGSIKDYDKSIDAFSNALSLSPNDADIKRFLDLTINFKNQAAATTGMPTN"
BASE COUNT          656 a          404 c          474 g          811 t
ORIGIN      
        1 cgaacttttt atgcagcatt gctaagtgtt tgcaataata atgaagccgt acaaaaggga
       61 aaattttatg aacttcaaga agcccaaaaa gggaatatag cgtataacga ttcgttgatg
      121 tatgcttttg cgcgttttac acccaatgaa aaagtgattg tggtttgtaa ttttcagaat
      181 aaaaattttg atggcaccat acagttaccc gaggttttaa aatcggcttt acacgtttct
      241 gaaaaaaccg atgctataga tttattatcg ggcgctaaat ttaagtgtaa tacaagtggc
      301 ttgcatttaa aacttcccgg aatgagtgcc gtaataatca gattatatta agcttttaat
      361 tagataaact ttatgagcaa cacggataaa ccactattaa agttttcgca aatctttaat
      421 atgagctttg gtttcttagg cattcaattt ggttttgcac tgcaaaactc aaatgtaagc
      481 cgcattttcc aaacattggg tgcaaaccag gatgagatac ctatattatg gattgctgca
      541 ccggttaccg ggttgttagt acagcccatt gttggccata ttagcgatag tacctggcat
      601 aagcgatggg gcagacgcag accttttttc ttcttagggg cattattggc tgcatttgca
      661 ttaattttta tgcctaactc caacgcattg tggattgccg gtgctttatt atggttaatg
      721 gatgcatcca ttaatatttc gatggagcca tttagagctt ttgttggtga tatgctgccc
      781 agcaggcaac gcacattggg ttttgctaca caaagttttt ttattggcat tggtgctgtg
      841 gtagcaagta tgttgccctg gatgtttacc aattggttcc ttgtaagtaa tacagcggcc
      901 gaagggatta ttcccgattc ggtaaagtac tcgttttata taggtgcaat tgttttttta
      961 atggctgtat tgtacaccgt ttttacaact aaagagtatg aaccgggcac ccatggtatg
     1021 ccacctttgg atgaatataa accaagtata gcacagtcgt tagccgattc gtttaaaggt
     1081 gtaatgcaaa tgcccaaaac catgaaacaa ttagcagtgg tacagttttt tacctggtat
     1141 gctttgtttt gtatgtggat ttatactacc acagcagtaa ccagcagcat atataaaacc
     1201 agcgatacta cttcggctgc atataatgaa ggtgccgatt gggtgggcat ttgttttgct
     1261 gcttatagtg gtgttgcagc attagttgca ttttttttac cgccattggc aaaattattt
     1321 tcgcgaagaa taacttacat catctgcctt ataagtggtg ctgccggatt agtttctata
     1381 gcctacatac aaaaccccga tttattgcta ttgagcatga taggtgtagg tgtggcatgg
     1441 gcaggtattt tatcaatgcc ttacgccata cttgccgggt ctttaccggc cggtaagatg
     1501 ggttacttta tgggcgtgtt taattttttt attgtaattc cacaaattgt tgcctcgttt
     1561 tcattaggtc ccattttaaa atatgttttc aataatgaag cacaatacat gttattgtta
     1621 ggtggccttt cgatgattat tgccgctgtt atttgtacac gtgtgcaaga tagtgatgat
     1681 aaaaagttag gcgtataaat tagtttgttg gcatacctgt ggttgctgca gcttgatttt
     1741 taaaattaat ggttaagtct aaaaatcttt ttatatcggc atcatttggc gataacgata
     1801 gtgcgtttga aaaggcgtca atagatttat cataatcttt tatactacca taactgctac
     1861 ctaaattttt ccatgcatca acgtaattgg ggctcagttc aatggttttt aaataatact
     1921 taattgcatt attgtaatcg ccttttccaa aatatgccga ccctaagtta ttatatgcat
     1981 cagcataatc aggcattaaa acaatcgctt tttgaagaac ggtaattgca ctgtcatact
     2041 gttgcacttt aaagtacata ttgcctaagt tgttatatcc caaagctaag tttggcatac
     2101 gttgcactat ggttttataa taatacatgg cctgtttcat gtcgtttcgc tgcacataca
     2161 aatcacccaa gccaaaatag gcttcaaaat aatcaggatg tatttcaata cattttttta
     2221 attgcgtttc ggcaatgctg tagtattcaa cggttttttc ggggcctact tttttttgtt
     2281 tcacatcctg tataatgtga ttgtcatata aaaagcgcaa acgtgagctg gtaggtttgt
     2341 gttca
//
LOCUS       DAOMAA010000271         2341 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_41882_length_2341_cov_3.738845, whole genome shotgun sequence.
ACCESSION   DAOMAA010000271 DAOMAA010000000
VERSION     DAOMAA010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41882_length_2341_cov_3.738845"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="PLO59_04495"
     CDS             <1..780
                     /locus_tag="PLO59_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00395.1"
                     /translation="SLIETNLPAWAGQVSNWQKVELYDLNAMAGKQSVQFRFVFNSDM
                     VISNIGSFIDNFEIDEPKYNSVANQTLDLVTNLIFSYSAKKIKTEIKSVGYNPLYSCK
                     LSVIVDNTVLVTDTVYFNPPILYGKVYWVTSMFNLTQGNHTICTHTFFPNGVSDSIAY
                     DDIKCKTTFVYPMVNNIPSHLNIDSIANGFNTQRSPSNQTTDLPNTDKTIKVHPNPVS
                     QFLYVNFTGKVPDVLQIFTTTGLLVKQENLNTQNHNFLSAP"
     gene            1062..>2341
                     /locus_tag="PLO59_04500"
     CDS             1062..>2341
                     /locus_tag="PLO59_04500"
                     /inference="COORDINATES: protein motif:HMM:NF017368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="immune inhibitor A"
                     /protein_id="HQV00396.1"
                     /translation="MKTINILFSAIIFFTIHHVHAQINIPHCSNFNGANLWNVTTVSG
                     SAWQLGLPQYGATNSTHSPPSCFDIELNAAYLPNTVAYLQSPAFNLLSTPNSRMSFWL
                     NYNSELNWDGARLEYTIDNGVTWLVLGSMNDVLASNWYNSNAILSSNLPAWHGGSGGW
                     KKSTYNLNSFNNQPNISFRFVFTSDGAICTDGFSVDDFCITQAPDVDLRMDSVYIPAI
                     VSANAVVPVNAKVINMGLLPISNFNVYYNQNGTVQGPFVYNGTLQPGGSAIVNCGNLT
                     TIAGANNIKIYTAATNDADLSNDTLIRQMFVVGSSSLGCDDFENQSSWVAMPATGANS
                     WMLGMPNYSLTTGTHSGNNAWDVNLTTGIGAGTATLQSPTYTLSGNQAHQISFWINHA
                     LSTGYNGTTLLYSLNGSPYQPLGIVNDPNATNWF"
BASE COUNT          751 a          447 c          427 g          716 t
ORIGIN      
        1 agcttaattg aaaccaattt gcctgcctgg gccggccaag tatccaactg gcaaaaggta
       61 gagttgtatg atttaaatgc aatggcaggc aaacaatctg tacagttcag atttgtgttt
      121 aactcagata tggtaatttc taatataggc tcttttattg ataattttga aattgatgag
      181 cctaaataca attcagtagc caaccaaacg cttgatttgg ttaccaacct catatttagc
      241 tattcggcta aaaaaattaa aaccgaaata aaaagtgtag ggtacaatcc attgtacagt
      301 tgtaagctta gtgttatagt tgataacacc gtattagtta ccgatacggt ttattttaac
      361 ccacctatat tatatggaaa agtttattgg gttaccagta tgtttaatct tacccagggt
      421 aatcatacca tctgcacaca tacattcttt ccaaatggtg ttagcgattc tatagcttac
      481 gatgatataa aatgtaaaac caccttcgta tatcctatgg taaataatat accgtcacat
      541 cttaatattg atagtattgc caatggtttt aatacacaac gttcgcctag taatcaaaca
      601 acggatttgc ctaacactga taaaactata aaggtgcatc ccaatccggt tagccagttt
      661 ttatacgtta actttactgg caaagtgccc gatgtattac aaatttttac aaccacaggc
      721 ctgttggtaa aacaagaaaa cctgaacaca caaaaccata attttttatc ggcaccctag
      781 aaaatggggt gtattatata aaattaaaat cggcacttac aggacaaaaa tttgttgtta
      841 tgcattaaac agcatcatat aatcaaacta aataacgcag tattggtttt tattaacggg
      901 caagtttttt tagtgtttgc aacacaaaag ttagtgcacc atcagtaaaa ttaaatattg
      961 cccttgctca gttatgtcaa acccaattac ataaaactta tttaaagtaa aataagttac
     1021 tttaacaagc acattttgcg aattgtaatt tataatccac catgaaaacc atcaacatat
     1081 tatttagcgc tataattttt tttactattc atcatgtaca tgcgcaaata aacatacccc
     1141 attgcagcaa ctttaatgga gcaaaccttt ggaatgtaac taccgttagt ggttcggcat
     1201 ggcaactggg tttgccgcaa tatggtgcta ccaacagcac acactcgcca ccaagttgct
     1261 tcgatattga gttaaatgca gcatacttgc caaatacagt tgcttacctg caatcgcctg
     1321 catttaattt attatcgaca cctaatagcc gcatgtcgtt ttggctaaat tataatagcg
     1381 aattaaattg ggacggtgcc cgcctggaat ataccattga caatggcgta acctggcttg
     1441 tgttgggcag tatgaatgat gtattggcaa gcaactggta taattcaaat gcaatactat
     1501 catcaaattt gccggcatgg catggcggtt cgggcggctg gaaaaaaagc acgtacaact
     1561 taaactcatt taataaccaa cccaatattt ctttcaggtt tgtgtttact tcagatggtg
     1621 caatttgtac cgatggcttt tcagttgacg atttttgtat aacacaggcg cccgatgtgg
     1681 atttacgtat ggattcagtg tacatacctg ccatagtttc ggctaatgct gtggtgccgg
     1741 ttaatgcaaa agtcattaac atgggtttat taccaataag taactttaat gtttattata
     1801 atcaaaacgg aaccgtacaa ggcccgtttg tttataatgg cacattgcaa ccgggtggct
     1861 cagccattgt aaattgtggc aatttaacaa ccattgcagg agccaacaac attaaaatat
     1921 atacagcggc aaccaacgat gccgatttat ctaacgacac ccttatacgt caaatgtttg
     1981 ttgtgggtag cagctctttg ggatgtgatg attttgaaaa tcaaagcagt tgggttgcaa
     2041 tgccggcaac aggtgctaat agttggatgc tgggtatgcc aaactacagt ttaactaccg
     2101 gaacacattc gggtaataat gcatgggatg ttaatttaac cacaggtata ggagcgggta
     2161 cagcaacttt acaatcgcct acatatactt taagtggtaa tcaggcacat caaatttcgt
     2221 tttggataaa ccatgctttg tcaaccgggt ataacggcac cacactttta tattcattaa
     2281 acggtagccc ctatcaaccg ttaggtattg ttaacgaccc caatgctaca aattggttta
     2341 a
//
LOCUS       DAOMAA010000272         2339 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_41963_length_2339_cov_3.123468, whole genome shotgun sequence.
ACCESSION   DAOMAA010000272 DAOMAA010000000
VERSION     DAOMAA010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41963_length_2339_cov_3.123468"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="PLO59_04505"
     CDS             <1..801
                     /locus_tag="PLO59_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00397.1"
                     /translation="LVNSGTLNVGEANTSSIYRSGNDLAILPYAPISATPGNLFLCSS
                     SGLFKSGNVGIGTTSPTLAKLTVNGAIGNTVSLFRRSTNSAGISIVADWPGLYFNSYY
                     DGGTRVMTTGYAGLVNFNPTSGYLTFNVSPTSGANAGAIVTTPEAMFVHNDGSVSIGT
                     AVNAYKLNVCGTVRATEVRVSTGWCDYVFADDYNLRPLSEVESFIKENKHLPDVTKGS
                     IIESEGLEVGKTSAEMIRKIEELTLYIIQQQKRMDAMQTQIENLEAKK"
     gene            804..>2339
                     /locus_tag="PLO59_04510"
     CDS             804..>2339
                     /locus_tag="PLO59_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00398.1"
                     /translation="MKKLIVLCCLISASQAIAQEIINGTPGVISAPSGYMLSIKEAEA
                     SPLNIKYKAEPRLVEQFYPKKLRLDADAQKALHEAELQKTIEQFTVVHDSYKTAPTYL
                     PTQPNVSAPPENMVAQKITSQTPGRTGLGNQPGNLNPSDAQIAAGPNHIVVMANDDAR
                     ILNKQTGAIISEVTLGAFFGMSGFVFDPRIMYDPFGQRFVALALGKSGPNGCSAGNIN
                     SNFRLLVSNNNDPTQGWYWYDLDVDANNTSWMDFAMIGFNKDWICVAGNILCGNNVNG
                     IYVFNKSQVYAAASTSYYTFPQLWLAAPAFTYDNTLDKLYLTKTGNPNSGGLGYLSTY
                     YINSSAQLVTSTSYATSPWQAFASDSSRCPKQLGGQTFTFVNTFGASMLSNSVYRNGY
                     LWCTHPVFLPATGLTTRTSTLFWAVNPGNNTVYQTGFIDDVNGNTSTYHPSIAVNAAN
                     DAVVTYSIFNASYYQSAAYNSRNSGDPLGTLPNGVFYHSGTNTDADGRGGDYSQCAVD
                     PLDD"
BASE COUNT          701 a          515 c          487 g          636 t
ORIGIN      
        1 ttggtaaact cgggcacttt aaatgtgggc gaggcaaaca caagttctat ctaccgtagt
       61 ggcaacgatt tggctatatt gccttatgca cccatatcag ctacaccggg taacttattt
      121 ttgtgcagct cgtcaggctt atttaaatcg ggtaatgtag gtattggtac tacatcgcct
      181 acattagcca aattaaccgt aaacggtgct ataggtaaca cggtgagttt gtttcgcaga
      241 agtacaaaca gtgccggtat atccattgta gccgactggc ccggcttgta ctttaattca
      301 tattacgatg gcggaaccag ggttatgact accggctatg caggtttggt aaactttaat
      361 cctacatcag gctatttaac ttttaatgta agccccacat cgggtgccaa tgccggtgca
      421 atagttacta cccccgaagc catgtttgtg cacaacgatg gcagtgtatc tattggtaca
      481 gcagtaaatg cgtacaaatt aaatgtttgc ggtactgtgc gtgcaaccga agtacgcgta
      541 tcaacgggct ggtgcgatta tgtttttgct gatgattata acctacgccc ccttagcgaa
      601 gtagaatcat ttattaaaga gaacaaacat ttacccgatg taaccaaagg ctccatcatt
      661 gaaagcgaag gtttggaagt aggtaaaact tcggctgaga tgatcagaaa aattgaagaa
      721 cttaccttat atatcataca gcaacaaaag cgtatggatg ccatgcaaac acaaattgaa
      781 aacttagagg caaaaaaata agtatgaaaa aactcattgt actctgttgc ctcataagtg
      841 ccagccaggc aatagcgcaa gaaataataa atggcacacc gggagttatt tcggcaccga
      901 gtggatacat gttatctatt aaggaagccg aagcatcgcc actaaatatt aagtataaag
      961 cagaaccacg tttggttgaa cagttttatc ctaaaaaatt acgtttagat gccgatgctc
     1021 aaaaagcatt acatgaagcc gaactacaaa aaacgattga acagtttacc gtagtgcacg
     1081 attcatataa aacagcaccc acttatttac ccacacaacc caatgtaagc gcacctcccg
     1141 aaaatatggt tgcacaaaaa attacctcgc aaactcccgg ccgcacgggc ttgggtaacc
     1201 aaccgggtaa tttaaatcca agcgatgcac aaattgctgc aggccctaat cacattgtgg
     1261 tgatggctaa tgacgatgca cgtatattaa acaaacaaac tggtgccata attagcgaag
     1321 ttacattagg ggcttttttc ggcatgagcg gttttgtgtt tgacccacgc attatgtacg
     1381 acccttttgg acaacgtttt gtagccttgg cattaggtaa aagtggcccc aatggttgta
     1441 gtgccggcaa catcaactcc aatttcagat tattggtaag caacaacaac gaccctacac
     1501 aaggttggta ttggtacgat ttagatgtgg atgccaataa cacatcctgg atggattttg
     1561 ccatgatagg ttttaataaa gattggatat gtgttgcggg caacatactt tgcggcaaca
     1621 acgtaaacgg tatttatgtt tttaataaat cgcaagttta tgctgctgca tcaacatctt
     1681 attatacatt cccacagctt tggttggctg cacccgcctt tacatacgat aataccctcg
     1741 acaaattata ccttaccaaa actggcaacc ccaatagtgg aggcttaggg tacttgtcaa
     1801 cctattacat aaacagctcg gcacaattag ttacatcaac cagctatgcc acatcgccct
     1861 ggcaagcctt tgccagcgat agtagccgtt gccccaaaca attaggcggg caaactttta
     1921 cattcgtaaa tacatttggt gccagcatgt tatcaaacag cgtatatcgt aatggttatc
     1981 tgtggtgtac acatcctgta tttttaccgg caaccggttt aaccacccgt acttctacac
     2041 ttttttgggc tgttaacccg ggtaataaca ccgtgtatca aaccggtttt attgacgatg
     2101 tcaatggcaa tacctctacc taccacccca gtattgcagt taatgcagct aacgatgcag
     2161 tggttactta cagtattttt aatgccagtt actatcaaag tgcagcctat aactcgcgta
     2221 acagtggcga cccattgggc acattaccta atggtgtgtt ttatcatagt ggcacaaata
     2281 ccgatgccga tggccgcgga ggcgattata gccaatgcgc tgttgacccg cttgacgat
//
LOCUS       DAOMAA010000273         2333 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_42168_length_2333_cov_3.543459, whole genome shotgun sequence.
ACCESSION   DAOMAA010000273 DAOMAA010000000
VERSION     DAOMAA010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42168_length_2333_cov_3.543459"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            14..>2333
                     /locus_tag="PLO59_04515"
     CDS             14..>2333
                     /locus_tag="PLO59_04515"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HQV00399.1"
                     /translation="MLLKINLTITHGLSPQGGYAFSELGLLFFIMGNYKYAYPAATLS
                     KRLAEKFKKESPRNLSRSGHLFTNYNTPWVKHISETAKLNPEYYQVSIDCGELIFAGY
                     TSFHPFFNSYFQGNESLQTLLLRIPDALEFTKKIKHDLAHDSLQALQLVMSNLAGATN
                     AETSFDLPNKSGAALLQYCKDAKDAYGSTIFHLYKAQAYYLYGNLAEASYCLQVVAGL
                     PGVIAGNVPSHATFNMLYALTLLGMIESAAESETETYWNNINTFKEQLQNWSTHNASN
                     FEHKYFLVEAEYMRVKGDILKAIEFYGKAIESAELYDFDREVALAHQKAGAFWISMSN
                     KIYAQPHLERARYIYDKLGYQRLAKAIDNKYPFVNKNTVSTKRTDIFSTLYSSINTAD
                     LAGFDMKSVMKASHALSEEIVYEKLLSKILRIVIENAGGQRAVLVLNYNNKWRIEADF
                     NVEDADIHTMESADIDMATNIPVSVINYVIRTRTQVLSFVNSSIKIFERDEYFKSYNP
                     ASYMCLPLSHKGATNGIIYVEHRSNADAFSSERLGILELLTSQMSVSIENATLYKKQF
                     ELNQAYQRFVPHDFINAIGRQSILEVKLGDHIKRDMIVMFCDIRAYSAMSEGMTTEEN
                     FAFINAYLQRVGPVIQSHNGFINHYFGDGFIALFQGEPESALQAAVAILKEIESYNKE
                     RVSNQQQPIAVGIGLHKGPIMMGIIGDNQRHDANVLSDAVNIASRLEGLTKIFGANVV
                     LSENLYQALGNLELFQFRYLGRIKVKGKQEVMS"
BASE COUNT          744 a          430 c          478 g          681 t
ORIGIN      
        1 gtggattttg caagtgttat taaaaataaa cttaaccatt acgcatgggt tatcaccgca
       61 aggtggctat gcattttctg aattgggcct gttgtttttt ataatgggca attacaaata
      121 tgcttacccg gctgccacac ttagtaaacg tttggccgaa aaatttaaaa aggaatcgcc
      181 acgtaacctc agccgttcag gccatttatt tacaaattat aatacgcctt gggttaaaca
      241 catttcagaa actgcaaaac taaaccccga gtattatcag gttagtattg actgtggcga
      301 acttattttt gcaggctata ccagttttca tccatttttt aactcctatt ttcaaggtaa
      361 cgaatcgctg caaacgttgc tcttgcgcat acccgatgcg ttggagttta ctaaaaaaat
      421 aaagcacgat ttagcacacg actcgttgca agcattgcaa ttagtaatgt caaatctagc
      481 aggtgcaacc aatgccgaaa ccagtttcga tttacccaat aaatcaggag ctgccttgtt
      541 gcaatattgc aaagatgcca aagatgctta cggctctacc atatttcatt tatacaaagc
      601 gcaagcgtat tatttatacg gcaacttagc cgaagcttca tactgcttgc aggttgttgc
      661 agggttgccc ggtgtaatag ctggcaatgt accttcgcat gctacattta atatgcttta
      721 cgcgcttact cttttaggta tgattgaaag cgctgccgaa agtgaaactg aaacctactg
      781 gaacaacatt aatactttta aagaacagct acaaaactgg agtacacata atgcttctaa
      841 ttttgagcac aaatactttt tagttgaagc agaatacatg cgcgtaaaag gcgatatttt
      901 aaaagcaatt gaattttatg gtaaggcaat tgaatcggcc gagttatatg atttcgacag
      961 ggaggtagca ttagcgcatc aaaaggccgg agcgttttgg attagcatga gtaataaaat
     1021 ttatgcacaa ccccatttag aacgtgcccg atatatttat gataaattag gctatcagcg
     1081 tttagcgaaa gctattgata ataaatatcc gttcgtaaat aaaaacacgg tatcaacaaa
     1141 acgtaccgat atattcagta cgctttacag cagtatcaac acagccgacc tggcaggctt
     1201 tgatatgaaa agtgttatga aagcttcgca tgcattaagc gaagaaattg tgtatgagaa
     1261 actgctttca aaaattttgc gtatcgtaat tgaaaatgcc ggtggacaaa gggctgtatt
     1321 ggttttaaat tacaataata aatggcgcat cgaagccgat tttaacgttg aagatgcaga
     1381 catacacacc atggaatcgg ctgacattga tatggctacc aatatacccg tatcggtaat
     1441 aaattatgta attcgaacac gtacgcaagt gctttcattt gtaaacagtt cgattaaaat
     1501 attcgaacgt gacgaatatt ttaaatctta taatccggca agttatatgt gcctgccctt
     1561 atcgcacaaa ggcgccacta acggaattat ttatgttgaa catcgttcaa atgccgatgc
     1621 gttttcaagc gagcgattgg gtattttaga attgcttacc agccaaatgt ccgtctcaat
     1681 cgaaaatgca acactatata aaaagcaatt tgaattaaat caggcttacc aacgttttgt
     1741 tccccacgat tttataaatg ctattggccg ccaaagcatt ttagaggtaa aactgggcga
     1801 tcatattaaa cgcgatatga ttgtgatgtt ttgcgatata cgtgcatact cggcaatgag
     1861 cgaaggcatg actactgaag aaaattttgc atttatcaat gcctacctgc aacgtgttgg
     1921 gccggtaata caaagccaca acggttttat aaatcattat tttggagatg gttttattgc
     1981 actctttcag ggcgaacccg aaagcgcatt acaagccgca gttgctattt taaaggaaat
     2041 tgaatcgtac aataaggagc gtgttagcaa ccagcaacag cctattgctg tgggcattgg
     2101 tttgcacaaa ggcccaataa tgatgggtat tattggtgat aatcaaaggc acgatgccaa
     2161 tgtattaagc gatgctgtaa acattgcttc gaggttagaa gggctaacaa aaatatttgg
     2221 agccaatgtg gtattaagcg aaaacctata ccaggctttg gggaatttag aattgtttca
     2281 gttccgttat ttaggtcgta ttaaagtaaa aggtaagcag gaggtaatgt cgg
//
LOCUS       DAOMAA010000274         2320 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_42622_length_2320_cov_3.258720, whole genome shotgun sequence.
ACCESSION   DAOMAA010000274 DAOMAA010000000
VERSION     DAOMAA010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42622_length_2320_cov_3.258720"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..1718)
                     /gene="nadB"
                     /locus_tag="PLO59_04520"
     CDS             complement(141..1718)
                     /gene="nadB"
                     /locus_tag="PLO59_04520"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008507731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HQV00400.1"
                     /translation="MQTEVDFLVIGSGIAGLSYALKVAQHGSVMLVTKSNADESNTKY
                     AQGGIAAVFDAPDTYQKHVQDTLIAGDGLCKTEVVHTVVTEGSERVRELIAWGAAFDK
                     TKDGTYDLAREGGHSEHRILHYKDVTGLEIERALLEKINAHPQIKLYTHLFAVDIITE
                     HHLGQEINSQSENITCFGAYLLDTLTGKMQTVLARKTLMATGGAGHVYKTTTNPAIAT
                     GDGVAMVYRAKGAVNGMEFIQFHPTALYNPGQNPCFLISEAVRGFGAVLKTHNGHEFM
                     QMYDDRKSLAPRDIVARAIDNEMKKSGIDYVYLDATHLPMNDFKVHFPNIYAKCKSLG
                     IDVATQLIPVVPACHYMCGGITVDLNGCSTIKNLYAVGECASTGLHGANRLASNSLLE
                     AVVFSHRAAMHAVNEFKQIGFENRVPKWNAEGTSQPNELVLITQNLNELQSLMSNYVG
                     IVRTNQRLKRALDRLRIIYGETEALYGRTVVSQQLCELRNLITIAYLIVKQATARKVN
                     VGLHYNSDNAIATNDAK"
     gene            1906..>2320
                     /locus_tag="PLO59_04525"
     CDS             1906..>2320
                     /locus_tag="PLO59_04525"
                     /inference="COORDINATES: protein motif:HMM:NF020295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU N-terminal domain-containing protein"
                     /protein_id="HQV00401.1"
                     /translation="MSTDKPLQPIEIYMEQTPNPDALKFVTNILLLPQGSVNYNHKEA
                     ALYCPLAYQLFDFSGVTNVFITTHFVTVTKNTEIDWWEIQNIVREFIRGFLLAGDAVF
                     TRNPLPDAPEQNAAIAAEIEPAPKTQGPLDEKIIAL"
BASE COUNT          669 a          489 c          462 g          700 t
ORIGIN      
        1 tggtgctaac atctacatta cgcaattgca aaaactgttt ataaacaggc actgaaacgg
       61 cttgcaaatt aaaaagctgc aatgctgttg attcaaaatc tgatgaatta tcgaaaatcg
      121 aatcagctaa aaacttcagt tcattttgca tcattagttg ctattgcatt gtcggaatta
      181 tagtgtaaac ctacatttac tttgcgtgct gtagcctgtt ttacaattag gtaagcaatg
      241 gtaataaggt tacgtaattc gcaaagttgt tgcgatacaa cggtgcgccc gtacaaagct
      301 tcggtttcgc cataaataat gcgcaagcgg tctaaagcac gttttagcct ttgattggtg
      361 cgtacaatgc ccacataatt actcataagg ctttgcaatt catttaggtt ctgtgtaatt
      421 aaaaccagtt cgttgggttg cgaggtgcct tcggcattcc acttgggtac gcggttttcg
      481 aaaccaattt gtttaaattc atttacggca tgcatggctg cacggtgcga aaacactacc
      541 gcttcgagca aagagtttga ggcaagtcgg ttggcaccat gcaaaccggt tgatgcacac
      601 tcgcccacgg catataaatt tttaatggtg ctgcagccgt ttaaatcaac agtaataccg
      661 ccacacatat aatggcaggc aggcacaacg ggtattaact gcgttgccac atcaataccc
      721 aaacttttac acttagcata tatgttcggg aaatgcactt taaagtcgtt catgggcaaa
      781 tgtgtggcat ctaaatacac atagtcaata ccgctttttt tcatttcatt atcaatggca
      841 cgggccacaa tatcacgagg tgccaacgat tttctatcat catacatttg cataaactca
      901 tggccattat gcgttttaag cactgcacca aaaccgcgca cggcttcgct aattaaaaag
      961 catgggtttt ggcccgggtt gtataatgct gtaggatgaa attgtataaa ctccattcca
     1021 tttaccgcgc ctttggcacg atacaccata gccacaccat cgcctgtggc aatggcaggg
     1081 ttggttgttg ttttataaac atggccggca ccacctgtag ccattaatgt tttgcgtgcc
     1141 agtacggttt gcattttacc ggttaaggta tcgagcaaat aggcgccaaa acaggtaatg
     1201 ttttccgatt ggctgtttat ttcctgacct aaatggtgct cggtaataat atcaacagca
     1261 aacaaatggg tgtacaattt tatttgcggg tgtgcattta ttttttctaa taaggcgcgt
     1321 tcaatttcta atccggtaac atctttataa tgcaaaatac ggtgctcgct atggccacct
     1381 tcgcgtgcca aatcgtaagt gccgtctttg gttttatcaa aggctgcccc ccacgctatc
     1441 agttcgcgta cacgttcgct gccttcggta actacggtat gcactacttc ggttttgcac
     1501 aggccatcgc ccgcaattaa agtatcctgt acatgtttct gataggtatc aggggcatca
     1561 aaaacggcag caataccacc ctgtgcatat ttggtattac tttcatcggc atttgatttg
     1621 gtaaccaaca ttacactgcc atgttgggca acttttaaag cataacttaa tccggctata
     1681 cccgacccta ttactaaaaa atcaacttcg gtttgcataa ctataacagg ttattgggcc
     1741 aaaagcccga ataatttatg gtgcaatagt aacttattat gaagcatggt ttaatttttt
     1801 caattttgcc ggcaatatgt tttaagccgt gcctggaggt gtatttgttt accggctaaa
     1861 attttattaa aagatgtatt taaattattc aataaataaa aaatcatgtc aaccgataag
     1921 ccgctacagc ccattgaaat ttatatggag caaacaccca accccgatgc actaaagttt
     1981 gttaccaata ttttattgtt accacaaggc agtgtaaatt ataatcataa agaagctgca
     2041 ttgtattgcc cattggccta tcagttgttt gattttagtg gcgtaaccaa tgtatttatt
     2101 acaacgcatt ttgttactgt tactaaaaat acagaaatag actggtggga gatacaaaac
     2161 attgtacgcg aatttatacg cggtttttta ttggccggtg atgccgtatt tacacgtaat
     2221 ccgttgccgg atgcccccga acaaaatgcg gctattgctg ccgaaataga accggcacca
     2281 aaaacacaag gccctttaga cgaaaaaata attgccttgt
//
LOCUS       DAOMAA010000275         2319 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_42662_length_2319_cov_3.532244, whole genome shotgun sequence.
ACCESSION   DAOMAA010000275 DAOMAA010000000
VERSION     DAOMAA010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42662_length_2319_cov_3.532244"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="PLO59_04530"
     CDS             complement(<1..536)
                     /locus_tag="PLO59_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQV00402.1"
                     /translation="MSKETVLVLGSSGQIGTDLVHALRNIYGNDNVIAADLKAQPIAI
                     GQAGPFEFIDVLDAVKISETINKYKVTQVYLLAALLSATAEQKIKSAWRLNMEGLFNL
                     LEIAREQKHLKIFWPSSIAVFGPTTPRQNTPQYTVMEPSTVYGISKLAGERWCEYYHQ
                     KFAVDVRSIRYPGLISYKA"
     gene            complement(596..1339)
                     /locus_tag="PLO59_04535"
     CDS             complement(596..1339)
                     /locus_tag="PLO59_04535"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HQV00403.1"
                     /translation="MKDFFKLLLQKTLGFDNYLFVFSKFKINTLRFDKNEGDFLHFVK
                     LISKPCTVLDIGANIGIMAITFYKQLPQSHIIAFEPMPANLKALNRIIKSYRANQIVV
                     EPIALGNSNGTLEMVMPMRNNVKFQGLSHVVHESIPHDDGIIVKVPVQTLDTLPILQQ
                     LKLPVGAIKLDVENFEFFVLDGGKQLIKKYKPIIYAELWDNENRKQCFVLMQSLGYEI
                     QVLINHALVTFNADVHQKQNFFFIPISTV"
     gene            1489..>2319
                     /locus_tag="PLO59_04540"
     CDS             1489..>2319
                     /locus_tag="PLO59_04540"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HQV00404.1"
                     /translation="MKLVVNTRHITPQGYDGIGRFTFEIFKRLALQQPQHEFVFLFDR
                     PFDKKYITASNITGKVLFPPTKHAWLIKCWYQLSLTWFLKRNKADIFVSTDGLLSNYT
                     HTPQLAVIHDLNFIHVPDLKSSYTQFFKKFYAQSASKAARIATVSQFSKQDIAGNYHV
                     PLSKIDVVYNAAADFYKPLPLHTQQEIRNQYTSGKPYFIFVGSLHQRKNIIRILQAFE
                     ALSSEQNQFHLVIAGNLRWASQDINKAYEAMQHKNKVVFTNRISDTQLHQLLASAKAL
                     V"
BASE COUNT          743 a          455 c          404 g          717 t
ORIGIN      
        1 gccttatagc ttattaatcc cggataacga atactgcgca catccactgc aaatttttga
       61 tgataatatt cgcaccaacg ttcgcccgct aatttgctga tgccataaac tgtgctgggt
      121 tccataacag tgtattgggg tgtgttttga cgtggcgtag tggggccaaa aactgctatc
      181 gaactgggcc aaaatatttt caggtgtttt tgttcgcgtg ctatttccaa caaattaaaa
      241 aggccttcca tatttaaccg ccatgccgat tttatttttt gttcggctgt ggccgataat
      301 aaagccgcta acaaatacac ttgtgttact ttgtatttgt tgatggtttc cgaaattttt
      361 accgcatcaa gcacatcaat aaattcaaac ggcccggcct gaccaatagc aataggctgt
      421 gcttttaaat cggcagcaat tacattatcg tttccataaa tattgcgcaa tgcatgtacc
      481 aagtcggtac ctatctgacc tgacgaccca agcactaaaa cagtttcttt gctcatgttt
      541 attatattaa aaaaggagtt tttaaatgcc cttacatttt tttcaaataa aataattaaa
      601 cggtacttat aggtataaaa aagaaatttt gcttttgatg cacatcagca ttaaacgtaa
      661 ctaaggcgtg gtttattaaa acctgtattt catagcctaa cgattgcatt aaaacaaagc
      721 actgtttgcg gttttcatta tcccacaact cggcatatat aattggcttg tattttttta
      781 taagctgttt gccaccatct aaaacaaaaa attcaaaatt ttcgacatct aatttaatag
      841 caccaacagg taattttaat tgctgcagaa tgggtaaggt atccaaagtt tgaacaggta
      901 cttttacaat aatgccatca tcatgtggta tgctctcatg tactacatga ctcagacctt
      961 gaaattttac gttattgcgc atgggcataa ccatttctaa cgtgccgttt gaattaccta
     1021 atgcaatggg ctcaacaaca atttggtttg cacgatagct ttttataatc cggtttagtg
     1081 cttttaagtt tgccggcata ggttcgaaag caataatgtg cgattgtggt aattgcttgt
     1141 aaaacgttat ggccattatg ccaatgttag cgccaatatc taatacagtg cacggtttcg
     1201 aaatcagttt tacaaaatgt aaaaagtcgc cttcgttttt atcaaatcgt aaagtgttta
     1261 ttttaaactt cgaaaacaca aataagtaat tatcgaagcc taacgttttt tgcagcaaca
     1321 gtttaaaaaa atctttcata tcagttaaaa aacaaaaagc tctctacaat gctaatttgg
     1381 cctgagcacc tttttaagcc ggtttcgggc aataataaca aaccaaagct atagtcttat
     1441 aatactacat ttgcgcgcaa agaaaaacaa aaaaccaaaa gccattcatt gaaattagta
     1501 gttaataccc ggcacattac accgcaagga tatgatggca ttgggcgctt tactttcgaa
     1561 atatttaagc ggttagccct tcaacagccg caacatgagt ttgtattctt gttcgacagg
     1621 cctttcgata aaaaatacat tacggcatca aatattacag gcaaggtttt atttccacct
     1681 actaaacatg cttggttaat taagtgctgg tatcaactat cgctaacttg gtttttgaaa
     1741 aggaacaagg ccgatatttt tgtgagtacc gatggtttgc taagtaatta tacacataca
     1801 ccacaactgg cagtaataca tgatttaaat tttattcatg tgcccgacct taaaagcagc
     1861 tatacccaat tctttaaaaa gttttatgca caaagtgcaa gcaaagctgc gcgtatagct
     1921 actgttagcc aatttagtaa acaggatata gccggcaatt atcatgtgcc acttagtaaa
     1981 attgatgtag tgtataatgc cgcagccgat ttttacaaac cattgccatt gcatacacag
     2041 caagaaatca gaaatcaata tacaagcggc aaaccctatt ttatttttgt tggctcatta
     2101 catcaacgca aaaatataat acgcatacta caagcatttg aagcgttgag cagcgagcaa
     2161 aatcaattcc acctcgttat agccggtaac ctaaggtggg catcgcagga tattaataaa
     2221 gcctatgaag ccatgcaaca caaaaacaag gtggtgttta ccaaccgcat ttctgacaca
     2281 caattgcatc aattgctggc atcggctaaa gctttggtt
//
LOCUS       DAOMAA010000276         2314 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_42858_length_2314_cov_2.780434, whole genome shotgun sequence.
ACCESSION   DAOMAA010000276 DAOMAA010000000
VERSION     DAOMAA010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42858_length_2314_cov_2.780434"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="PLO59_04545"
     CDS             complement(<1..394)
                     /locus_tag="PLO59_04545"
                     /inference="COORDINATES: protein motif:HMM:NF027718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HQV00405.1"
                     /translation="MKKQIFALSALVMLGSTLFIGCSEDDTTAPSITILGNNPTVSLK
                     DVTYTDAGATANDDKDGDITASIVTTNGVNKNVAGSYVVSYSVADKAGNTAAEDRDVY
                     VVDLNGTYNVVDVVGGSSSNYTDVVTVTG"
     gene            551..946
                     /locus_tag="PLO59_04550"
     CDS             551..946
                     /locus_tag="PLO59_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00406.1"
                     /translation="MKKVFALLLTAGVCAIVACGPSAEEKAKAEQATKDSLAAVEMQR
                     VADSTAAAMAAQEAAMKDSLDKAALDGQMQAMMDSMNAIKGAAEKATAEAKAAAAKAA
                     AAKAAADKAKKQQEEQKINTNVKAGQGKG"
     gene            complement(1019..1558)
                     /locus_tag="PLO59_04555"
     CDS             complement(1019..1558)
                     /locus_tag="PLO59_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00407.1"
                     /translation="MKKFFLTALSISVGIVHAQSNVVVLEKYPNQSARQTISYAADSS
                     YTVLTDYYKSGVKQNVDTLINDHVNGWSTQYNKDGTIFIQQLYDFDKPIVQKFYLYYN
                     DGKIKTAWESDTLGRIEDNREVRPANGYWKEYYHNGNLKMCGRKNQNSNNDGEWIYYD
                     ENGIERKRMFYIDGKSLDK"
     gene            complement(1572..2117)
                     /locus_tag="PLO59_04560"
     CDS             complement(1572..2117)
                     /locus_tag="PLO59_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015362208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HQV00408.1"
                     /translation="MNNEKTPNDALQRKTIDEFKTAKKTPVIIILDNVRSAINVGSIF
                     RTADAFLIEAVYLCGITAQPPHKEILKSALGATDTVKWMWFENVADAIMHAQQNGFLV
                     YAVEQTQQSILLNNFKHVENRPIAVIFGHEVTGVSQEAINLCQACIEVPQQGHKHSLN
                     ISVCCGIVLWHLCHQVFPNKS"
BASE COUNT          724 a          442 c          386 g          762 t
ORIGIN      
        1 caccggtaac ggttaccaca tcggtataat ttgatgatga accacctaca acatctacta
       61 cgttataggt accattaagg tcaactacat aaacgtcacg atcttcggct gctgtattac
      121 ctgctttatc agctactgaa taacttacca cataagagcc tgctacattt ttatttacac
      181 cattggttgt aacaatgctg gctgttatat cgccatcttt atcgtcattt gcagtagcac
      241 ctgcatcggt ataagtaaca tcctttaaac ttacggtagg attattacct aaaattgtaa
      301 ttgatggtgc tgtagtatcg tcttcagaac aaccaataaa taaagttgag cctaacatta
      361 ccaaagcaga aagtgcaaaa atttgttttt tcatttttta atatttttta gatttaaatt
      421 tcggggtgca aatgtaatac catttttaag ttgtcaaatt aagtacttca tcaaatattt
      481 aaatttcaat ttttgaattt tagcttacat tcgcggccga atttttaaaa atctcaaaaa
      541 caacaaaaaa atgaaaaaag tatttgcctt attacttact gccggtgttt gtgcaattgt
      601 tgcctgcggc ccaagcgctg aagaaaaagc taaagctgaa caagctacta aagactcgtt
      661 agccgctgtt gaaatgcaac gtgttgctga ctcgactgct gctgcaatgg ctgcacaaga
      721 agctgcaatg aaagattcgt tggataaagc cgcattagac ggacaaatgc aagccatgat
      781 ggacagcatg aatgcaataa aaggcgctgc cgaaaaagca actgccgaag ctaaagccgc
      841 tgctgctaag gctgctgctg ctaaagccgc tgccgataaa gctaaaaaac agcaagaaga
      901 acaaaaaatt aacaccaacg ttaaagctgg tcaaggcaaa ggttaataac cttttgattt
      961 acttgttaac acaaaggccc cggtttatag ctggggcttt tgctttatat aaagttattt
     1021 atttatctaa agatttgcca tcaatataaa acatacgttt gcgctcaata ccattctcat
     1081 cataataaat ccactcacca tcattgttac tgttttgatt tttgcgtccg cacatcttta
     1141 agttgccgtt atggtagtat tccttccaat aaccattagc gggtctcact tctctgttat
     1201 cttctattct gcctaatgta tccgactccc acgctgtttt aattttacca tcgttatagt
     1261 agagataaaa cttttgaact atgggtttat caaagtcata aagttgttgg ataaaaatgg
     1321 taccatcctt attgtattgt gtactccaac cattaacatg gtcatttatc aaagtatcta
     1381 cattttgttt tacgccactt ttatagtagt cggttaaaac ggtgtaactg ctatcagcag
     1441 cataactgat agtttgcctt gcactttggt ttggatactt ttccaataca acaacatttg
     1501 attgtgcatg tactatgcca actgaaattg ataatgcggt taaaaaaaac tttttcatgt
     1561 gtgtgtgtta tttatgattt atttggaaat acctgatggc acagatgcca caaaacaata
     1621 ccacagcaaa cagaaatatt tagtgaatgt ttgtgtcctt gctgtggaac ttcaatacaa
     1681 gcctggcata agttaattgc ttcttgtgat acacctgtta cttcgtggcc aaaaataact
     1741 gcaattggtc ggttttcgac atgcttaaaa ttatttaata aaatactttg ttgtgtttgt
     1801 tcaactgcat ataccaaaaa gccattttgt tgtgcatgca ttatggcatc ggcaacattt
     1861 tcgaaccaca tccattttac cgtatcggta gcacctaaag cactttttaa aatttcctta
     1921 tgcggtggct gcgctgtaat accacataaa taaacagctt caattaaaaa tgcatcagcc
     1981 gtacgaaaaa tactgcctac attaattgca cttcttacat tatctaaaat aattattaca
     2041 ggtgtttttt ttgctgtttt aaattcgtca attgtctttc gttgtaatgc atcgtttggt
     2101 gttttttcgt tattcatagt tttgaacaaa ttgggaaaat aattgccgct aagttgcata
     2161 ttagcgcgat aaatcacagt gataaatggc tgctattctt atgtcatgac tatcttttat
     2221 acaaaatatt tttatcaata aaaaagccgc tgattttcat tatgacttca aaaaacacag
     2281 ccggcaaaaa tacatcgccc gaaacgcctt taat
//
LOCUS       DAOMAA010000277         2312 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_42928_length_2312_cov_3.097475, whole genome shotgun sequence.
ACCESSION   DAOMAA010000277 DAOMAA010000000
VERSION     DAOMAA010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42928_length_2312_cov_3.097475"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..622)
                     /locus_tag="PLO59_04565"
     CDS             complement(95..622)
                     /locus_tag="PLO59_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00409.1"
                     /translation="MIDKSLFDILFYLVSPCLVMATTYIMLQKFFEREQRMQLFELKK
                     NTQQHTLPLRLQAYERMVLYVERMMFNNLINRLNQSDFTVRQLQFSMINNLRDEYEHN
                     VAQQLYMSSEIWMLIKQTKEETIRIIISIAENIDAEAPSAQLAKGLFEYIINNDYIKH
                     QQTLDALKAEARQIF"
     gene            844..2034
                     /locus_tag="PLO59_04570"
     CDS             844..2034
                     /locus_tag="PLO59_04570"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HQV00410.1"
                     /translation="MKLSQRIQELSESQTLVMAKLSRELKEQGHDIISLSLGEPDFVT
                     PEHICNAAKLALDEGYTYYPPVAGFADLRQAIVTKFKTENQLDFKPNQIVVSAGAKQS
                     IANIIFSLINPGDEVIIPTPYWVSYSQMVTLAGGIPVFINANIETDFKVSAEQIEAAI
                     TPRSRLFIFSSPCNPSGSIYNKAELESLAQMFAKHTHIMILSDEIYEHINFAAKHESI
                     AQFDSVKDQTIIVNGVSKAYAMTGWRLGYMAAPVVVAQACEKIQGQFTSGTNSIAQKA
                     AIAALQTPISLLAEMKRSFLRRRNLIIANLKDIPGILVNEPQGAFYVFPDVSAFFGKK
                     HDGGTIQTANDLSMYLLSDAKVAVVTGEAFGNDNCIRLSYATSDHLLVDAADRIRKSL
                     AKLS"
     gene            2058..>2312
                     /locus_tag="PLO59_04575"
     CDS             2058..>2312
                     /locus_tag="PLO59_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002698616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HQV00411.1"
                     /translation="MSNKVEALFKNISRLNVLILGDVMIDAYMWGKVDRISPEAPVPV
                     VAVTKRESRLGGAANVAINIQALGATPYLCGIIGNDTAGQH"
BASE COUNT          748 a          449 c          457 g          658 t
ORIGIN      
        1 acatgggtgc tttggtacgt gccagcttac gcgaaatgat taaacgcaac agccagcatt
       61 ttaaaaaaat ggctgccaaa cgtgtaaaag attattaaaa aatctgacgg gcttcggctt
      121 ttaaggcatc aagagtttgc tgatgcttaa tgtaatcgtt attgataata tattcaaaca
      181 agcctttggc taattgtgcc gatggtgctt cggcatctat attttcggct atcgaaatta
      241 taatgcgtat ggtttcttct ttggtttgtt ttataagcat ccaaatctca ctactcatgt
      301 aaagttgctg ggcaacatta tgttcatact catcgcgcaa gttgttaatc atcgaaaact
      361 gcaattgcct aaccgtaaaa tcgctttgat taagccggtt tattagatta ttaaacatca
      421 tacgctcaac atacaaaacc atgcgctcat aagcttgcaa acgtaaaggt aaagtatgtt
      481 gctgtgtatt ctttttcagt tcgaaaagtt gcatgcgttg ttcgcgttca aaaaactttt
      541 gaagcataat gtaagtagtt gccataacca aacagggcga aaccaaataa aaaagtatat
      601 cgaacaaact tttatcaatc ataaaatttt tataaaaaag gtaaatgtgt tgcgctaaaa
      661 cgaagctgaa atcagataat tattatgggg caaaagtaaa tttatactgt tacgtatcag
      721 caacatcaac ccataaaaaa cctaacagtt ttaaaatgtg taaatgttta aataaccaat
      781 cagtaaaaaa cagcatgcta aaagtaattt ttagctttgc ggcccaaacc aatagagcaa
      841 tccatgaaat tatcgcaaag gatacaggaa ttatccgaaa gccaaacctt ggtaatggct
      901 aaactaagtc gcgaattaaa agaacaaggc cacgatatta ttagcctgag tttaggcgag
      961 cccgattttg taacacctga acatatttgc aatgcagcta aattagcttt agacgaagga
     1021 tacacctatt acccaccggt tgccggcttt gccgatttgc ggcaggccat agtaaccaag
     1081 tttaaaaccg aaaatcagct agattttaaa cccaatcaaa ttgtggtttc ggccggggcc
     1141 aagcaatcaa ttgccaatat catttttagt ttaataaacc cgggcgatga agtaattata
     1201 cctacaccat attgggttag ttactcgcaa atggtaacat tagcaggtgg catacccgtt
     1261 tttattaatg ccaatatcga aaccgacttt aaagtaagtg ccgagcaaat tgaagcagca
     1321 attacaccac gcagtcgttt atttattttc tcatcgccat gcaacccaag cggcagtatt
     1381 tataacaaag ccgaactcga aagtttagca caaatgtttg ccaagcatac acacatcatg
     1441 attttaagtg atgagattta tgagcacata aattttgctg caaaacatga gagtattgcc
     1501 caatttgatt ctgttaaaga tcaaaccatt atcgtaaatg gtgtttcgaa agcgtatgcc
     1561 atgaccggat ggcgcttggg atatatggca gcgcctgttg ttgttgccca ggcttgcgaa
     1621 aaaattcaag gccagtttac atcaggcact aactccattg cacaaaaggc cgctattgct
     1681 gctttacaaa cacccataag tttattggcc gaaatgaagc gctccttttt acgaagacgc
     1741 aacttaatta ttgccaacct taaagatata cctggcatac tggttaacga accacagggg
     1801 gcattttatg tgtttccgga tgtgagtgcc ttttttggta aaaaacatga tggtggcacc
     1861 atacaaaccg ctaacgattt atccatgtat ttactgagcg atgccaaagt tgcagttgtt
     1921 acaggcgaag cttttggcaa cgacaattgc atcagattat cgtatgcaac aagcgaccac
     1981 ttattggtag atgctgccga ccgtattcgc aaatcacttg caaaacttag ttgatttaac
     2041 agcaaaccgt taatctgatg tcaaacaagg ttgaagcact ttttaaaaat ataagccggt
     2101 taaatgtttt aattctgggc gatgtgatga ttgatgccta catgtggggc aaggtagatc
     2161 ggatatcgcc cgaagctccg gtaccggtgg tagctgtaac caaacgcgaa tcgcgtttag
     2221 ggggtgcagc caatgttgca attaatatac aggcattagg cgccacacca tacttatgtg
     2281 gcataattgg taatgatacg gctggtcaac at
//
LOCUS       DAOMAA010000278         2312 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_42939_length_2312_cov_2.462118, whole genome shotgun sequence.
ACCESSION   DAOMAA010000278 DAOMAA010000000
VERSION     DAOMAA010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42939_length_2312_cov_2.462118"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="PLO59_04580"
     CDS             <1..410
                     /locus_tag="PLO59_04580"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013452259.1"
                     /note="catalyzes the formation of
                     4-imidazolone-5-propanoate from urocanate during histidine
                     metabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HQV00412.1"
                     /translation="LKFNELVRKKKVKAPIVIGRDHLDCGSVASPNRETEAMKDGSDA
                     IADWPVLNALINTAGGASWVSLHHGGGVGIGYSIHAGMVIVADGTDAAATRIKRVLHN
                     DPAMGVLRHMDAGYDIATDTARKHRLDIRERLK"
     gene            528..2213
                     /locus_tag="PLO59_04585"
     CDS             528..2213
                     /locus_tag="PLO59_04585"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQV00413.1"
                     /translation="MKKKEVKNKAKLDIKTQDKNWPYLLLLFLITCFAFWPTLNNGFT
                     NWDDPTYLINNLLIKQLNATSIKQMFTEVYFSNYQPLHLLSYAIEYKLFGLDPKGYHL
                     VSILMHAVNACLVFWLIKKLANNQVAFITAALFALSPLRVESVAWASERKDLLYAMFY
                     FAALITYINYVRNQTWGLYLVGTFAFFVLAVFSKAMAVSLVPLLWVFDFYLKRKFDLK
                     CILEKVPFLILALIMGLVSYNASATDGSIDTSDLYTFTDRIFFAAHNFIQYIFKSIIP
                     YKLSAFYQYAPKVTAIYYGAFALVVLLIATSIYLFRASKIFLFTLLFFGCSIFLVLML
                     IPVGPTVFSERYAYIPSVALYFLIAWYAVQFYYQSKKYKAMVQVALVIVLAYFTYVSR
                     IRCQVWNNSIALWSDVLLQFPTAPHALNNRGDAYFVNQQIPEAIADFNLAIAGNNKYA
                     QAYYNRANAYGQQGKADLALKDLNACLKYDTGNAEAYNKRGQAYAILGNVNSALTDFN
                     KALDLKPALTEVYYNLGITYLNTGQKDLACQNLRMALNAGYELAQAPFDNACK"
BASE COUNT          677 a          424 c          460 g          751 t
ORIGIN      
        1 ggctcaagtt caacgagcta gtaagaaaga aaaaggtcaa agcgcccatc gtgattggcc
       61 gtgaccacct cgactgcggc agcgtggcca gccccaaccg cgaaaccgaa gccatgaaag
      121 acggcagcga tgccatagcc gattggcctg ttttaaatgc cttaataaat actgcaggtg
      181 gtgccagttg ggtatcgttg caccatggcg gtggagtagg cattggctac agcatacatg
      241 caggtatggt tatagtagct gatggtacag atgctgcagc aacgcgtatt aagcgtgttt
      301 tgcataacga cccagctatg ggtgttttgc gccacatgga tgccggttat gacatagcca
      361 cagatacagc acgtaagcac cgcttagata tacgagagcg tttgaagtaa tggtggttat
      421 taaagataaa aatgcggctg ttttttagcc gcattttttt tgccttattt gtctgctctg
      481 caatttaaat ttaagccaag ggtattgcat caaatcaaaa ggagctaatg aaaaaaaaag
      541 aagttaaaaa caaagccaag cttgacataa aaacacaaga taaaaattgg ccttatcttt
      601 tattgctgtt tttgattact tgtttcgcct tttggcctac attaaataat ggttttacca
      661 attgggacga cccaacctat ctcattaaca acctccttat taaacaatta aatgcaacca
      721 gcattaaaca aatgtttacc gaggtttact tttcaaatta ccagcccttg catttacttt
      781 cgtatgcaat tgaatataaa ctttttggtt tagatccgaa gggctaccac ttagtaagta
      841 ttttaatgca tgccgttaat gcttgtttgg ttttttggct gataaaaaag ttagctaaca
      901 atcaggtggc atttattact gctgcattgt ttgccttatc gccattacgt gtcgaaagtg
      961 ttgcatgggc atctgagcgc aaagatttgc tgtatgccat gttttacttt gcagccttaa
     1021 ttacgtatat taactatgtt agaaatcaaa catggggatt atacttagtg ggcacttttg
     1081 ctttttttgt tctggcagtt ttttcaaaag caatggctgt atcgctggtt cctttgttat
     1141 gggtgtttga tttttatttg aaacgaaagt ttgatttgaa atgcatactt gaaaaggttc
     1201 cgtttttaat attggcctta attatgggtt tggtatctta caatgcatca gccaccgatg
     1261 gctctattga tacttcggat ttgtacactt ttaccgaccg gatatttttt gccgcacaca
     1321 attttatcca atacatcttt aaatcaatta taccctataa actaagcgca ttttatcagt
     1381 atgcgcccaa agttacagcc atttactatg gcgcttttgc acttgttgtt ttattaatag
     1441 caacgagtat ttatttgttt cgtgcttcga aaatattttt gtttacgctt ttgttttttg
     1501 gatgcagcat ttttttggta ctaatgttaa taccggttgg gccaacagtt tttagcgaac
     1561 gttatgccta tataccatcc gttgccctat attttttaat agcctggtat gccgttcaat
     1621 tttattatca atcaaaaaaa tacaaagcaa tggtgcaggt agctttggta atagttttag
     1681 cttattttac ctatgtatcg cgcattcgct gccaggtgtg gaacaatagt attgcattgt
     1741 ggagcgatgt gttattacag tttccaacag cgccacatgc tttaaataac agaggcgatg
     1801 cttattttgt aaatcagcaa atacccgaag ctattgccga ttttaatttg gctattgccg
     1861 gcaataataa atatgcacaa gcttattaca atcgtgcaaa tgcctatggc caacaaggca
     1921 aagccgattt ggccttaaag gatttaaatg cttgcttaaa atatgatacc ggtaatgccg
     1981 aagcgtataa taaacgtgga caggcgtatg ctattttggg taatgtaaac agtgcattaa
     2041 ccgattttaa taaagcatta gatttaaaac ctgcgttaac agaagtgtat tacaatttag
     2101 gtatcaccta tttaaataca ggccaaaaag atttggcatg ccaaaattta agaatggcat
     2161 taaatgccgg atacgaatta gcacaagcgc catttgacaa tgcctgtaag taaatgaaca
     2221 ctgctgccat aacaaccaaa cgtgcttatt tatacatgca cctaagtatt ttactttggg
     2281 gcttaacagg cattttaggt aaagcaatta ca
//
LOCUS       DAOMAA010000279         2312 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_42945_length_2312_cov_2.223305, whole genome shotgun sequence.
ACCESSION   DAOMAA010000279 DAOMAA010000000
VERSION     DAOMAA010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42945_length_2312_cov_2.223305"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /locus_tag="PLO59_04590"
     CDS             <1..982
                     /locus_tag="PLO59_04590"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQV00414.1"
                     /translation="SYSDKIGKRLPFIQMGYGLTALSRLMLVFFTSISGVFTARLTDR
                     LGKGIRTAARDAILHAEALPQHKATVFGFHRSMDTVGAVLGPLLALIYLYYNPGNYLD
                     LIKWAVLPCFFAVALTFVIKEKPFLVTEKKFSLFPIAYWKTATPAFKTVCLRLWLFAL
                     VNSADVFLLLYLKLHGFTDTQMIAAYLLYNVVFAATAYPIGILADKTSYKPVLFTGLM
                     LFAIVYIGITLTTNLLLLYALMIVYGLYASCFDATAKAFLAIHCKKEEAGSAFGLFGA
                     INSLATLLASVWAGIVWQQGFPATAMVITAMVAFFIAVSFWFRDVKSNQV"
     gene            1031..2008
                     /locus_tag="PLO59_04595"
     CDS             1031..2008
                     /locus_tag="PLO59_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HQV00415.1"
                     /translation="MNISLSEIRKPIELHMEAFEKKFQASMKSNVPLLDKITHYIVKR
                     KGKQMRPMFVFLCAGIHGNINESTHRGAALIELLHTATLVHDDVVDDSNLRRGFFSIN
                     ALWKNKIAVLVGDYLLSKGLLLSIDNGEFTLLQLVSEAVRRMSEGELLQIEKARKLDI
                     EESVYFEIITNKTASLIAACCAVGTASAGANPQQTETMRAIGNDIGIAFQIKDDLFDY
                     MQATQTGKPSGIDIKERKMTLPIIYTLKNATKAERNQIIYIIKNQNEDVAKVAQVLSL
                     INKYQGIEYATKVMEIYCSKALTALSILPDSSYKSALTDLIKYTINRTK"
     gene            complement(2014..>2312)
                     /locus_tag="PLO59_04600"
     CDS             complement(2014..>2312)
                     /locus_tag="PLO59_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014798236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00416.1"
                     /translation="LNNRFDSELDLQGVLFLIGVQELGKGYRQFSKSEKGDLMHIATC
                     KLLSQYGYYVLNGIDVDGWPHWELMQPLPPLTLREQDLLLKQAAIEYFEAEIFG"
BASE COUNT          712 a          417 c          453 g          730 t
ORIGIN      
        1 aagttatagc gataaaatcg gaaaacggtt accatttatt caaatgggtt atggtttaac
       61 tgcattatcc agattgatgc ttgtattttt tacaagcata tcgggtgtat ttacagcaag
      121 gttaacagat aggttgggca aaggcatccg cacggctgca cgcgatgcaa ttttgcatgc
      181 cgaagccttg ccacaacaca aagcaacggt ttttggtttc caccgaagta tggataccgt
      241 tggtgccgta ttaggccctt tacttgcatt aatttatctc tactataatc cgggtaatta
      301 ccttgattta attaagtggg ctgtattacc atgtttcttt gcggttgcat taacttttgt
      361 aatcaaagag aaaccctttc tggtaactga aaaaaagttt agcctatttc caatcgccta
      421 ttggaaaact gcaacacctg catttaaaac agtttgtttg cgcttatggc tatttgcttt
      481 ggtaaacagt gccgatgtgt ttttattatt gtatttgaag ttgcatggtt ttaccgatac
      541 acaaatgatt gcagcctatc tgttgtataa cgtggtgttt gcagcaacag cttatcccat
      601 cggaatttta gccgataaaa caagctacaa acctgtcttg tttaccggct taatgttgtt
      661 tgcaattgtt tatataggca taacgcttac cactaactta cttttacttt atgcgttaat
      721 gatagtatat ggtttgtatg catcctgctt cgatgcaaca gccaaagcat ttttagcaat
      781 acattgcaaa aaagaagagg ccggttcggc ttttggctta tttggtgcta taaatagttt
      841 ggcaacttta ttggcaagtg tatgggctgg tattgtttgg cagcaaggat ttcctgcaac
      901 agctatggta attactgcca tggtagcctt ttttattgca gtaagttttt ggtttcgaga
      961 tgtaaaatca aaccaagttt aggttactat aagggatgca attacattag cccccgctgt
     1021 aaaacaattt atgaatatca gtttaagcga aattcgcaaa cccatcgaat tgcacatgga
     1081 ggcctttgaa aaaaagtttc aagcatccat gaaaagtaat gtgccattgc ttgataaaat
     1141 tacccattac attgttaaac gtaaaggcaa acaaatgcga cccatgtttg tgtttttgtg
     1201 tgcaggcata catggtaata ttaatgaatc aacacatcgt ggtgctgcat taattgaact
     1261 gttacacact gcaacactgg tacacgatga tgttgttgat gactccaatt tacgcagagg
     1321 ttttttttcg attaatgctt tgtggaaaaa taaaattgct gtgctcgttg gcgattattt
     1381 attaagcaaa ggtttgttgc ttagtattga taatggcgag tttacattgc tgcaactggt
     1441 tagcgaagca gtaaggcgta tgagcgaagg cgaactgttg caaattgaaa aagcgcgtaa
     1501 attagatatt gaagagtcgg tttattttga aattataacc aataaaaccg catcgctaat
     1561 tgcagcatgt tgtgccgttg gtacagcttc ggccggagcc aacccgcaac aaaccgaaac
     1621 catgcgcgcc attggaaatg atattggtat tgcttttcaa attaaagatg atttgtttga
     1681 ctatatgcag gccacgcaaa cgggcaaacc atcaggtatt gatattaaag agcgaaaaat
     1741 gacattgcct ataatatata ctttaaaaaa tgctaccaag gccgagcgca atcagattat
     1801 ttatattatt aaaaaccaaa acgaagatgt agctaaagta gctcaggttt taagtttaat
     1861 taataaatat caaggtattg aatatgctac aaaggttatg gaaatttatt gtagcaaagc
     1921 cctaactgcg ctttcaattt tgcccgatag cagctacaaa tcggcactta ccgatttaat
     1981 aaaatataca atcaacagaa ccaaatagga gctttaaccg aaaatctccg cttcgaaata
     2041 ttctattgct gcctgtttca ataataaatc ttgttcgcgc aaagtaagag gtggtaaagg
     2101 ttgcattagt tcccaatgag gccaaccatc aacatcaata ccgtttaata cgtaataacc
     2161 atattgactt agtaatttgc aggtggcaat atgcattaag tcgccctttt ctgatttgga
     2221 aaattgccgg taccctttgc ccaactcttg cacacctatt aaaaacaata caccttgtaa
     2281 atccaattca ctatcaaacc ggttattaag gt
//
LOCUS       DAOMAA010000280         2311 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_42975_length_2311_cov_2.558067, whole genome shotgun sequence.
ACCESSION   DAOMAA010000280 DAOMAA010000000
VERSION     DAOMAA010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42975_length_2311_cov_2.558067"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..1540
                     /locus_tag="PLO59_04605"
     CDS             134..1540
                     /locus_tag="PLO59_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017259712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HQV00417.1"
                     /translation="MKQYLTHPVFHITQQCAHKINMPAYVVGGWVRDLLLHRPCKDID
                     MVCVGDGIALAQAVAKKLGNKTNVTVFKTYGTAQIKLHDVTLEFVGARTESYGAASRN
                     PIVKPGTLLDDQLRRDFTVNALAISLNDDFGALIDAFNGMEHLSQKKLVTPTNPDITF
                     TDDPLRMLRAIRFASQLKFEIAAEALAAINKNAHRISIISQERITDEINKIILSSKPS
                     IGFKLLFDTGLLQLIFPQMVALHGVSTINGKSHKDNFYHTLQVLDNVCKTSDDLWLRW
                     AAILHDIAKPATKRYEPNHGWTFHGHEDKGARMVPGIFKQFKLPLSEPMRFVQKLVKL
                     HLRPIVLAQEIVTDSAIRRLLFEAGDDIDALMKLCTADITSKNEMKVKRFLNNYELVK
                     QKLIEVEEKDRIRNWQPPITGEIIMQTFNLKPSPAIGTIKQIIRDAILDGDINNNYDE
                     AYALMIKTAASLGIKPGN"
     gene            complement(1537..2103)
                     /locus_tag="PLO59_04610"
     CDS             complement(1537..2103)
                     /locus_tag="PLO59_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00418.1"
                     /translation="MVRFVFTVIFFFSAFKVYAQQPPAPLNRGFSKDRLVFGGNVGAQ
                     FGSNTQVNLSPIIGYRLTEKLTTGVGLNYIYYQYKYFGYNGQTFTEKGHIYGGNVWAQ
                     YLIIPQAYVHAEYGILNMPIPKAGANGGYYGETMRYNVNTFLVGGGYFQRIGNNAGVQ
                     FSILWDLIEDQYSPYTNPIIRVGFAGGF"
BASE COUNT          762 a          453 c          420 g          676 t
ORIGIN      
        1 caatggcgaa ataaaaatta ttaggtagta cgaatgctta ccacttgctg aatacttttt
       61 gtataaaaaa ttattgagtt gcataaatgc tatactgcct gcaaatgcta cctatatttg
      121 ataccacatg aatttgaaac aatacctaac gcatcctgtt tttcatatta cacagcaatg
      181 cgcccataaa ataaatatgc cggcctatgt tgtaggtgga tgggtcagag atttgttgtt
      241 gcacagaccg tgcaaagata ttgacatggt ttgtgttggt gatggcattg ctttggcgca
      301 ggcagtagca aaaaaattgg gcaataaaac aaacgttacc gtttttaaaa cgtatggcac
      361 ggcacaaatc aaactgcacg atgttacgct ggagtttgtt ggtgcccgta ccgaaagtta
      421 tggagctgca tcgcgcaatc cgattgtaaa gccgggcact ttacttgatg atcagttacg
      481 cagagacttt acagtaaatg cacttgcaat aagccttaac gatgattttg gtgcattgat
      541 agatgccttt aacggaatgg aacatctatc gcaaaaaaaa ttagtaaccc ctaccaaccc
      601 cgacattacc tttactgatg atcctttgcg aatgttacgc gcaatcagat ttgcatcaca
      661 attaaaattt gaaattgctg cagaggcatt agctgcaata aataaaaatg cacatcgcat
      721 aagtatcatt tcacaagagc gcattacaga cgaaatcaat aaaattattt taagcagcaa
      781 accttctata ggctttaagc tattatttga cacaggttta cttcagttaa tttttccgca
      841 aatggtggca ctgcatggtg taagtaccat taatggtaaa agccataagg ataattttta
      901 ccatacattg caagtactcg ataatgtgtg taaaaccagc gatgacctat ggttacgatg
      961 ggcagcaatt ttacatgaca ttgccaaacc ggcaactaaa agatatgaac ccaatcatgg
     1021 ttggacattt catggccatg aggataaagg tgcccgcatg gtgccaggta ttttcaaaca
     1081 atttaaatta ccattaagcg aacccatgcg ttttgttcaa aaactggtaa aactgcattt
     1141 acgacctatc gtgttggcac aagaaattgt taccgactcg gccatacgca ggcttttatt
     1201 cgaagcaggc gatgatattg atgctttgat gaaactatgc acagccgaca taacttcgaa
     1261 aaatgaaatg aaagtaaaac ggtttttaaa taactatgaa ttggttaaac aaaagcttat
     1321 tgaagttgaa gaaaaagacc gcattcgcaa ctggcaaccg cccattactg gcgaaataat
     1381 tatgcaaact tttaacctta aaccctcgcc tgcaattggt actattaagc aaattattcg
     1441 cgatgcaatt ttagatggcg acataaacaa caactatgac gaagcctatg cattaatgat
     1501 taaaacggct gcatcattag gtattaaacc cggtaattaa aatccaccgg caaaacctac
     1561 tctgataatt gggttggtgt atggcgaata ttgatcttct atcaaatccc ataaaataga
     1621 aaactgaaca ccggcattat tacctatgcg ttgaaaataa ccaccgccca ctaaaaacgt
     1681 gtttacgtta tagcgcatgg tttcgccata ataacctccg ttggccccgg ctttgggaat
     1741 tggcatgttt aaaataccat actcggcatg tacataagct tgcggaataa ttaaatattg
     1801 ggcccaaaca ttaccaccat aaatatgacc cttttcggta aatgtttgac cattgtaacc
     1861 aaagtatttg tactgataat aaatgtagtt taaccccaca ccggtagtta atttttcggt
     1921 taatctataa cctataatgg gcgataggtt tacttgagta ttactaccaa actgcgcacc
     1981 aacattaccg ccaaaaacca acctgtcttt actaaatcca cgatttaatg gtgcaggtgg
     2041 ctgctgagca taaaccttga atgcactaaa aaaaaatatt actgtaaaaa caaaacgtac
     2101 catcttttta ttttatttgt taggcaaact tatcaatttt tactcttcac ttttatcctg
     2161 atttaatcat ttagcatttt caacatataa aacttaataa tcctatgaat attgttatta
     2221 caggtgccag cagtggtatt ggttatcaaa cggcactggc tttttcgtca accattcaaa
     2281 accggattat ttgcatgtcg cgcaatgtgc g
//
LOCUS       DAOMAA010000281         2309 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_43037_length_2309_cov_2.568767, whole genome shotgun sequence.
ACCESSION   DAOMAA010000281 DAOMAA010000000
VERSION     DAOMAA010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43037_length_2309_cov_2.568767"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2187
                     /locus_tag="PLO59_04615"
     CDS             <1..2187
                     /locus_tag="PLO59_04615"
                     /inference="COORDINATES: protein motif:HMM:NF040146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HQV00419.1"
                     /translation="LFLLLQTFAHAQSINVVRSMPTGSTVTVSGIVTNGAEFGNNTRY
                     LQDATAGINGFSNSLSNVQRGDEVTLTGVLYDFNGLLELSPVSLVVVNSNGNTLPQPV
                     SITPTQMGETYESQLVQIQNAVFTNGGQTFNGSTNYTYSANGQSGTIRINGNSNLVGT
                     IIPTQPVSITGICSQFFANYQLLPRDVSDISLSSSLIMTQLPEASNISTTGFRVSWQT
                     NLLPAQSYIVYGKTPALELGMAPAIAGTTSEVFVTGAMPSELYYMRCFSVLGTDTAAS
                     AVGVFITQSVSSGKTICYFNKSVDNSVSTTTPAITLPNTIDDTLIAYINRAKQSIDVA
                     IYNFNQTNLSDIAGALNNAYTSGKTVRLIYNSNTSNTALTLLNPGINLIGSPNAPGYG
                     IMHNKFVVIDAYSNNANDALVWTGSTNWTEGQINDDANNVIIIQDKSLAIAYTLEFNE
                     MYGSPTSTPNTTAAKFGFDKGDNTPHEFVVGGKRLQCYFSPTDNVNNRIIETLNTAQS
                     TCYFALLSFTRFDVASTIDVLTQQIFLTAGIIDDSSGSASTAYNIMSPVMGNKLQVYN
                     HAIHTGVMHHKYAIIDESAVNSDPQVLTGSHNWSSSATQRNDENTVIVHDATIANQYY
                     QEFYKRFYENGGVLAIDNNNSLLQSFILYPNPSNGTFKLQVNLAQVSNIKVTVIDMHG
                     RVVFQNETETTEKIWQQNLTLNVTAGIYVVKVTAGTEVLTQKLLVQ"
BASE COUNT          740 a          449 c          452 g          668 t
ORIGIN      
        1 cttttcttgc tgttacaaac ctttgcccat gcacaaagta ttaatgtggt gcgtagtatg
       61 ccaacaggca gtacggttac cgtatcgggt attgtaacca acggtgctga gtttggcaat
      121 aacacacgct atctgcaaga tgctacagca ggtataaacg gctttagcaa cagtttaagc
      181 aatgtacagc gtggcgatga agtaacacta acgggtgtgc tatacgattt taatggttta
      241 ctcgagcttt cgcctgtatc gttagtggtt gtaaattcga atggtaacac attaccacaa
      301 ccggtatcaa ttacacctac acaaatgggc gaaacctacg agtcgcaatt ggtacaaatt
      361 caaaatgctg tttttaccaa tgggggtcaa acatttaatg gaagtacaaa ttacacttat
      421 agcgccaatg gccaatccgg taccatacgc ataaatggca acagcaattt agtgggcact
      481 ataataccta cacaacctgt tagtattacc ggcatttgct cacagttttt tgcaaactat
      541 cagctattac cgcgcgatgt tagcgatatt agtttatcga gcagtttaat aatgactcag
      601 ttacccgaag catccaacat cagcaccaca ggctttaggg tatcgtggca aaccaattta
      661 ctgccagcac aatcgtacat tgtttatggt aaaacacctg ctttagaatt gggtatggca
      721 cctgcaatag caggaaccac cagcgaagtg tttgtaaccg gtgccatgcc cagcgaattg
      781 tattacatgc gttgtttttc ggttttaggt accgataccg ctgcaagcgc tgtgggtgtt
      841 tttataactc aatcggtaag cagtggcaaa accatttgtt actttaataa gagtgttgat
      901 aacagtgtat ccacaacaac gccagccatt acattaccta ataccattga cgatacttta
      961 atcgcatata tcaatcgtgc caaacaaagt attgatgtag ccatatataa ttttaatcaa
     1021 accaacctga gcgatattgc cggtgcgtta aacaatgctt acacaagcgg caaaaccgtg
     1081 cgcttaattt ataacagcaa taccagcaat acagctttaa ctttattaaa tcccggcatt
     1141 aatttaatag gtagccccaa tgcaccgggc tatggcatta tgcacaataa atttgtagtt
     1201 attgatgctt attcgaacaa tgcaaacgat gctttggtat ggactggtag caccaactgg
     1261 actgaaggac agattaatga tgatgccaat aatgtaataa ttattcagga taaaagtttg
     1321 gctattgctt atacattaga gtttaatgaa atgtatggca gccctacatc aacgcctaat
     1381 acaactgctg ctaaatttgg tttcgataaa ggtgacaata caccgcatga atttgtagtt
     1441 ggtggtaagc gtttgcagtg ttatttcagc ccaactgata atgtaaacaa caggattatt
     1501 gaaacattaa atacagcaca atcaacctgc tattttgcat tgctgtcgtt tacacgtttc
     1561 gatgttgctt ctacgattga tgttttaaca cagcaaatat ttttaacggc tggcatcatt
     1621 gatgattcgt caggcagtgc cagtactgct tataatatta tgagtccggt tatgggcaat
     1681 aaattgcaag tatataacca tgccattcat acaggcgtta tgcatcataa atatgccatt
     1741 atagatgaaa gtgcagttaa cagcgatcca caagtattaa ccggttcgca taactggagt
     1801 agctcggcaa cgcaacgtaa tgatgagaac actgtaattg tgcatgatgc tacaattgcc
     1861 aatcagtatt atcaggagtt ttacaaacgg ttttatgaga acggtggcgt tttggcaata
     1921 gataataata atagcctgtt acaaagtttt atactgtatc ccaatccttc gaacggtaca
     1981 tttaaacttc aggttaattt agctcaagtt tcaaatatca aagtaactgt tattgatatg
     2041 catggccgtg tggtatttca gaatgaaaca gaaacaactg aaaaaatctg gcagcaaaat
     2101 ttaactttaa atgtaacagc cggcatatac gtagttaaag taacagccgg aaccgaagtg
     2161 cttacacaaa aattattagt tcaataaata ctactgtaaa aaatcattaa cggtaccaaa
     2221 catccaattt ggcgattcgg catgcaccca atggcctaca ttgggttcgg ttgcaaatgt
     2281 ggcaaacgga aaaagtatat taattaaat
//
LOCUS       DAOMAA010000282         2305 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_43162_length_2305_cov_4.131111, whole genome shotgun sequence.
ACCESSION   DAOMAA010000282 DAOMAA010000000
VERSION     DAOMAA010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43162_length_2305_cov_4.131111"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="PLO59_04620"
     CDS             complement(<1..554)
                     /locus_tag="PLO59_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010519116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQV00420.1"
                     /translation="MENRADIRALTLQQLQDFFIAQGDKKYRAQQVYDWLWKHAATQF
                     ESMNNLSKPQIEMLQKHFVINNVTVETKQVSNDRTIKNAFKLYDGNVVEGVLIPTETR
                     MTACVSSQVGCSLTCKFCATGKLDRVRNLNADEIFDQVAIIKQQAETHYQMPLSNIVY
                     MGMGEPLLNYANVLKSIAHITSQQG"
     gene            683..1864
                     /locus_tag="PLO59_04625"
     CDS             683..1864
                     /locus_tag="PLO59_04625"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQV00421.1"
                     /translation="MQRYYTANYIFTIDAAPIKDGYLLMDGNTVVAVSSKPIETNIAL
                     QKLEGFIVPGFVNAHCHLELSYLKNQITPGTGLVNFVKNILAIRANYGLTLIKDCIES
                     AYEEMYENGIVAVADISNDDFTFNAKADTRIYFHTLIEAFDIVPAQTKTIFDKCLELT
                     KTLQQLAPNNQAHSIVPHAAYTVTPALFKLIKGHATKHQQLQSMHNQECVAESQMFEN
                     KRGAFYDFFTQHGSMAHFESTGKNSLLSVLPNLSNEKTLLVHNTFTDGNDIAKAMALH
                     KKLFWCFCPNANLYIENRLPDFDVFINAGTTCCLGTDSYASNYTLNLLDEIKTILKFK
                     PHYPFEMLLKWATLNGAQFLGLDYLGSITAGKKPGINLITNCDASTLNITTQSKVKRL
                     H"
     gene            complement(1877..>2305)
                     /locus_tag="PLO59_04630"
     CDS             complement(1877..>2305)
                     /locus_tag="PLO59_04630"
                     /inference="COORDINATES: protein motif:HMM:NF026080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4468 domain-containing protein"
                     /protein_id="HQV00422.1"
                     /translation="TTEGLTKEQLYDKAFTWLMGYYKNPSDVLREKDRETGTMLLKAR
                     FKIFNPTDKKNDVITPAGDVQYSLKLEFRNGRCRYKLTEINWKQPSYYAIERWQDKTQ
                     QSYQPNYDYYLKQTDENCKKIAADFTKAITAVKEVKKDEW"
BASE COUNT          692 a          427 c          408 g          778 t
ORIGIN      
        1 ccctgttgtg aagtgatgtg tgctattgat ttgagaacgt ttgcataatt aagcaaaggc
       61 tcgcccatgc ccatataaac aatgttgctt aatggcattt gataatgcgt ttcggcttgt
      121 tgtttaatta ttgccacctg atcaaatatt tcatcagcat taaggtttcg tactctgtct
      181 aatttgccgg tagcacaaaa cttacaggtt aagctgcaac ctacttgcga tgaaacacag
      241 gcagtcatac gggtttcggt tggaattaat acaccttcaa ccacattacc atcatacaat
      301 ttaaatgcat tttttattgt acggtcgtta cttacttgtt tggtttcaac agttacgtta
      361 ttaataacaa agtgcttttg aagcatctca atttgtggct tgcttaaatt gttcatgctt
      421 tcaaattggg tggcagcatg tttccaaagc caatcgtaca cttgttgggc acggtatttt
      481 ttatcgcctt gtgcaataaa aaaatcctgc aattgctgta aggttaatgc tctgatgtcg
      541 gcccggtttt ccattgttgc aaagataggt tttataacta aattacttgt cccgtagctg
      601 tttgcaatgg tttaaagatt gattaaaccc aaaaaacatg atgtataata ccgtaggttt
      661 gtgttaaatt ttcttgtcaa ttatgcaacg ctattacact gccaactata ttttcacaat
      721 agatgcagca ccaataaaag atggctattt gcttatggat ggcaacaccg tggttgctgt
      781 tagctctaaa cctattgaaa caaatattgc cttacaaaag ttggaaggat ttattgtacc
      841 cggctttgta aatgcacatt gccatttaga actctcctac ttaaaaaatc aaattacacc
      901 gggcaccggt ttggtaaact ttgtaaaaaa cattttagct atcagagcta attatggttt
      961 gacgttgatt aaggattgta ttgaaagtgc ctatgaagaa atgtacgaaa atggcattgt
     1021 ggctgtagcc gacatttcga atgatgattt tacatttaat gccaaagcag atacacgcat
     1081 ttacttccat acacttattg aagcatttga tattgtacct gcacaaacca aaacaatatt
     1141 tgataagtgc ctggagctta ccaaaacatt acagcaatta gcacctaaca accaagccca
     1201 cagtattgta ccacatgcag catataccgt aacccctgca ttgtttaaac ttataaaagg
     1261 ccatgccacc aagcaccaac agctacaaag catgcataat caggagtgtg ttgccgaaag
     1321 tcaaatgttt gagaataagc gtggtgcctt ttatgacttt tttacgcaac atgggtcaat
     1381 ggcacatttt gaatctacag gcaaaaactc attgctatcc gtgcttccta atcttagcaa
     1441 tgaaaaaaca cttttagtgc ataacacttt tactgatggc aatgacatag cgaaagcaat
     1501 ggcattgcat aaaaaattgt tttggtgttt ttgccccaat gctaatttat atattgaaaa
     1561 ccgtttacct gattttgatg tgtttataaa tgccggaact acttgttgcc ttggtactga
     1621 tagttatgcc agtaattata cgttaaatct gttggatgaa attaaaacca tactcaaatt
     1681 taaaccacat tacccgtttg aaatgttatt aaaatgggct acgcttaacg gtgcgcaatt
     1741 tttagggtta gattatttgg gcagcataac ggcaggtaaa aaaccgggta ttaatttaat
     1801 tacgaattgt gatgcatcaa ccttaaacat tactacgcaa agcaaggtta aacgtttaca
     1861 ttaaaaatat caaaggctac cactcatctt tttttacttc tttaaccgct gtaattgctt
     1921 ttgtaaaatc ggcagcaatt tttttacagt tttcgtctgt ttgttttaag taataatcgt
     1981 agtttggctg ataggattgt tgtgttttat cctgccacct ttcaatagca taatagctgg
     2041 gttgtttcca atttatttcg gttaatttat agcggcagcg accgtttcta aattctaatt
     2101 tcaaactata ctgcacatcg cctgcaggtg taattacatc gtttttttta tcagtaggat
     2161 taaatatttt aaaacgtgct tttagtaaca tggtgccggt ttctctgtct ttttcacgca
     2221 aaacgtctga tggattttta tagtatccca ttaaccacgt aaatgcctta tcatacaatt
     2281 gctccttagt taaaccctca gttgt
//
LOCUS       DAOMAA010000283         2305 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_43170_length_2305_cov_3.291111, whole genome shotgun sequence.
ACCESSION   DAOMAA010000283 DAOMAA010000000
VERSION     DAOMAA010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43170_length_2305_cov_3.291111"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="PLO59_04635"
     CDS             <1..894
                     /locus_tag="PLO59_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014938091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HQV00423.1"
                     /translation="YTYFIDDFTSTVSPREILVSDAEKGNKTQYLLIAPNPLKDYYTT
                     PVKLFTIKAADGTDLHCRQFNPIPFDSTKKYPVIVYQYNGPNVQLIFNSWNGGGDYWF
                     QYMADNGYVVFSVDGRGSANRGLAFEQATFRQLGTIELQDQMAGVAYLKSLPYVDANR
                     MGIYGWSYGGFMTTYMMLQNPDVFKAAVAGGPVIDWSFYEVMYTERYMDTPQNNKAGY
                     DANNLTKQVDKLKGHLMLIHGTSDDVVVWQHSINFVKACVDAGKQVDYFMYPGHLHNV
                     TGKDRPHLFQKITDYFNQHLK"
     gene            1128..>2305
                     /locus_tag="PLO59_04640"
     CDS             1128..>2305
                     /locus_tag="PLO59_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002979884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HQV00424.1"
                     /translation="MDNFIYIVPALAIVGLIYTFIKASWVTKQDAGNDRMKEIAGYIA
                     DGAMAFLKAEYRILSIFVLMAALLLGFMGSANPDSHWTIALAFVLGAIFSAAAGFIGM
                     RIATKANVRTASAARTSLSKALAVSFGGGSVMGMGVAGLAVLGLGGLFIVLKNFFGAT
                     TVDSAEMIKTIEVLTGFSLGAESIALFARVGGGIYTKAADVGADLVGKVEAGIPEDDP
                     RNPATIADNVGDNVGDVAGMGADLFGSYVATVLATMVLGRETTSVDNFGGLAPILLPM
                     LIAGMGIIFSIVGTWFVRVSDSATASAAKVQNALNMGNWGSIVITAIFCYFAVNWMLP
                     DTMNLRGYEFTKGGVFAAIIVGLVVGTLMSIITEYYTAMGKRPVNTIVRQSSTGHATN
                     "
BASE COUNT          643 a          447 c          514 g          701 t
ORIGIN      
        1 tacacctatt ttattgacga ttttacaagc accgtatcgc cacgcgaaat tttggtaagc
       61 gatgccgaaa aaggaaacaa aactcagtac ctgctcatag cacccaatcc acttaaagat
      121 tattacacca cacctgttaa actatttacg attaaagcag ccgatggtac cgatttacac
      181 tgccgtcaat tcaacccaat tccattcgat tcaacaaaaa aatatccggt aattgtttac
      241 caatacaatg gccccaatgt gcagctcata tttaatagct ggaacggtgg tggcgattac
      301 tggtttcaat acatggccga taacggatat gttgtttttt cagttgatgg ccgcggaagt
      361 gccaatcgcg gattggcttt cgaacaagct actttcaggc aattaggtac aattgagctg
      421 caagaccaga tggcaggtgt ggcctactta aaatcattac cttatgttga tgccaatcgc
      481 atgggtatat atggatggag ttatggtggg tttatgacta cttacatgat gttgcaaaac
      541 cctgatgtgt ttaaagcagc cgtagccggt ggacccgtta ttgactggag cttttacgaa
      601 gtaatgtaca ccgagcgata tatggatact ccgcaaaaca acaaggccgg ttacgatgcc
      661 aataatttaa ccaaacaggt tgataaactt aaaggccacc tgatgcttat acatgggaca
      721 agcgatgatg tggtggtatg gcaacacagt attaattttg taaaagcctg tgtggatgca
      781 ggcaaacagg ttgattattt tatgtatcca ggccatttac acaatgttac cggcaaagac
      841 cgaccacatt tgtttcaaaa aataaccgat tactttaacc aacatcttaa gtaatacctg
      901 taagaactgt ttaccactat cggcccaact tctaaggtgc attggtttat tcaagcaaaa
      961 accttgcatt atctgtttag cttaaaaagg cacttaggac tctaacaatt gcctgtaata
     1021 aaaccggtaa aatggtctat aatacgtgtg tttatgggca ttgatatttt ttttgctccc
     1081 aaactcaaaa gttctacttt cgcgcaccca aatttaaaaa agtttatatg gacaatttta
     1141 tttacatcgt tcctgcttta gctattgtag gtttgattta cacttttatt aaggcaagct
     1201 gggtaacaaa gcaagatgcc ggcaacgaca ggatgaaaga aattgcaggt tacattgccg
     1261 atggtgctat ggcattctta aaagcagagt atcgcatcct ttctattttt gttttaatgg
     1321 ctgcattgct tttaggcttt atgggcagtg ccaaccccga tagtcattgg actattgcat
     1381 tagcatttgt attaggagct attttttcgg ctgcagccgg atttattggc atgcgcatag
     1441 caaccaaggc caatgtgcgt acagcaagtg cagcacgtac cagtttatca aaagcactgg
     1501 ctgtttcatt tggtggaggc tcggttatgg gtatgggtgt tgccggttta gctgttttag
     1561 gtttaggcgg attatttatt gtacttaaaa acttttttgg tgcaactacg gttgatagtg
     1621 ccgaaatgat aaaaaccatt gaagttttaa ctggcttttc attaggtgcc gaaagtattg
     1681 cactttttgc ccgtgttggc ggtggtattt ataccaaggc agccgatgta ggtgccgatt
     1741 tagttggtaa agttgaagcg ggtattcccg aagacgatcc acgtaacccc gcaaccattg
     1801 ccgataacgt aggcgataac gtgggcgatg tggcaggtat gggtgccgat ttgtttggct
     1861 cttatgttgc aaccgttttg gcaaccatgg ttttaggccg cgaaacaact tcagtagata
     1921 attttggcgg attagcccct attttattac ccatgttaat tgccggtatg ggcattattt
     1981 tctctattgt aggtacttgg tttgtacgtg taagtgatag cgcaaccgca tcggcagcca
     2041 aagtgcaaaa cgcattaaat atgggcaact ggggtagcat cgttattacg gctatattct
     2101 gctactttgc cgttaactgg atgttgcccg ataccatgaa cctgcgtggt tatgaattta
     2161 ctaaaggtgg cgtatttgca gcaattattg ttggcttagt ggtgggtacg cttatgagta
     2221 taattaccga gtattacaca gctatgggaa aacgtccggt aaatacaatt gtacgtcaat
     2281 cgtcaaccgg ccatgctact aatat
//
LOCUS       DAOMAA010000284         2302 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_43277_length_2302_cov_3.026257, whole genome shotgun sequence.
ACCESSION   DAOMAA010000284 DAOMAA010000000
VERSION     DAOMAA010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43277_length_2302_cov_3.026257"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2010
                     /locus_tag="PLO59_04645"
     CDS             <1..2010
                     /locus_tag="PLO59_04645"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV00425.1"
                     /translation="CTSIAGGATPTRCNYSYLGVTGLNSNAVNNAPAPYGVNYDFAYE
                     RRLAVNAGDIIKLVVSNFSNSTSGFTMDFLASPINYSAAPGFVVWTGGIDDDWFKIEN
                     WGGCAIPTCAIDAIIPPSSANMPAIYRNGAVCKSLQINPSSSLTLHTGYTLQVCGNYT
                     NNGLFTAQPNSTVLINNANVVQQLNGTMVGTSAFNHLTINKTGGSVVFNQAVDNTGNF
                     TATTGTSVCNAQGFYHRVAGNFNVAGTYTTGTNTLEFNGSAAQNYNYVGDVNHVIMQH
                     TGPGLTLSSNLNLATTGTLQLNNGRIITTPAYEVYIKNIAPASCNIGNANSFVLGFLR
                     RNLSSLGAFNFPVGHSTAGYQLALINFNYAAAPTTIGNLQASFELHPTLPPGIGGFEC
                     AVSYSQPALNNGRWKFVANNNPTSGNFDATLYNTNYSNAASGFTVMRNPNTTWNLYNG
                     VCTPCPVTAVGRLNMNGFGTGTSYFGVAQAPTPLPVSLILFEAKAATQSIVVRWVTVS
                     ENQVSNFELNRSIDGVNFMPITHMQPMGNIQSGHTYNYIDKDVEPGVLYYYKLKTNDI
                     NGQFTISHIEKAVTEFNVTDFAATPNPFSDFTTISFNLKELSDVRLDVLNQFGATVFI
                     LENTTLKKGQYSYRFDAKKAGLSKGLYCIRLITNNQTSTKKVIFE"
BASE COUNT          717 a          460 c          430 g          695 t
ORIGIN      
        1 tgcacatcta ttgctggtgg tgcaacacct acacgatgca attactctta cttaggtgta
       61 acaggcctta acagcaatgc tgttaataat gctcctgcac cttatggtgt aaattatgat
      121 tttgcctatg aacgcaggct tgccgttaat gctggcgaca ttataaaatt agtggtaagc
      181 aatttttcga atagcacatc aggttttacg atggattttt tggcctcgcc tataaattat
      241 tcagcagcac cgggttttgt tgtttggacc ggtggcatag atgacgattg gtttaaaatt
      301 gaaaactggg gagggtgtgc tatacccacc tgtgctattg atgccattat acctccatca
      361 tcggcaaata tgcctgccat ttatagaaac ggtgctgtat gtaaatcatt acaaattaat
      421 ccatcatcaa gccttacttt acacacgggc tataccttac aagtttgcgg caactatacc
      481 aataatggct tatttacagc acaaccaaac agcaccgttt taattaataa tgccaatgtg
      541 gtacagcagt taaatggaac gatggtaggt acttcggctt ttaatcactt aaccataaat
      601 aaaaccggtg gtagcgttgt atttaatcag gcagttgaca ataccggaaa ttttacagct
      661 actacaggca catctgtttg taatgcgcaa ggattttacc acagggttgc aggtaatttt
      721 aatgttgccg gcacttatac tacgggtacc aatacccttg aatttaatgg tagtgcagca
      781 caaaattaca attacgttgg cgatgttaac catgtaatta tgcaacatac aggccccgga
      841 ttaacattaa gcagcaacct taatttggct acaacaggca cactgcaatt aaataacggt
      901 agaattataa ccacacccgc atacgaagta tatattaaaa acattgcgcc tgcatcttgc
      961 aatattggta acgcaaatag ttttgtatta ggttttttaa gacgcaacct gagtagttta
     1021 ggtgccttta attttccggt tggtcatagt acagccggct accagttggc actaataaat
     1081 tttaattacg cagcagcgcc cacaaccatt ggcaacttac aagcaagttt tgagttgcat
     1141 cctacattac cacctggcat aggcgggttt gagtgtgccg tatcgtatag ccaacccgca
     1201 ttaaataatg gccgatggaa atttgttgca aacaacaacc caacatcagg caactttgat
     1261 gcaacactgt ataacaccaa ctatagcaat gcggcatcgg gctttacggt tatgcgtaac
     1321 ccaaacacca cctggaattt gtataacggt gtttgtacgc catgtccggt aaccgctgta
     1381 gggaggttaa acatgaatgg ttttggcaca ggcacttctt attttggtgt agcacaggct
     1441 ccaacaccat tacccgttag tttaatttta ttcgaagcca aagcagctac acaaagcatt
     1501 gttgtacgtt gggttacagt ttccgaaaat caagttagca attttgaatt aaaccgcagt
     1561 atagatggtg taaattttat gcccataaca cacatgcagc caatgggtaa tatacaaagc
     1621 gggcatactt ataactatat tgacaaggat gttgagccgg gcgttttata ttattacaaa
     1681 cttaaaacca atgacattaa tggccagttt actattagtc atatcgaaaa agccgttact
     1741 gaatttaatg ttactgactt tgctgcaaca cctaatccat tcagcgattt tacaaccatc
     1801 tctttcaact tgaaagaact ttcagacgta aggttagatg tgcttaatca atttggcgca
     1861 actgtattta tcctcgaaaa cacaacgctt aaaaaaggtc agtacagtta tcgttttgat
     1921 gccaaaaaag cgggcttatc aaaagggttg tattgcatca ggcttattac aaacaatcaa
     1981 accagtacta aaaaggttat ttttgagtaa tagcatctgt aggcaatttg taataagcat
     2041 tagcttaagt aagcttttat taataatttg ataacattgc ttaaactttt gatgcaataa
     2101 gcaatccaat gcttcttgta attattggta aaaccacttt tgataaaccc cttaaaccaa
     2161 ctattttgtt ttatgaaaca tctttttaat gccattgttc ttgctttgat atttcaatat
     2221 accacacaag cgcaacaatg gggcaactac acgttgtaca gtacttctaa tggcaccagc
     2281 acgtatttga tagatacaaa ct
//
LOCUS       DAOMAA010000285         2298 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_43421_length_2298_cov_2.907713, whole genome shotgun sequence.
ACCESSION   DAOMAA010000285 DAOMAA010000000
VERSION     DAOMAA010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43421_length_2298_cov_2.907713"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="PLO59_04650"
     CDS             complement(<1..802)
                     /locus_tag="PLO59_04650"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HQV00426.1"
                     /translation="MKNLLLLITLLIAFGNTQAQDAFELPPEWSKNAIWYQIMVERFH
                     NADTANDPKPDNMLAPNEGAIAPTGWHITPWAQNWFKADDWMVNNKLDLKHAFGYRRY
                     GGDLQGVIDKLDYLVDLGITAIYLNPVNDAPSLHKYDARSYHHVDVNFGPTPDTDTKL
                     MATENPADASTWVWTNADKLFLKLISEAHNRNIKVIVDFSWNHTGTEFWAWKDIIEKQ
                     EKSAYKDWYYIQTFDNPATTQNDMIYKGWANLKGLPELRKSNIVSARKS"
     gene            complement(863..2053)
                     /locus_tag="PLO59_04655"
     CDS             complement(863..2053)
                     /locus_tag="PLO59_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010599726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00427.1"
                     /translation="MITEVRKKYNSLFTDAQYQAFLNHLDVLYEKPVGFRVPETPIFI
                     DKALKQKLINAGEQIIDVILQPDFKERTERAIPPQYNVKNENQHPHFLAIDFGICKDA
                     EGNFTPQLIEMQGFASLYVWEQLIGRKYKEFFYCPDGYTPFFNGLNNQTYLDLLRKVI
                     IGSYQPEQVVLMDINAHEQKTRIDFFATKEALGIPIVCLSELKQEGKKLYHTLNNKAI
                     EVKRIYNRLIFDDLQANPDFKYSINLFDDLDVEWITHPNWFYRISKFTMPMVKSDFVP
                     ETIFLNTLQTIPADLENYVLKPLFSFSGMGVLIDVTPADIEKITDKENWILQRKVQYA
                     DAVQSPEGGVKCEIRLMYLWPDGDKRPTLAVNLARMSRGKMIGVRYNKDFTWVGGNIA
                     FMQP"
     gene            complement(2050..>2298)
                     /locus_tag="PLO59_04660"
     CDS             complement(2050..>2298)
                     /locus_tag="PLO59_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013407904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase"
                     /protein_id="HQV00428.1"
                     /translation="IQFTPEIFGTQFHPEADADGMILYLNNAEKKKLVIDNYGLKKYN
                     DMLNHLYDPDKIKLTQSLIIPKFLDEALAQIKNLMLVL"
BASE COUNT          643 a          424 c          444 g          787 t
ORIGIN      
        1 cacttttacg tgcactaact atatttgatt tacgcagctc gggtaggcct ttcaaattag
       61 cccatccttt gtaaatcatg tcgttttgtg ttgttgccgg attgtcaaat gtttgaatgt
      121 aataccaatc tttatatgcc gatttttctt gcttttcgat aatatctttc caagcccaaa
      181 actctgtacc ggtatggttc caactaaaat caactatcac tttaatattt ctgttgtgag
      241 cttcgctaat aagttttaaa aataatttgt cggcattggt ccaaacccag gtacttgcat
      301 cggccgggtt ttcagtggcc attaattttg tatcggtatc gggtgtaggg ccaaaattta
      361 catctacgtg gtgatagctt cgtgcatcat atttatgcaa tgaaggggca tcattaaccg
      421 ggtttaagta gatggcagta atgcctaaat caactaaata atctaacttg tcaattacac
      481 cttgcagatc gccaccgtag cgtctgtaac caaaagcgtg ctttaaatca agtttgttgt
      541 taaccatcca atcgtctgct ttaaaccagt tttgtgccca gggggtgatg tgccaacctg
      601 ttggtgctat tgcaccttcg tttggtgcca gcatattatc aggcttaggg tcgttagctg
      661 tatcggcatt atgaaaccgt tctaccataa tctgatacca gattgcattt ttactccatt
      721 cgggcggtaa ctcaaaggca tcctgagctt gggtattacc aaatgctatg agtaaggtta
      781 taagcagaag caagttcttc atttcctaaa ttgaaataaa ttaaaatgaa attttaaatg
      841 tgtgtttcgt atatggggtc tgttagggct gcataaatgc aatgttgcca cctacccagg
      901 taaagtcctt attatagcgt acaccaatca ttttgccacg actcatacgt gctaaattta
      961 ctgccaatgt gggtcgctta tcaccatcgg gccataaata cattaacctg atttcgcatt
     1021 taacaccacc ctccggactt tgcacggcat cggcatattg taccttgcgt tgcagtatcc
     1081 aattttcttt gtcggttatt ttttcaatgt cggcaggcgt tacatcaata agtactccca
     1141 tacccgaaaa actgaataac ggtttgagta cataattttc taaatcggct ggtatggttt
     1201 gtagtgtatt caggaaaatg gtttcaggta caaaatcact tttaaccatg ggcatggtaa
     1261 acttgctgat gcgataaaac caattgggat gtgttatcca ttcaacatcc agatcgtcaa
     1321 acaaattaat gctgtattta aaatcgggat tggcttgtaa atcatcaaaa ataagacggt
     1381 tgtaaatccg ttttacttca atggctttat tattaagtgt atgatatagt tttttgcctt
     1441 cttgctttaa ttcactcaaa caaactatgg gtatgcctaa tgcttcttta gttgcaaaaa
     1501 agtcaatgcg tgttttttgt tcatgggcat taatgtccat taaaactact tgttcgggtt
     1561 ggtaagagcc tattataact ttacgtaaaa gatcaagata ggtttgatta ttaaggccat
     1621 taaaaaatgg cgtgtatcca tcggggcaat agaagaactc tttgtattta cgtccaatta
     1681 attgctccca aacataaagg ctggcaaagc cttgcatttc gataagttgg ggtgtaaagt
     1741 ttccttcggc atctttgcaa attccaaaat caatggctaa aaagtgtggg tgttggtttt
     1801 cgttttttac gttgtattgt ggcggtatgg cacgttcggt acgttcttta aaatcaggtt
     1861 gcagtataac atcaataatt tgctccccgg cattaattaa tttttgtttt aatgctttat
     1921 caataaaaat gggcgtttcg ggcacgcgga atcctacagg cttttcgtac aaaacatcta
     1981 aatgatttaa aaatgcctga tactgtgcat cggtaaaaag gctgttgtat ttttttctga
     2041 cttctgtaat cataatacca acatcaggtt tttaatttgt gcaagggctt cgtccaaaaa
     2101 cttaggaatt ataagacttt gtgtaagctt aattttatct gggtcgtaca aatggtttaa
     2161 catgtcgttg tatttcttta aaccatagtt atcaataacc aatttctttt tttcggcatt
     2221 attcagatag agaatcatac catcggcatc ggcttcggga tgaaattgtg tgccaaaaat
     2281 ttcaggtgta aattgtat
//
LOCUS       DAOMAA010000286         2296 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_43502_length_2296_cov_2.224007, whole genome shotgun sequence.
ACCESSION   DAOMAA010000286 DAOMAA010000000
VERSION     DAOMAA010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43502_length_2296_cov_2.224007"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="PLO59_04665"
     CDS             complement(<1..201)
                     /locus_tag="PLO59_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019973561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00429.1"
                     /translation="MNKSLATALKSFYKVLVFICCPIMLWAQGVGINNATPHASAMLD
                     IVSNNKGLLIPRITTAQRTAIAA"
     gene            complement(244..1836)
                     /locus_tag="PLO59_04670"
     CDS             complement(244..1836)
                     /locus_tag="PLO59_04670"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV00430.1"
                     /translation="MAKIKQMYLAATVVLIVCFAHTCGIAQLLTLNNVAVTVTGATLV
                     TNQGDILLNNGGIFNNNGTIDLTGHFTNNSLTNGFAATFPSGTVICNGAAQNIGGNSY
                     TIFNNLTLTGTGNKTLLQDIGVGGNTTTNGVLALNDRQLLLNSFDLTVRNPLPVAITR
                     TTGFIVSETPPATGYGVLTWNISNGTVGNNYTFPFGNNTTSNYLPLNFNVTTSGVGAT
                     GSVSIATYPTNTALNPNNRPLPTGLTALINNSGADNSAFVVDRYFMFNVANYTTLPVT
                     TMVFPYRDLEWNTGTNTIIENNLRMQRLNNGTQWTQPPFGIVNTALNTVTVTGQNNYS
                     PIWTIVDLTTPLPITLLDFTAKLNQNNYTILNWITANELNSAYFDIEKSTDGQHFSFL
                     HKVMAAQFSNSTLQYHDIDSNPAKGFTYYRIKMVDNNGDFKYSNTQFVYTNLQNATVS
                     VWPNPATNFINITISDAETPYQSFAFVNGIGQTVFQADLKTYQDTKNNYTIPCNNLPS
                     GLYYLQLSNGVGKPFACKIFIQ"
     gene            complement(1941..>2296)
                     /locus_tag="PLO59_04675"
     CDS             complement(1941..>2296)
                     /locus_tag="PLO59_04675"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV00431.1"
                     /translation="NSSWNLGIKRNNTLTCPPNTCSKMDVSFDESDETFFALTAYPNP
                     TEAIFNLSFKTTYATEYRINMIDMKGSQVINEGHVSHAGANELIYNFENLKPGLYMLQ
                     VTIGNEAKTIRVVIQ"
BASE COUNT          727 a          381 c          410 g          778 t
ORIGIN      
        1 cgctgcaatt gcagttcgtt gtgcagtagt tatgcgtggt atcagaagcc ctttattatt
       61 gctcactata tctaacattg ccgaagcatg tggcgttgca ttgttaatac ctacaccttg
      121 tgcccataac ataatcggac aacatataaa tactaatacc ttataaaaag attttaaggc
      181 cgttgctaaa ctcttattca ttaaaccaat tgatatttga aagggaatta tgagttactc
      241 aaactattga ataaaaattt tacaggcaaa aggtttgcca acaccatttg ataattgcag
      301 ataatacaaa cccgatggca aattattaca agggattgta taattgtttt tggtgtcttg
      361 ataggttttt aaatcggctt gaaatacggt ttgacctatg ccgtttacga atgcaaaact
      421 ttgataagga gtttctgcat ctgaaatggt tatgtttata aaattggttg ccgggtttgg
      481 ccaaaccgac acggttgcat tttgcaaatt ggtgtaaaca aattgtgtgt ttgaatactt
      541 aaaatcacca ttattatcta ccattttaat ccggtaatat gtaaaccctt ttgcaggatt
      601 tgaatcaatg tcgtgatatt gaagtgtact attagaaaat tgagcggcca taactttatg
      661 taaaaaagaa aagtgttgtc cgtctgttga tttttcaata tcaaaataag cactatttaa
      721 ttcgtttgct gttatccagt ttaatattgt ataattattt tggttaagct ttgccgtaaa
      781 atctaataat gttataggta aaggcgtggt taaatcaaca attgtccaaa tagggctgta
      841 gttattttga ccagtaactg taactgtatt taaagctgta tttacgattc caaaaggtgg
      901 ttgtgtccat tgtgttccat tgtttaacct ttgcatacgt aaattatttt caataatcgt
      961 attggtaccc gtattccatt ctaagtcacg atatggaaac accatagtag ttacaggtaa
     1021 tgttgtatag tttgccacat taaacataaa atatctatca acaacaaaag ctgaattatc
     1081 tgcaccacta ttatttataa gtgctgttaa tccggttggc aaaggtctgt tgttaggatt
     1141 taaagccgtg ttggtaggat aggttgcaat tgaaaccgaa ccggtggcgc caacgccact
     1201 cgttgtaaca ttaaaattaa gtggcaaata gtttgaggtt gtattgttgc caaaagggaa
     1261 ggtgtaatta tttcctactg ttccattact aatattccaa gttaatactc catatcctgt
     1321 agccggtggg gtttcgctca caataaaacc agtggttcgg gtaatggcaa ccggtagcgg
     1381 attgcgtact gttaagtcaa acgaatttaa aagtaattgt ctgtcgttga gggccaaaac
     1441 accattagtg gttgtgtttc cacctacacc tatatcttgc aaaagtgttt tattaccagt
     1501 gcctgttaag gttaaattat taaatattgt ataggaattg cctccaatat tttgagctgc
     1561 accattacat attaccgtac cacttgggaa ggttgcagca aaaccattgg ttaagctatt
     1621 gtttgtaaaa tggcctgtta aatcaatggt accattatta ttaaaaatac cgccattatt
     1681 taaaagtata tcaccttgat ttgtaactag ggtagcaccg gttactgtaa cagctacatt
     1741 atttagtgtt agcaactgag caataccaca tgtgtgagca aaacatacta tcaacacaac
     1801 tgttgctgcc aaatacattt gtttaatttt tgccattgta aattcaatta aactaaaact
     1861 ttaatactac aaaagtaatt aaataaaaag gagattgtat taaaatacaa tctccttaaa
     1921 gtgtcggaac caaattttat ttactgaata accactctaa tagttttagc ctcattacca
     1981 atggtaactt gtaacatata caatcccggc ttcaaatttt cgaaattgta aatcagttca
     2041 ttagcaccgg catggcttac gtgaccttcg ttaataacct gactaccttt catatcaatc
     2101 atatttatgc gatattcagt tgcataggtg gttttgaagg ataaattaaa tatggcctct
     2161 gttggatttg gataggctgt taatgcaaag aatgtttcat cactttcatc aaacgacaca
     2221 tccattttac tgcaagtatt tggcgggcat gttaaagtgt tattacgctt aatacctaaa
     2281 ttccatgaag agttta
//
LOCUS       DAOMAA010000287         2295 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_43529_length_2295_cov_3.076786, whole genome shotgun sequence.
ACCESSION   DAOMAA010000287 DAOMAA010000000
VERSION     DAOMAA010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43529_length_2295_cov_3.076786"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1779
                     /gene="asnB"
                     /locus_tag="PLO59_04680"
     CDS             <1..1779
                     /gene="asnB"
                     /locus_tag="PLO59_04680"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HQV00432.1"
                     /translation="YANQMLHTTTHRGPDATDTWQQQHLILGHNRLSIIDLSTHANQP
                     MHYAHATLVFNGEIYNYIELRNQLKQAGYSFTTQSDSEVILAAYLYWGHQCVNYFVGM
                     WAFVLYDAKQHQLFIARDRFGIKPFNYILTNNRFYFSSEYKALKQMPVYNGAVNYTQA
                     LRGLQLGWLCYKDETYLQCIKSLPAAHYMILHLHQQLQPEIKCYWQIEPSKIIASFQD
                     KVQMFKQAFADSIQLHMRSDVTVGSCLSGGIDSSAIVSMVQHLNKSMPYKSFSIYYDG
                     KGDVDERPFINEVLHKYPAIEAYFKKPTDDEVAAHFHNALYHADVPATGSSFISQYFL
                     MQLIASQKIKVVLDGQGSDEYLGGYMHTYYRLIADMLRKNEYSSAFSTTKQITNQFGF
                     NFKQKAIHFAKSALCTIRTEQQLYEFEYKNYFPFLGRAIHDAPFQLDAHAGMDGFLIN
                     LIQTTSLPSLLHYEDRNSMAFSIESRVPFLDHRLVELAFSFQNDDKIQGLSTKHILRE
                     ALRDVLPKAITERKDKKGFVTPGENKWLRGPLQHLLQIDYDVLSFLNVDKVKTIVNQY
                     SKGDNSNAVLVWRIATLHYWLKHFVH"
     gene            complement(1957..>2295)
                     /locus_tag="PLO59_04685"
     CDS             complement(1957..>2295)
                     /locus_tag="PLO59_04685"
                     /inference="COORDINATES: protein motif:HMM:NF021246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(R)-hydroxyalkanoic acid synthase subunit
                     PhaE"
                     /protein_id="HQV00433.1"
                     /translation="NTSDKALVALYESDAYSKIQAEVAALAHGIKKDVEVIMEKMMVN
                     IPVITRTEMDDTYKTIYDLKKRVKELERSLESKSVAKAVAVETEMPEVAKAAPTKSVA
                     KAPVKKTGRK"
BASE COUNT          738 a          423 c          408 g          726 t
ORIGIN      
        1 tatgccaatc aaatgctgca taccacaacg caccgtggcc cagatgcaac cgatacctgg
       61 caacagcagc atctgatatt aggtcataac cgtttaagta ttattgattt aagtacgcat
      121 gccaaccagc ccatgcacta tgcacatgca accctggtgt ttaatggcga aatttataat
      181 tacatagagc tcagaaatca acttaaacaa gcgggttata gttttacaac acaatcagac
      241 agcgaagtaa ttttagcagc atatttatac tggggccacc aatgtgtaaa ctactttgtg
      301 ggcatgtggg catttgtatt atatgatgca aaacaacatc agctttttat tgcccgcgat
      361 aggtttggta ttaaaccttt taattacatc ttaaccaata accggtttta cttttcgagc
      421 gagtataaag cccttaaaca aatgcctgtt tataatggcg cggtaaatta cacacaagct
      481 ttaagaggtt tgcaattagg ctggctttgc tataaagatg aaacctattt gcaatgcata
      541 aaatcattgc cagcagcgca ttatatgatt ttgcatttac accaacagtt acaacccgaa
      601 ataaaatgtt attggcaaat agaaccttca aaaattattg cctcgtttca ggataaagtt
      661 caaatgttta agcaggcatt tgccgatagc attcaattac acatgcgcag cgatgttacg
      721 gtaggcagtt gtttaagtgg aggtattgat agtagtgcca ttgtaagcat ggtacagcat
      781 ttaaacaaaa gtatgccata caagtcgttc agcatatatt acgatggcaa aggcgatgtg
      841 gacgaaagac ccttcataaa tgaagtactg cataaatatc cggcaataga agcctatttt
      901 aaaaaaccaa cagatgatga agtggctgca cattttcaca atgcacttta tcatgctgac
      961 gtgcctgcaa ccggctcttc atttatttcg caatattttt taatgcaact tattgcatca
     1021 caaaaaataa aagttgtgtt agatgggcaa ggaagcgatg aatatttagg aggttatatg
     1081 catacctatt accgtttaat tgccgatatg ttgcgcaaaa atgaatacag cagcgcattt
     1141 agtacaacca aacaaattac caatcaattt ggctttaatt tcaaacaaaa ggccatacat
     1201 tttgccaaat cagctttatg caccattcgg acagaacagc aactttacga atttgaatac
     1261 aaaaattatt tcccattttt aggtagagca atacatgatg cgccatttca attagatgca
     1321 catgcaggta tggatggttt tttaatcaac ctcattcaaa caaccagttt gccatcactg
     1381 ttacattatg aggatagaaa cagtatggct ttttcaatcg aatcgcgtgt gccatttctc
     1441 gatcatcgtt tggttgagtt ggcttttagt tttcaaaatg acgataaaat acaagggctg
     1501 tcaaccaaac atattttgcg tgaagcattg cgcgatgttt tgccaaaagc tattactgaa
     1561 agaaaggaca aaaagggttt tgtaacaccg ggcgaaaaca aatggctgcg cgggcctttg
     1621 caacatttgc tgcaaataga ttatgatgtt ttatcgtttc taaatgttga caaggttaaa
     1681 accatagtta atcaatacag taagggcgat aatagtaatg cagttttagt atggcgtatt
     1741 gctacattgc attattggtt aaaacatttt gtgcactaag ttataattca agttcgagct
     1801 aattatttac tgcaattttt aattgcgtat atcagttaaa tcaaatacgc ttttatactt
     1861 tgaaaaaagt gtaagtagtt gcctaaaata aaaaagccac ctctgtatag agatggctaa
     1921 acaaactaca acaaaaaata tttttattga attagattat ttacgtcctg ttttttttac
     1981 aggtgcttta gctacacttt tagttggcgc tgcttttgca acttcaggca tttcagtttc
     2041 aacagcaact gcttttgcaa ccgatttgct ttctaaactg cgttctaatt ctttaactct
     2101 tttctttaaa tcataaatgg ttttataggt atcatccatt tcagtacgtg taattaccgg
     2161 aatgttaacc atcatttttt ccattatcac ttcaacatct tttttaatac catgagctaa
     2221 tgcagcaacc tcagcctgta ttttgctgta agcatcgctt tcgtataaag caactaaagc
     2281 tttatcgctg gtgtt
//
LOCUS       DAOMAA010000288         2294 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_43570_length_2294_cov_2.638231, whole genome shotgun sequence.
ACCESSION   DAOMAA010000288 DAOMAA010000000
VERSION     DAOMAA010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43570_length_2294_cov_2.638231"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="PLO59_04690"
     CDS             complement(<1..668)
                     /locus_tag="PLO59_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00434.1"
                     /translation="MRKLWSVFVLSIGVILGKVYAQHEYILLSKDIIQRYEPYTDAVG
                     SAVHTSFKPLYSADIVTVAPYDSLNTKSIKSTKFNQSWWGRKLHNEHLLQYKKDDVYL
                     YADFLFNFSLTNEFQNNETYVTNSRGVMAGGTLGKGFSFNSYFFENQIAYPTYIDSSI
                     KVNGVIPGQGRGKNFNSSKDFAFAAAHVAYATKKYFKFQLGTDKNFIGEGYRSLLLSD
                     YAFY"
     gene            complement(661..1788)
                     /locus_tag="PLO59_04695"
     CDS             complement(661..1788)
                     /locus_tag="PLO59_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HQV00435.1"
                     /translation="MPFIYLDYNATTPVNEVVVQAMLPFFTDNFGNASSATHQAGWYA
                     KQATENARQQVATLIGAQPDEIIFTSGATEAVNIALQGIYHAYKTKGNHIVTVTTEHK
                     AVGDTCRYLETIGANITFVPVNTHGQLDLNALTQAITPNTIAVCVMLANNETGVLQPV
                     KEIAEIVHKYHCLMVTDATQAVGKIPVDVNTLAVDVLVCSAHKMYGPKGCGALYIRRK
                     NPRVHLKPLLFGGGHEKNIRPGTLNVPGIVGMGAAAALAQTNLTAQMQHLNHLLHYFE
                     DALHQLPVQINGNKVPRLPNTSSVTFTDTTATQLYKALPQIGVANGSACTSALQQPSH
                     VLQAMGLTADLAQTTLRFSFGVPTTIQQLNDVICLIKKMYA"
     gene            complement(1833..>2294)
                     /locus_tag="PLO59_04700"
     CDS             complement(1833..>2294)
                     /locus_tag="PLO59_04700"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HQV00436.1"
                     /translation="ENAIAITLTPEAQKLSNNFSGNRGTLPWPVEQGTITEKFGAHAH
                     PTLKGVVVKNDGVDITTNNQAGVRTIFTGQVTAVISLPGSNNAVIVRHGEYLTVYANL
                     ETVAVSKGEQLTTRQKIGVAGNDSETGKGMINLQIWKGFEKLNPQLWLAKK"
BASE COUNT          617 a          454 c          477 g          746 t
ORIGIN      
        1 taataaaagg catagtcgct aagcaaaagc gaacggtaac cctcgccaat aaaattttta
       61 tcggtgccta attgaaactt aaaatatttt ttagtggcat aagctacatg ggcagcagca
      121 aatgcaaaat ctttactgga gttaaaattt ttgccacgcc cctggccggg tattacacca
      181 tttactttaa ttgaactgtc aatgtatgta gggtaagcaa tctggttttc gaaaaagtaa
      241 ctgttaaatg aaaacccttt gcctaaagtg cctccagcca ttacaccacg cgaattggta
      301 acatacgttt cattgttttg aaactcattg gttagcgaaa agttgaataa aaaatcggca
      361 tacaagtaca catcatcttt tttgtattgt aataagtgtt cgttatgcag cttacgcccc
      421 caccatgatt ggttaaactt tgttgacttt attgattttg tgttcaatga atcatatgga
      481 gcaaccgtta caatatcggc actgtataac ggtttaaatg aagtatgcac tgcactgcct
      541 acggcatcgg tataaggctc gtaccgctga attatatcct tagataataa aatatactcg
      601 tgttgcgcat atactttgcc cagtattaca ccgatactta aaacaaatac agaccaaagt
      661 ttacgcatac atttttttaa tgagacaaat aacatcattt aattgttgta tggtagtagg
      721 tacaccaaaa ctaaaacgca aggtggtttg cgctaaatca gctgttaacc ccatagcctg
      781 taatacatgc gatggctgtt gtaatgccga agtacatgcc gagccgttag ctacacctat
      841 ttgtggcaat gccttataaa gttgcgtagc tgttgtatct gtaaaagtaa cgctgctggt
      901 gttaggtaac ctgggtactt tgttgccatt aatttgtaca ggtaattgat gtaatgcatc
      961 ttcaaaataa tgtaacaaat gatttaagtg ttgcatttgt gccgttaggt tagtttgtgc
     1021 taatgctgct gcagcgccca taccaactat acccggaacg tttaatgtac cgggtcggat
     1081 atttttttcg tggccgccac caaacaatag tggtttaagg tgtactctcg gattttttct
     1141 gcggatgtaa agcgcaccac aacctttggg gccatacatt ttatgagccg agcaaactaa
     1201 tacatccact gcaagtgtat ttacatcaac cggtattttg ccaacagctt gtgtagcatc
     1261 cgttaccata agacagtggt atttgtgtac tatttctgct atttccttta cgggttgcag
     1321 cactccggtt tcgttattgg caagcatcac acaaacagca atggtgtttg gtgttattgc
     1381 ttgtgttagt gcattcaaat caagctgtcc gtgtgtgtta accggtacaa aggttatgtt
     1441 ggccccaata gtttctaaat aacggcaagt gtcgcctaca gctttatgtt cggttgtaac
     1501 agttactata tggttgccct ttgttttata ggcgtggtag ataccttgta gtgcaatatt
     1561 tactgcctcg gtggcgcccg atgtaaaaat aatttcatcg ggttgtgcac caataagggt
     1621 tgctacttgt tgccgtgcgt tttctgtggc ttgttttgca taccatcccg cctgatgtgt
     1681 tgcactggat gcattgccaa aattatcagt aaaaaagggc aacatagcct gcactactac
     1741 ttcgtttacg ggtgtagttg cattataatc gaggtaaata aaaggcatgc tataaatagt
     1801 tttttacaac aatgcaaagc aactatttag ttttattttt tagccaacca aagttgtgga
     1861 tttagctttt cgaacccttt ccagatttgc aaattaatca tgcccttgcc cgtttcagaa
     1921 tcgttgccgg ccacaccaat tttttggcgt gtggttagtt gttcgccttt acttactgca
     1981 acggtttcta agttggcata aacggttaag tactcgccat gccgcacaat aactgcatta
     2041 ttactgcccg gcaaacttat tactgctgtt acctgcccgg taaaaatggt gcgtacaccg
     2101 gcttggttat ttgtggttat atcaacgcca tcatttttaa ccactacacc ttttaaagtt
     2161 gggtgtgcat gtgcgccaaa tttttcggta atggtgcctt gttcaactgg ccagggtaat
     2221 gtgccacggt tgccgctaaa gttgttggat agtttttgtg cttcaggtgt taaggttatg
     2281 gcgatggcgt tttc
//
LOCUS       DAOMAA010000289         2293 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_43601_length_2293_cov_3.199732, whole genome shotgun sequence.
ACCESSION   DAOMAA010000289 DAOMAA010000000
VERSION     DAOMAA010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43601_length_2293_cov_3.199732"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            79..1341
                     /locus_tag="PLO59_04705"
     CDS             79..1341
                     /locus_tag="PLO59_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00437.1"
                     /translation="MFGSVVLDVTIGLVFIYLLYSLLATILNEIITTNIGMRARFLRK
                     GIERMLNDEYVEKYENALSVLDKVRMFYRNTKRSIRGFFLLETGNYKKSFAAKFYDHP
                     AIYYMAENKLHSKPSYMDAQNFSDVLLAILSKNQGNPANDMLIIRKALQANEFELNGK
                     TIHLDKETREELMSKLVMADFDFVKFKASLETWFDQTMERTSGWYKRKVQLILFFVGF
                     FIAAAFNVNTFKIVDVLSKDKDARSQLVALAMSTSKSDSLNQIITISKDTTTLTSDSI
                     LRQTYETLLSDQADIANILGSGWGFDKMRKKVKDADGNVSLKRFNTWDKIKYIAWRSF
                     NPINNLWGFLVTAMAISLGAPFWFDLLNKLKAIKGSGQIPANDVKTTEATLTVNPSKI
                     KSVQPFDADTDETDADSDTEPVTPTDKK"
     gene            1619..>2293
                     /locus_tag="PLO59_04710"
     CDS             1619..>2293
                     /locus_tag="PLO59_04710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HQV00438.1"
                     /translation="MNVSFVTPAFLYALFLIAIPILIHLFNFRRFKKVAFTNVRFLQQ
                     LKEETNAQSKLKHLLVLICRILAIVFLVLAFAQPFIPQKKQPVAGQQAISVFVDNSFS
                     MDAIGSQGTLLQQAQEKAIEVARAYTATDKFQLLTADFNPENQRMLSREEFIDQVQQI
                     KSSAPGKTLSQIRARQNEALLNAGISRQQSYIISDFQQNYFDFNLLKTDTAPVSLIAL
                     AAKTTNN"
BASE COUNT          792 a          408 c          420 g          673 t
ORIGIN      
        1 aagccgcatt ggatatttat gatgatagct atcataaaag ctggttaact taaaataaac
       61 ccttaatacc gcaacactat gtttggttca gtagtattag atgtaaccat tggtttggtt
      121 tttatatacc tgttatacag tttattagcc accattttaa atgaaataat tacaaccaac
      181 ataggcatgc gtgcccggtt tttacgcaaa ggcattgagc gtatgctcaa cgatgagtat
      241 gttgagaaat atgaaaatgc actttcggtt ttagataaag tgcgtatgtt ttacagaaat
      301 accaaacgca gtatccgtgg gtttttctta ctcgaaaccg gtaattacaa aaaatcattt
      361 gctgccaaat tttacgacca tccggcaata tattacatgg ccgaaaacaa attgcacagc
      421 aaaccatcct atatggatgc gcaaaatttt tcagatgtat tgctggctat tttaagcaaa
      481 aaccaaggca accctgcaaa tgacatgctt attatacgca aagcattgca ggcaaatgaa
      541 tttgaactta atggcaaaac catacatctg gataaagaaa cgcgcgaaga gttgatgagt
      601 aaattggtaa tggccgattt tgattttgtg aaatttaaag caagtttaga aacctggttc
      661 gaccaaacca tggagcgtac ctcaggttgg tacaaacgta aggtacaact gatattattt
      721 tttgtaggct tttttattgc agcagctttt aatgtaaata catttaagat agtggatgta
      781 ttatcgaaag ataaagatgc ccgtagccag ttggttgctt tagctatgag taccagtaaa
      841 tcagacagtt taaatcagat aattaccatt agtaaagaca ccacaacact tacaagcgat
      901 agtatattac gccaaacgta tgaaacgcta ttatccgacc aggcagatat agccaatatt
      961 ttaggctcgg gttggggatt tgataaaatg cgtaaaaaag taaaagatgc tgatggcaac
     1021 gtatcgttga aacgatttaa tacctgggat aaaattaaat acatagcatg gcgctcattt
     1081 aaccccataa ataacctttg gggctttttg gtaacagcaa tggcaatatc gttaggtgca
     1141 ccattttggt tcgatttatt aaacaaactg aaagctataa aaggttcggg ccaaatacca
     1201 gccaacgatg taaaaacaac ggaagcaaca ctaacagtta acccatcaaa aattaaatcg
     1261 gttcaacctt ttgatgcgga tacagatgaa actgatgcgg atagcgatac cgagccagtt
     1321 acaccaactg ataaaaaata gatttgaatt tagttattga ttcaagcgca cggttcctaa
     1381 attaaaatgc aggccttttg tttgcttttg atatttacgt tatcagcgta tcagatttta
     1441 atctttagaa ttatctggaa aataggaagt aaaacgggtt ttgtttttta aaagaataat
     1501 tttcagttaa aaaatgagct tactaataaa tgaagccctg acaacgtttt aatgcaactg
     1561 ctttggttta gccatattta tatattcgcc acgccataaa aagcatttcc attaaataat
     1621 gaatgtaagc tttgtaaccc cagcattttt atatgcgctt tttctgatag ccatacccat
     1681 attaatacat ctttttaatt tcagacgatt taaaaaagtt gcatttacca atgttagatt
     1741 tttacaacaa cttaaagagg aaaccaatgc acaaagtaag ctaaagcact tgttggtact
     1801 tatatgccgt atattggcta tagtgttttt agtgttggca tttgcacaac cattcattcc
     1861 acaaaaaaaa caaccggtag caggccagca agccataagt gtttttgtag ataattcatt
     1921 tagcatggat gctattggca gtcagggtac gttgctgcaa caggcacaag aaaaggcaat
     1981 agaagtagca cgtgcttata cagctaccga caagtttcag ttgcttacgg ctgattttaa
     2041 ccccgaaaat caacggatgc ttagccgcga agaatttatt gatcaggtac aacaaattaa
     2101 aagcagtgca ccgggtaaaa cactatcaca aataagagcc aggcaaaacg aggcattact
     2161 aaatgcaggc atcagccggc aacagtcata catcatttcc gattttcaac aaaactattt
     2221 tgattttaac ttgcttaaaa cagatacggc acctgtatcg ttaattgcac ttgctgcaaa
     2281 aaccacaaat aat
//
LOCUS       DAOMAA010000290         2292 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_43655_length_2292_cov_2.448815, whole genome shotgun sequence.
ACCESSION   DAOMAA010000290 DAOMAA010000000
VERSION     DAOMAA010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43655_length_2292_cov_2.448815"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /gene="miaA"
                     /locus_tag="PLO59_04715"
     CDS             complement(<1..720)
                     /gene="miaA"
                     /locus_tag="PLO59_04715"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HQV00439.1"
                     /translation="MQRKLIVVGGPTGIGKTDVSIILALALDAEIISFDSRQFFIETQ
                     IGTAKPTIDQLKKVKHHFINSHHVWQPFTAGAFAQTCNKLISENPSQNFVLVGGSGMY
                     IDAWLHKLDDLPLMDATYRKQLDVWYENNGIAFLQAMLKQADPTYYYTVDLFNKQRVM
                     RALEVCHLGNKPYSAYLAGKEKNVMEVIKLGLNMPRQQLYNRINCRVDSMIAAGLVNE
                     CKLLLPYKEMQALKTVGYKEMF"
     gene            complement(752..>954)
                     /locus_tag="PLO59_04720"
     CDS             complement(752..>954)
                     /locus_tag="PLO59_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HQV00440.1"
                     /translation="ETGLGKYDFEQFAKALRYGNSPHGPLRYPMFPYTILDDLETKAI
                     WTYLNSVPKIKNDVSKKYTSSK"
     assembly_gap    955..964
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<965..1711)
                     /locus_tag="PLO59_04725"
     CDS             complement(<965..1711)
                     /locus_tag="PLO59_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HQV00441.1"
                     /translation="MKKFFKIILAVVLVVVLLLAIGYCFIAIKGIPSYTAQTVTVKVS
                     HTPQNVERGLRLASVLCIKCHTGNNSNQLTGREMFDMPPEFGKVYSSNITQHQQYGIG
                     KWTDAQLVYFLRTGVKPDGTYAPIWMPKFVKMSDEDLFSIIAYLRSNHPSVQPAEAVT
                     QKCEPSWLAKFLCNVAFKPLPYPEAPVPEPDTNNLLAYGRYLVAGRFDCFPCHSADFK
                     TVNVFEPEKSEGYLGGGNLLLNEAGAPVYSA"
     gene            1852..>2292
                     /locus_tag="PLO59_04730"
     CDS             1852..>2292
                     /locus_tag="PLO59_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HQV00442.1"
                     /translation="MLLSATTENYLKAIYKLNAQTTIVTTNNIALALGTTAPSATDMI
                     KKLALNKLITHTKYRGVGLTAKGNKAALTIIRRHRLWELFLNKVLGFKWDEVHQMAEQ
                     LEHVSVPELIERIDQYLGYPKFDPHGDPIPDAMGKIAKHQYQSLL"
BASE COUNT          739 a          453 c          403 g          687 t
ORIGIN      
        1 aaacatttct ttatagccta ctgtttttaa agcctgcatc tctttgtatg gtaataacaa
       61 cttacattcg ttaaccagac cggcagcaat catgctatca actctgcaat ttatacggtt
      121 gtacaactgt tgacgcggca tgtttaaccc aagtttaatc acctccatta catttttttc
      181 ttttcctgct aaataggctg agtatggttt attgcccaaa tggcaaactt ccaaagcacg
      241 cattactctt tgtttgttaa acaaatcaac cgtgtaataa tatgtcggat cggcttgttt
      301 cagcattgcc tgtaaaaaag caatgccatt gttttcatac catacatcca attgctttct
      361 ataggttgca tccatcaaag gcaaatcatc cagtttgtgc aaccaggcat caatatacat
      421 acccgaacct ccaaccagta caaagttttg actggggttt tctgaaatta atttgttgca
      481 agtttgggca aatgcacctg cagtaaaagg ctgccacaca tgatggctgt ttataaaatg
      541 atgtttaact ttttttaatt gatcgatagt tggcttagct gtgccaattt gcgtttcgat
      601 aaaaaactga cgcgaatcaa atgaaataat ttcggcatcg agcgctaagg ccaatataat
      661 actcacatcc gttttaccaa taccggtagg gcctccaaca acaattaact tacgctgcat
      721 aacctataca ccaacagtta caaaaattta attatttaga tgaagtatat tttttgctta
      781 catcgttttt aatttttgga accgaattta aataagtcca aatggctttg gtttctaaat
      841 catccaaaat ggtataagga aacattgggt aacgtagtgg gccatgtggt gagttgccat
      901 aacgcaaagc cttagcaaat tgttcaaaat cgtacttacc taaaccggtt tcggnnnnnn
      961 nnnnagctga ataaacgggt gcaccggctt cattaagcaa taaattacct ccgcccaaat
     1021 agccctccga tttctcaggt tcaaaaacat ttacagtttt aaaatcggca ctgtggcatg
     1081 gaaaacaatc gaaacggcct gcaactaaat aacggccata agccaataaa ttattggtat
     1141 cgggttcggg tacaggtgcc tcgggatagg gcagtggttt aaaggcaacg ttgcacaaaa
     1201 attttgcaag ccaactgggc tcacatttct gcgtaaccgc ttcggccggt tgaactgacg
     1261 ggtggttgct tcgtaaataa gctataatcg aaaacaaatc ttcatcgctc atttttacaa
     1321 atttaggcat ccaaataggt gcatacgtac catcaggttt tacaccggta cgtaaaaaat
     1381 aaaccaattg cgcatcagtc catttgccaa taccatattg ctgatgctgc gtgatgtttg
     1441 acgaatatac tttgccgaat tcaggcggca tatcaaacat ttcgcgcccg gttaactgat
     1501 tactgttgtt acctgtatgg cattttatac aaagcactga tgccaggcgt aagccccgct
     1561 ctacattttg tggtgtatgt gatactttaa ccgtaactgt ttgtgctgtg taacttggta
     1621 tgcccttaat agcaataaag caatagccaa tagctaacag taatactaca acaagtacta
     1681 ctgctaaaat aattttaaaa aattttttca tgggatgttt agttttggtg tggtatgcaa
     1741 ttctaataaa aataaattaa attttgttaa aaataactac gtgcaagaaa aatgttttag
     1801 attagtctaa atttattttt ctataaatca aaatatttta aacgactaca catgctattg
     1861 agcgcaacca ccgaaaatta tttaaaagct atttataaac ttaatgcgca aacaacaatt
     1921 gttaccacca acaatatagc actggcatta ggtactacgg caccttcggc tactgatatg
     1981 attaaaaaac ttgcgctaaa caaacttata acacatacca aataccgtgg tgtaggctta
     2041 acagccaagg gcaataaagc tgcattaacc atcatacgca ggcatagatt atgggaattg
     2101 tttttaaata aagtattagg ttttaaatgg gatgaagtcc atcagatggc cgaacagtta
     2161 gaacatgttt cagtaccgga actaatcgaa agaatagacc aatatttagg ttatcctaaa
     2221 tttgacccac atggcgatcc cataccggat gcaatgggca aaattgcaaa acatcaatac
     2281 caatcattat ta
//
LOCUS       DAOMAA010000291         2290 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_43717_length_2290_cov_2.906040, whole genome shotgun sequence.
ACCESSION   DAOMAA010000291 DAOMAA010000000
VERSION     DAOMAA010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43717_length_2290_cov_2.906040"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="PLO59_04735"
     CDS             <1..610
                     /locus_tag="PLO59_04735"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HQV00443.1"
                     /translation="DIIAGGAGKAVFMGTYGSSSSLFYNPAAAAFKNQFDASAGWPAF
                     CVNGRNRTAYSSSGGIYTSTTKTNVLAAIDSTAAATAKVNTGFKMTYGTDSVRVSTRT
                     KFFAEMSGDIYLGVYLIEDGALGVQTGLSGTVSHHHVLQGSFSQPFGTQIATGTTAAN
                     KEVLSSFALKTNAAWDKTKLKIATVIWKKNGSTYEFVNAYTE"
     gene            complement(682..1128)
                     /locus_tag="PLO59_04740"
     CDS             complement(682..1128)
                     /locus_tag="PLO59_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1572 family protein"
                     /protein_id="HQV00444.1"
                     /translation="MVKQTIVEFLERDLNTLKLEIEKYKSDDDLWKVADGISNCAGNL
                     CLHLCGNLKHYIGALLGHTGYIRHRDAEFDLKDIPAHALIADIDITKNIVTETLQKLS
                     DADLLKTFPDLKHGKSVTALHMLLHLMLHFQYHLGQINYHRRILGS"
     gene            1312..>2290
                     /gene="ligA"
                     /locus_tag="PLO59_04745"
     CDS             1312..>2290
                     /gene="ligA"
                     /locus_tag="PLO59_04745"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HQV00445.1"
                     /translation="MNLETAKLAVIELTEQINAHNYAYYVLAQPTISDVEFDMLLQKL
                     VSIETQYPQLVQAHSPSQRVGSDVVKSFNQVKHQYPMLSLGNTYSQADLHDFDERTRK
                     LIGNNFEYVCELKFDGVAIGLTYVNGILQQAVTRGDGVQGDDVTANVKTIKSIPLQLH
                     GNNYPSNFEIRGEIFLPRAEFDRLNQELILQLTDEGYNVDEINERLYRNPRNTASGTI
                     KQQDSKLVAQRKLDCYLYNIFGDDLPFKNHYDALEKARSWGFKISEHSKLCKSIEAVF
                     DFINHWDVKREQLPFDIDGIVLKVNDYAQQEELGYTAKSPRWAISYKFKA"
BASE COUNT          771 a          423 c          413 g          683 t
ORIGIN      
        1 agatattatt gccggtggtg ccggcaaagc tgtttttatg ggtacttacg gaagcagcag
       61 cagtttgttt tataaccctg cagccgctgc ttttaaaaat cagttcgatg caagtgcagg
      121 ctggccggca ttttgtgtaa atggtcgtaa tcgtacggca tacagttcat caggtggtat
      181 ttatacatca actactaaaa ccaatgtatt agctgcaatt gactctactg ctgctgctac
      241 ggctaaagtt aataccggct ttaaaatgac ttatggtacc gatagtgtta gagtaagcac
      301 acgtaccaag ttttttgccg aaatgagtgg tgatatctat ttaggtgttt acttaatcga
      361 agatggcgca ttaggtgtac aaaccggcct tagtggtacc gtatctcacc atcatgtttt
      421 acaaggaagt ttttcacaac catttggtac acaaatagcc accggtacca ctgcggctaa
      481 taaagaagtt ttgtcaagct ttgctttaaa aacaaatgca gcctgggata aaaccaaatt
      541 aaaaattgca accgttattt ggaaaaaaaa tggaagcact tatgaatttg taaatgccta
      601 tactgaataa atttttacag tttcaataaa aaaaggctgc ctcattattt taaggcagcc
      661 tttttttaca atcaaaaatt cttaagatcc tagaatacgt ctgtgataat ttatctgacc
      721 caaatgatat tgaaagtgca acatcaaatg cagcaacatg tgcaaggcag ttacagattt
      781 accatgtttt aaatcaggga acgtttttag caagtcggca tcagaaagtt tttgcagcgt
      841 ttcggttaca atattttttg taatgtcaat atcggcaata agtgcatgag caggtatgtc
      901 ctttaaatca aattcagcat ctctatgcct gatataacca gtgtggccta acaatgcacc
      961 aatataatgt ttaaggttac cgcataaatg caagcaaagg ttgcctgcac agttcgaaat
     1021 tccatcagca actttccata aatcatcatc cgatttatac ttttcaattt caagttttag
     1081 cgtatttaaa tcacgctcca aaaattcaac tattgtttgc tttaccataa ctaatatatt
     1141 taaaagtgga tataaaataa aaactgtttt ctacttcaaa tttaaaaagt tacctgatta
     1201 aacactattt aaatcccatt tatttttcgt atttgaaaaa taaattgaaa cagcgcatta
     1261 tgtaacattt aaaataaatt catttgcatt tcaacaccaa aaaaaaggtc catgaattta
     1321 gagacagcaa aacttgccgt aattgagcta acggaacaaa taaatgcaca caactatgcc
     1381 tattatgttt tggcacaacc aaccattagc gatgttgagt ttgatatgct gttacaaaaa
     1441 ttagtttcaa tagaaacgca atacccacag cttgtacaag cccactcccc ttcgcaacgt
     1501 gttggcagcg atgtagtaaa atcatttaat caggtaaaac atcaataccc catgttatcg
     1561 ttaggtaaca cttactcaca agccgacttg cacgactttg atgaacgaac tcgtaaatta
     1621 attggtaaca atttcgaata cgtttgcgaa ttaaagtttg atggtgtagc aattggccta
     1681 acttacgtaa atggcatatt gcaacaagca gtaacacgtg gcgatggtgt gcagggagat
     1741 gatgttaccg ccaatgtaaa aacaataaag agtattcctt tgcaattaca tggcaataac
     1801 tatccatcta acttcgaaat aagaggcgaa atattcttgc cccgggctga atttgaccgc
     1861 ttaaaccagg agcttatact gcaacttaca gacgaaggat ataacgttga tgagataaac
     1921 gaaagactct atcgcaatcc acgtaataca gcctcaggca ccattaaaca gcaagattcg
     1981 aagttggttg cccaacgtaa actcgactgt tacctatata atatttttgg tgatgattta
     2041 ccatttaaga atcattacga tgcgctcgaa aaggccagaa gttggggatt taaaatttca
     2101 gaacattcaa aattatgtaa atcaattgaa gccgttttcg actttataaa ccactgggat
     2161 gtaaagcgcg agcaactacc ctttgatatt gatggtattg tattaaaagt aaatgattat
     2221 gcacaacagg aagaattggg ttatacggca aagtcgccac gttgggcaat atcctataaa
     2281 tttaaagctg
//
LOCUS       DAOMAA010000292         2289 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_43752_length_2289_cov_2.469114, whole genome shotgun sequence.
ACCESSION   DAOMAA010000292 DAOMAA010000000
VERSION     DAOMAA010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43752_length_2289_cov_2.469114"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="PLO59_04750"
     CDS             complement(<1..427)
                     /locus_tag="PLO59_04750"
                     /inference="COORDINATES: protein motif:HMM:NF012355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HQV00446.1"
                     /translation="MKKIYLSTLLFLFTAIVFATTHTIQVANFNFSPSVVNATVGDTI
                     HFEWVIGSHTTTCDGSAGSILPNGAMPWNANMNSNNQTFDYVLTTAGTYTYVCLPHSS
                     FMIGMLNVQPAAVTSEVFFRVDMSNETLGLGGVHVAGSFN"
     gene            complement(647..>2289)
                     /locus_tag="PLO59_04755"
     CDS             complement(647..>2289)
                     /locus_tag="PLO59_04755"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQV00447.1"
                     /translation="SFFAVDYLPANIRFIYGADSGGNENAHLYLHNRTDAAAKDLTPW
                     PNSKNNFWGWSKTKTHMYVLSNKRNPKYYDVWKLDTATWQPVLLFQNDSGFEVSLMSH
                     NEQYIALNKSISTDKNELYLFDRNTQQFKRLSNDNEATWTANAFENNDTIMYYTTSDG
                     AEFKYLVKYYLRTGKTEKIYQCNWDVTTISLSENEQYRTVFINEDGKNKVLLFEHASN
                     KLIQFPEIKDSDILNIKISDSEQNMLLTVGSSRSPQNLFVYNFKNKKLKQLTRTLNNE
                     VDVNDLVKAKIVRYKSFDGTEIPAIYYKPLQANIQSKVGALVWVHGGPGGQSRVGFSN
                     AIQYLVNHGYAVLAVNNRGSSGYGKTFYKMDNKDQSNGDLKDCIYGKKWLASQNYIDT
                     NAIGIYGGSYGGCMVLAALAFYPDAFNVGVNLFGVANWIRTLRSIPPYWEARRKALYQ
                     EIGDPNTTDSVRLKNISPIYNYQKITKPLLVFQGANDVRVLPIESDEIVAGVKKNNVP
                     VEYVVYPDEGHGFVKKENQITTAKRTLAFLNKYLKPNK"
BASE COUNT          651 a          433 c          415 g          790 t
ORIGIN      
        1 cattaaaact tccggccacg tgaacgccac caagacctaa agtttcatta ctcatatcta
       61 ctctaaaaaa cacttcgctg gtaactgctg ccggttgtac gtttagcatc ccaatcataa
      121 aactactgtg tggtaaacac acataagtat aggtgcctgc agtggtaagt acataatcga
      181 atgtttgatt gttgctgttc atgttggcat tccagggcat tgctccattt ggcaatatgc
      241 tgcctgccga accatcgcaa gttgtggtat gcgaaccaat tacccattcg aaatggatgg
      301 tatcgcctac tgttgcatta acaactgaag ggctaaagtt gaaatttgct acctgtatgg
      361 tatgtgtagt agcaaagact attgctgtaa acagaaacaa tagcgttgat aagtaaattt
      421 ttttcatttc atataagttt aattagttaa taataagttt agctaagtac gtatttttaa
      481 tacaacactt gcaatatgct aatagcacat gtattgataa aattaaaaac ttaagcaatg
      541 ttttcgaatg taggtttgca gccacatttg ttttattcat gtaagtaata acagggttct
      601 tgttgttatt tgatggtaaa agcccgtgtt gattcgatca actaaattac ttgttgggtt
      661 tgaggtattt gtttaaaaac gcaagcgtac gttttgcagt agttatttga ttttcctttt
      721 taacaaagcc atgtccttcg tcaggataaa ctacatattc aacaggtaca ttgttttttt
      781 taacacctgc aacaatttca tcgctttcga taggtaatac acgcacatcg tttgcacctt
      841 gaaacaccag taatggttta gtgatttttt gatagttgta aatgggtgaa atgtttttaa
      901 gtctcacact atcggtagta tttggatcgc caatttcctg atataatgct ttacgcctcg
      961 cctcccaata aggcggaatg ctgcgtaatg tgcgtatcca gtttgctacg ccaaacaaat
     1021 taactccgac attaaatgca tcaggataaa aagcaagtgc ggctaaaacc atacaaccgc
     1081 cataactgcc accgtatata cctatggcat tggtatcaat ataattttgt gatgccagcc
     1141 attttttacc ataaatacaa tcttttaaat cgccattact ttggtctttg ttatccattt
     1201 tgtaaaacgt ttttccatag ccactactcc ctctgttgtt aacagcaagt acagcatatc
     1261 cgtggttaac taagtattga atagcgttgc tgaaacctac ccgactttga ccacccgggc
     1321 caccatgtac ccaaaccaaa gcgcccactt tactttgtat gttagcttgt aatggtttgt
     1381 aataaatggc cggtatttca gtaccgtcaa acgatttata tctcacaatt ttagctttta
     1441 ccaaatcgtt tacatccacc tcgttattaa gtgtgcgggt gagttgcttt aattttttat
     1501 tcttaaaatt ataaacaaat aggttttgtg ggctacggct actgccaaca gttagcagca
     1561 tgttttgttc tgaatctgat attttaatat ttaagatatc gctatctttt atttcaggaa
     1621 attggatgag tttattgctt gcatgttcaa aaagcaaaac tttgtttttg ccatcttcat
     1681 taataaatac agttcggtac tgctcatttt cgcttaggct tatggttgtt acatcccaat
     1741 tacattggta aattttttcg gttttgccgg tgcgtaaata atactttacc aaatacttaa
     1801 actctgcacc gtcagaggtg gtgtaataca ttatggtatc gttgttttcg aaagcgttgg
     1861 cagtccaggt agcctcattg tcattactta atcgcttaaa ttgttgtgta tttctatcaa
     1921 acagatagag ttcgttttta tcggtactga tacttttatt taaggctatg tattgttcgt
     1981 tatggctcat taacgacacc tcaaatccgg agtcgttttg aaaaagcaat accggctgcc
     2041 aggttgctgt atctaatttc catacgtcat aatatttagg gttgcgttta ttgcttaaaa
     2101 catacatgtg cgtttttgtt ttgctccaac cccaaaaatt gtttttgcta ttgggccagg
     2161 gcgttaaatc tttggcagcg gcatcggttc tgttatgtaa atataaatgt gcgttttcgt
     2221 taccgcccga gtcggcacca tatataaaac gaatgtttgc cggcaaataa tcaacagcaa
     2281 aaaacgatt
//
LOCUS       DAOMAA010000293         2285 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_43872_length_2285_cov_3.178924, whole genome shotgun sequence.
ACCESSION   DAOMAA010000293 DAOMAA010000000
VERSION     DAOMAA010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43872_length_2285_cov_3.178924"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            10..789
                     /locus_tag="PLO59_04760"
     CDS             10..789
                     /locus_tag="PLO59_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HQV00448.1"
                     /translation="MNVPTLASMLAEGKTPEYLFWVGCAGSFDQRAQKVTKALVKILN
                     HTGVSFAVLGTEESCTGDPAKRAGNEFLFQMQALMNIQLLQGYEVKKIVTACPHCFNT
                     IKNEYPDLGGTFEVIHHSMLLQQLINSGKLTINGGTFKGKKVVFHDPCYLGRANDVYE
                     APREVIQKLDVALSEMHRSRAKGLCCGAGGAQMFKEPEPGSKDVNVERAEEALELNPN
                     VIAVACPFCMTMLTDGVKNFNKEDAVQVLDIAELIAQAEEL"
     gene            complement(913..>2285)
                     /locus_tag="PLO59_04765"
     CDS             complement(913..>2285)
                     /locus_tag="PLO59_04765"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV00449.1"
                     /translation="TPVSSQESSDGAAGFYQYIPEGFQIIKNTGAAVALDQSIDVATD
                     YTDNMILHKGTWGAGAYTYTRSLVPVPVSTSAAGSKAMATYTIAMSDDGMVGYIVAIG
                     HNDFTLFPDEAYYPIVYKTTDGGTTWVGPWNLDMAPLEAVIPAYDGTTGNLVSTSFEV
                     DAVMDNANNLHIITSACAGAGTFSIYQGDSVSAVVDIYTTDGGASWKAQELGLPVTAR
                     GTYGDNSATNANLTHDNRTQASRNWDGTKLMFTWFDTDEASFPGLGNTYPDAYAIAYN
                     VTTGLWTAAANKTPGLSTIMFGQCSYYAMDAAGTQTVPIAFQVISGTTATGEPLNTGG
                     VVDYVYTGDVTFNDADYTVVGNPINIPIAAGVNEQPIVSSSVSLVPNPAAGSTKLTYL
                     LNTSSDVTIKVVNMIGATVVEMVKPAQTAGQHEVALNVSELKAGVYFVNVTAKGSVST
                     TKLIVK"
BASE COUNT          738 a          436 c          447 g          664 t
ORIGIN      
        1 aatactttta tgaatgtacc aaccttagca tctatgctgg ccgaaggtaa aacacccgaa
       61 tatttatttt gggttggatg tgccggtagt tttgatcagc gcgcacaaaa agtaaccaag
      121 gcattagtta aaatattaaa tcataccggt gtttcatttg ctgttttagg tacagaagaa
      181 tcttgcacag gcgatccggc aaaacgagcc ggtaatgaat ttttgtttca aatgcaggca
      241 ttaatgaata ttcagctatt acaaggttat gaagtaaaaa aaatagttac tgcttgtcca
      301 cattgtttta atactataaa aaatgaatac ccggatttgg gcggtacatt cgaagttatt
      361 catcacagta tgttgttaca gcaacttatt aactcaggca aactaactat caacggaggt
      421 acatttaaag gtaaaaaagt tgtgtttcac gacccatgtt atttagggcg ggcaaacgat
      481 gtatatgaag cgccacgtga agttattcaa aagttagatg tggcattaag tgaaatgcac
      541 cgaagccgcg caaaaggctt atgttgtggc gcaggaggtg cacaaatgtt taaagaaccc
      601 gagcccggaa gtaaagatgt aaatgtggaa cgtgccgaag aggcacttga attaaatccc
      661 aatgtaattg ctgtagcctg cccattttgt atgactatgc ttaccgatgg cgttaaaaat
      721 tttaataaag aagatgcagt tcaggtttta gatattgctg agttaattgc acaagccgaa
      781 gagttgtaac gatttaccgc taaattttat atccaataaa aaagcctcag atcgaaatat
      841 catctgaggc ttttttatta agcaaaaaaa atcccgatta acaaatcggg atttttttaa
      901 aagtgcgtaa tattacttaa caataagttt agtagtactt acacttcctt tagctgtaac
      961 gtttacaaag taaacaccgg ctttaagttc agatacgttt aaagcaactt catgttgacc
     1021 tgcagtttga gctggcttaa ccatttcaac aactgtagca ccaatcatgt taaccacttt
     1081 aatagttaca tcacttgaag tgtttaataa gtaagtaagt tttgtgcttc cggcagctgg
     1141 gtttggaacc aatgaaactg aagaagatac aattggttgt tcgttaacac cggcagcgat
     1201 cggaatgtta attgggttac caacaacagt gtaatcagca tcattaaatg ttacatcgcc
     1261 cgtataaaca taatcaacaa caccacctgt atttaagggt tcgccagttg cagttgttcc
     1321 actaataacc tggaaagcaa tcggtacagt ctgtgtaccg gcagcatcca ttgcataata
     1381 tgaacattgg ccaaacataa tagtacttaa accaggcgtt ttatttgcag cagcagtcca
     1441 taaaccggtt gttacattgt aagcaattgc ataagcatct gggtaagtat tacctaagcc
     1501 tgggaatgaa gcctcatcag tatcaaacca ggtaaacatt aatttagtac catcccagtt
     1561 acgtgaagct tgtgtacggt tatcatgggt aaggtttgca ttagtagcac tgttatcacc
     1621 gtaagtacca cgggcagtaa caggtaaacc tagttcttga gctttccagc ttgcaccacc
     1681 atcagtagta taaatatcaa caactgcaga tacgctatca ccttgataaa tagagaatgt
     1741 acctgcacct gcacatgctg atgttataat gtgtaagtta tttgcattat ccataactgc
     1801 atcaacttca aatgaagtag ataccagatt acctgtagta ccatcataag caggtataac
     1861 tgcttcaagt ggagccatat ctaaattcca aggaccaacc caagtagtac caccatcagt
     1921 tgttttataa acaattggat aataagcttc atcagggaat aatgtaaaat cattatgtcc
     1981 gatagcaact atatatccta ccataccgtc atcgctcatt gcaattgtat aagtagccat
     2041 tgctttacta ccggctgcac ttgtagatac cggaaccgga actaaactgc gggtataagt
     2101 atatgcacca gcaccccagg tacctttgtg tagtatcata ttgtcagtgt aatcagttgc
     2161 aacatcgatt gattgatcta atgcaacggc agcacctgtg ttttttataa tttgaaaacc
     2221 ttctggaatg tactgataaa aacctgcagc accgtcagaa ctttcttgtg acgacacggg
     2281 tgtgg
//
LOCUS       DAOMAA010000294         2285 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_43876_length_2285_cov_3.029148, whole genome shotgun sequence.
ACCESSION   DAOMAA010000294 DAOMAA010000000
VERSION     DAOMAA010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43876_length_2285_cov_3.029148"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..1120)
                     /locus_tag="PLO59_04770"
     CDS             complement(11..1120)
                     /locus_tag="PLO59_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009126700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HQV00450.1"
                     /translation="MENKIPLMVPDIKPDDIKAVTDVLQTGMLIQGKQVALFEQDVAR
                     YLNVKHAIAISNGTATLHLALIAAGIGQGHEVIVPALSYVATANVVELVGATPVFVDI
                     DLQTFNIDVSKIESAITPKTKAIIPVHEFGLLANMELLMPIAIKHNLIVIEDAACALA
                     ASVGCQFAGTFGTLGSFSFHPRKAITSGEGGMLITNDDALEKKLRVLRNHGIQMEDGK
                     MQFVAAGFNYRLTDFQAALVHSQFKRLPQILAYKQTLAQAYLCALQHCKNIMLPIIPN
                     GYYHTWQSFHIIIEGIQRDEVIMRLQDVGIGTNYGAQCIPQQKYYQYKYKLNTATLFP
                     NALKAYQQGLVLPLYEKLSVADIQHVATALLKIVG"
     gene            complement(1120..2073)
                     /locus_tag="PLO59_04775"
     CDS             complement(1120..2073)
                     /locus_tag="PLO59_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQV00451.1"
                     /translation="MIKERKIFITGGAGFIANTLIKNLIDDNQITVYDNFHRDTLTAS
                     GYIQHPNIKIIKGDVIDKAHLTASMQGAQIVVHAAGIAGIDTVIKDPVRTMTVNMIGT
                     ANALEAAYINKVSHRFIDFSTSEIFGSMAYKSSEADNTISGSAGEARWVYAVSKLAGE
                     HLANAYHKQYGLPTVTVRPFNVYGPGQTGEGALQIFIKRALNNEDIYINGDGTQIRAW
                     CYVDDFVDCLMRCISDEKAIGESFNLGNARAVITTLGLAQTVCRVLASKSKILFRDQL
                     SADIELRIPSVTKTENVLGFKAKVDLEEGILKTADYFKQLV"
BASE COUNT          689 a          462 c          399 g          735 t
ORIGIN      
        1 actataaaaa ttacccaaca atttttagta aggcagtagc tacatgctgt atatctgcaa
       61 cacttagttt ttcgtaaagg ggtaaaacca aaccttgttg gtaagccttt agtgcatttg
      121 ggaaaagcgt agctgtattt aatttgtatt tgtactgata atatttttgc tgtggtatgc
      181 attgggcgcc atagttagta ccaataccca catcttgtaa acgcataatt acttcatcgc
      241 gttgtatgcc ttcgattata atgtgaaacg attgccaggt gtgataataa ccattgggta
      301 ttatgggcaa cattatgttt ttgcaatgtt gtaatgcaca gagataagct tgggctaatg
      361 tttgtttata ggctaatatt tgtggtaaac gtttaaactg actgtgcact aacgctgcct
      421 gaaaatcggt tagacggtaa ttaaaaccgg ctgccacaaa ctgcatttta ccatcctcca
      481 tttgaatacc atgattacgc aacaccctaa gttttttttc caaagcatcg tcattggtaa
      541 ttaacatacc accctcgcca ctggtaattg ctttgcgtgg atgaaatgaa aacgaaccca
      601 aagtgccaaa tgtacccgca aattggcagc ctacactggc tgctaatgcg catgccgcat
      661 cttcaattac tatcaggtta tgttttatgg caatgggcat aagcaattcc atattggcta
      721 acaaaccaaa ttcatgcacg ggaataatgg ccttggtttt tggtgtaatg gccgattcaa
      781 ttttacttac atcaatatta aatgtttgca aatcaatatc aacaaaaacg ggtgttgcac
      841 caaccaactc cacaacattt gccgtagcta catacgacaa agcaggtaca ataacttcgt
      901 gcccctggcc aataccggca gcaattaacg ctaaatgcaa tgttgccgta ccattactta
      961 ttgcaatggc atgttttacg tttaaataac gtgctacatc ttgttcgaaa agcgctactt
     1021 gtttaccttg aatgagcata cctgtttgta gcacatctgt taccgctttg atatcatcag
     1081 gcttaatatc gggcaccatt aaaggaattt tattttccat taaacaagct gtttaaaata
     1141 atccgctgtt tttaatatac cttcttctaa atcaactttg gccttaaagc ctaatacatt
     1201 ttcggttttt gttactgatg gtattctcaa ttcgatatcg gctgaaagtt gatcgcgaaa
     1261 taaaattttt gattttgatg ccaataccct acaaactgtt tgggctaaac ccagtgtggt
     1321 aataacagca cgtgcattac ccagattaaa actctcgcca attgcttttt catcactaat
     1381 acagcgcatc aaacaatcca caaagtcatc tacataacac caagcacgta tttgtgttcc
     1441 atcgccatta atataaatgt cttcattatt taatgcgcgt ttaataaaaa tttgcaatgc
     1501 accttcgcct gtttggcccg ggccatatac attaaatggc cgcacagtta cggtgggcaa
     1561 accgtattgt ttatgatagg catttgccag atgctcgcca gcaagcttac ttactgcata
     1621 tacccagcgt gcttcgcctg ccgaacctga aatggtgttg tcggcttcgg aagatttata
     1681 ggccatactg ccaaatattt cagaggtcga aaaatcaata aaacggtgtg ataccttatt
     1741 aatataagca gcttctaagg cattggctgt gccaatcata tttactgtca tggtgcgcac
     1801 cggatcttta ataaccgtat ctataccggc aataccggca gcatgtacaa caatttgtgc
     1861 gccttgcatg gaagcagtta aatgtgcttt atcaataaca tcacccttaa ttattttaat
     1921 attgggatgt tgtatgtagc cacttgcagt taatgtatcg cgatgaaaat tatcataaac
     1981 cgttatttga ttatcgtcta tcagattttt aataagtgta ttggcaataa acccggcacc
     2041 accggtaata aatatttttc tttcttttat cataattagt acacgcttaa cctacacatt
     2101 gccttggcag tattgcacaa agcatgatgc aacattacac gaaataatta taataatcaa
     2161 tgtagccaaa tgctttaata gcgccacaat tgtttgtata aacaattttt aattatcggt
     2221 tatctaaaat tgatttagat tattgatttg catttatagt tttacacact gtgctttata
     2281 accaa
//
LOCUS       DAOMAA010000295         2284 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_43916_length_2284_cov_2.558098, whole genome shotgun sequence.
ACCESSION   DAOMAA010000295 DAOMAA010000000
VERSION     DAOMAA010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43916_length_2284_cov_2.558098"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /locus_tag="PLO59_04780"
     CDS             complement(<1..879)
                     /locus_tag="PLO59_04780"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HQV00452.1"
                     /translation="MAVKKKSKRTKNKVVHHTPPGTSPGTIVVADDASKPKLILYGYN
                     TEVFETIADISINTAVTLIKANPAHNYWLQIKGIGDKQLLETISSAFGIHPLEMEDVT
                     KLYQRPKFEEHQSHLFIIARLLGRSEEGLLQNDQLSVFCGNNFVITLQANYDSVFEVI
                     VNRLKAGRGMMRTAGSGYLTYAINDAIVDSYFPLLEKMGDALDELEDELLTKPTKLSM
                     EKIQIIKRDLILFRRAAFAERDKVNDILRSHNDFINVSVSPYLKDTYDHIIQVLDMVD
                     SYKEITASLMDIYVSSM"
     gene            1015..1740
                     /locus_tag="PLO59_04785"
     CDS             1015..1740
                     /locus_tag="PLO59_04785"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQV00453.1"
                     /translation="MVNWYNKWFGTRYYDLLYNKRDDLEAKHFLDTLVKQLGLEPQAQ
                     ILDVACGKGRHAIYLNKLGFNVTGIDLSDNSIAHSKQFENPSLHFYVHDMRRLFRTNS
                     FDLLLNLFTSFGYFERQHHNAMAINAWAAALKSGGKLVIDFFNVQKIAMQLVPHQTKQ
                     IDGIGFNISKSFENGYITKTIKVNDDNIEKYFYERVQALTLNQFEIYFKNAGLKIDSI
                     FGDYDLNSFVPTDSDRLIIIAKK"
     gene            1740..>2284
                     /locus_tag="PLO59_04790"
     CDS             1740..>2284
                     /locus_tag="PLO59_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00454.1"
                     /translation="MLLHGEVATQVAAHIGWMGYSALFLVVLISGLGFLFFKEIKPAN
                     LKLMLSFTGSFLFALCVMHMIPEVYEADSHIGLYILIGFFSQLLLEFLSEGIEHGHIH
                     VHAHNENKFPFTMMLGLCIHSFLEAMPLASMHHSGDNLLFAIVLHHIPIAIALMSMLV
                     ASHISRVKSIICLIIFAAMPP"
BASE COUNT          686 a          374 c          441 g          783 t
ORIGIN      
        1 catgctcgat acataaatat ccatcaaact tgccgtaatt tctttgtaac tatctaccat
       61 atctaaaacc tgaattatat gatcataagt atcttttaaa tatggactaa ccgaaacatt
      121 aataaagtca ttatgacttc ttagtatatc gtttacctta tcgcgttcgg caaatgcagc
      181 acgtctgaat aaaattaaat cgcgcttaat aatttgaatt ttttccattg atagttttgt
      241 aggctttgtc aataactcat cttctaattc atctaaagca tcacccattt tttcaagtaa
      301 tggaaagtag ctgtcaacaa ttgcatcgtt aatagcatag gtaagatagc cgcttccggc
      361 agtacgcatc atcccgcgtc cggcttttaa acgattaacg attacttcaa aaacagagtc
      421 atagtttgct tgcagggtga taacaaaatt gtttccacaa aaaactgata actggtcgtt
      481 ttgtaataaa ccttcttcac ttctgcctaa taaacgagcg ataataaata aatgtgattg
      541 gtgttcttca aacttaggcc gctgatacaa cttggtaaca tcttccattt cgaggggatg
      601 aataccaaat gcactgctta tggtttcgag caattgctta tcgccaatgc cttttatttg
      661 taaccaatag ttatgagccg ggtttgcttt aattaaagtt actgccgtgt ttattgaaat
      721 gtcagctatg gtttcgaaaa cctcggtgtt gtagccatac aatatgagtt tgggttttga
      781 agcatcatcg gcaaccacaa tagtgccggg cgaagtgccg ggtggtgtgt gatgcaccac
      841 cttgttttta gtacgttttg attttttctt gacggccacg ttgcaaaata tttggtgcta
      901 aaataaggct ttgcaaattg tagaaatagt aggtttaata tagttatgaa tactgaccat
      961 tttatttgat ttgcattaaa attaattgaa tagggcattt atgctgtaac atatatggtg
     1021 aattggtata acaaatggtt tggcacacgt tattacgact tgttgtataa caagagagat
     1081 gatttagaag ccaagcactt tttagataca ttggttaaac aacttggttt agagccgcaa
     1141 gcgcaaatat tagatgtagc ttgtggtaaa ggccggcatg ctatttacct gaataaactt
     1201 ggttttaatg taaccggtat tgacctgtcg gacaacagca ttgcacatag taaacaattt
     1261 gaaaatccat cgttgcattt ttatgtacat gatatgcgca gattattcag aaccaacagt
     1321 tttgatttgt tgctcaacct gtttacaagt tttggctatt ttgaaagaca acaccacaat
     1381 gcaatggcca taaatgcctg ggccgcagct ttaaagtccg gtggtaaatt ggtaattgat
     1441 ttttttaatg ttcaaaaaat tgccatgcag ttggtacctc atcaaacaaa acaaattgat
     1501 ggcattggtt tcaatatttc taaatcgttt gaaaatggat acatcaccaa aacaattaaa
     1561 gtaaatgatg ataatataga gaagtatttt tatgagcgtg tgcaagcgtt aactttaaac
     1621 cagtttgaaa tttatttcaa aaatgccggt ttaaaaattg attctatttt tggcgattac
     1681 gatttgaata gttttgtacc aaccgattcg gaccggttaa taataattgc aaagaagtaa
     1741 tgctattaca tggagaagta gctacgcagg tagcagcaca tataggatgg atggggtata
     1801 gcgccctgtt tttggtggtt ttgataagtg gccttggttt tttatttttt aaagaaataa
     1861 agccggccaa tttaaaactg atgttaagtt ttaccggttc gtttttattt gcattgtgtg
     1921 taatgcatat gatacccgaa gtatatgaag ccgatagcca cattggttta tatattctga
     1981 ttggtttttt ttcacaactg ctgcttgaat ttttaagtga agggattgag catgggcata
     2041 tacatgtgca tgcgcataat gaaaacaagt ttccgtttac gatgatgttg ggtttgtgta
     2101 tccattcgtt tttagaagcc atgcctttag caagtatgca tcacagtggt gataatttgc
     2161 tttttgctat tgtattgcat catataccta ttgccattgc attaatgagc atgttggttg
     2221 catcgcatat aagccgcgtt aagtcaataa tttgtttaat aatatttgcg gctatgcccc
     2281 cttt
//
LOCUS       DAOMAA010000296         2282 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_43996_length_2282_cov_2.546026, whole genome shotgun sequence.
ACCESSION   DAOMAA010000296 DAOMAA010000000
VERSION     DAOMAA010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43996_length_2282_cov_2.546026"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1737)
                     /locus_tag="PLO59_04795"
     CDS             complement(<1..1737)
                     /locus_tag="PLO59_04795"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HQV00455.1"
                     /translation="MKNILLLLIGITGQVFAQLQYPVAKKTNQTDVYFGQTVADPYRW
                     LETDDVDTKQWIADENNVTHTYLNQIPFRDSVQNKLEKLWNFPRYRPSFKVGSAYFYL
                     RNNGLQNQDVLYYKRGLDFVGMLFLDPNTVDTTGLTSLSGFSGNKNGAFIAFNVSRAG
                     SDWQQIRIKDIKKSRTLADSIDWVKFSQIAWKDSGFFYSRYDAPVDGDKFKAQNTNHK
                     VYYHRVGLPQQTDLLVYSDPTKPKCTFRCQTSSDERYLIINGAESTSGNNIIIKDLSH
                     SNSPFITIVSDFNHSYKVVDIINQKLIVQTNDEAKKGRLISIDINNIDKKNWTTIIPE
                     SADVMQEVHQADKNYLVKYMHMASNKILGYSNTGKFLYEVKLPGLCTVNEINSSVNDS
                     VFFYNITSFTIPTTVYMGYAKNGRSMIDGYDPTKKIAFRKDDYITKQVTYKSKDGTEI
                     PMFIVHKKNLQLNGNNPTLLHGYGGFNISKTPEFRAERLLFLEQGGIFAMPNLRGGGE
                     FGDAWHKAGTKLNKQNVFDDFIAAAQYLIDSKYTSAEKLAINGGSNGGLLVGACITQR
                     PDLFKVALPSVGV"
     gene            complement(1835..>2282)
                     /locus_tag="PLO59_04800"
     CDS             complement(1835..>2282)
                     /locus_tag="PLO59_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HQV00456.1"
                     /translation="VYTSYGTIGWLPDLNKWAAVVAQCLKPGGTFLIIDFHPVLWMLD
                     NEMKMLQYPYFNVDPIIETNLGSYADRNAVIKTTNIGWNHSLSDIITALIDNGLKLEK
                     FSEFDYSNYNCFKNTVQIDNGKYQIKNLQGVLPMMYAIKCSKPKLL"
BASE COUNT          661 a          429 c          403 g          789 t
ORIGIN      
        1 tacgcctacc gaaggtaagg ctactttaaa caaatcgggc cgctgtgtaa tgcatgcacc
       61 taccaataaa ccaccattac taccaccgtt gattgctaat ttttcggcac tcgtgtattt
      121 actgtcaatt aaatattggg ctgcagcaat aaaatcgtca aacacatttt gcttgtttag
      181 ttttgtaccg gctttatgcc aggcatcacc aaactcgcca ccaccgcgca agttgggcat
      241 tgcaaatatg cccccttgtt ctaaaaatag taaacgctcg gctctgaatt caggtgtttt
      301 actaatatta aatccgccat agccatgtag cagtgttggg ttgttaccat ttaattgcag
      361 gttcttttta tgtacaataa acatgggtat ttcagtaccg tctttgctct tatacgtaac
      421 ttgtttggta atataatcgt cttttctaaa tgctattttt tttgtggggt cgtagccatc
      481 tatcatactt cggccatttt tagcataacc catataaacc gttgtaggta tagtaaatga
      541 tgttatgttg taaaagaaaa cactatcgtt taccgaactg tttatttcgt taacggtgca
      601 taagcccggc agttttactt catataaaaa tttgccggta ttactgtagc ctaatatttt
      661 attactggcc atgtgcatat attttacaag atagttttta tcagcctgat gcacttcttg
      721 cattacatca gcactttcgg gtatgatggt ggtccagttt tttttatcaa tgttattaat
      781 atcaatactt atcagtcgcc ctttttttgc ttcgtcattg gtttgtacta tcagtttttg
      841 attaatgata tcaaccactt tgtagctgtg gttaaagtcg ctaactatgg taataaacgg
      901 actgtttgaa tgacttaaat ctttaataat tatattgtta ccactggtac tttcggcccc
      961 gttaataatt aaatatcgct cgtcagaact ggtttggcaa cggaatgtgc atttaggttt
     1021 ggttgggtcg ctatatacaa gcaaatcggt ttgttgcggt aaacctacac gatggtaata
     1081 aactttgtga ttggtatttt gtgctttaaa tttatcgcca tctaccgggg catcatatct
     1141 ggaataaaag aagcctgaat ctttccaagc tatctgacta aactttaccc agtcaattga
     1201 gtcggcaagg gtgcgcgatt tttttatatc cttaattctt atttgttgcc agtcgctacc
     1261 tgcccgcgaa acattaaaag caataaatgc cccattctta tttccactaa atccacttaa
     1321 agaagttaag cctgttgtat caacggtatt ggggtctaaa aataacatac ctacaaagtc
     1381 taatccgcgc ttataataaa gtacatcttg attttgtaat ccattattgc gtaaataaaa
     1441 gtaggcgcta cctactttaa atgatggccg atagcgtgga aaattccata gtttttctaa
     1501 tttgttttgt acgctatcgc gaaatggtat ttgatttaaa tacgtatgcg ttacattatt
     1561 ttcatcggca atccattgct tggtatctac atcatcggtt tctaaccagc gatatgggtc
     1621 ggcaacggtt tgcccaaaat atacatcggt ttgattggtt ttttttgcaa ccggatattg
     1681 caattgtgca aatacttggc ctgttatgcc aataagtaat aacaagatgt ttttcatagt
     1741 ttatttatag aaaggagcac aaagttaaaa acactgaaac gggttacagt tataatttgg
     1801 tgaacataaa ttgatgttcc ttttaaaaaa caaattataa aagctttggt ttgctacatt
     1861 taatggcata catcatgggt aacacacctt gcaggttttt tatctggtat ttcccattat
     1921 caatttgcac ggtattttta aaacagttgt agttgctgta gtcaaactcg ctaaactttt
     1981 ccagttttaa gccgttatca ataagcgcag taataatatc agacaggcta tggttccatc
     2041 cgatgttagt ggttttaatt acagcatttc tgtcggcata actacccaaa ttagtttcaa
     2101 taattgggtc aacgttaaag taggggtatt gcagcatttt catttcatta tccaacatcc
     2161 atagcaccgg atgaaaatca ataattaaaa acgtaccccc tggttttaaa cattgtgcaa
     2221 caacagcagc ccatttattt aaatcaggca accagccaat agtaccatag gatgtataaa
     2281 ca
//
LOCUS       DAOMAA010000297         2280 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_44062_length_2280_cov_2.669663, whole genome shotgun sequence.
ACCESSION   DAOMAA010000297 DAOMAA010000000
VERSION     DAOMAA010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44062_length_2280_cov_2.669663"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..769)
                     /locus_tag="PLO59_04805"
     CDS             complement(146..769)
                     /locus_tag="PLO59_04805"
                     /inference="COORDINATES: protein motif:HMM:NF021759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HQV00457.1"
                     /translation="MTRDELKSKLVNYLPPLAIDDCIDYISKYRIKMVVTKARSSKLG
                     DYTPPQNGHTHVITINYNLNKYAFLVTFLHEVAHLLAFINHGPQIEPHGKEWKLTFAQ
                     TLKPFIESHIFPDDVKFALMRSIQNLAASSCADSNLYRALKKHDTQGIYQLLENLPDG
                     TEFKLKGYSDVFIKGKILRKRYECALKNTERKFHIHGLAEVIQLTLL"
     gene            complement(1162..1608)
                     /locus_tag="PLO59_04810"
     CDS             complement(1162..1608)
                     /locus_tag="PLO59_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00458.1"
                     /translation="MQNSIKKLSNWAIAIFIFIACNANAQFIKDLPLGQHGGHVFTTS
                     VNNHKVECKVNDNNIELFVVDAANKDAQLKVAAATANVLFDYDGKQEPIIKTITITSK
                     NKFIVDLPKSEYKLNFIAAQFNFNGNLFESRYVVNKANSGSTSTVE"
     gene            1697..2140
                     /locus_tag="PLO59_04815"
     CDS             1697..2140
                     /locus_tag="PLO59_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00459.1"
                     /translation="MNIIRLTFFISVFACLTGCKKNQGLIDNVPVDILIYLNQPAYVK
                     LQTPGGWAYLNGGVKGIIVYNAGGNIFRAYDQSCTYDPNSSCSKVMVTADNITAVDSC
                     CGGDACCKSKYSIGNGEPLAGSSTNALVQYRTFLSADGSQLSIVN"
BASE COUNT          689 a          356 c          409 g          826 t
ORIGIN      
        1 cattaggcga tcaagaaaaa attcaatcag ttaaaaaaag tttggcttca aattaaggta
       61 taaagcaatt gtgctaatag caaaagttaa taagtaggct atttgtttaa aagtacttgc
      121 tttctttttc agcaatatgc tgtttttaca ataaggttag ttggattact tcggccaaac
      181 catgtatgtg aaacttgcgt tcggtatttt ttaaagcgca ttcgtaacgt ttgcgcaaaa
      241 ttttgccttt tataaaaaca tcgctataac cttttaattt gaattcagtg ccgtcaggta
      301 agttttcgag caattgataa ataccttgcg tatcatgttt tttaagagct ctatataaat
      361 tagaatcggc acagcttgat gcagctaaat tttgaataga acgcatcaaa gcaaacttaa
      421 catcatccgg aaatatgtga ctttcgataa atggttttaa agtttgtgca aaagtaagtt
      481 tccattcctt gccatgtggt tcaatttggg gaccatggtt tataaaagcc aaaagatgag
      541 ctacttcatg taaaaaagta actaaaaatg cgtatttatt taaattataa ttaatggtta
      601 taacatgtgt gtggccattt tgtggaggtg tataatcgcc aagtttgctg ctgcgcgctt
      661 tggttacaac catttttatg cggtatttac ttatatagtc tatacaatca tcaattgcta
      721 atggaggtaa ataatttacg agtttggatt ttagttcatc acgtgtcatt caatatcact
      781 ttggtaatta acttgttagc ctttgccaga gtgaaggctt tttatagggt gttttagctt
      841 gtttccgatg ttttttcata cgctttcggg tttcgcgcgt ttgaattttt tgatgttttt
      901 tttttgcttt tatctccgat ttgcgtgcct tttgtttttg ctcttccttt ttttgtcggc
      961 ttttttttgc gcttggctac tactttgtgc aatagactta tgtgggataa gcatgggtgt
     1021 agctgcaagt aaccacacag tgctaaaact tactaaacaa aactgtttta ccattatacc
     1081 aaattatcag aagcttatag gtcaaaaaaa aatacctttc agaatatgtc cgaaaggtat
     1141 tttttacttg taataaatac tttattcaac ggtagaagtt gaaccactgt ttgctttgtt
     1201 aaccacgtag cgggattcaa ataaattacc attaaaatta aactgtgctg caataaagtt
     1261 taatttatac tccgacttag gtaaatcaac tataaattta ttttttgatg taatggtaat
     1321 agttttaata atgggctctt gtttaccatc gtaatcaaat aatacgtttg ctgtagctgc
     1381 cgcaacttta agctgggcat ctttattagc tgcatcaaca acaaacaact caatattgtt
     1441 gtcattaact ttacactcaa ctttgtggtt gtttacactg gttgtaaata catgcccacc
     1501 atgctgtcct aatggcaagt cctttataaa ctgagcgttt gcattacagg caataaatat
     1561 aaaaatggct atagcccagt tgcttaattt tttaatggaa ttttgcatga ctttatttat
     1621 ttaaataata tctaacaaaa ttagcaataa tttgcctcgt tttaaatatt gtaacacttt
     1681 aacattaaaa aatataatga acattatacg cttaactttt tttatatctg tttttgcatg
     1741 ccttacaggt tgtaaaaaga atcaggggtt gatagataac gtacctgtag atatattgat
     1801 ttatctgaat cagcctgctt atgttaagtt gcaaacccct ggaggttggg cttacttaaa
     1861 cggtggtgtt aaaggcatta tagtttataa tgccgggggc aacatttttc gtgcttacga
     1921 ccaatcgtgc acttatgacc caaattcatc gtgcagtaaa gttatggtta ctgctgataa
     1981 tattacggct gttgattcat gttgtggtgg cgatgcatgt tgcaaatcga aatatagcat
     2041 aggcaatggc gagccgttgg ctggatccag cacaaatgca ttggttcaat atcgtacatt
     2101 tttaagtgcc gatggatcgc aattatcaat agtaaattaa taacaaaggc cgcttataaa
     2161 gcggcctttg ttattaattg tttggtatga attataattt aataaagcgt ttggcaatac
     2221 cttcaacacc tttaatgtgt acagtataaa cgccttttgg taaagatgaa atattgatat
//
LOCUS       DAOMAA010000298         2279 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_44099_length_2279_cov_2.708183, whole genome shotgun sequence.
ACCESSION   DAOMAA010000298 DAOMAA010000000
VERSION     DAOMAA010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44099_length_2279_cov_2.708183"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="PLO59_04820"
     CDS             <1..447
                     /locus_tag="PLO59_04820"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HQV00460.1"
                     /translation="ARSLILYAYAYSAASIGAFAFLIILINQLQTDHQDALKGLAYSN
                     PLLAIFLAVILISLAGIPPTAGFFAKYYLFTTLISQGYVGLVIIAVLSSLIGVYFYLR
                     PIIFMFQHSNVETKLKISQSSLAFFALVTLIIVILGFMPQLIIEII"
     gene            683..>2279
                     /locus_tag="PLO59_04825"
     CDS             683..>2279
                     /locus_tag="PLO59_04825"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HQV00461.1"
                     /translation="MYSQRNIELLKFQLAFLLILLASYAYASPVSFTLTAQTSSYGSY
                     QISCNGSNDGFIDLTVTGGTAPFVFAWSNGSTTEDINNLSAGTYKVIVTDATLFIDSM
                     EVTLSEPLPISIAVLNKKDVSCFNGVDGAIDIDVSGGVPIYTYQWSSGEITQDITAIA
                     VGTYTITVTDNQSCTVSSVFNISEPAPINLSMPASNEFCSLSNGSVTALVSGGVGGFT
                     YAWSNGATNASINFLNAGSYTVTVTDLNLCSVTGSVTLINFASPQITVDSTKIVSCIG
                     GIDGAAYITVSAGTLPYSYLWSNGNTNQDLVNVSAGTYTVTVTDGVNCTAQTIVVVNQ
                     LAELQIILNQQDDSCGNNGGYIAASVFGGVSPYSFLWSNGATSDSIFGLSAGTYTVTV
                     NDLNCSVKAQATINANAPLLVSLIQLKNVTCFGATNGDINIDATGNGPYTYTWSNGKT
                     TQDNSSIGAGVYTVTVTNSFGCSVVKVYTVTQPPPITVSSIVDSATCSQSNGSISVIA
                     SGGTGLLSYLWSTAAVTNTITNLV"
BASE COUNT          685 a          368 c          427 g          799 t
ORIGIN      
        1 gcacgttcat taattttata tgcctatgct tatagtgcag ccagtattgg tgcttttgct
       61 tttctgatta tacttattaa tcaactacaa accgaccatc aggatgcttt aaaaggttta
      121 gcatatagca acccgttatt ggcgattttt ttggcagtta tattaattag tttagcaggc
      181 ataccgccta cagcaggttt ttttgcaaaa tattatttgt ttaccacact tatcagccaa
      241 ggctatgttg gcttggtaat tattgcagta cttagttcgc ttataggtgt ttatttttat
      301 ttaaggccta ttatatttat gtttcagcat tcaaatgttg aaaccaaact taagatatca
      361 caatcaagcc tggccttttt cgctttagtt acattgatta tagtaatctt aggttttatg
      421 ccgcaattaa ttattgagat tatataaacg cgtattaatt ttaatggcaa aactactttt
      481 cattacgaac taaagtaaaa tttaaaacct actttaattt atagttgcta agtaatttct
      541 cgaataataa aataacatga gttaactatc tataaaccta cgcgaaaaaa ttaaaaacaa
      601 attcaaatca actgtgtaaa attggtataa aatatgtttt taatttacct acttacatca
      661 aaaacatatt agttttagct tcatgtattc ccaacgaaat attgaattgc ttaaatttca
      721 acttgcattt ttattgattt tattagcttc ctatgcgtat gcatcgcctg tatcatttac
      781 gttaacagcg caaacttcga gttatggtag ctatcaaatt tcctgtaatg gaagcaatga
      841 tggatttatt gatttaacgg ttaccggtgg tactgcacct tttgtttttg cgtggagtaa
      901 tggttctact acagaagata ttaacaatct ttcagcaggt acctataaag taattgttac
      961 agatgcaact ttatttatag attcaatgga agttacacta agtgagccgt tgccaatttc
     1021 gattgctgtt ttaaataaaa aagacgtttc ttgtttcaat ggtgtagatg gtgcaattga
     1081 tattgatgtt agcggtggtg tacctattta tacatatcaa tggtcaagcg gtgaaattac
     1141 ccaggatatt acagcaatag ctgtcggaac gtacactata actgtaactg ataatcagag
     1201 ttgcacagtt tcaagtgttt ttaatatttc agaaccagca cctatcaatt taagtatgcc
     1261 ggcttcaaac gagttttgta gtttatcaaa tggtagtgta actgcattag taagtggtgg
     1321 tgttggtggt tttacttatg cttggagtaa tggggcaacc aatgcttcga ttaatttttt
     1381 aaacgcaggt tcttatacag taactgttac cgaccttaat ttatgttcgg ttaccggctc
     1441 agttacgtta ataaattttg catctccaca aattacggtt gacagtacaa aaattgtttc
     1501 ctgtattggt ggtattgatg gagctgccta tataacagta tcggcaggta cgttgcctta
     1561 tagttattta tggagtaatg gaaataccaa tcaggatctg gtaaatgtta gtgccggtac
     1621 ctatacagta acagttacag atggcgtaaa ctgtaccgca caaactatag ttgtggtaaa
     1681 tcaattggct gagttgcaaa ttatactaaa ccaacaggat gatagttgcg gtaataatgg
     1741 tggatatatt gcggcaagtg tttttggagg cgtatcgcct tactcttttt tatggtcgaa
     1801 tggtgcaact tcagattcga tttttggatt aagtgccgga acctatacgg taacagttaa
     1861 tgatttaaat tgtagcgtta aagcccaagc cactattaat gcaaacgcac cattgcttgt
     1921 ttcgttaatc cagcttaaga atgttacttg ttttggtgca accaatggtg atattaatat
     1981 tgatgcaact ggcaatgggc catacacata cacttggtca aatggaaaaa caacacaaga
     2041 taattcaagt attggagccg gagtttacac cgttacggtt accaattcat ttggttgttc
     2101 tgtagttaaa gtttatactg taactcagcc accacccata accgtttcca gtatagttga
     2161 tagtgctact tgctcgcaaa gtaatggcag tattagtgta attgcttcag gtggaacggg
     2221 gttattatct tacttgtgga gtaccgctgc tgttacaaat actattacaa atttggtgg
//
LOCUS       DAOMAA010000299         2278 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_44136_length_2278_cov_3.028790, whole genome shotgun sequence.
ACCESSION   DAOMAA010000299 DAOMAA010000000
VERSION     DAOMAA010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44136_length_2278_cov_3.028790"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="PLO59_04830"
     CDS             <1..259
                     /locus_tag="PLO59_04830"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV00462.1"
                     /translation="EINIPTTSKQASLIIYPNPATNTLHISGISHKSIVKIYDVLGKL
                     IIEKESEYNMTLNTSQLSAGIYTIITQSNNSRSINKVVITK"
     gene            complement(741..1322)
                     /locus_tag="PLO59_04835"
     CDS             complement(741..1322)
                     /locus_tag="PLO59_04835"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HQV00463.1"
                     /translation="MVSKNLLSIVLVLVVAVFYGNTLRNEFALDDLIVLTGNKFTQQG
                     FAGIPDLLTHDAFVGAYGEALNLSGGRYRPFSMVMFAIEKEIFGYTPVVFHFFNVEKK
                     QVKVFEDDILFIESLKDYVRIHTSKRIFITKFQIGEIEKILTHENFIRIHKSYIVNSN
                     MITSFNAQEIEIDDISLPLGRSYKVIVERKLKG"
     gene            1388..2146
                     /locus_tag="PLO59_04840"
     CDS             1388..2146
                     /locus_tag="PLO59_04840"
                     /inference="COORDINATES: protein motif:HMM:NF018917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HQV00464.1"
                     /translation="MEFIKQVIIGVHTYVLAHKLIVKHRLWWYIIAPGIINCLLFILG
                     FSWFSSMATDWAAIFTLYIKNLAPWATWLHTGLLAWLIAFLIKVGFIIFYLSVYKYLI
                     LVLMAPLLALLSERVEFIITGHTTDFNFQQFIADVLRGILLALRNFCIEMLLLALLFI
                     CSFIPLLQLFSPVLAFYISSYYYGFSMLDYSNERRKYTIARSIAFTRLQKGVAFGNGA
                     IFYLLLIVPYVGWVIAPAYAVTAATIARLQIAKD"
BASE COUNT          736 a          404 c          366 g          772 t
ORIGIN      
        1 agaaattaat attcctacga ccagcaagca agctagtcta ataatttatc ctaatccagc
       61 tactaacact ttacacataa gtggtatcag ccacaaatca attgttaaaa tatatgatgt
      121 attaggtaaa cttataattg aaaaagaatc ggagtataat atgactctta atacaagcca
      181 actctctgca ggcatttata caatcattac acaaagtaat aatagtaggt caataaataa
      241 agtagttatt actaaataaa ttaccaaatc cgtacaatcg acaagcttag attttcagaa
      301 gtttatagtg aaattagtga cataaaaata caaaatgaaa tagtagttgc attgtgtttt
      361 taccttggaa taaatcgaac ataagcacct accacttaca taagatcaag aactctgata
      421 aaagattaac aaaacaagct tgctgatcaa gcaatgatta ttttattaca tccgaacaaa
      481 tttgaatatg cagcatgctt tgttataaaa agttataaaa aattacttag aagggataat
      541 tctttatgaa tctcctttga gttcaaatct ggatgctttg caaatagttt aaaagcatat
      601 taaatgatcc atttaattta ctcaaagtaa ttcgttcgaa aaagcatttc atatttaagt
      661 agacttattt atgaccaaag ttggaggctt gatatacaac tgcatattgc acgcattttt
      721 ataataatca taatgggagt tcatcctttt aattttcgct cgactattac cttataactc
      781 ctacctaaag gaagagaaat gtcgtctatt tcaatttctt gtgcattaaa agaagtaatc
      841 atgttgctat taacgatata ggatttatga atacgaataa agttttcatg cgtcaatatt
      901 ttttctattt caccaatctg aaatttagta atgaagatcc gcttactggt atgaattcgc
      961 acataatctt tcaaactttc aataaaaagg atatcatcct caaatacctt cacctgtttt
     1021 ttctctacat taaaaaaatg aaataccaca ggtgtgtatc caaaaatttc tttttcgatg
     1081 gcaaacatta ccatactaaa cggacggtac ctacctccac ttaaatttaa cgcttcacca
     1141 taagcgccta caaatgcatc gtgggttaat aagtcgggta tgcctgcaaa accttgctgg
     1201 gtaaacttat tgcctgtaag cacaattaaa tcatccaatg caaattcgtt gcgtaatgta
     1261 ttgccataaa aaacagcaac aacgagcacc aaaacaatac tcagtaaatt tttagaaacc
     1321 attttacttt taattacaat gtgccaatgt aaaaaacagc acaacttaat ttaatttatt
     1381 tgcccttatg gaatttataa agcaagtaat tattggtgta catacttatg tgctggctca
     1441 taaacttata gttaagcacc ggttatggtg gtacattatt gccccgggta ttattaattg
     1501 ccttttgttc atattggggt ttagctggtt tagcagcatg gctaccgact gggctgctat
     1561 ttttactttg tatattaaaa atctggctcc ctgggctaca tggctgcata ccggattgct
     1621 ggcctggctt attgcttttt taatcaaagt aggttttatc atcttttatc tgtcggttta
     1681 taagtatttg attttagtgc taatggcacc cttgctggca ttactaagcg agcgtgttga
     1741 gtttataata acaggccata caactgactt taattttcag caatttattg ccgatgtatt
     1801 acgcggtatc ttattggcgt tgcgcaactt ttgtattgaa atgttgctgt tggcgctttt
     1861 atttatttgc agttttatac cgctgcttca attattcagt ccggttttag cgttctatat
     1921 atccagctat tattatggct ttagcatgct cgactacagt aatgaacgta ggaaatatac
     1981 cattgcgcgc agcattgcat ttacacgttt acaaaaaggt gtagcctttg gtaatggagc
     2041 cattttttat ttgttgctta tagtaccata tgttggatgg gtaattgcac cggcttatgc
     2101 cgtaactgcg gcaaccattg cacgattaca aattgcaaaa gattgatgtt tgactcagat
     2161 aattttcagc agttaaacat tgtattcttt tttatattcg tagcttctac attttaacct
     2221 atgctacacg ctacacgcta cacgctacac gccttgcgct gtgcctaaga gcagcagc
//
LOCUS       DAOMAA010000300         2276 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_44211_length_2276_cov_2.786583, whole genome shotgun sequence.
ACCESSION   DAOMAA010000300 DAOMAA010000000
VERSION     DAOMAA010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44211_length_2276_cov_2.786583"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1164
                     /locus_tag="PLO59_04845"
     CDS             <1..1164
                     /locus_tag="PLO59_04845"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HQV00465.1"
                     /translation="LNTIKPDAVYKQLCMVPYWKRGAAEKAFLIKGDKKTEVNICALG
                     GSIATPKGGLTAEVVEINSWDELKQLGEKNALKNKIVFYNMPWNQTHINTFEAYGAAV
                     EPRWAGAFRAAPYGAVGVVVRSAASSVDAFPHTGSMSYKDSVNKIPACAISTIHADLL
                     SQQLKLRGMPQVKFSFTQNCVQLPDTSSFNVIGEIQGSEFPDEIIVVGGHLDAWDNGQ
                     GAHDDGAGVVQCMEVLQIFKTLNIKPKRTIRCVLFMNEENGGRGGAKYASEAKLKNEN
                     HIAAIESDAGGFVPRGFSFEGDSLAIAKIRSWQNLFTPYNIYSWDGYGSGADVGHLAG
                     NCKVLMGLSPDSQRYFTLHHTAADTFDKVNKRELQLGAATMAAMVYLLSEYGL"
     gene            complement(1266..>2276)
                     /locus_tag="PLO59_04850"
     CDS             complement(1266..>2276)
                     /locus_tag="PLO59_04850"
                     /inference="COORDINATES: protein motif:HMM:NF014869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HQV00466.1"
                     /translation="NDWLVGADRGAIRSFISPNTYGDPDTYNGTNYKTTVGCTPSSGN
                     DQCGVHSNSSIQNHMFYLLSNGGSGTTDFGVAYNVSGITRFTARDIGYRALVFYLSGS
                     DGFIDAREAWLRAAFDLYGGCSNEIIQVGNAWRAVGVESQSAQYNVNACGTYPAAGTY
                     RQAIHQLSVANGCATNITASATTVYFSATDRVVLYPGFNAVSGSKFYAYIEPCAISLY
                     KNALADNRSDAEKGLVADLQNAPDLSNGRIGTEATAINVAPNPFNNEVTVQLQLKESG
                     MVTVAIYDALGNLISTIKPNEMLAAGRQQIKYDSHHLAKGIYLMHVIIDGETSVRKIV
                     KM"
BASE COUNT          678 a          463 c          479 g          656 t
ORIGIN      
        1 ttaaacacca taaaacctga tgctgtttat aaacaacttt gtatggtacc ttactggaag
       61 cgtggtgcag ccgaaaaagc atttttaatt aaaggtgata aaaaaaccga agtaaacatt
      121 tgcgctttag gtggcagcat tgcaacaccc aaaggtggat taacagccga agtggttgaa
      181 ataaacagtt gggatgagtt gaagcaatta ggcgaaaaaa atgcactcaa aaataaaatt
      241 gtgttttaca atatgccttg gaatcaaaca cacataaaca catttgaagc ctatggcgct
      301 gccgttgaac cccgttgggc cggtgcattt agggctgcac cttatggtgc tgttggcgtt
      361 gtggttcgaa gtgcagccag tagtgttgac gcatttccgc atactggcag tatgagttac
      421 aaggattcag taaataaaat acctgcctgt gccataagta caattcatgc cgatttatta
      481 agtcagcaat taaagttgcg tggtatgccg caggtaaaat tttcgtttac acaaaattgt
      541 gtgcaattgc ccgataccag ttcgtttaat gtaattggtg aaatacaagg cagtgaattt
      601 cctgacgaaa ttattgtggt cggaggtcat ttagatgcat gggataatgg ccagggcgcc
      661 catgatgatg gagcaggtgt ggtacaatgt atggaagtgt tacaaatatt taagacatta
      721 aatattaagc ccaagcgcac catacgatgt gtgcttttta tgaatgaaga aaatggtggc
      781 cgtggtggcg ctaaatatgc atcagaagcc aaacttaaaa acgaaaatca tatagcagcc
      841 atcgaatcgg atgccggtgg ttttgtgccc cgtggcttta gtttcgaagg cgatagtttg
      901 gctattgcca aaattagaag ttggcaaaat ttatttacac catacaatat ttactcatgg
      961 gatggctatg gaagtggtgc cgatgttggc caccttgccg gtaactgtaa agtacttatg
     1021 ggtttaagcc ccgactcgca acggtacttt accttacacc ataccgcagc cgacaccttt
     1081 gacaaagtca acaaacgcga attacagtta ggtgcggcaa ctatggctgc aatggtttat
     1141 ttgttaagcg aatatggatt gtaagtaatt tgaattttac agattgctat ttatacgcta
     1201 gggacaaaaa aaacagaggc ctcagttatg agtgcctctg tttcaaaata attgtcaatt
     1261 taaacttaca tttttacaat tttcctaacc gatgtttcgc catcaataat tacgtgcatt
     1321 aaataaatgc ctttagccaa atggtggcta tcgtatttta tttgctgcct tccggctgca
     1381 agcatttcat tgggtttaat ggttgaaatt aaattaccca atgcatcgta tattgctacg
     1441 gttaccatac ctgattcctt taattgtaat tgaacggtta cttcattgtt aaatggatta
     1501 ggtgcaacat taattgctgt tgcttcagta cctatgcgcc cattcgataa atcaggtgcg
     1561 ttttgcaagt cggccaccaa acccttttca gcatcacttc tgttatcagc caaggcattt
     1621 ttgtacaatg aaatagcaca aggttcgatg taggcataaa atttactgcc cgatactgca
     1681 ttaaatcccg gatacaacac tactctatct gttgcactaa agtaaaccgt agttgcactt
     1741 gccgtaatat ttgttgcgca accattggca acacttagtt gatgaatggc ttgacggtaa
     1801 gtacctgccg cagggtaagt gccgcatgca tttacattgt attgtgccga ttgcgactca
     1861 acacctacag cacgccatgc attacccacc tgaataattt cattagagca gcctccatac
     1921 aaatcaaatg cagcacgtaa ccaggcttcg cgtgcatcaa taaaaccatc gctaccgctt
     1981 aaatagaaaa ccaaggcgcg atagccaata tcacgcgctg taaagcgcgt aataccactc
     2041 acattgtatg ctacaccaaa atcagttgta ccactgccac catttgacaa taaataaaac
     2101 atgtgatttt gaatactgct gttcgagtgt acgccacact gatcgttacc gctgcttggg
     2161 gtacaaccta cagtggtttt ataattggtg ccattgtatg tatccggatc gccataagtg
     2221 tttggactaa taaaactacg tatagcgccc ctgtcggctc ctactaacca gtcgtt
//
LOCUS       DAOMAA010000301         2276 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_44219_length_2276_cov_2.313372, whole genome shotgun sequence.
ACCESSION   DAOMAA010000301 DAOMAA010000000
VERSION     DAOMAA010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44219_length_2276_cov_2.313372"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="PLO59_04855"
     CDS             <1..465
                     /locus_tag="PLO59_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00467.1"
                     /translation="LTEAQSQVANLTQQNLTLSNKVAIGSILKAVDLTTSAVRTKGNG
                     KEVEVSRSSSAEQIKVVFTILENLVAEKGAKEIYIRIMSPTGSVLTSTQETILVNGQP
                     SLFSLHESVQYNNKDTEVTAYYPKSIAFVKGKYQVEVYHNNLQIGASSFALK"
     gene            642..>2276
                     /locus_tag="PLO59_04860"
     CDS             642..>2276
                     /locus_tag="PLO59_04860"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HQV00468.1"
                     /translation="MKNILLILALLHQTVFAQNNCDVAIRKLLRQPETTNSTKPIPIL
                     VQGNPDKIKTLTEAANGLFKYAAGNISSINLPANAIANFAGNPAVIRMESRSPRMQLL
                     NDTMRVNNNINAIQQGLPPLAQPFDGSGILCGFIDAGIDFNHPDFKDSNGNTRVAWLW
                     DHKKPVGANTPAAFGYGQEWDSTAINAGQCTQNTALAYGHGSFSAGITAGNGLATGFN
                     QGCAPKADLLVVNYDFDASPGIPNAIADGINYMFTKADALGKPCVINLSLGDLYGSFD
                     GKDLEAQAIDNMLHAKPGRLIVAAAGNWGGQKAHLSYQVNADTNWTWFKYDPAFGQLY
                     IELYGDSADMVNLNFALGADANTTAASFLGSTAFTKVTQIPLSGTYKTIAGLPGDTVF
                     YDVDKFNGVYALYINIITQNPTYFWRLMTTGSGTFGLRNYLFTAGLPSGMVSDNLPSA
                     SIVPNIIHYRLPDSLASLSNSFNCLDDVVSVGNYVNMNSNYDCQNVLRFNPVSPVGDL
                     APNSSVGPTRDGRQKPDISATGNYTMAPLPMVFVNDV"
BASE COUNT          697 a          487 c          455 g          637 t
ORIGIN      
        1 cttaccgaag cccaatcaca ggtagctaat ctaacgcagc aaaatctgac tttaagtaat
       61 aaagtagcca taggcagcat attaaaagct gttgatttaa ccacatctgc agtacgcacc
      121 aaaggcaatg gcaaagaggt agaagtgagc cgatcgtcat cggccgaaca aataaaagtt
      181 gtgtttacca tactcgaaaa tttggtagcc gaaaaaggcg ctaaagaaat ttatattcga
      241 attatgagtc caacaggttc tgtacttacc tccacccagg aaacaatttt ggtaaacggc
      301 cagccatcat tgttcagttt acacgaatcg gttcaataca acaataaaga tacagaagta
      361 accgcgtatt accccaaaag tattgccttt gtaaaaggca aatatcaggt tgaagtttat
      421 cataacaacc tgcaaattgg tgccagttca tttgcactca agtaaaaaac atacccttgt
      481 tttgaaaagc cacagcctta aaaagctatg gcttttttaa tgcgtactac aaaacaatgt
      541 tgcattcgta ctacaacaac ctgtaaaagc agcacggttt atttatatta attatttact
      601 ttgaaacgca ggttattcaa ctactattaa attcaaacga gatgaaaaat attttattga
      661 tattggcttt gttacatcaa accgtattcg cacaaaacaa ctgcgatgtt gccatacgca
      721 aattgctgcg tcagcctgaa acaacaaaca gtaccaaacc cattcccata ttagtacagg
      781 gcaatcctga taaaataaaa acacttaccg aagctgccaa tggtttgttc aaatatgcag
      841 ccggcaatat ttcatcaata aatttgccgg caaatgccat tgcaaacttt gccggtaacc
      901 cggctgttat acgtatggaa agccgcagtc cccgcatgca attgcttaac gatactatgc
      961 gcgttaataa taacatcaac gctatacaac aagggttacc accacttgca caaccttttg
     1021 atggcagtgg tattttatgc ggctttattg atgccggtat tgattttaat catcccgatt
     1081 ttaaagacag caatggcaat acacgtgtgg cctggctatg ggatcataaa aagcctgtag
     1141 gcgccaatac accggccgca tttggctacg ggcaagagtg ggatagtaca gccattaacg
     1201 caggccaatg cactcaaaat accgctttgg cttatggcca tggttcgttt tcggcaggca
     1261 taaccgctgg caatggcttg gcaacaggtt ttaaccaagg ctgtgcacct aaagctgatt
     1321 tattagtggt taattacgat tttgatgcaa gccctggtat tcccaatgcc attgccgatg
     1381 gtataaatta catgtttacc aaggccgatg ccttaggcaa accgtgtgtt ataaacctaa
     1441 gcctgggcga tttatatgga agttttgacg gaaaagattt agaggctcaa gccattgata
     1501 atatgttgca tgcaaaacca ggccgcttaa ttgttgctgc tgcaggtaac tggggtggtc
     1561 aaaaagcaca tttaagttac caggtaaatg ccgataccaa ttggacctgg tttaagtacg
     1621 accccgcttt tggtcaactg tatattgaat tgtatggcga ctcggccgat atggttaacc
     1681 ttaattttgc attaggtgcc gatgcaaaca caacggctgc atcgttttta ggcagtacgg
     1741 catttactaa ggttacacaa ataccattga gcggcactta caaaactatt gccggtttgc
     1801 cgggcgatac cgttttttat gatgtagata agtttaacgg tgtttatgcg ctatatataa
     1861 atatcatcac ccaaaacccc acatattttt ggcgattaat gactacaggc agtggcacat
     1921 ttggcttgcg caactattta tttacagccg gcttgccatc aggcatggta tccgataatt
     1981 tgccatcggc atcaatagta cctaacatta ttcattaccg tttacctgat tcgcttgcat
     2041 cgctaagcaa tagctttaat tgtttagatg atgtggtttc agtaggtaat tatgtaaaca
     2101 tgaacagcaa ttacgactgc caaaatgtgt tacgctttaa tccggtaagc cctgttggcg
     2161 atttagctcc caattcaagt gtaggcccta cacgcgatgg ccgccaaaag cccgacatta
     2221 gtgccacagg taattatacc atggcaccct tacccatggt ttttgtaaat gatgtt
//
LOCUS       DAOMAA010000302         2265 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_44623_length_2265_cov_2.875113, whole genome shotgun sequence.
ACCESSION   DAOMAA010000302 DAOMAA010000000
VERSION     DAOMAA010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44623_length_2265_cov_2.875113"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="PLO59_04865"
     CDS             <1..796
                     /locus_tag="PLO59_04865"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV00469.1"
                     /translation="TSDIYSIVVDIPAFAKYEYKFVNGDQFYEAEFVPELSRVLYNFN
                     DNRWLYVDSIANDTTFVGAIMFGGNAPAGLTLIRFYVDMQNETIAAGGMHVAGNFQGW
                     STTATHLYNFDAAIWEVIAYVNAGSTYEYKFYNGATALDAETVPAICATNTNRTVDVN
                     YDTLLTVVCYAACTACPGTSAINDVTTANLSLWPNPATQAANISWNGAGSTQIVITDL
                     AGRTVRSYTNLLNNQLTINKADLTNGLYFVTCVQNNNIKKSKLIFN"
     gene            823..>2265
                     /locus_tag="PLO59_04870"
     CDS             823..>2265
                     /locus_tag="PLO59_04870"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HQV00470.1"
                     /translation="MRFLKCIVCAYCCLAQVNAQSLYDGGTIQKIEIYFSQSNWDYQL
                     DTARLGFDDYVIADWVKINGSHYDSVGVKYKGNSSYDSTYIKNPLHIELDNVKAQSHQ
                     GFTDIKLGNNYADPSGIREVLAYKILNNYMHCPQANFAQLYINSNYIGLFANVESINK
                     DFLSTHFNSSSNTFFKCNPTVTPGPTTKSNLRYIPFADSSGYFNFYELKSDYGWNELV
                     NLCDTVTNYQSNVSNVLDMDRVLWMLAFNQVLVNLDSYTGVFCQNYYLYKDASSRFSP
                     IMWDLNMAFGGFPFIGSGNTSMGSMTVTNLQQLTPIFHATDTYWPLINVVMNNAAYKK
                     EFMAHARTIFSENFSNGNYVTDAAALQLLIDTAVLADANNFFTYTQFQNGLTGDVVFG
                     SYTVPGISNLMNGRINYLQTNADFIKVPPVINAVQPSTNTPALNSLITINATVSNATQ
                     VYLGYRSNAQNAFVRTTMFDDGLHNDGAAAD"
BASE COUNT          714 a          427 c          433 g          691 t
ORIGIN      
        1 cacatcagat atatatagta ttgtagtaga tataccggcc tttgcaaaat atgaatacaa
       61 atttgtgaat ggcgatcagt tttacgaagc cgaatttgta cccgagcttt cgcgtgtgtt
      121 gtacaatttt aacgacaacc gctggttgta tgttgattcg attgctaacg ataccacctt
      181 tgttggagcc attatgtttg gcggcaatgc accggccggc ttaacattga taaggtttta
      241 tgttgatatg caaaacgaaa ccattgctgc aggtggcatg catgtagccg gtaattttca
      301 ggggtggagc actacagcta cacacctata taattttgat gccgctattt gggaagttat
      361 tgcttatgta aatgcaggca gtacttatga atataagttt tataatggag caactgcact
      421 cgatgccgaa accgttccgg caatttgtgc caccaatacc aaccgtaccg ttgatgttaa
      481 ttacgataca ttgctaacgg ttgtttgtta tgcagcatgc acagcttgcc cgggcacatc
      541 agcaataaat gatgttacaa ccgctaacct gagtttgtgg ccaaacccgg caacacaagc
      601 agccaatatt agttggaatg gcgcaggaag tactcaaatt gttattactg atttggcagg
      661 ccgcacagta cgtagctaca ctaacttgtt gaacaatcaa ctcactatca acaaagcgga
      721 tttaacaaac ggcctttact ttgttacgtg cgtacaaaac aacaacatca aaaaatcaaa
      781 actgattttt aattaatcca tatttttcaa gctacatatt acatgcgttt tttaaaatgt
      841 atcgtttgtg catactgctg tttggcacaa gtaaatgcac aatcattata tgatggaggt
      901 accatccaaa aaattgaaat atatttctca caaagtaatt gggattatca gttagatacg
      961 gctcgtttgg gttttgacga ttatgttata gccgattggg taaaaattaa cggctcgcat
     1021 tatgatagtg ttggtgtaaa atataaaggc aatagcagtt acgattcaac ctacattaaa
     1081 aacccgttgc acatcgaatt agataatgta aaagcacaat cgcatcaagg atttaccgat
     1141 ataaaattgg gtaataatta tgccgaccca agtggcattc gcgaagtatt agcatataaa
     1201 attttaaaca attacatgca ttgtccgcaa gctaactttg cacaactgta tataaactca
     1261 aattacattg gcttatttgc gaatgttgaa agtataaaca aggatttctt aagcacgcat
     1321 tttaattcaa gcagcaatac gttttttaaa tgcaatccaa ctgtaacgcc cgggccaacc
     1381 actaaaagta atttacgata catccccttt gccgatagca gtggatactt taacttttat
     1441 gagttaaaat cagactacgg ttggaatgaa ttggtaaact tatgcgacac agttaccaac
     1501 tatcaaagta atgttagcaa tgtactcgat atggatcggg tgttgtggat gttagctttt
     1561 aatcaggttt tagttaacct cgatagttat accggagttt tttgccaaaa ctattacttg
     1621 tataaagacg catcatcacg atttagtccc attatgtggg atttgaatat ggcctttggc
     1681 ggtttccctt ttattggtag tggcaacacc agcatgggta gtatgactgt tacaaacctg
     1741 cagcaactta caccaatatt tcatgctacc gatacctact ggcctttaat aaatgtggta
     1801 atgaataatg cagcctataa aaaagagttt atggcacatg cgcgcactat cttttccgaa
     1861 aactttagta atggaaatta cgttaccgat gccgctgctt tgcaattgct tattgataca
     1921 gcagtgttag ctgatgcaaa caattttttc acctatacac aatttcaaaa cggattaacc
     1981 ggtgatgtag tctttggatc ctatacggtg cctggtataa gtaatttgat gaatggccgt
     2041 ataaactatt tacaaaccaa tgcagatttt ataaaagtac caccggtaat aaacgcagta
     2101 cagccttcaa caaatacacc ggctttaaat tcacttataa ctattaatgc aactgtaagt
     2161 aatgccacac aagtttattt aggctatcgc agtaatgcac aaaatgcatt tgtacgaacc
     2221 actatgtttg acgatggttt gcataatgat ggcgctgctg ctgat
//
LOCUS       DAOMAA010000303         2264 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_44660_length_2264_cov_2.693979, whole genome shotgun sequence.
ACCESSION   DAOMAA010000303 DAOMAA010000000
VERSION     DAOMAA010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44660_length_2264_cov_2.693979"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..754)
                     /locus_tag="PLO59_04875"
     CDS             complement(170..754)
                     /locus_tag="PLO59_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HQV00471.1"
                     /translation="MKKNFLIALLALAFTNVNAQNKWNLDASHSNVNFTVSHLVVSEV
                     DGNFKIFNGDIVAKDDSFEDATINFNVDVASINTENKDRDAHLKSDDFFNAEKFPKMS
                     FKSSAFKKVNGNKYVLKGFLTIRDVTKPVSFDVTFGGVVKDPWGNTKAGFKATTTISR
                     SAYKLLWNKTIETGGAVVGDEVTITVKTEFAKAK"
     gene            complement(869..1957)
                     /locus_tag="PLO59_04880"
     CDS             complement(869..1957)
                     /locus_tag="PLO59_04880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQV00472.1"
                     /translation="MYWLIGIIFQFVYLLYSVIISIRFKNIEPYKSKSNASYSTHVSV
                     VIAYRNESANLPQLIDGLKKQTYPTQHFECILIDDHSTDESHSMVQKAIESNWQNLSL
                     PDDRLGKKAAINYGILTAKGKLIITTDADCILNPLWIESMVQYYEQYKPAMIVGPVQL
                     LANNTIGYLQQIEFLALMAITGATTSSKPLMCNAANLAFEKEAYMQCEQNLNRTASGD
                     DTFLMLAIKYKLHTNIVFIKNKHAIVKTNACNNWQTLKQQRIRWAAKTKYYTHRYIYL
                     FGLGILASNLFMAIAISFCMVSPIYCSLIILIKLLADGIIVQSSNQFFKQQIKLHHFL
                     MVYAIYPFYSIYIYYRARKNKFVWRNRN"
     gene            complement(1957..>2264)
                     /locus_tag="PLO59_04885"
     CDS             complement(1957..>2264)
                     /locus_tag="PLO59_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00473.1"
                     /translation="FNFIVHLEVVLQFLILLYALGCNFSLILLAGIAVQFLLLAIIPT
                     MVIIELPLRATTATLVLSQMGCNESAIIQATFLLWLINLTLPAIAGICITWYYKRKH"
BASE COUNT          724 a          392 c          370 g          778 t
ORIGIN      
        1 gaagttttat taattcaatt gtcctgataa aagtgttttg tgtttcgcct tggttaccac
       61 ctaacagtaa ataagctaca gccataaata ttttaattca atgcaacgaa acaaaaaaaa
      121 acgacttgct gataattgca agccgttttt tttcgcttaa tggttttaat tattttgctt
      181 tagcaaattc agtttttacg gtaatcgtta cttcatcgcc aacaacggca ccaccggttt
      241 ctatggtttt attccataac agtttatagg ccgaacggct aattgttgtt gtagccttaa
      301 aacctgcttt ggtattgccc cacgggtcct taaccacacc accaaaggta acatcaaacg
      361 atacaggctt agtaacatct ctgatagtta aaaatccttt taaaacatat ttattgccgt
      421 ttactttttt aaatgcgcta ctcttaaaag acatttttgg aaatttttcg gcattaaaaa
      481 agtcgtcact tttaaggtgt gcatccctat ctttattttc agtgttaatg ctggctacat
      541 caacattgaa attaattgtg gcgtcttcaa atgaatcatc tttagcaaca atatcgccat
      601 taaaaatttt aaaattgcca tctacctcac taactactaa atgagatacg gtaaaattta
      661 cgttgctatg gctggcatct aaattccatt tgttttgtgc atttacattg gtaaaagcca
      721 gagcgagcaa agctattaag aagttctttt tcattttaat tattgagttt aagttaaatc
      781 gggtacaaat atatgtatgt acatataata ttcaaaatca aaagccattt tattactttg
      841 ctactttaaa actcatttat tggtttattc agttcctatt gcgccaaacg aatttattct
      901 ttcgcgcacg ataatatatg taaattgaat agaaggggta aatggcatat accattaaaa
      961 agtgatgcag cttaatttgt tgtttaaaaa attgattact tgattgaaca attatgccat
     1021 cagccagaag cttgataagg ataattaaac tacaataaat tggcgacacc atgcaaaaag
     1081 aaatagcaat tgccataaat aaattgcttg ccaatatacc taagccaaat aagtatatgt
     1141 accggtgcgt gtagtactta gttttagcgg cccatctgat tcgttgttgt tttaaggttt
     1201 gccagttatt acaggcattg gtttttacta ttgcatgttt gttttttata aagactatat
     1261 tagtatgtag cttgtatttt atggctagca ttaaaaaggt atcatcacca cttgcagttc
     1321 ggtttaaatt ttgttcgcac tgcatatatg cttctttttc aaaagctaag ttggctgcat
     1381 tacacattaa tggtttgctc gaagtggttg cgcctgttat agccattaac gccaaaaatt
     1441 ctatttgttg taaatatcca atagtgttgt tagctaaaag ttgaaccggc ccaactatca
     1501 ttgcaggctt gtattgttcg tagtattgta ccatgctttc aatccacaat gggtttaaga
     1561 tacaatcggc atccgtagtg ataattagct tccctttagc agttaaaatg ccataattta
     1621 tcgctgcttt ttttcccaat ctatcatcgg gtaatgacaa attttgccaa ttactttcga
     1681 ttgctttttg caccatactg tggctttcat ctgttgaatg atcatcaatc aaaatacatt
     1741 cgaagtgctg cgtggggtag gtttgttttt tcaaaccatc aataagctgt ggtaaattag
     1801 cactctcgtt tctatacgct ataacaacgc taacatgggt gctgtacgat gcgtttgatt
     1861 tcgatttgta aggttcaatg tttttaaatc gaatcgaaat aattaccgaa taaagcaagt
     1921 aaacaaattg aaatataata cctataagcc aatacattaa tgctttcttt tataatacca
     1981 ggttatacaa atacctgcta ttgcaggtaa tgtgagatta attaaccaaa gtaaaaaagt
     2041 agcctgaata atagcacttt cattacagcc catttgactg aggactaaag ttgctgttgt
     2101 cgctcttaac ggcaattcga taattaccat agtcggaatt attgcaagca gtaaaaactg
     2161 aacggcaatg cctgccaata aaatcaggct aaaattacag ccaagtgcat aaagtaaaat
     2221 taaaaactgc agaacaactt ctaaatgaac tataaaattg aagt
//
LOCUS       DAOMAA010000304         2260 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_44818_length_2260_cov_2.793197, whole genome shotgun sequence.
ACCESSION   DAOMAA010000304 DAOMAA010000000
VERSION     DAOMAA010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44818_length_2260_cov_2.793197"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="PLO59_04890"
     CDS             <1..813
                     /locus_tag="PLO59_04890"
                     /inference="COORDINATES: protein motif:HMM:NF021604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HQV00474.1"
                     /translation="KQYIESGILERYLLGHCTTEEIQEVQCMARVFAEVQAELDAIEQ
                     ALQTYASMQAVAPPADLKAKILARLDDQPNAETSQELRETKVVPMQSSSTGNKWAIAA
                     AACFLLAMGLGYLYNNSQKQLGLLNSQMAAMQNNLDAANALTLSTRQQLQKTGNMLTM
                     INDTAIQRVSMKGLPIAQNALATIYWNKQNKQVYLNINNMPQPATGKQYQLWAIADGK
                     PVDLGVFDITNDSLLMLKMHDIQNPQAFAVTLEPTGGSVSPTLDQMYVMGAL"
     gene            986..2224
                     /locus_tag="PLO59_04895"
     CDS             986..2224
                     /locus_tag="PLO59_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007757313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015506 - nucleoside:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HQV00475.1"
                     /translation="MDIKNRLRTLNFLQFFVWGAWLISIGNYLGGTLHFTGAEIGAVF
                     STLGIASLFMPALMGIVADKYLNAERVLGLCHLLSGLLLFYASTVTQPQMFFWVMLCN
                     AMFYMPTIALNNTVSYIILEKNGYNIITDFPPIRVWGTIGFVVAMWLTDLCGWTSNAN
                     QLMFSAVSSIALSLYAFLLPACKPTKSASKSVLSAIGLDAFKLFKQRTMLVFFLFAMC
                     LGAALQITNAFGGLFLESFQSTHPDSFGVKHSNLLLSISQISETLFILTIPFFLQRYG
                     IKKVMLLSMIAWVFRFGLFAIGDPGNGVALLILSMIIYGMAFDFFNISGSLFVEQEAP
                     GQIRASAQGLFMFMTNGLGAMIGGYCSGLVVDYFTINDVRNWQAIWFAFAAYAAILAL
                     CFPFLFKYKHQPITKNQIKH"
BASE COUNT          666 a          423 c          450 g          721 t
ORIGIN      
        1 aagcaataca tagaatcagg catactcgag cgttacttgc ttgggcattg caccactgaa
       61 gaaattcagg aggtacagtg tatggctcgt gtatttgccg aagtacaagc agagttagat
      121 gccattgaac aagcattgca aacttatgca agcatgcaag cagttgcacc accggccgac
      181 ttaaaagcca aaattttagc ccgattagat gatcagccaa atgctgaaac cagccaagag
      241 ttgcgcgaaa ccaaagtggt acctatgcaa agtagctcca ccggtaataa atgggcaata
      301 gcagcagccg cttgtttttt acttgcaatg ggtttaggct atttatataa caattcgcaa
      361 aagcaacttg ggctattaaa tagccaaatg gctgcaatgc aaaacaattt agatgcggct
      421 aatgctttaa cgctaagtac cagacaacag ttgcaaaaaa ccggcaatat gttaaccatg
      481 attaacgaca ctgctataca acgtgttagt atgaaagggt tacccattgc tcaaaatgca
      541 ttagcaacca tttattggaa caaacaaaat aaacaggtgt atttaaatat caacaatatg
      601 ccgcaacctg ctaccggtaa gcaatatcaa ttgtgggcaa ttgccgatgg caagccggtt
      661 gacttaggcg tttttgatat caccaacgat tcgttactga tgctaaagat gcacgatatt
      721 caaaacccgc aagcttttgc agttacactt gagcctacag gcggcagtgt atcaccaaca
      781 ttggatcaaa tgtatgtgat gggtgcttta tagttcgggc aataattttg tttcttccat
      841 atactaaacc aagtggagca caaacatggc tcaatttcta tcacagctat gctaatttgt
      901 ttctaactaa tcatcttgac acaacaatct gatgtaggta ataatttttt ttcatcttgc
      961 ttcggttaaa taaaaccaaa gtgatatgga cataaaaaac cggttacgca cgctcaattt
     1021 tttacaattt tttgtttggg gtgcatggct tatttctatc ggaaattatt taggtggcac
     1081 actgcatttt accggtgccg aaattggagc cgtattttct acattaggca ttgcctcact
     1141 ttttatgccg gcattaatgg gcatagttgc cgataagtat ttaaatgccg agcgtgtttt
     1201 aggcttgtgt catttactta gtggattgtt attgttttat gcatcaactg ttacgcaacc
     1261 ccaaatgttt ttttgggtaa tgctttgcaa tgctatgttt tacatgccaa ctattgcctt
     1321 aaacaatacg gtctcgtaca tcatactcga aaaaaatggc tacaacataa ttaccgattt
     1381 tccaccaatt cgcgtgtggg gcaccattgg atttgtggtg gctatgtggc ttaccgattt
     1441 atgcggatgg acatcgaatg caaatcagct tatgtttagt gccgtttcga gtattgcatt
     1501 aagtttatat gcttttttat tacccgcatg caagccaaca aagtcggcat ctaaatcagt
     1561 tttatcggcc ataggtttag atgcatttaa gttgtttaaa caacgaacca tgttggtgtt
     1621 ttttttattt gctatgtgct taggcgcggc actgcaaatt accaatgcat ttggcggttt
     1681 gtttctcgaa agttttcaaa gtacgcaccc cgattcattc ggagtaaaac attcaaattt
     1741 actgttaagt atatcgcaaa taagcgaaac actttttata ctaaccattc catttttctt
     1801 acaacgttat ggaattaaaa aagtaatgct gttaagtatg attgcctggg ttttcaggtt
     1861 tggtttattt gccattggcg acccgggcaa cggtgttgca ttgcttattt taagtatgat
     1921 tatttatggt atggctttcg acttctttaa tatttcaggt tcactgtttg ttgaacaaga
     1981 agcaccaggc caaattcgtg caagtgcaca aggattattt atgtttatga caaatggctt
     2041 aggtgctatg attggcggct attgcagtgg tttagtagtt gattatttta caataaacga
     2101 tgtgcgaaac tggcaggcaa tatggtttgc atttgctgct tatgcggcaa tattagcatt
     2161 gtgtttcccg tttttattta aatataaaca tcagcccata actaaaaatc aaattaaaca
     2221 ttaatgcaaa aactcacttt agagattgcc tgttttccag
//
LOCUS       DAOMAA010000305         2259 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_44847_length_2259_cov_3.413793, whole genome shotgun sequence.
ACCESSION   DAOMAA010000305 DAOMAA010000000
VERSION     DAOMAA010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44847_length_2259_cov_3.413793"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1667
                     /locus_tag="PLO59_04900"
     CDS             <1..1667
                     /locus_tag="PLO59_04900"
                     /inference="COORDINATES: protein motif:HMM:NF014731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HQV00476.1"
                     /translation="AMIGGAALLADGFITPPISISAAVEGLRYYNPEINTVSIVISIL
                     VVIFAIQQFGTHWVGVAFGPIMFIWFSMLAVLGISQLLYHPEVLRAINPMYAYQLLAI
                     HPEGYWVLGAVFLCTTGAEALYSDLGHCGKANIRVSWIFVKCCLLLNYFGQAAWAIDH
                     SGMVLQKQDNPFYLIMPQAFLPYGIVVATMASIIASQALISGSFTLISEAMRLNLWPK
                     IRVFFPSDMRGQIFVPSINLILFVGTILVTLYFKESAEMEAAYGLAITLTMLMTTILY
                     SAYLHFKRVHILVIGLYLIVFLSIELSFFFANLVKFFHGGYVTLILAGILGMVMYVWY
                     RGRQIRNRYVDFVNLNDYMPLIDELSHDDGVPKFATHAVYLTSANNAMQVENKIIYSI
                     LQQRPKRADIYWFVHVDVLEAPHTMEYSVKFLHAKNIVRIDFRLGFKVEQKINFYFRK
                     VIEEMVERNEINVYSRYQSLSKNKVIGDFRFIVIDKLLSYDNRINVVDKFITYFFNIL
                     KKLSTSDEKSFGLDSSNVLLEKFPLLINPRQVKINRVPYRSVNDDE"
     gene            1722..>2259
                     /locus_tag="PLO59_04905"
     CDS             1722..>2259
                     /locus_tag="PLO59_04905"
                     /inference="COORDINATES: protein motif:HMM:NF036516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family peptidase"
                     /protein_id="HQV00477.1"
                     /translation="MKYTISCKKPHTRFVDVELLIQNITTDTILVKLPNWRPGRYELG
                     NFSKNVQRFSVFTLDAGNVMGYEKIDKSTWQIKTGGRNALIIKYNYYANQLDAGACFV
                     SDEMLYFNPIHCCLYVPDKIDQQHTLFIDVPQNWKIATALFKYEGGYHAENYHQLVDS
                     PVIASPTIQKQTYQSNGIN"
BASE COUNT          701 a          370 c          437 g          751 t
ORIGIN      
        1 cggccatgat tggtggtgct gcattacttg ccgatggatt tattacgcca cccatttcga
       61 tatcggctgc agtagaagga ttacgatact acaatcccga aataaatacg gtatcaatag
      121 ttataagtat tctggttgta atttttgcca ttcaacaatt tggtacccat tgggtaggtg
      181 tagcatttgg tccgatcatg tttatctggt ttagtatgct ggctgtgttg ggtatttcgc
      241 aattgcttta tcatcccgaa gttttaagag caattaaccc catgtatgct tatcaattgt
      301 tggctataca tcccgaagga tattgggttt taggtgctgt gtttttatgt acaacaggtg
      361 ccgaagcatt atattcggat ttaggccatt gtggtaaagc caatatcaga gttagttgga
      421 tttttgtaaa atgttgtttg ctcttaaatt attttggaca agctgcgtgg gcgattgacc
      481 attcgggcat ggttttgcaa aagcaagaca atccgtttta tttaattatg ccacaagcat
      541 ttttacctta tggtattgtg gttgctacca tggcatcaat tatagccagt caggcattaa
      601 ttagtggttc gtttacacta ataagcgagg ccatgcgatt aaacttatgg ccaaaaatca
      661 gggtcttttt cccatcagat atgcgcgggc aaatatttgt ccccagtata aatttaatac
      721 tttttgtagg tacaatactt gtaacacttt attttaaaga atcggccgaa atggaagcag
      781 cttatggctt agctataaca cttaccatgt taatgacaac catattgtat agcgcgtact
      841 tacattttaa aagagtacac atactagtaa ttgggttata ccttatagtt tttttaagta
      901 tagaactcag ctttttcttt gccaatttgg ttaagttttt ccatggcgga tatgtaacac
      961 tcattcttgc aggcatttta ggcatggtta tgtatgtttg gtaccgaggt cgtcaaattc
     1021 gcaacaggta tgttgatttt gtaaacctta atgactatat gccattaatt gatgaattaa
     1081 gtcatgatga tggtgtgcca aaatttgcaa cgcatgccgt ttatttaacc agtgccaaca
     1141 atgccatgca ggtcgaaaat aaaatcattt acagtattct gcagcaacgc ccaaagcgtg
     1201 ccgatattta ttggtttgtg catgttgatg ttttagaagc accgcatact atggagtatt
     1261 cggtaaaatt tttacatgca aaaaatattg ttcgtattga tttcagactg ggatttaagg
     1321 ttgaacaaaa aataaacttt tatttcagaa aggtcatcga agaaatggta gagcgtaatg
     1381 aaataaatgt ttacagccgc tatcaatcgc tcagtaaaaa caaagtaatt ggcgatttca
     1441 ggtttattgt aattgataaa cttttgtctt atgataatag aataaatgtg gtagataagt
     1501 ttattacata cttctttaat atccttaaaa aattatcaac cagcgatgaa aaatcttttg
     1561 gattagacag tagtaatgtg ctgctcgaaa aatttccgtt gcttataaat ccacgccagg
     1621 taaaaattaa tcgtgtgccc tatcgcagtg taaatgatga tgaataatgt tgtgttgata
     1681 atttatactg tatcaattgc tacgtaaaaa ttataaatta aatgaagtac accatttctt
     1741 gtaaaaagcc gcatacacgg tttgttgatg ttgagttgtt gatacaaaac ataacaacag
     1801 ataccatatt ggtaaagcta cccaattggc gccccggcag atatgagtta ggaaattttt
     1861 caaaaaatgt gcaacgcttt tctgttttta cattggatgc aggcaatgtg atgggctatg
     1921 aaaagattga caaatctacc tggcaaataa aaaccggtgg aagaaatgca ctaataatta
     1981 aatataatta ttatgctaat caattagatg ccggtgcatg tttcgtgtca gacgaaatgc
     2041 tgtattttaa tcccattcat tgctgtttgt atgtgcccga taaaatagat caacagcata
     2101 cattgtttat tgatgttcca caaaactgga aaatcgccac cgcattattt aaatacgaag
     2161 gtggctatca tgccgaaaat tatcaccagt tagttgatag tcctgttatt gcatcgccaa
     2221 ccatacaaaa acaaacctat caaagtaatg gcataaact
//
LOCUS       DAOMAA010000306         2258 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_44889_length_2258_cov_3.546981, whole genome shotgun sequence.
ACCESSION   DAOMAA010000306 DAOMAA010000000
VERSION     DAOMAA010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44889_length_2258_cov_3.546981"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="PLO59_04910"
     CDS             <1..969
                     /locus_tag="PLO59_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HQV00478.1"
                     /translation="GLPSGVWRTNLMIEGEEEVGSANLGDWIRANKERLKGDVILISD
                     TSIISNDTPSIDVGLRGLSYLEVEVTGPNRDLHSGVYGGAVANPIQVLCEMISQLKDT
                     KQHITVDGFYDDVLSVSDADRKAMALTPFDLNEYKADLGINEVRGETGYTTLERTGIR
                     PTLELNGIWGGYTGEGSKTVLPSKAYAKISMRLVPNQNHETITKLFAQHFEKMAPSYV
                     KVKVTPHHGGMPYLTETTSDAYIAAEKAMETTFGKKPIPTRGGGSIPIVALFEKELGL
                     KSVLMGFGLDSDLIHSPNEHFGIFNFLKGIETIPHFFHNYTAIKTK"
     gene            complement(1086..>2258)
                     /locus_tag="PLO59_04915"
     CDS             complement(1086..>2258)
                     /locus_tag="PLO59_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00479.1"
                     /translation="PVNFSSFNEAYTYTQPNITTNGNKGYLIHFVSNMPGGYGKMDIW
                     QMQLDLQGQFTTPINAGDAINTFADDETPFYAKTEEALYFSSEGHYGFGGFDVFRSDN
                     LTGQFKTATNLGLPLNSSYNDLYFTVNDNDTSGILTTNRKGALHINSETCCYDLYAYK
                     INRLEKQKIKGEVQKSYGVITLDEGPVSTRDSLTRSIIENELEFSFPLRLYFDNDEPD
                     PKTLKETTKADYDALFKKYQLTASTYKENYALGFAVNEREKAALRMHTFFEDDLLDGY
                     QRLEKMCQYVLSLLQRDKTVTITVRGMSSPLADSQYNFRLSQRRISCFINYLNKYNQN
                     ILQSYFDGGKLIIVKEAMGEADVVKVSDDVRNKAQSVYSPEAALARKIEVTSLSFK"
BASE COUNT          702 a          409 c          435 g          712 t
ORIGIN      
        1 ggtctcccca gcggagtgtg gagaaccaac ctcatgattg agggcgagga ggaagttggc
       61 tcggcaaact taggcgattg gatacgagca aataaggaaa ggcttaaagg ggatgtaatt
      121 ttaatttctg atacatctat tatcagcaac gatacaccca gcattgatgt gggcctgcgc
      181 ggcttgagtt atttagaagt tgaggttacc ggccctaaca gagatttgca ctcaggtgtt
      241 tatggtggcg cagttgctaa ccctattcaa gtgctttgtg aaatgatttc acaattaaaa
      301 gataccaaac agcacattac tgttgacggg ttttatgatg atgtgctatc cgtatcggat
      361 gccgatagaa aagctatggc tttaactcct tttgatttaa atgaatacaa agctgactta
      421 ggcattaacg aagttagagg cgaaaccggc tacaccacat tagagcgaac cggaatcaga
      481 cccacattag agttaaatgg tatttggggc ggttatacag gcgagggtag taaaacggtt
      541 ttaccatcaa aagcttatgc aaaaataagc atgcgattgg tacccaatca aaaccatgaa
      601 accattacaa aattgtttgc gcaacatttt gaaaaaatgg cgccatcata cgtaaaagtt
      661 aaagttacac cccatcatgg tggcatgccg tatttaaccg aaaccacaag cgatgcttac
      721 atagctgccg aaaaagcgat ggaaacaacc tttggtaaaa aacccatacc tacaaggggt
      781 ggtggcagta ttcctattgt agccttattt gaaaaagaat tgggtttaaa aagtgtattg
      841 atgggatttg gattagatag tgacttaata cattcgccta atgagcattt tggaattttt
      901 aattttctga aaggtattga aaccattcct cacttctttc ataactacac agcaataaaa
      961 acgaaataag catatcagga atattcagat taaaggggca ttacttatat gaaagaagtt
     1021 gaaaacaaga cttcaatttt ttatttaccc aaacaaagag gctgcctaaa ttttggcagc
     1081 ctttattatt tgaaggataa agaagtaact tcaattttac gggctaaagc tgcttcgggg
     1141 ctatatactg attgtgcttt gttgcgcaca tcatcactaa cttttactac atcagcctcg
     1201 cccattgctt ctttaacaat gattagctta ccgccatcaa aatagctttg caaaatgttt
     1261 tgattgtatt tattcaaata gttaataaag caactgattc ttcgttgtga taaacgaaag
     1321 ttatattgac tatcagctaa cggactcgac ataccacgta ccgttattgt aaccgtttta
     1381 tcgcgttgca aaagagataa aacatattga cacatctttt caagccgttg atatccgtct
     1441 aataaatcat cttcaaaaaa agtatgcatt cttaatgctg ctttttctct ttcatttact
     1501 gcaaagccaa gtgcgtaatt ttctttgtag gtacttgcgg ttaattggta cttcttaaag
     1561 agggcatcat aatcagcttt agttgtttct tttaaagttt ttgggtcggg ttcatcattg
     1621 tcaaaatata atcgtaaagg gaaagaaaat tctaattcat tttcaatgat acttcgtgtt
     1681 aaactatcgc gtgtgctaac cggaccctca tctaatgtta taacaccata acttttttgt
     1741 acttcgcctt ttattttttg tttttcgagc cggttaattt tgtaggcata caaatcataa
     1801 caacaggttt cggaattgat atgcaatgcg ccttttctgt tggtagttaa tatgccgctt
     1861 gtatcgttat catttacggt aaaatataaa tcattataac tgctgtttaa aggtaagcct
     1921 aaatttgtag ctgttttaaa ctgccccgtt aagttatccg atctaaaaac atcaaaaccg
     1981 ccaaagccat aatgaccttc gctactaaag tacaatgctt cttcagtttt tgcataaaag
     2041 ggtgtttcgt catcagcaaa agtgtttatg gcatctcctg cattaatggg tgttgtaaat
     2101 tgtccctgta aatccaattg catctgccaa atatccattt tgccataacc accgggcatg
     2161 ttactaacga agtgaattaa atatcctttg ttaccattgg tggtaatatt gggttgtgtg
     2221 taagtataag cctcgttaaa tgaactgaag tttacagg
//
LOCUS       DAOMAA010000307         2247 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_45335_length_2247_cov_2.558850, whole genome shotgun sequence.
ACCESSION   DAOMAA010000307 DAOMAA010000000
VERSION     DAOMAA010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45335_length_2247_cov_2.558850"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="PLO59_04920"
     CDS             complement(<1..274)
                     /locus_tag="PLO59_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadherin"
                     /protein_id="HQV00480.1"
                     /translation="MKAKLKLILTMVVTTISARAQTPNIVLTNLGSGLSEPVAVVNAG
                     DNRLFVVEQGGLIKVYKNGAFLATPFLNLTSVVTSTGNEQGLLGMAF"
     gene            583..1956
                     /locus_tag="PLO59_04925"
     CDS             583..1956
                     /locus_tag="PLO59_04925"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HQV00481.1"
                     /translation="MQKFKSLLVAAGIALVAQNSEAQKLPDNFFMTKLDNGMEVLVIE
                     DASVPLATVEIVVRNGAYTEDAQYNGLSHLYEHMFFKANKALPSQEQFLARVNELGIV
                     FNGTTSDERVNYFFTLSNSKVNEGLEFMNNAIRYPLFLETEMKKENPVVDGEFQRNES
                     NPYFHLFDAMNHRMWGDNYSRKNAIGDHDIILTATPEKMRVIQDKYYWPNNSMLVIAG
                     DVQHTAMFDKVKSIFGDWKPSTFNPFEKWPIPEFKPLEGTTYFTTENDNAKAPFILVG
                     YHGPDTRNDLPATYAADVFSFILGLQSSKFQKALVDGGLAYQAAIGYQTCKYTGPINV
                     QLVPNPMKMKEAYQELQKQISLFDTDDYFTDEQLEMAKNQLAIQDTYSKEKTSDFVHT
                     VTFWWASASINYYTDYVANLKKVTRADIKKYVQQYIKGKPSVTGLLISPQMKAMAKVD
                     SMETLAK"
     gene            1982..>2247
                     /locus_tag="PLO59_04930"
     CDS             1982..>2247
                     /locus_tag="PLO59_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00482.1"
                     /translation="MTNIIKQLSMLGLASCLTLTTFAQGQKTVTFDVAGLKVILRPTP
                     KDVISARLFVKGGTANYAKDKEGIEALAFSVAMDGGTTKMNNEV"
BASE COUNT          774 a          404 c          402 g          667 t
ORIGIN      
        1 taaaagccat acccaataag ccttgttcat tgcctgtact ggttacaaca gatgtgagat
       61 ttaaaaaagg tgttgctaaa aaagcaccgt ttttataaac ttttattaat ccgccttgct
      121 ctacaacaaa caaacggtta tcgcctgcat taacaacggc aacaggttcg cttaagccac
      181 tgcctaaatt agttagtacg atattaggtg tttgtgccct tgccgaaatg gttgttacaa
      241 ccatagtcag tatcaattta agttttgctt tcatgcgatg ctgttctatg ttaaatttaa
      301 tggatgccta ttgtttttga aaaatgtaag aacaacaaat tttgtttttt accatacacc
      361 attagtctaa aaattacaaa acaaaacatt aacatcaact tgtaataatt tgcatctgtt
      421 ttatttcgaa agcgattaac aagggctttc attcacaact aaatcataac tttttaaaat
      481 gtaacatcgt tcgatttaat atatctactg ttaaaataat tttaaacgtc ctatttccat
      541 ttaatattaa tttcattcgc aacaacatca aaaactaaaa acatgcaaaa atttaaatcc
      601 ttacttgtag ctgcaggtat tgcgcttgtt gcgcaaaaca gcgaggcgca aaaactaccc
      661 gataactttt ttatgaccaa actcgacaac ggtatggaag tattggttat cgaagatgca
      721 tcggtgccat tagcaacagt agaaattgtg gtacgtaacg gtgcttatac agaagatgca
      781 cagtataacg gactttcgca tttatacgaa cacatgtttt ttaaggccaa taaagcattg
      841 ccatctcaag agcagttttt agcccgcgta aatgaattgg gcattgtgtt taacggtact
      901 accagcgatg aacgtgtaaa ttactttttt acactgagca atagcaaggt taacgaaggt
      961 ttggagttta tgaataatgc catcaggtat cccttgtttt tagaaacgga aatgaaaaaa
     1021 gaaaatccgg tagtagatgg cgagtttcaa cgtaatgaat caaacccgta tttccatttg
     1081 tttgatgcca tgaaccaccg catgtggggc gataattatt cgcgcaaaaa tgcaattggc
     1141 gatcatgata ttatacttac ggctacgcct gaaaaaatgc gtgttataca ggataaatat
     1201 tattggccta ataacagtat gttggttatt gccggtgatg tacaacatac agccatgttt
     1261 gataaagtaa agtcaatttt tggcgattgg aaacccagta cttttaaccc attcgaaaaa
     1321 tggcccatac ctgaatttaa accattagaa ggcactactt attttactac tgaaaacgat
     1381 aatgccaagg caccatttat tttagtgggt tatcacggcc ccgatacacg taatgattta
     1441 ccggccacct atgctgccga tgtgttttca tttattttgg gattacaatc atcaaaattt
     1501 cagaaagctt tggttgatgg aggtttggcc tatcaggcag ctattggtta tcaaacttgt
     1561 aaatacacag gccctatcaa tgtacaattg gtaccaaatc cgatgaaaat gaaagaagcc
     1621 tatcaggaat tgcaaaaaca aataagttta ttcgataccg atgattattt taccgatgag
     1681 caattagaaa tggcaaaaaa tcaactggct atacaagata cttacagcaa agaaaaaacc
     1741 agcgattttg tacacacagt aactttttgg tgggcttcgg ccagcattaa ttattatacg
     1801 gattatgtgg caaaccttaa aaaagttacc cgtgccgata ttaaaaaata tgtacagcaa
     1861 tacataaaag gcaaaccatc ggtaaccggt ttgcttattt caccacaaat gaaagcaatg
     1921 gctaaagttg attcaatgga aacattggct aagtaattac caacatataa aaccactcac
     1981 aatgacaaat attataaaac aactcagtat gttagggtta gcaagttgct taacattaac
     2041 aacatttgca caaggccaaa aaaccgtaac ctttgatgta gcaggtttaa aagtgatatt
     2101 aaggccaaca cctaaagatg ttattagcgc ccgcttattt gtaaaaggcg gtactgcaaa
     2161 ttatgccaaa gataaagaag gcatagaagc cttggcattt agcgtagcta tggatggtgg
     2221 caccactaaa atgaataatg aagtttt
//
LOCUS       DAOMAA010000308         2245 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_45403_length_2245_cov_2.755251, whole genome shotgun sequence.
ACCESSION   DAOMAA010000308 DAOMAA010000000
VERSION     DAOMAA010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45403_length_2245_cov_2.755251"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PLO59_04935"
     CDS             complement(<1..233)
                     /locus_tag="PLO59_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HQV00483.1"
                     /translation="MIVEIFIPCFLDQFYPQTAMNMVKILEKVGCGVNYNPNQTCCGK
                     PALLAGYTDYCKEVGAKFIREFENDRPIVTPDAS"
     gene            complement(260..1582)
                     /locus_tag="PLO59_04940"
     CDS             complement(260..1582)
                     /locus_tag="PLO59_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014772476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQV00484.1"
                     /translation="MAKDTNVVSYKPKVVRSWIMYDWANSVYNLVITTAIFPIYYTSV
                     TKASANGLVNFYGFEIKNTVLYAYALSASFLVIAFISPLLSGIADNRGSKKFFMKVFC
                     FIGALSCMALFFFNSVQTVSIGITAFMLATMGYAGSIVFYNAFLPEIAPQNMHDKISA
                     QGYAMGYIGSSLLLIINLVMIQKPQWFGITDAGLASRIAFVTVGVWWIGFALITFKGL
                     HETPKNRNTNNYIQQGYVALYHVWQQLKTEPILRRFLSAFFFYNMGVQTVMYVASLFG
                     ANEIKMTESLLIATLLVIQFVAVIGALLFAWFSKLLGNINALCLATFVWIAICVSAYF
                     IYNATPFIILAGVVGLVMGGIQALSRATYAKLIKSKPEHASYFSFYDICDKAGMVLGT
                     LSFGLIEAISGSMRNSIFALAAFFIIGIILLRPLQKLKTKSIFKTNGV"
     gene            complement(1589..1993)
                     /gene="mce"
                     /locus_tag="PLO59_04945"
     CDS             complement(1589..1993)
                     /gene="mce"
                     /locus_tag="PLO59_04945"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015027316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HQV00485.1"
                     /translation="MLTKIEHIGIAVKNLENSNALFGKIFNAAPYKTETVESELVNTS
                     FFKIGSNKIELLAATNPQSAIASFIEKRGEGIHHIAFDVADIHAEMQRLKAEGFILLN
                     ETPKRGADNKWVCFLHPKSTNGVLIELCQQID"
BASE COUNT          712 a          438 c          399 g          696 t
ORIGIN      
        1 gatgcatcag gggtaacaat aggcctgtcg ttttcaaatt cgcgaataaa ctttgcgcca
       61 acttctttac agtaatcggt atagccggca agcagtgccg gtttgccaca gcatgtttga
      121 ttgggattat agtttacacc acaacctact ttttctaaaa tttttaccat gttcattgcc
      181 gtttgcggat aaaactggtc taaaaaacaa gggataaaaa tttcaactat cataactgaa
      241 acaggataac agccataggc tatacaccat tggttttaaa aattgatttc gttttaagct
      301 tttgtaaagg gcgcaaaagt ataattccga ttataaaaaa agcagccaat gcaaaaatgg
      361 aattgcgcat gctgccgctt atggcttcta tcaaaccaaa acttagcgtg cccaaaacca
      421 ttccggcttt atcgcatata tcataaaaac taaaatacga tgcatgttcg ggctttgatt
      481 ttataagttt tgcataagtg gcacgcgata atgcttgtat gccacccatt accaaaccaa
      541 ctacacctgc caaaataata aacggtgtgg cattataaat aaaataagcc gacacgcaaa
      601 tagcaatcca tacaaacgtt gccaggcaca atgcatttat attgcctaaa agtttactaa
      661 accatgcaaa cagcaatgcg cctattaccg caacaaattg tataactaac aaggttgcta
      721 ttaacaacga ttcagtcatt ttaatttcgt tggcaccaaa aagcgatgct acatacatta
      781 ccgtttgcac acccatatta taaaagaaaa atgccgataa aaacctgcgt aatatgggct
      841 cggtttttag ttgctgccaa acatggtaca atgccacata gccttgttgt atgtaattat
      901 ttgtgttacg gtttttgggc gtttcgtgca aacctttaaa agtaattaat gcaaaaccaa
      961 tccaccatac accaaccgtt acaaatgcaa tgcgcgatgc caaacccgca tcggtaatac
     1021 caaaccactg cggtttttgt atcataacta agtttataat aagcaaaagc gatgaaccaa
     1081 tgtaacccat tgcataaccc tgtgcactta ttttatcgtg catgttttgt ggggcaattt
     1141 cgggcagaaa agcattgtaa aaaacgatgc tgcctgcata tcccatagtt gccaacataa
     1201 aagctgttat acctatgctt accgtttgaa ccgaattaaa aaaaaataaa gccatgcatg
     1261 acaaagcacc tatgaagcaa aacactttca taaaaaactt tttactgcca cggttatctg
     1321 ctataccact aagtaatggc gatataaatg caattactaa aaaggacgct gacagtgcat
     1381 aagcatacag cactgtattt ttaatttcga aaccatagaa atttaccaaa ccattggccg
     1441 aggctttggt tacacttgta tagtaaatcg gaaaaatggc tgtggttata accaggttat
     1501 atacggagtt tgcccaatcg tacataatcc acgaccgaac cactttaggt ttatagctta
     1561 caacattggt atcttttgcc atacctgttt agtctatttg ctggcacaat tcaataagta
     1621 caccattggt agatttgggg tgtaaaaagc aaacccattt attatcggcc ccacgtttgg
     1681 gggtttcgtt aagcaatata aaaccttcgg cttttaagcg ttgcatttca gcatgtatat
     1741 ctgctacatc aaaagcaatg tgatgaatac cctcgcctct cttttcaata aatgaagcaa
     1801 tggcgctttg cggatttgtt gctgctaaca attcgatttt attagaacct atcttaaaaa
     1861 acgatgtgtt tactaattcg ctttcaacgg tttcggtttt atacggagct gcgttaaaaa
     1921 ttttgccaaa cagggcatta ctgttttcaa ggttcttaac ggctatgcca atgtgttcta
     1981 ttttggtaag catgttgttt tgctgtgatt tgttaaataa tgagcaaagt taggcttatt
     2041 acggcatata tagggtgtta aggcttcttt ttttgaaaaa aaaagcacat caagaatgtt
     2101 taacttcttg atgtgctttt ttatgggttg tacttggtag gttgtttgct gatagacaat
     2161 aaaaaaagct attgaccggt ttattttaat ttaatgtcgt ctattctcct ttcggcttct
     2221 tttatgtata tgtttgcttc ctctg
//
LOCUS       DAOMAA010000309         2244 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_45439_length_2244_cov_3.319781, whole genome shotgun sequence.
ACCESSION   DAOMAA010000309 DAOMAA010000000
VERSION     DAOMAA010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45439_length_2244_cov_3.319781"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="PLO59_04950"
     CDS             <1..555
                     /locus_tag="PLO59_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQV00486.1"
                     /translation="IELTGLTPESHKKIGALSKGYRQRVGLAQAMIHDPKVLILDEPT
                     SGLDPNQLADIRQLIKNIGKEKTVILSTHIMQEVTAICNRVIIINKGKIVADEAAHAL
                     VKQSNKPILIIEFGQSVSPALLKNIEGVLQVETLPNNTFRIHAQTDVRQALFTFAVNT
                     KNTVLSMQQENTNLENVFRQLTKN"
     gene            709..1968
                     /gene="metK"
                     /locus_tag="PLO59_04955"
     CDS             709..1968
                     /gene="metK"
                     /locus_tag="PLO59_04955"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016419764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HQV00487.1"
                     /translation="MPYLFTSESVSEGHPDKIADQISDALIDNFLAYDAASKVACETL
                     VTTGQVVLAGEVKSQAYLDVQDIARDVIKKIGYTKSEYMFEANSCGIISAIHEQSPDI
                     NQGVERKKPEEQGAGDQGMMFGYACRETDTYMPLPLELSHLLLRELAALRRENKAITY
                     LRPDAKSQVTIEYSDDNQPIRIDAIVISTQHDDFGKDAVMLKKIKEDVINILVPRMLK
                     KLPKRVQALFNDKIKYHVNPTGKFVIGGPHGDTGLTGRKIIVDTYGGKGAHGGGAFSG
                     KDPSKVDRSAAYATRHIAKHLVAAGVCNEALVQVAYAIGVAKPVGLFVNTYGTAQVAL
                     TDGQIADVILKMPEFDLRPYFIEKRFKLRTPIYLETAAYGHMGRECKVVKKTFNKGKA
                     NEKTVEVKLFPWEELNALAATKKAFKL"
BASE COUNT          757 a          457 c          414 g          616 t
ORIGIN      
        1 attgagttaa caggcttaac acctgaaagc cataaaaaaa ttggcgcact cagtaaaggt
       61 taccgccaaa gagttggctt ggcacaagcc atgatacatg acccaaaagt gttgatttta
      121 gacgaaccca catcgggcct cgacccaaac cagttagccg atatcagaca attgataaaa
      181 aatattggca aagaaaaaac ggttatactc tctacacaca ttatgcaaga agttacagcc
      241 atttgcaacc gtgtaattat tattaataaa ggcaaaatag tagccgatga agccgcgcat
      301 gcattagtga aacaaagtaa caaacccata ttaataatcg aatttggcca atctgtttcg
      361 cccgcattat taaaaaatat tgaaggtgta ttgcaggtag aaacgctgcc taataataca
      421 tttcgcatac atgcacaaac cgatgtaagg caggcccttt ttacttttgc tgttaataca
      481 aagaacaccg tattaagcat gcaacaggaa aataccaatc tcgaaaatgt tttcaggcaa
      541 cttactaaaa actaaagtac ggctctgttt actgtcgctt ttatgcatca tcgcaacaag
      601 cttttttaga gttattgcct aaaaatttta tgtttgcgcc tccaaaaaaa caggcacttt
      661 cagtcagcct gtatcaacat caaaacaaac actcaatatt tttttaaaat gccatactta
      721 tttacatctg aatcggtttc cgaaggccat cccgataaaa tagccgatca aatttctgat
      781 gctttaattg ataatttttt agcttatgat gctgcatcta aagtagcctg cgaaaccctg
      841 gtaacaaccg gacaggtagt tttagccggt gaggtaaaat cgcaagcgta tttggatgtg
      901 caggatattg cacgcgatgt gattaaaaaa attggttata ccaaaagcga atatatgttc
      961 gaagcaaact cttgcggtat tatatcggcc atacatgaac agtcgcccga cattaatcag
     1021 ggtgtagagc gcaaaaaacc tgaagagcaa ggtgccggtg accaaggaat gatgtttggt
     1081 tacgcatgcc gcgaaaccga tacctacatg cccttacctt tagagctttc gcatttatta
     1141 ttacgcgagt tagccgcatt acgcagagaa aacaaagcca taacctattt gcgacccgat
     1201 gccaaaagtc aggtaactat cgaatacagt gacgataatc aacccatacg cattgatgcc
     1261 attgtaatta gcacccagca tgacgacttt ggaaaggatg ccgtaatgct taaaaaaatt
     1321 aaagaagacg ttattaatat tttagtgcca cgcatgctta aaaaattgcc caagcgcgta
     1381 caggcattat ttaatgataa aattaaatac catgtaaacc caactggcaa gtttgtaatt
     1441 ggcgggccac atggcgatac tggtcttacc ggccgtaaaa ttattgtaga tacctatggc
     1501 ggaaaaggtg cacatggtgg tggtgctttt agtggtaaag acccatcaaa ggttgaccgc
     1561 agtgctgctt atgccacgcg ccatattgcc aaacatttag tggcagcagg tgtttgtaac
     1621 gaagccttgg tacaagtagc ctacgctatt ggtgttgcca aacctgtagg cctatttgta
     1681 aatacctatg gcaccgcaca agtagcactt accgatgggc aaattgccga tgttatttta
     1741 aaaatgcctg aatttgattt acgcccctac tttattgaaa aacgttttaa gctacgtaca
     1801 cccatttacc tcgaaaccgc agcctatggc cacatggggc gcgaatgtaa agtggttaaa
     1861 aaaacattta ataaaggtaa ggctaatgaa aaaacagtag aagttaaact gtttccttgg
     1921 gaagagttaa atgcattagc agctaccaaa aaagcattta aattataaac tgaaactaaa
     1981 aaatgccgcc ttaaacaagc ggcatttttt ttacaatgct cctgcttatt atttttaata
     2041 tagaacccac ctattcaaaa cttctgtctt caattatgcc cctttcaacg ctcaactcca
     2101 tttctatatt taccacttcc aaaacaagtt tcagttaacc ctttaattta ttctattgcg
     2161 aaaaatttat cagttatgaa aaaatcacgc acacaacaaa aattaggttt gtgaaaataa
     2221 attttcatta gagatggata tgca
//
LOCUS       DAOMAA010000310         2229 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_46041_length_2229_cov_2.953542, whole genome shotgun sequence.
ACCESSION   DAOMAA010000310 DAOMAA010000000
VERSION     DAOMAA010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46041_length_2229_cov_2.953542"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /gene="gcvT"
                     /locus_tag="PLO59_04960"
     CDS             complement(<1..708)
                     /gene="gcvT"
                     /locus_tag="PLO59_04960"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HQV00488.1"
                     /translation="MTTEVKETALTQKHISLGAKMAPFAGYNMPIQYEGLTIEHHNVR
                     NAVGMFDVSHMGEFILKGQHALDLIQRTTSNDASKLTAGKVQYSCLPNATGGIVDDLL
                     VYCIDEQTYMLVVNAGNIQKDWEWLMQNNTQGVEMHNISDKTSLLAVQGPKAINTLQK
                     LTDLPLADMPYYTFMKGVFCGIPNVLISNTGYTGAGGFEVYFENQYALTFWDAIMQAG
                     AAFNIKPAGLGCRDTLRL"
     gene            complement(740..1825)
                     /locus_tag="PLO59_04965"
     CDS             complement(740..1825)
                     /locus_tag="PLO59_04965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQV00489.1"
                     /translation="MPSILFVVPHRSNRAPSQRFRFEQYIRIWQQAGYRCTMAPLISE
                     HTDTIFYHPSSLFRKALLFANLLFIRLKHVIQSSAYDIVFIQREAFMTGTTFFEKQFK
                     RSGAKIIYDFDDAIWHHDVSEANKKFALLKRPQKTAQIIAMSDLVIAGNYYLKQYALQ
                     YNQQVLIIPTTIDTNNYIKKAVPRTKSGICIGWSGSVTTIKHFILAVPVLERLKKHCG
                     HNIYFKVIGDDTYLNEALEIQGIKWNEATEIDDLSEIDIGIKPLPNDEWSKGKCGLKG
                     LQYMALEIPTVMAAVGVNTEIIVEGVNGYLAEDENMWFEKILALINQPQLRQKMGKAA
                     RQTVIEKYSIHSQQQNYLNCTTQILSS"
     gene            1984..>2229
                     /locus_tag="PLO59_04970"
     CDS             1984..>2229
                     /locus_tag="PLO59_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HQV00490.1"
                     /translation="MNLFRKKSVDNILADAAKGYSDGEHSLGKHLGVRDLTAFGIAAI
                     IGAGIFSTIGNASANGGPGVVFLFIFTAIACGFSAFAY"
BASE COUNT          637 a          437 c          412 g          743 t
ORIGIN      
        1 gaggcgcaac gtatctctgc atcctaagcc tgcaggtttg atattaaacg ctgcaccggc
       61 ttgcataatg gcatcccaaa aagtaagtgc atactggttt tcgaaataca cttcgaaacc
      121 acctgcaccg gtgtaacctg tgtttgatat aagtacattt ggaatgccac aaaaaacgcc
      181 tttcataaaa gtatagtaag gcatatcggc taacggtaaa tcggttagtt tttgtaaggt
      241 attaatagct ttaggacctt gtactgcaag caacgatgtt ttgtctgaaa tgttatgcat
      301 ctcaacacct tgtgtattgt tttgcataag ccactcccaa tctttttgaa tattgcctgc
      361 attaactacc agcatatacg tttgttcatc aatacagtaa accagcaaat cgtcaacaat
      421 acctcctgta gcattgggca aacaagagta ctgcacttta cctgctgtta gttttgatgc
      481 atcgttactg gtagttcgtt gaattaaatc taaagcatgc tggcctttta aaataaattc
      541 gcccatgtgc gatacatcaa acataccaac agcattgcgt acgttatgat gttcgatggt
      601 taatccctca tattgaatgg gcatattgta gcctgcaaaa ggtgccattt ttgcacctaa
      661 tgaaatatgt ttttgtgtaa gtgccgtttc tttaacctct gtggtcataa ataaaaatgt
      721 tttaaatttt aagtgtgttt tatgaagata atatttgtgt tgtgcagttt aaataatttt
      781 gctgctgcga atgtatagaa tacttttcta tgactgtttg ccttgctgcc ttacccattt
      841 tttgccgcaa ctgtggttga ttaataaggg ccagtatttt ttcgaaccac atgttttcgt
      901 cttcggctaa gtaaccattt acaccctcaa caattatttc ggtatttaca cctacagcag
      961 ccataacggt aggtatttct aatgccatgt attgcaagcc ttttaagcca catttaccct
     1021 tactccattc atcgtttggc aaaggcttta taccaatatc aatttcgctt aagtcgtcaa
     1081 tttcagtggc ttcattccat ttaatgccct gtatttccaa agcctcattt aagtatgtat
     1141 catctccaat tactttaaaa taaatgttat ggccacaatg tttttttaag cgttccaaaa
     1201 ccggtacagc caatataaaa tgcttaatag tagttacgct tccactccag ccaatacata
     1261 ttccggactt ggtacgaggt acagctttct ttatataatt atttgtgtcg atggttgttg
     1321 gtattatcaa cacttgctga ttgtattgca gggcatattg tttcaaataa taattaccgg
     1381 caataaccaa atcgctcatg gcaattatct gagctgtttt ttgaggacgt ttaagcaagg
     1441 caaacttctt attggcttcg cttacatcat gatgccaaat tgcatcatca aaatcgtaaa
     1501 ttattttagc gccacttcgt ttaaattgct tttcgaaaaa ggtagtgcct gtcataaacg
     1561 cttcgcgttg aataaaaaca atatcataag ccgatgattg tattacatgc tttaacctga
     1621 taaataataa attggcaaac aataatgctt tccgaaaaag gctgctgggg tgataaaaaa
     1681 ttgtatcggt atgttcgctg attaacgggg ccattgtgca acggtaaccg gcttgctgcc
     1741 atatacggat atattgctcg aacctgaacc gctggctggg tgctcggttg ctgcgatgcg
     1801 gtaccacaaa caatatgctg ggcataatag attttaaaat gggatgcgaa gttggaaatt
     1861 attgccaaat atttatccac tcgaagcagg tacacctaca ctatattttt tacagtttct
     1921 aaatcaagct aacctgcatt ttttaccttg ctgcacttaa aaaataatac tacactaaag
     1981 cctatgaatt tattcagaaa aaaatcagtg gataatatac ttgccgatgc cgccaaaggc
     2041 tatagcgatg gcgaacatag tttgggtaaa cacttaggcg tgcgcgactt aactgccttt
     2101 ggtatagctg ctattattgg tgccggcata ttttctacca taggtaatgc cagtgcaaat
     2161 ggcggccctg gtgtagtttt cttatttatt tttactgcta ttgcatgtgg ttttagtgcc
     2221 tttgcttat
//
LOCUS       DAOMAA010000311         2229 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_46051_length_2229_cov_2.414903, whole genome shotgun sequence.
ACCESSION   DAOMAA010000311 DAOMAA010000000
VERSION     DAOMAA010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46051_length_2229_cov_2.414903"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="PLO59_04975"
     CDS             complement(<1..548)
                     /locus_tag="PLO59_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HQV00491.1"
                     /translation="MAILTFQKPDKVVMNHSSETVGEFEFRPLEPGYGVTIGNALRRI
                     LLSSLEGYAITSIKVAGVDHEFSTIKGVVEDVIEMVLNLKQVRFKRQIEGTTFEKVTV
                     VVSGKEQFTAGDIAKHTTAFQVLNPELVICNMEQSVKLQLDITIDKGRGYVPAEENKN
                     ANQLQGTIPIDSIYTPIKNVKY"
     gene            complement(610..1215)
                     /gene="rpsD"
                     /locus_tag="PLO59_04980"
     CDS             complement(610..1215)
                     /gene="rpsD"
                     /locus_tag="PLO59_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015753976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HQV00492.1"
                     /translation="MARYTGPKTKIARKFREAIFGPDKYFDKRSYPPGQHGVNKKRAK
                     QSEYAVQLSEKQKVKYTYGILERQFAKTFDRASRMQGITGENLLKLIESRLDNVVYRM
                     GIAPSRAAARQLVGHCHIQVNETVVNIPSFTVRAGDIISVRERSKSLEVITDSLKSRS
                     NKFSWIEWDGALMTGKFIATPERSEIPENIKEQLIVELYSK"
     gene            complement(1271..1672)
                     /gene="rpsK"
                     /locus_tag="PLO59_04985"
     CDS             complement(1271..1672)
                     /gene="rpsK"
                     /locus_tag="PLO59_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HQV00493.1"
                     /translation="MATQKTAASKTVKKKVVKVEATGVAHVSATFNNIIISLTNLSGQ
                     VISWSSAGKMGFKGSKKNTPYAAQMAASDCAKVAYDLGLRKVKVIVKGPGAGRESAIR
                     TINAAGIEVSEILDVTPIPHNGCRPPGRRRI"
     gene            complement(1747..2124)
                     /gene="rpsM"
                     /locus_tag="PLO59_04990"
     CDS             complement(1747..2124)
                     /gene="rpsM"
                     /locus_tag="PLO59_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011586488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HQV00494.1"
                     /translation="MARISGIDLPKHKRGEIGLTYIYGIGRSTAQYILGKAGIPVDKK
                     VDEWNDKELTDIRTIINDEIKVEGSLRSEVQLNIKRLMDVGSYRGIRHRSGLPVRGQR
                     TKNNCRTRKGKRKTVANKKKVTK"
     gene            complement(2162..>2229)
                     /gene="rpmJ"
                     /locus_tag="PLO59_04995"
     CDS             complement(2162..>2229)
                     /gene="rpmJ"
                     /locus_tag="PLO59_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HQV00495.1"
                     /translation="IKRKGKVMVICENPKHKQRQG"
BASE COUNT          588 a          457 c          393 g          791 t
ORIGIN      
        1 taatacttta catttttaat tggcgtgtaa atagaatcaa ttggaatagt gccttgtaac
       61 tgattggcat ttttgttttc ctcagccgga acataaccac gtcctttgtc aatggtaata
      121 tcaagctgaa gctttactga ttgctccata ttacagatta ccaattcagg attcaacacc
      181 tgaaatgcag ttgtatgttt ggcaatatca ccggccgtaa attgctcttt accacttact
      241 actacggtaa ctttttcgaa tgttgtgcct tcaatttgtc gtttaaatcg tacctgttta
      301 aggtttaaca ccatttcaat tacatcttca acaacacctt taatggtaga aaactcgtga
      361 tcaacaccgg caactttaat actggtaatt gcataacctt ctaatgaaga aagtaatata
      421 cgccttaatg catttccaat ggtaactccg tagccaggtt caagtggacg aaactcaaac
      481 tcaccaacag tttctgatga gtgattcatt acaactttgt ctggtttttg gaatgttaat
      541 atggccattt gtttttaatt taattttttg ttttagaatt atttcttttc aactaatcct
      601 tggctattat tacttagagt acaattcgac aataagttgt tctttaatgt tttcaggtat
      661 ttctgatcgc tcaggtgtag caataaattt accggtcata agggcaccat cccactctat
      721 ccatgaaaat ttgttgctgc ggctttttaa gctatctgta attacttcga gcgatttaga
      781 acgttcacgg acagaaatta tgtcacccgc tcttactgta aatgaaggga tatttactac
      841 cgtttcattt acctgaatgt ggcaatggcc tactaactga cgtgcagcag cacgcgaagg
      901 tgcaataccc atacggtaaa ctacattgtc taatctcgat tcgataagct ttaataggtt
      961 ttcgcctgta ataccttgca tacgtgatgc acgatcgaat gttttagcga actggcgctc
     1021 taaaatgcca taagtatatt ttactttttg tttttcagaa agctgcacag cgtattctga
     1081 ttgctttgcg cgctttttgt ttacaccgtg ttgtcccgga gggtaacttc ttttgtcaaa
     1141 atatttatcc ggaccgaaga tagcctcacg aaatttacga gcaattttag ttttagggcc
     1201 tgtataacgt gccattataa ttgttgtttt taatttttaa ttgattttta aatagcagaa
     1261 ttcgtattaa ttaaattctt ctacgacccg gagggcggca gccattgtgc ggaattggtg
     1321 ttacgtctaa tatttccgaa acttcgatac ctgctgcatt aatagtacgt attgctgatt
     1381 cgcgacctgc accgggtcct tttacgataa cctttacttt acgcaaacct aaatcatagg
     1441 ctaccttagc acaatcgctt gctgccattt gagctgcata tggtgtgttt ttctttgaac
     1501 ctttaaagcc catcttacct gcactcgacc atgatattac ctgacctgaa aggttggtta
     1561 aagaaataat tatattatta aaagttgcac taacatgggc tacgcctgtt gcctcaacct
     1621 ttacaacctt tttctttacc gttttacttg ctgctgtttt ttgtgttgcc attgcttttt
     1681 attaatatgc ttaaatcctt ggcgggatga ttaattgtaa attgatatta ctgttgcatt
     1741 taaagattac ttggtaacct ttttcttatt agcaactgtt ttgcgtttac ctttgcgggt
     1801 acggcaattg ttttttgtac gctggccgcg tactggtaaa ccactgcgat gtctgatacc
     1861 acgatacgaa ccaacatcca ttaaacgctt aatgtttaac tgaacttcgg agcgaagcga
     1921 cccttctacc tttatctcat cattaatgat ggttcggata tcggtcaatt ctttatcatt
     1981 ccattcatct acttttttat ctaccggaat gccagccttg cccaatatgt attgcgcagt
     2041 tgaacggccg atgccatata tataggttaa acctatttcg cctcttttat gttttggtaa
     2101 atctatacct gaaatacgag ccatgctttt gcacctccaa aatgttgtgc ctaaaggcta
     2161 attagccctg gcgctgcttg tgcttagggt tctcacagat aaccatgact ttacccttac
     2221 gcttgataa
//
LOCUS       DAOMAA010000312         2228 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_46089_length_2228_cov_2.364933, whole genome shotgun sequence.
ACCESSION   DAOMAA010000312 DAOMAA010000000
VERSION     DAOMAA010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46089_length_2228_cov_2.364933"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..1235)
                     /locus_tag="PLO59_05000"
     CDS             complement(105..1235)
                     /locus_tag="PLO59_05000"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004919840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HQV00496.1"
                     /translation="MQKIAMVDLKGQYQKIKPQVDAALAGVIENTAFINGPEVKNFQA
                     DFEKYLGIKHVIPCANGTDALQIAMMALGLQPGDEVITASFTYVATAEVIALLQLTPV
                     LVEVDAQTFTISPAAIEAAITPKTKAIVPVHLYGQCAPMEAIMEIAKKHNLFVIEDTA
                     QAIGADYKFANGTTQKAGTIGTIGCTSFFPSKNLGCYGDGGAIFTHDDTLAAKLRMMA
                     NHGQGKQYYHDEIGCNSRLDSLQAAVLRIKLPHLDSYAAARNQAATYYDKAFANNTKI
                     QTPVRAPWSTHVFHQYTLQLNGVNRDGLREYLASKEIPAMIYYPVAVHQQKAYRSSRY
                     PDGHFPLTEALTQCVISLPMHTELTQEQLAYITQHVLAYINQ"
     gene            1458..2204
                     /locus_tag="PLO59_05005"
     CDS             1458..2204
                     /locus_tag="PLO59_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00497.1"
                     /translation="MTKKILLLLFLISRQLMAQVSIKDSSINAVLVGMGAGLYTPAGD
                     MADRFGNNVNIHLSCALKRTNNWIFGIEGEYIFGNTIKEPNIFKNIQTNDGFIIGNDG
                     KFATVRTFERGYIATFNVSKVLPLLGPNKNSGFIIKAGVGFMQHKIKIDPIGNTVPYL
                     DKEHRKGYDRLSNGLALHQFIGYQYLGNTRAINFFGGFELFEGFTQSRRSYNHDTMIA
                     DTKKRTDILIGFKVGWILPLYKQAPSEFYY"
BASE COUNT          656 a          431 c          437 g          704 t
ORIGIN      
        1 attacccatt tcggcaaaac aagtaccggt aactaagccc acgtatccgg ttccaactac
       61 tgctatattc ataaaataat ttggtgttta attttaattt ttaattactg attaatgtat
      121 gctaatacat gttgtgttat gtatgccaat tgctcttgtg taagttcggt atgcatgggc
      181 aatgaaataa cacattgggt taatgcttcg gttaaaggga agtggccatc ggggtagcgg
      241 ctgcttcgat atgctttttg ctgatgtaca gctaccgggt aataaatcat agccggaatt
      301 tctttgctag ccaaatactc gcgcaaacca tcgcggttaa cgccatttaa ctgcaaagta
      361 tattgatgaa acacatgtgt actccatggt gcgcgcaccg gagtttgaat tttagtgtta
      421 ttggcaaaag ctttatcata ataggttgca gcttggttac gtgcggctgc atagctatct
      481 aaatggggta gttttatacg caacaccgca gcttgcaaac tatctaaccg cgaattgcag
      541 ccaatttcat catgataata ttgtttaccc tgaccatgat tggccatcat cctcagttta
      601 gctgccaagg tatcatcatg cgtaaaaatt gcacctccat cgccataaca acctaagttt
      661 tttgatggaa aaaacgatgt gcagcctatg gtaccaattg tgcctgcttt ttgtgttgta
      721 ccgttggcaa acttataatc ggcacctatg gcttgtgctg tatcttcaat cacaaacaag
      781 ttatgctttt tggcaatttc cattatcgct tccattggcg cgcattggcc atataaatga
      841 acgggtacaa ttgctttggt tttaggcgta atagctgcct ctatggctgc cgggctaatg
      901 gtaaaagttt gcgcatcaac ctctaccaac acaggtgtaa gttgcaataa agcaattact
      961 tcggcagtgg ctacataggt aaatgaagcc gtaattactt cgtcaccggg ctgcaaacct
     1021 aaagccatca tggcaatttg taaagcatcg gtaccgttgg cacaaggtat aacatgttta
     1081 atgcccaaat acttttcgaa atcggcctga aaatttttaa cctcggggcc gtttataaat
     1141 gcagtgtttt caattacacc ggccaaagca gcatctactt gcggttttat tttttgatat
     1201 tgacccttaa ggtcaaccat ggcaattttt tgcattttat tatttttatt tctgatgtgt
     1261 actgtttggc atcaaccggt atatcattta attattttat aacgataacc cttttgccaa
     1321 atgaaagcct aaacaccttt tttaagcgag cgcgaaagta attatttata tcaattggat
     1381 atggttttga aacgcttttg tatagcactt taataaacca caaaagcctt tatatttggc
     1441 tcccccattt atagtctatg acaaaaaaaa tactactgct tttatttctt atcagccggc
     1501 aattaatggc acaggttagt attaaagact cgtcaattaa tgccgtattg gtgggcatgg
     1561 gtgccggttt atatacacct gccggagata tggccgacag gtttggcaat aatgtaaaca
     1621 ttcatctgtc ttgtgcactg aaacgcacta ataactggat ttttggtatt gaaggcgaat
     1681 acatttttgg caatactatt aaggagccta acatttttaa aaacatccaa accaacgatg
     1741 gttttataat aggcaacgat ggtaaatttg ctaccgtacg cacatttgaa cgtggctata
     1801 ttgcaacatt taatgtgtcg aaagtgctgc cgcttttagg ccccaataaa aacagtggtt
     1861 ttattataaa agccggtgta ggttttatgc aacataaaat taagattgac ccaattggca
     1921 acaccgtgcc ttacctggat aaagaacacc gcaaagggta tgacagactt agtaatggat
     1981 tggccttaca tcagtttatc ggctatcaat atttgggaaa tacacgggcc attaattttt
     2041 ttggcggttt cgaactattt gaaggcttta cacaaagcag gcgcagttat aatcatgata
     2101 cgatgatagc cgacacaaaa aagcgaaccg atattttaat tggatttaag gtaggatgga
     2161 ttttgccttt gtataagcaa gcacccagcg aattttacta ttaatatcaa acataaaaac
     2221 taaacctt
//
LOCUS       DAOMAA010000313         2225 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_46200_length_2225_cov_2.713364, whole genome shotgun sequence.
ACCESSION   DAOMAA010000313 DAOMAA010000000
VERSION     DAOMAA010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46200_length_2225_cov_2.713364"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..383
                     /locus_tag="PLO59_05010"
     CDS             63..383
                     /locus_tag="PLO59_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00498.1"
                     /translation="MKNTLLVLSTLGCIIWSCSPKTAPTSSQTKPATEQAPDNSIPVS
                     VDMASAEKTYRSVCSGCHELYPTTNYTAAEWGGIVNRMQKKANFSNSDKNLIMAFLSA
                     NAKK"
     gene            complement(387..1100)
                     /gene="rnc"
                     /locus_tag="PLO59_05015"
     CDS             complement(387..1100)
                     /gene="rnc"
                     /locus_tag="PLO59_05015"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HQV00499.1"
                     /translation="MLAYLKIIKNALSAERQFFQSMYNLTGFYPRNLRLYKLAFVHKS
                     IMREQNQGKYLSNERLEYLGDAVLGAVVADLLFKKFPYRNEGFLTEMRSRIVSRDSLK
                     HVAIKMGIKAWVANIENSSSRSMYGDALEALIGAVYLDCGYKAAQQFVLNRIIQIHMD
                     LEEKETNDTNFKSRLINHAQKEKHELLFDMVQDEQAGRQFTISIKYNGAEIARATEYS
                     KKRAEQQAAAIACKALELL"
     gene            complement(1115..>2225)
                     /locus_tag="PLO59_05020"
     CDS             complement(1115..>2225)
                     /locus_tag="PLO59_05020"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HQV00500.1"
                     /translation="QQSGQAQAFSPSFMYNQISLTGCQGSYIQRAVEKMQQVGAIPLS
                     QFAYTDEDCSRKPNANELQAANTYRIKGFQRLSVAGDDYKTDLLAMKQYLAQGSPVII
                     GNMVGGSFMQNMLGKDTWIPTRDDYNENGFGGHCMCVIGYDDFKFGEEGGFQIMNSWG
                     PEWGNNGIAWVRYKDFEYFNKEAYAIYPMGNNQKFDNQKLAAGIGLIDNATKANISLI
                     RSGSVFKTAQAIPKGTKFKIEVNNSIECYVYVLGQDTDASSYILFPYTKKHSPYCGVT
                     GMRLFPRDYSLTADDYGNTDYMAIVITKTPIDFNKLNASVNASRQNTYEAKIKEALAG
                     TLIENVNYQPGNNLTFQCDVGNKNAVAVVVQIDKK"
BASE COUNT          648 a          486 c          390 g          701 t
ORIGIN      
        1 tgatttttcc catagttcaa ctttaagaca caatatttta gtgcaaataa aaacttcaac
       61 atatgaaaaa cactttactt gtattaagca cattaggttg tattatttgg agttgcagtc
      121 ctaaaactgc accgacctca tcgcaaacca aacccgcaac agaacaggca cccgataact
      181 ctataccggt ttctgttgat atggcttcgg ccgaaaaaac ataccgttcg gtatgcagtg
      241 gctgtcatga actttaccct accactaatt acacagcagc cgagtggggt ggcattgtta
      301 accgtatgca gaaaaaagca aactttagta acagtgataa aaatctgatt atggcatttt
      361 tatcagccaa tgcaaaaaaa taacagctat agcaattcaa gtgctttgca ggcaattgca
      421 gctgcttgtt gctcggctcg ttttttcgaa tattcagtgg cgcgggctat ttccgcgcca
      481 ttgtatttta tcgaaatggt aaactgacgg cctgcctgtt cgtcttgtac catatcaaaa
      541 agcaactcat gtttttcttt ctgtgcatgg tttattaaac ggcttttaaa attggtatcg
      601 tttgtttctt tctcttctaa atccatgtgt atttgtataa tacggttaag cacaaattgt
      661 tgcgctgcct tataaccaca atctaaatac acggcaccaa ttaaagcttc caatgcatcg
      721 ccatacatac tgcgcgaact gctgttttcg atattggcaa cccaggcttt tatgcccatt
      781 tttatggcca catgttttaa actatcgcga cttacgatac gcgatcgcat ttcggttaga
      841 aagccctcgt tgcgataagg aaatttttta aaaagtaaat cggctacaac agcacctaac
      901 acagcatcgc ctaaatactc aagcctttca ttactcaaat acttaccttg attttgttcg
      961 cgcattattg atttgtgtac aaaagccaat ttatacaaac gcaaattacg cgggtaaaaa
     1021 cccgttaaat tatacattga ctgaaaaaat tgcctttcgg ccgacaatgc attttttata
     1081 atttttaaat aggctaacac aggctgctgc gtttttattt tttatcaatc tgaaccacaa
     1141 cagccacagc atttttattg ccaacatcac actgaaacgt taggttattg cccggctgat
     1201 aattaacgtt ttcgattaaa gtacccgcaa gcgcctcttt aatttttgct tcataggtgt
     1261 tttgcctgct tgcattaact gatgcattaa gtttattaaa atcaattggc gttttggtaa
     1321 taacaattgc catgtaatct gtatttccat agtcgtctgc tgttaaacta taatcgcgtg
     1381 gaaacaaacg catacccgta acaccacaat agggcgagtg ttttttggtg tatggaaaaa
     1441 gtatatagct acttgcatcg gtatcctgac ccaatacata cacataacac tcaatactgt
     1501 tatttacttc aattttaaac ttagtgcctt taggtatagc ctgagcggtt ttaaaaacag
     1561 acccactgcg tataaggctt atgttagctt tggtagcatt atcaataagg ccaatgcccg
     1621 ctgccaattt ctgattgtca aatttttgat tgttgcccat ggggtatatg gcataggctt
     1681 ctttattaaa gtattcgaag tctttatagc gcacccaggc gataccgtta ttaccccact
     1741 cagggcccca tgaattcatt atctgaaatc caccctcttc cccaaattta aaatcatcat
     1801 aacctataac acacatacaa tggccgccaa atccattttc attataatca tcgcgtgtag
     1861 gaatccaggt gtctttgcct aacatgtttt gcataaaact gccaccaacc atattaccaa
     1921 taattactgg cgaaccttgc gccaggtatt gtttcatggc aagcaaatcg gttttgtaat
     1981 catcgccagc aactgataac cgctgaaaac cctttatacg atatgtgtta gctgcctgca
     2041 attcgtttgc attgggttta cggctgcaat cttcatcggt atatgcaaat tgcgaaagtg
     2101 gaatggcgcc tacttgctgc attttttcga cagcacgttg tatgtaagaa ccctgacaac
     2161 cggttaacga aatttgattg tacataaacg aaggactaaa agcctgtgcc tgcccgcttt
     2221 gttgt
//
LOCUS       DAOMAA010000314         2220 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_46392_length_2220_cov_2.139030, whole genome shotgun sequence.
ACCESSION   DAOMAA010000314 DAOMAA010000000
VERSION     DAOMAA010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46392_length_2220_cov_2.139030"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="PLO59_05025"
     CDS             <1..413
                     /locus_tag="PLO59_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00501.1"
                     /translation="FSIPQFEKHMQAGKKVYDFIEAQIKFLPVGIIPLHMSEGYIFLR
                     NGRETEMRVYNYQITIFESADEKYRSIHTTFVSSYHKHFANTYESIKRDIIKQHTEMP
                     NPATYVFESDLNIPLEPTYLPIVKRMLVKHVCSN"
     gene            418..1857
                     /locus_tag="PLO59_05030"
     CDS             418..1857
                     /locus_tag="PLO59_05030"
                     /inference="COORDINATES: protein motif:HMM:NF015836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00502.1"
                     /translation="MSITKVYKQPLFSWLILLALPVYILTVLNNIGYFYADEHYQIIE
                     FALHILGKVPDQLLAWEYKYGLRNGVLPLIASGLIYLCEVAGIDNPFSQALLLRAIGA
                     IVFVTTTIYVAVKNYSPANKTQTIGIMGLTWLLWFMPMLARFSSENFAACLLVILVTL
                     LENKKTRWYILTVTAVLICIRYQTIIVVLTIWLHSWLIVRKNVWPLIAGVFAALIVDV
                     IAGYFLYHKAICPFFNYALMTIGGTGPNFGEQPFYFYITNVCKLAGYPLGITIWIACA
                     YYIITKPKDLISWITVVFLLVHTVMPHKEIRFLFPLILLLPYMFRHLFYKAYFYLTKK
                     VFAFLIFTIAIFNLALLICNCFLPACSPVALINMINKQTMPQHLLYLPYCNPYNPFES
                     LPMQFYANSGVQKIKINQMADMALQTKSNCLLSITQTQYENNAGFLKAMGFKRYDSAY
                     PVMLVKYNCGIWLHGDNNLMLLKKSDYNK"
     gene            complement(1894..>2220)
                     /locus_tag="PLO59_05035"
     CDS             complement(1894..>2220)
                     /locus_tag="PLO59_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00503.1"
                     /translation="SFLSSLRFAADALVGFVAIRLFFGVIAFCRKFKNFSIAFVLFPS
                     WLRSVCDVIRKMPVAFIFDLAFILIASFYSVVNKTVCATLKQSVTLVATLFTFWPPAL
                     LLCEIS"
BASE COUNT          730 a          408 c          391 g          691 t
ORIGIN      
        1 atttttcgat acctcaattt gaaaaacata tgcaagccgg caaaaaagtt tacgacttta
       61 ttgaagcgca aattaaattt ttacccgtag gtattattcc gttgcacatg agcgaaggtt
      121 atattttttt acgcaatggc cgcgaaaccg aaatgcgtgt ttacaattat caaataacca
      181 ttttcgaatc ggctgacgaa aaatacagaa gcattcatac tacatttgtt agcagctacc
      241 acaagcattt tgcaaacacc tacgaaagca taaagcgtga tataataaaa cagcatactg
      301 aaatgcccaa tccggctacg tacgtatttg aatctgattt aaacataccc ttagaaccca
      361 cttacctgcc cattgtaaag cgtatgctgg taaagcatgt ttgcagtaat taaaagcatg
      421 tctattacca aggtttacaa acaaccactt tttagttggt taattttatt agcgctgccg
      481 gtttatattt taacggtgct aaacaacatc ggatattttt atgctgatga gcattaccaa
      541 attattgagt tcgcattgca catattgggc aaagtccccg accaattgtt agcatgggag
      601 tataagtatg gcttacgtaa cggggttctg cctttaatag catcgggcct gatatatttg
      661 tgtgaggtgg ccggtattga caacccattt agccaagctt tgttgcttag agccataggc
      721 gcaattgtat ttgtaactac tactatatat gtggctgtta aaaattacag ccctgccaac
      781 aaaacacaaa ccattggtat tatggggcta acgtggttgc tttggtttat gcccatgctt
      841 gcccgtttta gttccgaaaa ttttgccgca tgtctattgg ttattttagt cactttgctt
      901 gagaataaaa aaacaagatg gtatatatta actgtaacag cagtattaat ctgcattcgt
      961 tatcaaacaa taattgttgt gctaacaata tggttacaca gttggcttat cgtaagaaaa
     1021 aacgtttggc cactcatagc cggtgtgttt gccgctttga ttgtggatgt aattgcaggc
     1081 tattttttat atcacaaagc tatttgcccc ttttttaatt atgcattaat gactattggc
     1141 ggaaccggac caaattttgg cgaacaacct ttttactttt atataacaaa cgtttgtaaa
     1201 ctggccggat atcctttagg tataaccatt tggattgcat gcgcatatta tataattacc
     1261 aaacctaaag atttaatcag ttggattacg gttgtttttt tattggtaca tactgttatg
     1321 cctcataagg aaattcgatt tttatttcca ttaattttac tattaccgta tatgttccgg
     1381 catctctttt ataaagctta tttctattta acaaaaaaag tatttgcctt tttaattttc
     1441 accatcgcta tatttaatct ggcactttta atctgcaatt gttttttacc tgcatgcagc
     1501 ccggtagcat taataaatat gattaacaag caaaccatgc cgcaacattt gctatatctg
     1561 ccctactgca atccatacaa tccgtttgaa agtttgccta tgcaatttta tgcaaacagt
     1621 ggagtgcaaa aaattaaaat caatcaaatg gctgacatgg cgctgcaaac caaaagcaat
     1681 tgcttattaa gcattacaca aacacaatat gaaaacaatg ccggtttttt aaaagcaatg
     1741 ggttttaaac gctatgatag tgcttatccg gtaatgctgg taaaatacaa ttgtggtatt
     1801 tggttgcatg gcgataataa tttaatgcta cttaaaaagt cggattataa taaataaacc
     1861 tatggacagg catgcaatat taaaagaggt ttattacgaa atttcgcata gcagcagggc
     1921 cggtggccaa aacgtaaaca aggtagctac caaggttaca ctttgtttta atgtggcgca
     1981 aacagttttg tttacaacag aataaaaaga ggcaataaga ataaaagcca agtcgaagat
     2041 aaatgcaact ggcattttac gaatcacatc acaaacagaa cgcagccaac ttggaaacaa
     2101 aacaaaagct atcgaaaaat ttttaaactt tctacaaaag gctatcacac caaaaaacaa
     2161 acgtattgcc acaaaaccaa ccagggcatc ggcagcaaaa cgaagtgatg ataaaaagct
//
LOCUS       DAOMAA010000315         2217 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_46503_length_2217_cov_2.293247, whole genome shotgun sequence.
ACCESSION   DAOMAA010000315 DAOMAA010000000
VERSION     DAOMAA010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46503_length_2217_cov_2.293247"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="PLO59_05040"
     CDS             complement(<1..757)
                     /locus_tag="PLO59_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015889194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HQV00504.1"
                     /translation="MKLKLLSAIAFSSFFNLANAQQTDRCGTMQYLDQLIKDDPALLQ
                     RMEQQEQVLQTQLANENSSGTQAVVTIPVVVHVVYNTAAQNISDDMIKSQIDVLNEDF
                     SAKNSDKIKVPPYFQHLIGNPEIQFKLAARDPQGNATNGIVRVATTTASFSTNNAVKS
                     AATGGSNAWATTQYLNLWVCNMGGGILGYAQFPGTGSAATDGVVITYQSMGRVSAGSP
                     YNLGRTATHEVGHWLNLRHIWGDGGCGEDDHCED"
     gene            complement(886..>2217)
                     /locus_tag="PLO59_05045"
     CDS             complement(886..>2217)
                     /locus_tag="PLO59_05045"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HQV00505.1"
                     /translation="QNVFIKSFERKNLTYVVNEVDDKQLRALQILNSVTGSAIIYCRN
                     RRRTQETALYLNQHNITADFYHAGLTTMQRNNKQEDWIKNKIRVMVATNAFGMGIDKP
                     DVRLVIHIDMPDNLEAYYQEAGRAGRDEHLAFAVLLFNSNDIADMHQRVAQNFPVLDV
                     IRNVYSALCNYLQIATGFGLNDEFEFDIVNFCKVFKLDAYQVSAVLHILEMDKLIGLT
                     DGVFMPSRLHILLQNEDLYRFQMEHPELDVLIKTLLRSYGGLFDDFVQIHEGEIAQRC
                     KLEIPELINKLLLLQKYGVIEYLKQTDKPLLTFLHQRVPNTHLEISVSVLAARKKRYE
                     IRLKAMTQYAEEKHKCRSLMLLSYFNEEIKQRCGVCDYCRNRNKLNINEIEFEAIMQS
                     IKEHIAHGSFDDESLLKSLNTRHPEKALAVLNWMLDHELLKVDKQNNLALA"
BASE COUNT          633 a          452 c          406 g          726 t
ORIGIN      
        1 tatcttcgca atgatcatct tcgccgcagc ccccatcacc ccaaatatgg cgcaggttta
       61 accaatgccc tacttcatgt gtggccgtac ggcccaagtt gtaaggcgaa ccggccgaaa
      121 cacgccccat tgattgatac gtgataacca caccatcagt tgctgccgaa ccggtgcccg
      181 gaaattgtgc atatcccaaa ataccgcctc ccatattgca aacccaaagg tttaaatatt
      241 gtgtagttgc ccaggcgtta gaaccaccag ttgctgccga tttaactgca ttatttgttg
      301 agaatgatgc agtagttgta gctacgcgta caataccatt ggtagcatta ccttgcgggt
      361 cgcgtgctgc cagtttaaat tgaatttcgg ggttgcctat taagtgttga aaatagggtg
      421 gcactttaat tttatctgaa ttttttgccg aaaagtcttc gttcaaaaca tcaatttgcg
      481 acttaatcat atcatcgctg atattttgag ccgctgtatt ataaactacg tgtactacta
      541 ccggtatggt aactacggct tgtgtgccac tgctgttttc atttgccaac tgtgtttgca
      601 aaacttgctc ttgctgctcc atgcgttgca acaatgcagg gtcgtctttt attaactgat
      661 ctaaatattg catggtgccg cagcggtcgg tttgctgggc attagccaaa ttgaaaaaac
      721 ttgaaaaggc tattgccgaa agtaatttta atttcatttt ataattattt gggggttact
      781 taattaattg gtcaaatata gtttttgagg acaaaggctg atataaaaaa atgttgtttg
      841 aatcattttt acattctgaa cataccaaac ttaaaaaaat ttgttttagg ccagggctaa
      901 attattctgc ttatccacct ttaacagttc atggtcgagc atccagttta acacagccaa
      961 tgctttttcg gggtgccttg tattcaggct ttttaatagc gactcatcgt caaaagaacc
     1021 gtgtgcaatg tgctccttaa tgctttgcat gattgcttca aactcaattt cattaatgtt
     1081 taacttgtta cggttgcggc aataatcaca aacgccacat cgctgtttta tttcttcatt
     1141 aaaataactc aacagcatta aactacggca tttgtgtttt tcttcggcat attgtgtcat
     1201 agcttttagt ctgatttcgt agcgtttttt acgcgctgcc aaaacgctta ccgaaatttc
     1261 taaatgtgta ttaggcaccc gttgatggag gaaagtaaga agcggcttat cggtttgctt
     1321 caaatattct ataacaccat atttttgaag tagtaatagc ttattaatta gttccggtat
     1381 ttcgagtttg cagcgttgtg ctatttcgcc ttcatgaatt tgtacaaaat cgtcaaacaa
     1441 accaccatag gaacgaagca atgttttaat taatacatct aattcggggt gctccatttg
     1501 aaatctgtac aaatcctcat tttgcaacaa tatatgtaaa cgcgatggca taaaaacacc
     1561 atcggttaaa ccgattaatt tatccatttc caaaatgtgc aatacggctg aaacctggta
     1621 ggcatccaat ttaaatacct tacaaaaatt tactatatca aattcaaatt catcatttaa
     1681 tccaaagcct gttgctatct ggaggtaatt acataaagca ctatacacat ttctgataac
     1741 atctaatacc ggaaaatttt gcgctacacg ctgatgcata tctgcaatat cgtttgagtt
     1801 gaacagaagt actgcaaaag ctagatgctc atcacgacct gccctgcctg cttcctgata
     1861 atatgcttct aaattatcag gcatatcaat atgaattacc aaacgcacat cgggtttatc
     1921 aatgcccata ccgaatgcat tggttgcaac cataacgcgg attttatttt ttatccagtc
     1981 ttcttgcttg ttgttacgct gcatggtagt taatccggca tgataaaaat cggccgtaat
     2041 gttatgttga tttaaataca aagcggtttc ctgtgtacgt ctgcggttac ggcaataaat
     2101 aatagccgag cctgttacac tatttaaaat ctgcaaagca cgtagttgtt tatcatcaac
     2161 ttcgtttact acataggtta ggtttttgcg ttcgaaactt tttataaata cattttg
//
LOCUS       DAOMAA010000316         2215 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_46559_length_2215_cov_3.450463, whole genome shotgun sequence.
ACCESSION   DAOMAA010000316 DAOMAA010000000
VERSION     DAOMAA010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46559_length_2215_cov_3.450463"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="PLO59_05050"
     CDS             complement(<1..692)
                     /locus_tag="PLO59_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00506.1"
                     /translation="MVKTDSMGLFQWNKRFGGHDGDAGFRVLQSADGGFYFLGISQSG
                     MGQQVTDTNHGGFYEAWLVRTDVNGNKLWDVMLGSYDDEILKDAVTLADNSIVLAGST
                     SAYVPNGTVSQPNLGPFTTTDVWIVKVDATGQVLWDRRYGGMGDDLGEGICLASDSGF
                     YVTGSVHRSSHLPDVTDTTDKGIIAVWLLKLNSMGYKEWDKKFGGDSISEGLEVLFSV
                     STKSIVVCGVSIS"
     gene            complement(693..1109)
                     /locus_tag="PLO59_05055"
     CDS             complement(693..1109)
                     /locus_tag="PLO59_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00507.1"
                     /translation="MKQLIILMLCLPFALSGQGIVTDWQKSVGDYAGDSPGIWGSHFI
                     CHGPNKSWLLGCASRSQIGGDKSQAPCNDQGIDSDFWQIKFDSTGQKVWDYKYGSLDY
                     DEGNCIIATHDGGYLLAGRSFGEDCDKTQSAHLVDY"
     gene            complement(1111..1380)
                     /locus_tag="PLO59_05060"
     CDS             complement(1111..1380)
                     /locus_tag="PLO59_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00508.1"
                     /translation="MRNGTPAQVVTQALFYEDLLHTGLQLPNEDNMHLTLMHYNGQQL
                     LNVKGNSDKLNQILLSQYDKAAPGLLILTLNTAKQTFIFKLLKTQ"
     gene            complement(1402..>2215)
                     /locus_tag="PLO59_05065"
     CDS             complement(1402..>2215)
                     /locus_tag="PLO59_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00509.1"
                     /translation="PSVTTIYYTYTAAGDRKVVVQDYKIVGNINNSFTAICDGNNGSL
                     PAANVDVIDELTAPPPPLNNGFAGVRIACHREDNDVNILQEGFIIVYQRIGIVAVSPT
                     LQQTHEVLGRAFFCSSNSACKYFPPLNNPANNYCNGSLQFQGVNPTQTLPDINSVMEE
                     NKLPQITLDRFTGTFDSNPNADGNTAILTWNYYDGTTYKHRPVMLYINIQTGLPQVTP
                     NFTPTSQANLYLFNNVSTTNDNQIAVSVCSETGNTRHILYAWVDDANNIVKY"
BASE COUNT          642 a          473 c          416 g          684 t
ORIGIN      
        1 gatatgctaa ctccacaaac aacaatactt tttgtagata cagaaaataa aacttccaat
       61 ccttcactta tactatcacc gccaaacttt ttatcccatt ctttatagcc catactattt
      121 aatttaagca accaaactgc aataatgcct ttgtctgttg tgtcggtaac atcgggtaaa
      181 tggctactgc gatgcactga accggttaca taaaaaccac tatcggacgc caaacaaatt
      241 ccttcaccta aatcatcgcc catgcctcca tagcgcctgt cccatagcac ttgtcctgta
      301 gcgtccactt ttacaatcca aacatcggtg gtagtaaaag ggcctaagtt gggttggcta
      361 accgtaccat tgggtacata ggcactggtg ctgcctgcca atacaatact gttatcggcc
      421 aaggtaacgg catcttttaa aatttcatca tcgtagctgc ccagcattac atcccatagt
      481 ttattgccat taacatcggt acgaaccaac caggcttcgt aaaagccacc atggttggta
      541 tcggttactt gctggcccat accactttgc gaaataccta aaaagtaaaa accaccatcg
      601 gcactttgca ataccctaaa tccggcatca ccatcgtggc cgccaaatcg cttattccat
      661 tgaaacaaac ccatgctatc ggttttaacc attcagtaat ctaccagatg tgcactttgt
      721 gttttatcgc agtcttcgcc aaaggagcgg ccggctaata gatagccgcc atcatgggtt
      781 gcaataatgc aattaccctc gtcataatcc aagctgccat atttatagtc ccaaactttt
      841 tggcctgtag agtcgaattt tatttgccaa aaatcgctgt caatgccttg gtcattacaa
      901 ggagcttggc ttttatcgcc acctatttgg cttctactgg cacatcccaa aagccatgac
      961 ttattaggcc catgacaaat aaaatggctg ccccaaatac cgggagagtc accggcatag
     1021 tctcctacac ttttttgcca atcggtaaca atgccttgac cgcttaatgc aaaaggcagg
     1081 cataacatta atattattaa ttgtttcatt ttattgggtt ttaagtagtt tgaaaataaa
     1141 tgtttgtttt gctgtgttta gcgttaaaat taataaaccc ggtgctgctt tatcatactg
     1201 gcttagtaaa atttgattta acttatcgct gttacctttt acgtttaata attgctggcc
     1261 gttgtaatgc attaatgtta agtgcatatt atcttcattg ggtaattgca atcctgtgtg
     1321 taataagtct tcataaaaca gtgcttgtgt tactacctgt gcaggtgtac cattacgcat
     1381 aataaaaccg gtatttacgt gttaatattt tacaatatta ttggcatcat ccacccaggc
     1441 atataaaata tgacgggtat tacccgtttc ggaacatacc gatacggcaa tttgattatc
     1501 gttagttgta gaaacattat taaacaagta caaattagcc tgagatgtag gcgtaaaatt
     1561 aggcgtcacc tgtggtaaac ctgtttggat attgatatag agcataacgg gcctgtgttt
     1621 ataagtggtt ccatcatagt aattccaggt taatatggct gtattgccat cggcattggg
     1681 gtttgagtcg aatgtaccgg taaatcggtc taaagtaatt tgtggtagtt tgttttcctc
     1741 catcacactg ttaatgtctg gtaaggtttg cgttgggtta acgccttgga attgtaaact
     1801 tccgttgcaa taattattag caggattatt taatggagga aagtatttgc aagctgaatt
     1861 tgaagagcaa aaaaaagcac gacctaaaac ttcatgcgtt tgttgcaggg ttgggctaac
     1921 ggcaacaata ccaattcgtt gatatactat aataaagcct tcttgcaata tgttgacatc
     1981 gttatcttcg cggtggcagg caatacgtac ccctgcaaag ccattgttta atggaggagg
     2041 tggggcagta agctcatcaa ttacatccac attggctgct ggcaaactgc cattattgcc
     2101 atcgcaaatt gcagtaaaac tattattaat attacccaca attttataat cctgtaccac
     2161 tacttttctg tcgccagcag cagtataagt ataataaatt gtggtaaccg atggt
//
LOCUS       DAOMAA010000317         2214 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_46630_length_2214_cov_2.490505, whole genome shotgun sequence.
ACCESSION   DAOMAA010000317 DAOMAA010000000
VERSION     DAOMAA010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46630_length_2214_cov_2.490505"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1645
                     /locus_tag="PLO59_05070"
     CDS             <1..1645
                     /locus_tag="PLO59_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HQV00510.1"
                     /translation="LEEIISEDISNDKHAGRILTRFPPEPNGYLHIGHAKSICLNFGL
                     AQTFNGKTNLRFDDTNPITEKTEYVESIKADIRWLGFVWEAEYYASDYFEQLHDFAVK
                     LITKGLAYVDDLSAEEIARLKGTPTEAGLPSPFRNRPIAENLELFAAMRNGIYADGEK
                     TLRAKIDMASPNMHLRDPIMYRIKHAHHHRTGNKWCIYPMYDFAHGQSDAIEKITHSI
                     CTLEFEVHRPLYNWFIEQLEIFPSRQIEFARGNINYTITSKRKLLQLVTEGHVAGWDD
                     PRMPTISGMRRRGYTPASIRDFCDRIGIARRENMIDVGLLEFCVREDLNKNAQRRMVV
                     VNPIKLIIDNYPDDTTEMLTAENNTEDPNAGSRLMPFSKNLWIEADDFTENPPKKYFR
                     LAPGLSVRLKSAYIITCTGCDKDENGNVIAVHANYHPNSKSGQDTSGISVKGVMHFVS
                     AAQALPCTINLYERLFQVENPASEAADFLSYLNPDSLKVITNAFAEPVLADAQPLQTF
                     QFLRLGYFCADTLSKPNQLIFNRTVTLKDTWAKEVKKSN"
     gene            1648..1989
                     /gene="folB"
                     /locus_tag="PLO59_05075"
     CDS             1648..1989
                     /gene="folB"
                     /locus_tag="PLO59_05075"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HQV00511.1"
                     /translation="MQLHITDIDCYAYHGCLKEEAAIGGHFSVSLTLTYDFTEAAQTD
                     NLTQAIDYVLVHAEVRKQMAIRANLIEHVATRIKQSMQKLFPQCQNITVAVTKYNPPV
                     NGHINKVTVTI"
BASE COUNT          739 a          480 c          408 g          587 t
ORIGIN      
        1 tttagaagag ataataagtg aagatataag caatgataaa catgcaggcc gcatacttac
       61 ccggtttcca cccgagccca atggctacct gcacattggc cacgctaaaa gtatttgtct
      121 caattttggc ttagcccaaa catttaatgg caaaaccaat ttacgttttg atgataccaa
      181 tcccataacc gaaaaaaccg aatatgttga aagtattaaa gccgatatac gttggctggg
      241 ttttgtatgg gaagcagagt actatgcatc cgattacttt gaacaactgc atgattttgc
      301 cgtaaagctt attacaaaag gattggctta tgtagatgat ttatcagccg aagaaattgc
      361 acgcctgaaa ggcacaccaa ctgaagccgg attaccatcg ccatttcgca accgccccat
      421 tgccgaaaac ttagaactgt ttgcggctat gcgaaacgga atttatgctg atggcgaaaa
      481 aacattacgg gcaaaaattg atatggcatc gcccaatatg cacctgcgcg acccaattat
      541 gtatcgtatt aaacatgcac accatcaccg tacgggtaat aaatggtgca tatatcccat
      601 gtatgatttt gcacatggac aaagcgatgc catcgaaaaa attacacact caatttgcac
      661 cttagaattt gaagtacacc gcccacttta caactggttt attgagcagc tcgaaatttt
      721 tccaagccgg caaattgaat ttgcacgcgg taacataaac tacaccataa ccagcaaaag
      781 aaaactatta caactggtta ccgaaggaca tgtagccggc tgggacgacc cacgtatgcc
      841 taccattagt ggtatgcgca ggcgtggtta cacgcctgca agcatacgcg atttttgcga
      901 ccgcattggc atagcccgca gagagaatat gattgatgta ggcctgctcg aattttgtgt
      961 acgcgaagac ctgaataaaa atgcacaacg cagaatggta gttgttaacc caattaaact
     1021 catcattgac aattatccgg atgatacaac tgaaatgctt acggccgaaa acaacaccga
     1081 agacccaaat gccggaagcc gtttaatgcc ttttagcaaa aacctttgga tagaagccga
     1141 tgattttacc gaaaacccac caaagaaata tttcagattg gcacctggct tatcagttcg
     1201 cttaaaaagt gcttacatta taacttgcac aggctgcgat aaagacgaga atggaaacgt
     1261 tattgctgta catgcaaact atcatcccaa cagtaaaagc ggacaagata ccagcggtat
     1321 atccgtaaaa ggcgttatgc attttgtaag tgcggcacaa gctttacctt gcaccataaa
     1381 tttgtacgaa cgcttatttc aggtcgaaaa ccctgcaagc gaagcagccg actttttatc
     1441 gtacctaaac cccgatagct taaaagttat tacaaacgct tttgccgagc cggtcttagc
     1501 cgatgcacaa ccattgcaaa cttttcagtt tttaagactg ggctattttt gtgccgatac
     1561 cctttcaaaa ccaaaccaac ttatatttaa ccgcaccgtt acacttaaag atacctgggc
     1621 caaagaggta aaaaaatcaa attaaaaatg caactacaca ttaccgatat tgattgctat
     1681 gcataccacg gatgcttaaa agaagaggca gctattggtg gccattttag tgtgagctta
     1741 actttaacct atgattttac ggaggcagct caaacggata acctcacaca agccattgac
     1801 tatgtattgg tacatgccga agtaagaaag caaatggcca ttagagccaa tttaattgaa
     1861 cacgttgcaa cacgtatcaa acaaagcatg caaaagcttt ttccgcaatg ccaaaacatt
     1921 acggtagcag ttacaaaata taatccaccg gtaaacggcc atatcaacaa agttacagtt
     1981 actatatagg ctttttaatt cttgattttt tattcgctcc taaaaaatct tttttgaata
     2041 agattcatca acctataaaa agtaggcatt ggtcaataat ttgccagata aaatgttaaa
     2101 atacttgtaa tttaatttcg cccattatac aattgtaaac ccctaaaatt ttatacggct
     2161 tctgttaact atttaaacga agtctgcccc ctaaaattta attgcaattt atag
//
LOCUS       DAOMAA010000318         2213 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_46641_length_2213_cov_5.458295, whole genome shotgun sequence.
ACCESSION   DAOMAA010000318 DAOMAA010000000
VERSION     DAOMAA010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46641_length_2213_cov_5.458295"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2213)
                     /locus_tag="PLO59_05080"
     CDS             complement(<1..>2213)
                     /locus_tag="PLO59_05080"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - viral entry into host cell
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HQV00512.1"
                     /translation="RNIFTGAGAGAALTLGADNAFFGWNAGATNAVAGCNTFIGSEAG
                     ANITTGGNNAFAGYRSGYQNTTGINNAFFGPVSGRANTTGLHNVFIGGGAGSNNTTGN
                     FNVYLGNLAGQGNTIGSNNVIVGDRAGSSYIGSNNVLIGFNAGILTTAAGQTFIGYRT
                     GWSTTTGASNCFTGFECGFANTTGSFNVFMGYHSGSDNTTGTNNTYMGFETGAANTTS
                     AGNTFTGYRAGWLNTAANNTFMGMEAGAGNVNATGLTFVGYRAGTGANTGSGNTFLGY
                     ETGFANTAGDENTFCGYNSGKTNATGKRNTFVGSLAGLANTGNDNSTFLGYQAGTAST
                     GRENTFVGSNSGLRNTSGYNNVFLGHASGVQITTGNANTYLGWNTGPATSSYAAISNS
                     AAIGANAEVLNSNHIILGDNQVSVGIGLSGDATGPTAKLDVREPRLLASPVVTGINTI
                     VSGANTENRAGTFLAQGATNNYGIKATATGTATYNAGADFLAASATTFNIGVSAYGHF
                     GTTGIGGRFVSTGCTGSSTGVSGLANSPFATNNYGGMFIAGGGATNNYGLHVTASNAT
                     SINYGIYASNLTTGSTCTGVGCANAAGYFQGPICVTNLSYIPSDLSLKTNVTPIINAR
                     AVLTQLQPKSYYYDTLQYDMNLAQGLQYGFIAQELEMVLPQLTRQFVQPPTQDSSGNI
                     ITPQVNFTAIAYQPLIALLIAGYKEQQQQIDSLANLINNNANPKQPLQSMSVTLS"
BASE COUNT          571 a          514 c          499 g          629 t
ORIGIN      
        1 tgcttaacgt aacagacatg gattgcaatg gctgttttgg attggcgttg ttgtttatta
       61 gattagctaa ggagtcaatt tgttgttgct gctctttata tccggctatt aaaagtgcaa
      121 taagtggttg gtaagcaatt gccgtaaagt ttacctgtgg agttataata tttcctgagg
      181 aatcttgcgt tggcggttgc acaaactgac gcgtaagttg cggcagcacc atttccaact
      241 cctgagcaat aaagccatat tgtaagcctt gtgctaaatt catatcgtat tgcagtgtat
      301 cgtaataata agatttaggt tgtagttgtg ttagtactgc tcttgcattt attatagggg
      361 taacatttgt ttttaagctt aaatcggaag gtatgtagga taggttggta acacaaatgg
      421 ggccttgaaa atatccggct gcattggcac agcctacacc tgtgcaggtg ctgcctgtag
      481 ttaaattaga tgcgtatatg ccataattaa tacttgtagc attgcttgca gtaacatgta
      541 agccataatt gtttgtagcg ccacctcctg caataaacat tccgccataa ttattggttg
      601 caaaagggct gttggccaaa cccgatactc cggtacttga accggtacac cctgtggata
      661 caaagcggcc gccaatgcct gttgtaccaa aatggccata agcgctaacg cctatgttaa
      721 acgtagtagc ggatgcagcc aaaaaatcgg caccggcatt atatgttgca gtacccgtag
      781 cggtagcttt tataccatag ttgttggttg caccttgggc taaaaaagtg ccggctctgt
      841 tttcggtatt agcaccgctt actatcgtat ttataccggt aacaacgggc gaagctaata
      901 agcgcggttc gcgcacatct aatttagccg taggcccggt tgcatcgccc gaaaggccga
      961 tgcctaccga tacctggtta tcgcccaaaa taatatggtt gctgttaagc acttcggcat
     1021 tggcgcctat tgcggccgag ttgctaatgg ctgcatagct gctggtggca gggccggtat
     1081 tccaacctaa gtaggtgttg gcatttccgg tagttatctg cacgcctgaa gcatgcccta
     1141 aaaaaacatt attgtaacct gatgtattgc gcagcccgct gttgctaccc acaaatgtgt
     1201 tttcgcgccc ggtagatgcc gtgcctgcct gataacctaa aaaggtgctg ttgtcgttgc
     1261 cggtattagc caacccggca agcgagccta caaaggtatt gcgtttgccg gtagcattag
     1321 tcttgccgct gttgtaacca caaaaagtat tttcatcgcc cgctgtattt gcaaatccgg
     1381 tttcatatcc taaaaaagta ttaccactgc ccgtatttgc gcctgtgcct gcacgatatc
     1441 ctacaaaagt aagtccggtt gcatttacat tacctgcacc ggcttccata cccataaagg
     1501 tattgttagc tgccgtgttt agccagcctg cacgataccc tgtaaatgtg ttgcctgccg
     1561 aggttgtatt tgcagctcct gtttcaaatc ccatataagt gttgtttgta ccggtggtat
     1621 tatcgctgcc cgaatgatag cccataaaaa cattaaacga gccggtagta tttgcaaaac
     1681 cgcactcaaa acccgtaaag caatttgatg cacctgtagt agtactccaa ccggtacggt
     1741 acccaataaa tgtttgaccg gctgctgtgg ttaatatacc tgcattaaat ccgataagta
     1801 cattgttact gccaatgtag gatgaacctg cacggtcgcc cacaattaca ttgttacttc
     1861 caatagtatt accctgccct gccaaattgc ctaaatacac attaaaatta cctgtggtgt
     1921 tattactgcc ggcaccgcca ccaataaaaa cattgtgcaa ccctgtagta tttgccctgc
     1981 ccgaaaccgg cccaaaaaag gcgttgttaa tgcctgttgt attttggtat ccactacggt
     2041 atcctgcaaa ggcattgttg ccacccgtag ttatattggc cccggcctct gagccaataa
     2101 aagtattgca acccgcaact gcattggtgg caccggcatt ccaaccaaaa aaggcattat
     2161 cggcacctaa ggtaagtgct gcaccggcac ctgcaccggt aaatatgttg cgg
//
LOCUS       DAOMAA010000319         2212 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_46689_length_2212_cov_2.941586, whole genome shotgun sequence.
ACCESSION   DAOMAA010000319 DAOMAA010000000
VERSION     DAOMAA010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46689_length_2212_cov_2.941586"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="PLO59_05085"
     CDS             complement(<1..617)
                     /locus_tag="PLO59_05085"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003000201.1"
                     /note="part of NADH-ubiquinone oxidoreductase complex I;
                     shuttles electrons from NADH, via FMN and iron-sulfur
                     (Fe-S) centers, to quinones in the respiratory chain; NuoF
                     is part of the soluble NADH dehydrogenase fragment, which
                     represents the electron input part of NADH dehydrogenase;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit F"
                     /protein_id="HQV00513.1"
                     /translation="MPTKLLLNNEHIPGINTLQVYRNNGGYASVEKALKQMTPEAIVE
                     EVKISGLRGRGGAGFPTGMKWSFLAKPAGVPRYLVCNADESEPGTFKDRYLMEKIPHL
                     LVEGMITSSYALGCNTAYIYIRGEYFYVSRILENAIAEAYAAGYLGSNILGTGYNLNL
                     YVQVGGGAYICGEETALLESLEGKRGNPRIKPPFPAIAGLYGCPTV"
     gene            complement(670..1194)
                     /locus_tag="PLO59_05090"
     CDS             complement(670..1194)
                     /locus_tag="PLO59_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HQV00514.1"
                     /translation="MNNEIKFSAEALAIVKQMISRYPDGKQKSALLPVLHLAQAEFDG
                     WLSVPVMDYVASLLSIKPIEVYEVASFYSMYNLKPVGKCTLEVCRTSSCWLMGAEDIV
                     RHIQNRLGIKDGQTSSDGMFTLKTVECLGSCGTAPMLQCGAKYHENLTLEKVDELLDK
                     LATDNKRTTYLDIV"
     gene            complement(1208..>2212)
                     /locus_tag="PLO59_05095"
     CDS             complement(1208..>2212)
                     /locus_tag="PLO59_05095"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009087729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HQV00515.1"
                     /translation="QITPITDRLNYCSSPINNIGWHMTVEKLAGISIPKRVEYMRVII
                     MELARIADHIVCNTVIGVDTGAMTGFLYVFQWREKIYEIYEEICGARLTTNIGRIGGF
                     ERDFPQTAWQKLDDFIRDFPAALQEFEKLLVRNRIFMDRTINCGPISAERALAYSFTG
                     PNLRAAGVDYDVRAMNPYCSYQDFDFIIPIGKHGDTYDRFMVRNQEMWESLNIIKQAL
                     QKMPAGNEYHADVPEFYLPEKADVYSKMEALIYHFKIVMGETQIPVGEVYHAVEGGNG
                     ELGYYLISDGGRTPYRLHFRRPCFIYYQAYPEMIKGTYLSDAILTMSSMNVIAGELDA
                     "
BASE COUNT          637 a          495 c          386 g          694 t
ORIGIN      
        1 acggtggggc atccatataa accagcaatg gccggaaatg gtggctttat acgtggattg
       61 ccgcgtttac cctctaagga ctcgagcaaa gccgtttctt cgccacaaat ataagcacca
      121 ccgccaactt gaacatataa attaagattg tatccggtgc ctaaaatatt actacctaaa
      181 tatcctgctg cataggcttc ggctatggcg ttttctaaga tacgcgaaac gtaaaagtac
      241 tcaccgcgta tataaatgta ggctgtatta caacctaatg catagcttga tgtaatcata
      301 ccctcaacca atagatgagg tattttttcc atcaagtagc ggtctttaaa tgtgcctggc
      361 tcactttcgt ctgcattgca aaccaagtag cgtggcacac ctgcaggttt agctaaaaaa
      421 ctccatttca ttccggtcgg aaaaccggct ccacctctac ctcttaaacc gcttatttta
      481 acttcttcta caattgcttc gggcgtcatt tgtttcaacg ctttttctac cgaagcatag
      541 ccaccattat ttcgataaac ctgcaatgta tttatgccgg ggatatgctc gttattaagt
      601 aaaagttttg ttggcatttt ataaatagtt ctttaagctg aaattaatgt ctcccactga
      661 ttattatttt taaacgatat ccagataggt ggttctttta ttgtctgttg ccagtttatc
      721 taacaattca tctacttttt ccaaggtaag gttttcgtga tatttggcac cacattgtaa
      781 cataggcgct gtaccacacg aacccaaaca ctcaacggtt tttaatgtga acatgccatc
      841 ggatgaagtt tgtccatcct ttatgcccaa tctattttga atatgtcgca caatatcttc
      901 ggcacccatg agccagcatg aactggtgcg gcatacctct aaagtacatt tgcctacagg
      961 ttttaaatta tacatgctat aaaatgatgc aacttcataa acctcaatag gcttaatgct
     1021 taatagcgaa gctacgtaat ccataacagg cacgcttaac caaccatcaa attcagcttg
     1081 tgccaaatgt aaaactggta gcaacgctga tttttgtttt ccatcaggat acctgctaat
     1141 catctgtttt acgattgcta aagcttcggc cgaaaatttt atctcattat tcataatatt
     1201 caaattatta agcatctaac tcgcctgcaa ttacattcat cgaactcatg gttaaaatag
     1261 catcacttaa ataggttcct ttaatcattt cggggtaagc ttgataataa ataaaacatg
     1321 gtcttctgaa atgtaaccta taaggtgtac gaccaccgtc actaattaag taatagccta
     1381 actcgccatt gccaccttca acagcatgat atacctctcc taccggaatc tgggtttcgc
     1441 ccatcacaat tttaaagtga taaattaatg cctccatttt actataaaca tcagcctttt
     1501 cgggtaaata aaactcgggc acatcggcat gatattcatt gcctgcaggc attttttgta
     1561 atgcttgttt tatgatgttt aaactttccc acatttcctg attgcgtacc ataaacctgt
     1621 cgtatgtatc gccatgtttg ccaataggta ttataaaatc aaaatcctga tagctacaat
     1681 atggattcat tgcacgcaca tcgtaatcaa cacctgcagc ccgcaagttt ggacctgtga
     1741 agctataagc taaggcgcgt tctgccgaaa ttgggccgca gtttatggtg cgatccataa
     1801 aaattctgtt gcgcaccaat agtttttcaa attcttgcaa cgctgccgga aagtcgcgta
     1861 taaaatcatc taatttttgc catgctgttt gcggaaagtc gcgttcaaaa ccacctattc
     1921 gaccaatatt ggttgttagg cgcgcaccac atatctcctc atatatttca taaatttttt
     1981 cgcgccattg gaatacgtat aaaaaacccg tcatagcacc tgtatccacg cctataacgg
     2041 tattgcaaac aatatgatcg gcaatacgcg cgagttccat aattattacc cgcatgtatt
     2101 caacccgttt gggaattgat atacctgcta atttttcgac tgtcatgtgc cagccaatat
     2161 tgtttatggg cgatgagcag tagttaagcc tatcggtaat gggtgttatt tg
//
LOCUS       DAOMAA010000320         2210 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_46775_length_2210_cov_2.613457, whole genome shotgun sequence.
ACCESSION   DAOMAA010000320 DAOMAA010000000
VERSION     DAOMAA010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46775_length_2210_cov_2.613457"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..1074
                     /locus_tag="PLO59_05100"
     CDS             103..1074
                     /locus_tag="PLO59_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015363213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQV00516.1"
                     /translation="MKIGVLGAGHLGKIHIKLLKDITQWQLVGFYDADIEKAKAYSQI
                     IGVPYFDSDTDLINQCDAIDIVTPTKYHHACAVKCLKAGKHIFVEKPVAASIREAKEM
                     IKLASEANVKVQIGHVERFNPAFLAAQHQIQSPMFIETHRLAQFNPRGTDVSVILDLM
                     IHDIDIVLSVVKSPVRKISASGVAVVSDTPDIANARIEFDNGCVCNLTASRISLKNMR
                     KSRFFQRDAYVSVDFLKKTTELVRMKTVVGEQDPLGIYLELGEGKGVKQIYFENPNIT
                     DSNAIQMELTLFAQAILDDTEPKVSLNDGYEVLHVAHQIIDKLKNIG"
     gene            complement(1135..>2210)
                     /locus_tag="PLO59_05105"
     CDS             complement(1135..>2210)
                     /locus_tag="PLO59_05105"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV00517.1"
                     /translation="TIPAAQFASDVIIDDNYIYAATSVGIYKYNKNSKQFVTNNTATG
                     IRKMCVWNNQLLVSRGDIGLNNYFFQAYDKNTLALIYELDVTNGPAYSTEGISVVGDS
                     CYIALNNGFNFPNYTGYIGVIYLPTATYVRQFDLGPIGLNPDYFAIKNGVIHTLNNRD
                     FTNASISTYELATGQLATTDLQISSGCGTSVLAGTEILYQVMGATTLQKFSLSSQSTY
                     DSLLINKPVYGLAHEDVANLIYAGETDFVSTGQVFIYNMAGLALDSFMVGVSPGNMVI
                     DYNSTSGIAENVSQLNVYPNPATNQIYLGNQKPASYQILNVTGIKVAEGVALPQANIN
                     ISNLSNGIYFLKTQSGTYKFNKQ"
BASE COUNT          727 a          382 c          409 g          692 t
ORIGIN      
        1 actaaaatac aaatttcagc gcgttaatac acagcacttt actttcggtg ctgccataaa
       61 atggctcctt tttaaaagaa aaaaactcaa ctttaaaaaa ctatgaaaat tggtgtatta
      121 ggtgcaggcc atttgggcaa aattcatata aaacttttaa aagatataac acaatggcaa
      181 ctggtaggtt tttatgatgc tgatatagaa aaagcaaagg catatagtca aattataggt
      241 gtaccctatt tcgatagtga taccgattta ataaatcaat gcgatgcgat agatattgtt
      301 acgccaacca aataccatca tgcttgtgcg gtaaaatgtt taaaagcagg taaacatata
      361 tttgtcgaaa agcctgtggc agcttctata cgcgaagcta aagagatgat aaaacttgcc
      421 agcgaagcaa atgtaaaagt acaaataggg catgttgagc gatttaaccc ggctttttta
      481 gccgcacagc atcaaataca atcgcccatg tttattgaaa cacaccgttt ggcgcagttt
      541 aatccgcgag gtaccgatgt ttcggtaatt ttagatttga tgatacatga tattgacatt
      601 gttttaagtg tagtaaaatc gcctgtacga aaaatttcgg caagcggtgt agccgttgtt
      661 agcgatacgc ccgatattgc aaatgcacgt atcgaatttg ataatggttg tgtttgtaat
      721 ttaactgcaa gccgcatatc gcttaaaaac atgcgtaaaa gccgtttctt tcaacgcgat
      781 gcttatgttt cagttgattt tttaaagaag actacggaat tagttcgtat gaaaacagtt
      841 gttggcgagc aagatccttt aggcatttat ttagaacttg gcgaaggcaa gggtgtaaaa
      901 cagatttatt ttgaaaaccc aaacattacc gattcgaatg ccatacaaat ggagttaact
      961 ttatttgcac aagctatttt agatgacact gaacccaaag tgagtttaaa tgatggttat
     1021 gaggttttac atgtggcgca tcagattatt gataaactta aaaacattgg ctaaaagtga
     1081 aagcaaaaaa aaagtggacg ttctgtccac ttttttttgt atattttaaa tttactactg
     1141 cttgttaaat ttataggttc ccgattgggt tttcaaaaaa taaatgccgt tacttaaatt
     1201 gctaatattg atattagcct gaggcaatgc aacaccttcg gcaactttaa ttccggttac
     1261 atttaatatt tggtatgatg caggtttttg gttacctaaa taaatttgat tggtagctgg
     1321 gtttggatat acatttagtt gactaacatt ttcagcaata cccgaagtac tgttatagtc
     1381 aataaccata ttgccgggcg atacaccaac cataaaacta tctaaagcca aaccggccat
     1441 gttataaata aatacctggc ctgtagaaac aaaatccgtt tcgcctgcat aaatcaaatt
     1501 cgcaacatct tcatgcgcta aaccatacac cggtttattt ataagtaatg agtcgtaggt
     1561 tgattgtgaa ctcaagctaa atttttgtaa tgtagttgca cccataacct gatataaaat
     1621 ttcggtaccg gcaagtaccg atgtgccaca gccactacta atttgtaaat cggtggtggc
     1681 taattggcca gtagctaact cataggttga gatagatgca ttggtaaaat cgcgattatt
     1741 tagggtatgg ataacaccat ttttaattgc aaaataatcg gggttaagtc ctatcggacc
     1801 taaatcaaac tgacgtacgt aagttgcagt aggtaagtat atcacaccaa tgtaccctgt
     1861 ataattcgga aaattaaatc cattatttaa tgcgatataa caactatcac ccaccacaga
     1921 aatgccttcg gttgaatatg ccggtccgtt tgtaacatcc agttcataaa tgagtgctaa
     1981 tgtgttttta tcataagcct gaaaaaaata attattcaat ccgatatcgc cacgcgatac
     2041 taataactgg ttattccata cacacatttt cctgatacct gttgctgtat tgtttgtaac
     2101 aaactgtttg ctgtttttat tgtatttata tataccaaca ctggttgcag cataaatata
     2161 attgtcatca ataattacat cgcttgcaaa ttgtgcagca ggtatagtgt
//
LOCUS       DAOMAA010000321         2203 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_47076_length_2203_cov_2.809125, whole genome shotgun sequence.
ACCESSION   DAOMAA010000321 DAOMAA010000000
VERSION     DAOMAA010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47076_length_2203_cov_2.809125"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..679
                     /locus_tag="PLO59_05110"
     CDS             23..679
                     /locus_tag="PLO59_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HQV00518.1"
                     /translation="MLDKNGIAKRIAREVQDGYYVNLGIGIPTLVANYIPEGMHVVLQ
                     SENGILGMGPFPVEGTEDADLINAGKQTVTLLPGSSIFDSAMSFGMIRSQKVNLTILG
                     AMEVSEFGDIANWKIPGKMVKGMGGAMDLVASAKNIIVAMQHVNKAGESKLLPKCELP
                     ITGLRCVKKIVTELAVLDVLPQGGFKLLERAPGVSVEQIVKATAGKLIVEGDIPEMVL
                     "
     gene            693..2195
                     /locus_tag="PLO59_05115"
     CDS             693..2195
                     /locus_tag="PLO59_05115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HQV00519.1"
                     /translation="MQQLLFVLKKPQTVAAIFFVLLIAMLGNIGLQKAIPLRPQSIHQ
                     WAQCDRASVALNYALKRTTFFKPMVHECSNGSGISGMELPVVNYGASVLYQLFGYHEA
                     YYRIWVMLIAIAGIVAAYYLACLFLNQWWALAAVLLFIQSPVLLYYMPNFLPDAASLG
                     LSLIAWWLFFKIIGQKSNTLLLWFFLIVSLAGLIKVTALINTITMLLFVVIQNHKRGW
                     RSLLTNNVLCALVVSIIITGAWYIYARWLSVQYHSYYFLMAPHYPTNLADAYKTWLRI
                     KTGMLNEYYTAVLGYILLAGTIALAFNFKKVQRQLLFVTVTLYAGSFLFFMLMLNQFV
                     YHDYYFIALMPAVLFHLMLIANLVQKLNFKMQLGIALIIVVAANYGMNICKKNFASRY
                     NEQSWYYNNAKSKAYFNFETVLRENGIASDEKILSFTDETPNTSLYLMNQRGISLGAD
                     TNLWHPYLKAGYKYAVLNDSLLLHSGLQAFLDKRLTYKFGLYLYTLKPQQ"
BASE COUNT          669 a          378 c          441 g          715 t
ORIGIN      
        1 caaataaaat ttcaataaaa tcgtgttaga caaaaacgga atcgcaaaac gcatagcacg
       61 cgaagtacaa gatggttact atgttaacct gggcattggc atacctacac tggttgccaa
      121 ttatatacct gaaggcatgc atgtggtttt acaatcagaa aacggcatac tgggtatggg
      181 cccttttcct gttgaaggta cagaagatgc cgatttaata aatgcaggta aacagacggt
      241 tacattattg cccggttcat ccatattcga ctcggcaatg agttttggta tgatacgttc
      301 gcaaaaagta aacctaacca tattgggtgc catggaagta agcgagtttg gcgatattgc
      361 caattggaaa attcccggta aaatggtaaa aggtatgggt ggcgcaatgg atttggttgc
      421 atcggcaaaa aatataattg tagcaatgca acacgtaaat aaagctggcg aatcaaaact
      481 tttacctaag tgtgaattgc ctattacagg cttgcgttgc gtaaaaaaaa ttgtaaccga
      541 actggctgta ctggatgtat taccgcaagg tggttttaaa ttactggaac gagctcccgg
      601 tgtaagtgtc gaacaaatag ttaaagcaac tgccggaaaa ctgatagtag aaggcgatat
      661 acctgaaatg gttttgtaat tgtaagccaa acatgcagca actgttattt gtattaaaaa
      721 agccgcaaac tgttgcggct attttttttg tactgcttat tgccatgttg ggcaatattg
      781 gtttgcaaaa agcaattccg ttgcggccgc aaagcataca ccaatgggca caatgcgacc
      841 gtgcaagtgt tgcattaaat tatgcattaa aacgcactac cttttttaaa ccaatggtgc
      901 atgaatgcag taacggcagt ggcataagtg gcatggagtt gcctgtagtt aattacggtg
      961 cttctgtttt atatcagctt ttcggctatc atgaagcgta ttatcgtata tgggtaatgt
     1021 taattgccat tgccggaata gttgcagcat attatttggc gtgtttgttt ttaaatcagt
     1081 ggtgggcatt agccgctgtt ttacttttta tacaatcgcc cgtgctattg tactatatgc
     1141 ccaacttttt acccgatgca gcaagccttg gtttatcatt aatagcatgg tggctgtttt
     1201 ttaaaatcat cggacaaaaa tcaaacactt tacttctttg gtttttccta atcgtatcgt
     1261 tagccggctt aattaaagtt actgcgctta ttaatacaat aaccatgttg ttgtttgtag
     1321 taatacaaaa ccataaacgg ggttggcgtt cattgcttac taataatgta ctttgcgctt
     1381 tagttgttag cataattatt accggagcct ggtatattta tgcacggtgg ttaagcgtac
     1441 aataccatag ttattatttt ttaatggcgc cacattatcc aaccaactta gccgatgcct
     1501 ataagacatg gttgcgtatt aaaaccggca tgcttaacga gtattatacc gctgtattgg
     1561 gttatatatt attggccggc acaatagctt tagcttttaa ttttaaaaaa gtacaacggc
     1621 agttattatt tgtaactgtt acactatatg ctggcagctt tctttttttt atgcttatgc
     1681 ttaatcagtt cgtttatcac gattactact ttattgcttt aatgccggca gtgttatttc
     1741 atttaatgct tattgccaat ttggtgcaaa aattgaattt taaaatgcag cttggcattg
     1801 cattaataat cgttgtagca gcaaactatg gtatgaatat ttgtaaaaag aattttgcaa
     1861 gtcgttacaa tgagcaaagt tggtattaca ataatgcaaa atcaaaagcc tattttaatt
     1921 tcgaaactgt attacgcgaa aatggaattg catctgacga aaaaatttta tcgtttaccg
     1981 atgaaacacc taatacaagt ttgtacttaa tgaatcagcg tggcataagt cttggtgccg
     2041 atacaaacct ttggcatcct tatcttaagg ccggctataa atacgctgta ttaaacgata
     2101 gcttattgtt acactcaggt ttacaagctt ttttagataa aagacttacg tataaatttg
     2161 gattgtactt atacacatta aaaccacaac aatgatttgt gct
//
LOCUS       DAOMAA010000322         2203 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_47083_length_2203_cov_2.273277, whole genome shotgun sequence.
ACCESSION   DAOMAA010000322 DAOMAA010000000
VERSION     DAOMAA010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47083_length_2203_cov_2.273277"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="PLO59_05120"
     CDS             <1..331
                     /locus_tag="PLO59_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQV00520.1"
                     /translation="EMAEAKSFLESIARSFGYHYGVAKKVRINTISQSPTKTTAGGGI
                     KGFGAFYNYANEISPLGNASSQDCAKYCISLFSDYTRMVTMQNLFHDGGFSNTGVSHS
                     VMQKFED"
     gene            365..1123
                     /locus_tag="PLO59_05125"
     CDS             365..1123
                     /locus_tag="PLO59_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018966036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQV00521.1"
                     /translation="MPIVFITGATAGFGKAMAYQFAQRGYDIIINGRRADRLRVIEDD
                     LQNNFGVKVYALPFDVRNQTEVKAAIKSLPHAFLEIDVLINNAGLASGLATIQDGAIA
                     DWDKMIDTNIKGLLYVSHEIIPLMIARRKGHIVNIASIAGKEAYAKGNVYCATKHAVD
                     ALSKAMRIDLLPYGIKVTNIAPCAAETEFSIVRLHGDVEKAKQVYQGFEPLKADDIAE
                     VVYFAVSRPAHVVLNDIVITPKAQANTTYMIKKQ"
     gene            complement(1137..>2203)
                     /locus_tag="PLO59_05130"
     CDS             complement(1137..>2203)
                     /locus_tag="PLO59_05130"
                     /inference="COORDINATES: protein motif:HMM:NF024928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HQV00522.1"
                     /translation="TLFIIDEASLIGGAEIESETLFGRQSVLDDLIQYVYQGKNCIIM
                     FIGDMAQLPPVKLQLSPALDIANISFNYNMEVMSHELRDVVRQQKDSGILLNATALRI
                     LINKGLHLPVLKAVGFDDVFTLTGNEIGDVIAEKFNNKNPDDSVIICRSNKQANRYNQ
                     YLRNRVFDQFDELSAGDRLMILKNNYYWLDADSEVGFIANGDTIEIKAVLNIEDAFGF
                     RFADIRMALVDYPEAPYMEVKILLNTLYSETPALSYNDSKKLWDTLLKNHEKIKSNRA
                     RIEAVKSNAYYNALQVKFAYAITCHKSQGGQWPNVFVDQGFMTDESYTTEHLRWLYTA
                     VSRASSQLYYINFEDRFFKK"
BASE COUNT          682 a          418 c          422 g          681 t
ORIGIN      
        1 tgaaatggcc gaagccaaat cgtttttaga aagtattgcg cgcagttttg gttatcatta
       61 tggtgtggca aaaaaagtgc gcataaacac catctcacaa tcgccaacca aaacaacggc
      121 cggaggtggt attaagggtt ttggtgcctt ttataattac gccaacgaaa tttcgccttt
      181 aggtaatgca tcatcacagg attgtgccaa atattgtata tcgcttttta gtgattatac
      241 gcgcatggta accatgcaaa atttgtttca tgatggcggt ttttcaaata cgggcgtaag
      301 ccacagtgtg atgcaaaagt ttgaagatta ataaatttta ctgcttaatt aatttcaaat
      361 atttatgccc atagttttta taacaggtgc aacagcaggt tttggtaaag cgatggcata
      421 tcaatttgca caacgtggct acgatataat aattaatggc cgcagagctg atagattgcg
      481 cgtaattgaa gatgatttgc agaataattt tggtgttaaa gtatatgccc ttccttttga
      541 tgtacgcaat caaaccgaag taaaagcagc catcaaatca ttaccacacg cttttttaga
      601 aattgatgtt ttgattaata atgccggctt agcaagtgga ttagctacca tacaagatgg
      661 cgcaattgcc gattgggata aaatgattga taccaatata aaaggtttgc tttacgtaag
      721 tcatgaaatt attccattga tgatagctcg taggaaagga catattgtaa acatcgcatc
      781 aattgcaggt aaagaagcgt atgcaaaagg taatgtgtat tgcgcaacca aacatgctgt
      841 tgatgcctta agcaaagcta tgcgtattga tttattgcct tatggcatta aagttaccaa
      901 tattgcaccc tgtgctgccg aaaccgaatt ttcaatagta aggctacacg gtgatgttga
      961 aaaagcaaaa caagtatatc agggttttga accgctaaaa gctgatgata ttgccgaagt
     1021 agtatatttt gcagttagcc ggcccgcaca tgttgtgcta aacgatattg taattacgcc
     1081 caaagcgcaa gccaatacta cctacatgat taaaaagcaa tagccagaag caggctttac
     1141 tttttaaaaa accgatcttc gaaatttata taatacaact gtgacgatgc gcggcttacc
     1201 gcagtatata gccaacgcaa atgttcggtg gtgtagcttt catcggtcat aaatccctga
     1261 tcaacaaaaa catttggcca ttgcccgccc tgcgatttgt ggcaggttat tgcataggca
     1321 aattttactt gcagcgcatt atagtatgca ttgcttttta ccgcttcaat acgggcgcgg
     1381 ttgcttttaa ttttttcatg attttttagc aatgtatccc aaagcttttt gctatcgtta
     1441 tacgaaagtg caggtgtttc ggagtaaagc gtgtttagta aaatttttac ttccatgtaa
     1501 ggcgcctccg gataatcaac caatgccatt ctgatatcgg caaatctaaa accaaatgca
     1561 tcttcaatgt ttaatacggc tttaatttct attgtatcgc cattggcaat aaaaccaact
     1621 tcgctatcgg catctaacca gtaataatta ttcttcaaaa tcattaatcg gtcaccggcc
     1681 gatagttcat caaattgatc aaatacgcgg tttcttaaat actgattgta acggtttgct
     1741 tgcttgttcg aacggcaaat aattacggag tcatcgggat ttttattgtt aaacttttcg
     1801 gcaataacat caccaatttc attaccggtt agtgtaaata catcatcaaa accaacggct
     1861 tttaataccg gcaaatgtag gcctttattt atcagtatgc gtaatgctgt tgcattaagc
     1921 aatatgcccg aatctttttg ctgacgtact acatcgcgca attcatggct cattacttcc
     1981 atgttataat taaatgaaat atttgcaatg tccaaagccg ggcttaattg cagtttaaca
     2041 ggcggtagct gcgccatatc gccaataaac atgattatac agtttttacc ctgatacacg
     2101 tattgtatca aatcgtccaa taccgactga cgaccaaata aagtttctga ttcaatttcg
     2161 gcaccaccaa ttaacgatgc ttcgtcaatg ataaaaagtg tat
//
LOCUS       DAOMAA010000323         2199 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_47266_length_2199_cov_2.584422, whole genome shotgun sequence.
ACCESSION   DAOMAA010000323 DAOMAA010000000
VERSION     DAOMAA010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47266_length_2199_cov_2.584422"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..>2199)
                     /locus_tag="PLO59_05135"
     CDS             complement(70..>2199)
                     /locus_tag="PLO59_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00523.1"
                     /translation="IDYPFKIRYSINRGSMQTLDLSEGTNKQEENLNDQSKVEFTGIP
                     LYDSKLLAKGFIAETNEDLKMFLLYYGVTTHNYGKQTINIETAQAALSNNVFLAPYVS
                     GDLFESKDGKISDLAGVPQSGIFSTTAIGGLDVTTIADGLAKFLVKRTKQELNEAFFT
                     RLRDMLNDDKYRDLQALFPATWILLNNIGTEVYGFEKYIHSLRQAFKTDIDELLTHAS
                     VLVDNHPEYFETRKKEKASLLLALHIALSLQNDMHPADIIATLPMQPVLEADMGWHGA
                     LQTLQLLSFATRNVDGTSYWADINELRAVINDKERFRVFLGLCNQLALIKYNGIPYSD
                     NTNLYNTLNNLANEFDKHYAELSTWYLKFATHLNTLDNSLKAANTDQSDSARIAACLT
                     YFKAVVKCVEAGVDITKLTAFKNVPAIAEVAGKMKKPIEVSYTAVDLGQSLLAKNYAS
                     SVNHAIIIYELIFVKPLFAESNIAGAKKSAIDSTLESNISTLRKFAQVGTFMAEVSEA
                     NTSDEMAVVIENYALPSGSARIKKMSAWNVALAAYVGPYIGVERIKDVDPDLSDSEKE
                     INTFGLTAPVGISVSKGFGCKQRWSATLFASAIDIGAITAFRFKDSVTAQVPTIQLKD
                     IISPGLFLSFGIPKVPISLNIGIQCGPNLRQVVQDSLGVSNTYSDNLYVRYSASLCVD
                     IPLLNLYNKQPKLADGYRYASYKKSRK"
BASE COUNT          648 a          439 c          408 g          704 t
ORIGIN      
        1 atttaccata acatttaagt taaacaaatt aacatcagaa tgctttatac ttcgttaatc
       61 cacacatagt tactttcttg atttcttata ggaagcatat ctatagccat ctgctaactt
      121 aggttgctta ttgtataagt taagtaatgg aatatctaca cataatgaag cactgtatcg
      181 cacgtataag ttatcggaat aggtgttact aacccccaaa ctatcctgca ctacctgacg
      241 caagttaggc ccacactgta tgccaatgtt aagtgaaatg ggaactttgg gaataccaaa
      301 agataaaaac aaaccgggcg aaataatatc ttttaactgt atagtgggta cttgtgcagt
      361 tacgctgtct ttaaacctga atgcagtaat ggcgccaata tcaattgccg atgcaaatag
      421 ggttgcactc caacgttgtt tgcatccaaa acctttcgaa acacttatac ctacaggagc
      481 tgtaagtcca aatgtgttta tttctttttc ggaatcactt aaatcggggt caacatcttt
      541 aatgcgttct acacctatgt atggcccaac ataagcagcc aaagccacat tccaggcact
      601 cattttttta atacgagccg agccactggg taatgcataa ttttcgatta caacagccat
      661 ttcgtcactg gtatttgcct ccgatacttc ggccataaag gtgcctactt gcgcaaattt
      721 ccgaagtgtt gatatattgc tttctaaggt tgagtcaata gcacttttct tagcaccagc
      781 tatgttgctt tcagcaaaca gtggctttac aaaaataagt tcgtaaataa ttattgcatg
      841 atttacactt gaagcataat tttttgccag cagcgattgt cccaaatcaa cagccgtata
      901 acttacttcg atgggctttt tcattttgcc ggcaacttcg gcaatggcag gtacgttttt
      961 aaaggctgta agtttggtga tatcaacacc ggcttcaaca cattttacaa ccgctttaaa
     1021 ataggtaagg catgctgcaa tacgtgcact atctgattga tcagtatttg ctgcttttaa
     1081 actgttatct aatgtattta agtgtgtggc aaattttaag taccaggtac taagctctgc
     1141 ataatgttta tcaaattcgt tagcaaggtt atttaaggtg ttatataaat tggtattgtc
     1201 tgaatacgga atgccgttgt attttatcaa tgccaactga ttgcataaac ctaaaaaaac
     1261 acggaaacgt tctttgtcgt ttataactgc gcgcaattca tttatatcgg cccaatatga
     1321 agtaccatca acattgcgcg ttgcaaaact tagcaactgt aaggtttgca atgcgccatg
     1381 ccagcccata tcggcttcta atacgggttg catgggcagg gtggcaatta tatcggcagg
     1441 gtgcatatcg ttttgcagcg ataatgctat atgtaatgcc aacaataatg aggctttttc
     1501 ttttttgcgt gtttcaaaat actcggggtg gttatcaacc aataccgatg catgtgtaag
     1561 caactcatct atgtcggttt taaaagcctg gcgcaaactg tgtatgtatt tctcaaaacc
     1621 atacacttcg gtaccaatgt tattaagcaa aatccaggtg gccggaaaca aagcttgtaa
     1681 atccctgtac ttgtcatcat taagcatatc gcgcaagcgt gtaaaaaaag cttcgtttaa
     1741 ctcctgcttg gtgcgcttta ccaaaaattt ggcaagccca tcggcaatgg tggtaacatc
     1801 taggccgcct attgcagtag tggaaaaaat accggattgc ggaacacctg ctaaatcaga
     1861 tatcttgcca tccttacttt caaataaatc accactaaca taaggtgcga gaaaaacatt
     1921 gttgcttaat gcagcctgag ccgtttctat attaatagtt tgtttaccgt agttatgagt
     1981 tgtaacaccg tagtaaagta aaaacatttt taagtcttca ttagtttctg ctataaatcc
     2041 tttggctaac aacttagaat cgtacaaggg tatgccggta aattcgacct ttgattggtc
     2101 gtttaggttt tcttcttgct tgtttgtgcc ttcactcaga tctaatgttt gcatactacc
     2161 tctatttatg ctatatctta ttttaaacgg atagtcaat
//
LOCUS       DAOMAA010000324         2192 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_47547_length_2192_cov_2.056621, whole genome shotgun sequence.
ACCESSION   DAOMAA010000324 DAOMAA010000000
VERSION     DAOMAA010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47547_length_2192_cov_2.056621"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="PLO59_05140"
     CDS             complement(<1..381)
                     /locus_tag="PLO59_05140"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HQV00524.1"
                     /translation="MLKYNLIYLDSVDSTNTYALSLISKHEPPEGTIVYTYNQRQGKG
                     MQGNKWQSETGSNLLFSVILYPVFLASKNLFSLNQVIALAVCRSLEQLTMQRFKIKWP
                     NDIMHEDDKVCGILVENTFRGMHLG"
     gene            567..1658
                     /locus_tag="PLO59_05145"
     CDS             567..1658
                     /locus_tag="PLO59_05145"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HQV00525.1"
                     /translation="MIEVKDIKEVVPQTNLFSVLSNLGHEQVVFCQDKEAGLKAIIAI
                     HNTTLGPALGGTRMWMYNNESDALNDVLRLSRGMTYKASISGLNLGGGKAVIIGDSKK
                     DKTDKLFQRFGEFVNSLSGKYITAEDVGTSTNDMVNVSKSTKHVTGLPENQGGGGDPS
                     PVTAYGVFMGMKAAAKEMYGSDSLSGRKVSIQGCGHVGQNLVRLVTEDGGNVFVTDIH
                     QENLDKVKMYNPTIVDKDAVYDLDVDIYAPCALGATLNSVNIAKLKCSIVAGAANNQL
                     EDEVIHGQLLMQKGILYAPDFLINAGGLINVYSEIQGYNRDKAMQATQHIFNVTLDIF
                     KNSKANNIPTVDAAKHMAEKRISDAKASR"
     gene            1735..>2192
                     /locus_tag="PLO59_05150"
     CDS             1735..>2192
                     /locus_tag="PLO59_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00526.1"
                     /translation="MLSRRQLRVRVLQALYAFFQQENGDVWIAQKELLASAERVYDLY
                     LQLLNLLPEMAHHETMYIKDAPKKHIAKSNEIVNKLDENIIVKYLNSNLGFNDKLKKR
                     GLSWSKDTDLLSRIFLSFRMSDEYLQYISDKELTVKKQVDFVIHFYNQHI"
BASE COUNT          744 a          384 c          413 g          651 t
ORIGIN      
        1 gcccaagtgc atacctctaa acgtattttc aactaaaatg ccgcatactt tatcgtcttc
       61 atgcataata tcattaggcc atttaatttt aaaacgttgc atggttaatt gctctaaact
      121 tctacaaaca gccagtgcaa ttacctgatt aagtgaaaat aaattttttg atgccaaaaa
      181 cacgggataa agtataactg aaaaaagcaa attgcttccg gtttcgcttt gccacttatt
      241 accctgcata cccttaccct gcctttgatt ataagtgtaa acgatggttc cctcgggtgg
      301 ttcatgttta ctgattaacg acagggcata cgtgtttgta gaatctacag agtctaaata
      361 aataaggtta tactttaaca taatttgtat cggggtaatg ctgctaatat gctataaaat
      421 acacatatta aatgattaac ttccttatat tcaattaagc atcacaaaca atccaatcta
      481 attttgcatg gtattaaaaa aatctacctt tgcccgctca aaaattgttt aaaacatcct
      541 ttaatcccaa ctaaaaatat agcattatga ttgaagtaaa agacataaaa gaagtagtac
      601 ctcaaaccaa tttattttca gtattgtcaa atttaggcca cgagcaagta gttttttgtc
      661 aagataaaga agcaggctta aaagcaatta ttgctataca caacaccacc ttaggcccag
      721 ctttaggtgg aacccgtatg tggatgtata ataatgagag tgatgcgctt aatgatgtat
      781 tacgtttatc gcgtggtatg acctataagg cttcgataag cggcttaaat ttaggtggtg
      841 gtaaagcagt aattattggt gatagtaaaa aagacaaaac cgataaattg tttcaaagat
      901 ttggtgagtt tgtaaatagc cttagcggca aatatattac agccgaagat gttggcacaa
      961 gcaccaatga tatggttaat gtgagcaaat caaccaaaca cgtaaccggc ctacccgaaa
     1021 accaaggtgg cggtggtgat ccttcgcctg ttactgctta tggtgttttt atgggtatga
     1081 aagcagcggc aaaagaaatg tatggatctg atagcttatc gggccgtaaa gtttcgatac
     1141 agggttgtgg acatgtggga caaaacttgg ttcgcttagt taccgaggat ggcggtaatg
     1201 tttttgtaac cgatatacat caggaaaatt tggataaggt taaaatgtac aacccaacca
     1261 tagtagataa agatgccgta tatgatttag atgttgatat atatgcaccc tgcgctttag
     1321 gagccacttt aaacagtgta aatattgcca agttaaaatg cagtattgta gccggtgctg
     1381 ccaataatca attagaagat gaggtaatac atggccaatt gcttatgcaa aaaggtattt
     1441 tgtacgcacc cgatttttta attaatgccg gtggcttaat aaatgtttat agcgaaatac
     1501 aaggctacaa tcgcgacaaa gccatgcaag caacacaaca tatttttaat gtaaccttag
     1561 atatctttaa aaattcgaaa gcaaacaaca tcccaacagt tgatgctgcc aaacacatgg
     1621 ccgaaaaacg catcagtgat gccaaagcaa gccgttaatt ttaatgatta gtaagctaat
     1681 gctgttggtt tacaaaattg cttccaacaa cattatctga taaatattta tttcatgctc
     1741 tcacgaagac agttgcgcgt gcgcgttttg caagctttgt atgctttttt tcaacaagaa
     1801 aatggcgatg tatggattgc acaaaaagaa ttgctggcat cagcagaacg cgtttatgat
     1861 ttatacctgc aattgctgaa cctgctaccc gaaatggcgc atcatgaaac catgtacatt
     1921 aaagatgctc cgaaaaaaca tattgccaaa tcgaatgaaa ttgtaaacaa actcgatgaa
     1981 aacattattg taaagtatct taacagcaac ctgggtttta acgacaagct aaaaaaaagg
     2041 ggactgtcgt ggtcgaaaga tacggacttg ttaagtcgta tttttcttag ttttcggatg
     2101 agcgatgaat acctacaata tatatccgat aaagaattga ctgttaaaaa gcaagttgat
     2161 tttgtaattc acttttataa ccagcacatt tt
//
LOCUS       DAOMAA010000325         2180 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_48024_length_2180_cov_2.693647, whole genome shotgun sequence.
ACCESSION   DAOMAA010000325 DAOMAA010000000
VERSION     DAOMAA010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48024_length_2180_cov_2.693647"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1070
                     /locus_tag="PLO59_05155"
     CDS             <1..1070
                     /locus_tag="PLO59_05155"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HQV00527.1"
                     /translation="VNFCDPGMEHKEDLITGCNAAKAGGFTDVALMPSTIPAIQSKAQ
                     VDYILSKTQNHEVNVHPIGAVTLNTDGTELTEMYDMQQAGAVAFSNHKNTITNSGVMQ
                     RAMLYIKNINSTLLSFAEDKSLTGQNMVNESAQTTMLGFKGIPAIAEEIMVMRDIQLC
                     SYTQTPIHISRISTAGSVQLIAQARKNGLPVTCDVAAYNLYDTDAALQKFDSNYKVKP
                     PLRSAHDVAALIDGLVQGVIDIIVSDHTPQDVEAKKVEFDYAEFGMIGCESFFGIVFK
                     CLAEKMPLDKIIETFTTNPRKILKLPVYSIAENQPACLTLFSLDDYCFSNNHIQSKSN
                     NTPYIGSMLKGKAINIFTPGN"
     gene            1166..1360
                     /locus_tag="PLO59_05160"
     CDS             1166..1360
                     /locus_tag="PLO59_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011585718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HQV00528.1"
                     /translation="MKKGKVKFFNQSKGFGFIVDHENGQEVFVHSTGLKTEVRDGDEV
                     TFEITQGKKGLNAVNVERIA"
     gene            complement(1446..>2180)
                     /locus_tag="PLO59_05165"
     CDS             complement(1446..>2180)
                     /locus_tag="PLO59_05165"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HQV00529.1"
                     /translation="PIAPKILLKKVGFLSFGVDNILEMKTRQVTDTAVNLKKIKILEG
                     FSLNTGYNLAATQFKWSVLNMAARTTILEKFTLNGTAVFDPYAIDSSGLRINAFQYNN
                     NGNLFRFTNATVALGFSLNSVMFNDKDKTNSGYYKIPWNLTFSYSYNYSKPALTAVKT
                     QTLYCFGDFSMTERWRINFSSGWDFIAKDVSYTQLAIVRDLHCWEMRLSWVPFGLQQN
                     YFFTINVKAGMLRDLKLDKKRDQFDR"
BASE COUNT          747 a          398 c          394 g          641 t
ORIGIN      
        1 gtgtaaattt ttgtgacccc ggcatggagc ataaggaaga tttaattaca ggttgcaatg
       61 cggctaaagc aggcggtttt actgatgttg ccttaatgcc atcaaccata ccggcaatac
      121 aaagcaaagc acaagtagat tacatacttt cgaaaaccca aaaccacgaa gtaaatgtac
      181 accctatagg tgcagttact ttaaataccg atggcactga gttaaccgaa atgtatgata
      241 tgcagcaagc cggggctgtt gcatttagta accataaaaa tacgattaca aatagtggcg
      301 taatgcaacg tgctatgctg tatattaaaa atataaacag cacgctttta agttttgccg
      361 aagataaaag cttaaccggt cagaatatgg taaatgaaag tgcgcaaaca actatgcttg
      421 gctttaaagg catacctgcc attgccgaag agataatggt aatgcgcgat atacaattat
      481 gcagttatac acaaactccc atacacatta gcagaattag tacagccggc agtgttcaac
      541 taattgcaca agcgcgtaaa aatggtttgc ccgtaacgtg cgatgtagcg gcttataatt
      601 tatacgatac agatgcagcc ctgcaaaagt ttgatagtaa ttataaagta aaaccaccgt
      661 tacgcagtgc acatgatgta gctgcattaa ttgacggctt agtgcaaggt gtaatagata
      721 tcatagtgag cgaccatacg ccccaagatg ttgaggcaaa aaaagtagag tttgattatg
      781 ctgaatttgg catgatagga tgcgagtcgt tttttggtat tgttttcaaa tgtttggccg
      841 aaaaaatgcc tttagataaa atcatagaaa catttacaac taacccacga aaaattttaa
      901 agcttccggt ttattccatt gccgaaaatc aacccgcatg ccttacctta tttagtttag
      961 atgattattg tttttcgaac aaccatatac agtcgaaatc aaacaacacg ccttatatag
     1021 gttccatgtt aaagggcaag gcaataaata tttttacacc gggtaactaa gatgtttggc
     1081 tattagctga tacaattaaa aaaatgaggg gcgtatttta tttatactac atttagccca
     1141 ttcaataact attaaatttt ttaaaatgaa aaaaggaaaa gtaaaattct tcaaccaatc
     1201 aaaaggcttt ggttttattg ttgaccatga gaatggccaa gaagtgtttg ttcattcaac
     1261 cggcttaaaa acagaagtac gcgatggtga tgaagtaacc tttgaaatta cacaaggcaa
     1321 aaaaggatta aatgcagtaa atgttgaacg catcgcataa ataattttaa cttttgagtt
     1381 tcagtaacaa gggccgcaag gccctttttt tatgcaaatg cccaaacaca ggatttgtaa
     1441 aaaagttaac ggtcaaattg atctcgtttt ttatccaact tcaaatcacg taacatccct
     1501 gcttttacat ttatggtaaa aaaataattt tgttgtaaac caaaaggcac ccagcttaaa
     1561 cgcatttccc aacaatgcaa atcacgcaca attgctaatt gggtataact aacatccttt
     1621 gctataaaat cccatccgct gctaaaatta atacgccacc gttcagtcat actaaaatcg
     1681 ccaaagcaat ataatgtttg cgtttttaca gcggttaagg ccggtttgct ataattgtag
     1741 ctatagctaa aagtaagatt ccagggaatt ttataatagc ctgagttggt tttatcttta
     1801 tcgttaaaca taacactgtt taagctaaaa cctaatgcaa ctgtagcatt ggtaaaccta
     1861 aaaaggttgc cattattatt atattgaaac gcgttaattc taaggccgct gctatcaatt
     1921 gcataaggat caaaaacggc tgtcccattc aatgtaaact tttcgagaat agtggtacgc
     1981 gcagccatat ttaaaaccga ccatttaaac tgtgtagctg caaggttata acccgtattt
     2041 agcgaaaagc cttccaatat ttttatcttc tttaggttta cagccgtatc ggtaacctgc
     2101 ctggttttca tttcaagtat gttatcaaca ccaaaactta aaaaaccaac ttttttaagt
     2161 aaaatttttg gtgcaattgg
//
LOCUS       DAOMAA010000326         2178 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_48092_length_2178_cov_2.900612, whole genome shotgun sequence.
ACCESSION   DAOMAA010000326 DAOMAA010000000
VERSION     DAOMAA010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48092_length_2178_cov_2.900612"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            195..1082
                     /locus_tag="PLO59_05170"
     CDS             195..1082
                     /locus_tag="PLO59_05170"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HQV00530.1"
                     /translation="MVSSVEFWMLLVMCLCIIAEAAVSAFYKLGWYDGRDTFVNISLS
                     SLNFFLDLFFKGATFFVLTYFNQFAIINWNNSAFSWVILILLVDLAYYTLHWVDHNVR
                     FFWAIHVNHHSSEKMNVSVAIRSSVLQPLYRFIFFIPLALLGFSAVQILLVYLFINFW
                     GFFIHTEMIGRLGVLDKVFATPSNHRVHHGSNAHYLDRNMGMFLIVWDRLFGTYQAED
                     EKVVYGLTNNINTNRIDIVLVHEWIDMINDVKNAKGFYNKWMYVFGPPGWSHNGNKLT
                     SKQLRKYHFEVKQAALLKD"
     gene            complement(1111..>2178)
                     /locus_tag="PLO59_05175"
     CDS             complement(1111..>2178)
                     /locus_tag="PLO59_05175"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HQV00531.1"
                     /translation="NKYNPTQWADLIKQSGARYAVLTAKHHDGVALWDTKQQGLSVVK
                     NTPAKRDLIGPYIDALRNLNIKTGLYYSLIDWSHPDYDIFTRTQKRYTNDSLRWKKFT
                     GFYQAQLQELSTKYNPDLYWFDGDWEHTAAEWQADKVRKMLMYYNKNVIINSRLTNHG
                     DYATPEQGVPIQIPKDYYWELCYTMNDSWGYQPNDTHYKSCNQILNVLVDCIYKGGNL
                     LLDIGPKADGTIDEKQKQLLVDVGRWTKKHEAAVFGTLPGINTEYCMYPTALSADSNM
                     LYVFVSGKPASVYIKGLNNKVHRAYVLGQGTMLSYDIFGKAYWNKKPGMLSINLPQQL
                     CDNEITVIAILLDGPVSLYND"
BASE COUNT          624 a          351 c          405 g          798 t
ORIGIN      
        1 tggtgctgtt tcgtcttccg attttttttt gcaagcgcta ataattacta aggttgctaa
       61 acttagcgag agtagttttt tcattttttt ggggattaaa tttgtaatcg aaataagtta
      121 attttttttt actgagcaat tattttcaat ataaaagtca tatataaatt tacagcccta
      181 aaaaattata taaaatggta agttcagttg aattttggat gttgttagtt atgtgtttgt
      241 gcattattgc tgaggctgca gtaagcgcat tttacaaatt gggctggtac gatggccgcg
      301 atacttttgt aaacatcagt ttaagcagtt taaatttttt tctggatttg ttttttaaag
      361 gcgctacttt ttttgtgctt acatacttta atcagtttgc aataattaat tggaataaca
      421 gtgcttttag ttgggtcata ttgattctgt tagttgattt agcttattat acgttgcatt
      481 gggttgatca caatgtccgt tttttttggg ctattcatgt caatcatcat tcgtcagaaa
      541 aaatgaatgt ttctgtggcc attaggtcat ccgtgctgca accgctttat agatttattt
      601 tttttatacc actggcatta ttaggtttta gtgctgttca aatattattg gtttatctgt
      661 ttattaattt ttggggcttt tttattcata cagaaatgat tggtaggttg ggggtattag
      721 ataaagtttt tgctacgccc agcaatcacc gcgtacatca tggcagcaat gcacattatt
      781 tagacagaaa tatgggaatg tttttaattg tttgggatag attgtttgga acctatcagg
      841 ctgaagacga aaaggtagtt tatggcttaa ctaacaatat taatacgaac agaattgata
      901 ttgttttagt acatgaatgg attgatatga ttaacgatgt gaaaaatgca aaaggctttt
      961 acaataaatg gatgtatgta tttggaccac cgggttggag ccataatgga aacaaactaa
     1021 ccagtaaaca attacgtaaa taccattttg aagttaagca ggcagctctt ttaaaagatt
     1081 agatttatta tttcatctaa tgatagttag ctaatcgtta tacaatgaaa cagggccatc
     1141 taataatatg gctataacgg taatttcatt atcacataat tgctgtggta aatttataga
     1201 aagcatgccc ggttttttat tccaatatgc tttgccaaaa atatcatagc taagcatggt
     1261 tccttgcccc aaaacataag cacggtgtac tttgttattt aagcctttta tgtaaacgga
     1321 tgccggctta ccacttacaa aaacatatag catattgctg tcggctgata aggctgttgg
     1381 gtacatacaa tactctgtgt taatgccggg taatgtacca aatacggctg cttcatgctt
     1441 ttttgtccaa cgtccaacat ccactaacag ttgtttttgt ttttcatcta tagtaccatc
     1501 tgccttaggc ccaatatcta aaagtaagtt gcctccttta taaatacaat ctaccaatac
     1561 atttaaaatt tgattacaac ttttgtaatg ggtatcgttt ggctgataac cccagctatc
     1621 attcatagta taacaaagtt cccaatagta atctttaggt atctgaatgg gcacaccttg
     1681 ttcgggtgta gcataatcgc catggttggt tagtcggcta tttattatta catttttatt
     1741 gtaatacatc agcatctttc gcactttatc ggcttgccac tcggctgctg tatgctccca
     1801 atcgccatca aaccaatata ggtcggggtt gtacttggtt gataattcct gcaattgtgc
     1861 ctgataaaaa ccagtaaatt ttttccagcg caagctgtcg tttgtataac gtttttgggt
     1921 tcttgtaaaa atatcataat caggatgcga ccaatcaatt aacgagtaat acaagccggt
     1981 tttaatattt agattcctta aagcatctat atacgggcca attaaatcgc gtttggcagg
     2041 cgtatttttt acaacactta aaccttgttg tttggtgtcc cataacgcaa caccatcatg
     2101 gtgttttgcg gttaaaactg catatcgtgc gccactttgt ttaattaaat cggcccattg
     2161 agttgggttg tatttatt
//
LOCUS       DAOMAA010000327         2177 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_48133_length_2177_cov_2.919887, whole genome shotgun sequence.
ACCESSION   DAOMAA010000327 DAOMAA010000000
VERSION     DAOMAA010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48133_length_2177_cov_2.919887"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="PLO59_05180"
     CDS             <1..287
                     /locus_tag="PLO59_05180"
                     /inference="COORDINATES: protein motif:HMM:NF018663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQV00532.1"
                     /translation="NKIMHIALEAPNEIRLMGNDFVDFTGEPFVAGNNFSLSLHPDSE
                     ELADKLFNNLSVEGNIIVPISKAPWGDYFGMFIDKFGINWMVSFDETPQQ"
     gene            369..827
                     /gene="rnhA"
                     /locus_tag="PLO59_05185"
     CDS             369..827
                     /gene="rnhA"
                     /locus_tag="PLO59_05185"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HQV00533.1"
                     /translation="MITIYTDGSSRGNPGPGGYGIVMMSATHQKEISEGYRYTTNNRM
                     ELLSVIVALETLKKENTIVTVYSDSKYVVDAIEKKWVFGWEKKNFAGKKNPDLWQRFL
                     KIYRQHRVAFKWVKGHATNPFNNRCDSLATTAALGQNLLVDFAYESELKQ"
     gene            complement(847..>2177)
                     /locus_tag="PLO59_05190"
     CDS             complement(847..>2177)
                     /locus_tag="PLO59_05190"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HQV00534.1"
                     /translation="AFFEALRDFLNTHKFTAVEAHQLRLSFEKITGEDLNWFWNQWYY
                     NKGYPILNIDYLYDATTQTAQVIVNQKTSDKSVAFFKLPVAIDFYLNGKVVRKQFTIT
                     GETDTFKYVTATQPDLINFDAEKMLVCKKTDNHTNAEWLYMLQHAPLYLDRFEALTKI
                     GNAKTGTAEAKTLINKASTDPYYNTRIKATNGLRELAASADNKDAVKNLLLQAATDNK
                     SAIRSNAIEKLSNWYDDDFTSLYKKAITTDSSYMVIGSALNALTTKNPSEGLAMAESL
                     EHQNDENINSIIAKTYSNKGTAKHATFMINHLQNSKGFETFGAINQMNTYLKSQDENT
                     IEKCVPAYESIATSQRSWFIKLQATNQLSSLGTSVQLKEKDVESEIEKAKTENKSASE
                     IATLEQKRDNLKRLESSIKSKVEKIKSSETDERLKKLYSGDGDVEINVQD"
BASE COUNT          630 a          363 c          404 g          780 t
ORIGIN      
        1 aaaataaaat tatgcatatt gcattagaag ctcctaacga aattagatta atggggaatg
       61 attttgttga ttttactgga gaaccttttg ttgcaggaaa taatttttca ttatcattac
      121 acccagatag tgaagaatta gcagataaat tatttaataa tctttctgta gaaggaaata
      181 ttattgttcc aataagtaaa gctccttggg gagattattt tggaatgttt attgataagt
      241 ttggtattaa ctggatggtg agctttgacg aaacacctca gcaataattt gagtgggcat
      301 aaattcaaaa gcgcttaggt taaaaaaaac taagcgcttt ttttgttatt tgttgcctat
      361 ttttaaagat gataacaatt tataccgatg gttcatcgcg tggcaacccg gggccgggtg
      421 gttatggtat tgtgatgatg agtgcaacgc accaaaaaga aatttcggaa ggttatagat
      481 acaccacaaa taacagaatg gagttactaa gcgttattgt ggcattagaa accttaaaaa
      541 aagaaaatac aattgtaacc gtttacagcg attcgaaata tgttgttgat gccatcgaaa
      601 aaaaatgggt gtttggttgg gagaaaaaaa attttgcagg caaaaaaaat cctgatctat
      661 ggcaacgttt tttaaaaata taccgtcagc atcgggttgc atttaaatgg gttaaaggcc
      721 atgctaccaa ccctttcaat aaccgttgcg atagtttagc tacaacggct gcgctcggac
      781 aaaacctatt ggttgatttc gcatacgaaa gtgaattaaa acaataagca ttcagtgctt
      841 attgttttaa tcctgcacat ttatctctac atcgccatca ccactgtata gctttttaag
      901 tctttcatcg gtttcgctac ttttaatttt ctcaaccttt gatttgatac ttgattcaag
      961 cctttttaag ttatccctct tttgttcaag tgttgcgatt tctgatgctg atttgttttc
     1021 ggttttggct ttttcaattt cactttcaac atctttttct tttaattgaa cagatgtacc
     1081 taaactagat agctgatttg tagcttgcaa ttttataaac caactacgtt ggctggttgc
     1141 aatagattcg taggccggca cacatttttc aatcgtgttt tcatcctgac tttttaaata
     1201 ggtattcatc tgatttattg cgccaaaagt ttcgaaaccc ttactgtttt gtaaatgatt
     1261 aatcataaat gttgcatgct ttgctgtgcc tttattgcta taggtttttg caataatgct
     1321 attaatgttt tcatcgtttt gatgttctaa actttcggcc atggctaaac cctcgctcgg
     1381 gtttttggtg gtaagtgcat ttaaagcact gccaattacc atgtacgaac tgtcggtggt
     1441 gatggctttc ttatataaac tggtaaaatc atcatcatac cagttgctta atttttcgat
     1501 ggcattacta cgtatagctg atttgttatc ggttgcagct tgcaacaaca aatttttaac
     1561 cgcatcttta ttatcggctg acgctgctaa ttctcttaaa ccattggtag ccttgatacg
     1621 ggtattgtaa tatggatcgg ttgaagcttt attaataagt gtttttgctt cggctgtgcc
     1681 tgtctttgca ttaccaatct tggttagtgc ttcaaaccgg tcgagatata agggtgcatg
     1741 ttgcagcata tataaccatt ccgcatttgt atgattgtcg gtttttttgc agaccagcat
     1801 tttttcggca tcaaaattta ttaggtcagg ttgtgtagct gttacatatt tgaatgtatc
     1861 ggtttcaccg gtaattgtaa actgtttacg aaccacttta ccgttaaggt aaaaatcaat
     1921 tgctaccggc aatttaaaaa aggcaacact tttatcgctg gttttttgat ttacaataac
     1981 ctgggcagtt tgtgttgttg catcatacaa atagtcaata tttaaaatcg gatacccttt
     2041 attgtaatac cattgattcc aaaaccagtt taaatcttcg cctgtaattt tttcgaaact
     2101 taatcttaac tgatgggctt caactgctgt aaatttatgc gtatttaaaa agtcacgtaa
     2161 ggcttcaaaa aaagctt
//
LOCUS       DAOMAA010000328         2175 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_48212_length_2175_cov_3.216038, whole genome shotgun sequence.
ACCESSION   DAOMAA010000328 DAOMAA010000000
VERSION     DAOMAA010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48212_length_2175_cov_3.216038"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="PLO59_05195"
     CDS             <1..328
                     /locus_tag="PLO59_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013665006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HQV00535.1"
                     /translation="LAGAEMARTEQYKEGRVPLHTFRADIDYAVSEAQTSYGKIGVKV
                     WICRGEVYGKRDLSPNIGLNTQTKVGSERNEGRGERKERGEGRGSRGTGRSGDNRGRA
                     PKKRDE"
     gene            375..794
                     /gene="rplP"
                     /locus_tag="PLO59_05200"
     CDS             375..794
                     /gene="rplP"
                     /locus_tag="PLO59_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008203023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HQV00536.1"
                     /translation="MLQPKKTKFRKQHKMKAKGNATRGADIAFGSFGLKSLETAWITG
                     RQLEAARIALTRYMKREGQSWIRVFPDKPITKKPAEVRMGKGKGAPEYWVAVIKPGTI
                     MFEAEGVPLNVAKEAMRLASQKLPVLTRFIVRRDYVE"
     gene            830..1024
                     /gene="rpmC"
                     /locus_tag="PLO59_05205"
     CDS             830..1024
                     /gene="rpmC"
                     /locus_tag="PLO59_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HQV00537.1"
                     /translation="MKQNIVKDLTNDELRDKLAEERSAYTKIKITHQVSPLENPMKIR
                     AMRRTVARLKTEMVNRQLSK"
     gene            1033..1287
                     /gene="rpsQ"
                     /locus_tag="PLO59_05210"
     CDS             1033..1287
                     /gene="rpsQ"
                     /locus_tag="PLO59_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HQV00538.1"
                     /translation="MERSLRKEKIGIVTSDKMAKSIVVSIERKEKHPIYGKFIKKTKK
                     FMAHDEKNEAHVGDKVRIMETRPLSKNKNWRLVDVVEKAK"
     gene            1338..1706
                     /gene="rplN"
                     /locus_tag="PLO59_05215"
     CDS             1338..1706
                     /gene="rplN"
                     /locus_tag="PLO59_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002675043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HQV00539.1"
                     /translation="MIQQESRLTVADNSGAKEVLCIRVLGGTKKRYASIGDKIVVTVK
                     HAIPSGNVKKGTVSKAVVVRVSKEVRRKDGSYIRFDDNAVVLLNANDELRGTRIFGPV
                     ARELREKEFMKIVSLAPEVL"
     gene            1709..2044
                     /gene="rplX"
                     /locus_tag="PLO59_05220"
     CDS             1709..2044
                     /gene="rplX"
                     /locus_tag="PLO59_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HQV00540.1"
                     /translation="MEKKVKLKIRKGDMVIIMSGESKGKQGKVLSVVKESNRAIVEGV
                     NQVSKHSKPNAKNTQGGIVKQEASIHMSNMMLVDPKTGKPTRVGRKVEDGKIVRYAKK
                     SGDLIKENK"
BASE COUNT          837 a          332 c          488 g          518 t
ORIGIN      
        1 tttagctggt gctgagatgg cacgtactga acaatacaaa gaaggtcgtg tgcctttgca
       61 cacattcaga gctgatattg attatgcggt atctgaagca caaaccagct atgggaaaat
      121 tggtgttaaa gtatggattt gtcgtggcga agtttatggc aagcgtgatt tatctccaaa
      181 tatcggatta aatacacaaa cgaaagtagg tagcgaacgt aacgaaggtc gtggagagcg
      241 taaagagcgt ggcgaaggcc gcggaagcag aggtactggg cgcagtggtg ataacagagg
      301 acgcgcacca aaaaagagag atgaataaaa cggatcaagt aaaataataa ttagaaattt
      361 aaaaatatta ataaatgtta cagccaaaga agactaaatt cagaaagcag cataagatga
      421 aagcaaaggg taatgctacc cgtggtgctg atattgcttt cggatctttt ggtttgaagt
      481 cattagaaac ggcatggatt acaggccgcc agttagaagc agctcgtatt gcacttaccc
      541 gatatatgaa acgtgaaggc cagtcttgga tccgcgtttt tcctgacaag cctatcacta
      601 aaaaacctgc agaggtacgt atgggtaaag gtaaaggtgc accagagtat tgggttgcag
      661 ttattaaacc gggtaccatt atgtttgaag cagaaggcgt tccattaaat gtagctaaag
      721 aagcaatgcg ccttgccagc caaaaactac cggtattaac cagatttatt gtaagacgcg
      781 attacgtaga gtaagcgtta aaaagtaaaa tttataaaaa agatttacaa tgaaacagaa
      841 tatagtaaaa gatttgacga atgacgaact gcgcgataag ttagcagaag aacgttcggc
      901 ttatacaaaa attaaaataa cacatcaggt ttctccatta gaaaacccga tgaaaatacg
      961 tgccatgcgc agaacagtag cacgcttaaa aacagaaatg gttaaccgtc aattatcaaa
     1021 ataaatttac acatggaaag gtcacttcga aaagaaaaaa tcggtatcgt aacgagcgat
     1081 aaaatggcta aatcaattgt ggtatcaatt gaacgcaaag aaaagcatcc gatttatggt
     1141 aagtttatta aaaaaaccaa aaaatttatg gcgcatgacg aaaaaaatga agcgcatgta
     1201 ggtgataagg tacgcattat ggaaacacgc ccattaagca aaaacaagaa ttggcgtttg
     1261 gttgatgttg tagaaaaggc aaaataatcg aacctattaa agaaattatt tttttgaaaa
     1321 aatatcaaga ataaaaaatg atacaacaag agtcgaggct aaccgtagcc gataatagtg
     1381 gagcaaaaga agtgctatgt attcgtgtat tgggtggtac caaaaagcga tatgctagca
     1441 taggcgataa aattgtagtt accgtaaaac atgcaattcc atccggtaat gttaaaaaag
     1501 gtaccgtttc aaaagctgtt gtagtacgtg taagtaagga agtgagacga aaagacggct
     1561 catacatcag gtttgacgac aatgcagttg tgttattaaa tgcaaatgat gaattacgtg
     1621 gtacacgtat ttttggccca gttgcacgag aattgcgcga aaaagaattt atgaaaatag
     1681 tttcattagc accggaggta ctataattat ggagaagaaa gtaaaactaa aaataagaaa
     1741 aggcgacatg gtgattatta tgtcgggcga atcaaaaggt aaacaaggta aagtattatc
     1801 tgttgtaaaa gaatcaaacc gggcaattgt tgaaggcgtt aaccaggttt cgaaacatag
     1861 caaacctaat gctaaaaata cccaaggtgg tattgtaaag caagaggcca gtatccatat
     1921 gagcaacatg atgttggtag accccaaaac cggtaaacca acacgtgtag gccgtaaagt
     1981 tgaagatgga aaaattgtgc gttacgcaaa aaaatcaggc gatctgataa aggaaaacaa
     2041 gtaataagaa aaagttttag ttgctacatg taacagactt aaaaatatta ataacatggc
     2101 ttacagtcca cgattaaaag ataaatataa aaacgagata gcaaaaagca tgatgacacg
     2161 ctttagctac aaaag
//
LOCUS       DAOMAA010000329         2175 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_48220_length_2175_cov_2.663679, whole genome shotgun sequence.
ACCESSION   DAOMAA010000329 DAOMAA010000000
VERSION     DAOMAA010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48220_length_2175_cov_2.663679"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="PLO59_05225"
     CDS             complement(<1..180)
                     /locus_tag="PLO59_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013929251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HQV00541.1"
                     /translation="MNDIKETVKAILTDYLKHNELRKTPERFAILEAIYDTDVHFDVD
                     WLYNEMKRKKFTLSRA"
     gene            complement(190..>537)
                     /locus_tag="PLO59_05230"
     CDS             complement(190..>537)
                     /locus_tag="PLO59_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HQV00542.1"
                     /translation="INEGIKIHRVNCPNAIQLMSNYGYRIVKAKWTHQKEIAFLAGIK
                     INGTDDVGVVNNITKVISNELKVNMRSISIDSNEGLFEGTIMLFVHDTKHLDKLIAKL
                     LKVKGIYSISRIE"
     assembly_gap    538..547
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<548..>2175)
                     /locus_tag="PLO59_05235"
     CDS             complement(<548..>2175)
                     /locus_tag="PLO59_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017259126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HQV00543.1"
                     /translation="GLGTTSIVCALLHDTVEDTHITLEDIAQQFGAKTAKIIDGLTKI
                     SGVFDNSSSIQAENFRKMLLTLSDDVRVILIKLADRLHNMRTLGSMPRDKQLKIASET
                     AYLYAPLAHRLGLYAIKTEMDDLVLKYKEPQVYKDIVKQLKQTKREREAFIAEFMQPI
                     RKSLDAHGLKYEIKGRTKSIHSIFNKMKKQNIPFEEVFDLFAIRVILDSEQDREKPDC
                     WQAYSIVTDYYKPNPDRLRDWISTPKANGYESLHTTVMSTGGRWVEVQIRSLRMDEIA
                     EKGYAAHWKYKESSSESAVDEWINRIREMLENSEGNALDFIDDFKLNLFSDEIFVFTP
                     KGDLRTLPVGATALDFAFEIHTNIGEKCIGAKINHKLVPLSHVLNSGDQIEILTSSKQ
                     NPKEDWLEFAITAKAKSKIKSALKEEKRKISEDGREIFDRKFKNLKLDKSDFDMHKLL
                     MYLRLPNASEFYYRLAKEVIDFSEVKDFLINKDRIDEKLASRPETKSLEDVVKQIRGS
                     NEALVIGDNLTNMDYKLSPCCNPISGDDVFGFIT"
BASE COUNT          577 a          423 c          391 g          774 t
ORIGIN      
        1 agcacgactg agagtaaatt tcttacgttt catttcattg tataaccagt caacatcaaa
       61 atgtacatct gtatcataaa ttgcttccaa aatggcaaaa cgttcggggg ttttacgcag
      121 ttcgttatgt ttcaggtaat cggttaaaat ggcttttaca gtttctttta tatcattcat
      181 tttggtttgt tattcaatac gtgaaatact ataaattcct tttaccttta ataatttggc
      241 aatcagttta tcaagatgtt tggtatcatg cacaaacaac atgatggtgc cttcaaataa
      301 tccttcatta ctgtcaatac taatgctgcg catatttact ttcaattcat tactgataac
      361 tttagtaatg ttgtttacaa cgccaacatc gtcagtacca ttaattttaa tgcctgccaa
      421 aaatgcaatt tctttttgat gtgtccattt tgcttttact attctatagc catagtttga
      481 catgagttgt atggcattag gacaatttac acggtgaatt tttatacctt catttatnnn
      541 nnnnnnnata gttataaagc caaacacgtc atcgcccgat atgggattac aacaaggcga
      601 aagtttataa tccatattgg ttaaattatc accaataacc aatgcttcgt tgctgcctct
      661 tatttgtttt acaacatctt caagcgattt ggtttcgggc ctgcttgcaa gcttttcatc
      721 tatccggtct ttgttgatta agaagtcttt aacctcggaa aaatcaatta cttctttggc
      781 taacctgtaa taaaattcag atgcattagg caagcgtaag tacatgagca atttatgcat
      841 atcaaaatcg ctcttgtcta actttaggtt tttgaatttg cggtcaaaaa tttcgcggcc
      901 gtcttcactt atctttcgtt tttcttcttt taaagccgac ttaatttttg atttggcttt
      961 tgcagtaatt gcaaactcaa gccagtcttc ttttggattt tgcttggatg aggtaagtat
     1021 ttcaatttgg tcgccactgt taagtacatg actaagtggt accagtttgt gatttatttt
     1081 ggcaccgata catttttcgc caatgttggt atgtatttcg aaagcaaagt ctaaagcagt
     1141 tgcaccaacg ggtagtgtgc gtaagtcgcc ttttggtgtg aatacaaaaa tttcatcgct
     1201 aaaaaggttt agtttaaaat cgtcaataaa atcgagtgca ttgccttctg agttttcgag
     1261 catttcgcga atgcggttta tccattcatc aacggcactt tcgctgctcg attctttata
     1321 cttccaatgt gctgcatagc ctttttcggc aatttcgtcc atacgtaatg aacgtatctg
     1381 cacttcgacc caccgcccgc ctgtgctcat tactgtggta tgcaatgact catatccatt
     1441 agctttaggt gtacttatcc aatcgcgtaa acgatcgggg ttgggtttat aataatcggt
     1501 aacaatgctg taagcctgcc agcaatctgg tttttcacgg tcttgttccg aatctaaaat
     1561 cacacgaatt gcaaacagat caaaaacctc ttcaaaaggt atgttctgtt ttttcatttt
     1621 attgaagata gagtgaatgc ttttggtgcg cccttttatc tcatacttta aaccatgtgc
     1681 atctagcgat ttgcgtatgg gttgcataaa ctctgcaata aaagcttcgc gctcgcgctt
     1741 ggtttgtttt aattgtttta caatgtcttt atatacctga ggctctttat acttcaacac
     1801 caaatcgtcc atttcggttt taattgcata caaacccaaa cggtgtgcaa ggggtgcata
     1861 gaggtaagct gtttcggatg caatttttaa ctgcttatcg cgaggcatgc tacctaaggt
     1921 gcgcatgttg tgtaacctat cagccagttt tataagtata acgcgcacat catcactgag
     1981 tgttagcaac atctttctaa aattttcggc ttgtatgctg ctgctgttat caaacacacc
     2041 tgatattttg gttaggccat ctataatttt tgctgtcttg gctccgaact gttgggctat
     2101 atcttctaaa gttatgtgcg tatcttcaac ggtatcatgc agcaatgcac atactatgga
     2161 ggtggtgcct aaacc
//
LOCUS       DAOMAA010000330         2175 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_48228_length_2175_cov_2.318396, whole genome shotgun sequence.
ACCESSION   DAOMAA010000330 DAOMAA010000000
VERSION     DAOMAA010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48228_length_2175_cov_2.318396"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="PLO59_05240"
     CDS             complement(<1..746)
                     /locus_tag="PLO59_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00544.1"
                     /translation="MKKLLFTLSFFLTTITVFSQNCYFSEDMEVIDSVTSTGTPGFNA
                     NTTYPHTGLQSTLGTYAQGNEAVLTTVDIDFATPGHSFVLLKFWHICKLEFFDQGIVQ
                     VSNDGGTTWTNLTATEYMGSAPFGQQGNKFVESSYPTWQPGNAGALPDSTWWKFETFD
                     ISALVGLSPNAKVRWVAKDLNNSGMGGRLGWLIDDICIESAPCEVFPPVVTQLPPVLS
                     NTIYNLGPFDLNLNVTDQSGLAAVYIFYTV"
     gene            complement(912..1784)
                     /gene="fabD"
                     /locus_tag="PLO59_05245"
     CDS             complement(912..1784)
                     /gene="fabD"
                     /locus_tag="PLO59_05245"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014796556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HQV00545.1"
                     /translation="MKAYIFPGQGSQFVGMGNDLYEQNISARQLFETGNEILGFRITD
                     LMFNGTDEDLKQTNVTQPAIFLHSVVQAKVLENNFMPDMVAGHSLGEFSALVAIEALS
                     FESGLKLVMERAQAMQEACLKNPSTMAAVLGLADEVVETICNQINDVVVCANFNCPGQ
                     LVISGSISGIDTACKMLTEAGAKRALKLAVGGAFHSPLMEPARERLAMAINQTHFNIP
                     RCNIYQNVDARPYSNPDEIKTNLIAQLTAPVRWTQTINNMIADGATSFTEIGPGKVLQ
                     GLVKKINKVAVIVS"
     gene            complement(1880..>2175)
                     /locus_tag="PLO59_05250"
     CDS             complement(1880..>2175)
                     /locus_tag="PLO59_05250"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HQV00546.1"
                     /translation="YIPNGRIVGLSKIPRVVDAFSRRLQVQERLTNEIRDCIQSTLTP
                     LGVAVVIEAQHLCMQMRGVQKQNSVTTTSAFTGEFENDKTRSEFISLIKTYTR"
BASE COUNT          669 a          426 c          405 g          675 t
ORIGIN      
        1 ttaacggtat aaaaaatgta aacagcagcc aatcctgatt ggtcggtaac atttaaattt
       61 aagtcgaatg ggcctaagtt ataaattgtg ttcgataaaa caggtggcaa ttgagtaacc
      121 acaggcggga atacttcaca tggtgctgat tcaatacata tatcatcaat caaccagcct
      181 aaacgaccac ccatgcctga gttatttaag tcctttgcaa cccaacgaac tttagcattg
      241 ggacttaatc ccactaatgc cgaaatatcg aacgtttcga atttccacca ggtagaatcg
      301 ggtaaggcac ctgcattacc gggttgccaa gtagggtatg atgattcgac aaacttattt
      361 ccttgctgac caaaaggtgc cgatcccata tactctgtgg ctgtaagatt tgtccatgta
      421 gtaccaccat cgtttgaaac ctgaacaata ccttggtcaa aaaactcgag cttacagata
      481 tgccaaaact taagtaacac aaaactgtga cccggtgttg caaaatcaat atcaaccgta
      541 gtaagcaccg cttcgttgcc ttgagcataa gtacccaaag tactttgtaa acctgtatgc
      601 ggataggttg tgtttgcatt aaaacccggt gtaccggttg aggttaccga gtcaataact
      661 tccatatcct cagaaaaata acagttttga ctaaaaaccg ttattgtggt taagaagaaa
      721 gacagcgtaa atagtagttt tttcatgggc aatttgattt gaatttcttt ggtgttgtat
      781 ttctgttact aagtatgagg ataaaataaa ggcgtaaaaa tatattttaa attgaaataa
      841 gtataacagt tgtgtatgta tgaagtcaaa ctatttaaaa tagtttcatt ttttagggtt
      901 aggtatgcat attaacttac aataactgcc actttattta tttttttaac caaaccttgt
      961 aaaactttac caggcccaat ttcagtaaaa ctggttgcgc catcagcaat catgttattg
     1021 atggtttgag tccatcgtac cggtgctgta agctgcgcta ttaaattggt tttaatttca
     1081 tcaggattcg aatatggccg ggcatcaaca ttctgataga tattacacct tggtatatta
     1141 aagtgtgttt gatttattgc cattgctaac ctttcgcgtg caggttccat taatggtgag
     1201 tgaaatgcgc cacctacagc caattttaac gcacgtttgg caccggcttc ggtaagcatt
     1261 ttacaagccg tatctatacc ggaaatgctg cctgaaataa ctaactgacc ggggcaatta
     1321 aaattagcac atactaccac atcgttaatt tgattacaaa tggtttctac tacttcatcg
     1381 gccaaaccaa gtaccgctgc catcgttgag ggatttttta aacaagcttc ctgcatggct
     1441 tgcgcacgtt ccataaccaa ttttaatccg ctttcgaatg ataatgcttc gatagcaacc
     1501 aaggccgaaa actcgcctaa tgaatgacct gcaaccatat cgggcataaa attgttttct
     1561 aaaactttgg cttgtactac actatgcaaa aaaatggcag gttgtgtaac attggtttgc
     1621 ttcaagtcct catcagtacc gttaaacatt aaatcggtaa ttctgaatcc taaaatttca
     1681 ttaccggttt cgaatagttg ccgggccgaa atgttttgct cgtacaaatc attacccatt
     1741 cccacaaatt gagaaccttg acccggaaaa atatatgctt tcataagttg cgtgtaactt
     1801 taatttgaat tgggctaatg taaaaaaagt gtttaatggc tgagttggat ttataataaa
     1861 gtatgcattt gaaatatgcc tacctcgtat aggttttaat taaacttata aattcactac
     1921 gtgttttgtc gttttcaaat tcgcctgtaa atgccgaagt agtagtaacc gaattttgtt
     1981 tttgtacacc acgcatttgc atacataaat gttgtgcctc aataactaca gctacaccca
     2041 gtggcgttag tgtactttga atacaatcgc gtatttcgtt ggttaatcgt tcctgcactt
     2101 gaagcctgcg ggaaaatgca tctacaacac ggggaatttt actcaatcca acaatacgcc
     2161 cattaggaat gtatg
//
LOCUS       DAOMAA010000331         2174 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_48275_length_2174_cov_2.588485, whole genome shotgun sequence.
ACCESSION   DAOMAA010000331 DAOMAA010000000
VERSION     DAOMAA010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48275_length_2174_cov_2.588485"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..924)
                     /locus_tag="PLO59_05255"
     CDS             complement(43..924)
                     /locus_tag="PLO59_05255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQV00547.1"
                     /translation="MITVIIPTYNYARFLPDAINSVLAQTYSNWKLIIIDSSSTDNTA
                     EVLKPFLNDVRITYQVVEKIGVSYARNVALAQVETPFIQFLDADDWIEPQKFELMLQA
                     FTDEIDLIYSEARYFRNGFNTLLYSMHLPDKKWMPCCSGALVLNYLIRNNIMPINAPI
                     FRTDLIRKFGFFDTTLTGLEDWELWLRFAINGSNFKCIEAGNIAAVVRIHATSASTDL
                     RMMHKHILPVLYRYKKSSGLNLRQKLYLNVRCFESFWDGVFAKNTSDVVTINTTLKMI
                     WIFMLPVYLILKLIRFI"
     gene            complement(921..1628)
                     /locus_tag="PLO59_05260"
     CDS             complement(921..1628)
                     /locus_tag="PLO59_05260"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQV00548.1"
                     /translation="MLISIVTINRNNAGGLFQTVKSVFSQNYAAIEYIIIDGASADNS
                     LELIMPYRAKINHVISEPDNGIYDAMNKGLHKATGTYIIFLNSGDIFCSDDVVRNVVR
                     LKATEDLIYGNLFFSDKIATERKQPAYLTARHMLRDTIWHPATFYRTELLKACNGYNI
                     DYTIAADYELLLRLIYKHNATTRYINMPVAVFDVTGISGKPQMQQTLFKQRHQAQRNN
                     MPLYYWLPYQLYSTLRK"
     gene            complement(1622..>2174)
                     /locus_tag="PLO59_05265"
     CDS             complement(1622..>2174)
                     /locus_tag="PLO59_05265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQV00549.1"
                     /translation="QQFSYKPLIDSRNKLNLPPNETVVLFVANDVNNMRKGIQFIIVA
                     ANKSKHKFYGIGKPDANLPSCINQLGYLTPEELQWWYVAANVFVLPSLAENFPNTICE
                     SLCCGTPVVAFNTGGIPELIDNNNGVLIPDFNATQLLMAIDNCIANNQHFNRKQIASD
                     ADKKLSSTTVLQMHITLYQSVLC"
BASE COUNT          707 a          407 c          363 g          697 t
ORIGIN      
        1 acgttataac ttacaattac tactgaaact gtatgcatac tatcatataa atcgaattag
       61 ttttagtatc aaataaacag gcaacataaa aatccaaatc atttttaaag ttgtgtttat
      121 tgttaccaca tcacttgtgt tttttgcaaa aacgccatcc caaaacgact caaaacacct
      181 tacatttaaa tacaattttt gcctaagatt taatccactg ctttttttat atcgatataa
      241 tacgggcaat atatgtttat gcatcatacg taaatcagtg ctggcacttg ttgcatgaat
      301 acgaaccact gcagcaatat taccggcttc aatacactta aaattgctac cattaattgc
      361 aaatcgtaac cataactccc aatcttcgag gccggttaag gtggtatcaa aaaaaccaaa
      421 cttgcgtatt aaatcagttc gaaatatggg cgcattaatg ggcataatat tatttctgat
      481 cagatagttt aataccaaag cgccactaca acaaggcatc cattttttat caggtaaatg
      541 catagaatat aaaagcgtat tgaaaccgtt tcgaaaatac ctggcttcgc tgtaaattaa
      601 gtctatttca tccgtaaacg cctgcaacat aagttcaaac ttttgaggtt caatccaatc
      661 atcggcatct aagaactgaa taaaaggtgt ttcaacctgg gccaaggcta cattacgtgc
      721 ataacttact ccaatttttt ctacaacctg ataggtgata cgaacatcat ttaaaaacgg
      781 cttcaatact tcggcagtat tatcggtaga actactgtca ataattatca gtttccaatt
      841 actataagtc tgcgctaaaa cactattaat agcatcaggc aaaaaccttg cataattata
      901 agtaggtata ataacggtaa tcatttgcgc aacgtactat agagttgata aggaagccag
      961 tagtaaagtg gcatgttatt acgctgcgcc tgatggcgtt gtttaaataa ggtttgttgc
     1021 atttgtggtt tgccgctaat acccgtaaca tcaaatacag ccactggcat atttatatac
     1081 cgggtggttg cattatgctt ataaatcaaa cgtagcagta gttcataatc ggctgcaatt
     1141 gtgtaatcaa tattgtaacc attacatgct tttagtaatt cggttcgata aaaagtagca
     1201 gggtgccaaa ttgtatcgcg aagcatatgc ctagcagtta agtatgccgg ttgtttccgc
     1261 tcggtagcta ttttatcact aaaaaacaaa ttgccataaa tcaagtcttc tgttgcctta
     1321 agcctaacta cgttgcgtac tacgtcatca ctacaaaata tatctccgga gttaagaaat
     1381 ataatgtacg taccggtagc tttatgcaaa cctttattca tggcatcata aattccattg
     1441 tcgggctctg aaataacatg attaatttta gcccgataag gcattattaa ttccaaacta
     1501 ttgtcagccg atgcgccatc aattattata tattcaattg ctgcatagtt ttgactaaaa
     1561 acacttttaa cggtttgaaa taaaccaccg gcattgtttc ggtttatggt aacaatactt
     1621 attagcataa taccgattga taaagtgtaa tatgcatttg taatactgta gtcgaactta
     1681 attttttatc ggcatcggat gcaatctgtt ttcggttaaa atgctgatta ttagcaatac
     1741 aattatcaat tgccattaat agctgtgttg cattaaaatc aggtattaaa acgccattat
     1801 tattatcaat cagttcagga ataccaccgg tattaaaagc cacaacgggt gtgccacagc
     1861 ataaggattc gcagattgta tttggaaaat tttcggccaa tgatggtagt acaaatacat
     1921 tggcagctac ataccaccat tgcaattctt ccggtgttaa atagcccaat tgatttatgc
     1981 aagagggcaa attggcatcc ggcttaccaa ttccataaaa tttatgtttg cttttgttag
     2041 ctgctacaat aataaactgt atgcctttgc gcatattatt tacatcgttt gctacaaaca
     2101 acactacggt ttcgtttggt ggcagattaa gcttgttacg actgtcaatt aatggcttgt
     2161 atgaaaactg ttgt
//
LOCUS       DAOMAA010000332         2172 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_48356_length_2172_cov_2.850732, whole genome shotgun sequence.
ACCESSION   DAOMAA010000332 DAOMAA010000000
VERSION     DAOMAA010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48356_length_2172_cov_2.850732"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2172)
                     /locus_tag="PLO59_05270"
     CDS             complement(<1..>2172)
                     /locus_tag="PLO59_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00550.1"
                     /translation="MLSAPFKQFDNEAKMLNTIRNAGKESWLTEYNHYDRSRCTHDTW
                     AHGLFVASQTLCFFEDTNITKVLCHALTGDGRLGALFNDSSALRDYEKYRGDVDCSLN
                     APYTIPHEKSAAGTALAQVAHAIQNSTNKRKLLFGNGSRTFTTGHSTLYGWQFDNNNF
                     TEAIIMNLDSIRQDITFDTAQWNLNGSLYQQIFPGPGGPLDFIIGNAKYKPDSNELKI
                     ILDRQSSQRLSLPAYSLTRIWVSKNKIYLKAADTLLCNGTFTQIQAYGGKKYTWSGAK
                     FDYLTPDSSLVKFTNGNNKNQVYTVIATDQNGVTASINITVTETPSLTVSTNKNVVCA
                     GNEAKLTATLSTSNPNAVFHYVWVPSQTIVNPNLSICKVYPTKTTTYTVYATDGKCFA
                     PYDSVTIKVAPYAEAPKDTIITLGSSIMLKPLSTQTNVTFNWYEGSNFLGTDSALITP
                     NQTTVYTLIGNNLGSCTDTDYVTVQVVEVCNSATDTALTFKPYTTIKEFALKMIDYCT
                     VTGACIATDTLLKNFTGEIVFNGPLILNDNFSFSNCTNLKFGEGAYIEHNKDNRLLEF
                     INCQLSAAACNNKMWDGIKMLEPGEKLTLRNTTIADATVGINSFNNAVVVLDSCTFLN
                     CYIGVDFERYDESEAEGKVSATKFLGNTLTLEPYAGKTSFAGILCDNASVLNFGDSKE
                     QPNIFSGSLFGIYSINTSLELHNNIFENLVTDTTLGQPYTG"
BASE COUNT          631 a          390 c          412 g          739 t
ORIGIN      
        1 taccagtata gggttggcct aaagttgtat cggtaaccaa attttcgaaa atattgttgt
       61 ggagctcaag cgatgtgttt atggaatata taccgaatag tgagcctgaa aaaatattag
      121 gttgctcttt tgaatcgcca aaattaagta cagaagcatt gtcgcataaa atacctgcaa
      181 atgatgtttt gcctgcatag ggttctaatg taagtgtatt acctaaaaat ttggtagcac
      241 ttacttttcc ttcggcttcc gattcatcat atctttcaaa atcaacacct atgtaacaat
      301 ttaaaaatgt acaactatct aatactacta ctgcattgtt aaagctgtta attccaacag
      361 ttgcgtcagc aatggttgta tttcttagcg ttagcttttc gcccggttcc aacattttta
      421 tgccgtccca cattttgtta ttacaagccg ctgctgataa ttggcaattt ataaattcaa
      481 gcaggcggtt atctttgtta tgttcaatgt atgcaccttc gccaaactta aggttggtac
      541 aatttgaaaa actaaaattg tcgtttaaaa tcaatgggcc attaaacaca atttcgccag
      601 taaagttttt taataaagta tcggttgcaa tgcatgcacc tgtaacggta caatagtcaa
      661 tcatttttaa tgcaaattcc tttattgtag tataaggttt aaacgttaat gcggtatcag
      721 ttgccgagtt gcatacttca acaacctgca ctgttacata atcggtatcc gtacaacttc
      781 ctaaattatt tcctattaat gtgtaaacag ttgtttggtt gggtgttatc aaggctgagt
      841 cagttcctaa aaaattactg ccttcgtacc aattaaatgt aacattggtt tgtgtactta
      901 atggtttaag cataatgctg ctacctaatg taatgatggt atcttttggt gcctcagcat
      961 aaggtgcaac tttaattgta acagagtcat aaggtgcaaa acatttcccg tctgttgcat
     1021 aaactgtata tgtagtcgtt ttagtaggat ataccttaca aattgacaag tttgggttaa
     1081 cgattgtttg tgatggtacc caaacgtaat gaaatactgc attgggatta ctggtactta
     1141 aagttgcagt aagtttggct tcgttacctg cacataccac atttttattg gtactaaccg
     1201 taagcgatgg tgtttcggta acggtaatat ttatggaagc tgtaacacca ttttggtcgg
     1261 ttgcaattac tgtgtaaact tgatttttat tattaccatt tgtaaatttt accaagctgc
     1321 tgtcgggggt taagtagtca aacttagcac cactccaggt gtattttttt ccaccatagg
     1381 cttgaatttg ggtaaaggtt ccgttacaca ataacgtatc ggcagctttt aaataaattt
     1441 tgtttttcga aacccaaatt ctggttaatg aataagccgg taagcttagt ctttgactcg
     1501 attgtctatc taaaataatt ttaagttcat tactatcagg cttgtatttg gcattgccaa
     1561 ttataaagtc cagagggcca cctgggccag gaaaaatttg ttgatacaaa ctaccattta
     1621 ggttccattg cgcagtatca aatgtgatgt cttgtctgat actatccagg ttcataatta
     1681 tagcttcggt aaaattatta ttgtcgaatt gccaaccgta caaagtgcta tgtccggtgg
     1741 taaaagtgcg actgccatta ccaaacaata actttcgctt atttgtagag ttttgaatgg
     1801 catgtgctac ttgtgctaaa gcagttccgg cagcgctttt ttcgtgtggt atggtataag
     1861 gcgcatttaa cgaacaatca acatcacctc tgtatttttc atagtctctt aaggcagatg
     1921 aatcattaaa aagtgcaccc aatcgcccat ctcctgttaa cgcatggcaa agcaccttgg
     1981 taatgttagt gtcttcgaaa aagcaaagtg tttgcgaagc aacaaataaa ccatgagccc
     2041 aggtatcatg tgtacatcgg cttctgtcat agtgattgta ttcggttaac catgattctt
     2101 tacctgcatt tctgatggta ttaagcattt tagcctcatt gtcaaattgc ttgaaagggg
     2161 cgctaagcat ca
//
LOCUS       DAOMAA010000333         2169 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_48499_length_2169_cov_2.768685, whole genome shotgun sequence.
ACCESSION   DAOMAA010000333 DAOMAA010000000
VERSION     DAOMAA010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48499_length_2169_cov_2.768685"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..869)
                     /locus_tag="PLO59_05275"
     CDS             complement(27..869)
                     /locus_tag="PLO59_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00551.1"
                     /translation="MKNYLNTSMMLLITLAVIISGCRKNDTDELESATEVSRDNAASQ
                     NVTDDVLQQVDDAAMRSADIDPLTSLSPSGTCPTISIITTNGSWPKTVIIDYGNTNCS
                     ANGNTLRRGKIIANFTGPYRQVATIVTIGFDNYFVNNNKVTGTKTITNLGNNSSGQMQ
                     WTVNVQQAKITRPDSSFITWDATRLRTWVTGQNTLNNRNDDAFEIMGTTTGSNSNGHS
                     FISTVTVPLYVAWGCDYIQAGTLDFTPSGKPTRTINFGTAGVCDNQATLTVNGNTYNI
                     TLKP"
     gene            complement(981..>2169)
                     /locus_tag="PLO59_05280"
     CDS             complement(981..>2169)
                     /locus_tag="PLO59_05280"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HQV00552.1"
                     /translation="IILSISLVNIIAVIIKALKQGKDGARIFALVILLAPVMSYMASK
                     LKTVALFDTLLTDINSGAVFTISLIISLPLSMTIYLARDMARMGKRLTKQLDEITNLS
                     NKTIEQEQEKKRILENQNLDLERNVQQRTLEVVQQKAELEIKNKEITESLHYAKRIQA
                     AILPPTELFTTTFADAFILYLPKDIVSGDFYAYAKKENNIIIAAADCTGHGVSGALMS
                     MIGSSLLNQIVNEKNIIEPGHILNTLNEGIIDALKQRESTSHDGMDIAICQLNLQTLT
                     LRFAGANRPLWLLRNHEIISYQPNKFPIGGLQILHDEVYAEHTIALQKNDVLYLFSDG
                     YADQFGGLHEKKLLTKNLKNHILEIHHLPLSQQRESLLQTFNSWKGKLEQVDDVLVLA
                     VKI"
BASE COUNT          593 a          402 c          442 g          732 t
ORIGIN      
        1 gacttaagcc aaacggcatt ttataattaa ggtttcagtg taatgttata cgtattgcca
       61 tttacggtta atgttgcctg gttatcgcaa acaccggctg tgccaaagtt tatggtacgt
      121 gtaggtttgc ccgatggtgt aaaatctaac gtgcctgctt gtatatagtc gcaaccccaa
      181 gccacataca atggcacggt tacagtcgaa ataaaactat ggccattgct gttgctgccg
      241 gtagtggtac ccataatttc aaaggcatca tcgttacggt tgtttaaggt gttttgacct
      301 gtaacccagg tgcgcaaacg tgtggcatcc caggttataa agctactgtc gggtcgggta
      361 attttggctt gctgtacatt aacagtccat tgcatttgcc cgctactgtt attacctaaa
      421 ttggtaatgg ttttagtgcc ggttactttg ttattgttta caaaataatt gtcgaaacca
      481 atggtaacaa tggtggccac ctgccgatat gggcctgtaa agtttgcaat tattttaccc
      541 ctgcgtagtg tattgccatt agcactgcaa ttggtgtttc catagtcaat aattacggtc
      601 tttggccaac tcccgttagt ggtaataatg ctgatagtgg ggcaagtacc cgatgggctt
      661 aacgaagtta acggatcaat atcagcgcta cgcatagctg catcgtcaac ctgttgtaat
      721 acatcatcgg ttacgttttg tgatgcagca ttatcgcgcg atacttctgt tgccgattcc
      781 aattcgtctg tatcattttt acggcaacct gaaataataa cggcaagtgt tatcaatagc
      841 atcatgctgg tattcaagta atttttcata gcttaataag tttatatgtt taagttaaag
      901 caaggcgcta cttgctgttt gcacatgcat ggttaggatg caaatacaaa acgaaggttt
      961 aatgcgataa aaaattattg tcagatttta acagctaaaa ccaacacatc atctacttgc
     1021 tccagtttgc ctttccaact attaaatgtt tgaagtaaac tttcacgttg ttgtgatagt
     1081 ggcaggtgat gtatttccaa gatatgattt tttaaatttt ttgtgagcaa ttttttttca
     1141 tgcaagccac caaactgatc ggcatagcca tcagaaaaaa gatataacac atcattcttt
     1201 tgtaatgcaa tggtatgttc ggcataaacc tcgtcatgca aaatttgcaa acctccgata
     1261 ggaaatttat taggttggta acttattatt tcgtggttac gcaaaagcca taatggcctg
     1321 ttagcaccgg caaaacgcag ggtaagcgtt tgtaaattaa gttggcatat agctatatcc
     1381 ataccatcgt gcgatgtgct ttcgcgttgc tttaaggcat caataattcc ttcgttaagt
     1441 gtatttaata tatgtcccgg ttcaattata tttttttcgt ttactatctg attaagtagc
     1501 gaactgccaa tcatactcat aagtgcacct gatacaccat ggccggtaca atcggctgcg
     1561 gcaattataa tgttgttttc tttttttgca taggcataaa aatcgccact tacaatgtct
     1621 ttaggcaggt ataatataaa tgcatcggca aaggtggttg taaacaactc agtaggtggc
     1681 agtatggctg cttgtatgcg ttttgcataa tgcaaacttt cagtaatctc tttatttttt
     1741 atttcgagct cggctttttg ttgcactact tctaaggtgc gttgttgcac attacgctct
     1801 aagtcgaggt tttgattttc gagtatgcgt tttttttctt gttcttgttc aatggttttg
     1861 tttgacaggt tggtaatttc atctaactgt ttggttaacc gcttacccat gcgggccata
     1921 tcgcgtgcca ggtaaatagt catactaagt ggcagcgata ttatgagtga aatagtaaaa
     1981 acggctcctg agtttatgtc ggtaagtaat gtatcaaata aggctactgt ttttaattta
     2041 gaagccatgt aagacattac cggtgccaat aaaattacca gtgcaaaaat gcgggcccca
     2101 tctttacctt gctttagggc ttttattatt acagcgataa tatttactaa cgagatactt
     2161 aaaattatt
//
LOCUS       DAOMAA010000334         2167 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_48584_length_2167_cov_2.478693, whole genome shotgun sequence.
ACCESSION   DAOMAA010000334 DAOMAA010000000
VERSION     DAOMAA010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48584_length_2167_cov_2.478693"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="PLO59_05285"
     CDS             complement(<1..522)
                     /locus_tag="PLO59_05285"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HQV00553.1"
                     /translation="MNLLSRIFVSIVIACALVFNSTNAQISLPSDFADEVILTNVTEV
                     TGVAIDSSGKIFVSQHDGTVWRVNNGVKVSPPIIDISQECGRWNAHGLMSITLDKDFT
                     TNGYIYLYYTVDRHYLLNYGTPNYNPNANEKNKATIDRVTRYQIDPTTNFTTLVPNSR
                     FILLGKDKKNGISI"
     gene            complement(563..>2167)
                     /locus_tag="PLO59_05290"
     CDS             complement(563..>2167)
                     /locus_tag="PLO59_05290"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV00554.1"
                     /translation="TQIFIGDIGNNSGNRTNLRIYIVNKVDFQTNVNSVMAQIINYSY
                     SDQTNFTPATNNTNFDAEAFIYLNDSLHIFSKNWIDKKSKHYILPAKAGNYQAQLLET
                     LNAGYLVTGASVQKAGVIVLCGYDNTGIAPVYITLLFDYNNALFFNGNKRRFNVSNAL
                     THGQVEAIDFTGTGFGWISNERFQQSVFNVAPKLKSFNISSFLPAKFLVPNPVAGIQS
                     NSQIICKNKTVTFTSNSSNATQLQWYFDGGLPAGSTDSVVEVQYKTTGLFDVTLIASN
                     GFTTDTLILSNYITVNQTPKVSITALGSTVFCTGGVMLLANANNAVSYQWRKNGKPIT
                     GAITTNYLATESGSYSVTAYNTNGCARLSNVIVVNGPPVATASITGSLNICPPDSVII
                     TATAGNMSYQWLRNGNVMTGANQQSLTVTVAGRYNVNITNSSGCMVTTPKKKVTISCR
                     INPQELQNLIFPNPSNTHFEINSGMLKSKCLTINVFDIWGREIQSYNSENTNAWPIFG
                     GSLKKGIYVVQVIAETATYYCRIAKN"
BASE COUNT          623 a          397 c          416 g          731 t
ORIGIN      
        1 tatggatatg ccattctttt tgtccttgcc caataatata aaccggctgt tgggcactaa
       61 ggttgtaaaa ttggttgttg ggtcaatctg ataacgggta accctgtcga tggtggcttt
      121 gtttttttcg ttggcattgg ggttataatt gggtgtaccg tagtttaaca ggtagtgtct
      181 gtcaaccgta taatacaggt aaatataacc gttggtggta aaatctttat ccaatgttat
      241 actcattaaa ccgtgtgcat tccaccggcc acactcctga ctaatatcaa taataggcgg
      301 gcttacttta acaccattat ttacacgcca aaccgtgcca tcgtgctggc ttacaaaaat
      361 tttaccgctg ctatcaatag caacaccggt tacctcggta acattggtta gaattacttc
      421 atcagcaaaa tcggatggta aagaaatttg tgcatttgtt gaattaaata ccaatgcaca
      481 tgcaattaca atagaaacaa aaattcgtga taacagattc attataaata gattttaaag
      541 tctttttatt tatggggcaa aatcaatttt tggcaattcg gcaatagtat gttgcagttt
      601 cagcaatgac ttgcactacg taaataccct ttttcaaaga gccgccaaat ataggccaag
      661 cgttggtatt ttccgaatta taagattgga tttctcgacc ccaaatatca aatacgttta
      721 tagttaaaca tttagatttt aacatccctg aatttatttc aaaatgggta ttgcttgggt
      781 taggaaatat aagattttgc aattcttgcg gattgatgcg gcaggaaata gttacttttt
      841 ttttgggtgt tgtaaccata caacctgagc tattggtaat gtttacgtta tatcggccgg
      901 ctactgttac agttaaactt tgttgatttg caccggtcat tacgtttccg ttgcgcaacc
      961 attgataact catattacct gctgttgcgg ttattattac actgtcgggc gggcaaatat
     1021 ttaaactgcc tgtaatagaa gctgtagcta ccggggggcc atttacaact attacattgc
     1081 ttaaccgtgc gcatccgttt gtattgtagg ctgttacgct gtagctgcca ctttctgtag
     1141 ccaaatagtt tgtggtaatt gcaccggtaa tgggtttgcc attcttacgc cattgatatg
     1201 aaaccgcatt gttagcatta gctaacagca taacgcctcc tgtacaaaaa acagtactgc
     1261 ctaaggctgt tatactaact ttaggcgttt gattaactgt tatgtaattt gataaaatta
     1321 aagtgtctgt ggtaaatcca ttgcttgcaa ttaaggttac atcaaacaat ccggttgttt
     1381 tgtattgaac ttccactaca ctatcggttg aaccggctgg caaaccacca tcaaaatacc
     1441 attgtaattg tgtagcatta ctgctgtttg aagtaaatgt tacggttttg tttttgcata
     1501 taatttgact gttgctttga atgcctgcaa cgggattggg cactaaaaat ttagcaggta
     1561 aaaagctact tatattaaat gactttaatt ttggagccac attaaaaacc gattgttgaa
     1621 accgttcatt gctaatccat ccaaagcctg taccggtaaa gtcaatggct tctacttgcc
     1681 catgtgtaag tgcattcgaa acattaaacc gtcttttgtt tccattaaaa aacaaggcat
     1741 tgttgtaatc gaaaagcagt gtaatataaa caggagcaat gcccgtgttg tcataaccac
     1801 aaagcacaat tacaccggct ttttgtacac tggctcctgt aaccaaatag ccagcattta
     1861 gcgtttcgag taactgcgct tggtaattgc cagcctttgc cggcagtatg taatgtttac
     1921 tttttttatc tatccagttt tttgaaaata tatgtaatga atcgtttaaa taaataaaag
     1981 cctcggcatc aaaattcgta ttgtttgttg ccggggtaaa gttggtttgg tcgctatagc
     2041 tgtaatttat tatttgcgcc ataactgaat tcacattggt ttgaaagtcg actttgttta
     2101 caatgtaaat acgcagattg gttctatttc cgctgttatt gccaatatca ccaataaaaa
     2161 tttgtgt
//
LOCUS       DAOMAA010000335         2165 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_48664_length_2165_cov_2.935545, whole genome shotgun sequence.
ACCESSION   DAOMAA010000335 DAOMAA010000000
VERSION     DAOMAA010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48664_length_2165_cov_2.935545"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..489)
                     /locus_tag="PLO59_05295"
     CDS             complement(7..489)
                     /locus_tag="PLO59_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00555.1"
                     /translation="MENYGIKSWAEEDRPREKLITKGSRALSDTELLAIFIRTGTQKK
                     TAIDVSRELLQAAQNNLNILGKMSVQQMIKLNINGLGVVKATTIVAALELGRRRREVA
                     ENITHKKIITSKDAYHALITDLEFLQVEEFWILLLDRSNQIIKKIKKTCKGIDSLMTK
                     "
     gene            complement(610..864)
                     /gene="rpsT"
                     /locus_tag="PLO59_05300"
     CDS             complement(610..864)
                     /gene="rpsT"
                     /locus_tag="PLO59_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HQV00556.1"
                     /translation="MANHKSSVKRIRSTETKRARNHYQAKTMRNALKALRSNKSKIEV
                     AKLIPSVSAMLDKLAKTNVIHKNKAANLKSSIAKHYNAMA"
     gene            complement(928..1002)
                     /locus_tag="PLO59_05305"
     tRNA            complement(928..1002)
                     /locus_tag="PLO59_05305"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(967..969),aa:Glu,seq:ttc)
     gene            complement(1142..1822)
                     /locus_tag="PLO59_05310"
     CDS             complement(1142..1822)
                     /locus_tag="PLO59_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008610726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQV00557.1"
                     /translation="MSNADFKILLVDDEPDIIEFIEYNLKKEGFQIYTAHNGKEGIQQ
                     AIKHQPHLIVLDIMMPVMDGIETCRQLREMKDFKNTVIAFLTARNEDYSQIAGFDVGA
                     DDYINKPIKPRILISRIKALLRRTSDETTDEPIKIGDIEINRDKYSVSKNGVVLNFPR
                     KEFELLSLLVSRPGKVFTREDILNRVWGEDIVVGDRTIDVHIRKIREKLGDENIKTIK
                     GIGYKFDI"
BASE COUNT          609 a          401 c          365 g          790 t
ORIGIN      
        1 acttcctcat ttagtcataa ggctgtctat tcctttgcat gttttcttta ttttttttat
       61 aatctgattg cttctgtcga gcagtaatat ccaaaactct tcaacttgca aaaactccag
      121 gtctgttatc agtgcatgat aggcatcttt actggtaatt atttttttat gggtaatatt
      181 ttcggctact tctctgcgtc tgcgccccaa ttccaatgca gctactatcg tggtggcttt
      241 taccactcct aagccgttaa tgtttagctt tatcatttgc tgcacactca ttttacccaa
      301 aatatttaaa ttgttttgag cggcttgtaa taattcgcgg cttacatcaa tggccgtttt
      361 tttttgtgtt ccggtacgga taaaaatggc taacaattca gtgtcggaca aggccctgct
      421 tccttttgta attaattttt cgcgtggcct gtcttcttcg gcccatgatt ttataccgta
      481 attttccatt ggcaagttat ttagtgtaag atgcatcaat attaaaacat accaccaggt
      541 ttagcacaaa aaaaaacacc tccggatttt atttccgaag gtgttttttt atattttttt
      601 taatgttgct tatgccatag cattataatg tttggcaatg ctcgatttta aattggctgc
      661 tttgtttttg tgtattacat tggttttggc aagtttatca agcatggccg ataccgatgg
      721 aataagttta gccacttcga ttttactttt gttagagcgt agcgctttta aagcattacg
      781 catagtttta gcttgataat ggttgcgcgc acgcttggtt tctgttgaac gaattctttt
      841 tacagatgat ttgtgatttg ccattgcaat gtatctttta attgtttgtg tttagtttga
      901 aatctaaacg aattctcaaa ataaaaatgg tagtccgtac gggaatcgaa cccgtgtttt
      961 atccgtgaaa gggatacgtc ctaaccccta gacgaacgga ccatttttaa aaatgaggct
     1021 gcaaaagtat tatttttttt aatcgtacaa acttttcaaa acaaaaaaga agccttccgt
     1081 tatttagcag caaaaatgcc gcaaactctt atattatagg ggtttcgaag gatgttgggt
     1141 tttaaatgtc aaatttatat ccaataccct tgatggtttt aatgttttcg tcacccagtt
     1201 tttcgcgtat ttttcgaatg tgaacatcaa tcgttctatc gcccacaact atatcctcgc
     1261 cccaaacccg gtttaaaata tcctcacgtg taaatacttt gcccggacgc gacacaagca
     1321 aactcaacaa ctcaaattct ttgcgtggaa aatttaaaac cactccattt ttacttacgc
     1381 tgtatttatc acggtttatt tcaatatcgc ctattttaat aggctcatct gtagtttcgt
     1441 ctgaggttct gcgcagcagg gctttaatgc ggctgataag tatgcgtggc ttaataggtt
     1501 tgtttatata atcatcggca cctacatcaa agccggcaat ttggctgtaa tcttcgtttc
     1561 gtgcggttaa aaaggctatt acagtatttt tgaaatcctt catttcgcgt aattgacggc
     1621 atgtttcaat gccatccatt acgggcatca ttatatctaa tacaattaga tgtggttgat
     1681 gctttatggc ttgttgtatg ccttctttgc cattatgagc cgtataaatt tgaaaacctt
     1741 ccttttttaa attgtattca ataaactcaa taatatccgg ttcatcatca accaataaaa
     1801 ttttaaaatc tgcgttcgac atggcaatct aattttgttg caaacaaaca agcggcaaat
     1861 aaccgaatag ttacctgcgt tttaccaaat tgttaaataa caatttgtaa aggtaaacat
     1921 aactttagcc tcaatggctg cacaattgat taacaaatct tataatccat aacattaact
     1981 taatatacta ataaccatta agtgatttag taggttgttt tttgcggcta tgaaagcgaa
     2041 aattattgtt ggttaatcat tgattaacgg tccgataata tttgcgaaac ttttaatacg
     2101 ctttaatttt taattatgac aaatagcatc atcaaaattg gtattataac catagcagta
     2161 gcatt
//
LOCUS       DAOMAA010000336         2161 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_48837_length_2161_cov_3.235518, whole genome shotgun sequence.
ACCESSION   DAOMAA010000336 DAOMAA010000000
VERSION     DAOMAA010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48837_length_2161_cov_3.235518"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(254..1873)
                     /locus_tag="PLO59_05315"
     CDS             complement(254..1873)
                     /locus_tag="PLO59_05315"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV00558.1"
                     /translation="MKTKNKLYVAVVFVLISCVNTGLLYAQSVCMHNFKENRNTIQAK
                     AFKSVVLGNDDLVSTGFRVVNGVNSIVADRYDAATCSLASTFIFPYYSGPPATAEMQV
                     IVSNDNKVGIRYFDTNWPGYNYFFVLDSMLNQISSLVLGNSVTNRLIAFANDGDVILT
                     QLSSAPSFGETLIVNKLDQANLVLSDWSTAIYFPGSQIRMVSDIKVDSSNNTYLTGCT
                     EKSGVGLRLFVLKLNAAGQKEWVNQYIHPNLDWNDWLFTKLVVNDEQNVITASRRINN
                     NANKLNLYCHAADGTLLWSKQENIDTLSNKVWLGTDGLNNVYTATHLIGVQNYPSTIV
                     FKYDKNGNKLWQRTLSNFRASSMKVANNGFLYLAGDHYFTQTLTPGYRSRFLQLDANG
                     NTIGKENQIHNYNTSTVDSSYSKYNDVVIKSNGNAYLVGERRDYKPIGTTDYYVVDSY
                     EKNMLPILKQQLPASDFTVSIMPNPASNFISIETALTNYRFTIFDLSGLVKMQGHPLN
                     QTINIETLTPGMYFVRLACNEHISILKIVKQ"
BASE COUNT          687 a          374 c          368 g          732 t
ORIGIN      
        1 ttgaagctcc tgaagatggc gaacctaccg gtgatgaaaa actttaaacg gttttattca
       61 gataatcagc atgcacattt aaatgcatct tgccgtattt gatttgcttc gaaacaccct
      121 agttgttagt gtttcctatt tacattgata aattaaaaaa cagccggctc tttcgaaacc
      181 ggctgtgtgt tgccaaacaa aatttacaat gtgtagtata taggtatagt tataaaagcg
      241 tattggatat tgcttactgc tttactattt tcagtatgct tatatgttca ttacatgcta
      301 accttacaaa gtacataccc ggagttagtg tttcaatatt tatagtttgg tttaatggat
      361 ggccttgcat ttttacaagg ccacttaaat caaaaattgt aaagcggtag tttgttagtg
      421 ctgtttctat tgatataaaa ttacttgccg ggttaggcat tatgcttact gtaaaatcgg
      481 atgcaggtag ttgctgtttt aatattggaa gcatattttt ttcataacta tcaaccacat
      541 aataatcggt ggttcctata ggcttataat cgcgtctttc gcctactaaa taagcattgc
      601 cattactttt tattacaaca tcattatatt tggagtagga tgaatcaacg gttgaagtat
      661 tgtaattatg gatttgattt tcctttccga ttgtattgcc gttagcatct aattgtaaaa
      721 aacgcgaacg gtaaccgggc gtaagtgttt gcgtaaagta atgatcgcct gctaaatata
      781 aaaatccatt gttagccact ttcatagaag aagctctgaa atttgacaat gttcgttgcc
      841 ataatttatt cccattttta tcatatttaa atacaatggt tgatggataa ttttgtacac
      901 ctattaaatg tgtggctgta tatacattgt ttaaaccatc ggtacctaac caaactttat
      961 tcgaaagcgt atcaatattt tcttgcttac tccataacaa agttccgtct gcagcatggc
     1021 agtagaggtt tagtttattt gcattgttat taatgcgtcg cgatgcggta ataacatttt
     1081 gttcatcatt aacaacaagt ttggtaaaaa gccaatcgtt ccaatctaag tttgggtgta
     1141 tatattggtt tacccattct ttttggccgg ctgcatttaa ttttaataca aacaaacgaa
     1201 gtccaacacc actcttttca gtacaaccgg taaggtaggt attgttgctg ctatcaacct
     1261 taatgtcgga caccatcctt atttgagaac ccggaaaata tattgctgtt gaccagtcgg
     1321 acaaaactaa attggcttgg tctaatttgt ttacaattaa cgtttcgcca aacgaaggcg
     1381 cactgcttaa ttgggtaaga attacatcgc catcgtttgc aaatgcaatt aagcggttgg
     1441 taacactatt gcccaaaacc aaactactta tttgattaag cattgagtcg agcacaaaaa
     1501 aataattata cccgggccag tttgtgtcaa aatagcgtat gcctacttta ttatcattac
     1561 taactattac ctgcatttcg gctgtggcag gtggccccga ataatatgga aatataaagg
     1621 ttgacgctaa tgaacatgta gcggcatcat aacgatcggc tactatgcta ttaacgccat
     1681 ttacaacccg aaaaccggtt gataccaagt catcatttcc aagtacaacc gatttaaatg
     1741 ctttagcctg aatggtattt ctgttctctt taaagttatg catgcaaacc gattgtgcat
     1801 aaagcaagcc tgtgtttaca caacttataa gtacaaatac aacagccaca taaagtttgt
     1861 tttttgtttt catggcaaag ttttgtgtta agtaaaaaag tatccgaaat caaattccaa
     1921 cagaaagaaa gataaacact atttgtcaaa actatctgta aagtaactta taaagttatt
     1981 ggctgcttaa actactaaaa ataacttatc tgataaaatt gctgcaactt aaattcacaa
     2041 gtaaaattct atagcagcta caatttttat caaatataaa attaaaagga tgtgttctga
     2101 tattaaattt atgaggtaac aaattatagt tttgaatatt tatttcgcat tgcaactcac
     2161 a
//
LOCUS       DAOMAA010000337         2159 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_48943_length_2159_cov_2.585551, whole genome shotgun sequence.
ACCESSION   DAOMAA010000337 DAOMAA010000000
VERSION     DAOMAA010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48943_length_2159_cov_2.585551"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2159)
                     /locus_tag="PLO59_05320"
     CDS             complement(<1..>2159)
                     /locus_tag="PLO59_05320"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HQV00559.1"
                     /translation="NTSNNNIYVINTDSVLIEVIPQDGMYHILRDTLLLPSYGMTELV
                     DNGEDSLRLITGKFPIAHLRKLDSIPNLVNYARPYYPPVNSRATAVVLPGIAYSQGDA
                     AMRSDTARMIFGVDGSNVKVGVISNSYNTQANNKAGDDVIAGDLPSNVELLLDYPYGR
                     QTDEGRAMMQIVHDVAPGAQLAFRTGYISAGDFAEGIKELQQANCDVIVDDISYITEP
                     FFQDGVISHAVNAVRDSGVVYIAAAGNYGSRSYGSTFNPVTAPSGFAGHAHDFGGGDV
                     YQRVNLPHGTYTIVLQWEDSIYSRGQLPGVANDLDIYLLDTTGNIIFGFNRNNIYGDP
                     LEVMPFIVKGNITADLLITRAQGNQNVRFKYIVFRGEMDITEHNNESGTVVGQSNAQG
                     AITVGAVLYTNTPAYGVSQPTVASFSSRGGNILSGESTARFKPNICAPNGVNTTVKMG
                     GVNIDGDALPNFFGTSAAAPHIAGVAALVIDAQNRFNQNTISPDSVRDLISQTALDMY
                     GSGYDINSGYGFVRTDSLLARFSSPKPVLYSLAVDTTVQSITGSTQPEFKGAYFNEHT
                     KIIMRDDTLPTQVIDANTIIAEAPAFEGNPPFFAYNPPTTPSGLDGGNSDSLYFFSPV
                     KKQIVVRPNDVVKYYGEDLPLLSFSVTVDGQPLDSTQLTLNDLNLTNVLLSTSVQAFT
                     NPGLYPVTATNSPLSASPVSQVVLDELYKFTYQPGVF"
BASE COUNT          631 a          451 c          433 g          644 t
ORIGIN      
        1 aaacaccccc ggttgatatg taaatttata caactcgtct aataccacct ggctaacagg
       61 cgatgcagac aaaggactat ttgtagctgt aactgggtat aagcccgggt tggtaaacgc
      121 ttgtacagat gtacttaata gtacattggt taaatttaaa tcatttaatg taagttgtgt
      181 cgaatctaat ggttggccat caaccgttac actaaaactt aacaacggta aatcttcgcc
      241 ataatactta accacatcat ttgggcgtac aacaatttgt tttttaaccg gactaaagaa
      301 gtacaaacta tcactattac caccgtctaa acctgatggg gtagtaggcg gattgtatgc
      361 aaaaaatggc ggattgcctt cgaatgcagg tgcttcggca ataatggtat ttgcatctat
      421 aacttgtgta ggtaatgtgt catcgcgcat aataattttg gtatgttcat taaaataagc
      481 gcctttaaat tcaggttgtg tactgcctgt aatactttgt acagttgtat caaccgctaa
      541 actatataat acaggtttgg ggcttgaaaa gcgtgctaac aatgaatcgg ttcttacaaa
      601 tccataaccg ctattaatgt catatccact gccatacata tcaagagccg tttgcgaaat
      661 taaatctctt acactatcag ggctgatggt gttttgatta aaacgatttt gagcgtctat
      721 tacaagtgct gcaacacctg ctatatgtgg tgctgctgcc gatgtgccaa aaaagtttgg
      781 taaggcatcg ccatcaatat ttacaccgcc catttttact gttgtgttta caccattagg
      841 cgcacatata tttggtttaa aacgtgcagt gctttcgcca cttaaaatat taccacctcg
      901 agatgagaag gaggctaccg ttggttgact aacaccataa gcaggtgtat ttgtatataa
      961 cacggctcca acagttatag cgccttgtgc atttgattga ccaactacag tgcccgattc
     1021 gttgttgtgc tcggtaatat ccatttcgcc tctaaataca atgtatttaa atcgtacatt
     1081 ttggttgcct tgcgcacgtg taattaatag atcggcagtt atatttcctt ttacaataaa
     1141 tggcattact tcgagcggat cgccataaat attgttgcgg ttaaacccga aaataatgtt
     1201 gccggtagtg tctagtaaat agatgtctaa atcattggca acgcccggca attgcccacg
     1261 cgagtaaata gagtcttccc attgcaaaac aatggtgtat gtgccatgag gcaaatttac
     1321 acgttggtaa acatcgccac caccaaagtc atgggcatgg cctgcaaaac ctgatggtgc
     1381 agttacaggg ttaaatgtag aaccataaga acggcttcca taattaccgg cagcagcaat
     1441 atataccaca ccgctatcgc gtactgcatt tactgcatgg ctaattacac cgtcttgaaa
     1501 aaacggttcg gtgatatacg aaatgtcatc tacaattaca tcgcaattag cttgttgtag
     1561 ttctttgatg ccttcggcaa aatcaccggc acttatatat ccggttctga atgctaattg
     1621 ggcaccggga gcaacatcat gcacaatttg catcattgca cgaccctcgt cagtctgacg
     1681 gccataaggg tagtcgagca acaattctac attgctgggc aagtcgcctg ctatcacatc
     1741 atcgcctgct ttattatttg cttgtgtgtt ataactgttt gaaataacgc ctactttaac
     1801 attgctgcca tctacaccaa aaatcatacg tgccgtatcg ctgcgcatgg ctgcatcgcc
     1861 ctggctatag gcaataccag gtaacacaac tgctgttgca cggctattta caggcggata
     1921 ataagggcgg gcatagttta ccagattcgg aattgaatcg agtttacgta aatgtgcaat
     1981 tgggaattta ccggttataa gtcgcaacga atcttcgcca ttatctacca attcagtcat
     2041 accatagctt ggcaacaata aggtgtcgcg taaaatatgg tacataccat cttgcggtat
     2101 aacttcaata agtacactat cggtattaat tacataaatg ttgttgtttg atgtattaa
//
LOCUS       DAOMAA010000338         2156 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_49081_length_2156_cov_2.076630, whole genome shotgun sequence.
ACCESSION   DAOMAA010000338 DAOMAA010000000
VERSION     DAOMAA010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49081_length_2156_cov_2.076630"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(203..1006)
                     /locus_tag="PLO59_05325"
     CDS             complement(203..1006)
                     /locus_tag="PLO59_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00560.1"
                     /translation="MLRFYSLLGICFFLTHTKMYAQTSANFKVKKHSVYFAWGYNKDW
                     FGKSTIHFKSSSQSNFEYDFKWVKVSGKDYPGFNKIFSNNISIPQYVYRVGYINTKKG
                     WGIEYAFDHAKYIINHDKSIHMQGTVKGEYVDDYFMFDKDTMHLEHTNGANFAMINVL
                     REKSLYQSRSNKFDITYTLKAGAGIVVPKTDVTLFGERLDNRFHVAGYIVGAEAGCSF
                     LFYKSLYLMPSFKTAWANYTNVLTTDDARAKHHFMAYQLMLTMGYRYNF"
     gene            1266..1925
                     /locus_tag="PLO59_05330"
     CDS             1266..1925
                     /locus_tag="PLO59_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00561.1"
                     /translation="MRLFTFTTTGILSILPLCFLWAQKNPNPGKPVSTAAPKQTHQIE
                     MTSKSKNTMLLPSDKIIAERFADIQVLRYEVTGFESLTLKQKKLAYYLTQAGLSGRDI
                     YWDQKYKHNLKIRKLLEHILNTYSGSRVGEQWQLFESYCKQVFFANGIHHHYSNEKII
                     PAFKNEYFKELITNPTAGVAPKWLKVDSKSLKITVAAQPAREDITEELNEQAVVEFYS
                     R"
     gene            1951..>2156
                     /locus_tag="PLO59_05335"
     CDS             1951..>2156
                     /locus_tag="PLO59_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016997076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HQV00562.1"
                     /translation="MNKLIIPAIKSTKSTGDNSSKITISPLQGGYGRTLANSVRRVLL
                     SSIPGAGATGFRIEGVSNEFSTIDG"
BASE COUNT          762 a          388 c          364 g          642 t
ORIGIN      
        1 aggcagtaaa agaaaaatta gagtcactat tttttgctga tgctgcaaat acaattgcag
       61 gtaaagaatt tacttatgca tttagaataa aacctgtgtt ataatacaac cgtattttcg
      121 aagcatacgg gctgaggata ccgggatact aatttgcatg tagaagctgt ctgaaaaggg
      181 cagctttttt atttgcatta atttaaaagt tgtaacggta acccattgta agcattaact
      241 gataagccat aaaatgatgt ttggcacggg catcatcggt agttagtaca ttggtgtagt
      301 tagcccatgc ggttttaaaa gatggcatca ggtataaaga tttataaaat aaaaaactac
      361 aacctgcttc ggcaccaact atataacccg ctacatgaaa acgattatcc aaacgctcgc
      421 caaataatgt tacatcagtt ttcggcacta caatgccggc acctgctttc agtgtataag
      481 ttatatcaaa cttattgctg cggctttgat acaaactttt ttcacgcaaa acatttatca
      541 tggcaaaatt ggctccattg gtatgctcta agtgcatagt atctttatca aacataaaat
      601 agtcgtcaac atattcgcct tttacagtac cttgcatgtg aatggattta tcatgattga
      661 taatatattt tgcatggtca aaagcatatt caatacccca tccctttttt gtgtttatat
      721 aaccaactcg atatacgtat tgcgggatag agatattatt ggaaaatatt ttattaaatc
      781 ccggatagtc tttaccgctc actttaaccc acttaaaatc atactcgaaa ttgctttgac
      841 tactgctttt aaaatggatg gtgcttttac caaaccaatc tttattataa ccccatgcaa
      901 aataaactga atgttttttg actttaaaat tagccgaagt ttgtgcatac attttcgtat
      961 gcgttagaaa aaaacaaatt cctaaaagtg aataaaaacg taacataagt tggtttataa
     1021 aaaggttacg aaagtaaatg ccttatttaa aacagcgtgt taaataaggc ttaaacaaaa
     1081 aactgaacat acttattagc agcaaactta actaaaacca gattattcaa tttaagaaac
     1141 ggggataaag taacagatac ggtactgata aaattttgca gcagcaatca taaacaagcc
     1201 cccgccaatt atgttgtaaa tatttaattt gcttcattta cgcagtttac aataaatatt
     1261 aaatcatgcg actttttact ttcacaacta ccggtatttt atctatactt cctctgtgtt
     1321 ttttatgggc tcaaaaaaat cccaatccgg gcaaacctgt ttcaactgca gcacctaagc
     1381 aaacgcatca gatagaaatg acatcaaaat caaaaaatac tatgctattg ccatctgata
     1441 aaattattgc cgaacgtttt gctgatattc aagtgctgcg ttatgaagtt acaggctttg
     1501 aatcgcttac acttaagcaa aaaaaactag cctattatct tacacaagcc ggcctgtcgg
     1561 gacgagatat ttattgggat cagaaataca aacacaattt aaaaatcaga aaactgctcg
     1621 aacatatttt aaatacctat agcggaagtc gggttggcga acaatggcaa ctttttgaat
     1681 cttattgcaa acaagttttt tttgcaaacg gtattcacca ccattacagc aatgaaaaaa
     1741 taatacccgc atttaaaaac gaatacttca aagaattaat tactaaccca acagctggag
     1801 tagcgccaaa gtggttaaaa gtagattcaa aaagtttgaa gattactgtt gccgcacagc
     1861 cagcaaggga agatataaca gaagagctta atgaacaagc tgttgtggag ttctactcac
     1921 gttaagttta aaaaggttaa aaataagaat atgaacaaat tgatcattcc agcaattaaa
     1981 agtacaaaat caacaggaga taactcttct aagattacga ttagcccgct tcaaggtggg
     2041 tacggcagaa ctttagccaa cagcgttaga agggttttac tttcttctat tccaggagcc
     2101 ggcgcaaccg gtttccgaat cgaaggagtc agcaatgaat ttagcacaat tgatgg
//
LOCUS       DAOMAA010000339         2149 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_49378_length_2149_cov_2.489016, whole genome shotgun sequence.
ACCESSION   DAOMAA010000339 DAOMAA010000000
VERSION     DAOMAA010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49378_length_2149_cov_2.489016"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1243
                     /locus_tag="PLO59_05340"
     CDS             <1..1243
                     /locus_tag="PLO59_05340"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HQV00563.1"
                     /translation="EAISAGRSINLALSHRGWGGLRIAGIEDEIKKVAIPMHGRVMHA
                     IDGKISYQPYGKEGEYINSVSRGGLNCELMTLAEQHGVEINFNKRAVHVDLKNNIVFF
                     EDESGYKSQASADLIFGSDGAYSAARLQLQLTDRYNYSQSYLEHGYKELTINAGANQT
                     FKLEKNALHIWPRGGYMLIALPNLDGSFTCTLFFPHQGKPSFESLTNKAEVQNFFTSM
                     FPDAVALMPNLVNDFFSNPTGSLITVKCAPWTFENKLMLIGDAAHAIVPFFGQGMNCG
                     FEDCAVLHNLITKHNHQWNLIMAEFEKLRKPAADGIAELAVQNFIEMRDLVGKPEFLL
                     RKKIEAAFSQKFPRLWKPLYSMVTFSDIPYHQALAEGKRQDAIMEKIMALPLIETNWD
                     SDDVINKMHALCIANNDTIVL"
     gene            complement(1274..>2149)
                     /locus_tag="PLO59_05345"
     CDS             complement(1274..>2149)
                     /locus_tag="PLO59_05345"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HQV00564.1"
                     /translation="FKCVIKYLVYNGGIMRKRNKKTGHKNFKASYKKWLWAAFIFLSL
                     FTVLYFHKDIIRYGYMLSRFFEPKSKALTADVIMLPANYKVFGIDVSRYQYDIKWNAL
                     KGLDKNRDTIVFKFAIIKATEGLFWEDPTFVTNWKQAHQNKIICGAYHYFKPHVSARM
                     QALNFINSVTLQPGDLPPVIDVEEIGTLSKQELKLAVTTIATMFEKNYGIKPILYSSI
                     NFTETYLADDFKHHTFWIAHYYAPQLKISKNINWSFWQFTDAPRLIGSASYYDVNVFK
                     GTELALQKACIKKNK"
BASE COUNT          683 a          364 c          417 g          685 t
ORIGIN      
        1 agaagcgatt agcgctggcc gatcaatcaa cttagcatta agccacaggg gttggggtgg
       61 gctacgcatt gccggtattg aagatgaaat taaaaaagtt gcaattccta tgcatggcag
      121 ggtaatgcat gcaattgatg gtaaaattag ttaccagcct tatggcaaag agggcgaata
      181 tataaactca gtttcgaggg gtgggcttaa ctgcgaactt atgacactgg ccgagcaaca
      241 cggagtagaa attaatttta ataaaagagc cgtgcatgta gatttgaaaa acaatatcgt
      301 tttttttgaa gatgaaagtg gttacaaatc tcaagcaagt gcagatctta tttttggaag
      361 tgatggcgct tacagtgcgg caagattgca actgcagcta accgaccgat ataactactc
      421 gcaatcctat ttagaacatg gatacaaaga gctaaccatt aatgccggag ctaatcaaac
      481 ttttaagtta gaaaaaaacg ccttacatat ttggcctcgt ggtggttata tgcttattgc
      541 tttacctaac ttagatggta gttttacctg cactttgttt tttccacacc aaggtaagcc
      601 cagcttcgaa tcgctaacta ataaagctga agtacaaaac ttttttacaa gcatgtttcc
      661 ggatgcagtg gcattaatgc ccaatttagt aaatgatttt tttagtaatc ccaccggatc
      721 attaattacc gtaaaatgtg caccatggac atttgaaaac aagctgatgt taattggaga
      781 tgctgcccat gcaattgtgc ccttttttgg acaagggatg aattgtggtt tcgaagattg
      841 tgctgtactg cataatctga taaccaaaca caatcatcaa tggaatttga taatggctga
      901 attcgaaaag ttgcgtaaac ccgcagctga tggcattgcc gaacttgccg tccaaaattt
      961 tattgaaatg cgcgacctgg tggggaagcc tgaattttta ttgcggaaaa aaattgaagc
     1021 agcttttagt caaaaatttc cccggttatg gaaacctttg tatagtatgg taacctttag
     1081 tgatatccct taccaccaag cattagccga aggtaaaagg caggatgcta tcatggaaaa
     1141 aattatggcc ctaccattaa ttgaaactaa ttgggatagt gatgatgtta ttaataagat
     1201 gcatgcatta tgtatagcta ataatgatac aattgtgtta taagtatttt tctgaatggc
     1261 attcaaataa cagctatttg ttttttttta tacatgcttt ttgcaatgct aactcagtac
     1321 ccttaaaaac attaacatca taataagaag cgctgcctat taaccttggg gcatcggtaa
     1381 attgccagaa agaccagtta atatttttgc tgatttttaa ttggggtgca tagtaatgag
     1441 caatccaaaa cgtgtggtgt ttaaaatcgt cagctaaata agtttcggta aaattaatgc
     1501 tgctgtataa aataggttta ataccatagt ttttttcgaa catggtggct atagttgtta
     1561 cggcaagttt taattcttgt ttgcttaacg tacctatttc ttcaacgtca ataacagggg
     1621 gtaagtcgcc cggttgcaat gtaacactat taataaaatt taatgcttgc atgcgtgcac
     1681 ttacatgtgg cttaaagtag tgataagcac cacaaataat tttattttga tgagcctgtt
     1741 tccaattggt aacaaatgta gggtcttccc aaaacaagcc ttcggttgct ttaataattg
     1801 caaatttaaa tactatggta tctcggtttt tgtctaagcc ttttagtgca ttccatttta
     1861 tgtcatattg ataacgcgat acgtcaatac caaaaacttt atagtttgca ggtagcatta
     1921 taacatctgc tgtaagtgct ttcgatttag gctcaaaaaa cctgctgagc atgtaaccat
     1981 atctgattat atctttatga aagtataata cagtaaacaa cgataaaaaa ataaaagcag
     2041 cccaaagcca ttttttatag gaggctttaa aatttttatg gcctgttttt ttattacgct
     2101 ttctcattat accaccgtta taaactaaat attttataac gcatttgaa
//
LOCUS       DAOMAA010000340         2140 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_49740_length_2140_cov_3.257554, whole genome shotgun sequence.
ACCESSION   DAOMAA010000340 DAOMAA010000000
VERSION     DAOMAA010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49740_length_2140_cov_3.257554"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1301)
                     /locus_tag="PLO59_05350"
     CDS             complement(<1..1301)
                     /locus_tag="PLO59_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007811628.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HQV00565.1"
                     /translation="MQDALPYQPKNKIRIVTAASLFDGHDAAINIMRRIIQTSGAEVI
                     HLGHDRSAQELVDCAIQEDANAIALTSYQGGHNEYFKYMYDLLKERNCAHIKIFGGGG
                     GVILPTEIDALEQYGITRIYSPDDGRAMGLQGMINDLMQKSDFATGQNLNGEVKQLKN
                     NNHKDIARLISCAENFPDEFATFYPEILKAATTAQTPVLGITGTGGSGKSSLVDELVR
                     RFLIEFKTKTVAIISVDPSKRKTGGALLGDRIRMNAINNPRVYMRSLATRQSNLALSK
                     YVKEAIDIVKAANFDLIILETSGIGQSDTEITEHSNVALYVMTPEYGAATQLEKIDML
                     DFADVIALNKFDKRGALDALRDVRKQYKRNHQLWDAADEKLPVFGTIASQFNDPGMNN
                     LYRTLMNTIATKTQADLKSSYAATDEMSEKIYIIPPHRTRY"
     gene            complement(1384..1776)
                     /locus_tag="PLO59_05355"
     CDS             complement(1384..1776)
                     /locus_tag="PLO59_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00566.1"
                     /translation="MKIHLNIAFLFVSCLAISCSTNDDDPATPGTNETGFITPISQAW
                     IDTAGTNTYFFGNADSTGRFTGNETDNNGNQNFLLGKTYRFDVSFTVIKDQFSNYVDY
                     TGKLTDTSATHKRIVVYSKTDTLYLKPQ"
     gene            complement(1781..>2140)
                     /locus_tag="PLO59_05360"
     CDS             complement(1781..>2140)
                     /locus_tag="PLO59_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00567.1"
                     /translation="SKLLPQPPSDFIANISQVWIDTVDNKRKLVLANIEGSNTFMGTD
                     AFYGDEGIINLNGTLKGFKAYWTVMIKNKPKDFPRRYISYQATITDTAETHKRMIAFS
                     VLDTLYLKALPLPPLKK"
BASE COUNT          588 a          419 c          424 g          709 t
ORIGIN      
        1 aaatatcttg tacggtgagg tgggatgata tagatttttt cgctcatttc atccgttgct
       61 gcatagctcg attttaaatc ggcttgcgtt ttagtagcaa tggtattcat cagtgtgcgg
      121 tataaattat tcataccggg gtcgttaaat tgcgaagcta tggtgccaaa caccggaagc
      181 ttttcgtcag cagcgtccca aagctgatgg ttgcgtttgt attgttttcg cacatcgcgt
      241 aatgcatcta aggcaccacg tttatcaaat ttattaaggg caattacatc agcaaaatcg
      301 agcatatcta ttttttcgag ctgtgtggct gcaccatatt caggtgtcat tacatacaaa
      361 gcaacgttac tgtgttcggt tatttcagta tcgctttggc cgatgcccga agtttcgagt
      421 attattaaat cgaagttggc ggcctttact atatcaatag cttcttttac atatttactt
      481 aaagccaaat tgctttgccg ggtggctaag gaacgcatat acacacgtgg gttgtttatg
      541 gcattcatcc taattctatc gcctaataaa gcgccaccgg ttttgcgttt gctggggtca
      601 acagaaatga tggctacggt ttttgttttg aattcaatta aaaacctgcg caccagttca
      661 tcaaccaacg atgatttacc cgagccaccc gtaccggtta ttcccagcac cggtgtttgg
      721 gcagtagttg ctgcttttaa aatttcaggg taaaatgttg caaactcatc cggaaaattt
      781 tcggcacagg atattaagcg tgcgatatct ttatggttgt tgttttttaa ctgtttaacc
      841 tcgccattta aattttgacc tgtagcaaaa tcactttttt gcattaagtc gtttatcatg
      901 ccttgcaagc ccatagcgcg tccatcatcg ggcgagtaaa tacgtgtaat tccgtattgt
      961 tctaatgcat caatttcagt aggtaaaatt acaccgccac cgccaccaaa aattttaatg
     1021 tgtgcacagt tgcgttcttt aagcaagtcg tacatgtatt taaaatactc gttgtggcct
     1081 ccctggtaac tggtaagtgc aatggcattg gcatcttcct gtatggcaca gtcaaccaat
     1141 tcttgcgcgc tgcggtcatg gcccaaatga attacttcgg caccactcgt ttgaataatt
     1201 ctacgcataa tattaatggc tgcatcatgg ccatcaaata agctggctgc tgttacaata
     1261 cgtattttat ttttaggctg gtagggtagg gcatcttgca tataaaaaat attttgatgc
     1321 gaatttataa aaagcccttt ggctataaaa tggttttaat ataaccctgc tatagctatt
     1381 tttttattgt ggctttaagt ataatgtatc cgttttcgaa taaactacta tgcgcttatg
     1441 tgttgccgaa gtatcggtaa gcttaccggt ataatcaacg taattactaa actgatcttt
     1501 tataaccgta aaagatacat caaaacgata tgttttgcca agcaaaaaat tctgattgcc
     1561 attgttgtcg gtttcattac cggtaaacct gcctgtacta tcggcattgc caaaaaaata
     1621 agtgttggtg cctgctgtat ctatccacgc ttgactgatt ggagtaatga aaccggtttc
     1681 gttagtaccc ggtgtagccg gatcatcatc attagtacta cagctaattg ctaaacaact
     1741 aacaaataag aaggcaatat ttaagtgaat tttcatgact ttattttttt agtggaggca
     1801 atggcaatgc tttcaaatac aatgtatcta aaaccgaaaa ggcgatcata cgtttatggg
     1861 tttctgccgt gtcggttatg gttgcttgat aacttatgta gcgccttgga aaatcttttg
     1921 gcttgttttt aatcattacg gtccaataag ctttgaaacc ttttaaagta ccatttaaat
     1981 tgatgatgcc ttcatcgcca taaaacgcat cggtacccat aaacgtattt gaaccttcga
     2041 tattggccaa taccaattta cgtttattat ccacggtatc aatccaaact tgcgatatgt
     2101 tggctataaa atcagacgga ggctgcggta ataatttgct
//
LOCUS       DAOMAA010000341         2138 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_49841_length_2138_cov_3.640903, whole genome shotgun sequence.
ACCESSION   DAOMAA010000341 DAOMAA010000000
VERSION     DAOMAA010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49841_length_2138_cov_3.640903"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="PLO59_05365"
     CDS             <1..336
                     /locus_tag="PLO59_05365"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV00568.1"
                     /translation="ICGSHSSNQSDTLEFTTLAKREGNLEDSESDILLYPNPSKQQTT
                     LIFNSNTASTYHLTIKNYQGQIVQQQNGLVQIGENNFVIERKKLANGLYFIEFDCNNM
                     LHHSKWMLQ"
     gene            407..1396
                     /locus_tag="PLO59_05370"
     CDS             407..1396
                     /locus_tag="PLO59_05370"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HQV00569.1"
                     /translation="MKQVLLKWLLIFTSVSTLLFNSCSNELDIIAPYKDTTIVYAMLS
                     PNDSIQYIKIYKSFLGKGNAYQFAQVADSFYFPDFLTAFLVGKKNGETNDTIQLMRDT
                     LPNATYGTFASAPNIAYTTTAPISSNKSYEIIIERKNHSTVSSSTQIPRNAYLFKPNT
                     SNKEIALASDTLFSIAWQTGLNAKVYGAILRFNYLDVTPQGQTNRSIDWVLGQRIAAD
                     VYTKQDLSIDKTMADFYKLLNAKLNPPDSLSYRIAGNVEIIISGAAEDYYYYQKINNA
                     TVGVNQNIPNITNITNGLGLFSANTTNSWSFELDNLSYQYLRYSVYTAHLKFY"
     gene            complement(1453..1526)
                     /locus_tag="PLO59_05375"
     tRNA            complement(1453..1526)
                     /locus_tag="PLO59_05375"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1490..1492),aa:Pro,seq:ggg)
     gene            1717..>2138
                     /locus_tag="PLO59_05380"
     CDS             1717..>2138
                     /locus_tag="PLO59_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984937.1"
                     /note="catalyzes the hydrolysis of Xaa-His dipeptides;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HQV00570.1"
                     /translation="MSDEVRNLEPTELWNFFADLNAVPRASKKEEKIIAFIQHFGQKL
                     NLETITDAAGNVLIKKPATAGMEKCAPIVLQAHLDMVHQKNENTVFDFDTQGIEMYVD
                     QDWVNAKGTTLGADNGIGVATIMALLAATDATHPPIEA"
BASE COUNT          715 a          376 c          366 g          681 t
ORIGIN      
        1 atatgtggtt ctcattcaag taatcaaagc gatacgttag agtttacaac cttagctaag
       61 cgcgaaggaa atttggagga ttctgaatct gacattttgc tttatccgaa tccctcaaag
      121 cagcaaacaa cattaatttt taatagtaac acagcttcta cttatcacct aactattaag
      181 aattatcaag ggcaaattgt acaacagcaa aatgggttgg ttcaaattgg ggaaaacaat
      241 tttgtaatcg aaagaaaaaa gttagccaat ggtttgtact ttatcgaatt tgattgtaat
      301 aatatgttac atcacagtaa atggatgtta cagtagtaat acttttatat ctgaaaaaat
      361 gccctacctt gtgtagggca tttttattat gaatcaattt aaagtattga aacaggtttt
      421 attaaaatgg ttgctgattt tcacctctgt ttcaacactc ctttttaata gttgcagcaa
      481 tgagttggat ataattgctc cctataaaga tactaccatt gtgtatgcaa tgctttcgcc
      541 aaacgatagt attcagtata ttaaaattta caaatcattt ttaggtaagg gcaatgcata
      601 tcaatttgct caggttgccg actcatttta cttccctgat tttttaactg cgtttttagt
      661 tggcaaaaaa aatggcgaaa caaacgacac tatccaatta atgcgcgata cattgcccaa
      721 tgctacctat ggtacttttg catctgcacc taacattgcc tatactacca ctgccccaat
      781 aagttcaaac aaaagctatg aaattattat tgagcgaaaa aatcattcaa ccgtatcatc
      841 aagtacacaa ataccgcgta atgcgtattt atttaagcct aacacatcca ataaagaaat
      901 tgctttagcc agcgatacat tattttcaat agcatggcaa actggtttaa atgccaaagt
      961 ttatggtgcc attttgcgtt ttaattattt agatgtaaca cctcaaggac aaacaaaccg
     1021 cagtattgat tgggttttgg gtcaacgcat tgctgccgat gtttatacca aacaagattt
     1081 gagtatagac aaaaccatgg ccgattttta taaactgctt aatgccaaat taaatccacc
     1141 cgactcgctc tcataccgta tagcaggcaa cgttgagatt attatttcag gcgcggccga
     1201 agattattac tactatcaga aaattaataa tgccacagtg ggcgttaatc aaaacatacc
     1261 gaatataacc aacattacaa acggtttggg attgttttcg gctaatacta caaatagctg
     1321 gagtttcgaa cttgacaatt taagttatca gtacctaaga tatagtgttt atacagcaca
     1381 tttaaaattt tattaaaaat taccttattg ataaaaacgg aaaaagctct tgtaaaacaa
     1441 gagctttttt agtcggggtg actggattcg aaccagcgac caccagcacc ccatgctgat
     1501 acgctaccgg actgcgctac accccgagtt tccgtttggt aattatgtgt atttaccgct
     1561 tttaagggcg gcaaaaatat taaaatatca ttgcccatat cgtttctgta ttttttttgt
     1621 aacagacaca attattgcca attagttaag gtttagttga ttgaataatt taaaatttta
     1681 aattgttttt gtttgttttt taatcaatta tcaattatga gtgacgaagt tagaaactta
     1741 gaacctacag aactttggaa tttttttgcc gacctaaatg ccgtaccacg tgccagtaaa
     1801 aaggaagaaa aaattattgc ttttatacaa cattttggtc aaaaattaaa tttagaaact
     1861 attaccgatg ctgctggtaa tgtgcttata aaaaaaccag ctacagcagg tatggaaaaa
     1921 tgtgcaccaa tagttttgca agcacatttg gatatggtgc atcaaaaaaa tgaaaatacg
     1981 gttttcgatt ttgatacgca gggtattgaa atgtatgttg accaagattg ggttaatgcc
     2041 aaaggcacaa ctttaggtgc tgataatggt ataggtgtag ctaccattat ggctttatta
     2101 gccgcaacag atgcaactca tccacccatc gaagcttt
//
LOCUS       DAOMAA010000342         2131 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_50189_length_2131_cov_2.456647, whole genome shotgun sequence.
ACCESSION   DAOMAA010000342 DAOMAA010000000
VERSION     DAOMAA010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50189_length_2131_cov_2.456647"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2131
                     /locus_tag="PLO59_05385"
     CDS             <1..>2131
                     /locus_tag="PLO59_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00571.1"
                     /translation="MPKVIFPVSTLTCLAKGDSSVITNTTITYYPNLKKIMGSGGSVN
                     WVRGGIAPTEAYAQINNYTIDVTGSDYIIDTVTFYYKKYFDAPLLGRFTDKLLTQITF
                     ENASYPRFTSFAQTLDIKNLITDAKYRGGFALHGSRMVGGGNDRQDASLIFYRDGKPF
                     FTATSNNFTVKPDRVSSEQAAITILWNDDSIYHPGVNFKYIVADKKVTLYRDIRGAQQ
                     SPFFDTYHNVDMFFDELSWKITDPLIDLKMTVGEGEAKLTFESENYFRRQRFERIQGI
                     ADETPLYTVKKFSEKYATQTIDVKALAREMKMADDQVRSLLINLSNAGFVSFDFKTDA
                     AQIKDKLYYYLQAFTKKTDYEALQFTSQISGQPNATINLLNFDIRMRGVSQVQLSDSQ
                     NVFVVPNEQELILHQNRNFSFSGRVHAGRLDFYGSNFEFGYDDFKLDLKKIDSLRLAV
                     PTDILDDKGNPRMARVKNALQNLSGNLLIDSLNNKSGLKPGYAYPLFTATSNAYLYYD
                     SPSIKGGVYNRDKFYFKVDPFVIDSADYLLAASVLFEGNFVSNIFPDSREQLVLQPDY
                     SLGFTKLTPANGLAMYGGKATFTNKLTLSNDGLEGDGTIKYLTSTTQSKNIIFFPDST
                     LAQADLFQIDEAVLGGAEYPEVKASQVMIDFEPKKDYMDIKKTDKDFSLYQQQVALDG
                     NLRLTPQGLGGYGTSAFYDAMLVSSDFR"
BASE COUNT          649 a          409 c          434 g          639 t
ORIGIN      
        1 atgcctaagg taatttttcc ggtaagtaca ctaacatgtt tagccaaagg cgattcatcg
       61 gtaattacca atacaactat tacctattat cccaacttaa aaaaaataat gggcagtggc
      121 ggtagcgtta actgggtgcg tggaggcata gcacccaccg aagcctatgc gcaaattaat
      181 aactatacca ttgatgtaac cggcagcgat tatataattg atactgttac tttttactac
      241 aaaaaatatt ttgacgcccc attattaggt cgttttaccg ataagttgct tacacaaata
      301 actttcgaaa atgcatcgta cccgcgcttt acaagttttg cacaaacatt ggatattaaa
      361 aacctgataa ccgatgctaa ataccgtggg ggctttgctt tgcatggcag ccgcatggta
      421 ggcggtggaa acgacaggca agatgcttcg ctaatatttt atcgcgatgg caaacccttt
      481 tttacagcaa catcaaataa ttttaccgta aaacccgata gggttagcag cgagcaggca
      541 gctattacta ttttatggaa cgatgattct atttaccatc cgggcgtaaa ttttaaatat
      601 atagttgccg ataaaaaagt tacgctttat cgcgatatac gtggtgcaca gcaaagtccg
      661 ttttttgata cgtatcataa tgttgatatg ttctttgacg aattaagctg gaaaattacc
      721 gacccattaa ttgacctgaa aatgacggta ggcgagggcg aagccaaact tactttcgag
      781 tcggaaaact atttcaggcg gcaacgtttc gagcgcatac aaggcatagc tgacgaaaca
      841 cctctttata ccgtaaaaaa gttttctgaa aaatatgcta cgcaaactat tgatgtaaag
      901 gctctggcac gcgaaatgaa aatggccgat gaccaggttc gttccttatt gattaattta
      961 agcaatgccg gctttgtaag ttttgatttt aaaaccgatg ctgctcaaat aaaagacaag
     1021 ctctattatt atttgcaggc atttaccaaa aaaaccgatt acgaagcgct tcagtttaca
     1081 tcgcaaatat cggggcagcc caatgcaacc attaatttac tcaacttcga tattcgtatg
     1141 cgcggtgtaa gtcaggtgca gcttagcgat tcgcaaaatg tatttgttgt gcccaatgag
     1201 caagaactaa ttctgcatca aaaccgaaac ttctcattta gtgggcgcgt acatgcaggc
     1261 cgcttagatt tctatggcag caatttcgaa tttggttatg atgattttaa attggatttg
     1321 aaaaaaattg actcgcttcg gttagccgtt cctacggata tattagacga taaaggcaac
     1381 ccgcgtatgg cacgtgttaa aaatgccctg caaaatttat cgggcaattt acttattgac
     1441 tcgcttaaca ataaatcggg attaaaaccg ggttatgctt acccgttgtt tacagctacc
     1501 agcaatgcat acttgtatta tgattcgcct tcgattaaag ggggtgtgta taaccgcgat
     1561 aagttttatt ttaaggttga tccttttgta attgacagtg ccgactattt gttggcagct
     1621 agtgttttat ttgaaggtaa ttttgtgtct aatatttttc ccgattcgcg cgagcagtta
     1681 gtgttgcagc ccgattactc gttaggcttt accaaactta caccggccaa tggtttggcc
     1741 atgtatggtg gcaaggcaac gtttaccaac aaacttactt taagtaatga tggattggaa
     1801 ggcgatggaa ccatcaaata tttaaccagt acaacacaaa gtaaaaacat catctttttt
     1861 cctgattcaa ctttggctca agctgattta tttcaaattg atgaggccgt gttaggtggt
     1921 gccgaatatc ccgaagttaa agccagccag gttatgattg attttgaacc caaaaaagat
     1981 tatatggata taaagaaaac cgataaagat ttttcattat atcaacaaca agtggcatta
     2041 gatggcaacc tgcgtttaac acctcaaggc ttaggtggat atggcacttc agctttttat
     2101 gatgccatgt tggtatcaag cgattttcga t
//
LOCUS       DAOMAA010000343         2129 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_50286_length_2129_cov_2.894407, whole genome shotgun sequence.
ACCESSION   DAOMAA010000343 DAOMAA010000000
VERSION     DAOMAA010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50286_length_2129_cov_2.894407"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            201..1418
                     /locus_tag="PLO59_05390"
     CDS             201..1418
                     /locus_tag="PLO59_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003008730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HQV00572.1"
                     /translation="MSKISQASFASQSFLLPKESMLEIKQQQKSLFIGIPKEISFQEN
                     RIALVPEAVALLVNNGHQIMIEAGAGSNANFTDTDYSEAGAQIAYSAHEVYKADIVLK
                     IAPPAHDELAMMQDKQTLFSILQAGMQKPDYLKKMSAKRLTALGFEFIKDSTDSIPVI
                     RAMSEIVGITSILIAGEYLSNSKNGKGEILGGITGIPPTEVVVLGAGTVGEFAARTAL
                     GLGANVKVFDNNIAKLKRLQNNLGVRVYTSTISPNILGKALKTADVVVSSVTATDGRS
                     PIIVTEDMVSEMSFGSIVIDVSIDQGGNFETSEITNHTQPVFSKYGVIHYCVPNIASR
                     VARTASYALSNIFTPILMSIGDEGGLDNLLWRDAHLRHGVYMFKGTVTNKYVSEVAQL
                     PFRDLNLLIASGI"
     gene            1818..>2129
                     /gene="eno"
                     /locus_tag="PLO59_05395"
     CDS             1818..>2129
                     /gene="eno"
                     /locus_tag="PLO59_05395"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844185.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HQV00573.1"
                     /translation="MSTIAQLNARQILDSRGNPTIEVDVITNTGTLGRAAVPSGASTG
                     KHEAVELRDGDKSRYNGKGVTKAVKNVNEAIAKTLEGVSIFAQTDIDNIMLALDETEN
                     KS"
BASE COUNT          717 a          382 c          415 g          615 t
ORIGIN      
        1 tgataattat cttaatagcg gcaattattg ttttatagag tggcctcaaa atatagaaga
       61 gctattagac ttaccacata taaatttaaa tatcgaagta attctaatgc aacgcattat
      121 cacgctgcaa caagtattgt aagttaagca gtattatgtt tttggtaacc atactaatgt
      181 tagtaatatt aaattaaact atgtcaaaaa tttcgcaggc atcatttgca tcacaaagtt
      241 ttttattgcc caaagagagc atgctcgaaa taaagcaaca gcaaaaaagt ttgttcattg
      301 gcattccaaa agaaatttcg tttcaggaaa atcgaatcgc actcgtgccc gaagccgttg
      361 cacttttagt taataatggc catcaaatta tgatagaagc cggtgcaggt tcaaatgcaa
      421 attttaccga tacggattac agtgaagccg gagcacaaat tgcatacagt gcacatgaag
      481 tttacaaagc tgatattgtg ttaaaaattg caccacctgc acatgacgag ttagcaatga
      541 tgcaagataa acaaaccctt ttttcaattc tgcaagccgg catgcaaaag ccggattact
      601 taaaaaaaat gtcggccaaa cgattaactg cattgggttt tgagtttata aaggatagta
      661 cagacagcat tccggtaatc cgtgccatga gcgaaatagt gggcataaca tcaatactta
      721 ttgcaggcga atatttgagc aacagcaaaa atggtaaagg cgaaatttta ggtggcataa
      781 ccggcattcc acctacagaa gtggtggttt taggtgcagg tactgtgggc gagtttgctg
      841 cacgaactgc attgggtttg ggtgccaacg taaaagtttt tgataataac attgcaaaat
      901 taaaacgctt acaaaacaat ttaggcgtac gggtttacac atcaactatt tcgcctaata
      961 ttttaggaaa ggcactaaaa acggccgatg ttgtagtgag tagcgttacc gccacagatg
     1021 gacgctcgcc cataattgtt accgaagaca tggttagcga aatgagcttt ggctctattg
     1081 taattgatgt aagcattgat caaggtggca atttcgaaac ctctgaaatt accaatcata
     1141 cgcaacccgt ttttagtaaa tatggggtta tacactattg cgtacccaat attgcatcgc
     1201 gtgtagcacg tactgcctct tatgcactca gtaatatttt tactcctatt ttaatgagta
     1261 ttggcgatga gggcgggctt gataatttac tttggcgcga tgcgcatttg cgccacggtg
     1321 tgtatatgtt taaaggtact gtaaccaaca aatatgtaag cgaagttgcg cagttgcctt
     1381 ttagagattt aaacctgctg attgcaagcg gtatttaatt ttaaatgcat gtatcaaaca
     1441 taagcctgct attactgctt tggtttaggt ttctttaaaa atgatttaag gtatttcgat
     1501 aaagtttgtt gtaaaacctt aatacaacac gtttggctgc caacaattgt tttacgcttt
     1561 aatggcaagc attagtataa atttgctttt tttggtaagc tgccagttct gaaaaaatgt
     1621 aaatgcaatt tgcacttaac cggtaaaaag caatgcttga ttattgccaa tcaatattaa
     1681 aaaattactt tcgcacctcg aaaataaatg gcatgtttta tttataaaca tacttcaaat
     1741 aggcattgtt tttaaacccc aagcatgact catttgtttt aaaaaacaat aaaaattcaa
     1801 cattaataaa agtagctatg agcactattg cacagttaaa tgcccgacaa attttagact
     1861 cgcgtggtaa tcctacaatc gaagttgacg taataaccaa tacaggcacc ttaggcaggg
     1921 ccgcagtacc atcaggtgca tcaaccggta aacacgaagc agttgaacta cgtgatggag
     1981 acaaaagccg ctacaatggc aaaggcgtaa ccaaagcagt aaaaaatgta aacgaagcaa
     2041 tagccaaaac attagaaggc gtttcaatat ttgcacaaac cgacattgac aacatcatgt
     2101 tggctttaga tgaaaccgaa aacaaatcg
//
LOCUS       DAOMAA010000344         2118 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_50744_length_2118_cov_3.586524, whole genome shotgun sequence.
ACCESSION   DAOMAA010000344 DAOMAA010000000
VERSION     DAOMAA010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50744_length_2118_cov_3.586524"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="PLO59_05400"
     CDS             <1..380
                     /locus_tag="PLO59_05400"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQV00574.1"
                     /translation="FTGFNAGIALPLTFISNKQKVKSLALQYNALQTEADQNKLLLQT
                     QLKNAQLQYHQALIAFDFYKNTLQQAHAISNTAYTNYSNGNIGYVEYLQALENASDIK
                     LNYLKGINQINQNVLSIHYLINQ"
     gene            391..1695
                     /locus_tag="PLO59_05405"
     CDS             391..1695
                     /locus_tag="PLO59_05405"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQV00575.1"
                     /translation="MYHIYKISITFIAILLFVGCHSHDETGHDHDHNQAQPANENITA
                     ATLTADQIKAIGITTGYIEKKQLTASLKANGILKVPNQNRANVTSLMGGVINTILIQT
                     GNIVTKGQVIATLTNNSFIAMQEEYLSTLAKYDWAKADFERQQALINGNAVSQKIFQQ
                     AETDYKTLQVKKNSLQKQLMLLGINTKQLTADNIQSTINVTSPISGTASNVQVNIGSY
                     VAANNPIAEIVDNSQLHLDLYVYEKDLNKLDKGQTIHFTLTNNPGKEYDAKIYAVSNT
                     FEQNTKAVAVHATVEGNKQGLIDGMNITAIVSLENALLNAVPNDAIVNYQGLDYVFIV
                     TDKTKIADSDAVHKHKEDNENHEHEHANHQEHSHTHDAESNAVKNGETTFEKIPIKKG
                     TSDIGYTEIVLLKEIPAHARVVTKGAFFILAKMTNEGQAHAH"
BASE COUNT          816 a          398 c          340 g          564 t
ORIGIN      
        1 gttttaccgg atttaatgca ggcattgcac tgccacttac ttttattagc aataaacaaa
       61 aggtaaaatc acttgcattg cagtacaatg ctttgcaaac tgaagccgac caaaacaaac
      121 tgctgcttca aacacaatta aaaaatgctc agttacaata ccatcaagcc ttaatagcat
      181 ttgactttta taaaaataca ttacaacagg cacatgcaat tagtaacact gcatacacaa
      241 attatagtaa cggcaatatt ggatatgttg aatacttgca ggctttagaa aatgcttccg
      301 atataaaatt aaactatctg aaaggcatta atcaaatcaa tcaaaatgtt ttaagcattc
      361 attacctaat taaccaataa aagaaaacac atgtatcaca tttataaaat cagcatcaca
      421 tttattgcaa tcctactatt tgttggttgc cacagccatg atgaaaccgg gcacgaccat
      481 gaccataacc aagcacaacc agcaaacgaa aatattacag cggcaacgct tacagccgat
      541 cagattaaag ccattggaat aactacaggt tatattgaaa aaaagcaact tacagcatca
      601 ttaaaagcga atggcatttt aaaagtaccg aatcaaaatc gtgcaaatgt tacatcgctt
      661 atgggtggcg ttataaacac aattcttatt caaaccggca acattgtaac caaagggcag
      721 gtaatagcca ccctaaccaa caacagcttt attgccatgc aggaagagta tttaagcacc
      781 ttagcaaaat acgattgggc taaagccgat ttcgaaaggc agcaagcatt aataaatggt
      841 aatgccgttt ctcagaaaat atttcagcaa gccgaaaccg attacaaaac cttgcaggtt
      901 aaaaaaaaca gtttgcaaaa acagctcatg cttctgggta taaataccaa acaacttaca
      961 gctgataata tacaaagtac aataaatgtt acaagtccca tatcaggcac tgcgagcaat
     1021 gtacaggtaa acataggaag ctatgtagcc gccaacaatc ccattgccga aattgtggac
     1081 aacagccaat tacacctcga tttatatgtg tatgaaaaag atttgaataa gttagataaa
     1141 gggcaaacca tacactttac attaaccaac aatcccggta aagaatacga tgcaaaaatt
     1201 tatgccgtgt caaatacatt tgaacaaaac accaaagcgg ttgccgtaca tgcaaccgtt
     1261 gaaggaaaca aacaaggttt aattgatgga atgaatatta cagccattgt aagcttagaa
     1321 aatgcattgt taaatgctgt tcctaacgat gctatagtta actatcaagg cttggattat
     1381 gtatttatag taacagataa aacaaaaatt gcagatagtg atgcagtaca taagcataaa
     1441 gaagacaatg aaaaccatga acatgaacat gccaaccacc aggagcatag ccatacacat
     1501 gatgcagaaa gtaatgcagt aaaaaacggg gaaacaactt tcgaaaaaat tcccattaaa
     1561 aaaggaacca gcgatattgg ttatactgaa atagttttat taaaagaaat tccggctcat
     1621 gcccgggttg taactaaagg tgcatttttt atattggcaa aaatgaccaa tgaaggtcag
     1681 gcgcatgcac attaattcta tgacacatca aaagtcaacc tccatgcttg atatacattt
     1741 tcaattacaa taaaaaacag cacactcatt ttaaaattgt atctataaaa ttagcattac
     1801 ttaaaaaaaa cgccactgca acaatgtttt gcagtggcgt ttttttttgt tgttatatct
     1861 atggttgggt tagtgtcata taccacttgt caatgtaatt acaaacaagt attaaaccat
     1921 atatgtaaca cccttgcaca acaataccgg gcaccataca tagtttagtg aagtagtaac
     1981 gggtaaaatg aacaagcata aatattttac tctttaataa acttatggta aatcaactca
     2041 tcggccgtag ttattttaat ctgataaaca cccgtttgta aagttgaaac attaaacatc
     2101 tggctattgg tatttttt
//
LOCUS       DAOMAA010000345         2115 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_50877_length_2115_cov_2.833981, whole genome shotgun sequence.
ACCESSION   DAOMAA010000345 DAOMAA010000000
VERSION     DAOMAA010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50877_length_2115_cov_2.833981"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1158)
                     /locus_tag="PLO59_05410"
     CDS             complement(<1..1158)
                     /locus_tag="PLO59_05410"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HQV00576.1"
                     /translation="MNYILRCFKWLTTIVLVLFYNTLQAQLYFQQTTNYKMDVTLDEV
                     KHELNGTVTITYLNQSPADLEFLYIHLWPNAYKNNQTALCKELVKQGNKQLYFAPDSI
                     RGFIDKLQFESNHQKLKLEIDSTYEDIAKLQLSEPLKAGDSIIITTPFRLKIPSAAFS
                     RMGYYKNFYALTQWYPKVAVYDAQGWHVMPYSDQGEFYSDFGTYTVTVTLPADYVVGA
                     TGNLITASEITWLDNKANAPATNSIAIAKTQNKKTITFEAYRVHDFAWFASNQFNVVK
                     DSVLISNKWVTTWCLYNEIEKQYWQNATTYIHDALRFYSAEIGAYPYQQITVCSGTLM
                     AGSGMEYPQITIIGKAYSDYQLETTIVHEIGDNWFQGILATTERNEPWLDEG"
     gene            complement(1263..>2115)
                     /locus_tag="PLO59_05415"
     CDS             complement(1263..>2115)
                     /locus_tag="PLO59_05415"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV00577.1"
                     /translation="TLYGNTSGGVSYNIKDTIVSLARVELASAFFIPVPGPKMIWQFG
                     ELGYPYSINYCQNGTINTNCRVDPKPIKWNYYSEVARKRLYDITSALNRLKTNEAVFS
                     TATYNIQFNGQFKRYHLNHVSNNVTILGNFGVFPGTIDPNFQSTGWWYEYFTGDSIMV
                     SNVNAPINLFQGEYRLYSKNKLTTPLTTAIADNNLQTNVYPNPATNDVNVQFYTKSSS
                     PITIQILDLNGKVILSDTNENVICGQQHFTWNLTTSQGSSVNNGIYFIKVVQDSNQQT
                     AKIIVQK"
BASE COUNT          622 a          367 c          404 g          722 t
ORIGIN      
        1 gccttcgtca agccagggtt cgttacgttc agtggtagct aaaatgccct gaaaccagtt
       61 atcacctatt tcatgtacga tggttgtttc gagttgataa tcactgtaag ctttgccaat
      121 aatggttatt tgcggatatt ccataccact gccggccata agtgtaccac tgcaaacagt
      181 tatttgctgg taagggtatg caccaatttc ggcactataa aagcgtaatg catcatgtat
      241 ataggttgtt gcattttgcc agtattgttt ttcaatttcg ttatacaagc accaggtagt
      301 tacccattta ttacttatta gtacgctatc cttaacaaca ttaaattggt tgctggcaaa
      361 ccacgcaaaa tcgtgtacgc ggtaagcttc aaaagttatt gtttttttat tttgagtttt
      421 agctatagca atagagtttg ttgctggtgc attggcttta ttatccagcc aagtaatttc
      481 tgatgctgta attaaatttc cggtggcgcc aactacataa tcggccggta aagttactgt
      541 taccgtatag gtaccgaaat cgctgtaaaa ctcgccctga tctgaatagg gcatgacatg
      601 ccagccttgt gcatcataaa cagctacttt tgggtaccat tgtgttagtg cataaaaatt
      661 tttatagtac cccatgcgcg aaaacgcagc agatggtatt tttaatctga agggcgtggt
      721 aatgattatt gagtcgcccg ctttaagtgg ctctgacaat tgcagtttgg caatgtcttc
      781 gtatgttgaa tctatttcga gttttaattt ttggtggttt gattcaaatt gtagtttatc
      841 aataaaacca cgtatgctat caggggcaaa atataattgc ttattgccct gttttactaa
      901 ctctttgcac aaagcggttt ggttattttt ataggcattt ggccacagat ggatatataa
      961 aaattctaaa tcagcgggcg attgatttaa ataagtaatg gttactgttc cgtttaattc
     1021 atgctttact tcatccagtg ttacatccat tttataatta gttgtttgct gaaaatataa
     1081 ttgagcctgt aaagtgttat aaaacaaaac aagcacaatg gttgtaagcc atttgaaaca
     1141 tcgaagtata taattcatgt ataggtgtac ttagcaataa ggctgcaatt tgtgtaaaaa
     1201 caaaaacacc tgttacataa agttgaaaca ggtgttttaa aaaaaacaat tttaacggac
     1261 tgctattttt gaacaataat tttagcggtt tgctgattac tatcctgcac aacttttata
     1321 aaataaattc cattattaac actgctacct tggcttgtgg tcaaattcca ggtaaagtgt
     1381 tgttgtccgc aaattacatt ttcattggtg tctgaaagaa ttactttgcc atttaaatct
     1441 aaaatttgaa tggtaatagg cgaactgctt ttggtataaa actgcacgtt aacgtcatta
     1501 gttgcagggt taggataaac gtttgtttgc aaattgttat cagctattgc tgtggttagc
     1561 ggtgtcgtaa gtttattttt agaatataac ctgtactcgc cctgaaacaa atttataggt
     1621 gcgtttacat ttgataccat tatactgtca ccggtaaaat actcatacca ccaaccggtg
     1681 ctttgaaagt taggatcaat tgtgcccgga aacactccga aatttccaag tatagttaca
     1741 ttgttgctta catggtttaa atgatagcgt ttaaactgcc cattaaactg tatgttatac
     1801 gtagcggttg aaaacacagc ttcattggtt tttaatcggt ttaatgccga agtaatatca
     1861 taaagtcgtt tacgtgcaac ttcactatag taattccatt taataggttt tgggtcaaca
     1921 cggcagttag tatttatggt tccgttttga caatagttaa ttgagtaagg ataacccaat
     1981 tcgccaaact gccaaatcat ttttggcccc ggtaccggta tgaaaaatgc cgaagctaac
     2041 tcaactcgcg ctaatgaaac aattgtgtct tttatattat aggaaacacc gcctgatgta
     2101 ttgccatata gtgta
//
LOCUS       DAOMAA010000346         2109 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_51144_length_2109_cov_2.708861, whole genome shotgun sequence.
ACCESSION   DAOMAA010000346 DAOMAA010000000
VERSION     DAOMAA010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51144_length_2109_cov_2.708861"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="PLO59_05420"
     CDS             <1..847
                     /locus_tag="PLO59_05420"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HQV00578.1"
                     /translation="EVEIVNYYDNCQIDYEIVWHLNTHMCMHYGYWDSNTKNLRAALN
                     NMNVQVAKLVNPMPDMHVLDAGCGVGGSSVFLAKNFGCKTTGITLSDKQVQTCFSNAI
                     KCGVAHLSHFEKQNYLNTHFADNTFDVVWAIESVCYAFDKADFIKEAYRILKPGGKLV
                     VADFFNYKVQPNTRQHLLMQKWTSTWAIKQYADVDEFYKKLSNCGFVNCTQKDVTAQV
                     IKSIKRLYYSFFPGLPITYISQWLGFRNRTQTANTWSTYYQYKAYKQNLWRYMFYTGT
                     KPKNN"
     gene            966..>2109
                     /locus_tag="PLO59_05425"
     CDS             966..>2109
                     /locus_tag="PLO59_05425"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HQV00579.1"
                     /translation="MTNPTPILSHPDFDKWFATFRQMQDPEADKVALALVASPYGHQA
                     YQILASISKNSDTIDERKFADLTQRMQKSTDSGEHANIVTILNNYFADTSHFNFTDAQ
                     KQRIKNACFFFNNYAIEGTMILAVRSLLKQYAAYNSTQVLGSTRMLPQYPHRRILATM
                     DFVLDVMDPNAFEPNGSAIKSIQKLRLVHAMIRARINLKSAVEEKKISIKEDVEISTD
                     FWNSNVWQKEKWGAPINQQDMLFAINTFSQEVIDGIISSGEKPDDEVLNDYYYTWHLY
                     GRALGLHDEINPTTYAEGKALQEKIYQVQFYPKNADGTPFINPIAPALSAPLVSFIKE
                     FSGLPKISYVYAIIRRYNSDIDETFFENVHKVPMQEKSKFFLILMVI"
BASE COUNT          746 a          380 c          390 g          593 t
ORIGIN      
        1 cgaagttgaa attgtaaact attacgacaa ctgtcaaatt gattatgaaa tagtatggca
       61 cctaaataca cacatgtgta tgcattatgg ttattgggat agcaacacca aaaatctgag
      121 ggcagcatta aataacatga atgtacaagt agccaaactt gtaaacccga tgcccgatat
      181 gcatgtgttg gatgctggtt gcggtgtggg aggctcttct gtttttttag caaaaaattt
      241 cggttgcaaa actacaggca ttacgcttag cgataagcaa gtacaaacct gttttagtaa
      301 tgcaataaaa tgcggtgtag cacacctatc acatttcgaa aaacaaaact atttaaacac
      361 acattttgcc gataatactt ttgatgtagt gtgggctatc gaaagcgttt gttatgcttt
      421 tgacaaagcc gattttataa aagaggccta tcgtatttta aaacccggtg gtaagttggt
      481 ggtagccgat ttttttaatt ataaggtaca gcccaacacc cggcaacatc tattaatgca
      541 aaaatggaca agcacctggg ctataaaaca atatgccgat gttgatgagt tttataagaa
      601 actaagcaat tgtggatttg taaattgtac acaaaaagat gtaacagcac aggttataaa
      661 aagtataaaa cgcctgtatt attcattttt tccgggctta cccataacct atataagcca
      721 atggctggga tttagaaacc gaacccaaac agcaaacacc tggtcaacct attatcagta
      781 taaagcctat aaacaaaact tatggcggta tatgttttat accggcacta aacctaaaaa
      841 caattaatta atctttagcc tgcattaaca caaccgttaa aataaaccaa cgctttttga
      901 aacacgtact gcctaccatt acaataatac ccctattgtt taaaaaccaa taattgtaag
      961 catctatgac taatccaacc cccatattat cacaccctga tttcgataaa tggtttgcaa
     1021 cattcaggca aatgcaagac cctgaggccg ataaagtagc attggctttg gttgcatctc
     1081 cttatggaca tcaagcctac caaattttag cttctataag caaaaacagc gatacaattg
     1141 atgaaagaaa gtttgcagat ttaacacaaa gaatgcaaaa aagcacggat agcggtgagc
     1201 atgcaaacat tgtaaccatt ttgaataatt attttgccga taccagtcat tttaatttta
     1261 cggatgcgca aaaacaacgg ataaaaaatg catgtttttt ttttaataat tatgccatag
     1321 agggtaccat gattttggct gtacgctcat tgctaaaaca atatgcagca tacaacagta
     1381 cacaggtttt aggtagcaca cgtatgttgc ctcaataccc acacaggcgc atattagcta
     1441 ctatggattt tgttttagat gtaatggatc caaatgcatt tgagcccaat ggcagcgcca
     1501 taaaaagtat tcagaaatta cgattggtgc atgctatgat aagagcacgt ataaatttaa
     1561 aatcggctgt agaagaaaaa aaaatcagta taaaagagga cgtagaaata agcacagatt
     1621 tttggaacag caatgtttgg caaaaggaaa aatggggcgc accaattaat caacaagata
     1681 tgttgtttgc cataaatacg tttagtcagg aagttataga cggcattatc agttcgggcg
     1741 aaaagccgga tgatgaagta ttaaacgatt attattatac ctggcattta tacggacgcg
     1801 ccttaggttt gcatgatgaa attaatccaa caacttatgc cgaaggcaaa gctttgcagg
     1861 aaaaaatata tcaagtacag ttttacccaa aaaatgccga tggtacacca tttataaacc
     1921 caattgcacc ggcattatcg gcaccgttgg ttagttttat aaaagagttt tcgggcttgc
     1981 ccaaaataag ttatgtatat gccatcatac gcagatataa cagcgacatt gatgaaactt
     2041 ttttcgaaaa cgtacacaaa gtacccatgc aggaaaagag caagttcttt ttaatattga
     2101 tggtaattg
//
LOCUS       DAOMAA010000347         2103 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_51421_length_2103_cov_2.672852, whole genome shotgun sequence.
ACCESSION   DAOMAA010000347 DAOMAA010000000
VERSION     DAOMAA010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51421_length_2103_cov_2.672852"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="PLO59_05430"
     CDS             <1..185
                     /locus_tag="PLO59_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008240228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HQV00580.1"
                     /translation="EITTGEQVEAWAGGHENYAYQENNGITTVIVEIDTIDEYLDYFN
                     NTYPLALDKLKNIAEQ"
     gene            complement(262..1200)
                     /locus_tag="PLO59_05435"
     CDS             complement(262..1200)
                     /locus_tag="PLO59_05435"
                     /inference="COORDINATES: protein motif:HMM:NF016103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HQV00581.1"
                     /translation="MKTIQFMTSLRLNKLTCYLTLWLCICAPTLLAQLNHTDYKLYAT
                     ATKSIIDLDSLINSCAGADVIFFGEEHNDSVAHFLETEIWKKLHNRYQRVCLSLEMFE
                     RDVQPTMNDYLSGLIREKNFLKDCRVWNNYRDYKPMVEYAKQNKLPVICANAPGRYSN
                     MAGRLGPGSLSEIDATSKKYIAPLPYALAGGGYFEKLTALTSHGKDSTPMPATMNNFN
                     MIAAQSLWDATMAYSIAQGLKAHKKTKVFHVNGRFHSDEHYAVVTQLKNYNAKAKCMV
                     ISCFRDDAFNEPNWEAWHKLGDYIIVTNPAVPTTYE"
     gene            complement(1160..>2103)
                     /locus_tag="PLO59_05440"
     CDS             complement(1160..>2103)
                     /locus_tag="PLO59_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00582.1"
                     /translation="LYCLLMNILNGKLPALLGFLFFTAIALLSKLPSCIVLICVVPMV
                     YYSKLNPTKKVMLYAIFSIACLPAVWWYFIWVPHLVKTFGFWHFFMGTTWQQGFAEIA
                     ANKLLTASRFYDSALKFTGFALFVWGCICMFYKKHYVSVIALVSSLIITLALVFKAGF
                     TFAHHSYYIIPFVPAMALTAGYGLTSIRQKKLAIFCLAVVCIEGVLNQYHDFKVKPNE
                     AAVLNLENDLNKIEAKGLIMINSGDNPTPMYFAHRKGWIAHNYQIADTSFINQCKTNG
                     LKQLVILQHAFGTNISVPYTKLFTNENYTIYDIASAK"
BASE COUNT          667 a          402 c          392 g          642 t
ORIGIN      
        1 ccgaaattac aacaggcgaa caagttgaag cctgggcagg tggccatgaa aattatgcct
       61 atcaggaaaa taacggcatt acaaccgtaa ttgttgaaat tgacacaata gacgaatatt
      121 tagactactt taataatacc tatccactgg cattagataa gttaaaaaat attgctgagc
      181 agtaatttag cttgctgtag ttgtaaaaat taatctgtta tttaattact acaaaacttt
      241 aaatctgacg gagttcgttg tttattcata agtggttggt actgccggat tggttacaat
      301 aatataatcg cccaatttgt gccaagcttc ccagttgggt tcattaaatg catcgtctct
      361 gaaacatgag ataaccatac acttagcttt tgcattgtaa ttttttagtt gcgttactac
      421 ggcataatgc tcatcgctgt gaaagcggcc atttacatga aacacctttg tttttttatg
      481 cgcttttaaa ccctgggcaa tgctatatgc catcgtagca tcccaaagtg attgtgcagc
      541 aatcatatta aaattgttca tcgtggcagg catgggtgtg ctatctttac catgcgaggt
      601 taatgccgtt agtttttcga aatagccgcc accggccagt gcatagggta ggggcgcaat
      661 atatttttta ctggttgcat caatctcact caaactgcct gggcctaaac ggccggccat
      721 gttactatac cggccgggag cattggcgca tattacaggc agtttgtttt gcttagcata
      781 ttcaaccatg ggcttataat ccctgtaatt attccacaca cggcagtctt ttaaaaaatt
      841 tttttcacgt atcaatccac ttaaataatc attcatggtt ggttgtacat cacgctcaaa
      901 catttctaac gacaggcata cacgctgata ccggttatgc agttttttcc atatttcagt
      961 ttccagaaaa tgtgcaactg agtcgttatg ttcttcgcca aaaaaaatta catcagcacc
     1021 tgcacaacta tttattaaac tatctaaatc aataatactt ttagtagccg tagcataaag
     1081 tttataatcc gtatggttaa gttgcgccaa tagcgtgggg gcacagatgc ataaccataa
     1141 agttaggtag cacgttagtt tatttagccg aagcgatgtc ataaattgta tagttttcat
     1201 ttgtaaaaag tttcgtgtaa ggaactgata tgttcgttcc aaaagcgtgt tgcaaaataa
     1261 ccaactgttt taatccattt gttttacatt gattaataaa tgaggtgtcg gctatttgat
     1321 agttatgtgc aatccaccct ttacgatgtg caaagtacat gggcgtagga ttgtcgccac
     1381 tgtttatcat aattaatcct ttggcttcaa ttttatttaa gtcgttttct aaattaagca
     1441 ctgcggcctc attaggtttt actttaaagt cgtgatattg atttaaaaca ccttcgatgc
     1501 aaaccacagc caggcaaaag atggcaagct ttttttgcct gatgctggta aggccatagc
     1561 ccgcagttag agccattgcc ggcacaaacg gaataatgta gtagctatga tgtgcaaaag
     1621 taaatcctgc tttaaacacc aaggctaaag ttataattaa ggatgaaacc aatgcaatca
     1681 cacttacata gtgtttctta taaaacatac aaatacaacc ccaaacaaat aatgcaaaac
     1741 ctgtaaactt taaagcgcta tcataaaaac gcgatgccgt aagtaattta tttgcggcaa
     1801 tttctgcaaa gccctgctgc caggttgtgc ccataaaaaa atgccaaaag ccaaaagttt
     1861 taaccaggtg gggtacccat ataaaatacc accaaacagc aggtaggcag gctattgaaa
     1921 aaatagcgta tagcattact ttttttgtgg gatttaattt gctgtaatac accattggca
     1981 caacacaaat tagtacaatg caactgggta gtttgcttaa caaagctata gcagtaaaaa
     2041 ataaaaagcc caaaagtgca ggtagtttcc cattaaggat attcattaat aagcaataca
     2101 aag
//
LOCUS       DAOMAA010000348         2101 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_51511_length_2101_cov_2.839687, whole genome shotgun sequence.
ACCESSION   DAOMAA010000348 DAOMAA010000000
VERSION     DAOMAA010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51511_length_2101_cov_2.839687"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..112)
                     /locus_tag="PLO59_05445"
     CDS             complement(<1..112)
                     /locus_tag="PLO59_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015265309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HQV00583.1"
                     /translation="MKITIDNNSGFCFGVVFAIQMAEDELDRTGHLYCLGD"
     gene            complement(119..838)
                     /gene="cmk"
                     /locus_tag="PLO59_05450"
     CDS             complement(119..838)
                     /gene="cmk"
                     /locus_tag="PLO59_05450"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HQV00584.1"
                     /translation="MLPASPNNRKLVIAIDGYSSCGKSTLAKDVALALQIKYVDSGAM
                     YRAVALYFIENHIHIPTEQSTQSVASVLQKINLDFFFNHALQQFEITLNGKFVEHEIR
                     SMHVADVVSNVSALKEVRSFLIAKQQELGSNGGVVMDGRDIGTAVFPNADLKFFMTAK
                     PEIRAQRRLKELQNKNLQVSFEEVLANLHQRDLLDTTRKTNPLTCAADAIVIDNSHLT
                     RQEQLQIVLDKVRNLQTVQIK"
     gene            1071..>2101
                     /locus_tag="PLO59_05455"
     CDS             1071..>2101
                     /locus_tag="PLO59_05455"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HQV00585.1"
                     /translation="MKKQKIFFTLLVAGQMVTTVYAQNWLTSGNLSTKTDTLGTRTNQ
                     SVNIITNNASRMFIAKNGNVGIGTTAPAFLFDVQGPGNASMSFKSTTGTANVILDRFN
                     STSTAGVNYRTNGVPYWQTGTINNDNFVIRNLNLAAPALAIMYATSNVGIGNNVPTAK
                     LDVTGGTTAADTIATINATVGYVGAFDVPAITALSEPDAGYGIGVAAAGGFIGVSGAS
                     TFIGSIGYADAGSTSGGTVYGIYGEAGIATSSVGVFGTAGDGTNQYGVYGITANGLAD
                     TVGTTKWAGYFVGDVAGARYFNFSDGKLKANIQPINNALNSLLKINATSYDFKCSEYP
                     SLALPAGKQ"
BASE COUNT          630 a          366 c          404 g          701 t
ORIGIN      
        1 tatcgcctaa acaatataaa tggcctgttc ggtctagttc gtcttcggcc atttgaatgg
       61 cgaaaacaac tccaaaacaa aaccccgaat tgttatcaat tgtaattttc attatcactt
      121 attttatctg aacagtttgc aaattacgta ctttatctaa aacaatttgt aattgttctt
      181 gtcgggttaa gtggctgttg tctatcacaa ttgcatcagc ggcacatgtc aaaggatttg
      241 ttttacgtgt tgtatctaac aagtcgcgtt ggtgtaaatt tgccagcacc tcttcaaaac
      301 ttacctgtaa atttttgttt tgtagttctt tcagccttcg ttgggcacgt atttcaggtt
      361 ttgctgtcat aaaaaatttt aaatcggcat ttggaaatac tgctgtgcct atatcacgcc
      421 catccataac aacgccacca ttgctaccca gttcttgttg tttagcaatt aaaaacgaac
      481 gaacttcttt caaagcactt acattactta caacatctgc aacgtgcatg gatctgattt
      541 catgctcaac aaatttacca tttaaagtaa tttcaaattg ttgaagggca tggttaaaaa
      601 agaaatctaa atttattttt tgtaatacac ttgctacgct ttgtgtactt tgttcagtag
      661 gaatgtgaat gtggttctcg ataaaataaa gtgcaacggc acggtacatg gcacccgagt
      721 caacatattt tatttgtaaa gctaatgcta catcttttgc aagggtactt tttccgcatg
      781 atgaatatcc atcaatggca ataactaatt ttctattgtt tggagatgca ggtaacatgt
      841 tgcaaaagta gcgttttgag ctgtgttgca atgttgataa caatattttt tcaggttgag
      901 taattggcta ttttgcaagc atgctattaa aattatattt taattttttg gaatattttt
      961 aatggcttat attaactgta gatatcattt tacaactaat atctttcttg taaacgttat
     1021 attgaaattg ttaattcctc aatatttatt tcacttaaaa acaaacaatt atgaaaaaac
     1081 aaaaaatctt ttttacgcta ttagttgccg gacaaatggt aactaccgtg tatgcacaaa
     1141 attggcttac atcgggtaat ttaagtacaa aaaccgatac cttaggtact cgcaccaatc
     1201 aaagtgtaaa cattataacc aataatgcca gccgcatgtt tattgcaaaa aatggcaatg
     1261 taggtattgg tactacggca ccggcatttt tatttgatgt acaaggccca ggtaatgcca
     1321 gtatgagttt taagagtaca acaggcacag ccaatgttat tttagacagg tttaattcta
     1381 cgagtacggc cggagttaat tatcgtacca atggggtacc atattggcaa accggtacaa
     1441 ttaacaatga taattttgta ataaggaatt tgaatttagc tgcgcctgca ttagccataa
     1501 tgtatgcaac gagtaatgtt ggtataggca acaatgtacc cacagctaaa ttagatgtta
     1561 caggtggcac aactgctgcc gataccattg ctaccataaa tgcaactgta ggttatgtgg
     1621 gtgcttttga tgtgccagca attacagctt taagcgagcc tgatgccggc tatggtattg
     1681 gcgtagcagc agcaggtggt tttattggcg tatcgggcgc atcaacgttt attggcagta
     1741 ttggttatgc tgatgcaggc tcaacatcag gtggcaccgt ttatggtatt tatggcgagg
     1801 caggcatagc aacttcttca gttggtgttt ttggtactgc tggcgatggt accaatcagt
     1861 atggtgttta tggtataact gcaaatggtt tagctgatac agttggcaca actaaatggg
     1921 caggttattt tgtaggcgat gtagccggag cgcgttactt taattttagt gatggtaaac
     1981 ttaaagccaa cattcaacct attaataatg cattaaacag tttacttaaa attaatgcca
     2041 catcttatga ttttaaatgc agcgaatatc caagtttggc tttacctgca ggcaaacaat
     2101 t
//
LOCUS       DAOMAA010000349         2095 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_51776_length_2095_cov_3.026961, whole genome shotgun sequence.
ACCESSION   DAOMAA010000349 DAOMAA010000000
VERSION     DAOMAA010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51776_length_2095_cov_3.026961"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..1891)
                     /locus_tag="PLO59_05460"
     CDS             complement(14..1891)
                     /locus_tag="PLO59_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HQV00586.1"
                     /translation="MKNSNASLAEVNATVPTKVAGAKWRTWFAFFGPAYLVSVGYMDP
                     GNWATDIAGGSKFGYSLLWVLLMSNLIALLLQSLSARLGIVRGLDLAQASRQMYPKAT
                     NLFLYVLAEIAIAACDLAEVLGMAIGLQLLFDIPLIYGVCICVLDTFLLLFLLSFGMR
                     KLEAFIICLISIIGFAFLSQLIMAKPDLTAVAKGFIPAIPTEQALYIAIGIIGATVMP
                     HNLYLHSSLVQTRKFDLTPASIARAIRLNFFDSAIALNLALFVNAAILILAATVFFKN
                     GLYEVAEIQDAHKLLQPMLGSTWAPTLFAIALIAAGQSSTITGTLAGQIIMEGYLQLR
                     IQPWVRRLITRLIAVVPALITILYAGESATGNLLILSQVILSMQLGFAIIPLIHLTSD
                     RIKMQQFAISNWVKTLAWISASIIVALNVKLVYEQINIYTTEHPHNIWMQALVIPFLL
                     FAFGMLLYITIYPYFTNKQAQDAKGIHGTFTELTLPPVSTFKRIAIAVDFTKADEKTI
                     QHALQQGGLNASYLLIHTVETAGAFIIGANIKDAESESDAQLINHYEMHLKEKNYQVV
                     STIGYGNPKKAIPQLTIAFDADLLVMGSHGHRAFSDIIWGTTIDAVRHQLNIPVLVVR
                     L"
     gene            complement(1892..>2095)
                     /locus_tag="PLO59_05465"
     CDS             complement(1892..>2095)
                     /locus_tag="PLO59_05465"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HQV00587.1"
                     /translation="SLATIVAVTNDDVRFLKQLEKLNLTIGTKITILAKHDFDNAMEI
                     KPMRKPAVTISEALAKNILIQLN"
BASE COUNT          659 a          402 c          409 g          625 t
ORIGIN      
        1 cgcttttggt aaattataac ctaacaacta aaaccggaat gtttagctga tgcctaaccg
       61 catcaatggt agtgccccaa ataatatcac taaacgccct gtgcccgtgc gaacccataa
      121 ctaataaatc ggcatcgaat gcaatggtaa gttgtggtat tgctttttta ggattgccat
      181 aaccgatggt tgaaaccacc tgataatttt tttcttttaa atgcatttca taatgattta
      241 taagctgtgc atcgctttct gattcggcat ctttaatgtt agcaccgata ataaatgcac
      301 cagccgtttc aacggtatgt ataagcaaat aagatgcatt taagccacct tgttgtaaag
      361 catgttgtat ggttttttca tctgccttgg taaaatcaac ggcaatggct atgcgcttaa
      421 aagttgaaac cggtggcaat gtaagttcgg taaaagtgcc atgaatacct tttgcatcct
      481 gcgcttgctt atttgtaaag taaggatata tagtaatgta taggagcatc ccaaacgcaa
      541 aaagcaaaaa cggtattacc aatgcttgca tccaaatgtt atgtggatgt tcggtagtat
      601 atatattaat ttgttcatat accagtttta catttaaagc aacaatgatg ctggcgctaa
      661 tccaagctaa tgttttaacc caatttgaaa tagcaaactg ttgcatttta atacggtcgc
      721 tggtaaggtg tattaatggt ataatggcaa accctaattg catactcaat ataacctgac
      781 tcagtataag taaattaccc gttgccgact cgcctgcata tagtatggta attaaagctg
      841 gtacaactgc aattaaacgt gttattaatc ttctaaccca gggctgtatg cgcagttgaa
      901 ggtaaccttc cattattatc tgtccggcta aggtgcctgt aatggtactg ctttgtccgg
      961 ctgcaattaa tgcaatggca aacagggtag gtgcccacgt tgagcctaac atgggttgta
     1021 ataatttatg agcatcctga atttcggcaa cttcgtacaa accattttta aaaaatacag
     1081 tagcagctaa tatgagtatg gcagcgttta caaacagagc caggtttaaa gcaatagccg
     1141 aatcgaaaaa atttaaacga atagcgcgcg caattgatgc cggtgttaaa tcaaatttgc
     1201 gtgtttgtac caaggaggag tgtaagtata agttatgtgg cataacggtg gcgccaataa
     1261 taccaatggc aatgtatagt gcttgttcag taggtattgc cggaataaat cctttggcta
     1321 cggccgttaa atcgggtttg gccataatga gctgagacaa aaatgcaaaa ccaataatac
     1381 ttatcaggca gataataaat gcttcgagtt tgcgcatgcc aaagcttaac aggaagagca
     1441 gtaaaaatgt atctaacaca caaatgcaaa caccataaat taatggtatg tcaaaaagta
     1501 attgcaagcc tatggccatg cctaacactt cggctaaatc acatgcggca atggcaattt
     1561 cggccaatac gtacaaaaac aaattggtgg ccttggggta catttgacgc gatgcctgtg
     1621 ccaaatctaa accacgtaca atacctaaac gtgcgcttaa actttgcagt aacagtgcaa
     1681 ttaaattgct cattaaaagc acccataaca atgaataccc aaatttacta ccccctgcaa
     1741 tatcagtagc ccagttgccc gggtccatgt atccaacact tactaaataa gcaggaccaa
     1801 aaaaagcaaa ccaggtacgc cattttgcac cggctacttt ggtaggtacg gtagcattta
     1861 cttcggctaa cgatgcattg ctgtttttca tttaatttaa ttgtattaaa atatttttgg
     1921 ctaatgcttc gctaatggtt acagccggtt tgcgcatagg tttaatttcc attgcattgt
     1981 caaaatcgtg cttggctaat atggttattt ttgtgccaat ggttaagttg agtttttcta
     2041 attgttttaa aaatcttaca tcatcatttg ttacagcaac aatggttgct aaact
//
LOCUS       DAOMAA010000350         2094 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_51832_length_2094_cov_2.351153, whole genome shotgun sequence.
ACCESSION   DAOMAA010000350 DAOMAA010000000
VERSION     DAOMAA010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51832_length_2094_cov_2.351153"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="PLO59_05470"
     CDS             <1..771
                     /locus_tag="PLO59_05470"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQV00588.1"
                     /translation="ELLQMELSTMNARSNLAQAQLDRETHELRLRNFLNIKGQQHITL
                     VEPPLIPDFVVDDSIALMQARQNRQAVVEFERRRIEADRDVAQARGNNGFNADIVATY
                     GLTNTAGTLSDVYKNSLEQERINLSFQLPILDWGRTRSQVRTAQVSRELTRTILERDQ
                     QNFEQEVVLLAKQFSMQRAKLIIATRADTIAQKRYEISMKRYLIGKIDITDLNIALQE
                     KDNAKVQYLASLQQFWSSYFDLRRKTLYDFENNKTITN"
     gene            complement(780..2051)
                     /locus_tag="PLO59_05475"
     CDS             complement(780..2051)
                     /locus_tag="PLO59_05475"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HQV00589.1"
                     /translation="MNNLYLILSTLIFTAFFAGIELAFVSSNKFRIELDNAQGRFSAR
                     ILSFFTKSPSRFIITALIGINISLVIYSNSIETVLEPYVLTYLPLQYHNSFIILAIKT
                     ILSTAIILIAGEFLPKVLFRINPNQSLNFFATIFYVFYILFYPLVFLILKLSHLIMQV
                     FFKLDFVEDKPVFGRIDLDHYIHELNVKSNQKNELNTEIQMFQNALDFDRLKARECMV
                     PRADIVAIDINADIETLRQLFVSTGLSKILVYQQSIDYIVGFVHSFDMFKQPKSIKNM
                     MLPIAVVPETMPARSIMTTLTQQRKSVCVVVDEHGVTSGLITVEDIMEEIFGEIDDEH
                     DIEELTLKKISENEYQLSGRYEIDYLNTHLNLHIPEGDYTTLGGFIFYHHQDIPVINE
                     VIEIAPFTITIDEVINNRINLVTLKTAFPET"
BASE COUNT          701 a          378 c          397 g          618 t
ORIGIN      
        1 gaattgttgc aaatggaatt aagcaccatg aatgcacgta gcaaccttgc gcaagcacaa
       61 ttagaccgtg aaacgcatga attgcgtttg cgcaattttt taaacatcaa aggccagcaa
      121 cacattacac tggtcgagcc tccgctgatt cccgattttg tggttgacga tagtatagcc
      181 ttaatgcagg cacgtcaaaa ccggcaagct gttgtcgaat ttgaacgcag gcgcattgaa
      241 gccgatagag atgtggcaca agcgcgcggc aacaatggct ttaatgcaga tattgtagca
      301 acatacggtt taaccaatac agcaggtacg cttagcgatg tatataaaaa ttcgttagaa
      361 caggagcgca taaacttatc atttcaatta cccattttag actgggggcg cacacgttct
      421 caagtacgaa cagcacaagt aagccgcgaa ttaacgcgta ctattttaga acgagaccaa
      481 caaaattttg aacaggaagt agtgttgcta gccaagcagt ttagtatgca acgtgccaaa
      541 ttaattattg caacccgtgc cgataccatt gcccaaaagc gttacgaaat tagtatgaag
      601 cgttatttga tcggaaaaat tgatatcacc gatttaaaca ttgccttgca ggaaaaagac
      661 aatgccaaag tgcaatatct ggcctcttta caacagtttt ggagttctta ttttgattta
      721 cgcagaaaaa cgctttacga ttttgaaaac aacaaaacca taactaatta acgtgtaaat
      781 tatgtttctg ggaaagctgt ttttagtgtt actaaattaa tcctgttatt aattacttca
      841 tcaatagtaa tagtaaaggg cgctatttca atcacttcgt ttattactgg tatatcctga
      901 tgatggtaaa aaataaaacc gcctaaggtt gtataatcgc cttcgggtat atgcaggttt
      961 aaatgggtat tcaaataatc aatttcatac ctgcccgaaa gctgatattc attctcactt
     1021 atttttttta gtgtaagttc ttcaatatca tgttcgtcat caatttcacc aaaaatttct
     1081 tccataatat cttcaacggt aattaaaccc gaagtaacac catgttcgtc aaccactaca
     1141 caaacacttt tacgttgctg ggttaaggtt gtcattatac tgcgtgccgg catggtttcg
     1201 ggtacaacgg caataggcag catcatattt ttaatgcttt tgggttgttt aaacatatca
     1261 aatgaatgaa cgaagcccac tatgtaatca atagattgtt ggtacactaa aatttttgac
     1321 aagcctgtac ttacaaaaag ttggcgcaat gtttcaatgt cggcattaat atcaatggct
     1381 acaatatcgg cacggggcac catgcattcg cgggctttaa ggcggtcaaa atccaatgcg
     1441 ttctgaaaca tttgaatttc ggtgtttagc tcattttttt gattgctttt tacattcagt
     1501 tcatggatgt aatggtctaa atcaatacgg ccaaaaaccg gtttgtcttc aacaaaatcg
     1561 agcttaaaaa acacttgcat aattaaatgc gaaagtttta atatgagaaa caccaatggg
     1621 taaaacagaa tataaaacac ataaaaaatg gtggcaaaaa aattaagact ttggttaggg
     1681 ttaattctga aaagcacttt gggcaaaaac tcgcctgcta ttaagataat ggctgtactc
     1741 aaaattgttt ttattgctaa aatgataaat gagttgtgat attgaagcgg caaataagta
     1801 agcacgtaag gctctaacac tgtttcgatg ctattagaat aaataaccaa tgaaatatta
     1861 atacctatta aagcggtaat aataaaacgc gaaggcgact tggtaaaaaa cgaaagtatg
     1921 cgtgccgaaa atcgcccctg ggcattatcc aactcaattc tgaatttatt gctggaaaca
     1981 aaagcgagtt caatgccggc aaaaaaagcc gtaaaaatta atgtgcttaa aatcagataa
     2041 aggttgttca atgcagttgt tattatttct gaaatttctt aagttgtttg tatc
//
LOCUS       DAOMAA010000351         2093 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_51859_length_2093_cov_3.868008, whole genome shotgun sequence.
ACCESSION   DAOMAA010000351 DAOMAA010000000
VERSION     DAOMAA010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51859_length_2093_cov_3.868008"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1934
                     /locus_tag="PLO59_05480"
     CDS             <1..1934
                     /locus_tag="PLO59_05480"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQV00590.1"
                     /translation="EENILRHNNFLYYCHGDSLLKFDLKTHTKKLVSYSKQSRPYTFT
                     VHADSIIVGYRKKICVLRHDTIQTLIDFSKYRYSNNPFRIYVLDKDHLFFCAYKGAYI
                     CNLQTQIIDTLKPLQNQLVHNAEMHQGFLIIGTYANGFYLYINKQLVKMPLDEAGYLA
                     TTNSFLIDSQNNIWIGTNKGLYKTDFNTLIKYFYDNTTTVYYTYYNEDDGMLTSEFNG
                     GCTSSALQLKNGYASMVSMNGLVWFKPEQMQHATLNNNIYIDKIWFDNALTDKALETI
                     PKQTEHINIELATAYWGHPQNLQLAYRIKGFSKIWKPIDAERKSIYFYKPPAGNYVLE
                     IRKCIGNKANEFALLSIPFSVEQKLVESNIFIVAICLAILTFILVVVKLVTQNIQRKN
                     RLLENKVLQRTNELQVANNQLKQSIVAKDKLISIVSHEIVTPLRFLAMVAKQTGSKPM
                     SEKNWLPAMNDIKNTSESLYNNAQNILNWIKHQNKRFNVTINHIALSGFCDEITTVFN
                     EMAFAKNIHFKNEIADDTIIKSDKNILGIIVNNLFSNAVKFTENGTITITCQMQTENF
                     TLTITDTGTGINQQQMNRIHGIIQKNNTEVSFHSDSSGNNGLGYLIIVDLLSLLNGSI
                     AISSKLNEGTKVTLTLPIQ"
     gene            complement(1962..2084)
                     /locus_tag="PLO59_05485"
     CDS             complement(1962..2084)
                     /locus_tag="PLO59_05485"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00591.1"
                     /translation="MLQQTGRYKGYAKRTYDFNIYIAGYSDHFPTYLLMLKEVK"
BASE COUNT          759 a          367 c          339 g          628 t
ORIGIN      
        1 atgaagaaaa cattttgcgt cacaataatt ttctttacta ctgccatggt gatagcctat
       61 taaaattcga cttaaaaacg catactaaaa agttagtcag ttactcaaaa caaagtcggc
      121 cttacacttt tacagtacat gccgattcga taattgtggg ctatcgtaaa aaaatttgtg
      181 tattaaggca tgatactatt caaacattaa ttgatttttc gaagtaccgt tatagtaata
      241 acccattccg gatttatgta cttgataagg atcacttgtt tttttgtgct tataaaggtg
      301 cttatatctg taatttgcaa acacaaatta ttgacacatt aaaaccacta caaaaccaac
      361 tggtgcataa tgcagaaatg catcaaggtt ttttgataat cggcacttat gctaatggtt
      421 tttatcttta tataaacaag cagctagtaa aaatgccgct ggatgaagcc ggttatcttg
      481 caactacaaa cagtttttta attgatagcc aaaataatat atggataggg actaataagg
      541 gcctttataa aacggatttt aataccttaa taaaatactt ttacgacaat acaacaaccg
      601 tttactacac atactacaat gaagatgacg gcatgcttac gtccgaattt aacggaggat
      661 gcacttcatc ggcactgcaa ttaaaaaatg gttatgccag catggtttct atgaatggct
      721 tggtatggtt taagcccgag caaatgcaac atgctacact taataacaat atatacatag
      781 acaaaatttg gtttgataat gcgcttaccg ataaagcact tgaaacaata cccaaacaaa
      841 cggaacatat aaatattgaa ctggcaaccg cttattgggg tcatcctcaa aatttacagt
      901 tagcgtatcg tataaaaggg tttagcaaaa tttggaaacc aatagatgcc gaacgaaaat
      961 ctatttactt ttacaaaccg cctgcaggca attatgtttt agaaatcaga aaatgtattg
     1021 gtaacaaagc caatgaattt gctttacttt ctataccgtt tagcgtagaa cagaaacttg
     1081 ttgagtcaaa tatttttatt gttgcaatat gccttgctat tttaactttc attttggtgg
     1141 tggtaaaatt agttactcaa aatattcaac gtaaaaatag actacttgaa aacaaagtat
     1201 tgcaacgtac aaatgaattg caagttgcaa acaaccagct aaaacaatcc attgtagcaa
     1261 aagataaact catttccatt gtatcgcacg aaattgtaac accgcttcgg tttttagcaa
     1321 tggtagccaa acaaaccggc tcgaaaccca tgtcagaaaa aaactggctg ccggcaatga
     1381 atgatattaa aaataccagc gaaagtttat ataacaatgc gcaaaacatt ctgaactgga
     1441 ttaaacatca gaacaagcgt tttaatgtta caataaacca tattgcttta agcggctttt
     1501 gtgatgaaat tactacggta tttaacgaaa tggcgtttgc taaaaacatt cattttaaaa
     1561 atgaaattgc agacgatacc attatcaaat cagataaaaa tattttagga ataattgtaa
     1621 acaacttatt tagcaatgct gtaaaattta ccgaaaatgg caccatcaca ataacttgcc
     1681 aaatgcaaac cgaaaacttt acgctcacaa ttaccgacac cggaaccggt ataaaccaac
     1741 aacaaatgaa tcggattcac ggtatcatcc aaaaaaacaa tacagaggtt tcgtttcact
     1801 ccgatagcag tggcaataat ggcttggggt atttaataat tgttgatttg ctgtcgcttt
     1861 taaatggcag catagctata agcagtaaat taaacgaagg aaccaaagta actttaacat
     1921 tacctataca gtaactgtta cagtgtgtat taattacgca actactttac ttctttaagc
     1981 attaataaat acgttggaaa atggtcgcta taacctgcta tgtaaatatt aaaatcgtag
     2041 gtgcgttttg cataaccttt atatcggcct gtttgctgca acataaactg ctt
//
LOCUS       DAOMAA010000352         2088 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_52087_length_2088_cov_3.159862, whole genome shotgun sequence.
ACCESSION   DAOMAA010000352 DAOMAA010000000
VERSION     DAOMAA010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52087_length_2088_cov_3.159862"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1106
                     /locus_tag="PLO59_05490"
     CDS             <1..1106
                     /locus_tag="PLO59_05490"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HQV00592.1"
                     /translation="FSIGFFLNVLAPIILAFVFKNDVDEIFSRYKIISYLPQIGLCFY
                     PLTVAFVLGVRNGNNQKQLVEQLVYIKKLGQENLLKESEKKKILEEQNIVLEHMVKQR
                     TQELALKNELIMVKNREITDNVTYAKRIQAAILPNRKTIQQHLPQSFILYIPKDIVSG
                     DFYGFSVKQNKIIIAAADCTGHGVAGAFMSMVGSSLLNQIINEKNITTPALILDELNE
                     GIIESLKQRDSVSNDGMDIAICAFDNDFKTVSYAGANRPLWLIRQGQLYVTIPNKFPI
                     GGMQVLHDEKFTNHTISLQPGDTLYIFSDGYADQFGETTGKKLMTKKFKEVLLHQQDQ
                     SMAQQKENLRQTLLSWQGNTEQVDDILVIGIRI"
     gene            1112..>2088
                     /locus_tag="PLO59_05495"
     CDS             1112..>2088
                     /locus_tag="PLO59_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00593.1"
                     /translation="MPFAKTLHAIKSFTAIVLLYLTYNNALATNVVNIRTGSQTKNKT
                     EIDLTNWKSRNADSAVFAGTNYNDSHWKLLNIEDLNNDDADGSVRWYRIHFIADSNMQ
                     NIPLAFFIRQFGSAAEFYLDGKLLKKSGVIGTSLQTEEAVLNLEPAHIIVVLNQKAHV
                     LAIRCSDFHRVKSSNNGISIDGFTDFRFEKIEGDISDLVHYKKYFPFIFFTGLFVALF
                     ALHLVMYIFNRDKTVNAFYSMYCLGVAFISLYTYYIITSTSFNLVNSLTQIAISVLPL
                     LVIPLVGLLHIIFYNKLGKLFITIICLYITLLLCMLFSGITAMVFTLILS"
BASE COUNT          700 a          369 c          368 g          651 t
ORIGIN      
        1 ggtttagtat tggtttcttt ttaaacgttt tagcacccat tattttggca tttgttttta
       61 aaaacgatgt agatgaaatt ttttctcgct ataaaataat cagctattta cctcaaatag
      121 gcttatgctt ttatccgcta acggtagcct ttgttttggg tgtacgtaat ggaaataatc
      181 aaaaacaatt ggtagaacaa ttggtttata ttaaaaagtt aggacaggaa aatcttttaa
      241 aagaatcgga aaagaaaaaa atactcgaag agcaaaacat tgttttagaa cacatggtaa
      301 aacaacgcac ccaagagttg gctttaaaaa atgaattaat catggttaag aaccgtgaaa
      361 ttaccgacaa tgtaacttat gcaaaacgca tacaagctgc catattacca aaccgcaaaa
      421 ccattcaaca acatttacca caatcgttta ttttatacat accaaaagat attgtaagtg
      481 gcgattttta tggtttctcg gtaaaacaaa acaaaattat tattgccgct gccgattgta
      541 caggccatgg tgtagccggt gcatttatga gcatggtagg cagctcctta ctaaaccaaa
      601 ttataaacga aaaaaatata accacacctg cattaatctt agacgaatta aacgaaggta
      661 ttatcgaatc attaaaacaa cgcgatagtg tttctaacga tggtatggat atagccattt
      721 gtgcttttga taacgatttt aaaacggttt cctatgccgg tgcaaaccgg ccactgtggc
      781 ttataaggca aggtcagttg tatgtaacca tacccaataa attcccaatt ggtgggatgc
      841 aggttttgca cgatgaaaaa tttaccaacc acactataag cctgcaaccg ggcgatacat
      901 tgtacatttt ttctgatgga tatgccgatc agtttggcga aacaaccggt aaaaaattaa
      961 tgactaaaaa gtttaaggaa gttttgcttc atcaacaaga ccaaagcatg gcacaacaaa
     1021 aggaaaacct acggcaaaca ttattaagct ggcaaggcaa tacggagcag gttgatgaca
     1081 tactggtaat tggtattaga atataaacaa catgccattt gcaaaaacat tacatgctat
     1141 aaaaagcttt acggctatcg tcttattata cttaacttat aataacgcat tggccactaa
     1201 tgtagtaaac atccgtacag gcagccaaac aaaaaataaa actgaaattg atttaaccaa
     1261 ttggaaaagc cgcaatgccg atagtgcagt ttttgccggc acaaactata atgacagcca
     1321 ttggaaacta ctaaatattg aagacctaaa caatgacgat gccgatggca gtgtacgttg
     1381 gtatcgcata cattttattg ccgatagcaa tatgcaaaat ataccacttg ccttttttat
     1441 taggcaattt ggatcggctg ctgaatttta tttagatgga aaactactaa aaaagtcagg
     1501 tgttattggt accagtttac aaactgaaga agctgtgtta aacctggaac ctgcacacat
     1561 tattgttgtg ctaaatcaaa aagcacatgt actggcaata cgttgcagcg attttcaccg
     1621 tgttaaaagc agtaataatg gaattagcat tgatggtttt accgatttcc gtttcgaaaa
     1681 aatagaagga gatataagcg atctagtcca ttataaaaaa tattttccgt ttattttttt
     1741 tacgggtttg tttgttgccc tttttgcgct tcacttggtg atgtacattt ttaatcgcga
     1801 taaaacggtt aatgcttttt acagtatgta ttgtttaggt gtagcgttta tttcgcttta
     1861 tacctattac atcattacct ccacaagttt taatctggta aacagtttaa cccaaattgc
     1921 catatcggtt ttgccccttt tggttatacc acttgtaggc ttactgcaca ttatttttta
     1981 caacaaatta ggcaaactgt ttatcactat tatctgcctc tacattacct tgctgttatg
     2041 catgttattt agtggtataa cggcaatggt atttaccctc attctatc
//
LOCUS       DAOMAA010000353         2087 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_52131_length_2087_cov_3.288878, whole genome shotgun sequence.
ACCESSION   DAOMAA010000353 DAOMAA010000000
VERSION     DAOMAA010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52131_length_2087_cov_3.288878"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1133
                     /locus_tag="PLO59_05500"
     CDS             <1..1133
                     /locus_tag="PLO59_05500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HQV00594.1"
                     /translation="ISFVGLIYSFLKHRTKNLENSKNALSQKVIERTRELNRTNLELS
                     KISLVASETSNAVLIFNSDLILEYANAGFTKMTGYQIDEFVKLRGNSIYKITYFEKIE
                     NLITEALRDRKSMQYESMITRKDGSKIWSATTLTPIFNSSGNLKNIVLIDTDIDSIKQ
                     IEQQLMQSFEERGLLLKEIHHRVKNNLQIIISLFNLQSNFIDDAVALKALREGQNRIK
                     SMALVHERFYQSEGLSKIDFDVYIHRLAETLFGSYNISSEKIKLNIEAEKISLDIDTA
                     IPCGLILNELINNSIKHAFNENQQGEIIVQFYSLNEQTCRLVIGDNGKGMDADFDFEN
                     AETLGMQLITALTQQIDGKLNIEHTNGLKFIIDFKKQFAELI"
     gene            1208..>2087
                     /locus_tag="PLO59_05505"
     CDS             1208..>2087
                     /locus_tag="PLO59_05505"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQV00595.1"
                     /translation="MRKTKIIVIEDEVIVAKDIQKSLIQLGYDVPAVANSAQVAFEKI
                     DEYEPDLVFCDIKLKGSIDGIDIASRLREEYLIPVIFLTSYVDKETLDRAKVTEPYGY
                     IVKPFNQTDLETTVAMALYKFEKDMEVRDNEKRFANVISNIDEAICIVNLEDKVTFLN
                     TRAEQLFKLSFANSLGHNLAQLLTISHNNYMHGVVKMPVTLNNATISNVHNLQPVSCN
                     IVISPIHDEINQLIGNAIIIRPNAFANESEVLAPPLSKESEEAMVFQDSFFVKKGSVL
                     VKVFLTTSFEGGRHKRR"
BASE COUNT          739 a          328 c          395 g          625 t
ORIGIN      
        1 caatcagttt tgttggctta atttatagtt tcttaaaaca tcgcaccaag aatctcgaaa
       61 acagtaaaaa tgcactaagt caaaaagtta tcgaacgtac ccgcgaactt aaccgtacta
      121 atctggagct atcaaaaata tcgttagttg ccagcgaaac aagtaatgcc gtactcatat
      181 ttaatagcga tttaatttta gaatatgcca atgcaggttt tactaaaatg accggatatc
      241 agattgatga atttgtaaaa ctaagaggca attccattta caagattacc tattttgaaa
      301 aaattgaaaa ccttataacc gaagccttac gggatagaaa gtcaatgcaa tatgaatcta
      361 tgattacccg caaggatgga agtaaaattt ggagtgccac cacattaacg cccatattta
      421 atagcagtgg taatttaaaa aatattgtac ttattgatac ggatattgat agcataaaac
      481 aaattgagca acaattaatg caatcgttcg aagaacgtgg tttgctgctt aaagaaattc
      541 atcaccgggt taaaaacaat ctgcagatta ttatcagcct gttcaatctg caatcaaatt
      601 ttattgatga tgcagttgca ttaaaagcat tacgcgaagg tcaaaaccgc ataaaatcaa
      661 tggctttggt acatgagcgg ttttaccaat cggaaggact ttcaaaaatt gatttcgatg
      721 tgtacataca tcgcctggcc gaaacccttt tcggttcata caatatcagt tccgagaaaa
      781 taaaattaaa tattgaggcc gaaaaaattt ctttggatat tgatacagct ataccttgcg
      841 gcctgatatt aaatgagctg ataaacaact ccataaaaca tgcatttaac gaaaaccagc
      901 aaggcgaaat tattgtgcag ttttatagtt tgaacgaaca aacatgccgc ttggttattg
      961 gcgataatgg taaaggaatg gatgctgatt ttgatttcga aaatgccgaa acgttaggta
     1021 tgcagttaat aactgcgcta acgcaacaaa ttgacggcaa attgaatatt gagcatacga
     1081 atggcttgaa atttataatt gattttaaaa aacaatttgc tgaattaatt taacaactgt
     1141 atttcatttt tatagatttg aaacgattgt tttaaaacga tataaggata aactaaaaaa
     1201 tcaggtaatg cgtaagacaa aaattattgt aatcgaagat gaagtgattg tagcaaagga
     1261 tattcagaaa tcacttattc aattgggcta tgatgtaccc gctgttgcta attccgctca
     1321 ggttgctttc gaaaaaattg acgaatacga acctgatttg gttttttgtg atataaagct
     1381 aaaaggcagt attgatggca ttgatatagc atctcgttta cgtgaagagt atctgatacc
     1441 ggttatattt ttaaccagtt atgttgacaa ggaaacactt gatagggcaa aagtaaccga
     1501 accttatggc tacatagtaa aaccatttaa tcaaaccgat ttggaaacta cagttgccat
     1561 ggcactctat aaatttgaaa aagatatgga ggtgcgcgat aatgaaaaac gttttgctaa
     1621 tgtgattagt aatattgatg aagcgatttg tattgtgaat ttagaagaca aagtaacatt
     1681 tttaaatacc agagctgagc agttgtttaa gttaagtttt gccaactctt taggacataa
     1741 tttggcgcaa ctactcacaa ttagccataa taattatatg cacggtgtgg taaaaatgcc
     1801 ggttacatta aataatgcaa ctataagcaa tgtccacaat ttacagccgg tttcgtgtaa
     1861 tatagttatc tcgcccatac atgacgagat aaaccagtta attggaaatg caattattat
     1921 caggcccaat gcatttgcaa atgagagtga agttttagcg ccaccgctat ctaaagagag
     1981 tgaagaggcc atggtgtttc aggatagttt ttttgtaaaa aaaggttcgg tgcttgtaaa
     2041 agtatttctt accacctctt ttgagggtgg caggcataag agacgca
//
LOCUS       DAOMAA010000354         2082 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_52406_length_2082_cov_2.270844, whole genome shotgun sequence.
ACCESSION   DAOMAA010000354 DAOMAA010000000
VERSION     DAOMAA010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52406_length_2082_cov_2.270844"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="PLO59_05510"
     CDS             complement(<1..743)
                     /locus_tag="PLO59_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HQV00596.1"
                     /translation="MYICHSYYSLKYGTISPRQLLEIGHNHGVQYMALADINTTSGIL
                     DFFRLAPQYNIVPIAAVDFRLQWPSGATLQFTAMAINAQGFFEINNYLTAYLTQQTEA
                     TALAPPFNHVFVFYPFSYTCLKPAQLRRHEFIAVSAADVNKLAFNAWQKHLHKLVAWP
                     AFTFRNKTDFNTHRLLRAIHYNTLLSKLDVQQQSNLQACFANPAAWQQTFTNYRLLLK
                     NMQQLMEQCYIDFEFGINKFHIILYDSTS"
     gene            complement(892..1611)
                     /locus_tag="PLO59_05515"
     CDS             complement(892..1611)
                     /locus_tag="PLO59_05515"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HQV00597.1"
                     /translation="MCYNISYMEKRAQRIAQHYAAAFSNDAQFEIQYQVSGFTHPLLP
                     VITMQQPQTIQAFTWGLIPYWCKQPQQAKEIAAQTLNAKCETIFEKPSYRKAIAGQRC
                     LLIVNGFYEWQTANKIKYPYYIQLKNSPFFSLGAVYDTWLNTETQSTHHTFSVVTVPA
                     NTLMQQIHNTMKRMPLILTPDTEKKWLQPNLDKPAIAQLMLPFDPNQMQAHTIARYNN
                     SHNHANIQQPYTYPELDLYNA"
     gene            complement(1627..>2082)
                     /locus_tag="PLO59_05520"
     CDS             complement(1627..>2082)
                     /locus_tag="PLO59_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00598.1"
                     /translation="ASQKNYGHLMLMQFKAPQQQQQAIIDAVQQVCALQTPIPIILQT
                     YMHHNKQNIYLPVQKVNTLTQLVKSLQAQLQLPHHQTYVAYNPCINVISGLTQNVFDT
                     VFQHFEHVNYNTTFIADTLMLLTRTSAYEPYTLIHEFDFNNMQTVPVIN"
BASE COUNT          560 a          323 c          517 g          682 t
ORIGIN      
        1 ttagaggtac tgtcatacaa gataatgtgg aatttattaa taccaaattc aaaatcaata
       61 tagcactgct ccataagttg ttgcatgttt ttaagcagta gcctgtaatt ggtaaaggtt
      121 tgctgccatg cagccggatt tgcaaagcaa gcctgtaagt tgctttgctg ctgcacatct
      181 aatttgctaa gcagtgtgtt gtaatgtatg gcgcgtaaca aacgatgtgt attaaaatcg
      241 gttttgttgc gaaatgtaaa tgccggccat gccactaatt tgtgaagatg tttttgccag
      301 gcgttaaatg caagtttgtt tacatcggct gcgctaacgg caataaattc atgcctgcgt
      361 aattgtgccg gttttaggca ggtgtagcta aacggataaa aaacaaatac gtgattaaag
      421 ggtggcgcca aagctgtagc ttcggtttgc tgagtgaggt aagcggttag gtaattgttt
      481 atttcgaaaa agccttgtgc attaatggcc attgccgtaa attgtagtgt tgcgcctgat
      541 ggccattgta atctaaaatc aactgcggca atgggcacta tgttgtattg tggtgccagt
      601 ctgaaaaaat cgagtatgcc cgatgtggtg ttgatatcgg ccagagccat atactgcaca
      661 ccgtggttat gccctatttc taacaattga cgtggactta tggtaccata ttttagacta
      721 taataactgt ggcatatata catggtagta aaacagataa tacattaacc cagattaaaa
      781 atgggaggta atgaaacgga gtgcagctta atgcaacaac tgtatttagt aaaacaattt
      841 gtaaaagttt atttgtgaag acgcttatca ggctaggtat ttaaatagcc gtcaggcatt
      901 atataaatcc agttcggggt aggtgtaagg ctgttgtata tttgcgtggt tgtggctgtt
      961 gttataacgg gctatggtat gtgcctgcat ttggttgggg tcaaagggca gcattagttg
     1021 cgcaatggcg ggtttgtcta aattaggttg cagccatttt ttttcggtat cgggtgtaag
     1081 tatcaggggc atgcgtttca tggtattgtg tatttgttgc atcagcgtat tggccggtac
     1141 ggtaacaaca gaaaaagtgt gatgtgtact ttgtgtttcg gtattaagcc aagtatcata
     1201 cacggcacct aatgaaaaaa acggactgtt tttaagttgt atgtagtaag ggtattttat
     1261 tttattggcg gtttgccatt cataaaatcc gtttacaata agcaggcaac gctggccggc
     1321 aatggccttt cggtacgatg gtttttcgaa aatggtttcg catttggcat taagtgtttg
     1381 tgctgctatt tctttggctt gctgtggctg tttgcaccag tacggtataa ggccccaggt
     1441 aaatgcctgt atggtttgtg gctgttgcat ggtaataaca ggcagtagcg gatgtgtaaa
     1501 accgcttacc tggtattgta tttcaaactg tgcatcatta ctaaatgcag cagcataatg
     1561 ttgggctatg cgctgggcgc gtttttccat gtaggatata ttgtaacaca tactatatat
     1621 gttttattaa tttattacag gtacggtttg catattatta aaatcaaact catgtataag
     1681 ggtgtaaggt tcgtaggccg atgtacgggt aagcagcatt aaggtatcgg caataaaggt
     1741 ggtattatag ttaacgtgtt caaaatgctg aaacacggta tcgaacacat tttgtgtaag
     1801 ccccgatata acattaatgc atgggttgta tgctacataa gtttggtgat gtggcaattg
     1861 taactgagct tgcagtgatt taaccaactg tgtaagtgta tttacttttt gcacaggcag
     1921 ataaatattt tgtttgttgt gatgcatgta tgtttgcagg ataatgggta tgggtgtttg
     1981 caaggcacac acttgttgca cagcatcaat aatggcttgt tgctgctgtt gtggtgcttt
     2041 aaattgcata agcattaggt ggccatagtt tttctgactg gc
//
LOCUS       DAOMAA010000355         2081 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_52432_length_2081_cov_3.840079, whole genome shotgun sequence.
ACCESSION   DAOMAA010000355 DAOMAA010000000
VERSION     DAOMAA010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52432_length_2081_cov_3.840079"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="PLO59_05525"
     CDS             complement(<1..216)
                     /locus_tag="PLO59_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00599.1"
                     /translation="MKKHILTFVAIILCNYIWAQCVPNTSIVNPGIYPDSATGLAGGF
                     VSTPYNEVIQIKVPLDTVAQTPLGQATV"
     gene            complement(282..1049)
                     /locus_tag="PLO59_05530"
     CDS             complement(282..1049)
                     /locus_tag="PLO59_05530"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HQV00600.1"
                     /translation="MQRFSFCVHDILQPQWQQAIPNFNEFDLQVWLIDTNNTALYNPL
                     VKLLNQDELERLSRFKQQDDKTRFVFGRGALRLLVGRYLNQKPETITFTESITKKPCI
                     EICFGNLFFNLSHSGSKILIGFSSHHEIGVDIEQIRPAFKIDDFMERNYAANEISDIA
                     KSAAPLSHFFKYWSRKEAWLKAVGIGVFGNLKAVDTTGLSPYIQTETFIAGAFADKFD
                     FYTFSVDDYWASVGINNNHTMPHFYSINIADLFETTS"
     gene            complement(1052..>2081)
                     /locus_tag="PLO59_05535"
     CDS             complement(1052..>2081)
                     /locus_tag="PLO59_05535"
                     /inference="COORDINATES: protein motif:HMM:NF013166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thioesterase domain-containing protein"
                     /protein_id="HQV00601.1"
                     /translation="FFELGGHSIIAVRLMIEIEYLKKIRLPLAVLFTNPTLVQLAQVI
                     DNEASGELWKSIIPIREGGNHAPVYFAHGVSGNVFKYHTLGKLLDNNTPSYGLQARGL
                     NGLDKPFSDLVEMAAYHTSEILKMQPDGPYFLGGGSFGGTLAYEIACQLSAMGKEIGF
                     VALFDVEAATKSEALEGVEKQIVELKLKSQRVVSRLKLLANQSMDDKLVYLKNKINNK
                     NPQVDIRKKQIDEWLEKELIAEKYGTVSADYFNNVEENCYKALKNYHINSSNFHCILF
                     RAKNGFYSPIEYENDLGWGYFAKGKLEVVSTPGNHNSIFDQPNVIELANQLMKQINAY
                     YANKKQLN"
BASE COUNT          610 a          389 c          371 g          711 t
ORIGIN      
        1 taccgtagcc tggcctaagg gtgtttgggc aaccgtatct aagggaactt taatttgaat
       61 tacctcgtta tagggcgtac ttacaaaacc acctgctagg cccgttgcac tatcggggta
      121 tatgcccggg tttacaattg aagtattggg cacacattgg gcccatatat agttgcacaa
      181 aattatggct acaaatgtta gtatgtgttt tttcatgcga gggcgatttt atggaagggt
      241 aattttagta aaaatttatc cggcaccaag atgtaaattt tttaggatgt tgtttcaaat
      301 aaatcggcaa tgtttatgga ataaaaatgt ggcatggtat gattgttatt tatccccacg
      361 ctagcccagt aatcatcaac tgaaaaggtg taaaaatcaa atttatcggc aaaggcacct
      421 gcaataaatg tttcggtttg tatgtatggg ctaaggcctg ttgtgtcaac tgcttttaaa
      481 ttaccaaaaa cgccaatacc cactgcctta agccaggctt ctttacgact ccaatactta
      541 aaaaaatgtg ataatggtgc tgcacttttt gcaatatcac ttatctcatt ggctgcataa
      601 tttctttcca taaagtcgtc aattttaaaa gccggacgaa tttgttctat atcaacacca
      661 atttcatgat gtgaactaaa tccaattaaa attttactgc ccgaatgcga tagattaaaa
      721 aataaattgc cgaaacaaat ttctatgcat ggttttttgg ttatggattc ggtaaaagta
      781 atcgtttcgg gtttttgatt gagatacctg ccaactaata accgcaatgc gccccgtccg
      841 aatacaaaac gcgttttgtc atcttgttgt ttaaaccggc ttaagcgttc caactcatct
      901 tgatttagca acttaactaa gggattatac agtgctgtat tattcgtatc aattaaccaa
      961 acctgtaaat caaattcatt aaaattaggt atggcttgtt gccattgtgg ctgtaaaata
     1021 tcatgtacac aaaatgaaaa ccgttgcatc tctagttaag ttgtttttta ttagcgtagt
     1081 atgcatttat ttgtttcatg agttggtttg caagttcaat tacattgggt tgatcgaata
     1141 tagagttgtg atttccgggt gtgcttacaa cttcaagttt accttttgca aagtagcccc
     1201 atcccaaatc attttcatat tcaattggcg agtaaaatcc gtttttggcc ctgaataaaa
     1261 tgcaatgaaa atttgaactg ttgatatgat agttttttaa ggctttgtaa cagttttctt
     1321 caacgttgtt aaagtaatcg gccgaaacgg taccatattt ttcagctatc aattcttttt
     1381 caagccattc gtctatttgc tttttacgaa tatcaacttg tggattttta ttgttgattt
     1441 tatttttcaa atacaccaac ttatcatcca tgctttgatt tgctaatagt tttaatcgtg
     1501 aaacaacacg ttgtgatttt aatttaagtt ctacaatttg cttttcaaca ccttctaatg
     1561 cctctgattt ggtggcggct tctacatcaa ataaagccac aaaaccaatt tcctttccca
     1621 ttgccgaaag ttggcaggca atttcatagg ctaacgttcc gccaaatgat ccaccgccta
     1681 aaaagtatgg tccatcgggt tgcattttta aaatttctga ggtatggtaa gcagccattt
     1741 ctactaaatc gctaaagggt ttgtctaaac catttaggcc acgtgcttgc aaaccataag
     1801 aaggtgtgtt attatctaac aatttgccta atgtgtggta cttaaataca ttgccgctta
     1861 caccatgtgc aaaatatacc ggtgcatgat tgccgccttc gcgtatgggt attatcgatt
     1921 tccaaagttc gcccgatgct tcattgtcaa ttacctgtgc caattgtact aatgttggat
     1981 ttgtaaacag cactgccaat ggcaatctta ttttttttaa atattcaatt tcaatcatta
     2041 accgaacagc aataatgcta tgcccgccta attcgaaaaa a
//
LOCUS       DAOMAA010000356         2080 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_52484_length_2080_cov_2.887901, whole genome shotgun sequence.
ACCESSION   DAOMAA010000356 DAOMAA010000000
VERSION     DAOMAA010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52484_length_2080_cov_2.887901"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            186..1757
                     /locus_tag="PLO59_05540"
     CDS             186..1757
                     /locus_tag="PLO59_05540"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV00602.1"
                     /translation="MIWQTKLFAQAQQFTVAVTDTATLLSMPAVHSGAYAVYDGCWFV
                     IGGRINGLHGFTPPNGFKPNGINSMVYMISPANDTRKEKHVMQDISDTAMLDALQAAF
                     YQFAQNDSMLYITGGYGLLTDSADNYTLPNLIAVNLKQLKTALNNNTSIAPSFRLITD
                     PLLKVCGGNMYRTDSTYHLVFGHDFNRSYSVNDTLGFFTQQYTNAIRSFNINDDGISL
                     SISNISIITDSVNFHRRDYNLVPQIFPDGTTGQTAFSGVFQYYTRQPYLNCVNIKPTG
                     YDVINNFNQNLSQYQSACMPVYNAIDNKMSTVFFGGMSLYTYDTTTQTFLVDSMIPFV
                     PTISKVVRHADSSLTEYLNPTEMPSLIGTNSIFIIDDSIPTYTNGIINLNALPYGTTR
                     VGSIIGGINSDLPHVSNTPNFSYASGFVMRVYLTKIIDTKTPETEVKNNIINLIAYPN
                     PTTKNITIQFELNKPEQVQIAILNATGSRIKTMELPLQPSGFYQINYQAEKQGLYYSK
                     VITPTFTKTVTFIVK"
     gene            1949..>2080
                     /locus_tag="PLO59_05545"
     CDS             1949..>2080
                     /locus_tag="PLO59_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017259932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HQV00603.1"
                     /translation="MASIKQKFQMEFEMRSSPKILYNYISTASGLSEWFADDVRVKEG
                     "
BASE COUNT          712 a          373 c          349 g          646 t
ORIGIN      
        1 gctgttttta tatgaaacgg agtagataaa aaattaatgg gggttaataa aatctactcc
       61 gttttagctg gtttagttac aactaaaact atttatatta ttaatgttat tggctaacaa
      121 tactaaagct acatttgaac ctttggtatc caataaacat gttcaaatat ttaccgattt
      181 tacttttgat ttggcaaaca aaattgtttg cgcaagcaca acagtttacc gttgccgtaa
      241 ccgatacagc aaccttactt agtatgcctg ccgtacactc gggtgcttac gcagtttatg
      301 atggatgctg gtttgtaatt ggggggcgca taaacggatt gcatggcttt acaccaccaa
      361 atggatttaa acctaatggt ataaacagta tggtttacat gattagcccg gccaatgata
      421 cccgtaaaga aaagcatgta atgcaagata tatccgacac agctatgctt gatgcattgc
      481 aggcagcatt ttatcagttt gcacaaaatg acagtatgct ttatataaca ggtggttatg
      541 gtttactaac tgatagtgcc gataactata ccttaccaaa cttaatagct gttaatttaa
      601 agcaacttaa aactgcatta aataataaca cttctattgc accaagtttt agattaataa
      661 ccgacccatt acttaaagtt tgtgggggta atatgtatag aaccgactca acttaccatt
      721 tggtttttgg gcatgatttt aacaggagct attcggtaaa cgatacgttg gggtttttta
      781 cacagcaata caccaatgct atacgcagtt ttaatattaa tgacgatggc attagcttat
      841 caataagcaa cataagtatt ataaccgact cagttaattt tcacagacgc gattacaatt
      901 tagttccgca aatatttcct gatggcacaa cagggcaaac agcatttagt ggcgtttttc
      961 aatattacac ccgacagccg tatttaaatt gtgtaaacat taaacccaca ggctacgatg
     1021 taataaataa ttttaatcaa aacctgtcgc aatatcaatc ggcttgtatg cctgtgtata
     1081 atgcaattga taataaaatg agcaccgtat tttttggtgg catgagttta tatacatacg
     1141 atacaacaac acaaacattt ttggttgata gtatgatacc gtttgtacct acaataagta
     1201 aagtagtgcg ccatgctgat agctcgttaa ccgaatattt aaacccaact gaaatgccaa
     1261 gcttaattgg caccaacagc atttttataa ttgatgatag cataccaact tatacaaatg
     1321 gcattattaa tttaaatgcg cttccctatg gcaccacacg tgtaggaagc attattggtg
     1381 gcattaacag tgatttgcca cacgttagca acacgcctaa tttttcgtat gcatcaggtt
     1441 ttgttatgcg tgtttacctt acaaaaatta ttgacacaaa aacacctgaa accgaagtaa
     1501 aaaacaatat cataaacctg attgcatatc caaacccaac cacaaaaaac ataaccattc
     1561 agtttgaact taataaacct gagcaagtgc aaatcgccat tttaaatgca actggcagtc
     1621 gaataaaaac catggaactt ccattacaac cttccggatt ttatcaaata aattaccaag
     1681 cagaaaaaca gggcttgtac tatagtaaag ttataacacc aacttttacc aagactgtaa
     1741 cttttatagt taaataaatt tagcccacca ccttaaaagt aagaatttga tgctacagcc
     1801 tatgatttag gttttgtttt ttgcatgttt atttcaccga aaaattttta ggttgaaaat
     1861 tattttgcat gtttaacccg aagccaattg cagtaatgaa agttgaacaa acggtttttt
     1921 aaaaacaatc taatttaggt caattaagat ggcaagcatt aaacaaaaat ttcagatgga
     1981 gtttgaaatg cgttcttctc caaaaatatt atacaactat ataagtacag ccagtggttt
     2041 atccgagtgg tttgccgatg atgtacgtgt taaagaaggt
//
LOCUS       DAOMAA010000357         2079 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_52516_length_2079_cov_3.785573, whole genome shotgun sequence.
ACCESSION   DAOMAA010000357 DAOMAA010000000
VERSION     DAOMAA010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52516_length_2079_cov_3.785573"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="PLO59_05550"
     CDS             <1..583
                     /locus_tag="PLO59_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HQV00604.1"
                     /translation="SNVFIRSLATRGALGGLSAKTFEVVHVLQAAQYDYIFIETVGVG
                     QSEIDIASLAHTTVVVWVPESGDDVQMIKSGIVEIADVFVVNKADRDGAAIMAKNITS
                     ALHERKPLPWQIPVIKTIATKNEGVAQLIDAIHKHQSYTKTSLSNLQAQQLYNLIQLH
                     KMSGITMDALQQFINQNKQQNIYALALQYLSKQ"
     gene            627..1904
                     /gene="purD"
                     /locus_tag="PLO59_05555"
     CDS             627..1904
                     /gene="purD"
                     /locus_tag="PLO59_05555"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018943499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HQV00605.1"
                     /translation="MNVLLLGSGGREHAFAYKIAQSKMLTQLFVAPGNAGTLQCGTNV
                     NISVTDFETIHRFVIDNQIKLVVVGPEDPLVSGIADYFLQTPALQHVPVVGPVKAGAR
                     LEGSKEYSKKFMIKYGIPTAAYQSFNASNLELGYKFLESLLPPYVLKADGLAAGKGVL
                     ILNDLSEAKQMLQQMIMDKKFGTASTTVVIEEFMHGIECSVFVLTDGISYKILPTAKD
                     YKRIGDNDTGPNTGGMGAVSPVPFCDDAFMQKVTQQIIEPTFAGLKNEAINYCGFLFI
                     GLMNDGGNPRVVEYNVRMGDPETEVVLPRIQNDLLEMLWAAGTKNLEKTDLQISPKTA
                     VTVVMVSGGYPDAYQKNKPISGLDNVTESIVFHAGTTSSHDTVVTNGGRVLVITSMDN
                     DIAGAAAKSYKTISMIDFENQYYRTDIGNDLVP"
BASE COUNT          694 a          379 c          428 g          578 t
ORIGIN      
        1 cagtaatgtt tttataaggt cgttagccac acgtggcgct ttaggtggcc taagcgccaa
       61 aacttttgaa gtggtacatg tgttacaagc tgcacaatac gattatattt ttattgaaac
      121 cgttggcgtt ggacaaagcg aaatagatat tgcatcatta gcgcacacta ccgtagttgt
      181 atgggtgccc gaaagtggcg atgatgtgca gatgattaaa agcggaattg ttgaaattgc
      241 cgatgtgttt gtagtaaata aagccgacag agacggtgcc gctatcatgg ctaaaaatat
      301 aacatcggca ttacacgaac gcaaaccatt gccttggcag attcccgtaa ttaaaactat
      361 tgcaactaaa aacgaggggg ttgcccaact tattgatgca attcataagc atcaatccta
      421 tacaaaaacc agcttatcaa atttgcaagc acagcaattg tataacctca tacaactaca
      481 taaaatgagt ggtataacaa tggatgcatt gcaacaattt ataaatcaga acaaacaaca
      541 aaatatatat gcacttgcat tgcaatattt aagcaagcaa taaggctttt atgtttttat
      601 ttgccgcctt ataaacgaat tatataatga atgttttact attaggttca ggcggacgcg
      661 agcacgcttt tgcatataaa atagcgcaaa gcaaaatgct tacacaattg tttgttgcac
      721 cgggtaatgc cggcacatta caatgtggca caaacgtaaa catatcggtt accgactttg
      781 aaaccattca tagatttgtg atagacaacc aaataaaatt ggtagtagta gggcccgaag
      841 atcctttggt atcaggcatt gccgattatt ttttgcaaac acctgcacta caacacgtgc
      901 ctgttgttgg gccggttaaa gcaggggccc ggttagaagg cagcaaagaa tattcgaaaa
      961 aatttatgat caaatatggt attccaactg ctgcatatca aagttttaat gcaagtaatt
     1021 tagagttagg ttataagttc ttagaatcac ttttaccacc ttatgtttta aaggccgatg
     1081 gtttggcagc aggtaaaggc gttttaattt taaatgattt atctgaggct aagcaaatgt
     1141 tgcaacaaat gataatggat aaaaagtttg gaacagccag cactacggtg gtaattgaag
     1201 agtttatgca tggcatcgaa tgttcggttt ttgtgctaac cgatggcatc agttataaaa
     1261 tattgccaac ggctaaagac tacaaacgca ttggagataa cgataccgga ccaaatacag
     1321 gtggtatggg tgcagttagt ccggtgccgt tttgtgatga tgcctttatg caaaaagtaa
     1381 cgcagcaaat tatcgaacct acctttgccg gtcttaaaaa cgaagcgatt aattattgcg
     1441 gtttcttatt tatagggtta atgaatgatg gtggcaatcc gcgtgtggtt gagtacaatg
     1501 tgcgaatggg cgaccccgaa accgaagtgg ttttgccccg tatccaaaat gatttattgg
     1561 aaatgttatg ggcagcaggc acaaaaaact tagaaaaaac tgacttgcaa ataagcccta
     1621 aaaccgctgt aaccgttgtt atggttagtg gcggatatcc tgatgcgtat caaaaaaaca
     1681 agcccatctc aggcttagat aatgttacag agagcattgt tttccatgcc ggaactacca
     1741 gtagtcatga tacagtagtt acaaatggtg gacgcgtatt ggtaataact tcaatggata
     1801 atgatattgc tggggccgcg gcaaaaagct ataaaaccat cagcatgatt gattttgaaa
     1861 atcaatatta ccgtacggat attgggaatg atttagtacc ctgaaaaaca aaaaaagcga
     1921 agtcaaatct tcgcttccaa taattcttaa aatggttact tactttttgt cttcagcatc
     1981 gccacgaaca gctaaataat tatcgccacc cttttctaaa cgcataccat aaaacaacca
     2041 gtaaaaacaa ccaatggcaa ttaaacacca aaatataac
//
LOCUS       DAOMAA010000358         2073 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_52842_length_2073_cov_2.488603, whole genome shotgun sequence.
ACCESSION   DAOMAA010000358 DAOMAA010000000
VERSION     DAOMAA010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52842_length_2073_cov_2.488603"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..164)
                     /locus_tag="PLO59_05560"
     CDS             complement(<1..164)
                     /locus_tag="PLO59_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009294582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MscL family protein"
                     /protein_id="HQV00606.1"
                     /translation="MSFIKEFKEFAMRGNLIDFAVGVIVGGAFGKVTSSFVDGMVMPI
                     IGKMVGGMNF"
     gene            complement(265..972)
                     /locus_tag="PLO59_05565"
     CDS             complement(265..972)
                     /locus_tag="PLO59_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020080282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HQV00607.1"
                     /translation="MNSDDKKIRRAFTKKDWQEIKSDNSWSVFKIMAEFVEGFEKIGR
                     LGPCVSIFGSARVTDDDPYYKLAEEIAYQLTANGYGIVTGGGPGIMEAGNKGAQRGRG
                     RSVGLNIKLPFEQFVNPYVDADKDITFDYFFVRKTMFIKYSQGFIAMPGGFGTMDELF
                     ESLTLIQTKKIAKFPIILVGTEYWAGLVDWIKDTLIKGNYISVEDLDLFTLVDTPEEV
                     VDVINKFYSKYTLKPNF"
     gene            1045..>2073
                     /locus_tag="PLO59_05570"
     CDS             1045..>2073
                     /locus_tag="PLO59_05570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HQV00608.1"
                     /translation="MSYLIKGSGFLIIILLCVFFYHGNINEFPQHIHGWSQSDRYALA
                     IGFLNNGFDFFHPQTFNLNVQFEPADGLKKFTGITAVDFPIHEYLVAILMQCFGVSPW
                     VFRCYTLLYALVGLYFLARICKQLGLDNLWALFMPVFVLTTPVFTYYANGFLPGITAW
                     ANASIGFYFYVLCLQHRQKKHWILALVFFTLAALTRTPFIMCLLAIGLVQLIYNFKSG
                     HYQRMFWFYFIAAIAVIVGYTFYNNYLRKVYGSIFLSTPMPARNFAEFKQIAADIYKQ
                     WLFHYFSLAHYLVWLTFVFTLTVTIFKTKLNGWLGQFAYVQIVLLGGASCYALLMWRQ
                     MQQHDYYFI"
BASE COUNT          595 a          369 c          353 g          756 t
ORIGIN      
        1 ctaaagttca tacctcctac cattttaccg ataatgggca ttaccatgcc atcaacaaat
       61 gaagaggtaa ctttaccaaa tgcgccacct acaattacac ctacggcaaa gtcaattaaa
      121 ttgccacgca tggcaaactc tttaaattct tttataaaac tcattttgat tttgtttgaa
      181 gatttgtgaa ttgataactg attattttac ttatagtgca aataaaccgt tttttaatta
      241 tcaaacagcc ctgtattatg catattaaaa attgggttta agtgtgtatt tactataaaa
      301 tttattaatc acatctacca cttcttcggg cgtatcaact aatgtaaaaa gatctaaatc
      361 ttcaacagaa atatagttac cttttatcaa agtatctttt atccagtcaa ccaggcctgc
      421 ccaatattca gtacccacca atataatagg gaatttggca attttttttg tttgaatgag
      481 tgtaagcgat tcaaacaatt cgtccatagt tccaaaacca ccgggcatgg caataaagcc
      541 ttgcgagtat ttaataaaca tggttttgcg cacaaaaaaa taatcaaagg ttatatcctt
      601 atcagcatca acataaggat ttacaaactg ttcgaatggc agtttaatgt ttaagcctac
      661 cgagcgaccg cggccgcgct gcgcaccttt attaccggct tccattatac ctgggccgcc
      721 accggttaca atgccgtaac catttgcagt aagttgataa gcaatttctt cggctaattt
      781 ataataaggg tcatcatcgg taacacgggc cgagccaaat atgcttacac aagggcctaa
      841 gcgaccaatt ttttcgaatc cttcaacaaa ttcggccata attttaaata cactccagct
      901 attgtccgat ttaatttctt gccagtcttt tttagtaaag gcacgtctta tttttttatc
      961 atcactattc atacaaattg atttttaaat gatttacaat aatggtcttt ttcttttact
     1021 tggttattaa ttttatttgc atccatgtcg tacttaatta aaggcagcgg gtttttgata
     1081 attattttgc tgtgtgtttt tttctatcat ggaaatataa atgagtttcc gcaacacatt
     1141 catggttggt ctcaaagcga taggtatgcg ttagccattg gttttttaaa taatggattt
     1201 gatttttttc atccacaaac ttttaattta aacgtacagt ttgagcctgc tgatggttta
     1261 aaaaagttta ccggtataac agccgttgat tttccaatac atgaatattt agttgctatt
     1321 ttgatgcagt gttttggcgt ttcaccctgg gttttcaggt gttatacatt gctttatgct
     1381 ttagttggat tgtatttttt agcacgtatt tgtaagcagt taggattgga taatttatgg
     1441 gctttgttta tgcctgtttt tgtattaact actccggttt ttacctatta tgcaaatggc
     1501 tttttgcccg gtattactgc ctgggctaat gccagcattg gtttttattt ttatgtgtta
     1561 tgcctgcaac accggcaaaa aaaacactgg atacttgcat tggttttctt taccctggct
     1621 gcgcttacac gtacaccttt tataatgtgt ttgcttgcaa ttggcttggt acaactgatt
     1681 tataatttta aaagcggcca ttaccaacgt atgttttggt tttattttat agcagcaata
     1741 gcagtaattg ttggctatac tttttataac aattatttgc gcaaagtgta tggttccata
     1801 tttttaagca caccaatgcc agcacgtaat tttgcagaat ttaagcaaat agctgccgat
     1861 atatacaagc aatggttgtt tcattatttc agtttggcgc attatttagt ttggctgaca
     1921 tttgtgttta cactaacagt tacaattttt aaaacaaaac taaatgggtg gcttggacaa
     1981 tttgcatacg tgcaaatagt tttattaggt ggcgcaagtt gctatgcttt gcttatgtgg
     2041 aggcaaatgc aacaacatga ttattatttt att
//
LOCUS       DAOMAA010000359         2072 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_52887_length_2072_cov_2.443233, whole genome shotgun sequence.
ACCESSION   DAOMAA010000359 DAOMAA010000000
VERSION     DAOMAA010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52887_length_2072_cov_2.443233"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..>2072)
                     /locus_tag="PLO59_05575"
     CDS             complement(26..>2072)
                     /locus_tag="PLO59_05575"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HQV00609.1"
                     /translation="DALNMKGVASHYKVGALEVLTLKAGNDVLLCPENVPLAIDSIMN
                     AMQSGFLDSIDVYESVIKILQLKHWSGVLQTAIIDTNNLYQKLNANPYALNLNHQMYG
                     SAITLLRNRNNVLPLTSFSDSSLASIVINDSINNVFQMVLARYAISKNFKADKEISDE
                     RINNLVKTLSGYRKVILSIHNTTTKANANYGITPSTIKLIEKLAGNTEIILVVFGNTY
                     TLSKLQISSNVAAIVMAYEDTYLPNEIAAQVLFGAQQPTGSLPVSADSIFSIGNGLGR
                     IDKSNVLAYAPPLAVGANATVLAGIDSLALSAIENKIMPGCQVLVAYKGTVIYDKSFG
                     NKTYKEIAPVNNNMLYDVASITKVTATALATMYLVDKGLLDITKKVSKYLPEFKKSNK
                     RDLIIADVLTHQAGLMPFIPFYKETIEGNQYKSGVYSRVKTDAYSVQVADSFFVSETF
                     SKSLWQRIVQSELRDTGKYVYSDLGPIIMQHLIEKVAGTTLDSLVYDVFYKPMGLSRI
                     TFNPLQKFSKNEIVPTEFDTIFRKQLVHGFVHDQSAALLGGVSGNAGLFSNAESIAIL
                     MSMLMQQGNYGGHRYIDAATVQLFTGKYYTNNRRGLLFDKPDVDVIKSPCAKDASAST
                     FGHQGFTGTCAWADPTNELVFVFLSNRVHPLAENNALAKQNIRTRMMQIAYEAIGSK"
BASE COUNT          638 a          410 c          344 g          680 t
ORIGIN      
        1 atgttagctt agactaagac gctacttact tactgccaat tgcttcgtat gcaatttgca
       61 tcatacgtgt tcgaatgttt tgtttggcta aagcattgtt ttcggcaagt ggatgaactc
      121 tgttgcttaa aaaaacaaaa accaactcat tggttggatc tgcccaggca caggtgccgg
      181 taaatccctg atgaccaaaa gttgaagccg aagcatcttt agcacatggg cttttaatta
      241 catctacatc cggtttgtca aataataaac cgcgcctgtt attagtataa tatttaccgg
      301 taaacaattg aaccgtggcc gcatcaatat atcggtggcc gccataattg ccttgttgca
      361 taagcatgct cattaatatg gctatactct ctgcattcga aaataagccg gcattaccgc
      421 ttacaccacc taacaatgct gcagattggt catgcacgaa accatgcacc aattgctttc
      481 tgaaaatggt atcgaactcg gtaggaacta tttcattttt cgaaaatttt tgcaatgggt
      541 taaaagttat ccggcttaag cccatgggtt tataaaaaac atcataaaca agtgaatcaa
      601 gcgtagtgcc tgccactttc tcaataaggt gttgcattat tatcggacct aaatctgaat
      661 aaacatattt gcctgtatct cttaattcgc tttgtacaat acgctgccat aatgatttcg
      721 aaaatgtttc cgaaacaaaa aagctatcgg ctacttgtac actataagca tccgttttaa
      781 cccggctata aacacctgat ttatattgat tgccttcaat ggtttcttta taaaaaggaa
      841 taaatggcat taaacccgcc tgatgcgtta acacatcagc aattatcaaa tcacgcttat
      901 tacttttctt aaactcgggc aggtattttg atactttttt agttatatct aaaagtccct
      961 tatcaaccaa atacatagtt gctaaagcag tggctgtaac cttggttatt gatgccacat
     1021 catacaacat gttattgtta accggtgcta tctctttata agttttattt ccgaacgatt
     1081 tatcataaat aacggttccc ttatacgcaa ctaatacctg gcagcccggc ataattttat
     1141 tttcgattgc tgaaagtgct aaactgtcaa taccagccaa tacagtagca ttagcaccaa
     1201 ctgcaagcgg aggtgcatag gctaatacat tgcttttatc aatccttcct aacccattcc
     1261 caatactaaa aatactatca gccgaaaccg gtaacgaacc tgtgggctgt tgtgctccaa
     1321 ataatacttg tgctgcaatt tcattgggta aataggtgtc ctcataagcc attacaatag
     1381 cagcaacatt actgctaatt tgaagtttac ttaaagtata agtattacca aatactacca
     1441 aaataatctc ggtattgcct gcaagttttt cgattaattt tatggttgat ggcgtaatgc
     1501 catagtttgc attggctttg gtagttgtat tatgtatgct tagaattact tttctatatc
     1561 cggataatgt ttttacaaga ttattaattc tttcatcgct aatttctttg tctgctttaa
     1621 agttttttga aattgcatag cgtgctaata ccatctgaaa aacattgttt atcgaatcat
     1681 ttataacaat tgaagctaag ctgctgtcgc taaatgacgt aagtggtaaa acgttattac
     1741 ggtttcgtaa caatgtaatt gccgagccat acatttgatg attaaggttt aatgcatagg
     1801 gatttgcatt tagcttttga tataaattat tggtgtcgat aattgcagtt tgtaaaacac
     1861 cactccaatg ttttagttgc aatattttaa ttacactttc ataaacatca atactatcta
     1921 aaaaacctga ctgcattgca ttcataatac tatcaatagc aagcggtaca ttttcaggac
     1981 ataacaaaac atcgttgccg gcttttaaag taagcacctc caatgcacca actttataat
     2041 gcgatgcaac gcccttcata tttaaagcat cg
//
LOCUS       DAOMAA010000360         2071 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_52909_length_2071_cov_3.518353, whole genome shotgun sequence.
ACCESSION   DAOMAA010000360 DAOMAA010000000
VERSION     DAOMAA010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52909_length_2071_cov_3.518353"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="PLO59_05580"
     CDS             complement(<1..202)
                     /locus_tag="PLO59_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008759852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00610.1"
                     /translation="MNGQYISKQIDAQLFTFSDGLPSNIIYKTVVDSKGFLWLATHEG
                     LSRFDGQKFYNYYEDISKPNALQ"
     gene            424..1962
                     /locus_tag="PLO59_05585"
     CDS             424..1962
                     /locus_tag="PLO59_05585"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HQV00611.1"
                     /translation="MTNKLDQLQQNIAQAKLGGGQKRIDSQHSKGKLTARERLHLLFD
                     DGVFEEIGMLVTHRSVEFGLDREKYLGDGVITAYGNINGRLTYVFAQDFTVFGGSLSE
                     AHAGKICRLMDLAMQNGAPVVGLNDSGGARIQEGVVSLGGYADIFYRNTLASGVVPQI
                     SAIMGPCAGGAVYSPAITDFILMVENTSYMFVTGPNVVKTVTHEEVTSEELGGASAHS
                     IKSGVTHFSCANEIACIADVKRLLSYMPQNCEDTAPAVAYTAGNELRPALNNIIPPNA
                     NQPYDMREIIAQVVDNDFFEVHKNYAENIVVGFAHLAGQSIGIVANQPAVLAGVLDIH
                     SSKKAARFVRFCDCFNIPLLVFEDVPGFLPGTDQEWNGIISNGAKLLFAFSEATVPRI
                     TVITRKAYGGAYDVMNSKHIGADMNFAWPTAEIAVMGAAGAAEIIFKNEIAASADKSA
                     KLKEKELEYTQHFANPYRAAERGYVDEVIMPDQTRQKLIAAFNMLKNKVAKLPRKKHA
                     NIPL"
BASE COUNT          649 a          395 c          435 g          592 t
ORIGIN      
        1 tttgtaatgc atttggttta cttatatctt cataataatt ataaaatttt tggccatcaa
       61 aacggcttaa accttcatgt gtggcaagcc ataaaaagcc cttactatca accaccgttt
      121 tgtaaatgat attgctgggc aaaccatcgc taaatgtaaa caactgcgca tctatttgct
      181 tcgaaatata ttgcccgttc acatttgaaa aaaacaaaag gcaaaacact aaaaacaaaa
      241 agctatttct tactggtaac attatggaat aaaatagtta ctaaataatg tgttttattt
      301 tcagtaatta aatagttggg ctcaattttg ttttacatgc aaagtcaaca attttttaca
      361 gatgttaggc agtaacgcta tttaagtatc attgcaaccc tattagcaaa tgatatttaa
      421 accatgacaa ataagttaga tcaactgcag caaaatattg cacaagcaaa attaggagga
      481 ggccaaaagc gaattgattc gcaacacagc aaaggcaaat taacagcgcg cgagcggtta
      541 catttattgt ttgatgatgg cgtgtttgaa gaaattggta tgttggtaac ccatcgttcg
      601 gttgagtttg gtttagatag agagaaatat ttaggcgatg gcgttattac cgcttatggc
      661 aacattaatg gtcgcctgac ttatgttttt gcacaggatt ttacggtatt tggcggctcg
      721 cttagcgaag cacatgccgg taaaatttgc aggcttatgg atttggccat gcaaaatggt
      781 gcaccggtag ttggcttaaa cgatagtggc ggtgcacgca tacaggaagg tgtggtaagc
      841 ttgggcggct atgccgatat tttttatcgc aatacattgg catcgggcgt agtgccacaa
      901 ataagcgcca ttatgggccc atgtgccggt ggcgcagttt attcgcctgc cattaccgat
      961 tttattttga tggtggaaaa caccagctat atgtttgtta ccggccccaa cgtagtaaaa
     1021 accgtaacac atgaagaagt aaccagcgaa gagttaggag gtgcatcggc acacagcatt
     1081 aaaagtggtg taacacattt ttcatgtgct aacgaaattg catgtattgc cgatgtaaaa
     1141 cgcttattaa gctacatgcc gcaaaattgt gaagatacag cccccgcagt tgcttacaca
     1201 gcgggtaatg aattgcgccc tgcattaaac aatataatcc cacctaacgc caatcaacca
     1261 tacgacatgc gcgaaataat tgcacaagtg gttgataacg atttttttga agtacataaa
     1321 aactatgctg aaaatatagt ggtaggtttt gcacacctag ccggccaaag tattggtatt
     1381 gttgctaatc agccggctgt attagcgggt gtgctggata ttcactcatc aaaaaaagca
     1441 gcacgttttg ttcgcttttg cgattgtttc aacataccct tattggtgtt tgaagatgta
     1501 cccggttttt tacccggcac cgaccaggag tggaatggca ttataagtaa tggtgccaaa
     1561 ctgttatttg catttagcga agcaaccgta ccacgtatta cggttattac ccgcaaggct
     1621 tatggcggtg cttatgatgt aatgaacagc aagcacattg gtgccgatat gaactttgca
     1681 tggcccactg ccgaaattgc tgtaatgggt gcagccggtg cagccgaaat aatttttaaa
     1741 aacgaaatag ctgcttctgc tgataaatca gccaaattaa aagagaagga acttgaatac
     1801 acgcaacact ttgctaatcc ataccgcgct gccgaacgtg gttatgttga cgaagttata
     1861 atgcccgatc aaacacgtca gaaattaatt gctgcattca acatgcttaa aaacaaagta
     1921 gccaaattac ctcgtaaaaa gcatgctaac attccgttgt aatttattgc agtgtaattt
     1981 aggttacaaa ataattgtct gccaatgcat catcagctac gaaatcgttt gcacgctcat
     2041 gaaaagaatg acgaaaaact acatgttgca t
//
LOCUS       DAOMAA010000361         2052 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_53816_length_2052_cov_3.054081, whole genome shotgun sequence.
ACCESSION   DAOMAA010000361 DAOMAA010000000
VERSION     DAOMAA010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53816_length_2052_cov_3.054081"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1554
                     /locus_tag="PLO59_05590"
     CDS             <1..1554
                     /locus_tag="PLO59_05590"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQV00612.1"
                     /translation="DAVTVGKNESGNILISNTNNKTSASVDASNFTMPVLYKHAFDKK
                     GRTISFNLTPGFNSNKGNSNLYSYNVYALDSLSTDSINQLTNSDAQGKVFSGNVTYTE
                     PVGKKGQLLFTYNNNYNLNNSDKKTYNYQTVSDSYTDLDATLSNVFESRYMANNGGIS
                     YMYTQTKWNATAGLSYQVATLKNEQTFPQSDNLNKTFYSLLPNAQFQYKFTTSKNMRV
                     NYRSSNNAPSVTQLQNVVNNSNPLQLTAGNPNLSQDWQNNVNIRYSSVNTLKSTNLFA
                     VLNLTKTEDYIANTTYFALTDTQVAGVNMPAGSQLTVPQNLNGYYNVRMFSNYGFPFK
                     AIKSNLNINANANLNRTPGQINFVNNYAYTTSVGTGFSINSNISPKIDFSVGSYFSYN
                     NITNTQQSQANNEYLNLNTRLRMQFTIWKGLVLQSDVTNQHYAGLADNLNQDFILWNA
                     GIGYKFLKNDAAELRLTVFDILNQNNSFNRNTTETYFEDVTTNVLTQYFMINFTYNIK
                     YFKNAGKEN"
     gene            1642..>2052
                     /locus_tag="PLO59_05595"
     CDS             1642..>2052
                     /locus_tag="PLO59_05595"
                     /inference="COORDINATES: protein motif:HMM:NF016087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein NlpE N-terminal
                     domain-containing protein"
                     /protein_id="HQV00613.1"
                     /translation="MKIHRFIILIFALSFQFNCTTAQSENTYTQTISLSDNKQNLRLT
                     LGSDKYYRLIVQYADTPKMPEAYAGNYIRTKNVVALTGNVPYKYFSLNDNTMQWCDET
                     GRALNNQTVLTNDTGMHTSLDKRDEQKIKNGIDFF"
BASE COUNT          746 a          400 c          321 g          585 t
ORIGIN      
        1 gatgctgtta ccgttgggaa aaacgaaagt ggcaacatac taataagcaa cacaaacaat
       61 aaaacttcgg catcggttga tgcaagcaac tttaccatgc cggtattgta caaacatgca
      121 ttcgataaaa aaggccgcac catatctttt aaccttacac cgggcttcaa ctcaaataaa
      181 ggcaatagca atttatactc atacaacgta tatgcactcg actcgttaag taccgatagt
      241 ataaatcagt taaccaacag cgatgcgcaa ggcaaagtat ttagtggtaa tgttacctac
      301 accgagcctg ttggcaaaaa aggtcaatta ctttttacct acaacaacaa ttacaattta
      361 aacaacagcg ataaaaaaac atacaattac caaacagtat ccgattcata tacagattta
      421 gatgccactt taagtaatgt tttcgaaagc cgttatatgg caaacaatgg cggcatcagt
      481 tacatgtaca cacaaaccaa atggaatgct acagcgggtt tgtcgtatca ggttgccaca
      541 cttaaaaacg aacaaacgtt tccacaatcc gacaatttaa ataaaacatt ttacagcttg
      601 ttgcctaatg cgcagtttca gtataagttt actacttcaa aaaatatgcg cgtaaattat
      661 agaagcagta ataatgcacc atcggttaca caattgcaaa atgtggttaa caacagtaac
      721 ccgctgcaac ttacagccgg taatcccaat ttaagtcaag actggcaaaa caacgttaac
      781 atacgatact cgtccgttaa cacgctaaaa agcaccaatt tatttgcagt tttaaatctt
      841 actaaaaccg aagactacat tgccaatacc acctactttg ctttaaccga tacacaagtt
      901 gccggtgtaa atatgccggc aggttcgcag ttaactgtgc cgcaaaactt aaacggatat
      961 tacaatgtgc gtatgtttag taattacggt tttcctttta aagctattaa atcaaaccta
     1021 aacataaatg ccaatgccaa cctgaaccgc acacccggtc aaataaattt tgtaaacaat
     1081 tatgcatata caacaagtgt tggaaccggt ttctccataa acagcaacat cagccccaag
     1141 attgattttt ctgtaggcag ttacttttca tataacaaca taactaatac ccaacaatcg
     1201 caagccaaca acgaatattt aaatctgaat actcgtttac gtatgcagtt tacaatctgg
     1261 aaaggcttgg tacttcaatc agatgtaacc aaccaacact atgcaggttt ggccgataat
     1321 ttaaatcagg attttatatt atggaatgcc ggtataggtt ataagttttt gaaaaatgat
     1381 gcggctgagt tacgattaac cgtgtttgat attttaaatc aaaacaacag tttcaatcgt
     1441 aacacaactg aaacctattt tgaagatgta actaccaatg tgcttacaca gtacttcatg
     1501 ataaatttta cttataacat taagtacttt aaaaatgcag gcaaagaaaa ttaagcagca
     1561 tttaccttta atgttttatt aattattttg atggcttatg catttattaa aaacttgcat
     1621 ccatatttta aaatgtaagc catgaaaata caccgattca tcatacttat ttttgcgtta
     1681 agttttcagt ttaattgtac tacggcacaa tctgaaaaca catatacaca aacaatcagc
     1741 ctttcagata ataaacaaaa cctgcgctta actttaggtt cggataagta ctaccgttta
     1801 attgtacagt atgccgatac acctaaaatg cccgaagctt atgcaggtaa ttacatacga
     1861 accaaaaatg tggttgcatt aacgggcaat gtaccataca aatattttag tttgaatgat
     1921 aataccatgc agtggtgtga tgaaacaggc agggctttaa acaatcaaac tgttttaact
     1981 aatgatacag gtatgcatac atcattagat aaacgtgatg aacagaaaat aaaaaatggt
     2041 atagactttt tt
//
LOCUS       DAOMAA010000362         2051 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_53885_length_2051_cov_2.634770, whole genome shotgun sequence.
ACCESSION   DAOMAA010000362 DAOMAA010000000
VERSION     DAOMAA010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53885_length_2051_cov_2.634770"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="PLO59_05600"
     CDS             <1..301
                     /locus_tag="PLO59_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019388642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HQV00614.1"
                     /translation="CAGTHNEDSSMLLVNLMKHRNIASNHPHIWFSQLLGMSDHISFN
                     LSKAGYNVCKYVPYGPVRDVMPYLLRRAKENTSVKGQTGRELSLIMKEKARRKSL"
     gene            340..>2051
                     /locus_tag="PLO59_05605"
     CDS             340..>2051
                     /locus_tag="PLO59_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQV00615.1"
                     /translation="MLVLNNITFEFGSRALYDQASWHIKPNEKIGLIGANGTGKSTLL
                     RIINGEYQLAGGEITGRKDVSIAFLNQDLLSYQSEDNILKVAMEAFEVQNRLHDEIQI
                     ILDKLEYDHSEGTLNKLHDLQHEFEVLDGYNIQYKAEQVLEGLGFTTAQLQKPLNTFS
                     GGWRMRVMLAKILLQQPDLLLLDEPTNHLDLPSIEWLENYLTTYTGTFIIVSHDRFFL
                     DKVVTKIAEVAQSKITLYTGNYSFYLEEKAERQEMQRAQFKNQQKFIKEQERLIDRFK
                     AKASKASMAQSRMKMLAKLERFDDVEESDGAIKVNFNFDKQPGKIIAQVNVKSKAYAD
                     VEVLTNTSLTINRGDKIALIGANGRGKSTLLRIINNSEPFEGKHEWGHQVVPSFFAQH
                     QLESLNLKLNPLEELQHCAPEQTDNNLRGLLGAFLFEGDDVFKKIAVLSGGEKSRVAL
                     AKTILTKANFLLLDEPTNHLDMKSVNVLIRVLNRYEGSFVCVSHDRFFLSQIANKIWY
                     IENKELKEYPGNYEEYQIWLEEKAAQANNKTNVAKAEAKQEAIVKETEVKKSEDKERK
                     KQHQ"
BASE COUNT          726 a          373 c          413 g          539 t
ORIGIN      
        1 ttgtgccggt acgcacaatg aagatagcag catgctgttg gttaacctga tgaaacatcg
       61 caatattgcc tcaaaccacc cccacatttg gttttcgcaa ttgttaggca tgagcgacca
      121 tataagtttt aatctctcaa aagcaggcta taacgtatgc aaatatgtgc cttatggccc
      181 tgttcgcgat gttatgcctt atttactcag acgtgctaaa gaaaacacct ccgttaaagg
      241 acaaaccgga cgcgagctga gtttaattat gaaagaaaaa gcgaggcgca aatcgcttta
      301 attctaataa ttcatttttg cgaaattttt attttacaca tgttagtatt aaataacatc
      361 acattcgagt ttggctcgag ggcattgtat gaccaagcct catggcacat taaaccaaat
      421 gaaaaaattg gtttgatagg cgccaatggt acaggcaagt caacactgct gcgcattatc
      481 aatggcgagt atcaattagc cggtggcgaa attacaggcc gtaaagatgt gtctattgca
      541 tttttaaatc aggacctatt gagctaccaa agcgaggata atatacttaa agtagccatg
      601 gaagcttttg aagtacaaaa ccgcttgcat gacgaaattc aaataatatt agacaaacta
      661 gaatacgacc acagcgaagg caccttaaac aaattgcacg atttgcaaca tgagtttgaa
      721 gtgttagatg gttacaacat tcaatacaaa gccgaacaag tgctagaagg gttgggcttt
      781 acaacagcgc aacttcaaaa gccattaaat acttttagcg gaggttggcg catgcgtgta
      841 atgcttgcaa aaatactgtt gcaacaacct gatttgttat tgttagacga gcctacaaat
      901 cacctggatt taccttcgat agagtggctc gaaaattatc ttacaaccta taccggcact
      961 tttataatcg tatcgcacga caggtttttt ttagataaag tagttacaaa aattgccgaa
     1021 gtagcacaaa gcaaaatcac actttatacg ggcaactaca gtttttattt agaagaaaaa
     1081 gccgagcgac aagaaatgca acgtgctcaa tttaaaaacc aacaaaaatt tattaaggaa
     1141 caagagcgct taattgatcg gtttaaagcc aaagccagta aagccagtat ggcacaatcg
     1201 cgtatgaaaa tgctggcaaa gttagagcgg tttgatgatg tagaagagtc ggatggtgct
     1261 attaaagtaa actttaattt cgataaacaa cccggcaaaa ttatagcgca agtaaacgta
     1321 aagtctaaag cttatgccga tgtagaagta cttaccaata catcgttaac cataaaccgt
     1381 ggcgataaaa tagcattaat tggtgctaac gggcgtggta aatcaaccct actgcgcatt
     1441 ataaacaaca gcgaaccatt tgaaggaaaa catgagtggg ggcatcaggt tgtaccttcg
     1501 ttttttgcac aacatcagtt agaaagttta aaccttaaac ttaatccgtt agaagaattg
     1561 caacattgcg cacccgagca aactgataat aatttgcgtg ggttgttagg tgcattttta
     1621 tttgaaggtg atgatgtatt taaaaaaatt gctgttttaa gtggaggcga aaaatcgcgt
     1681 gtggccttag ctaaaacgat acttacaaaa gccaattttt tattgttaga cgagcctacc
     1741 aatcacctag atatgaaaag cgttaacgta cttatacgcg ttttaaatcg ttatgaaggt
     1801 agttttgttt gcgtatcgca cgaccggttt tttctatcac aaattgccaa caaaatttgg
     1861 tatatcgaaa ataaagaact gaaagagtac cccggcaatt acgaagagta tcaaatatgg
     1921 ttagaagaaa aggccgcaca ggcaaataat aaaaccaatg ttgcaaaagc cgaagcaaaa
     1981 caagaagcca tagtaaagga aactgaagta aaaaaatcag aagacaagga gcgtaaaaaa
     2041 caacatcaaa a
//
LOCUS       DAOMAA010000363         2050 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_53921_length_2050_cov_3.376441, whole genome shotgun sequence.
ACCESSION   DAOMAA010000363 DAOMAA010000000
VERSION     DAOMAA010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53921_length_2050_cov_3.376441"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="PLO59_05610"
     CDS             <1..742
                     /locus_tag="PLO59_05610"
                     /inference="COORDINATES: protein motif:HMM:NF025430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HmuY family protein"
                     /protein_id="HQV00616.1"
                     /translation="ASIIRGDCNNINYFNLNDVHDTIGCNWKYDSPGWETDSTAFGQW
                     SMPTGNPVYIIERSLVSPASPQDRFWKMTILQSNSTAYTISYARLTDTVSRILTLPKI
                     ANQNYTHFSFNNGGSMVSIEPDKQKWDIVFSRYRFIYHDFSPPLPYYVNGVLLNAHLT
                     FAAVDSVTPYDSIHYAQAVNYTLKNKRDVIGFDWKIFNFTSQKYEVKPYYVYIIKDQE
                     GYYYKLHFLSFYDPMGIAGAPAFEFHRL"
     gene            complement(758..1888)
                     /gene="hemW"
                     /locus_tag="PLO59_05615"
     CDS             complement(758..1888)
                     /gene="hemW"
                     /locus_tag="PLO59_05615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HQV00617.1"
                     /translation="MAGIYFHIPFCKQACHYCNFYFSTNRRQQQSLVTALIKELETQK
                     NFFENGTVVQTIYFGGGTPSLLTIDEVHQIMEAIKKYFVVLPQAEISFECNPDDISFE
                     QCTAWLRAGVNRLSIGIQSFVDAHLQLMNRAHNSKQALQSVENAVAAGFKNISIDLMY
                     ALPNLSDAEWKQNILTACSLPINHISCYNLTIEEKTALAKFIATGKCAAIDDEQAARQ
                     FQILLDITGANGFEQYEISNFARQQQYSAHNTAYWQSKPYLGIGPSAHSFKNNKRYAN
                     IAHTAQYVQAIAEGNLRQEIETLTPAMQYNEWVLTRLRTCWGVDVALLETQFGYEKAQ
                     CFKLQMSTAIMLGRVKLYNNCYTLTALGKLWADDVAQSAFIV"
     gene            complement(1925..>2050)
                     /locus_tag="PLO59_05620"
     CDS             complement(1925..>2050)
                     /locus_tag="PLO59_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQV00618.1"
                     /translation="RGWGLGLSLSKRIIEEYHKGQIFVKQSEQNKGTTFRIVLPK"
BASE COUNT          610 a          409 c          367 g          664 t
ORIGIN      
        1 tgcatcaatc ataaggggtg attgtaataa tatcaattac tttaatttaa atgatgtaca
       61 cgatacaatt ggatgtaact ggaaatatga cagccctggc tgggaaaccg actctacagc
      121 ctttggtcaa tggagtatgc ccacaggcaa tccggtttat ataattgaac ggagtttggt
      181 ttcaccggca tcgccacaag atcgcttttg gaaaatgact attttacaat caaacagcac
      241 agcatacact ataagttatg caaggttaac cgatacagta agccgaattt taacgttgcc
      301 taaaattgcg aatcaaaact atacacattt tagttttaat aatggtggca gcatggtgag
      361 tatcgaacct gacaaacaaa aatgggacat tgttttttca cgctaccgtt ttatatatca
      421 cgatttttcg cctccgttgc cttactatgt aaacggtgtg ctgctaaatg cccatttaac
      481 ttttgctgcc gttgatagcg taacgcctta tgatagcata cattatgcgc aagcggttaa
      541 ttacacttta aaaaacaagc gcgatgttat tggcttcgac tggaagattt ttaattttac
      601 aagtcagaag tatgaggtaa aaccttacta tgtttatatt attaaagatc aggaaggtta
      661 ttattacaag ttacactttc taagttttta cgaccccatg ggtattgccg gagcacctgc
      721 tttcgaattt caccggctgt aacagttact atatagatta aacaataaat gcactttgtg
      781 ctacatcatc tgcccaaagt ttacccaatg cagtaagtgt ataacagttg ttgtacaact
      841 ttactctacc cagcataatt gctgtgctca tttgtagttt aaaacattgt gctttttcat
      901 aaccaaattg agtttcgagc aaagcaacat caacacccca acacgtgcgc aaacgtgtaa
      961 gcacccattc attgtattgc atcgctgggg ttaatgtttc aatttcctgc cgcaagttcc
     1021 cctcagcaat agcctgcaca tactgtgcag tatgcgcaat gtttgcatag cgtttattgt
     1081 ttttaaaact atgcgctgat gggcctatcc ccaagtatgg tttgctctgc caataagctg
     1141 tattgtgcgc actatactgt tgttgtctgg caaagttcga aatttcatat tgctcaaatc
     1201 catttgcacc ggttatatct aataatatct gaaactgacg tgccgcttgt tcatcatcaa
     1261 tagctgcgca tttacctgtg gcaataaact ttgccagcgc tgttttttct tcaatggtta
     1321 ggttgtaaca acttatatga tttattggta agctgcatgc agtaagtata ttctgtttcc
     1381 attccgcatc tgataaatta ggcaaagcat acatcaaatc aatactaata tttttaaaac
     1441 cggcagcaac ggcattttca acactttgca aggcctgctt actgttatgt gcccgattca
     1501 taagttgcaa gtgcgcatca acaaaacttt gtatgccaat acttaaacgg ttaacgcctg
     1561 cacgaagcca ggctgtacat tgctcgaagc tgatatcatc ggggttacat tcgaacgaaa
     1621 tttcggcttg gggcaatacc acaaagtatt tttttatggc ctccattatt tgatgcactt
     1681 catcaatagt aagtaatgaa ggtgtaccac caccaaaata aatggtttgt acaacggtac
     1741 cgttttcaaa aaaatttttt tgtgtttcta attcctttat taatgcagtt actaaacttt
     1801 gttgctgccg cctattggta ctgaaataaa aattacaata atgacaagct tgtttacaaa
     1861 atggaatatg aaaataaatg ccggccattt ttacactgct tctgattgtt gcatcgaatt
     1921 aaaactactt aggtaataca atacgaaaag tggtgccttt attttgttct gattgtttta
     1981 caaaaatttg ccctttatga tattcttcga taatacgttt gcttagcgat aagcccaagc
     2041 cccaacctct
//
LOCUS       DAOMAA010000364         2050 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_53933_length_2050_cov_2.814536, whole genome shotgun sequence.
ACCESSION   DAOMAA010000364 DAOMAA010000000
VERSION     DAOMAA010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53933_length_2050_cov_2.814536"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2050
                     /locus_tag="PLO59_05625"
     CDS             <1..>2050
                     /locus_tag="PLO59_05625"
                     /inference="COORDINATES: protein motif:HMM:NF012836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HQV00619.1"
                     /translation="GRIAPLVSAVGDNVNSTVSVNDYQQNSGTSMSCPAVSGVLTQLY
                     DCYKQTHAGANPQSALIKATLLNTCDDIGNAGADFVYGYGRVNAYKAAVSLENNQHFA
                     ASITQGDSTTFTLNVPSNVSQLKVMLYWHDYEGALNAAPALVNDLDLEVIDDSGNVTL
                     PLVPNSAPNFASLISPATQKIDRLNNQEQAVINNPSGSYTFKVVGFNVPQGPQDFYVV
                     YYFETDSIMVTYPLGGESVAPGELEKIRWDALGNTAPFSVSYSADSGTTWTVINANVA
                     ANRRYLDWTTPTAPSPNYLIKVERNGMEDVSDATFNLMAVPTGLTVDFACPDSIKISW
                     NNVAGAAYYEASRLGAAYMDSNGTSTTNSYVFGGLNPMTEQWFSVKAYHTNGAVSRRA
                     IAIKKDPGIINCALAFDAGVTGISPANLVLQSCVAPSTINLTIGLTNSGVNPISNFDV
                     AYTVNNGTPVIETFTNTINPGANTVYTFTTPINIATPGNYQIAGYHLLAGDGNIFNDT
                     LPANITIINSTPVTLPYVENFESFSQCNTSSDCEATICPLGNDLTNVTNGGGDDIDWR
                     VNSGSTPSSTTGPSFDHTTGNSSGKYVYTEASNGCFFQTAELISPCIDLSNATNPVFS
                     FYYHMFGDDMGDLHVDIFASNAWINDVIPPIVGSQPDDWFKQIVGLSNYNGQTINVRL
                     R"
BASE COUNT          624 a          415 c          413 g          598 t
ORIGIN      
        1 tggccgtatt gcgccattag taagcgctgt tggcgataat gtaaattcaa ctgtatcggt
       61 taacgactat caacaaaact caggcacaag catgtcatgc ccggcagtat caggtgtatt
      121 aacgcagcta tatgattgtt acaaacaaac acatgcaggt gctaatccac aatcggcatt
      181 aataaaagca accttactaa acacatgtga tgacattggc aatgccggag ccgattttgt
      241 ttatggttat ggtcgtgtaa atgcgtataa ggctgctgtt agcttagaaa ataaccaaca
      301 ctttgcagcc tctataacac aaggcgattc aactaccttt acgcttaatg tcccatccaa
      361 tgtatcgcaa cttaaagtaa tgctttattg gcatgattac gagggcgctt taaatgcagc
      421 accagcattg gttaatgatt tagacttaga agttattgat gattcgggca atgtaacttt
      481 accattggtg cctaactctg cacctaattt tgccagttta atttcgccag caactcaaaa
      541 aattgacagg cttaataatc aggaacaagc agttattaat aacccttcgg gcagctacac
      601 ctttaaagtt gtaggcttta atgtaccaca aggcccacaa gatttctatg ttgtttatta
      661 ctttgaaacc gattcgataa tggttacata tccgttggga ggcgagagtg tagcaccggg
      721 cgaacttgaa aaaatcagat gggatgcatt aggcaacact gcaccgttta gtgtttcgta
      781 tagtgccgat agcggtacaa cctggacagt gataaatgca aatgttgctg caaacagacg
      841 ttatttagat tggacaaccc ccactgcacc atcgcctaac taccttataa aagtagaacg
      901 taatggtatg gaagatgtaa gcgatgctac gtttaattta atggcagtac caaccggttt
      961 aactgtagat tttgcatgcc ccgattcaat taaaataagt tggaacaatg tagccggtgc
     1021 agcttactac gaagctagca gattgggtgc tgcttatatg gattcgaatg gcaccagtac
     1081 aaccaacagt tatgtatttg gtggcttaaa cccaatgact gaacaatggt ttagcgtaaa
     1141 agcttatcat actaacggtg ctgtaagtcg cagggctatt gcaattaaaa aagacccagg
     1201 tattatcaat tgcgccttag cttttgatgc aggtgttacc ggtatttcac cagccaattt
     1261 ggttttacaa agctgtgtgg ctccatcaac tattaaccta actattggtt taactaatag
     1321 tggcgtaaat cccataagca attttgatgt ggcttatact gtaaataacg gtacacctgt
     1381 tatcgaaaca tttacaaaca ccattaatcc gggtgccaac acggtttaca catttaccac
     1441 acctattaat attgccacac ccggcaacta tcaaatagcc ggttatcacc tgcttgcagg
     1501 cgatggaaat atatttaacg ataccctgcc ggcaaacatt actattataa actcaacacc
     1561 ggtaacctta ccttatgtcg aaaattttga gtccttttca caatgtaata caagcagtga
     1621 ttgcgaagca accatttgtc cattgggtaa cgatttaacc aatgtaacca atggcggtgg
     1681 cgatgatatt gattggcgtg taaacagcgg tagtacacct tcatctacaa ccggcccttc
     1741 gtttgaccat actaccggta atagcagtgg taaatatgta tatactgagg ccagcaatgg
     1801 ttgtttcttc caaacggccg aattaataag cccttgtatt gatttgagca atgcaacaaa
     1861 ccctgtattt tcattttatt accacatgtt tggcgatgat atgggcgatt tgcatgttga
     1921 catttttgca agcaatgcat ggattaatga tgtaatacct cctattgttg gtagccaacc
     1981 tgatgactgg tttaaacaaa ttgtaggttt aagtaattat aatggacaaa ccatcaatgt
     2041 taggttgcgt
//
LOCUS       DAOMAA010000365         2046 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_54135_length_2046_cov_2.770467, whole genome shotgun sequence.
ACCESSION   DAOMAA010000365 DAOMAA010000000
VERSION     DAOMAA010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54135_length_2046_cov_2.770467"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="PLO59_05630"
     CDS             complement(<1..492)
                     /locus_tag="PLO59_05630"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HQV00620.1"
                     /translation="MSVLVYIESPKGHIKKTGYEVASYAVQVAKTLGTDAIALFIGAA
                     NDAGSAGKYGVNKVLHANASENQEFDAQVYSKMIADIAKENGANIIILANTANGKALT
                     GRLAIKMEAGSVSGTNSLPDVSNGFVITKSVYSGKAIVQYNITTQNKVLSVMGNTLQP
                     QIVA"
     gene            complement(531..1268)
                     /locus_tag="PLO59_05635"
     CDS             complement(531..1268)
                     /locus_tag="PLO59_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HQV00621.1"
                     /translation="MKILVCISNVPDTTSKINFTDDHKSFVSAGVQYIINPYDELALT
                     RALEITEKDGGSVTVINVGDASTDATIRKALAIGATDAVRINAAPRDAYFVAKQIAQY
                     AKQHAFDLILTGRESIDYNGGQVASMVAELLDMPSVTNIKLLQLENGVATMEREIEGG
                     KELLTCPLPLVGSATEGIAEPRIPNMRGIMSARTKPLAVVDAVDVALLTEVIGYDKPK
                     TRSNVNLIAAEDVAKLVTLLHTEAKVI"
     gene            1463..1768
                     /locus_tag="PLO59_05640"
     CDS             1463..1768
                     /locus_tag="PLO59_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQV00622.1"
                     /translation="MDRLTQLLSFYNDDSNDAFTIYALALEYLKSDETKALHYFNLLL
                     KNHADYLPMYYQLGKFYETKNIIDTAVDYYSKGLALAQLQNNKHAINELRSALDDLT"
     gene            complement(1773..>2046)
                     /locus_tag="PLO59_05645"
     CDS             complement(1773..>2046)
                     /locus_tag="PLO59_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015361880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HQV00623.1"
                     /translation="HVAKMAGMPAKVISTANNMLAILEKSHNATHQIKSNRKVEQNEA
                     MQLSFFQLDDPMLEQIREEILKTDINTLTPVEALLKLHDIKKLLGK"
BASE COUNT          572 a          396 c          369 g          709 t
ORIGIN      
        1 tgctactatt tgtggctgaa gagtatttcc catcacggac aatactttat tttgagtagt
       61 gatattatat tgtactatcg ccttgcccga atacactgat ttggttatca caaagccatt
      121 gcttacatca ggaagagaat tggtgcctga cacacttcct gcctccattt tgatagcgag
      181 tcttccggtg agggccttgc cgttggcagt attggctaaa atgatgatat ttgcgccatt
      241 ttctttagcg atatctgcaa tcattttgct gtagacctgg gcatcgaact cctgattttc
      301 agaagcattg gcatgaagca ctttattcac accatatttt ccagcagatc cggcgtcatt
      361 tgcagcccca ataaaaagcg ctatggcatc agtacccaga gttttagcca cctgaactgc
      421 gtatgaagct acttcgtatc ctgttttctt aatatgtccc tttggacttt ctatatatac
      481 taaaactgac atatattttt aatttttgga tgtttttttt gattaatgat ttaaataacc
      541 ttggcttcgg tatgtaacaa ggtaactagc tttgctacat cttcggctgc aatcaagttt
      601 acattgctac gggttttggg tttatcgtaa ccaattactt ctgttaataa ggcaacatca
      661 actgcatcaa ctactgctaa tggtttggta cgcgcactca tgatgcctct catattaggt
      721 atgcggggtt cggctatacc ttcggtagcg ctgcctacta aaggcaaagg acaagtaagc
      781 aattccttac ctccttcaat ttcgcgttcc atagtggcta caccgttttc aagttgtaat
      841 agcttaatat tggtaaccga tggcatatcc aacagttctg caaccataga agctacctga
      901 ccaccgttat agtcaataga ttcgcgcccg gtaagtatca aatcaaaagc atgttgtttt
      961 gcgtattggg caatttgttt tgctacaaaa tatgcatcgc gaggggcagc atttatacgc
     1021 actgcatcgg ttgcaccaat agccaacgct tttcgtatgg ttgcatcggt gcttgcatcg
     1081 cccacattta ttacggtaac cgaaccgcca tctttttcgg ttatttctaa tgcccgggtt
     1141 aaagccagct cgtcataagg gttaattata tattgtacac cagctgaaac aaaactttta
     1201 tggtcatcgg taaaattgat tttcgatgtt gtgtcgggca cattgcttat acaaacaagt
     1261 atcttcatgt ttttatttat tattaagtat tgaggcaaac attattttaa aatcgattgt
     1321 tgtattttaa aacggcagca aacctaaacc attggcaata atataacaag ttactgttaa
     1381 tgctttttaa aaggttttaa atgtttgaca cataagttac tttgctgaaa aaacaattgt
     1441 caatttgata tttgaaatta tcatggatag gcttacgcaa ctattatctt tttataatga
     1501 cgattccaac gatgctttta ccatttatgc tttagcactc gagtatttaa aatctgatga
     1561 aaccaaagca ctccattatt ttaacttact acttaaaaac cacgctgatt atttaccgat
     1621 gtattatcag ttaggcaagt tttacgaaac taaaaatatc attgatactg ctgttgacta
     1681 ttattcaaaa ggtttggcat tagcacaatt gcaaaataat aaacatgcta taaatgagtt
     1741 gcgcagtgca ctcgatgatt taacttagta gcttatttcc ctaatagttt ttttatatcg
     1801 tgtagtttca gcaatgcttc aaccggtgta agcgtattaa tatcggtttt taaaatttct
     1861 tcacgtattt gctctaacat aggatcgtca agctgaaaaa acgaaagctg cattgcctca
     1921 ttttgttcaa cctttctgtt tgattttatt tgatgtgttg cattatgcga tttttcaagt
     1981 atggcaagca tgttattggc tgttgaaatt acttttgcag gcattccggc catttttgcc
     2041 acatga
//
LOCUS       DAOMAA010000366         2042 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_54358_length_2042_cov_2.241570, whole genome shotgun sequence.
ACCESSION   DAOMAA010000366 DAOMAA010000000
VERSION     DAOMAA010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54358_length_2042_cov_2.241570"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1497)
                     /gene="rpsA"
                     /locus_tag="PLO59_05650"
     CDS             complement(<1..1497)
                     /gene="rpsA"
                     /locus_tag="PLO59_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010665027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HQV00624.1"
                     /translation="MNESLNLRTKALGSVGLADDDFDWSGVGNRYDSYKSDEKTRLED
                     MYDKTLMTVAEHQIVNGTVIAVSEKDVVVNIGFKSDGLVQLTEFRHMPDLKVGDVVEV
                     YVETTEDKNGQLILSHKKARSLRAWERVNQALERDDIITGYVKCRTKGGLIVDVFGIE
                     AFLPGSQIDVKPIRDYDVFVGKNMEFKVVKINQEFKNVVVSHKILIEAELESQKIEIM
                     SKLEKGQVIEGTVKNITSYGVFMDLGGVDGLLHITDISWGRIQHPDEVLKLDDKINVV
                     ILDFDGEKKRIALGLKQLTPHPWDNIEEKFSVGDKIKGKVVVLADYGAFIEVMPGVEG
                     LIHVSEMSWSQHLRSPQDFMKVGDEIECVVVSIDKAERKMSLGIKQLTPDPWGTITDK
                     YQVGTKHQAKVRAFTNFGVFVELEEGVDGLVHISDLTWAKKIKHPSEFTKVGDNLEVV
                     VLEVDTENRRLSLGHKQLEDNPWDAYETIFTVDSVHEGTVGKVTDKGAV"
     gene            complement(1648..>2042)
                     /locus_tag="PLO59_05655"
     CDS             complement(1648..>2042)
                     /locus_tag="PLO59_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HQV00625.1"
                     /translation="HPDHGRAAALINDACFLSGLIKIETAYNGQVQQAHRPKAVYHYT
                     QDRYIKPDFLVDISAHVKTKEAALRAYKTQFYNPNSDEPGTYISSPEFFASIFNRNAE
                     FGRQIGVAFAEPFTSYKIIGVKNLFNLL"
BASE COUNT          587 a          445 c          335 g          675 t
ORIGIN      
        1 tacagcgcct ttatcggtta ctttaccaac ggtaccttca tgtactgaat caaccgtaaa
       61 tatggtttcg taagcgtccc aagggttgtc ttctaattgt ttgtgaccta acgaaagtct
      121 gcggttttcg gtatcaactt ctaatacaac tacctctaaa ttatcgccta ctttggtaaa
      181 ctcacttggg tgcttaattt tcttagccca ggttaaatct gagatatgaa ctaagccatc
      241 tacgccttct tctaattcaa caaaaacacc aaagtttgta aaggcacgca ctttagcctg
      301 atgtttggta ccaacctgat atttatcggt aatggtgccc catggatcgg gtgtaagttg
      361 tttaatgcct aacgacattt tgcgttcggc tttgtcaatt gaaaccacaa cacattcaat
      421 ttcatcacct actttcataa aatcttgtgg cgaacgtagg tgttgcgacc agctcatttc
      481 actaacgtga attaaacctt caacgcccgg catcacttca ataaatgcac cgtagtcggc
      541 caataccact actttacctt ttatcttatc acctacacta aatttttctt caatgttatc
      601 ccatgggtgt ggcgttaatt gttttaagcc taaagcaata cgttttttct caccatcaaa
      661 atcaagaatt actacgttaa tcttatcatc cagttttaat acttcatccg gatgctgtat
      721 gcgtccccat gagatatcgg taatgtgcaa taagccatca acaccgccta aatccataaa
      781 tacaccataa gatgtaatat tcttaaccgt accttcaatt acctgacctt tttcaagttt
      841 gctcatgatt tcaatttttt ggctttctaa ttcagcctca attaaaatct tatgcgatac
      901 cacaacgttt ttaaattcct ggtttatttt aaccacttta aactccatat tcttacctac
      961 aaatacatca taatcgcgta taggtttcac atcaatttgt gagcctggta aaaatgcttc
     1021 gataccaaaa acatctacaa ttaaaccacc tttggtacgg catttaacgt agccggtaat
     1081 aatatcatcg cgctccaatg cctggttaac gcgctcccat gcacgtaatg accttgcttt
     1141 tttatgcgat agaattaatt gtccgttttt atcttctgta gtttcaacat aaacttctac
     1201 aacatcgcca acttttaaat cgggcatgtg tctgaattcg gttagctgca ccaatccatc
     1261 cgatttgaaa ccgatgttta ctacaacatc tttttctgaa actgctataa cagtaccgtt
     1321 tacaatttga tgttcggcta ctgtcattaa tgttttatcg tacatatctt ccaagcgcgt
     1381 tttttcatca ctcttgtaag aatcgtaacg attgcccaca cctgaccaat caaagtcatc
     1441 atcggctaaa ccaacacttc ccaaagcttt ggttctaagg tttaaagatt cattcatttg
     1501 ttaaattgtt aaagattaat tttaagccct aaaaagggag cgcaaatgta aacataatca
     1561 gctttgatat tacatgaggc accggacttt tttagcacct gcttaactgt ttgggaaaca
     1621 tcctaaaact caatctaaac aactcaatta taataagtta aataaatttt tgacgccaat
     1681 aattttatag cttgtaaaag gttcggcaaa agcaacgcct atttgacgac cgaactcggc
     1741 atttctatta aatatggaag caaaaaactc gggcgatgaa atgtaggtac cgggctcatc
     1801 gctattggga ttgtaaaatt gggttttgta tgcgcgcaag gctgcttctt ttgtttttac
     1861 atgtgccgat atatctacca aaaaatcggg ttttatgtag cggtcttgtg tgtagtgata
     1921 tactgctttg gggcgatgcg cttgttgtac ctggccattg tacgctgttt caattttaat
     1981 taatcctgat aagaaacaag catcatttat taatgcagcg gcacgtccat ggtcggggtg
     2041 cc
//
LOCUS       DAOMAA010000367         2037 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_54628_length_2037_cov_2.416751, whole genome shotgun sequence.
ACCESSION   DAOMAA010000367 DAOMAA010000000
VERSION     DAOMAA010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54628_length_2037_cov_2.416751"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="PLO59_05660"
     CDS             <1..556
                     /locus_tag="PLO59_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00626.1"
                     /translation="SLCCEAKALSIFLKEKNYTAATGSLQEAINKINDIIGGQILSSL
                     PKTSGGLNCDAASDQVTSLGTMMGAGMIVSRNYLSEDGKMVSLQITPNSPALESLNTF
                     LDNPNDYGTESEAGKSIKVGKHKALSRFTNEGDNTTGYLQMPFYTSVLVFQGNGFKTE
                     DDFIAFVAGLNYDGLAKSMGFVKE"
     gene            complement(623..1249)
                     /gene="tmk"
                     /locus_tag="PLO59_05665"
     CDS             complement(623..1249)
                     /gene="tmk"
                     /locus_tag="PLO59_05665"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007094052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HQV00627.1"
                     /translation="MAHNFFIALEGIDGSGKSTQTKLLHHLLSKAGMQVQIDFEPTDN
                     EIGRLLRHIFSGKINANQYTIAALFAADRLDHILNPKNGLQKKLAEGYTVICDRYYLS
                     SLAYHSVHVPIDWVMDCNKMAMQLLKPHLHVFVDVPPALAMQRLKQSRPQLEMYETLS
                     NLEAVYAKYQEAIEKINAEENIFRVDGTQNPDSISALIFEELQRRFSI"
     gene            complement(1323..>2037)
                     /locus_tag="PLO59_05670"
     CDS             complement(1323..>2037)
                     /locus_tag="PLO59_05670"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HQV00628.1"
                     /translation="AANQWAFNYMINHYGKPVDRSEWSMQPQTYNAYYNPSYNEIVVP
                     ACNIIVPGFEGRMPDDAILYGIIGGSTFGHEFTHGFDDQGSLYDENGNLNNWWTVADS
                     TQFKARTKLIVDQYNQYVVLDSLHVNGDATQGENIADLGGLVMGYEAFKKTEQGKSNE
                     HICGYTPDQRFFLAYAYAWMVNRRPESLARQIMSDVHAPSEFRVNGPVSNMPQFYNAF
                     NIKPGSLMHRADSMRVVIW"
BASE COUNT          596 a          406 c          413 g          622 t
ORIGIN      
        1 aagcctttgc tgcgaggcaa aggctttgag tatattttta aaagaaaaaa attatacagc
       61 agcaaccggt tcgttgcagg aagccatcaa taaaattaat gatattatag gtgggcaaat
      121 tttatcatcg ctacctaaaa ccagtggggg tttaaattgc gatgcagcca gcgatcaggt
      181 taccagttta ggcaccatga tgggtgcagg tatgattgta tcgcgtaatt acctgagcga
      241 agacggtaaa atggtgagtt tacaaataac acctaactcg cctgcattag aaagtttaaa
      301 tactttttta gataacccta acgattatgg taccgaaagc gaagccggta aaagtattaa
      361 agtaggcaag cataaagcat taagccgctt tacaaacgaa ggcgataaca ctaccggtta
      421 tttgcaaatg cctttttata catcggtttt ggtttttcaa ggcaatggct ttaaaaccga
      481 agatgatttt attgcatttg ttgcaggctt aaactatgat ggactggcaa aatctatggg
      541 ctttgttaaa gagtaaacct tatacaaata aacacacaaa gaggctgtct caaaatccaa
      601 atttgagaca acctctttgt ttttagatcg aaaacctacg ttgtaattct tcaaaaatta
      661 aagcactaat actgtcgggg ttttgtgtac catctaccct aaaaatattt tcttcggcat
      721 taattttttc aatggcttcc tggtatttgg cataaaccgc ttcgaggttg cttaaggttt
      781 catacatttc taattgcggg cggctttgct ttaacctttg catggccaat gccggtggca
      841 catctacaaa aacatgtagg tgtggtttta ataactgcat ggccatttta ttacaatcca
      901 ttacccaatc tattggcaca tgtacgctgt ggtaagctaa agatgaaagg tagtatcggt
      961 cgcaaattac ggtgtaacct tcggccagtt ttttttgcaa tccgttttta gggttcagaa
     1021 tatgatcgag cctgtcggct gcaaatagtg cggcaatggt atattgattg gcattaattt
     1081 ttccgctaaa aatgtggcgc aacaaacggc caatttcatt atcggttggt tcgaaatcaa
     1141 tttgaacctg catacccgct ttacttaata aatgatgcaa tagcttggtt tgtgtactct
     1201 taccactacc atcaatacct tctaatgcaa taaaaaaatt atgagccatg ctgttaaagt
     1261 ttgtacacaa ctttttacac tatgattaaa ttaaaactgc tgttgtgtaa agtgtttata
     1321 gtttaccaga ttacaacacg catgctatcg gcacggtgca ttaggctgcc gggcttaata
     1381 ttaaatgcat tataaaactg tggcatgtta cttacagggc cgtttactct gaattcggat
     1441 ggcgcatgta catcgctcat tatctgacgc gccaaacttt caggcctacg gtttaccatc
     1501 caggcataag cataagccag gaaaaagcgc tggtcgggcg tatagccgca aatatgctca
     1561 ttgctctttc cttgttcggt tttcttaaaa gcttcgtaac ccataactaa gccacctaag
     1621 tcggcaatgt tttcgccttg cgtggcatcg ccatttacat gcaaactatc taacacaaca
     1681 tattgattgt attggtcaac aattaattta gtacgcgctt taaactgggt actgtcggca
     1741 actgtccacc aattgtttaa gttgccgttt tcatcataca agctaccctg atcgtcaaag
     1801 ccatgtgtaa actcatgacc aaaggtagag ccaccaataa tgccgtacag tatggcatca
     1861 tcgggcatgc gcccttcgaa accgggtaca attatattgc aggccggtac tacaatttcg
     1921 ttataacttg ggttgtagta agcattgtaa gtttgtggtt gcatgctcca ttcgctgcgg
     1981 tcaacgggct tgccgtaatg attaatcatg taattaaaag cccattgatt ggcggcc
//
LOCUS       DAOMAA010000368         2036 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_54679_length_2036_cov_2.372539, whole genome shotgun sequence.
ACCESSION   DAOMAA010000368 DAOMAA010000000
VERSION     DAOMAA010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54679_length_2036_cov_2.372539"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="PLO59_05675"
     CDS             complement(<1..587)
                     /locus_tag="PLO59_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00629.1"
                     /translation="MIKLFKIASTYPKYADGFFNSHQQLRHATYQQALQAYFEDCFAW
                     ADFWKINLEATNQFQVCEVVFNNEFLQKKWAIENNFKFDDWMPQILFAQLNQFKPDVI
                     FIQDIYHYAHWYVDIKKRYPFIKLVMAWDGILWHKTQTYADVDVVLSYVQQSVDFYKQ
                     HNKTAYYFVSGFETTIAGKLQQHKNCNVSFSGSLV"
     gene            complement(584..1294)
                     /locus_tag="PLO59_05680"
     CDS             complement(584..1294)
                     /locus_tag="PLO59_05680"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HQV00630.1"
                     /translation="MMYRFYNKIKRKLFKGNTIYTKGVFNSAINTSLYKCNINVGAGA
                     KLTIAEHVNMQYVNIEVTEGQVYIGEHCMLNGTKNSPVHISVNGALHIEHHNVVYGNI
                     TVRFNGVCNIGSYNGIMHQTEIRCDESINIGSFNMISYDCMIYDTNTHCQLDAEIRQQ
                     QTINDYPWIGKEYQKPVTAPVIIGSHCWLGKRSVVLKGVIIGNGVTVGAQAVVTKNIS
                     DGALAYGNPAIIKMKNVL"
     gene            complement(1298..>2036)
                     /locus_tag="PLO59_05685"
     CDS             complement(1298..>2036)
                     /locus_tag="PLO59_05685"
                     /inference="COORDINATES: protein motif:HMM:NF025877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Wzt carbohydrate-binding domain-containing
                     protein"
                     /protein_id="HQV00631.1"
                     /translation="VAAHLEPEILIVDEVLAVGDAEFQKKCIGKMKDVSGSGRTILFV
                     SHNLPSLSSLCNKGMVLHKGTVNLPLSPIQDAISHYTKITQHLAQSVISQRTDRQGEG
                     GIRFTAFNLLNAQMQPIDIAVCGQTVIFKLDYCCNKFEAKQIAAAIGIYGLDGQLFTV
                     FANEYAQGNFATAPNSGSMYCTIEKFPLTSGKYALNLIVYCNGIMHDWVQQAAMIEVD
                     DGDFYGTGKTVASTHRSILVAQKWKME"
BASE COUNT          602 a          418 c          351 g          665 t
ORIGIN      
        1 aaaacaagcg aacctgagaa tgaaacattg caatttttat gttgttgtag tttcccagca
       61 atggttgttt cgaaaccgct tacaaaataa taggcagtct tattgtgttg cttgtaaaaa
      121 tctaccgatt gctgcacata actcagcacc acatcaacat cagcataagt ttgtgtttta
      181 tgccacaata tgccatccca agccattacc agttttataa atggataacg tttttttata
      241 tccacatacc aatgtgcata gtgataaata tcttgaataa aaattacatc cggtttaaat
      301 tggtttaact gggcaaataa tatttgtggc atccaatcgt caaatttgaa gttgttttca
      361 attgcccatt tcttttgcaa aaactcatta ttaaatacca cttcgcaaac ctgaaactga
      421 ttggtagctt ctaaattaat tttccaaaaa tcggcccatg caaaacaatc ctcaaagtaa
      481 gcctgcaatg cttgctggta tgtagcatgg cgcaattgct gatggctgtt aaaaaatcca
      541 tcggcatact ttgggtaggt agatgctatt ttaaaaagtt taatcacaat acgtttttca
      601 tttttatgat tgccggattt ccataagcca aagctccatc tgaaatgttt ttagtaacta
      661 cagcctgtgc gccaacggtt accccgtttc cgatgatcac accttttaaa actacgctgc
      721 gtttgccaag ccaacaatga ctgccaataa taactggagc cgttaccggt ttttgatatt
      781 ctttgcctat ccagggatag tcgttaatgg tttgttgctg ccgaatttca gcatctaact
      841 ggcaatgcgt gttggtatca taaatcatgc agtcgtacga tatcatatta aagcttccaa
      901 tatttatact ttcatcacat ctgatttcgg tttgatgcat tatgccatta tagctaccaa
      961 tgttgcacac tccattaaac cgtaccgtaa tattaccata aaccacgttg tggtgttcga
     1021 tatgcagtgc gccatttacg ctaatatgca ccgggctatt tttagtacca ttcagcatac
     1081 agtgttcacc tatgtaaacc tggccctcgg ttacttcaat attaacgtat tgcatattta
     1141 catgttcggc aatggtaagt tttgctccgg cacctacatt aatattgcac ttatacagcg
     1201 aagtatttat tgcactatta aaaacacctt tggtataaat agtatttcct ttaaaaagtt
     1261 tacgcttgat tttattataa aaacgataca tcattgatta ctccattttc catttttgtg
     1321 caactaaaat acttcggtgt gtagatgcta cggttttacc ggtaccgtaa aaatcgccat
     1381 catctacctc aatcattgct gcttgctgta cccaatcgtg catgatacca ttgcagtaca
     1441 caattaggtt taacgcatat tttcctgatg taagcggaaa tttttcgata gtacaataca
     1501 tgctgccgct gtttggcgct gttgcaaaat tgccttgggc atattcgtta gcgaatacgg
     1561 taaaaagttg tccatctaaa ccatatatac caatagctgc agcaatttgt tttgcctcaa
     1621 atttgttgca gcaataatct aatttaaaaa taacggtttg gccacatacg gcaatgtcga
     1681 taggttgcat ttgcgcattt aaaagattaa aagcagtaaa ccgaatacca ccttcgcctt
     1741 gcctatcggt acgttgcgat ataacggatt gagccaaatg ttgcgtaatt ttagtatagt
     1801 ggctaatggc atcttgtatg ggcgatagcg gcaaatttac agtgccttta tgtaatacca
     1861 ttcctttatt gcaaagactg cttaatgaag gcaaattgtg gcttacaaat aagatggtac
     1921 ggccgctacc cgaaacatct ttcattttgc caatacattt tttttgaaac tcggcatcgc
     1981 caactgccaa cacttcatca actattaaaa tttcgggctc gagatgtgca gcaaca
//
LOCUS       DAOMAA010000369         2034 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_54789_length_2034_cov_2.917130, whole genome shotgun sequence.
ACCESSION   DAOMAA010000369 DAOMAA010000000
VERSION     DAOMAA010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54789_length_2034_cov_2.917130"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..>2034)
                     /locus_tag="PLO59_05690"
     CDS             complement(151..>2034)
                     /locus_tag="PLO59_05690"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HQV00632.1"
                     /translation="VTGGTLVSGQATDTAIITWGSAGTGTITVTEITDLGCPSQPVVL
                     TVSINQILTPVAPNGTLSICANDGLGTAYSTLNTPGSTYQWFVIGGTVASGNGTNSIT
                     VDWNAAGPSIGYVWYQEQSITPTTTCLGLSDTLQVVINPAPATSAITANAAICVFDST
                     AFSVINTTGSNYQWFANGSSSLSGNGTNNIIAGWSTPGTYTVGVVETNSFGCVGDTVP
                     YSITINALPAANAGNNTGFCLGQTTAQLNASGGIGYTWQPATGLSATNIANPVANPGA
                     TTTYTVTVTDANGCSATASLQVTANALPVAGISATPAAICIGSSTQLQASGGNSYTWL
                     PATALSNTAIGNPVANPTAATTYTVTVTDANGCTGTTSYLINVNPLPTISAGNDVLIC
                     DNASITLSASGGLSYTWSPATGLSSSTIANPIATPSGLTQYTVTGTDANGCTNTDTII
                     VSINPKPVANFATDSTYASCSGVTVYFTDLSTDADSYLWNFNDGTTSTDANPIHAFAF
                     AATNNVTLIVGNNGICFDTLTAPLALKSLSDYVKNAANVFTPNGDGKNDCYSFSGLGD
                     FSNCTNIKIFDRWGKQQFSSSGTEKCWDGKSEGGKEVPVGTYFYIIDVAGKQINGSLQ
                     LLR"
BASE COUNT          573 a          422 c          434 g          605 t
ORIGIN      
        1 atttgttatc aattttatag cagcgaaaca aatagtttta catgcaaata gtttcgagta
       61 tttgtttgcc agaaatcatt attgagaaat tgaattaaaa aaaagaggga tacaaatttg
      121 tatccctctt ttaatattta atgttttaaa ctatcgtaac aattgcagcg agccatttat
      181 ttgttttccg gcaacatcaa ttatgtagaa gtaagtacct accggtacct ctttgccacc
      241 ttctgatttg ccatcccaac atttttcagt accactactg ctaaactgtt gtttacccca
      301 acgatcaaaa attttaatgt tggtacaatt actaaaatcg cctaagccgc taaaactgta
      361 acaatcgttt ttgccatcac cgttgggtgt aaacacattg gcagcattct tcacataatc
      421 gcttaatgat tttaatgcca atggtgctgt aagtgtatcg aaacatatac cgttgttacc
      481 tacaattagt gttacattat ttgtagcggc aaaagcaaat gcatgaattg gatttgcatc
      541 ggttgaagtg gtgccatcat taaaattcca taagtatgaa tcggcatcgg tacttaaatc
      601 tgtaaaatat acggttacgc cactgcaact cgcataagta ctgtcggttg caaagtttgc
      661 caccggcttt ggatttatgc ttactattat ggtgtcagta ttcgtacatc catttgcatc
      721 ggtaccggtt acagtatatt gtgttaatcc actgggtgta gcaatggggt tagcaatggt
      781 actactgctt aaacctgtag caggtgacca tgtataagat aaaccacctg aagcgcttaa
      841 ggtaattgat gcattgtcgc aaataagtac atcgttaccg gccgaaatgg taggtaacgg
      901 atttacatta ataagatacg atgtggtacc tgtacatccg tttgcatctg ttacagttac
      961 cgtataggta gttgctgcag tagggttggc aaccgggtta ccaatagctg tgttgcttaa
     1021 tgcagtagca ggcagccagg tataggaatt acctccgctc gcttgtaatt gtgtactgct
     1081 gccaatacat atagctgcag gtgttgccga aataccggca acaggtaatg cattggcagt
     1141 tacttgtaag ctggcagtag ccgagcaccc attggcatcg gttactgtta ctgtataggt
     1201 agtggtagct cccgggttag caaccggatt ggcaatgtta gttgccgata aaccggtggc
     1261 tggttgccag gtataaccaa tgccaccgct tgcatttaat tgtgccgtag tttgacctaa
     1321 acaaaaaccg gtgttattgc ctgcattagc tgccggcaat gcatttatgg taatactgta
     1381 aggcacggta tcgcccacac aaccaaagct attggtttca acaacgccta cggtataggt
     1441 gccgggtgta ctccagccgg caattatatt attggttcca ttgccactta aactactgct
     1501 gccattggca aaccactgat agtttgaacc tgtagtattt ataacactaa acgctgtact
     1561 atcgaaaaca caaatagcgg cattagctgt tatagccgat gtagccggtg ccggattaat
     1621 aactacctga agcgtatcgc ttaaacctaa acaagtggta gtaggtgtta tactttgctc
     1681 ctgataccat acatagccaa ttgatgggcc ggctgcattc caatcaaccg taatgctatt
     1741 ggtgccattg ccgctggcaa ccgtaccgcc aataacaaac cactgatagg ttgaacccgg
     1801 tgtatttaaa gttgaataag ctgtacctaa accatcgttg gcacaaattg ataacgtgcc
     1861 attgggtgca acaggagtaa gtatttgatt aatacttact gttagcacaa ctggttgcga
     1921 tggacaacct aagtcggtaa tttcagtaac cgtgatagtg cctgtacccg cagatcccca
     1981 ggttattatg gctgtatctg tggcctgtcc gcttacaagg gtgcctccgg taac
//
LOCUS       DAOMAA010000370         2027 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_55140_length_2027_cov_2.628803, whole genome shotgun sequence.
ACCESSION   DAOMAA010000370 DAOMAA010000000
VERSION     DAOMAA010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55140_length_2027_cov_2.628803"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="PLO59_05695"
     CDS             <1..379
                     /locus_tag="PLO59_05695"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV00633.1"
                     /translation="ITALNIALQNIDSAYKAGVTGPTAAMLTCPGAPRIASNLGVTTT
                     SVLVYPNPSAGVVNVKLETEVEGTVVINMLDITGRAVWNTQEPIFTGENVRTYDFSQL
                     TKGVYMMQVIKDGKSEIIRVVLR"
     gene            492..1916
                     /locus_tag="PLO59_05700"
     CDS             492..1916
                     /locus_tag="PLO59_05700"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQV00634.1"
                     /translation="MKYLRIEQLRIEYQSGFNSPIFNLKIEKGQWLLLSGSSGSGKTT
                     LAKAIMHLLPPDAQVTGSIFLDNIDLLLPTQIDNVLKRGIIRYMPQNTGLFFNPYKNA
                     FTQLFDAVNIYNKTHALHKTREILEVVQLPFLIEKKKHAFQLSGGEQQRLLLALSLAS
                     APELLIIDEPTASVDRKLKTQLIELIKSLQQQLGITILSISHEEDLYKPLVNRHYKIE
                     DGCIIPYQQTITKPVLKLNNKDAKREIVLSCTNINYSFKAPTKQFLLLNDVSLQLFSN
                     SITGLSGASGAGKSTLAKILNKTLEPDSGRIFLCNNEINGNNKSAQKKFQKQVQLLMQ
                     DALASLNPKHTLEQILLAPLLYHKIYFGKEEARSQISKWLHLLHLSDTVLQNYPHQLS
                     MGQLQRMLFVRNFTLKPLITIVDECFTALDEANKQLIINAMKLYIMQNGASCLLISHD
                     YNLLQQVCNYRYDIADGVLQPVGT"
BASE COUNT          702 a          345 c          376 g          604 t
ORIGIN      
        1 aattacggca cttaacatcg cacttcaaaa tattgacagt gcatacaaag caggtgttac
       61 aggtccaaca gcagcaatgc taacttgtcc tggcgcgcca cgcattgcta gtaatcttgg
      121 tgtaactaca acttctgtat tggtttatcc taatccttca gcaggtgttg taaatgtgaa
      181 gttagaaaca gaggttgaag gcacagttgt aatcaacatg cttgacatta ccggacgtgc
      241 tgtatggaat acacaagagc caatatttac cggtgaaaat gtgcgtactt atgactttag
      301 tcagttaact aaaggcgttt atatgatgca ggtaattaaa gacggcaagt cggaaattat
      361 acgtgttgta ttgcgttaaa aggtatagat tcctattaca caaagcctcg gtcaattact
      421 tgatcggggc tttgtgtttt aaattatatt ttcgaccaaa agatttacaa tgctattaat
      481 gcaacagcgc gatgaaatat cttagaattg aacaactaag aatcgaatat cagagcggtt
      541 ttaattcgcc catattcaat ttaaaaattg aaaagggcca atggctattg cttagtggaa
      601 gctcaggaag tggcaaaacc acattggcaa aagcaataat gcatttgttg ccgcctgatg
      661 cgcaagtaac aggttcaatt tttcttgata atatagattt attgcttccc actcaaattg
      721 acaatgtttt gaagcgtggc attattagat acatgccaca aaacacaggc ttgtttttta
      781 acccatataa gaatgccttt acgcaattgt ttgatgctgt aaacatttac aacaaaaccc
      841 atgcattaca taaaaccaga gaaattttag aggtagtgca attgccgttt ttaatagaga
      901 aaaaaaaaca tgcatttcag ctttcgggtg gcgagcagca aagattactc cttgcgctct
      961 cgctggcatc tgcacctgag cttttgataa ttgatgaacc aactgcatct gttgatcgaa
     1021 aacttaaaac acaattgatt gaattaatta aaagcttgca acaacaatta ggcattacta
     1081 tacttagtat ttctcatgag gaggatttat ataagccact ggtaaaccgg cattataaaa
     1141 ttgaagatgg ttgtattata ccctatcaac aaactataac aaaaccggtt ttaaaattaa
     1201 ataacaaaga tgcaaagcgt gaaatagtat tatcatgcac aaatatcaat tacagtttta
     1261 aagcgcccac aaaacagttt ttgcttttaa atgatgttag tttacaatta ttcagtaata
     1321 gtataacagg tctgagtggg gcatcggggg cgggaaaaag cacattggca aaaattttaa
     1381 ataagacatt agaacccgat tcggggcgta tttttttatg taataatgag attaacggga
     1441 ataataaatc ggctcaaaaa aaatttcaaa aacaagtaca gttgctgatg caagatgcct
     1501 tggcatcgct caaccctaag catacattag aacaaatttt attagcacca ctgctgtatc
     1561 ataaaatata ttttggtaaa gaggaagcac gctcacaaat ttcaaagtgg ttacatttat
     1621 tgcatctgtc agatacggtt ttacaaaatt acccgcatca gcttagtatg ggtcaattac
     1681 agcgtatgtt gtttgttcgg aattttacat tgaagccact aataacaata gtagatgaat
     1741 gttttacggc attggacgaa gccaacaagc aattaataat taatgcaatg aaattgtaca
     1801 tcatgcaaaa tggagcaagc tgtttactaa tttcacacga ttataattta ctgcaacaag
     1861 tttgtaacta cagatatgat atagccgatg gcgtactgca accggttggc acctaattta
     1921 actttttgaa taaacaaaaa acaattttta aaaatatgag taacaataag caagtaattg
     1981 ttttaggaag cggcctggtt ggctcactat taagcattat tttaacc
//
LOCUS       DAOMAA010000371         2026 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_55173_length_2026_cov_3.700152, whole genome shotgun sequence.
ACCESSION   DAOMAA010000371 DAOMAA010000000
VERSION     DAOMAA010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55173_length_2026_cov_3.700152"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="PLO59_05705"
     CDS             <1..520
                     /locus_tag="PLO59_05705"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HQV00635.1"
                     /translation="FTETNQRPEIFAYGLRNPWRFSFDNKTGRLFCGDVGQNKFEEIN
                     LIENGKNYGWRGMEAFEVYDPEINLPDAVLPIYAYPHSVGNSVTGGYVYNGSQIAWLK
                     GAYVYADWSGKLFNLTQNTAWKNFNLDVKNMSALGNINALGIDANGELYVLMQQLTGP
                     FDKTGVLYKIAP"
     gene            552..875
                     /locus_tag="PLO59_05710"
     CDS             552..875
                     /locus_tag="PLO59_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010421075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HQV00636.1"
                     /translation="MKKLPPFELIVWIVVITYLGFLNPYQETTSLCAFGWLGLTWCPG
                     CGIGHSISFALHGNISASFESHYLGIIALIILLHRIFILSKQYYQNLTLNNPTHGQEP
                     TDDVT"
     gene            844..1191
                     /locus_tag="PLO59_05715"
     CDS             844..1191
                     /locus_tag="PLO59_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010599373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HQV00637.1"
                     /translation="MDKNQLMMLPNIEAEEIMFLQNVTKDLTADQDRNFFMIYQSRRK
                     DPQTIMLVTILGFVVVAGIQRFMLNQIGMGLLYFFTGGLCLIGTIIDLINFKKMTFTY
                     NQQMAMEALQICR"
     gene            1230..1823
                     /locus_tag="PLO59_05720"
     CDS             1230..1823
                     /locus_tag="PLO59_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HQV00638.1"
                     /translation="MAHLKVLAKDKYLKKILPAEGLVLTTRKNVFLSLCNSIMSQQLS
                     TKVADIIQQRFVVLVGSKSPVPKQVWQLPDTDLRNIGLSNAKVAYIKNVAHFAINNGM
                     ALRQLNKMTDEEVIAYLTQIKGVGRWTVEMLLMFTLGRENVFAVDDLGIQQAMINLYK
                     LDATNKKQLKATMLQHAQNWHPYRTYACLYLWKYKDA"
     gene            complement(1824..>2026)
                     /locus_tag="PLO59_05725"
     CDS             complement(1824..>2026)
                     /locus_tag="PLO59_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013622120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQV00639.1"
                     /translation="LVQDALIKLMQHRTTLVIAHRLSTIQHADEIIVLQRGQIVERGN
                     HQQLIAMQGVYKKLYDMQGFAV"
BASE COUNT          640 a          355 c          395 g          636 t
ORIGIN      
        1 atttactgaa acaaaccaac gccccgaaat ttttgcttat ggattacgaa atccctggcg
       61 tttttcattt gataacaaaa caggtcgctt gttttgtggt gatgtaggtc aaaataaatt
      121 tgaagaaata aacctgattg aaaacgggaa aaattacggt tggcgtggta tggaagcatt
      181 tgaagtatat gaccccgaaa taaatttgcc cgatgctgtt ttgccaatct atgcatatcc
      241 gcatagtgtt ggtaatagtg taaccggtgg ttatgtttat aatggttcgc aaattgcttg
      301 gcttaagggt gcttatgttt atgccgattg gagtggtaag ctttttaact taacgcaaaa
      361 taccgcttgg aaaaatttta atctggatgt aaaaaacatg tctgcattag gcaacataaa
      421 tgcattgggt attgatgcaa atggcgaact ttatgtgctg atgcaacaat taacaggccc
      481 atttgacaaa accggagtat tgtataaaat agcaccttaa aaccataaac gtattgcagc
      541 tttcaaaact tttgaaaaaa cttccgcctt ttgagcttat tgtttggatt gtggttatta
      601 cgtatttagg tttcttaaat ccgtatcagg aaacaacaag cctttgcgca ttcgggtggc
      661 ttgggctaac atggtgcccg ggttgtggta taggccattc aatttcattt gccctgcatg
      721 gtaacatttc ggccagcttt gaatctcatt acttgggtat tattgcatta ataatattat
      781 tacaccgcat ttttatactt agcaaacagt actaccaaaa tttaaccctt aataatccaa
      841 cccatggaca agaaccaact gatgatgtta cctaacatcg aagccgaaga aataatgttt
      901 ttgcaaaatg taaccaaaga cttaactgcc gatcaggatc gtaatttttt tatgatttat
      961 caaagcagac gtaaagaccc acaaaccatt atgttggtta ctattttagg ttttgttgtg
     1021 gttgccggta ttcagcgttt tatgcttaac caaataggca tgggcttgct ttatttcttc
     1081 accggaggtt tatgtttaat aggtaccatt attgacttga taaatttcaa aaaaatgacg
     1141 tttacctaca accagcaaat ggccatggaa gcattacaaa tttgcaggta atcaatactt
     1201 aacttcacta aaattattaa gtactcttta tggcgcattt aaaagtttta gctaaggata
     1261 aatatttaaa aaaaatattg cctgccgaag gattggtttt aacaacccgt aaaaatgttt
     1321 ttttgagttt gtgtaatagt attatgtcgc aacaactcag tacaaaagtt gccgatatca
     1381 ttcaacaaag atttgtggta ttggttggct ccaaatcgcc tgtgcccaaa caagtttggc
     1441 aactacccga caccgatttg cgcaatattg gattaagtaa tgcaaaagta gcttatatta
     1501 aaaatgtggc acattttgcc attaataacg gtatggcatt aaggcagtta aataaaatga
     1561 ctgatgaaga agtgatagcc tacctgacgc aaattaaagg tgtgggccgc tggacggttg
     1621 aaatgctgct tatgtttaca ctggggcgcg aaaacgtttt tgctgttgac gatttaggca
     1681 tacaacaagc catgataaat ttatataaat tagatgctac caataaaaaa caattgaaag
     1741 ctaccatgct gcaacatgca caaaactggc atccttaccg cacttatgcc tgtttgtatt
     1801 tgtggaaata taaagatgca taactaaact gcaaagccct gcatatcgta cagcttttta
     1861 taaacacctt gcatggctat tagctgctga tgattgccac gttcaacaat ttggccacgt
     1921 tgtaaaacaa taatttcatc ggcatgttgt atggtgctta agcggtgggc aataactaaa
     1981 gtggtgcggt gttgcatcag tttaattaat gcatcctgca ccaaac
//
LOCUS       DAOMAA010000372         2024 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_55300_length_2024_cov_2.877095, whole genome shotgun sequence.
ACCESSION   DAOMAA010000372 DAOMAA010000000
VERSION     DAOMAA010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55300_length_2024_cov_2.877095"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="PLO59_05730"
     CDS             <1..396
                     /locus_tag="PLO59_05730"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV00640.1"
                     /translation="WSNGKTSKTIGGLTIGTTYTATVTDANGCTVSGSILFTGNRLGD
                     NVTSFTANLYPNPTQDVVTLSLFNCPLKHATIEIKNMMGAVVLKQKLLVHNENQSILI
                     ETINFDQGVYDVSILIDDKILNQRLIIQR"
     gene            472..1035
                     /locus_tag="PLO59_05735"
     CDS             472..1035
                     /locus_tag="PLO59_05735"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HQV00641.1"
                     /translation="MSISKGFTLPLIVLGILLLLFGFISVTAPNMSVQTLMTYLAFML
                     LATGVLSVIVSLLIRKSGKFWWISMLFGFIAIVFSYYIFTHAEVAAHYYTVFIASWAG
                     FMGLALIAASFFQKHLQVILIVNGLMSLGFGLVIYFNPFSGANTLNFMVGFYTILLSI
                     MILYLAYYMVKPKSKPSELNTDNTLKK"
     gene            complement(1045..>2024)
                     /locus_tag="PLO59_05740"
     CDS             complement(1045..>2024)
                     /locus_tag="PLO59_05740"
                     /inference="COORDINATES: protein motif:HMM:NF036482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00642.1"
                     /translation="KPVNSYFDDVPRFATEFGMPSLPGISTLNNYVKAESIDDIQVSQ
                     MMRSYKGVSALKKYTLSGYTEPNSLANWVYTTQCMQAEAVLKAINAQRMKKPFCMGSM
                     YWQIADCWPGITWAAIDFDKNYKALQYKLPQAFSTYAIAAQCSEEKICVSLINDGPKN
                     KSATVELYIKNFNGAVLWQHTLHVKLKTDINVTLTLDSILPKIKQWGQGNVYLLGLMY
                     DAAGLLANQHYFFDETKKLQIPEPTIAADVCAYGNDYLLTINSKYLVKDVFCNVYNAK
                     ADFSNNYFDLLPNESQQVIIKTTLPINQLIESISLKTSNGMVTINHSTN"
BASE COUNT          612 a          343 c          335 g          734 t
ORIGIN      
        1 tggagtaatg gtaagacatc aaaaaccatc gggggcttaa ctattggtac tacctatact
       61 gcaacagtaa cagatgccaa tggttgtact gtaagtggca gtatattgtt taccggcaat
      121 cgtttgggtg ataatgttac atcgtttact gcaaacttat atcctaaccc aacgcaggat
      181 gtggttacct tatctctatt taattgccca ttgaaacatg ccaccataga aattaaaaac
      241 atgatgggtg ccgttgtttt aaaacaaaaa ctgcttgtgc ataatgaaaa tcaaagtatc
      301 ctaatcgaaa caataaactt tgatcaaggt gtttacgatg taagtatttt aatagatgat
      361 aaaatattaa accagcgatt aataatacag cgttagtatt tttttttaga ggccccgctt
      421 aggcggggct tttattttta tatcattgtc gaaatcaaca cacaatcaca tatgagtatt
      481 tctaaaggat ttacattgcc attaatagtt ttgggtatat tactgctatt gtttggtttt
      541 attagcgtaa ccgctccaaa tatgagtgtt caaacattaa tgacctattt ggcctttatg
      601 ttacttgcta ccggtgtgtt atcagtaatt gtaagtttat taataagaaa gagtggtaaa
      661 ttttggtgga tttctatgct ctttggcttt atagcgattg ttttttctta ctacattttt
      721 acccatgccg aagttgccgc acattattac accgttttta ttgcttcatg ggccggcttt
      781 atgggcttgg cattaatagc agcttcgttt tttcaaaaac atttgcaagt tattttaatt
      841 gtcaacggcc ttatgtcatt aggctttgga ttggtaattt atttcaatcc attttcgggc
      901 gctaatactt taaattttat ggttggtttt tatacaatat tattaagcat aatgatatta
      961 taccttgcat attatatggt aaaaccaaag tctaaacctt cagaattaaa cactgacaat
     1021 actttaaaaa agtaattaac tactttaatt tgttgaatgg tttatggtaa ccattccatt
     1081 tgaagttttt aaactaatgc tttcgattaa ttgatttatt ggaagcgttg tttttataat
     1141 aacttgttgt gattcgtttg gtaataagtc gaaataatta ttactaaagt ccgctttggc
     1201 attgtaaaca ttacaaaaaa catcttttac caaatattta ctatttatgg ttaacaagta
     1261 atcattacca taagcacaaa catcagcggc tattgttggt tcaggtattt gcagtttttt
     1321 tgtttcatca aaaaagtaat gttgattagc cagtaaacca gcggcatcat acatcaaacc
     1381 caataaataa acattgcctt gcccccactg tttaattttt ggcaaaatag aatctaatgt
     1441 cagcgttaca ttaatatccg ttttaagttt tacgtgtaac gtgtgttgcc acaatacagc
     1501 tccattaaaa tttttaatat acagctcgac tgtagcagat ttatttttag gcccatcatt
     1561 tatcaacgaa acgcaaattt tttcttcact gcactgtgca gcaattgcat aagttgaaaa
     1621 agcctgtggt agtttatatt gcaatgcttt ataattttta tcgaagtcaa ttgcagccca
     1681 ggtaataccc ggccagcaat cagcaatttg ccaatacata gaacccatac aaaatggctt
     1741 tttcatgcgt tgtgcattta ttgcttttaa aactgcctct gcttgcatac actgtgttgt
     1801 atatacccaa ttggctaaac tattgggttc agtataaccg cttaaagtat atttttttaa
     1861 tgcgcttact cctttataac tgcgcatcat ttggcttacc tgtatatcgt caatagattc
     1921 tgcttttacg taattattca atgtagatat tcctggcagc gatggcatac caaactctgt
     1981 tgcaaatctg ggtacatcgt caaaataact gtttacgggt ttat
//
LOCUS       DAOMAA010000373         2014 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_55789_length_2014_cov_3.527820, whole genome shotgun sequence.
ACCESSION   DAOMAA010000373 DAOMAA010000000
VERSION     DAOMAA010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55789_length_2014_cov_3.527820"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="PLO59_05745"
     CDS             complement(<1..186)
                     /locus_tag="PLO59_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQV00643.1"
                     /translation="MRILVTGGTGFIGSHTAVALIENGHDVFIVDNLNNSRAEVVQSI
                     NKITGKAVIFSTIDCCDF"
     gene            complement(461..1921)
                     /locus_tag="PLO59_05750"
     CDS             complement(461..1921)
                     /locus_tag="PLO59_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00644.1"
                     /translation="MDILNQIIQSLNKEEIRFYKLLAQRTQVGGVRKDVKLFDEMRKK
                     GDAFDEEVFLKKHYNHQTKNAYYRLKNRLLNDVSESLALHHFSDDEQGYALHLLALEK
                     YFSTQNKFKVAHYFLKKAETLALKNENFQLLDIIYAEYIKLSHELFQVNPETYIHKRT
                     ANLQNINSLKQIDDILAAVAYRLKTTQNFKQGDVSIMQVLQKTIDDFSNDAALKKSPV
                     LRLKIYQAVSQLLLQRRAYLALEEYLLKLYNDFSAEGLFTKANHDTHLQMLTYIVNSL
                     FKNNKINQSLQYTEQLKQAMEQYNHLHYDKYFFFYYNSLVINYSVIDKPKAIEILNQL
                     KSHKQIKSNQYYEIFVYLNLAVQHFDMRQYHEAIRALNKLYVLQSYKNTDNALKFKIA
                     LADLIIRFELQDFDVFDYKLAQMRKDFGDLLKQKENIKEATFLKLIVVLAKQPWQQLT
                     AKNQAQMLAFIDTYSLAQQADDEILGYVAWLKSKVK"
BASE COUNT          597 a          359 c          347 g          711 t
ORIGIN      
        1 aaaatcacaa caatcaattg tgctaaatat tacggctttg ccggttattt tattgatgga
       61 ctgaaccacc tcggcccgcg agttgtttaa attatctaca ataaaaacat catgtccatt
      121 ttctattaag gctacagcag tatgcgagcc aataaaaccg gtaccaccgg taactaaaat
      181 acgcatttga taaaatataa aaattaaaac ggcaacgaat gtaaaaacac ctttttataa
      241 gccgatgtta ccgtttggta aagagcaaaa aaaatcgcct ttgtttccaa aagcgatttt
      301 tagcgattgc tgatttcatg ccttttatca gcgataacag tactacccct aatactgcat
      361 agcaatgtta aacaggttta tgctgcatgt ggctatccgt tttaaaagat tattacgcat
      421 tcagaccact taaaagatgt gcagttgaag gtaggaccgg ctattttact tttgatttta
      481 gccaggccac atagcctaaa atttcgtcat cggcttgttg tgctaaacta taggtatcaa
      541 taaaagccag catttgggct tggttttttg cagtaagttg ctgccagggt tgtttggcta
      601 atactactat aagttttaaa aacgttgctt cttttatatt ttctttttgc tttaataaat
      661 cgccaaaatc tttacgcatc tgggcaagct tataatcaaa cacatcaaaa tcctgtagct
      721 caaacctgat tattaaatcg gctaaagcaa ttttaaactt cagcgcattg tctgtgtttt
      781 tgtagctctg caaaacatac aatttattta gtgctctgat agcttcatgg tattggcgca
      841 tatcaaaatg ttgtacagcc agattgaggt acacaaatat ttcatagtac tgattgcttt
      901 taatttgctt atggcttttt aactgattta atatttcgat agccttgggt ttatcaatta
      961 ccgagtagtt tataaccaat gaattgtagt agaaaaaaaa gtacttatca taatgcaaat
     1021 ggttgtattg ctccattgcc tgttttaact gttcggtgta ttggaggctt tggtttattt
     1081 tattgttttt aaacaatgag ttaacaatgt aggtaagcat ttgcaaatgc gtatcgtggt
     1141 tggctttggt aaacaatccc tcggcactaa aatcattata cagttttagt aaatactctt
     1201 caagtgccag gtatgccctg cgttgtaaca aaagctggct tacggcctgg tatattttaa
     1261 gcctcaaaac cggacttttt ttaagtgctg catcgttact aaaatcgtca atagtttttt
     1321 gcaatacctg cataatactt acatcgccct gcttaaaatt ttgtgttgtt ttaagcctgt
     1381 aagccactgc agccagtata tcgtcaattt gtttgaggct gtttatgttt tgcaaattgg
     1441 ctgtacgctt gtgtatatac gtttcggggt ttacctgaaa caattcatgc gatagtttaa
     1501 tgtattcggc ataaataata tcgagcaatt gaaaattttc gtttttaagc gctaacgttt
     1561 ctgccttttt taaaaaatag tgggctacct taaatttgtt ttgcgtgctg aagtatttct
     1621 caagtgctaa caagtgcaaa gcgtaacctt gttcgtcatc gctgaaatgg tgtaaggcca
     1681 ggctttcgga cacgtcattt aacaagcggt tttttaaacg gtaatatgca tttttggttt
     1741 gatgattata atgttttttt aaaaagactt cctcatcaaa agcatctcct ttctttcgca
     1801 tctcatcaaa caattttaca tctttacgta caccgcctac ttgggtacgt tgcgccaaaa
     1861 gcttgtaaaa ccttatttct tctttgttta aactttgtat tatctgattt aaaatatcca
     1921 taaaacctat gcaaaatgta taacaataaa atgcaaccta tttgcttata cggttaaagt
     1981 tatttatgcc gtaacccttt tgctaaaata catt
//
LOCUS       DAOMAA010000374         2004 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_56350_length_2004_cov_2.751154, whole genome shotgun sequence.
ACCESSION   DAOMAA010000374 DAOMAA010000000
VERSION     DAOMAA010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56350_length_2004_cov_2.751154"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="PLO59_05755"
     CDS             complement(<1..794)
                     /locus_tag="PLO59_05755"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00645.1"
                     /translation="MQFKNIIGQQAIKNRLIQSVQNERLPHAQLFYEHEGSGGLALAV
                     AYAQYANCLNPGPDDSCGTCSACHKINKLIHPDIHFAVPTAADSKREKLARTSDYAGI
                     WQQSFLNNPYLNLNDWFADLNFETNKQLFLSVEESADIIKKLSYKNFEARYKFCIIWC
                     PEKWNTSAANALLKLIEEPPPATIFILVTEHYDQIISTILSRTQLVKIPRLTQDELIT
                     VLQAEQIPIEKQASLIHLSNGSYRLLQDLMHESQDDNYATDFLVWMR"
     gene            969..>2004
                     /locus_tag="PLO59_05760"
     CDS             969..>2004
                     /locus_tag="PLO59_05760"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010600982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HQV00646.1"
                     /translation="MKTVADYNFKDKKALVRVDFNVPLDKGTLAVTDDTRIRAAIPTL
                     QKILNDGGSIILMSHLGRPLKKLNDDGTIAINKHSLKHTVPVIESLLKTTVIFVDDCM
                     GDAAAAAAKKLMPGQVLLLENLRFYKEEEKGDVTFAQKLAAYGDVYVNDAFGTAHRAH
                     ASTAVIAQFFPNHKLFGFVMANELASIKKVLENPARPFTAIMGGAKVSDKILIIEQLL
                     QKVDHLIIGGAMAYTFAKAKGGKIGTSLCEEDKLPLALEIIEKAEKAGVKLHLPVDSV
                     NAESFSNDARKVGQSSIMQIPDDLMGLDIGNNSVALFVEVIRQSKTILWNGPMGVFEM
                     KPFQQGTIAIA"
BASE COUNT          614 a          381 c          405 g          604 t
ORIGIN      
        1 cgcatccaaa ccaaaaaatc agttgcgtaa ttatcatcct ggctttcatg catcaaatcc
       61 tgcaataaac gatagctgcc attgcttaaa tgaattaaac ttgcttgttt ttcgataggt
      121 atttgttccg cttgtaacac cgttattagt tcatcctgcg ttaggcgggg tattttaacc
      181 agctgtgtgc gcgatagtat ggttgatatt atttggtcgt aatgctcggt aactaaaata
      241 aaaattgtgg ctggtggcgg ttcttcaatt agtttaagta aagcatttgc agcactggta
      301 ttccattttt cgggacacca gatgatgcaa aatttataac gggcttcaaa gttcttatag
      361 cttagctttt taataatatc tgcactctct tcaacactta aaaacaattg tttattggtt
      421 tcgaaattta aatcggcaaa ccaatcgttt aagtttaggt atggattgtt taaaaaagat
      481 tgctgccata tacctgcata atcgctggta cgtgccagtt tttcacgttt gctatcggca
      541 gcagtaggca ctgcaaagtg tatatcgggg tgtattagtt tatttatttt atgacaggca
      601 ctgcaagtac cacaactatc atcaggcccg gggtttaagc agttggcata ttgtgcataa
      661 gccaccgcca aggccaagcc tccacttccc tcatgttcat aaaaaagctg ggcatgcggc
      721 aacctttcgt tttgcaccga ttgtatgagc ctgtttttaa tggcttgttg acctataatg
      781 tttttaaatt gcacgtataa agcgtattaa cctgttgttt ttattgtttg acgattatca
      841 atgggcccca aaactaattt tttgaaacgg ttaacagaat ttatatcata gtagcagcta
      901 tttctttaat tctattttcg cccccgcatt ttacaaacca taaatactta atttaatacc
      961 atttaaaaat gaaaacagta gccgattata atttcaaaga taaaaaagcc ttagtgcgtg
     1021 ttgattttaa cgtgccgtta gataaaggca ccttggcagt aacagacgat acgcgcatca
     1081 gagcagccat accaactttg caaaaaatac tgaacgatgg cggcagtata attttaatgt
     1141 cgcatttagg acgaccacta aaaaaattaa atgacgatgg taccattgca ataaacaaac
     1201 actcgctaaa acataccgta cctgttattg aaagcttact taaaaccaca gtaatttttg
     1261 ttgatgattg tatgggcgat gcagccgctg ctgcggcaaa aaaactaatg cccggtcagg
     1321 ttttactttt agaaaacttg cgcttttaca aagaagaaga aaagggcgat gtaacttttg
     1381 cacaaaaact agctgcttat ggtgatgttt atgtaaatga tgcttttggt acagcacacc
     1441 gtgcacatgc cagcacggct gttattgcac agttttttcc gaaccataaa ttgtttggat
     1501 ttgtaatggc taatgaactg gcaagcataa aaaaggtact cgaaaatccg gcccgtccgt
     1561 ttacagcaat catgggtggc gcaaaagtgt cggataagat tttaattatt gaacaacttt
     1621 tgcaaaaagt agatcatctg attattggtg gcgctatggc ttacactttt gccaaagcca
     1681 aaggtggtaa aataggcacc agcttatgcg aagaagataa attacccctg gctcttgaaa
     1741 ttattgagaa ggccgaaaaa gccggagtaa aactacactt gccggttgat agcgttaatg
     1801 ccgaaagctt tagtaacgat gcacgcaaag taggccaaag cagcattatg caaatacctg
     1861 acgatttaat gggtttagat attggaaaca atagtgtagc cctttttgtg gaggttatcc
     1921 ggcaatcgaa aaccatacta tggaatgggc ccatgggcgt tttcgaaatg aagccgttcc
     1981 aacaaggaac catagccatc gcca
//
LOCUS       DAOMAA010000375         2000 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_56551_length_2000_cov_3.146530, whole genome shotgun sequence.
ACCESSION   DAOMAA010000375 DAOMAA010000000
VERSION     DAOMAA010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56551_length_2000_cov_3.146530"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="PLO59_05765"
     CDS             <1..890
                     /locus_tag="PLO59_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019987010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HQV00647.1"
                     /translation="GTIVGVPAWLQMLLEKIIDRYQLKNIHDIWPNLSLFVHGGVAFE
                     PYKKSFLPLLGKPINYMETYLASEGFIALQEYPDQGMNLVLNNGLFFEFIPFNDLNFT
                     ADGDLKGKPQTLLINEVEEDVEYALLLSSNAGAWRYLIGDVIKFTNKQRCEIAIIGRT
                     KHYLSLCGEHLSVDNMNKAIEMVSEELNISIPEYTVAGIPYESLFAHKWFMGCDDKVQ
                     PDVLRQLLDEKLKVLNDDYRVERTSALKEVIVEVLPTSVFLDWMSARGKVGASHKFPR
                     VMKKQIIQEWETHIQRYKV"
     gene            893..1504
                     /locus_tag="PLO59_05770"
     CDS             893..1504
                     /locus_tag="PLO59_05770"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HQV00648.1"
                     /translation="MLSAISDGFFVGVALAFLIGPVFFMLIQTSIEHGLRQAIALAFG
                     VLVSDALFILIAELGSNIFFNNNMVAGYVGYIGGVILIAFGAVNFYKKPSQTHVSVQS
                     KTDVLKTIIKGFAMNSFNPFVLIFWLGVAGSLAGKSYESNMHVVFYASVLTTVFGTDI
                     LKSYLATKLQRLLTATVLVWMNRISGVALVLFGMRIIYKSWLL"
     gene            complement(1501..>2000)
                     /locus_tag="PLO59_05775"
     CDS             complement(1501..>2000)
                     /locus_tag="PLO59_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008631927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQV00649.1"
                     /translation="NPHVVLMDIDMPGVNGIEGLKLIHKQFPDVLIIMQTVFEDDDKI
                     FDAVHAGANGYFLKKTPPQRLIDGIRDVTEGGAPMTATVARKVLEMFQKQPLHKGVKD
                     FDLTQREIDILGMLMKGLSYKMIAEQCSISYNTVNSHCKNIYEKLHVHSATEAVAKVR
                     NHSLF"
BASE COUNT          580 a          355 c          412 g          653 t
ORIGIN      
        1 taggtaccat tgtaggtgta cctgcctggc tgcaaatgct gctcgaaaaa attattgatc
       61 gttaccagct aaaaaacata cacgatatat ggcctaacct gagtttattt gttcatggtg
      121 gtgtagcttt tgagccttac aaaaaaagtt ttttgccttt gttaggaaag cccatcaatt
      181 atatggaaac ctatttggca tcggaaggat ttatagcact gcaagaatac cccgatcaag
      241 gcatgaatct ggttttaaac aacggcttgt tttttgagtt tatacccttt aacgatttaa
      301 attttacggc cgatggcgat ttaaaaggca aaccacaaac gctgctcatt aacgaagttg
      361 aagaagatgt agagtatgca ctgttgttat ccagcaatgc aggcgcatgg cgttatctta
      421 taggtgatgt aattaaattt acaaacaagc aacgatgtga aattgcaatt ataggtcgta
      481 ccaaacatta cctgagcctt tgtggcgaac atttatcagt tgataatatg aataaagcaa
      541 ttgaaatggt aagcgaagaa cttaatattt cgattcctga atatactgtt gccggtattc
      601 cctatgaaag tctttttgcc cataaatggt ttatgggttg tgatgataag gtgcagccgg
      661 atgtactgcg tcaactactt gatgaaaaat taaaagtact taatgacgat taccgtgttg
      721 agcgtacctc tgctttaaaa gaagtaatag tagaagtatt gcctacttcc gtatttttag
      781 actggatgtc ggcacggggc aaggtgggtg cttcgcacaa gtttccgagg gttatgaaaa
      841 aacaaatcat ccaagagtgg gaaacacata tccaacgcta taaagtataa atatgctatc
      901 ggccataagc gatggatttt ttgtaggggt agcactggcc tttttaattg gccctgtatt
      961 tttcatgctc attcaaacca gtattgagca tggcttaagg caagctattg ctttggcatt
     1021 tggcgtattg gtaagcgatg ccttatttat tttaattgcc gagttaggca gtaatatttt
     1081 ttttaacaat aatatggtag ccggttatgt tgggtatata ggtggcgtta tattaattgc
     1141 atttggcgca gtaaactttt ataaaaaacc atctcaaacg catgtatcag tgcaaagcaa
     1201 aaccgatgtg ctaaaaacca ttataaaagg ttttgctatg aattcgttta acccttttgt
     1261 tttaattttt tggttaggcg tagcaggctc gttggcaggt aaaagttacg agtcgaacat
     1321 gcatgttgtt ttttacgcaa gtgttttaac aaccgttttt ggcaccgata ttttaaagag
     1381 ttatttggca accaaattac agcgtttgtt aactgcaacc gtactagttt ggatgaaccg
     1441 tatttcgggc gtagcattag ttttgtttgg catgcgcata atttacaaaa gctggttact
     1501 ttaaaataat gaatgattgc gcactttggc tactgcttcg gttgctgagt gcacatgtaa
     1561 tttttcataa atatttttac agtgtgaatt aaccgtgttg taactgatac tgcactgctc
     1621 ggcaatcatc ttataactta aacctttcat aagcataccc agtatatcaa tttcgcgttg
     1681 ggttaaatca aaatctttaa cgcctttatg taatggctgt ttctgaaaca tctctaatac
     1741 cttacgtgca acggttgccg tcatgggtgc gccaccttcg gttacatctc taataccatc
     1801 aattaagcgt tggggtggtg ttttctttaa aaaatagcca ttggctccgg catgtacggc
     1861 atcaaaaatt ttgtcgtcat cttcaaatac ggtttgcata attatcagca catccggaaa
     1921 ctgtttatgt atcagtttta agccctcgat accatttacg cccggcatat caatatccat
     1981 caataccaca tgcgggttgg
//
LOCUS       DAOMAA010000376         1998 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_56662_length_1998_cov_2.529079, whole genome shotgun sequence.
ACCESSION   DAOMAA010000376 DAOMAA010000000
VERSION     DAOMAA010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56662_length_1998_cov_2.529079"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..363)
                     /gene="rpsR"
                     /locus_tag="PLO59_05780"
     CDS             complement(82..363)
                     /gene="rpsR"
                     /locus_tag="PLO59_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HQV00650.1"
                     /translation="MATEQSPNNSEIRYLNPVNVDTTRKKYCRFKKLGIKYIDYKDEK
                     FLMRFVNEQGKMLPRRITGTSLKFQRKVGQAVKRARHIAILPYVADLLK"
     gene            complement(374..733)
                     /gene="rpsF"
                     /locus_tag="PLO59_05785"
     CDS             complement(374..733)
                     /gene="rpsF"
                     /locus_tag="PLO59_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HQV00651.1"
                     /translation="MLKQYETVFILNPVLSQDQLNETVEKFKRILTEGGAKIVFENNW
                     GLRKLAYPIQKKNTGFYYLIEFQAPGELIAKLEVEYKRDERVMRFLTTALDKHAVAYN
                     EKKRKNAVTKAAETTNA"
     gene            complement(853..>1998)
                     /locus_tag="PLO59_05790"
     CDS             complement(853..>1998)
                     /locus_tag="PLO59_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HQV00652.1"
                     /translation="MGGVLKGVSAVFFAYIGFDAISTTAEECKNPQKDLPKSMMYAIL
                     ICTALYILISLVLTGMVPYYELAVGDPLVFVFEKLNIGWMIGIVGLSAVVAMASVFLV
                     FQLGQPRIWMSMSRDGLLPKAFSRIHPKYQTPSFATIVTCLVVAIPALFLNMTFVTDM
                     VSIGTLFAFVLVCGGVLKLQTDPNAPRSKFKTPYINAQYLMPLLVILCITIAFTKYKA
                     ETMAFISNEGQMYSATEFVNHLSQKDLSDVTYQLSLANPKAFDEAGRDLLAYLETMDN
                     QTYSQNIMQLNLDNHLKIETGWQLFKHKIPTWIFLLITFILTYLAFKHKLSLIPLLGL
                     TCCLYMMSELGYKNWLYFGAWLLIGLIIYFGYSYNNSKLQAKPTLQS"
BASE COUNT          604 a          372 c          370 g          652 t
ORIGIN      
        1 cacgtcatcg gcgtagccaa gtttttctac atcttttata agtataagtt ccattgttta
       61 atctttaatt ggtttaccga tttatttcaa aagatctgct acgtatggca gaatagcaat
      121 gtgacgggca cgttttacag cctggccaac tttgcgttga aacttaagtg atgtgcctgt
      181 aattctacgt ggcaacattt tgccttgctc atttacaaaa cgcattaaaa acttttcgtc
      241 tttataatca atatacttaa tgcctaattt tttaaagcgg caatactttt tacgggttgt
      301 gtcaacgtta accgggttta agtacctgat ttcgctgttg tttggtgatt gttctgtagc
      361 cattttgttt tgattaagcg ttagttgttt cggctgcttt agtgacagca tttttacgtt
      421 tcttttcatt gtatgccaca gcatgcttgt ctaacgctgt tgtaaggaaa cgcattactc
      481 tttcatcacg cttgtactcc acttcaagct tggcaattaa ttcgcccggg gcctgaaatt
      541 cgataaggta ataaaaaccg gtattctttt tctgaatagg gtaagccagt ttgcgtaatc
      601 cccaattgtt ttcaaaaaca attttggcac caccttcggt gaggattctc ttgaattttt
      661 caactgtttc gtttagctga tcctgagaca agacagggtt cagaataaac acagtttcat
      721 actgttttaa cattgtttac ctgttttatt tatatagttt ataaaaaatg gagcgcgaaa
      781 gtagtaatct ctttcgaaac tgcaaaccaa atgcttcgtt caaaaatcct aaggttttaa
      841 aaatacccca tatcaacttt gtaaggtagg ttttgcttgt agtttactgt tgttataact
      901 gtagccaaaa taaattatca aaccaataag caaccaggcc ccaaaatata gccagttttt
      961 ataacccagt tcactcatca tatataagca gcacgttaaa cctaaaagtg gaattaatga
     1021 aagtttgtgt ttaaatgcca agtaagtaag tataaatgtt atcagtagga atatccaagt
     1081 aggtattttg tgtttaaata attgccagcc tgtttctatt tttaagtgat tatccagatt
     1141 tagctgcata atgttttggc tataggtttg gttgtccata gtttcaaggt atgcaagtaa
     1201 atcgcggcct gcttcatcaa aagcttttgg gttggccaaa cttagctggt aggttacatc
     1261 gcttaaatct ttttgcgata ggtggtttac aaactcagtg gcactataca tttggccttc
     1321 attacttata aaagccatgg tttcggcttt gtatttggta aaagctatgg ttatgcaaag
     1381 aattactaat aaaggcataa ggtattgcgc gtttatataa ggagttttaa atttgctgcg
     1441 tggtgcattg gggtcggttt gaagtttaag tacaccgcca cataccagca caaaggcaaa
     1501 caaggtgcct atgctaacca tatcggttac aaatgtcata tttaagaata gtgcaggtat
     1561 agctacaacc aaacaagtta caatggtagc aaaactgggt gtttgatatt tgggatgtat
     1621 gcggctaaaa gccttaggaa gcaggccatc acgactcata ctcatccaaa tacgcggttg
     1681 tcccaattga aaaactaaaa atacactagc catagcaaca acagcactaa ggccaacaat
     1741 acctatcatc caaccaatat ttaatttttc gaaaacaaaa accaatgggt cgccaacggc
     1801 caattcgtaa taaggtacca tgcctgttaa taccaacgaa atgagaatat ataatgcagt
     1861 acatatcaat attgcataca tcatactttt aggtaagtct ttttgcgggt ttttacattc
     1921 ttcggcagta gtgcttatgg catcaaaacc gatgtaagca aaaaacacgg ccgaaacgcc
     1981 tttaagcaca ccacccat
//
LOCUS       DAOMAA010000377         1997 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_56688_length_1997_cov_3.438208, whole genome shotgun sequence.
ACCESSION   DAOMAA010000377 DAOMAA010000000
VERSION     DAOMAA010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56688_length_1997_cov_3.438208"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="PLO59_05795"
     CDS             <1..771
                     /locus_tag="PLO59_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002678910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HQV00653.1"
                     /translation="DSTVTCRMRKADVIDNAHIKAGNVIVGLASYGQSTYETTYNSGM
                     GSNGLTSARHDIFCKSIAQNYPETFDPLLPPHLVYSGQLALTDWVYIHGSEYAVLDNN
                     NQVKLKSNGYNEQLIRHKITAGKMVLSPTRTYAPVINEVLKKFRSKIDGMVHCSGGAQ
                     TKVLHFVKNLHVVKNNMLPVPPLFSIIQNLSATPWHEMYKVFNMGHRMEIYTDSHTAQ
                     SIIEIANQFKIEAQIIGYCESFGGKRLTIETNQGTFQY"
     gene            869..>1997
                     /locus_tag="PLO59_05800"
     CDS             869..>1997
                     /locus_tag="PLO59_05800"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HQV00654.1"
                     /translation="MQVKAGPLLENINYPSDLRKLQTSQLKQVCDELRDFIIDIVSVN
                     GGHFGASLGVVELTVALHYAFNTPDDQLVWDVGHQAYGHKILTGRRDVFYTNRIYKGI
                     SGFPKRSESEYDTFGVGHSSTSISAALGMAVASKLNQNTKKQHIAVIGDGALTAGLAF
                     EALNHGGVSDANLLVVLNDNCMSIDPNVGALKEYLTDITTSETYNKVKDELWKLLGKI
                     SKFGPNAQSIAAKIDSAVKHSLLKQSNLFESLKFRYFGPIDGHDVNHLVKVMHDLKKI
                     PGPKILHCVTVKGKGYELAEKDQTKWHAPGLFDKITGEIFKPKVDQPQPPKYQDVFGH
                     TLVELADRNSKIVGITPAMPSGCSMNIMMKAMPHRAFDVGIA"
BASE COUNT          671 a          381 c          406 g          539 t
ORIGIN      
        1 gattcaaccg ttacatgccg catgcgaaag gccgatgtta ttgataatgc acatattaag
       61 gcgggtaatg ttattgttgg cttggcttca tacggacaaa gcacttacga aaccacctat
      121 aatagcggca tgggcagtaa cggattaaca tcggcaaggc acgatatttt ttgcaaatca
      181 atagcccaaa actatcccga aacttttgac ccactgttac cgccacactt agtatatagt
      241 gggcagttag ccctaactga ttgggtttac atacatggtt cagaatatgc tgtacttgat
      301 aacaataatc aagttaaatt aaaaagtaac ggttataacg agcaacttat caggcataaa
      361 attacagcag gcaaaatggt gctatctccc acacgtacat acgcaccggt gattaacgaa
      421 gtattaaaaa aatttcgttc aaaaattgat ggtatggtac attgcagtgg gggtgcgcaa
      481 accaaggttt tacattttgt aaaaaatttg catgtggtta aaaacaatat gcttccggtg
      541 ccacccttat tcagcattat tcaaaaccta tcggccacac cctggcatga aatgtataag
      601 gtgtttaata tgggccatcg gatggaaatt tataccgaca gccataccgc ccaatccata
      661 atcgaaatag ctaatcagtt taaaatagaa gcacaaatta taggctattg tgagtcattt
      721 ggaggcaagc gccttaccat cgaaacaaat caaggtacct ttcagtatta atagcgtgta
      781 gaaacaatac aaatagctta ttgaacaaaa aagacctttc tttaaaataa tatattcgca
      841 ccattacccc gccaattttg taaatttcat gcaagtaaaa gcaggcccat tattagaaaa
      901 tataaattac ccaagtgact tgcgtaagct ccaaacttcg caactaaagc aagtttgtga
      961 cgagcttcgc gattttatta ttgatattgt atccgtaaac ggaggacatt ttggagccag
     1021 tttaggtgtg gttgaattaa ccgtagcatt acattatgca tttaataccc ccgatgatca
     1081 gttagtttgg gatgtaggtc atcaggcata cgggcataaa atattaaccg gccgtagaga
     1141 tgtattttac accaacagaa tttataaagg tataagcgga tttcccaaac gctctgaaag
     1201 cgaatatgat acttttggtg tagggcattc gtccacatca ataagtgcag ctttaggtat
     1261 ggctgttgca tccaagttaa atcaaaacac aaaaaaacaa catattgccg taattggcga
     1321 tggcgcatta actgccggcc ttgcatttga ggccttaaac catggtggcg ttagcgatgc
     1381 aaatttgtta gtggtactca acgataattg tatgagtatt gaccccaatg tgggcgcatt
     1441 aaaagaatat ttaaccgata ttactaccag cgaaacttat aataaagtaa aagacgaact
     1501 ttggaagctg ttgggtaaaa tatcaaagtt tggtccgaat gcccaaagta ttgcagccaa
     1561 aattgatagt gcagttaaac acagtttgct taagcaaagc aacctgttcg agtcgcttaa
     1621 attcaggtat tttggcccaa tagatgggca cgatgtaaat catttggtaa aagtgatgca
     1681 tgatttgaaa aaaatacccg gacctaaaat tttgcactgc gtaacggtaa aagggaaagg
     1741 atatgaactt gccgaaaaag accagaccaa atggcatgca ccgggtttgt tcgataaaat
     1801 aacaggcgaa atatttaaac ccaaagttga ccaaccccaa ccgcctaaat atcaggatgt
     1861 atttggccac acacttgttg agttagccga tagaaacagc aaaattgtag gtataacacc
     1921 ggccatgcca agtggttgca gtatgaacat aatgatgaaa gccatgccac atcgggcctt
     1981 tgatgttggt attgccg
//
LOCUS       DAOMAA010000378         1994 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_56869_length_1994_cov_2.920062, whole genome shotgun sequence.
ACCESSION   DAOMAA010000378 DAOMAA010000000
VERSION     DAOMAA010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56869_length_1994_cov_2.920062"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1947)
                     /locus_tag="PLO59_05805"
     CDS             complement(<1..1947)
                     /locus_tag="PLO59_05805"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HQV00655.1"
                     /translation="MSHSSCGIVFADDGTLLVPTGDGAAYGGLDNGGGTTTYAAQALA
                     DSIITQPYNVGAFRSQMINSINGKILRIDPSNGNGVPSNPFYDTTNPSSAQSKVWSMG
                     LRNPFRIAIRPGSGSSSAADGKPGVIYIGDVGWAEWEDLHIATKPGQNFGWPIYEGME
                     TNFGYKNLLTPNFFAPNPLYVNGSCMQPFFYFQDLLMQATLDSTKTFKNPCDTSSNVP
                     ATIFTSQHSRPGLTYRHKFDSTLVAAFNGVDAVAYQINDSLAPCQGQVFSGGCIIGGT
                     FYTGNSFPSIYKNKYYMAEFNKGFIKCIATDTNDAPILVDNFANSLGDYVIVHIEQNP
                     HDGSLVYVKYPDEIRKICYACMPDGPPIAKAIADTTFGGDTLLVNFNGSFSTDPDNAP
                     LTYLWSFGDGNTSTLVNPQHLYASQVAGLLTTFTATLTVTDDSSHTDTDTLLIVLNNT
                     PPQVLITTVNDSGLYSVITPTNLILDAQVTDAQHAPSQLFYSWQTTIHINGNDSIIAT
                     DTNKISSVNILPLGCLADTVYYTISLTVTDILGMVGYDKKNIYPDCNAPAAGYYMTAN
                     SQCSFDMVQFTDTSHHFPTAWQWTFTGGNPASSSLQSPVVNYNAQGIFPVVLHVTNAF
                     GTNTISDSISTYNLPIINLLNDTSI"
BASE COUNT          606 a          365 c          416 g          607 t
ORIGIN      
        1 tatagaagtg tcatttaata aattgattat gggcaaatta taagtagaaa ttgagtctga
       61 tattgtattg gttccaaagg catttgtaac atgaagaact acaggaaaaa ttccttgtgc
      121 attgtaatta actacaggcg attgtaaact ggatgatgcc ggattacctc cggtaaatgt
      181 ccattgccat gcggttggaa agtgatgtga tgtatctgta aattgaacca tatcaaaact
      241 gcattgcgaa ttcgctgtca tataatatcc ggcagccgga gcattacaat cggggtatat
      301 atttttttta tcataaccca ccatacctaa aatgtcggta acggttaaag aaattgtata
      361 gtaaactgta tcggctaagc aacctaaagg caaaatattt acgctggata ttttatttgt
      421 gtcagttgca attatggagt cattaccgtt gatatgaatt gttgtttgcc atgaataaaa
      481 taattgtgaa ggtgcatgtt gtgcatctgt tacttgtgca tcgagtatta aatttgttgg
      541 ggttataact gaatataatc cactgtcgtt aactgtggta attaaaacct gaggaggtgt
      601 attgtttagt acaattagaa gtgtatcagt atctgtatgg ctgctgtcgt cagttacggt
      661 caatgttgct gtgaaggtag ttaacaaacc agctacttgc gaagcatata aatgttgcgg
      721 gttaactaat gtagatgtgt tgccgtcacc aaaactccaa aggtaagtta aaggtgcatt
      781 gtcaggatcg gtcgaaaaac tgccgttaaa gtttaccaaa agcgtatcgc caccaaaagt
      841 ggtatcagca atggcttttg caattggtgg cccatcgggc atgcaggcat aacaaatttt
      901 tctgatttca tcggggtatt ttacataaac caaactacca tcatgtggat tttgttcgat
      961 atgcactatt acataatctc ctaaactgtt tgcaaagttg tcaactaaaa tgggcgcgtc
     1021 attggtatca gtagcaatac attttataaa gcccttgtta aactcggcca tataatattt
     1081 gtttttataa attgatggaa aactattgcc ggtataaaat gtaccaccaa tgatgcagcc
     1141 acctgaaaat acttgccctt ggcaaggtgc taatgaatca tttatctgat acgctactgc
     1201 atcaacacca ttaaaagcgg caaccaatgt actatcaaat ttatggcgat aagttaaacc
     1261 gggccggcta tgctgtgaag taaaaatggt ggccggtacg tttgacgaag tatcacatgg
     1321 atttttaaaa gttttagtcg aatctaatgt tgcttgcatt agtaaatcct ggaaataaaa
     1381 aaagggctgc atacatgaac cgtttacata caatggattt ggggcaaaga aattaggcgt
     1441 taataagttt ttatacccga aattagtttc catcccttcg taaataggcc agccaaagtt
     1501 ttgaccgggc ttggtagcaa tatgcaagtc ttcccattcg gcccagccta catcgccaat
     1561 ataaattaca ccgggtttgc catcagcagc actactgctt ccgctaccgg ggcgtatggc
     1621 aatacgaaat ggatttcgta aacccatact ccacactttc gattgagctg aactggggtt
     1681 ggttgtatca taaaatgggt tagaaggaac accgttgccg ttagaaggat caatgcgtag
     1741 tatttttccg tttatactat ttatcatctg agaacgaaaa gcgcctacat tatagggctg
     1801 ggtaataata ctatcagcca aagcttgtgc agcatatgtt gttgtgccgc caccgttgtc
     1861 taaacctcca tatgctgcac catcaccggt aggaacaagt aaggtgccat cgtcagcaaa
     1921 aacaattccg cacgaactgt gtgacatgtg tgttaccgga attccatttt ttttatccga
     1981 ccccaatatt attt
//
LOCUS       DAOMAA010000379         1994 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_56875_length_1994_cov_2.535327, whole genome shotgun sequence.
ACCESSION   DAOMAA010000379 DAOMAA010000000
VERSION     DAOMAA010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56875_length_1994_cov_2.535327"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            409..>1994
                     /locus_tag="PLO59_05810"
     CDS             409..>1994
                     /locus_tag="PLO59_05810"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQV00656.1"
                     /translation="MQAQVTDSLQKLINGPGSNQQKQAWYHAITVFKSKTDAQGALQA
                     AQNEYNFALKNKMPLAQVDALLMFCRIYLQSKNLVPADSVITMAEKLVLQTKDTQMRA
                     QVILYKADIQSDRNEVAEAIQNYTKAFELFEKKLFYKGMAAAINRMALLEFKQGNFES
                     AFKKDQLSFSYSQMTNDSMLMSNAVSNMGNYFYMKQQYDSALYYYNRTLLYMNKNATG
                     RIAMVKSNVGDVLVELQKFKEALPYFIEAKDAFEKAGMVQQLSHLYTSLGRCYVGLKN
                     YNEAETAFNQSLALEEENKDFSLIAENYGHLSDLAALLGNYKKAWEYQLKVEHAKDSL
                     NQQMVNDEVLELQTRFETLQREKDNARLKAENAVHQAETQRQQVLIIALIVSVVLLAL
                     IGLMLFLISRQRQKNLAAQKILNDALTKSKKDVERLNNMLEIRAIRAQMDPHFIYNCM
                     ASALSLLNANRLADTEKYLVKFSRLLRMVLENAEYNAISLDKEIEMLTMYLDLESIRL
                     NANFTYQINVDESLLEENIEV"
BASE COUNT          697 a          332 c          389 g          576 t
ORIGIN      
        1 atctgcggca aaggcagcca gcgaatcgta ttgtgcatcg gctaaatggg catacaatat
       61 tttttcggtt aaagtaagcg gcaaatgcct gcgtttgcgt aatgcttcaa ttttttgagg
      121 tagttcagca taaacctttt taatcatttc aatatcgaat gccataattg tttggggttt
      181 taaaatttgt aatattacag gtaatacgat gtgttggttt tacttaaatc aatttaaaaa
      241 acatttgatt ttagcgggcg caagttatta tttctgccca aaataatttt acacaacata
      301 ccttagtttt aaaactacct gttatttaat agttacaaaa atttaaatct tatcaaatca
      361 atcatgaaaa acggctgcat acttttaatt atattttgca ctgtgcaagt gcaagcacaa
      421 gtaaccgaca gcttgcaaaa acttataaac gggccgggca gcaatcaaca aaaacaagca
      481 tggtatcatg ccataaccgt ttttaaaagt aaaaccgatg cacaaggcgc attacaagct
      541 gcacaaaacg aatacaactt tgcattaaaa aataaaatgc ctttagcgca ggttgatgca
      601 cttttaatgt tttgccgtat ttatttacaa agtaaaaatt tagtgccggc tgatagtgtg
      661 ataactatgg ccgaaaagct ggtgcttcaa accaaggaca cgcaaatgcg ggcacaggtt
      721 attttatata aagccgatat tcaatcggac agaaatgaag tggctgaggc cattcagaac
      781 tatacgaaag ctttcgaatt gtttgaaaag aaattgtttt acaaaggcat ggccgctgca
      841 ataaaccgta tggcattgct cgaatttaaa cagggcaatt ttgaatcggc atttaagaaa
      901 gaccagttaa gtttttcgta ttcacaaatg acaaacgaca gtatgcttat gagtaatgcc
      961 gttagtaaca tgggcaatta cttttatatg aagcaacaat atgacagtgc gctttattat
     1021 tacaaccgta ccttgttgta tatgaataaa aatgcaacag gcagaattgc tatggtaaaa
     1081 agtaatgtag gcgatgtgct ggttgaactt cagaaattta aggaagcttt accctatttt
     1141 attgaagcca aagacgcttt tgaaaaggct ggtatggtac aacaactttc gcatttatat
     1201 acatctttag gtaggtgtta tgtgggatta aaaaattaca acgaagccga aacagcattc
     1261 aatcaatcgc tggcgcttga ggaagaaaac aaggacttta gtttaatagc agaaaactat
     1321 ggacacttaa gtgatttagc ggccttgctg ggtaattata aaaaagcctg ggagtatcag
     1381 cttaaagttg aacatgccaa agacagctta aaccaacaaa tggttaatga tgaagtgctt
     1441 gaattacaaa cacgtttcga aaccttgcag cgcgaaaaag ataatgcccg tttaaaagcc
     1501 gaaaatgccg tacatcaggc cgaaacccag cgccaacaag tattaattat tgcacttatt
     1561 gttagtgtag tattattggc tttaatagga ctaatgttgt ttttgattag caggcagcgg
     1621 caaaaaaacc ttgcagcgca aaaaatactt aacgatgcat tgacaaaaag taaaaaggat
     1681 gtagagcgct taaataatat gttggaaata cgtgccatac gcgcacaaat ggatccacac
     1741 tttatttata actgcatggc atcggcatta agtttactca atgcaaaccg tttagccgat
     1801 actgaaaagt atttagtaaa attttcgcgc ctgttgcgta tggtgttaga aaatgccgaa
     1861 tacaatgcaa taagtttaga caaagaaatt gaaatgctta ccatgtattt agatttagaa
     1921 agcatacgcc tcaatgccaa ttttacctat cagataaatg tggatgaaag cttgttggaa
     1981 gaaaacattg aagt
//
LOCUS       DAOMAA010000380         1989 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_57178_length_1989_cov_2.994312, whole genome shotgun sequence.
ACCESSION   DAOMAA010000380 DAOMAA010000000
VERSION     DAOMAA010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57178_length_1989_cov_2.994312"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /gene="serA"
                     /locus_tag="PLO59_05815"
     CDS             <1..978
                     /gene="serA"
                     /locus_tag="PLO59_05815"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HQV00657.1"
                     /translation="GTNQVDMTQAMLQGMAVFNSPYSNTRSVAELVIGECIMLMRKIF
                     EKNAAAHQGGWDKSSAGCFEVRGKTLGIIGYGHIGSQVSVLAEAIGMKVIFYDILPKM
                     ALGNAYAVKNLDELLAKSDMVTLHVPQNNGTKNLINDERIKKMKKGAVLINCARGNVV
                     EIEAVKKALQKQKLAGYAADVFPEEPQSNSDTFKLSLQGMHNVILTPHIGGSTVEAQE
                     NIGIDVATKMLAFLEGGSSVGSLTVPELSLPIQNNTHRLLHIHQNVPGVLSEVNSFLS
                     QHNVNILGQYLKTNERIGYVVLDIDKKTSGKALEALRHVKATIKVRSLY"
     gene            complement(990..1715)
                     /locus_tag="PLO59_05820"
     CDS             complement(990..1715)
                     /locus_tag="PLO59_05820"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HQV00658.1"
                     /translation="MAKLSVNINKIATLRNARGGNVPNVLQAALDIERFGADGITVHP
                     RPDERHIRFTDVWQLKQHLTTEFNIEGYPGTDFIKMVLDVKPHQVTLVPDAPDVLTSN
                     AGWDTITHQTFLTETVCQLKEAGMRVSLFIDTNLTLIEAAKTTGTDRIELYTEAYASG
                     FETQPVQAIAPYISAATMAQNMGLAINAGHDLNLQNLHYFAQQLPMLQEVSIGHALIS
                     DALYLGLDNVVKMYKHQLTKAIY"
BASE COUNT          622 a          351 c          419 g          597 t
ORIGIN      
        1 ggcaccaatc aggttgatat gacgcaggct atgttacagg gcatggccgt gtttaactcc
       61 ccttattcaa atacgcgctc tgttgccgag ttggttattg gagagtgtat tatgctgatg
      121 cggaaaatat ttgagaaaaa tgcagcagcg catcagggag gttgggataa atctagtgcc
      181 ggttgttttg aagtgcgtgg taaaacactg ggcattatcg ggtatggcca tatcggatcg
      241 caggtaagtg tgcttgccga agctattggc atgaaagtga ttttttatga tatactgcct
      301 aaaatggctt taggcaatgc ttatgctgta aaaaatttag atgagttgct tgctaaatcc
      361 gatatggtta cgctgcatgt accacaaaac aatggcacta aaaatttgat aaatgatgaa
      421 agaataaaga aaatgaaaaa gggtgcagtt cttattaact gtgcgcgggg taacgtagta
      481 gaaattgaag cagtaaaaaa ggcattacaa aaacaaaaac tggcaggtta tgctgccgat
      541 gtatttcccg aagaaccaca aagtaatagc gatacattta aactatcgtt acaaggtatg
      601 cacaatgtaa tacttacacc acacattggc ggcagtacgg ttgaagcaca ggaaaatatt
      661 ggtattgatg tggctaccaa aatgctggct tttttagaag gtggcagcag tgtaggttcg
      721 ttaacggtac ccgagttaag cttacccatt caaaataata cgcacaggct tttgcatata
      781 catcaaaatg tgcccggtgt attatcagag gtaaatagtt ttttatcgca acacaacgta
      841 aatattttag gtcaatacct taaaaccaat gaacgtattg gttatgtggt actggatatt
      901 gataaaaaaa cttcgggcaa agcattggaa gctttacgcc atgtaaaagc caccattaaa
      961 gtacgtagtt tatattagtt taatattact tagtatatgg ctttggttaa ctgatgctta
     1021 tacattttta caacattatc taaacctaaa taaagcgcat cactaattaa tgcatgccca
     1081 attgaaactt cctgtaacat gggtaattgt tgtgcaaagt agtgaaggtt ttgcaggttt
     1141 aaatcgtgcc cggcattaat agcaagcccc atgttttgcg ccatagtagc agccgaaatg
     1201 tatggcgcaa tggcttgcac aggttgtgtt tcaaagccac tggcataagc ttcggtataa
     1261 agctcaattc tatcagtgcc tgttgtttta gcggcttcaa ttaaagttaa atttgtatca
     1321 ataaataaag aaacccgcat acctgcttcc tttaattggc acactgtttc ggttaaaaat
     1381 gtttgatgtg ttatggtatc ccatccggca ttgctggtaa gcacatccgg tgcatcgggc
     1441 accaaagtaa cctgatgtgg ttttacatct aaaaccattt ttataaaatc ggtaccgggg
     1501 tagccttcaa tattaaattc agttgtaaga tgttgtttta attgccatac atcggtaaag
     1561 cgtatgtggc gttcgtcagg gcgtgggtgt acggtaatgc catcggcacc aaaacgttca
     1621 atatccaaag cagcttgaag cacattgggc acattgccgc cacgggcatt acgtaaggtg
     1681 gcaatcttat taatgtttac tgatagttta gccataatca ttttgtaatt gcatggcaac
     1741 attaccacac tgcacaaact tacttactta tatttacgtt gttaacgtat taaaacttac
     1801 ttaacatgca tgttttaata aattcaaacg cttcttgcat ttgtaaaaat gtatgtttac
     1861 cccgtttaaa attacagcaa taaattatgc aggcaggcca attaattaaa aataatatcc
     1921 caccgttaat accatccgat accgtaagca aggcattgca atggatggat ttttttaaag
     1981 tggatatgc
//
LOCUS       DAOMAA010000381         1989 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_57197_length_1989_cov_2.285936, whole genome shotgun sequence.
ACCESSION   DAOMAA010000381 DAOMAA010000000
VERSION     DAOMAA010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57197_length_1989_cov_2.285936"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..1266
                     /locus_tag="PLO59_05825"
     CDS             148..1266
                     /locus_tag="PLO59_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00659.1"
                     /translation="MLRVYSIILFVFASEIAIGQNKGTKKIVDTDVVICADLSASTNG
                     LITDLKENFWQLINHHATLTPQTNLRISYIFYGRPSFGSKSSFVKIATPLSNAYDSIF
                     NVIDDLKPNIEKGDQFVEAALDQAINHINWSTNKQALKCVFIIGNGSIMPGPGLEKLC
                     QQANQKHITINTIYCNSSLTEKELKWWEKLAYFGNGKSAAIHINKVLPSDGATNIKSL
                     YKLNNSLNQTIIGYGINGNTNHKLMQSIDSASLQNNASTFAARLIYKTAPANNCLPWD
                     LTCISSLPNFNLFELDRENLPEQFTNTSGNEMLEILKNNREQRQLILTQINELLEKDL
                     VKIKTNYVVQEIVGEEGNLNRVIIKQFVKQAIAHGSSW"
     gene            1301..>1989
                     /locus_tag="PLO59_05830"
     CDS             1301..>1989
                     /locus_tag="PLO59_05830"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HQV00660.1"
                     /translation="MLSSLYDNANLIFIGMVTSMMLYNAIKFLQLRNKTYLYYMAYML
                     FIIIYFSRSFLIHQGLLNSKEHTFIFSTIVPMMAYFCYYRFAILFLELNKTMRKLEFF
                     FKAMQLGVLAFIVLFLLCVFVFDNFELGIILHDVTRFVLIGASIYAITYTYQKKQKLT
                     NYFATGSLFLTLGGATAFTLSTLQYNGSLPNTFLASPLLYFMFGIIVEFLCFSLGLSY
                     KEFIRDAAKRNA"
BASE COUNT          673 a          321 c          338 g          657 t
ORIGIN      
        1 tttccttcga atccatggtt gcaaatttat tcaagttttt aaaaagtgta attaataagc
       61 gctttattaa acaaacagca ttgcaacaaa ttattggaag agtagaaata aatatattgt
      121 gcctgtattt agttgtttgt gtaataaatg cttagagttt attcgataat actttttgtt
      181 tttgcaagcg aaatagctat tgggcaaaac aaagggacga agaaaattgt tgacaccgat
      241 gtggttattt gtgccgattt aagcgcaagt accaacggat taataaccga tttaaaagaa
      301 aatttttggc aactgattaa ccaccatgcc acattaacgc ctcaaactaa tctgcgtatc
      361 agctatatct tttacggccg gccaagtttt ggcagcaaaa gcagtttcgt aaaaattgca
      421 acgcctttat ccaatgctta cgattcaatt tttaatgtga ttgatgattt gaaacctaat
      481 atagaaaaag gcgatcaatt tgtagaagca gctttagacc aagcaataaa ccacattaat
      541 tggagtacaa ataagcaagc gcttaaatgt gtttttatta tcggcaacgg cagtattatg
      601 cccgggccgg gtttagaaaa actttgccag caagccaatc aaaagcatat aacaatcaat
      661 accatttatt gtaatagcag cttaactgaa aaggaactta aatggtggga aaaattagct
      721 tatttcggaa acggtaagtc ggcagctatt catatcaata aagtattgcc atcggatggt
      781 gcaactaata tcaaaagcct ttacaaactc aacaacagcc tcaaccaaac cattattggt
      841 tatggcatta atggaaatac aaaccataag cttatgcaaa gtattgatag cgcttcatta
      901 caaaacaatg cttcaacttt tgcagcacgc cttatttaca aaactgcacc tgcaaataat
      961 tgtttaccat gggatttaac ttgtatatca tcattgccta attttaacct atttgaattg
     1021 gatagagaaa atttgcctga acaatttacc aatacatcag gtaacgaaat gcttgaaata
     1081 cttaaaaaca acagagagca acgtcaactt atattaacac aaattaatga attgcttgaa
     1141 aaggatttag tgaaaattaa aaccaattat gtagtgcagg aaattgtagg cgaggaagga
     1201 aacttaaacc gtgttattat aaagcaattt gtaaaacaag caattgccca tggtagtagt
     1261 tggtaaaaac ttttcgtagt ataaatatcc aataccatta atgcttagta gtttatacga
     1321 taatgcaaat ttgattttta ttggtatggt taccagtatg atgctgtata atgccattaa
     1381 gtttttgcaa ttgcgcaata aaacatactt gtattatatg gcgtatatgc tgtttataat
     1441 aatttacttt tcgcgtagtt tcttaatcca ccaaggctta cttaattcaa aagaacatac
     1501 ttttattttc agcaccattg tacccatgat ggcgtatttt tgctattaca gatttgcaat
     1561 cttatttctg gaattaaata agacgatgcg aaaattggag ttttttttta aagcaatgca
     1621 attgggtgta cttgcattta ttgttttgtt tttgctctgt gttttcgtgt tcgataattt
     1681 tgaattgggc attatactac atgatgttac gcgctttgta ctaattgggg catccattta
     1741 tgccattaca tatacatatc agaaaaagca aaagcttacc aattattttg caacgggctc
     1801 cttatttcta accttgggtg gtgccacagc ttttacacta agcacattac aatacaatgg
     1861 tagtttacca aatacttttc tggcatcgcc attgctatat tttatgttcg gaataatagt
     1921 tgaattttta tgcttttcat tgggtttatc ttacaaagaa tttatacgtg atgctgcaaa
     1981 aagaaatgc
//
LOCUS       DAOMAA010000382         1988 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_57230_length_1988_cov_3.124159, whole genome shotgun sequence.
ACCESSION   DAOMAA010000382 DAOMAA010000000
VERSION     DAOMAA010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57230_length_1988_cov_3.124159"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="PLO59_05835"
     CDS             <1..945
                     /locus_tag="PLO59_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HQV00661.1"
                     /translation="KRDANGFYKIDKILKGANWNKQQRSPLTEMGVDAKEGDYIIAVN
                     GTPTNQMNDIYASLMNTAGKQVELSLNSKPEKAGARKVIVVPIDNEAALYYYNWVQHN
                     IDYVNQATQGKVGYLHIPNMGVEGLNEFVKHFYPQQTKSALIIDDRGNGGGNVSPMII
                     ERLNRELAMVNISRNVAVSPNPGDMVVGPKVVLIDQYSASDGDIFPYRFKKYKMGKVI
                     GKRSWGGVVGIRGSLPFVDGGSLSKPEFSRYDTEGKEWIMEGYGVDPDIEIDNDPALE
                     YNGKDQQLDKAIEVILQELKSNPATLPKQPPYPKKNKQ"
     gene            complement(1043..>1988)
                     /locus_tag="PLO59_05840"
     CDS             complement(1043..>1988)
                     /locus_tag="PLO59_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00662.1"
                     /translation="FCSVLFTACTGYKKSHQALLHDKSYCNKQQLIAYTAADLPKPFY
                     TLQPDTLLTSRFSAQSLNMANAIGITALLTTYTRQCIDSNTHLNINKRLNLIEQLLHI
                     NHRINLASLEVSAIAGELDCEEERANQFAAYLKEKEAKTENKLIISSIILGAAGAISA
                     EIISNNSSNANFVTGFIIGVSLAEASLGALMLVNKKQIEFIHKDNALTDIRTHATVSN
                     YFPPAIWYYLNYENTQKGEKSLAKLLVEKWQLFGQVSENIESADHALFELYFGKGGTY
                     NADQLQNRADMLDQTQAYVTLMKQDLKNLAYEIELLKK"
BASE COUNT          602 a          345 c          441 g          600 t
ORIGIN      
        1 aaacgcgatg ccaatggttt ttataaaatt gataaaatac ttaaaggcgc taactggaat
       61 aaacagcaac gctcgccact taccgaaatg ggtgttgatg ctaaagaagg cgattatata
      121 attgctgtaa acggaacgcc caccaatcag atgaatgata tttatgcatc gttaatgaat
      181 acagcaggta agcaggtaga attaagcctt aacagtaagc ctgaaaaagc aggtgcgcgc
      241 aaagttattg ttgtaccaat tgacaacgaa gcagcacttt attattacaa ctgggtgcaa
      301 cacaatattg attacgttaa ccaggcaacg caaggcaagg ttggatacct gcacataccc
      361 aatatgggtg ttgagggact taatgaattt gtaaaacatt tttatccgca gcaaaccaaa
      421 agtgctttga ttattgacga ccgcggtaat ggtggtggca atgtatcgcc catgattatt
      481 gaacgtttaa accgtgagtt ggctatggta aatatatcgc gtaatgtggc cgtatcgcca
      541 aatccgggcg atatggtagt aggccccaag gtggtgctta ttgaccaata cagcgcaagc
      601 gatggtgata tatttcccta tcgttttaag aaatacaaaa tgggtaaagt tatcggaaaa
      661 agaagttggg gtggggtagt aggtataagg gggtcgttac cgtttgtaga tggcggtagt
      721 ttgagtaaac ctgagttttc gcgttatgat acagaaggca aagagtggat aatggaagga
      781 tatggtgtgg accctgacat tgaaatagac aatgaccccg ctttggagta taatggtaaa
      841 gaccagcaat tagataaagc aattgaggtg atacttcagg aactaaaaag caatccggct
      901 acgttaccaa aacagccgcc ttatcctaag aaaaacaaac agtaacagtt actataaatg
      961 tataatgtta gtcgttacta acacgtgcta cgcaatgctt gagggtatgt tgggtaaata
     1021 tgaattaaat ttaacagcag ctttattttt taagcaattc aatttcataa gccagatttt
     1081 ttaaatcttg tttcatcagt gtaacataag cctgggtttg atctaacatg tcggcccggt
     1141 tttgtaattg gtcggcatta taggtgccgc ctttgccaaa gtataactca aataatgcat
     1201 ggtcagcact ttctatattt tcagaaacct gaccaaatag ctgccatttt tctaccaaca
     1261 gtttggctaa cgatttttca cctttttggg tgttttcata gttgagataa taccaaatag
     1321 ctggaggaaa ataatttgac acggttgcat gggtccttat gtcggttagc gcattgtctt
     1381 tatgaataaa ttcaatttgc tttttattaa ccaacatcaa tgcgcctaac gatgcttcgg
     1441 ctaagctaac cccaattata aagccggtca caaaatttgc gttcgaactg ttgttcgaaa
     1501 ttatttcggc tgatatggca ccagctgcac caagaataat agaactgatt atgagtttgt
     1561 tttcggtttt agcttctttt tcttttaaat acgcagcaaa ttgattggca cgttcttctt
     1621 cacaatcaag ctcgcctgca atggccgata cttcgagcga tgccaggttt attctgtgat
     1681 taatatgcag cagttgttct attaagttaa ggcgcttatt tatgtttaga tgtgtgttgc
     1741 tatcaataca ttggcgtgtg tatgttgtta gtaaagcggt aatgccaatg gcatttgcca
     1801 tatttagcga ctgtgccgaa aacctgcttg ttaatagagt gtcgggttgt aaggtgtaaa
     1861 aaggctttgg caaatcggca gcggtgtaag caattagttg ttgcttgttg caataacttt
     1921 tatcgtgcaa taatgcctga tgcgattttt tatatcccgt acatgcggta aacaaaacgc
     1981 tgcaaaat
//
LOCUS       DAOMAA010000383         1987 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_57288_length_1987_cov_3.557453, whole genome shotgun sequence.
ACCESSION   DAOMAA010000383 DAOMAA010000000
VERSION     DAOMAA010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57288_length_1987_cov_3.557453"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="PLO59_05845"
     CDS             complement(<1..206)
                     /locus_tag="PLO59_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00663.1"
                     /translation="MANKIDILENNWIEQVFKDRNKSYGAYFLRKIYSHHITRGAIIS
                     LAIFTIGASAPKIYDWITGMTGAF"
     gene            complement(212..841)
                     /locus_tag="PLO59_05850"
     CDS             complement(212..841)
                     /locus_tag="PLO59_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HQV00664.1"
                     /translation="MASIDTGGSDGKEGGKVRAKKMAVHIDFTPMVDLGFLLITFFML
                     TTTMNKPQVMEINMPLKEDVPPEDRTKFKESQTMTIVLGENNKVYYYFGITNPVLDST
                     NFSNDGIRKVLLEENKKRNPLVDSIAIYKRMLDNRIIKREEYKKNSARIKAYKDALIV
                     VIKAEDKSKYRNLVDILDEMAICNIGRYAIVDITEQDKQLIAGTLPTTN"
     gene            complement(926..1522)
                     /locus_tag="PLO59_05855"
     CDS             complement(926..1522)
                     /locus_tag="PLO59_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HQV00665.1"
                     /translation="MPKVKVKRKPPHIDMTAMTDVAFLLLTFFMLTTKFKPDEPFVVD
                     PPSSVSDYKLPESNVIQISVDATGKVFFSTGQGDRRTTLEKMAKQYNLTFTEQELRSF
                     SLLETFGVPVSQLKQYLALKPNERAKVNQPGIPTDSTDNQLRDWILFARQSNPQAIIA
                     IKGDGDAEYPVMKNIIATLQERKVNRFNFITNLENAPQ"
     gene            complement(1543..>1987)
                     /locus_tag="PLO59_05860"
     CDS             complement(1543..>1987)
                     /locus_tag="PLO59_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HQV00666.1"
                     /translation="SGSLGNVVKSAMIKYKEVQTEQHMDKDQKIVAIQKQIEEATELE
                     MPMLQKNLVFLSTIATVATLIALLGTVLGMIKAFASLAQAGSPDSVGLATGISEALIN
                     TAFGIGTSALAVISYNYFTTKIDSLTYAIDEAGFSIVNTFASTQK"
BASE COUNT          541 a          355 c          401 g          690 t
ORIGIN      
        1 ttaaacgccc cagtcatacc ggtaatccag tcataaattt taggtgcgct tgcgccaatt
       61 gtaaatatgg ctaagctgat aatagcaccg cgtgtaatat ggtggctgta tatttttctt
      121 aaaaaatagg caccatagga tttgttgcgg tctttaaaaa cctgctcaat ccagttgttt
      181 tctaggatat caattttatt tgccatggtt tttagtttgt tgtgggtaag gtacctgcaa
      241 taagttgttt gtcctgttct gttatgtcaa ctatggcata acgtcctatg ttgcaaattg
      301 ccatctcgtc aagtatatca accaagttgc gatattttga tttgtcttcg gctttaatta
      361 caacaatgag tgcatctttg tacgctttaa tgcgtgctga atttttctta tactcttcgc
      421 gcttaataat gcggttgtcg agcatgcgtt tgtaaatggc aatagagtca accaaagggt
      481 tacgtttttt attttcctca agtaaaactt ttctgatgcc atcgttacta aagttggtcg
      541 agtctaacac tgggttagta ataccaaagt agtaatacac cttattgttt tcgccaagta
      601 caatcgtcat ggtttgcgat tctttaaact tggttctgtc ttcgggtggt acatcttctt
      661 taagaggcat gttaatttcc attacctgag gtttgttcat agtagtggtt aacatgaaaa
      721 aggtgatgag cagaaatccc aagtcaacca tgggagtaaa gtcaatatgt actgccattt
      781 ttttggcccg tactttacca ccttctttac catcgctacc gcctgtatca attgaagcca
      841 tgttttattt ttttgtgggc tattgccgct ttatgtttag ttgatgataa ttggtttatg
      901 attccataaa ccgattgaaa aaaaattatt gtggtgcgtt ttcaagattg gtaataaagt
      961 tgaagcgatt tactttacgt tcttgaagtg ttgctatgat gtttttcata acaggatatt
     1021 ctgcatctcc atcaccttta attgcaataa tagcttgtgg attgctttgt cgtgcaaata
     1081 aaatccaatc gcgaagctga ttatcagttg agtcggtagg gataccgggc tggtttactt
     1141 ttgcgcgttc attgggcttt aaggcaaggt attgtttcaa ttgacttacc ggtacaccaa
     1201 aagtttctaa caaagaaaaa ctacgtaatt cttgctcggt aaacgtaagg ttgtattgct
     1261 tggccatttt ttcaagtgta gttctgcggt cgccttggcc tgtactaaaa aacactttgc
     1321 ctgttgcatc aacgctaatc tgaataacgt ttgattcggg caatttgtaa tcagataccg
     1381 atgatggcgg atcaacaacg aaaggttcgt caggcttaaa cttggtggta agcataaaaa
     1441 aggtaagcaa cagaaatgca acatcggtca tggctgtcat gtcaatgtgc ggtggtttcc
     1501 gttttacttt aacttttggc attcgcttta attattacaa agttattttt gagttgaagc
     1561 aaaagtgttt acgatactaa agccagcttc gtcaattgca tatgtaagcg agtcgatttt
     1621 agttgtaaag taattgtatg aaataacagc caaagccgat gtaccaatac caaaagcggt
     1681 attaataagt gcttcagaaa taccggttgc taaacctacc gaatcgggcg agcctgcttg
     1741 tgctaacgag gcaaaggctt taatcatacc caatacagta cctaaaagtg caataagtgt
     1801 agctactgta gcaatggttg atagaaacac caagttcttt tgcaacatgg gcatttcaag
     1861 ttctgttgct tcttcaattt gtttttgaat agctacaatt ttctggtctt tgtccatgtg
     1921 ctgttcggtt tgtacttctt tatatttaat catggcactt ttaaccacat tacctaatga
     1981 acctgat
//
LOCUS       DAOMAA010000384         1981 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_57687_length_1981_cov_2.368640, whole genome shotgun sequence.
ACCESSION   DAOMAA010000384 DAOMAA010000000
VERSION     DAOMAA010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57687_length_1981_cov_2.368640"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1454
                     /locus_tag="PLO59_05865"
     CDS             <1..1454
                     /locus_tag="PLO59_05865"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HQV00667.1"
                     /translation="PLDISPLNLGSQINTVADEYYPSLTIDNQTLVFTRNTIDEGSRM
                     PQEDFFVSTQKNKQWQAAHQVGSPLNTDLNEGAPSISADGKLLFFAACDRRDGMGSCD
                     IYFSRWVNNSWSTPINLGSPINTNAWETQPSFSADGKTLYFIRGYHTHEGIKQQDIYV
                     TSIGSNGNFENPVKLSDSINTTEAEESVFIHPDNQTIYFSSRGHIGMGGLDIFLSRKN
                     ANGQWGKAQNLGYPINTAGDENSLLISPQGDKAYFATNRTGGFGGLDLYSFDLPATLK
                     PQPITYVKCAITDGANGLPLAASFEIIDVETQEVVYADKVNKTGAFTRSLTVGKDYLL
                     NVTQPGYLFYSDNFSCKKAVDYNNPYELKIGLQKIEKGSKVILRNVFFDTDSATLKTA
                     SYVELKKLQIFLQQNQQVNITITGYTDNTGNTVKNQKLSENRAHAVYMHLISMGIDAK
                     RLQYIGMGDKQPIADNNTDAGRALNRRTEAEIR"
     gene            1541..>1981
                     /locus_tag="PLO59_05870"
     CDS             1541..>1981
                     /locus_tag="PLO59_05870"
                     /inference="COORDINATES: protein motif:HMM:NF019737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family peptidase propeptide
                     domain-containing protein"
                     /protein_id="HQV00668.1"
                     /translation="MQRFFVFIFLFSLTQAWAQLDNLPLQWHNKNGINTISVATDYQI
                     QTITTDSGTKHILHADQANACLQKGMPSLPYFAAPLLMPDVKGLQALIIDSTFIVLNN
                     IDLLPSKGDISRNINPDEVAYTFGKEFNTDAFYPQNILQHQEVFR"
BASE COUNT          666 a          361 c          369 g          585 t
ORIGIN      
        1 cgccattgga tataagtccg ttaaatttag gttcgcaaat taacaccgtt gccgatgagt
       61 attacccatc gctaaccatt gataatcaaa cgttggtttt tacgcgcaat accattgatg
      121 agggcagtcg tatgccacag gaagattttt ttgtatccac gcaaaaaaat aagcaatggc
      181 aagcagcaca tcaggtgggc agcccattaa acacggatct taatgaaggt gcaccaagta
      241 tttcggccga tggaaagctt ttgttttttg cagcatgcga tagacgcgat ggcatgggta
      301 gctgtgatat ttattttagc agatgggtaa ataacagttg gagtacgccc atcaatttag
      361 gttcgccaat aaataccaat gcctgggaaa cacaacccag ttttagcgct gatggcaaaa
      421 cactttattt catcagaggc tatcatacac atgaaggtat taaacagcag gatatttatg
      481 ttacctcaat tggaagcaat ggtaattttg aaaatccggt taaattatca gatagtataa
      541 atacaactga agccgaagaa agtgtgttta tccatcccga taatcaaacc atttattttt
      601 catctcgtgg ccatataggt atgggtggtt tagatatttt tttaagtcgt aaaaatgcaa
      661 acggtcaatg gggtaaagca caaaatttag gctatcccat taatacagcg ggcgatgaaa
      721 atagtttgct cataagtccg caaggcgata aagcatattt tgccaccaac agaacgggcg
      781 gttttggagg gttggattta tatagtttcg atttgcctgc aacacttaaa ccacagccga
      841 taacatacgt taaatgtgct ataactgatg gtgccaatgg cttaccgtta gctgcatcat
      901 tcgaaattat tgatgttgaa acccaagaag tagtgtatgc tgataaagta aataaaacag
      961 gcgcatttac acgaagcctt accgtgggta aagattattt acttaatgta acacagccgg
     1021 gttatctgtt ttattctgat aatttttcat gcaaaaaagc cgtagattac aacaaccctt
     1081 atgaacttaa aattggctta caaaaaattg aaaaaggcag taaagtaatt ttgcgaaatg
     1141 tgtttttcga taccgatagt gctacattaa aaacggcatc ttatgttgaa ttaaaaaaac
     1201 tgcaaatatt tttacaacaa aatcaacaag ttaacataac cattaccggc tatactgata
     1261 acaccggaaa tactgtcaaa aatcaaaaac tttccgaaaa tcgtgcccat gcagtttata
     1321 tgcatttaat ttcaatgggt attgatgcca agcgcttgca atatattggt atgggcgata
     1381 aacagcccat tgccgataac aataccgatg ccggccgtgc ccttaatcgc agaacagaag
     1441 ccgaaatacg ataaccctaa aatttacata aatgataaag tgtaaataca aaatgtattt
     1501 taatagtaag tcaataactt acttttgact tttattttcc atgcaacggt tttttgtttt
     1561 tatttttttg ttttcgctta cgcaagcttg ggcgcaactt gataatttgc cgttgcaatg
     1621 gcataacaaa aatggaatta ataccatttc agtagctact gattatcaaa tacaaaccat
     1681 aacaacagac agtggcacca agcatatttt acatgcagat caggcaaatg catgcttgca
     1741 aaaaggcatg ccttcattac catatttcgc agcaccatta ttaatgcccg atgtaaaagg
     1801 tttacaagct ttaattattg attcaacttt tattgtctta aacaatattg atttgttacc
     1861 ttcaaaaggc gatataagca gaaatatcaa ccctgatgaa gttgcataca catttggtaa
     1921 ggaatttaac acagatgcgt tttaccccca aaatatattg cagcatcagg aggttttcag
     1981 g
//
LOCUS       DAOMAA010000385         1978 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_57878_length_1978_cov_2.384815, whole genome shotgun sequence.
ACCESSION   DAOMAA010000385 DAOMAA010000000
VERSION     DAOMAA010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57878_length_1978_cov_2.384815"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="PLO59_05875"
     CDS             complement(<1..361)
                     /locus_tag="PLO59_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014782578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQV00669.1"
                     /translation="MISVNNVSKQYDKQWALSDVSFQVTKGEIVGFLGPNGAGKSTMM
                     KIITCYLPATSGIVKVEGYDTLEQSIEVRKQVGYLPEHNPLYLDMYVREYLSFVAGIH
                     LSKNLIAKAVTNMIELTG"
     gene            complement(393..1145)
                     /locus_tag="PLO59_05880"
     CDS             complement(393..1145)
                     /locus_tag="PLO59_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002701329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HQV00670.1"
                     /translation="MKLCDKLNWSGETASASKSREYKDGKSKLTQLIAQSDFRGKDIL
                     IIDDNSKDGTAEIVKKLQVEFDGRLHLEQRAGKLGLGTAYIHGFKWALQRNYEYVFEM
                     DADFSHRPKDLIKLLEACENGADVAIGSRYKTGVNVVNWPIGRVIMSYYASAYVRLIT
                     RMPIHDATAGFKCYKSKVLKAINFDRIKFVGYAFQIEMKFTAWKLGFVIAEVPIVFTD
                     RKHGKSKMSGGIFSEAFFGVIKLKVRSWFIKY"
     gene            complement(1616..>1978)
                     /locus_tag="PLO59_05885"
     CDS             complement(1616..>1978)
                     /locus_tag="PLO59_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00671.1"
                     /translation="VLGVGSYTYQMNTRDTMGFAAKGAWFEVSQSAWSHPDEGELTIK
                     TGYNIYKDPVTDDGSKKSLKGFQFVQFNNGEFEVISEATEEVAYSDANILQTIYKDGK
                     FYNETTLTEIRERMDKLV"
BASE COUNT          604 a          378 c          287 g          709 t
ORIGIN      
        1 agcctgttaa ctcaatcata ttagtaacgg ctttggctat taaatttttt gatagatgta
       61 tgcctgccac aaaactcaaa tactcgcgca catacatatc taaatagagc ggattgtgct
      121 cgggtaaata acctacctgt ttgcgcactt cgatagattg ctccagggtg tcgtaccctt
      181 ctaccttaac tattcctgat gttgcgggca ggtagcaggt aataattttc atcatggttg
      241 atttgcctgc tccattaggt cctaaaaaac caactatttc gcccttggta acctgaaacg
      301 aaacatcgct taaagcccat tgtttatcat attgtttcga aacattgttt actgaaatca
      361 tattgttatg caaacattat tattggtaag ccttaatatt taataaacca actgcgtact
      421 tttaatttga taacaccaaa aaatgcttcg ctaaaaatgc caccactcat tttggattta
      481 ccatgtttac gatcggtaaa aactataggc acttcggcaa ttacaaagcc cagtttccag
      541 gcagtaaatt tcatttcaat ctgaaaagca tagcccacaa attttatccg gtcgaaatta
      601 atagctttta aaactttgct tttgtaacac ttaaagcctg ctgttgcatc gtgtatgggc
      661 atgcgtgtta taagacgtac gtatgccgag gcatagtaac tcataataac gcgaccaatg
      721 ggccagttaa ccacatttac tccggttttg tatcgcgatc caatagctac atcggcaccg
      781 ttttcgcagg cttcgagtag cttaattaaa tcttttggcc ggtggctaaa atcggcatcc
      841 atttcaaaca catattcata atttcgctgc aacgcccatt taaaaccgtg tatataggcc
      901 gtacctaaac ccaatttgcc ggcacgttgt tctaaatgca aacggccatc aaattcaacc
      961 tgtagttttt ttacaatttc tgcagtgccg tctttgctgt tatcatcaat aattaaaata
     1021 tctttacctc taaagtcgga ttgcgctatt aattgggtta gtttagattt accatctttg
     1081 tattctcttg atttagaagc ggatgctgtt tctcctgacc aatttaactt atcacaaagt
     1141 ttcattaatg gtttaaaccc tcctgcatct gttgacatta aaattgtgtt tttgatatta
     1201 atgttattat tggcaacagg atttgcattt aaaagattta aaactcttaa tataaattca
     1261 ctattatcaa taatttttac atcatcaatt aatgcttcag tagcttcttg attatgtggg
     1321 tgaaatacag taacagaatt aaatttcatt tcattaatga atttacatac tagttttaaa
     1381 ttagcaggtt catcttcatt aaatcttcta tctgcttgtg catcaaataa atttggaata
     1441 gttaaattta ctttaacttt attatggtca caaacatctt ttatttgtct taattgccat
     1501 aaatcctcat atgaattaat acggaaagta agattatatt cagtttgatc tttaactaat
     1561 tcaactttag tataaaaact accatcagga tattttattt tttctatttt cataactata
     1621 ctagtttatc cattcgttca cgaatttctg ttaatgttgt ttcattataa aacttaccat
     1681 ctttataaat agtttgtaat atattagcgt ctgaataagc tacttcttct gttgcttcag
     1741 atatcacttc aaattctcca ttattaaatt gaacaaattg aaatcctttt aatgattttt
     1801 tagaaccatc gtctgtaact gggtctttgt aaatattata acctgtttta atagttaatt
     1861 caccttcatc aggatgagac catgctgatt gagaaacttc aaaccaagca cctttagcag
     1921 caaaccccat tgtgtctcta gtattcattt gatatgtata agaacctaca cctaacac
//
LOCUS       DAOMAA010000386         1975 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_58045_length_1975_cov_2.834896, whole genome shotgun sequence.
ACCESSION   DAOMAA010000386 DAOMAA010000000
VERSION     DAOMAA010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58045_length_1975_cov_2.834896"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1183)
                     /locus_tag="PLO59_05890"
     CDS             complement(<1..1183)
                     /locus_tag="PLO59_05890"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HQV00672.1"
                     /translation="MKFTFVYRQLFLFLFILIFKSNTNAQVTLPSGFSDKLVANGFNQ
                     IQGFVQDSIGRYYTWERGGRVIILDTNLTKLPTLIDIKEEVGSWGDHGLNAIALDPAF
                     ESNGYIYLFYTVDRHHLMNFGTANYNANTNQYYEATIARLTRYTCDEATNYTSIIPNS
                     RLVLIGSNVTNGIPVLASNHSGGGLAFGSDGSLLVATGDAAVNGGDTGSFVGTYWQQA
                     IADSILKPTNNVGAYRSQIVQSLNGKILRINPANGNGYQSNPFYNPNRPQSAISLVYA
                     LGFRNPFRISVKKGTGSTDVTAGDPGIIYIGDVGATKYEELNVCDASGQNFGWPLYEG
                     FSTTTEFYGKNHTNLYAANPLYGQGCNQPYFTYMELLKQPIITGLPIFKNTCDTNISI
                     PA"
     gene            complement(1447..>1975)
                     /locus_tag="PLO59_05895"
     CDS             complement(1447..>1975)
                     /locus_tag="PLO59_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HQV00673.1"
                     /translation="KPDDVSTDIDQLASALTSRFKEFCKQYGSELEIWFEPGKFLVSE
                     CGNLLVSVNVLKQTTSTVFVGVNSGQNHLIRPMFYDAYHHITNVSNPTGKPRLYSVVG
                     YICETDTFGWDRKIAEVHEGDILAIANAGAYGYAMSNNYNARLRPPEVLILNGKTTLI
                     RRRETLDDLLATQIS"
BASE COUNT          597 a          381 c          372 g          625 t
ORIGIN      
        1 cggctgggat ggaaatattg gtatcgcagg tgtttttaaa aataggtaaa ccagttataa
       61 ttggttgctt taataactcc atgtatgtaa aatatggttg gttgcaacct tgcccataca
      121 gtggattagc cgcataaagg ttggtgtggt ttttaccata aaattcagtt gttgtactaa
      181 agccttcata taatggccat ccaaaatttt gaccactagc gtcacataca tttagctctt
      241 catatttagt tgcgcctaca tcgccaatat aaattatgcc cggatcaccg gcagtaacat
      301 ctgtactccc tgtacctttc ttaacactta ttctgaatgg atttctaaaa cctaaagcat
      361 agacaagcga tatggccgat tgcggtctgt tcggattata aaatggattg ctttgatatc
      421 cgttgccatt ggctgggttg attcttaaaa tttttccatt taagctttgc acaatttgag
      481 atcggtaggc tcccacatta ttagtgggtt ttaaaattga atcggcaatt gcctgttgcc
      541 aataagtgcc cacaaagcta cccgtatcgc caccgtttac ggctgcatca ccggttgcaa
      601 ctaacaacga gccatcactg ccaaaagcaa gcccaccacc gctgtgatta gaagcaagca
      661 caggtatgcc attagtaaca ttgctaccaa ttaaaaccaa acggctgtta ggtataattg
      721 aagtgtagtt ggttgcctcg tcacacgtat agcgtgttaa ccgcgcaata gttgcttcat
      781 aatattgatt ggtgtttgca ttgtaattag ccgtaccaaa attcatcagg tgatgacggt
      841 caaccgtata aaacaaatag atatagccat tactttcaaa tgccggatct aatgcgatag
      901 cattcaatcc gtgatcgccc caagacccta cttcttcttt tatatcaatt aatgtaggaa
      961 gtttagtaag gttggtatct aatataatta ccctgcctcc gcgttcccat gtgtaatagc
     1021 gtccgatgct gtcttgtaca aaaccctgaa tctggttaaa accattggca accaatttat
     1081 cgctaaatcc gctgggaagc gttacctgtg cattggtgtt cgatttaaaa atcaggataa
     1141 ataagaagag gaatagttgt ctgtaaacaa aagtaaattt cacgatcatt atttttagtt
     1201 tgttggggga tgcaaacaca tatttgggcg aaacgccaaa aatcgtgggt gaaaatacaa
     1261 taaatggtgg ctaaagttac gttggcctta tttttttttt gaaatttatt aactacaacc
     1321 tattgaaaac ccgattagaa ttaatggatt tatccgtaaa tacttaatac ttagtgtatt
     1381 aactacaata agtaggagcg taacgttttc agaaattttt tagttttttt taaacggagg
     1441 aaaagattag ctgatttgtg ttgctaataa atcatctaaa gtttcgcgcc ttctaatcag
     1501 ggttgttttg ccgtttaaaa ttaaaacttc aggcgggcgc aaacgggcat tataattatt
     1561 tgacatagcg taaccgtagg ctccggcatt tgctattgct aatatatcac cttcatgtac
     1621 ttcggcaatt tttctatccc agccaaaagt atcggtttcg caaatataac ccacaacact
     1681 atataaacgt ggctttccgg tggggttgct tacattggtt atatggtggt aagcatcata
     1741 aaacataggt cgtattaagt gattttgacc ggagttaact ccaacaaaaa cggttgaagt
     1801 tgtttgtttg agaacattaa cactaacaag taaattgcca cattcactta ctaaaaattt
     1861 gcccggctca aaccaaattt ctaattcact accatattgc ttacaaaact ctttaaacct
     1921 tgaagttaaa gcacttgcaa gttggtcaat atctgtactt acatcatcag gcttg
//
LOCUS       DAOMAA010000387         1973 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_58162_length_1973_cov_2.746090, whole genome shotgun sequence.
ACCESSION   DAOMAA010000387 DAOMAA010000000
VERSION     DAOMAA010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58162_length_1973_cov_2.746090"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1078
                     /locus_tag="PLO59_05900"
     CDS             <1..1078
                     /locus_tag="PLO59_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQV00674.1"
                     /translation="KIDVINGVGKLLAGKKVSVTLDGAETIYEANHIILATGARSRQL
                     PNIKQDGVKVFGYREAMTLPKQPASLVVIGSGAIGSEFAYFYNAMGTKVTIVEFMPRI
                     VPVEDEEVSKQMERSFKKQGINIFTDSSVESVDTSGALCKVQIKTKKGMEVIEAESVL
                     SAAGVVANIENLGLEELGIVTDKGKVVVNDYSQTNMPGIYAIGDITKGQALAHVASAE
                     GILCVEKIAGLDVHPINYNNIPGCTYTTPEIASVGYTEAAAKEAGYELKIGKFPFSAS
                     GKAKAAGAADGFVKVIYDAKYGELLGAHLIGYNVTEMIAEIVVARNLETTGHEILKSI
                     HPHPTISEAIMEATAAAYGEVIHM"
     gene            complement(1137..>1973)
                     /locus_tag="PLO59_05905"
     CDS             complement(1137..>1973)
                     /locus_tag="PLO59_05905"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HQV00675.1"
                     /translation="DFGFIGMLLLPFNKILNKLKAVDNPIKFKSNSVNSSTAEFTYLD
                     QAFENLMLKIKDDFNHEKAFIANVSHELQTPISILQNRFENIITDQKTDHDTALKLID
                     SQKILSRLSRIIKALLTISKVENEQFDKSEVTNLNDLISEIIEELQDRCAHKEVTMIN
                     DMEHQIILAKSNTSLLQMLFNNLLINALKYNKHGGNVTITSKLINNDFEIAISDTGLG
                     IEPEQIAIIFDRFTRLHQKDANSHGLGLSIVKTIANYHQLNIAVTSTIGVGTTFIISG
                     KS"
BASE COUNT          620 a          323 c          410 g          620 t
ORIGIN      
        1 taaaatagat gtaattaatg gagttggtaa acttttggca ggcaaaaagg taagcgtaac
       61 cttagatggt gctgaaacca tttatgaagc caatcacatt attttagcaa ccggtgcacg
      121 ctcgcgccaa ttacctaata ttaagcaaga cggtgtgaag gtgtttggct accgcgaagc
      181 aatgacattg cccaagcagc ctgcttcatt ggtggtaatt ggtagtggcg caattggtag
      241 cgagtttgca tatttttata acgccatggg cacaaaggtt accattgtag agtttatgcc
      301 acgtattgtg cctgtagagg acgaagaagt atcgaaacaa atggaacgct cttttaaaaa
      361 acaaggcatt aatattttta ccgattcaag tgttgagtca gttgatacat cgggagcctt
      421 atgtaaggtg cagattaaaa ccaaaaaggg tatggaggtg attgaggccg aatctgtatt
      481 gagcgctgcc ggtgttgttg ccaatatcga aaacttaggt cttgaagaat tgggcattgt
      541 taccgacaaa ggcaaggtag tggtaaatga ttattctcaa accaatatgc ccggtattta
      601 tgcaattggc gatataacaa aaggccaggc attggcacac gttgcatcgg cagaaggtat
      661 tttatgtgtc gaaaaaattg ccggcttaga tgtgcatcct attaactaca acaatatacc
      721 cggttgtacc tataccacac ccgaaattgc atcagttggt tataccgaag ctgctgcaaa
      781 agaagcaggc tatgaattaa aaattggcaa attcccgttt agtgcatcgg gcaaagcaaa
      841 agcagcaggt gctgccgatg gttttgtaaa agtaatttac gatgctaaat atggcgaatt
      901 attgggcgcc catttaatag gatataatgt aaccgaaatg attgccgaaa tagtagtagc
      961 acgaaattta gaaactacag gccatgaaat attaaaatca attcatccgc acccaaccat
     1021 tagcgaagca ataatggagg ctactgctgc tgcttacggt gaggttattc acatgtaatt
     1081 gattaaaaaa taaatttaat taaaaactgc tttgcccaaa tggtaatgca gtttttttat
     1141 gatttaccgc taataataaa tgtggtacca acaccaatag tactcgttac agctatgttt
     1201 agttgatggt aattggcaat ggttttaaca atggacaaac ccaagccatg actattggca
     1261 tctttctgat gtaatctggt aaaacgatcg aatataatgg caatttgctc gggctcaata
     1321 cctaaaccag tgtcggaaat ggcaatttca aaatcattat taattaattt gctggtaatg
     1381 gttacgttgc ctccatgttt attgtatttg agggcattga taagcaaatt attaaaaagc
     1441 atttgtaaca atgaagtatt gctttttgct aaaataattt ggtgttccat atcatttatc
     1501 atggtaactt ctttatgtgc acaacgatct tgcagctctt caataatttc gctaataagg
     1561 tcgtttaaat tggtaacctc acttttgtca aactgttcgt tttctacttt tgatattgtt
     1621 agcaatgctt taataattcg acttaaccgg ctcaatattt tttgtgagtc aataagtttt
     1681 aaggcggtat cgtgatcggt tttttgatcg gtgattatat tttcgaaacg gttttgtaat
     1741 atcgatatgg gtgtttgtaa ttcgtgtgat acattggcaa taaatgcttt ttcatgatta
     1801 aagtcgtctt taattttaag cattaagttt tcgaaagcct gatcgagata tgtaaactca
     1861 gcagtagaac tattaaccga attcgatttg aattttatag ggttatcaac tgctttaagc
     1921 ttgtttagta ttttattaaa aggcaaaagc aacataccga taaagccaaa gtc
//
LOCUS       DAOMAA010000388         1972 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_58227_length_1972_cov_2.423579, whole genome shotgun sequence.
ACCESSION   DAOMAA010000388 DAOMAA010000000
VERSION     DAOMAA010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58227_length_1972_cov_2.423579"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1478)
                     /locus_tag="PLO59_05910"
                     /pseudo
     CDS             complement(1..1478)
                     /locus_tag="PLO59_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013621502.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
     gene            complement(1621..>1972)
                     /locus_tag="PLO59_05915"
     CDS             complement(1621..>1972)
                     /locus_tag="PLO59_05915"
                     /inference="COORDINATES: protein motif:HMM:NF013506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HQV00676.1"
                     /translation="VANGRNMPQSMVDSIGQGRVWSGKTALQLGLVDTLGGLNDAIKI
                     AADLAKIKTYRTYELPIQKEPFSELLEDLNGGVKTAIIKNELGPEFEIYHQVSKLIKQ
                     EGVFMRMPFELQLN"
BASE COUNT          574 a          386 c          350 g          662 t
ORIGIN      
        1 attattctgt tgttggaaag gcgtggcact ttgtgttggc cgcctaattt gtctttaagc
       61 tttaaccact tgtaaaatgt ttgagcgggc attgtgcgaa taattggcat acgtaacact
      121 ttgtcctgat agcgttttgc ttcgtaatcc gaatttagtt ctttaagggt attatcaagc
      181 acggttgcaa atacattcag gttttcaggt ggtttttcaa actcaataaa ccattcatgt
      241 gcgccacatt cgttttcgga aatataaacc ggtgctgccg tataatcagt aatcattgca
      301 ccggtgctta aacaggctgc ggcaatagct ttatcggcat tgtcaacaat aacttcttcg
      361 ccaaatgcat ttatataatg tgaagtgcgg cctgtaattt ttatgcgatg tggcgatagg
      421 gatgtaaatg ttacagtgtc gccaatttgg taacgccaca aaccgccatt agtggtaatg
      481 agcatcgaat agtttaagcc taaaaccact tctgataatg atatggcttg ttgcgaaaaa
      541 gcgccactgc tttcgggaat aaattcatag taaacaccat agtctaacat caatagcatt
      601 tcattgctgt tattcaaatc ctgtatgcca aaaaatcctt ctgatgcatt ataggtttcg
      661 aggtattgca tcttattacc ggggatgagt gcattaaact gacttcggta tggtgtaaaa
      721 cttactgcac catgaacaaa cagttctaaa tttggccata cttcatgtat gttttgtttg
      781 ccggtaattt gcaatatatg cctaagtaaa accaacatcc acgatggtac gccactaata
      841 ttggttacat cttctttaat ggtaatgtta gccatcttaa ttattttttc ttcccattcg
      901 tccattaaag ccacactcaa ttcaggtgta cggaagagtt gaatccaaaa aggtaaattt
      961 tgcataatga tggccgacaa atcgccataa aacgattcgt tactaaatgc atttatctga
     1021 tgcgaaccac ctaaggttaa aaacctgcca ctaaataaat ttgattgtgg attgttactg
     1081 tagtatatgc ttaataaatc tttgcctcct ttaaaatggc actcttccaa actttcttca
     1141 gtaacaggaa taaacttgct tttgtcgctg gttgtgcccg atgattttgc aaaccatttt
     1201 acatcggttg gccataaaat attttgttcg cccagcatca gccttttaat atatggctta
     1261 tgtgtttcgt agttttgtac ttgcatacgg ctgcggtagt cggcctcatt ttttatggaa
     1321 gcaaaatcct aataacgccc tatttcagtg ttttttgctg tacttaaaag tttacgcaaa
     1381 gtttcttcct gtacatcaaa aggatacttt ataaaaagtt cgatctgatg catgcgtttt
     1441 ttcattaacc atgcaaaaag tgagtttata atggccatta agtacttgct tgaatttgat
     1501 attgaagcaa gtgcaataaa aatattttta gcttattatg gtagcaaaac catgtaacca
     1561 tttataaaaa aacagcatca aatttttgct tacaaattta agatgccgca acccatacta
     1621 ttaattaagc tgcaattcaa aaggcatacg cataaacaca ccctcttgct ttataagttt
     1681 gcttacctga tggtaaattt caaactcggg gcctaattcg tttttaataa tggctgtttt
     1741 tacaccgcca ttcaaatctt caagcaactc tgaaaaaggt tctttttgta tgggcaattc
     1801 gtaagtgcgg tatgttttaa ttttagctaa atcggctgca atttttatgg catcatttag
     1861 tccgcccaat gtatcaacta accccaattg caaggcagtt ttaccactcc ataccctacc
     1921 ctgcccaatg ctatctacca tactttgtgg catgttacga ccattagcta cg
//
LOCUS       DAOMAA010000389         1972 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_58237_length_1972_cov_2.085550, whole genome shotgun sequence.
ACCESSION   DAOMAA010000389 DAOMAA010000000
VERSION     DAOMAA010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58237_length_1972_cov_2.085550"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1784)
                     /locus_tag="PLO59_05920"
     CDS             complement(<1..1784)
                     /locus_tag="PLO59_05920"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HQV00677.1"
                     /translation="MITRIEKVAILGSGIMGSRIACHFANAGIQVLLLDRCKDTLTDA
                     EVAKGLSANSMVIRNSIVNDALQQAVKSNPSPLYKKEYAKRITTGNFEDDMPAIANCQ
                     WVMEVVIENLEIKKTIFDAVEKYRKPQTIVSTNTSGIPVHLLTQNRSLDFKQHFCGTH
                     FFNPPRYLPLLEIIPGTETLPAVTQFLMHFGNLNLGKTTVLCKDTPAFIANRIGVFSI
                     IQLFNQVQQFKLSVEAIDALTGTILGRPKSATFRTTDVVGLDTLVHVANNLQTVLTSP
                     EKEQFQLPGFITQMVNNKWLGDKTKQGFYKKVKTENGKTEIHSLNLNTVTYHTQTKQK
                     FATVDAAKAIDNLPLRMQALINGSDVAAQFYKQSFATLFSYSAQQAMLIADNLYSIDD
                     ALRAGFGWELGPFEIWDAIGLQKGIDLIISHKQIVPQWVNDALKSGITSFYQVQKAQS
                     YCYHPGTNKYELIPGRQWQVDLNILRKTNKIWGNSGSTLTDLGNGIVNLEFHTKMNAV
                     GSEILQGINKAIDIAEQQGWRGVVIGNDAPNFSAGANLAMILMLAIEQEYDELDFAVR
                     AFQNTTMRIRYSSVPVVVAPHGLTLGGG"
BASE COUNT          581 a          389 c          365 g          637 t
ORIGIN      
        1 cagccaccgc caagtgttag tccatggggt gcaaccacta caggcacgct gctataacgt
       61 atgcgcatgg tggtgttttg aaacgcccga acggcaaaat ccagctcgtc gtattcttgc
      121 tcaattgcca acatcaaaat catggcaagg ttagcacctg ccgaaaaatt aggggcatcg
      181 ttaccaatta ctacaccacg ccagccttgt tgctcggcaa tatcaatagc tttgttgatg
      241 ccttgtaaaa tttcggagcc aacagcgttc attttggtat gaaactcaag gttaacaata
      301 ccgttaccca aatcggtaag tgtactgccg ctgttgcccc atattttatt ggtttttcgt
      361 aaaatgttta aatccacttg ccattggcgg cccggtatca gctcatactt attggtgccg
      421 ggatggtaac aataactttg tgctttttgt acctgataaa atgatgttat acccgatttt
      481 aatgcatcgt taacccattg tggtactatt tgtttatgcg atattattaa gtcaatgcct
      541 ttttgtaaac caatggcatc ccaaatttcg aacggcccca actcccatcc aaaaccggca
      601 cgtaaggcat catcaatact gtataaatta tcggcaataa gcatggcttg ttgagccgaa
      661 taagaaaaaa gtgttgcaaa cgattgttta taaaactgag ctgccacatc cgaaccgttt
      721 ataagtgcct gcatgcgtaa aggcaagtta tcaatagctt ttgcggcatc aaccgttgca
      781 aatttttgtt tggtttgagt atgataggta accgtattca ggttaagtga atggatttcg
      841 gttttgccat tttcagtttt aaccttttta taaaacccct gcttggtttt atcgcccaac
      901 catttattgt ttaccatttg tgtaataaaa ccgggcaatt gaaattgttc cttttcgggc
      961 gatgttaata ccgtttgcaa attattagca acatgcacca aggtatctaa accaaccaca
     1021 tcagttgtgc gaaatgttgc cgatttgggc cggcctaaaa tagtgcctgt taaagcatca
     1081 atggcttcga cacttaattt aaattgttgt acttgattaa ataattgaat gatagaaaaa
     1141 acaccaattc tgtttgcaat aaaagcaggt gtatctttac acaacaccgt ggttttacct
     1201 aaatttaaat taccaaaatg cattaaaaat tgggtaactg ccggtaaggt ttctgtaccg
     1261 ggtattattt ctaacaatgg caggtaacgt ggtgggttaa aaaaatgtgt tccacaaaaa
     1321 tgttgcttaa aatctaagct gcggttttgt gtgagcaaat gcaccggtat gcccgaagta
     1381 ttggtgctta caatggtttg tggcttgcgg tatttttcta ctgcatcgaa aatggttttc
     1441 ttaatctcta agttttcaat tacaacttcc ataacccatt ggcaatttgc aatggccggc
     1501 atatcgtctt caaaattacc tgtggttata cgttttgcat attccttttt atacagtggc
     1561 gatggatttg atttaacagc ttgttgcaaa gcatcgttta caatactatt gcgtataacc
     1621 atactgttag ccgataggcc tttggcaact tcggcatcgg ttaatgtatc tttacacctg
     1681 tctaacaata atacctgaat accggcattg gcaaaatgac aggcaatacg actacccata
     1741 atacccgagc ccagtatggc aactttttca atacgtgtta tcataattaa tttataaggt
     1801 ggctaaggta aattttgcaa ttaaagtttg atttaaatta taaatgaaaa ttgcgaaaac
     1861 ccatttttga agaattagta ttcaactttt tactttaacc ttaaatatta catttgcccg
     1921 tattcataaa ctaaaacatt aattacatga ccttaaacaa gtttcttatt ac
//
LOCUS       DAOMAA010000390         1970 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_58343_length_1970_cov_2.323760, whole genome shotgun sequence.
ACCESSION   DAOMAA010000390 DAOMAA010000000
VERSION     DAOMAA010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58343_length_1970_cov_2.323760"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1185)
                     /locus_tag="PLO59_05925"
     CDS             complement(<1..1185)
                     /locus_tag="PLO59_05925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQV00678.1"
                     /translation="MKILQLCIRIPYPPNDGGTIAMYNASVALQKANAQVTVLAFNTK
                     KHYITTQEIDKKYEAAFNLQTVYLDAAVKPIAAFINLFSNQSYNIQRFNSVAFTDALK
                     KNLQQQSFDVIQLESLFVAPYIATIRKYSKAKIVLRAHNMEYLIWERLALQEKSVLKK
                     WYLNLLSKRLKKFEQKVLTQIDALVTLTPDDEKGFKILGCNKPTLVAPIGIDVASYKA
                     IDLNTRPIQFFHLGSMDWLPNEEAVRWLLKEVMPIVKLKTTVDVSLAGKGMPKWIFDL
                     ADQHLQVEGLVSDAKSYMSKRHVMLVPILSGSGMRVKILEGLATGNVIISTTIGAEGI
                     KYTHGKNILIADTPQQFADGICMLLENPLLMEQISTQAKQLAQQYYDNETIGKSLISF
                     FTQ"
     gene            complement(1206..>1970)
                     /locus_tag="PLO59_05930"
     CDS             complement(1206..>1970)
                     /locus_tag="PLO59_05930"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HQV00679.1"
                     /translation="GKPNDTHQKIESKFDEELIRFDLSSFKLTRTNEELFKENKQMLN
                     VQQLTEAMDSLQLNLQSRKADYVRMYKPMLSYYRDSIEGQTVLAPVNALSDYEKAQQV
                     TILNNALAHARSLQAMYNNNADEYDMRYDNINRYEIEWHRKFTLSIACFVLFLIGAPL
                     GAIIRKGGLGVPIIVAIIFFLIFHVASITGEKMAKEGSATPISGMWAATAILLPIGLF
                     LIYKATTDSSIFDSEVYGRVIDFFRKRFTRKQNAVI"
BASE COUNT          596 a          357 c          334 g          683 t
ORIGIN      
        1 ctgtgtaaaa aaactaatta agcttttgcc aatagtttcg ttatcataat actgttgtgc
       61 taattgtttg gcttgggttg aaatttgttc catcaaaagc ggattttcta aaagcatgca
      121 aataccatct gcaaattgtt gcggtgtgtc agctatcaaa atatttttac catgcgtata
      181 ttttataccc tcggcaccaa tagttgtact tataataaca ttgcctgttg ccaaaccttc
      241 taatattttt acgcgcatac cactgcccga taaaattggc accaacatta catgccgttt
      301 actcatatag cttttagcat cgcttaccaa tccttctact tgtaaatgtt ggtcggctaa
      361 atcaaaaatc cattttggca tacctttgcc ggctaaactt acatcaactg tagttttaag
      421 cttaactata ggcataactt cttttaaaag ccatcttaca gcttcttcat taggcaacca
      481 atccattgaa cctaagtgaa aaaactgaat gggtctggta tttaagtcaa tggctttata
      541 cgatgcaaca tcaataccaa tgggcgcaac taaagtgggc ttattgcacc ctaaaatttt
      601 aaatcctttt tcatcatcag gtgtaagggt taccagtgca tcaatttgag ttaaaacttt
      661 ttgttcaaat ttttttaaac gtttgctcaa taaatttaag taccattttt ttagaacaga
      721 tttttcctgc aaagccaatc gttcccaaat taaatactcc atattatgag cacgtaaaac
      781 aatttttgct ttactgtatt ttctaatggt agcaatatat ggtgcaacaa ataagctttc
      841 taattgtatt acatcaaatg attgttgttg taaatttttt tttaaggcat cggtaaatgc
      901 aacagaatta aacctttgta tattataaga ttgattgctg aataaattaa taaaagccgc
      961 aatgggtttt acagccgcat ccaaatacac tgtttgaaga ttaaaagccg cttcatattt
     1021 tttatcaatc tcttgtgttg tgatgtaatg ctttttggtg ttaaaagcaa gaaccgtaac
     1081 ctgagcattt gctttttgta atgcaacgga agcattatac atggcaatag tgccgccatc
     1141 gtttggtgga tatggtatgc gtatgcagag ttgtaatatt ttcaaagtgt gatttacatt
     1201 acaaattaaa ttactgcatt ttgtttgcgt gtaaaccttt tgcgaaaaaa atctattaca
     1261 cgcccataaa cttcgctatc gaaaattgac gagtctgtag tggctttata aattaaaaac
     1321 aaaccaatag gcaataaaat ggctgtagca gcccacatgc ccgatatggg tgtggcactg
     1381 ccttctttgg ccattttttc gccagttatg gatgctacgt gaaatattaa aaagaaaata
     1441 attgcaacaa ttattggtac gcctaagcca ccttttcgga taatagcacc taagggagcg
     1501 ccaatcaaaa ataaaacgaa acaagcaatt gatagtgtga atttgcggtg ccattcaatt
     1561 tcgtatcggt ttatattatc gtatcgcata tcatattcat cagcattgtt attatacata
     1621 gcttgcaagc tacgggcatg tgccaatgca ttgtttaata tggttacttg ttgtgccttt
     1681 tcataatcgg atagggcatt aacaggcgcc aacacagttt gtccttcaat actatctcgg
     1741 taataagata acatgggttt atacatccga acgtaatctg ctttacggct ttgtaaattt
     1801 agctgaagac tgtccattgc ttcagttagt tgttgtacgt taagcatttg tttgttttcc
     1861 ttaaacagct cctcattagt acgtgttagc ttaaagctcg acaagtcgaa tctgattaat
     1921 tcttcatcaa atttgctttc aattttttga tgtgtatcgt tgggtttacc
//
LOCUS       DAOMAA010000391         1967 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_58506_length_1967_cov_2.838912, whole genome shotgun sequence.
ACCESSION   DAOMAA010000391 DAOMAA010000000
VERSION     DAOMAA010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58506_length_1967_cov_2.838912"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1344
                     /locus_tag="PLO59_05935"
     CDS             <1..1344
                     /locus_tag="PLO59_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00680.1"
                     /translation="NDALVILSLIKELQPSTYKILKQALNHTAMPVQFEAVKLSQRLH
                     AHELLADISQVAATTQSIALKQRCITAIYALSRNNITQLSWHKEQQWQIASLKGMLGN
                     ELLQHEAHKQLHVLFTQGKQSLIAALTIVQQSGNTHFSNEVMEALQSTDEEILALAIN
                     AAGVCHRAEIVHKLISLSEQTKYTASVMKSLSMQGGAAVKLIAEAISSKTLSLPTQKK
                     LIAVIAKANHTYSVPVLTTLANERTPLLHDIVFSLYVLGYKSAQTDKQFLQIIDNKFL
                     FASMLISQIQQVAMQQNKLLHNALTIELNDVRSQLLYMFTFFYNTENILRVKHAYVID
                     KPNVIANAMEVIDVTLNKSHAQKFIALFDNVSIETKLNEMQKYFHVQLQPQTVIKQIL
                     NAPEDTFNSWTKAVAVYTLSKDQTTHYNLRLKQIMTHADRLLQQTIVHKLQAIAH"
     gene            1354..1794
                     /locus_tag="PLO59_05940"
     CDS             1354..1794
                     /locus_tag="PLO59_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HQV00681.1"
                     /translation="MENGNLLLIEKVLLLKSISIFSDTPENILAQVAHLLHEIELDEN
                     VLIFNEGDIGNCMYIIYHGTVQINKGNQTLAVLNDKEFFGELSLLDTETRSASATTSK
                     PCFLLRIDQDPFFELLDTQPEIARGVIRILSKRLRNLNEKMLSK"
BASE COUNT          690 a          358 c          337 g          582 t
ORIGIN      
        1 aatgatgcat tggttatttt aagtttaatt aaggagttac agccatcaac gtataaaatt
       61 ttaaaacagg cattaaatca tactgcgatg cctgttcagt ttgaagcggt taaattaagc
      121 cagcgtttgc atgcccacga attgttggct gatataagcc aagttgcagc aacaacccaa
      181 agtattgccc ttaagcaacg atgtataaca gccatttatg ctttgtcgag gaacaacata
      241 acccaactca gctggcataa agaacagcag tggcaaattg caagtttaaa aggcatgctt
      301 ggtaatgaac ttttacaaca tgaggcacat aaacagttgc atgttttatt tactcaaggt
      361 aagcaatctt taatagctgc attaactatt gtacaacaat cgggcaacac acattttagt
      421 aatgaagtta tggaagcctt gcaaagcacc gatgaagaaa ttttagcttt agctataaat
      481 gcagcgggag tttgtcaccg ggccgaaatt gtacacaaac ttattagctt atccgaacaa
      541 actaagtaca cggcctccgt tatgaaaagc ttaagtatgc aaggtggtgc tgccgtaaaa
      601 cttattgccg aggctatttc aagcaaaaca ctatcgctac caacgcaaaa aaaattaatt
      661 gccgttattg caaaagctaa ccatacttat agtgtaccgg tacttacaac attggcaaat
      721 gaacgaacac cattattgca cgacatcgtt ttttcattat acgtattagg ttacaaatca
      781 gcccaaaccg ataagcagtt tttgcaaata attgataaca aatttttatt tgcatcaatg
      841 ttgatttctc agatacagca ggtagcaatg cagcaaaaca aactattaca taatgcacta
      901 accattgagc ttaacgatgt acgatcgcag ttgctttata tgtttacatt tttttacaac
      961 actgaaaata tattacgagt aaagcatgct tatgtaattg ataagcctaa cgtaatagcc
     1021 aatgcaatgg aggtaattga tgtaacatta aataaaagtc atgcacaaaa gtttattgca
     1081 ttgtttgata atgttagcat agaaactaaa cttaacgaaa tgcaaaaata ttttcacgtt
     1141 caattacaac cacaaacggt aatcaaacaa atactgaacg ccccggaaga tacattcaat
     1201 agctggacaa aagcagttgc agtttataca cttagtaaag atcaaacaac acactataat
     1261 ttacgcttga agcaaataat gactcatgcc gatagattgc tgcaacaaac aattgtacat
     1321 aagttgcagg ctatagccca ttaaattcgt atgatggaaa acggaaattt attattgatt
     1381 gaaaaagtac tgctactaaa atctattagt atattttcag atacacctga aaatatactg
     1441 gcccaggttg cgcatttact acacgaaatt gaattggatg aaaatgtatt gatatttaat
     1501 gaaggtgata tcggtaattg catgtacata atttatcatg gtacagttca aattaataaa
     1561 ggcaatcaaa cactggctgt acttaacgat aaagaatttt ttggcgagct atcgctgctc
     1621 gataccgaaa cacgctcagc ttctgcaact acatctaaac cctgtttttt attacgtatc
     1681 gaccaggatc ctttttttga actattagat acacaacccg aaattgcacg tggcgttata
     1741 cggattttga gtaaacgctt gcgtaacctg aatgagaaaa tgttaagcaa atagcttaat
     1801 aagcagcaac aatcttttca atttgttcca aatgtgtttg ctttaattgt tgccgttttg
     1861 cgcgcgaata gttcgtatgg taaaaatgtt gccttaaaaa acttagttga atattattcc
     1921 tcgcctcttc cgactgaatg ttaaaaaact gtttccactc ttcaaaa
//
LOCUS       DAOMAA010000392         1964 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_58692_length_1964_cov_2.181771, whole genome shotgun sequence.
ACCESSION   DAOMAA010000392 DAOMAA010000000
VERSION     DAOMAA010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58692_length_1964_cov_2.181771"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="PLO59_05945"
     CDS             <1..744
                     /locus_tag="PLO59_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00682.1"
                     /translation="PMKLAVGLLKNQKGNIDLKIPITGNLNDPNYNIGKVVWQVVKNI
                     LLKAATAPYKLVTNLFGGNEESYKSMAFDYKVADFGKEFYKVLDNLAELVKQKPEVNI
                     GLTQQSNMNVETEYLAELETKKRYYENIHGRMDSITPALADSLELINIKDSSFIAYVN
                     KQLNSNDLLTPIQQKCVQFIGQPALSIMVQKRMQYRNDLIKKYLGNIHKLPASRFNVS
                     NTTQQSFANTQSINVYVINYLANDSTQMQ"
     gene            796..1269
                     /locus_tag="PLO59_05950"
     CDS             796..1269
                     /locus_tag="PLO59_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00683.1"
                     /translation="MITWLVLSQANAQRFLALTKPGFNNRIRYYEGSRVGFKLINDKQ
                     VFKGNIMGFTNQGVLLNDSLLIPVQDVVAFYDYEHHRLPKFLSRLFISAGLVYGTVVL
                     VNGTLANSGDLQNSNNAIVVGSLLGVGVILLPFAQRKYKLNDKRWIKIIDVTITP"
     gene            complement(1282..>1964)
                     /locus_tag="PLO59_05955"
     CDS             complement(1282..>1964)
                     /locus_tag="PLO59_05955"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQV00684.1"
                     /translation="YNYNLPGNATEVFLKTPDGETINGIFIRQNKPKVIVYFHGNGTS
                     LLSWQRIADALLPHASLLIVDYRGYGKSSGNFSENGFYTDAETAIAFLLKEGFNMQQI
                     IVYGRSLGSGIAIEMAVRHTLGGLILESPYTSVVDVGKKQHPYLLPALLLKYRFYSLN
                     KAPAIKTPALIFHGEKDATIPVAQGRKIAAALPQCKLITIPDADHANVPAFEIYQQSL
                     AAFLNQLP"
BASE COUNT          645 a          376 c          381 g          562 t
ORIGIN      
        1 cccatgaaat tagccgtggg actacttaaa aatcagaaag gcaacattga tcttaaaatt
       61 ccgattacgg gtaacctaaa cgaccctaat tataatattg gtaaagtagt atggcaggta
      121 gtaaaaaaca ttttgcttaa agcagctact gcgccctata aattagtaac caacctgttt
      181 ggcggtaacg aagagtcgta taagtcaatg gcatttgatt ataaagttgc cgactttggc
      241 aaagagtttt ataaagtgct cgacaattta gccgaattgg taaaacaaaa acccgaagtt
      301 aatattgggc ttacacagca aagtaacatg aatgttgaaa ccgagtattt ggccgaatta
      361 gaaactaaaa aacgttacta tgaaaacata catggacgta tggatagtat tacacctgca
      421 ttagccgatt cgttggaatt aataaatatt aaagacagca gctttattgc ctatgtcaac
      481 aaacagctta acagtaacga tttacttaca cccattcagc aaaagtgcgt ccaatttatt
      541 ggccaacctg cacttagtat tatggttcaa aagcggatgc aataccgcaa cgacttaata
      601 aaaaagtatt taggaaacat tcataaacta cctgccagcc ggtttaatgt aagcaacaca
      661 acacaacaaa gctttgcaaa cacccaaagc attaatgtgt atgtaattaa ctatcttgca
      721 aatgatagca cacaaatgca gtaataaaac gtaacagtta ctatataggt gatgataaaa
      781 aggtgttttg tttttttgat aacatggctg gtgttaagcc aagccaacgc gcaacgtttt
      841 ttagcattaa ccaaacccgg atttaataac cggatacgat attatgaagg cagcagggtt
      901 ggctttaaac taataaacga caaacaggta tttaaaggca acattatggg ctttacaaac
      961 caaggtgttt tattaaacga ttcgttgtta ataccggtac aggatgtagt tgctttttat
     1021 gattatgaac atcatcgttt gcctaaattt ttaagcagac tatttatctc ggccggtttg
     1081 gtttatggta cggtagtgct ggttaatggc accttagcca acagtggcga tttgcaaaac
     1141 agcaacaatg ccattgtggt tggaagcttg ttgggtgtcg gtgtaatttt attaccattc
     1201 gcgcaacgta agtataaact taatgataag cgttggatta aaattattga tgtaaccata
     1261 accccctgaa aagcaactcc tttaaggcaa ctgatttaaa aatgcggcta atgattgttg
     1321 gtaaatttca aaagcaggca cattggcatg gtcggcatcg ggtatggtta tcaacttgca
     1381 ctggggtaat gccgcggcaa ttttacggcc ctgagctacc ggaatggtag catctttttc
     1441 gccatggaaa atgagtgccg gggtttttat tgcgggcgct ttattaagcg agtaaaaacg
     1501 atatttaagc aaaagcgctg gcaacaaata cggatgctgt tttttgccca catccactac
     1561 actggtgtat ggcgattcta atatcaaacc cccaagcgta tgccttaccg ccatttctat
     1621 ggcaatgcca ctccccaacg atcggccata aacaataatc tgttgcatat taaatccttc
     1681 tttcaataaa aatgcaatag ccgtttcggc atcggtataa aaaccgtttt cgctaaaatt
     1741 accactgctt ttaccataac ccctgtaatc aacaattaat aagctggcat gcggcaacaa
     1801 ggcatcggca atacgctgcc aacttaataa actggtgccg ttgccatgaa aataaacgat
     1861 aaccttcggt ttattctgcc taataaaaat gccatttatg gtttcgccat caggtgtttt
     1921 caaaaaaacc tctgttgcat ttccgggtaa attataattg tagt
//
LOCUS       DAOMAA010000393         1963 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_58723_length_1963_cov_3.331761, whole genome shotgun sequence.
ACCESSION   DAOMAA010000393 DAOMAA010000000
VERSION     DAOMAA010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58723_length_1963_cov_3.331761"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1067)
                     /locus_tag="PLO59_05960"
     CDS             complement(<1..1067)
                     /locus_tag="PLO59_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HQV00685.1"
                     /translation="MKSKHPAALPFLFFSEMWERFGYYLMIGIFFLYMTDTKTGGLGF
                     ERKEASDVFGTFIALVFLTPFVGGLLADRVLGYRLSITIGGILMGLGYCGLALEGMTS
                     FYVSLACIIVGNGFFKPNISTLLGNLYNTPEFKENKDAGYNIFYMGINIGAFICNFFA
                     AYLRNNFGWGYAFIAAGVGMFLGVIIFWLGARHYASGDVRKPTQPEDMSLPKIFSVVL
                     LPAFVAGYLGWIIPGNIFGSDSTDAFLFGALPITVFYVSVLIKARAEDKRPIAALLAI
                     FGVVIVFWAIFKQNGTALTIWAESYTDRQVPEVFYKPTKAIGMTQEIVYVKDSVQVLD
                     NQFRVAKDAAGNAVKELNYPA"
     gene            1385..1460
                     /locus_tag="PLO59_05965"
     tRNA            1385..1460
                     /locus_tag="PLO59_05965"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1418..1420,aa:Lys,seq:ttt)
     gene            1626..>1963
                     /locus_tag="PLO59_05970"
     CDS             1626..>1963
                     /locus_tag="PLO59_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00686.1"
                     /translation="MKSIIIKRSLKLFFMVCVLMCAYTGKAQDVSLDSLKNVLSQSVE
                     DTNKVKVLLELSRIMGNNATNEGLSYSILAKDLAIKLDYKKGLALAYKNIGKSYYILG
                     NYLEAFNAWD"
BASE COUNT          668 a          387 c          325 g          583 t
ORIGIN      
        1 taagccgggt agttaagttc tttaacagcg ttaccggctg catctttagc aaccctaaac
       61 tggttatcca aaacctgcac cgagtctttt acataaacaa tttcctgtgt catgccaata
      121 gctttggtcg gtttataaaa aacctcgggc acttgcctgt cggtgtaact ttcggcccag
      181 atagtaaggg cagtaccatt ttgcttaaat atagcccaaa acacaataac aacaccaaat
      241 atggctaata atgcagcaat gggcctttta tcttcggcac gtgctttaat taaaaccgat
      301 acataaaata cagtaatagg taatgcgcca aaaagaaaag catcggtact atcactgcca
      361 aatatatttc cgggtataat ccaccccaaa taccctgcaa caaatgcagg caatagcact
      421 actgaaaata ttttaggtaa gctcatatct tcaggttgtg taggtttacg cacatcgcca
      481 ctggcataat gccgggctcc taaccaaaaa ataattacac ctaaaaacat accaacaccc
      541 gctgcaataa aagcgtatcc ccaaccaaag ttattgcgta aataagctgc aaaaaagttg
      601 catataaatg caccaatgtt aatacccatg taaaatatat tatacccggc atctttattt
      661 tctttaaact caggggtgtt atacaggtta cctaaaagcg tacttatatt cggtttaaaa
      721 aaaccattgc ctacaataat gcaagccaat gaaacataaa atgacgtcat cccttcaagt
      781 gccaaaccgc aataaccaag ccccattaaa ataccaccaa tggttattga caagcgataa
      841 cccaaaaccc tgtcggctaa taagccacca acaaaaggtg ttagaaatac caaggctata
      901 aacgttccaa aaacatcgct ggcctcttta cgttcaaagc ctaagccgcc tgttttcgta
      961 tcggtcatgt aaagaaaaaa gatgcctatc attaaataat agccaaaacg ttcccacatt
     1021 tctgagaaaa ataaaaacgg caatgctgcc ggatgttttg atttcatcat acctgaattg
     1081 tatttaaatg ttgcaacaaa aatgaattaa ggcaggcaac ataatttaaa ttttaatatc
     1141 tgccgatttt ttgggaattg tgatgcaaag tttacggcat agctgcacag aaattaaaga
     1201 attgcaatgc tttttaatag gtaattgatt ttttttaaat acttcattga acgcaaatgc
     1261 ttaatttcat tgcatttgat aactatcaaa caacaaatac aaatacattt ttaaaaaaat
     1321 gttttaaatg ttttgtttgt aagtataaat tctatttttg cagcccgatt taaacatcgg
     1381 ttctgattcc gtagctcagc tggtagagca ttacactttt aatgtagtgg tcctgggttc
     1441 gaatcccagc gggatcacca gtatcttaaa agcccttatc tgttatatca ggtaagggct
     1501 tttaaatttt actacacaat gcgcagcatt ttattgttgc caaatactta atatttaaat
     1561 ccagcatctg attttttatt ttttctttgt tcattcacgc ccacaacacc acaaacattt
     1621 acaaattgaa atcaattatt atcaagcgta gcctaaaact gttttttatg gtttgtgtct
     1681 tgatgtgtgc atacaccggc aaggcacaag atgtttcgct ggatagttta aaaaatgtgt
     1741 tgtcgcaatc ggttgaggat accaataaag taaaagtact gttagagtta agccgcatta
     1801 tgggcaataa tgcaaccaat gaaggattaa gctatagtat tttggctaaa gacttggcca
     1861 taaagttgga ttacaaaaaa ggtctggcat tagcttacaa aaatatcggt aagtcttatt
     1921 acatcttagg caattattta gaagcgttta atgcctggga tca
//
LOCUS       DAOMAA010000394         1963 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_58755_length_1963_cov_2.317086, whole genome shotgun sequence.
ACCESSION   DAOMAA010000394 DAOMAA010000000
VERSION     DAOMAA010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58755_length_1963_cov_2.317086"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(258..1763)
                     /gene="dnaB"
                     /locus_tag="PLO59_05975"
     CDS             complement(258..1763)
                     /gene="dnaB"
                     /locus_tag="PLO59_05975"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014018408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HQV00687.1"
                     /translation="MSTGSENIKNIKRKIAKTGNTLPALELGKLPPQALDVEEAVLGA
                     LMLEKNAVNSIIDVLHGDVFYSEAHKLIFQAIHDLFHGAKPVDILTVTQHLKSEGTLE
                     FVGGPYYIAQLTNRVASAANIEFHARIIIQKFIQRELIRISTDISREAYEDTSDVIEL
                     LNRAEKNLFAIAEGNLRKKYDGLQGLISKAIKEYEHAATMPEGLSGIPSGFTSLDRVT
                     GGWQKSDLIIAAARPGMGKTAFVLSLARNAALRFNKPVAFFSCEMSSLQLVNRLIASE
                     TEISSEKLRKGNLSDAEWQQLNSKISPLEKAPIYIDDTPALTVFDLRAKCRRLVAEKK
                     IELIIIDYLQLMQTGDTRAGNREQEISIISRTLKAIAKELDIPIICLSQLSREVEKRG
                     GPKKPMLSDLRESGAIEQDADMVLFIYRPEYYDQTQDADGVNTQGLAEIIVAKNRNGS
                     LQSVNLRFIGHLTKFTDFEFDYSDQSGLITQRHEPERMTRGSKINDDGPDF"
BASE COUNT          499 a          394 c          389 g          681 t
ORIGIN      
        1 aatcaatacg ctcgcgggca agcattttat ttttttcttt ttgtttttgg gctgcttttt
       61 cgccaccacc tttataagtt ttatctaact taaactgtaa ttcgcttaac agcgttttca
      121 tgctgtcttc gtttatgttt tgttctaaat tcatacaaat atgggtatgt gcagtatgcc
      181 aaaaataaaa actgtttcga attttattgc tttaactttc gctgtaaaat ggttttatga
      241 tgtgccttta tcaattatta aaagtcgggg ccatcgtcat taatttttga gccgcgtgtc
      301 atacgttcgg gctcgtgtcg ttgtgttatc aggcctgatt ggtcgctata atcaaactcg
      361 aaatcggtaa acttggtaag gtgtccaata aaacgaaggt ttacactttg tagtgagcca
      421 tttcggtttt tagctacaat aatttcggca aggccttgtg tgtttacacc atcggcatct
      481 tgtgtttgat cataatattc gggtcggtag ataaacaata ccatgtcggc atcttgttcg
      541 attgcgcccg attcgcgtaa gtcgctaagc atgggctttt tggggcctcc gcgtttttca
      601 acttcacgac tcaactgact taaacaaata atgggtatat ctaattcttt ggcaattgct
      661 ttaagtgtac gcgatataat agaaatttcc tgctcgcggt taccggcacg tgtatcgcct
      721 gtttgcatca actgtaaata gtcaattatt ataagttcta tctttttttc ggcaaccagt
      781 cttcggcatt tggcacgtaa atcaaaaacg gttaatgccg gtgtatcgtc aatataaata
      841 ggcgcttttt cgagcggact aattttgctg tttagttgtt gccattcggc atcgcttaaa
      901 tttcctttac gtaatttttc gctgcttatt tcggtttcgc tggctataag cctgtttact
      961 aattgcagtg acgacatttc gcaactaaaa aatgcaaccg gtttattaaa cctaagcgca
     1021 gcgttgcgtg ccaacgataa tacgaatgcc gttttaccca taccagggcg tgctgctgca
     1081 ataatcaggt cggatttttg ccagccaccg gttaccctgt ctaatgatgt aaagccgctg
     1141 ggtatgccac ttaaaccttc gggcatggtg gcagcatgtt cgtattcctt tattgcttta
     1201 cttattaagc cttgtaggcc atcgtacttt ttacgcaggt tgccttcggc aattgcaaac
     1261 aggttttttt cggcacggtt taaaagttca attacatcgc tggtgtcttc atacgcttcg
     1321 cgtgatatat cggtgctgat gcggattaac tcgcgctgaa taaacttttg aataattatt
     1381 cgtgcatgaa actcgatgtt ggcagcactg gcaactctat tggtaagttg ggctatataa
     1441 taagggccac ccacaaactc aagcgtgcct tcgcttttta agtgttgtgt tacggtaagt
     1501 atatcaaccg gcttggcgcc atgaaacaaa tcgtgtatgg cctgaaatat gagtttatgt
     1561 gcttcgctgt aaaacacatc gccatgcaat acatcaataa ttgagtttac tgcatttttt
     1621 tcgagcatta atgcgcctaa cacagcttct tcaacatcaa gcgcctgcgg gggtaatttg
     1681 cctaactcca aagccggcaa tgtattaccc gttttggcaa ttttgcgttt tatgtttttt
     1741 atgttttctg atccggttga catgaatcaa tttctaaata agattgcaac tgatttttaa
     1801 aaagggacac gaaagtatta atgtactcgg ttactaccat ttatttatta gtttattttt
     1861 ttttccacaa tgttgaatcg gcaacaaaaa aaacagaatg cgccattggt aacatttacg
     1921 tacattatta acattgcatt tcataaaacg tcccccttgt att
//
LOCUS       DAOMAA010000395         1960 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_58919_length_1960_cov_2.535958, whole genome shotgun sequence.
ACCESSION   DAOMAA010000395 DAOMAA010000000
VERSION     DAOMAA010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58919_length_1960_cov_2.535958"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="PLO59_05980"
     CDS             <1..418
                     /locus_tag="PLO59_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015332820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HQV00688.1"
                     /translation="HYYFLLLNITYVIYFIWLLYEILIYLVKPSYINTDIISAAACGY
                     LLLIETCTFTMQYFVYKNEQSFNGINLANPASTYMDLVYFSSITLTSIGFGDITPNTH
                     HTKLLTSLFGITGQFYIVILVGILVSKFTSNATQSK"
     gene            424..1428
                     /locus_tag="PLO59_05985"
     CDS             424..1428
                     /locus_tag="PLO59_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016199583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family ion channel"
                     /protein_id="HQV00689.1"
                     /translation="MHTGKRYTPFEFFYWTRRITFKLFLIAIVPTLLYSLGWKFITIP
                     WQPVAILGTAVAFVVGFKNNASYGRIWEARQIYGSIINDSRSFAYTVRDIVGSKNNMD
                     VKIIFYRHFAWLTCLRHQLRESRTWENMDSMHNKEYLRKYRIPEKAENLNEILKQLLS
                     EQDYNYVITKKNKATQLVAMQSAAIANMYKADKINDVAWKQLQECLIKFTDGQGRAER
                     IKNFPYPRNFASITTYLLFIFVISVPFTLLPEFNKLGLDTFLEGYSLWFNVPFCTMIM
                     WVFVTLDVIGESSVNPFEGSSLDVPITQISRTIEIDMRDMLDETELPPAIQPVNNIVL
                     "
     gene            1439..>1960
                     /locus_tag="PLO59_05990"
     CDS             1439..>1960
                     /locus_tag="PLO59_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015332822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00690.1"
                     /translation="MKKRDKLSIIHKWYPNAITTIDSVNRIIDYVEYELDLEPKQVML
                     ADSICSDDVNSIQYPARTQEFLGPFKMGGLDGFPFTGLTGMGAFASHVPDDGAVFIYY
                     GPHIGIAKDGTIGEIHRFGQSKNSGCCGAAKGALNKLINNIIIPGNVTELDYQMNTIV
                     QILLNESNRILHAK"
BASE COUNT          647 a          355 c          356 g          602 t
ORIGIN      
        1 tcactactac tttttattac tcaatataac gtatgtgata tactttattt ggctacttta
       61 tgaaatactc atttatttgg taaagcccag ttacattaat acagatatta tttcggctgc
      121 agcctgcgga taccttttac ttattgaaac gtgcaccttt actatgcagt atttcgttta
      181 caaaaatgag caatcgttta atggaattaa tttagctaac cctgccagca cttatatgga
      241 cttggtatat tttagctcta taacgcttac cagtattggc tttggcgaca ttacacccaa
      301 tacacatcat actaaattac tcacttcgtt atttggcata accggtcaat tttatatcgt
      361 tatactggta ggaattttag taagcaaatt cacttcgaac gctacacaat caaaataacg
      421 aacatgcata caggtaaacg atacactcct ttcgaatttt tttactggac aagaagaatt
      481 actttcaaac tttttcttat tgcaattgtt ccaacattat tgtacagtct gggatggaaa
      541 tttataacta ttccatggca acccgttgca atattgggca cggctgttgc ctttgtagta
      601 ggctttaaaa acaatgcaag ttatggccgt atttgggagg cacgtcaaat ttacggcagc
      661 ataataaacg atagcagaag ctttgcatat acagtaagag atattgttgg cagcaaaaac
      721 aatatggatg taaaaattat tttttaccgg cattttgcct ggcttacttg tctcaggcat
      781 caattacgtg aaagccgcac ctgggaaaac atggattcga tgcataacaa ggaatattta
      841 aggaaatata gaattcctga aaaagcagaa aacctgaatg aaatattaaa acaacttcta
      901 tctgaacagg attataatta tgttatcaca aaaaaaaata aagctacgca acttgtagcc
      961 atgcaatcag cagcgattgc aaacatgtat aaagcagaca aaataaacga tgtagcatgg
     1021 aagcaattgc aggaatgcct aataaaattt acggatggac aaggcagggc tgagcgaata
     1081 aaaaattttc cttatccgcg taactttgca tcaataacca cctacttact ttttattttt
     1141 gttatcagtg tgccatttac tcttttacct gagtttaata agcttggcct tgatacattt
     1201 ttagaaggct actccttatg gtttaatgta cctttttgta cgatgataat gtgggtattt
     1261 gtaacacttg atgtaattgg cgaaagttcg gttaacccgt tcgaaggcag ttcgttagat
     1321 gttcctatta cacaaataag ccgtaccatc gaaattgata tgcgcgatat gttagatgaa
     1381 accgaattgc cacctgccat acaacccgtt aataatatcg ttctataaat ttaatattat
     1441 gaaaaaaaga gataaactaa gcatcattca caaatggtat ccaaatgcga ttaccactat
     1501 tgacagtgtt aaccgtataa ttgattatgt tgaatatgaa ttggatttag aacccaagca
     1561 agtaatgctg gctgatagca tttgcagtga tgatgtaaac agcattcaat acccggcacg
     1621 tacgcaggaa tttttaggcc catttaaaat gggcggctta gatggatttc cgtttacagg
     1681 ccttaccggc atgggcgctt tcgcaagcca tgtacctgat gatggtgctg tatttattta
     1741 ctatggcccg catattggca ttgctaaaga tggcactata ggcgaaattc atcgcttcgg
     1801 acagagtaaa aacagcggtt gttgtggtgc cgcaaaaggt gcattaaaca aattaataaa
     1861 caacatcata atacccggaa atgttacgga actagattac caaatgaata ccattgtgca
     1921 aatacttttg aatgaaagca acagaatact gcatgcaaaa
//
LOCUS       DAOMAA010000396         1959 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_58999_length_1959_cov_2.450630, whole genome shotgun sequence.
ACCESSION   DAOMAA010000396 DAOMAA010000000
VERSION     DAOMAA010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58999_length_1959_cov_2.450630"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..695)
                     /locus_tag="PLO59_05995"
     CDS             complement(114..695)
                     /locus_tag="PLO59_05995"
                     /inference="COORDINATES: protein motif:HMM:NF036997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00691.1"
                     /translation="MYVDVVLPLALNKTFTYSVPAHQQNDVAIGKRVLVHFKGNKIYA
                     AIILNYHKNAPVTYEAHDIIDIIDLMPVINNKQLLLWHWMAGYYLCTLGEVMAAALPA
                     ALKLSSESVVQLHPDFVETQHILTQREFVLIEALHQKSTLTLYEVSKLLGGKVAHRML
                     QQMVDKQMVIIFIFQKIIYFCTIKDHRHTKIQP"
     gene            complement(699..1874)
                     /locus_tag="PLO59_06000"
     CDS             complement(699..1874)
                     /locus_tag="PLO59_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010229221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HQV00692.1"
                     /translation="MNQNTFEMIAKTFSGMEDVLMYELENLGATNCTKLTRAVSFTGD
                     KALMYKANLWCRTALRILVPFKSFEVSNEQSLYDEIQKIDWSVWLTTESTLAIETTLN
                     QTVFNHSQFVSQKIKDAIVDQFRARFDKRPSVDLRQPDLRIQAHIYDNKCTLSFDSSG
                     ESLHKRGYRDLTNEAPINEALAAGIIKLTGWKGDSPFVDFMCGSGTFLIEAAMIARNM
                     APNKFRKQFGFQRMPDYDEELWQNIYDDALIGEKESIDFEISGGDKNGMVLQKAQQNI
                     NNAGLQHDIKLTKTRFENFMPPEDAGIVITNPPYGLRLQENDLMQMYQEIGNTLKQQY
                     AGWQAWVFTGNLNLFKFIGLRPTRKIHLYNGAVECRLLRFDMYSGTKRIHKFRNDIA"
BASE COUNT          561 a          369 c          361 g          668 t
ORIGIN      
        1 tgattgcaac gcttggctgg gtggcggttt ctgtagggtg ttctatttat atggataact
       61 ttacgaattt ctcatatatt tatggaagta tggcaggaat tatgattctt cttttatggc
      121 tgtatttttg tatgtcgatg gtcttttatg gtgcagaagt aaattatttt ctggaaaata
      181 aagataatta ccatttgctt atcaaccatc tgctgtaaca tacggtgtgc aactttgccg
      241 cctaaaagtt tagaaacttc atatagtgta agtgtacttt tttgatgtaa ggcttcaatc
      301 aatacaaatt cccgctgggt aagtatatgc tgtgtttcaa caaaatcggg gtgtagttgc
      361 accacgcttt cgctcgataa ttttagtgct gccggtaaag cagcggccat tacttcgcct
      421 aaagtacaca ggtagtaacc tgccatccaa tgccaaagca ataactgctt attgtttatt
      481 acaggcatta aatcaataat gtcaataata tcgtgtgctt cataagtaac gggtgcattt
      541 ttatgataat ttaaaataat ggctgcataa attttattgc ccttaaagtg caccaatacg
      601 cgtttaccta tagcaacatc gttttgctga tgcgcaggta cgctgtaggt aaatgtttta
      661 tttaatgcca ggggcaatac cacatcaaca tacataggct aagcaatgtc gtttctgaat
      721 ttatgaatac gttttgtacc actatacata tcaaaacgta acaacctgca ttcaacagct
      781 ccgttgtaca aatgtatttt tcgggtaggg cgcaggccaa taaatttaaa caggtttaag
      841 ttgccggtaa acacccaggc ttgccagcct gcatattgtt gttttaatgt gttgccaatt
      901 tcctgataca tctgcatcaa atcgttttcc tgtaagcgca aaccataggg tgggttggta
      961 ataacaatgc cggcatcttc aggtggcata aagttttcga aacgtgtttt tgttaattta
     1021 atatcgtgct gcaaccctgc attgtttata ttttgttgcg ctttttgtaa aaccattcca
     1081 tttttatcgc cccccgaaat ttcaaaatca atactttctt tttcaccaat caaagcatca
     1141 tcataaatat tttgccatag ttcttcatca taatcgggca tacgttgaaa accaaattgt
     1201 ttacgaaatt tattaggtgc catattgcgt gcaatcattg ctgcctctat taaaaaagtg
     1261 ccactgccac acataaaatc tacaaagggg ctatctcctt tccaaccggt taacttaata
     1321 atgccggcag ctaaagcttc gtttatgggg gcttcgttgg ttaaatcgcg atagccgcgt
     1381 ttgtgtaaac tttcgcccga actatcgaat gacaaagtgc atttattatc gtatatgtgc
     1441 gcttgtatgc gcaaatcggg ttgtcgtaaa tcaacactgg ggcgtttatc aaatcgggca
     1501 cgaaactgat ctacaatggc gtctttaatt ttttgtgata caaattgcga gtggttaaaa
     1561 accgtttggt ttaaggtggt ttcaatggct aatgtacttt cggttgtaag ccaaaccgac
     1621 caatcaattt tttgtatttc atcgtataat gattgctcat tacttacttc aaaactttta
     1681 aatggtacta aaatacgtaa tgcggtacgg caccataaat tggctttata cattaaagct
     1741 ttatcgcctg taaaagaaac tgcgcgtgtt agtttggtgc agttggttgc gcctaaattt
     1801 tctaactcat acattaaaac atcttccata cctgaaaatg tttttgctat catttcgaat
     1861 gtgttttgat tcattactgt tttgtattaa tcttaattac ttaaccatcc tttacgggta
     1921 aaataccaaa gttcaactgc cactattaaa aacatacca
//
LOCUS       DAOMAA010000397         1959 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_59004_length_1959_cov_2.227416, whole genome shotgun sequence.
ACCESSION   DAOMAA010000397 DAOMAA010000000
VERSION     DAOMAA010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59004_length_1959_cov_2.227416"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..1843
                     /gene="ettA"
                     /locus_tag="PLO59_06005"
     CDS             164..1843
                     /gene="ettA"
                     /locus_tag="PLO59_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HQV00693.1"
                     /translation="MSDDKKIIFSMVGLNKVYPNGKQVLKNIYLSFYYGAKIGILGLN
                     GAGKSTLLRIIAGTEKSYQGEVVFAQGYTVGMLEQEPLLDDTKTAKEIVLEGVERITA
                     ILKEYEAVNEKFSDPDLDGDAMDKLIARQGQLMDEIEAAGAWELDTKIEYAMDALRCP
                     EPDTPVKNLSGGERRRLALCRLLLQEPDILLLDEPTNHLDAESVDWLEQHLQRYKGTV
                     IAVTHDRYFLDNVAGWILELDRGEGIPWKGNYSSWLEQKGKRLAQEEKTESKRQKTLA
                     RELEWVRMSPKARHAKSKARLQAYENMLSEDVKQKEEKLELFIPNGPRLGSEVIEFTN
                     VSKAYGDKLLYENLSFKLPPAGIVGIIGPNGAGKTTLFRMIMGQETPNAGTINIGSTV
                     KIAYVDQQHAGINAEHTIWEAITGGNEVLTFDGKTINSRAYVGRFNFSGADQGKKVNV
                     LSGGERNRLHLALTLRSEANVLLLDEPSNDIDVNTLRALEEALEDFAGCAVIISHDRW
                     FLDRICTHILAFEGDSQVYYFEGGYSDYEENKKKRLGDTGPHRIKYKSLVK"
BASE COUNT          642 a          379 c          429 g          509 t
ORIGIN      
        1 atcacgccaa ttacccagct atgggttatc ctataacaaa gtaggcaact gtaaggaaaa
       61 aaattacttt cgcagcccgc ttaaaagccc gctttaattt ggtggttgcg catcatcaaa
      121 atgggtattt actgaaccgt aattatttaa aaacacaaac atcatgagtg acgacaagaa
      181 gattattttt tcgatggtag gccttaataa agtttacccc aacggtaaac aggtattaaa
      241 aaatatttat ttatcgtttt attatggtgc aaaaattggc atcttagggt taaacggtgc
      301 cggtaaatca acactgttgc gcataattgc aggtaccgaa aaatcgtatc agggcgaagt
      361 agtttttgca cagggatata ccgtagggat gttggaacaa gaacctttat tggatgatac
      421 caaaacggca aaggaaattg tactcgaagg tgttgaacgc attacagcca tacttaaaga
      481 gtatgaggcc gttaacgaaa aatttagcga ccccgattta gatggcgatg ctatggataa
      541 acttattgca cggcaaggtc agcttatgga cgaaatagaa gcagcaggcg cttgggaatt
      601 agatacaaaa atagaatatg caatggatgc attgcgttgt cccgaacccg atactccggt
      661 aaaaaacctg tcgggtggcg agcgcaggcg tttggcactg tgccgcttat tattgcaaga
      721 accggatatc ttattgttag atgaacctac caaccattta gatgccgaaa gcgttgattg
      781 gttagaacaa catttgcaac gctataaagg aaccgtaatt gcagtaacac atgatcgcta
      841 cttcttagac aatgttgccg gatggatttt agaattagac cgtggcgaag gcattccatg
      901 gaaaggaaat tactcaagct ggttagagca aaaaggcaaa cgcttagccc aagaagaaaa
      961 aacagaaagc aaacgccaaa aaactttagc acgcgaatta gaatgggtgc gcatgagccc
     1021 caaagcccga catgcaaaat caaaagcacg tttgcaggct tatgaaaaca tgttaagcga
     1081 agatgtaaaa caaaaagaag aaaaattgga gttatttatt cctaatggcc cacgtttagg
     1141 tagtgaggta atcgaattta ccaacgtatc gaaagcctat ggcgataaat tactttacga
     1201 aaatctatca tttaaattac caccagccgg tatagttggt attattggcc ccaacggagc
     1261 gggtaaaacc actttgttcc gcatgattat gggccaggaa acacccaatg caggcactat
     1321 taatattgga agtacggtta aaattgctta tgttgatcag caacatgcag gcataaatgc
     1381 cgagcatacc atttgggaag ctattacagg cggaaatgaa gtattaactt tcgatggcaa
     1441 aacgattaac tcacgcgctt atgtaggccg ttttaatttt tcgggtgccg accagggtaa
     1501 aaaagtgaat gtgctttcag gtggcgaacg taaccgctta catttggcgc ttaccttaag
     1561 aagcgaagcc aacgtactgc tacttgacga acccagtaac gatattgatg taaacacttt
     1621 gcgtgcattg gaagaagctt tagaagattt tgctggttgt gcagtaatta tcagccacga
     1681 ccgttggttt ttagaccgta tttgtacaca tatacttgct ttcgaaggcg actcacaagt
     1741 atattatttc gaaggcggct acagcgacta tgaagagaat aagaaaaaac gattgggcga
     1801 taccgggccg catcgtatta aatataaaag cttggttaag taatgaattc aaacagcaac
     1861 ggctgcccaa gggcagccgt tgctgtttca cacctttcaa ctaacccctt tcaactctca
     1921 actcctttta tatctttacc actttcaaaa ccaagtttc
//
LOCUS       DAOMAA010000398         1957 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_59100_length_1957_cov_2.601472, whole genome shotgun sequence.
ACCESSION   DAOMAA010000398 DAOMAA010000000
VERSION     DAOMAA010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59100_length_1957_cov_2.601472"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /gene="gmd"
                     /locus_tag="PLO59_06010"
     CDS             complement(<1..935)
                     /gene="gmd"
                     /locus_tag="PLO59_06010"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015813402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HQV00694.1"
                     /translation="MKVALITGITGQDGAYLAEFLLAKGYMVHGIKRRSSLFNTERID
                     HIYSDSHEKNVKFKLHYGDLTDSANIIRIIQEVQPDEIYNLGAMSHVKVSFNTPEYTA
                     NADGIGALRILEAVRMLGMTQHCKIYQASTSELYGLVQEIPQKETTPFYPRSPYAVAK
                     LYAYWIMVNYREAYNMFAVNGILFNHESPLRGETFVTRKITRAVARIALGLQELVYMG
                     NIDAKRDWGHAADYVEAMWLMLQQDKPKDYVIATGVTTTVREFIKLSFEQVGIEVEFS
                     GTAENEIATVIQCQMQAYQIPKGTVVMRIDAQYYRP"
     gene            complement(1083..>1957)
                     /locus_tag="PLO59_06015"
     CDS             complement(1083..>1957)
                     /locus_tag="PLO59_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008508751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HQV00695.1"
                     /translation="SRKPIFGYKAMIGSMGLIAFLSCIVWGHHMFITGMNPFLGSVFV
                     VTTLMVAIPSAVKVFNYIATLWRGNIVLTPAMLFAIGLVSFFISGGLTGIILGDSALD
                     INIHDTYFVVAHFHIVMGASAIFGMLAGVYHWFPRMFGRLMNKNLGYIHFWLTLISVY
                     GVFFPMHFIGLAGVPRRYYANTAYAGFDNLVGINELITVFALLSGVAQLVFAFNFFYS
                     IFKGKKSPQNPWKSNTLEWTAPLSDVHGNWPGEIPTVHRWPYDYGKPGHEDDFIPQTV
                     PFSATPGSNRPDDH"
BASE COUNT          623 a          409 c          339 g          586 t
ORIGIN      
        1 ggtcggtaat attgagcatc tatacgcatt actactgtgc ctttaggaat ttgatatgct
       61 tgcatctgac attggattac ggttgcaatt tcgttttctg ccgtgcccga aaactcaacc
      121 tctattccaa cttgttcaaa tgataattta ataaattctc taaccgtggt ggtaacaccg
      181 gtggcaatta cataatcctt aggtttatct tgttgaagca ttaaccacat ggcttctaca
      241 taatcagcgg catggcccca gtcacgtttt gcatctatat tacccatata aaccaattcc
      301 tgtaaaccca atgcaattcg agcaactgcc cttgtaattt ttcgggtaac aaaggtttcg
      361 ccacgaagtg gcgattcatg attaaataaa ataccattaa cagcaaacat gttataagcc
      421 tcccggtaat ttaccattat ccaatatgca taaagctttg ccacagcata aggtgaacgt
      481 gggtaaaaag gcgtagtttc tttttgtggt atttcctgta ccaatccata cagttcactg
      541 gtgcttgctt gataaatttt gcaatgttgt gtcataccca acatgcgtac tgcttctaat
      601 atgcgaagtg cccctatacc atcggcatta gccgtatatt ccggtgtgtt gaagctaacc
      661 tttacatggc tcattgcgcc caggttataa atttcatccg gttgtacttc ttgtatgatt
      721 ctgataatgt ttgctgagtc ggttaaatcg ccataatgca gtttaaattt cacatttttc
      781 tcgtggctgt cgctgtatat atgatcaata cgttcggtat taaataatga acttctgcgt
      841 tttataccat gtaccatata tccttttgct aacaaaaact cagctaaata ggcaccatct
      901 tgtccggtta ttccggtaat aagagcaact ttcatactta tatcacttaa aaatgtttca
      961 aatattggtt acaaataaaa ttaaatctta aagtcatttt tgtaatacta aaaaagcata
     1021 aaaccaaaaa aatccccctg ttagttaaaa cagggggttt attagttttt caaacaaccg
     1081 ttttaatgat catcaggcct attgctacct ggtgttgccg aaaatggcac cgtttgagga
     1141 atgaagtcat cctcatggcc tggtttgcca taatcatacg gccaacgatg taccgttggt
     1201 atttcaccag gccagttgcc gtgtacgtca ctaagaggtg cagtccattc cagcgtattt
     1261 gatttccaag ggttttgtgg agattttttg cctttaaaaa tgctgtagaa aaagttaaac
     1321 gcaaaaacca actgtgccac accacttaac aatgcaaaaa cagtaataag ttcgttaatg
     1381 ccaactaagt tgtcgaaacc tgcataagct gtatttgcat aatatctgcg tggcacaccg
     1441 gctaaaccga taaaatgcat tggaaagaat actccatata cagaaatcaa ggtcaaccaa
     1501 aaatgtatgt accctaaatt cttattcatt aagcgaccaa acatacgtgg aaaccaatgg
     1561 taaactccag ctaacattcc aaaaattgcc gaagcaccca taacaatatg gaagtgagct
     1621 accacaaaat aggtgtcgtg tatattaata tctaatgccg agtctcctaa aataataccg
     1681 gttaatccac cgctaataaa aaacgaaacc aagccaatgg caaataacat agcaggtgtt
     1741 agcacaatgt ttccacgcca taatgtagca atgtaattaa atacctttac tgcagatggg
     1801 atggcaacca ttaaggttgt tacaacaaac acggatccta aaaatggatt catccctgta
     1861 ataaacatgt ggtgccccca aacaatacat gataaaaagg ctattaagcc catagatcca
     1921 atcattgctt tatagccaaa aattggcttt cgcgaat
//
LOCUS       DAOMAA010000399         1956 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_59148_length_1956_cov_2.652814, whole genome shotgun sequence.
ACCESSION   DAOMAA010000399 DAOMAA010000000
VERSION     DAOMAA010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59148_length_1956_cov_2.652814"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1940)
                     /gene="gcvP"
                     /locus_tag="PLO59_06020"
     CDS             complement(<1..1940)
                     /gene="gcvP"
                     /locus_tag="PLO59_06020"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008990817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HQV00696.1"
                     /translation="MLTTIGVASIEELISQTVPKSIRIKGDLNLPEAMSEAAYLKFIK
                     GVSMKNKVYRTYIGLGYYNTIVPGVIQRNIFENPGWYTAYTPYQAEISQGRMEALLNY
                     QTMVCDLTGMELANASLLDEATAAAEALHMFFASRTKEQEKANANKFFVSDACFPQTI
                     DVLKTRANPIDVELVIGNHNNIVFDETYFGALIQYPTITGDVYDYTNFVANAKKQQLM
                     ICVAADIMSLALLTPPGQWGADCVVGSTQRFGVPLGYGGPHAAYFATHQKFKRDIPGR
                     IIGVSVDTTGKRALRMALQTREQHIKRDKATSNICTAQVLLSVMASMYAVYHGQAGIR
                     HIATTIHHNAGYLKAALMQLGYNVTTGAFFDTITISTPTENINKFALEQKINLRYFES
                     GVSISIDETTDASDLADLISIFAKAANKTFNPVQIQAETHFGAQGKHLLNRTTPYLTF
                     DVFSKNRSEHDMLRYIKRLENKDLAMNHSMISLGSCTMKLNATTEMAPLSWSHFSNIH
                     PFVPLDQAAGYAQMITELEQYLAEITGFAAVSLQPNSGAQGEYAGLLVIRAYHQLQGQ
                     GHRNIALIPQSAHGTNPASAVMCGYQIVVVKCDEKGNIDVADMREKAAKHSNNLACLM
                     VTYPSTHGVYEEGISEITSIIH"
BASE COUNT          539 a          401 c          398 g          618 t
ORIGIN      
        1 tgatggatta ttgaagttat ttctgaaata ccttcttcat acacaccatg ggttgatggg
       61 taagtaacca taaggcaagc taaattattg ctatgctttg ctgccttttc gcgcatatcg
      121 gcaacatcaa tattgccttt ttcatcacat ttaactacaa caatttgata gccacacata
      181 acagcgcttg caggattggt gccatgtgcc gattgaggta taagtgctat gttacgatgc
      241 ccttggccct gtagttggtg atatgcccta attaccaata agccggcata ctcgccttgt
      301 gcgcccgaat tgggttgtaa cgatactgca gcaaaaccgg tgatttcagc taaatactgc
      361 tccaactccg ttatcatttg tgcatatccg gcagcctggt ctaatggtac aaagggatgg
      421 atattgctaa agtgcgacca gcttaatggt gccatttctg tggtagcatt cagtttcatg
      481 gtgcatgaac ctaaagatat catgctatga ttcatagcta aatctttatt ttctaatctt
      541 ttgatgtaac gtagcatatc atgttcgcta cgatttttac taaacacatc aaatgtgagg
      601 tagggtgtgg ttcggtttaa aagatgtttg ccttgtgccc caaaatgtgt ttcagcctga
      661 atttgtacgg ggttgaaagt tttattggct gctttggcaa aaatggaaat caaatcggct
      721 aaatcgcttg catccgttgt ttcgtcaata cttatactta cgccactttc aaaatatcgg
      781 aggtttattt tttgctcaag cgcaaacttg tttatatttt cagtgggcgt tgatatagtg
      841 atggtatcaa aaaatgcacc ggtggtaaca ttatagccta attgcattaa ggctgctttt
      901 aaatatccgg cattgtgatg tatggttgta gcaatatgcc tgatgccagc ctgaccatga
      961 tatactgcat acatacttgc cattaccgag agtaaaacct gtgctgtaca aatatttgaa
     1021 gtagccttgt cgcgctttat gtgttgttcg cgcgtttgca atgccatacg caacgcacgt
     1081 tttccggtag tatcaacact aaccccaata atgcggccgg gtatatcgcg tttaaatttt
     1141 tgatgagttg caaaataggc agcatgtggc cctccatatc ctaaaggcac tccaaaacgt
     1201 tgtgttgaac ctaccacgca atcggcaccc cattgacccg gtggtgtaag tagggctaag
     1261 ctcataatat cggcagctac acaaatcatc aattgctgtt tttttgcatt tgctacaaag
     1321 ttggtatagt cgtacacatc gcctgtaata gttgggtatt gtataagggc accaaaatat
     1381 gtttcgtcaa aaacaatgtt gttatgatta ccaataacca actcaacgtc tataggatta
     1441 gcgcgtgttt ttaaaacatc aatagtttgt ggaaaacaag catcgcttac aaaaaactta
     1501 ttggcatttg ctttttcctg ctctttagtt cgagaagcaa aaaacatgtg tagtgcctca
     1561 gcagctgctg tagcctcgtc aagcaacgat gcattggcaa gctccatacc cgttaaatca
     1621 cataccatag tttggtaatt caaaagcgct tccatacggc cttgcgaaat ttcggcctga
     1681 tagggggtgt atgccgtgta ccaacccggg ttttcaaaaa tattgcgttg aattacaccg
     1741 ggcacaatgg tattataata acccaaaccg atataagtac gatatacttt gtttttcatc
     1801 gaaacccctt ttataaactt taaatatgca gcttcactca tggcttcggg caaatttaaa
     1861 tcgcctttta tacggatcga tttgggaacc gtttggctaa ttaattcttc gattgatgca
     1921 acaccaatag tggttaacat tttggcctcc tgattt
//
LOCUS       DAOMAA010000400         1951 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_59437_length_1951_cov_2.763186, whole genome shotgun sequence.
ACCESSION   DAOMAA010000400 DAOMAA010000000
VERSION     DAOMAA010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59437_length_1951_cov_2.763186"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="PLO59_06025"
     CDS             <1..671
                     /locus_tag="PLO59_06025"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HQV00697.1"
                     /translation="HAQSTFRGEQPAVITSNDSQAKIMVAIDGNNFNAYQPFTLNKST
                     TVNYYAVNGSDSSAVITSKLLQIPDNRQIKMQAVYDNQYTASGNDALIDGLHGDTNWR
                     KGYWQGYANKTLEAIVDLGKSTFVSEVSLGCLQDVGPWIFYPSSIMFAYSNDAVAYKS
                     LPEIIPQAPQANEAISTSNFKAELMATCRYIKVIAKPFGPMPAWHISAGEPAWLFVDE
                     INIK"
     gene            897..1829
                     /locus_tag="PLO59_06030"
     CDS             897..1829
                     /locus_tag="PLO59_06030"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV00698.1"
                     /translation="MKKLLLLSLILGLQHIVFAQVNLVPNPSFEDTVACPNSTNQVDR
                     AVGWYPSRNSPDYFNACDWINGLLGVPNNTAGYQHPQSGDAYCGFIAYLRNDPNYREN
                     FTCSLLNSLIIGRKYKVSFYASWSGKYRLACNKIGALFSTVNYDSLNNSPICNYSHLY
                     TDSIIVDSVNWVLVEGSFIADSNYTYLSIGNFFDSALVDTIYPLPSSIAYYYIDDVSV
                     SEDTTSTILEVPCGGDAITYFPNPVKDKLYACTDTNSLFNIEIINTVGILMLYCKAKS
                     INTEIDLSALESGIYYLKLNFKNKSSSMHKLIKL"
BASE COUNT          648 a          347 c          338 g          618 t
ORIGIN      
        1 tgcatgcaca aagtactttt agaggcgaac aacctgccgt aattacaagc aatgatagcc
       61 aagctaaaat tatggtagct attgatggaa acaactttaa tgcttatcaa ccttttacct
      121 taaataaatc taccaccgta aactactacg ctgttaacgg tagcgacagt agcgcagtta
      181 taacttctaa gttgttgcaa atacccgata accggcaaat aaaaatgcaa gccgtttacg
      241 acaatcaata cactgcaagc ggtaacgatg cattaataga tggcctgcat ggtgatacaa
      301 actggcgtaa aggctattgg caaggttatg caaataaaac attagaagct attgttgact
      361 taggcaaaag cacctttgta agcgaagttt cattaggatg tttgcaagat gttggcccat
      421 ggatttttta tccttcttcc ataatgtttg catacagcaa tgatgctgtt gcctataagt
      481 cattgcccga aataatacca caagcgccac aagcaaatga agccattagc accagtaatt
      541 ttaaggccga actaatggca acttgccgtt atattaaagt aattgccaaa ccttttggcc
      601 ctatgcctgc atggcatata agtgccggtg aaccggcatg gctttttgta gatgaaataa
      661 atatcaagta atacagttgt aacactgact ttaagaaaaa tattcttaca aaactcattg
      721 ctgtttttaa gaaagataat catttcgatt cggcaaacag ttatatgact aacaataaat
      781 agctatgcat taaaaaatgt tcatgatagg ctacgcctca ctaatcacca gcggctaaat
      841 catttaaatc atcggtacac acattttatt tacctttaac agtaaataat atgtctatga
      901 aaaaattact gcttttatcg ttaatacttg gtttgcaaca tattgttttt gcacaagtaa
      961 accttgtacc aaaccccagt tttgaagata cggtagcttg cccaaatagt accaatcaag
     1021 ttgacagagc tgtaggttgg tatcccagca gaaactcacc tgattatttt aacgcttgtg
     1081 attggattaa tggcttgctt ggagtgccaa ataatactgc aggctatcaa cacccacagt
     1141 caggagatgc gtattgcggt tttattgctt atttgcgtaa tgaccccaat taccgtgaaa
     1201 attttacttg ctcattatta aattcattaa taataggaag aaaatacaaa gtgtcctttt
     1261 acgcaagctg gtcaggaaag tatcgtttgg cttgtaataa gataggggca ttgtttagca
     1321 ctgtaaacta tgattcgctt aataactcac caatttgtaa ttactcacat ctttataccg
     1381 atagtataat tgttgattca gttaactggg tattggttga gggtagtttt attgctgact
     1441 ccaattacac ttatttgtct attggaaatt tttttgactc tgcactagta gataccatat
     1501 atcctttgcc atcttctatt gcttattatt atattgatga tgtatctgtt tcagaggata
     1561 ctacttccac aattttggaa gtaccttgcg gaggcgatgc gataacctat ttccctaatc
     1621 ctgttaagga taaactatat gcatgtacgg atacaaatag tttattcaat attgaaatta
     1681 ttaatacggt aggcatattg atgctatatt gtaaagctaa gtccatcaat accgaaatag
     1741 atttatcagc attagaatct ggtatttatt atttgaaact aaatttcaaa aataaaagct
     1801 caagtatgca taaactaata aaattataaa atcatgaaaa aggaattgtt tttaactata
     1861 ttaaacattg cactaacttg cactagtatt ttcggacaag caacaacagc tccaagtaac
     1921 aattggttag gaggacttga atacttaggc t
//
LOCUS       DAOMAA010000401         1950 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_59512_length_1950_cov_2.397889, whole genome shotgun sequence.
ACCESSION   DAOMAA010000401 DAOMAA010000000
VERSION     DAOMAA010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59512_length_1950_cov_2.397889"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="PLO59_06035"
     CDS             complement(<1..252)
                     /locus_tag="PLO59_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004570964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonate 8-phosphate
                     phosphatase"
                     /protein_id="HQV00699.1"
                     /translation="MQNFKEKLAKISCFIFDVDGVLTNGQLIIMPDGELLRSMNIKDG
                     YAMQLAIKKGYKIAIISGGKSEGVPQRLQRLGITEIYMQT"
     gene            complement(257..1030)
                     /locus_tag="PLO59_06040"
     CDS             complement(257..1030)
                     /locus_tag="PLO59_06040"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HQV00700.1"
                     /translation="MTIGIIGSGNVANALAIAFAKNNVVVSAIIARNEITGNKLAQKA
                     KASFYKFPINKLPKADVFLICVSDNALQTVIESLPRSSMLIAHTAGSVGLNLLAGKYK
                     NAGVFYPLQTLKFANKNFKKIPICIEASNQVSENKLLKLASSISNKNIKLNSQQRLHL
                     HLAAVITNNFTNHLYAQAHAYLEAHELPFQLLHPLIEKTAEMVFNTPPNKLQTGPAVR
                     YDANTIKKQIALLDDNEALKKLYQLFTASIQKMNPKKQS"
     gene            complement(1030..>1950)
                     /locus_tag="PLO59_06045"
     CDS             complement(1030..>1950)
                     /locus_tag="PLO59_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00701.1"
                     /translation="KGNIKHSGASIAHVGVGLLLLGALISQSKQEVISQNVNGIDLGK
                     DFPNNENIMLTKGDTLPMGNYYITYTGHKQEGIHIYYGIDYLKLNKTNGLFEKQFELK
                     PTIQLNERMGDAAEPDTKHFIDKDLYTHVTYAELDKNKNQTGDSIYNEAKSVTIKQGD
                     TITTSNSLLVLEGLSTAVDKEKMSLGADDIAVAANLLSIDVNNQKHYANPLYIIKDQM
                     QYSEDAEIDSLGLKISFVKIDTEKNAIELKIAEKKSNKRDFVILKAVVFPHINILWMG
                     CIIMVIGTVIAMRKRFADLKFSNNNAAKVN"
BASE COUNT          550 a          340 c          332 g          728 t
ORIGIN      
        1 ggtttgcata tatatttctg taatgcctaa gcgctgtaaa cgctgtggca cgccttctga
       61 tttacctcct gaaataatag ctattttata acctttttta atggccaatt gcatggcata
      121 accatcttta atattcatac ttcgtaacaa ttcgccatcg ggcataatta ttaactgacc
      181 attggtaagc acaccatcca catcaaatat aaaacagctt atttttgcta acttctcttt
      241 aaagttttgc attggattat gattgttttt taggattcat tttttggatt gaagctgtaa
      301 acaattgata caactttttt aaagcttcat tatcatcaag caaagcaatt tgttttttta
      361 ttgtatttgc atcataccta acggcaggcc cggtttgtaa tttattgggt ggggtattaa
      421 aaaccatttc agctgttttt tcaataagtg gatgcaataa ttgaaatggt agctcatgtg
      481 cttctaaata tgcatgcgcc tgcgcatata aatggttggt aaaattatta gttattacgg
      541 cagccagatg taaatgtaaa cgctgttgcg agtttagttt aatgttttta ttacttatag
      601 acgaagccag tttcagtaat ttattttctg aaacttggtt ggaagcttcg atgcagatgg
      661 gtattttttt aaaattttta ttggcaaatt ttaaagtttg aagcgggtaa aatacaccgg
      721 catttttgta ttttcctgct aacaaattta agccaacgct gccagctgta tgtgcaataa
      781 gcatactgct gcgcggcaga ctttcgataa ccgtttgcaa agcattgtcg gatacgcaaa
      841 ttaaaaacac atcggcctta ggtaatttat ttatgggaaa tttataaaac gatgctttgg
      901 ctttttgtgc taacttgtta cctgttattt cgtttcgggc aataattgcg cttacaacaa
      961 cattgttttt tgcaaatgca attgccagtg catttgccac attacctgag ccaataatgc
     1021 ctatagtcat taatttactt ttgctgcatt attattactg aatttcaaat cggcaaatct
     1081 ttttctcatg gcaattacgg ttccgataac cataattatg catcccatcc ataaaatatt
     1141 tatatgcgga aaaacaactg ctttaagaat tacaaagtct ctcttattcg atttcttttc
     1201 tgcaattttt aattcgatgg catttttttc ggtatcaatt tttacaaacg atatttttag
     1261 tcccaggcta tcaatttcag catcttcgct gtattgcatt tggtctttga taatgtataa
     1321 ggggttggca taatgttttt gattatttac atcaatgctt agcaggtttg ctgcaacagc
     1381 aatatcatca gcacctaagc tcattttttc tttatccaca gcggttgata agccttctaa
     1441 cacaagtaag ctattactgg tggtaatagt atcgccttgc ttaatggtaa ccgatttggc
     1501 ttcgttgtaa atgctatcac cggtttggtt tttgttttta tctaactcgg cataggttac
     1561 atgggtatat aaatctttat caataaaatg tttggtatcg ggttcggcag catctcccat
     1621 acgctcatta agttgtatgg taggttttaa ctcaaattgt ttttcaaaaa gcccattggt
     1681 tttattcaac ttcaggtaat caatgccata gtaaatgtgt atgccttctt gtttatgtcc
     1741 tgtgtaagta atatagtaat tacccatggg taaagtatcg cctttggtaa gcataatgtt
     1801 ttcattattc ggaaaatctt tacctaaatc aataccattt acattttgtg aaataacctc
     1861 ttgtttcgat tgtgaaatta aagcacctag caataataag cccacaccta catgtgctat
     1921 tgatgcgcca ctgtgtttaa tattgccttt
//
LOCUS       DAOMAA010000402         1948 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_59657_length_1948_cov_1.884311, whole genome shotgun sequence.
ACCESSION   DAOMAA010000402 DAOMAA010000000
VERSION     DAOMAA010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59657_length_1948_cov_1.884311"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>213
                     /locus_tag="PLO59_06050"
     CDS             <1..>213
                     /locus_tag="PLO59_06050"
                     /inference="COORDINATES: protein motif:HMM:NF016993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaI"
                     /protein_id="HQV00702.1"
                     /translation="LYQKLTKSSDETLNGFAQKSLKEMIYHLRHASDWMLRLGDGTPE
                     SKQRVQESLDACWLFTDDFFDADAVDA"
     assembly_gap    214..223
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            340..846
                     /gene="paaJ"
                     /locus_tag="PLO59_06055"
     CDS             340..846
                     /gene="paaJ"
                     /locus_tag="PLO59_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020404019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaJ"
                     /protein_id="HQV00703.1"
                     /translation="MPVPAQHTEQSILQLLDAVFDPEVPVLTIAELGVLRKVTLLDNV
                     FNIVITPTYSGCPAMQTIAADITAVMETNGVFNFKITTIIAPPWTTDWMSDTAKQKLK
                     AYGIAPPTNTTANHLASILNRKKSPTTCPFCNATDTKLTSAFGSTACKALHYCNHCQQ
                     PFEEFKCH"
     gene            889..1662
                     /locus_tag="PLO59_06060"
     CDS             889..1662
                     /locus_tag="PLO59_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HQV00704.1"
                     /translation="MTAILYNKQAGIASIILNRPDKFNSFNGPMAMQMQDALTDAATD
                     KNIRCIFISANGKAFCAGQDLSEAIAPTGPGIENIVKKHYNPIVKLLRKTEKPIVCAV
                     NGVAAGAGANIALACDVVVAAQSASFIQAFSKIGLIPDSGGTFTLPRLVGFQRASALM
                     MLGDKVTAADALQMGMLYAVYADEMLQEKAMQLAQTLSQMPTLGIGLTKRLLNMSDNN
                     LQQQLDAEAQMQVIAAQSHDYGEGVNAFLEKRKPVFKGE"
     gene            1713..>1948
                     /locus_tag="PLO59_06065"
     CDS             1713..>1948
                     /locus_tag="PLO59_06065"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009283189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HQV00705.1"
                     /translation="MKKMFMALCFLSINAIQAQPTFVTDSLNTYIKNAMQQWQIPGMA
                     VAIIKDGKVVLANGCGVANVETKKPVDAYTLFQIA"
BASE COUNT          632 a          404 c          378 g          524 t
ORIGIN      
        1 actatatcaa aaactaacca aaagcagcga tgaaacctta aatggttttg cacaaaaatc
       61 gcttaaagaa atgatttacc atttgcgcca tgcatccgat tggatgttac gcttaggcga
      121 tggaactcca gaaagcaaac aacgtgtaca ggagtcgtta gatgcatgct ggctttttac
      181 cgatgatttt tttgatgccg atgccgtaga tgcnnnnnnn nnnaatggtt ttatgcgcag
      241 cggcagccgt aaaggcaatc ataccgaata tctgggtcat ttactggccg atatgcagtt
      301 tattccgcgt gcatatccgg gtacaaagtg gtaaactgta tgccagtacc tgcacaacat
      361 accgaacaaa gtatattgca gttgttagat gccgtattcg accccgaagt accggtacta
      421 accattgccg agttaggcgt actacgcaaa gtaactttgc tcgataatgt ttttaatatt
      481 gtaataacac ctacttacag tggttgcccg gctatgcaaa ccatagcggc agatattact
      541 gcggtaatgg aaaccaatgg cgttttcaac tttaaaatta ccaccattat tgccccaccg
      601 tggactaccg attggatgag cgatacggcc aaacaaaaat taaaagctta tggcattgcg
      661 cctccgacaa atacaacagc caatcattta gcaagtattt taaatcgtaa aaaatcacct
      721 actacttgcc ctttttgtaa tgccaccgat accaaactaa ccagtgcttt tggcagtaca
      781 gcttgtaaag ctctacacta ttgcaaccac tgccaacaac cattcgaaga atttaagtgc
      841 cattaaaatt ttaaattgtg gcctccatta aacaccctat ttatttttat gacagccata
      901 ctttacaaca agcaagcagg cattgcctcc attattttaa acagacctga taagtttaac
      961 agcttcaacg gccctatggc tatgcaaatg caagatgcat taaccgatgc agctaccgat
     1021 aaaaatatca gatgcatttt tatttcagca aacggcaaag ccttttgtgc cggccaggat
     1081 ttatcagaag ctattgcccc aacaggaccg ggtatcgaaa acatagttaa aaaacactac
     1141 aaccccattg taaagttgtt gcgtaaaacc gaaaaaccaa ttgtatgtgc cgtaaatggt
     1201 gtggcagccg gtgcaggagc caacattgct ttggcatgcg atgtagtggt tgcagcacaa
     1261 tcggcctcat ttattcaggc ttttagtaaa attggactta ttcccgatag tggcggcaca
     1321 tttactttac cacgtttagt aggctttcaa cgggccagtg cattaatgat gttgggcgat
     1381 aaagtaactg ctgccgatgc attacaaatg ggaatgttat acgcagtgta tgccgatgaa
     1441 atgttgcagg aaaaagctat gcaattggcg caaacattat ctcaaatgcc cacattgggt
     1501 attggactta ccaaacgctt attaaacatg agtgataaca acttacaaca acaattagat
     1561 gccgaagcac aaatgcaggt aattgcagcg caaagccacg actacggtga gggcgttaac
     1621 gcatttcttg aaaaacgaaa acctgtattt aaaggcgaat aataatttgt aacttcaatt
     1681 aataactttt acgctaaatt aaaatcaaca ctatgaaaaa aatgtttatg gcattgtgtt
     1741 ttttaagcat taatgcaata caagcacaac caacatttgt aaccgacagc ttaaacacct
     1801 atattaaaaa tgccatgcaa caatggcaaa taccgggtat ggcagtagct ataataaaag
     1861 atggtaaagt agtgctggct aatggttgtg gtgttgctaa tgttgaaaca aaaaaaccag
     1921 ttgatgctta caccctgttt caaatagc
//
LOCUS       DAOMAA010000403         1946 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_59771_length_1946_cov_2.669487, whole genome shotgun sequence.
ACCESSION   DAOMAA010000403 DAOMAA010000000
VERSION     DAOMAA010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59771_length_1946_cov_2.669487"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="PLO59_06070"
     CDS             <1..417
                     /locus_tag="PLO59_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00706.1"
                     /translation="CLIVSLFIGVGCSKTNLSETPEIAIESISPSSAKAYTDSITIIM
                     SYKDGNGDLGENHADAKNLFVTDSRNGLVYPFRLQQLAPANAQVPIQGKVNIVLPRMG
                     LTDSSNAQTFYFSVYVHDRALNKSNVVQTPLVTLLK"
     gene            479..1441
                     /locus_tag="PLO59_06075"
     CDS             479..1441
                     /locus_tag="PLO59_06075"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HQV00707.1"
                     /translation="MSKNQLDFEKPIAVLQEQLDKAKQIAEKGKVDTTNLIIELEQKI
                     VETKTQLYANLTPWQRVQLSRHPDRPYALDYIQALTNGDFIEQFGDRTVKDDKAMIGG
                     FGSINGQTLMFIGQQKGNTTKQRQYRNFGMPNPEGYRKALRLMKLAEKFGKPIITLID
                     TPGAYPGLEAEERGQGEAIARNLLEMAVLKVPVICIIIGEGASGGALGIGIGDKVLML
                     ENTWYSVISPESCSSILWRNWDNKERAADALKLTATDMLTQHLIDGIIAEPLGGAHAN
                     RDEIFNTVKEEILQNLSLLKAINIDNLVEQRIAKFSSMGVYNEA"
     gene            1637..>1946
                     /locus_tag="PLO59_06080"
     CDS             1637..>1946
                     /locus_tag="PLO59_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HQV00708.1"
                     /translation="METPSYNLSNNAEAIKSDILFHLFYTQGRTTTLATMHDWYMAVA
                     YTLRSRLMKNWIDALTHYKDHEKKIVGYLSAEFLMGPHLGNAIVNLDIYQEVSEALTQ
                     L"
BASE COUNT          674 a          382 c          358 g          532 t
ORIGIN      
        1 tgtttaatag taagtttgtt cataggtgtt ggatgtagta aaaccaattt atccgaaaca
       61 cctgaaattg ctattgaaag catttcacct tcttcagcta aagcttatac tgattcaata
      121 accatcataa tgagctacaa agacggcaat ggcgatttag gcgaaaacca tgcagatgca
      181 aaaaatttat ttgtaaccga tagtcgcaac ggattggtgt atccatttcg cttacaacaa
      241 ttagcccccg ccaatgcaca agtacccata caaggtaaag taaacattgt acttccacgg
      301 atgggtttaa ccgacagcag caatgcacaa actttttatt tttcagtata tgtgcacgat
      361 agagccttaa ataaaagcaa tgtagtacaa acacctctag ttaccctgct aaagtaaggt
      421 tgctattttt atggtatcag gctaatacta tatttgccca ccttttacaa ataaaaatat
      481 gagtaaaaat caactcgatt tcgaaaaacc gatagcagtt ttgcaggaac aattagacaa
      541 agccaagcaa attgccgaaa aaggaaaagt agatactacc aacttaatta tagagttgga
      601 acaaaaaatt gttgaaacca aaacacaact ctacgctaat ttaacaccgt ggcaacgggt
      661 gcagttatca cgtcatcccg ataggcccta tgcattagac tacatacaag cccttaccaa
      721 tggcgatttt atcgaacaat ttggcgatag aaccgttaaa gatgataaag ccatgatagg
      781 tggttttgga agtattaacg gccaaacatt aatgtttatc ggtcaacaaa aaggcaatac
      841 caccaaacag cgtcaatacc gcaattttgg tatgccaaac cccgaaggct atcgcaaagc
      901 attacgactg atgaaattag ccgaaaaatt tggtaaacca attattacac ttattgatac
      961 accgggcgcc tatccgggct tagaggccga agaacgaggc cagggcgagg ccattgcacg
     1021 taatttgctc gaaatggctg tattaaaagt acccgttata tgtataatca taggcgaagg
     1081 tgcttcggga ggggctttgg gtataggcat tggcgataaa gtattgatgc ttgaaaacac
     1141 ctggtactcc gtaatttctc ccgaatcttg ctcctctatt ttatggcgta actgggataa
     1201 taaagagcgt gctgctgatg cgttaaaact aactgcaacc gatatgctga cgcaacattt
     1261 gattgatggc attattgccg aaccattagg cggtgcacat gccaatcgtg acgaaatatt
     1321 taacaccgtt aaagaagaaa tacttcaaaa cctaagtttg ttaaaagcta ttaatattga
     1381 taacctggtt gaacaacgta ttgccaagtt ttcgtcaatg ggcgtttata acgaagcctg
     1441 atatgcaatt gtaaaatgta aaaacaaaaa agctttgcct taaatgcaag gcttttttta
     1501 gattttatat tatccccccc tttaaataaa aatacaaaac aatattgcgc taacaaacca
     1561 tacctaacat ggttatacaa aagctgctaa tttaatttca actaaatttt ataataaaaa
     1621 aacctgctaa tcaattttgg aaacaccatc gtacaacctt agcaataatg ccgaagcaat
     1681 aaaatcagac attttatttc acttattcta cacacaaggt cgcaccacaa cattggctac
     1741 gatgcacgat tggtacatgg ccgttgctta taccctacgc agtcgattaa tgaaaaactg
     1801 gattgatgca ttgacgcatt acaaagacca tgagaaaaaa attgtaggtt atttatcagc
     1861 cgagttttta atggggccac acctgggtaa tgccatcgtt aatttagata tctatcagga
     1921 ggtaagtgaa gcactaaccc aattag
//
LOCUS       DAOMAA010000404         1944 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_59874_length_1944_cov_2.893065, whole genome shotgun sequence.
ACCESSION   DAOMAA010000404 DAOMAA010000000
VERSION     DAOMAA010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59874_length_1944_cov_2.893065"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1348)
                     /locus_tag="PLO59_06085"
     CDS             complement(<1..1348)
                     /locus_tag="PLO59_06085"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HQV00709.1"
                     /translation="MFKKYLLLILGLLVFETLYAQNALLKGVLIDEEHNTIITKATVS
                     LRGTSYVAEVNANGYFEIKELPYGSYTLLIEANAFIDYNKDVVINKAVVDLGNLALQH
                     ANPQETTQDIPVVTLTESDLKDNGSQNVASSLSASRDAFLSAITFSFSVARFQARGYD
                     NDNVPTYMNGAPMQDLTSGRTQFFTWTGLNDVMRGRENTYGLAAGTYAFGGVGGSFFI
                     DSRASHQRKQFQVSFAKGNRSYDNRLMATYGSGLLKGGWSVAATFSRRWADEGYVKGT
                     FYDGTSYFLSVEKIINNKHFLSLTGFGAPVTNGRTSPAVQEMLDLAGTNYYNANWGYQ
                     NGKVRNVSVAQNHLPTAILSWEWKPTPTSNLMTAISTVNGKSKVSAFDWFNATDPRAD
                     FYRKLPSFIEDSATAAQVAQLLRDNEAERQIKWDELYHTNYNSIATIYNVDGIAGNN"
     gene            1511..>1944
                     /locus_tag="PLO59_06090"
     CDS             1511..>1944
                     /locus_tag="PLO59_06090"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HQV00710.1"
                     /translation="MLLRNCLLFFGFFLVLIASGQDKKYKVAVIGFYNLENLYDTIND
                     PLVNDEEFTPEGQKNYTPKVYLDKLEHLSDVLSQIGTDYTPDGAAIMGVAEVENEQVL
                     KDLAQTHKLKKRNWQTVHYHSPDERGVDVAMLYNPKYFKPLY"
BASE COUNT          591 a          382 c          387 g          584 t
ORIGIN      
        1 tgttatttcc ggcaatgcca tcaacattat aaatggtggc aatgctgtta tagttggtgt
       61 gatataattc atcccattta atttgacgct cggcttcgtt atcgcgtaac agttgcgcaa
      121 cctgagcagc cgttgcactg tcttcaataa aactgggcaa tttgcgataa aaatcggctc
      181 gcggatcggt tgcattaaac cagtcgaaag cagatacttt acttttaccg ttaacggtgc
      241 ttatggcagt catcaggttt gatgttgggg ttggtttcca ttcccaactt aatatggctg
      301 taggtaagtg attttgtgct acacttacgt tacgcacttt gccgttttga taaccccagt
      361 tggcattgta atagttagtg ccggcaaggt cgagcatttc ttgtacagcg ggcgatgtac
      421 ggccattagt taccggtgcg ccaaagcccg taagcgaaag aaagtgtttg ttgtttatta
      481 ttttttcaac ggataagaag taagaagtac catcataaaa agtacctttt acataacctt
      541 cgtcagccca cctgcgcgaa aaagttgcag ccacgctcca accacctttt aacaaaccgc
      601 taccgtatgt agccattaaa cgattgtcgt agctacggtt gcctttggca aaactcactt
      661 gaaattgttt gcgttgatgt gaggcgcgcg agtcaataaa aaacgatccg ccaacaccac
      721 caaatgcata cgtgcctgcg gccaagccat acgtattttc gcggccacgc attacatcat
      781 ttaaccctgt ccaggtaaaa aattgtgtac gtcccgaggt taagtcctgc atgggtgcgc
      841 cattcatata cgtgggtacg ttatcattat catagccacg tgcctgaaaa cgtgccactg
      901 aaaacgaaaa agtaatggcc gataaaaatg catcacgact tgccgataaa ctgcttgcaa
      961 cgttttgcga accattgtct tttaaatcgc tttcggtgag ggtaactacc ggtatatctt
     1021 gtgtggtttc ttgtgggtta gcatgttgta aagccaaatt acctaaatca accacagctt
     1081 tatttataac cacatcttta ttataatcaa taaaagcatt ggcttcaata agcaaggtat
     1141 agctaccgta aggcaactct tttatttcaa aataaccatt ggcatttact tcggcaacat
     1201 aggatgtgcc ccttaaacta acggtggctt tggtaataat cgtgttatgt tcttcgtcta
     1261 tcaaaacgcc ttttaacaat gcattttgcg catatagggt ttcaaaaacc aaaagcccta
     1321 aaattaatag caagtatttt ttaaacataa attcaaatca gttaaatggg cggcaaagta
     1381 atattaaata tggttaccaa aaccttaata aaacaagatg atattgataa gaattagcag
     1441 ctgccagatt atacaaaaat tttttagttc aatgtaatgg cttttatatg tttgctgcaa
     1501 atacctaaat atgttgttac gtaattgttt attgtttttt ggattttttc tggtactgat
     1561 tgctagtggc caggataaaa agtataaggt agccgttatc ggcttttata atttagaaaa
     1621 cttatacgat acgattaatg atccgttggt aaatgatgaa gagtttacac ccgaaggtca
     1681 aaaaaattat accccaaaag tatatctgga taagttggag catttatcag atgtcctatc
     1741 acaaatagga accgactata cacccgatgg tgctgcaata atgggcgttg ccgaagttga
     1801 aaatgagcaa gtacttaaag atttggcgca aacacataaa cttaaaaaac gaaactggca
     1861 aaccgtgcat taccattcgc ctgacgaacg tggtgtggat gtggctatgt tgtacaaccc
     1921 aaaatatttt aaaccacttt atag
//
LOCUS       DAOMAA010000405         1941 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_60069_length_1941_cov_2.857900, whole genome shotgun sequence.
ACCESSION   DAOMAA010000405 DAOMAA010000000
VERSION     DAOMAA010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60069_length_1941_cov_2.857900"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(225..>1941)
                     /locus_tag="PLO59_06095"
     CDS             complement(225..>1941)
                     /locus_tag="PLO59_06095"
                     /inference="COORDINATES: protein motif:HMM:NF027047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="interleukin-like EMT inducer domain-containing
                     protein"
                     /protein_id="HQV00711.1"
                     /translation="CRSSREDKYFIFQTGNATQMDSLTKLFNKLPVGTFVLIYNFGPV
                     SYSTLSPAVKQTLVSLGSDSINTLQDNQAWILFSQVGNTTMAHEITSTQPNQRISLID
                     SLGGNWNTGFSVSEKIGPAQSWQSLQWQITTESTDTALLQLWGVDTLQQQKILIDSIY
                     PQTSIINNLSQIVDAKKYPSLFFKLYTHDTSVVPDPAQMVKWQLLYTPLPEAMVNPAL
                     HYTISNVKPMQGDSVKVTMAIQNISTVAMDSMLVDFYIYNASNQKINLPSKRYKPLTG
                     NDTLHCSIVFSTTHLAGKNQLWIEANPRNDQAEQFHFNNYAQVNFEVSTDITNPLLDV
                     TFDGIHILNGDIVSAKPQIQISLKDENPYLLLNDTNDFVVFIYETNKPFDKKRLYFEY
                     APGSITHQALMQWQPAVDNKNKFNINYAPFLNSGNYTLEVTAQDRSANQSGSRYTINF
                     EVDTTATITELINYPNPFSTSTRFVFTITGSELPQYFQIQIITISGKIVREISKEELG
                     PLHIGRNITTYAWDGKDQFGDKLANGVYLYRIRTQLNGAAIQKRNTEADSYFKNGWGK
                     MYLLR"
BASE COUNT          540 a          361 c          411 g          629 t
ORIGIN      
        1 cccaaagccc ctgaaatgtt tataggaaag tgaaaaataa aaacagcaat aaatgcccac
       61 caacggccgg catctaaatt gggcatatag gcattgccat acataggaag gcaatgtagc
      121 agaataattt tttaaatatt aaaagtcata tatctgaaca tggcggcaaa cacgcataca
      181 acttttatta ataaccgggt atgttgctgc atacaaaaaa ctacttacct gagtaggtac
      241 attttacccc agccgttttt aaaatagcta tcggcttcgg tgtttcgttt ttgtatggct
      301 gcaccattaa gttgtgtacg aatgcggtac agataaacac cgtttgccag cttatcgcca
      361 aactgatctt taccatccca ggcgtatgtg gtaatgttgc ggccaatatg caaggggccc
      421 aactcctcct tacttatttc gcgcactatt ttgccgctta tagtaattat ttgaatttga
      481 aaatactgtg gcaactcact accggttatg gtaaaaacaa aacgtgttga tgtgctaaac
      541 ggattggggt agttaattaa ttcagttata gtggctgtgg tatcaacttc gaaatttatg
      601 gtgtacctgc tgcccgattg gtttgccgac ctgtcttggg ccgtaacttc taaagtgtaa
      661 ttacccgagt ttaaaaaggg tgcatagtta atgttgaatt tgtttttatt gtcaacagca
      721 ggctgccact gcattaatgc ctgatgcgtt atgctgccgg gagcatattc gaaatacaat
      781 cgttttttat cgaagggttt gttggtttcg taaataaaca ctacaaaatc gttggtatcg
      841 ttaagtagta aatatgggtt ttcatctttt aacgaaatct gtatttgtgg tttagccgaa
      901 acgatatcgc catttaatat atgaatgcca tcaaaagtta cgtccaatag cggattggtt
      961 atatccgtac ttacttcaaa atttacttgt gcatagttat taaaatgaaa ttgttcggcc
     1021 tggtcgttac gggggtttgc ttcaatccat aactggtttt tgccggcaag atgtgtagtg
     1081 ctaaaaacaa tgctgcaatg taacgtatcg tttccggtta agggcttgta gcgctttgat
     1141 ggcaaattga ttttttgatt tgatgcatta taaatataaa aatcaaccaa catactatcc
     1201 atagctacgg tacttatatt ttgaatggcc atggtaacct taaccgaatc gccttgcatg
     1261 ggtttaacat tgctaatggt atagtgcaat gcagggttaa ccatggcttc gggcagcggt
     1321 gtgtaaagta attgccactt taccatttgt gccgggtcgg gcactaccga tgtgtcgtgt
     1381 gtgtatagtt taaaaaacaa cgaaggatat tttttggcat caactatttg gctcaggtta
     1441 ttgatgatgc ttgtttgcgg atagattgaa tcgatcagta ttttttgttg ttgtaacgta
     1501 tcaacacccc ataattgcag cagtgccgta tcggtacttt cggtagtaat ttgccattgc
     1561 agcgattgcc aactttgagc agggccaatt ttttcggaaa cggaaaatcc ggtattccaa
     1621 ttaccgccca atgaatcaat aaggctaata cgctggttgg gttgtgtgct ggttatttca
     1681 tgtgccattg ttgtattgcc aacctggcta aaaagtatcc atgcctgatt atcttgcagt
     1741 gtattaatac tatcgcttcc aaggcttacc aacgtttgtt taacagccgg acttaaagta
     1801 ctgtagctaa ctggcccaaa attgtaaatc aatacaaatg tgcccaccgg tagtttatta
     1861 aacagcttgg ttaagctatc catttgtgtg gcattgccgg tttgaaatat aaagtacttg
     1921 tcttctctgc tgcttcggca t
//
LOCUS       DAOMAA010000406         1939 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_60180_length_1939_cov_3.091826, whole genome shotgun sequence.
ACCESSION   DAOMAA010000406 DAOMAA010000000
VERSION     DAOMAA010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60180_length_1939_cov_3.091826"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="PLO59_06100"
     CDS             <1..389
                     /locus_tag="PLO59_06100"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HQV00712.1"
                     /translation="LGPTIYLRCQYVITEIARVQLAIEALTTQDFNRLGALLTQTHQG
                     LSAQYAVSCPELDFLVETAINCKGVYGARMMGGGFGGCTINLIQNNEVETVSNAVQKQ
                     YADTFGIQMKIYHLNIAEGVHQYFIP"
     gene            386..1447
                     /locus_tag="PLO59_06105"
     CDS             386..1447
                     /locus_tag="PLO59_06105"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HQV00713.1"
                     /translation="MSNPFDSTKHPHRRYNPLLDEWILVSPQRATRPWQGQQETIAQP
                     APKAHDPNCYLCAGNQRANGSNNPNYEGVFVFENDFAALLKDEVTWQNQANTFFKLMP
                     ERGINKVICFSENHGITMPEMEITAIINVVNAWQQQYLQLSALEYINYIQIFENKGSV
                     MGCSNPHPHGQIWAQSSIPTQVAKTQLQFQKYYQQHHTSLLADYLANETEAAQRIVLE
                     NNSFVALVPFWAVWPYETLIISKRKTETILDFSDKEKTDFALILKQLTTKYDNLFSTS
                     FPYSAGIHQAPCDGKPHPEWHFHMHFYPPLLRSASIKKFMVGYEMLAEAQRDITAEQS
                     ADILKNLPIIHYKNKTTRE"
     gene            1440..>1939
                     /locus_tag="PLO59_06110"
     CDS             1440..>1939
                     /locus_tag="PLO59_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017259007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium transporter"
                     /protein_id="HQV00714.1"
                     /translation="MNKLSTPDYIVFALYFIVIVVYGIYIFRKKKNEHADSKDYFLAE
                     GSLTWWAIGASLIASNISAEQFIGMSGSGFSMGLAIATYEWMAAFTLIIVAVFFLPVY
                     IKNNIYTMPQFLAQRYNTKVALIMSVFWLLLYVVVNLTSILYLGALALTSISGINISV
                     AMIFLSV"
BASE COUNT          622 a          413 c          362 g          542 t
ORIGIN      
        1 aacttggtcc aactatatac ctacggtgcc aatatgtgat aacagaaata gcacgtgtac
       61 agttagccat tgaagcactt accacacagg actttaaccg gttgggtgct ttgctaacac
      121 aaacacatca gggcctatct gcgcaatatg cagtgagttg ccccgagttg gattttttag
      181 tagaaactgc cattaattgc aaaggtgtat atggtgcacg catgatgggt ggcggatttg
      241 gtggttgcac tatcaatctg atacaaaaca atgaagtaga aaccgtttcg aatgcagtac
      301 aaaaacagta tgccgataca tttggcattc agatgaaaat atatcacctg aacattgccg
      361 aaggcgttca ccaatatttt ataccatgag caatccattc gactcaacca aacatcctca
      421 ccgaaggtat aacccactat tagatgaatg gatacttgta tcgccacaaa gagccacccg
      481 accctggcaa gggcaacaag aaaccattgc ccagcctgcg ccaaaagccc acgaccccaa
      541 ctgctattta tgtgcaggaa accagcgtgc caatggcagc aacaatccaa actacgaagg
      601 tgtatttgtt tttgaaaacg attttgctgc acttttaaaa gatgaagtaa catggcaaaa
      661 ccaagccaat actttcttta aacttatgcc cgagcgcggt ataaacaaag taatatgctt
      721 ttccgaaaat catggcatca ccatgcccga aatggaaata acagccatta taaacgtagt
      781 taatgcctgg caacaacagt acctgcaatt aagcgcactt gaatacatca actacattca
      841 gattttcgaa aacaaaggca gcgtaatggg atgcagtaat ccacatccgc atggacaaat
      901 ttgggcgcaa tcgtccatac ctacacaagt tgctaaaacc caattgcaat ttcaaaaata
      961 ttaccaacaa caccacacat cgttgcttgc tgattattta gcaaacgaaa ccgaagcagc
     1021 ccaacgcatt gttttagaaa acaattcatt tgtagcctta gttccttttt gggctgtatg
     1081 gccttatgaa acacttatta tcagcaaaag aaaaactgaa accattttag acttttcgga
     1141 caaggagaaa acggactttg ctttaatatt gaaacagctt acaactaaat acgataatct
     1201 ttttagcacc tcatttccat attcggcagg catacaccag gcaccatgcg atggcaaacc
     1261 acatccggag tggcattttc acatgcactt ttatccacca ttattacggt cggcttcgat
     1321 aaaaaaattt atggtgggat atgaaatgct tgccgaagca caaagagata ttactgccga
     1381 acaaagtgcc gatatattaa aaaacctacc catcattcac tataaaaaca aaaccacacg
     1441 tgaataaatt aagtactcct gattatattg tttttgctct atacttcata gtaatagtag
     1501 tatatggcat ttacattttc cgaaaaaaga aaaatgaaca tgccgatagt aaagattatt
     1561 ttttggccga aggttcgctt acatggtggg ctattggcgc ttccttaatt gccagtaaca
     1621 tcagtgccga gcaatttatt ggtatgagtg gcagtggctt tagcatgggc ttagctattg
     1681 caacctatga gtggatggct gcttttacac taattattgt ggctgtgttt tttctgccgg
     1741 tgtacattaa aaataatata tacaccatgc cacaatttct ggcgcaacgc tacaatacta
     1801 aggtagcttt aattatgtcg gttttttggt tgttgttgta cgtagttgta aatctaacat
     1861 ctatactata cttaggtgct ttagcgctta caagtatttc gggcatcaac atttctgttg
     1921 ccatgatatt tctgtccgt
//
LOCUS       DAOMAA010000407         1939 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_60183_length_1939_cov_2.983015, whole genome shotgun sequence.
ACCESSION   DAOMAA010000407 DAOMAA010000000
VERSION     DAOMAA010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60183_length_1939_cov_2.983015"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..568)
                     /locus_tag="PLO59_06115"
     CDS             complement(17..568)
                     /locus_tag="PLO59_06115"
                     /inference="COORDINATES: protein motif:HMM:NF021554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HQV00715.1"
                     /translation="MHGINYFKTLLPKMGSVFLLPAIFAITAKAQIASLNKPFAIDVI
                     AESQIHLNDKQSAIGKKHLVIGTGSVLYVGGMAGLYQLWYADYENQKFHFFNDGNEWL
                     QVDKFGHAFTTYQLSRLSNDALLWAGFSHKQACVYGTGYALLFQTTIEVFDGLSSAWG
                     FSWFDMIANATGGGIFAGRWRDG"
     gene            complement(582..>1939)
                     /locus_tag="PLO59_06120"
     CDS             complement(582..>1939)
                     /locus_tag="PLO59_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HQV00716.1"
                     /translation="EFTVNNVTRVAEATANWMIARNKNASVVVGHDCRFAGEMFAHTV
                     AKVCCAKGVKVFLAKDFVSTPMISLGAVNHKTDLGVIITASHNPPSYNGYKLKGSYGG
                     PLTPAQVQEIEDIIPDVPSIDADSLSLADFEKKGLLEYTDLETMYCKHVEANFDLDAI
                     RNSGLRLAYDAMYGAGQNVIKRLLPETIKIHCDYNPSFMGQAPEPIHKNLQEFSNLIK
                     SDGKIDCGLATDGDADRIGLYDGKGNFVDSHHIILLLIHYLVKYKKQSGKVCIAFSVT
                     PRVKKLCDHYGLEMQTLKIGFKWICEVMIKEDVLVGGEESGGIAIKGHIPERDGIWMG
                     LVIWEFMAKSGKNLQQLIDEVYAITGPFAFERVDLHLTEAEKNKAIDKCKSGSLTAFG
                     SYKIAKADDLDGYKYFFENGDWIMIRASGTEPVLRTYAESDTSKAAYDILAAGKQTLL
                     S"
BASE COUNT          564 a          457 c          330 g          588 t
ORIGIN      
        1 tggctcggca tcgacatcac ccatctcgcc atcgccctgc aaaaataccg cctcctgttg
       61 cattggcaat catatcaaac caactaaaac cccatgcact acttaagccg tcaaaaactt
      121 cgatagtagt ttgaaataac aatgcataac ctgtaccata tacgcatgcc tgtttatggc
      181 taaaaccggc ccacaataaa gcgtcattgc ttaatctgct taattgatat gttgtaaatg
      241 catggccaaa tttatcaacc tgaagccact cattgccatc gttaaaaaaa tggaattttt
      301 gattttcata atccgcatac caaagttggt ataagcctgc cataccccct acatataaaa
      361 cactgcctgt gccaataacc aaatgcttct taccaatagc gctttgttta tcgtttaagt
      421 gtatttggct ttctgctatt acatcaattg cgaacggctt atttagtgaa gctatttgtg
      481 cctttgctgt aattgcaaaa attgcaggca ataaaaaaac gctgcccatt tttggtaaca
      541 gcgttttaaa ataatttatg ccatgcatgc taaaaaagca tttaggataa cagcgtttgt
      601 ttgccggcag ctaatatatc gtaagcagct ttactggtat cgctttcggc ataggtacgt
      661 aacaccggtt cggtacccga tgccctgatc attatccagt cgccattttc gaaaaagtat
      721 ttataaccat ctaaatcatc ggccttggca attttataag aaccaaaagc cgttaacgac
      781 ccacttttac atttatcaat agctttgttt ttctcggctt cggtaaggtg taaatcaaca
      841 cgctcaaaag caaacgggcc ggtaattgca tatacttcat caattagttg ttgcaggttt
      901 ttaccgctct tggccataaa ttcccaaata actaacccca tccatatacc atcgcgctcg
      961 ggtatatgtc ccttaatggc aatacctcca ctctcttcgc cacccactaa aacatcttcc
     1021 ttaatcatta cctcgcaaat ccatttaaag ccaattttaa gtgtttgcat ttcgaggcca
     1081 tagtggtcgc acaatttttt tacgcgtggc gtaaccgaaa acgctatgca tactttgcca
     1141 ctttgttttt tgtatttaac caggtagtgt attaacaata aaatgatgtg atgcgaatcc
     1201 acaaaattgc cctttccatc atataagcca atacggtcgg catcgccatc ggttgccaaa
     1261 ccacaatcaa ttttaccatc gcttttaatc agattcgaaa attcttgtaa gtttttatga
     1321 ataggctcgg gtgcctgtcc cataaacgat gggttataat cgcaatgtat tttaatggtt
     1381 tcgggcaaca atcttttaat cacattttgg ccggcaccat acatagcatc ataagccaaa
     1441 cgcaaacccg aatttctgat ggcatctaaa tcgaagtttg cctcaacatg tttacaatac
     1501 atggtttcga ggtcggtgta ttccaataaa cctttctttt caaaatcggc aagcgataaa
     1561 ctgtcagcat caatagatgg cacatcgggt ataatatctt caatttcctg cacctgtgcc
     1621 ggagtaagcg gcccgccata actgcctttt aatttatacc cattgtatga aggcggattg
     1681 tgacttgccg taataattac acccaaatcg gttttgtggt ttacggcacc taatgaaatc
     1741 atgggtgtag aaacaaaatc ttttgccaaa aaaaccttta cccctttggc acaacaaact
     1801 ttagcaacgg tatgtgcaaa catttcgcct gcaaagcggc aatcgtggcc aactaccacc
     1861 gatgcatttt tattgcgggc tatcatccag ttggctgtag cttcggcaac ccttgttacg
     1921 ttatttactg taaactcct
//
LOCUS       DAOMAA010000408         1936 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_60361_length_1936_cov_3.248272, whole genome shotgun sequence.
ACCESSION   DAOMAA010000408 DAOMAA010000000
VERSION     DAOMAA010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60361_length_1936_cov_3.248272"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="PLO59_06125"
     CDS             complement(<1..831)
                     /locus_tag="PLO59_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HQV00717.1"
                     /translation="MQYRYLGKSGLQLSVFSLGSWLTIGKSVDLKFSSKLMHYAYDNG
                     INFFDSAEGYAGGQAEIVMGQILKKAGWERSSYIVSSKVFYGTKGDNNKPNEKGLSRK
                     HIVEACHAALKRMQVDYLDLYLCHRPDPNVPMAEVVFTMNHLMQQGKILYWGTSEWSA
                     AEIMEAHAVARQNNLQGPVVEQPEYNMFVRTKMERDFLPIFKNTGLGTTIWSPLNSGM
                     LTGKYLNGIPKNSRFDTKGLEWLRDKELLDNKLRKIKLLQKLADKTEMQLGQMALAWC
                     L"
     gene            complement(873..1838)
                     /locus_tag="PLO59_06130"
     CDS             complement(873..1838)
                     /locus_tag="PLO59_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020605728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HQV00718.1"
                     /translation="MNRRSFVRKSSLLAAGSLALPQISKSHNPPFSAMPVIVSTWDFG
                     KAPHEAAWNAISKKGSALDACIAGVKVVEADETNHSVGIGGYPDRDGHVTLDACVMNE
                     QGNCGGVFFVEEILHPSELARMVMEKTPHVYLAGQGAVQFALEQGFKKQNLLTTEAKE
                     EWEKWCKQTNYKPRPMDEKNHDTVGMIAIDKLGNLSGCCSTSGWAYKMHGRVGDSPNI
                     GSGLFVDNEIGAATSTGLGEYIIKICGAHLIVESMRNGRTPQEACEAACLRISSKYKN
                     YKDFQACFIALGKDGTVGAYSLNKGFQYTVTTNGETLVYDAEYLE"
BASE COUNT          494 a          429 c          376 g          637 t
ORIGIN      
        1 taaacaccag gccaatgcca tttgtcctaa ttgcatttcg gttttgtcgg ccagtttttg
       61 taatagtttt atttttctga gtttgttgtc gagtaattct ttatcgcgca gccattctaa
      121 acctttggta tcgaaacgtg aatttttagg tattccgttt aaatattttc cggttaacat
      181 gcctgagttt agcggactcc aaatggttgt acccaagccc gtgtttttaa aaatgggcaa
      241 aaaatcgcgc tccattttgg ttcgtacaaa catgttgtat tcgggttgtt ctactacagg
      301 gccttgtaaa ttattttgac gtgcaacggc atgcgcttcc ataatttcgg cagcactcca
      361 ctcgctggta ccccagtata atattttgcc ttgctgcatt aaatggttca tggtaaacac
      421 cacttcggcc ataggtacat tggggtcggg gcggtggcat aagtataagt ccaggtaatc
      481 tacctgcata cgttttaatg cagcatggca tgcttctaca atatgtttgc gcgataaacc
      541 tttttcattg ggcttattgt tatcgccttt ggtgccgtaa aaaactttac tggatactat
      601 gtaggagctg cgttcccagc cggctttttt taaaatctgt cccatcacaa tttcagcctg
      661 tccaccggca taaccttcgg cactatcgaa aaagttgatg ccattatcgt atgcataatg
      721 catcaacttt gatgaaaatt taaggtcaac acttttgcca atagtaagcc aactgcctaa
      781 tgaaaataca ctaagttgta aaccgctttt acctaaatat ctgtattgca taaatgggga
      841 tgtgctttca aaaactacgt atataaatca ggttattcca aatactcggc atcataaacc
      901 agtgtttcgc cattggtggt aacggtgtat tgaaaacctt tgtttaaact atatgcaccc
      961 accgtaccat cttttccaag agcaataaag caagcttgaa aatctttata gtttttatat
     1021 tttgaactga tacgcaggca agctgcttcg caagcttctt gtggcgtacg tccattacgc
     1081 atgctttcaa ctattaaatg ggcaccacaa atttttataa tgtattcacc taatcctgtg
     1141 ctggttgcag caccaatttc attatctaca aacaagcccg agccgatgtt gggcgagtcg
     1201 cccacgcgtc catgcatttt atatgcccag ccactggtgc tgcaacatcc cgataggttg
     1261 cctaatttat ctatggcaat catgcctaca gtgtcgtgat ttttttcgtc cattgggcgc
     1321 ggtttgtaat tggtttgttt gcaccatttt tcccactctt ctttagcttc ggtagtaagc
     1381 aggttttgtt ttttaaagcc ttgctctaaa gcaaattgca cggcaccttg gccggctaaa
     1441 taaacatgtg gtgttttttc cattaccata cgagccaatt ctgatggatg caaaatttct
     1501 tccacaaaaa acacaccgcc acaattgcct tgttcattca tcacgcaggc atctaaggtt
     1561 acgtggccat ctctatcggg ataaccgcct atgcctacac tgtgattcgt ttcgtcagct
     1621 tctacaactt ttacacctgc aatgcaagca tccaaggccg agcctttttt gctgatcgca
     1681 ttccatgcag cttcgtgcgg ggctttacca aaatcccagg ttgatacgat aaccggcatt
     1741 gcactaaatg gcgggttatg cgattttgat atttggggta aagctaaact gcctgctgcc
     1801 agcaaactgc ttttacgtac aaacgatcgc ctgttcatag ttgtttcgat ttaggagaga
     1861 tgttattgtt tgatagctaa tcggccttta tgtctttaat atttagctgc aacgttactt
     1921 tattattaaa actgtt
//
LOCUS       DAOMAA010000409         1934 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_60493_length_1934_cov_2.575838, whole genome shotgun sequence.
ACCESSION   DAOMAA010000409 DAOMAA010000000
VERSION     DAOMAA010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60493_length_1934_cov_2.575838"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            344..1204
                     /gene="rfbA"
                     /locus_tag="PLO59_06135"
     CDS             344..1204
                     /gene="rfbA"
                     /locus_tag="PLO59_06135"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013930292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HQV00719.1"
                     /translation="MKGIILAGGSGTRLHPLTLAMSKQLMPVYDKPMIYYPLSVLLMA
                     GINEILIISTPHDLPQFKRLLGDGKTLGCRFEYAEQAIPNGLAQAFVIGQSFIGNDAV
                     ALILGDNIFYGSGMQSLLKNNKQPDGGVVFAYHVSDPERYGVVEFDSGMNAVSIEEKP
                     AKPKSNYAVPGLYFYNNSVCDIAKSLKPSARGEYEITDVNKTYLQQGKLKVGILDKGT
                     AWLDTGTFASLMQASQFVQVIEERQGLKIGCIEEVAFEQGFISKKQLANIAEPLLKSG
                     YGKYLMSLIS"
     gene            1256..>1934
                     /locus_tag="PLO59_06140"
     CDS             1256..>1934
                     /locus_tag="PLO59_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQV00720.1"
                     /translation="MKILVTGGAGFIGSWMAAKLADNAQNQIVIVDNLLTGRTENIPT
                     SKHNNVKFIKADVNDFKDISSIFHSYSFDYVFHYAAVVGVQRTLQHPSWVLNDLTGLK
                     NVLNLSKNTGVKQIYYSSSSEVYGEPVEYPQNEHTTPLNSRLPYAIVKNVGEAYLRAY
                     KQEYDLDFTIFRFFNTYGPGQSTDFVMSKFLNAALKNNDITIYGDGMQTRTFCFILDN
                     IEACYNAF"
BASE COUNT          617 a          388 c          366 g          563 t
ORIGIN      
        1 cctgctaatg gctaattgtt acttttagcg ctctattttg cttttatgta tggtttttta
       61 gcgcatgtta ttctttataa ccgcatcctt tttattattg ccccaatggc aatgccgggt
      121 tttttagtat gccacacaca tgccgaattt ataaatgatt ttgccaagcc tttaccaaca
      181 actacaataa ccaatttaca atgcaatggt atttaaaaag agtagctgtt actatttaac
      241 tgctaactat aagtagaaat gtttattaaa acttagttga tacccaaatt atttcacatc
      301 cataaaaccc ttacaaaaac ctatacacta acagttacaa cacatgaaag gaataatatt
      361 agcaggcggt tcgggcacgc gcttacatcc cttaacatta gccatgagta aacagctaat
      421 gcctgtgtat gataagccca tgatttatta tccgttatcg gtgctgctga tggccggtat
      481 caatgaaata cttattattt cgaccccaca cgatttgccg cagtttaagc gcttgttagg
      541 cgatggcaaa acattaggct gccggttcga atatgccgag caggccatac ccaatggctt
      601 ggcacaggcc tttgttattg gccaatcgtt tataggcaat gatgccgttg ccctgatact
      661 tggcgataat attttttatg gcagtggtat gcaatcatta ctaaaaaaca acaaacaacc
      721 cgatggcggt gtggtgtttg cttatcacgt aagcgacccc gaacgttatg gtgtggttga
      781 gtttgacagc ggcatgaatg ctgtatcaat agaagaaaaa ccggccaaac ccaaatcaaa
      841 ttatgctgta ccgggtttgt atttttataa taacagtgtt tgtgatattg ccaaatcgtt
      901 aaagcccagt gcgcgtggcg agtatgaaat aaccgatgta aacaaaacgt atttgcaaca
      961 aggcaaatta aaagttggaa ttttagataa aggcacagcc tggttagata caggcacctt
     1021 tgcctcgctt atgcaagcat cgcaatttgt gcaggttatt gaagagcggc aagggctaaa
     1081 aattggctgt attgaggagg ttgccttcga acaaggtttt atatctaaaa aacaattggc
     1141 aaacattgcc gaacctttac ttaaaagtgg ctatggcaaa tacctgatga gcctaatcag
     1201 ctaagttttt ataaatttaa aaggctttaa atcaacaaaa gtaaaccaaa caaaaatgaa
     1261 aatattagta acaggcgggg ccggatttat tggaagctgg atggctgcaa aactggccga
     1321 caatgcgcaa aatcaaattg taattgtaga taacttactt acaggccgca ctgaaaatat
     1381 acccacatcc aaacataata acgttaagtt tattaaagcc gatgtaaacg attttaaaga
     1441 cataagcagc atatttcact catactcttt tgattatgtg tttcactatg ctgccgtagt
     1501 aggcgtacaa cgcaccttac aacatccatc atgggtgcta aacgatttaa ccggtttaaa
     1561 aaacgtactt aacctgtcta aaaacacagg cgttaaacaa atttactact catcatcatc
     1621 tgaagtttat ggcgaaccgg tagagtatcc gcaaaacgaa cacactacgc cactaaactc
     1681 gcgcctgcct tatgccattg taaaaaatgt gggcgaagca tacctgcgtg cctacaaaca
     1741 agaatacgat ttagacttta ccattttcag attctttaat acctatggcc cgggtcagag
     1801 taccgatttt gttatgagta aatttttaaa tgctgcctta aaaaataacg acatcaccat
     1861 ttatggcgat ggcatgcaaa cacgtacctt ttgtttcata ctcgacaaca ttgaggcttg
     1921 ttacaatgcc tttg
//
LOCUS       DAOMAA010000410         1933 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_60563_length_1933_cov_2.466986, whole genome shotgun sequence.
ACCESSION   DAOMAA010000410 DAOMAA010000000
VERSION     DAOMAA010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60563_length_1933_cov_2.466986"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="PLO59_06145"
     CDS             <1..949
                     /locus_tag="PLO59_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00721.1"
                     /translation="ENIEKEAATILNDVETNVDDINKDVNSDIHPQHLHDIIEARLAE
                     LAPSETSANKTETIEVAAKIESDISTEQQNQIEDVSVETSSTTINFHSKDVETEAEFL
                     ETDVTATDAQAETNLATHPIYLIETASVADIMLADLKEENVNKISDGTENLTTQNASE
                     KHTFSQWLKQLTKDAQVAEVHANEPLAPFKTNINSSTVIIESTNNNPADSSIHSNSEP
                     KPDSHNSINHLIDKFIDEQPRIEPQKSNFYSPSVAARNSVIFNDSVVSETLALIFEKQ
                     QLYQKAINAYEKLSLMFPEKSDLFAARISVLKQKKGNSH"
     gene            955..1305
                     /gene="secG"
                     /locus_tag="PLO59_06150"
     CDS             955..1305
                     /gene="secG"
                     /locus_tag="PLO59_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005865837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HQV00722.1"
                     /translation="MYLFITILIFLASILLSLVVLIQSSKGGGLAAGLSSSNQVMGVR
                     KTTEGLEKLTWGFGIALMVLVLVATFSLPNSQTSATGTTTTKQSTTLERAAKTNVPQA
                     APAPQQQAAPPASK"
     gene            1530..1808
                     /locus_tag="PLO59_06155"
     CDS             1530..1808
                     /locus_tag="PLO59_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019597634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HQV00723.1"
                     /translation="MSNFNLKPLADRVIVEAAAAEEKTASGIIIPDTAKEKPMQGTVV
                     AVGAGKKDEPMTVKVGDKVLYGKYAGTEISHNGTEYLIMRESDIFAVI"
BASE COUNT          719 a          378 c          332 g          504 t
ORIGIN      
        1 agaaaatatt gaaaaagaag cagcaacaat tttaaatgat gttgagacca atgttgacga
       61 cataaataaa gacgtaaatt cagatatcca tccacaacat ttacacgata ttattgaagc
      121 acgtttggct gagttggcac cctcagaaac atcggctaat aagacagaaa ctattgaagt
      181 tgcagcaaaa attgaatctg acataagtac agaacaacaa aatcaaatag aagatgtatc
      241 agttgaaacc tcatctacaa ctattaattt tcattcgaaa gatgttgaaa cggaggcaga
      301 atttttggaa accgatgtta cagcaacgga tgcgcaagct gaaaccaact tggctaccca
      361 tcctatttat ttgattgaaa cagcatcggt tgcagatata atgttagctg accttaagga
      421 agaaaacgtt aataaaataa gcgatggcac cgaaaactta acaacacaaa atgcttcaga
      481 gaaacacacc tttagccaat ggcttaaaca acttacaaaa gatgcccagg ttgccgaagt
      541 acatgcaaac gaaccgttgg ctccatttaa aaccaacatc aactcaagta ccgtaataat
      601 cgaatcaaca aataacaacc cggccgactc atctattcat agtaattctg aacctaagcc
      661 tgatagccat aattcgataa atcatttaat agataaattt attgatgaac aaccgcgcat
      721 cgaaccacaa aaaagcaact tttactcgcc cagtgtagct gcccgcaaca gcgttatttt
      781 taatgacagt gtagttagcg aaacattggc acttattttt gaaaaacagc aactctatca
      841 aaaggcaata aatgcttatg aaaaattaag tttgatgttt cccgaaaaaa gtgatttatt
      901 tgccgcccga atttcagttt taaaacaaaa aaaaggcaat agccattaaa caacatgtat
      961 ttattcatca caattttaat atttctggca tccatactac ttagtttagt tgtattaatt
     1021 caaagttcaa aaggtggcgg tttagccgca ggccttagtt catcaaatca ggttatgggt
     1081 gtacgcaaaa ccaccgaagg tttagaaaaa ttaacctggg gatttggaat tgctttaatg
     1141 gttttagtat tggtagctac tttttcatta cctaacagcc aaacaagcgc cacaggtaca
     1201 actacaacca aacaatcaac taccttagag cgtgctgcca aaaccaatgt gccacaagct
     1261 gcaccggcac cacaacaaca ggctgcaccg ccagcaagca aataattacg ctaaaaactg
     1321 cttacaaggg cagttttttt ttgcacgtat tttttaaacc gagtttcagc acttagccag
     1381 catggcaatt acttttataa ttaactaaag tattgctgcc aatcttaaag ccgggtattg
     1441 aaattttgtc agtataaacc aaatggcata attactgaca aaggccaacc gaaaattcat
     1501 ataacattaa atataaaatc attaaaacta tgagtaattt taatttaaag ccattagcag
     1561 atcgcgtaat tgttgaagct gcagctgccg aagaaaaaac tgccagcgga attattattc
     1621 ccgatacggc taaagaaaaa ccgatgcaag gcactgttgt agcagtgggc gcaggtaaaa
     1681 aagatgaacc catgaccgtt aaagttggcg acaaagtttt atacggtaaa tatgccggta
     1741 cggaaataag tcataatggt actgaatact taatcatgcg cgaaagcgac atttttgctg
     1801 ttatctaaac aaaaacattt tacaaatgca gcagtttgta atcctcactt taaaaacaaa
     1861 tcatttaaaa caataaaatt atggcaaagg aaatcacatt taacctcgaa gcccgcgatg
     1921 cattaaagcg cgg
//
LOCUS       DAOMAA010000411         1927 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_60959_length_1927_cov_2.388355, whole genome shotgun sequence.
ACCESSION   DAOMAA010000411 DAOMAA010000000
VERSION     DAOMAA010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60959_length_1927_cov_2.388355"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /locus_tag="PLO59_06160"
     CDS             <1..851
                     /locus_tag="PLO59_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00724.1"
                     /translation="HVNAQYEKQGALNLQQFFTFKDVTQHCITQTDWIRQKLIKAKLT
                     LENGKIPAIDIGEHCKLPYPCDFTNTCHRHVSKPLSLVVPTDFNLEPTAKQMVLCMLS
                     TKPAIPVYDGCKPYQLIPFAFATKSTSSRFVHQFTGNNYENPTNLFIEKLYNVLADAD
                     VIWVDEILVATQLFNFFNQMSYADQNWELITEKLAGIDTFFTSYNYFDINLAAQQIQV
                     TVPINTKVPHAIAATEIYMTMQQSQDLFSAAEQAELIQQYLSDQLQLLSKMANTLGRF
                     TNTNYV"
     gene            844..>1927
                     /locus_tag="PLO59_06165"
     CDS             844..>1927
                     /locus_tag="PLO59_06165"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HQV00725.1"
                     /translation="MYKKVILKAGKERSVLNYHPWLFSGAIAKHQAVDSEIVEVFDAQ
                     NNYLATGHFSASSIAVRIITFEKQIIDDNFWVKKIQQAYNTRAILGLTDNAATNAYRL
                     VHAEGDGLPGLIIDIYAQAAVIQCHSKGMYTHLQQIAHALQNVYGKKLQTIYNKSAET
                     LHQNNVSENGFLLGNAASGVITENHIPFEVNWAEGQKTGFFIDQRYNRQLLTHYCKNK
                     SVLNTFAYSGGFSFYAMQAGASVVHSVDSSKKAAATYANNLLLHKTIPTQQYITSDVM
                     DFIKVNEEAYDIIILDPPAFAKHHDAIKKAAIAYRNLNAEAFKQIKPNGILFTFSCSQ
                     AIDKTLFRKIIFAAAAMSKRHIKILHQ"
BASE COUNT          693 a          373 c          321 g          540 t
ORIGIN      
        1 tacatgttaa tgcacagtat gaaaaacaag gggctttaaa cttacagcag ttttttacat
       61 ttaaagatgt tacccagcat tgcattacgc aaaccgattg gatacgccaa aaacttataa
      121 aagcaaagct taccctggaa aacggaaaaa ttccagcaat tgacattggc gaacattgta
      181 aactacccta cccctgcgat tttacaaata cttgtcatag acatgtgagc aaacctttat
      241 cgttggtagt gccaaccgat ttcaatttag aacctacggc caaacaaatg gtattatgca
      301 tgttaagcac caaacctgcc atacctgttt acgatggatg taaaccttat caactcatac
      361 catttgcctt tgcgacaaaa agtacaagca gccgttttgt acatcaattt acaggtaata
      421 actatgaaaa tccaactaac ctttttatag agaaattata taacgtcctt gctgatgcag
      481 atgtaatttg ggtagatgaa attttagttg caacccaact ttttaatttt tttaatcaaa
      541 tgagttatgc cgatcaaaac tgggaattaa ttactgaaaa attagcaggc attgatacat
      601 tctttacaag ctataattat ttcgatatta atttagcagc acaacaaata caagttacag
      661 ttcccataaa tacaaaagtg ccacatgcaa ttgctgctac cgaaatttat atgactatgc
      721 aacaatcgca agatttgttt tcggctgccg aacaagccga actcattcaa caatacttat
      781 cagatcagtt acaactccta tcaaaaatgg ccaatacctt agggcgtttt acaaatacca
      841 actatgtata aaaaagtaat tttaaaagcc gggaaagagc ggtcggtact aaattatcat
      901 ccctggttgt ttagcggtgc cattgcaaaa catcaggccg ttgatagcga aatagttgaa
      961 gtatttgatg ctcaaaataa ttacttggct accgggcact ttagtgcaag cagcattgct
     1021 gtaagaatta tcacattcga aaagcaaata atagacgata atttttgggt taaaaaaata
     1081 caacaagcat ataacacccg tgccatactt ggattaaccg ataatgcggc aacaaatgct
     1141 tatcgtttag tacatgccga gggcgatggc ttgcccggcc ttataattga tatctatgca
     1201 caagcggctg ttatacaatg ccacagtaag ggcatgtaca cacatttgca acaaatagca
     1261 catgcgcttc aaaatgttta tggtaaaaag ctacaaacaa tttataataa aagtgccgaa
     1321 acattacacc aaaacaatgt tagtgaaaat ggatttttat taggcaatgc agcatcaggt
     1381 gttataactg aaaaccatat tccttttgaa gtaaattggg ccgaaggaca aaaaaccggt
     1441 ttctttattg atcaacgtta caaccgacaa ctacttacac actattgtaa aaacaaatcg
     1501 gtgctgaata catttgccta ctcgggaggc ttttcatttt atgcgatgca agccggtgct
     1561 tcagttgttc attcggtaga tagcagtaaa aaagcagcag caacttatgc caataattta
     1621 ttgttgcaca aaaccattcc gacacaacaa tacataacaa gcgatgtaat ggattttata
     1681 aaagtaaatg aagaagctta tgatattata atccttgatc cacctgcatt tgccaaacat
     1741 cacgatgcca taaaaaaagc tgccattgca tatagaaatt taaatgccga agcctttaaa
     1801 caaattaaac caaatgggat attattcaca ttcagttgct cacaagcaat agataaaaca
     1861 ttgttccgca aaattatatt tgcagcggct gcaatgagca aacggcatat aaaaatatta
     1921 caccaat
//
LOCUS       DAOMAA010000412         1921 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_61326_length_1921_cov_1.950161, whole genome shotgun sequence.
ACCESSION   DAOMAA010000412 DAOMAA010000000
VERSION     DAOMAA010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61326_length_1921_cov_1.950161"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..875
                     /locus_tag="PLO59_06170"
     CDS             <1..875
                     /locus_tag="PLO59_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002673242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HQV00726.1"
                     /translation="GIGPTYMDKTGRNGLRVGDIEASDFKNRYKALTDKHKQMLGFYN
                     HAYDIEQLEAAWFEGVELLKTFTLIDSEYSMDKYLNQGKKILAEGAQGSLLDIDFGSY
                     PFVTSSNTITAGTCTGLGIAPSRIGEVFGIFKAYCTRVGSGPFPTELNDETGENMRVK
                     GNEFGSTTGRPRRTGWLDLPALRYAIMLNGVTQLCMMKSDVLSGFDQLKVCTAYDVDG
                     QIIKHIPFDVIHANMQPVYETLPGWQDDLTSITQAGQLPQALQHYIQFIENELKVPVK
                     IVSVGPNRLQTMWR"
     gene            948..>1921
                     /locus_tag="PLO59_06175"
     CDS             948..>1921
                     /locus_tag="PLO59_06175"
                     /inference="COORDINATES: protein motif:HMM:NF024500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HQV00727.1"
                     /translation="MHKLIYAQVNPAQITIEQADALIGERLKGKDVKKLIGQVILGHE
                     GVLMYCDSAIFFDASNSADCFGNVRIVKGDSLQMRGDFLRYNGNTRQAQLIKNVMLTD
                     GKMVLNTDSLDYNRNTEVAYYQHNAKITDADNNLISKRGYYFLKEKTLYFKDKVVLSN
                     PKYFLYTDTLKYNTAVKIAYFMGPTRIHARTADSTVMYCETGWYQTLNDKSYFGKKAF
                     IQSKSQCMLADSILYDKRNGIGQAFNNVIIADTGQGVILNGSKGFMHEKKNWSYLTGR
                     ATLTQAIDNDSLYLHADTLFATYDTINKTKLYQAYHGVRIFKTDLQGR"
BASE COUNT          628 a          359 c          404 g          530 t
ORIGIN      
        1 aaggcattgg tccaacttat atggacaaga ccggacgtaa cggtttacgg gttggcgata
       61 ttgaggcaag tgattttaaa aaccgatata aagcacttac cgataaacac aagcaaatgc
      121 ttggttttta taaccatgct tatgatattg agcaattgga agcagcatgg tttgaaggtg
      181 ttgaattatt aaaaaccttt acgcttatcg acagcgaata cagtatggat aaatacctta
      241 atcaagggaa aaaaatattg gccgaaggtg cacaaggctc acttttagat attgattttg
      301 gttcataccc ttttgttaca tccagcaaca cgataactgc aggtacttgc accggcttag
      361 gtattgcccc atcgcgcatt ggcgaagtat ttggcatttt taaagcttat tgtacgcgtg
      421 taggaagcgg accgtttcca actgaattaa atgatgaaac gggcgaaaat atgcgcgtta
      481 aaggaaatga atttggcagc actaccggcc ggccacgcag aacgggctgg ttagatttgc
      541 cggcattacg ctatgcaata atgcttaacg gtgttaccca attatgcatg atgaaaagcg
      601 atgtgctctc aggcttcgat caactaaaag tatgtactgc ttacgatgtt gacgggcaaa
      661 ttataaaaca cattccattc gatgtgatac atgccaatat gcagccggtt tatgaaacat
      721 tgcccggctg gcaggatgat ttaacatcca taacccaagc aggtcaactg ccacaagctt
      781 tgcagcatta catccagttt attgaaaacg agttaaaggt gccggttaaa attgtttcag
      841 taggccctaa ccgcttgcaa accatgtggc gataagttgg aatgaggcca atagttataa
      901 caacaattaa gcataagttt ttaacttgtt ggttggtgct aatttttatg cacaaactta
      961 tttatgctca agttaatccg gcacaaatta caattgaaca agccgatgca cttataggcg
     1021 aacgcctaaa gggtaaggat gttaaaaagc tgataggcca ggtaatatta ggtcacgaag
     1081 gggtattaat gtattgcgac agcgccatat tttttgatgc atccaacagt gccgattgct
     1141 ttggtaatgt gcgcatagtt aaaggggaca gcttacaaat gcggggcgat tttttaaggt
     1201 acaatggcaa taccagacaa gcacagctaa taaaaaacgt tatgcttacc gatggtaaga
     1261 tggttttaaa taccgactcg cttgattata acagaaacac agaagtagca tactatcaac
     1321 acaatgctaa aattaccgat gccgataata atttaatcag caagcgcgga tattactttt
     1381 taaaagagaa aacactttac tttaaagata aggttgtgct aagcaacccc aaatattttc
     1441 tttataccga tactttaaaa tataacacgg cagttaaaat tgcttatttc atgggcccta
     1501 cacgcataca tgcccgcaca gccgatagta cagttatgta ttgcgaaaca gggtggtacc
     1561 agacattgaa cgacaaatca tactttggta aaaaagcctt tatccaaagt aagtcgcaat
     1621 gtatgttggc cgatagtatt ttatatgaca aacgaaatgg aatagggcag gcttttaata
     1681 acgtgattat tgccgatacc ggtcaaggtg ttattttaaa cggaagcaaa ggctttatgc
     1741 atgaaaagaa gaattggtct tatctaaccg gccgcgccac attaacgcaa gcaattgata
     1801 acgacagttt atatttacat gccgatacac tttttgctac atacgataca attaataaaa
     1861 ctaaactgta ccaggcttac catggtgtgc gcatttttaa aaccgattta caaggccgat
     1921 g
//
LOCUS       DAOMAA010000413         1914 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_61732_length_1914_cov_2.268962, whole genome shotgun sequence.
ACCESSION   DAOMAA010000413 DAOMAA010000000
VERSION     DAOMAA010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61732_length_1914_cov_2.268962"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(212..>1914)
                     /locus_tag="PLO59_06180"
     CDS             complement(212..>1914)
                     /locus_tag="PLO59_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016197087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HQV00728.1"
                     /translation="GELIISSGQEITEDYADVIAESPIESVEIRSVLTCESKQGVCAR
                     CYGRNLATCRIVSRGEAVGVIAAQSIGEPGTQLTLRTFHAGGAARNLAQESRIEAKFA
                     GKVEFDEVRTIPYKTEEKLIDVVIGRSGEMRIVDTKTGAILATNNIPYGSHLSIKDGD
                     MVKKGDLICEWDPYNAVIVTEATGKIDYDNIDEGVTYREEQDEQTGYKEKVIIDSRDK
                     TRNPAIKILGSDKAALKSYNIPVGAHIIVNKGDKITAGQIIAKLPRSSAKAGDITGGL
                     PRVTELFEARNPSNPAVVSEIDGMVAYGGIKRGNREIIVTAKDGEVKKYLVPLSKHIL
                     VQDNDYIRAGHPLSDGATSPGDILAIKGPGAVQEYLVNEVQEVYRLQGVKINDKHFEV
                     IVRQMMRKVQIEDPGDTRFLEKELVNRLDFIEENDAIFDKMVIMEEGDSTQFKAGQIV
                     SVRKLRDENSTLKRAGKTPVEARQAVPATSTPVLQGITQASLQTKSWISAASFQETTK
                     VLNEAAINGKVDHLLGLKENVIVGHLIPAGTGLREYQQYIVGSKAEYEALMATQEDEA
                     "
BASE COUNT          498 a          412 c          345 g          659 t
ORIGIN      
        1 gttgatttag gttttgtttc tgcgctcaat ggaaatgtgt ttttgtcaaa aataaacgct
       61 aacaactata tataaaaata tgctgtttaa ataatttata aatcgtttaa tatttcgagg
      121 ttttagaaaa attatgcttg tgtatgaagc taaaaaaggc tgcctattta taagacagcc
      181 tttagttgaa attgattact taaattaaat attaagcttc gtcttcttgt gttgccataa
      241 gtgcttcgta ctcggcttta ctaccaacta tgtattgttg atattcgcgt aaacctgtac
      301 cggccggaat taaatggcca acaattacgt tttctttcaa tcctaataag tgatccactt
      361 ttccattgat agcggcttcg ttaagaactt ttgttgtttc ctggaacgat gcagccgaaa
      421 tccaactctt agtttgtaaa gatgcctggg taataccttg taataccgga gtagaggttg
      481 caggcactgc ctgacgtgct tctaccggag ttttacctgc gcgttttaag gttgagtttt
      541 cgtcacgcaa tttacgtacg cttactattt gacctgcttt aaactgggtg ctgtcgcctt
      601 cttccataat aaccatttta tcaaaaatgg catcattttc ttcaataaag tctaaacggt
      661 ttaccaattc tttttcaagg aagcgtgtat cgcccggatc ttcgatttgt accttacgca
      721 tcatttgacg cacaattact tcgaagtgtt tgtcattaat ctttacacct tgtaaacgat
      781 aaacctcctg tacttcattt accaaatatt cttgtacggc tcccggtcct ttaatcgcta
      841 atatatcacc gggtgatgta gcaccatcac ttaacggatg acctgcacgt atgtagtcat
      901 tatcctgaac caatatatgc ttgctaagtg gaactaagta tttttttact tcaccatctt
      961 ttgcagttac aataatttcg cggttaccgc gcttaatacc accataagct accataccgt
     1021 caatttcgct aactaccgct gggttacttg ggttacgtgc ttcgaataat tcggttacgc
     1081 gtggtaatcc tccggtaata tcaccggctt tagccgaaga gcgaggtagc tttgctatta
     1141 tctgacctgc cgtaattttg tcgcccttgt ttacaataat gtgtgcgcca acaggtatgt
     1201 tgtatgattt tagtgctgct ttatcgctgc ctaaaatttt aatagctggg ttgcgtgttt
     1261 tatcgcgact atcaataatt actttttcct tatatccggt ttgttcatct tgttcttcgc
     1321 gataggttac accttcatca atattatcgt aatcaatttt accggttgct tcggttacaa
     1381 ttactgcatt gtaaggatcc cattcacaaa tcaaatcgcc ctttttaacc atatcgccat
     1441 ctttaattga aagatgcgaa ccatagggta tattgttggt agctagtata gctccggttt
     1501 ttgtatctac aatacgcatt tcgccactac gaccaataac tacgtcaatt aatttttctt
     1561 cggttttata aggtatggta cgtacttcat caaattcaac cttaccggca aattttgctt
     1621 cgatacggct ttcttgcgct aagttacgcg ctgcaccacc ggcatggaat gtacgaagtg
     1681 ttaactgtgt accaggttcg ccaattgatt gtgctgcaat aacaccaaca gcttcgccac
     1741 gcgacacaat acgacacgta gctaagttgc gtccataaca acgtgcacaa acaccttgtt
     1801 tactttcgca agttaacacc gatcggattt caaccgattc gattgggctt tcggcaatta
     1861 catcggcata gtcttcggta atttcctgtc cgctgcttat aattaattcg cctg
//
LOCUS       DAOMAA010000414         1912 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_61829_length_1912_cov_2.983306, whole genome shotgun sequence.
ACCESSION   DAOMAA010000414 DAOMAA010000000
VERSION     DAOMAA010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61829_length_1912_cov_2.983306"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1741)
                     /locus_tag="PLO59_06185"
     CDS             complement(<1..1741)
                     /locus_tag="PLO59_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HQV00729.1"
                     /translation="MEYAFKQVESKWQQYWKQQGAYTSNDFNKPKFYVLDMFPYPSGA
                     GLHVGHPLGYIASDIYARYKRLQGFNVLHPMGYDSFGLPAEQYAIQTGQHPAITTQQN
                     IARYRQQLDMLGFSYDWQREIRTSEPDYYKWTQWIFIQLFHAWYDNTLQKAMPIESLV
                     QQFETNGNSGINAATEYENTFTAAQWLAYNQAQQHEILMAYRLAYLSNTLVNWCAALG
                     SVLANDEVKDGVSERGSYPVERKLMQQWSLRITAYSQRLLDDLETIDWTESIKDSQRN
                     WIGRSQGTSINFNCNNQTITVFTTRPDTVFGVTYLTLAPEHPLVSQLTLATHKSEVDQ
                     YVAWAKNRSERERQADVKHISGVFTGAYAIHPFTNEPIAVWIGDYVLAGYGTGAVMAV
                     PAHDTRDYAFARHFNLPVKQVIEPTTPHNFNEAAYDEKMGTCINSGFLNGLSVNEAIK
                     VAISKIENEAVGIGQVNYRQRDAVFGRQRYWGEPIPVYYKDGIAHTLPTDALPLVLPA
                     IDAYLPTEQGEPPLARAQNFTYNGYPIETTTMPGWAGSSWYYLRYMDAGNTQAFASAD
                     AINYWQQIDLYLGG"
BASE COUNT          508 a          407 c          423 g          574 t
ORIGIN      
        1 tgccgcctaa atacaaatca atttgttgcc agtaattaat ggcatcggcc gatgcaaatg
       61 cttgtgtgtt gccggcatcc atataacgca aatagtacca actgctaccg gcccaaccgg
      121 gcatggttgt agtttcgatg gggtagccat tgtaggtaaa gttttgtgca cgggccaaag
      181 gtggttcgcc ttgttcggtt ggcaggtaag cgtcaattgc cggtagcacc agtggcaatg
      241 catcggttgg taacgtatgt gctatgccat ctttataata aaccggtatg ggctcgcccc
      301 aataacgctg gcggccaaat accgcatctc gctggcggta atttacctga ccaataccca
      361 ctgcttcatt ttcaatttta ctgattgcaa ctttaatggc ttcgttaacg cttaatccgt
      421 ttaagaaacc actgttaata caagtgccca ttttttcgtc ataagcagct tcattaaaat
      481 tgtgtggcgt ggtgggctca ataacttgtt taacgggtaa gttaaagtgg cgggcaaaag
      541 catagtcgcg ggtatcgtgt gccggtacag ccattacagc cccggtgcca taaccggcaa
      601 gcacataatc gccaatccaa accgctatag gttcattagt aaaaggatgt atggcataag
      661 cgcctgtaaa gacaccagaa atgtgtttaa catcggcctg gcgctcgcgc tcgctacggt
      721 ttttggccca ggcaacgtac tgatctactt cggatttatg tgtagccaat gttaattggc
      781 taaccaacgg atgttcgggt gccagggtaa gataggtaac accaaaaacg gtatcaggcc
      841 tggtagtaaa aacagtgata gtttggttgt tgcaattgaa atttatggaa gtgccttggc
      901 tgcggccaat ccaattgcgt tgcgagtctt taatactttc cgtccagtca attgtttcta
      961 aatcgtctaa caagcgctgc gaataagcgg ttatgcgtaa actccattgc tgcattaatt
     1021 tgcgttcgac cggatagcta cctctttcgc ttacaccatc tttaacctca tcgttagcta
     1081 aaacactacc taaagcggca caccagttaa ccagtgtatt gcttaaataa gccaaacggt
     1141 aagccattaa tatttcatgc tgctgtgcct gattgtaagc aagccattgt gcggctgtaa
     1201 aagtattttc gtattcagta gctgcattaa taccgctatt accattggtt tcgaactgct
     1261 gcaccaaact ttcaataggc attgcttttt gtaaagtatt atcataccag gcatgaaaca
     1321 gctgaataaa aatccattgt gtccatttat aataatcggg ttcgctggtg cgtatttcgc
     1381 gttgccagtc gtaactaaag cccagcatat ctaattgctg cctgtagcgg gcaatatttt
     1441 gttgtgtggt tattgccgga tgctggcctg tttgtatggc atattgttcg gcaggtaagc
     1501 caaacgaatc gtagcccatg gggtgtaaaa cattaaaccc ttgcagtcgt ttgtaccgtg
     1561 catatatatc acttgcaata taacctaagg gatggcctac atgtaaaccg gcaccgctgg
     1621 ggtatggaaa catatctaac acataaaact taggcttatt aaaatcgttc gaagtatagg
     1681 cgccttgttg tttccaatac tgttgccact tactttctac ttgcttaaag gcgtattcca
     1741 taattacatt ttataaaaac agggttgcga atgtaaatat ttgccgttta ccttatggct
     1801 tattggctgt tggggttgtt tacgctaacc tttcagattt gcacatttat gtattcataa
     1861 tttattggtt attacacatg gagtaaataa tccggagcgt ttacaaactg ta
//
LOCUS       DAOMAA010000415         1911 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_61904_length_1911_cov_2.574353, whole genome shotgun sequence.
ACCESSION   DAOMAA010000415 DAOMAA010000000
VERSION     DAOMAA010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61904_length_1911_cov_2.574353"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="PLO59_06190"
     CDS             <1..487
                     /locus_tag="PLO59_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HQV00730.1"
                     /translation="IKNMKLKIVEPLKTLFKDEVRLIGKALGLTAVLLDRHPFPGPGL
                     GIRILGDITPDKVEILQQVDSIFINGLRKHNLYDKTWQAGCILLPVQSVGVMGDERTY
                     EKCVALRAVTSTDGMTADWAHLPYDFLAGISNEIINNVKGVNRVVYDISSKPPATIEW
                     E"
     gene            529..1302
                     /locus_tag="PLO59_06195"
     CDS             529..1302
                     /locus_tag="PLO59_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HQV00731.1"
                     /translation="MTHGICNQSIVPMRADAAHKSEMVSQLLFGELFEIIQVQNEWLR
                     IKTDYDQYNGWVLATQVQMLELMAFKSIKKQPLFLCNDLIGVLDGGMPFPVVLGSTLP
                     NYDYKKCSVLDQHYHFHGEAISTPHKTISIANFVEHAMLYLNAPYLWGGRSPLGIDCS
                     GFTQMVFKLAGIKLKRDAYLQAEEGNQILLLNETQPADLAFFENDEGRITHVGLLIGE
                     NKIIHASGKVRVDTIDHFGIYNDELKKYSHKLRIIKRMI"
     gene            1302..>1911
                     /locus_tag="PLO59_06200"
     CDS             1302..>1911
                     /locus_tag="PLO59_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00732.1"
                     /translation="MKFLLYAIASILITGCAKPVSPAETNNKFSGTWVNQSYIDTVAH
                     YTKADLFRTIKLPFALQIETLNDSVLMLHNGFETYRVNYRYQTDTLQIPTMQITMLYD
                     SVSNSLSDNKNNHWKKVTPTENTEAYIQSLNKILLAGNYKLLNNSTDDEIQFEASGKI
                     NGWLGATIYRLCIAGDCLHEFAFKGNTMVMHFKNDDTRYYGWQ"
BASE COUNT          632 a          346 c          365 g          568 t
ORIGIN      
        1 aataaaaaac atgaaactaa aaattgttga accgcttaaa acacttttta aagatgaggt
       61 gcgtttaata ggcaaagcat tgggacttac ggccgtattg ttagacaggc acccttttcc
      121 gggccccggt ttgggcatca gaatcttagg cgacataaca cctgacaaag ttgagatact
      181 acaacaggtt gacagtattt ttattaacgg cttgcgcaaa cataatttat acgataaaac
      241 ctggcaggct ggttgcattt tattgccggt acaaagtgtg ggcgttatgg gcgatgaacg
      301 tacatatgaa aaatgtgtgg cactgcgtgc cgtaacttct accgatggta tgacagccga
      361 ttgggcccac ctaccctacg attttttggc cggcatcagc aatgaaatta ttaataatgt
      421 aaaaggtgtt aaccgggttg tatatgatat ttcatcaaaa ccaccggcaa ccatcgaatg
      481 ggagtaaaac aacctatata acaacagtta caatttacat taacttttat gacacacggc
      541 atttgtaacc aaagtattgt acccatgcgg gccgatgccg cacataaaag tgagatggta
      601 tcgcaattac tttttggcga actgttcgaa attattcaag ttcaaaacga atggttgcgt
      661 ataaaaaccg attacgacca atacaacggt tgggtgttgg caacacaagt tcaaatgctt
      721 gaattaatgg cttttaaatc cattaaaaag caacctcttt ttttatgcaa tgatttaatt
      781 ggcgttttag atggcggcat gccttttcct gttgttttag gtagtacatt acccaattat
      841 gattataaaa aatgtagtgt tttagatcaa cattatcatt ttcatggcga agcaataagc
      901 actccgcata aaactataag tattgccaac tttgtcgaac atgccatgct ttatttaaat
      961 gcaccgtatt tatggggtgg caggtcgcct ttaggtattg attgctcggg ctttactcaa
     1021 atggttttta agttagcagg cattaaacta aaacgcgatg cctatttgca agccgaagaa
     1081 ggcaatcaga tacttttact taacgaaacc caacctgccg acctggcttt tttcgaaaat
     1141 gatgaaggtc gcattaccca tgtgggtttg ttaattggtg aaaataaaat tatccatgca
     1201 tcgggtaaag tacgtgttga taccattgat cattttggca tatacaatga tgagctaaaa
     1261 aaatattcac ataaacttcg catcataaaa agaatgattt aatgaagttt ttactttatg
     1321 ctattgcaag tattttaata accggttgtg caaagccggt atcgcctgct gaaactaaca
     1381 ataaattcag tggtacttgg gtaaatcaaa gttatattga taccgttgca cattatacta
     1441 aggccgattt gtttcgaacc attaaactac cctttgcctt acaaatagaa accctgaatg
     1501 acagtgtatt aatgttacat aatggtttcg aaacctatcg ggtaaactac cgctatcaaa
     1561 ccgatacatt acaaatacct acaatgcaaa ttacaatgtt gtacgactcg gtaagcaaca
     1621 gtttatctga taataaaaac aaccattgga aaaaagtaac gcctacagaa aatacagaag
     1681 catacataca atcattaaat aaaattttac ttgcaggtaa ctacaaattg ctcaataaca
     1741 gtacggatga tgaaatacaa tttgaagcat ccggaaaaat aaatggctgg cttggtgcta
     1801 ctatttacag attgtgtatt gctggcgatt gcttacatga atttgcgttt aaaggcaata
     1861 ccatggtaat gcactttaaa aatgatgata cccgttatta tggctggcaa t
//
LOCUS       DAOMAA010000416         1902 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_62480_length_1902_cov_2.861938, whole genome shotgun sequence.
ACCESSION   DAOMAA010000416 DAOMAA010000000
VERSION     DAOMAA010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62480_length_1902_cov_2.861938"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..322
                     /locus_tag="PLO59_06205"
     CDS             119..322
                     /locus_tag="PLO59_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020533406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HQV00733.1"
                     /translation="MYILKIKGAAKIPDYVQLRDAHFTLIAYFRVDRPEKALIKCGFA
                     DREAELIKIINEMPFGKVQALEF"
     gene            368..1045
                     /locus_tag="PLO59_06210"
     CDS             368..1045
                     /locus_tag="PLO59_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015361070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQV00734.1"
                     /translation="MLTEDMVINLNNVSVYQDKNLILNNIVLQINKGEFVYLIGKTGS
                     GKSSLLKLLYADLNLKHGQGAVADYDLRKLKRKDIPHLRRKIGIVFQDFQLMGDRSVR
                     DNLLFVMKATGWDDKHKMEARIRDVLSKVNLGTKGFKMPHELSGGEQQRLVIARALIN
                     EPELIIADEPTGNLDPETSDEIMKLLFDIAQSGRSVLMATHNYALIERYPSRVIKCER
                     GNLFNHA"
     gene            complement(1036..>1902)
                     /locus_tag="PLO59_06215"
     CDS             complement(1036..>1902)
                     /locus_tag="PLO59_06215"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQV00735.1"
                     /translation="QNLLNDNYPILFEGLHCCFYLDDARLKHRVRIVRTHNIEHHYYQ
                     SLARIETNIFKRYYFYNEAEKLATFEQNLRHATYIAAISPNDNMYFNKQYEHAQYVPA
                     FHAHTQMLFKPGKGSYALYHGNLSIGENNEAALYLINEVFSKTTYHLVIAGSKPSKAL
                     RAAIDKHQNISLRADISVAQIETLIAEAHINVLPTFQPTGIKLKLLSALYLGRHCLVN
                     DVMVSNTGLEPLCYVANNAQEFIVKLKQLFKKSFTQADCQKRTEILLQKFSNETGAQK
                     LVAMINSYVYQA"
BASE COUNT          617 a          328 c          358 g          599 t
ORIGIN      
        1 ttaagtcaaa cggcatttta ttttcctaac aaaacattgt acatatttaa atagaaacaa
       61 ccattttttg ccatgcgtat taaactatgt ttgtatgcac aagttgctgt ttcaaatgat
      121 ttatatttta aaaataaaag gcgctgcaaa aattccagat tatgtacaac tgcgtgatgc
      181 acatttcaca ttaattgcct acttcagagt tgaccgacca gagaaagcac ttataaaatg
      241 tggctttgcc gaccgcgaag cagagcttat taaaattata aatgaaatgc cttttggcaa
      301 agtacaggct ttggaatttt aatctcccaa aatttaactg ttactataca tacatttata
      361 caacaggatg ctaacagaag atatggtaat taacttaaac aatgtttcag tatatcagga
      421 taaaaatctg atacttaata atattgtatt gcaaattaat aaaggtgagt ttgtttatct
      481 tataggcaaa acaggtagtg gcaaaagcag tctgttaaaa ttgctttatg ccgatttaaa
      541 tttaaaacac ggacaaggtg ccgttgccga ttatgatttg cgaaaactaa aacgaaaaga
      601 cataccgcac ctgcgcagaa aaattggcat tgtgtttcag gattttcaac tgatgggcga
      661 cagaagtgtg cgcgacaact tattgtttgt gatgaaagca accggctggg acgacaaaca
      721 caaaatggaa gcccgcatac gtgatgtttt aagtaaagta aatttaggga caaagggctt
      781 taaaatgccg catgaactaa gtggtggcga acaacaacgt ttggtaatag cccgtgcatt
      841 aataaacgaa cccgagctaa ttattgccga tgaacccaca ggcaatctcg accccgaaac
      901 ttcggatgag attatgaaac tactttttga tattgcacaa agcggccgca gtgttttaat
      961 ggccactcat aattatgcat taatagaacg atacccatcg cgggttatta agtgcgagcg
     1021 tggtaatttg tttaatcatg cctgatatac atagctatta atcatggcca ctaacttttg
     1081 agcaccggtt tcatttgaaa atttttgcaa taaaatttca gtgcgttttt ggcaatcggc
     1141 ttgggtaaac gattttttaa ataattgttt cagttttaca ataaattcct gcgcattatt
     1201 agccacataa caaagtggct ctaagcccgt gttcgatacc attacatcat ttaccaaaca
     1261 atgcctgcct aaatacaatg ccgagagcaa ctttaacttt atacctgtag gctgaaaagt
     1321 gggcaataca tttatatggg cttcggcaat tagtgtttca atttgtgcca cacttatatc
     1381 agctctcagg cttatgtttt gatgtttgtc aatggcagca cgcaatgctt ttgatggttt
     1441 gcttccggca ataactaaat gataggtagt tttcgaaaac acttcattta taagatataa
     1501 agctgcttcg ttgttttcgc caatacttaa attaccatgg tataaagcat agctgccttt
     1561 accaggctta aataacattt gtgtatgtgc atgaaaagca ggtacatatt gtgcatgttc
     1621 atattgctta ttaaaataca tattatcgtt gggtgatata gcggcaatgt atgtggcatg
     1681 ccttaagttt tgctcaaatg ttgccagctt ttcggcttcg ttgtaaaaat agtaacgttt
     1741 aaaaatattg gtttcgatac gggccaaact ttgataataa tgatgttcga tattatgtgt
     1801 tcgtacaatt cgaacgcggt gttttaaacg ggcatcatct aaataaaaac aacaatgtaa
     1861 gccttcgaac aatatcggat aattatcatt taacaggttt tg
//
LOCUS       DAOMAA010000417         1897 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_62732_length_1897_cov_3.833876, whole genome shotgun sequence.
ACCESSION   DAOMAA010000417 DAOMAA010000000
VERSION     DAOMAA010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62732_length_1897_cov_3.833876"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1433
                     /locus_tag="PLO59_06220"
     CDS             <1..1433
                     /locus_tag="PLO59_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQV00736.1"
                     /translation="AGNNVTGVRSRYIVEDRITKINKLNFNTYYNNIINDKLTLTAGA
                     THQRQNSAFYKEVNDLLGGEFYVDYNQFAEQDYPGNTDALQNDLNNPNRILKVGDKFG
                     YHYVAHVNRTNAWIQPQLKLNKVDLFIALDVSHTAFYRDGKVRNGIFSENSYGKSDKQ
                     SFTNAAIKGGATYKINGRNYVFANVATLTRAPLFENAFVSARTRNSIIDNLTDEKITS
                     VEGGYMLKAPKLKARVVGYYTQFNDGVNVMSFYHDDFRTFVNYSLTNIDKVNTGLELA
                     VDANLGKGFSAVAVASIGQNRYTSRQFGTLTQDNSDTVLASETIYIKNFKVGGTQAAY
                     MLGISYRSPKFWFLSLNVNYFDKIFVEANPARRTQAAVDLVDPQSENWDKIINQQQLD
                     GQLTVDFFGGYSYKLNNKFKNLKRQSFIVLNIGVNNLLNNTDFRTSGFEQLRYDFNDK
                     NPEKFPAKYFYSFGTTFFANLTLRFN"
     gene            1511..>1897
                     /locus_tag="PLO59_06225"
     CDS             1511..>1897
                     /locus_tag="PLO59_06225"
                     /inference="COORDINATES: protein motif:HMM:NF040146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5689 domain-containing protein"
                     /protein_id="HQV00737.1"
                     /translation="MMSGVTSCVKKEYDEPPTDGTNPNITVTHTIAQIKAMYAGTPLR
                     IDSDYVISGVVIADDKSGNFYKSVVIQDSTAGIAIRLDQSDFYTDFPAGRLLFIKCKG
                     LYIGDYGGLIQMGGYVDNSGSTPSVEP"
BASE COUNT          627 a          369 c          348 g          553 t
ORIGIN      
        1 ttgccggaaa taacgttacc ggtgtacgtt ccaggtatat tgtagaagat cgcatcacca
       61 aaataaataa acttaatttc aatacatact ataacaacat tataaacgac aagctaacac
      121 ttactgccgg tgctacacat caaaggcaaa acagtgcttt ttacaaggaa gttaacgatt
      181 tattgggagg cgaattttat gttgattaca atcagtttgc cgagcaagat tatccgggca
      241 acaccgatgc attacaaaac gatttaaata accccaaccg cattttaaaa gtaggcgata
      301 agtttggcta ccattatgta gcacatgtaa atcgaaccaa tgcatggata caaccccagt
      361 taaaacttaa taaggttgat ttatttattg cattagatgt ttcgcataca gcattttatc
      421 gcgatggcaa agtacgtaac ggtattttta gcgaaaactc ttatggcaaa tccgataaac
      481 aaagctttac caatgcagcc attaaaggcg gtgctactta taaaattaat gggcgtaatt
      541 atgtatttgc caatgtagcc acgcttacac gtgcacccct tttcgaaaac gcatttgtat
      601 cggcacgcac acgcaacagt attattgaca acttaacgga cgaaaaaata acttcggttg
      661 aaggtggtta tatgcttaaa gcgcccaaac ttaaagcacg cgttgttggt tactacacgc
      721 aatttaacga tggcgttaat gtgatgagtt tttatcatga cgatttccga acgtttgtaa
      781 actactcgtt aaccaatatt gataaagtta acaccggttt agaattggca gttgatgcaa
      841 atttaggcaa aggttttagc gctgttgctg tagcttctat aggccaaaac cgttatacat
      901 cgcggcagtt tggaacgctt acacaagaca acagcgacac cgtacttgcc agcgaaacta
      961 tttacattaa aaattttaaa gttggtggta cacaagccgc ttatatgttg ggcatatcat
     1021 atcgctcacc taaattttgg ttcttaagtt taaatgtaaa ttatttcgat aaaatttttg
     1081 tagaagccaa cccggctcgc agaacacaag ctgctgttga tttagtggac ccacaaagcg
     1141 aaaactggga caaaattatt aaccaacaac aacttgacgg acaactaaca gttgatttct
     1201 ttggtggcta ttcttacaag ctaaataata aatttaaaaa cttaaaacgt caatcgttta
     1261 ttgttctaaa cattggtgta aacaacttgc taaacaacac cgatttcaga accagtggtt
     1321 tcgagcaatt acgctacgac tttaatgata aaaaccccga aaaatttccg gcaaaatatt
     1381 tttacagttt cggaacaacc ttttttgcca acctcacttt acgtttcaat taatataaac
     1441 atttgcttag tataaaatca cattgcatga aaagctttat taaatcatta accggtttgc
     1501 tattatttta atgatgtcgg gtgttacatc ctgtgtaaaa aaagaatatg acgaaccacc
     1561 aaccgatggt accaacccca acattacagt tacgcatacc attgcccaaa taaaagcaat
     1621 gtatgccggc acacctttgc gtatcgactc cgattatgtt ataagtggtg tggtaatagc
     1681 cgatgataaa tcgggcaatt tttataaatc tgtagtcatt caagactcaa cagccggtat
     1741 cgctatcagg ttagatcaga gtgattttta caccgatttt ccggcaggca ggttgttatt
     1801 catcaaatgt aaaggcctat atattggcga ttatggcggc ctgattcaaa tgggaggtta
     1861 tgtagataac agcggcagta cacccagtgt tgaaccg
//
LOCUS       DAOMAA010000418         1896 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_62828_length_1896_cov_2.862575, whole genome shotgun sequence.
ACCESSION   DAOMAA010000418 DAOMAA010000000
VERSION     DAOMAA010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62828_length_1896_cov_2.862575"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1162
                     /locus_tag="PLO59_06230"
     CDS             <1..1162
                     /locus_tag="PLO59_06230"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HQV00738.1"
                     /translation="FGRIPIIAITGTNGKTTTATLLYQLFKDLGYKTGLLSTVKNKIN
                     DTEIEATHTTPDALTLHKLLQQMVEAGCTHAFMEVSSHAVVQQRIAGVQFAGAVFTNI
                     THDHLDYHKTFDAYIAAKKGFFDGLPDTAFALTNDDDKNGKVMLQNTKAKKYTYALRT
                     HADFKCKVLENNFGGLMLQVNKHEVLCKLVGTFNAYNIVSVYGTAVLLGEEPISVLKV
                     LSNLQPPAGRFEYVVSNQITGIVDYAHTPDAVKNVLATIGDIRTGNEKVITVIGCGGD
                     RDKTKRPEMAKLASMLSNKVIITSDNPRSENPLTIIAEMQAGVPASKYKNVLAIADRK
                     EAIRTACNLAAKGDIILVAGKGHEKYQEIQGVKYPFDDLEILTQTLNQNQIQ"
     gene            1174..>1896
                     /locus_tag="PLO59_06235"
     CDS             1174..>1896
                     /locus_tag="PLO59_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006259687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HQV00739.1"
                     /translation="MLYYLFELLDKQYDIPGAGVFKYISFRTAMSLIVSLLFSLFIGK
                     RIIKMLYQKQVGETIRDLGLAGEKEKKGTPTMGGLIILSSILLPTFLFARLDNVYIVT
                     LLISTVWLGIIGFLDDYIKVFKKDKEGLAGRFKIMGQVGLGVIVGCILSFNEYVVVRE
                     KIPVSDFNVSAKGSVIENISNTSGVLYSTQNVKSTKTTIPFLKNHEFDYTSLVSWLGD
                     DTKKYAWIAFTLVVIFIVTAVSN"
BASE COUNT          633 a          323 c          383 g          557 t
ORIGIN      
        1 ttttggtcgt attccaatta ttgcaattac aggtaccaat ggtaaaacaa caactgcaac
       61 attgctgtac cagttgttta aagatttagg ttacaaaaca ggtttgctgt caaccgttaa
      121 aaacaaaatt aacgataccg aaattgaagc aacacatacc acacctgatg cattaacact
      181 tcataaatta ttacaacaaa tggtagaggc aggttgtaca catgcattta tggaagttag
      241 ctcgcatgca gtggtgcagc aacgtattgc cggtgtgcaa tttgccggtg cagtgtttac
      301 caacatcacc cacgaccatc ttgattacca taaaacattt gatgcataca tagcagctaa
      361 gaaaggtttt tttgatggct tacccgatac tgcatttgcg cttacaaatg acgatgataa
      421 aaatggtaag gtaatgctgc aaaacaccaa ggcaaaaaag tacacttatg ccttacgcac
      481 acatgccgat tttaaatgca aagtacttga aaataatttt ggcggtttaa tgcttcaggt
      541 aaacaagcat gaagtgcttt gtaaactggt aggtactttt aatgcctata atattgtatc
      601 agtttatggt actgcagttt tattaggcga agagcccatt tcagttttaa aggtacttag
      661 caatttgcag ccacctgcag gtaggtttga gtatgtggtt agtaatcaaa ttacaggcat
      721 agttgattat gcacatacac ccgatgcggt aaaaaatgta cttgctacca taggtgatat
      781 acgcacaggt aacgaaaaag taataaccgt aattggttgt ggcggtgata gggacaaaac
      841 caaaaggccc gaaatggcaa agctggcatc aatgcttagc aataaggtaa ttattaccag
      901 cgataatccg cgcagcgaaa acccgttaac catcattgcc gaaatgcagg caggtgttcc
      961 ggcttcaaag tacaaaaacg ttttggccat tgccgataga aaggaagcaa tacgcaccgc
     1021 ttgcaattta gctgcaaaag gcgatatcat tttggttgct ggcaagggcc atgaaaaata
     1081 tcaggagata caaggagtaa aatatccgtt tgacgacctc gaaattttaa ctcaaacact
     1141 caatcaaaac caaatacagt aaggtacata acaatgttat attacttatt cgaactatta
     1201 gataaacaat atgatatacc cggtgcaggt gtgtttaaat acatatcgtt tcgcacggct
     1261 atgtcattaa tagtttcgtt gttgttttcg ctttttatcg gtaaacgcat tattaaaatg
     1321 ctttatcaaa aacaagttgg cgaaaccatt cgcgatttag gtttggcagg cgaaaaggaa
     1381 aaaaagggta caccaacaat gggtggcctt atcattttat cttcaatttt attgcctacc
     1441 tttttatttg ctcgtttgga taacgtgtat atcgtaaccc ttttaattag tacggtatgg
     1501 ttaggtataa ttggtttttt ggatgattac atcaaagtat ttaaaaaaga taaagaggga
     1561 ttagcgggcc gttttaaaat aatgggacag gtaggattag gtgtaatagt aggctgcata
     1621 cttagtttta atgaatatgt tgtggtacgc gaaaaaattc cggtaagcga ttttaatgta
     1681 agcgcaaagg gtagtgtaat tgaaaacata agcaatactt cgggtgtact ttattctaca
     1741 caaaatgtta agtcaacaaa aacaacgata ccatttttaa aaaatcacga gttcgattac
     1801 acttcattag taagctggct tggtgacgat actaaaaaat atgcatggat tgcgtttaca
     1861 ctggtagtta tatttattgt aaccgcagtt tcaaat
//
LOCUS       DAOMAA010000419         1890 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_63215_length_1890_cov_3.728610, whole genome shotgun sequence.
ACCESSION   DAOMAA010000419 DAOMAA010000000
VERSION     DAOMAA010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63215_length_1890_cov_3.728610"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..805
                     /locus_tag="PLO59_06240"
     CDS             116..805
                     /locus_tag="PLO59_06240"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV00740.1"
                     /translation="MAKRILIFLAASLLALQLPAQTVTTLKVTQFFVGGTAGFPDTIY
                     AGQAYTTSWVITNTGNAPFINAPANPLKILMQADSSFIPYELAATNSLFTLQPGDTVL
                     LTNVDSFPPGVFKLGNNVVVVWPSKNQPIDFIADSTYFYTYYVGLSGIDVTDGAGVLV
                     YPNPFSEMLFMQPLTDGDVSFQLINLTGQVVLAGNHLQQINTSFLRSGLYYLHIRQKQ
                     KATRTYKLLKY"
     gene            1042..>1890
                     /gene="ispG"
                     /locus_tag="PLO59_06245"
     CDS             1042..>1890
                     /gene="ispG"
                     /locus_tag="PLO59_06245"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HQV00741.1"
                     /translation="MESNLYCNSLTQYSRLKTRVVNVGEVPLGGNNPIRVQSMTTTDT
                     MHTEATVAQSIRMIEAGCEYVRITAPSINEAKNLENIKKELRLRGFKTPLVADIHFTP
                     NAAEYAARIVEKVRVNPGNYADKKKFETHDYTDASYNDELDRIRNRFIPLIKICKEYG
                     TAIRIGTNHGSLSDRIMSRYGDTALGMVESALEFLRICRDENFHQIVLSMKSSNTQVM
                     VQAYRLLIQKMHQTGMDYPLHLGVTEAGEGEDGRIKSAVGIGTLLEDGIGDTIRVSLT
                     EDPEFEI"
BASE COUNT          578 a          369 c          369 g          574 t
ORIGIN      
        1 ctaaaataca ttaatactcc aatgcttaat ttgactgtgt aagataaatt acaaataaag
       61 tacagtaatt atcagtttaa aactacttta gtaaaaattt ttagtacggt tatttatggc
      121 taagcgcatt ttgatttttc tagcagcaag tttactggct cttcaactgc ctgcacaaac
      181 cgttactaca cttaaagtaa cgcagttttt tgtgggtggc acagccggtt tccccgatac
      241 aatttatgcc ggccaggctt atacaacttc gtgggttata accaataccg gtaatgcccc
      301 ttttataaat gcacctgcca atcctttaaa aattcttatg caggccgaca gttcgtttat
      361 cccttatgaa cttgcagcaa ctaatagctt gtttacactg caaccgggcg atacagtttt
      421 acttacaaat gttgattcgt ttccgccagg tgtttttaaa ttaggtaaca atgtggtggt
      481 agtttggcca tctaaaaacc aacccattga ttttattgcc gacagtactt acttttatac
      541 gtattacgtt ggccttagcg gtattgatgt aaccgatggt gcaggtgtat tggtatatcc
      601 caatccgttt tcggaaatgc tctttatgca accattaacc gatggtgatg taagttttca
      661 gctaataaat ttaaccggtc aggtagtgct tgccggcaac catttgcaac aaataaatac
      721 aagcttttta cgtagtggtt tgtattattt acatatacgt caaaaacaaa aggcaacacg
      781 tacctataaa cttttaaagt actaactaca acgtttaagt ttgtaaccga tcgtttgaaa
      841 ttagtttcgg ctccgtattt ttagtttcgt tttttcgaat cgtacatttt tcattgcgca
      901 gcagttccct aatggttggc aatacgtagt accttatatt cggcaaaccg gctgcagttt
      961 ttattggcac attggtaaat taaatttact gaaaatactc gcctcatttt cgcttacctt
     1021 tgtttcgaat ttattaaaaa gatggaatca aatttatatt gtaacagtct tacgcaatat
     1081 tcgagattaa aaacaagggt ggtaaacgtt ggcgaggttc cactgggtgg caataaccca
     1141 ataagggtac aatctatgac cactaccgac accatgcata ccgaagcaac cgtggcacaa
     1201 agtatccgga tgatagaagc cggttgcgaa tatgtgcgta taaccgcacc cagcattaac
     1261 gaagcaaaaa acctggaaaa cattaaaaag gaattgcgct tgcgtggctt taaaacccca
     1321 ttagtagccg atattcattt tacccccaat gcagccgagt atgcagcacg tattgtagaa
     1381 aaagtaagag taaacccggg caactatgcc gacaaaaaaa aatttgaaac ccacgattat
     1441 accgatgctt cgtataacga tgaattagat agaattcgca atcgttttat acccttaatt
     1501 aaaatttgta aagaatatgg tacagccatt cgcattggca ccaatcacgg ctcgctaagc
     1561 gatcggatta tgagccgtta tggcgatacg gcattaggca tggttgaatc ggctttagag
     1621 tttttacgca tttgccgtga cgaaaatttt catcagattg tactttctat gaaaagcagt
     1681 aacacgcaag tgatggtgca ggcatacaga ttactgatac aaaaaatgca tcagacaggc
     1741 atggattatc cattgcattt aggtgttacc gaagcaggcg aaggcgaaga cggccgcatt
     1801 aaatcggctg tgggtattgg cactttatta gaagatggta ttggcgatac cattcgtgtt
     1861 tcgttaaccg aagatcccga atttgaaata
//
LOCUS       DAOMAA010000420         1890 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_63237_length_1890_cov_2.793460, whole genome shotgun sequence.
ACCESSION   DAOMAA010000420 DAOMAA010000000
VERSION     DAOMAA010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63237_length_1890_cov_2.793460"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="PLO59_06250"
     CDS             complement(<1..585)
                     /locus_tag="PLO59_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00742.1"
                     /translation="MKTIWLLFFNCLAVITFAQNSAFDLIKDVNQKFKSVNSYIAQVQ
                     IKTDVSFIKMLPVQATIYFKQPDKLHLESKGIAMLPKQSPQFFLHNINQNDYLCIEID
                     KQIIDGYNCKGIKVIPLNEGSDLVLATLFIDPLNLVLRKAQITSRSNGTILMLFKYDK
                     YLKQQLPDAVTFELDVAKFKIPKVVAADLENTKTN"
     gene            complement(589..1389)
                     /locus_tag="PLO59_06255"
     CDS             complement(589..1389)
                     /locus_tag="PLO59_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HQV00743.1"
                     /translation="MKPRSYIGFIAIGAIAMLIHIAMWLDGRAYISMVTQSLEQSFNI
                     EGNILNGNLVCFIILQMLIIHVPLLVAMVTGDLMSGEAASGSLRLLATKPITRSQIVC
                     SKFIAGLAYTLILLLFLAIVSWGAAYFIFGSGDLVVLRSDSLVILQADDINWRFVAAF
                     MLAYLSLMLITSLSFMLSCFSNNSIGPIITTMAIMILFTIICSLEIQLFDSIKPWLFT
                     THMASWRLFFDNPIEWPKILNSIYILVIHVVLFFGISLYHFNRKDILV"
     gene            complement(1442..>1890)
                     /locus_tag="PLO59_06260"
     CDS             complement(1442..>1890)
                     /locus_tag="PLO59_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQV00744.1"
                     /translation="ILDEPTTGLDPQGIIDIRNLINYLRQQGKTILLSSHILSEIELI
                     SNRMIIINKGQTVIEGSVTELLSEKALHVTIDVNKPEMAMQLLQQSKFALAQIEIDGT
                     ALKLNIPKDDIAQVNKLLAQANLDVFEISFKRPLESYFLKLTHNNK"
BASE COUNT          626 a          330 c          314 g          620 t
ORIGIN      
        1 atttgttttt gtgttttcaa gatcggcagc cactactttc ggaattttaa atttagctac
       61 atccaattca aatgtaactg catcaggtaa ttgttgtttt aaatatttat catatttaaa
      121 aagcattagt atggtgccgt tgctacggct ggttatttgt gctttacgta gtaccaaatt
      181 tagcgggtca ataaataaag tagccaatac caaatccgaa ccttcattta gtggaattac
      241 tttaatgcct ttacaattat aaccatcaat tatttgttta tcaatctcaa tgcacaggta
      301 atcgttctgg ttgatgttgt gtaagaaaaa ctgtggactt tgttttggca acatggctat
      361 gcccttactt tctaaatgta atttatcagg ttgcttaaag taaatggtgg cttgtaccgg
      421 caacatttta ataaatgaca catcggtttt aatctgtacc tgagctatat aactattaac
      481 cgatttaaac ttttggttta catcttttat caaatcaaaa gcactgtttt gggcaaaggt
      541 tattactgca aggcagttaa aaaaaagtag ccagatagtt ttcatacttt aaactaaaat
      601 atctttacgg ttaaagtgat acagactgat accaaaaaac aacactacat gtattaccaa
      661 tatataaatt gaatttaaaa ttttgggcca ttctatcggg ttgtcaaaaa acaaacgcca
      721 gctagccatg tgtgtagtaa acagccaggg tttaatacta tcgaacaatt gaatttcgag
      781 tgagcaaata atcgtaaata aaatcattat tgccatggtg gtaataatgg gtccaattga
      841 attattgctg aaacaactaa gcataaatga aagcgatgtt ataagcataa gcgataggta
      901 ggccagcata aaagcggcaa caaagcgcca atttatatca tcggcttgta gtatcaccaa
      961 actatcagaa cgaagcacaa ctaaatcacc ggagccaaaa ataaaataag ctgcacccca
     1021 gctaactatt gctaaaaaca ataaaagtat taatgtgtaa gccaaacctg ctatgaactt
     1081 gctacacaca atctgactgc gggtaatggg tttggtagcc aataatctta gcgagcccga
     1141 tgcagcttcg ccactcatta aatcacctgt aaccatagca accaacaggg gtacatgaat
     1201 aattagcatt tgtaaaataa taaaacaaac caaattgcca tttaatatgt ttccttcaat
     1261 attaaatgat tgctcgagcg attgggttac catgcttatg taagcacgcc catcaagcca
     1321 catagcaatg tgaatgagca tagcaattgc accaattgct ataaacccga tatagctacg
     1381 cggtttcatg catattttat aaatctcaac caataataaa ttcaaaaaca ttttcggatt
     1441 tttatttgtt gttatgtgtc agttttaaaa aataactttc caacgggcgt ttaaaactta
     1501 tttcaaaaac atccaagttt gcttgtgcca gtaatttatt tacctgtgct atatcgtctt
     1561 taggtatgtt tagcttaagt gctgtgccat caatttcaat ttgtgccaaa gcaaatttgc
     1621 tttgttgtaa taattgcata gccatttcgg gtttattaac atcaatggta acatgcaaag
     1681 ctttttctga taataattct gttacacttc cttcaataac ggtttggcct ttattgatta
     1741 taatcatccg gttgctgatt aattcaattt cgcttaatat atgtgacgaa agtaaaatgg
     1801 ttttgccttg ttgccttaaa taattaatca aatttctgat gtcaattatg ccttgcggat
     1861 caagacctgt tgttggttcg tcaagtatta
//
LOCUS       DAOMAA010000421         1885 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_63552_length_1885_cov_2.774863, whole genome shotgun sequence.
ACCESSION   DAOMAA010000421 DAOMAA010000000
VERSION     DAOMAA010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63552_length_1885_cov_2.774863"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="PLO59_06265"
     CDS             complement(<1..269)
                     /locus_tag="PLO59_06265"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HQV00745.1"
                     /translation="MHTILVTGTNGLLGQKILYALKKLDVQIIAVAKGPNRTANKYGY
                     HYIDADVTNTTLMTLIFETYRPQTLIHTVALTNVDACETQQDLCW"
     gene            complement(323..544)
                     /locus_tag="PLO59_06270"
     CDS             complement(323..544)
                     /locus_tag="PLO59_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00746.1"
                     /translation="MVERIKTFFEVQAFGVCDWMGDKMGIKSANIRLFFIYTSFITIG
                     SPIIVYLVFAFWLRLKNMVNKQRNSVWDL"
     gene            complement(558..1856)
                     /locus_tag="PLO59_06275"
     CDS             complement(558..1856)
                     /locus_tag="PLO59_06275"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HQV00747.1"
                     /translation="MTEALLHHIWKFKLFNTQNLLTSDGQSVAIMHPGKHNTDQGPDF
                     FDARIKINDTLWAGNVELHLKTSDWQKHFHQNDAAYNNVILHVVFEHDITLIKAPAIQ
                     QQIPVLALNTCVGNHILKQYDNLMLNQYKIPCNNMANAIDGFIWANWHDRLMVERMEH
                     KTQAIESLLIETKNNWEESFYILLARNFGFKINALPFELLARSLPLNCLAKHKNNLHQ
                     IEALLFGQAGLLQNKLMQDYGNALRDEYFFLQKKFELTPLQVHLWKFMRLRPANFPTI
                     RLAQFAQLIHNSSHLFSKLISVNNISDAYACFDVTASAYWNSHFVFDKTIETHIENAS
                     QSRKLGKMAIENIIINTLIPFMFYYGKQKGITAYQQQAFDFLNQIPAEQNHIVKKFTT
                     INLPCTNAYQAQAYIQLYNYYCSHKKCLSCAIGNKIIKND"
BASE COUNT          576 a          314 c          355 g          640 t
ORIGIN      
        1 tgccagcata aatcttgctg tgtttcgcag gcatcaacat tggttaaagc aacggtgtgt
       61 atgagtgttt gtggcctgta ggtttcaaat atcagtgtca tcaatgtggt gtttgtaaca
      121 tcggcatcaa tataatggta accatattta ttagctgtac ggttggggcc tttggcaact
      181 gcaattattt gtacgtctaa cttttttaaa gcatacaata ttttttgacc caatagtccg
      241 ttggtgcctg taactaaaat ggtgtgcata ctaatttaaa cttacaaaat atacatatat
      301 ggtaactgta actaatggtg ggctataaat cccatactga attgcgttgt ttgtttacca
      361 tgttttttaa cctgagccaa aatgcaaaaa ccaaataaac aataatgggc gaaccaatgg
      421 tgataaaaga agtgtaaata aaaaacaaac ggatgttggc cgattttatt cccattttat
      481 cgcccatcca gtcgcaaacg ccaaatgctt gcacttcgaa aaatgtttta atacgctcaa
      541 ccatttaaga ttattctcta atcgtttttg attattttat ttccaatggc acaagataag
      601 cactttttat ggctgcaata gtagttgtac aattgaatat atgcttgtgc ctgatatgca
      661 tttgtacagg gtaaatttat agtggtaaat ttttttacaa tatgattttg ttcagcaggt
      721 atttgattta aaaagtcaaa tgcttgttgc tgatatgcag taatgccctt ttgtttgccg
      781 tagtaaaaca taaatggaat aagggtatta attataatat tttcgatggc cattttacca
      841 agtttgcggc tctgtgatgc gttttcgata tgggtttcga tggttttgtc gaatacaaag
      901 tggctgttcc aataagcgct ggctgttaca tcaaaacagg cataggcatc acttatatta
      961 tttaccgata ttaacttgct aaacaaatgc gaactattat gaataagttg tgcaaactga
     1021 gccaaacgga tagtgggaaa atttgccggg cgtagccgca taaatttcca taaatgaact
     1081 tgcagtggtg ttagctcaaa ttttttttga aggaaaaaat attcatccct caaggcattg
     1141 ccataatctt gcatcagttt gttttgcaat aagccggcct gtccaaaaag taatgcttct
     1201 atttgatgta agttgttttt atgttttgcc aaacaattta atggtaggga gcgagccaat
     1261 agttcgaaag gcaatgcatt aattttaaaa ccaaagttgc gtgccaataa aatataaaaa
     1321 ctttcttccc aattgttttt ggtttcgata agtaaacttt cgatggcttg tgttttatgc
     1381 tccatacgtt caaccataag cctgtcgtgc caattagccc aaataaatcc atcaatagca
     1441 ttagccatgt tattgcaggg tattttatac tggtttagca tcaggttatc atactgtttt
     1501 aaaatatgat tgccaacaca tgtatttagc gctaaaaccg gtatttgttg ttgtatggca
     1561 ggagccttta tcaatgtaat atcatgctca aaaaccacat gtagaattac attgttataa
     1621 gccgcatcgt tttgatgaaa gtgtttttgc caatcgctgg tttttaaatg caactcaaca
     1681 ttaccggccc aaagtgtatc attaattttt atacgcgcat caaaaaaatc agggccttgg
     1741 tcggtattgt gtttgcccgg atgcataata gcaacagact ggccatcgct ggttaataaa
     1801 ttttgggtat taaaaagttt aaacttccaa atatggtgca ataaggcttc tgtcattcac
     1861 actaatattt aatgtgcaag gtaat
//
LOCUS       DAOMAA010000422         1882 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_63726_length_1882_cov_2.642583, whole genome shotgun sequence.
ACCESSION   DAOMAA010000422 DAOMAA010000000
VERSION     DAOMAA010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63726_length_1882_cov_2.642583"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1566
                     /locus_tag="PLO59_06280"
     CDS             <1..1566
                     /locus_tag="PLO59_06280"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV00748.1"
                     /translation="LGVSVVQFLSPLVVTASIFGVFGGAGQTIIKNGISVQVWTQNAA
                     FIWVPWIESLNKGKAHGIRQKAGTNDPITYFIHNYNTGLDTSGTLLTVQMTFYTDTDT
                     VTYYNKWKNDTVLITSDSAEFKIEFPSTLTSLNGHLHVRVGNGVVTLASKTGYFASVP
                     LPVIGTPVPFAFPLQNLLNFNYNLGNFGNDSLQVIINMFGNSINNHTPAGTIHIIDSV
                     KHVPCFGTKTGAIYVNVSGLFPPFTYLWNNGATVQNKTAVKAGTYTVTVTDSQGNTLS
                     KTFVVNQPAKLAASVVKNNVKCGGKNTGSITLNISGGTPAYSVMWSTGETTPHIDSLY
                     AGTYIATITDANGCTLTKAITLTENAPLSIAAFSLGADSAIAFGAGGITPYVYRWFTS
                     PAQSTQTATGLIGGGIYKVRVTDSKSCTATIMYTHPFSKLGVLHEEPVIKITPNPASQ
                     QIAVQISAQKIIGALQLKLKDVTGKTILIQNEKTNELDYKTQLDVRLLTAGIYFIECT
                     TTNSTTIRKFIKE"
BASE COUNT          582 a          394 c          372 g          534 t
ORIGIN      
        1 ttgggcgtgt cggtggtgca gttcttgagc ccgctggtgg tgacggccag catctttggc
       61 gtttttggcg gcgccggcca aaccatcatt aaaaacggca ttagcgtgca ggtctggacg
      121 cagaacgcgg ctttcatttg ggtgccatgg attgaaagtt taaacaaggg caaggcacat
      181 ggcatccgac aaaaagccgg caccaacgac ccgataactt actttataca taactataat
      241 accggacttg atacttcagg tacattatta accgttcaga tgacttttta taccgatacc
      301 gacacagtta cctactacaa taaatggaaa aacgataccg ttttaattac atccgactcg
      361 gccgaattta aaattgaatt tccatcaacc ttaacttcgt taaacggtca tttgcatgta
      421 agggtgggca atggcgtggt aaccctggca tcaaaaacag gctattttgc aagcgtgcca
      481 ttgcctgtaa ttggtacacc tgtgccattt gcatttccgc tgcaaaacct tttaaatttc
      541 aattacaacc tgggcaattt cggtaacgac tcattgcaag ttataataaa catgtttggt
      601 aacagcataa acaatcatac tccggctggc actattcata ttattgacag cgttaaacac
      661 gtgccctgtt ttggcacaaa aaccggagct atttatgtaa atgtttcggg cctgtttccg
      721 ccttttacat atttgtggaa taacggagca accgtacaaa ataaaactgc tgttaaagcc
      781 ggaacctata cggtaactgt tacagatagt caaggcaata cactttctaa aacctttgtt
      841 gtaaatcaac cggccaagct tgctgcatcc gtagtaaaaa acaatgtaaa gtgcgggggt
      901 aaaaatacag gctcgataac tttaaatatt tcaggtggta cacctgcata cagtgtgatg
      961 tggagtacag gagaaaccac acctcatatt gatagtttat acgcaggcac ttatattgca
     1021 accattaccg atgccaatgg ctgtacctta acaaaagcaa ttacgcttac cgaaaatgca
     1081 cctttaagta ttgctgcatt ttcattaggg gccgactcgg ctattgcatt cggtgcgggt
     1141 ggcattacac cttatgtata tcgctggttt acaagtccgg cacaatcaac acaaactgca
     1201 acgggcttaa taggcggtgg tatttataag gttagggtaa ccgatagcaa atcttgtacg
     1261 gctactatta tgtacacaca tccgtttagc aaattaggtg ttttgcatga agagcctgtt
     1321 attaaaatta cacccaatcc tgcaagccaa caaatagcag tacaaataag tgcgcaaaaa
     1381 attataggtg ctttgcaact aaaattaaaa gatgtaaccg gcaaaaccat tctgatacaa
     1441 aacgaaaaaa caaatgaatt ggattataaa acacaattag acgtacgact gctaactgcc
     1501 ggcatctatt ttattgaatg tacaacaacc aacagcacca ctatacgcaa gtttattaag
     1561 gaataaattc atccatttaa attctcgaaa accggcatcc gtagtgtgcc ggttttttat
     1621 tggtcaaagt tttcccattt tcaacgtaac ttaatccatc ttgtgttagc ctgaaatagg
     1681 ttgatttcta tttatcgtaa gttactacac taccggggtt ttgaaaaatg ggcgttcaca
     1741 gggtttgtgg tcaactgcat atctagctga tttttgttac gatttatagt cgcgcaatcg
     1801 gctataataa ctacacaaac aaaaagtttg tagtggtaca acctttttac aagacagctt
     1861 cttgtctgtg caacaaatcg ct
//
LOCUS       DAOMAA010000423         1881 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_63796_length_1881_cov_2.518620, whole genome shotgun sequence.
ACCESSION   DAOMAA010000423 DAOMAA010000000
VERSION     DAOMAA010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63796_length_1881_cov_2.518620"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1521)
                     /locus_tag="PLO59_06285"
     CDS             complement(<1..1521)
                     /locus_tag="PLO59_06285"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQV00749.1"
                     /translation="MSIFKQTIQSMRMHLSAKQKKEMVWLAVLIFIQGVLDVFGLGLI
                     IPVINIASSPEVITQNKFHLGDIYQFFHFTSTNNFILFILFAVLGFYIAKTAFGLFVY
                     WVSAKFATEVAVHITQHQYDKYYAVSYLDFTNIKSSVITNHVFNNPASYLAWVVNPAI
                     IIFSEIVIVTLIVVAIVWVDITLFACIVAVIGPATFMIYYALKNKSALYGVELDKVIP
                     KVYASLNASVNGYIDVKLAGKETDFRSQYMHHIRKYHDIGQKANFIGLVPLRAYEIVA
                     LLSIVVIFVYVLFITAKDASTAVVLVGAFAAAAYRLMPSMNRIVGNLMYIRRSQSTIN
                     NLNYYANHIPGNASKPVDGLIAFDKTIVFDKICYRFPDSDKYVLHNITFEVNKGEKIG
                     FVGASGSGKTTLMNILLRFITEGSGQILIDNTPLTEAHLLNWRKLIGYVKQDIFIMDG
                     TIKENIAFGETEVDDRRLMRAISQASLSDLVRSLPDGVNSVVGEKGSRLSGGQRQRI"
     gene            complement(1571..>1881)
                     /locus_tag="PLO59_06290"
     CDS             complement(1571..>1881)
                     /locus_tag="PLO59_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00750.1"
                     /translation="RTPNASELLLDPSMNKLMDDLMLQFDYIIIDSAPVGLISDSLEL
                     MKYADLTLFVLKANYSKRDFVDVAHQITERNPGKSVGFILNSVSAKNVSAGYGGGYYK
                     "
BASE COUNT          585 a          375 c          335 g          586 t
ORIGIN      
        1 tatgcgttgg cgctggccac cggataagcg gctgcctttt tcgcctacca cactgttaac
       61 accatcgggt aaactacgaa ccaaatctga cagcgatgcc tggcttattg cacgcatcag
      121 cctgcgatca tccacttcgg tttcgccaaa ggcaatgttt tcttttatgg tgccatccat
      181 aataaaaata tcctgcttta cataacctat tagtttgcgc cagtttaata aatgggcttc
      241 tgttaaaggt gtgttgtcta ttaaaatttg cccactgcct tcggtaataa acctgagcag
      301 tatattcatc agcgtagttt tacccgagcc tgatgcaccc acaaaaccaa ttttttcgcc
      361 tttgtttact tcgaatgtaa tgttatgtaa aacatactta tcgctatcgg gaaagcggta
      421 acatatttta tcaaaaacaa ttgttttatc aaatgctatt aaaccgtcaa cgggttttga
      481 ggcattgccc ggtatatgat tggcatagta gtttaaatta ttgatggtgc tttggctgcg
      541 tcttatatac attaaattgc ccactatccg attcatgctg ggcataagtc tgtatgcagc
      601 tgctgcaaag gcacccacaa gtactaccgc tgtgctggca tctttagctg taataaaaag
      661 tacataaaca aaaattacaa caatgcttaa taaggcaaca atttcataag cccgtaaggg
      721 gactaatcca ataaaatttg ccttttgccc aatgtcgtga tacttgcgta tatggtgcat
      781 gtattgactt ctgaaatcgg tttctttgcc ggctaacttt acatcaatat atccatttac
      841 cgatgcattt agtgatgcat atactttagg aataacttta tctaactcaa ccccgtataa
      901 agcgcttttg tttttcaaag cataataaat cataaaagtt gccggcccaa taacggcaac
      961 aatacaggcg aagagtgtaa tgtcaaccca aacaatggca actacaatga gggtaacaat
     1021 tactatttcc gaaaaaataa ttattgccgg atttacaacc catgctaaat aagaagccgg
     1081 attattaaat acgtgattgg taattacgga tgatttgata tttgtaaaat ctaaatacga
     1141 aaccgcataa tatttgtcgt attgatgctg cgttatatgc acagccactt cggtagcaaa
     1201 ctttgccgat acccaataaa caaataaacc aaatgcggtt ttggctatat aaaatcctaa
     1261 aacagcaaac agtataaata gaataaagtt attggttgat gtaaaatgaa agaattgata
     1321 aatatcaccc aagtgaaatt tgttttgtgt tataacttcg gggctcgatg caatattaat
     1381 aactggtatt ataagtccta agccaaacac atccaataca ccctgtataa atattaatac
     1441 agccagccaa accatttctt ttttctgctt ggcacttagg tgcatacgca tactttgaat
     1501 agtttgttta aatattgaca tggtgttggt aacttaaacc ttatacgtag ttttaattgg
     1561 gtgttgtatg ttacttgtaa taaccaccac catagcctgc cgaaacattt ttagcactca
     1621 cactgttaag tataaaccca acactcttac ccggattgcg ttcggttatc tgatgtgcta
     1681 cgtcaacaaa atcgcgtttg ctgtaatttg cttttagtac gaataaagtc aggtcggcat
     1741 atttcattaa ttccaacgaa tcgctgatta agccaaccgg tgccgagtca attataatgt
     1801 agtcgaactg aagcattaaa tcgtccatca atttgttcat actgggatct aataaaagtt
     1861 cgctggcatt gggtgtacgt g
//
LOCUS       DAOMAA010000424         1873 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_64285_length_1873_cov_3.293729, whole genome shotgun sequence.
ACCESSION   DAOMAA010000424 DAOMAA010000000
VERSION     DAOMAA010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64285_length_1873_cov_3.293729"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="PLO59_06295"
     CDS             <1..257
                     /locus_tag="PLO59_06295"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HQV00751.1"
                     /translation="STQQESVTPISNASYYIVAGCFKSDENATAYTKHLLTLGLNAKI
                     IGKRNGLHVVTCFAGSKAETMAALEQIKTKVDAGVWVLKN"
     gene            complement(357..>1873)
                     /locus_tag="PLO59_06300"
     CDS             complement(357..>1873)
                     /locus_tag="PLO59_06300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HQV00752.1"
                     /translation="STNKIASLFQIIDLFYVDTVNEQKVVDDGIRSMLKDLDPHSVYI
                     PADELKETNEPLVGKFEGIGVQFNILQDTIMVTNTIPGGPSEKLGIMAGDRIVNINGE
                     LVAGIKIKNNDVLKKLRGDKGTKVKVDIYRRSEPNLIPFEITRDKIPLFSVDATYMAT
                     PEIGYIKISRFADTTVDEFKEALAKLKTAGMKSLILDLQGNGGGYLNRAIELADEFLP
                     DKKMIVYTEGRAQARQENYATTTGGFEKGKVAILIDETSASASEIVSGAVQDWDRGLL
                     IGRRSFGKRLVQKPYSLPDGSAVRLTIAHYYTPSGRCIQKSYEKGDESYDDDLSNRLK
                     NGELYSRDSIKFNDSLKVYTNGKRVVYGGGGVMPDVFVPLDTTLNSKYWQDLRRKNVP
                     GQWVISYIDENRKDLLAKYPTFDSFKKSFVLDETRMNHFFAFAQKQEVPYDTAGFKTS
                     GLLIKTVLTGSLAQNLYGSVSNYYEIVNNINHSFKEAIRAINDKTFERNKVAAN"
BASE COUNT          522 a          406 c          339 g          606 t
ORIGIN      
        1 cctccacaca acaggaatca gtaacaccaa tttcgaacgc aagttattac attgttgcag
       61 gctgttttaa gtcagacgaa aatgcaacgg cctatacaaa acatttgtta acgttaggtt
      121 taaacgctaa aattattggc aaacgcaacg gacttcatgt agttacctgc tttgcaggta
      181 gcaaagccga aaccatggct gctttagaac aaataaaaac taaagttgat gccggtgtat
      241 gggtgcttaa aaactaaaac ttttctggtt taatttttag gttgctacgt atccgccacc
      301 atcgccaagt ccctctccgt tattaataag tgtttccctt ttgcttaaag ccaaacttaa
      361 tttgcagcaa ctttattgcg ctcaaacgtt ttatcattaa tggcacgtat ggcttcctta
      421 aacgaatggt ttatattgtt tactatttca tagtaattac taacactgcc atataaattt
      481 tgcgcaagcg agcctgtaag cactgttttt atcagtaagc ctgatgtttt aaagcctgct
      541 gtatcataag gcacttcttg tttttgtgca aaagcaaaaa agtgattcat acgtgtttcg
      601 tcaagtacaa atgatttttt aaaagaatcg aaagtggggt actttgccaa taggtcttta
      661 cggttttcat ctatgtaaga tataacccat tggccgggta cattttttct gcgtaagtct
      721 tgccagtatt tgctgtttag tgtggtatct aacggcacaa atacatcggg cattacacct
      781 ccgccaccgt acactacgcg cttaccgttg gtataaactt tgagcgaatc attaaactta
      841 attgaatcgc gcgagtaaag ttctccgttt tttaatcggt tgcttaaatc gtcatcataa
      901 ctttcatcac ctttctcata cgatttttga atgcaacgtc cactgggtgt atagtaatgt
      961 gcaatggtta aacgtacggc actgccatca ggcaacgaat aaggtttttg taccaaccgt
     1021 ttgccaaacg acctgcggcc aataagtaaa cccctgtccc aatcttgtac ggcaccgctt
     1081 acaatttcgc tggccgatgc cgatgtttcg tcaattaaaa tcgcgacctt tcctttttcg
     1141 aaaccacctg ttgtggttgc ataattttct tgccgggctt gtgcacgtcc ttctgtataa
     1201 acaatcattt ttttatccgg caaaaactca tcggccaact caatggcacg atttaaatag
     1261 ccgccaccat tgccctgcaa gtctaatatc agcgatttca taccggctgt ttttagtttg
     1321 gctaatgctt ctttaaactc atcaacagta gtatcggcaa agcgcgatat ttttatataa
     1381 ccaatttccg gtgttgccat ataagtagca tccacactaa acaatggaat tttatcgcgt
     1441 gtaatttcga acggaataag attgggttcg ctgcgtctgt atatatctac tttaaccttg
     1501 gtgcctttat cgccacgaag ctttttcaat acatcattat ttttaatttt tatgccggca
     1561 accaattcgc cattaatatt tacaatgcgg tcgcctgcca taatacctaa tttttcggac
     1621 gggccaccgg gtatggtatt ggttaccata atagtgtctt gcaaaatatt aaactgtaca
     1681 ccaatgcctt caaacttacc taccaatggt tcgttggttt cttttaactc atcggcaggt
     1741 atgtaaaccg agtgcgggtc taagtcttta agcatagagc gtatgccatc gtccaccacc
     1801 ttttgttcgt taactgtatc cacataaaac aagtcaatta tctgaaacaa tgaggctatc
     1861 ttattagtag atg
//
LOCUS       DAOMAA010000425         1871 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_64437_length_1871_cov_2.828194, whole genome shotgun sequence.
ACCESSION   DAOMAA010000425 DAOMAA010000000
VERSION     DAOMAA010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64437_length_1871_cov_2.828194"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1871
                     /locus_tag="PLO59_06305"
     CDS             <1..>1871
                     /locus_tag="PLO59_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00753.1"
                     /translation="PLCETTVHAFTSSWTIEEQNFKSPVLNKFVGYNRLLWVSIAVLI
                     LIFTFCCFSFNQYKIIAGFKKAKQCDNAHAAPVISARVYLSDFDVAHTLIQKLKQLWF
                     ITHTEYMSIVRTGAFASILLAGTLFIYVLLSQMNAPYGNKVLPATWVMLAFPVLFFSL
                     LINFITFLYAGILVKRGVTYGMFYLVDVTYVSNRMLLLSKILALVKLQVTMLSVVCIV
                     GVAVQLSSHFYNIEWWHYFFDLIGIHLIEYVIWACLAIFIQTIFGNIWIGLLILILLF
                     FGGDVVQKLGIENFIFRFNQNPDLGFYLKYLELSGYSHSIAAYFIFKVYWCIASFLIL
                     LLSLMLWQRGMAISYRNRLLLAIHRFKYQFLKPFIIGLLLLVCTGAYIFNARHNAVTT
                     LSEAQKEALLIDTDTMMQRFEFAQQPRIVAVDVAMHIYPEQNMFRSTGTYTLINKTQV
                     PIDTVIITKALDVETQCQLNAPALKLIAHNTVVNVFKLKKQMLPDDTLKLSFEINCMR
                     NNVWYENAFVKSNGTYITSLIYPALGYRPHNYAKQPDDTTALKNHYRSFDSDYIDFKA
                     TVSTAENQTAIAPGCLIKQWKSNQRNYFQYQSTTQVTNDFAFVSGIYKVDTAKYNNI"
BASE COUNT          559 a          309 c          342 g          661 t
ORIGIN      
        1 tcccttgtgc gaaacaactg ttcatgcttt tacatcctca tggacaatag aagagcagaa
       61 ttttaaatca ccggttctta ataaatttgt tggatataac cgtttgcttt gggtaagcat
      121 tgccgtactg attttgattt ttacattttg ctgtttttca tttaatcaat ataaaattat
      181 tgcaggtttt aaaaaggcaa agcaatgtga taatgcccat gcagcacctg ttatttcggc
      241 cagggtgtat ttatctgact ttgatgttgc ccatacatta attcagaaat tgaagcaatt
      301 atggtttata acgcatactg agtatatgag cattgttaga accggtgcgt ttgcaagtat
      361 tcttttagcg ggtacacttt ttatttatgt attgctttcg caaatgaatg caccttatgg
      421 aaataaagtt ttaccggcaa catgggttat gcttgcattt ccggtattgt ttttttcatt
      481 actgataaat tttatcacct ttttatacgc tggcatttta gtaaaacgtg gtgtaaccta
      541 tggcatgttt tacttggttg atgtaactta tgtttctaac cgtatgctgc tactgtcaaa
      601 gatattggct ttggtaaaat tgcaagtaac catgctttca gttgtatgta ttgttggagt
      661 tgccgtacag ctaagcagcc acttttataa tattgaatgg tggcattact tttttgattt
      721 gataggtatt cacttaatcg aatatgtaat atgggcttgc ttggcaattt ttattcaaac
      781 tatttttgga aatatatgga taggtctttt gatattaatc ctgctttttt ttggtggtga
      841 cgtagtacaa aagttaggta tcgaaaattt tatatttcgt tttaatcaaa atcccgacct
      901 cgggttttat ttaaaatact tggagctaag tggatacagt cattctattg cagcatattt
      961 tatttttaaa gtttactggt gcattgcgtc atttttgata ctgctcttaa gccttatgct
     1021 ttggcaacga ggtatggcca tttcgtatcg taacagatta ttacttgcta tacaccgttt
     1081 taagtatcag tttctaaagc cttttattat aggcttgcta ttgcttgtat gtacaggtgc
     1141 gtatattttt aatgcaaggc ataatgcagt tactacttta agcgaagcac aaaaggaggc
     1201 gttactaatt gataccgaca ctatgatgca acgttttgaa tttgcgcaac aacccagaat
     1261 tgtagccgtt gatgtggcaa tgcacattta tcccgaacaa aacatgtttc gttcaaccgg
     1321 aacttacaca ctgataaata aaacgcaagt tccaattgat accgtaataa ttacaaaggc
     1381 tttggatgta gaaacccaat gtcagttaaa tgcgcctgct ttgaagctta ttgcgcataa
     1441 taccgtagtt aacgtattca aattaaagaa acaaatgctg cccgatgata ccctcaagtt
     1501 gtcatttgag ataaattgta tgcgaaataa tgtttggtat gaaaatgcat ttgtaaagag
     1561 caatggcacg tatatcacat ctttaattta tcccgcattg ggttataggc cacacaatta
     1621 cgccaaacag cccgatgata ctactgcatt gaaaaatcat taccgttcat tcgattctga
     1681 ttacattgat tttaaagcta ctgttagtac tgccgaaaat cagactgcaa ttgcacccgg
     1741 ttgcttaatt aagcaatgga aaagtaacca acgaaattat tttcaatatc agtcaacaac
     1801 acaagtaaca aatgattttg catttgtatc gggcatatat aaagtagata cggcaaagta
     1861 taataatatt a
//
LOCUS       DAOMAA010000426         1869 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_64564_length_1869_cov_2.939361, whole genome shotgun sequence.
ACCESSION   DAOMAA010000426 DAOMAA010000000
VERSION     DAOMAA010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64564_length_1869_cov_2.939361"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(205..>1869)
                     /locus_tag="PLO59_06310"
     CDS             complement(205..>1869)
                     /locus_tag="PLO59_06310"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HQV00754.1"
                     /translation="FISSIESVSGKVSKLAAYQTFTGNANYIQQEYSNYQNLTREDIM
                     RVYQTYVKGKNYVALSWYPKGKENRARPDNFLIPEPDAAAPHKDVTVKYSDPKDNFDR
                     SMKPKAGDAPFVTVPDYISQRAGNGMRIIASYTDDVPLINAQIVLAGGHLLDAYDTTK
                     LGLANITARMMNEATKKYTAEQLETALDKLGSSITINCGAEATYVYISSLKKNWAATL
                     DLLSERLLNPAFNADDFERIKKQILEGIANAHTVATSIAEKNYNKILYGNQSIKGSPV
                     DGWQHTVSNITLDDVKSFYNKYYGPDLAQLIVVGDVDVLTLQQQFSFLQTWPKKNYNV
                     PDVTYLSAHKKGTIYFIDKEDAAQSEIRVGYLVMPFDATGEFYRCNVMNFSLGGAFNS
                     RINYTLREEKGWTYGSRSRFNGSKTTGPYTVSGGFRKDATDSAIVEIVNQIKAYKNDI
                     KQSELDFTKSSLSQSEALKYETLNQKANFIGLILDYGLSRDYTTQQYKILNSMQLADM
                     KRLANTYLPIDQMYMLVVGDKKYCFEKLQKLGYPVIELDKEGNIKM"
BASE COUNT          546 a          361 c          312 g          650 t
ORIGIN      
        1 taagtgacga aagttttgga aaagtatttg tgtttgccga cttatatggc aacctatggg
       61 acgttatcga accaactaat aaacaggttt tacttaaaag ctaaaccgta ttcatcaggc
      121 tgatttacca cttttttttc gaatcatatt taattaaaaa gggctgcaaa ttttgcagcc
      181 ctttttaaac ctatatagta aatgttacat tttaatgtta ccttctttat ctaattcaat
      241 aacagggtaa cctaattttt gtaatttttc gaaacaatac ttcttgtcgc caactaccag
      301 catatacatt tggtcaatgg gcaagtatgt atttgccaat cgtttcatat cggctaactg
      361 catactatta agtattttat attgttgggt agtataatcg cgcgataagc cataatctaa
      421 aatcaaacca ataaaattgg ctttttggtt tagggtttca tacttcaaag cctcactttg
      481 cgataacgaa ctttttgtaa aatctaattc agattgctta atatcatttt tataagcttt
      541 tatctgattt acaatttcaa caatggcact atcggttgca tcttttctaa acccacccga
      601 aacagtataa gggccggtgg ttttactgcc attaaacctg gagcgcgaac catatgtcca
      661 acctttttct tcgcgcaagg tgtaattaat acggctgtta aaagccccac ccaaactaaa
      721 gttcatcaca ttacagcggt aaaattcgcc tgttgcatca aaaggcatta ccaaatatcc
      781 cacacgaatt tcgctttgtg ctgcatcttc tttatcaata aaataaatgg ttcctttttt
      841 gtgcgcgctt aagtaagtta catccggtac attatagttt ttctttggcc atgtttgtaa
      901 aaacgaaaat tgttgttgta atgttagtac atcaacatca ccaacaacaa ttagttgtgc
      961 caaatcgggt ccataatatt tattgtagaa tgatttaaca tcatctaaag taatattgct
     1021 aactgtatgc tgccagccat caaccgggct tcctttaata gattggttgc catacaatat
     1081 cttattataa tttttttcgg caatactggt agcaacggtg tgtgcattgg caataccttc
     1141 taaaatttgt tttttaatac gctcaaaatc atcagcatta aatgctggat ttagtaatcg
     1201 ttcgctcagc aaatccaatg ttgctgccca gttctttttt aaagagctta tataaacata
     1261 cgttgcttcg gcaccacaat taatcgtaat ggagcttccc aatttatcta atgcagtttc
     1321 taactgctcg gcagtatatt ttttagtggc ttcattcatc atacgggcag taatatttgc
     1381 cagccctaat ttggtggtat cataagcatc caataaatga ccgcccgcta atacaatttg
     1441 tgcattaatc aaaggtacat catcggtgta tgatgcaata atgcgcatac cgttgcctgc
     1501 tcgttgcgaa atgtaatcag gcaccgtaac aaaaggcgca tcgcctgctt taggtttcat
     1561 actccgatcg aaattatctt ttgggtcgct gtatttaaca gtaacatcct tatgtggtgc
     1621 tgctgcatca ggttcgggaa ttaaaaaatt atcgggcctt gcacggtttt cttttccttt
     1681 aggataccaa cttaatgcaa catagttttt gccttttaca taggtttgat atacacgcat
     1741 tatatcttca cgtgttaaat tttgatagtt gctatactct tgttgtatat aattggcgtt
     1801 accggtaaag gtttggtatg cagctaattt cgacaccttg cctgaaacac tttctattga
     1861 tgatataaa
//
LOCUS       DAOMAA010000427         1865 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_64821_length_1865_cov_3.003315, whole genome shotgun sequence.
ACCESSION   DAOMAA010000427 DAOMAA010000000
VERSION     DAOMAA010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64821_length_1865_cov_3.003315"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1865
                     /locus_tag="PLO59_06315"
     CDS             <1..>1865
                     /locus_tag="PLO59_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HQV00755.1"
                     /translation="MNAWRKNNGNNIQSSGNWSPVGPFVVAPGNGWSPGIGRINGVAV
                     NPLNSNVIYAGAPAGGLWKSNTGGNNFTTTTDHLPAIGFNSIAIDYVDTNIVYALSGD
                     GDAGDTYSLGLLKSMDGGATWNVTGLNWNTINARLGHKVLIDPTDRNIIFVCTNNGIF
                     RSNDAAASFTLVQGGNIFDMEFKPGNSNEMYAVGASSFYKSTNNGVSFSIVTNGLPAP
                     LAANRFAIAVTPANNNYVYLIAGAASNSGFLGLWLSTDAGNTFNFITNTPNVMGWDMD
                     GTDNGGQSWYTLTIAADPLNAAHIITGGVNVWESFDGGNSFNINAHWVINSQSYVHAD
                     IHFLDYINGNLYAGTDGGLFKWDQSALTWLDLSQGLQVSQFYRLATSATDPNFILAGA
                     QDNGSISIINGNSEQWLGADGFEQAINPLNNNIIYGEAQYGGIYRSMDKGATSNYISG
                     GISGSSDWDTPFLLNNIGHLFVGYEDIWRSTNNGNTFSNITNTGLGVLKYMDICESNQ
                     SVMYATNGQNVFKIDLNNGSVTDVTNGLAGTKSYIAVSDIDPNIVYATIGGFTAGAKV
                     FKSTNGGLTWTNISGNLPNIPFNTIIEDTTSSQGVYVGSESGVFYTNNILGNWQL"
BASE COUNT          572 a          316 c          423 g          554 t
ORIGIN      
        1 atgaatgcct ggcgaaaaaa taatggaaac aatatacaaa gcagtggaaa ttggtcgcct
       61 gtaggacctt ttgtagtagc accgggcaat ggttggagcc cgggcatcgg ccgcattaat
      121 ggtgttgctg taaacccgct taatagcaat gtaatttatg caggcgcacc agccggaggt
      181 ttatggaaat caaacacagg cggtaataat tttactacca ctaccgatca tttacctgca
      241 ataggtttta acagcatagc cattgattat gttgatacaa atattgttta tgccttatcg
      301 ggcgatggcg atgccggtga tacttatagt ttaggcttat taaaaagtat ggatggcggt
      361 gccacctgga atgttaccgg tttgaattgg aataccatca atgcaagact tgggcataaa
      421 gtgctaattg acccaacaga tagaaacatc atttttgttt gtacaaacaa tggtattttc
      481 cgaagtaacg atgctgcggc atcttttact ttggtgcaag gcggaaacat ttttgatatg
      541 gaatttaagc ccggcaacag caatgaaatg tatgcagttg gtgcttcaag tttttataaa
      601 tcaacaaaca atggtgtaag tttttcgata gttactaatg gcttaccggc tccattggca
      661 gcaaaccgtt ttgcaattgc cgtaacacct gcaaataaca attatgtgta tttaattgcc
      721 ggagcagcat ctaacagtgg gtttttaggg ctatggctta gtactgatgc cggaaacact
      781 tttaatttta ttaccaatac acccaatgtt atgggatggg atatggatgg tactgataac
      841 ggtggtcaaa gttggtacac attaaccatt gctgccgacc cattaaatgc agcacacata
      901 ataacagggg gcgtaaacgt atgggaaagt tttgatggcg gtaactcttt taacattaat
      961 gcgcactggg ttataaattc acaaagttat gtacatgccg atattcattt tttagattac
     1021 attaatggta atctttatgc aggtaccgat ggtggtttat ttaaatggga tcagagtgca
     1081 cttacatggc tcgatttaag ccaaggcttg caagtgtcgc agttttaccg cttggcaaca
     1141 tcggctacag acccaaattt tattttagca ggcgcgcagg ataatggaag cattagtata
     1201 ataaatggaa acagtgagca gtggttgggt gccgatggat ttgaacaagc aattaatccg
     1261 ctaaacaaca atataattta tggcgaagca caatatggtg gcatttaccg tagtatggat
     1321 aaaggcgcaa ccagtaatta tatttcgggt ggtatttcag gcagcagcga ttgggatacc
     1381 ccatttttat taaacaacat tgggcatctt tttgtaggtt atgaagatat atggcgaagt
     1441 actaataatg gaaatacctt tagcaacatt accaatacgg ggttgggagt attaaagtac
     1501 atggatattt gcgaatcaaa tcaaagtgtg atgtatgcta caaacggcca aaatgttttt
     1561 aaaattgatt tgaacaatgg tagtgtaaca gatgttacca atggattagc aggtactaaa
     1621 agttatattg ctgtatcgga tattgaccct aacatcgtat atgctactat tggtggcttt
     1681 actgccggag ctaaagtgtt taaaagcact aacgggggct taacctggac caatataagt
     1741 ggtaacctgc ccaatatacc atttaacacc attattgaag atactacatc ttcgcaaggt
     1801 gtttatgtgg ggtcggaaag tggcgtgttt tacaccaata atattttagg aaactggcaa
     1861 ttgtt
//
LOCUS       DAOMAA010000428         1865 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_64846_length_1865_cov_2.098895, whole genome shotgun sequence.
ACCESSION   DAOMAA010000428 DAOMAA010000000
VERSION     DAOMAA010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64846_length_1865_cov_2.098895"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="PLO59_06320"
     CDS             <1..328
                     /locus_tag="PLO59_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002698311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQV00756.1"
                     /translation="LTSIGGLTYTTPKGFSGSLRYKHINSRPANEDNSVEAKGYLIFD
                     AVANYKFNKTLTVGLSAENLLNSEWNQAQFDTESRLKGEAQPVSELHFTPGTPFFIKA
                     ILSVNF"
     gene            332..1681
                     /locus_tag="PLO59_06325"
     CDS             332..1681
                     /locus_tag="PLO59_06325"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQV00757.1"
                     /translation="MKNGFLFLGIAILLLGTSVFIMLKQKPRPLFTFVERIEGSTNDA
                     EWLNAKAAIQQLLVVIKKNPQNLKAKQKLANLYINEGRASGNHAYYDAAAMQLIDDIL
                     DEEENNFDALCAQATILLSQHHFNEAEAVGLNLVKQNPHSAYALGVLVDAYVELGKYE
                     KAIAAADKMTALRPDIRSYARIAYLREIIGDYQGAIESMEMAVNAGIRGLEQTEWCRY
                     NLGKLYEHTGKLTLAKNCYELCLLYRNNYAYGNAGLGSIAHIEGNLQTAVTYYKRAKA
                     SVNDFSFSEAMYQIYKSSGQTNFANAELMEAEKLLGANDLNENETHGHYADKELAELY
                     TTANQYDKALTHAMLEYNRRPDNAEVNRTLAWVYFHKNQYKLADELMQVALKPGTQNP
                     ETLLQAAAIKMKLNFKTEASQYIQRAYSVSKICSVNATEILKHIPAQMHLTINSVPK"
BASE COUNT          633 a          333 c          369 g          530 t
ORIGIN      
        1 attaaccagc attggcggct taacctacac cacacccaaa ggctttagtg gttcattgcg
       61 ttacaagcat attaattcac gcccggcaaa tgaagataat agtgttgaag caaaaggcta
      121 tttaatattt gatgctgttg caaattataa attcaacaaa acacttacag ttggtttaag
      181 tgctgaaaat ttattgaaca gtgagtggaa tcaagcacag tttgatactg aaagtcgcct
      241 caaaggcgaa gcgcagcctg tgtcggaact acattttaca ccgggtactc cattttttat
      301 taaagccatt ttatctgtaa atttttaagt catgaaaaac gggtttcttt ttttaggcat
      361 tgcaatttta ttattaggca cttcggtttt tataatgcta aaacaaaaac caagacctct
      421 gtttactttt gtcgagcgta ttgaaggttc aaccaatgat gccgaatggt taaatgccaa
      481 agcagccatt caacagcttt tggttgtaat aaaaaagaat ccgcaaaacc tgaaagccaa
      541 acagaaactt gccaacctat acattaacga aggcagggca tcgggcaacc atgcatacta
      601 tgatgcggca gccatgcaat tgattgacga tatattggat gaagaagaaa ataattttga
      661 tgcattatgc gcacaagcaa ccatactact atcgcagcat cattttaacg aagccgaagc
      721 tgtaggatta aacctggtga aacaaaatcc acatagcgct tatgcattag gagttttagt
      781 agatgcttat gtggaattag gtaaatacga aaaagcaatt gcggcagccg ataaaatgac
      841 agcattacga cccgatataa gaagctatgc acgcatagcc tatctgcgcg aaataattgg
      901 cgattatcag ggcgctattg aaagtatgga aatggctgtt aatgccggta ttcgggggtt
      961 ggagcaaacc gaatggtgta ggtataattt aggaaaattg tatgagcaca ccggcaaact
     1021 tacgctcgct aaaaattgtt atgagctctg tttgctctat cgaaataatt atgcttatgg
     1081 caatgcaggt ttaggtagca tagcacatat agaaggcaat ttgcaaacag cagttactta
     1141 ttacaaacgc gccaaagcaa gtgtaaacga ctttagtttt tcagaagcta tgtatcagat
     1201 ttataaaagc agcgggcaaa caaattttgc caatgccgaa ttaatggaag ccgaaaagtt
     1261 attaggcgca aacgatttaa atgagaatga aacgcatggg cattatgccg ataaagaatt
     1321 ggccgaatta tataccacag ctaaccaata tgataaagcg ttaacccatg cgatgttaga
     1381 gtataaccgc aggcctgata atgcagaggt aaatagaact ttagcgtggg tgtattttca
     1441 taaaaatcaa tacaaattag ccgatgagtt aatgcaagtt gccttaaaac ccggcacgca
     1501 aaatccggaa acattattgc aagccgcagc cataaaaatg aaacttaatt ttaaaactga
     1561 agcatcacaa tatatacagc gtgcttatag cgtttcaaaa atttgctcgg ttaatgcaac
     1621 tgaaatttta aagcatatac ctgctcaaat gcatcttaca ataaatagtg tacctaaatg
     1681 agacattgtt ttttcttact gattttattt ttgtttacca taaatgtcga agggcatact
     1741 ataaactatg cacttgccga tgccgcaaat acaacagttg gttggtacta catgcaatta
     1801 ggttttaccc atatattgcc ctacggttta gatcatatat tgtttgtagt aggattgttt
     1861 ttact
//
LOCUS       DAOMAA010000429         1860 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_65191_length_1860_cov_2.670360, whole genome shotgun sequence.
ACCESSION   DAOMAA010000429 DAOMAA010000000
VERSION     DAOMAA010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65191_length_1860_cov_2.670360"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1690
                     /locus_tag="PLO59_06330"
     CDS             <1..1690
                     /locus_tag="PLO59_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQV00758.1"
                     /translation="WIDLDSYDYLNNKDFLSLDLFAADELINSGLVGYRGYDYTGKNR
                     TDKVSFQDYFSKLDANGNPTREIAPFQPIYMAGFIQDKFAINDLIFNVGVRVDRFDAN
                     QKTVKDKYSIYQTRTAGELNIANRPDNIGDGYVVYVNDVNDPVNADVIGYRDGDTWYD
                     ATGAVLADPGILERQTATGQISPYLANPDDDIQLTTFDVNTSFKDYVPQINVMPRIAF
                     SFPISDMALFFAHYDVLTQRPTSNVALNPRDYFNLAAGTINNADLKASKTIDYELGFK
                     QSLSRSSAITISAFYRELRDMIQQVRVNYAYPFSYTSYGNRDFGTVKGFSITYDLRRT
                     GNVRLSASYTLQFADGTGSNSASNSELINSGQPNLFQVAPLDFDQRHNIVASVDYRYS
                     SGADYNGPMWFGKQFFANAGANFIFRAGSGTPYSATSNIRTEADNIGLQQQGTTQLKG
                     GVNTSRLPWQFRADLRVDKDFMLKAPSGSNQGLVLNVYLQIQNLFDTKNVINVYRATG
                     SPSDDGYLSYAPAQGLINNATDVNAYRDLYDIKVNNPNNFSIPRRMRLGVELQF"
BASE COUNT          583 a          329 c          355 g          593 t
ORIGIN      
        1 ttggattgat ttagattcgt atgactattt aaacaataag gactttttaa gtttagatct
       61 ttttgcggca gacgaattaa tcaactcagg tttagttgga taccgtggat atgactacac
      121 aggcaaaaac cgaactgaca aagtatcttt ccaagattac tttagtaagt tagatgcaaa
      181 cggtaatcca acccgtgaaa ttgcaccatt ccaaccaatt tatatggctg gatttattca
      241 ggataaattt gccattaatg acttaatttt taacgtgggt gtacgtgttg atcgtttcga
      301 tgcaaaccaa aaaacagtta aagacaagta cagtatttat caaacacgta cagcaggcga
      361 attaaatatt gctaatcgtc ctgacaatat tggtgatggt tatgttgtat atgttaacga
      421 tgttaatgat ccggttaatg ctgatgttat cggatatcgt gatggcgata cctggtatga
      481 tgccacaggt gcagttttgg ctgaccccgg aattttggaa cgccaaactg ctacaggtca
      541 gatatcacct tatttggcaa acccagatga tgatattcag ttaacaactt ttgatgttaa
      601 tacaagtttt aaagactatg tgccgcaaat taacgtaatg cctcgtattg cattttcatt
      661 ccctatttct gacatggcat tatttttcgc acactatgat gttttaacac aacggccaac
      721 ttctaacgtt gcgttgaacc cgcgtgatta ctttaatttg gctgcaggca caataaataa
      781 tgccgacctt aaagcatcaa aaaccattga ctatgaattg ggtttcaaac aatctttatc
      841 tcgtagttct gccattacta tttctgcttt ttatcgcgag ctgcgtgata tgattcaaca
      901 agtaagggta aattatgcat atcctttctc ctatacttca tacggaaaca gggattttgg
      961 taccgttaaa ggtttttcaa ttacttatga tttacgcaga acaggtaatg taagattaag
     1021 tgcaagctat acacttcagt ttgctgatgg taccggttct aactctgctt ctaattcaga
     1081 gttaattaat tcaggtcagc ctaatttatt ccaggttgca ccacttgact tcgatcaacg
     1141 tcataatatt gtagcatcgg ttgattacag atatagctca ggagctgact acaatggacc
     1201 tatgtggttt ggcaaacaat tctttgcaaa tgcaggcgct aattttattt tccgtgccgg
     1261 ttcaggaaca ccatatagtg ctacatcaaa catcagaact gaagctgata atattggatt
     1321 gcaacaacaa ggtaccacac aattaaaagg tggtgtaaat acatcgcgtt tgccttggca
     1381 gttcagagct gatttacggg ttgataaaga ttttatgtta aaagcaccaa gcggcagtaa
     1441 tcagggctta gttctaaacg tgtacctaca aatacaaaac ttgtttgata ccaaaaatgt
     1501 tatcaatgta tatcgtgcta ccggcagccc gagcgatgat ggttatttaa gttatgcacc
     1561 tgcgcaaggc ttaattaaca acgctactga tgttaatgca taccgtgatt tatatgatat
     1621 aaaagttaat aatccaaata actttagtat tccacgcaga atgcgtttag gtgttgaact
     1681 acaattttaa ttaattagaa ataaaaaact ctcaataaag atgtcaatta agctaagttt
     1741 taaaataaca accttgataa tgggcttatc cgttattcaa gtggctaatg ctgagaaaaa
     1801 tgtaggtatt agcggtgttg ctaacaaaaa tggaggcaat actacttttg caggttgtgt
//
LOCUS       DAOMAA010000430         1859 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_65253_length_1859_cov_3.054878, whole genome shotgun sequence.
ACCESSION   DAOMAA010000430 DAOMAA010000000
VERSION     DAOMAA010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65253_length_1859_cov_3.054878"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(164..862)
                     /locus_tag="PLO59_06335"
     CDS             complement(164..862)
                     /locus_tag="PLO59_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00759.1"
                     /translation="MKNLTTVFLLLLIAFFQPKAHSVTFMLTPSYAVPPAELPLSRAN
                     HLIDPVEGRAIVNKYHQGGQGAFYTSATLNKIISQKNASGIRGYYAYHNDRMTILIVG
                     ATRQTETRHNDILPGIVALQTGTTVVSPEQNQQVDTGTADTAAFNYRHSAHYNDKIKG
                     GLFGKDAIVALLDQPGCVGVRFYIGLNSSGKKTMVLFGVDAKGVDLVAYVADQNVPCP
                     NYCSDSNSLNTDKR"
     gene            complement(1343..>1859)
                     /locus_tag="PLO59_06340"
     CDS             complement(1343..>1859)
                     /locus_tag="PLO59_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010853873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HQV00760.1"
                     /translation="MQTRLEPYYPILYRGSQGRNAHEMILDCRAFKTSAGIEVTDIAK
                     RLMDYGFHAPTVSFPVAGTLMVEPTESETLDELDRFCEAMISIKKEIEEIENGVASRT
                     DNVLINAPHTAPEVIADAWLHPYTRQQAAYPLGWLAENKFWPSVKRVDNAYGDRNLVC
                     TCLPLAAYEAS"
BASE COUNT          578 a          346 c          364 g          571 t
ORIGIN      
        1 ttaaatgacg ggtaaaatag gttaagcaaa tttggtcgga taagaacgat aatgctaaat
       61 aaaataaaat ccacttaaag ggtttggtaa gtttatcaaa ataccataat ccaagtgcta
      121 aaggaattat agttgtgtaa atagcaatgt atggtaaaac catttatcgt ttgtcagtat
      181 ttaatgaatt tgaatcgcta caataattgg gacaaggcac attctgatcg gcaacataag
      241 caaccaggtc aacgccctta gcatcaactc caaaaagtac cattgttttt tttccgctgc
      301 tgtttagccc aatataaaag cgcacaccta cacagccggg ttggtctaac aaagccacta
      361 tagcatcttt accaaaaagg cctcccttta tcttgtcatt ataatgtgct gagtgcctgt
      421 aattaaatgc agctgtatca gctgttcctg tatccacttg ttggttttgc tcaggtgaaa
      481 ctacagttgt gccggtttgc aaagccacaa tgccaggtaa aatatcattg tgtcttgttt
      541 ctgtttgacg cgtagcgcct actatcagta tagtcattct atcattatga taagcataat
      601 aacctctgat accactggca tttttttgac ttatgatttt atttagagtt gccgaagtat
      661 aaaaggctcc ttggccacct tgatggtact tattaactat ggcacggcct tctacaggat
      721 caatgagatg gttagcacgc gaaagcggta actcggcagg gggtacggca taacttggtg
      781 tgagcataaa agttaccgaa tgtgctttag gttgaaagaa tgcaattagc aataataaaa
      841 atacggttgt aaggtttttc ataataatgg ggtttaataa attgttagga agcacaataa
      901 tatgacattg agaaaaaatt tcaataaata aaaacccttg tcgttacata gggaaaaccc
      961 ttacttaaaa ttcgggaaaa ccatcttgac ctaagctcat tctttgttgt gatttgtatc
     1021 aattgaaaac gatacctgtt cattgcctcg gcataaggtt tctaagcctt tcgttcttct
     1081 tttagtggct attaaacaaa tttaatttac ctacgggggt gagtaatatt aaattagaga
     1141 aacaaagcca ttagtttttt gaggcatgcc ctttggtttg aaacgaaacg attttaatgg
     1201 gcagcacaaa attgaaaccg cttgaaaagc cccgtaaggt ttacaatcgg tcaaaatttg
     1261 tttaattaac taactactaa agacaataaa aaagccacaa acaagtatgg gtttgtggct
     1321 tttttattta atttttaaat catcaagatg cttcgtatgc agctaatggc agacaggtac
     1381 aaaccaagtt tctatcgcca taagcattat caacacgttt aacactgggc caaaacttgt
     1441 tttcggccag ccagccaagt gggtaggctg cttgttgtct ggtgtaggga tgcaaccaag
     1501 catcagcaat aacttctggt gcagtatgag gcgcattaat cagtacattg tcggtacggc
     1561 tggcaactcc attttcaatt tcttcaattt cttttttaat tgagatcatt gcttcacaaa
     1621 aacgatctaa ctcatctaag gtttcgcttt cggtaggttc taccattaaa gtaccagcca
     1681 ccggaaacga tactgtaggt gcatgaaagc cataatccat cagtcgtttg gcaatatcgg
     1741 ttacctcaat gccggccgaa gttttaaaag cgcggcaatc taaaatcatt tcatgtgcat
     1801 tacggccttg gctgcccctg tataaaattg ggtaataagg ttctaaacgt gtttgcatg
//
LOCUS       DAOMAA010000431         1859 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_65276_length_1859_cov_2.417960, whole genome shotgun sequence.
ACCESSION   DAOMAA010000431 DAOMAA010000000
VERSION     DAOMAA010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65276_length_1859_cov_2.417960"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1859)
                     /locus_tag="PLO59_06345"
     CDS             complement(<1..>1859)
                     /locus_tag="PLO59_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00761.1"
                     /translation="NNPIGFACVNQPFNFNQGAIDADGDSLAYELDDPLTASGVPCTF
                     IAGLSKNNPFLTTATGTFTMDPVTGALSFTPNVSQVGVLAVKVKEFRNGNLIGYVRRD
                     IQIIVSTCGNNNLPTASGFNGGNNFSLNICAGQQTCATINSADADANQNVTMTWNNGI
                     PSATFTTNNASKPTGTFCWTPTSADKANNPHIFTVTVKDDACPNNGVQVFSYTINVVD
                     LSITTSKTNASCGGNNGSATVTAVPANGSTYLWSNGKTTAANNNLAAGTYTVTVTSSA
                     GCTKTASVVVGNNNQGINTSGNVTNASSGCNGAIDLTVNGGTAPYGYNWSNGATSEDI
                     SSLCAGTYTVTVTDANGCTGSSSYTVTGGSGCNLGLSLKGNDEGTCKQGGIIYAYLTG
                     FTGTGSASLYKNNIFVKTISIKNDFAFDRLSNGSYTVMVTDGGNGCTASATTNIGVLG
                     CGAIVDLAVTLIKNTRATVSWRACGATKHTIRYKENHPNAPFMYRTVNLANQAILKNL
                     NPNTTYSVRVKSQCTTGTAPYSLQELFCTLPCITPKLGNIDDNVTEMVDDNTEAGMNG
                     IFDVMPNPATDVVNVIGETSIEGQATIEVTDLMGRVMLTQTAIVDDENFEANI"
BASE COUNT          539 a          374 c          349 g          597 t
ORIGIN      
        1 ttgatattag cctcaaaatt ttcatcgtca actattgccg tttgggtaag cattacacgg
       61 cccattaagt cagtaacttc aattgttgct tgtccttcga tactggtttc tccaattaca
      121 ttaaccacat ctgttgcagg atttggcatc acatcgaaaa tgccattcat accggcttct
      181 gtgttgtcgt caaccatttc ggttacatta tcatcaatgt tacctaattt aggggttata
      241 catggcaagg tgcaaaacag ctcttgaagc gaataaggtg cagtgccagt agtacattgc
      301 gacttaactc taaccgaata agtagtattt gggtttaggt tcttaagaat tgcctgattt
      361 gctaagttta cagtacgata cataaacggt gcattcggat ggttttcttt gtaacgaatg
      421 gtatgttttg ttgcaccgca agcacgccaa gaaacagttg cacgggtgtt tttaattaat
      481 gtaactgcta aatctacaat ggcaccacaa cctaaaactc caatattagt tgttgcactt
      541 gcagtacatc cattacctcc atcagtaacc atcactgtgt aagagccatt gcttaaacga
      601 tcaaaagcaa agtcgttttt aatcgaaatg gttttaacaa aaatgttgtt tttatacaaa
      661 ctggcactgc ctgttccggt aaatcctgtt aagtatgcat aaataatgcc tccttgttta
      721 caagtacctt catcattacc ttttaagctt aagcctaaat tacagccact accacccgtt
      781 acagtataac tgcttgaacc cgtgcaacca tttgcatcgg taactgttac tgtataggtt
      841 ccggcacata gtgaactaat atcttctgag gttgctccgt ttgaccaatt ataaccataa
      901 ggagcagtac caccgttaac cgttaaatca atggctccgt tacatcctga agaagcatta
      961 gttacattac cagatgtgtt aataccttga ttgttattgc ccacaacaac cgatgcagtt
     1021 tttgtacagc cggcactact tgtaaccgtt acagtatatg taccggcagc taaattgttg
     1081 ttggcagcag tagttttacc gttagaccat aagtaagttg agccattggc tggtacagca
     1141 gttacagtag ccgaaccatt attgccacca caacttgcat ttgttttaga tgttgtaata
     1201 cttaagtcaa ctacattaat agtataagaa aaaacctgaa caccgttatt ggggcaagcg
     1261 tcatctttaa cagttactgt aaaaatgtgt ggattgtttg ctttatcagc tgatgttggt
     1321 gtccaacaaa aagttccggt tggttttgat gcattattgg tggtaaacgt tgctgaagga
     1381 attccgttat tccaagtcat agtaacgttt tgatttgcat ctgcatcggc tgagtttata
     1441 gttgcacaag tttgttgacc ggcacaaatg tttaagctaa agttatttcc accgttaaaa
     1501 ccagatgctg taggtaaatt gttattacca catgttgaaa ctataatttg tatatcgcgt
     1561 cttacatagc ctattaggtt tccgtttctg aactctttaa cctttacagc taacacacct
     1621 acctgactta cattgggtgt aaaacttaaa gctccggtta ccgggtccat agtaaatgtg
     1681 ccggttgcag ttgtcaaaaa tggattgttt tttgataatc ctgcaataaa agtacacggt
     1741 actccgcttg cagtcaatgg atcatctaat tcataagcta atgaatcgcc atcggcatca
     1801 atagcgcctt ggttaaaatt aaaaggttga ttaacacagg caaaacctat agggttatt
//
LOCUS       DAOMAA010000432         1856 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_65463_length_1856_cov_2.857857, whole genome shotgun sequence.
ACCESSION   DAOMAA010000432 DAOMAA010000000
VERSION     DAOMAA010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65463_length_1856_cov_2.857857"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1160)
                     /locus_tag="PLO59_06350"
     CDS             complement(<1..1160)
                     /locus_tag="PLO59_06350"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HQV00762.1"
                     /translation="METKKLFLLDALALIYRAYFAFSKNPRINSKNVNTSAMFGFTNT
                     LLDVLNNENPTHIAVAFDTSAPTARHIDFENYKAHRQEMPEDLRISIPIIKNIIRAFN
                     IPVIELDGYEADDIIGTLAKRAEQENFEVFMMTPDKDYGQLVTEKIKMYKPAVFGNPP
                     EVVGIEGILKKFDIKEISQVIDILGLMGDAADNIPGIPGVGEKTAAKLLHEYGTLENV
                     LLNADKVKGKLGEKIRDNAQQAILSKQLATIILDVPVTFDETTLLRQPPNEEVLREIF
                     NDLEFKQLTKRVFAQSTEPASTPTNTTSQQNNLFDTEPAQPIVTRASVQLATINDVPH
                     QYHICDTEVLQQQLANSLMQQTEFCFDTETTGIDANEAELVGMSFSFKKGEA"
     gene            1364..>1856
                     /locus_tag="PLO59_06355"
     CDS             1364..>1856
                     /locus_tag="PLO59_06355"
                     /inference="COORDINATES: protein motif:HMM:NF017046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HQV00763.1"
                     /translation="MKYKALPASLYIENRKRLVAQLPTKSLAIFNSNDEMPRVGDQTH
                     DYRQNSDLLYLSGIDQESSILILAPNHPLVAYREMLFLKKTSALIAIWEGHKYTIEEA
                     QAQSGIQTIMWAEDFKNILPVLMHHSNEIFLNSNENDRYVHSVPYADLRFIHQLQQTY
                     PLHR"
BASE COUNT          526 a          350 c          352 g          628 t
ORIGIN      
        1 aatgcttcgc cttttttaaa gctaaaactc atgcctacca actcagcctc attggcgtca
       61 atgcctgttg tttcggtatc aaaacaaaac tctgtttgtt gcattaatga atttgccaac
      121 tgctgttgca gcacttccgt atcgcaaata tgatattgat gtggcacatc gtttatagta
      181 gccaactgca cacttgcacg cgttactatg ggttgcgcag gctcggtatc aaacaggtta
      241 ttttgttgtg atgtggtatt tgttggcgtt gatgcaggtt cagttgattg tgcaaacaca
      301 cgtttggtaa gttgcttaaa ttctaagtcg ttaaaaattt cgcgtagtac ttcttcattt
      361 ggtggctgcc tcagtaaagt tgtttcgtca aaagtaacgg gcacatctaa aataatggtg
      421 gccaattgtt ttgataaaat tgcttgttgt gcattgtctc ttattttttc gcccagttta
      481 cctttcactt tatcggcatt gagcaaaaca ttttcaagtg taccgtactc atgcaataat
      541 ttggctgctg ttttttcgcc tacaccgggt atgcctggta tattgtcggc agcatcgccc
      601 attaagccta aaatatcaat tacctgtgaa atttctttga tgtcgaattt ttttaaaatg
      661 ccttcaatac ccactacttc gggcggattg ccaaacacag ccggtttata cattttaatt
      721 ttttcggtaa ccaactggcc gtaatcttta tcaggtgtca tcataaacac ttcaaaattt
      781 tcttgttcgg cacgtttggc caatgtgcct ataatatcat cggcctcata gccatccaat
      841 tcgataacgg ggatattaaa cgctcggata atatttttaa taatgggtat agaaatgcgc
      901 aaatcttcgg gcatttcctg gcggtgtgct ttgtagtttt cgaaatcaat atgccttgcc
      961 gttggtgccg aagtatcaaa tgctacggct atgtgtgtag ggttttcgtt atttaaaaca
     1021 tccaataatg tattggtaaa accaaacatg gccgatgtgt ttacattttt tgaatttata
     1081 cgtgggtttt tgctaaaagc aaaataggcg cggtatatta atgccaatgc atcaagcaga
     1141 aacaattttt tggtttccat aaatttaaaa tgttagggta cgaatgtatt attttgaaaa
     1201 agtactttaa tagcctaatg cattctgcgc attgtatttg ttgttgcgtt tatactaact
     1261 cactgtgttt cgtataacta cttttaatag ctatttaaga attaaagtgt atgggctgtt
     1321 ttatttttta cttttttgca ctaacaattt aaaactcttt aatatgaaat ataaagcact
     1381 gcctgcatcg ttgtatatag aaaaccgtaa acgcctggtg gcacaattgc caacaaaatc
     1441 gttagccata tttaacagta atgatgaaat gccgcgcgtt ggcgaccaaa cacatgacta
     1501 ccgccaaaat tctgatttgc tttatctaag tggcattgat caggaatcgt caattttaat
     1561 attagctcct aatcatcctt tggttgccta tcgcgaaatg ttgtttctga aaaaaaccag
     1621 tgcattaatt gccatttggg aggggcataa atacaccatt gaagaggcgc aggcgcaatc
     1681 aggtattcaa accattatgt gggccgaaga ctttaaaaac atactgccgg tattaatgca
     1741 tcacagcaat gaaatatttt taaacagcaa tgaaaacgat cgttatgtac acagtgtacc
     1801 ttatgcagat ttaaggttta tacatcagct tcaacaaacg tacccattgc atcgct
//
LOCUS       DAOMAA010000433         1855 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_65523_length_1855_cov_3.079444, whole genome shotgun sequence.
ACCESSION   DAOMAA010000433 DAOMAA010000000
VERSION     DAOMAA010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65523_length_1855_cov_3.079444"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            256..1758
                     /gene="atpD"
                     /locus_tag="PLO59_06360"
     CDS             256..1758
                     /gene="atpD"
                     /locus_tag="PLO59_06360"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015809218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HQV00764.1"
                     /translation="MMQKGRVVQVIGPVVDVAFDGGAKLPKIFEALEIDRPDGSKLIL
                     ECQQHIGQDIVRTVSMDSTDGLVRGTEVLAKGAAISMPVGEAIKGRLFNVVGDSVDGM
                     TTVSKENGLPIHRQAPSFEQLTTESDVLYTGIKVIDLIEPYVKGGKIGLFGGAGVGKT
                     VLIMELVNNIAKAYSGLSVFAGVGERTREGNDLLREFIESGVINYGDEFKHSMEKGGW
                     DLSKVDYNKLKESKATLVFGQMNEPPGARARVALSGLTIAEYFRDGDGSGKGKDILFF
                     IDNIFRFTQAGSEVSALLGRMPSAVGYQPTLATEMGAMQERITSTKNGSITSVQAVYV
                     PADDLTDPAPATTFAHLDATTVLNRKIAELGIYPAVDPLDSTSRILSAAVLGEAHYRC
                     AERVKNILQRYKELQDIIAILGMDELSDDDKLVVSRARRVQRFLSQPFHVAEAFTGLK
                     GVLVPIEDTIKGFNMIMDGEVDQYPEAAFNLVGSIEDAIEKGKKLLAEAN"
BASE COUNT          562 a          330 c          429 g          534 t
ORIGIN      
        1 tggcacaagg cattaagttt gatatttaat cgcataaaag cattgtaaaa ggccctgtat
       61 tggggctttt tttattttga ttgcaaccta aagccatcaa attaatacag ccgggaaaat
      121 acttttttga gtaataaata tttttacctt cgcgcccgat tttaaaaggc ctcattaaaa
      181 gccattaaag cttaaaaaat aaaggttttt atagtcggtt aaaatcagtc aaatttataa
      241 atcaccaaac catacattat gcaaaaaggt agagtagttc aggtaattgg tccggtagtg
      301 gacgttgcct ttgacggagg tgcaaaactt ccaaaaattt tcgaagcact tgaaattgat
      361 cgtcctgatg gttctaaact tatattagaa tgccagcagc acattggtca ggatatcgtg
      421 cgtaccgtat ccatggactc gaccgatggt ttggtacgtg gtaccgaagt gttggcaaaa
      481 ggcgcagcta tttcaatgcc tgttggcgaa gccataaaag gccgcctgtt taatgttgta
      541 ggcgattcgg ttgatggtat gacaacggta agcaaagaaa acggcttgcc tattcaccgt
      601 caggccccat cgttcgaaca acttactacc gaaagcgatg tattatatac cggtattaaa
      661 gtaattgacc tgattgagcc ttatgtaaaa ggaggtaaaa ttggtttgtt tggtggtgcc
      721 ggtgtaggta aaaccgtatt gattatggaa ttggtaaaca atattgccaa agcatattca
      781 ggcttatcgg tatttgccgg tgtgggcgaa cgtacccgcg aaggaaacga cttattacgt
      841 gagtttattg aaagtggcgt aattaactat ggtgatgagt ttaagcattc gatggaaaaa
      901 ggtggttggg atttatcaaa agttgattac aataaattaa aagaatcgaa agcgaccttg
      961 gtgtttggtc agatgaatga acctccggga gcccgtgcac gtgtagcact aagcggatta
     1021 accattgccg agtattttcg cgatggcgat ggtagcggta aaggaaaaga catcttattt
     1081 tttattgata acattttcag gtttacacag gcaggttcag aggtgtcggc tttattaggt
     1141 cgtatgcctt cggccgtggg ttatcagcca acattggcta cagaaatggg tgccatgcag
     1201 gagcgtatta catcaaccaa aaacggttca attacatcgg tacaagcagt atatgttcct
     1261 gctgacgatt taactgatcc ggctccggca actacatttg ctcacttaga tgctactaca
     1321 gtattaaacc gtaaaattgc cgagttaggt atttatcctg ccgtggatcc attagattct
     1381 acatcgcgca ttttatcggc tgcagtttta ggcgaagcac attatcgttg tgctgagcgt
     1441 gtaaaaaaca tattacaacg ctataaggag ttacaggata ttattgccat cttaggtatg
     1501 gacgagttaa gtgatgatga taaattggta gtaagccgcg cacgcagggt acaacgtttc
     1561 ttatcgcaac ctttccacgt tgccgaagcg tttaccggat taaaaggcgt gttggttcct
     1621 attgaagaca ccattaaggg atttaacatg attatggatg gcgaagttga ccaatatcct
     1681 gaagcagcat ttaacttagt aggttcgata gaagatgcca ttgaaaaagg taaaaaactg
     1741 ttagccgaag ccaactaatt tttttaagac taaaacaaca atacccatgt acgtagaaat
     1801 aataacacct gataaaatgg tatttgccgg agagtgtaca gccgttcagg tgccg
//
LOCUS       DAOMAA010000434         1855 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_65532_length_1855_cov_2.793889, whole genome shotgun sequence.
ACCESSION   DAOMAA010000434 DAOMAA010000000
VERSION     DAOMAA010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65532_length_1855_cov_2.793889"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1129
                     /locus_tag="PLO59_06365"
     CDS             <1..1129
                     /locus_tag="PLO59_06365"
                     /inference="COORDINATES: protein motif:HMM:NF024107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV00765.1"
                     /translation="AFLVNAMQGQSLMKNNLLTGRINRKSCIIGHNMGGGCALLASSY
                     NKNITTYITLAPAETNPSAVNAATKIYVPVLNITAGNDCVTPASENASPMYEALSGNC
                     KMLINIKGGSHCQFANFNNNCNAAEQGCSVAAISRTQQQSIAMRYILLWLDFRLKNNC
                     TAHFTLMDETANDNAIETRQACNNAVVCVSPANKTATDIGPTTALLKWKANTCAATYE
                     LRYKNSNVLQWISLGTIGAVTSYMLTGLTPGMSYDWSLRTICDDDATTISTWGNKKTF
                     TTATQQLIGFNAENKQDVSFDINPNPSFGKFSMAASINALKPIVISVYSSIGKKVSET
                     TIMPKETELTHLVDLSNESSGIYFVRINYGDIIDTQRIVKR"
     gene            1180..>1855
                     /locus_tag="PLO59_06370"
     CDS             1180..>1855
                     /locus_tag="PLO59_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HQV00766.1"
                     /translation="MATIIINIGKLLGLHANQPLKIPAVNDVAYIDNAWLLITDGKIK
                     QWGSGTTWQAAAIAASQIIDAEGGWIMPAFCDSHTHIVFDAWREKEFEDRINGLTYQE
                     IFARGGGILNSVERLRKADEVKLYDDAYQRLYSMMLQGTGAIEIKSGYGLSLESELKM
                     LRVIRKLKNQTPVIIKSTFLGAHAVPVEFKNDMAGYIKHIIDDMLPAIADEGLADFCD
                     VFCEQDY"
BASE COUNT          612 a          352 c          370 g          521 t
ORIGIN      
        1 ggcattcttg gttaatgcca tgcaaggaca gagcttaatg aaaaacaatt tacttaccgg
       61 gcgcattaat cgcaaatcat gcattattgg tcataatatg ggtggcggtt gtgcactgtt
      121 agcatcaagc tataataaaa acattacaac ctatatcact ttagcaccgg ccgaaaccaa
      181 tccatctgca gttaatgcag ccacaaaaat ttatgtgccg gttttaaaca ttacagccgg
      241 caacgattgt gttacaccgg cttccgaaaa tgcaagtcct atgtatgaag cattatccgg
      301 aaattgtaaa atgcttataa acataaaagg cggcagccat tgccagtttg caaactttaa
      361 caacaactgt aatgctgccg agcaaggttg cagtgttgct gcaataagcc gaacccaaca
      421 acagtcaata gccatgcgct atatattgct gtggttagat tttcgtctga aaaataattg
      481 cactgcacac tttacgttga tggatgaaac cgcaaatgac aatgcaatag aaactcggca
      541 agcctgtaat aatgcggtag tttgtgtatc gccagcaaat aaaactgcta ctgacattgg
      601 cccaactact gcattactta aatggaaagc taacacctgt gccgcaacct atgaattacg
      661 ctataaaaac agcaatgtct tgcaatggat atcgttaggt acaattggtg ctgtaaccag
      721 ttatatgcta acaggtttaa caccgggcat gagttacgat tggtcattac gtacaatttg
      781 tgatgatgat gcaacaacca tatcaacctg gggtaataaa aaaacattta ctactgcaac
      841 ccaacaatta attggcttta atgctgagaa taaacaagat gtatccttcg atatcaatcc
      901 caatccttca tttggtaagt tctcaatggc tgcatcaatt aatgcactta agcccatagt
      961 tatttctgtg tatagcagta tcggaaaaaa agtaagcgaa accactataa tgcccaaaga
     1021 aacagaactt acccatttag ttgatttaag caacgaatcg tcaggtattt attttgtgcg
     1081 cattaattac ggtgatataa ttgacacaca gcgcattgta aaacgctaat tttttaaaat
     1141 taaactgtaa agcgcaatac attttatttg ctttgcaaca tggcaactat cattattaat
     1201 attggtaagc tgttaggttt acatgcaaat caaccactga aaataccggc cgtaaacgat
     1261 gtggcataca ttgacaatgc ctggctttta attacagatg gaaaaataaa acaatggggc
     1321 agtggtacca cgtggcaagc agcggcaatt gctgcatcac aaataattga tgccgagggc
     1381 ggatggatta tgcctgcatt ttgcgattcg cacacacata tagtttttga tgcctggcgc
     1441 gaaaaagaat ttgaagacag aattaacggc ttaacctatc aggaaatttt tgcccgtgga
     1501 ggcggcatac ttaattcagt tgaaaggctg cgaaaagctg acgaagtaaa attatatgac
     1561 gatgcatatc agcgtttgta cagcatgatg ttgcaaggca ccggagccat tgaaataaaa
     1621 agtggatatg gtttaagcct tgaatcggaa ttaaaaatgc tgcgtgttat cagaaaactt
     1681 aaaaaccaaa ctccggtaat tattaagtca acttttttag gcgctcatgc tgtacctgtt
     1741 gaatttaaaa acgatatggc tggttacatc aaacatataa ttgatgatat gttaccggcc
     1801 attgccgatg aaggcttggc cgatttctgt gatgtttttt gtgagcaaga ttatt
//
LOCUS       DAOMAA010000435         1851 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_65828_length_1851_cov_2.438753, whole genome shotgun sequence.
ACCESSION   DAOMAA010000435 DAOMAA010000000
VERSION     DAOMAA010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65828_length_1851_cov_2.438753"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..>1851
                     /locus_tag="PLO59_06375"
     CDS             135..>1851
                     /locus_tag="PLO59_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013622145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HQV00767.1"
                     /translation="MKKYLINILAPLTLMLWCTYANAQIVEPIHWSFSVEQVNASEAT
                     LVLKAKIDPPYHIYSQSNVGGIGLPLEFKFDKTANYELVGKVTEPKAKEHYEESEKVT
                     LKYFEKEATFRQKIKIKTDKPFQITGMATHQACNDEGCLAPTDVDAVFKINAHATNDS
                     KSDVAPASPVIDTASTQQPVDSGSTQVSNQTPTTQANFAANFTLKNDASILEPECGNT
                     KAADETTTLWATFWLAFIGGFLALLTPCVFPMIPLTVSFFTKRSPTRATGLRNAFIYA
                     LSIIVIYVLLGLLITILFGSDALNDLASNGYMNMIFFVVFVIFALSFFGAFEIVLPSS
                     WVNKADQKSEQGGLVGIFFMAFTLALVSFSCTGPIIGSLLVTASRGGYLGPAIGMFGF
                     SFALALPFALFAAFPGWLNTLPKSGGWLNTVKVVLGFLELALALKFLSNVDLAYHWDF
                     LKREIFIALWIIIFGLCGIYLLGKLRFSHDSETKYTSTGRLIFGILFLSFTVYLVPGL
                     WGAPLRLISGFPPPEFYKEWVTSHNECPHNLTCFHDLDEGVAYARAQNKPIMIDFTGW
                     NCVNCR"
BASE COUNT          542 a          361 c          370 g          578 t
ORIGIN      
        1 aaaaggacaa ggaagtttag atgcactttt acgtggccgc gatacctggg aaatagttta
       61 aactatccaa ccatatttat ttatacatac catgctcaac agcggcaact gaagttgatt
      121 taatgatttt acgattgaaa aaatatttaa taaacatcct cgctccttta actttaatgc
      181 tttggtgtac ctatgccaat gcacaaattg tagagccaat acattggagt tttagtgttg
      241 aacaggttaa cgcatccgaa gcaacattag ttttaaaagc aaaaatagat ccgccctatc
      301 atatatactc acaaagtaat gtgggcggca ttggattgcc tttagagttt aaatttgata
      361 aaacagcaaa ctacgaattg gttggcaaag taaccgaacc aaaagctaaa gagcattatg
      421 aagagtcaga aaaagtaaca cttaaatatt ttgaaaaaga agccacgttt cgtcaaaaaa
      481 taaaaattaa aaccgacaag ccatttcaaa ttacaggcat ggctacacac caagcctgca
      541 atgacgaagg ctgtctggct ccaaccgatg ttgatgccgt ttttaaaata aacgcccatg
      601 caaccaacga ttcgaaaagt gatgtagcac cggcttcacc ggttattgat acggcttcaa
      661 cgcaacaacc ggttgatagc ggtagtaccc aagtaagcaa tcaaacacca accacgcaag
      721 caaactttgc tgctaatttt actttaaaaa acgatgcatc tattttagaa cctgaatgtg
      781 gcaacaccaa agcagccgat gaaactacca ctctttgggc tactttttgg ctggcgttta
      841 taggtgggtt tttggcactg cttacaccct gcgtgtttcc gatgattcct ttaacggtaa
      901 gctttttcac aaaaagaagc cccacaagag ctaccggctt acgcaacgca tttatttatg
      961 cattaagcat tattgtaatt tatgttttgc tggggctatt aatcaccatt ttatttggtt
     1021 ctgatgcatt aaacgatttg gcaagcaatg gttatatgaa catgatattt tttgtagtgt
     1081 tcgtaatatt tgcactatca ttttttggtg cattcgaaat agttttaccc agttcatggg
     1141 taaacaaagc cgaccaaaaa agcgaacaag gcggtttggt tggcatcttt tttatggcct
     1201 ttactttggc attggtatcg tttagttgta ccgggcctat tattgggtcg ttgctggtaa
     1261 cagcatcgcg cggtgggtat ttggggcctg caattggcat gtttggtttt tcatttgcat
     1321 tagccctacc ctttgcattg tttgccgctt ttccgggatg gcttaatacc ttaccaaaat
     1381 caggtggctg gctcaataca gttaaagtag ttttggggtt tttagaacta gccctggcac
     1441 ttaaattttt atcgaatgtt gatttagctt accactggga ttttttaaag cgcgaaattt
     1501 ttattgccct ttggattatc attttcgggt tatgcggcat ttacttatta ggtaagttac
     1561 gtttctcaca cgatagtgaa accaaataca catcaacggg cagactaatt ttcggcatcc
     1621 tatttttatc atttacggtt tatctggtgc cgggtctttg gggcgcacct ttacgattaa
     1681 taagtgggtt tcctccaccc gagttttata aagaatgggt aacctctcat aatgaatgtc
     1741 cacataattt aacctgtttt cacgaccttg atgaaggtgt agcttatgca cgtgcgcaaa
     1801 acaaacccat catgattgat tttacaggtt ggaactgtgt aaactgcagg c
//
LOCUS       DAOMAA010000436         1848 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_66013_length_1848_cov_3.674289, whole genome shotgun sequence.
ACCESSION   DAOMAA010000436 DAOMAA010000000
VERSION     DAOMAA010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66013_length_1848_cov_3.674289"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="PLO59_06380"
     CDS             complement(<1..446)
                     /locus_tag="PLO59_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00768.1"
                     /translation="MNLCQKSIGVLFCMLFFQLTLAQNNASTLNQFLQTFNDTATVAS
                     DAAPMQADEIMAKKWNQWQTKYFSLNLGLALFLDYNLTEQNETSKTQVGSIADAVEFR
                     AQRLILSGNLFLTKRPWRYMISFNYNGMDAPQGSKAFSLIDLNLEIP"
     gene            656..1351
                     /locus_tag="PLO59_06385"
     CDS             656..1351
                     /locus_tag="PLO59_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00769.1"
                     /translation="MDNSNTPPNNARFIYPNFKLIFHNYQFYNTAFDEQADSFLFDQD
                     TSQLQLIFEAENALALLAKKDTLHLQAYVFNALGGIQIQVIPNRKTDRFKVAFALGEE
                     IIETFDNSKFNRVDYESSEAYTQAIQNWDAGKVFYKAISKYQGVNDSAKYFFQLPAID
                     LNVYEQKIKQRLHLKDTLVDRSGESENIATIIYKNKACRYFINHVYLRINRYADKKLI
                     EAKHIVIEIYYGC"
     gene            complement(1468..>1848)
                     /locus_tag="PLO59_06390"
     CDS             complement(1468..>1848)
                     /locus_tag="PLO59_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00770.1"
                     /translation="GVISFINDLDNKPQQKIMWALHNMFVSYPQISGKVKFNIPFYYH
                     TTWVCYLKPIKPDAVEVCFMRGFDIQLYPKILLSKNRSMVKGITVTKLTPKLMRQVET
                     VFIEAIEVAKKTPFSVAHYKQHKM"
BASE COUNT          589 a          331 c          315 g          613 t
ORIGIN      
        1 ggaatttcta aattcagatc tattaaacta aaagccttgc tgccttgggg tgcatccatc
       61 ccattgtaat taaacgatat catataacgc cacggccttt ttgtcaaaaa taaattaccg
      121 gataaaatca agcgctgtgc cctaaattca acagcatcgg caatagaacc aacttgagtt
      181 ttgctggttt cgttttgttc tgttaaatta taatccagga ataatgccaa gcccaaattc
      241 aaactaaaat atttggtttg ccactgattc cattttttag ccataatttc atcagcttgc
      301 atcggtgcag catcgcttgc aacagttgct gtatcattaa acgtttgtaa aaactggtta
      361 agtgttgatg cgttattttg tgctaaggtt aattgaaaaa ataacatgca aaataaaacg
      421 cctattgatt tttggcatag gttcatttga aaagtgagtt tatgtttaga agcgttttca
      481 aattaactac tttattgttt tgttttaaac ctttttaaat ctcgttttat tactttagcg
      541 ttcagcatct gtttggataa ttgctagaac taacgtgaaa caacatgctt ttaaatttca
      601 ttacctcttt gtaattctga tgttattgca aggccttgct ttctgccaat acgaaatgga
      661 taatagcaac acgcctccca ataacgcccg ttttatttac cctaacttta agttgatttt
      721 tcataattat cagttttaca acacggcatt tgatgagcag gcagacagtt ttctttttga
      781 tcaggataca tcgcaacttc aactaatttt tgaagccgaa aacgctttgg ctttgcttgc
      841 taaaaaagac accctgcatt tgcaagcgta tgttttcaat gcattaggcg gcattcaaat
      901 tcaagtaatt ccgaatagaa aaaccgaccg gtttaaagtt gcatttgcat taggcgaaga
      961 aattatagaa acttttgaca atagtaaatt taaccgtgta gattatgagt cgtcagaagc
     1021 ctatacgcaa gcaatacaaa actgggatgc cggtaaagta ttctataaag caattagcaa
     1081 ataccagggg gttaacgatt cggccaaata tttttttcag ttgcctgcta tagacttgaa
     1141 tgtttatgag caaaaaataa aacagcgttt acatttaaaa gacaccttag tggacaggag
     1201 tggcgaaagc gaaaacattg ctacaataat ttataaaaac aaagcctgcc gctactttat
     1261 aaatcatgtt tacttgcgca ttaaccgcta tgccgataag aaactgattg aggcaaaaca
     1321 tattgttatc gaaatatatt atggttgcta atgttacgca agtaacaaac cattgcgcat
     1381 taacgaagtg tactaaaata aattgggtgt ttggttacgc gcaagccatt ttataaatta
     1441 tgcaacttaa accacccaca atttttttta cattttatgt tgcttgtaat gtgccacgct
     1501 aaagggtgtt tttttagcaa cttcaatagc ctcaataaaa accgtttcaa cttggcgcat
     1561 cagcttaggt gtaagtttgg ttaccgttat cccttttacc atgctacggt ttttcgaaag
     1621 cagtattttc gggtatagtt gaatgtcaaa accacgcata aaacatactt ctacagcatc
     1681 gggttttata ggctttaaat aacaaaccca tgtagtatga tagtaaaacg gaatattgaa
     1741 ttttacttta cctgatattt gcggatagct aacaaacata ttgtgtaacg cccacattat
     1801 tttttgttgt ggcttgttat ccaaatcgtt tataaaacta attacacc
//
LOCUS       DAOMAA010000437         1847 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_66099_length_1847_cov_2.774554, whole genome shotgun sequence.
ACCESSION   DAOMAA010000437 DAOMAA010000000
VERSION     DAOMAA010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66099_length_1847_cov_2.774554"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PLO59_06395"
     CDS             <1..186
                     /locus_tag="PLO59_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00771.1"
                     /translation="QKNKNIETGRWPKATSKINRQYLTCTGTTTSPTDSIALDSLLTL
                     PFTDSLHLQDLQTDSIK"
     gene            313..>1847
                     /locus_tag="PLO59_06400"
     CDS             313..>1847
                     /locus_tag="PLO59_06400"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011585904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HQV00772.1"
                     /translation="MNSVKYIFVTGGVTSSLGKGIISASLAKLLQARGYSVSIQKLDP
                     YINVDPGTLNPYEHGECFVTNDGAETDLDLGHYERFLNVPTSQANNVTTGKIYQTVIN
                     KEREGAFLGKTVQIIPHITDEIKRRIKLLGETGAYDIVITEIGGTVGDIESLPFIESV
                     RQLNWEMQSSTCVIHLTLLPFLNTTGELKTKPTQHSVKALLEYGIQPDILVCRSEKHI
                     PSDMRRKIALFCNVNVNAVIESIDATTIYDVPILMMKEQLDKVVLSKLKLPLKQEPDL
                     ATWKTFLGKYKNPVTEVNIGLIGKYVELKDAYKSINEALIHAGVNNETKVNIHWIHSE
                     KLDVNNVAEKLGNLHAILVAPGFGSRGIDGKIEAVAFARENKIPFLGICLGMQFAVVE
                     FARNVMKLKDANSTEMQANTPYPVIDILADQKKITKMGGTMRLGEYACMLAKGSKIAS
                     IYHNKTKIMERHRHRYEFNNKFLKRFEEAGMMATGINPEGELVEVVELKNHPFFIGVQ
                     YHP"
BASE COUNT          648 a          343 c          360 g          496 t
ORIGIN      
        1 caaaaaaata aaaacataga aacaggtcga tggccaaaag ccacatcaaa aattaacaga
       61 caatatctta cctgtacagg cactacaact tcacccactg atagcattgc cttagactcg
      121 ctgttaacct taccatttac cgatagttta catctgcaag acttacaaac tgatagtata
      181 aaataaccct aacaatgtct tttataattt aaaaacaagg cgcttaaact tattagctaa
      241 aataaaagtg gcagaggcga ctaataaaat tacttttgca tcccgtgttc aaacttttaa
      301 cacaacaaac ttatgaactc ggttaaatac atttttgtta ccggtggggt aacatcatca
      361 ttaggcaaag gcattatatc ggcatcactg gcaaaactgc tacaggcacg cggttatagt
      421 gtttccattc aaaaactgga tccatacatt aacgttgatc cgggcacatt aaacccatac
      481 gaacatggcg agtgttttgt aaccaacgat ggagccgaaa ccgatttaga tttgggtcat
      541 tacgagcgat ttttaaatgt gccaacttcg caagccaaca atgtaaccac gggtaaaata
      601 taccaaaccg taattaacaa agaacgcgaa ggtgcttttt tgggtaaaac cgttcaaata
      661 attccgcata taactgatga gataaaacgc aggataaagt tattaggcga aacgggtgct
      721 tatgatattg ttatcactga aattggtggt accgttggtg atattgaatc gctgccgttt
      781 attgaaagtg tgcgtcagct aaactgggag atgcaatcaa gcacctgtgt aattcacctt
      841 acattgttgc cctttttaaa tacaacgggc gagttaaaaa ccaagcccac acaacattcg
      901 gtaaaagcac ttttagagta tggcatacag cctgatatat tggtttgccg cagcgaaaaa
      961 catataccat cagatatgcg caggaaaatt gcactgttct gtaatgttaa cgtaaatgcc
     1021 gttatcgaaa gtattgatgc aactactatt tacgatgtgc ccattttaat gatgaaggag
     1081 cagttggata aagtagtatt aagcaaatta aaattaccat taaaacaaga acccgactta
     1141 gctacctgga aaacattttt aggtaaatat aaaaacccgg ttaccgaagt taatattggt
     1201 ttgattggaa agtatgtaga gttaaaagac gcctacaaat caataaacga agcattgata
     1261 catgccggtg taaacaatga aaccaaagtg aatatacatt ggatacattc agaaaaactg
     1321 gatgtaaata atgtggccga aaagttagga aacctgcatg ctatattagt ggcacctgga
     1381 tttggcagcc gtggtattga cggtaaaatt gaagcggttg catttgcacg cgaaaacaaa
     1441 attccgtttt taggtatttg cttaggtatg cagtttgccg ttgttgaatt tgcacgtaat
     1501 gtgatgaaac ttaaagatgc caacagcaca gagatgcagg caaacacacc ctaccccgtt
     1561 attgatatac tggccgatca gaaaaaaatc accaaaatgg gcggtaccat gcgcttaggc
     1621 gaatatgctt gtatgcttgc aaaaggaagt aagatagcca gcatatatca taacaaaacc
     1681 aaaattatgg aacgacacag gcatcgttat gagttcaaca ataaattttt aaaacgattt
     1741 gaagaagcag gtatgatggc caccggtatt aatcccgaag gcgaacttgt tgaagttgtt
     1801 gaacttaaaa atcatccatt ctttattggt gtgcaatatc atcccga
//
LOCUS       DAOMAA010000438         1847 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_66127_length_1847_cov_1.957031, whole genome shotgun sequence.
ACCESSION   DAOMAA010000438 DAOMAA010000000
VERSION     DAOMAA010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66127_length_1847_cov_1.957031"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="PLO59_06405"
     CDS             <1..235
                     /locus_tag="PLO59_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00773.1"
                     /translation="PVKDLAQISFSITDDVNIQFTVLNEIGKAVMILPSKPYRAGQHQ
                     ATFDATTLKDGFYFLQISGTNLLEAKPFSVIRH"
     gene            complement(491..1375)
                     /locus_tag="PLO59_06410"
     CDS             complement(491..1375)
                     /locus_tag="PLO59_06410"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HQV00774.1"
                     /translation="MLPKSKIEKILIPYDFSETAALALEHGVFMAKLLRAEIKLVHVL
                     EAMSFTSAISHAFSGFEKKIETASDDKLNEVAEKIHNESGVTVSIATEVGRIYKRIAH
                     TAKVWHADLIIMGTHGVSGPQEHVVGTNTVRVIAEAPCPVISVQTHARKVGFTRILVP
                     IDDSANSRQKVPYVLTLADAYKAHVFILAFNPNTSKEDVKRKFEIKVEQTETFVQDYD
                     IITQVVYSDDKNVGEATLKFANQNDIDLILIMTEQEFSITGTSLGAFANHIINHSKTP
                     VLSCGPREVNANTMSVGY"
     gene            complement(1446..>1847)
                     /locus_tag="PLO59_06415"
     CDS             complement(1446..>1847)
                     /locus_tag="PLO59_06415"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HQV00775.1"
                     /translation="LDLSIESRDKVNKAIEIAKYFGSQIHIISLFDTDDEFLKSKLTL
                     QMHSVQQFVLDEGVEVSAKLVSSKDASDTVIAYAQNIDADLIVIIAQSDSEFSGWFLG
                     TEAHDIINNSPIPVLSVRANDRKDLYEYVLS"
BASE COUNT          587 a          346 c          297 g          617 t
ORIGIN      
        1 ccctgttaag gatttggcac aaataagttt ttcaataact gatgatgtta atattcaatt
       61 tacagtgttg aatgaaattg gtaaagccgt tatgattctg ccatcaaaac catatcgtgc
      121 cggccagcac caggcaacat ttgatgctac aactttaaaa gatggctttt actttttaca
      181 aatatcaggt acgaacttgt tagaagcgaa accatttagc gttatccgtc actaaatact
      241 ttttatactt aaaataaaag ccattgtaaa ttaaattttg caatggcttt ttaattaata
      301 atcccaatta tagatcaaaa ctaatagaac catatcattt gccaaagcag atacagttgt
      361 ttcaagcaat tgcatacttt ataaatatta tttaattgag ctgatgtaat tattgactat
      421 tactaaaatg ataaaaaaaa atccattaca aaactgcaat ggatttaaat tttaaacaaa
      481 atgtacaata ttaataaccc acactcatgg tattggcatt aacctcgcgt gggccacaag
      541 aaagtaccgg agttttcgaa tggttaataa tatgatttgc aaatgcacct aacgaagtac
      601 cggtaatact aaactcttgt tccgtcatta tcaaaattaa atcaatatcg ttttgatttg
      661 caaatttaag cgtggcttcg cctacatttt tatcatcgct atacactact tgagtaatga
      721 tgtcatagtc ttgcacaaag gtttcggttt gctcaacttt aatttcaaat tttcttttta
      781 cgtcttcctt tgaagtattt gggttaaacg ctaagatgaa aacatgtgct ttatatgcat
      841 cggctaatgt taatacataa ggcaccttct ggcgcgagtt tgccgaatcg tcaataggta
      901 ctaaaatgcg tgtaaaccca accttacgcg catgtgtttg taccgatatt accggacaag
      961 gagcttcggc aattacgcgt actgtattgg taccaactac atgttcttgt gggccactta
     1021 cgccatgggt acccataatt attaaatcgg catgccaaac ctttgctgta tgcgcaatcc
     1081 ttttatagat acggcctacc tctgtagcaa tacttacagt aacccctgat tcgttgtgaa
     1141 ttttttcggc tacttcgttt aacttatcat ccgatgcggt ttcaattttc ttttcaaaac
     1201 cactaaatgc atggctgatt gccgaagtaa acgacattgc ctccaataca tgaaccagtt
     1261 taatctctgc acgtaataac ttagccataa aaacaccgtg ttccaatgct aaagcagcag
     1321 tttcgctgaa gtcgtatgga ataagaattt tttcaatttt tgattttggt agcatagctt
     1381 ctctctttta tatactgcta aaatattgta tttatttatt aaacaaaaaa tcaactaaaa
     1441 ctattttagc taagcacgta ttcatatagg tctttgcggt cgttagctct tactgataat
     1501 accggtatgg ggctgttatt aattatatca tgtgcctctg tacctaaaaa ccagcccgaa
     1561 aattcagaat ccgactgggc aattatcaca attaaatcgg catcaatatt ttgtgcgtaa
     1621 gcaattacgg tatctgatgc atctttactt gatacgagtt tagcagaaac ttcaacgcct
     1681 tcatctaata caaattgttg tacggaatgc atttgtaatg ttagcttgct ttttaaaaac
     1741 tcatcatcgg tatcaaacaa agaaatgatg tgaatttgag aaccaaagta tttagcaatt
     1801 tcaattgctt tatttacctt atcacgcgat tcgatgctta agtcgag
//
LOCUS       DAOMAA010000439         1845 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_66237_length_1845_cov_3.130168, whole genome shotgun sequence.
ACCESSION   DAOMAA010000439 DAOMAA010000000
VERSION     DAOMAA010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66237_length_1845_cov_3.130168"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1845
                     /locus_tag="PLO59_06420"
     CDS             <1..>1845
                     /locus_tag="PLO59_06420"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HQV00776.1"
                     /translation="PSMAFFHADSTTYVPTWNGYNPTLANVGAPGSPTGGVELKSQCI
                     SGVEFYMGDKFPAFYKGQLFVAEYDTGYICNIKLDSANNPLFVDTFATSLGDCMALTY
                     NRFDQAIYYIEYPYEIHRIRYTLGVNVAPVARIQQDKLFGPGTLTVNFNGSTSTDPEG
                     AALTYAWTFGDGTTATGAQVSHTYNAPNGNPIKYDVTLTVTDTGNLSGSATSLVSLNN
                     TPPNVAITSPIDGSWYSTLVAETFNLTANVTDAEHNQNQLHYEWYTSLHHNYHEHPNP
                     IDTDHVSYFITSPEDSLETYFYEITLTVTDDAGLSNKHTVFIHQGNSPIAEFAMDKTT
                     ICLFDSVQYTSVIIGKADSLKWIFQNGTPPTSNQPNPVVTYKTMFGVQDVKLIAYNPF
                     GNDTANYLDVLKVKAAPNPQQNITGTLFSCVDDSVALHTGYDPTYTYQWYKNLQPIIG
                     ATDTHYLATNTGTYQVFVSQPNGCSTGTQPLYFAEHIINNQLTTSGSLMLCTQDSVIL
                     YADQNPDYTYQWFLNNVPIANSNYYTLTVNKTGNYKAFITDLNLCFDTSQTVTVTKVP
                     ESEAYYVSDSVLCPAGDTVMLLANTGSALSYQWYNFDVPINAATNISY"
BASE COUNT          567 a          411 c          343 g          524 t
ORIGIN      
        1 ggccatctat ggcatttttt catgccgact caaccactta tgtgccaacc tggaatgggt
       61 acaatcctac attagccaat gttggtgcac ccggttcgcc taccggtggc gttgagctta
      121 aaagccaatg tatttcgggt gttgagtttt atatgggcga taagtttccg gccttttaca
      181 aaggtcagct ttttgttgcc gaatacgata ccggttatat ctgtaatata aagttagata
      241 gtgccaataa ccccttgttt gttgatactt ttgctacaag tttaggcgat tgcatggcat
      301 taacttacaa ccgtttcgac caggcaattt attatattga atatccttac gaaatacacc
      361 gcatcagata caccctgggc gtaaatgttg cacctgtagc acgtatccaa caagacaaac
      421 tttttggccc gggcacgctt accgtaaact ttaacggcag tacatcaacc gaccccgaag
      481 gcgctgcact aacttatgct tggacttttg gtgatggcac tacagctaca ggcgcgcaag
      541 taagtcatac atacaatgcg cctaacggaa accctattaa atatgatgta acacttaccg
      601 taactgacac aggcaacctt tcgggtagtg caaccagctt ggtgtcatta aacaacacac
      661 cgcccaatgt ggctattaca agccccattg atggtagttg gtactctaca ttagtggccg
      721 aaacttttaa ccttactgca aatgttaccg atgccgagca taatcaaaat caattgcatt
      781 atgaatggta cacttcattg caccacaatt accatgagca tccaaatcca attgataccg
      841 accatgtaag ttacttcata acctcgcccg aagacagttt agaaacttac ttctatgaaa
      901 taaccttaac cgtaactgac gatgcagggc tatcgaacaa acacactgtt ttcattcatc
      961 aaggaaactc gccaatagcc gaatttgcta tggataaaac aaccatctgc ttatttgatt
     1021 cggttcaata caccagcgta atcattggca aagctgatag tttaaaatgg atatttcaga
     1081 atgggacacc gccaacctca aaccaaccaa atccggttgt aacttataaa accatgtttg
     1141 gtgtgcagga tgttaaactg attgcttaca accctttcgg aaacgatact gcaaattact
     1201 tagatgtgct caaagtaaag gcagcaccca acccacaaca aaatataacc ggtactttat
     1261 ttagctgtgt tgatgatagt gtggcattgc atacaggtta tgacccaaca tacacgtatc
     1321 aatggtataa aaacttgcaa ccgataatag gcgcaaccga cacacattat ttagcaacaa
     1381 ataccggcac ttatcaggta tttgtgtccc agccaaatgg atgtagtacc ggtacccaac
     1441 cactttattt tgccgaacat attataaata accaattaac aacaagtggt tcgttaatgc
     1501 tatgtacgca ggatagcgtt atactttatg ccgatcaaaa tccggattat acctatcaat
     1561 ggtttttaaa taacgtgcct attgcaaata gcaactacta cacacttact gtcaataaaa
     1621 ctgggaatta taaagcgttt attaccgatt taaatttgtg ttttgatacc agtcaaacgg
     1681 ttacggtaac taaagtgccc gaatcggaag cttattatgt ttctgatagt gtgctttgcc
     1741 cggcaggcga taccgtaatg ttattggcaa atacaggcag tgcactttca tatcaatggt
     1801 acaattttga cgtgcctatt aatgctgcta caaatatttc gtatg
//
LOCUS       DAOMAA010000440         1840 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_66604_length_1840_cov_2.954062, whole genome shotgun sequence.
ACCESSION   DAOMAA010000440 DAOMAA010000000
VERSION     DAOMAA010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66604_length_1840_cov_2.954062"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1265)
                     /locus_tag="PLO59_06425"
     CDS             complement(<1..1265)
                     /locus_tag="PLO59_06425"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HQV00777.1"
                     /translation="MRILLLIVFLITGNCSLAQVLSTTRTTNWKKAKHIGAYFEPSLT
                     VNILQYGGNNNGAIANDAALIAAVNALNGMPGIIYLPAGNYLFNNTINLRDSLILRGS
                     GTNTVLTFSFNNASKHCITISGSVMGGWVSVGSGLRKNSKWIKPAVITSFKVGDLLEM
                     RQLNGSWDTQPVAWAQYAVGQILYVDSIKNDKLYFHEKLRIDYDTLLQPQVARLKPRK
                     SVGVECLKITRTDSNAAGNNYGVFLNKAYNCWIKNVTIEKMISAHVCITTSARCEITG
                     SYITDAYSFDGSNTHGYGVLINEHACENKIENNVFKKLRHAITCKQGANGNVIAYNYV
                     TQPTRTEFPTDAGADLLLHGHYPFLNLFEGNICQNIQADQTWGPNGPYNTFMRNRADG
                     YGLIITSGSISTNNQNIIANDITNTGFLKG"
     gene            1573..>1840
                     /locus_tag="PLO59_06430"
     CDS             1573..>1840
                     /locus_tag="PLO59_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00778.1"
                     /translation="MASCTNKNYNTYTYNADSIQTNKYSPPEIIFIDSCAPPADALIE
                     KTAHNPTSLNPPEIKPASFYLTMPVYNNDNGLPLNAILCSFCDAK"
BASE COUNT          576 a          365 c          311 g          588 t
ORIGIN      
        1 ccttttaaaa aaccggtgtt agtaatatcg ttggctatta tgttttgatt gttggtagat
       61 atactgcccg atgtaataat taatccataa ccatcggcac ggttgcgcat aaaggtatta
      121 taagggccat taggacccca ggtttgatcg gcctgtatgt tttggcagat attaccttca
      181 aataaattta aaaacggata atggccatgc aacaacaaat cggcaccggc atcggtcgga
      241 aactcagtac gagttggttg cgtaacataa ttataagcaa taacatttcc atttgcgcct
      301 tgtttgcatg taatggcatg ccgcagtttt ttaaacacat tgttttcaat tttattttcg
      361 catgcatgtt cgttaattaa cacaccgtag ccatgtgtgt tacttccatc aaaactataa
      421 gcatcggtta tataacttcc ggtaatttca catcgggccg atgtagtaat acaaacatgg
      481 gcgcttatca ttttttcgat ggttacattc ttaatccaac agttataggc tttgtttaaa
      541 aaaacaccgt aattgttacc ggcagcattg ctatcggtac gagtaatctt taaacattca
      601 acacctacac tttttctggg ctttaaacgt gctacttgcg gttgtagaag tgtatcataa
      661 tcaatccgca gtttttcatg aaaatatagt ttatcatttt tgatactatc cacatataaa
      721 atttgcccca cagcatactg tgcccatgcc acaggttggg tatcccaact accatttagt
      781 tgccgcatct caagcaaatc gcccacttta aaacttgtta taacagcagg tttaatccac
      841 ttactgtttt tgcgtaagcc actgcctaca cttacccacc cgcccattac tgaaccgctt
      901 atagttatac aatgttttga agcgttgtta aaactaaaag ttagtacggt attagttcca
      961 ctgccacgta atattaaact atcgcgtaaa ttaatggtgt tgttaaacag ataattgccg
     1021 gcaggcaaat atattatgcc gggcatgcca ttaagcgcat taactgctgc aataagtgca
     1081 gcatcattag caatggcacc attgttatta ccaccatatt gcagtatatt aactgtaagt
     1141 gatggctcga agtaagcgcc tatatgtttt gcttttttcc agtttgtggt gcgtgtagta
     1201 cttaacactt gtgctaagct acaattaccg gttattaaaa aaactatcaa tagtaaaatg
     1261 cgcatgtgtt atggttttgg tttttaaata agtaaaaaca gatgtataat ttgatagttg
     1321 aaatatataa actatttact atggtaagga catcagaatg tttccattta aaaaattaaa
     1381 tttagttgca ttgataaggg tgccgataaa caacaagact tttttgtgca acacggctaa
     1441 ctttaagtca tagtgattaa cttcgttgta acacagccat gccatcctat gcttttacac
     1501 attattacgc tgcctagcaa aaaagctaaa aacattaata ggttttatta tctcattgca
     1561 gtgctgttgc ttttggcatc atgcacaaac aaaaactata atacctacac ctacaatgcc
     1621 gattcaatac aaacaaataa atacagccca cccgaaatta tttttattga tagctgtgcg
     1681 ccaccagcgg atgcattaat tgaaaaaaca gcgcataatc ctacatcttt aaatccacca
     1741 gaaataaaac ctgccagttt ttatctgaca atgcccgttt acaataacga taacggatta
     1801 ccacttaacg caatactttg cagcttttgt gatgcaaaag
//
LOCUS       DAOMAA010000441         1835 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_66979_length_1835_cov_2.292697, whole genome shotgun sequence.
ACCESSION   DAOMAA010000441 DAOMAA010000000
VERSION     DAOMAA010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66979_length_1835_cov_2.292697"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="PLO59_06435"
     CDS             complement(<1..669)
                     /locus_tag="PLO59_06435"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HQV00779.1"
                     /translation="MSVNNFQYIIIGGSTKCATTSVFAYLANHPSIASSKVKESRFFW
                     MDEYPLKNGAYNIVDGIESYEQVFLEKTGANIRLEATPDYLYSRDTALAIKENLEDVK
                     LIFIVRNPIERIISWYKFAKQLNLILATETIEAYINQQLNYVGNDAPQHLRSVEQGLY
                     GKYLKHYFEIFGKDKICVLDYEQISSNPLKAMKSIADFLKIDAAFYESYSFEVLNKSV
                     QVKDA"
     gene            complement(700..>1835)
                     /locus_tag="PLO59_06440"
     CDS             complement(700..>1835)
                     /locus_tag="PLO59_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014782330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQV00780.1"
                     /translation="LLSEEHKLIRNTVREWVKKEVSPIIEDYAQRAAFPKHLISGLAG
                     IGAFGPYIPVEYGGAGLDQISYGLIMQEIERGDSGLRSTASVQSSLVMYPIYTYGSEA
                     QRKKYLPKLATGEWLGCFGLTEPDAGSNPGGMLTNYKDAGDHVILNGSKMWISNAPFA
                     DIAVVWAKDEAGDIRGLVVERGMEGFTTPETHGKWSLRASATGELVFDNVKVPKENIF
                     PEIKGLKGPLGCLNSARYGISWGVIGAAMDCYDSALRYAKERKQFGRPIAGFQLQQKK
                     LAEMVTEITKAQLLCWRLGVLKNEHPATPQQISMAKRNNVNMALEIAREARQIHGGMG
                     ITGEYPIMRHMMNLESVVTYEGTHDIHLLITGMHITGENAFGG"
BASE COUNT          516 a          426 c          306 g          587 t
ORIGIN      
        1 tgcatcctta acctgaactg acttattaag cacctcgaaa ctataagatt cataaaacgc
       61 tgcgtcaatt tttaaaaaat ctgctataga tttcatggct tttaatggat tagacgaaat
      121 ttgctcataa tcgagcacac aaattttatc ctttccaaat atttcgaaat agtgttttag
      181 atacttaccg taaagcccct gttcaacaga tcgtaagtgt tgtggcgcat catttccaac
      241 atagtttaat tgttgattga tataagcttc aatggtttca gttgccaaaa taagattaag
      301 ttgctttgca aatttatacc aactgataat cctttcgatt gggttcctaa caataaaaat
      361 caatttaaca tcctccaggt tttcttttat cgcaagcgct gtatctctgc tatacaaata
      421 atccggtgtt gcttctaaac gaatattggc tccggttttt tctaaaaaaa cctgctcgta
      481 tgattcaatc ccatctacaa tattgtaggc cccattttta agcggatatt catccatcca
      541 aaaaaacctg ctttccttaa ccttactcga tgcaattgac gggtggtttg ctaaataagc
      601 aaacaccgaa gtggtggcac acttagtact accaccaata attatatatt ggaaattgtt
      661 aactgacatg aaaaattaaa aaatgttcta tgctaaatac taacccccaa aggcattttc
      721 accggtaatg tgcatacctg ttattagcaa gtgtatatcg tgtgtgcctt cataagttac
      781 aaccgattct aaattcatca tgtgtcgcat aattggatat tcgcctgtaa tgcccattcc
      841 tccatgtatt tgtcgtgctt cgcgagcaat ttctaaagcc atgtttacat tattacgttt
      901 agccatgctg atttgttggg gtgtagcagg gtgctcgttt tttaaaacac ctaatcgcca
      961 gcaaagaagc tgtgctttgg taatttctgt taccatttcg gctaatttct tttgctgtaa
     1021 ttgaaatccg gcaatgggtc gtccaaactg cttacgttct tttgcatatc gcaaagccga
     1081 gtcataacaa tccatagctg caccaattac gccccatgat attccataac gcgcactatt
     1141 taagcagccc agcggacctt taagcccctt tatttcaggg aaaatatttt ccttgggcac
     1201 ttttacatta tcaaatacca actcgcctgt tgcgctggca cgtaaactcc atttgccatg
     1261 cgtttcaggt gttgtaaagc cttccatgcc acgttctaca accaaacctc ttatatcacc
     1321 cgcttcgtct ttagcccaaa ccaccgctat atcagcaaag ggtgcattac ttatccacat
     1381 tttgctaccg ttcagaatta catggtctcc tgcatcttta tagtttgtta gcattccgcc
     1441 cggattacta ccagcatccg gttcagttaa accaaaacag cccaaccact cgcccgttgc
     1501 cagctttggt aagtactttt tacgttgggc ctcactgcca taagtataaa tcggatacat
     1561 taccaaagaa ctttgtacgc ttgctgtact acgtaaaccc gaatcgcctc gttcgatttc
     1621 ttgcattatc aatccataac taatttgatc taatccagcg ccaccatact caacgggtat
     1681 gtaagggcca aatgcaccaa tgccggctaa accactaatt aaatgtttgg gaaaggctgc
     1741 acgctgggca taatcttcta tgatgggcga aacctctttc tttacccatt cgcgtacggt
     1801 gtttctgata agtttatgct cttccgatag taaat
//
LOCUS       DAOMAA010000442         1832 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_67133_length_1832_cov_3.186269, whole genome shotgun sequence.
ACCESSION   DAOMAA010000442 DAOMAA010000000
VERSION     DAOMAA010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67133_length_1832_cov_3.186269"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="PLO59_06445"
     CDS             <1..876
                     /locus_tag="PLO59_06445"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HQV00781.1"
                     /translation="NLQELLNGIKEFVDTGENGIPAPMLESDDETVTTPELTFSTELN
                     FDASPETDENNTSIRTIDLFMQDIALLTDADKNKDDGNTDVVSLMTIHASKGLEFKNV
                     FVVGLEENLFPSMMAMQSRADLEEERRLFYVALTRAEKKLFLSFAVSRYKFGNLMGCE
                     PSRFLDEIDPAYTEVTYHKAQRSETISPGLSDFRKASSNLRPPTKPLLNNVSSSQKPP
                     AHFKKVSTTGNQTFIGDDTNQIVAGMQVEHERFGFGKVLAIEGKFPETKCTIFFKNTG
                     NKQLLLKYAKLKIVG"
     gene            879..>1832
                     /locus_tag="PLO59_06450"
     CDS             879..>1832
                     /locus_tag="PLO59_06450"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HQV00782.1"
                     /translation="MILPSDFVNHLKQHINQADKVLDALTQPSNTSIRHNVSKTKNAF
                     DLKHLEQIAWAENGYYLSQRPLFTVDPFFQSGYYYVQEAGSMFIGEIIKQLGLNKSTV
                     CGLDLCAAPGGKSTHVLSMLQPESVLVSNEIIKTRTPVLIDNLIKWGLPNYIVTQSDA
                     RKFGLLPALFDFILCDAPCSGEGMLRKSNDALHHWSLNNVNLCAQRQQRIVHDVWPSL
                     KSGGYFIYATCTFNVIENEENITSFTETLPANSIKLQTQNGIVESITKNKNGHLVYGY
                     RLMPGLVNAEGLFIAVFQKSEAVPNNLSRIKSPAIKTGHYKN"
BASE COUNT          627 a          349 c          350 g          506 t
ORIGIN      
        1 aacctgcagg agttgcttaa tggtattaaa gagtttgttg atacaggcga aaacggcata
       61 ccggccccca tgctcgaaag cgatgatgaa actgttacaa caccggaact aacttttagt
      121 acggaattaa attttgatgc atcacctgaa acagatgaaa ataatacgtc aataagaact
      181 attgatttgt ttatgcaaga tattgcatta cttaccgatg ccgataaaaa taaagatgat
      241 ggaaatacag atgtagtttc gttaatgacc atacatgctt cgaaaggact ggagtttaaa
      301 aacgtatttg ttgtagggtt agaagaaaac ctgtttccaa gcatgatggc catgcaaagc
      361 cgtgccgacc ttgaagaaga aaggcgcctg ttttatgtgg cattaacccg tgccgaaaaa
      421 aaattatttc tgtcctttgc tgtatcacgc tataagtttg gcaatttaat gggctgcgaa
      481 cccagcaggt ttttagatga aattgacccg gcctatacag aagttactta tcataaagca
      541 caaagatcag aaacaatatc accaggtttg tctgatttta gaaaagccag ctccaattta
      601 aggccaccca ctaaaccatt acttaacaat gtaagcagtt cgcaaaagcc acctgcacat
      661 tttaaaaaag tatctacaac cggcaaccaa acttttattg gcgatgatac caaccaaata
      721 gttgccggta tgcaagtgga acatgagcgt tttggctttg gaaaagtact tgccattgaa
      781 ggaaaatttc cggaaaccaa gtgcaccata ttttttaaaa acacaggcaa caaacaatta
      841 ctacttaagt atgcaaagct taaaattgta ggctgaaaat gattttacca tccgactttg
      901 taaatcattt aaaacaacat attaaccaag ccgacaaagt attggacgcc ttaacgcagc
      961 catccaatac gtcaatcaga cataatgttt cgaaaacaaa aaatgctttc gaccttaaac
     1021 atttagaaca aattgcctgg gccgaaaatg gctattattt atcgcaacgc ccgttgttta
     1081 cagtagatcc gttttttcag agtggatact actatgtaca agaggccggc agtatgttta
     1141 ttggtgagat tattaaacaa ttaggcttaa ataaaagtac ggtttgcggc ttagatttat
     1201 gtgctgcacc cggtggtaaa agcacccatg tactcagcat gcttcaaccc gaaagtgttt
     1261 tggtgtcaaa cgaaattatt aaaacgcgca cacctgtatt aattgataat ttaataaaat
     1321 ggggcttgcc taattatatt gttacgcaaa gcgatgcgcg caaattcgga ttgctaccgg
     1381 cactttttga ttttatatta tgcgatgcac cgtgcagtgg cgaaggtatg ctcagaaaaa
     1441 gtaatgatgc actacatcat tggagtttaa acaatgtaaa cctttgtgca caaaggcaac
     1501 agcgcattgt acatgatgtt tggccaagct taaaaagtgg cggctacttt atatatgcaa
     1561 cctgtacttt taatgttatc gaaaacgaag aaaatataac atcatttacc gaaaccttgc
     1621 ctgcaaacag tattaaactt caaacccaaa atggcatagt tgaaagtatt accaaaaata
     1681 aaaacggcca tttagtatat ggctaccgtt tgatgcccgg tttggtaaat gccgaaggtc
     1741 tatttattgc cgtatttcaa aaaagtgaag cagtgccaaa caatttaagc cgcattaaat
     1801 cgcctgcaat taaaaccggc cattacaaaa at
//
LOCUS       DAOMAA010000443         1831 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_67199_length_1831_cov_3.173986, whole genome shotgun sequence.
ACCESSION   DAOMAA010000443 DAOMAA010000000
VERSION     DAOMAA010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67199_length_1831_cov_3.173986"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..436)
                     /locus_tag="PLO59_06455"
     CDS             complement(80..436)
                     /locus_tag="PLO59_06455"
                     /inference="COORDINATES: protein motif:HMM:NF024489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00783.1"
                     /translation="MSILKKENQNLFVPRDISWLSFNHRVLQEAADKQVPLIERIKFL
                     GIFSSNLDEFFRVRVATIRRLIKTRSKGKDFFNYSAEEILSQIQLIVKSQQEKFEEIY
                     KGILQELEDNNIFIIN"
     gene            complement(438..944)
                     /locus_tag="PLO59_06460"
     CDS             complement(438..944)
                     /locus_tag="PLO59_06460"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HQV00784.1"
                     /translation="MKSLYLLRHAKATDKLATVDALRPLSTNGIIDANLMFDAIKTKL
                     QKPDLIITSPALRAHHTALIFANCLSIDLNKIHTEAGLYLYHLQYLIDTIVQLPNEIN
                     VALLVGHNPHFEQLGTLLIPDLEKLPTSGLLEFKLENGWENLNQKSVLSYKMYLAAAF
                     RNNQFKII"
     gene            1135..1830
                     /locus_tag="PLO59_06465"
     CDS             1135..1830
                     /locus_tag="PLO59_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00785.1"
                     /translation="MYRTLLFIVAFTGFGLANAQMIVAPIKQKYWASSSELIFSFGNV
                     EDPNLDVNNVLRFSCFLHVQEQLHYNFTNHVGVFTGLSLRNVGFINDLNDTVKLKQRV
                     YTLGLPVGIKLGNMSSSTYATIGVEGELAFHYKQKVFVNDEKSRTSIWFSNRTNTFLP
                     SAFVDINFKNGAYVRFKYYLDDFLIGKNQKINVAGVSFNPTQSTLFYISLGLVLPSYK
                     SNKINIPGTNNNL"
BASE COUNT          574 a          275 c          320 g          662 t
ORIGIN      
        1 ttaccggtgt taaaagaggc agtacatttt ctctgaagta tgaacggata taattgcctt
       61 gggtaagcga aagttgcttt taattaatta taaaaatgtt gttatcctca agttcttgta
      121 atatgccttt atatatttct tcaaacttct cttgttgcga ttttacaatg agttgtattt
      181 gactgagaat ttcttcggct gagtagttaa aaaaatcctt gcctttactt cttgttttta
      241 ttaatcgcct tatagttgcc acccttactc tgaaaaattc atcaaggttg ctcgaaaaga
      301 ttcctaaaaa ttttatccgt tcaattagtg gaacttgttt gtcggctgct tcttgtaata
      361 cgcgatggtt aaaacttaac caactgatat cacgaggtac aaataaattt tgattttctt
      421 ttttaagaat tgacacttta aattattttg aattggttat tacgaaacgc agctgctaaa
      481 tacatcttat agctcaaaac agacttttga tttagatttt cccatccgtt ttcaagttta
      541 aattcgagta agcctgatgt tggcagtttt tctaaatcgg gtattaacaa tgtgcctaac
      601 tgctcgaaat gtggattatg accaaccaac aatgctacat taatttcgtt aggcaattga
      661 actatagtat caattaaata ctgtaagtga tataagtaca ggccggcttc ggtatgaatt
      721 ttatttaaat caattgataa acaattagca aaaattaatg cagtgtgatg tgcgcgtaat
      781 gccgggcttg ttattattaa atcgggtttt tgaagctttg ttttaatagc gtcaaacata
      841 aggttggcat caataatacc attggtactc aaagggcgta gtgcatcaac tgttgcaagt
      901 ttatcagttg ctttggcatg tctgagtaag taaaggcttt tcacagaagg actataattt
      961 aagtgtttca aaattatttt tgatttttaa tttaccggag gtacatgagt tgatttaaca
     1021 atttgttaaa attgcactac tttttagcta agcttgagca gcataagaat taaaatagtt
     1081 taagttgaac caaaaggtat taaactacat ttgagaaaaa taaaaattta ttatatgtat
     1141 agaactttac tttttattgt agcatttaca ggatttggtt tggcaaatgc ccaaatgatt
     1201 gttgcgccta ttaaacaaaa atattgggct tcttcatctg aacttatttt ttcattcggc
     1261 aacgttgaag atccgaatct ggatgtaaac aatgtgcttc ggttctcatg ttttttacat
     1321 gtacaggaac agttacatta taattttacc aaccacgtag gtgtttttac aggcctttcg
     1381 cttcgtaatg ttgggtttat taatgattta aacgacactg taaaactaaa acaacgcgtt
     1441 tatacactgg gattgccggt aggtattaag ttaggcaata tgagtagttc aacctatgct
     1501 acaattggcg ttgaaggcga attggcattt cactacaaac aaaaggtgtt tgttaatgat
     1561 gaaaagtcgc gtacgtctat ttggtttagt aaccgtacca atactttttt accatctgca
     1621 tttgtagata ttaattttaa aaatggtgcc tatgtgcgtt ttaaatatta tttggacgac
     1681 tttttaattg gcaaaaacca aaaaattaac gtagcagggg tatcgtttaa tcccacacaa
     1741 agtactttgt tttatatatc attaggtttg gtattgccat catataaatc taataagata
     1801 aacatacccg gtactaacaa taacttgtaa t
//
LOCUS       DAOMAA010000444         1829 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_67347_length_1829_cov_3.405862, whole genome shotgun sequence.
ACCESSION   DAOMAA010000444 DAOMAA010000000
VERSION     DAOMAA010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67347_length_1829_cov_3.405862"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="PLO59_06470"
     CDS             <1..228
                     /locus_tag="PLO59_06470"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010420465.1"
                     /note="catalyzes the formation of prolyl-tRNA(Pro) from
                     proline and tRNA(Pro); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HQV00786.1"
                     /translation="STHYVDTWDDFVKTLDTKPGFVYAHWDGTPETEQKIKEETKATI
                     RCIPLDSKPETGTCIKTGKPSMQRVVFARAY"
     gene            complement(270..>1829)
                     /gene="uvrA"
                     /locus_tag="PLO59_06475"
     CDS             complement(270..>1829)
                     /gene="uvrA"
                     /locus_tag="PLO59_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HQV00787.1"
                     /translation="QTWFLNCQIHFDKRQWVIAQEILKEINKRIKFLTDVGLDYLTMF
                     RSAKTLSGGESQRIRLATQIGSQLVGVMYILDEPSIGLHQRDNMRLIKSLCDLRDNGN
                     SVIVVEHDKETIEAADYVIDIGPGAGINGGKVVAQGNPKTLKHADTLTAQYLNGVMEI
                     GLRDVRKGNGKTLTIKGAQGNNLKHIDVNFPLGKLIVVSGVSGSGKSTLINETLYPIL
                     NQHFYKSLQKPLTYKSVTGLKHLDKVIEIDQSPIGRTPRSNPATYTGVFTDIRMLFSE
                     MTEAKIRGYKPGWFSFNVKGGRCESCKGAGMRTIEMNFLPDVYVHCETCNGKRYNREI
                     LDVRYRGKSINDVLNMSIDQAVLFFENMPAILHKIKTIKDVGLGYITLGQQSTTLSGG
                     EAQRVKLATELSKKDTGNTFYILDEPTTGLHFEDVRILLEVLQKLVDKGNTVLVIEHN
                     MDVIKVADHIIDIGPEGGAKGGNIVCEGTPSQVAADKKSITGYYLKKEIQNTVAQPIQ
                     TKVLKRKAAKK"
BASE COUNT          537 a          402 c          302 g          588 t
ORIGIN      
        1 tcaacccatt atgttgatac ctgggacgac tttgtaaaaa cacttgatac caaacccggt
       61 tttgtttatg cgcattggga tggcacacct gaaaccgaac aaaaaataaa agaagaaacc
      121 aaagcaacaa tccgttgcat tccattagat agtaaacctg aaacaggcac ctgcattaaa
      181 acaggcaaac caagtatgca gcgtgttgta tttgcaagag cttattaata atgtaaaccg
      241 gttttacaaa aaaaagaaag cagctacaat tattttttag ctgctttcct ttttaaaact
      301 ttggtttgaa tgggctgtgc aaccgtgttt tgtatttctt ttttaagata gtatcccgtt
      361 atactttttt tatctgcagc tacttggctg ggtgtgccct cgcaaacaat gttaccacct
      421 tttgcaccac cttcgggccc aatgtcaatt atatgatcgg caacttttat aacatccata
      481 ttatgctcaa taaccaaaac tgtattgccc ttgtccacca atttctgcaa cacttccaat
      541 aaaatccgga catcttcaaa atgcaagcct gtagtaggtt catctaaaat ataaaaagta
      601 ttacctgtgt cttttttcga caattcagtt gccagtttta cccgttgtgc ttcgcctcct
      661 gataaagtag ttgattgctg ccccagggta atgtaaccga ggcctacatc tttaatggtt
      721 ttaattttat gcagtatggc aggcatgttt tcaaaaaaca aaacagcttg atcaatactc
      781 atattaagca catcgtttat tgatttgcct ctgtatctta catctaaaat ctcgcggtta
      841 tagcgcttac cgttacaggt ttcgcagtgc acataaacat cgggtaaaaa gttcatctct
      901 atggtgcgca tacctgcacc tttacaggat tcgcaacggc cacctttaac gttaaacgaa
      961 aaccaacccg gtttataacc gcgtattttt gcttcagtca tttcgctaaa caacatacgt
     1021 atatctgtaa acacaccggt ataagttgcc ggattactgc gcggggtacg gccaatagga
     1081 ctttgatcta tttcaattac tttatctaaa tgttttagtc ccgttacact tttataggtt
     1141 agcggttttt gcaacgattt ataaaaatgc tggtttaaaa ttgggtatag tgtttcattt
     1201 attaaagtgg atttgccact gccactaacg ccacttacca caatcagttt tcccaacgga
     1261 aagtttacat caatatgttt taagttattg ccttgcgcac ctttaatggt tagtgtttta
     1321 ccattaccct tccgtacatc acgtaaacca atttccataa caccgtttaa atactgagcc
     1381 gttaatgtat cggcatgttt caatgttttc ggattaccct gtgcaactac tttaccaccg
     1441 ttaataccgg ctccggggcc aatatcaata acataatcag cggcttctat cgtttcctta
     1501 tcatgctcga ccactattac cgaattgcca ttgtcgcgca aatcacatag cgattttatt
     1561 aaccgcatat tatctctttg atgaagaccg atactgggtt cgtctaaaat atacataaca
     1621 cccaccaact gtgaaccaat ttgcgttgcc aaacggatgc gttgcgactc gccaccactt
     1681 aaagtttttg cacttctaaa catggtaaga taatccaagc caacatcggt taaaaattta
     1741 atgcgtttgt ttatctcttt taaaatttcc tgtgcaataa cccattgacg cttatcgaaa
     1801 tgtatttgac aatttaaaaa ccaagtttg
//
LOCUS       DAOMAA010000445         1827 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_67478_length_1827_cov_3.509029, whole genome shotgun sequence.
ACCESSION   DAOMAA010000445 DAOMAA010000000
VERSION     DAOMAA010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67478_length_1827_cov_3.509029"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            574..1026
                     /locus_tag="PLO59_06480"
     CDS             574..1026
                     /locus_tag="PLO59_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017258159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HQV00788.1"
                     /translation="MTLLKFNNNLAKHFDARPVFNELYNELFENFGPMHKMHTVPTVN
                     ISESDDKFNIALAAPGLNKEDFNVNVQQGVLTISAEQKSEDKEGTAKFTRREYSYTKF
                     SRSFTLPETVKADNISATYLNGVLSLDIPKIESEILKNRSNPLEIKIS"
     gene            1305..>1827
                     /gene="surE"
                     /locus_tag="PLO59_06485"
     CDS             1305..>1827
                     /gene="surE"
                     /locus_tag="PLO59_06485"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008237757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HQV00789.1"
                     /translation="MNSKPTILITNDDGITAPGIRALANAMKHFGDIVIVAPDSPQSG
                     MGHAITINKPLRLDKVTLPDGLEGYQCSGTPVDCVKLAVNKILHRKPDLCVSGINHGS
                     NSSINVIYSGTMSAAMEGAIEGIPSIGFSLCNYALDADFEAAALFAQTIVWQVLQHGM
                     PNNTLLNVNIPDLK"
BASE COUNT          619 a          334 c          323 g          551 t
ORIGIN      
        1 ttatacatga ttaacactga cggaaccgac ttaaaacaaa taacttttga tagtatgttt
       61 gatgcgtttc ctatgttttc gtccgatggt aaaaaattaa ttttttcgag caacaggaat
      121 aacaacggca gtagcgaaac caatttattt attgccgatt gggttgaata atttaattgc
      181 ttttctgaag tttaccactt aatattttat aacactttag tcaacactga ttttatgact
      241 gaaccacaac ctaataaatt tatgtactgg cttaaaaaat tagggtggat cggttttcta
      301 ttttttctga ttaaaggttt aatatggttg gctgtgtttt ttggccttgc aaagtatttt
      361 agcaattagc ttaaactgtt tttaccagaa ccgagccatt ttgactgcaa aagtcaacag
      421 tagcagccat aaagtcgcaa cttgttttca taaaccgtca ttctgtttga tttaatcgca
      481 tggcatttca attgattgcc ataaatcgtc caacgtttga agagctccta accttggata
      541 aaaacaaaat taaacaatta aaaaaattat aaaatgacac ttttaaaatt caacaacaac
      601 ttagcgaagc attttgatgc ccgccccgta tttaacgaac tgtacaatga actgttcgaa
      661 aactttggtc ctatgcacaa aatgcacaca gtgcctacag taaatatcag cgaaagcgat
      721 gataagttta atattgcatt ggcagcaccg ggcttaaaca aagaagactt taatgtaaat
      781 gtgcaacaag gtgtattaac catcagtgcc gaacaaaaat cggaagacaa agaaggcact
      841 gctaaattta cacgcagaga gtattcttac accaagtttt cgcgctcatt tacattgccc
      901 gaaactgtaa aggccgataa cataagcgct acttatttaa acggagtatt aagtttagat
      961 atacctaaaa tcgaaagcga aatactaaag aaccgttcaa atccgttaga gattaaaata
     1021 agctagtgcc gtttaaactg caaggttaat aaaggtttaa agtcatatac aacggctctt
     1081 atacatagaa acttcatgat gatataaaaa aaataaggac ttttgacttc aataattttt
     1141 aactaagctt gcaacaaaca gtaagtataa cttacaatag aaataaaaga atttaggttg
     1201 gtttaggttg tcgggacagt agtggtactg tcccgctttt tttttttata aaacacgcct
     1261 tgtattgcat gaaacaaatt gccttataaa attacttttg cggcatgaat tcgaaaccaa
     1321 ctatattaat taccaatgac gatggcataa cagcacccgg cataagagcg cttgcaaacg
     1381 ccatgaaaca ttttggagat attgtaattg ttgcccccga tagtccacaa tcaggtatgg
     1441 gacatgcaat aaccataaac aaaccattac gcctcgataa agtaaccttg cccgatgggc
     1501 ttgaaggcta tcaatgttcg ggtacaccgg ttgattgtgt gaaactggcc gtaaataaaa
     1561 ttttgcatcg caaacccgac ttatgcgtat cgggtattaa tcatggctcc aactcgtcaa
     1621 taaatgtaat ctactcaggc accatgtcgg ctgcaatgga aggtgccatt gaaggtattc
     1681 cgtcaatagg tttttcatta tgcaattatg cattggatgc cgactttgaa gcagctgccc
     1741 tgtttgcaca aaccattgtt tggcaagtgt tacaacatgg catgccaaat aatactttac
     1801 ttaatgtaaa tattcccgac ttaaagt
//
LOCUS       DAOMAA010000446         1826 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_67582_length_1826_cov_2.360248, whole genome shotgun sequence.
ACCESSION   DAOMAA010000446 DAOMAA010000000
VERSION     DAOMAA010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67582_length_1826_cov_2.360248"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="PLO59_06490"
     CDS             <1..578
                     /locus_tag="PLO59_06490"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQV00790.1"
                     /translation="INQVYLQKAAKSLDRLTAIVNDLETISRMEAGAMIIDWRTFNLS
                     DLLTEVVDTHALRASEKNIKLFIAPATPKQIYVYADKERIRQVLDNLVVNSIKYGKSN
                     GNTEISISETINDCIVHVKDNGMGIDEAHIPRLFERFYRVDKHRSRAEGGTGLGLAIV
                     KHILESHQQSITVQSKLNEGTVFSFSIKRSV"
     gene            complement(579..1211)
                     /locus_tag="PLO59_06495"
     CDS             complement(579..1211)
                     /locus_tag="PLO59_06495"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HQV00791.1"
                     /translation="MFNSYINFLENALQKPLPGSDAHKQMMPQGRTLHAPDDARSYRN
                     GAVLLHLFPIDDKPYLLFIKRSDDGKVHGGQIAFPGGKFENTDENLKQTALRESYEEV
                     GLINALVLGALSNLYIPVSTFMVHPFISYSDNEQIWQPNAHEVETVIPISLTELNHNK
                     NKTIKPVIVSSGLQLQVPCYQINQEIIWGATAMITNELLAISKPFFNYSK"
     gene            complement(1213..>1826)
                     /locus_tag="PLO59_06500"
     CDS             complement(1213..>1826)
                     /locus_tag="PLO59_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016196067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQV00792.1"
                     /translation="LVGLYKPQQGVIKYNDIPGTEINFNDLRSQIGFVTQDTQLFAGT
                     IRENLLFVNPAATDAQCMDVLQKAACTSLLQRADKGLDTMIGEGGVKVSGGEKQRLSI
                     ARALLRAPDLLVFDEATSALDSITEEEISQTIRNISDGRNHITILIAHRLSTIMHADV
                     IYVLEQGQIIESGNHQRLLDEKGLYYAMWRQQIGERKPLKQTV"
BASE COUNT          559 a          364 c          334 g          569 t
ORIGIN      
        1 atatcaatca ggtttactta caaaaagcag ctaagagttt agacagactg actgcaattg
       61 tgaatgattt agaaaccata tcgcgtatgg aagccggtgc catgattatt gattggcgca
      121 catttaattt aagcgattta cttacagaag tagttgatac gcacgctttg cgtgcatcgg
      181 aaaaaaacat taaacttttt attgccccag ctacccctaa acaaatttat gtatatgcag
      241 ataaagaacg catcaggcaa gtactcgata atttggtagt aaactctatt aaatatggca
      301 agtcgaatgg aaacactgaa ataagtatca gcgaaacaat caatgactgt attgttcatg
      361 tgaaagataa cggtatgggc attgatgaag ctcacatacc gcgtttattc gaacgatttt
      421 accgtgttga taagcaccgc tctcgggccg aaggtggcac cggattagga cttgccattg
      481 taaaacacat tttagagtcg catcagcaaa gtattacagt gcaaagcaaa ttaaatgaag
      541 gcaccgtatt ttccttttct atcaaacgta gtgtttaact atttcgaata attaaaaaat
      601 ggtttactta tagcaagcaa ctcatttgtt atcatagctg ttgcgcccca aataatctcc
      661 tgattaattt gataacaagg cacttgtaat tgcaagccac tactaactat aacgggctta
      721 atggttttat ttttattgtg attaagttca gttaatgata tcggtataac tgtttcaacc
      781 tcatgcgcat ttggttgcca aatttgttcg ttatcagaat aagaaataaa aggatgtacc
      841 ataaaagtac ttaccggaat gtataagtta ctaagcgccc ctaaaactaa tgcattaatt
      901 aatccaactt cttcatacga ttcgcgcaag gctgtttgct tcagattctc atccgtattt
      961 tcaaatttac caccggggaa agcaatttgg cctccatgta ctttaccatc atcactacgt
     1021 tttataaaaa gcaaataggg tttatcatct atcggaaata aatgcagtaa aactgctccg
     1081 ttgcgatagc tgcgtgcatc atctggtgca tgtaatgtac gaccttgtgg catcatttgc
     1141 ttatgtgcat cgctaccggg taatggtttt tgcaaggcgt tttccagaaa attaatataa
     1201 ctattaaaca tattaaaccg tttgttttaa tggcttacgt tcgccaattt gttgccgcca
     1261 cattgcataa tacaatccct tctcgtctaa taaacgctga tgattgccac tttcgattat
     1321 ttggccttgc tctaaaacat aaataacatc ggcatgcatt atagtagata aacgatgtgc
     1381 aattaaaatg gtaatatggt tacgcccatc agaaatgttt cttatggttt gactaatttc
     1441 ttcttcagtt atactatcca atgctgaggt ggcttcatca aaaaccaata agtcaggtgc
     1501 acgcaataat gctctggcaa ttgataagcg ttgcttttcg ccacccgaaa cttttacacc
     1561 gccttcgcca atcattgtat ccaagccttt atcagcacgt tgcagcaaac tggtacaggc
     1621 cgctttttgc aacacatcca tgcattgcgc atcggtggcg gcaggattta caaataacaa
     1681 attttcgcgt atggtacctg caaacaattg cgtgtcttgc gttacaaaac ctatttggct
     1741 gcgcaagtca ttaaaattaa tttctgtacc cggtatgtcg ttgtacttaa taactccctg
     1801 ctgtggtttg taaagtccca caagta
//
LOCUS       DAOMAA010000447         1825 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_67645_length_1825_cov_2.337853, whole genome shotgun sequence.
ACCESSION   DAOMAA010000447 DAOMAA010000000
VERSION     DAOMAA010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67645_length_1825_cov_2.337853"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            295..648
                     /locus_tag="PLO59_06505"
     CDS             295..648
                     /locus_tag="PLO59_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQV00793.1"
                     /translation="MLTHIHPKLPMRNLNNTKQYYINQLGFKQTGGDYNGYLMLEKDG
                     IEIHFFEFANLNPDENYGQVYIRCSDIYTLHKHLQQKGVPMHPQSTVELKPWGQIEFA
                     LLDPDNNLLTFGQSV"
     gene            710..>1825
                     /locus_tag="PLO59_06510"
     CDS             710..>1825
                     /locus_tag="PLO59_06510"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HQV00794.1"
                     /translation="MKFICGFLALFIASAQAQTVYPLMPNIEVDSILAVKPGATKIAY
                     DALSNHLYYSTVDGNIYRVNMLGTGAATDTLMYTASNHGITFLQGLFFKDSTLFVCGN
                     NWGAVMTIGMVVKGVLQPNGMRQWINVVTTDPYPMASISGDHGFSAVHVDPTGNYIFV
                     SSGARTHLGEVRTFFGSCPNCREIPITSKIFRFPVQSVGLTLPNDSTALSNSGYLFAS
                     GTRNAYAMAWDANQNLFAIDNSGERDDPEELNWIRQGHHYGFPWRMGGNDNPLRFPPY
                     DVNQDALVNPLCAGYINGWFANDTTFPPVPQGVTFTDPIRNYGVDADYFRTPTGQVNN
                     ASDMGSYITTFTAHRSPLGLVTDADSVLPAPYTGLAFV"
BASE COUNT          534 a          378 c          371 g          542 t
ORIGIN      
        1 agctaccgta ctgcaattgt ttgaaggcga aaaggctaac atggctattg ccaaagcaca
       61 atcaataggt gccattatgc aatataaggt tacacaaacc agtatgcttt aaacaaataa
      121 agggtaggaa gttttaccaa tactacattt aaacgtagcg tactccggtt tcaaacattt
      181 caatgcgatt agtgatgaaa ttttaaaaat aggtttctgt ttttaagtca gtcagccatt
      241 tgcaaattta tcagttgtga ttggtataag ccattaatta attattttag atgtatgctt
      301 acacacatac atcccaaatt gcccatgcgt aatttgaata atacaaaaca atattacatc
      361 aaccagttgg gctttaagca aaccggaggt gattataacg gctatttgat gcttgaaaaa
      421 gatggaatag aaattcattt tttcgagttt gctaatctta accccgatga aaattatggc
      481 caggtatata ttcgttgcag cgatatttac accttgcata agcacctgca acaaaagggc
      541 gtacccatgc atccgcaatc gacagttgaa ttaaaaccct ggggtcagat tgaatttgca
      601 ttgctcgacc ctgataacaa cttacttact tttggccagt ccgtttaatt catagctatc
      661 caacaatcgt tttgaaaata ttaatcgtta aaacttaact tacactacca tgaaatttat
      721 atgcggcttt ttagccttat tcatagcaag tgcacaggca caaacagttt atccattgat
      781 gcccaatatt gaagtagatt cgatattagc tgttaagccc ggtgctacaa aaattgctta
      841 cgatgccctg agcaaccatt tgtattatag taccgttgat ggtaacatat atcgcgttaa
      901 catgttaggt accggtgctg caaccgatac attaatgtac acagcaagca accatggcat
      961 aactttttta caaggattgt tttttaaaga cagtacatta tttgtttgcg gcaataattg
     1021 gggtgcagta atgactattg ggatggttgt aaaaggggtg ttgcaaccca acggtatgcg
     1081 ccaatggatt aatgttgtaa ccaccgatcc ttatccgatg gcaagcatca gtggcgacca
     1141 tggtttttcg gctgtgcatg ttgacccaac cggtaactat atttttgtat cgagtggcgc
     1201 ccgtacccac cttggcgaag tgcgtacatt cttcggctct tgccccaatt gccgcgagat
     1261 acctataact tcaaaaatat tcaggtttcc ggtacaaagc gtaggcctta ccttgcctaa
     1321 cgattcaacg gcattaagca acagcggtta cttatttgcc agtggtacgc gcaatgctta
     1381 tgctatggca tgggatgcaa accaaaatct gtttgccatt gataactcag gcgaacgtga
     1441 cgaccccgaa gaattaaact ggatcagaca ggggcaccat tatgggtttc cgtggcgcat
     1501 gggtggtaat gataacccac tacggtttcc gccttatgat gttaatcagg atgcattggt
     1561 taatccactt tgcgcaggtt atataaatgg ttggtttgcc aacgacacca catttccgcc
     1621 tgtaccgcaa ggtgtaactt ttaccgatcc tatacgcaat tatggtgttg atgccgatta
     1681 ttttagaaca cctacaggcc aggtaaataa tgccagcgac atgggcagct acattactac
     1741 ttttacagca cacaggtcgc cattaggttt ggtaacagat gccgatagtg ttttgccggc
     1801 accctatacc ggccttgctt ttgtt
//
LOCUS       DAOMAA010000448         1823 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_67757_length_1823_cov_3.376131, whole genome shotgun sequence.
ACCESSION   DAOMAA010000448 DAOMAA010000000
VERSION     DAOMAA010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67757_length_1823_cov_3.376131"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..124)
                     /locus_tag="PLO59_06515"
     CDS             complement(<1..124)
                     /locus_tag="PLO59_06515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQV00795.1"
                     /translation="MISVVCPVLNEAEYMAQLIEFFIAAKPTVKELIIVDGNSTD"
     gene            complement(121..1203)
                     /locus_tag="PLO59_06520"
     CDS             complement(121..1203)
                     /locus_tag="PLO59_06520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQV00796.1"
                     /translation="MKRILFLSDINSPHTQKWVSGLASQGFMMGVFSLTPSQTNWYKH
                     LPNVLLLNESTISKKTFTAGIYGKLQYLSLLPQLKKVVKSFKPNLLHANYASSYGLLG
                     ALLNFKPFIITVWGSDVFDFPQNHFINRSILKYNLRKADVIVSASKIMCSETAKYTNT
                     QVIHIPFGIDANVFVPSVTKSNRLLIGTIKRLEYIYGVDILIKAFALIADKVSADLLI
                     VGNGVSMNQYRTLAKSLNIESRVKFTGWIPHEQTLQYHQMINIFVAVSRRESFGVAVL
                     EASACGNPVLVSNAGGLPEVVANQITGIIVEPDDVTATANALLQLCNDAALRNSMGQA
                     GRKFVIKHYNWPDNLMQMVQLYNQYV"
     gene            complement(1392..>1823)
                     /locus_tag="PLO59_06525"
     CDS             complement(1392..>1823)
                     /locus_tag="PLO59_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00797.1"
                     /translation="YRYFKSVSKVNPRNVLAFWTYLDWLVSGSLPYMTLPALGYDQRS
                     RAGRGYSQGRFRGEKLFYAETEFRFNISPCTKVLGGVVFVNVITTSSELADVKLFEYV
                     KPAVGAGLRLMVDKKGRTNLFMDYAFGKQSNGFYFGATETF"
BASE COUNT          586 a          368 c          288 g          581 t
ORIGIN      
        1 tatctgttga gttaccatca actattatta attcttttac tgttggcttg gcagcaataa
       61 aaaactcaat tagctgtgcc atatattcag cttcatttaa aaccgggcat acaactgata
      121 tcatacatat tgattataaa gttgcaccat ttgcatcaag ttatcaggcc agttataatg
      181 tttgataaca aatttacgac cggcttgtcc catgctgttc cttagcgctg catcattgca
      241 taattgcagc aatgcgtttg cagttgctgt tacatcatcc ggctcgacaa taatacctgt
      301 aatctggttt gcaacaactt ctggtaggcc gcctgcattg ctaaccaata cagggttacc
      361 acatgctgat gcctcaagca cggctacacc aaaactctct cgtctggaaa cggcaacaaa
      421 aatatttatc atttgatgat attgtaaggt ttgttcatgt ggtatccagc ctgtaaattt
      481 taccctgctt tctatgttta aacttttggc caatgttcga tattgattca ttgaaactcc
      541 gttgccaaca attaataaat cagccgaaac tttatcggca attaatgcaa aagcttttat
      601 taaaatatct actccataaa tatattccag acgcttaatt gtacctatta acagcctgtt
      661 tgattttgtt acacttggta caaatacatt tgcatcaatg ccaaatggta tatgaattac
      721 ctgtgtgttt gtgtacttag cagtttcgct acacattatt ttgctagccg aaacaattac
      781 atcggctttg cgcaaattat actttaatat acttctgttt ataaaatggt tttggggaaa
      841 atcaaataca tcgctacccc aaaccgtaat gataaacggc ttaaaattta ataaggctcc
      901 taaaagtcca tagctacttg catagtttgc atgtaataaa tttggtttaa acgattttac
      961 aaccttttta agctgtggta acaagcttag atattgcaac ttgccataaa tacccgctgt
     1021 aaatgttttt ttgctgatgg tactttcatt tagcaataat acattgggta aatgcttgta
     1081 ccaattggtt tgacttggcg ttagcgaaaa aacacccatc ataaaacctt gtgatgccaa
     1141 cccgcttacc catttttgag tatgtggcga gttaatatca cttaaaaaca aaattcgttt
     1201 catgtaaggt aactgaaaac tgctgcaagt tagtgaatga cttcgccact tagataattt
     1261 ataaatcaga ttttaaacaa taacaaaata tattaatcgc atttgaaaag cacaagattg
     1321 tgtatctcca ttgataacac ttgcattacg aacatgttca attaaaaata tgtatcggga
     1381 ggttacacct gttaaaaagt ttcggtagca ccaaaataaa atccattcga ttgtttaccg
     1441 aatgcataat ccataaataa gttagtacgc ccttttttat caaccataag ccttaagccc
     1501 gcccccacag ccggtttaac atattcgaaa agtttaacat cagctaactc agatgaggtg
     1561 gttattacat ttacaaatac aacgcctcct aaaactttcg tacatggcga tatattaaac
     1621 cgaaattcag tttcggcata aaaaagtttt tcgcctctga aacggccttg cgaataaccc
     1681 ctgccggcac gacttcgttg atcataacct aatgcaggta aagtcatata aggcaatgaa
     1741 cctgatacca accaatccaa ataagtccaa aatgccaaca cattacgcgg atttactttt
     1801 gaaaccgatt tgaaatacct gta
//
LOCUS       DAOMAA010000449         1822 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_67824_length_1822_cov_3.110922, whole genome shotgun sequence.
ACCESSION   DAOMAA010000449 DAOMAA010000000
VERSION     DAOMAA010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67824_length_1822_cov_3.110922"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /gene="def"
                     /locus_tag="PLO59_06530"
     CDS             <1..531
                     /gene="def"
                     /locus_tag="PLO59_06530"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010231173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HQV00798.1"
                     /translation="DAVLRKKAENITNDYKALPQLINDMFETMYNANGVGLAAPQIGL
                     AIRLFVIDASPFAEDEKELENFKRVFINAQILDEAGEAWKFNEGCLSIPGVREDVLRK
                     PDITITYLDENFDSKTEKFSGLAARIIQHEYDHIEGILFTDKLNPLRKRMIKGKLNDI
                     SNGKVNVDYRMRFIRK"
     gene            541..1074
                     /locus_tag="PLO59_06535"
     CDS             541..1074
                     /locus_tag="PLO59_06535"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HQV00799.1"
                     /translation="MKENIFLIGFMGCGKSTIGKQLAKLMQYDFVDLDIEIELQNQIT
                     ITELFLKGETYFRIAEQACLHQVAQKQKQVVAVGGGAPCFYNNLELMNDSGLTIYLSM
                     PPAALHSRLKNAKDKRPLLTNVPDNKLQSFITSLLQQREPFYLKANLHVASLNCKAGY
                     LFQLIEMAGWFHKNKYN"
     gene            1108..1458
                     /locus_tag="PLO59_06540"
     CDS             1108..1458
                     /locus_tag="PLO59_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HQV00800.1"
                     /translation="MNQERVYRIVFASVYPLYIQKATSKGRSKDEVDQILYWLTGYDS
                     KTLQLQIESKVDLETFFAEAPMFNNNASKITGVICGYRVEEISDPLMQKIRYMDKLID
                     ELAKGKKMDKILRS"
     gene            complement(1512..>1822)
                     /locus_tag="PLO59_06545"
     CDS             complement(1512..>1822)
                     /locus_tag="PLO59_06545"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HQV00801.1"
                     /translation="GSTFEDKFINGTSLDFSFYRGIPPNTSSEDEEESELYKIGDTVI
                     VKFCTMDKKSYGFWREAETQSGNNGNPFGSISPINSGVTGGLGIFCAYSPSYDTLICK
                     "
BASE COUNT          622 a          308 c          340 g          552 t
ORIGIN      
        1 gatgcagttt tacgtaaaaa agccgaaaac ataacaaacg attacaaagc cttaccccaa
       61 ctgattaacg atatgtttga aaccatgtat aatgccaatg gcgttggttt ggctgcacca
      121 caaattgggt tagctatcag gttgtttgtt attgatgcat cgccttttgc cgaagatgaa
      181 aaggagttag aaaactttaa acgtgttttt attaatgccc aaatacttga tgaggcaggc
      241 gaggcctgga agtttaacga aggatgttta agcatacctg gtgtgcgcga agatgtgtta
      301 cgcaaacccg atattaccat tacatatttg gatgaaaact ttgattcgaa aaccgaaaaa
      361 tttagtggtt tggctgcacg cattattcag catgaatacg atcatattga aggcatactt
      421 tttactgata aattaaatcc gctgcgtaaa cgtatgatta aaggtaaact taacgatata
      481 agcaatggca aagttaatgt ggattatcgc atgcgtttta tcagaaaata acggttacct
      541 gtgaaagaaa acatttttct gattggattt atgggttgcg gcaaaagcac tataggaaag
      601 caattagcca agctcatgca atatgatttt gttgatttag atattgaaat agagctgcaa
      661 aaccaaatta ccattaccga acttttttta aaaggcgaaa catattttag aatagcagaa
      721 caggcatgtt tgcatcaggt tgcacaaaaa caaaaacaag ttgttgctgt tggtggtggt
      781 gcgccatgct tttataataa tttggaatta atgaatgaca gtggccttac aatatattta
      841 agtatgccac ctgcagcatt acatagccgg ttaaaaaacg caaaggataa aaggccatta
      901 cttactaacg tgcccgacaa taagctgcaa tcttttataa cctcactttt gcaacaacgc
      961 gaaccatttt atttaaaagc taacttgcat gttgcttcat taaactgtaa agcaggttat
     1021 ttgtttcaat taattgaaat ggccggatgg tttcataaaa acaaatacaa ctgataaact
     1081 gctttttaaa atatttattt ttgaattatg aatcaggaac gtgtttatcg aattgtgttt
     1141 gccagtgttt atccactgta tattcaaaag gcaacatcta aagggcgcag taaggacgaa
     1201 gttgatcaaa tactatattg gcttacaggt tatgattcaa aaacgctcca attacaaatt
     1261 gaaagtaaag ttgacctgga aacatttttt gccgaagcac ccatgtttaa caataacgca
     1321 tcaaaaatta cgggtgtaat ctgcggttac cgtgttgaag aaattagcga ccctttaatg
     1381 caaaaaataa ggtatatgga taagttgatt gatgaattag caaaaggaaa aaagatggat
     1441 aaaattctta gaagctaaag tcactcatgt aataactttt gttaaccaca accgtttaca
     1501 ttttaaaaac attacttaca aataagtgta tcgtatgaag ggctgtaagc acaaaaaatt
     1561 cctaacccac ctgtaacacc actgtttata ggcgatatag aaccaaatgg attgccattg
     1621 ttgccgctct gcgtttcggc ttcgcgccaa aatccgtatg attttttatc catcgtacaa
     1681 aattttacta tcaccgtatc gccaatttta tatagttccg actcttcttc atcttcgcta
     1741 ctggtgttgg gtggtatgcc acggtaaaac gaaaaatcta atgaagtgcc atttataaat
     1801 ttatcttcaa aagtagatcc aa
//
LOCUS       DAOMAA010000450         1822 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_67853_length_1822_cov_2.422750, whole genome shotgun sequence.
ACCESSION   DAOMAA010000450 DAOMAA010000000
VERSION     DAOMAA010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67853_length_1822_cov_2.422750"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="PLO59_06550"
     CDS             complement(<1..567)
                     /locus_tag="PLO59_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007652384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HQV00802.1"
                     /translation="MGLFDILTQEIAIDLGTANTLIIHNDKVVVDEPSIVAIDRTTGK
                     VLAVGKQAQMMHGKTHENIKTVRPLKDGVIADFDAAEHMIRGMIKMINLGSKVFQPSL
                     RMVICIPSGITEVEKRAVRDSAEHAGAKEVYLIHEPMAAAIGIGIDVEEPMGNMIIDI
                     GGGTSEIAVIALGGIVCDQSIRVAGDGFT"
     gene            complement(606..>1822)
                     /gene="purH"
                     /locus_tag="PLO59_06555"
     CDS             complement(606..>1822)
                     /gene="purH"
                     /locus_tag="PLO59_06555"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HQV00803.1"
                     /translation="DLYPFEATVAANADAQQIIEKIDIGGISLIRAAAKNHEHVLIIP
                     SMADYDWLLQVLQNQNGTTTLSQRLKQAHKAFGVSAHYDMAIAQWLMQQDNISPQPIT
                     VNAPAMHLRYGENPHQQGVYYGDFNALFDKLSGKDISYNNLLDIDAAINLITEFDTTT
                     VIILKHNNACGCASKPTLIQAWQAALAGDPVSAFGGVIITNTLVDADAATAINELFFE
                     VLIAPGYSTEALQILTAKKNRIILKQHTYQKPLLQYRSALNGLLQQDTDLQTEGVAQM
                     KLVTTTAPTTTQLTDLAFALKICKHSKSNTIVLAQNNQLLGSGVGMTSRIDALQHAIN
                     KAKAQGFDLQNAVMASDAFFPFPDCVQIAHQAGITAVVQPGGSIKDALSIDYCNKNSV
                     AMLTTGVRHFKH"
BASE COUNT          473 a          382 c          385 g          582 t
ORIGIN      
        1 tgtaaaacca tcgccagcaa cgcgtattga ttggtcgcaa acgataccgc ctaaagcaat
       61 aactgcaatt tcgctggtcc caccaccgat gtcaattatc atattgccca taggttcttc
      121 tacatcaata cctataccta tggccgctgc catgggttca tgtatcagat acacttcttt
      181 ggcacctgca tgttcggcac tatcgcgtac ggcacgtttc tctacttctg ttatacccga
      241 tgggatacag ataaccatgc gcagcgatgg ttgaaaaacc ttactgccca ggtttatcat
      301 ttttatcatg ccgcgtatca tgtgctcggc tgcatcaaaa tcggcaatta cgccatcttt
      361 taatgggcgt acggttttta tgttttcatg ggttttgcca tgcatcattt gtgcctgctt
      421 gcctacggcc aacaccttgc ctgtagtgcg gtcaatagct acaatagatg gctcatcaac
      481 aactacttta tcgttatgaa tgattagggt attggctgtg cctaagtcaa tggcaatttc
      541 ttgcgttagt atatcaaaaa gtcccatgag aaaatttgct tattattatt tataggaggt
      601 aaattttaat gtttaaaatg tcggacaccg gttgtaagca ttgcaacgct atttttgtta
      661 caataatcta ttgacaaagc atcttttata gaaccacccg gttgcactac tgctgttatg
      721 cctgcttgat gggcaatttg aacgcaatcc ggaaacggga aaaatgcatc gcttgccatt
      781 acggcatttt gcaaatcgaa gccttgtgct tttgctttat tgatggcatg ttgcaaagca
      841 tcaatacgac tggtcatacc tacgccactg cctaatagct gattgttttg tgccagcaca
      901 atggtatttg acttactgtg tttgcatatt ttaagtgcaa aagcgaggtc ggtaagttgt
      961 gtagttgttg gtgcagttgt ggtaaccagt ttcatttgcg ccacaccttc ggtttgcaaa
     1021 tcggtatctt gttgcaacaa gccgtttaat gcgctgcggt attgtagcaa cggtttttga
     1081 tacgtatgct gctttaatat gatgcggttc ttttttgctg ttaaaatttg taatgcttct
     1141 gtgctgtaac cgggtgcaat taaaacttca aaaaacagtt cgttaatagc ggttgctgca
     1201 tctgcatcaa ccaaggtgtt tgtaatgata acaccgccaa aagcgcttac agggtcgcca
     1261 gccaaagcag cctgccaagc ctggataagt gtaggtttgc tggcacaacc gcatgcattg
     1321 ttatgcttta aaattattac agttgttgta tcgaactcag ttattaagtt tatggctgca
     1381 tcaatatcta acaggttatt gtacgaaata tccttgcccg aaagcttatc aaacaaggca
     1441 ttaaaatcgc cataataaac accctgttgg tgcggatttt cgccatagcg caaatgcata
     1501 gccggtgcgt ttaccgttat gggttgcggg ctgatattat cttgttgcat cagccattgt
     1561 gcaatagcca tatcataatg tgccgatacc ccaaaagctt tgtgtgcttg ttttaacctt
     1621 tgcgataatg tagtggttcc attttgattt tgtaaaacct gaagcaacca gtcataatca
     1681 gccattgatg ggataattag aacatgctca tgatttttag ccgcagctct gataagcgaa
     1741 atgccgccta tgtctatttt ttcaataatc tgttgggcat cagcattggc ggctacggtg
     1801 gcttcgaatg gatataaatc aa
//
LOCUS       DAOMAA010000451         1821 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_67891_length_1821_cov_3.329558, whole genome shotgun sequence.
ACCESSION   DAOMAA010000451 DAOMAA010000000
VERSION     DAOMAA010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67891_length_1821_cov_3.329558"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="PLO59_06560"
     CDS             complement(<1..741)
                     /locus_tag="PLO59_06560"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HQV00804.1"
                     /translation="MFQFAHHQSLLIRKLVLLFFLGITNTLYAQRNVILIIADDIGTD
                     YFGCYENHKDTINAPNLNRLLNKGVRFKNATANPICSPTRAGIFTGRYCFRTGVGTSV
                     GDGNANATPLDTNELSLPRLMRLNFQNQIKMAHIGKWHLHQDTPATAMLIPNKMGYDF
                     YSGNFKALVNNYYHWKKITNGSYQMINNYATVETTNDAVSWIKSQSTNPFFLWVSYNA
                     PHEPYHYAPTNLHSFPNLSGTPQDINNNP"
     gene            complement(792..1517)
                     /locus_tag="PLO59_06565"
     CDS             complement(792..1517)
                     /locus_tag="PLO59_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HQV00805.1"
                     /translation="MQDFSWLQRTSLLVGDEKVKALSQKHVMIVGMGGVGSYAAEFIA
                     RSGIGIITIVDGDVVDPTNRNRQLPALSTNHGQSKAEIMAQRLLAINPELKLTVIKEF
                     ITPDAVAQLLLLNPNYIIDAIDSITPKLTLLTQAFNLKIKVVSSMGAGAKLDPTQLQV
                     VDISKTYNCPFAQQIRKTLRKHGIYKGITAVFSPETPIRSSLMLTDGTNYKKSAYGTI
                     SYLPATFGAVTASVVIRHFLTSN"
     gene            complement(1519..>1821)
                     /locus_tag="PLO59_06570"
     CDS             complement(1519..>1821)
                     /locus_tag="PLO59_06570"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HQV00806.1"
                     /translation="KDYPKVHAAFHGFNKNVSLAQQITKRGYYLSFGEALAHPRNQET
                     LKHINLKQVLLETDYATIQIEKVYTLAAQTLAMQLDRLQEQIFDNCEHFFGKKLIL"
BASE COUNT          536 a          312 c          354 g          619 t
ORIGIN      
        1 tggattgttg tttatatcct gaggtgtacc gcttaaattc ggaaacgaat gcaagttggt
       61 aggcgcgtaa tgataaggtt catgtggtgc attataggac acccataaaa agaatggatt
      121 ggtgctttgc gattttatcc aactaacagc atcattggta gtttctacag ttgcataatt
      181 atttatcatt tgataactgc cattggtaat ctttttccaa tggtagtagt tatttaccag
      241 cgctttaaag ttgccactat aaaaatcata acccattttg ttgggtataa gcatggctgt
      301 tgcaggtgta tcctgatgca aatgccattt acctatatga gccattttaa tttggttttg
      361 aaaatttaaa cgcatcaaac gaggtaagct taattcattt gtatctaacg gtgttgcatt
      421 ggcgttgcca tcgcccaccg aagtacctac accggtacga aaacaatagc gcccggtaaa
      481 aataccggca cgtgttggcg aacaaatagg gttggctgtt gcatttttaa accgaactcc
      541 tttgtttaac aggcggttta aatttggtgc attaatagta tccttatgat tttcgtagca
      601 tccaaaataa tctgtaccaa tatcgtcagc aattatcagt atcacatttc gttgcgcata
      661 tagggtattg gtaatgccca aaaaaaatag caataccaat tttctgatta ataaagattg
      721 atgatgtgca aattgaaaca taatggtttt gttcttaacg aatggctgtt taaagtttaa
      781 agaaatggat attagttaga ggttaaaaaa tgtcgaataa ctacagatgc agttactgcg
      841 ccaaatgtag caggtaaata tgatatcgta ccataagccg attttttata gttggtacca
      901 tcggttagca ttaagctgct gcgtatgggt gtttcgggcg aaaatacagc ggtaatacct
      961 ttgtaaatac catgttttcg tagcgttttg cgaatttgtt gtgcaaatgg acaattatag
     1021 gttttcgaaa tatctactac ttgtaattgt gtaggatcta attttgcgcc agctcccata
     1081 ctgcttacta ctttaatttt tagattaaat gcttgcgtca gcagtgtaag cttaggggta
     1141 atgctatcta tggcatcaat aatataatta gggtttaata gcaacaattg cgcaacagca
     1201 tccggagtaa taaactcttt aataacggtt aactttagtt cgggatttat ggccaataat
     1261 ctttgcgcca ttatttcggc tttactttgg ccatgattgg ttgataaggc cggtaattgg
     1321 cgatttcggt ttgttgggtc aactacatca ccatcaacaa tggtaatgat accaatgcca
     1381 ctacgagcaa taaattcggc agcataacta cccacaccgc ccatgcctac tatcataaca
     1441 tgtttttgcg atagagcctt tactttttca tcgcccacta atagagaagt acgttgtaac
     1501 cagctaaaat cctgcatatt ataaaataag ttttttgcca aaaaagtgtt cgcaattgtc
     1561 aaatatttgt tcttgcaacc tatctaattg catggctaat gtttgggctg caagggtgta
     1621 aactttttca atttgtatgg ttgcataatc tgtttctaac aaaacttgtt tcaggtttat
     1681 atgttttaag gtttcttgat ttcttggatg tgctaatgct tcaccaaatg ataaataata
     1741 accgcgtttt gttatttgct gggctaacga tacattttta ttaaatccat gaaatgcagc
     1801 atgcactttt ggataatctt t
//
LOCUS       DAOMAA010000452         1818 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_68123_length_1818_cov_2.871242, whole genome shotgun sequence.
ACCESSION   DAOMAA010000452 DAOMAA010000000
VERSION     DAOMAA010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68123_length_1818_cov_2.871242"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..881
                     /locus_tag="PLO59_06575"
     CDS             <1..881
                     /locus_tag="PLO59_06575"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HQV00807.1"
                     /translation="KTQFMIYTISNQQIQISVKQAGAELCSIKNLTGTEFMWQAGAQW
                     QRHAPVLFPIVGKLKDNGFNYNDKKYALTQHGFARDMLFNCVAKTDTITCTLIDDAFT
                     QEKYPFKFKLDIIYSLLSDGVLVSYRITNTGSQLMPFSVGGHPAFNCPLLADEKFEDY
                     EIVFDKSASLSSLMLQNGLFDGSVKHLASDSNRFSLHKHIFDDDALVFENLHAKHVKL
                     LSKKTGSFVEMSLEGFPYLGIWSKPGAPFVCLEPWCGRADSVNANGNLFDKTGIEILA
                     PGAQFERTYSIKVFSC"
     gene            complement(882..1532)
                     /locus_tag="PLO59_06580"
     CDS             complement(882..1532)
                     /locus_tag="PLO59_06580"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013663371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HQV00808.1"
                     /translation="MHFKQFKLAATLIFTVLLADQLFKFWVKTNMYLGQEFHIAGQWF
                     IIHFVENEGMAFGLSFGGNYGKLALSLFRVAAVCGIGYYLYKLIKEGVHTMYVTSLSL
                     IFAGAIGNIIDSIFYGVLFSSSEFQVATFLPAEGGYATWMHGRVVDMLYFPLIRGTFP
                     DWFPLWANQSFEFFRPVFNLADSSITIGVCLFLIFQKQIFKSEQAKNTATPTQDFQ"
     gene            complement(1617..>1818)
                     /locus_tag="PLO59_06585"
     CDS             complement(1617..>1818)
                     /locus_tag="PLO59_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HQV00809.1"
                     /translation="LAKEEAAQLAARQRKFIEQLENALIRIKNKTFGICRVTGKLIPK
                     DRLRAVPHTTQSIEAKLLQNRD"
BASE COUNT          582 a          329 c          357 g          550 t
ORIGIN      
        1 gaaaaacaca attcatgata tataccatca gtaaccagca aattcaaatt tctgtgaagc
       61 aagcaggagc cgagctttgc agcattaaaa acctaacagg tacagagttt atgtggcagg
      121 caggtgcgca gtggcagcgg catgcaccgg tgttatttcc aatagttgga aagttaaaag
      181 acaacggttt taattataac gataaaaagt atgcgttaac tcagcatggt tttgcacgcg
      241 atatgctgtt taattgtgtg gccaaaacag atacgataac ctgtacccta atcgatgatg
      301 catttacaca ggaaaaatac ccgtttaaat ttaagttgga tattatttac agtttacttt
      361 cagatggtgt attggtgagt taccggataa ccaatacggg tagtcaactg atgccttttt
      421 cagttggtgg gcatccggct tttaattgcc cgctgttagc agacgaaaaa tttgaagact
      481 atgaaattgt ttttgataaa agtgcaagct tatcatcatt gatgcttcaa aatggtttgt
      541 ttgatggcag tgttaagcac ctggcttctg attcgaaccg tttctcatta cacaaacata
      601 tttttgatga tgatgcactt gtgtttgaaa acctgcatgc taaacatgta aaattgttat
      661 caaaaaaaac cggaagtttt gtggaaatga gtttggaagg atttccttac ttaggtattt
      721 ggagcaaacc cggtgcgccc tttgtttgtt tagaaccctg gtgtggtagg gctgatagtg
      781 taaatgccaa cggtaattta tttgataaaa ccggaattga aattttagcg cccggtgcgc
      841 aatttgaacg cacttactcc attaaagttt ttagctgcta actattgaaa atcctgcgtt
      901 ggtgtagctg tattttttgc ctgttcggat ttaaaaattt gtttttggaa aattagaaat
      961 aagcatacgc ctatggttat cgaactatcg gccaaattaa atacaggcct gaaaaattcg
     1021 aacgattgat tagcccaaag aggaaaccaa tcaggaaaag tgccacgtat taaagggaag
     1081 tataacatat ccacaacacg gccatgcatc caggttgcat agccaccttc ggccggtaaa
     1141 aaggttgcaa cctgaaactc gctactgcta aatagtacac cataaaatat actgtcgata
     1201 atattcccaa tggcacctgc aaatattaat gataatgagg taacgtacat ggtatgcaca
     1261 ccttctttta ttagcttata caaataatag cctatgccac aaacagcagc cactctaaat
     1321 aaacttaatg ccagcttgcc gtaattgcca ccaaatgaaa gtccaaatgc catgccttcg
     1381 ttttcaacaa aatgaataat aaaccattgg ccggcaatat gaaattcctg ccctaaatac
     1441 atattggttt ttacccaaaa tttaaaaagc tgatcggcta aaagcactgt aaaaataagt
     1501 gtagcagcca gcttaaattg cttaaaatgc atcagtatta ttttctaacg ggattaaaaa
     1561 actaacggca acgaagttga atcattgccg ttaagtttgt attgtttatt tatatgctaa
     1621 tcgcggtttt gaagtaattt ggcttcaata ctttgggtag tatgtggtac agcacgtaaa
     1681 cggtctttag gtataagttt accggttaca cgacaaatac caaaagtttt atttttaatc
     1741 cgaatcaaag cattttcaag ttgctcaata aacttgcgtt ggcgcgcagc taattgtgca
     1801 gcttcttctt ttgctaaa
//
LOCUS       DAOMAA010000453         1817 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_68196_length_1817_cov_2.947219, whole genome shotgun sequence.
ACCESSION   DAOMAA010000453 DAOMAA010000000
VERSION     DAOMAA010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68196_length_1817_cov_2.947219"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            260..>1817
                     /locus_tag="PLO59_06590"
     CDS             260..>1817
                     /locus_tag="PLO59_06590"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HQV00810.1"
                     /translation="MSKLQFKFVIVFMIVALLGLISLQVYWISHDIKIKEDQFEYRAN
                     TALQEAVDKFESYRALNYLTATESDSGQKKMGNNLPAAKHRSNMESSGLHLDSSSRSR
                     MHSSIRVFKKNNKEIVTFYDNQIIITPGTDVFQKFKDNLDWDQLQSEDFYFYPIDPSD
                     ENIRGTRKNTERRNNILKVDSDKNESQSFTINDASSGSKMKVDYGTSSGFNYVISYKT
                     IQRKRDSLLHEFEGISSQTEKVILANQMEAGRDKIDKITKRYQELLNKASKELGQNNT
                     ELTPQNYLMLDTLIKRAMINNCIALPYQYAVVDITTDSILYANSKKTKSEITKSLIRA
                     QLFPNDLIEKPIMLMINFQGKFRFVLANIWPVLLSAALFTIIIVLTSAYTIYVILRQK
                     KLAIIKNDFINNMTHEFKTPLATIALAADSIRNPKVYENIDKLMFYTDIIKTENIRMN
                     AQVTNVLQTAQLDKGELKMQMVAVNMHQLITEVADRHKLQIVNRNGTLNINLQAEAFI
                     INADKLHMSNA"
BASE COUNT          663 a          301 c          325 g          528 t
ORIGIN      
        1 cattaatgtt tttttcaatt ttaattctta tttgattctg ctgccctaat gcaatacctg
       61 caatggcaac aaaagcagta caacctaaaa gtttcacggt gttataaatt agatttttca
      121 taagcgtttg ttttttgttt gtttgatatc tcaaacgtaa aagcagtaag agttgctagt
      181 ggttaatggc aggttaatgc tgctgttaat agttggttaa ccgtaaacgg ccaaagttta
      241 cccgtataaa tttgagggca tgagtaaatt gcaatttaaa tttgttatcg tttttatgat
      301 agtggctttg ctgggattaa tatccttaca agtgtattgg ataagccacg atattaaaat
      361 taaggaagat cagtttgaat acagggctaa tacagctttg caagaagctg tagataagtt
      421 tgagtcatac agggcactca attatttaac tgcaacagaa tccgatagtg gtcaaaaaaa
      481 aatgggcaac aacttgcctg ccgccaaaca tagaagcaac atggagtcga gcggcttaca
      541 tttagacagc tcatcgcgaa gccgtatgca tagcagtatc agggtcttta agaaaaacaa
      601 taaagaaatt gttacctttt atgataacca gataataatt acacctggta ccgatgtatt
      661 tcaaaagttt aaagacaatt tggattggga tcaattgcaa agcgaagact tttattttta
      721 tccgattgac ccctccgatg aaaatatacg cggtacaaga aaaaacaccg aacgaagaaa
      781 taatatttta aaagttgatt cggataaaaa cgaaagtcaa tcgtttacta taaacgatgc
      841 atccagtgga tcgaaaatga aagttgacta tggtacctca tcagggttta attatgtgat
      901 aagctataaa accatacaac gcaagcgcga ttcattgttg catgaattcg aaggcatttc
      961 atcacaaacc gaaaaagtca tattagcaaa tcagatggag gcaggccgtg ataaaattga
     1021 taaaattacc aaacgctatc aggaactatt aaacaaagcc tcaaaagagt taggccaaaa
     1081 taataccgag ctaactccac aaaattattt gatgctcgat accttgataa aacgtgccat
     1141 gataaacaat tgtattgcat tgccttatca atatgctgtt gttgatatta ctaccgattc
     1201 aatattatat gcaaacagta agaaaactaa atcagaaata actaaaagct taatacgtgc
     1261 gcaattattt ccgaacgatt taattgaaaa gccaataatg cttatgataa attttcaagg
     1321 caagtttcgg tttgtattag ctaatatttg gcctgtgcta ctaagtgctg ccttatttac
     1381 tataataatt gttttaacat cggcctatac aatttatgta atactgcgac agaagaaact
     1441 ggcaataatt aaaaatgatt ttataaataa tatgacgcat gagtttaaaa caccccttgc
     1501 taccattgca ttagcagccg atagcatccg gaatcctaaa gtgtatgaaa acatagataa
     1561 acttatgttt tatactgata ttataaagac agaaaacata cgcatgaatg cccaggtaac
     1621 caatgtatta caaacagcac agttagataa aggcgagtta aaaatgcaaa tggttgccgt
     1681 aaacatgcat caacttatta ccgaagtagc agacaggcac aaactgcaga ttgtaaacag
     1741 aaacggtacc ttaaatatta atcttcaggc cgaggcattt ataataaatg cagataagct
     1801 tcacatgagt aatgcaa
//
LOCUS       DAOMAA010000454         1813 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_68476_length_1813_cov_3.418089, whole genome shotgun sequence.
ACCESSION   DAOMAA010000454 DAOMAA010000000
VERSION     DAOMAA010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68476_length_1813_cov_3.418089"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            226..915
                     /locus_tag="PLO59_06595"
     CDS             226..915
                     /locus_tag="PLO59_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HQV00811.1"
                     /translation="MLLQISVGTPAADTLATTSNPPIPDMPLWEIVQSGGPIMIPIAL
                     LSVIAVFIFFERYFTISKRGKIDMNFMHQIRDHVHNGNIEAARTLCKNTNTPVAAMIE
                     KGIMRLGKPIRDIETAIENVGKLEIYKMERYLNILGVIAAIAPMFGFLGTIFGVIKIF
                     YQIALQNSLDLGVISGGLYVKMVTSAAGLTVGIMAYVLYHILTGMIDRVVNKMEINAV
                     EFIDLLEEPVK"
     gene            932..1333
                     /locus_tag="PLO59_06600"
     CDS             932..1333
                     /locus_tag="PLO59_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019946684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HQV00812.1"
                     /translation="MDLKRRKRYVGEVYTSSLTDIMFFLLLFFLITSAFATPTVLKLL
                     LPNAKTGTQGVKHPIVIAIDADLNYAIGTTRIAATDLEATLTREIAGQVDPAALIKVD
                     KTVPVQALVDLLEIGNRLKIKMVLATANSKN"
     gene            1390..>1813
                     /locus_tag="PLO59_06605"
     CDS             1390..>1813
                     /locus_tag="PLO59_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00813.1"
                     /translation="MEATIRNKSILLTLLIHAILVALMFVFALSTPNPPFADAGGGGM
                     LVSIGTVDEASGQIQATSEITSTQPQQTEPIPQPASSNAEEIITTENENTVAVKPVEK
                     TNKKPTETKTVKPVETKVNPTATTKTPERQLNQSALFKG"
BASE COUNT          634 a          369 c          316 g          494 t
ORIGIN      
        1 caaacaatta cacgaataaa aacatttagt aaatttaata cctgccaata caacaataaa
       61 aagtttagat actgcgttta cctgctaaca agccactgtt agtaaataac atattgcaaa
      121 cagtgtaaac acaagccccc tactactttc gctacttaat agcgaaccat aaacgaaacc
      181 gctataggca ttttttacaa gtaaattttt aaaacttcaa cttatatgct gttgcaaatt
      241 tcagtaggca cacctgcggc cgatacctta gctaccacat caaatccgcc catacccgac
      301 atgccattat gggaaattgt gcaaagtggt ggccccatta tgatacccat tgctttacta
      361 tcggtaatag ctgtatttat tttttttgaa cgctatttta ccattagcaa acgtggcaaa
      421 attgacatga actttatgca tcaaatacgc gaccatgtgc ataatggaaa tatagaagca
      481 gctcgcacac tatgtaaaaa cacaaacaca cctgttgcag caatgattga aaaaggcatt
      541 atgcgtttgg gcaaacccat tcgcgatatt gaaacagcaa ttgaaaatgt tggtaaatta
      601 gaaatctaca aaatggaacg atacctgaat atattaggtg ttattgccgc tattgcgccc
      661 atgtttggat tcttgggaac catatttgga gtgataaaaa tattttatca gatagcttta
      721 caaaacagtt tggatttggg cgttatatcc ggtggcttgt atgtaaaaat ggttacctcg
      781 gctgcaggtt taacggtagg tataatggct tatgtgctgt atcacatact taccggcatg
      841 attgacagag tagtaaataa aatggaaata aatgctgttg agtttattga cttattagaa
      901 gaacctgtaa aataaaaaca acacaaataa catggactta aaacgaagaa aacgatatgt
      961 gggcgaagtt tatacctcgt cacttaccga cattatgttt tttttattgc tgttctttct
     1021 catcacttct gcatttgcta cgcctacggt attaaaatta ttactgccga atgcaaaaac
     1081 cggtacacaa ggcgttaaac atcctattgt tattgccatt gatgccgatt taaattacgc
     1141 aattggaacc acacgcatag ctgcaaccga tttagaagca acgcttacac gcgaaattgc
     1201 cgggcaggtt gatcctgctg ccttaattaa agtggataaa acagtacccg tgcaagcctt
     1261 agttgatttg ttggaaatag gcaaccgttt aaaaattaaa atggtgctgg ctaccgctaa
     1321 ttcaaaaaat taaataataa gcctgccaat aattaaatta cctatatggt aactgttaca
     1381 acataaatta tggaagcaac catcagaaat aaatccatac tgcttacatt gcttatacat
     1441 gcaatattgg tggcattgat gtttgtattt gccttatcaa cacccaaccc accttttgcc
     1501 gatgccggtg gtggtggcat gttggtaagc attggcaccg ttgacgaagc atcaggtcaa
     1561 atacaagcaa cttctgaaat aacatctaca caaccacaac aaaccgagcc cataccacag
     1621 ccagccagca gcaatgccga agaaataatt acaacagaaa acgaaaacac agttgctgtt
     1681 aaacctgtag aaaaaaccaa taaaaaacct acagaaacaa aaaccgttaa gccggttgaa
     1741 accaaagtaa acccaactgc cactaccaaa acacctgaac gacagcttaa ccaaagtgca
     1801 ctgtttaaag gtt
//
LOCUS       DAOMAA010000455         1811 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_68670_length_1811_cov_2.683371, whole genome shotgun sequence.
ACCESSION   DAOMAA010000455 DAOMAA010000000
VERSION     DAOMAA010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68670_length_1811_cov_2.683371"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..>1811
                     /locus_tag="PLO59_06610"
     CDS             17..>1811
                     /locus_tag="PLO59_06610"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQV00814.1"
                     /translation="MSIPKEQFPDIKIATIYVTTFNYGTSPTDVENLITKPLEKQIKG
                     IAGIKKMTSNSVQDFSNIMIEFNTDVSEADAKQKVKDKVDLARKDLPNDLSDEPNVIA
                     VDFSEFPMLSVNIAGDYDLDKLKSYADDIKDKIEAMREIRRVDMVGDLEREIQINVDM
                     YKMQAAQISMRDIENAVRFENMTISGGNVKMDGMERSLSVKGEFKNVDQINNLVVNST
                     AGSPIYLRDVATVVDAHKEQESYARLYGKNVITLNVIRSTGENLVEASDKITAMLNEM
                     KTTTLPKDVEVSVTGDQSTSTKVTLHDLINTIIIGFILVTIVLMFFMGATNALFVAMS
                     VPLSMFVAFLVLPAIGFSLNMIVLFAFLLALGIVVDDAIVVIENTHRIFENGKVPIVQ
                     AAKMATGEVFLPVLSGTLTTLAPFIPLAFWSGLIGKFMVFLPVTLIITLLASLLVAYI
                     INPVFAVDFMKPHNDAEHKKPLKQKSFKVTATIFGVIAAISYLIAFTSHSAGAFGMGN
                     FAVFIILLVVFFRLFLEKRILHFQKVTWPAVQSKYESILRWALARPYTMIFSTVALFF
                     LSIVFLIIRQPQVIFFPQADPNFVYVYVTLPV"
BASE COUNT          565 a          321 c          371 g          554 t
ORIGIN      
        1 cggtatctta tcttatatga gcatacctaa agagcaattc ccggatatta aaatagcaac
       61 catatatgtt accactttta attacggcac atcgccaacc gatgtagaaa accttattac
      121 aaaaccactc gaaaaacaaa taaaaggcat tgcaggtatt aaaaaaatga ccagcaactc
      181 cgtacaggat ttttcgaata ttatgataga gtttaatacc gatgtaagcg aagccgatgc
      241 caagcaaaaa gtaaaagaca aagtggattt ggcacgtaaa gatttgccta acgatctatc
      301 ggacgaaccc aatgtaattg cagttgactt tagcgaattt ccgatgctaa gtgtaaatat
      361 tgccggtgat tatgatttgg ataaattaaa atcgtatgcc gatgatataa aagataaaat
      421 tgaagccatg cgcgaaataa gacgtgtgga tatggtaggc gacctcgaac gcgaaataca
      481 aattaatgtg gacatgtata aaatgcaggc cgcacaaatt agcatgcgcg atattgagaa
      541 tgccgttcgg tttgaaaaca tgaccataag tggtggcaat gtaaaaatgg atggcatgga
      601 acgttcgctg agtgtaaaag gtgaatttaa aaacgttgac cagataaata acctcgttgt
      661 aaattcaact gccgggtcgc caatttattt acgcgatgtg gccacagtag ttgatgcaca
      721 taaagaacaa gaaagttatg cgcgcctgta cggtaaaaat gtaataacct taaacgttat
      781 acgcagtaca ggcgaaaatt tagttgaagc atcggataag ataacggcca tgctaaacga
      841 aatgaaaacc actacactgc ccaaagatgt tgaggttagc gtaacaggcg atcaaagtac
      901 cagcaccaag gttacactac acgatttaat taataccatc atcattggtt ttatattggt
      961 aaccatagta ctcatgtttt ttatgggtgc taccaatgca ttgtttgtag ctatgagtgt
     1021 accgttaagc atgtttgttg catttttggt tttacctgct attggcttta gtttaaacat
     1081 gattgtattg tttgcgtttt tattagcctt gggtattgtg gttgacgatg ccattgtggt
     1141 aatagaaaac acacaccgta tttttgaaaa tggcaaggtg cccattgtac aagcagctaa
     1201 aatggctacc ggtgaggtgt ttttgcccgt actgtcgggc acattaacca cacttgcacc
     1261 atttatacca ttagcatttt ggagtggtct gattggtaag tttatggtgt ttttaccggt
     1321 aactttaata ataacactgt tggccagttt gttggtagcc tatatcatta acccggtatt
     1381 tgctgttgac tttatgaaac cgcataacga tgcggaacac aaaaaaccgt taaagcaaaa
     1441 atcgtttaaa gttacggcaa ccatatttgg tgtaattgct gccattagtt acctgattgc
     1501 ctttacttca cattcggcag gtgcatttgg catgggtaat tttgccgtat ttataatttt
     1561 actcgtagtg tttttccgat tgtttttaga aaaacgcatt ctccattttc aaaaagttac
     1621 atggcctgca gttcaaagta aatacgaaag tatattacgc tgggcattgg cacgccctta
     1681 taccatgatt tttagtacgg ttgcattgtt ctttttatcc attgtgtttt taataataag
     1741 gcaaccacag gtaatatttt tcccgcaagc cgatcctaat tttgtttatg tatatgttac
     1801 cctgcctgtg g
//
LOCUS       DAOMAA010000456         1807 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_68946_length_1807_cov_2.803082, whole genome shotgun sequence.
ACCESSION   DAOMAA010000456 DAOMAA010000000
VERSION     DAOMAA010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68946_length_1807_cov_2.803082"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="PLO59_06615"
     CDS             <1..329
                     /locus_tag="PLO59_06615"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020526432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HQV00815.1"
                     /translation="QMFYDNNKYYNFVNHCKESGIQVPIIPGIKVLTSKKQITNLPKV
                     FHIDLPQELIEEVVKCKTDADVKNVGIEWAIQQSKDLIKFGVPCLHYYTMGNPDIIKR
                     VASQVF"
     gene            388..1071
                     /locus_tag="PLO59_06620"
     CDS             388..1071
                     /locus_tag="PLO59_06620"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HQV00816.1"
                     /translation="MWADFVNLIFPDRCRACQNNLNPKDNMICMRCRLTLPRTNYHLD
                     VNNPVSKHFWGKTHLSYATAFLFFQKGDRVQHLIHQLKYKGDKEIGVVIGSLFGNALN
                     ATDTFSKATILIPVPLHSSRKHARGYNQSEMLAKGLAQKMNIPVNTKLLVRSQKTETQ
                     TNKRRYNRYENMRDVFEVISPHLYTNQHFLLVDDVITTGSTLVACADKLLTIEGASVS
                     VAAMAFAQH"
     gene            complement(1107..>1807)
                     /locus_tag="PLO59_06625"
     CDS             complement(1107..>1807)
                     /locus_tag="PLO59_06625"
                     /inference="COORDINATES: protein motif:HMM:NF021916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HQV00817.1"
                     /translation="TALAVNPKIVCVSAGGKVIEIAKQHQLDHIIIPGGMPPRACFAY
                     SFTQLFYVLHGMGLIDDAFKAELAATVQLLSAEVEAIKAEANQLANKLLGTIPAIYST
                     ASYEGVCVRFRQQINENSKMLCWHAALPEMNHNEIVGWVEKCPNVSVVFMRNADDYYR
                     TQKRMDFLKEVALQCTPHVFDVWSKGETMLARVYYLVLFGDWVSVYLSELKNIDPTEV
                     KVIDRLKQTLNALS"
BASE COUNT          600 a          346 c          321 g          540 t
ORIGIN      
        1 cacaaatgtt ttatgataac aacaagtact acaactttgt aaatcactgc aaagaatcag
       61 gtattcaagt acccattata ccgggcataa aagtgcttac atctaaaaaa caaattacca
      121 acttacctaa ggtatttcat attgatttgc cgcaagaatt aatagaagaa gtagtaaaat
      181 gtaaaaccga tgcagatgta aaaaatgtag gtattgaatg ggctattcaa caatcgaaag
      241 acttaataaa atttggcgtg ccgtgtttgc attattacac catgggtaat cctgatataa
      301 ttaaacgtgt agcatcgcaa gttttttaga caaaaataaa atcggcttta tctattgttt
      361 atattgcagt aataacatat accaaatatg tgggctgact ttgtaaattt aattttccct
      421 gatcgttgtc gcgcctgtca aaataattta aatccgaagg acaacatgat ttgtatgcgt
      481 tgcaggttaa ccttgccgcg aaccaactac catttagatg taaataatcc ggtaagcaaa
      541 catttttggg gcaaaacaca tttaagctat gctacggcat ttttgttttt tcagaaaggc
      601 gatagggtac aacatctaat acatcagtta aaatataagg gcgataaaga gataggggta
      661 gttataggca gcttgtttgg caatgcatta aatgcaaccg atacttttag taaagcaacc
      721 atactgatac cggtaccgct gcatagcagc cgaaaacatg cacgcggcta taatcaaagc
      781 gaaatgttgg ccaagggttt ggcacaaaaa atgaacatcc ctgtaaatac aaagctcctg
      841 gtaaggagcc aaaaaacgga aacccaaacc aataaacgca gatacaatcg ctacgaaaat
      901 atgcgcgatg tttttgaagt aataagtcca cacttatata caaaccaaca ttttttatta
      961 gttgatgatg ttatcactac cggatcaacg ctggttgctt gtgccgataa attattaact
     1021 attgaaggcg caagcgtaag tgttgctgct atggcttttg cacagcacta attcgaataa
     1081 agtaattgaa tgggtttaat cagcttttat gacaaagcat taagagtttg cttaagcctg
     1141 tcaataacct ttacctcagt tgggtctatg ttttttaact cgcttaaata tacactaacc
     1201 caatcgccaa ataaaaccag ataatacaca cgggctaaca tggtttcgcc tttactccaa
     1261 acatcaaata catgtggtgt acattgtagt gctacttcct ttaaaaagtc catacgtttt
     1321 tgtgtacgat aataatcatc ggcattgcgc ataaaaacta cagaaacatt cggacatttt
     1381 tctacccaac ctacaatttc attgtggttc atttcgggta atgccgcatg ccaacacaac
     1441 attttactat tctcatttat ttgttgccta aatctaacac atacaccttc ataagatgcc
     1501 gtactgtaaa tggctggtat ggtacccaaa agtttatttg ccaattggtt ggcttcggct
     1561 ttaatggctt caacttctgc gcttagcaat tgaacagtgg ctgctaattc ggctttaaat
     1621 gcatcatcaa ttaagcccat accatgcaat acatagaata attgtgtaaa cgaataagca
     1681 aagcaagcac gtggtggcat gccacccggt atgataatat gatcaagctg atgttgtttg
     1741 gctatttcaa ttactttacc tccggcactt acacatacta ttttcggatt tacggctaat
     1801 gccgttt
//
LOCUS       DAOMAA010000457         1803 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_69235_length_1803_cov_2.831236, whole genome shotgun sequence.
ACCESSION   DAOMAA010000457 DAOMAA010000000
VERSION     DAOMAA010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69235_length_1803_cov_2.831236"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..134
                     /locus_tag="PLO59_06630"
     CDS             <1..134
                     /locus_tag="PLO59_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015807161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HQV00818.1"
                     /translation="TKHYPGQNVGIGKDHTLFALADGKVVFRKKADDRSYVSIEAQA"
     assembly_gap    400..409
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(516..863)
                     /locus_tag="PLO59_06635"
     CDS             complement(516..863)
                     /locus_tag="PLO59_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010182704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HQV00819.1"
                     /translation="MDIEWIRIYCLQKQAVTECLPFDENTLVFKVMDKMFLLLDLNGN
                     TFNTKCNPEYAIELRDTYEAITPGYHMNKKYWNTIVYSELPNNIVLALIDHSYNEVVA
                     GFTKKQKLALNHH"
     gene            complement(872..>1803)
                     /locus_tag="PLO59_06640"
     CDS             complement(872..>1803)
                     /locus_tag="PLO59_06640"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV00820.1"
                     /translation="PAVNNVNLPVPGTATLTANGQHVIKWYDDAMATNLVHVGNTFTT
                     PFLNNNTAYYAADVFLAPGQSGFVGPANNTVGASALNNSNQRYLVFSAYKPCKINFVT
                     AYAGNPGNRTIELRDANGNVLQSKTFSLTTGANLLNLDFTVNPGVNYQLGLSATSIAD
                     LHRNSSGITYPYQLNNLLDITGSSAGSAYYYFFYNWQVSEPDFECSSPLAVGQVTIGP
                     VGINDFDFERTIQVFPNPTNNMVQVAFAVNEFTEAKIIVSDITGKQVYSYSNNQKAGV
                     FNHHLNLSKLLAGVYQLSIYVNNKPYHQKLIKY"
BASE COUNT          542 a          315 c          339 g          597 t
ORIGIN      
        1 gtacaaaaca ttacccaggt caaaatgttg gtatcggaaa agaccatact ttatttgctt
       61 tggcagatgg taaagttgtg ttccgcaaaa aggcagacga tcgctcttac gtttcaatag
      121 aagcacaggc gtaatcaata aaagatttat aataaaagcc ccacttggtt atccaagtgg
      181 ggctttttta ttgcgttgtt ttattaaaaa taaaactgta aaggcataaa aaaaaccggt
      241 ggcaaaggcc ttatccggtt ttcatgaaaa taattattac ccctaatcct tactttctga
      301 tagaaataaa ggtaaacatt tttatttcat tttaattgct taacattcat tcattaccaa
      361 ataatatttt atcattatat tttattttta ttgttgtttn nnnnnnnnnc cctaatcctt
      421 actttctgat gcaaataacg gtaacatttt tatttcattg taagtgcttc acatttattc
      481 actaacaaat aactgtttat cattaaaatt tcattttaat ggtggttcag ggccagtttt
      541 tgtttcttag taaagcctgc aactacttca ttgtaggaat gatcaatcaa ggcgaggaca
      601 atgttgttgg gcaattcgct atacacaatg gtgttccaat attttttatt catatggtaa
      661 ccgggggtta tggcttcata cgtatctctc agctctattg catattcagg attacatttt
      721 gtattaaaag tgttaccatt taagtcgagc agcaaaaaca ttttatccat tactttaaaa
      781 accagggtgt tttcgtcaaa aggcaagcac tctgttaccg cttgtttttg caaacaataa
      841 atgcggatcc actcaatatc catgtggggt attagtactt aattagtttt tgatggtatg
      901 gtttgttatt tacgtaaata gaaagttgat atacaccggc aagcagtttg cttaaattta
      961 aatggtgatt aaaaacaccg gccttttgat tgttactata actatacacc tgtttaccgg
     1021 taatatctga tactataatt ttagcttcgg taaattcatt tactgcaaat gcaacttgta
     1081 ccatattgtt tgtagggttg ggaaatacct gaatggttcg ttcaaaatcg aagtcattaa
     1141 ttcctactgg gccaatagta acttggccaa ctgcaagcgg actgctgcat tcaaagtcgg
     1201 gttctgatac ttgccagtta tagaagaagt aataatacgc actgccggct gatgagcctg
     1261 tgatatccag taaattgttt aattggtagg gatatgttat accactgctg tttcgatgca
     1321 aatcggcaat agaagtagcc gataaaccca attgataatt aactccgggg ttaaccgtaa
     1381 aatcaaggtt taaaaggttg gcaccagtag taaggctaaa ggtttttgat tgaagtacat
     1441 tgccatttgc atcgcgtaac tcaatggttc tgttgcccgg gttgcctgcg tatgcggtaa
     1501 caaaatttat cttacatggt ttatatgccg aaaaaaccag atagcgttgg ttactattgt
     1561 ttaatgccga tgcaccaaca gtattatttg caggcccaac aaagcccgat tggcctggtg
     1621 ctaaaaatac atcggccgca taataggctg tattattgtt tagaaatggc gtagtaaacg
     1681 tgttgccaac atgcactaaa ttggtcgcca ttgcatcatc ataccacttt ataacgtgtt
     1741 ggccattagc cgtaagtgta gctgtgcccg gcactggcaa attaacatta ttgacagcag
     1801 gtg
//
LOCUS       DAOMAA010000458         1801 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_69361_length_1801_cov_2.786942, whole genome shotgun sequence.
ACCESSION   DAOMAA010000458 DAOMAA010000000
VERSION     DAOMAA010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69361_length_1801_cov_2.786942"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1658
                     /locus_tag="PLO59_06645"
     CDS             <1..1658
                     /locus_tag="PLO59_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016194069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HQV00821.1"
                     /translation="FRMYHKLAGMTGTAETEAGELWEIYKLDVVTIPTNRPIIRKDHE
                     DQVYRTTREKYKAVIDEIVKLTQAGRPVLVGTTSVEISELLSRTLRANNIKHNVLNAK
                     QHQREAEIVAEAGKAGTVTIATNMAGRGTDIKLGPGVREAGGLAIIGTERHESRRVDR
                     QLRGRSGRQGDPGSSQFFVSLEDNLMRLFGSERIAKLMVRMGLEDGEVIQHSMITKSI
                     ERAQKKVEENHFGTRKRLLEYDDVMNAQREVIYKKRRHALYGERLQLDMLNMQYDLAD
                     VIVKNAHDENDYAQLTADCIRYFGMECPITNEEFSGTKNAESLTDKMFDAAHQHYRNK
                     GVAVAKHTYPIIENVFQQQGQTIENIVVPFSDGLRQMQVMVNLKKAYQSQGAELIAAL
                     ERNITLAVIDDEWKEHLRAMDDLKQSVQNAVYEQKDPLLIYKFESFKLFQQVLESINK
                     DILSFVFKGFIPMQNPEQVREAKPLPKADNTKLKATHADANAVPAQVASNTSSTQAPR
                     YANDNMPKPKAQPVRAEQKIGRNDPCPCGSGKKFKNCHGQNQD"
BASE COUNT          598 a          354 c          400 g          449 t
ORIGIN      
        1 actttaggat gtaccacaaa ctggccggta tgaccggtac agccgaaacc gaagcaggcg
       61 aattgtggga gatatataaa ttagatgtag ttaccatacc aactaacaga cccattatac
      121 gtaaagacca cgaagaccag gtatatcgta ccacacgcga aaaatataaa gctgtaattg
      181 atgaaattgt aaaactaacc caagccggca ggccggtttt agtaggtaca accagtgtcg
      241 aaatttcaga gttgttatcg cgcaccttac gtgccaataa cattaaacac aatgtactta
      301 atgccaaaca acaccaacgc gaagccgaaa tagttgccga agccggtaaa gccggtactg
      361 taaccattgc caccaacatg gccggcaggg gtaccgatat taagttaggc cccggtgttc
      421 gtgaggctgg tggtttagcc ataattggta cagagcgtca cgagtcgagg cgggttgaca
      481 ggcagttgcg cggtaggtcg ggccgtcagg gcgacccggg ttcatcacag ttctttgtgt
      541 cactcgaaga taatttaatg cgcttatttg gtagcgaacg tattgccaaa ttaatggtgc
      601 gtatgggttt agaagatggc gaagtgatac aacacagtat gattaccaag tcaattgaac
      661 gtgcacaaaa gaaggtagaa gaaaatcatt tcggcacacg taaacgtttg ctcgaatacg
      721 atgatgtaat gaatgcacaa cgcgaagtga tttataaaaa acgaagacat gcattgtatg
      781 gcgaacgatt acagttagat atgttgaata tgcaatacga tttggctgat gtgatagtta
      841 aaaatgcgca tgatgaaaac gattatgcac aactaacagc cgattgtatc aggtactttg
      901 gtatggaatg tccgattaca aatgaagaat tttcgggtac caagaatgcc gaatcgctta
      961 ccgataaaat gtttgatgca gcgcatcagc attaccgtaa taaaggcgtt gctgtggcaa
     1021 aacatacata cccaataatt gaaaatgtat ttcagcagca aggccaaacc atcgaaaata
     1081 ttgttgtgcc ttttagtgat ggcttacgtc aaatgcaggt tatggttaat ctgaaaaaag
     1141 catatcaaag ccagggtgca gagttaattg ccgcattaga acgcaacatt acactggccg
     1201 taattgatga tgagtggaaa gaacatttac gtgccatgga cgatttaaaa caaagtgttc
     1261 aaaatgctgt atatgaacaa aaagatccgc tgttgattta taagttcgag tcgtttaagt
     1321 tgtttcaaca agtattggaa agtattaata aggatatatt atcatttgta tttaaaggtt
     1381 ttattcccat gcaaaatcct gagcaggtgc gcgaagcaaa gccattaccc aaagctgata
     1441 ataccaaatt aaaagcaaca catgccgatg ccaatgctgt gccggcacag gtcgcttcca
     1501 atacgtcaag cacacaagca ccgcgctatg caaatgacaa catgcccaaa cccaaagcac
     1561 agcccgtgcg tgccgaacaa aaaataggac gtaacgaccc atgcccttgt ggtagtggca
     1621 agaaattcaa aaactgccat ggacaaaatc aggattgatt tgtaaacagg ccttacataa
     1681 aatgcgcatc taaacgatgt gcattttttt ttctaataaa ccacataaat aaatcctttt
     1741 ctgaaaatct gctgcttgtc aactccgtta atacgtggtg tatatcttac ggtgtaggca
     1801 t
//
LOCUS       DAOMAA010000459         1795 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_69746_length_1795_cov_3.232759, whole genome shotgun sequence.
ACCESSION   DAOMAA010000459 DAOMAA010000000
VERSION     DAOMAA010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69746_length_1795_cov_3.232759"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..976
                     /locus_tag="PLO59_06650"
     CDS             <1..976
                     /locus_tag="PLO59_06650"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HQV00822.1"
                     /translation="YGEYVKSPQVAYAHVLAQHLPPNLQSCYFVNSGSEAIEGALKLA
                     KRVTGRSQIIAFNGAYHGSTHGALSLMSDTYFTSAFRPLLPDITFIDWNNLNQLKLIT
                     SKTACVVTEVVRAESGAITPQKGFLQALQAQCNAVGALLICDEIQTGFGRTGPLFGFM
                     DYNIQPDIICLGKGMAGGMPLGAFIASNERMNCLSHNPVLGHITTFGGHPVSCAAAIA
                     NLEVIKSLDTEKTIPEKEKIIRTHLKHKAIQSISGKGLMLAVSFKDETFNKSVIKYCI
                     ENGVVVDWFLFADNKLRIAPPLIIENDLLEKACFTILDGIEYTYRKLN"
     gene            993..1670
                     /locus_tag="PLO59_06655"
     CDS             993..1670
                     /locus_tag="PLO59_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008242820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQV00823.1"
                     /translation="MTVLIVEDEKSLSHEIEIFLTKQGYNCDVAFNAKSGVDKASSNG
                     YDFILLDIGLPDGNGFDVLTQVKSNGVESSIIILTARGEVDDKILGLQSGADDYLAKP
                     FSLPELFARMQAITRRKHGLKNTVIKIYDFDMDVNNRTVSHNSNLINLTKKEFDLLYY
                     MTLNKNRVLTRLQLTEHIWGDVIDDDNESNYIDVHIKNLRKKLSTQANAEFIETVRGL
                     GYRVNMQ"
BASE COUNT          624 a          281 c          348 g          542 t
ORIGIN      
        1 ttatggcgag tatgttaaaa gcccacaagt agcttatgca catgtattgg cgcaacactt
       61 accacccaat ttgcagtcgt gttattttgt caactcaggc agcgaagcca ttgaaggagc
      121 attaaaatta gctaaaaggg ttacaggccg cagtcaaata atagcattta atggcgcata
      181 tcatggtagt acacatggtg cactgagcct tatgagcgat acatatttta cttcggcatt
      241 caggccgcta ctacctgata ttacttttat tgattggaat aatttaaatc aactgaagtt
      301 aattacatca aaaactgcat gcgttgtaac cgaggtagtg cgtgccgaaa gtggcgccat
      361 tacacctcaa aaaggttttt tacaagcctt acaagcacaa tgcaatgctg tgggggcttt
      421 attaatttgt gatgaaatac aaaccggttt cggacgtact ggcccgttgt ttggttttat
      481 ggattacaac atccaaccag atattatttg cttaggtaaa ggtatggcag gaggtatgcc
      541 attgggtgcg tttatagctt caaatgaaag aatgaattgt ttatcacata acccggtatt
      601 agggcatatt accacatttg gtggccatcc tgttagttgt gctgcagcaa tagctaacct
      661 ggaagtgata aaaagtttag atacagagaa aaccattccg gaaaaagaaa aaattatcag
      721 aacccattta aaacataagg caatacaatc aatttcaggc aaaggattaa tgctggctgt
      781 tagctttaaa gatgaaacat ttaataaatc agtaataaaa tattgtatcg aaaatggagt
      841 agtagtagat tggtttttat ttgctgataa taaattacga atagcaccgc cattaataat
      901 tgaaaatgat ttattggaaa aagcttgttt tacaatatta gatggaattg aatatactta
      961 tagaaaactt aattaactta aaacctaaaa caatgacagt actaattgta gaagacgaaa
     1021 aaagcctatc gcatgaaata gaaatatttt tgaccaaaca aggttataac tgtgatgtag
     1081 catttaacgc caaaagtggt gttgataaag ccagtagtaa cggctatgat tttatattgc
     1141 ttgatattgg tttgcccgat ggaaatggct ttgatgtttt aactcaggta aaatcaaatg
     1201 gtgttgaaag cagtattatt attttaactg ccagaggcga agtggacgac aaaattttag
     1261 gccttcaatc aggtgcagat gattatctgg caaagccatt ttcattaccc gaactttttg
     1321 cgcgtatgca agctataaca cgcagaaaac atggattaaa aaatacggta attaaaattt
     1381 acgattttga tatggatgta aacaaccgta ctgtatcgca taacagtaat ttaattaatt
     1441 taaccaaaaa ggagtttgat ttactgtatt acatgacctt aaataaaaac cgtgtgctta
     1501 cccgattaca acttaccgag catatatggg gtgatgttat tgacgatgac aatgaaagca
     1561 attacataga tgtgcatata aaaaatttgc gaaaaaaact atccacacag gcaaatgccg
     1621 aatttattga aaccgttaga ggtttaggtt accgtgttaa tatgcaataa aaaagcgcac
     1681 tttgcagtgc gctttttaaa aagtgcattg taaaaaatac ttttgttgca tggtgcaaat
     1741 attatgggcc ttacccattg ttatttttat aaattatata atcggcaaat ccgat
//
LOCUS       DAOMAA010000460         1794 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_69849_length_1794_cov_2.266245, whole genome shotgun sequence.
ACCESSION   DAOMAA010000460 DAOMAA010000000
VERSION     DAOMAA010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69849_length_1794_cov_2.266245"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1794
                     /locus_tag="PLO59_06660"
     CDS             <1..>1794
                     /locus_tag="PLO59_06660"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00824.1"
                     /translation="SPVGSSDNYNEFSNNQILNGSFAIYWYGAASPLYETGNMIMSNT
                     ISGQNTFGIQAGNQIDLAIHNNIIDEAAATFGTGIYMSTCVGNCAVMANKIFLQQNGT
                     GINLQQCGNTGAGEVMVANNFVTIGSGGNLAYGIRVNTCTNTGIYYNNVHNISSVTNT
                     SNYAFYAEGVANNVKVLNNVFMAVSGYIAGATTGSISQMDNNDLFTTGFSFCNFNNVS
                     QTNLANWQTATGWDANSVNVDPQFINATDLHIQTFGLNAEATPIAAILVDIDGQQRDV
                     NTPDIGADETAPPSNDAGVTKLVSPHKSQCGGNTVTVKAIVRNYAALPLTNVKVMAEI
                     TGTQNANLLDSLAGPLGVAQSDTITFTQTLNSNITGDTLYIKLYTMLDNDEYLSNDTL
                     MVDSLIFAPVPSAPLVPGTTDICLGTTTSINATLGTNQQVYWFDAAVLGNQIASGNPL
                     VVNPTINTNYYAEVRDTTLGSGGCIRITEVNLAGNGAFGDYIELTNLSGGLIDATGWT
                     VATSDNYTSINTTNAITWSLGVFNPGEIQIRYDGSGAGSNYWGTNLLYNPGNNGWVII
                     VDNLGNVVDFVAFGWTAADLAGFNITVNGFNI"
BASE COUNT          546 a          370 c          368 g          510 t
ORIGIN      
        1 tcaccggtag gttcatcgga taattacaat gagtttagca acaatcaaat tctaaacggc
       61 tcgtttgcta tttattggta tggtgctgcc agcccgctat atgaaacggg caatatgata
      121 atgagcaata ccataagcgg ccaaaatact tttggcattc aggccggcaa tcaaattgat
      181 ttggcaatac acaacaacat tattgatgaa gctgcagcaa catttggtac aggtatttat
      241 atgagcactt gtgtaggcaa ttgtgctgta atggctaata aaatattctt acaacaaaat
      301 ggtacaggta taaacctgca gcaatgtggt aatacgggcg ctggcgaagt aatggttgca
      361 aacaattttg taactattgg ctctggtggt aatttggctt acggtattcg tgttaacacc
      421 tgtaccaaca ccggcattta ttacaataac gtacacaaca tttcttcggt aacaaacaca
      481 agcaactatg cattttatgc cgaaggcgtt gctaacaatg taaaggtatt aaacaatgta
      541 tttatggcgg tttcgggcta tattgcaggt gctactaccg gcagtatttc acaaatggat
      601 aataacgatt tgtttaccac cggtttcagt ttttgtaact ttaacaatgt aagtcaaact
      661 aacttggcaa actggcaaac agctactggc tgggatgcaa actcagttaa tgtagatcct
      721 caatttatta acgctaccga tttacacatt caaacctttg gcctcaatgc cgaagccaca
      781 cccattgctg ctattttagt ggatattgat ggccagcaac gtgatgttaa cacacctgat
      841 attggggccg atgaaaccgc accgcccagc aatgatgctg gtgttaccaa attggtttcg
      901 cctcataaaa gccaatgtgg tggcaataca gttaccgtaa aagccatagt acgcaactat
      961 gcagcattac cattaaccaa tgtaaaagtg atggccgaaa taaccggtac gcaaaatgca
     1021 aacctgctcg actccttagc cgggccgtta ggtgtggcac aatctgacac cattacgttt
     1081 acacaaacat taaattcaaa tataacgggc gatacacttt acattaaact atacaccatg
     1141 cttgataatg acgaatactt aagtaacgac acacttatgg ttgactcgct tatttttgca
     1201 cctgtaccct cagcaccgct tgtacccggc actaccgata tttgcttagg caccaccact
     1261 tcaataaatg ccaccttggg taccaaccaa caagtttact ggtttgatgc agcggtatta
     1321 ggcaatcaaa ttgcatcggg caacccactg gttgtaaacc ctactataaa cacaaattat
     1381 tatgccgaag tacgcgacac caccttgggt agcggtggat gcatccgtat aactgaagta
     1441 aacttagcag gtaatggcgc ctttggtgat tatattgaat taaccaattt atcaggcggt
     1501 ttgattgatg caaccggctg gacagtagcc accagcgata actacacatc aataaatacg
     1561 acaaatgcta ttacctggag tttaggggtg tttaatccgg gcgaaattca aataagatat
     1621 gatggctctg gtgccggtag taattattgg ggtactaatt tattatataa tcccggcaat
     1681 aacggttggg taataattgt agataactta ggtaatgttg ttgattttgt tgcttttggc
     1741 tggactgcag ccgaccttgc cggatttaat attaccgtaa atggttttaa tatc
//
LOCUS       DAOMAA010000461         1793 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_69903_length_1793_cov_3.075374, whole genome shotgun sequence.
ACCESSION   DAOMAA010000461 DAOMAA010000000
VERSION     DAOMAA010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69903_length_1793_cov_3.075374"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="PLO59_06665"
     CDS             complement(<1..350)
                     /locus_tag="PLO59_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009185241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00825.1"
                     /translation="MNLFRFLAPAIFITSNVWAQAPKYSNEFLSIGLGARAQAMGGAV
                     VASVTDVTAAYWNPAALTQTNANLQIGLQHAELFAGISKYDYAGLAAQIDATRTIGFS
                     VIRSAVDDIIDSTNL"
     gene            495..1274
                     /locus_tag="PLO59_06670"
     CDS             495..1274
                     /locus_tag="PLO59_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002698971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HQV00826.1"
                     /translation="MNLLKHIPNALTCCNLLCGCLGIIFLFEGSLLWAAYCLPIAAVF
                     DFFDGFVARFFKVNSAIGKDLDSLADTVTFGVLPALMLYHYFKIGMAVHGVIQPAWLP
                     YIALLLAVFSALRLAKFNNDSRQTTSFIGVPTPAISLFIGSWPLFMQNPAMQNLNGTN
                     AWQYLQQMDVAQPAWQNMLLHPWFLLPLTLLMCFLLVCELPLFALKFKSFTWAENKQR
                     YMFLTGSAILLLIFKFAAIPIIITVYVLLSLVMHATAKQKS"
     gene            1277..1528
                     /gene="purS"
                     /locus_tag="PLO59_06675"
     CDS             1277..1528
                     /gene="purS"
                     /locus_tag="PLO59_06675"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016195127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HQV00827.1"
                     /translation="MIFSASIQVMPLKALLDPQGKAVSASMKNLNLSAIENVRIGKNI
                     TLQVTADTETEALNMVTEACKKLLANPIMEEFEISIKAL"
BASE COUNT          503 a          364 c          353 g          573 t
ORIGIN      
        1 aggttggtac tgtcaattat atcgtcaaca gccgagcgta taaccgaaaa gccaatggtg
       61 cgtgtagcat caatttgtgc ggctaagcct gcataatcat atttgcttat gccggcaaac
      121 agctcggcat gttgcaggcc aatttgcagg ttggcattgg tttgtgtaag cgctgccgga
      181 ttccaatatg ctgccgtaac atctgttacc gaagcaacca ctgcaccacc cattgcctgc
      241 gcacgcgcgc ccaggccaat agacaaaaac tcattactat actttggcgc ttgcgcccat
      301 acattgcttg taatgaagat agccggagcc aaaaacctaa ataaattcat gccgctttaa
      361 ataagaaaga ggtaacgtta aattactttt tatgttgcct actttggctt acgaaggtaa
      421 cagaatttgc aacctgcata ataatctgat atcagatgcc aaacttttac acctttgtag
      481 caaataatga ttttatgaat ttactcaaac acataccaaa tgcacttaca tgctgcaatt
      541 tgctttgcgg ctgtttgggc attatttttt tgtttgaagg cagcttattg tgggctgcct
      601 actgcctgcc tatagcagct gtattcgatt tttttgatgg ttttgttgcg cgttttttta
      661 aagtaaacag tgctattggt aaggatttag attcgcttgc cgacaccgta acttttggcg
      721 ttttacctgc cttaatgctg tatcattatt ttaaaattgg catggctgtg catggtgtaa
      781 tacaaccggc atggctgccc tatattgcat tattattggc tgtatttagt gccttgcgct
      841 tggcaaaatt taataacgac agccgtcaaa ccacatcgtt tattggcgta ccaacacctg
      901 ctatatcatt gtttattggc tcatggcctt tgtttatgca aaatccggcc atgcaaaatt
      961 taaacggaac aaatgcctgg cagtacttgc aacaaatgga tgttgcacaa ccggcatggc
     1021 aaaatatgct attgcatcct tggtttttat taccgttaac cttgctgatg tgtttcttat
     1081 tggtttgcga gcttccattg tttgcactaa aatttaaatc gtttacctgg gctgagaata
     1141 aacaacgata tatgttttta acaggtagtg ctatattgct acttattttc aaatttgcag
     1201 ccattccaat aattatcacc gtatatgtat tgctttcttt agtgatgcat gcaacggcaa
     1261 aacaaaaaag ttaagtatga tatttagtgc ttccattcag gtaatgccgc ttaaagcatt
     1321 gttagaccca cagggcaaag ccgtttcggc cagtatgaaa aatttaaatt tatcggctat
     1381 cgaaaacgta cgcataggta aaaatattac cctgcaagtt actgccgata ccgaaaccga
     1441 agcgcttaat atggttaccg aagcctgtaa aaaacttttg gccaatccca ttatggagga
     1501 gtttgaaatt tcaattaaag cactttaaca tctatttgct ttcgaacaac aatagtcccc
     1561 ctttaattac cgcccaattg attgctcaaa aaaccgttgt taagttttgc gttttattgc
     1621 tgtgttgcat tactacaggg tgtgcaacga tgttagatgg ccgttacagt aaaattatta
     1681 tttccgaagg caaacctgcc ggtgccagcg tgtattataa ggatgctttt attggatatg
     1741 caccgtgtgc catttatccc aaaaaacgtg acttgaaaaa acatactttg gtg
//
LOCUS       DAOMAA010000462         1786 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_70436_length_1786_cov_2.673021, whole genome shotgun sequence.
ACCESSION   DAOMAA010000462 DAOMAA010000000
VERSION     DAOMAA010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70436_length_1786_cov_2.673021"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /gene="lipB"
                     /locus_tag="PLO59_06680"
     CDS             <1..555
                     /gene="lipB"
                     /locus_tag="PLO59_06680"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013664007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HQV00828.1"
                     /translation="PHVYTLGKSGSEKNLLITADELAQHQATFFRINRGGDITYHGPG
                     QCVGYPILDLDLFFNDVHKYMRYLEEVIIRTIAHYGIEGIRIPGLTGVWLAATPTKPE
                     RKICAFGVRLSRWVTMHGWALNVNNDLSFFNHIVPCGISDKAVTSIQQELGYAVEISE
                     TKQLLKLNFAHVFEVETIDAQLAF"
     gene            661..1782
                     /locus_tag="PLO59_06685"
     CDS             661..1782
                     /locus_tag="PLO59_06685"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HQV00829.1"
                     /translation="MKKWIALALVIILFGALGFTGNLYLVKALYYNFANIDDYKIFEN
                     TVIKKNTGRPWPHAYSYKTQTQNKLFNDTWQQYETTAFIVIQNDSLVFEQYAQDVNQQ
                     TISGSFSVAKSIVSVLIGVALNEGAIKNINEPVANYLPAFNNKSKKNITLKHLLTMSS
                     GLKWDEAYSSLFSVTTKAYYGYDVATLVGNLPIETQPGINFNYQSCNTQLLGMILIKA
                     TGKTISQYAQEKLWQPLQAEHDALWSLDNSDGIEKCFCCFNSTATDFARIGALYLHQG
                     NWHGLQIVDSTFVKESIKPAHLLYQGKANTIYGLHWWLATVAGKPVYFARGILGQYIF
                     VLPHKNLVAVRLGNKRGEVLPDGYVSDINTYLNWLDKNY"
BASE COUNT          581 a          339 c          355 g          511 t
ORIGIN      
        1 ccgcatgttt acacattagg taaaagcgga agcgaaaaaa atttactcat aacagccgat
       61 gaattggcgc aacatcaggc aacgtttttt cgcatcaacc gtggaggcga tattacctat
      121 catggaccgg ggcaatgtgt gggctatcct attttagacc ttgatttgtt ttttaatgat
      181 gtgcataaat acatgcgcta ccttgaagaa gtaattatac gaaccatagc ccactatggc
      241 attgaaggca tacgcatacc gggccttacc ggtgtttggc ttgcggccac accaaccaaa
      301 cccgagcgca aaatttgtgc ttttggcgtg cgtttaagtc gctgggtaac catgcatggc
      361 tgggcattaa atgtaaataa cgatttaagt ttttttaatc atatagtacc atgtggcatt
      421 agcgataagg ccgtaacatc tatacaacaa gagctgggct atgcagtaga aatcagcgaa
      481 acaaaacagt tgctaaaact aaactttgcc catgtgtttg aggttgaaac tatagatgca
      541 caattggctt tttaggtaac taatacttgc cgctaaattt aaaagacaga taaaaaaaca
      601 acgtggcaat tgcagtaaaa acatagtatg tttgtttcat atcaagcatt tgaataacgt
      661 atgaaaaaat ggattgcctt agccttagtt attattctgt ttggtgcatt ggggtttaca
      721 ggcaaccttt atttggttaa agcattgtat tacaactttg ccaatattga cgactataaa
      781 atttttgaaa acactgtaat taaaaaaaat accggccggc catggccaca tgcatacagc
      841 tataaaaccc aaacccaaaa caagctgttt aacgatacct ggcagcaata cgaaactact
      901 gcgtttattg tgatacaaaa cgactcactt gtttttgaac aatatgcaca agacgtaaac
      961 caacaaacca tttcgggctc gtttagtgta gcaaaaagta ttgtaagcgt cttaataggt
     1021 gtagccttaa acgaaggcgc tatcaaaaac attaacgaac ctgttgccaa ttacttaccg
     1081 gcatttaata acaaaagcaa aaaaaacatc accctaaaac acttgcttac catgagcagt
     1141 ggcttaaaat gggatgaagc atacagcagc ttgttttcgg ttactaccaa agcttattat
     1201 gggtatgatg tagctaccct ggttggtaat ttgcccatag aaacccagcc cggtataaac
     1261 tttaattacc aaagttgtaa tacacaattg ttgggaatga ttttaataaa agccaccggc
     1321 aaaaccataa gccaatatgc gcaagaaaaa ctttggcaac cattacaagc cgaacatgat
     1381 gccctttgga gtttagataa tagcgatggt attgaaaaat gcttttgctg ttttaatagc
     1441 acagccaccg attttgctcg tattggcgcc ttgtacctgc atcagggcaa ttggcatggc
     1501 ctgcagattg ttgatagcac ttttgtaaag gaaagtataa agcctgccca tttattgtat
     1561 caaggcaaag ccaataccat ttatggttta cactggtggt tggcaactgt agccggcaag
     1621 cctgtttatt ttgcgcgcgg tattttagga caatatattt ttgtattacc acataaaaat
     1681 ttagtagccg tacgtttagg taataaacgt ggcgaagtgt taccggatgg ctatgttagc
     1741 gacattaata cttatttgaa ttggcttgat aaaaactatt aaaaca
//
LOCUS       DAOMAA010000463         1786 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_70455_length_1786_cov_2.144425, whole genome shotgun sequence.
ACCESSION   DAOMAA010000463 DAOMAA010000000
VERSION     DAOMAA010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70455_length_1786_cov_2.144425"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="PLO59_06690"
     CDS             complement(<1..533)
                     /locus_tag="PLO59_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008506234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HQV00830.1"
                     /translation="MHTTVDDAKALGLLPEEFDKITEILGRNPNFTELSIYSVMWSEH
                     CSYKNSITWLKTLPKDGPHMLAKAGEENAGLVAIDDETGCAFKIESHNHPSAIEPYQG
                     AATGVGGINRDIFTMGARPIAQLNSLRFGNINSDRTKWLVKGVVKGIGDYGNAFGIPT
                     VGGEVYFDDCYNINILV"
     gene            complement(642..1109)
                     /locus_tag="PLO59_06695"
     CDS             complement(642..1109)
                     /locus_tag="PLO59_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQV00831.1"
                     /translation="MEDLSIIKVLYVDDEAHNLNAFKASFRRVFTVFIALSADEAKQI
                     MSENEIHVLITDQRMPGTPGTELLAYAVKDKPDQIRILLTGFSDMDALQDAVNRGHIY
                     RYLQKPWDDEILRQTIIEAYQIYALKLEREALTQKLQQNNEQLEFYLRHKLLS"
     gene            1317..>1786
                     /locus_tag="PLO59_06700"
     CDS             1317..>1786
                     /locus_tag="PLO59_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014774133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00832.1"
                     /translation="MSLHISNHLDINMQSAFNNYKPIIFSCASDEKEIEQITTQNQVE
                     KFDTLFEQLKEFIKTCNPSQKFADAILKQKAEAYIHPFTMNNYGMWIYYPWSKRLVRT
                     LPEAEFIKVRTSRNQYKITPEEQLILQKKKIGIIGLSVGQSVAVTIAMERISNH"
BASE COUNT          602 a          337 c          302 g          545 t
ORIGIN      
        1 tttaccaaga tgttgatgtt atagcaatcg tcaaagtaaa cttcgccacc aacggtgggt
       61 atgccaaagg cattgccata atcgccaatt cctttaacta cacctttaac caaccattta
      121 gtacggtcgg agttaatatt accaaaacgc agtgagttta attgtgcaat gggtcgtgca
      181 cccattgtaa atatatcgcg attaatgcca cccacaccgg tagcagcacc ttgatagggt
      241 tcgatagccg aaggatgatt atgtgactca attttaaatg cgcaaccggt ttcatcatca
      301 atggcaacta agcccgcatt ctcttcgcct gctttggcta acatgtgagg cccgtctttg
      361 ggtaaagttt ttagccaggt aattgaattt ttatatgagc aatgctcgct ccacattact
      421 gaataaatgc ttagctcggt aaaattagga ttgcggccta aaatttcagt tattttatcg
      481 aactcttcgg gtaataagcc taatgctttg gcgtcatcaa cagttgtgtg catacaatct
      541 tatttatttt gagctgccaa aagtaatttt atttcaaaaa aggataatac ctgttttgct
      601 tatttacaca caataattgg ctatttaagc ctaaaatttt actaagacaa taatttatgt
      661 cttaaataaa attcaagttg ctcgttgttt tgttgtagct tttgagttaa tgcttcgcgt
      721 tccagtttta aggcataaat ctggtaggct tcaataatgg tttgacgcaa aatttcatca
      781 tcccagggtt tttgtaagta gcgataaatg tggccgcgat ttacggcatc ttgtaaggca
      841 tccatatcgc taaaaccggt aagtaaaatc cgtatctggt cgggcttatc ttttacggca
      901 taggctaata attcagtacc cggtgtacca ggcatacgtt gatcggtaat taaaacgtgt
      961 atctcatttt cggacattat ttgcttggct tcatcggccg aaagtgcaat aaacacagta
     1021 aatacacgcc taaaacttgc tttaaacgca tttaaattat gcgcttcgtc atcaacataa
     1081 agaaccttaa ttatcgacaa atcttccata ctaaaaagtg tattaattac agcttatgta
     1141 acatttgcaa attgaaaact aacagaacaa ataatcaaac cctgcaggca atcaaaaaga
     1201 aactgaaaaa aattaaaatt gagaatcaaa ataacaccaa actatcaaaa tgataagttg
     1261 ctacaaaatt ataaaattaa actaaacttg cctgctcatt cccctatttt taattcatga
     1321 gtttacacat ttccaatcac cttgacataa acatgcaaag tgcttttaac aattacaaac
     1381 caataatatt tagctgtgca tctgacgaaa aagaaattga acaaattaca acacagaatc
     1441 aagttgaaaa gtttgatact ttgtttgaac aattaaaaga atttataaaa acctgtaatc
     1501 cttcgcaaaa gtttgcagat gctattttaa agcaaaaagc cgaagcttac atccatccat
     1561 ttactatgaa taattatggt atgtggattt attatccctg gagtaaacgt ttggtaagaa
     1621 ccttgcccga agcagagttt attaaagtac gcaccagccg caatcaatat aaaattacac
     1681 cggaagaaca gcttatcctt cagaaaaaaa agataggtat tatcggctta tcagtaggtc
     1741 aatcggtagc ggttaccata gctatggagc gtataagtaa ccatat
//
LOCUS       DAOMAA010000464         1778 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_71023_length_1778_cov_2.872316, whole genome shotgun sequence.
ACCESSION   DAOMAA010000464 DAOMAA010000000
VERSION     DAOMAA010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71023_length_1778_cov_2.872316"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1383)
                     /locus_tag="PLO59_06705"
     CDS             complement(<1..1383)
                     /locus_tag="PLO59_06705"
                     /inference="COORDINATES: protein motif:HMM:NF012873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HQV00833.1"
                     /translation="MNLTPVDFDPFKDLDKIIPTSEPQREILGSVKFGNDAANCAYNE
                     SLTLKLTGPFVYDAIVKAANAVVARHGSLRATFIEEGVKMRIADNTTIDVPLIDISNI
                     ATAEQQAHLNEIITEDVSAPFDLPNGPLFDVKVIKLAAQLHYVIITAHHIVCDGWSMG
                     IVMQDLSAFYNQHVTGQPAVLQPAIQFDDYVADEIAFEASTAYETTAQYWLNLYKDYN
                     QVFELPTDKPRPALRSYNAKRIDCPLPNDVITQIKKAGAQLGCSLVNTLIAAYEVFLF
                     KLTKQPNIILGLPSAGQSVTDNYSLVGHCVNLLPLKTRVDGNTSFSDYLVARKKTLFD
                     AFENQRYTFGTLIKKLNVPRDPSRIPLVPVAFNADLGLADGVAFEKLQLELFSNPRNY
                     ENFEIFFNISGQQNNLAIECTFNTDLYDNDLMHNRLHEFVFVLEQLAADMKTKINNVQ
                     LTPINEVEIIK"
BASE COUNT          564 a          302 c          344 g          568 t
ORIGIN      
        1 tttaataatt tcaacttcgt taatgggtgt aagttgtacg ttattaattt ttgttttcat
       61 atcggcagca agttgttcca atacaaaaac aaattcatgc aaacggttat gcatcaaatc
      121 attgtcatac aaatcggtgt taaaagtaca ttcaatggcc aggttgtttt gttggcctga
      181 gatattgaaa aaaatttcga agttttcgta gttacgcgga ttcgaaaata actccagttg
      241 cagtttttcg aatgcaacac catcggccaa gcctaagtcg gcattaaatg caacaggtac
      301 taacggaata cgtgatgggt cgcgtggtac gtttagcttt tttataagtg taccaaatgt
      361 gtaacgttga ttttcgaatg catcgaacaa ggttttttta cgtgccacca ggtaatcgct
      421 aaaagaagta ttaccgtcaa cacgtgtttt aagtggcaat aaatttacgc aatgacctac
      481 taatgagtag ttatcggtaa cagattggcc ggcagatggc agacccaaaa taatgtttgg
      541 ctgtttggta agtttaaata aaaaaacttc atatgcggca ataagtgtat ttaccaaact
      601 gcaacccaat tgtgcaccag cttttttaat ttgtgtaata acatcattgg gtaaagggca
      661 gtcaatacgt ttggcattat aactgcgcaa agcgggccgc ggtttatcag ttggcaactc
      721 aaaaacctga ttataatctt tataaaggtt aagccaatat tgagcagttg tttcgtaagc
      781 agtacttgct tcgaatgcaa tttcatcggc tacgtaatca tcaaactgaa ttgccggttg
      841 caatacagca ggttgcccgg taacatgttg gttgtaaaag gcacttaaat cctgcataac
      901 tatacccata ctccaaccat cgcatacaat gtgatgcgct gtaataatta cataatgtaa
      961 ttgcgctgcc agctttataa ccttaacatc aaacaaaggg ccgttgggta aatcaaatgg
     1021 ggccgataca tcttcggtaa taatttcgtt taaatgtgct tgttgttcgg ctgtggcaat
     1081 attactgatg tcaattagtg gtacatctat agtagtatta tctgcaattc gcatttttac
     1141 accttcttcg ataaaagtag cacgcaatga gccatgacgt gcaactacag cattggccgc
     1201 ttttacaata gcatcgtaaa caaaagggcc ggtaagtttt aaggttagcg attcgttata
     1261 ggcgcaattg gcagcatcgt ttccaaattt tacggagcct aaaatttcgc gctgtggttc
     1321 ggaagtagga ataattttat caaggtcttt aaacgggtca aagtcaacag gagtaagatt
     1381 cattatttaa atatcagata tacaacaact taatttttaa atgcaacagg cttatgccaa
     1441 ggtgcaaata taggtcttta aggttattta tggcatagat gaacttgaac tatttttacg
     1501 ctaagacacg ctaattgaag catagagaaa gggtttttaa aaatatattt tggtttacat
     1561 tacttaatca gattacgata taaaagttta tttgcgcagc taaaatcctt tttctttttt
     1621 tgacgcaacc acgatttcga attttaaatt gtaaaaccat tacacacttt ataaaaccgg
     1681 caaatgttta attatataaa aaagttttat gaaaaaaggt tatctgttat ggttagcaat
     1741 ggtttatgca acgtgcagca ttgcgcaagt taattttt
//
LOCUS       DAOMAA010000465         1775 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_71271_length_1775_cov_2.695349, whole genome shotgun sequence.
ACCESSION   DAOMAA010000465 DAOMAA010000000
VERSION     DAOMAA010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71271_length_1775_cov_2.695349"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="PLO59_06710"
     CDS             complement(<1..328)
                     /locus_tag="PLO59_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HQV00834.1"
                     /translation="MEVDILAIGVHPDDVEISAAGTLLAHIAMGKTAGIVDLTRGELG
                     TRGTAQTRDAEAAEAAKILGVSFRKNLEMADGFFTNDETHVRKVIQVLRLHKPTIVLC
                     NAIADRH"
     gene            478..1176
                     /locus_tag="PLO59_06715"
     CDS             478..1176
                     /locus_tag="PLO59_06715"
                     /inference="COORDINATES: protein motif:HMM:NF015344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HQV00835.1"
                     /translation="MVCIFKQALHLAHMFFNQVITLIKKDFRLELRQKFSIAGMLLYV
                     LATLYICFLAFRQIIDPVTWNALFWVINIFAAINASAKSFIQQPEGEHLYQYLLVPPQ
                     AVIVAKIIFNAILLTVISLVTFFFYALFIGNLAGNIPLFIGCLVLGNIGLASVLTMVS
                     ALASRANNPAALMAILSFPLLFPLLLILLRLSKTIVDDLGMDTELKYFIMLGGMSVLS
                     LTLGYILFPYLWRD"
     gene            1226..>1775
                     /gene="ccsA"
                     /locus_tag="PLO59_06720"
     CDS             1226..>1775
                     /gene="ccsA"
                     /locus_tag="PLO59_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014200578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HQV00836.1"
                     /translation="MSNILQKKWWKITGLVLVFYAIVGGMLLPVPARDILNETIRNLY
                     YHVTMWFTMIVLLTISVIYAIKYLSNFNINYDIIAREINKVAMLFALLGLFTGMLWAK
                     FTWGAYWTSDPKLNGVAASLLVYAAYFLLRNSLDDSEKRARFAAVYNIFAYVMMLVFI
                     LIMPRLTASLHPGNGGNPAFASY"
BASE COUNT          477 a          328 c          352 g          618 t
ORIGIN      
        1 ggtgcctgtc ggcaatggca ttacacaaaa caatggtagg cttatggagg cgcaaaacct
       61 gaataacctt gcgtacatgt gtttcgtcat tggtaaaaaa gccatcggcc atttctaagt
      121 tctttctaaa acttacgccc aatattttgg cggcttcagc tgcttcggca tcgcgtgttt
      181 gggccgtgcc gcgggttccc aattcgccac gggttaggtc aactatacct gctgttttac
      241 ccattgctat gtgtgccaac aacgtaccgg cagccgaaat ttcgacatcg tcaggatgta
      301 caccaattgc taaaatatct acttccattt tttatttatt gaaattttaa atgcctacct
      361 acccattact tataccggta agtaagttat ttattgcaca agtgtatgta aagcaaattt
      421 gagttgtaaa tggcattagt taattattat aaaaaaggct aaaaccaaac ttaaatcatg
      481 gtgtgcattt ttaagcaagc attacatttg gcgcacatgt ttttcaatca ggtaattaca
      541 ctcattaaaa aggatttcag gttagaactg cgtcaaaaat tttcgatagc cggtatgctg
      601 ctatatgttt tggcaacctt gtatatatgt tttttagcat tccgtcaaat tattgatccc
      661 gttacctgga atgccttgtt ttgggttata aatatttttg cagccattaa tgcatcggct
      721 aaaagtttta tacaacaacc cgaaggcgaa catttgtatc agtatttatt ggtgccgcca
      781 caagcagtaa ttgttgccaa aattattttt aatgccatac tgctaaccgt tatttctttg
      841 gtaacatttt tcttttacgc tttgtttata ggtaaccttg caggcaacat acctttgttt
      901 attggctgtc tggtgttggg caatattggc ttggcatcgg ttttaactat ggttagtgca
      961 ttggcatcgc gtgctaataa tccggcagct ttaatggcta tacttagttt tccgttactg
     1021 tttccgttgc tgttgatttt attgcgttta tcaaaaacta ttgttgatga tttgggtatg
     1081 gatacagagc taaaatactt tataatgctt ggtggtatga gtgtactgtc gctaacctta
     1141 ggctatattt tatttccata cctttggcgc gattagcgtt ttaaacaacg catcgaacca
     1201 gaaaaacctc agctattaaa atcaaatgag caacatactt cagaaaaaat ggtggaaaat
     1261 tacaggcttg gttttggtgt tttacgccat tgttggtggt atgttattgc cggtacctgc
     1321 acgcgatatt ttaaacgaaa ccatcagaaa cttatactat catgtaacca tgtggtttac
     1381 tatgattgtt ttgcttacaa tttcagtgat ttatgccatt aagtatttga gcaattttaa
     1441 tataaactac gatataatag cccgcgaaat aaataaagta gccatgctgt ttgccttatt
     1501 aggtttgttt acaggcatgt tgtgggcaaa atttacctgg ggcgcttatt ggaccagcga
     1561 ccccaaactt aatggtgttg ctgcatcgct gttagtttat gccgcatact ttttattgcg
     1621 caattcttta gatgatagtg aaaaacgtgc gcgttttgct gccgtgtata acatttttgc
     1681 ttatgtaatg atgttggttt ttattttgat aatgccacgc ttaacagcca gcttacatcc
     1741 gggcaatggt ggcaatccgg catttgcatc ctatg
//
LOCUS       DAOMAA010000466         1775 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_71286_length_1775_cov_2.325581, whole genome shotgun sequence.
ACCESSION   DAOMAA010000466 DAOMAA010000000
VERSION     DAOMAA010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71286_length_1775_cov_2.325581"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1577
                     /locus_tag="PLO59_06725"
     CDS             <1..1577
                     /locus_tag="PLO59_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HQV00837.1"
                     /translation="FGGEVERVLSMADGVLLLVDAFEGPMPQTRFVLQKALSQGLKPI
                     LVINKVDKENCRPDEVHESVFDLMFNLDATEEQLNFPTLYGSSKQGWMSTDVTKPTTD
                     ITALLDAILEHIPAAPVREGTPQLLITSLDYSSFVGRIAIGRIHRGTLKEGLPVMLCK
                     RDGTKVKSKVKELMTFEGLGKRKVAEVNAGDICAIVGLDNFEIGDTVADIENPEPLKS
                     VAIDEPTMNMLFTINNSPFFGKEGKFVTSRHLRDRLYKEIEKNLALRVEETDSPDSYL
                     VFGRGILHLSVLIETMRREGYEFQVGQPQVIIKHIDGVKCEPFEILSIDAPEEVAGKA
                     IELATMRKGELLIMEPKGDLQHLEFEIPARGLIGLRNAVLTATQGKAIMNHRFKEYRP
                     WKGEIPSRNNGVLIAMDSGKATPYAVDMMQDRGRFIIDSTADVYAGQIVGEHIKPGDL
                     VINVTRTKKLTNMRASGSDDNVRITPKLDFSLEENLEYIEADEMVEITPQTIRLRKLH
                     LNHEDRKRHEKKMQAV"
BASE COUNT          546 a          328 c          383 g          518 t
ORIGIN      
        1 attttggtgg cgaggttgag cgcgtactaa gtatggccga tggtgtattg ttattggttg
       61 atgcctttga aggccccatg ccacaaaccc gttttgtgct gcaaaaagca ttatcgcaag
      121 gcttaaagcc catattggta attaataaag ttgataaaga aaattgccgt cctgacgaag
      181 tacatgaatc ggttttcgac ttgatgttta atttagatgc aacggaagag caactcaact
      241 ttccaacgct ttatggctcg tcaaaacaag gttggatgag caccgatgta acaaaaccta
      301 caactgatat tactgcattg ttagatgcaa tattagagca tatacctgct gcaccggtac
      361 gcgagggtac accccaatta ttaattactt ctttagatta ctcatcattt gttggccgta
      421 ttgccatcgg gcgtattcac cgtggcactt taaaggaagg attgccggta atgttgtgta
      481 aacgcgatgg cactaaagtt aaaagcaagg taaaggagct aatgactttt gaaggattag
      541 gtaaacgcaa agtagccgaa gtaaatgccg gtgatatttg tgccattgtg ggcttagata
      601 atttcgaaat tggtgatacc gttgccgata ttgaaaaccc cgaaccactt aaaagtgtgg
      661 ctattgacga gcctaccatg aatatgttgt ttactataaa caactcaccc ttttttggta
      721 aagaaggtaa atttgttacc tcacgccatt tacgcgaccg cttatataaa gagattgaaa
      781 aaaacttggc attgcgcgtt gaagaaaccg actcgcccga ctcttactta gtatttggcc
      841 gtggtatatt acacctgtcg gttttaattg aaaccatgcg tagagaaggt tatgagtttc
      901 aggtaggtca accacaggtt attatcaaac atattgatgg cgttaaatgt gagccattcg
      961 aaattttaag tattgacgca cccgaggaag ttgcgggtaa agccattgaa ttggcaacta
     1021 tgcgtaaagg cgaactttta ataatggaac ctaaaggcga tttgcaacat ttggaatttg
     1081 aaatacctgc acgtggctta attggattgc gaaatgccgt tttaaccgca acacaaggca
     1141 aggctattat gaatcataga tttaaggagt atcgtccctg gaaaggcgaa attccatcgc
     1201 gtaacaatgg tgttttaatt gctatggaca gtggcaaagc aacaccttat gctgtagata
     1261 tgatgcaaga tcgtggtcgt tttattattg attcaactgc cgatgtgtat gccggacaaa
     1321 ttgttggcga gcatattaaa ccgggcgatt tggtaataaa cgtaacccgc accaagaaac
     1381 ttacaaatat gcgtgcttcg ggtagtgatg ataatgtgcg tataacgcct aagttagatt
     1441 tttcgctcga agaaaatctg gagtacatag aagccgatga aatggttgaa ataacaccac
     1501 aaaccatcag gttgcgtaaa ttacacctca accacgagga ccgtaaacgc cacgaaaaga
     1561 aaatgcaagc agtatagttt ttacctaagc tttttacaaa atattaaacc ggccctaagc
     1621 cggttttttt atagatgcaa ttattcaatt tttatatttt gcatcccttt ccattacata
     1681 caaaccatgt ttttaaaaat atatcaccat aaggtgtttc gctattttat tgccgcaggg
     1741 gtagcaacct tagttgatat cagtatgtac tttat
//
LOCUS       DAOMAA010000467         1772 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_71484_length_1772_cov_3.132790, whole genome shotgun sequence.
ACCESSION   DAOMAA010000467 DAOMAA010000000
VERSION     DAOMAA010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71484_length_1772_cov_3.132790"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1150
                     /locus_tag="PLO59_06730"
     CDS             <1..1150
                     /locus_tag="PLO59_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HQV00838.1"
                     /translation="QFLINQNITFLNFGSFGACVKPVFNELIRLQTLLEFEPVQFITV
                     NGPRYLAASRKALAQFINCNMDDVVYVPNPSFAVNLVAKNLKLKAGDEILTTNLEYGA
                     CDKTWEFYCNEWGAKYVRQPVTLPLTDTQTFVNDFFKGLTPRTRLIFISHITSTTALK
                     FPVDEICAKAKEMGLLTFVDGAHAPGHVAINLGLLKADFYTGACHKWMMTPKGSSFLY
                     ADKKLQPMLNPLIVSWGYNSNMASNSTFMDYHQFNGTRDFSAYLTIPTAITFMQQNNW
                     DEVGMHCHKLVVENATRFCALFETEPLSPLNNNFIGQMCSIPVNTNHPVELQQHLFNA
                     FKIEVPVMCIDGKTYIRYSIQAFNTQSDLDKLYDALATIKQKGKLLLQ"
     gene            complement(1185..>1772)
                     /locus_tag="PLO59_06735"
     CDS             complement(1185..>1772)
                     /locus_tag="PLO59_06735"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HQV00839.1"
                     /translation="MIRVGANYQVKPNILLRTGYAFAETYNYGDIPINGLGRDFSEHR
                     IFEMVQLTHKEGRFDLIHRYMLEQRFVGRYSSADVSKEDEFPMVNRIRYMLRIQVPLK
                     GNAIVDKTPYAAVYDEVMIAFGKNVNANVFDQNRIGLLLGYRFNKTIRVETGFINQIA
                     QFGRLVNGKNVFQHNSGFMVNAIFNFDVSKKAKLQ"
BASE COUNT          548 a          357 c          317 g          550 t
ORIGIN      
        1 tcaattttta attaaccaaa atattacctt tttaaacttt ggttcgtttg gtgcttgcgt
       61 taaaccggtg tttaacgaac taattcggct gcaaacttta ctcgagtttg aaccggttca
      121 gtttataacc gtaaacgggc cacgatatct tgcagcatca cgtaaggcat tagcacagtt
      181 tattaattgc aatatggatg atgtagttta tgtgcccaat ccatcgtttg ccgttaacct
      241 ggtggctaaa aatttaaaat taaaagcagg tgatgaaata ttaaccacaa atttagaata
      301 tggtgcctgc gataaaacct gggaatttta ttgcaatgag tggggtgcaa aatatgtgcg
      361 gcaacctgtt accttaccac ttacggatac acaaaccttt gtaaacgatt tttttaaagg
      421 gctcacaccg cgcacacgtt taatatttat cagccatata accagcacaa cggctttaaa
      481 atttccggtt gatgaaattt gtgccaaagc caaagaaatg ggcttactca cttttgttga
      541 tggagcccat gcgcccgggc atgtagccat aaaccttggt ttactgaaag ccgattttta
      601 taccggtgcc tgccacaagt ggatgatgac acccaagggc agctcgtttt tatatgctga
      661 taaaaaatta cagcctatgc tcaatccttt gattgttagc tggggctata acagcaacat
      721 ggcaagcaac agtacgttta tggattacca tcagtttaac ggcacgcgtg attttagcgc
      781 ctacttaacc attccaacag ccattacttt tatgcaacaa aataattggg atgaagtagg
      841 tatgcattgc cataaattgg tggttgaaaa tgcaacacgg ttttgcgcgc ttttcgaaac
      901 cgaaccttta agtcctttaa ataataattt tattggtcag atgtgtagca tacctgttaa
      961 taccaatcat cctgttgaac tgcaacagca tttgtttaat gcttttaaaa ttgaagtacc
     1021 ggtaatgtgt attgacggga aaacatatat caggtatagc attcaggcat ttaatacaca
     1081 aagcgatttg gataaactgt atgatgcttt agcaaccatc aaacaaaaag gcaaactttt
     1141 acttcagtaa tatttctgat tttaaaagtg catcacgttg ttacttattg cagcttggcc
     1201 tttttactaa catcaaaatt aaaaattgca ttaaccataa aaccgctatt gtgttgaaac
     1261 acatttttgc catttaccaa acgaccaaac tgtgcaattt gatttataaa tccggtttct
     1321 actctgatgg ttttattaaa gcggtagcct aacaacagcc ctatacgatt ttgatcaaaa
     1381 acatttgcat ttacattttt gccaaatgct atcatcacct catcgtaaac ggcagcatat
     1441 ggtgttttgt caacaatggc attgcctttt aatggcacct gaatacgcaa catatatcgg
     1501 atgcggttaa ccatcggaaa ctcatcttct ttactcacat cagccgaact gtaacggcca
     1561 acaaatcgct gctccaacat atagcgatgt attaaatcaa acctaccttc tttatgtgta
     1621 agctgtacca tttcgaaaat gcgatgttct gaaaaatctc tacctaaacc gttaatcgga
     1681 atatcgccat agttatatgt ttcggcaaag gcataccctg tacgcaaaag tatattgggt
     1741 ttaacctgat agttagcgcc cactctaatc at
//
LOCUS       DAOMAA010000468         1769 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_71699_length_1769_cov_3.214702, whole genome shotgun sequence.
ACCESSION   DAOMAA010000468 DAOMAA010000000
VERSION     DAOMAA010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71699_length_1769_cov_3.214702"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..117
                     /locus_tag="PLO59_06740"
     CDS             <1..117
                     /locus_tag="PLO59_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009090556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase dimerization
                     domain-containing protein"
                     /protein_id="HQV00840.1"
                     /translation="KVTTPKGCTIAGLNEMEHQGFSSSLIKGISVSYQKIGR"
     gene            190..792
                     /locus_tag="PLO59_06745"
     CDS             190..792
                     /locus_tag="PLO59_06745"
                     /inference="COORDINATES: protein motif:HMM:NF025224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HQV00841.1"
                     /translation="MNFCRSLIIVCSILPLSILAQLDSTTLSIQKTYFSLSDALQQPD
                     SVYKLNISKKKIKTWPNEIYKFKNLQVLNISKNDLTTLPDSIYLLNNLQVLIASNNKL
                     TAIPANIGALKGLTHINFNRNLIVTIPVEIGNLQALEVLEMWDNELDTIPDEIKNLGN
                     LRILELRGILFSEDDQRYIKKLVPHAHVYFSPWCACANKF"
     gene            complement(789..>1769)
                     /locus_tag="PLO59_06750"
     CDS             complement(789..>1769)
                     /locus_tag="PLO59_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008240985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HQV00842.1"
                     /translation="KLFPTIPAGHASYGHMVMNFSANILGLDNAATPFGLKAMQSLQE
                     INPAKEKASDAQIMFLALHASGLTLIPVSIIAVRAANGSHNPNEVFIPLILSTFVCTI
                     TALVVTSIKQRINLLQAKLFLPLAAIFSLLAIFVWYLKTLPPHALSEFSDAFSNGAIL
                     AVIALIVFGGLYKRINLFDAFVDGAKGGFDTAVRIIPYMVGMLVAVSMLRTSGAFDLI
                     IIPIKNAIAASGMDTQWAEGLPVALLRPFSAGGARGFMIDAMRTYGADSFVGKLVSVL
                     QGSSETTFYVVALYFGSVSVKDTRYAIPVMLLADLAGVLFSIFICYYLFA"
BASE COUNT          604 a          356 c          327 g          482 t
ORIGIN      
        1 aaagttacta cgcctaaagg ctgtaccatt gccggcctta acgaaatgga acatcagggc
       61 tttagttctt cgttgataaa aggtataagt gttagctatc agaaaatagg cagataaata
      121 cttcgggttt tgcaactaaa ttcaaacaac tgcaacatat aaccatacat tattcgtttt
      181 tattgtacca tgaatttttg caggagctta attatcgtat gtagtatatt gccgctaagt
      241 atattggcgc agttagatag tacaacccta tccatacaaa aaacttattt cagtttatca
      301 gatgcgctgc aacaacccga ctcggtttac aagctcaaca tcagtaagaa aaaaataaaa
      361 acatggccca atgaaattta caagtttaaa aacttgcaag tgcttaacat cagtaaaaat
      421 gatttaacta cactacccga ttccatttac ctgctaaaca acttgcaggt attaatcgca
      481 tcaaacaata aattaacagc cataccggct aatataggtg cgctgaaagg attaacacat
      541 ataaatttca atcgcaatct gatagtaacc ataccggttg aaataggaaa cctacaagca
      601 ttggaagtat tagaaatgtg ggataatgaa ttagacacta ttcccgatga aattaaaaat
      661 cttggcaacc tgcgtatatt ggagttgcgc ggtattttgt ttagtgaaga cgatcaacgt
      721 tacattaaaa aactggtgcc ccatgcacat gtttattttt cgccctggtg tgcttgtgcc
      781 aataagtttt aagcaaataa gtagtagcaa ataaaaattg aaaacaatac accggctaaa
      841 tcggcaagca acataaccgg tattgcatag cgcgtatctt taaccgatac cgaaccgaag
      901 tatagtgcaa caacataaaa tgttgtttca gacgaccctt gcaaaacaga aactaatttg
      961 cctacaaatg aatcggcacc ataggtgcgc attgcatcaa tcataaagcc acgcgcacca
     1021 ccggcactaa aaggccgcaa cagtgcaaca ggcaaaccct cggcccattg cgtatccatg
     1081 cccgatgcgg caatagcatt tttaatgggt ataataatta aatcaaatgc accactggta
     1141 cgcaacatgc taacagctac caacatacca accatataag gtatgatgcg cactgcagtg
     1201 tcgaaaccgc ctttggcgcc atcaacaaat gcatcaaata aattaatgcg tttatacaat
     1261 cctccaaaaa ctattaatgc tattacggcc aatattgcac cattgctaaa tgcatcgcta
     1321 aactcactaa gtgcatgtgg tggtaatgtt tttaaatacc aaacaaaaat ggcaagcaaa
     1381 ctaaatatgg cagctaatgg caaaaacaat tttgcttgta ataaattgat gcgttgtttt
     1441 attgaggtaa caactaatgc tgtgatggta caaacaaagg tgcttagtat gagcggaata
     1501 aaaacttcgt tggggttatg cgatccgttt gcagcgcgta cggcaattat tgaaaccgga
     1561 attaaagtaa gtcccgatgc atgcaatgcc aggaacatta tttgcgcatc gcttgctttt
     1621 tcttttgccg gatttatttc ctgtaacgat tgcatggcct ttaaaccaaa aggtgttgca
     1681 gcattatcta aacctaaaat attggcgcta aagttcataa ccatgtggcc gtatgatgca
     1741 tggcctgccg gtattgttgg aaacagttt
//
LOCUS       DAOMAA010000469         1768 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_71781_length_1768_cov_2.935201, whole genome shotgun sequence.
ACCESSION   DAOMAA010000469 DAOMAA010000000
VERSION     DAOMAA010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71781_length_1768_cov_2.935201"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /locus_tag="PLO59_06755"
     CDS             complement(<1..919)
                     /locus_tag="PLO59_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016198792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HQV00843.1"
                     /translation="MEKLIKVVCLILGGGAGSRLYPLTSIRSKPAVPIAGKYRLIDIP
                     ISNCLHSGIKRIFVLTQYNSASLNQHIKNTYHFDHFTYGFVDIIAAEQRPGSEKWFQG
                     TSDAVKQTLPHLQDHDFDYILILSGDQLYQMDLREFVNMHVESNAEISIATLPVNKND
                     APGFGIMKVDDNGIIQKFIEKPKADELVNWVSPVDEAQAALGNIYLASMGIYVFNRTT
                     LNRLFDENPHFTDFGKEIIPAAIKQGTKVAGYPYTGYWTDIGTIRSFFEANLDLAEII
                     PKFNLFDNNKLIYTRARMLPPSKFSHDTVL"
     gene            complement(926..>1768)
                     /locus_tag="PLO59_06760"
     CDS             complement(926..>1768)
                     /locus_tag="PLO59_06760"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQV00844.1"
                     /translation="GYLDWDEAINPLATGIKCSHKLTTVSRGYLDELKFGDNPMRWLF
                     NEYWQKSYGIVNGIDNEVWDPANDSNIYYPLQKDWTEFKQKNKAALCETVNFNPDLPL
                     MVFIGRLVKEKGGELLPQAIGQFVSQSKHMNFYILGSGVSHYEEQIKHISTIFNQNVT
                     NYIGYNEALAHKLYAAADFLIMPSLTEPCGLNQLYAMRYATVPVVRAVGGLKDTVIDY
                     GDADGYGIRFNNADAGDIIHSMHRAATLYHNNEQLNQIRQRISSFNYSWENAVKQYLE
                     IYNN"
BASE COUNT          521 a          343 c          331 g          573 t
ORIGIN      
        1 ttaaaaccgt atcgtgcgaa aattttgaag gtggcaacat gcgtgcacgg gtataaataa
       61 gtttattgtt atcaaataaa ttaaacttgg gtattatttc ggctaaatct aaattggctt
      121 caaaaaaact gcgtatagta cctatgtcag tccaataacc agtgtagggg taacctgcta
      181 ctttggtgcc ttgtttaata gctgccggaa ttatctcttt accaaaatcg gtaaagtgcg
      241 ggttttcgtc aaacaatcta ttaagcgtgg tacgattaaa cacataaatg cccatcgaag
      301 ccagatagat attgcccaat gctgcttgtg cttcatctac aggcgatacc cagttaacca
      361 attcatcggc tttgggtttt tctataaatt tttgaataat gccattatca tcaactttca
      421 taataccaaa acccggtgca tcgtttttat ttacaggtaa agtagctatg ctaatttctg
      481 cattactttc aacatgcatg tttacaaatt cacgcaaatc catttgatac aattggtcgc
      541 cactaagtat aagtatgtaa tcaaagtcat ggtcttgtaa gtgtggcaag gtttgcttta
      601 cggcatctga tgtgccctga aaccattttt cgctgccggg tcgttgttcg gcagcaataa
      661 tatccacaaa accatatgta aaatgatcga aatggtaggt attttttata tgttggttta
      721 acgaggccga gttatattgt gtaagcacaa aaatgcgctt gatacctgag tgcaaacaat
      781 ttgaaattgg gatatcaata agccggtatt taccggctat gggtacagca ggtttacttc
      841 tgatggatgt aagtggatat aaacgtgagc ctgcaccacc gcctaatata aggcatacta
      901 ctttaattaa tttttccata gctgcttaat tattataaat ttcgagatat tgtttaacgg
      961 cattttccca tgagtaatta aaggacgata tccgttgtct gatttgattt aactgctcgt
     1021 tattgtgata cagagtagca gcgcgatgca tactatgaat aatatcacct gcatcggcat
     1081 tattaaatcg gataccataa ccatcggcat cgccataatc aataacggtg tcttttaacc
     1141 cccctacagc acgtactaca ggcacagttg cataacgcat ggcatataac tgattgaggc
     1201 cacatggctc ggttaaactg ggcattatta aaaaatcggc agcggcataa agtttatgtg
     1261 ccagcgcttc gttatagcca atatagttgg ttacattttg attgaagatg gtacttatgt
     1321 gtttaatctg ttcttcgtag tggcttacac ctgaacctaa aatgtaaaag ttcatgtgtt
     1381 tgctttgtga aacaaattga ccgattgcct gcggtaataa ttcgccacct ttttctttta
     1441 ccaacctgcc aataaatacc atcaacggca aatcgggatt aaagtttaca gtttcgcaaa
     1501 gtgcggcttt gtttttttgt ttaaactctg tccaatcttt ttgaagcggg taataaatat
     1561 tgctgtcgtt tgccggatcc caaacttcat tatcaatgcc gtttacaata ccgtaacttt
     1621 tttgccaata ttcattaaac aaccatctca tgggattatc gccaaatttt aattcgtcta
     1681 aatacccacg gctaaccgta gttagtttgt gcgaacactt aataccggtg gctaatggat
     1741 ttatagcttc gtcccaatct aaataacc
//
LOCUS       DAOMAA010000470         1764 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_72137_length_1764_cov_2.397894, whole genome shotgun sequence.
ACCESSION   DAOMAA010000470 DAOMAA010000000
VERSION     DAOMAA010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72137_length_1764_cov_2.397894"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..484)
                     /locus_tag="PLO59_06765"
     CDS             complement(170..484)
                     /locus_tag="PLO59_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HQV00845.1"
                     /translation="MKEKTVQELQQMRNDGSDFQLIDVREEWEFETANINGELIPLGH
                     IMDEQDKISKSKPVIIHCRSGSRSTTAVKALEQLGYTNLYNLKGGIMAWKNEIDPTLD
                     VS"
     gene            complement(502..1257)
                     /locus_tag="PLO59_06770"
     CDS             complement(502..1257)
                     /locus_tag="PLO59_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019941827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HQV00846.1"
                     /translation="MNFELSNVPQRAVKPRQTGITMVMDKGLSVRQAEDLIESGLAYI
                     DIIKLGFGSALITPNLQQKLDVYRNAGVPVYFGGTMAESFIVRKKFDDYIRLLEKYKM
                     THAEISDGSITIPHDEKCEYIRRLAKQITVISEVGSKEEGIIIHPSVWTSMMLKELEA
                     GVWKVIAEARESGTVGIYRPNGKAHVMLINIITNKVPAEKIIWEAPQKSQQAYFIKHM
                     GADVNLGNIAPNEIIALESLRLGLRSDTFFQYL"
     gene            complement(1284..>1764)
                     /locus_tag="PLO59_06775"
     CDS             complement(1284..>1764)
                     /locus_tag="PLO59_06775"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQV00847.1"
                     /translation="IGMVLSYQEKAYEAIHYLKKSVELEPGNAEFWFALADCESTLGH
                     FNEAIACYENVIDLEPDNEDIYAEYALFLFEHNQIKDAIETIQMGIKTLPQNAEHYYY
                     AACFLYSDGKLQMAYEEFEQGLIKNFNDHKIIFEVLPPLQNDYRIIELISRYSDNQI"
BASE COUNT          489 a          329 c          316 g          630 t
ORIGIN      
        1 atcatttagt ttttaattgg gcgccaacga tgatcatttt aaaacaaatt ccaatacggc
       61 atttgacgta ttttatataa aaagaaagaa ttttaatact ttagtttaaa agcataactt
      121 agcagcagat taatagccat gagtaaatta agtaaaataa aaaagtaaat cagctcacgt
      181 caagcgttgg atctatttcg tttttccagg ccataatacc accttttaaa ttgtataaat
      241 tggtgtaacc taattgttct aaagctttaa cagcggtagt gcttcgcgag cccgaccggc
      301 agtgaataat aaccggtttc gatttcgaaa ttttatcttg ttcatccatt atatggccta
      361 agggtattaa ctcgccatta atgtttgcgg tttcaaactc ccattcttcg cgtacatcta
      421 ttaattgaaa atcgcttcca tcgtttcgca tttgttgcaa ctcttgtaca gttttttctt
      481 tcattttatt tggattaatt tttataggta ttgaaaaaaa gtatcgctgc gcaaacccag
      541 gcgtaaactt tccaatgcaa taatttcatt gggtgcaatg ttgcctaaat ttacatcagc
      601 gcccatgtgt ttaataaagt aagcttgttg tgatttttga ggtgcttccc aaataatttt
      661 ttcggccggt actttattgg taataatatt tataagcatt acatgtgctt ttccgtttgg
      721 gcggtatatg cccacggtgc cactttcgcg tgcttcggca attactttcc aaacaccagc
      781 ttcaagttct tttaacatca tactggtcca aacgctggga tgtatgatta tgccttcttc
      841 tttacttccc acttcgctaa ttacggtaat ttgctttgcc agcctgcgta tgtattcgca
      901 tttctcatca tgcggtatgg ttatagaacc atcgctgatt tcggcatgcg tcattttata
      961 tttttcgaga aggcggatgt agtcatcaaa ttttttccta acaataaatg attcggccat
     1021 ggtaccacca aaatatacgg gcacaccggc attgcgatat acgtctaatt tttgctgtag
     1081 gtttggtgtt attagtgcac tgccaaaacc taattttatg atatctatgt atgccagtcc
     1141 cgattcaatt agatcttcgg cttggcgtac gcttaaaccc ttgtccataa ccatggttat
     1201 gccggtttgg cgtggtttta cagcacgttg gggtacattt gataattcaa aattaatcat
     1261 gtaaaaagca ggttttggtt tgtttatatt tggttatcgc tatagcggct aattaattca
     1321 attatgcgat aatcattttg caaaggaggc aatacttcaa aaataatttt atggtcgtta
     1381 aagtttttta taagtccttg ttcaaactct tcataggcca tttgtaactt accatcacta
     1441 tataaaaagc aagcagcgta ataataatgt tcggcatttt gtggtagggt ttttattccc
     1501 atttgtatgg tttcaatggc gtcttttatt tggttgtgtt caaataaaaa cagggcatat
     1561 tcggcataaa tatcttcatt gtcaggttca aggtctatta cattttcata gcaggctata
     1621 gcttcgttaa agtgtcctaa ggtactttcg caatcggcta aagcaaacca aaactcagca
     1681 tttccgggtt ctagctcaac ggattttttt aagtaatgta ttgcctcata cgctttttcc
     1741 tgatagctaa gcaccatacc aatg
//
LOCUS       DAOMAA010000471         1762 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_72271_length_1762_cov_2.602812, whole genome shotgun sequence.
ACCESSION   DAOMAA010000471 DAOMAA010000000
VERSION     DAOMAA010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72271_length_1762_cov_2.602812"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="PLO59_06780"
     CDS             <1..654
                     /locus_tag="PLO59_06780"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV00848.1"
                     /translation="FCLNDSAVAQAQLLFDMPVTWLLNQTNINTGNSCIAAPNTDGLY
                     TLSMVAANSVCSVQKDTTINVWPLPIKPNITIAGSALSSSAIYSNMWYFNGSSLNDTM
                     QAITALATGWYMVAVTNSYGCKTFSDSVYISGMGINDFAAAGIQLYPNPTHNWLQVKA
                     ANLISSIVIADAMGRKYAMQQNQNHYNFDTSKWPAGLYSATILYQGKVFVNTFLIQH"
     gene            complement(651..>1762)
                     /locus_tag="PLO59_06785"
     CDS             complement(651..>1762)
                     /locus_tag="PLO59_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HQV00849.1"
                     /translation="LKNEIHIETSSGFDINIIRALIDEGKVNKDVFILCNGFKKETYT
                     QGIIDLINDDFTNVIPILDNMNELSAYEAELKKPYKVGLRIAADEEPNFEFYTSRLGI
                     RYKDIVPFVKDKILKSPNAELKLLHFFINNGIKDNAYYWSELSKGIDMYCHLKQICPS
                     LDSLDIGGGWPIRTNLNFDYDYDYMADEIINIIKTTCEKNGVKEPNIFTEFGSFTVGE
                     SGAILFSVIDQKTQNDKETWNMIDSSFITTLPDTWGINQKFILLAINHWDNEYQRVNL
                     GGLTCDSEDYYSFESQTNRVFMPRINPAEGPLYLGFFHTGAYQESLGGYGGIQHCLLP
                     APKHVLIDRNAEGEYTTRLFAKEQSAKSMLKILGY"
BASE COUNT          548 a          348 c          312 g          554 t
ORIGIN      
        1 ttttgcctta acgattcggc agtagcacaa gcacaactgt tgtttgatat gcctgttacc
       61 tggttattaa atcaaacaaa tataaataca ggtaacagtt gtattgctgc tccaaatacc
      121 gatggccttt atacactaag tatggttgct gctaacagtg tatgtagcgt gcaaaaagat
      181 actacaatta atgtgtggcc gctacccatt aaacccaata taacaattgc aggaagtgcg
      241 ttaagcagca gtgcaattta cagcaatatg tggtatttta atggaagcag tttgaacgat
      301 accatgcaag caattacagc attggctaca ggttggtata tggttgccgt taccaatagt
      361 tatggttgta aaacatttag cgactcagtt tatatatccg gtatgggcat caatgatttt
      421 gcagcggcag gtattcagtt atatcccaac cctacacaca attggttaca agttaaagct
      481 gcaaatttaa taagcagtat tgttattgcc gatgcaatgg gccgtaaata tgccatgcaa
      541 caaaatcaaa atcattataa cttcgacaca agtaaatggc cggcaggttt atattcagct
      601 acgatactgt atcagggtaa agtctttgta aacacattcc tcattcaaca ttaataacct
      661 aatatcttaa gcatactctt agcgctttgc tctttagcaa ataagcgcgt agtatattcg
      721 ccttcggcat tacggtcaat aagtacgtgt tttggtgcag gtaaaaggca gtgttgaatg
      781 ccaccataac cgcccagcga ttcctgatat gccccggtat gaaagaaacc taagtatagc
      841 ggcccttcgg ctgggtttat gcgtggcata aatacacggt ttgtttgcga ctcgaaactg
      901 tagtaatcct cgctatcaca agttaaaccg cccaggttta cacgctggta ctcattatcc
      961 caatgattaa tggccagcag aataaacttt tgattaatgc cccaggtatc tggcagggta
     1021 gtaataaacg atgaatcaat catattccag gtttccttat cattttgcgt tttctgatca
     1081 attaccgaaa ataaaattgc accactttcg ccaacagtaa aactgccaaa ctcggtaaaa
     1141 atgttaggtt ctttaacgcc atttttttcg caagtggttt taatgatatt tattatctca
     1201 tcggccatat aatcgtaatc gtaatcgaaa tttaaattag tacgtatggg ccacccgcca
     1261 ccaatatcca gactatctaa gctcggacaa atctgtttaa gatggcaata catatcaatg
     1321 cccttgctta actcactcca atagtaggca ttgtccttta tgccgttatt aataaaaaag
     1381 tgtaataact taagttcggc attagggctt tttagtattt tatctttaac aaatggcaca
     1441 atatctttat atctgattcc caaacgtgaa gtataaaact caaaattggg ttcttcatcg
     1501 gcagcaatac gaaggccaac tttatagggt ttcttcaatt cagcttcata agcactaagc
     1561 tcattcatgt tatctaaaat cggaattaca ttggtaaaat catcattaat taagtcaata
     1621 atcccttgcg tgtaagtttc ttttttgaag ccattacaaa gtataaaaac gtccttattt
     1681 acttttccct catcaatcaa agcccgtatt atgtttatat cgaagcctga tgatgtttca
     1741 atatgaattt catttttaag tg
//
LOCUS       DAOMAA010000472         1761 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_72313_length_1761_cov_3.791325, whole genome shotgun sequence.
ACCESSION   DAOMAA010000472 DAOMAA010000000
VERSION     DAOMAA010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72313_length_1761_cov_3.791325"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1336
                     /locus_tag="PLO59_06790"
     CDS             <1..1336
                     /locus_tag="PLO59_06790"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV00850.1"
                     /translation="SGITINTQPSDITICHLGSASFSVAATGAVSYQWQIRNSAGFYT
                     DLQTNSTYSGVNTPTLSIYLANGLLNGSKYRCLLSDCYASNAATLTVNGATMMFGSSG
                     YKTVCQGGTATFSVIAIGAGVTYQWQVDTGTGYTNIVNGGMYSGAQDSVLIITNVSIS
                     TLVFKVQCFLTLLCAPYVLVPQVGELHITGPSTTINMQPVATPGCLLQPAQIAIGASS
                     AGASSYSWQVNNGTGWQILQNDSIYSGVNNDTLTINKTNSGMMHNNYRCWVNGCAVSD
                     TCSLKSKAVGGGSLNITPPAFCVNQPPATLSGGLPANGYYTGNSVCNNIFYPALAGVG
                     QHTITYFYNDSNGCLQSANQLVTTGYCTVQGIDQTAAPQPLWYPNPSAKWVTLQTAVT
                     GKLKVYNLIGDSYYKGNYTGGTNIDVSQWPKGIYFFELRMPDKKYVSKVMVI"
BASE COUNT          532 a          339 c          366 g          524 t
ORIGIN      
        1 ttcgggtatt accataaaca cacaacccag cgatatcacc atttgtcatt taggttcggc
       61 aagctttagc gtggcagcaa caggtgcagt cagctaccaa tggcaaatac gtaacagtgc
      121 cggcttttat accgatttgc aaaccaatag tacttacagt ggtgttaaca cacctacgct
      181 gtcgatatac cttgccaatg gattgttaaa cggcagcaaa taccgttgct tattgtcaga
      241 ttgttatgcc agcaatgcag caacgctaac ggtaaatggc gcaaccatga tgtttggcag
      301 ctctggatat aaaacagtat gtcaaggcgg tacggctaca tttagtgtga tagcaatcgg
      361 tgcgggtgtt acctatcaat ggcaagttga taccggcacc ggttatacaa atattgttaa
      421 tggcggtatg tatagtggtg ctcaggactc tgtacttatt attaccaatg tttcgatatc
      481 aaccctggtg tttaaagttc aatgcttttt aaccctttta tgtgcaccat atgtactagt
      541 gccacaagtt ggcgaattac atattacagg cccttctacc actatcaata tgcagcccgt
      601 agcaacaccg ggatgtttgt tacagcctgc tcaaattgct attggtgcaa gcagtgcagg
      661 cgcttcaagt tatagttggc aagttaataa tggcaccggt tggcaaattt tacaaaacga
      721 cagtatttat agtggggtta acaatgatac attaaccatc aataaaacaa acagtggcat
      781 gatgcataac aactatcgct gttgggttaa tggttgtgct gtgtccgaca catgtagttt
      841 aaaatcgaaa gcagtgggag gcggttcttt aaacattaca ccaccggcat tttgtgtaaa
      901 ccagccaccg gcaactttaa gtggaggctt gcctgccaac gggtattaca ccggcaatag
      961 tgtttgcaat aatatttttt atccggcatt agccggagta ggtcaacaca ctattaccta
     1021 tttttataac gatagtaatg gttgtttgca atcggcaaat caattggtaa caacgggcta
     1081 ttgtaccgta caaggcattg atcaaacagc agcaccacag cctttatggt atccaaaccc
     1141 ctcagcaaaa tgggttacat tgcaaactgc tgttaccggc aaattaaaag tgtacaattt
     1201 aataggcgat agttattaca aaggcaatta tacgggaggt acaaatattg atgtaagcca
     1261 atggccaaaa ggcatttatt ttttcgaact gcgaatgcct gataaaaagt atgtaagtaa
     1321 ggttatggtt atataatttg ctgttatgca atcgcgagct ttactgaagc agatttgagt
     1381 tggcgtaaaa cttacgttac aatccaatta ctatgattag atatgcggct taaaattatg
     1441 gttgctatta atctgaaaac agtaatggga ttataatagc aaggtcttga ttaaatagcg
     1501 ttgtcgttat gttttaatcc tattcaatca tgcactaatt caatgctatg ttttaaaaac
     1561 caaaacactt ctgctgtgtt aatccctgta acagttaact caaccaatta aataatcgtt
     1621 taaaggatca aacaccatat caatccatcc gtttttgctg ttgtttaatt cttcatcatt
     1681 gggcaggtta ctatgggtta gcgagataag ggtgcctgcc tcgtggggtt taaaaagcaa
     1741 atttacaata gccggctgca t
//
LOCUS       DAOMAA010000473         1761 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_72315_length_1761_cov_3.561547, whole genome shotgun sequence.
ACCESSION   DAOMAA010000473 DAOMAA010000000
VERSION     DAOMAA010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72315_length_1761_cov_3.561547"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..940)
                     /locus_tag="PLO59_06795"
     CDS             complement(<1..940)
                     /locus_tag="PLO59_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009281321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HQV00851.1"
                     /translation="MINRRSFLKTIPVTALLIPQPLHKFYAKHLSTTEDDAFWENIAQ
                     AYNVDRSIINLNNGGVSPQPVAVQNRFVELNTEANKGPSYYMWRIMDKGREPLRARLA
                     NLAGCATDEIAIQRNTTEALGNVVMGINLQPGDEVVLSNMDYPNMLQAWKQRAQREGI
                     KLVFADLKLPSTDTDYLTNAYTRLFTSKTKVVHLTHMINWCGQLMPVAQIAAQANKFG
                     IEVILDAAHTFAHIPFSLSETGCHYAGTSLHKWLGAPFGTGFLYVAKDKIKNLWPLLP
                     SNEPQSNDIRKFEVLGTRSFAAEQGINAAIDFNESIG"
     gene            1079..>1761
                     /locus_tag="PLO59_06800"
     CDS             1079..>1761
                     /locus_tag="PLO59_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00852.1"
                     /translation="MKQFFTLTLKVLLLILIARDVQATHIMGGNLGYTYMGLQPNGEY
                     RYKVRLELYRSCAQGSSDLPISMRLGVYFENTANPTADKVQQDEYNNVPLISQQFISP
                     PAPNDTCSFTPSVCVEQGIYELEIDVPSSTMGYHLIMDRCCRNDVIANVFDPSNEGAA
                     WYAFIPATNIVNSSPTFAVPPVPFLCANDTNSILNAAFDPDGDVLTYNYVVPYGGLSS
                     PGQPNPNLPG"
BASE COUNT          493 a          372 c          344 g          552 t
ORIGIN      
        1 tacctatgct ttcgttaaaa tcaatagcag catttatgcc ttgttcggct gcaaaactac
       61 gggtgcctaa tacttcaaac ttgcgtatat cgttgctttg cggttcgtta ctgggcaata
      121 aaggccacag gtttttaatt ttatcttttg ccacatataa aaaccccgtg ccaaaaggcg
      181 cacccaacca tttgtgtaaa ctggtaccag cgtaatggca tcctgtttcc gataatgaga
      241 atggaatatg cgcaaaagta tgtgcggcat ctaaaataac ttcaattcca aatttgtttg
      301 cctgcgcagc aatttgtgct acaggcataa gctggccaca ccaattaatc atgtgtgtta
      361 ggtgtaccac ttttgttttt gatgtaaata accgtgtata agcatttgtc agataatcgg
      421 tatctgttga gggtaatttc aaatcagcaa aaaccagttt aataccttcg cgctgggcgc
      481 gttgtttcca agcctgcaac atatttggat agtccatatt tgaaagtacc acctcatcgc
      541 caggttgtaa atttatgccc attaccacat tacctaaagc ttcggtagta ttgcgctgta
      601 tggcaatttc atcggttgca caaccggcca agtttgccaa acgtgcgcgt aagggttcgc
      661 gccctttgtc cataatgcgc cacatatagt aactggggcc tttattggcc tcggtattaa
      721 gttcgacaaa acgattttga acagcaacag gttgaggaga tacaccacca ttattaaggt
      781 tgatgatact tctatccaca ttataagcct gggcaatgtt ttcccaaaat gcatcgtctt
      841 ccgttgtgga caagtgcttt gcataaaact tatgtaaagg ctgtggtatt aatagtgccg
      901 ttacaggtat tgtttttaaa aagcttcttc ggtttatcat ttttaaaaaa atttggggta
      961 ttcaaaatta cacaaatgca tatcaacaaa atgtaaactt tgtttgttaa atacggtcat
     1021 accaatatta atctacattt ggaggctttt tttaaacctc cactctttta ttgaaactat
     1081 gaaacaattt tttacgctca ccttaaaggt gctcttatta attttaatcg caagggatgt
     1141 gcaagccacg catattatgg gcggcaacct cggttatacc tatatgggat tacagcccaa
     1201 tggcgaatac cggtataaag tgcgtttgga actttaccgc tcctgtgcac aaggctcctc
     1261 tgatttacct atatctatgc ggttaggcgt ttattttgaa aacacagcta accctactgc
     1321 cgacaaagtg caacaagatg agtataacaa tgtacccctg atatcgcaac aatttattag
     1381 cccacctgct ccaaacgaca cctgctcatt tacacccagc gtttgtgttg aacaaggtat
     1441 ttatgagtta gaaattgatg tgccttcgtc aactatgggt tatcatttaa ttatggatag
     1501 gtgttgccgt aacgatgtaa ttgcaaacgt ttttgaccca agtaatgaag gtgctgcctg
     1561 gtatgctttt attcctgcta ccaatattgt aaacagttcg cctacgtttg cagtaccacc
     1621 ggtaccgttt ttatgtgcaa acgataccaa ctccatttta aatgcagctt ttgaccccga
     1681 tggtgatgtg ttaacttata actatgtggt gccatacggt ggcttatcaa gtccgggaca
     1741 acccaatcca aacttgccgg g
//
LOCUS       DAOMAA010000474         1761 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_72344_length_1761_cov_2.559203, whole genome shotgun sequence.
ACCESSION   DAOMAA010000474 DAOMAA010000000
VERSION     DAOMAA010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72344_length_1761_cov_2.559203"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PLO59_06805"
     CDS             complement(<1..231)
                     /locus_tag="PLO59_06805"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009231 - riboflavin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HQV00853.1"
                     /translation="MKQQLDSIEEAIKALQRGETIIVVDDEDRENEGDFVNAAANCTP
                     EVINFMATYGRGLICAPITETRCEELKLNMMVS"
     gene            353..1192
                     /locus_tag="PLO59_06810"
     CDS             353..1192
                     /locus_tag="PLO59_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002998450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HQV00854.1"
                     /translation="MKSKQHFNIGFSPCPNDTYIFDALIHSKIDSPFRFSPHLHDVQV
                     LNQMAMQQILPVTKLSFFAYAQVSAHYQILDAGSALGNGCGPLLISKNPNLDTKHNTL
                     NICVPGMQTTANLLLSLFYPQLSNKTPLLFSDIEQALVRNAFDAGVIIHENRFTYNRL
                     GLQLIADLGAMWELKTQLPIPLGCIAVARSLPEAVKKEISNLISSSISYANENVADVM
                     PYIKMNAQNMDEAVIKQHIDLYVNDYSVKLGSNGKTAINTLFAAGYDKQLIPELTQPI
                     FLN"
     gene            1203..>1761
                     /locus_tag="PLO59_06815"
     CDS             1203..>1761
                     /locus_tag="PLO59_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020605266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQV00855.1"
                     /translation="MIIVTGAAGFIGSCLIKNLLNKGMANIIAVDDFSNAEKQKNLVD
                     KPISKFIHRDVLTDFIKTHNQQIEFVFHIGARTDTTEFNVDVFNKLNLHYSQMVWQLC
                     VQYQIPMVYASSAATYGAGELGYDDNHEIINRLKPLNPYGDSKNDFDKWVLQQDKFPP
                     FWAGLKFFNVYGPNEYHKGRMASVIW"
BASE COUNT          562 a          306 c          309 g          584 t
ORIGIN      
        1 actaaccatc atgtttaatt ttaattcttc gcaacgtgtt tcagtaattg gtgcacaaat
       61 taatccacgg ccataagttg ccataaaatt tattacttca ggtgtacaat tggcagcagc
      121 atttacaaag tcgccttcgt tttctctgtc ttcatcgtca accacaatta tggtttcacc
      181 tctttgtaaa gctttaattg cctcttcaat gctatctaat tgttgtttca aaacttctgt
      241 atttaaaaat gcggccgcaa aagtaatttt tttatccgtc tgaaattgta aagcaggttt
      301 tgcgctaaaa tgttgtttta aaaacaaacg ttacttttga tgccctgtaa ttatgaaatc
      361 aaagcaacat tttaatatcg gattttcgcc ttgccctaac gatacctaca tatttgatgc
      421 attaattcat tcaaaaattg atagtccatt taggttttct ccgcatttgc atgatgtgca
      481 ggtattgaat caaatggcaa tgcaacagat tttgccggtt accaaattaa gtttttttgc
      541 ttatgcacaa gtaagtgccc attatcaaat attagatgcc ggtagtgctt tgggtaatgg
      601 atgtggcccc ttgctaattt ctaaaaatcc aaatttagat acaaagcata atacgcttaa
      661 tatatgtgta cccggcatgc aaacaacagc caatttactt cttagtttgt tttatccaca
      721 gctatcaaat aaaacaccgc ttttgttttc agatattgaa caagcgcttg tacgaaatgc
      781 ctttgatgcc ggagttataa tacatgaaaa ccgttttaca tataaccgat tgggcttgca
      841 attaattgcc gatttaggtg ccatgtggga gttaaaaacc cagttgccta tacctttagg
      901 ttgtattgcc gttgcccgta gtttacctga agcagtaaaa aaagaaataa gcaatttaat
      961 ttcatcaagt attagctatg caaatgaaaa tgtagccgat gttatgcctt atattaaaat
     1021 gaatgcgcag aatatggacg aagccgttat aaaacaacac attgatttgt atgtaaatga
     1081 ttactcggtt aaattgggca gcaatgggaa aacagcaata aatactttat ttgccgctgg
     1141 ttatgataaa caattgatac cagaattaac acaacctatc tttttaaatt aattaactaa
     1201 ttatgataat tgttaccgga gctgccggtt ttattggcag ttgtttaata aaaaatttat
     1261 taaataaagg catggcaaac attattgctg tagatgactt tagcaatgct gaaaagcaaa
     1321 agaatttagt tgataagcct attagtaaat ttatacaccg cgatgtcctt accgatttta
     1381 taaagaccca taatcaacaa attgaatttg tttttcatat aggcgcacgt accgatacta
     1441 ctgagtttaa tgtagatgtg tttaacaaat taaacttgca ctactcgcaa atggtatggc
     1501 agttatgcgt acaatatcaa attccgatgg tgtatgccag ttcggctgct acttatggtg
     1561 cgggtgagtt aggttatgac gacaatcacg aaataattaa ccggttaaaa cctttaaatc
     1621 cttatggcga ttctaaaaac gactttgata aatgggtact acaacaagat aagtttcctc
     1681 cgttttgggc aggccttaaa ttttttaatg tgtatggccc taacgaatac cataaagggc
     1741 gtatggcatc ggtaatatgg c
//
LOCUS       DAOMAA010000475         1761 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_72349_length_1761_cov_2.445487, whole genome shotgun sequence.
ACCESSION   DAOMAA010000475 DAOMAA010000000
VERSION     DAOMAA010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72349_length_1761_cov_2.445487"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1761)
                     /locus_tag="PLO59_06820"
     CDS             complement(<1..>1761)
                     /locus_tag="PLO59_06820"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HQV00856.1"
                     /translation="SFTKVTVSSPGNILGWDSNGGDSGGQGWYDLGFAASPTNANEVT
                     TGGVNVWRSTNGGSSFSLNGHWTGSGAPYVHADVHEIVYTDANTVWVACDGGLFKTVN
                     NGTTWTDLSDGLQISQMYGFGQSSTNASLFIQGWQDNGTNRCNGTTWQEVMGGDGMLA
                     FIDWNNDQNMWGSQYNGSLNRSTNGGNTWSSANGSINEAGAWVTPWLQDPITASTLYA
                     GFVNVWRSNNGGSTWTKISSFSNSGTLNAIAVSAANNQVLWAAKAGALYKTNNGGTNW
                     TTISNVPAGTISSIACSNTDPNRAWITYSGFSGGNKVFQTNDQGVTWINLSTSLPNIP
                     INYITRQNNSNDALYIGTDAGVYYKDSTLTIWQPFFNGLPNVIVTQLAIHYATGKIRA
                     STYGRGLWESDLYVPGAYAPTASFISDKNISCPGAAIQFTDVSAGQPTAWYWDLPGGN
                     PTSSAQQNPVVYYNTPGTYPVTLIAANAIGSHTYTVNSFINISSSATAAPATNNVTVC
                     GPTTATLTATGSGTGTLRWWDAPGGGNLVGTGPSLNINVNGSTAYYVDEDFPAGLIDY
                     TGETSNSIGAGAYFTANDIR"
BASE COUNT          466 a          418 c          389 g          488 t
ORIGIN      
        1 cgcgtatatc attagctgta aagtatgcac ctgcgccaat actgttgctg gtttcaccgg
       61 tatagtcaat taaaccggcc ggaaaatctt cgtcaacata ataagcagtg cttccgttta
      121 catttatatt taacgatgga cctgtgccta ctagattgcc cccaccgggt gcatcccacc
      181 agcgcaacgt acccgtgcca ctgcctgttg ctgttaaagt tgctgtagta gggccacaaa
      241 ccgttacatt atttgtagca ggagcagccg ttgccgaact gcttatatta ataaaactgt
      301 taaccgtgta ggtgtggctg ccaatggcat tagctgcaat tagtgttacc ggataggtgc
      361 cgggtgtatt gtagtaaaca accggatttt gttgtgccga tgaggtagga ttgccaccgg
      421 gcaaatccca ataccaggca gtgggttggc cggcactcac atcggtaaac tgtattgcag
      481 cgcccgggca tgaaatattt ttatctgaaa taaacgaagc tgtaggcgca taagcaccgg
      541 gtacatataa atcgctttcc cacaagccgc gtccataagt tgatgctcta attttaccgg
      601 ttgcataatg tatggccaat tgtgtaacaa taacatttgg caggccatta aaaaatggtt
      661 gccatatagt aagggtgcta tctttataat acacaccggc atcggtacct atatataatg
      721 catcgttgga attgttttga cgtgtgatat aattgatggg aatattaggc aacgaggtcg
      781 aaagatttat ccaggttaca ccttgatcgt tggtttgaaa aactttattg ccacccgaaa
      841 atcccgaata ggtaatccag gcacgattgg ggtcggtatt actgcatgca atggatgaaa
      901 tagtgccggc aggtacattg cttatggtgg tccagttggt gccaccatta ttggttttgt
      961 aaagtgcacc tgctttggct gcccaaagca cctgattatt tgcagcactt acagcaattg
     1021 catttaaagt gcccgaatta ctaaacgaac ttattttagt ccatgtactg cctccgttat
     1081 tgctgcgcca aacatttaca aaacctgcat aaagtgtact agctgttata gggtcttgca
     1141 gccagggcgt aacccaggct ccggcttcgt taatgctacc gttggcgctg ctccacgtat
     1201 tgccaccatt ggtgctacgg tttaagctgc cattgtattg cgagccccac atgttttggt
     1261 cgttattcca gtcaataaaa gcaagcatgc catcaccacc cataacctct tgccaagtag
     1321 tgccattgca acggttggta ccattgtctt gccagccttg tataaataaa ctcgcattgg
     1381 tgctcgattg gccaaaacca tacatctggc taatttgtaa gccatcactt aaatcggtcc
     1441 aggtggttcc attatttacc gttttaaata atccgccatc gcaagctacc caaacagtat
     1501 tggcatcggt gtaaacaatt tcatgcacat cggcatgtac ataaggcgca cctgatccgg
     1561 tccaatgacc gtttaacgaa aacgatgaac caccatttgt actgcgccaa acatttacac
     1621 caccggttgt aacttcgttg gcattggtgg gcgaggcagc aaaacccaaa tcgtaccatc
     1681 cttggccgcc actatcgcca ccattcgagt cccaacctaa aatattgccc gggctcgata
     1741 cggttacctt ggtaaacgat g
//
LOCUS       DAOMAA010000476         1760 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_72405_length_1760_cov_2.913783, whole genome shotgun sequence.
ACCESSION   DAOMAA010000476 DAOMAA010000000
VERSION     DAOMAA010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72405_length_1760_cov_2.913783"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /gene="tsf"
                     /locus_tag="PLO59_06825"
     CDS             complement(<1..391)
                     /gene="tsf"
                     /locus_tag="PLO59_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013664734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HQV00857.1"
                     /translation="MSTTMTISAADVNKLRQQTGAGMMDCKKALTEANGDFEAAIDYL
                     RKKGAKVAANRSDRDAKEGYIIAKTNDDATQGYLIALNCETDFVAKNADFTKFANDVL
                     DAAIKNNATTVDAIKDMAFEGVTVADKL"
     gene            complement(430..1239)
                     /gene="rpsB"
                     /locus_tag="PLO59_06830"
     CDS             complement(430..1239)
                     /gene="rpsB"
                     /locus_tag="PLO59_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013634190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HQV00858.1"
                     /translation="MGIVTQEALLEAGVHFGHLKRKWNPKMAPYIFMERNGIHIIDLN
                     KTEAKLEEAAAAVRQIAKSGKKILFVATKKQAKDIVANAAKSVNMPYITERWPGGMLT
                     NFATQRKSVKKLTGFDKMMTDGTFDVISKKEKLQISRQKAKMEKFLGSITDLTRLPAA
                     IFIVDILKEHIAVAEARKLNIPTIGICDTNTDPNLIDFPIPANDDATKSIALMMEVMV
                     KAIEEGLAERKVDKESDREKEVAEEDAQGEKDAVIAGDDEGKKGLRSRKKK"
     gene            complement(1285..1671)
                     /gene="rpsI"
                     /locus_tag="PLO59_06835"
     CDS             complement(1285..1671)
                     /gene="rpsI"
                     /locus_tag="PLO59_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016200426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HQV00859.1"
                     /translation="MEINHTIGRRKTSIARIYLQPGTGVITVNNKDYKTYFPSMPLQY
                     AVTQAFELSNTKEQYDVTVNVIGGGVTGQAHAIRLAISRALCDINPEHRSALKPHGLL
                     TRDPRMVERKKPGQKKARKRFQFSKR"
BASE COUNT          433 a          349 c          324 g          654 t
ORIGIN      
        1 atagcttatc ggcaactgtt acgccttcga aagccatatc ttttattgca tcaactgtgg
       61 tagcattgtt tttaatggca gcgtctaaaa catcgtttgc aaattttgta aaatcagcat
      121 tttttgccac aaaatcagtt tcgcaattta aagctattaa atagccttgt gttgcatcgt
      181 cattggtttt agcaatgata tatccttctt tggcatcgcg gtcgctgcgg ttggctgcta
      241 cttttgcgcc ttttttacgt aagtaatcaa tggctgcttc aaaatcaccg ttagcttcgg
      301 taagggcttt cttacaatcc atcattccgg caccggtttg ttggcgtagt ttatttacat
      361 cggcagcgct tatagtcata gttgttgaca tgaatttatt ttaatttata tttctctgat
      421 aaggctttat tattttttct tacgtgagcg taatcctttt tttccttcat catcacctgc
      481 aattacagca tccttttctc cttgtgcatc ttcttcggcc acttcttttt cgcgatctga
      541 ttctttatca actttgcgtt cggccaaacc ttcttcaatt gcctttacca ttacttccat
      601 cattaatgct attgacttgg ttgcatcatc gttggcaggt attgggaagt caatcagatt
      661 cggatctgtg tttgtatcac atataccaat agttggtata tttaatttac gtgcttcggc
      721 aacagcaata tgttctttta gtatatcaac tataaatata gcggctggca aacgtgttag
      781 gtcggttatg ctacctaaaa atttttccat tttagccttt tggcggctta tctgaagttt
      841 ttcttttttc gaaattacat caaaggtacc atcagtcatc atcttatcaa acccggttaa
      901 tttcttaacc gacttacgtt gtgtagcaaa gtttgttaac ataccgcctg gccaacgctc
      961 ggttatataa ggcatgttaa ctgattttgc tgcattggca acaatgtcct tagcttgttt
     1021 tttagtagct acaaacaata ttttttttcc gcttttagct atttgacgca cagccgctgc
     1081 tgcttcctct aacttagctt cggttttgtt taaatcaatg atgtgaattc cattgcgctc
     1141 cataaaaata tagggagcca tttttgggtt ccatttacgc tttaagtggc caaaatgcac
     1201 acctgcttct aacaatgctt cttgagttac tattcccatt tatgttgaat gttgtgaata
     1261 ttaaaaattg agtagtggta aattttaacg ttttgagaat tggaaacgct tgcgggcttt
     1321 tttctgaccc ggttttttac gttccaccat ccttgggtcg cgggtaagca atccatgcgg
     1381 ttttaaagcc gaacgatgct cggggttgat gtcgcataaa gccctcgaaa ttgctaatct
     1441 gattgcatgt gcctgaccgg ttactccacc accaataaca tttacggtta catcatactg
     1501 ctccttagtg tttgaaagtt cgaatgcttg tgttacagca tattgtaatg gcattgacgg
     1561 aaagtacgtt ttataatctt tattattaac ggtaattaca cctgtgcccg gctgtaaata
     1621 tatacgcgct attgaagttt ttctgcggcc aattgtatga ttgatttcca ttagggatac
     1681 ttaaattata tagtaaatgt tgatggtttt tgtgcttcat gcggatgcga atctccggca
     1741 tataaaaaca aattagattt
//
LOCUS       DAOMAA010000477         1744 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_73701_length_1744_cov_2.512137, whole genome shotgun sequence.
ACCESSION   DAOMAA010000477 DAOMAA010000000
VERSION     DAOMAA010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73701_length_1744_cov_2.512137"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            193..1476
                     /locus_tag="PLO59_06840"
     CDS             193..1476
                     /locus_tag="PLO59_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HQV00860.1"
                     /translation="MKTMEQKGLAVSKMAEGLIGSEIIKIAGEVKNKMANGEKIYNFT
                     IGDFDPKIFPIPAELTQEIKDAYTLGETNYPEANGILGLRKQVSKFISDREKINYDAD
                     EVVIACGARPIIYAIFRTLVDSNDTVIFPIPSWNNNHYCHMTDANVVEITTQAENNFM
                     PTADELRPHLKGATLIALCSPLNPTGTVFKKEVLEEICDMILAENASRSANEKPLYLM
                     YDQIYWVLTHGQTQHYNPVSLRPAMRNYTVFVDGISKCFAATGLRVGWSIGPKFITDR
                     MRAILSHVGAWAPRAEQIATEKYLSDSQKVDTFLNVFKTELSLRLEALYNGINALQQK
                     GYKVAAIAPQAAIYLTVQFNLTGYTTKDGMLLTNARDVTKFLLNDAKLAVVPFYAFGS
                     DDSNQWYRISVGTCKKEEIPQVLQLLEAALESLSK"
     gene            complement(1558..>1744)
                     /locus_tag="PLO59_06845"
     CDS             complement(1558..>1744)
                     /locus_tag="PLO59_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00861.1"
                     /translation="FAGAEKIKPWADVNLSASYRYSKVLSFYVNLNNVAFVRYQQWYN
                     YPAYRFNAMAGITYAFR"
BASE COUNT          598 a          313 c          319 g          514 t
ORIGIN      
        1 cgcgcgaaga aaatttattt gttgatactc ttttctctaa atgggaacct gatttaatca
       61 aataaatacc tccgctttta ttggtgttta aatcgctcgt atgaacttag tttaagcttc
      121 aataaatcgc caagagctta ttatcaattt taaaaacaag tcacaaattt tgaaaatatt
      181 atttataata aaatgaaaac aatggaacaa aaaggattag cagtttcgaa aatggctgaa
      241 ggattaattg ggtcggaaat tattaagata gcaggtgagg ttaaaaacaa aatggctaat
      301 ggcgaaaaaa tttacaattt tacgattggc gatttcgatc caaaaatttt tccaattccg
      361 gccgagctaa ctcaagaaat aaaagatgca tatacacttg gtgaaactaa ttaccctgaa
      421 gcaaacggta ttttgggatt acgtaaacag gtaagtaaat ttatttcaga cagagaaaaa
      481 ataaactacg atgccgatga agtagtgata gcctgtggtg ccaggcccat catttatgcc
      541 attttcagaa ctttagttga tagtaacgat accgttattt ttccaattcc atcgtggaat
      601 aacaaccatt actgccacat gaccgatgcc aacgtagtgg aaattaccac tcaagctgaa
      661 aataatttta tgcctactgc tgatgagcta cgcccgcatt taaaaggggc tacattaata
      721 gcattatgct cgccacttaa tcctacaggt accgttttta aaaaggaagt actcgaagaa
      781 atttgtgata tgatacttgc cgaaaacgca agccgaagcg ccaatgaaaa accattatat
      841 ctgatgtatg atcaaattta ctgggtactc acacatggtc aaacgcagca ttataatccg
      901 gtatcgttac gccctgccat gcgcaactac accgtatttg ttgatggtat ttcaaaatgt
      961 tttgctgcaa ccggattacg tgttggttgg agtattggac ccaaatttat taccgacagg
     1021 atgcgcgcaa tactatcaca tgttggtgcc tgggcaccac gtgccgaaca aattgcaacc
     1081 gaaaaatatt taagtgacag ccaaaaagtt gatacctttt taaatgtatt taaaaccgag
     1141 ttgtcgttgc gattagaagc gctttacaat ggcataaatg ctttacagca aaaaggttat
     1201 aaagtagctg ctattgcacc acaagctgcc atttacctta cggtacaatt taatttaaca
     1261 ggctatacga caaaagacgg tatgttgtta accaatgcac gtgatgtaac aaagttttta
     1321 cttaatgatg ctaaattagc agtagttcct ttttacgctt ttggttctga tgatagtaat
     1381 caatggtatc gaatttcagt aggtacttgc aaaaaagaag aaatacccca agtgctacaa
     1441 ttgttagaag cagcacttga atcgttatcg aaataaattg cttacaacaa ttaaataaaa
     1501 tgggcactac ttacggtgcc cattttattt taaccaaatt caatctgata tacttaatta
     1561 tctaaaagca taggttatgc cggccatggc attaaaacgg tatgccggat aattgtacca
     1621 ctgctgatag cgaacaaatg ctacattgtt aagattaacg tagaatgata atacttttga
     1681 ataacgataa gatgcactta ggttaacatc ggcccacggt ttaatttttt cagcacctgc
     1741 aaaa
//
LOCUS       DAOMAA010000478         1741 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_73950_length_1741_cov_2.256228, whole genome shotgun sequence.
ACCESSION   DAOMAA010000478 DAOMAA010000000
VERSION     DAOMAA010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73950_length_1741_cov_2.256228"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1091)
                     /locus_tag="PLO59_06850"
     CDS             complement(<1..1091)
                     /locus_tag="PLO59_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00862.1"
                     /translation="MHIVLIYTKHITNRLTYIANVLLPESHFQIKFTTSENEFNTSTG
                     TKINYSNTQFDNAIQIVPHGLLAQKHIENIAVDVLSKNGLIQLFNNKSELGFDVLAAS
                     FYLISRYEEYLPYQPDAHGRFTSQQSIAGKHGFLSQALVNRYMLLLHQKLHEICKLPL
                     PPIRYNALITMDIDSAYAFLGKGYWRTIGAYAKLLLTCNFAKVAQRTQTLLGKADPFN
                     TYSFFASCLRPYNLKPMFFILMGDYGRYDKNISYQGKYMQKLIAQLKTYGTIGLHPSY
                     YAVAKNKIGTECKRIQKITHEDVQHSRFHFLRLKLPQAYRQLLDAGITNDYSMGYADC
                     AGFRASTSLTHYFFDIENDVTTPLQIHPLT"
     gene            complement(1116..1736)
                     /locus_tag="PLO59_06855"
     CDS             complement(1116..1736)
                     /locus_tag="PLO59_06855"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007096922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQV00863.1"
                     /translation="MEQYAAMHSDAESDLLKSLNRHTYARVLQPRMLSGHLQGRILAM
                     LSRMIQPKAILEIGTYTGYSALCLAEGLQTDGVLHSIDINPELHETALQFVQQSPYHN
                     QIHLHVGDALHIIPAFEIQFDLVFIDADKENYCTYLDLAINCVHSGGYIIADNVLWSG
                     NVVKPENEQDRNTAPVIAFNKKVLADVRLRKVLLPVRDGLMICQKI"
BASE COUNT          516 a          318 c          329 g          578 t
ORIGIN      
        1 gttagcgggt gaatttgcaa tggcgtagta acatcatttt cgatgtcaaa aaaataatgt
       61 gttaatgatg tgcttgctct gaagccggca caatcggcat aacccatgct gtaatcgttt
      121 gtaatgcctg catccagtaa ttggcggtaa gcttgtggca atttaagtct taaaaaatga
      181 aaccggctat gttgcacatc ttcatgcgtt attttttgta ttcgtttgca ttcagtgcca
      241 attttattct tagctactgc atagtatgat gggtgcaagc caatggtacc ataggttttt
      301 agttgcgcaa ttaatttttg catgtatttg ccttggtaac tgatattttt gtcataccgc
      361 ccataatcgc ccatcaaaat aaaaaacatt ggctttaggt tatatggccg taagcaagat
      421 gcaaaaaatg agtacgtatt aaatggatcg gctttaccta acagtgtttg tgttctttgc
      481 gcaactttag caaaattgca ggttaggagc aactttgcat aggcacctat tgtgcgccaa
      541 tagcctttac ctaaaaatgc ataagcgcta tcaatatcca tagttattaa tgcattgtaa
      601 cgtattgggg gtaaaggcag tttacaaatt tcatgtagct tttggtgcag caataacatg
      661 tatctgttta caagtgcctg gctcaaaaaa ccatgtttac ctgcaatact ttgctgcgat
      721 gtaaaccggc catgtgcatc gggttggtac ggtaaatatt cttcataacg acttatcaaa
      781 taaaatgatg cagccaatac atcaaaacca agttccgatt tattattaaa aagttgtatt
      841 aacccatttt tacttaaaac atcaacagca atattttcga tatgtttttg tgccaataat
      901 ccatgtggca caatttgaat tgcattatca aattgagtgt tactgtaatt tatttttgtg
      961 ccagttgacg tgttaaattc gttttcagat gttgtaaatt taatttgaaa atgggattcc
     1021 ggcaaaagca catttgctat gtaggttaac ctgttggtta tgtgtttggt ataaattaat
     1081 actatatgca tgttgtgact ttacacgtag caaggttaaa ttttttgaca aatcataagc
     1141 ccatcgcgca cgggtagtaa cactttgcgt aaacgtacat cggccaacac ttttttatta
     1201 aacgcaatta caggagctgt gtttctatcc tgttcgtttt caggtttaac cacattacca
     1261 ctccataaaa cattatcggc aattatataa cctccactgt gtacgcagtt tattgccaga
     1321 tctaaatacg tacaataatt ttctttatcg gcatcaataa aaaccaggtc aaactgtatt
     1381 tcaaatgcag gtatgatatg taatgcatcg cctacatgca aatgaatttg attatggtaa
     1441 ggcgattgtt gcacaaactg caacgcagtt tcatgcaact cggggttaat atcaattgaa
     1501 tgtaatacac catctgtttg tagtccttcg gccaaacata aagcggagta accggtatag
     1561 gtaccaattt ctaatattgc tttgggttgt atcatgcggc ttaacatagc caatatgcga
     1621 ccttgcaaat gacccgacaa catacgtggt tgcaacaccc tggcataggt atgccggttt
     1681 aatgatttta ataaatcact ttcggcatcg ctatgcatgg ccgcatattg ttcaatgttt
     1741 t
//
LOCUS       DAOMAA010000479         1734 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_74464_length_1734_cov_3.269208, whole genome shotgun sequence.
ACCESSION   DAOMAA010000479 DAOMAA010000000
VERSION     DAOMAA010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74464_length_1734_cov_3.269208"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1369
                     /gene="guaB"
                     /locus_tag="PLO59_06860"
     CDS             <1..1369
                     /gene="guaB"
                     /locus_tag="PLO59_06860"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016778364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HQV00864.1"
                     /translation="SSLFTRNIKLNAPIVSAAMDTVTESALAITIAQEGGIGVLHKNM
                     SIEAQAAEVRKVKRSESGMIQDPITLHQDATIGQALDIMRENKIGGIPIVNHQGILVG
                     ILTNRDLRFERNMHRKVSEVMTKENLVTVTGVTNLQKAEVILMEYKIEKLPVVDKKQK
                     LIGLITYKDIIKFKSRPDSCKDSFGRLRVAAAVGITADMLQRVEALVKVGVDAITIDT
                     AHGHSKGVIDALKRTKKSYPALDVVVGNVGTGAAALALAKAGADAVKVGIGPGAICTT
                     RIVAGVGVPQLFAVYDVAQSLKNTGVPVIADGGIKYSGDVAKAIAAGAHSVMMGSMFA
                     GVEESPGETIIFEGRKFKTYRGMGSIEAMREGSKDRYFQDVEDDIKKLVPEGIVGRVP
                     YKGTLAEEMYQLKGGLRASMGYTGSANIKALQNAQFIRITQAGVAESHPHSIQITKEA
                     PNYKR"
     gene            complement(1399..>1734)
                     /locus_tag="PLO59_06865"
     CDS             complement(1399..>1734)
                     /locus_tag="PLO59_06865"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQV00865.1"
                     /translation="HITHDLLKHALTDITVTTFTNGKELIDHLRIHTDLSNTCILTDL
                     DMPVMNGFEATHIIRNELKLTIPIIALTASVFIGDEAEFKQAGFNGMVSKPFVTIELM
                     KAIAKTMAQ"
BASE COUNT          520 a          315 c          415 g          484 t
ORIGIN      
        1 aagcagcctg tttacacgca atataaaatt aaatgcccct attgttagcg ctgcaatgga
       61 taccgtaacc gaaagtgcat tagctattac cattgcacaa gaaggcggta taggtgtgtt
      121 gcataaaaac atgagtattg aagcccaggc tgccgaagtg cgcaaagtaa aacgcagcga
      181 aagtggcatg atacaagacc cgattacctt gcatcaggat gcaaccattg gtcaggcatt
      241 agatattatg cgcgaaaata aaattggtgg cattcccatt gtaaatcatc aaggtatttt
      301 ggtaggcata cttaccaacc gcgatttgcg tttcgagcgc aacatgcacc gcaaggttag
      361 cgaagtaatg accaaagaaa atttagttac ggttacaggt gttacaaatt tgcaaaaggc
      421 cgaagtaatt ttaatggagt ataaaatcga aaaattgcct gtggttgata aaaaacaaaa
      481 gctaattggt ttgataactt ataaggatat tataaagttt aaatcgcgtc ccgactcatg
      541 taaggattcg tttggccgtt tgcgagttgc tgcagctgtt ggcattacag ccgatatgct
      601 gcaacgtgtt gaagccttgg taaaagtagg cgttgatgca attactattg atacagcaca
      661 cggccacagt aagggcgtaa ttgatgcatt aaaacgtacc aagaaatcct atccggcatt
      721 agatgttgtt gtaggtaatg tgggtaccgg agctgcggca ttggcattgg ccaaagctgg
      781 tgccgatgct gttaaagtag gcattgggcc cggtgctatt tgcaccacac ggatagttgc
      841 aggtgtaggt gtaccccagt tatttgctgt atatgatgtt gctcaatctt taaaaaatac
      901 gggtgtacct gtaattgccg atgggggtat taaatacagt ggcgatgtgg ctaaagccat
      961 agctgccggt gcgcatagtg taatgatggg tagtatgttt gcaggagttg aagaaagtcc
     1021 gggcgaaacc atcatttttg aaggccgtaa atttaaaacc tatcgtggta tgggcagcat
     1081 tgaagccatg cgcgaaggta gtaaggaccg ttattttcag gatgtggaag atgacattaa
     1141 aaaactggta cccgaaggta tagtaggtcg tgtgccatat aaaggtacac tggccgaaga
     1201 aatgtatcaa cttaaaggcg gtttacgtgc cagcatgggt tatacaggct ctgcaaatat
     1261 taaggctttg caaaacgcgc aatttatccg aataacacaa gccggtgttg ccgaaagcca
     1321 tccacactcc attcaaataa ctaaagaagc acccaattat aaacgataga ccggcagtgc
     1381 tgcttgtttt aaacttttct attgcgccat tgtttttgca atagctttca tcagttcgat
     1441 ggtaacaaaa ggtttactaa ccataccatt aaaaccagct tgtttaaact ccgcttcatc
     1501 accaataaat acactggctg taagcgcaat tatgggtatg gtaagtttta attcgttgcg
     1561 tataatgtga gttgcttcaa agccattcat aacgggcata tccaggtcag ttagtatgca
     1621 ggtgttggat aaatcggtat ggatacgcag gtgatctatc agttcttttc cgtttgtaaa
     1681 tgttgtaact gttatatctg taagtgcatg cttaagcaag tcatgtgtta tgtg
//
LOCUS       DAOMAA010000480         1732 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_74636_length_1732_cov_2.715564, whole genome shotgun sequence.
ACCESSION   DAOMAA010000480 DAOMAA010000000
VERSION     DAOMAA010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74636_length_1732_cov_2.715564"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..174)
                     /locus_tag="PLO59_06870"
     CDS             complement(<1..174)
                     /locus_tag="PLO59_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010135514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HQV00866.1"
                     /translation="MLDIREINEKIERESAFVDLLNLEIGKKIVGQKYMVERLMIGLL
                     SNGHILLEGVPGLA"
     gene            485..>1732
                     /gene="mtaB"
                     /locus_tag="PLO59_06875"
     CDS             485..>1732
                     /gene="mtaB"
                     /locus_tag="PLO59_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009642077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HQV00867.1"
                     /translation="MLNTVAFHTLGCKLNFTETTVIGKQFKAAGFNKVAFDQPSDVYV
                     INTCSVTDNADKECRKLVSKALQLHANAFIIIVGCYAQLKPHEIAAIPGVDLVLGANE
                     KFNVVSYLHTLNKQQQTQVHACEINDVNTFVYAQSETERTRIFLKVQDGCDYTCTFCT
                     IPLARGKSRSDTITNALKRITEAVNNGGREIILTGVNLGDFGYTTDNNEKRSTTFFDL
                     IKAIDALPLEVRIRISSIEPNLLTKEIIDFVAQSKKFVPHFHIPLQSGSDEILKKMRR
                     RYLSKLYADRVAYIKNKMPHACIGVDVIVGFPSETDQHFNETYQFIQQLDVSYLHVFT
                     YSERLNTPAAQMGAVVPVHIRKQRNQMLRILSEKKLRHFYSQQLSNTYEVLFEADNKG
                     GYMHGFTPNYIKVKTPYNVHLVNQ"
BASE COUNT          564 a          310 c          308 g          550 t
ORIGIN      
        1 tgccaggccg ggcacacctt ctaacaaaat gtgtccatta cttaacaagc ctatcatcaa
       61 tcgctcgacc atatattttt ggccaacaat ctttttgccg atttctaaat ttaataaatc
      121 tacaaaggcg ctttcgcgtt caattttttc attaatctct ctgatatcta acataatttt
      181 agaaatggat ttaacgtgtt ttaattaata aacggttttt aagttaacca aatatgagtt
      241 tatgtaatgg cggcaatttt agtacaatca ccatacatta agtttgaata cttagttaag
      301 cgctgttaaa gaaatgttaa ccgaattgct tcgaaacact tgtaaaataa ggcatcgggc
      361 ataatgttgt ctttttttac aagtgtttta aaatggatta ttgtgtcaaa tactttacaa
      421 cactattgcc atatcagata gccaatttct tatttgggtt ttatgattta ctttcgccac
      481 ccttatgcta aatactgttg catttcatac tttaggttgt aaactcaatt ttaccgaaac
      541 aacggtaatt ggtaaacaat ttaaagcagc cggctttaat aaagttgcgt ttgaccagcc
      601 aagcgatgta tatgtaatta atacctgttc ggttactgat aatgccgata aagagtgccg
      661 caagcttgtt agcaaagcat tgcaactgca tgcaaatgca tttataatta ttgtaggttg
      721 ctatgcccag cttaagccgc atgaaatagc tgccataccc ggtgttgatt tggtgttggg
      781 tgcaaatgaa aaatttaatg tagttagcta tttgcatact ttaaacaagc aacaacaaac
      841 tcaagtacat gcttgcgaaa taaatgatgt aaataccttc gtttatgcgc aaagcgaaac
      901 agagcgcaca cgtatatttt taaaggtgca agatggctgc gattacactt gtaccttttg
      961 caccattcct ttggcgcgtg gtaaaagcag aagcgataca attaccaatg ccttaaagcg
     1021 cattacagaa gcagttaata atggcggacg cgaaataatt ttaaccggtg ttaatttagg
     1081 cgactttggt tatactactg ataataacga aaagcgaagc accacatttt ttgatttgat
     1141 aaaagcaatt gatgcattac cattagaagt gcgcatacgc atttctagta ttgaacccaa
     1201 tttgcttacc aaagagatta ttgattttgt tgcacaatca aaaaaatttg tgccgcattt
     1261 tcacattcca ttgcaaagcg gcagtgatga aattttgaaa aaaatgcgca ggcgttatct
     1321 gtctaaactt tatgccgatc gtgttgcata tataaaaaac aaaatgccac atgcttgtat
     1381 tggtgttgat gtaattgttg gatttccgtc tgaaacggat caacatttta atgaaaccta
     1441 tcagtttatt cagcaattag atgtttcata cctgcatgtg tttacataca gcgaaagact
     1501 taatacaccg gctgcccaaa tgggagcagt tgtaccggtt catatccgca aacaacgcaa
     1561 tcagatgtta cgtatattaa gcgaaaagaa gctaaggcac ttttacagtc aacagctttc
     1621 aaatacgtat gaagtattgt ttgaagcaga taataaaggt ggctatatgc atggttttac
     1681 acccaattac atcaaagtta aaacacctta caacgtacat ttggtaaatc aa
//
LOCUS       DAOMAA010000481         1732 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_74648_length_1732_cov_2.435301, whole genome shotgun sequence.
ACCESSION   DAOMAA010000481 DAOMAA010000000
VERSION     DAOMAA010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74648_length_1732_cov_2.435301"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..824
                     /locus_tag="PLO59_06880"
     CDS             <1..824
                     /locus_tag="PLO59_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017258630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HQV00868.1"
                     /translation="TGGTICGTAKYLKEKNPNIKIFGIDTYGSVFKKYKETGIFDKNE
                     IYPYVTEGIGEDFLPENVNFDLIDLFEKVTDKDAALMTREIAKQEGIFAGNSAGSAMA
                     GLMQLANQFKEGDVVVVIFHDHGTRYLGKMFNDDWMREKGYFDKKGLLAKDLVTSQKG
                     EVASLESTATIEDALKLMEKMSYSQIPVTTAKRMVGSLNETDIYRAIVANPEVKSKAI
                     DTIMKPAFPFVDISTPIDALSQMLSAEHDAVLVRDFKIDKTYIITRSDVLNALTR"
     gene            836..1642
                     /locus_tag="PLO59_06885"
     CDS             836..1642
                     /locus_tag="PLO59_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00869.1"
                     /translation="MRTALIILLSVFWSYLNAQQNENRFRRFHFNTQVGFNSVSIDEV
                     ISKQFAKKLLQGSLVYFNDNLIIGGAPDNRSGKAPVLNLGFEYSLYPRFLIGVGMHGI
                     PATNIVGYFINDTVINGSNQTIVYDRVEELVKGITIKAYIMYALKPYKANTGLGWQLA
                     LGGGLSTNLLSVKQSYFSNSYDSLNQTLEETPSIYNQKASSVGAFLVGRAEIHFTRSF
                     SVLGDFNCLFDTGVEIDETQFNGNQAIQQIDAHQVSLQSYLVTVGIGLHF"
BASE COUNT          570 a          293 c          363 g          506 t
ORIGIN      
        1 gtactggtgg caccatttgc ggcacagcaa agtatttgaa agagaaaaat ccgaacataa
       61 aaatatttgg tattgatact tatgggtcgg tgtttaagaa atataaagaa accggcatat
      121 ttgataagaa tgaaatttac ccatacgtaa ccgaaggtat tggcgaagat tttttacctg
      181 aaaatgtaaa cttcgacctg atagatttat tcgaaaaagt tacagataag gatgcagccc
      241 taatgacacg cgaaattgca aagcaagaag gaatttttgc aggcaacagt gccggcagtg
      301 caatggccgg tttaatgcaa ttagccaatc agtttaaaga aggcgatgtt gttgtagtaa
      361 tttttcatga ccatggaaca cgttatttgg gcaaaatgtt taatgacgat tggatgcgcg
      421 aaaaaggata ttttgataaa aagggattgt tagccaaaga cttagttaca tcgcaaaaag
      481 gcgaagtagc ttcattagaa agtacagcaa ccattgaaga tgcattaaag ctgatggaaa
      541 aaatgagcta ttcgcaaata ccggttacaa ctgcaaaacg catggttggt tcgctaaacg
      601 aaacagatat ttaccgcgcc atagttgcca atcccgaagt aaaatcgaaa gctatagata
      661 cgattatgaa accggcattt ccgtttgtgg atataagcac acctatagat gcattgtcgc
      721 aaatgttatc ggccgagcat gatgcggttt tggtacgcga ttttaaaatt gataaaacct
      781 atatcattac ccgcagtgat gtattaaatg cactaacacg ataagggtaa taaatatgcg
      841 cacggcactg attatcctgc taagtgtatt ttggagttac ttaaatgcac aacaaaatga
      901 aaatcgtttc aggcggtttc attttaatac acaagtaggc tttaactcag tgagcattga
      961 cgaagtaatt tcgaaacaat ttgccaaaaa acttttacaa ggtagtttgg tctattttaa
     1021 cgacaaccta attattggcg gtgcacccga taaccgatca ggcaaagcac cggtattaaa
     1081 tttgggtttt gaatatagtt tgtatccccg ttttttaata ggtgtgggca tgcatggcat
     1141 acctgcaact aatattgtag gctactttat aaatgatacg gttatcaatg gctcaaacca
     1201 aaccattgtt tacgacaggg tagaagaatt agttaaggga attaccataa aagcatacat
     1261 catgtatgcc cttaaaccct ataaagccaa tactggactt ggttggcagt tagccctggg
     1321 tggcggctta tcaaccaatt tgttaagtgt taaacaatcg tattttagta acagctatga
     1381 ttcgctcaat caaacccttg aagaaacgcc ctccatttat aatcagaaag caagcagtgt
     1441 gggagcgttt ttagtaggcc gtgccgaaat tcactttacc cgaagttttt ctgtactggg
     1501 cgattttaat tgcctgtttg ataccggtgt tgaaattgac gaaacacaat ttaatggtaa
     1561 tcaggccata caacaaattg atgcacatca ggtatcgctg caatcgtatt tagtaacagt
     1621 gggcataggt ttgcattttt aattgttaca attaaacttt ttgagttaat agatatggct
     1681 gtttatccta aaaggtttat tgatcagttg caacgcgagg tctgtttaaa tt
//
LOCUS       DAOMAA010000482         1731 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_74688_length_1731_cov_3.195107, whole genome shotgun sequence.
ACCESSION   DAOMAA010000482 DAOMAA010000000
VERSION     DAOMAA010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74688_length_1731_cov_3.195107"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1731)
                     /locus_tag="PLO59_06890"
     CDS             complement(<1..>1731)
                     /locus_tag="PLO59_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018619012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HQV00870.1"
                     /translation="VANRGEIAIRALRAAAELGIRTIAVYTYEDRYSLHRYKADEAYQ
                     IGPDSEALKPYLDIEEIIRIAKKYEADAIHPGYGFLSENVNFARRCREEKIIFVGPKP
                     EVMEQLGDKVASKKVARACGVPLIEDNKEKLTSAKVALAEAKRIGFPVILKASAGGGG
                     RGMRVINAEADLEKSFNEAKREAGNAFGDDTIFIEKFVTNPKHIEVQILGDEYGNIVH
                     LFERDCSVQRRFQKVVEIAPAPSLKQETKDKLYEYALKLAQQVNYNNAGTVEFLVDEN
                     EQIFFIEVNPRIQVEHTVTEEVTGIDIVRSQILIADGKELASKEIDITSQESLKCNGF
                     AIQCRLTTEDPANGFKPDYGTIIAYRNAGGYGIRIDEGSSYQGVKVSPFFDSMLAKIT
                     AKGRTLEGASARMLRTLAEFRIRGVKTNMPFLENVLKHPVFKAGMCNVKFIETYPELF
                     EFKARMDSGTKVLSFLANTMVNGSADVQRVDETKKFTPGKVPAYNKFEPIKPGTKQLL
                     SQLGPDKFAQWLKDQKQIQYTDTTLRDAHQSLLATRVRTVDMLRVAEAYAYKHPQTFS
                     MEVWGGATFDV"
BASE COUNT          428 a          398 c          353 g          552 t
ORIGIN      
        1 acatcaaagg tggcaccacc ccaaacttcc atactaaatg tttgaggatg tttgtatgca
       61 taagcttcgg caacgcgtaa catatccacg gtgcgcacgc gtgtggctaa aagcgattga
      121 tgggcatcac gtaaagtggt atcggtgtat tgaatttgct tttggtcttt taaccattgc
      181 gcaaatttat cggggcctaa ctgcgacagt aattgtttgg taccgggttt tataggctca
      241 aacttattgt aagcaggtac tttaccgggc gtaaattttt ttgtttcatc aacacgttgc
      301 acatcggcac tgccattaac catggtgttt gccaaaaagc ttaacacctt tgtgccgcta
      361 tccatacgtg ctttaaactc gaaaagctcg gggtatgttt cgataaactt aacattgcac
      421 ataccggcct taaataccgg atgctttaat acgttttcga gaaaaggcat attggtttta
      481 acacctctta tgcgaaactc ggccagggta cgcagcatac gcgcactggc tccttctaat
      541 gtgcggcctt tggctgtaat ttttgccagc atgctatcaa aaaaaggcga aacttttaca
      601 ccctgataac tgctgccttc gtcaatgcgg ataccgtaac cacctgcatt tcggtatgca
      661 attatggttc cataatcggg cttaaagcca tttgccggat cttcggtagt aagcctgcat
      721 tgaatggcaa acccattaca ttttaacgat tcctgtgaag ttatatcaat ttcttttgat
      781 gcaagttctt taccatcagc tattaaaatt tgcgaacgta caatgtcaat gcctgttacc
      841 tcttcggtta ccgtgtgttc gacctgtatg cgtgggttta cttcaataaa aaatatctgt
      901 tcgttttcgt ccactaaaaa ctcaacggtg ccggcattgt tgtagttaac ttgctgtgcc
      961 agtttaagcg catactcgta taacttgtct ttggtttctt gtttgagtga tggtgccgga
     1021 gcaatttcta ctactttctg aaacctgcgt tgtacgctgc aatcgcgttc gaaaaggtgt
     1081 acaatattgc catactcatc gcctaaaatc tgtacttcga tatgcttggg attggttaca
     1141 aatttttcga taaatattgt atcgtcacca aaagcatttc cggcttcgcg tttggcttcg
     1201 ttaaacgatt tttctaaatc ggcttcggca ttaataaccc gcataccgcg gcctccgcca
     1261 ccggcacttg cttttaaaat caccggaaat ccaatgcgtt tggcttcggc taaagccacc
     1321 ttggccgaag taagtttttc tttattgtct tcaattaatg gtacaccaca ggcacgtgct
     1381 acttttttac tggccacctt atcgcccagt tgttccatta cttcgggctt ggggcctaca
     1441 aaaattattt tttcttcgcg gcacctacgt gcaaaattta cattctcact taaaaatccg
     1501 taacccgggt gtatggcatc ggcttcatac tttttagcta tgcggataat ttcttcaata
     1561 tctaaatacg gtttcagggc ttcgctatca ggcccaatct ggtatgcttc atcagctttg
     1621 tagcgatgca atgagtagcg gtcttcataa gtataaacag caatggttct tatacctaac
     1681 tcggctgcag cgcgcaatgc gcgtatggca atttcgcctc tgttggctac c
//
LOCUS       DAOMAA010000483         1730 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_74800_length_1730_cov_2.705672, whole genome shotgun sequence.
ACCESSION   DAOMAA010000483 DAOMAA010000000
VERSION     DAOMAA010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74800_length_1730_cov_2.705672"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="PLO59_06895"
     CDS             complement(<1..776)
                     /locus_tag="PLO59_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HQV00871.1"
                     /translation="MKTKQALLLLLICKATWAQETFPVNGITDKRHTTYAFTHATIYT
                     DYKTVLTDATLIIQDGIIKAVGNNVQIPAGAVISNTKGKYIYPSFIEPYGDYGVGLLK
                     TIAAEANNENQFLTNTKGAYAWNQAIRAEVNAVNQFASDKTQAESLLKAGFGTICSFN
                     KDGIVRGTACAVLLAPLKSQEVIIKQKAAAGLSFSKGSSSQDYPGSLMGCIALLRQTY
                     LDAQWYAGVKSQTNLSLGAFNEQQNLPQIFDCSNWQNQLRA"
     gene            complement(827..1501)
                     /locus_tag="PLO59_06900"
     CDS             complement(827..1501)
                     /locus_tag="PLO59_06900"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQV00872.1"
                     /translation="MVNNKPQLILIHGALGNALQMDVLAQNAKSVYDVYCFQCPYHDG
                     KPVELNKFTIPHFSNALLNFITENNLIKPKVFGYSMGGYLALYTQQIQPVFSKIVTYG
                     TKFNWSPEAALLETQKLQPNLIENKVPHFAGLLQAQYGTAWMQVVNATAALMHDISLN
                     KYLPDKILNPVVIPVTILVGDNDNMVTLSETQTATNWFANAQMHKVNGQHLLHKVDVT
                     TLLNFI"
BASE COUNT          513 a          296 c          335 g          586 t
ORIGIN      
        1 gcacgcaatt gattttgcca gttactgcaa tcgaatattt gcggcaaatt ttgttgttca
       61 ttaaatgcac ctaatgataa attggtttgg ctttttacac cggcatacca ttgcgcatct
      121 aaatatgttt gacgcaacaa tgcaatacaa cccattaacg aacccggata atcttgcgat
      181 gacgaacctt tgctgaaact taaaccggca gcggcttttt gtttaattat aacttcctga
      241 ctttttaacg gtgcaagcaa aactgcacat gcagtgccac gcacaatgcc atctttatta
      301 aaactacaaa tggttccgaa gccggctttt agcaatgatt cagcttgcgt tttatcgctt
      361 gcaaattgat ttactgcatt tacttcggcc ctgatggctt gattccaggc gtatgcaccc
      421 ttggtgtttg taagaaattg gttttcgttg ttggcttcgg ctgcaatggt ttttaatagt
      481 cccactccat aatcgccata aggttcgata aatgatggat aaatgtattt gccttttgta
      541 ttacttatta cagcacctgc cggtatttga acgttattgc ctacagcttt tattatgcca
      601 tcctgtatga taagtgtagc atcggtaagt acggttttat agtcggtata aatagttgca
      661 tgtgtaaaag cataagtagt gtggcgttta tcggtaatac catttaccgg aaaagtttct
      721 tgtgcccagg ttgctttgca aattagcagc agcagtaatg cttgtttagt tttcataaaa
      781 ataaaatatg tgccaaagta taaattagcg caaacgttgt tgtgtgctaa ataaagttta
      841 acagcgtagt tacatccact ttatgtaata gatgttggcc atttacttta tgcatttgtg
      901 catttgcaaa ccagttggta gcggtttggg tttcgctaag ggttaccatg ttatcattat
      961 cgccaactaa aatggtaacg ggtataacaa ccggatttaa aattttgtcg ggcaaatatt
     1021 tattcaaact aatatcatgc atcagtgctg ctgtagcatt tacaacctgc atccatgctg
     1081 tgccatattg cgcttgtaac aaaccggcaa agtgtggtac tttattttca attaagttgg
     1141 gttgtaattt ttgtgtttcc agtaatgcag cttcggggct ccagttaaat ttggtgccgt
     1201 aggttactat ttttgaaaaa acaggttgta tctgttgtgt gtataaagct aaatatccac
     1261 ccatgctata accaaatact ttgggcttga ttaggttgtt ttcggttata aaatttaaaa
     1321 gtgcattaga aaaatgtggt atggtaaatt tatttaattc aacaggcttg ccatcatgat
     1381 acggacattg aaaacaataa acatcataaa ctgattttgc attttgtgca agtacatcca
     1441 tttgtaaagc attgcctaat gcaccatgta taagtataag ctgcggttta ttatttacca
     1501 taattgaaat ttgtatttat ttgttttcgt tgttgaatta agctattaaa attcgaaaat
     1561 gcctacaccg gttgtaataa aagccatgct ggaaaagttt gataacaagg gcgaaaaatc
     1621 gggctggact tacatttata taaaggcaac tattgccaaa aaaattaaaa acacagcacg
     1681 ttcgtttaat gtaaaaggca gtttagatgc acatattttt aaaggcaaag
//
LOCUS       DAOMAA010000484         1729 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_74857_length_1729_cov_3.577061, whole genome shotgun sequence.
ACCESSION   DAOMAA010000484 DAOMAA010000000
VERSION     DAOMAA010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74857_length_1729_cov_3.577061"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /locus_tag="PLO59_06905"
     CDS             complement(<1..987)
                     /locus_tag="PLO59_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018622519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQV00873.1"
                     /translation="MKLLFTYLKNYKSLIAITLLLATINQCFSMLDPYILGKIIDTYV
                     KHYKELNLTQYLTGVAGLIGLAIGAAMISRIAKNFQDYFLSTITLQLGARIYQDGLKH
                     SLELPFQVFEDQRSGETLGILQKVRTDVEKLISAFVNILFTSVVGIVFVITVSLMTSW
                     FIGPLYILSIPILAFVSSVLSKKVKKVQKRIVGATTALAGSTTESLRNIELVKSLGLA
                     NQEIDRLNNTTTKILGLELEKVKYIRSISFIQGTIVNFLRNTILFLMMFLIFNNHLTE
                     GQFLMFFIYSFFIFGPLQELGNIILIYREAEVSLENLQRILNTPVEHVPVQPK"
     gene            1181..>1729
                     /locus_tag="PLO59_06910"
     CDS             1181..>1729
                     /locus_tag="PLO59_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004570024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HQV00874.1"
                     /translation="MSPALILITVAAYSALLFFIVWLTSRQSNNDSYFIGNKSSKWYI
                     VAYGMIGSSLSGVTFMSVPGAVYTTHFSYLQVVLGYLLGYAVIGWVLMPVYYKLNLTS
                     IYTYLEQRFGNVSYKTGSMFFIISRVIGAAFRMYIVVNVIQTFVLDQYGVPFWVTVTV
                     FMLLILLYTYEGGVKTIVWTDTL"
BASE COUNT          524 a          296 c          304 g          605 t
ORIGIN      
        1 cttgggctgc acaggtacat gttcaaccgg tgtgtttaaa atgcgctgca ggttttctaa
       61 cgatacttcg gcctcgcgat aaattaagat aatgttgccc aactcttgca atggcccgaa
      121 tatgaaaaat gagtaaataa aaaacatcag aaactgccct tcggttaagt ggttattaaa
      181 tatcaaaaac atcattaaaa ataagatggt atttcttaaa aaatttacaa tggtgccttg
      241 tatgaaacta atgcttctga tgtattttac tttttcgagt tcgagtccca aaattttagt
      301 ggtagtatta tttaaacgat caatttcctg attagccaaa cccaaacttt ttaccagttc
      361 aatatttcgt aacgattccg ttgtactgcc ggccaatgct gtggtagcac ctactatacg
      421 cttttgtacc ttctttactt ttttacttag cacactactt acaaaagcta aaatagggat
      481 tgataaaata tacaatggtc cgataaacca tgatgtcatt aacgaaactg taattacaaa
      541 tacaattcct accacacttg taaataatat gtttacaaag gccgatatca gcttttctac
      601 atcggttctt actttttgta atatacctaa tgtttcgcca ctgcgctgat cttcgaaaac
      661 ctgaaacggc aactctaacg aatgttttaa gccatcctga taaatgcgtg cacctaattg
      721 caaggttatt gtgcttaaaa agtaatcctg aaaatttttt gcaatgcgac ttatcatggc
      781 tgcacctatt gccaatccga ttaaaccggc cacgcctgtc aggtattgag ttaaatttaa
      841 ctccttataa tgctttacat acgtatcaat tattttaccc aatatgtacg gatcgagcat
      901 gctgaaacat tgattaatag tggctaaaag caatgttata gcaataaggc ttttataatt
      961 tttcaggtag gtaaaaagta gtttcatcta tgaatgtgta tcacgaaaat taagtagaag
     1021 gcaccggtta aaaatagttg cttaattaaa acgattgaag gcgatttaca ttttaaagtt
     1081 tattcgttat gtatgtataa ttgtttataa aatatcgtgc agttactatg gcttgttacg
     1141 tattaaaata gtttagcacg cttaaatacc tgattaaaat atgtcgcctg cacttatact
     1201 tattacagtt gccgcatact cggccttgct attttttatt gtatggttaa ccagcaggca
     1261 atctaacaac gattcttatt ttataggtaa caaatcgtct aaatggtata ttgttgctta
     1321 tggtatgatt ggctcatcgc ttagtggtgt tacttttatg agcgtacccg gtgcagttta
     1381 caccacacat tttagctact tacaggtggt gctgggttac ttattaggtt atgcagttat
     1441 tggttgggtg ttaatgcctg tgtattacaa attaaacctt acaagtatat atacctatct
     1501 cgaacaacgt tttggcaatg tatcgtataa aacaggctct atgtttttta taatatcgcg
     1561 ggtgataggc gctgcattta gaatgtacat tgtggttaat gtaattcaaa cttttgtttt
     1621 agatcaatat ggtgttccgt tttgggtaac tgttactgtt tttatgctgc ttatactttt
     1681 atacacttat gaaggcgggg taaaaaccat tgtttggact gatacttta
//
LOCUS       DAOMAA010000485         1729 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_74874_length_1729_cov_3.051374, whole genome shotgun sequence.
ACCESSION   DAOMAA010000485 DAOMAA010000000
VERSION     DAOMAA010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74874_length_1729_cov_3.051374"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>391)
                     /locus_tag="PLO59_06915"
     CDS             complement(<1..>391)
                     /locus_tag="PLO59_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HQV00875.1"
                     /translation="FVANPNMQPEFNGYDYLLERQLKPEAQKQVIEALHDAKIKPTAM
                     MDISDGLASELHHICKQSNVGCRIYEDKLPIGVKTHNLAEEFDFNPTTAALNGGEDYE
                     LLFTVPVTTFEVIKNIKDVHIIGHITDA"
     assembly_gap    392..401
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(402..963)
                     /gene="thiL"
                     /locus_tag="PLO59_06920"
                     /pseudo
     CDS             complement(402..963)
                     /gene="thiL"
                     /locus_tag="PLO59_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009422542.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
     gene            complement(1067..>1729)
                     /locus_tag="PLO59_06925"
     CDS             complement(1067..>1729)
                     /locus_tag="PLO59_06925"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00876.1"
                     /translation="MYEHDSVVAEGIIDENGRQQGNWILYDDKGNIKAEGAYKDGKRI
                     GAWRFFYPGKILQQEGNYEKGLPTGAWKWYYKDGKPLRDETYVKGKENGLSKEYDAYG
                     NIIAEGEYAGGLKTGPWNFNNNEYIVKGCQYIDGLKDGIWKSYNKDGSLYFEGKYIEG
                     NEEGDHTFYYGNGRIREVRSYKTGIPSGDWRFYDENGLLELTITYAGGQEQKYDGIRI
                     GQ"
BASE COUNT          452 a          375 c          314 g          578 t
ORIGIN      
        1 cggcatcggt aatatggccg ataatgtgca catcttttat atttttaatt acctcgaagg
       61 tggtaacggg tacggtaaac aacagctcat aatcttcgcc tccattaaga gcagcggtag
      121 ttggattaaa gtcaaactct tcggccaggt tgtgtgtttt aacaccaatg ggtagcttgt
      181 cttcataaat acggcaacct acattgcttt gcttgcatat atgatgtagt tcacttgcca
      241 atccatcgct aatatccatc atggccgtag gttttatttt tgcatcgtgc aatgcttcga
      301 taacttgttt ttgcgcttcg ggctttaatt gacgttccaa taaataatca taaccgttaa
      361 attcgggttg catgtttggg ttagctacaa annnnnnnnn ncgcgtttaa gtaattgcaa
      421 tcccatatat gcagcaccta aatcgcccgt tacgcaaatt aagtcggtgt cgtttgcacc
      481 gttgcgatac actaaatcat cggcattagc tataccaatg gctgtaacgc taataagcaa
      541 accactgttt gaagatgtgg tatcgccacc aattaaatca acaccataac ggttgcatgc
      601 cattgcaacg ccttcataaa actcctcaat ggcttctaac gaaaaccggt tgcttaatgc
      661 caggctgatg gttatttgtt tgggctgtgc attcatggca tacacatcgc taatgtttac
      721 taccactgat ttgtagccta aatgtttaag cggacaatat gttaagtcga aatgtatgcc
      781 ttctactaat aaatcggtag ttacaacggt gtggtggttg ccgctgttaa ttacggctgc
      841 atcatcacct atacctttaa tggtttcggg atttttaatt ttaacggttt gagcaatatg
      901 attaatcaaa ccaaattcgc ctaagctgtt taattcggta cgattgtttt tgttttcaaa
      961 catgaatgtt tttgttttaa atgaaaggtg taaaggtaaa ctaatggctt gttacgagtt
     1021 gttaggctta cgttagcggc atttacgttt tgtaaataca gcaaagttat tggccaatac
     1081 gtatgccatc gtatttctgc tcctgaccac cagcgtatgt tatggttagt tctaacaaac
     1141 cgttttcgtc ataaaagcgc caatcgccac tggggatacc cgttttataa ctacgaactt
     1201 cgcgaatgcg tccgttgcca taataaaaag tatggtcgcc ctcttcgtta ccttctatgt
     1261 atttgccttc gaagtacaag ctgccgtctt tgttatacga tttccatata ccatctttaa
     1321 gcccatcaat atattggcaa cctttaacaa tgtattcgtt gttattaaag ttccatggcc
     1381 cggtttttag gcctcctgca tattcgcctt cggcaataat gtttccataa gcatcatatt
     1441 ctttcgacaa gccattttct ttacctttta cataagtttc atcgcgcaat ggtttgccat
     1501 ctttataata ccatttccag gcaccggttg gtagtccttt ttcgtaattt ccttcttgtt
     1561 gtaaaatttt acccggataa aaaaaccgcc aggccccaat gcgtttgcca tctttatatg
     1621 caccctctgc ctttatattg cctttatcgt catacaaaat ccaattgcct tgttgccggc
     1681 cattttcgtc aataataccc tcggcaacaa cactatcatg ttcatacat
//
LOCUS       DAOMAA010000486         1726 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_75148_length_1726_cov_2.843806, whole genome shotgun sequence.
ACCESSION   DAOMAA010000486 DAOMAA010000000
VERSION     DAOMAA010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75148_length_1726_cov_2.843806"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /locus_tag="PLO59_06930"
     CDS             complement(<1..911)
                     /locus_tag="PLO59_06930"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HQV00877.1"
                     /translation="MPPDFNADTISRYVTYNKQLLSHLNIIVCLFYPKPYATMHILCY
                     AFLSMYCLLFFIQDVDAGNSDSLTFNTSTLTVKAGLSQGLVSAILQDKDGFMWFGTKD
                     GLNKYDGYGFTVYRHEKDNPNSLPDNYITGIAEDDNGNFWVGTNTKGLLLFDRKTEKF
                     YPVALPEQNFSNELKSPVEIQIHQGKLLVFNTVKVIYAISHVKPKNYQFIKLSKDKIC
                     WDSRLYLKQSGITPLLQLKTYLYQNKLFAWHDSTILNFELQPNGKYKLNKKHILSENG
                     LNNEVIYDISNYENENHQFMVRMREAKV"
     gene            1116..>1726
                     /locus_tag="PLO59_06935"
     CDS             1116..>1726
                     /locus_tag="PLO59_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00878.1"
                     /translation="MKQKLLATTVFGACMFLSMQQAKAQWSLTGNAATDTATNFIGTT
                     DNKGLKIKTNNVERMWFGNNGRIGIGTNTPVNTLDILNNTAVTMGIRSLVGGATMSMD
                     RGSATANAAFAYKSLGTVLWNSGMLGNDNFTIRNIVGGANCLVITPANNVAIGTGVPN
                     SKLSVFGNANISAELGIGTAPVTNNSLTIQSGDAAYGTMTLMKR"
BASE COUNT          544 a          314 c          329 g          539 t
ORIGIN      
        1 actttggctt cgcgcatgcg caccataaac tggtgatttt cgttttcgta attacttatg
       61 tcgtaaatta cctcattgtt taaaccattt tcagatagta tgtgtttttt attcagttta
      121 tattttccgt taggttgcaa ctcgaaattt aaaattgttg aatcatgcca tgcgaaaagt
      181 ttgttttgat acaaataagt tttcagttgc aaaagaggcg tgattcccga ctgcttaaga
      241 tacaatctgg agtcccagca aattttgtct ttgcttaatt taatgaattg ataattctta
      301 ggcttcacat ggcttatagc gtaaattact tttacagtat taaaaacaag caatttgccc
      361 tgatgaatct gaatttctac aggcgatttt aattcattcg aaaagttttg ttcaggtaag
      421 gctaccggat aaaatttttc ggtttttcta tcaaaaagta ataaaccttt cgtattggtt
      481 ccaacccaaa aattaccatt atcatcctcg gcaatgcctg ttatataatt atcgggtaac
      541 gaatttggat tgtctttttc atgccggtaa acagtaaaac catagccatc gtatttgttt
      601 aggccatcct tggtaccaaa ccacataaat ccatctttat cctgcaaaat agcagaaacc
      661 aacccttgcg ataaaccagc tttaacggtt agggtgctgg tgttaaaagt taagctatcg
      721 ctgttgccgg catctacatc ctgtataaaa aacagcaggc aatacatact taaaaaagca
      781 taacacagta tgtgcatggt tgcataaggt ttagggtaaa acaaacatac aataatatta
      841 agatgcgata gcagttgctt gttataagta acgtaccggc ttatcgtatc ggcattaaaa
      901 tcaggaggca ttttttatat atcaaaagtt gctgcccatt atttgttttc tatagttaac
      961 taataagctt ctcattcaga aagctatcat tgatattagt gagacgaatc gtttgtaact
     1021 acacatatca atagaatcaa ctatttacga aggtaaatgg ctgttgcact tttgtcattg
     1081 taaattattt attaaaccat taaaccaatt caacaatgaa acaaaaacta ttagcaacaa
     1141 cagtgtttgg tgcatgtatg tttttaagca tgcaacaagc aaaggcgcaa tggtcgctaa
     1201 cgggtaatgc tgctaccgac acagccacta actttattgg aaccacagac aataaggggt
     1261 tgaaaattaa aaccaacaat gtagagcgca tgtggtttgg caacaacggt cgtataggta
     1321 ttggtaccaa tacaccggta aacacactgg atattttaaa taacacagct gttactatgg
     1381 gcatacggtc attggttggt ggcgcaacca tgagtatgga tagaggaagt gcaactgcca
     1441 atgctgcatt tgcttacaaa agtttgggca cggtactttg gaatagcggt atgttgggca
     1501 acgataattt taccatacgt aatattgttg gtggtgccaa ttgccttgta attacacctg
     1561 caaacaatgt agctatcggt accggtgtgc ccaattcgaa actaagtgta tttggtaatg
     1621 caaacatatc ggccgaactg ggtattggta cagcacctgt taccaataac agccttacaa
     1681 ttcaatcggg cgatgcggct tatggcacaa tgacgctgat gaaacg
//
LOCUS       DAOMAA010000487         1723 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_75418_length_1723_cov_2.381295, whole genome shotgun sequence.
ACCESSION   DAOMAA010000487 DAOMAA010000000
VERSION     DAOMAA010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75418_length_1723_cov_2.381295"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..1204)
                     /locus_tag="PLO59_06940"
     CDS             complement(80..1204)
                     /locus_tag="PLO59_06940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQV00879.1"
                     /translation="MKSINIIVAIAALAIITACGNKSVDKKTELENLLKQQNELSAKI
                     DALRTELEKAGDSTVSGNIKNISTIVIKTDTFNHYVDVQGKVDANQSINANAQMQGNI
                     TAILVKEGDKVSAGQVLARIDDALLRQGLNEVKTQLDFATNIYNKQKALWEQQIGSEV
                     QYLTAKNNMESLARKHETMREQWAMTQIKSPISGTVDQVDIKLGQSIMPGMPCIHVVN
                     LSGLKIKAEVAESYIAKVNKGDKTRISIPDLGIDFESNVSYASTVVNPINRTFMVEVA
                     LPKADARLRPNMLSIMHVVDYTNANAVTVPVNVIQKGQNSNYVLIAETKDGKIIVAKK
                     DVIVGLTYSGVAEITSGLNNGDEVITVGYQDVNIGEAVKK"
     gene            complement(1243..>1723)
                     /locus_tag="PLO59_06945"
     CDS             complement(1243..>1723)
                     /locus_tag="PLO59_06945"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQV00880.1"
                     /translation="YYPTLAAYGNLGTSTNSEEFDLFESDKRWYWNAQVGARLTMPIF
                     TSGVRHNQIKQEELALRKIDNSTITLQEGFQLEYNTAKRNFINALASYDIQKKNRELA
                     NEIVRVSKIKYSQGVGSNLEVIDAESQQRAAESIFYTTVYDALIAKVELDKALGLIK"
BASE COUNT          441 a          355 c          307 g          620 t
ORIGIN      
        1 gcttgatggt ttaaattctt taaacttgtc tatcataaaa gtaagttgtt aggtgcttgc
       61 gtttaatgct ttttaccttt tattttttta cggcttcgcc aatgtttaca tcctgatatc
      121 caaccgttat tacctcatcg ccattattta aaccactggt aatttcggct actccgctgt
      181 aggttaatcc aacaataaca tcctttttag caacaataat ttttccgtct ttggtttcag
      241 ctatcaatac gtaattactg ttttgtcctt tctgtattac atttacagga acagttacag
      301 catttgcatt ggtgtaatca actacatgca taatgcttaa catgtttggc cgcaaacgtg
      361 catctgcttt aggtagtgcc acttctacca taaaagtacg gtttatagga tttacaacgg
      421 tgctggcata agaaacgtta ctctcaaagt caatacctaa atcgggaatt gagatgcgtg
      481 ttttgtcgcc cttattaact ttggcaatat aactttcggc tacttcggct ttaattttta
      541 agcctgataa gttaactaca tgtatgcaag gcatgccggg cataatgctt tgtccaagct
      601 taatatcaac ctgatcaacg gtgcctgaaa taggcgattt tatttgcgtc attgcccatt
      661 gttcgcgcat ggtttcgtgc ttgcgtgcca acgactccat attattttta gccgtaaggt
      721 attgtacttc gctgcctatt tgttgctccc aaagcgcctt ttgtttattg taaatattgg
      781 tagcaaaatc taattgtgtt ttaacttcgt taaggccttg gcgtagcaga gcatcgtcaa
      841 tacgggccaa cacctgaccg gcactaacct tatcgccttc ttttaccaag atggcagtaa
      901 tattgccttg catttgagca ttggcattaa tgctttgatt ggcatctact ttaccctgaa
      961 catctacata gtggttaaaa gtatctgtct ttattacaat ggtactgatg tttttaatgt
     1021 ttccgctaac ggtgctatcg cctgcttttt caagttcggt acgtagggca tcaatttttg
     1081 ccgacaactc gttttgttgc tttaataaat tttcgagttc tgtttttttg tcaaccgact
     1141 tattgccgca tgccgtaatg attgccaatg ccgcaatggc aacaattata tttattgatt
     1201 tcataaattt atgattgtaa tttttttaaa gaatccctgt tactatttaa ttaatcccaa
     1261 tgctttgtct aactcaacct tggctattaa agcatcataa accgtagtat aaaaaatact
     1321 ttcggctgcg cgttgttgcg attcggcatc tattacttcg agattagacc ccacaccctg
     1381 actgtatttt atttttgata cacgcacaat ttcattggcc agttcgcggt ttttcttttg
     1441 aatatcatac gaagctaaag cgttaataaa atttcgtttg gcagtattgt actctaactg
     1501 aaagccctct tgtaaagtaa tggtgctgtt gtcaatttta cgtaatgcca actcttcctg
     1561 cttaatctga ttgtgcctta caccactggt aaaaatgggc atggttaacc gtgcgcctac
     1621 ttgtgcattc caataccagc gtttatcact ttcaaataaa tcaaactcct cgctattggt
     1681 actggtgcct aagttaccat aagctgcaag cgtggggtag tat
//
LOCUS       DAOMAA010000488         1721 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_75590_length_1721_cov_2.429172, whole genome shotgun sequence.
ACCESSION   DAOMAA010000488 DAOMAA010000000
VERSION     DAOMAA010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75590_length_1721_cov_2.429172"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..1619
                     /gene="accC"
                     /locus_tag="PLO59_06950"
     CDS             120..1619
                     /gene="accC"
                     /locus_tag="PLO59_06950"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018478074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HQV00881.1"
                     /translation="MNNITKVSKISKVLVANRGEIAIRIMRSLHEMQIASVAVYSTAD
                     RNALHVRFAQQAFCIGEPASTQSYLRGDKIIEVAIEAGCDAIHPGYGFLSENAAFADA
                     VKAAGLIFIGPSAESMNKMGDKISAKNTAKSFNIPLVPGIDYAIRDIEEAKKIAHQIG
                     FPVLIKASAGGGGKGMRIVHDAASFEELLKLAQSEAMSSFNDDAVFIEKYVAKPRHIE
                     VQVLGDMYGNLVYLFERECSVQRRHQKVIEEAPSSILTPALRKKMGECAVQAARSCNY
                     YNAGTVEFLMDEQLNFYFLEMNTRLQVEHPVSEIITGLDLVKEQIKIAEGQELSFKQD
                     DLKINGHAIEVRVYAEDPLNQFLPDIGKLVTYKIPQGPGVRVDDAYEEGMDIPIYYDP
                     MIAKLITHAESRQAAIDKMIQAIDNYQISGVATTLGFCKFVMQHEAFVSGNFDTHFVP
                     LYFKPEVLTNNGTDNDIAVLMAAMVLSEKQQIIADEPYTATSNWKRNRQ"
BASE COUNT          549 a          302 c          363 g          507 t
ORIGIN      
        1 agcgcctgat tgtggttcag gaggtcgtgg gttcgagccc catcttccac ccaccttaat
       61 attaatccga aacaaatagt ttcggatttt ttttatcctt tttaaactgt aatgcgctta
      121 tgaataatat taccaaagtt tcgaaaataa gtaaggtgct cgttgccaac cgtggcgaaa
      181 tagccatacg tataatgcgt tcattacatg aaatgcaaat tgcctctgtt gccgtttatt
      241 caactgccga tcgcaatgcg ttacatgttc gatttgcaca gcaagcattt tgtattggcg
      301 aacctgcatc aacacaatca taccttcgtg gcgataaaat aatagaagta gcaattgaag
      361 ccggttgtga tgccatacat cccggttacg gttttttaag cgagaatgct gcatttgctg
      421 atgcagttaa agcagccgga cttatattta ttggtccatc agccgagtct atgaataaaa
      481 tgggcgataa aatatcagca aaaaatactg ctaagtcttt taatatacca ttagtgccgg
      541 gcattgacta tgcaataaga gatatagaag aagcaaaaaa aatagcccac caaattggtt
      601 ttccggtttt aattaaagcc agtgctggtg gaggcggcaa aggtatgcga attgtacacg
      661 atgctgcttc gtttgaagag ttgcttaaac ttgcacaaag cgaggcaatg agttcgttta
      721 atgacgatgc tgtttttata gaaaaatatg ttgccaaacc aaggcatatt gaagtacagg
      781 tattgggcga tatgtatggt aatttagttt acctgttcga gcgcgaatgt agtgtgcaac
      841 gcaggcacca aaaggttatt gaagaagcac catcatccat cttaaccccg gctttaagaa
      901 aaaaaatggg cgaatgtgct gtgcaggcag ctcgaagctg taattattat aatgcaggta
      961 cagttgagtt tttaatggat gagcaactca atttttattt tttagaaatg aatacccgtt
     1021 tgcaagttga acatccggta agcgaaataa ttaccggttt ggatttggta aaagagcaaa
     1081 taaaaattgc cgaagggcaa gaattatcat ttaaacaaga tgatttaaaa attaatgggc
     1141 atgctattga ggtgcgcgtg tatgccgaag acccattaaa ccaattcttg ccggatatcg
     1201 gaaaattagt tacctacaaa ataccacagg gaccgggtgt aagagtggac gatgcctacg
     1261 aagagggtat ggatattcct atctattatg atccaatgat tgccaaatta ataacacatg
     1321 ccgaatcgcg tcaagcggca attgataaaa tgattcaagc aattgataat tatcagattt
     1381 caggtgttgc aactacactt ggcttttgca aatttgtaat gcaacatgag gcttttgttt
     1441 caggcaattt cgatacgcat tttgttccct tatattttaa gcctgaagtg ttgaccaata
     1501 atggcactga taatgatatt gccgtgctta tggcagcaat ggtgttatca gaaaaacaac
     1561 aaataattgc tgatgaacct tatacggcaa caagcaattg gaaacgcaac aggcagtagg
     1621 ttactaatta ttgacaatta aaaacctgct gtctgttgtg cagttttaat ttttattttt
     1681 tgataaaagg aaactgaatt acaccttcat ctgaaacaac t
//
LOCUS       DAOMAA010000489         1720 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_75672_length_1720_cov_2.533333, whole genome shotgun sequence.
ACCESSION   DAOMAA010000489 DAOMAA010000000
VERSION     DAOMAA010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75672_length_1720_cov_2.533333"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="PLO59_06955"
     CDS             <1..365
                     /locus_tag="PLO59_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1736 domain-containing protein"
                     /protein_id="HQV00882.1"
                     /translation="ILKDNNAGPPSIADNSLMAAPNFLIQKCTAVVIMGMYAMLLIFP
                     HPLVFDYSFNQIPLSSVSDWRFLLAFILYAALLIIAIKGFAKKNPYAMVVIFFFIVSS
                     VSSNFLMVIGTNMAERLM"
     gene            474..1142
                     /locus_tag="PLO59_06960"
     CDS             474..1142
                     /locus_tag="PLO59_06960"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQV00883.1"
                     /translation="MLTATVCLVALLYSLKTFSYSRVWKDNITLYESGVVNAPLSTRT
                     HFYLGNAICKPDYYEKFPKPEQEKIIARGISELKKSAEIYPAFSEPWAQLGVAFYKKK
                     DLDSAMYFYNKALAVAPYLATVHNNIGTVYFETGKYNEAITAFSKATQFDANYSEAWC
                     NLGSAYGTLGQLDAAIQNFLTAVKCDPNNVMALKFLGITYQNKGDQYNAKMYFDMAAQ
                     AGAK"
     gene            complement(1495..>1720)
                     /locus_tag="PLO59_06965"
     CDS             complement(1495..>1720)
                     /locus_tag="PLO59_06965"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV00884.1"
                     /translation="TAYPNPSNGLVALKGLSGNGVVSVVNQMGQVVVTKSFSNQTSLG
                     LDLTAQPAGLYHVKVQNGNTISNLTINVNR"
BASE COUNT          565 a          295 c          287 g          573 t
ORIGIN      
        1 atattttaaa agacaataat gccggccctc catccatagc cgataattca ttaatggcag
       61 cacctaactt tttaattcaa aagtgtacag ctgttgtaat tatgggtatg tatgcaatgc
      121 ttttaatttt tccgcatcca ttagtttttg attattcgtt taatcaaata ccacttagca
      181 gcgtatctga ttggcggttt ttactagcat ttattttgta tgctgcatta cttataattg
      241 ctataaaagg gtttgctaaa aagaacccat atgcaatggt tgtaattttc ttctttattg
      301 tatcgtcagt atcatctaac tttttaatgg taattggtac caatatggcc gaacgattaa
      361 tgtagacacc ggcattggct tattgtatgg gtgttgcttt tttgttggat gcatttataa
      421 gtaaacccac aataaaagtt gaaagcatta ctgcattttt caaagctcga tacatgctta
      481 ctgcaactgt ttgtttagtg gcgctgttat acagtcttaa aactttttcc tacagcaggg
      541 tttggaaaga caatattacg ctttatgaaa gcggagtggt aaatgcacca ttaagtacgc
      601 gtacacactt ttatttgggt aatgcaattt gtaaacccga ttactatgaa aaatttccta
      661 aacccgaaca agaaaaaata attgcacgcg gtatcagtga attaaaaaaa tcggccgaaa
      721 tataccctgc ttttagtgag ccatgggcac aattaggcgt tgcattttat aagaaaaaag
      781 atttagacag tgccatgtat ttttacaaca aagcattggc tgttgctcca tatttagcta
      841 cagtacataa caatattggc accgtttatt tcgaaaccgg taagtataac gaagccatta
      901 ccgcatttag caaagccaca caatttgatg ctaactacag cgaagcatgg tgtaacctgg
      961 gtagtgctta tggtacattg ggtcagttag atgcagccat tcaaaatttt ttaactgccg
     1021 taaaatgcga ccctaataat gtgatggctt taaaattttt aggtattacc tatcaaaaca
     1081 aaggcgacca atacaatgct aaaatgtatt ttgatatggc agcacaagcg ggtgctaaat
     1141 aatttaagcc tgtccatttt attttacttc acgtagtaag atccaattaa aaacagctac
     1201 atgaattagc attattactt gcttaaacgg tatttaggta ttgttaactt gtattgaaac
     1261 atttagtaaa acaatgagat ttaaatataa tttccattta actatatcta tatctttaaa
     1321 aagctacggc tataaaaatt catacctgaa aagcctgcac tattatttta atagccaatc
     1381 aggcctgatt attttggaat ttttttatat gtataaatta tctgcttaag caccaaacaa
     1441 aaagaggcct tgcaaaaatg caagacctct ttcctgatta agtaaaccta agttttagcg
     1501 atttacatta attgttaaat tactaatggt gttaccattt tgaactttaa catgatataa
     1561 acctgcaggt tgagcagtta aatctaaacc taatgaagtt tgattgctga atgacttggt
     1621 tacaacaacc tgacccattt gatttacaac agaaaccaca ccgtttccac ttaatccttt
     1681 taaggctact aaaccattcg aaggattagg ataagcagta
//
LOCUS       DAOMAA010000490         1719 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_75755_length_1719_cov_2.171274, whole genome shotgun sequence.
ACCESSION   DAOMAA010000490 DAOMAA010000000
VERSION     DAOMAA010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75755_length_1719_cov_2.171274"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="PLO59_06970"
     CDS             complement(<1..701)
                     /locus_tag="PLO59_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017257278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HQV00885.1"
                     /translation="MSNRRNFLKQFSAGTALLSMGAFPLQAFAKRDIVKITILHTNDV
                     HSRVDPFPDNDPKFAGKGGVAQRAAIIHQIRNTERNVLLLDAGDMFQGTPYYNMFGGE
                     VEIKLMSLLGYDAGTIGNHDFDNGIDALAKQLIHANFPLINSNYDFKNTAMEGKTLPH
                     KIFEKDGIRIGVFGMGIELKGLVPDKLYGATIYYNPIESAAKQAHYLKKTQHCDLIIC
                     LSHLGYKYPDGKVSD"
     gene            complement(694..1422)
                     /locus_tag="PLO59_06975"
     CDS             complement(694..1422)
                     /locus_tag="PLO59_06975"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HQV00886.1"
                     /translation="MARFIFILLLAIVSCKPTSVVVKKELQQYEITKVNIDSNTQIVI
                     APYKTKMDAIMNEVIGHSQTVMEKNIPESTLGNFVADAILNQAKKHTTTPIDFVFLNN
                     GGLRSALPFGEITMGNVYSLMPFDNEVVLLTLSGAQTKELIQFIIDKGGVPVAGIRII
                     IKNKLPYQVMIGNQKMDESKTYNLLTSDYLANGGDKLEMLRMVNQRQNLNLLLRNLIV
                     DEFRQQEKSGIKVFPKLDNRISYE"
     gene            1525..>1719
                     /locus_tag="PLO59_06980"
     CDS             1525..>1719
                     /locus_tag="PLO59_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009184542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HQV00887.1"
                     /translation="MLQFTNKIRVRYAETDKMGFVYYGNYATYFEVARVEFLRSMGLS
                     YKSVEDSGIIMPVLEFGPNAP"
BASE COUNT          508 a          307 c          300 g          604 t
ORIGIN      
        1 tcatcactta ctttgccgtc agggtattta taccccaaat gcgataaaca aataattaaa
       61 tcgcagtgct gtgttttttt taaatagtgt gcttgttttg ctgcgctttc aatagggttg
      121 taataaatag ttgctccata tagtttatcg ggtaccaagc cttttaattc aatacccatt
      181 ccgaaaacgc caattctgat tccatctttt tcaaaaattt tatggggcaa tgttttacct
      241 tccattgcag tattcttaaa atcataattc gaatttatta gtggaaaatt tgcatgtatc
      301 aattgtttag ccaatgcatc aatgccgtta tcaaaatcgt ggttaccaat agtaccggca
      361 tcatatccca acaaagacat gagtttaatt tctacctcgc caccaaacat attataataa
      421 ggcgtgcctt gaaacatatc accggcatcg agcagtagta cattgcgttc ggtatttctt
      481 atttgatgta tgatagctgc acgttgtgca acaccacctt tacctgcaaa ctttgggtca
      541 ttatccggaa atggatcaac acggctgtga acatcgttgg tgtgtagtat ggtaattttt
      601 acaatgtctc tttttgcaaa tgcttgcaat gggaaagctc ccatactgag taaggcagta
      661 cctgccgaaa attgttttaa aaaatttctt ctgttactca taacttattc tgttatctaa
      721 tttcggaaat actttaatgc ctgatttttc ctgttgccta aattcatcaa caatcaaatt
      781 gcgtaacaac aggttcaggt tttgtctttg attaaccata cgcaacattt ccaatttgtc
      841 gccaccgttt gctaaatagt cgctggttaa taaattataa gttttgcttt catccatttt
      901 ttgattgcca atcataacct gatagggaag tttgtttttt ataattattc gtatgcctgc
      961 aaccggtaca cctcctttgt ctatgataaa ttgaatgagc tcttttgttt gtgcacctga
     1021 taatgtaagt aaaactactt cgttgtcaaa tggcattaag ctataaacat tacccatagt
     1081 aatttcgcca aaaggtagag cgcttcgtaa acctccgtta tttaaaaaaa caaaatcaat
     1141 gggcgtagta gtgtgctttt tagcttgatt taaaatagca tcagccacaa aatttcccaa
     1201 tgtactttca ggaatatttt tttccattac cgtttggctg tggccaatta cttcattcat
     1261 aattgcatcc atttttgttt tataaggtgc aatcacaatt tgtgtattac tatcaatatt
     1321 taccttggta atttcatact gttgcagttc cttttttaca accacagaag ttggcttaca
     1381 tgaaacaatt gcaagtagca gtatgaaaat aaaacgggcc attatgattt taatttgtgt
     1441 gagcaagcta cacaaattgc cataagaatg atgttaaaaa ttattcgaat tgttttattg
     1501 ttgcagtaaa ttaaactgaa atatatgtta caatttacga ataagattag agtacggtat
     1561 gccgaaacag ataaaatggg atttgtgtat tatggaaatt atgcaacata ttttgaggtg
     1621 gctcgtgttg agtttttgcg aagcatgggg ctttcgtata agtcagtgga agatagtggt
     1681 attataatgc cggttttgga gttcggcccc aacgcgccc
//
LOCUS       DAOMAA010000491         1716 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_75973_length_1716_cov_3.074654, whole genome shotgun sequence.
ACCESSION   DAOMAA010000491 DAOMAA010000000
VERSION     DAOMAA010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75973_length_1716_cov_3.074654"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="PLO59_06985"
     CDS             complement(<1..327)
                     /locus_tag="PLO59_06985"
                     /inference="COORDINATES: protein motif:HMM:NF026121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4476 domain-containing protein"
                     /protein_id="HQV00888.1"
                     /translation="MKKIFVLIFALHAICYAQTPCSQPIEGTEFNKHFDVLNRIQNDQ
                     DRLATAKDLFRNKCLSAKQLKIVCMIFNNERPRLNFALYAFDRVADKDEFYEVLDAFK
                     LYSSAFK"
     gene            complement(330..>1716)
                     /locus_tag="PLO59_06990"
     CDS             complement(330..>1716)
                     /locus_tag="PLO59_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HQV00889.1"
                     /translation="NITMEETAAYSVKLAQRVGNELQIPVYLYEASQPDAKRNNLSVI
                     RAGEYEGFAKKITEPDWKPDFGPQTFNIRSGATVIGARDFLIAYNVNLNTKSVKRANS
                     IAFDIREAGRVKTENGKKVLDAHGNEIRIPGLLKGVKAIGWYIDEYKQAQVSINITRF
                     RETPLHVVFDACVNAAHQRGYRVTGSELVGLVPLSAMLDAGRYFLNKQKLSSGVSDDE
                     LIHIAVKSMGLDELAPFNPKQRIIEFSMAQNKRPLVNMNLEAFANETASDSPAPGGGS
                     ISAYVATLGVSLGIMVANLSASKTGWESTWQSFSDWAEKGQLLKKQLLDLVDEDTAAY
                     NKILDAFQLPKATDEEKQIRKAAVQAATRYAIEVPARVMEVSYSCFDLIAEMVKKGNP
                     NSVTDAAVGALCIRTAVMGAHLNVQINTVGFADKDFVNAVMHKCQVIADNTQLRESEI
                     LAATQNIINKA"
BASE COUNT          460 a          357 c          332 g          567 t
ORIGIN      
        1 tttaaaagcc gatgagtaca atttaaaagc atccagtact tcgtaaaatt cgtctttatc
       61 agctacacgg tcaaatgcat ataatgcaaa atttaaacgc ggacgttcat tattaaaaat
      121 catgcaaaca atttttaatt gtttggcact taaacattta ttacggaaca aatctttggc
      181 agtggctaat ctgtcctggt cattttgtat gcggtttaaa acatcaaaat gcttattaaa
      241 ttcagtgcct tctatgggtt ggctgcaagg tgtttgggca taacatattg catgcaatgc
      301 aaaaattaga acaaatattt ttttcatttt caggctttat ttataatgtt ttgagtggct
      361 gctaatattt cggattcgcg gagttgggta ttgtcagcta ttacttgaca tttatgcata
      421 actgcattca caaaatcttt atcggcaaag cctactgtat taatttgcac gttaagatgt
      481 gcacccatta cggctgttct gatgcagagt gcacccacgg cagcatcggt aactgaattt
      541 gggtttcctt ttttaaccat ttcggcaatc aagtcgaaac aactatagct cacttccatt
      601 acacgtgcag gcacttcgat agcataacgt gttgcagctt gaactgccgc ttttctaatt
      661 tgcttttctt catccgtagc tttgggtaat tgaaatgcat ctaaaatttt attgtaggct
      721 gctgtatcct catcaactaa atcaagcaat tgctttttga gcaactgacc tttttcggcc
      781 caatcagaaa acgattgcca ggttgattcc catccggttt tagatgcaga taagttagcc
      841 accataatgc ctaatgaaac acccagtgtt gctacataag ccgagatgct gccaccaccc
      901 ggtgcaggcg agtcgcttgc ggtttcgtta gcaaatgctt ccagattcat gtttaccagt
      961 gggcgtttgt tttgcgccat gctaaactct ataatgcgtt gtttgggatt gaatggcgcc
     1021 aactcatcta agcccattga cttaactgct atgtgtatta actcatcatc gcttacacct
     1081 gatgatagtt tttgcttgtt taaaaaatat cgtccggcat caagcatggc gcttaatggt
     1141 accaagccta ccaactcact gccggttaca cgatacccac gttggtgggc tgcattaacg
     1201 caggcatcaa atacaacatg caaaggtgtt tcgcgaaaac gtgttatgtt tatagatacc
     1261 tgtgcttgtt tgtattcgtc aatataccag ccaatagctt taacgccttt aagtaaaccg
     1321 ggtatgcgaa tttcattgcc atgtgcatct aaaacttttt taccgttttc ggttttaaca
     1381 cgtccggctt cgcgtatatc aaatgcaatt gaattggcgc gctttaccga tttggtattt
     1441 aggtttacgt tgtatgcaat taaaaaatcg cgtgcgccaa ttacagtagc gccacttctt
     1501 atattaaatg tttgcggacc aaaatcgggt ttccaatcag gctctgtaat tttttttgca
     1561 aagccctcat attcgcctgc acgtataacg gaaaggttat ttcgttttgc atcaggctgc
     1621 gatgcttcat aaagatatac cggaatttgt aactcgttgc ctacacgttg tgctaacttt
     1681 acactatagg ctgcggtttc ttccatggtg atattt
//
LOCUS       DAOMAA010000492         1714 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_76167_length_1714_cov_2.587703, whole genome shotgun sequence.
ACCESSION   DAOMAA010000492 DAOMAA010000000
VERSION     DAOMAA010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76167_length_1714_cov_2.587703"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1714)
                     /locus_tag="PLO59_06995"
     CDS             complement(<1..>1714)
                     /locus_tag="PLO59_06995"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV00890.1"
                     /translation="NANPGGTFAMGSTPDCVRYFTVSSTANGSSTNPTSRDSYTHLGG
                     SITNFGDIVCYDFTMNSPGQVITRLTGPGFKDWNINGTLLVNAGQIYFGAYNVFYGVA
                     DYGLTTINNIVMTGGELNLDYTTSTTLVLGNITLTGGLFYLGDRTFAGHGDLELRGNW
                     FQSTGFFGINHKTVYFRGNTLQTIAGNGTLFPYVEINNAAGVTILSGNQTIQDTLYLL
                     NGMLTLGNNQLTITGLNGQTPTSTGGITYTNPSSFIVTDGNGVLRQNDIGTARTGSIL
                     FPIGNSSTSYRPLTLNNSGTNDNFAARVFNNVYSGGNQGVGTTFTTNYVNRTWDVSET
                     VAGGSTLTATFEYAAVDELVLFNRNLCGVATHNGSAWVLTQTTAPVAGSNPYTRTTTV
                     NTTGHFTITSDLIPLPIQWLSFEVTKQQTNATLQWAVLNQNNASHYEIQRSLTGNLFE
                     PIGIVTANPNGYYTYSDNISKRSNEIIYYRIKQYDFNGNYTYSMVKSIANKLQSPLLI
                     FPNPAYDVLAIKHPKIGQAALTVYNSNGQLCYSLQSVFDSDQTEIKINHLISGLYTIQ
                     LTTQS"
BASE COUNT          470 a          333 c          377 g          534 t
ORIGIN      
        1 ctgattgcgt agtaagttgt atggtatata aaccgcttat taagtggttg attttaattt
       61 cagtttgatc agaatcaaaa acgctttgta gtgaataaca aagttggcca ttgctgttat
      121 aaacggttaa agcagcctgc ccaattttgg gatgtttaat ggccagcaca tcataagccg
      181 gattgggaaa tattaaaagc gggctttgaa gtttgttcgc aattgattta accatgctgt
      241 atgtatagtt gccattaaag tcgtactgtt taatgcgata gtaaattatt tcattcgagc
      301 gtttgcttat gttgtcggaa taggtgtaat aaccgtttgg gtttgccgta actatcccaa
      361 taggttcaaa caaatttccg gttaagcttc tttgtatctc ataatgactt gcattatttt
      421 ggtttagaac agcccattgt aatgtagcat tggtttgctg tttggttact tcaaatgata
      481 gccattggat tggtagtgga attaaatcgc tggtaatggt aaagtggccg gtagtattta
      541 cagtagttgt tcgtgtatat ggattactgc ctgcaaccgg cgctgttgtt tgcgttaaaa
      601 cccaggcact gccattgtgt gtggctacac cacataggtt acggttaaaa agcaccagtt
      661 catcaacagc agcatactcg aaagttgcag ttaatgttga acctccggct actgtttcgc
      721 ttacgtccca ggtgcggtta acatagtttg ttgtaaaagt agtgcctacc ccttgattgc
      781 ctccactgta aacattatta aacacgcgtg ctgcaaaatt atcattggtg ccgctattgt
      841 ttaaagttaa aggacggtaa ctggtactgc tattgccaat cggaaataat attgaaccgg
      901 tacgcgcggt gccaatatcg ttctgcctta aaacgccatt accatcagtt acaataaatg
      961 agctggggtt ggtgtaagta atacctccgg ttgatgtggg tgtttgaccg tttaagcctg
     1021 taatagtgag ctgattattg cccaatgtga gcataccatt tagcaggtac aaagtatctt
     1081 gtatggtttg attgcccgat agtatggtaa cccctgctgc attattaatt tcgacatacg
     1141 gaaacagtgt gccatttccg gcaatggttt gcagtgtgtt gcctctaaag taaacggttt
     1201 tgtggtttat gccgaaaaag cccgtgcttt gaaaccagtt gccgcgcaat tctaaatcgc
     1261 catgccctgc aaatgttctg tcgcccaaat aaaataggcc gcctgttaaa gtaatgtttc
     1321 ctaaaaccag ggtggttgag gtagtgtagt ctaagtttaa ttcgccaccg gtcattacaa
     1381 tgttattaat ggtagtaagt ccataatcgg caaccccata aaaaacgttg taagccccaa
     1441 aatatatttg gcctgcattt accagcaagg tgccattaat gttccaatct ttaaaccccg
     1501 gccctgttaa acgagtaata acctggccgg gagaattcat tgtaaaatcg tagcaaacaa
     1561 tatcgccaaa gtttgtaata ctacccccta agtgcgtata actatcgcgc gatgtagggt
     1621 ttgtactact gccatttgct gtactgctaa ctgtaaaata acgcacacaa tcaggcgtgc
     1681 tgcccatagc aaaagtacct cctgggttgg catt
//
LOCUS       DAOMAA010000493         1712 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_76359_length_1712_cov_2.174412, whole genome shotgun sequence.
ACCESSION   DAOMAA010000493 DAOMAA010000000
VERSION     DAOMAA010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76359_length_1712_cov_2.174412"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1712)
                     /locus_tag="PLO59_07000"
     CDS             complement(<1..>1712)
                     /locus_tag="PLO59_07000"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HQV00891.1"
                     /translation="GTNVASLSGSKTGAGSSVTWTTNGTGTFNNVNSLTPNYTLSAAD
                     KAQGFVTLIITTNDPAGPCGAKSDAVSVYYREAVADAGPTELCGDTIWTVYGSVATSI
                     YTPIAVQWTSSGTGTFNSPNSYGATYYASAADKAAGSVTLKLTPSDPTGLCNNYVSDS
                     IKIWFNPHYTVNAGPDSLFYCGDKQVQLAGTRTSPTSVWSTSGTGTFNNPANLNAKYN
                     FSTADTIAGKVVLRLQSNDPAGPCGRVMDSVIVIIREVPHATAGTNSPVCLGTNLNIT
                     ASGAGNYSWSGPSGYSSTIQNIIRPNATLAMAGNYTVVVTNSFGCSSTATTTATIISC
                     GCIQPIVLTGKTDATCKGASNGTAATTINNVAGPFTYLWSHGATTQNVTGLSAGVYTV
                     TVSSAPNCSATASVTINEPDNIVITPNIINATCGNANGSAQAVVTGGTPGFTYAWNTV
                     PAQFSSVISSMLPGTYTVTVTDANGCTKSQSATIVNNGSAITEVTVQIKRPCFDDNNG
                     KLTAKTITGGTAPFTYLWSTGAVTSSINQLTPGNYTVTVTDANGCNFIQSTTLIQRSE
                     IIDH"
BASE COUNT          490 a          353 c          352 g          517 t
ORIGIN      
        1 catgatcaat aatctcactg cgttggatta aagtagttga ttgtataaaa ttacaaccat
       61 tagcatcggt aacagttacg gtataattac cgggcgttaa ctgatttatt gaagatgtaa
      121 ctgcaccggt actccataag tatgtaaatg gagccgtacc acctgtaatg gtttttgccg
      181 taagcttacc attgttgtca tcaaagcaag gacgttttat ttgtactgta acttctgtta
      241 tagcactacc gttattaacg atagttgcac tctggctttt cgtacaacca ttagcatcag
      301 taacagttac tgtataggtg ccaggcagca tgctgcttat tacacttgaa aattgtgcag
      361 gtacggtatt ccaggcataa gtaaatccgg gtgtaccacc ggtaacaact gcctgagcgc
      421 tgccattggc attgccacag gtagcattaa taatgttagg ggtaataact atattatctg
      481 gttcgtttat ggttacactt gcagttgccg aacaattggg tgccgatgaa acggttactg
      541 tataaacacc tgcagataaa ccggtaacat tttgtgtggt tgcaccatgg ctccataaat
      601 aggtaaatgg accagcaaca ttatttatgg ttgttgccgc tgtaccattg gaagcacctt
      661 tacatgtggc atcggtttta ccggttaata caattggctg aatacaaccg caacttataa
      721 tggtagctgt agtagtagct gttgatgaac agccaaaact attcgtaaca actactgtat
      781 aatttccggc catggccaag gttgcatttg gtcttataat attttgaatg gtagatgaat
      841 aaccacttgg tccactccaa ctataattgc ccgcaccact tgctgttatg tttaaattag
      901 tacccaaaca aaccggtgaa ttggtaccgg ctgttgcatg cggcacttct ctgattatta
      961 caattacact atccataaca cggccacaag gccctgcagg atcattgctt tgtaagcgca
     1021 atactacttt gcctgcaatg gtatcagctg ttgagaaatt atactttgca tttaaatttg
     1081 caggattatt aaaagtaccg gtaccactgg tactccatac actggttggt gaggtgcgcg
     1141 taccggctaa ttgtacttgt ttatcaccac aataaaataa actatcgggg cctgcattaa
     1201 ccgtataatg tgggttaaac caaattttta tcgaatcgct aacataatta ttacataacc
     1261 ctgtaggatc gctcggtgta agttttaaag ttacacttcc ggcagcttta tcagcagccg
     1321 atgcataata agttgcaccg tatgaatttg ggctattaaa ggttccggta cctgatgatg
     1381 tccattgtac agcaatgggt gtgtaaatag atgtagcaac agagccgtaa actgtccaga
     1441 tagtatcacc acaaagttca gttggtcctg catcggcaac tgcctcacga taataaacgc
     1501 taaccgcatc cgactttgca ccacatgggc cggcaggatc gtttgtagtt ataataagtg
     1561 ttacaaatcc ctgagcttta tcagcagcac ttaatgtgta atttggagtt aaactgttta
     1621 cattattaaa agtacctgta ccattagtag tccatgtaac acttgaaccg gcacctgttt
     1681 tactgccgct taacgatgca acattggtac cg
//
LOCUS       DAOMAA010000494         1711 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_76428_length_1711_cov_2.803744, whole genome shotgun sequence.
ACCESSION   DAOMAA010000494 DAOMAA010000000
VERSION     DAOMAA010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76428_length_1711_cov_2.803744"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="PLO59_07005"
     CDS             complement(<1..595)
                     /locus_tag="PLO59_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00892.1"
                     /translation="MDKTVLIIAYYFPPVKGAAPWRPYSWCQYFGQQQLKTTVITRHW
                     QGFESNWAQMTAPNFEPPKVGIEKDYTLIQLPTKAWCLYTFYRNYLFKIPGLSALFFA
                     FAAALGRFGLEVDAYTTFAAYLINHLKTNNYDAIVVTYPPSNLLKLLPLLRKHSRAKI
                     IVDFRDWLNNEMLQHNYKPGIKQQYLNYCQSCYAKKHL"
     gene            735..>1711
                     /locus_tag="PLO59_07010"
     CDS             735..>1711
                     /locus_tag="PLO59_07010"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015809083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HQV00893.1"
                     /translation="MPQYYSAGNIPPKRHTQFRKPNGQLYAEELVSTHGFSNVYSLIY
                     HCYPPTMVKSVGEGVDISPALAFKRSLKHQSFIGYNVKPQSDYLKSRTPVLVNSDLHI
                     SLAAPQQSNTDYFFKNADADEMIFIHEGSGRLDTIYGSLDFAYGDYLIIPRGTIYQMH
                     FNDSNNRLLIVDSFSPIETPRRYRNAYGQYEEHAPYCERDFRKPKDLKTFDQTGDFKV
                     IIKKEQMLYDYVYATHPFDAIGWDGNHYPYAFSIHDFEPITGRIHMPPPIHQTFEAHN
                     YVICSFVPRLYDYHPLSIPVPYNHSNVDSDEVLYYVDGDFMSRKSVVRG"
BASE COUNT          538 a          337 c          321 g          515 t
ORIGIN      
        1 ttaaatgctt tttagcataa caactctgac agtagtttaa atattgctgc tttatgcccg
       61 gcttataatt gtgctgaagc atttcattgt taagccaatc tctaaaatca acaataattt
      121 ttgcccggct atgctttcgt aaaagcggta atagttttaa taaattactt ggcgggtaag
      181 taacaacaat ggcatcgtag ttattagttt ttaaatgatt tataaggtaa gctgcaaaag
      241 tagtgtaagc atctacctct aaaccaaagc gacctaatgc tgcggcaaag gcaaaaaaca
      301 aggcgctcaa acccggtatt ttaaataaat agttacggta aaaagtatat aaacaccatg
      361 ctttggttgg taactgaata agtgtataat ctttttctat gcctaccttt gggggctcaa
      421 aattgggcgc tgtcatttgc gcccaattac tttcaaaacc ttgccagtgt ctggtaatta
      481 cagttgtttt taattgttgt tggccaaaat actggcacca cgaatacgga cgccatggtg
      541 cagcaccctt tacaggcgga aaataatagg caataattaa aacggtttta tccacacgta
      601 gtttctctca aataaggcgg ctaaattaaa aagcaattgc aacaagcatc catgttcttt
      661 aattcaaatt tttatcggat aaaaaaacat tgacaataaa aacccattaa atttgcacta
      721 taataaaaat cgttatgcca caatattact cggccggcaa cataccgcct aagcgccata
      781 cacaatttcg gaaacccaat ggccagctat atgccgaaga gttggtttca acacatgggt
      841 ttagtaatgt ttattcgtta atttatcact gctatccgcc caccatggta aaatcagttg
      901 gcgagggtgt tgatatttcg cctgcattgg cctttaaacg ctcgcttaaa catcagagtt
      961 ttattgggta taacgtaaag ccgcaatccg actacctaaa aagccggacg cccgttttag
     1021 ttaacagcga tttgcatatt agcctggcag caccacagca atcaaacacc gactattttt
     1081 ttaaaaatgc cgatgccgat gaaatgattt ttatacatga aggcagtggc aggttagata
     1141 ctatttatgg aagtttggat tttgcttatg gcgactattt aatcattcca agaggtacca
     1201 tttatcagat gcattttaat gatagcaata acaggctatt gatagttgat tcgtttagtc
     1261 ccattgaaac acccaggcgg tatcgtaatg cttatgggca atacgaagag catgcgcctt
     1321 attgcgaacg cgatttcaga aaaccaaaag atttaaaaac ttttgaccaa acaggcgatt
     1381 ttaaagtgat tataaaaaag gaacaaatgc tgtatgatta tgtatatgcc acacatcctt
     1441 ttgatgccat tggatgggac ggcaaccatt acccttatgc atttagcatt catgattttg
     1501 agcccataac cggacgcata cacatgcctc cgcccataca tcaaactttt gaagcccata
     1561 attatgtgat ttgctccttt gtgccacgtt tgtacgatta tcatccgtta agcatacccg
     1621 taccatataa ccacagtaat gtagatagcg atgaagtttt gtattacgtt gatggcgatt
     1681 ttatgagccg caaatcagtt gtgcgtggtc a
//
LOCUS       DAOMAA010000495         1709 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_76590_length_1709_cov_2.908706, whole genome shotgun sequence.
ACCESSION   DAOMAA010000495 DAOMAA010000000
VERSION     DAOMAA010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76590_length_1709_cov_2.908706"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="PLO59_07015"
     CDS             <1..554
                     /locus_tag="PLO59_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HQV00894.1"
                     /translation="DAERYQTVYAKHEGAVAAPTAGLHFSKQLIKRLELKNVKFAEVT
                     LHVGLGTFRPVEVEDLTKHKMDSEQFEITQESADIVNAGKARKKRICAIGTTSMRAIE
                     SSVTTSGELKPAEGWTNKFIFPPHDFAVANCMITNFHMSESTLLMMTCAFGGYENIMN
                     AYKVAVKEKYRFYSYGDAMLIID"
     gene            745..1491
                     /locus_tag="PLO59_07020"
     CDS             745..1491
                     /locus_tag="PLO59_07020"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HQV00895.1"
                     /translation="MNNYIKALIVAIGMVASAFIIGKAIERFKTEDRYISVKGFSEKE
                     VKADLVIWAIKIKVVNNDLKDGNTALAAAKSKVVNFLTNKGVQPTEINTLDMMVIDHE
                     AAEYGNNHLNKMRYIIEETIEVRSNHVDLIQSISRMTNELLNAGVALSTKSDWNGTGL
                     QFMFTKLNTIKPQMITEAIRNAKDAAIQFTNESNTKLGKLRKANQGLFSIQDRDQTFS
                     AAVDDGYVNNGKSGVMKKVRVVVSVDYSVE"
BASE COUNT          590 a          324 c          330 g          465 t
ORIGIN      
        1 aagatgccga acgttaccaa accgtatatg ccaaacacga aggtgctgtg gctgcgccaa
       61 cagcaggctt gcactttagc aaacaactga taaaacgatt agaacttaaa aacgtaaaat
      121 ttgccgaagt aaccttacat gttggtttag gtacgtttcg tccggtagaa gtagaagacc
      181 ttaccaaaca caaaatggat agcgagcagt ttgaaattac acaagaaagc gctgatattg
      241 ttaatgcggg caaggcacga aaaaaacgaa tttgtgctat tggtactacc agtatgcgtg
      301 caatcgaatc gagcgtaacc acatcgggcg aattaaaacc ggccgaaggt tggactaata
      361 aatttatttt cccgccacat gattttgctg ttgccaattg catgattacc aatttccaca
      421 tgtcagaatc aacactgctg atgatgactt gtgcctttgg cggatacgaa aatattatga
      481 atgcttacaa agtagccgta aaagaaaaat accgctttta tagttatggc gatgccatgc
      541 ttattattga ttaggcaccg gtttactgct atgtaaataa tcctgatgta atggtggttt
      601 gaaaagcaac caaattgcaa tcgggatttt ttttgcttaa gtgtaataat cgaaaataca
      661 agaacactgt tttaaatgat gtccatttgt aacatcaatc ataaatgttt atttgtaaat
      721 tatttataac ttataaaaca aatgatgaat aattacatca aagcgttaat cgttgccata
      781 ggcatggtgg caagcgcatt tataattgga aaagccattg agcgctttaa aaccgaagac
      841 cgatacatat ccgtaaaagg attttcggag aaagaagtaa aagccgactt ggtaatttgg
      901 gctattaaaa ttaaagtagt taataacgac cttaaagacg gcaatactgc tttagcggca
      961 gctaaaagca aagtagttaa ctttttaacc aataaagggg tacaacccac tgaaattaac
     1021 acattagaca tgatggttat tgaccatgaa gcggcagagt acggtaacaa ccatttaaat
     1081 aaaatgcgct acattattga agaaaccatt gaagtacgca gcaaccatgt tgaccttata
     1141 caaagcatta gccgtatgac taatgagctg ttaaatgccg gtgttgcact gtcaaccaaa
     1201 agcgattgga atggaacagg cttgcagttt atgtttacca agctaaatac gataaagccg
     1261 caaatgataa ccgaagctat tagaaatgca aaagatgctg ccatacaatt tacaaacgaa
     1321 agcaatacca agttgggcaa acttcgtaaa gccaaccagg gtttgttttc gatacaagac
     1381 cgcgaccaaa cttttagcgc tgccgttgac gatggttatg taaacaatgg aaaatcgggc
     1441 gtgatgaaaa aagtacgtgt tgtagtttct gttgattact cagttgaata gttataaagg
     1501 gtttaaaata gcgtgccaaa gcgcactttc acacataaat gtaggcacgc taccttgcgc
     1561 taacaaatag ttgagtatgc ttaaataact gtgtacaaac catttcacta atcacatctt
     1621 acctctcacc tctcacattt cacctctcac atttcaccgc tcactcctta acaaacctgc
     1681 cataatacat tttatcgcct tgctgtaaa
//
LOCUS       DAOMAA010000496         1706 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_76823_length_1706_cov_3.419140, whole genome shotgun sequence.
ACCESSION   DAOMAA010000496 DAOMAA010000000
VERSION     DAOMAA010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76823_length_1706_cov_3.419140"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="PLO59_07025"
     CDS             complement(<1..396)
                     /locus_tag="PLO59_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HQV00896.1"
                     /translation="MIANILLILCGVVLIAYLIDVFSAKIKVPSVVLLLTFGFAIKYS
                     LQLLNIAIPDVSRILPLVGTVGLVLIVLEGALELKIDATKIPLIKKAFWVSWLAILIL
                     AVITVSWCYYVFNYSINDSIVNLMPLCIIS"
     gene            complement(442..1701)
                     /locus_tag="PLO59_07030"
     CDS             complement(442..1701)
                     /locus_tag="PLO59_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HQV00897.1"
                     /translation="MKSLKIIIALLVVSFKIQASYILIPMDESQKNHLKAYGIAYWVL
                     TQEVDINWLLNYRGGSFMIKQISAIENECKVRGVSYTIIADGQADAIIQEIANPEVNM
                     DVVKLEKAPRIAVYTPKNKLPWDDAVTMVLKYAEIKYDIVYDEEIITGKLPLYDWLHL
                     HHEDFTGQYGKFYSMYHNAPWYQEQVRTNEAMAKKLGFSKVSQLKLGVAKKIEEFTIG
                     GGFLFTMCSGTDSYDIALAAQDLDIADKIYDGDGTTINYNNKLDFAKTFAFENFKLST
                     NPVEYEFSNIDVNPQQRGVSRDNDVFTLFEFSAKWDPVPTMLCQNHEQVIAGFMGQTT
                     AFDSRYIKSNVIVMGENKSGGFAKYIHGEHGKGTWTFYGGHDPEDYQHMVGDPPTDLN
                     LHPNSPGFRLILNNVLFPAAKKKKQKT"
BASE COUNT          513 a          336 c          309 g          548 t
ORIGIN      
        1 gctaataatg caaagtggca tcaggttaac tatactatca ttaatggagt aattgaaaac
       61 atagtaacac caacttacgg taattactgc taaaattaat atagcaagcc atgacaccca
      121 aaatgctttt tttattaatg gtattttggt tgcatcaatt ttaagttcca acgcaccttc
      181 gagcacaatt aaaaccaatc caacggtacc tactaaaggt aaaatacgtg atacatcggg
      241 tatggcaata tttaataact gaaggctata tttaattgca aaaccaaaag ttaatagcag
      301 caccaccgat ggcactttaa ttttagccga aaaaacatca atcaggtatg caattaaaac
      361 tacaccacat aaaatcagta atatgttagc aatcataata tagttttaaa gtgccggcca
      421 atactattac agatgttgaa attaagtttt ttgttttttc tttttggcag ccggaaacaa
      481 tacattattt aaaattaaac gaaagcccgg tgaatttgga tgcaggttta aatcggttgg
      541 cgggtcgcct accatgtgtt gataatcttc cggatcgtgt ccgccataaa atgtccaggt
      601 gcctttgcca tgttcgccat gtatgtattt ggcaaaaccc cctgatttgt tttcgcccat
      661 cacaattaca ttgctcttaa tataacgact atcaaaagcc gttgtttgtc ccataaaacc
      721 agcaataact tgttcgtggt tttggcataa catggtgggt accggatccc atttagccga
      781 aaactcaaaa agtgtaaaca catcgttatc gcgcgatacg ccacgttgtt gcgggttaac
      841 atcaatgttg ctaaactcat actcaaccgg attggtactc agtttaaagt tttcgaaagc
      901 aaaagttttt gcaaaatcta atttgttgtt gtagttgatg gtggttccat cgccatcata
      961 aattttatcg gcaatgtcaa ggtcttgcgc tgccaatgca atgtcatacg agtcggtgcc
     1021 tgagcacata gtaaataaaa agcctccacc aattgtaaac tcttcaattt tttttgcaac
     1081 acccaattta agctgtgata ctttgctaaa gcctaatttt tttgccatag cttcgttagt
     1141 gcgcacttgt tcctgatacc aaggagcgtt atggtacata gaataaaact tgccgtattg
     1201 gcctgtaaaa tcttcgtgat ggaggtgcag ccaatcgtac agaggaagtt taccggttat
     1261 tatttcctca tcatacacta tgtcatattt tatctcggca tattttaata ccatggttac
     1321 ggcatcgtcc cagggtaatt tgtttttagg cgtgtaaact gcaatgcgtg gggccttttc
     1381 taatttcacc acatccatat ttacttctgg gtttgcaatt tcctgaatta tggcatcggc
     1441 ctggccatcg gcaataatgg tgtaggatac accgcgtacc ttacattcgt tttctatggc
     1501 cgaaatttgt ttaatcataa aactaccgcc acggtaattg agcaaccagt ttatatctac
     1561 ctcttgtgta agtacccaat aagcaatgcc gtatgcttta aggtgatttt tttgagattc
     1621 gtccataggt ataagtatgt acgatgcctg tattttaaag ctaacaacaa gtaaagcgat
     1681 gataattttt aaactcttca tttttt
//
LOCUS       DAOMAA010000497         1704 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_76998_length_1704_cov_3.152820, whole genome shotgun sequence.
ACCESSION   DAOMAA010000497 DAOMAA010000000
VERSION     DAOMAA010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76998_length_1704_cov_3.152820"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(254..327)
                     /locus_tag="PLO59_07035"
     tRNA            complement(254..327)
                     /locus_tag="PLO59_07035"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(293..295),aa:Cys,seq:gca)
     gene            874..>1704
                     /locus_tag="PLO59_07040"
     CDS             874..>1704
                     /locus_tag="PLO59_07040"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HQV00898.1"
                     /translation="MSWLLFLSLKSVYASDNKQIITISTQITHNACAGGKDGSIDISV
                     SGGLAPYSFLWSQGNTTEDLLFVESGTYTVTVTDDAGIVQTAVATITEPSPVVINLVN
                     LSNVTCYSAGNGSINISPSGGVSPYTYLWNNGSTVEDRVSLAPGLYTVLVTDDNGCST
                     NETYQITGPSLLTCNVSTVKAISCFGLSNGSAVINTNSGGTQPYTYTWSSGQTTQTAT
                     GLAAGLTLVTVTDANGCNSICGINMQQPTLLVANYTQSNVTTTGGSNGSIDVTVSGGS
                     Q"
BASE COUNT          556 a          315 c          319 g          514 t
ORIGIN      
        1 tacgattaac gaaaaaggcc caggcagtat tttaatttat ggcagccaat tatcaactgg
       61 catttacacc tacacattaa ttgccgatgg caaacccgtt gaaaccaaac gtatggtaaa
      121 ggtgaagtaa ttaaaatgag cgtttcagtt gcgtgcagaa accaacatga agcaaacaac
      181 tttgctttaa ataaaaattt gtttacggat tataataaaa aagggctatc cacagcggac
      241 agcccttttt aattggaggt cgcgagcaga ttcgaactgc tgtacaaggt tttgcagacc
      301 tctgcctagc cactcggcca cgcgaccatt ttttgagtgc gcgcaaaatt aacaaaattt
      361 cattcccaca aactttttgt ttttcaagtt tcgcaaatag tatttcaatt tttgggaata
      421 ctataagtac catcataaca atgatactgt acgatgcctt tttaagaaat cttataaagt
      481 ttgattaagt atttgcaaga cagattagtt gctaacaagc tattaattaa tgtttaaaag
      541 gatggtttta ttaacattaa ttaacctgtt attcaggtat tttagttaaa aaatatatag
      601 ttatgtattg aaaatcaata agataataac tatgtgaaaa agtcaatagt tagctgctta
      661 ttgttaaaaa atagtgcttc tacattcgcc cttgttatgg ctttacatga atgtatctcg
      721 aatcataagc tttttaaacc atcaacttaa aagcttacgt aacgaattaa tcattaaaaa
      781 caacacataa ggacttaatt atatatccat aaaatgatag caaccctaac gcaaagaact
      841 gttggaatcg taccttttcg catcgccata attatgagct ggcttttgtt tctctcgttg
      901 aagagtgtat atgcaagcga caataagcag attataacca ttagtaccca aattacacat
      961 aatgcttgtg ctggtggtaa agatggctcc atagacatca gtgtatcggg aggcttggcg
     1021 ccctattcat ttttatggtc gcagggcaat acaactgaag atttgttgtt tgtcgaaagc
     1081 ggtacctata cagtaactgt tactgatgat gccggtattg tacaaacagc agtcgctacc
     1141 ataactgaac caagccctgt tgtgataaat ttggttaacc taagtaatgt aacttgttat
     1201 agcgcaggta atggcagtat taatatttcg ccaagcggtg gtgtatctcc ttacacttat
     1261 ttatggaata atggttcaac cgtagaagac cgtgtatcat tagcacccgg gctttatacc
     1321 gttttagtta ctgatgataa tggctgtagt actaatgaaa cgtatcaaat taccggccct
     1381 tcattactta cctgcaatgt ttcaactgta aaagctataa gttgttttgg attaagtaat
     1441 ggctcggcag taataaatac caattcaggc ggcacacaac catataccta tacctggtca
     1501 agtgggcaaa ccacacaaac agccaccgga ttggcggctg gcttaacatt ggtaactgta
     1561 accgatgcca atggctgtaa ttccatttgt ggtataaata tgcaacaacc cacattatta
     1621 gtggccaact atacgcagtc aaatgttaca acaacgggcg gaagtaacgg ttcaattgat
     1681 gttacggtaa gcggaggctc acaa
//
LOCUS       DAOMAA010000498         1704 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_77005_length_1704_cov_2.973317, whole genome shotgun sequence.
ACCESSION   DAOMAA010000498 DAOMAA010000000
VERSION     DAOMAA010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77005_length_1704_cov_2.973317"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(205..840)
                     /locus_tag="PLO59_07045"
     CDS             complement(205..840)
                     /locus_tag="PLO59_07045"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQV00899.1"
                     /translation="MKIIKVIIVEDDKEIRQLAESILNNEDDIDVIKVFSTGDNFLKE
                     FERIEVDVVLMDINMPGMSGIECVAQAKPLKPHVQFLISTVFENSTYIFQALCAGATG
                     YLVKNTTGDKMAEAVREIYMGGSPMSTQIARMVVTSLGAKQTTAMHTHQLTLRENEIL
                     DLLAKGLMYKEIASKKEISTETVRKHVRNIYEKLQVTTRMEAINKVYPKSV"
     gene            complement(851..>1704)
                     /locus_tag="PLO59_07050"
     CDS             complement(851..>1704)
                     /locus_tag="PLO59_07050"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HQV00900.1"
                     /translation="KVKADTEIERQKIIRNFSVVIGMLLLFITIFIWMRYLERTKLNY
                     MLLEKNEIIQAQFESLQATQNELVLTEKQREAQSVRVRIARDIHDELGAGLTKITLLS
                     GLLRKKFETNDAETKNQLQRIIENSKSVSTSLNEIVWAVNPSNDSAESLASYLKIYAD
                     HYLEDSQINYTLHFPEDIPMLPINPELKRNVFLVLKESLNNTVKYANANKVVVSFELS
                     NNKFKLSVNDNGKGFDHEELTIKQNGASGNGLLNMKHRMEQVNCMLNITSSPGNGCMI
                     EASGTFY"
BASE COUNT          490 a          327 c          256 g          631 t
ORIGIN      
        1 aatattttca tattcttgaa tcatttaata ttttattgat aacatattta gtcctaatgt
       61 ctataaaagc tgttaaaagc ggtcctaaat taataaatct aatgtttgta gggagacctt
      121 ccagacgagt tcgacctgaa gtaattatta tttccatatt aaagaattta tttgcccaat
      181 taaatttatg caatgcacac taatttaaac tgatttagga tacactttgt tgatagcttc
      241 catgcgtgtg gtaacctgca atttttcata aatattgcgt acatgtttgc gcaccgtttc
      301 tgtactgatt tcttttttag atgcaatttc tttgtacatt aatccttttg ccaacaaatc
      361 taaaatttcg ttttcgcgca atgttaactg atgcgtatgc atagcagtag tttgttttgc
      421 acctaatgac gtaacaacca tacgtgcaat ttgtgtactc atgggcgaac cacccatata
      481 aatttcgcgt acagcttcgg ccattttatc gcctgttgta ttttttacca ggtacccggt
      541 tgcaccggca cacagcgctt gaaaaatgta agttgaattt tcgaatacag tactgattaa
      601 aaactgtaca tgcggtttta atggtttggc ttgagccaca cactcaatgc ccgacatgcc
      661 gggcatattt atatccatca acaccacatc cacttcaata cgttcgaact cctttaaaaa
      721 attatcgcct gtagaaaata ctttaatcac atctatatca tcttcattat taaggatgct
      781 ttcggccagt tgccttattt ccttatcatc ttcaactata attactttaa ttattttcat
      841 gattttactt ttaataaaat gtacccgaag cttcaatcat acagccatta cctggcgatg
      901 aagttatgtt gagcatacag tttacctgtt ccattctatg cttcatattt aataatccat
      961 tacccgaagc accgttttgt ttaatagtta attcctcgtg atcaaaccct tttccattgt
     1021 cgttaacaga tagcttaaat ttattgttac ttaactcgaa gctaaccacc accttatttg
     1081 cattagcata ttttacggta ttgtttaacg attcttttaa aactaaaaaa acatttcgtt
     1141 tcagttcggg atttatagga agcattggaa tatcttccgg aaaatgaagt gtataattta
     1201 tttgcgaatc ttctaaataa tggtctgcat atattttaag gtatgaagct aaactttcgg
     1261 ccgaatcgtt tgaaggatta actgcccaca caatttcatt aagcgatgta cttacacttt
     1321 tcgagttttc gataattcgt tgtaattgat tttttgtttc agcatcattt gtttcaaatt
     1381 tctttcgaag taagccactt agcaaggtta tttttgttag tccggctccc aactcatcat
     1441 gaatatcgcg tgcaattcgc actcttacac tttgagcttc acgctgcttt tcagttagta
     1501 ccagctcgtt ttgagttgct tgtaaagatt caaattgcgc ctgaataatt tcattttttt
     1561 caagcagcat ataatttagc ttggttcgtt caaggtatcg catccaaata aaaatggtaa
     1621 taaacagtaa taacattcct ataaccactg aaaagtttcg tattattttt tgtcgctcta
     1681 tttcagtatc ggccttgact tttt
//
LOCUS       DAOMAA010000499         1700 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_77360_length_1700_cov_3.649240, whole genome shotgun sequence.
ACCESSION   DAOMAA010000499 DAOMAA010000000
VERSION     DAOMAA010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77360_length_1700_cov_3.649240"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1155
                     /locus_tag="PLO59_07055"
     CDS             <1..1155
                     /locus_tag="PLO59_07055"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HQV00901.1"
                     /translation="NTIVREFEFNYATNKIRAATYGRGIWESSMYQQSKPAAAFIQNK
                     VLICEGQTVTYTDISTEAPTSRTWFFPGGNPATANSKQVTVYYPNAGLYDAALTVSNI
                     VGTDSITKKFTTKVETQFQAAPFVEGFESILPQGWHVDNVDNVELTWDFAGIGAYGSS
                     LKSFKAVNFNTSYSGVVDYLKTPQFDLSNLVNPLLKFDVAYAKRTPVSNDSLWVEASL
                     DCGLTWIRIYSKGGTTLRTTNSFSISQAFVPAPWQWRNESVSLGWLAQQQVQFRFVNY
                     NGSGNDIYIDNINIDVPATIANTVTTNQVWLYPNPVTDVSYLRLSDEFGNGKQVTVTI
                     YDVIGNEVATHNLSKGKEIDISKKHLPPGIYTYLISNASGNLVKGKFVVQ"
     gene            complement(1168..>1700)
                     /locus_tag="PLO59_07060"
     CDS             complement(1168..>1700)
                     /locus_tag="PLO59_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014772422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HQV00902.1"
                     /translation="IKSMRGSDPNAALYYLARMLQSGEDPKFIARRMLIFSSEDIGNA
                     NPNALLIATACFQSVQFIGYPECDLILSQAVIYLSTSVKSNASYLAIREAQQTVTETG
                     NLNVPLHLRNAPTKLMKHLDYGKNYKYPHDFENSFVREDYLPESISNKIFYNPANNAR
                     ENEIRKNLKNWWPDKY"
BASE COUNT          534 a          291 c          347 g          528 t
ORIGIN      
        1 aatacaattg ttcgcgagtt tgagtttaat tatgctacca ataaaatacg agctgccact
       61 tatggtcgtg gtatttggga aagcagcatg tatcaacaaa gcaaaccggc agcggcattt
      121 attcagaata aagtgttgat ttgtgaaggt caaacagtta cctatactga tataagcaca
      181 gaagcaccta catcacgtac gtggtttttc ccgggcggaa atccggcaac agccaattca
      241 aaacaagtaa cggtttatta ccccaatgcg ggcttatatg acgctgcgtt aactgtgagt
      301 aatatagttg gtacagatag cattacaaaa aaatttacaa ctaaggtcga aacacaattt
      361 caagcagcac cgtttgtcga aggatttgaa agtatattac cacaaggctg gcatgttgat
      421 aatgtagata atgttgagct aacctgggat tttgccggta ttggagccta tggctccagt
      481 ttaaaatcat ttaaagcagt aaattttaat acatcgtata gtggtgtagt tgattactta
      541 aaaacgcctc aatttgattt aagcaacctg gttaatcctt tattaaaatt tgacgtggct
      601 tatgcaaaac gaactccggt ttcaaacgat agtttatggg tcgaagcatc gctcgattgt
      661 ggtttaacct ggatacgaat ttacagtaaa ggaggtacta cattgcgcac tacaaattca
      721 ttctcaatca gtcaggcttt tgtaccggca ccgtggcaat ggcgtaacga aagtgtaagt
      781 ttgggatggt tggcgcaaca gcaagtacaa tttaggtttg taaattataa tggtagcggt
      841 aatgatattt atatcgacaa tattaatata gatgttccgg ccacaatagc aaatacggtt
      901 actacaaatc aggtttggtt gtaccccaat ccggttaccg atgtgtcata cctgcgttta
      961 tctgatgagt ttggtaatgg taaacaggta actgttacaa tatatgatgt tattggtaat
     1021 gaagttgcta cccataatct atcaaaagga aaggaaatag acataagtaa aaagcacttg
     1081 ccacccggta tatatacata tttgattagc aatgctagtg gtaatttggt aaagggcaag
     1141 tttgtggttc aataaactaa agtagtttta atatttatcg ggccaccagt tttttaaatt
     1201 ttttcggatt tcattttcgc gtgcattgtt tgccggatta taaaatattt tattcgagat
     1261 gctttcgggc aagtaatctt ctcttacaaa actattttca aaatcgtgtg ggtatttata
     1321 atttttacca taatctaaat gtttcataag tttagttggt gcattacgca agtgtagcgg
     1381 aacatttaag ttgccggttt ctgtaacggt ttgttgagcc tcacgaatgg ccaaatagct
     1441 ggcattgctt ttaacagatg tgcttaaata gattactgcc tgcgataaaa tcaaatcgca
     1501 ttcaggatag ccaataaact gaaccgattg aaaacaagct gttgcaataa gcaaagcatt
     1561 gggatttgca ttgccaatat cttcactgct aaaaatcaac attcttcgcg ctataaattt
     1621 gggatcttcg ccactttgta acatacgtgc caaatagtaa agtgcagcat tagggtcgct
     1681 gccacgcata cttttaataa
//
LOCUS       DAOMAA010000500         1697 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_77637_length_1697_cov_3.160171, whole genome shotgun sequence.
ACCESSION   DAOMAA010000500 DAOMAA010000000
VERSION     DAOMAA010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77637_length_1697_cov_3.160171"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /locus_tag="PLO59_07065"
     CDS             <1..928
                     /locus_tag="PLO59_07065"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV00903.1"
                     /translation="YVLNRNTYQYIDQNPALLASQPVQQYFDSVALTDLGKLESVNFI
                     YKINVSTLAALTHSRAFNDSVLLNLSLQYQTNLGLLAGNNLTATDSAILVGANATIQA
                     QIIAIGVMNDSISNTAKVYHVTQAHAAAALNQGIVTNGLYTANQKTINALYAQYAAKG
                     NNAYTIADSITILSIAQQCITYGGPAVLAARSLFLLINSNAAFDNTPCNTGSRWSMSD
                     KEPVTEKTSATLFCYPNPATDFITLYLPPTIMQYALALYDMLGNKLKYITGNKHTVLT
                     IPVADLPQGIYQIKLVSLSDGVILHQKIEVVK"
     gene            945..>1697
                     /locus_tag="PLO59_07070"
     CDS             945..>1697
                     /locus_tag="PLO59_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00904.1"
                     /translation="MKKYYLLLLLCLGYVTLHAQHTPVQWVVGIGPAGHNMFDFTTGT
                     LNIQPLTGATAHFTETSAAIGNTDSSLLYYSNGVAIYNSNHVKIQNGDSLSPSPLWPD
                     LNHGLRFYQAAVFIPYPGDSNKIYIFHYSLDSLYFPCANGTFDGGPDKLYYSVIDKTL
                     NNGHGAVIAKNNVIQNTLLGHGIFTVKHGNGRDWWVVVRDVTQPVFYRFLISPTGIAG
                     PFTQTIGPVACGGFNSMRYSALTNKMACFRVFY"
BASE COUNT          502 a          359 c          326 g          510 t
ORIGIN      
        1 ttatgtactt aaccgcaata cctatcagta tattgaccaa aaccccgccc tgcttgccag
       61 ccagcctgtg cagcagtatt ttgacagtgt tgccttaacc gatttaggta agttagaaag
      121 tgtaaatttc atatataaaa taaatgtgag cacgcttgct gcattaaccc atagccgtgc
      181 ttttaacgac tctgttttac tgaacctgag tttacaatac caaaccaact tagggttgct
      241 tgccggtaac aatttaactg caaccgacag tgctatttta gtaggggcta atgcaaccat
      301 tcaggcgcaa attattgcca ttggtgttat gaatgatagt attagtaata cagctaaagt
      361 ttaccatgta acccaggccc atgcagccgc agccttaaat caaggtattg ttaccaatgg
      421 cctttataca gccaatcaaa aaaccataaa tgcactttat gcccaatatg cagccaaggg
      481 taataatgct tataccattg ccgatagcat taccatatta agcatagcac agcaatgtat
      541 aacctatggt ggccctgctg tattggctgc gcgtagcttg tttttactta ttaactccaa
      601 tgcagctttt gataatacac cctgcaatac cggcagccga tggagcatga gtgataaaga
      661 gccggttaca gaaaaaacat cagcaaccct attttgttac cccaaccccg ctactgactt
      721 tataacattg tatttgccgc caactataat gcaatatgcc cttgctttat atgatatgtt
      781 gggcaataaa ttgaaataca ttacaggtaa caaacatact gtacttacaa tacctgtagc
      841 cgatttacca caaggtatat atcagataaa actggtttcg ttaagcgatg gtgttatttt
      901 gcatcagaaa attgaagtag ttaaataatt taatagccgc tccgatgaaa aagtactatt
      961 tattattatt gctatgcttg ggttatgtaa cgttacatgc acaacacaca cctgtgcaat
     1021 gggtagtagg cattgggcct gccggccaca atatgtttga ttttaccaca ggcaccctta
     1081 acatacaacc gcttacgggt gcaactgcac attttacaga gaccagtgca gccataggca
     1141 atacggatag cagcttgctg tattattcaa atggcgttgc catttacaac agcaatcatg
     1201 taaaaataca aaatggcgat tcgttaagcc ccagtccgtt atggccagac ttaaaccatg
     1261 gtttgcgttt ttatcaggct gcggttttta taccctaccc gggcgatagc aataaaattt
     1321 atatatttca ttactcttta gattcgcttt attttccatg cgctaatggc acctttgatg
     1381 gcggccctga caaactatat tacagtgtaa ttgataaaac cttaaataat ggccatggtg
     1441 ctgttattgc taaaaacaac gttattcaaa ataccctttt gggccatggt atttttactg
     1501 taaagcatgg caatgggcgc gattggtggg tagtagtaag ggatgttaca caacctgtgt
     1561 tttaccgttt tttaattagc cctacaggca tagccgggcc ttttacgcaa accataggcc
     1621 ctgtggcctg tggtggtttt aacagtatgc gctacagtgc tttaacaaat aaaatggcct
     1681 gctttagggt tttttac
//
LOCUS       DAOMAA010000501         1696 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_77717_length_1696_cov_3.822669, whole genome shotgun sequence.
ACCESSION   DAOMAA010000501 DAOMAA010000000
VERSION     DAOMAA010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77717_length_1696_cov_3.822669"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1696)
                     /locus_tag="PLO59_07075"
     CDS             complement(<1..>1696)
                     /locus_tag="PLO59_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00905.1"
                     /translation="GVYMTNTNASGNIADPVQLWPLHKFWGGNLGLNNGNTFKNMRYG
                     IITENIETNVTNNEFDNVAFGIKASKGVIKLQTINKNQFINTAQAIELNDNKKAVISI
                     TNNTIHTQFATSANDLIFGVRIIESAGNSSQLRIADNLIENNGLSGIEISNNYGGIIE
                     NNQIIQNSLQTETSIALSVENSDSVTISCNKVSSTDSLTIAPTQTAYKFAFAPESQVL
                     CNSSNGTAIGFQFLANCDLSDIRNNKIKAHDNGLVLGDVSLTGGVIGPQPGLNQTRGA
                     GNIFEGAYTVSTGTGAAKGFFTHAATLSVNSQAAGVQGTNTQFMVYNADSLQVPYPNL
                     SIGSNATPFTPVLKNKPSNPCPTVCAIPLKINNGETNFSNAYDYAAAVLLSNNNEDAT
                     THARKVFVLKAQNQVSTSNPSLASFTQQYKITNLGNMALVSDYYKTMKANDKDVYQKI
                     ATVLNTMQPNNIVELNWHTLELIKTENAIEQKPLSQIQYQIIKNIANQCPYIGGESVF
                     DARGIVAGIEHVVYNDATTCQTKPIVKNTHVNTLPFINAYPNPANQYFNVVYNLSDY"
BASE COUNT          462 a          289 c          329 g          616 t
ORIGIN      
        1 tataatcgct cagattatat actacattaa aatattgatt agccggattt ggatatgcat
       61 ttataaatgg caatgtatta acgtgggtat tttttactat tggttttgtt tgacaggttg
      121 ttgcatcgtt gtaaacaacg tgttcaatac cggcaacaat gccacgtgca tcaaaaacac
      181 tttcaccacc aatatatgga cattgattag ctatgttttt aattatttga tattgtattt
      241 gcgaaagcgg tttttgctcg attgcatttt cggtcttaat cagttcaagt gtgtgccagt
      301 ttaattcaac aatattgtta ggctgcattg tatttaaaac cgttgcaatt ttttgataaa
      361 catctttatc gtttgctttc atggtcttat aataatcaga cactaaagcc atgttgccca
      421 aatttgtgat tttatattgc tgtgtaaaag atgctagcga tgggttgctg gtacttactt
      481 gattttgggc ttttagaaca aacacttttc gagcatgggt ggttgcgtct tcattattat
      541 ttgaaagtaa aacagcagca gcatagtcgt aagcgttgct aaagtttgtt tctccgttat
      601 ttattttaag cggtatggcg caaaccgttg ggcatgggtt ggaaggcttg tttttaagta
      661 ccggtgtaaa tggtgtagcg ttactaccga tacttaaatt agggtagggt acttgtaacg
      721 agtcggcatt atacaccata aattgtgtat tggtgccttg tacaccggca gcttgtgagt
      781 taaccgataa tgtggcagca tgtgtaaaga aaccttttgc agcacctgta ccggtagaaa
      841 cggtgtatgc accttcaaat atattgcctg cgccacgtgt ttggtttaaa cctggttgtg
      901 ggcctataac acctcctgtt aaacttacat cgcctaaaac caagccatta tcgtgggctt
      961 ttattttgtt atttctaata tcagaaaggt cgcaattagc taaaaactga aagccaatgg
     1021 ccgttccatt gctcgagtta catagtactt gcgattcggg tgcaaatgca aatttatagg
     1081 ctgtttgcgt tggtgcaatt gtcaaactat cagtactgct tactttatta caactgatgg
     1141 taaccgaatc gctgttttca acacttagcg caattgaagt ttcggtttgc aaactgtttt
     1201 gaattatttg attgttttcg ataatgccac catagttatt acttatctca atacctgata
     1261 gaccattatt ttcaatcaag ttatcggcaa tgcgtaactg gctgctattg cctgctgact
     1321 caattattct tacaccaaaa attaaatcgt ttgccgatgt agcgaactgt gtatgtatgg
     1381 tattattggt aatggatatt actgcttttt tattgtcgtt taattcgata gcctgggctg
     1441 tattaatgaa ttgattttta tttattgttt gaagttttat tacaccttta gatgctttta
     1501 taccaaaagc aacattgtca aattcgttgt tagttacatt ggtttcaatg ttttcggtaa
     1561 taattccata gcgcatattt ttaaaagtgt ttccattatt aagccctaag ttgccacccc
     1621 aaaatttatg taagggccat aattgaacag gatctgcaat attaccactc gcatttgtgt
     1681 ttgtcatata aacacc
//
LOCUS       DAOMAA010000502         1696 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_77755_length_1696_cov_2.418647, whole genome shotgun sequence.
ACCESSION   DAOMAA010000502 DAOMAA010000000
VERSION     DAOMAA010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77755_length_1696_cov_2.418647"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1320)
                     /locus_tag="PLO59_07080"
     CDS             complement(<1..1320)
                     /locus_tag="PLO59_07080"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HQV00906.1"
                     /translation="MALNTAHTGFIKNAGQVYDYNKQVTPDVFYSLHTKAVSVFLTPK
                     GLTYSIVQREIVNDETDLKFSNKSNFTVIDKKVARVDMCLIDANIKADNIIELSYIND
                     VFVNFYGSTLNRGALNLKQCNQIIIKEVYPHIDWLLHVLEDESFKYDFIVRPGGNVSS
                     IKYKFMGAKNIIANNSADVITIETPLGKLTEGALYCFTSGNDKQIPATYTLVDDGVSL
                     NISVYDKSETLIIDPAVMVLTWATFYGGSSAIGIGATFCRAIKTDSVNNRVYIVGDCF
                     SSPFPNMNNNVAYYFSPAIGGSSAEAFITAFDLTGKQKWATYYLSTEPDVAFDVEVDN
                     NGNIYMVGSTESHNAPGGINFFTTQYQGGFIQTTNNAKPIVPGIYSYQEGFIVRFNAL
                     GHLQWATLLGGDRYDVLLSVTCDKNNNIYVCGVTDSQSKLPTNKQGNG"
BASE COUNT          528 a          311 c          268 g          589 t
ORIGIN      
        1 tccattgcct tgcttattgg tgggcaattt tgattgcgaa tcagttacac cacaaacata
       61 tatattattg tttttatcgc atgttacact aagcaacaca tcatatctgt cgccacccaa
      121 taaagtagcc cattgtaaat ggcctaatgc gttaaacctt acaataaatc cttcctgata
      181 actataaatt cctggcacaa tgggttttgc attatttgtt gtttgaataa acccaccctg
      241 atactgggtg gtaaagaaat ttataccacc cggtgcattg tgcgactctg tgctgcctac
      301 catataaata tttccattgt tatctacttc aacatcaaaa gctacatcag gctcggtact
      361 taaatagtaa gttgcccact tttgtttgcc tgttaagtca aaggctgtaa taaatgcttc
      421 agcactgcta cccccaatag ctggactaaa gtaatatgca acattattat tcatatttgg
      481 aaaagggcta ctaaaacaat cacctacaat ataaacacgg ttgttgactg aatcggtttt
      541 aatagcgcga caaaatgtag cgccaatgcc tatagccgaa cttccaccat aaaacgtagc
      601 ccaggttaat accataactg ccggatcaat tattaatgtt tctgatttat cataaactga
      661 aatgttcaaa ctcacaccgt catcaactaa tgtataggta gcaggaatct gtttgtcatt
      721 tcccgaagta aagcaataca atgcgccttc tgttaatttt cctaacggtg tttctatggt
      781 aatgacatca gccgaattat tagcaattat attttttgct cccataaact tgtattttat
      841 acttgagaca ttgccacctg ggcgcacaat aaaatcatat ttaaatgatt catcttccaa
      901 aacatgaagc aaccaatcaa tatgagggta aacttctttg ataattattt gattacactg
      961 tttgagattt aatgcgcctc tattcaatgt gctgccataa aaatttacaa aaacatcgtt
     1021 tatataacta agttcgataa tgttgtcagc ctttatgttg gcatcaatca agcacatatc
     1081 tacacgagcg acttttttat caataactgt aaaattcgat ttgttactaa attttaagtc
     1141 agtttcgtca tttactattt ctctttgaac aattgaataa gtaagtcctt tgggggttaa
     1201 aaaaactgaa actgctttgg tatgtaaact ataaaaaaca tccggtgtta cttgcttatt
     1261 ataatcatac acctgccctg catttttaat aaagcctgta tgcgcagtgt ttaatgccat
     1321 ttcggcttgt tgggcgttta cccccccccg cgggagtgcg gctattggct gctgttgtag
     1381 ctgtttgaat gcatatcagg caaaacataa tgtaggtgtt tttgtttttc atacgcttgt
     1441 agtttttggg ttaagattaa ttatattgat aaacttttat aatatgactg aaattcaggc
     1501 tacattcttt tagaaatttt ttttataaat tgaggtactt aattaattta ttttattgga
     1561 ataaaatgta ccttcaactt tttacctaat attttgattt catcatcaaa cattaattaa
     1621 attgatgttt tgaattcatt gtattcatct aattatagat caaataggag aaaatagaga
     1681 tgggtaggta aacctt
//
LOCUS       DAOMAA010000503         1695 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_77818_length_1695_cov_3.426220, whole genome shotgun sequence.
ACCESSION   DAOMAA010000503 DAOMAA010000000
VERSION     DAOMAA010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77818_length_1695_cov_3.426220"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1380
                     /locus_tag="PLO59_07085"
     CDS             <1..1380
                     /locus_tag="PLO59_07085"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HQV00907.1"
                     /translation="IHFTTAPGSNGEKHFEGVMRKMLPDANGTTLSSSWTVGQTQTLT
                     FSIPVPSYIYDLNTIGVVAFVQNASKGVEQGAYSAPIGGIPTTNDAGISAASGYSFLN
                     CASSITPSVTVKNFATAPLTSCNINYQVDGGPVTTQPWTGSLAQNATAVVSLAALNLT
                     GGVHTLKFYTSNPNNAVDYKPINNAKEATVDVVAFYYAAPLTEDFQSTQFPSNDWAIN
                     NFNNDATFTKMTGWGGFATGATASSIFLNFYNITAGKYDELYMPGLGLVGAASAQLTF
                     DVAYCQYSSENDKLEVMISNNCGTSWTTLYNKSGAALSTRPATTSNFAPTAAEWRNEV
                     VDLSNYVGQNVIIKLKATSAYGNNLWLDNINVTQVAGIADVTKGETLNVYPNPTSGLL
                     QVDANITNNGDLKVSVSDVLGNVVYSNQFTQTANKFSIDLSNQANGMYFVKFENKNGV
                     EIKKINVQK"
BASE COUNT          561 a          318 c          305 g          511 t
ORIGIN      
        1 atacatttta ctacggcccc tggttctaat ggcgaaaagc actttgaagg cgtaatgcgt
       61 aaaatgttgc ctgatgccaa tggtactacc ttatcatcaa gctggactgt tggtcaaaca
      121 caaactttaa ctttcagcat acctgtacct tcatacatct atgatttaaa tacaattggc
      181 gtagtagcat ttgttcaaaa tgcaagcaaa ggtgttgagc aaggtgcata cagcgcacca
      241 attggtggca tacctactac caatgatgca ggtatttcgg ctgcaagtgg ttattcattt
      301 ttaaattgtg cttcttctat tacaccaagc gtaacggtaa agaattttgc aactgcgcca
      361 cttacttcat gtaatatcaa ctaccaagtt gatggaggcc ctgtaactac gcaaccctgg
      421 acaggttcat tagcacaaaa tgcaactgct gttgtaagtt tagctgcttt aaacttaaca
      481 ggcggtgttc atacgcttaa gttttataca tctaatccta ataatgcagt tgattataag
      541 ccaattaata acgctaaaga agctacagtt gatgtggttg ctttttatta tgcagccccg
      601 ttaaccgaag attttcaaag cacacaattc ccatctaacg actgggctat taacaacttt
      661 aacaacgatg ctacctttac caaaatgaca ggttggggcg gttttgcgac cggagcaacg
      721 gcatcgtcaa tatttttaaa tttctataat attaccgcag gtaagtatga cgagctttat
      781 atgccaggtt taggtttagt aggagctgca tcagcccaat taacatttga tgtggcctat
      841 tgtcaataca gttctgaaaa cgataaactt gaagtgatga tttcaaacaa ctgtggtact
      901 tcatggacaa ctttatacaa taagtctgga gctgccttat caaccagacc tgcaactaca
      961 agcaactttg caccaactgc agccgaatgg cgtaacgaag tagttgattt atcaaactat
     1021 gttggacaaa atgttataat taaactgaaa gctaccagcg cttatggaaa taacctatgg
     1081 ttagataaca taaacgttac ccaggttgca ggtattgccg atgttaccaa aggcgaaaca
     1141 ttaaatgtat atcccaaccc aacatcgggt ttgttacagg ttgatgccaa cattacaaac
     1201 aatggcgatt taaaagtttc ggtttcagat gttttaggaa acgtagttta tagcaatcag
     1261 tttacgcaaa cagctaacaa atttagtatt gatctttcga accaagccaa tggtatgtat
     1321 ttcgttaaat tcgaaaacaa aaatggtgtt gagattaaaa agattaatgt acagaaataa
     1381 ttaggcagta cgtttataaa aaaagccgta gtcagtacac tgactacggc tttttttatg
     1441 actataaaat ttcattaaac acaccttaaa tttaattaac tacgttacct ttgcacctcg
     1501 caaaaactca aaatctacaa catgattaat tcattaaaaa gcgttacaat atttatttgt
     1561 ttaatagtat ttggatatag ccatgcacaa caagttggta aagtaatacc atcggtaaaa
     1621 ctgaaaacgt tagatggtaa ctttattagt acggatgact ttaacaatga tggcaaacct
     1681 attatactcg atttt
//
LOCUS       DAOMAA010000504         1695 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_77872_length_1695_cov_1.910976, whole genome shotgun sequence.
ACCESSION   DAOMAA010000504 DAOMAA010000000
VERSION     DAOMAA010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77872_length_1695_cov_1.910976"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="PLO59_07090"
     CDS             complement(<1..326)
                     /locus_tag="PLO59_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011587033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HQV00908.1"
                     /translation="MLRNERFAGVLKYFEIHFPEAETELCYNNAYELLVAVILSAQCT
                     DKRVNLTTPSLFKAYPTAVDLMDAQVDDVFALIKSISYPNNKAKHLVAMANRLVKVYN
                     GIVPNT"
     gene            complement(698..1279)
                     /locus_tag="PLO59_07095"
     CDS             complement(698..1279)
                     /locus_tag="PLO59_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQV00909.1"
                     /translation="MQHLISDNELISLYLSGNEQGLGELVHRHKTRIFTSIYVLVKDE
                     ELANDIFQDTFIKVINTLRSGNYHEEGKFLPWVLRIAHNLVIDHFRRSKRMPMLHDTD
                     EDSVFRTMKSDEYNIEEVLVTKQIHKDVRMLINKLPDEQRDVVMMRHYANLSFKEIAD
                     AMDCSVNTALGRMRYALLNMRKLMDENKISLQR"
     gene            complement(1483..>1695)
                     /locus_tag="PLO59_07100"
     CDS             complement(1483..>1695)
                     /locus_tag="PLO59_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00910.1"
                     /translation="NQKFKGQSATCFLLFFTVSLFVLIGITTPVLGSLVRYKAPLMPL
                     FFIALVWQFNYVVLKQRLNYLFSLIK"
BASE COUNT          545 a          288 c          281 g          581 t
ORIGIN      
        1 atagtattag gcacaatgcc attataaacc tttactaagc gatttgccat agcaaccaaa
       61 tgcttagcct tattattagg ataactgatg ctttttatta aagcaaaaac atcatccact
      121 tgggcgtcca tcaaatcaac agctgttgga tatgctttaa ataatgaagg cgtagttagg
      181 tttacacgct tatcggtaca ctgggccgat aaaattacag caactaaaag ttcgtatgca
      241 ttgttatagc acagttcggt ttcagcttcc ggaaaatgta tttcaaagta ttttaataca
      301 ccagcaaacc tttcgtttcg tagcataaaa ttgtgttgct aattacttgg ttaaaagtaa
      361 agcaataaaa ataaaaaagc ctgctgtttt ctacagcagg cttttttaaa gttacttttt
      421 tcgtaagtgt ttggttaatg ccgaatagca cattataatt tcaacacttt cagtactggg
      481 tgcaattgga tgcaacaatg tttgatttcc gctaggctta ctgcgacttt ttgtaccctg
      541 catgtttttt gtaacttgat gtttttgagt aattgttttg gccatacgct atttgtattt
      601 tatttgatgg ataacgccat taaattactg atattgttta tctgattaaa tagtataaaa
      661 aaaccgctcg attaaaacag agcggttttt ttaatagcta acgttgcagc gatattttat
      721 tttcatccat taattttcgc atgttaagta atgcatagcg catacgcccc aatgccgtgt
      781 ttacactgca atccatggca tcggcaattt ccttaaaact taaattagcg taatggcgca
      841 tcattaccac atcgcgttgc tcgtcaggca acttatttat aagcattctt acatctttat
      901 gtatttgctt tgtaaccaat acttcttcaa tattgtattc gtccgatttc atggttcgaa
      961 aaaccgaatc ctcatcagta tcatgaagca ttggcattcg tttgcttcgt ctaaaatgat
     1021 caattactaa attgtgggct atacgtaaaa cccatggcaa aaactttcct tcttcatgat
     1081 agttacccga gcgcaacgtg tttatcactt tgataaacgt atcctgaaaa atatcgtttg
     1141 ccaattcctc atcttttaca agaacatata ttgaggtaaa aatgcgtgtt ttatgacgat
     1201 gtaccaattc gccaagacct tgttcgtttc cggataaata aagggatatt aattcgttgt
     1261 ctgaaattaa atgttgcata gcgtaccgtt taagtaaata aataaaaact ttaaaggtag
     1321 atgtgggcta tacgagttaa ttttaggggc aatactctaa agcaatattt taaaataacg
     1381 ttgctttttg aagtgaattg cgtgtttgtg tgaattaatt ttggtaatga tagaaattat
     1441 tttgcgcatg caaaaaatta acatgagttt aaaatgaaaa acttatttaa ttaaactaaa
     1501 caaatagttc agccgctgtt tcaaaacaac ataattaaat tgccatacta acgctataaa
     1561 aaatagtggc attagtggtg ctttatatct aaccaaactg cctaatactg gtgtggttat
     1621 gcctattaaa acaaacaaac tgacagtaaa aaacaataaa aagcatgttg ccgattgacc
     1681 tttaaatttc tgatt
//
LOCUS       DAOMAA010000505         1691 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_78197_length_1691_cov_3.602689, whole genome shotgun sequence.
ACCESSION   DAOMAA010000505 DAOMAA010000000
VERSION     DAOMAA010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78197_length_1691_cov_3.602689"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..414
                     /locus_tag="PLO59_07105"
     CDS             55..414
                     /locus_tag="PLO59_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HQV00911.1"
                     /translation="MSDRGNYNDSNSEDLFSKSVRAGKRTYFFDVRATKANDYYLTIT
                     ESKKKFQGSEPGGKAVFEKHKIFLYKEDFTKFMEGLNEAVAEIKRAQPFDDSDFNNDR
                     RHDGDSSYSNVDFDDLK"
     gene            503..>1691
                     /locus_tag="PLO59_07110"
     CDS             503..>1691
                     /locus_tag="PLO59_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00912.1"
                     /translation="MAKPLVNKKKTIAAKPPAKKQKVATHPKFDTSVLIWLCVALIAT
                     AFAFWGIGNNQLTNWDDPTYITNNPLIKSLTWLQVKRIFTETYFANYQPLQILSYAIE
                     YHFFGLNAAGYHWVSLAMHVINTALVFWLGYLLCRQTLVAFIMALIFGICPMHVESVA
                     WAAERKDLLYTLFFIASLIAYVTYIQNQLKLSYLIAAFLLFTLSVFSKTMAVSLIPIL
                     FLTDWYLNRKWHWRIIAEKVPFIILAIVMGYIATTNAIETGSVDSDTFYSQIDRLFFG
                     GYNLLLYAVKLIVPFDLSGYYQYPDLPGGGIPMKFYLGFIGAVVTAIVVFYSTRYQKH
                     ILFGAGVFVSALILVVQIFPVGPTIFSERYSYIPSIGLYFVLALYINRYIQNQANSTL
                     KYLA"
BASE COUNT          513 a          302 c          306 g          570 t
ORIGIN      
        1 gtggtattaa tattttaata tatttgaccc cgaacaaaaa ctttatataa cgttatgtca
       61 gatcgcggaa attacaacga ttcgaattca gaagatttat tttcaaaatc ggtacgagca
      121 ggtaagcgta cttatttttt tgatgtgagg gctaccaagg ccaatgatta ctacttaacc
      181 attaccgaaa gcaaaaagaa atttcagggc agcgaacccg gtggcaaagc cgttttcgaa
      241 aagcataaaa tatttttgta caaagaagat tttaccaaat ttatggaagg tttaaacgaa
      301 gccgttgccg aaataaaaag agcacaacca tttgatgaca gcgattttaa taacgaccgc
      361 aggcatgatg gcgacagttc gtactcgaat gttgattttg atgatttaaa gtaaacccct
      421 attcaatttt actaaagacc tgctgccaaa agtagcaggt cttttttatt taactgcact
      481 ttactaaaag ccaatatttt ctatggcaaa accattagtt aataaaaaga aaacaatagc
      541 tgcaaaacca ccagctaaaa aacaaaaagt agcaacacac cctaagtttg atacttcagt
      601 tttgatttgg ttatgtgttg cattaatagc aacagcattt gcattttggg gaattggtaa
      661 taatcaacta accaattggg atgatccaac ctacatcacc aataatccac taattaaaag
      721 tttaacttgg cttcaggtaa aacgcatttt taccgaaacc tattttgcca attaccagcc
      781 gcttcaaatt ttatcatatg ctatcgaata tcattttttc ggtttaaatg cagccggata
      841 tcattgggta agtttagcca tgcatgtaat aaatacggca ttggtttttt ggttgggcta
      901 tttactttgc aggcaaacgt tggttgcatt tataatggca ttaatatttg gcatttgccc
      961 gatgcacgtt gaaagcgttg cctgggctgc cgagcgtaaa gatttgctgt acaccctgtt
     1021 ttttattgct tcacttattg cttatgttac atacatacaa aatcaattaa agcttagcta
     1081 tttaattgca gcgtttctct tgtttacact atcggttttt tctaaaacaa tggccgtatc
     1141 attaataccg atattgtttt taaccgattg gtatttaaac cgtaaatggc attggcggat
     1201 aatagcagaa aaagtaccct ttatcatact ggcaattgta atgggttata tagcaactac
     1261 caatgccatt gaaacaggat cggtagatag cgatactttt tattcgcaaa ttgatcgttt
     1321 gttttttggt gggtataacc tgttgctata cgcagttaaa ctcattgtgc cgtttgattt
     1381 gtcgggctac tatcaatatc ctgatttgcc tggtggcggt attcctatga aattttatct
     1441 cggttttatt ggtgctgttg ttacggccat cgttgtattt tactcgactc gttatcaaaa
     1501 gcatatttta tttggtgccg gtgtgtttgt aagtgcttta attttagtgg tacaaatttt
     1561 tccggttggg cctaccatct tttctgaacg ttatagttat atcccatcca ttgggctata
     1621 ctttgtttta gcactttata taaaccgtta catccaaaac caagcaaaca gcactttaaa
     1681 atacctggca t
//
LOCUS       DAOMAA010000506         1690 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_78319_length_1690_cov_2.427523, whole genome shotgun sequence.
ACCESSION   DAOMAA010000506 DAOMAA010000000
VERSION     DAOMAA010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78319_length_1690_cov_2.427523"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="PLO59_07115"
     CDS             complement(<1..198)
                     /locus_tag="PLO59_07115"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HQV00913.1"
                     /translation="MLSLSQLKGQSASKRDSVKADTLLALAKSQIGKNYCYAVADPEK
                     GFDCSGFVYFVFHSFKIEVPRS"
     gene            492..>1690
                     /locus_tag="PLO59_07120"
     CDS             492..>1690
                     /locus_tag="PLO59_07120"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HQV00914.1"
                     /translation="MAKLNGTQLSPARKKAFEKLNINPNHRAILCLDGGGMRGILTIQ
                     LLKRLEQIAEMPLHQLFDMVAGTSTGGIIAGLICTGHNADQIETLYESLVTKVFDKRS
                     LGNRFVNPPAFSKQQYRTLLQETVKNETLEQACHKHDIDLLITAQDMAAAEETFFACF
                     KQADNTYHGTYKNVLLRAVMEATMSAPTYFYPLERFVDGGTTTYNNPSLAAFIEAVSY
                     SCPKDKQSNYRLSELTLFSFGTGIARQFIKPDDTINPKGVDINFWLNWLMTQTPQDAS
                     AMQIDMLRSPMMNTLVDFRRFQITLDPIAIKKLPNIDALDEKKYKSKWLHDLGEDTLG
                     SIDMADVTRFDLMKVIGAQMAEYITQTGNNFRTDLVDRSRGSLVTSFGDVPRILKQMS
                     TAKWVDE"
BASE COUNT          552 a          360 c          329 g          449 t
ORIGIN      
        1 tgagcgcggt acttcaattt taaaactatg gaaaacaaaa tacacaaagc cactgcaatc
       61 aaatcccttt tcgggatcgg caacggcgta acaataattt ttacctattt gcgatttggc
      121 taaggctaat aaggtatctg ctttaacgga atctcgcttt gatgccgatt ggcccttaag
      181 ctgacttaag gacaacaaaa acaaaaaaag tatgatcaga gccgtacgca tttataatat
      241 gatcgctttt aacgctttaa gttacggctt ttatttaaca gcccttttgt ttttagaggt
      301 aaatatttat cttcaacact aagcttttaa ccaaaaaact aagtttgata ctaaccacca
      361 agacacggag acacggttgc gtcgcttaat aaaaaccgtg ccttggtgtc ttggtggtta
      421 taaaacaatc atagttaaga gtcttggtgg taaaaaaaat ggtggttaaa tgcctctaaa
      481 aaaactaatc tatggcaaaa ttaaacggca cccaactcag ccccgcacgc aaaaaggcgt
      541 tcgaaaaatt aaacatcaac cccaaccacc gagccatttt atgtttggac ggcggaggta
      601 tgcgcggcat attaaccata caattattaa aacgcttaga gcaaattgcc gaaatgccct
      661 tgcaccaatt atttgatatg gtggcgggca cttctaccgg cggaattatt gcaggattaa
      721 tttgcacagg acataacgcc gatcaaattg aaacgcttta cgaaagttta gttaccaaag
      781 tatttgataa acgcagtttg ggaaatcgct ttgtaaatcc tccggcattt agcaaacagc
      841 aataccgcac actcttacaa gaaactgtaa agaacgaaac actggagcaa gcctgccaca
      901 aacacgatat tgatctttta attaccgcgc aagatatggc cgcggccgaa gaaacatttt
      961 ttgcttgctt taaacaagcg gataatacct accacggcac ttacaaaaat gtattattgc
     1021 gcgccgtaat ggaagctacc atgagtgcac ccacgtattt ttatccttta gagcgttttg
     1081 tagatggcgg aactaccaca tacaacaatc cttcgttggc agcatttatt gaagcggtat
     1141 cttacagttg ccctaaggac aaacaaagca attaccgctt aagcgaactt accttgttta
     1201 gttttggtac aggtattgca aggcaattta taaaacccga cgataccatt aacccaaaag
     1261 gcgttgacat taatttttgg ttaaattggt tgatgacaca aacaccgcaa gatgccagcg
     1321 ccatgcaaat tgatatgtta cgatcgccca tgatgaatac cttggttgat tttagacggt
     1381 ttcaaattac gctggaccct attgccatta aaaaattacc aaacattgac gcactcgacg
     1441 aaaaaaaata caaaagcaaa tggctgcacg accttggcga agacaccctt ggcagtattg
     1501 acatggccga tgttacccgc tttgacctta tgaaagtaat tggcgcacaa atggccgaat
     1561 acattaccca aaccggcaat aattttagaa ccgaccttgt tgaccgcagc cgcggctctt
     1621 tagtaacttc gtttggcgat gtgccaagga tattaaaaca aatgagtacg gcaaaatggg
     1681 ttgatgagtt
//
LOCUS       DAOMAA010000507         1689 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_78406_length_1689_cov_2.503060, whole genome shotgun sequence.
ACCESSION   DAOMAA010000507 DAOMAA010000000
VERSION     DAOMAA010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78406_length_1689_cov_2.503060"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(613..1551)
                     /locus_tag="PLO59_07125"
     CDS             complement(613..1551)
                     /locus_tag="PLO59_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HQV00915.1"
                     /translation="MKPNNSIYVAGHKGMVGSAICRALNARGFNNIITANSNELDLRN
                     QAAVNIFFEKHQPDYVFLAAAKVGGIVANNTYRADFLYDNLMIECNVIHSAFKHNVTK
                     LLFLGSSCIYPKMAPQPLKESYLLTGLLEPTNEPYAVAKIAGIKLCESYKRQYGCNFI
                     SAMPTNLYGPNDNYSLQNSHVLPALLRKFYEAKTHNFSEVEIWGTGKPLREFMYVDDL
                     ANACLFLMQNYNDEAFVNVGTGTDISIADLALLIKEITGFTGDLKFNASKPDGTPRKL
                     MDVSKLKSLGFEPLTDLRTGISNVWSLVKNGRFFER"
BASE COUNT          565 a          292 c          298 g          534 t
ORIGIN      
        1 accgtggaca agaacgggac aaaacctgtt tagtccattt attttaaaac tgcatgacca
       61 atcagacttc aattttaatg aaatggaaga tcccggccta tcagtagctg tccattcacc
      121 tatgaatgga ttttggtttt gagcaaataa cacctgcgct gataaaaaaa gcagaaatgt
      181 taaaattaat gtgcttagtt ttctcataag taaatagttt taaagtgtcg gggtttatgt
      241 aattttttag tgttgaattt taaaaagcca acttgttata attaatggga gaacgcgcat
      301 tgctcaattt ttcaacaact gaaaaatgag cataagccaa cattaatttg aaaattaatg
      361 cggcttttaa ttgaatatga atatgcacaa gaagcccaat aaataaaggg aaaaaagggc
      421 taacaaaatc tttaaaaaag attttaaaca ttgataaaaa cagctttagg ggtaattcga
      481 aataattaac tttttgatga taaatacaca atttgaaatg ggggttcatc aaaaaatcgt
      541 aggcaattta caaactgttt acattaaaac aacaaaacgc taaatttttg caaaaaaaaa
      601 tcgtgtgaag ttctatcgtt caaaaaagcg gccattttta actaagctcc aaacattact
      661 gatacctgtt cgtaaatcag taagtggttc aaaacccaga cttttaagct tgctaacatc
      721 cattaatttt cgtggtgtgc catcaggttt acttgcattg aactttaaat cccctgtaaa
      781 tccggttatt tcttttataa gcaaggctaa gtcggctata gaaatatcag tgccggttcc
      841 tacgttaaca aacgcttcgt cattataatt ttgcattaga aacaaacatg catttgccag
      901 gtcatctaca tacataaact cacgcaacgg tttaccagtg ccccaaatct caacttcaga
      961 aaaattatgc gtttttgcct cataaaattt tcgcaaaaga gccggtaaca catgtgagtt
     1021 ttgaagactg tagttatcgt ttggcccata caaattggtt ggcattgccg aaatgaaatt
     1081 acagccatat tgccgcttat aggattcgca aagtttaatg cctgcaattt tagctactgc
     1141 atacggttcg ttagtaggct ctaataaacc ggttaggaga taggattcct ttagcggctg
     1201 tggtgccatt tttgggtaga tacaagatga gcctaaaaaa agtaatttgg taacattatg
     1261 tttaaatgcg gagtgaatta cattacactc aatcatcaga ttgtcgtata aaaaatcggc
     1321 acggtaggta ttatttgcaa ctataccacc aacctttgca gcagctaaga aaacataatc
     1381 cggttggtgt ttttcaaaaa atatgtttac cgctgcttga tttcttagat cgagttcatt
     1441 agagtttgcg gtgatgatat tgttaaatcc acgcgcgttt agtgcacggc aaatagctga
     1501 gcctaccata cccttatgac ctgctacata aatggagtta ttgggtttca agatttaaat
     1561 cttactttaa aattaacttt cgtaataatt aggcactgtg tggccaccat cttttaaata
     1621 tttatcgcgt gtaaaaagtt gcaaatcgct ttgcatcatt tcggctacaa ttgcttttaa
     1681 atcatactt
//
LOCUS       DAOMAA010000508         1688 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_78487_length_1688_cov_2.939988, whole genome shotgun sequence.
ACCESSION   DAOMAA010000508 DAOMAA010000000
VERSION     DAOMAA010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78487_length_1688_cov_2.939988"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="PLO59_07130"
     CDS             complement(<1..626)
                     /locus_tag="PLO59_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00916.1"
                     /translation="MKFKNYTNKAMLIACITASNLAQAQWTENASSVYLTSGTKNVGI
                     GTFAPAFAIDVQRTGNASAHFKSVTGTCNLIIDRGNSAATSSVSYRTAGVPTWQTGTM
                     STDNFVIRNISLGAPALAVNNSNNNVGIGTNAPAHKLTINTDNLPAVALGLDNRAAIA
                     ARNAAGVYENFLWPRENDNRTYLNYGSAGMHIRNNASTSTIFLSNDNNV"
     gene            complement(902..1570)
                     /locus_tag="PLO59_07135"
     CDS             complement(902..1570)
                     /locus_tag="PLO59_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008626101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQV00917.1"
                     /translation="MKPVKPNTMPVRVALFEDNKKYRESMSLLIEGSPGFALAGAFDN
                     ARDVLSKIEQSQPDVLLMDIEMPGISGIEALLVIKKKYPQLNVLIDTVFEDDKKIFEA
                     ICNGASGYVLKSTPPARLLDAIVETYQGGAPMSPVIAKKVLQLLQDKQLQTPQPTQLF
                     DLTQREKEILALMVNGFSYKMIAAEKGITFETVRSHIKNIYQKLHVVSMAEAVAKAVK
                     NNLA"
BASE COUNT          468 a          321 c          326 g          573 t
ORIGIN      
        1 acattattat cattagacag aaaaatagta gaagtagagg cattatttct gatatgcata
       61 cctgccgaac cataatttag ataggttctg ttatcatttt cgcgtggcca taaaaagttt
      121 tcgtaaacgc ctgctgcgtt acgcgctgca atagccgctc tgttatctaa accaagtgca
      181 actgcaggta aattatcagt attaattgtt aatttatgtg ctggcgcatt ggtgccaata
      241 cccacattgt tattagaatt atttacagct aatgccggtg cgcctaaaga tatatttcgt
      301 attacaaaat tatccgtact catagtaccg gtttgccatg tgggcacacc tgctgtacga
      361 taactaaccg atgaggttgc tgcggagtta cctctgtcta taatcaaatt gcaggtacct
      421 gttaccgatt taaaatgggc gctggcattg ccagtacgtt gcacatctat tgcaaaggcc
      481 ggtgcaaatg tgccgatgcc tacatttttt gttccacttg ttaaataaac gctgcttgca
      541 ttttcagtcc actgggcttg tgctaaatta gaggctgtaa tgcaggcaat aagcattgct
      601 ttgttggtgt aatttttaaa tttcataatg tttggatttt ttagttatta gtttttattt
      661 attaaataca taccctgtta tttatttagg gctgaataca cttggctata gttacatgca
      721 ttcaattttt aattttatat aattcgattt tttgatttgc tttttttgca aacaaactta
      781 gcatgcgggc agatggtcag tcaatcccca tttttggggg gtagcattta cccggttgtt
      841 ttaaaacaaa aaataggaag tatgtaagct atacctataa aacactaaca aaaaagtgtt
      901 ttcaggctaa gttgttttta actgctttgg ccactgcttc ggccattgat accacatgta
      961 acttttgata gatgtttttt atatggctac gcacggtttc gaaggtgatg cctttttcgg
     1021 cagcaatcat cttatagctg aaaccattta ccattaatgc cagtatttcc ttttcgcgct
     1081 gggttaggtc aaacaattgt gtgggctgtg gtgtttgtag ttgtttatct tgtaacaact
     1141 gcaaaacttt ttttgcaatt acggggctca tgggtgcgcc accctgatag gtttcaacaa
     1201 tagcatcgag caaacgtgct ggtggtgtgc tttttaaaac atagccgctg gcaccattac
     1261 aaatagcttc aaatattttt ttatcgtctt cgaaaacagt atcaataagt acattcaatt
     1321 gcgggtactt ttttttgata accagcaaag cttcaatgcc actaataccg ggcatttcaa
     1381 tatccatcag caaaacatcg ggttgcgatt gttcaatttt actcagtaca tcacgggcat
     1441 tatcaaatgc gcctgccaat gcaaaaccgg gcgaaccttc gataagtaaa ctcattgact
     1501 cgcggtattt tttattgtct tcgaacaaag ctactcttac cggcatagta ttaggtttta
     1561 cgggtttcaa atatgattat tacagagcaa actatacatc cccattttgg gggatatccc
     1621 aagttaatgt aacatgcgta ccataaccat gtacggactc aattttaagc gaagcattta
     1681 gttgcttg
//
LOCUS       DAOMAA010000509         1687 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_78597_length_1687_cov_2.414216, whole genome shotgun sequence.
ACCESSION   DAOMAA010000509 DAOMAA010000000
VERSION     DAOMAA010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78597_length_1687_cov_2.414216"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1687)
                     /locus_tag="PLO59_07140"
     CDS             complement(<1..>1687)
                     /locus_tag="PLO59_07140"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HQV00918.1"
                     /translation="GIEKITQQHLIAAQQLLPAQMLSSTLSQIQMQCNALEQLYDGVF
                     LLKELSARTLDHIVSFGELMLSQILSAKLHSIHLSHQWIDSRTLIETNDNFGYATVNF
                     ENTNAKVKAAIKAETKLYVMPGFIASTATGITTTLGRGGSDYTAAIYAAAVNASVLEI
                     WTDVSGMLTADPRIVNNAKVIQQVSYHEALELSHFGAKVIYPPTIVPVMRLGMPLIIK
                     NTFAPDDAGTIIQAHKSATNNAICGITSMDKMAMLLLEGSGMVGIPGFSKRLFEVLAF
                     AKINVILITQSSSEHSICVAIESKYASHAKKVVDEAFHYEILTKLIEPLQIEQSLSIV
                     AVVGDNMKNHSGISGRMFAALGRNGINIRAIAQGSSEKNISAVIKTHDVRKAINVLHE
                     SFFENQYKQVNLFLVGVGNVGKRLIEQIKLQQTFLMQKMRIQLRVVAVANSKQYYFSE
                     DGIDLDNCLSTLLAQPASNIKELQKLIIEKNLRNSIFVDNTANAEVGLSYAHLLEKSI
                     SVVTSNKIACASAYEHYRQLKDLANEFHASFLFETNVGAGLPVISTLNDLVQSG"
BASE COUNT          476 a          339 c          328 g          544 t
ORIGIN      
        1 gccactttgt accaagtcgt ttaaagtgct aattacaggt agcccggcac ccacattggt
       61 ttcgaataaa aaggaagcat gaaactcgtt tgccaaatct tttaactggc ggtaatgttc
      121 ataagccgag gcacatgcaa ttttattact tgtaacaaca gatatacttt tttcgagcaa
      181 atgcgcataa cttaaaccaa cttcggcatt ggcagtatta tctacaaaaa tggagttgcg
      241 taaatttttt tcaataatca gtttttgtaa ttctttaatg tttgaagcag gttgggctaa
      301 caatgtactt agacagttat ctaaatcaat accatcttca ctaaagtaat attgtttgct
      361 attggcaaca gcaaccacac gcagttgtat gcgcattttt tgcatcagaa aagtttgttg
      421 cagttttatt tgttcgatta ggcgtttgcc cacattacct acgcctacca aaaataaatt
      481 tacctgtttg tattgatttt cgaaaaacga ttcgtgcaac acattaatag cttttcgaac
      541 atcatgtgtt ttaattacag ccgaaatatt tttttcggac gagccttgtg ctatagcgcg
      601 tatattaata ccgttacggc ctaatgcagc aaacatgcgc ccgcttatac cactgtggtt
      661 tttcatgtta tcgccaacta cagctactat cgaaagcgat tgttcaattt gtaatggttc
      721 aataagtttg gtaagtatct cgtaatggaa tgcttcatca accacttttt tagcatgcga
      781 tgcatatttg ctttctattg ctacgcaaat agaatgttcg gatgagcttt gcgtaataag
      841 tatcacatta atttttgcaa aagctaacac ttcgaacaag cgtttcgaaa aacccggaat
      901 acccaccatg ccactacctt cgagtaatag catagccatt ttatccatac tggttatacc
      961 acaaattgca ttgttggtgg ctgatttatg ggcctgaatg atggtgcctg catcatcagg
     1021 tgcaaacgtg tttttaatta ttaacggcat gcctaaacgc attaccggca ctattgtagg
     1081 cgggtaaatt actttggcgc caaagtgcga aagctctaaa gcttcgtgat aggaaacttg
     1141 ctgtattact ttagcgttgt ttacaatgcg cgggtcggca gtaagcatac cgcttacatc
     1201 ggtccaaatt tctagtacgg atgcattaac tgcagcagca taaatggcag cggtataatc
     1261 gctgccgcct cggcccaacg ttgttgttat gcctgttgct gttgatgcaa taaaaccggg
     1321 cattacatat aacttggtct cagctttaat agccgctttt acctttgcat ttgtgttctc
     1381 aaaatttaca gttgcatacc caaaattatc gttggtttcg attagtgtac gcgagtcaat
     1441 ccattggtgt gaaaggtgta tgctatgcaa tttagccgat aatatttgtg atagcataag
     1501 ctcgccaaaa ctcacaatat gatctaaggt gcgggccgat agctccttca ataaaaacac
     1561 accatcataa agctgctcca gtgcattgca ttgcatttgt atttgactta aagtgctgct
     1621 aagcatttga gccggtagca actgttgtgc agcaattaag tgttgctgtg taattttttc
     1681 tatgccg
//
LOCUS       DAOMAA010000510         1685 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_78749_length_1685_cov_3.146012, whole genome shotgun sequence.
ACCESSION   DAOMAA010000510 DAOMAA010000000
VERSION     DAOMAA010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78749_length_1685_cov_3.146012"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..486)
                     /locus_tag="PLO59_07145"
     CDS             complement(73..486)
                     /locus_tag="PLO59_07145"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013455306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HQV00919.1"
                     /translation="MASVFSKIIEGSLPGYIIAQNNDAIAFLDIQPLAIGHVLIVPKK
                     EVDYIFDLDDETYQSIHALAKKVAHAQRKVIPCIRIGVAVIGLEVPHAHIHLIPLNKI
                     SDINFSLPKLNVSAEQLKSTCELIKAAYQNLYGSS"
     gene            complement(489..959)
                     /gene="greA"
                     /locus_tag="PLO59_07150"
     CDS             complement(489..959)
                     /gene="greA"
                     /locus_tag="PLO59_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013306175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HQV00920.1"
                     /translation="MSISYLTKEGFAKLKDELAILKNVERPKASKAIAEARDKGDLSE
                     NAEYDAAKDAQGLLELRISKLEDVVANARIIDESKIDTSIVAVMSKVTILNHNSKKEV
                     VYTLVAESEADLKTGKISVSSPIGKGLLGKRKGDLAKVQVPAGTLSLEIIEISR"
     gene            complement(1038..>1685)
                     /locus_tag="PLO59_07155"
     CDS             complement(1038..>1685)
                     /locus_tag="PLO59_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3308 domain-containing protein"
                     /protein_id="HQV00921.1"
                     /translation="SFINIGLSYAKEFSNRIYGGITVRLVSEAIADVKASGIAFDAGV
                     QYVTGFNETKDNFKFGLSLKNVSAPMRFSGDGLSYRGTAISGDYQMSVENKSASFELP
                     SLINIGVTYDAKLAKDHRLTVAGSFVANSFTNDQFILGLEYGFKSYLMLRGGFVYEKD
                     IFDEELRTTALTGPCAGVTLEVPLGKGGKTFGVDYSYRATNPFNGTHSFGARLNL"
BASE COUNT          534 a          329 c          267 g          555 t
ORIGIN      
        1 caaaataaat aaggctaatc aattacaaat tgccattaaa actgaagaag agtttattag
       61 actacttaat gattaagagc tgccatataa gttttggtat gcggctttta ttaattcaca
      121 agtactcttt aattgctcag ccgatacatt aagttttggt aatgaaaaat taatgtcact
      181 tattttgttt agtggtatca gatgtatatg tgcatgtgga acttctaaac caataacagc
      241 tacaccaata cggatacatg gaataacttt tctctgtgca tgtgctactt tcttagccaa
      301 tgcatgtatt gattgatagg tttcatcatc cagatcaaaa atataatcta cttctttttt
      361 aggcacaatt aaaacatggc caattgccaa tggttgaata tccagaaatg caattgcatc
      421 attgttctga gcaataatat aaccgggcaa actgccttca ataattttag aaaatacgga
      481 tgccatttct aacgtgatat ttcgataatt tcaagtgata atgtaccggc tggtacctga
      541 acctttgcca aatcgccttt acgtttgcct aataaacctt ttcctattgg gctactcacc
      601 gaaatctttc cggtcttaag gtcggcttcg ctttcggcaa caagtgtata aaccacttcc
      661 tttttagagt tatggttaag tatagtaact tttgacatta cagcaacaat tgatgtatca
      721 atttttgatt catcaataat acgtgcattg gcaaccacat cttctaattt tgaaatacga
      781 agttctaata atccctgtgc atcttttgct gcatcatatt ctgcgttttc gctcaggtcg
      841 cctttatcgc gggcttcggc aatggccttt gaagctttag ggcgttccac attttttaaa
      901 attgccaatt cgtcttttag cttggcaaaa ccttctttag ttaagtatga aatagacatt
      961 ttttaatgaa tataaaaacg aaagaagacc ctcaaatgag agtcttcttt taaaatttag
     1021 tttgcaaaga tagtttttta caaattcaaa cgcgcaccaa aactgtgtgt tccgttaaat
     1081 ggattggttg cacgatacga gtaatcaaca ccaaatgttt taccaccttt gccaagaggt
     1141 acttctaaag ttactccggc acagggtcct gttaaagcag ttgtacgtaa ctcctcatca
     1201 aaaatatctt tttcataaac aaagccacct cttaacatca ggtatgactt aaacccatat
     1261 tctaagccca gtataaattg gtcgttagta aatgagtttg ccacaaatga accagctaca
     1321 gtaagtctat gatcttttgc aagtttcgca tcgtaagtaa caccaatatt aattaatgat
     1381 ggtaattcaa aactggcaga tttattttct acactcattt gataatcacc actaattgct
     1441 gttcctctgt atgacaaacc atcgccacta aaacgcatag gcgctgaaac gttttttaaa
     1501 gataagccaa atttaaaatt atcctttgtt tcattaaatc cggttacata ttgtacgcct
     1561 gcatcaaaag caatgcccga tgcttttaca tcggcaatag cctctgatac caatctaaca
     1621 gtaataccgc cataaatgcg gttactaaac tctttggcgt aggacaaacc aatatttata
     1681 aatga
//
LOCUS       DAOMAA010000511         1683 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_78943_length_1683_cov_3.078010, whole genome shotgun sequence.
ACCESSION   DAOMAA010000511 DAOMAA010000000
VERSION     DAOMAA010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78943_length_1683_cov_3.078010"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1683)
                     /gene="ccoO"
                     /locus_tag="PLO59_07160"
     CDS             complement(<1..>1683)
                     /gene="ccoO"
                     /locus_tag="PLO59_07160"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HQV00922.1"
                     /translation="WGWQLIIVSAAITLPLGFNSSKEYAELEWPIDIAITLVWVVFGI
                     NLFGTILKRRERHLYVAIWFYIATVVTVAVLHIVNSMALPVSMFKSYSVYAGVQDALV
                     QWWYGHNAVAFFLTTPFLGLMYYFVPKAANRPVYSYRLSIIHFWALIFLYIWAGPHHL
                     LYTALPDWAQSLGVVFSVMLIAPSWGGMINGLLTLRGAWDRVRDNVELKFFVVAITAY
                     GMSTFEGPLLSLKSVNAIAHYTDWIVGHVHIGGMGWNGFLIFGTLYWLIPKIYGTQLW
                     SKKLAGFHFWIGTMGIMFYAIPLYFAGFTQHFMWKEFTPDGVLRYPNFLETVVQILPM
                     YKLRALGGSLYLIGAITMVYNLVKTAYAGKLIANEAAEAAALEKNYVEHPGSHWHRWI
                     ERKPLQMLVWSLIVILIGGAIEIIPTFMVKSNVPTIASVKPYTPLELLGRDIYVREGC
                     NTCHSQMVRPFRFETERYGEYSKPGEFVYDHPFLWGSKRTGPDLQREGGRYPDSWHYN
                     HMEDPRIMSPGSMMPPYAHLIEDDLDTNLLGAKIRAMQKLGVPYPQNYDGVAG"
BASE COUNT          529 a          373 c          332 g          449 t
ORIGIN      
        1 ccggcaactc cgtcataatt ttgaggatag ggaacaccaa gtttttgcat ggctcttatt
       61 ttagcgccaa gtaaattggt atccaaatca tcttcgataa gatgtgcata cggtggcatc
      121 atactaccgg gcgacatgat gcgggggtct tccatgtggt tgtaatgcca ggaatcggga
      181 taacggccac cttcgcgttg caaatcaggg ccggttcgtt tactgcccca taaaaaagga
      241 tggtcgtaaa caaattcacc tggtttcgaa tattcgccat accgttcggt ttcgaaacgg
      301 aacggacgta ccatttgcga atgacaggtg ttgcaacctt cgcgcacgta aatgtcgcgc
      361 cccagcaatt ctaatggtgt gtaaggtttt acacttgcta tggtaggcac atttgatttt
      421 accataaatg ttggtatgat ttcaatggca ccgccaataa gtattactat taaactccaa
      481 accagcattt gtaagggttt gcgctcaatc caacgatgcc agtgactacc tggatgttcg
      541 acataatttt tttcaagagc agcagcttct gcagcttcat tggctatgag tttaccggca
      601 taggcagttt taaccaggtt ataaaccata gttattgctc caatcagata aagcgatcca
      661 cctaaagcac gtaacttata catgggtaat atttgtacaa cagtttctaa aaagttgggg
      721 taacgcaata caccatcggg tgtaaactct ttccacataa aatgttgtgt aaaccctgca
      781 aagtatagtg gtatcgcata aaacattatg cccatggtgc ctatccagaa atgaaaaccg
      841 gcaagttttt tcgaccacaa ttgtgtgccg taaattttag gtataagcca atacaaggtt
      901 ccgaaaatca gaaaaccatt ccagcccata ccaccaatat gcacatggcc tacaatccaa
      961 tcggtataat gtgcaatagc atttactgat tttaatgaaa gcagcggacc ttcgaatgtt
     1021 gacatcccat aagcggtaat tgcaacaaca aaaaatttaa gctctacgtt atcgcgcact
     1081 ctatcccaag caccacgcag ggtaagcaat ccgtttatca taccacccca cgatggagca
     1141 atcaacatca cagagaaaac tacacccagc gattgtgccc aatcgggtaa tgcagtgtat
     1201 aataagtgat gtggccctgc ccagatgtat aaaaatatta atgcccaaaa atgtatgatg
     1261 gataagcggt aagaataaac cggacggttt gcggctttag gaacaaagta atacatcaac
     1321 cctaaaaaag gtgttgtaag aaaaaatgca acagcattat ggccatacca ccattgcacc
     1381 aatgcatctt gaacacctgc atatactgag tagcttttaa acatgcttac aggaagcgcc
     1441 attgagttaa caatgtgtaa tacggctact gtaacaactg ttgctatata aaaccatatt
     1501 gcaacgtata ggtgccgctc gcgtcttttt aaaatggtgc caaataaatt gataccaaac
     1561 acaacccaaa ccaatgtgat tgcaatatct attggccact ctaactctgc atattcttta
     1621 ctgctgttaa aacccagtgg taatgttatg gcagcactaa ctatgatgag ttgccaaccc
     1681 cat
//
LOCUS       DAOMAA010000512         1680 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_79232_length_1680_cov_2.486154, whole genome shotgun sequence.
ACCESSION   DAOMAA010000512 DAOMAA010000000
VERSION     DAOMAA010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79232_length_1680_cov_2.486154"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="PLO59_07165"
     CDS             <1..250
                     /locus_tag="PLO59_07165"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HQV00923.1"
                     /translation="GYVGISYLEKTVKAISISYDKGVSYIMPSYVNAKNKSYPVTRPL
                     FYFYEKKNEAKVQKFVNYLLSDAGQAIVKEVGYIDVKK"
     gene            339..1544
                     /gene="pstC"
                     /locus_tag="PLO59_07170"
     CDS             339..1544
                     /gene="pstC"
                     /locus_tag="PLO59_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HQV00924.1"
                     /translation="MIKHFLKDFKERFIEGVLFLSSTTTSILVVLIVVYLFKEGVGIF
                     NRTPVAEGSELLVSAANKVNKLTADEVRKIFEQEFTKWNQVGGSDAEIELFRLDDITN
                     YYTDEQLGENFENMPHCIDTILMQHPNMIAFVSKQYVLPNGRACKVSIDKIAVKQVML
                     GNRWYPTASPVVELGIWPLLLGTIWVSIGAILLALPFGLAAAIYMAEIADSRIRKILK
                     PLIELLAGIPSVVYGFFGLVVMVPIIQQTFNLDVGQSALAGSIILGIMALPTIITISE
                     DAIRNTPRAMREASLALGANHWQTIVRVVLPYSVSGITAAVILGIGRAIGETMAVLMV
                     TGNAAVVPTSFLQPVRTIPATIAAELGEAPFGGIHFQALFFLGCMLFLLTFIINVIVE
                     MLAPKKLKR"
BASE COUNT          515 a          275 c          354 g          536 t
ORIGIN      
        1 tggatatgtg ggcatatcgt acttggaaaa aacggttaag gctatttcaa ttagttacga
       61 taagggtgtt agttacataa tgccatctta tgtaaacgct aaaaataaaa gttatccggt
      121 aacacgtcca ttgttttact tttatgagaa gaaaaacgaa gccaaggttc aaaaatttgt
      181 caactacttg ctatcagatg caggtcaagc aattgtgaaa gaagttggat atattgacgt
      241 taaaaaatga aatgcaaatt gtacagttgc atcataaccc gatacctggt ttgtttttat
      301 tgatttgttg ccttacagta attgattaaa aaagggaaat gattaaacat tttttaaaag
      361 actttaagga acgttttatt gagggtgtct tgtttttatc aagtacaacc accagtattt
      421 tagtagtgct tattgtagtt tatttgttta aagaaggtgt gggtattttt aaccgaaccc
      481 ctgtagccga aggttctgag cttttggtga gtgcagccaa taaagtaaac aaactgacgg
      541 ccgatgaagt aagaaaaatt tttgaacaag aatttacaaa atggaatcag gttggtggtt
      601 cagatgccga aattgaatta ttccgtttag acgacataac caactattac acagatgaac
      661 aattgggcga aaattttgaa aacatgcctc attgtattga tactatttta atgcaacatc
      721 ccaatatgat tgcatttgta agtaagcaat atgtgttgcc taacggacgg gcctgcaaag
      781 tatctattga taaaattgca gttaaacagg taatgcttgg caaccgatgg tatcctacgg
      841 caagcccagt agttgaactt ggtatatggc cattgttatt aggcaccatt tgggttagta
      901 ttggtgctat attgctggca cttccctttg gcctggcagc agctatttat atggccgaga
      961 ttgcagatag ccgtatccgt aaaattttaa aaccactaat cgaattgtta gccggcatac
     1021 catctgttgt gtatggtttt tttggcttag tagttatggt acccattata caacaaacat
     1081 ttaatttaga cgtaggccaa tcggcattag cgggtagcat tatattaggt ataatggcat
     1141 tgcctaccat tattaccata agcgaagatg caatacgtaa taccccacgc gccatgcgtg
     1201 aagcaagttt ggcattaggc gcaaaccatt ggcaaaccat tgtaagggtg gtgttgcctt
     1261 attccgtttc gggcataaca gcagcagtaa tattgggtat cggacgtgcc attggcgaaa
     1321 ccatggctgt gcttatggta accggtaatg cagccgtggt gcccacatca tttttacagc
     1381 cggtacgtac tataccggct accattgctg ccgaattagg cgaagcaccg tttggcggta
     1441 ttcattttca agcattattc tttttagggt gcatgttgtt tttactaacg tttataataa
     1501 atgtaattgt tgaaatgctg gcacccaaaa aactcaaacg ataacacaaa ggttgtatgg
     1561 attttcagac aagaaagaat atgaaagaaa aaatggcctt tggtgcttta agacttttta
     1621 gttttagcat tatagctatt ttatttttta tacttggata catagttatt aatggcattt
//
LOCUS       DAOMAA010000513         1679 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_79316_length_1679_cov_2.397167, whole genome shotgun sequence.
ACCESSION   DAOMAA010000513 DAOMAA010000000
VERSION     DAOMAA010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79316_length_1679_cov_2.397167"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="PLO59_07175"
     CDS             <1..318
                     /locus_tag="PLO59_07175"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQV00925.1"
                     /translation="NITYVSYLNTATLKYWLQHIPVIICRSGYSTLMDLSALNLKAIV
                     IPTPGQYEQMYLAQYHHQAGNVWAVNQNRFNLNEAINGYHKTKGFQVSLPLDYMQFID
                     EII"
     gene            complement(315..785)
                     /locus_tag="PLO59_07180"
     CDS             complement(315..785)
                     /locus_tag="PLO59_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00926.1"
                     /translation="MKKLLFVLLIAISVYSCRKTSNISNIPKITFTSLTGIQYSSGKD
                     SIAVLKFEYEDGDGDLGSLSSDSTGYRNLFVKYMIMQNGIYITPTLPVGFDAQIPNLT
                     PKAKNKSISGDITYNLILPPAAVSDTIKFEVYIIDRANHKSNVFETSPLIITTP"
     gene            914..1426
                     /locus_tag="PLO59_07185"
     CDS             914..1426
                     /locus_tag="PLO59_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HQV00927.1"
                     /translation="MALIKTVLDKTPVMGQHCYLAENATLIGDVTMGNNCSVWFTAVV
                     RGDVNSIVMGNNVNVQDGAIIHCTYQQAATVIGNNVSIGHRAIVHGCTIKDNALIGMG
                     AIVMDHAIVEENALVAAGAVVLENTVVESGCIYAGVPAKKVKQLSSQHFKDINMRIAN
                     NYALYASWFQ"
     gene            1437..>1679
                     /locus_tag="PLO59_07190"
     CDS             1437..>1679
                     /locus_tag="PLO59_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00928.1"
                     /translation="MKTLATYLLILLQACSYAQGQTAMRDMAYKQLIALSQGTLLVQL
                     PEYKNTIDAMRKADAKKADQFAAELNTMHQQIISAFN"
BASE COUNT          542 a          301 c          337 g          499 t
ORIGIN      
        1 aacataacat acgtaagtta tttaaacacg gctacattaa aatactggct gcaacatata
       61 ccggttatta tttgccgtag cggatactca actttaatgg atttatctgc tttaaaccta
      121 aaagcaatcg taatacccac accggggcaa tatgagcaaa tgtacctggc acaatatcat
      181 caccaggcag gtaatgtgtg ggcagtaaat caaaatcggt ttaatttaaa cgaagccatt
      241 aatgggtacc ataaaaccaa agggtttcaa gtatcattgc cgcttgatta catgcaattt
      301 attgacgaaa taatttaagg ggtagttata attaaagggc tggtttcgaa aacattgctt
      361 ttatgattgg ccctgtcaat aatatatact tcaaacttta tagtgtcgct aactgcagca
      421 ggaggcaata tcaggttgta ggtaatatcg ccacttatgg atttgttttt tgctttgggg
      481 gtcagattgg gtatttgtgc atcaaaacca acgggtaatg ttggtgtaat gtaaatgcca
      541 ttttgcataa tcatgtactt tacaaataaa ttacgatatc cggtcgagtc gctgctaagc
      601 gaacccaaat cgccatcgcc atcttcgtat tcaaatttta aaacggctat cgagtctttg
      661 ccactgctat attgaatacc ggttagtgat gtaaatgtta ttttgggtat gttggatata
      721 ttactcgttt tacggcaact gtacacacta atggcaatta acaatacaaa gagtagtttt
      781 ttcatagttc aaaaataatc agttttgtat taatgattat aataaaatat atttttgctt
      841 ttacatgaca acaaagcaac ccgcatcttg ccgtttgctt tgttaagcag ataagtaatt
      901 tagcaagcaa caaatggcat taattaaaac agttttagat aaaacgcctg ttatgggcca
      961 acattgttac ctggccgaaa acgccacctt aattggcgat gtaactatgg gcaacaactg
     1021 cagtgtatgg tttacagccg tagtgcgtgg tgatgtaaac agcatcgtaa tgggcaataa
     1081 tgtaaatgtg caggatggag ccattatcca ttgcacctat cagcaagccg ccacggttat
     1141 tggtaacaat gtttcaatag ggcaccgagc tatagtgcat ggctgcacta ttaaagataa
     1201 tgcacttatt ggcatggggg caattgttat ggaccatgcc attgtagaag aaaatgcatt
     1261 ggtagctgca ggtgctgtag tgctcgaaaa caccgttgtt gaaagtggtt gtatttatgc
     1321 cggtgtgcct gctaaaaaag taaaacaatt aagttcgcaa cactttaaag atatcaatat
     1381 gcgcatagcc aataattatg cattgtatgc aagttggttc caataattta aaaatcatga
     1441 aaacattagc aacctatctt ttaattttac tccaagcatg tagttatgct cagggccaaa
     1501 ccgccatgcg cgatatggca tacaaacaat tgattgcatt gagccagggt acattgctgg
     1561 tacaattgcc cgagtataaa aacacgattg atgctatgcg caaagccgat gcaaaaaagg
     1621 ccgatcagtt tgcagccgaa ttaaacacaa tgcatcagca aataataagc gcttttaat
//
LOCUS       DAOMAA010000514         1676 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_79526_length_1676_cov_3.928439, whole genome shotgun sequence.
ACCESSION   DAOMAA010000514 DAOMAA010000000
VERSION     DAOMAA010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79526_length_1676_cov_3.928439"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1676
                     /locus_tag="PLO59_07195"
     CDS             <1..>1676
                     /locus_tag="PLO59_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00929.1"
                     /translation="IPDISYAAGYSVTQPFGAGGYSSIDTSTGLSQYFAPSQGFYVVA
                     VEIREYRNGVLIGISRLDYQLIFIACPINPAPIALSGGLTVFSVQEGQQICFPIIFRD
                     PNGDSLSTTHQGPIFNSAFTNPPATLIDAVGDSVVSAQFCWPTSCLQGSNVPYQFTVT
                     AADNGCPAKTTSQVYTINVIPFMGTTVLNGPDTLCANTLTGISFSTTGAANSTYNWTI
                     TNGVQVTGANTNFISVDFGPTGPYIVSVREVNALGCEGSNQATKTITIIPAPVADAGL
                     PKSFCSGGSAAIGSAAIAGNIYTWSPATGLNSTTSSNPTVTLTNGTGGPIATKYIVSV
                     SANGCVSTDTVEITVNPLPISNAGNNQFVCSGNSITIGTLFTGGYTYNWTPTNGLSSS
                     SVSNPNVSLINAGSVADTTIYTVLTTNAFGCTSSDAVLVITNPLPVVAAAATDSSICP
                     GTPITLSGNGANTYSWALLTNPGTSIGSNNTLNIAPLTTTSYILTGTSSVNCINKDTI
                     AINVFAAPVVVANVSDDTVCVATTATLSATGGVSYNWATIAAPTTSIGIGA"
BASE COUNT          461 a          385 c          355 g          475 t
ORIGIN      
        1 attcccgata tttcttatgc tgcagggtat agtgttacac agccttttgg tgccggtgga
       61 tatagtagta ttgataccag tactggttta agccaatact ttgcgcccag tcagggtttt
      121 tatgttgttg ctgtcgaaat tcgcgaatac cgtaatggtg ttctcatcgg tatttcgcgt
      181 ttggattatc aactgatatt tattgcctgt cctataaatc cggcacccat agcgttaagt
      241 ggcggtttaa ctgtattctc agttcaagaa ggacaacaaa tttgtttccc cattattttt
      301 cgtgatccta atggcgatag cttatccaca acccaccaag gtcctatatt caacagtgca
      361 tttaccaatc ctccggctac cttaattgat gccgttggcg atagtgtggt ttcagcgcag
      421 ttttgctggc ctacatcgtg tttgcaagga agtaatgtac cttaccagtt tacggtaacg
      481 gctgctgata atggttgccc ggcaaaaacc accagccagg tttataccat aaacgttatt
      541 ccatttatgg gcacaacggt attaaacggc cccgatacat tatgtgcaaa cacattaaca
      601 ggcattagtt tttctactac aggtgcagcc aactcaacct ataactggac tataaccaat
      661 ggcgtgcaag taaccggggc caacaccaat tttatctctg ttgattttgg acctaccgga
      721 ccttatatcg tttcggttcg cgaggttaat gccttgggtt gcgaaggcag taatcaggca
      781 acaaaaacca ttactattat accagctcct gttgcagatg caggtttacc caagtcgttt
      841 tgctctggtg gaagcgctgc aattggctcg gctgcaattg caggtaatat ttacacctgg
      901 agtccggcca caggcttaaa cagtacaaca agcagtaacc ctaccgttac actaacaaat
      961 ggtaccggtg gtccaattgc aacaaagtat atagtatccg ttagtgctaa tggttgtgta
     1021 agtaccgata ccgtagaaat aaccgtaaac cctttaccaa taagtaatgc cggcaataat
     1081 cagtttgtat gtagcggcaa cagcattacc attggtaccc tttttaccgg tggttatacc
     1141 tataactgga cacccacaaa cggattaagc agcagttcgg tttcgaaccc caatgtgagc
     1201 ttaattaatg caggcagtgt tgccgacaca accatttata ccgtgcttac taccaatgca
     1261 tttggttgca catcgagcga tgcggtttta gtaataacca atccattgcc agtggtagca
     1321 gcagcagcaa ccgatagttc tatttgtccg ggcacaccca tcactttaag cggtaatggt
     1381 gccaatacgt attcatgggc actgctaacc aatcccggaa cgagtattgg aagcaacaat
     1441 acattaaaca tagcaccttt aactactacc agttatattt taaccggtac cagttctgtt
     1501 aactgtatta ataaagatac aattgccata aatgtatttg ccgcaccggt agttgttgca
     1561 aatgtgagcg atgataccgt ttgcgtggcc acaacagcta ctttatcggc aacgggaggc
     1621 gtaagctaca attgggcaac cattgcagcg ccaacaacat ccataggcat aggggc
//
LOCUS       DAOMAA010000515         1675 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_79646_length_1675_cov_2.958025, whole genome shotgun sequence.
ACCESSION   DAOMAA010000515 DAOMAA010000000
VERSION     DAOMAA010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79646_length_1675_cov_2.958025"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            272..358
                     /locus_tag="PLO59_07200"
     tRNA            272..358
                     /locus_tag="PLO59_07200"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:306..308,aa:Ser,seq:gct)
     gene            467..955
                     /locus_tag="PLO59_07205"
     CDS             467..955
                     /locus_tag="PLO59_07205"
                     /EC_number="5.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HQV00930.1"
                     /translation="MVKGFKFLFLISIVLLVYSCQSTIYLVRHAEKQSEADAALLSEI
                     GLQRAQRLKNWMFDKHIDSVFASTYVRTQQTAQPTATYYRKSLTIYHPDTSALLVHNL
                     SRLSKTCLIVGHTTNIPVMIRELTQHHISIGLNEFSNIYILKTKRCLFKKRTTLQVAH
                     FE"
     gene            complement(956..>1675)
                     /locus_tag="PLO59_07210"
     CDS             complement(956..>1675)
                     /locus_tag="PLO59_07210"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HQV00931.1"
                     /translation="PTKNKTIIYGGDTINVKDSKGLKQGLWMKFYKTDTIFSKGQFKN
                     DKPYGTFYAYYANGKINSTRVYDAAGRKSTFKSYYESGKLKLIGYYNQQKKDSVWKYY
                     GETDSLVATEFYKQGVEHGEWVIYYLSGRKAEIITYQNGKKNGVSKKFFANGTAKSEC
                     SYKDDKLNGYYKSYYSNGKLYLEGPYKNNLRDGMWRLHHTDGSIKASTTFKNGKGDYA
                     VLEEVVDETQPYTPSTNDNQE"
BASE COUNT          506 a          321 c          265 g          583 t
ORIGIN      
        1 actgacacaa aaaaataata atatgaataa gtataaatag ttcattactt attagtggtt
       61 atgcttttac aaatgaaatt atttacttga aacgaatagt tgcctaattt tataaatgct
      121 atagtttgtt catatcggaa acacttttta cattattaaa acaccaaact atgcaaaaat
      181 gtttgctaaa cacccataaa caggaggtaa acaaagtatt gaaaatattt ttgcataaag
      241 tgaagctatt tatacttttg ccgcccacaa cggagaggtg cctgagaggc cgaaaggaac
      301 agtttgctaa actgtcgtac gggtaactgt accgcgggtt cgaatcccgc tctctccgct
      361 tgtttttaag cagttaggca accaagctac ttttcttcaa cgttccacag cttcgtacta
      421 aactttataa gttttacctg atttatttat aagcaagttt atgtatatgg ttaagggttt
      481 taaatttttg tttttaataa gcattgtact tttagtttat agttgtcagt caacaattta
      541 tttggtgcgt catgccgaaa agcaaagcga agcagatgcg gcattactat cagaaattgg
      601 tttgcaacgt gcccaacgct taaaaaactg gatgtttgat aagcacatag attctgtttt
      661 tgcatcaacc tatgtacgaa cacaacaaac ggcacaacct actgcaacat attatcgaaa
      721 gtcattaact atttatcatc ccgatacttc ggcactattg gttcataacc taagtcggct
      781 ttctaaaact tgccttatag ttgggcatac aaccaatatt ccggttatga ttcgcgaact
      841 aactcaacat catataagta ttggcttaaa cgagtttagt aatatttata tattgaaaac
      901 caaacgctgc ttatttaaaa aacgcactac acttcaggta gctcatttcg agtaattatt
      961 cttgattgtc gtttgttgac ggtgtgtatg gctgtgtttc gtcaacaact tcttctaaaa
     1021 cagcataatc acctttacca tttttaaatg ttgtacttgc ctttatgctt ccatcggtat
     1081 ggtgcaaacg ccacatgcca tcgcgtaaat tgtttttata cggcccttct aaataaagtt
     1141 ttccattgct gtaatagctc ttatagtaac cgtttaattt atcatccttg taactgcact
     1201 cagatttggc tgtaccattg gcaaaaaact ttttgcttac accgttcttt ttcccgtttt
     1261 gataggtaat tatttctgct tttcggcctg acagataata aattacccac tcgccatgtt
     1321 caacaccttg tttgtaaaat tcagtagcca ctaacgaatc ggtttcgcca taatatttcc
     1381 aaacgctatc tttcttttgt tggttataat aaccaatcaa tttaagtttt cccgactcat
     1441 aataactctt aaaagttgat ttacgaccgg ctgcatcata cacacgtgtt gaatttattt
     1501 ttccgttagc gtagtaagca taaaaagtac catacggctt atcgttttta aactgcccct
     1561 tcgaaaaaat ggtatcggtt ttataaaatt tcatccaaag gccttgtttt aaacctttgc
     1621 tatctttcac attaatggta tcaccgccat aaattatggt tttgtttttt gtagg
//
LOCUS       DAOMAA010000516         1673 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_79825_length_1673_cov_3.134116, whole genome shotgun sequence.
ACCESSION   DAOMAA010000516 DAOMAA010000000
VERSION     DAOMAA010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79825_length_1673_cov_3.134116"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1673
                     /locus_tag="PLO59_07215"
     CDS             <1..>1673
                     /locus_tag="PLO59_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00932.1"
                     /translation="NGIRFNNQVTCYILNNKFNLIQGNGVIAGVAGTCISLTNHEATY
                     LNIANNYFDNFDIGITAGLLTDIDLNITNNYFNTTAKFANSQATVGIRILRTPMLNLG
                     NSPKYIQGNIIKHSHKGIFSRLAQGLTIQNNTIEFDPADIAANAVYRYGIHIIGGGNT
                     GYGLHNIESNWIYKTGSVFNLADEKLAAINIQSSGKNFVLSNLINKMGIGVYFGTTTM
                     SNTVHCNEMNDCLTGVRFDASDIGNQGTPGPNGISQGNKWNYSCANCYALKSVNSTTA
                     PTWYVESSAPVNTLPSYPAPNNMFPLGIVAIDNNNILPATDCNIPGPTCPPLRMADIV
                     SGNGDFANMSADERYRALNEVFRILNVDTFYMYMGLPSDIILQNFYDSAYFTNMGMLT
                     IVDELIADSINFVAGILNSWISPINHFEENAAMVNDLYLTCCENGDCIFDSTQYVILY
                     NIASENPCSGGDAVFKAREMLDLMFDDYLTEPAARFANSQSEQVLPDINVYPNPAKNY
                     LAIEINNLSENFIWSYTWSNLLGKTEDKREGLTAPSFKINTSDIKQGVY"
BASE COUNT          532 a          332 c          329 g          480 t
ORIGIN      
        1 taatggcatt aggtttaata accaggtaac ctgttatatt ctaaacaata aatttaacct
       61 gatacaaggc aacggtgtaa tagctggtgt ggcaggtacc tgcataagcc ttaccaacca
      121 tgaagctacg tacttaaaca ttgccaataa ttatttcgac aattttgata ttggcataac
      181 agcaggttta ctaaccgaca ttgacctgaa cataaccaac aactatttta acacaacagc
      241 caaatttgcc aattcccagg caacagtagg cataaggatt ttgcgtacgc ccatgcttaa
      301 tttaggtaat tcgcccaagt acatacaagg taatattatt aagcacagcc ataaaggtat
      361 ttttagccga ctggcacagg ggcttaccat acaaaacaat accattgaat ttgaccctgc
      421 cgatattgcc gccaatgccg tttaccgcta tggcatacat ataattgggg gtggcaacac
      481 cggatacggt ttacataaca tcgaaagcaa ctggatatat aaaaccggca gtgtgtttaa
      541 cttagccgat gaaaaacttg cagccataaa catacaatcg agtggcaaaa actttgtatt
      601 gagtaacctg ataaacaaaa tgggcattgg ggtttacttt ggcaccacca ctatgtctaa
      661 tacagtgcat tgcaacgaaa tgaacgactg ccttacagga gtgcgctttg atgcctccga
      721 tattggcaac cagggcacac cgggacctaa cggaatatcg caaggcaata aatggaatta
      781 cagttgcgct aattgttatg cactaaagag tgtaaacagt accactgcac caacctggta
      841 tgtggagagt tctgctccgg tgaatacatt gccttcttac cctgctccaa ataatatgtt
      901 tcctcttggt attgttgcca ttgataataa caacatatta ccagctactg attgtaatat
      961 acctggccct acctgcccgc ccctgcgcat ggctgatatt gtttcaggta atggtgattt
     1021 tgccaacatg agtgctgatg aacgctatcg ggcccttaac gaagtgtttc ggatattaaa
     1081 tgtcgacacc ttttatatgt atatgggctt accgagcgac atcatactgc aaaactttta
     1141 tgacagcgcc tactttacca atatgggtat gttaaccatt gtggatgagc ttattgccga
     1201 ttcaatcaat tttgtagcag gcatacttaa tagctggata agtcctataa atcactttga
     1261 agaaaatgct gctatggtta atgatttgta tttaacttgt tgcgaaaatg gagattgcat
     1321 tttcgattcc acacagtatg ttattttgta caacattgct tccgaaaatc cttgcagtgg
     1381 gggagatgct gttttcaaag caagagaaat gcttgactta atgtttgatg attacttaac
     1441 tgaacctgct gcaaggtttg caaatagcca aagtgaacag gtattacccg atataaatgt
     1501 ttatcctaat cctgcaaaaa attatttagc aatagagatt aataatttaa gcgaaaattt
     1561 tatctggagt tacacatgga gcaatttgct tggcaaaaca gaagataaaa gggaagggct
     1621 tactgcaccc agctttaaaa taaatacatc tgatattaaa cagggtgtgt aca
//
LOCUS       DAOMAA010000517         1672 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_79927_length_1672_cov_2.894249, whole genome shotgun sequence.
ACCESSION   DAOMAA010000517 DAOMAA010000000
VERSION     DAOMAA010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79927_length_1672_cov_2.894249"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..590
                     /locus_tag="PLO59_07220"
     CDS             156..590
                     /locus_tag="PLO59_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HQV00933.1"
                     /translation="MFSKACEYGIRSAIFITEQSLNGRKTSLKDVAKAIKSPEAYTSK
                     ILQQLVRNSLIKSDKGPTGGFSIEAKNLDKIKLSAIVTAIDGNKVYTGCGLGLSMCNE
                     KKPCPVHYQFKAIREDLKNMLETTTIKSLAQGFEMGLTFLKH"
     gene            640..1044
                     /locus_tag="PLO59_07225"
     CDS             640..1044
                     /locus_tag="PLO59_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HQV00934.1"
                     /translation="MDIKTQKSDINNLEDIKIFVNSFYDKVRTDDVLATIFNDVIKDK
                     WPQHLEKMYRFWQTVLLEEHTYFGSPFLPHAKLPVNAAHFERWVKLFSETIDMLFEGE
                     KANRAKWQGDRMATMFLSKIEYYRNNSSIPLI"
     gene            1041..1490
                     /locus_tag="PLO59_07230"
     CDS             1041..1490
                     /locus_tag="PLO59_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00935.1"
                     /translation="MITVRMPVAAIVTFVWIGYVCSISFMEAWLKFTAPGVTLPIGLS
                     IGRLVFNALNKVEWIFALSVLGNIIYAKQYKVTFNSWYLITISILLLQTIWLLPALDA
                     RAEQYVQGQTPPASNLHYYFVVLEVIKVTGLSLFGISQFRNNKQLST"
     gene            1524..>1672
                     /locus_tag="PLO59_07235"
     CDS             1524..>1672
                     /locus_tag="PLO59_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF542 domain-containing protein"
                     /protein_id="HQV00936.1"
                     /translation="MNITKDTIIGNLVANDYRTASVFKQNGIDFCCNGNRTIEDACVQ
                     KQKNT"
BASE COUNT          594 a          294 c          291 g          493 t
ORIGIN      
        1 agttaacgac ctgcgtgttg attgtcctga gcatcaatta caaactgtat aagcacctaa
       61 ctttttagca gccatataaa tgtaaaatta tagtggactt ttttgtccat tattaaaatt
      121 tatttctatc tttgtcgcat taaatacacg gtgcgatgtt ttcaaaagca tgtgagtacg
      181 gaattcgttc cgcaattttt ataaccgagc aatcactaaa cggccgcaaa accagtttaa
      241 aagatgtagc caaagccatt aaatcgcccg aagcatacac gtcaaaaata ctgcaacaat
      301 tggtgcgtaa cagccttatt aaatctgata aaggccctac gggtggtttt tcaattgaag
      361 ccaaaaacct ggataagatt aagctcagtg ccatagtaac tgcaattgat ggaaataagg
      421 tttacaccgg ctgcggactg ggcttaagta tgtgcaatga aaaaaaacct tgtcccgtac
      481 attatcaatt taaagccata cgcgaagatt taaaaaacat gctcgaaaca actaccataa
      541 aatcgttggc acaaggattt gaaatgggat taaccttttt aaagcattaa ttttttcaga
      601 taaatacata agcttatcaa aaataaaaca caatctaaaa tggacatcaa aacacaaaag
      661 agtgatataa ataatctgga agacataaaa atatttgtga atagctttta cgataaagta
      721 agaactgatg atgtgcttgc aaccatattt aatgatgtaa taaaggataa atggccacaa
      781 catttggaaa aaatgtaccg attttggcaa acagtactac ttgaagagca tacctatttt
      841 ggtagtccgt ttttgccaca tgcaaaactt ccggtaaatg ccgctcattt cgaaagatgg
      901 gttaaacttt ttagcgaaac tattgacatg ctatttgaag gagaaaaagc aaaccgtgcc
      961 aaatggcagg gcgatagaat ggcaaccatg ttcctgtcaa aaattgaata ttaccgaaac
     1021 aattcttcaa taccattaat atgattacag taaggatgcc cgtagctgct atagttacat
     1081 ttgtttggat aggttatgta tgctctatta gttttatgga agcctggcta aaatttacgg
     1141 cacccggtgt aactttacct attggcctta gtatcggccg cttggtgttt aatgcattaa
     1201 ataaagttga atggattttt gcgctatcag tattaggaaa tataatttat gcaaaacagt
     1261 ataaagtaac attcaatagt tggtacctga taactatttc aattttatta ctacaaacta
     1321 tttggctttt acctgcatta gatgctagag ctgaacaata tgtgcaaggt caaactccac
     1381 ccgcttcaaa cctgcactac tactttgttg ttttagaggt aataaaagtt acaggtttaa
     1441 gtctgtttgg catatcacag tttcgaaaca acaagcaact aagcacttaa ataaaatatt
     1501 atttacataa aaactttaaa atcatgaaca ttaccaaaga caccattata ggcaatctgg
     1561 ttgcaaacga ctaccgtact gcatccgttt ttaaacaaaa cggaattgat ttttgttgca
     1621 acggcaatcg cactatcgaa gatgcctgtg tacagaaaca aaaaaataca ga
//
LOCUS       DAOMAA010000518         1669 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_80229_length_1669_cov_2.430607, whole genome shotgun sequence.
ACCESSION   DAOMAA010000518 DAOMAA010000000
VERSION     DAOMAA010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80229_length_1669_cov_2.430607"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..>1669)
                     /locus_tag="PLO59_07240"
     CDS             complement(7..>1669)
                     /locus_tag="PLO59_07240"
                     /inference="COORDINATES: protein motif:HMM:NF024972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HQV00937.1"
                     /translation="TELRSYRLALACTGEYANFHGGTKPTVLAAMVTSMNRVNGVYER
                     EVCIRMNLVPNDTLLIFLDAATDGYDNNSGGAMLSQNQTKCVNIIGLNNFDIGHVFST
                     GGIANLGCVCKSSSKAQGVTGSPSPINDPFDIDYVAHEMGHQFGGNHTFNCETGACQG
                     NRAFTAAYEPGSGITIMAYAGICGSSDNLATHSIATFHPKSFDEITIYSQTGSGNTCA
                     VKTPTGNTPPTMTLQGNYTIPYKTPFRLVANGFDADGDTITYSWEQYDLGPAGSWNVP
                     VGNAPLWRPYLPTLSNTRLCPKLSNILNAVANYTFQSKGELKPDTARTLKFKCTVRDN
                     RAGGGGVTNNDANALVTLTVVNTSSLGFRVTSPNTTGISWPALSWQTITWDVAGTTAN
                     NINCANVNIWLSTDGGLTFPHLQAWQVPNNGSYSCVILNQQSVQCRLMIEGDGNVFFD
                     INDKNFTIGVPTAIYESELSHFVSVTPNPSSGNFLFTLNANKIAGDVTIKILDVNGRL
                     IKQEQLYVDASNNLVQIDLNTVANGVYQALIISDSGVATKRLLKQ"
BASE COUNT          477 a          360 c          337 g          495 t
ORIGIN      
        1 caaattttat tgcttaagca gtcgtttggt tgctacacca ctatcgctaa taatcaatgc
       61 ttgataaaca ccattggcaa cagtgtttaa gtctatttga accaaattgt tactggcatc
      121 aacatatagt tgctcttgtt ttattagcct gccattaacg tctaaaattt taatggttac
      181 atctccggca attttatttg cgttaagcgt aaacagaaaa ttgcctgatg atggattggg
      241 tgtaacactt acaaagtgcg acaactcact ttcataaatt gccgtaggca cacctatggt
      301 aaaattcttg tcgttaatat caaaaaacac attaccatcg ccttctatca tcaaacgaca
      361 ttgcacactt tgctgattta aaattacaca tgagtaggaa ccattgtttg gtacctgcca
      421 ggcttgcaga tgcggaaacg taaggccacc atcggtacta agccatatat ttacattggc
      481 acaattaata ttattggcag tagtacctgc cacatcccaa gtaatggttt gccacgataa
      541 tgcaggccag ctaatgcctg ttgtgtttgg gctggtaact ctaaagccta acgaagatgt
      601 attgaccacc gtaagcgtaa ccaatgcatt tgcatcgtta ttggtaactc cacctccacc
      661 ggcacggttg tcacgtacgg tacatttaaa ctttaaggtt cgtgctgtat caggttttag
      721 ttcgccttta ctttggaatg tgtaatttgc cacagcattt aaaatattag ataacttggg
      781 gcataaacgc gtgttagata atgttggtaa atatgggcgc cataacggag cattaccaac
      841 aggcacattc cacgatccgg ccggccctaa atcgtactgc tcccaactgt aagtaatggt
      901 atcgccatcg gcatcaaatc cattggctac caacctgaat ggtgttttgt aaggtatggt
      961 gtaattgcct tgcaaagtca tggttggtgg cgtgttgcct gtaggtgttt ttactgcaca
     1021 ggtattacca ctaccggttt gtgagtaaat ggtaatttca tcgaaacttt ttggatgaaa
     1081 tgttgcaatg ctatgcgttg ctaaattgtc gcttgagcca caaatacctg cataagccat
     1141 tatggttata ccactgcccg gctcatatgc cgcagtaaat gcgcggtttc cctgacaagc
     1201 accggtttcg caattaaaag tgtggttacc accaaattga tgtcccattt catgtgccac
     1261 ataatcaata tcaaatgggt cgttaatagg cgaaggtgaa ccggttacac cttgtgcttt
     1321 tgaagaagat ttgcatacac aacccaaatt ggcaataccg ccggtactaa aaacatggcc
     1381 aatatcgaag ttattaagac caataatatt aacgcatttg gtttggtttt gacttaacat
     1441 ggcgccacca ctgttgttgt catatccatc ggttgctgca tccagaaaaa tcaatagcgt
     1501 atcattgggc accaaattca ttctgataca aacttcgcgt tcgtaaacgc catttacgcg
     1561 gttcatgcta gtaaccatag ccgctaatac tgtgggtttg gtaccaccat gaaaattggc
     1621 gtactcgcct gtgcaagcca aagccaaacg ataggaacgc aattcagta
//
LOCUS       DAOMAA010000519         1669 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_80254_length_1669_cov_2.067534, whole genome shotgun sequence.
ACCESSION   DAOMAA010000519 DAOMAA010000000
VERSION     DAOMAA010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80254_length_1669_cov_2.067534"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /locus_tag="PLO59_07245"
     CDS             complement(<1..986)
                     /locus_tag="PLO59_07245"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HQV00938.1"
                     /translation="MYPIIKFELSYRLRRPATYAYFFILFILCFFFACSDGVTIGGAT
                     GNVLKNAPFPINQSVMIMTIFSTMIISAVMGVPVYRDFEFNFHEIMFTTPVKKINYLL
                     GRFIGSYLVCVFILSGIIWGLIIGAGVIEYMPWTNVVKKGPFMLMAYINPFINFILPN
                     AFFLGVLFFVSGSLFRSQLAIYVQGVIVFILYLIMINMQKDVDNNELLAIFDPFGYAA
                     SSYQTRYWTTAEKNTLLITLTNLLLYNRLLWIGIALIVAVAAYPFFKFSKTGLFLGKK
                     KKNVSEETATVQSVIIPQVTLQYDFNAQIKQWVYLVVFEFKSVIRSVPFIAIA"
     gene            complement(1019..>1669)
                     /locus_tag="PLO59_07250"
     CDS             complement(1019..>1669)
                     /locus_tag="PLO59_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015030814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQV00939.1"
                     /translation="KTLGYLPQEFGVYPNVSALELLHHVALLKGVTNSRQRNELVNAL
                     LVQTNLFDVRKKKLGGFSGGMKQRFGIAQALIGSPQIIIVDEPTAGLDPTERNRFLNL
                     LSEISEQVIVILSTHIVDDVRELCSQMAIINKGEVMFAGHPEVAIAGLRGKVHTMFTD
                     KETARQIAKEHQIISTKFNTGKIEVHIYSTQPVPGFSPINAGLEDVYFTVINKLSA"
BASE COUNT          564 a          331 c          294 g          480 t
ORIGIN      
        1 gcaatggcaa taaacggtac cgacctgata accgatttaa attcgaaaac gactaaatat
       61 acccactgct taatttgtgc attaaaatca tactgtaaag taacttgcgg aattattacc
      121 gattgaacgg ttgcagtttc ttcacttaca ttttttttct tcttacctaa aaacaaaccc
      181 gttttcgaaa atttaaaaaa tggatatgcc gcaactgcaa ctattaaagc aatgccaatc
      241 cataacaaac ggttatataa aagcaagttg gtaagcgtga tgagcagcgt gtttttttcg
      301 gcagttgtcc aatagcgggt ttgataggat gatgcagcgt atccgaatgg atcgaaaatg
      361 gctaacaact cgttattatc aacatccttt tgcatattaa tcataatcag gtatagtata
      421 aaaacgataa caccctgaac atatatggct aactgactgc ggaacaaact gccacttaca
      481 aaaaacaata cacctaagaa aaaagcatta ggaagtataa aatttataaa agggttaatg
      541 tatgccatca acatgaaagg accctttttt accacattgg tccagggcat atactcaata
      601 acaccggcac caataattaa gccccaaata atgccgctta aaataaaaac gcatacaaga
      661 taggagccaa taaaccggcc taacaggtaa tttatttttt taaccggagt ggtaaacatg
      721 atttcatgaa aattaaattc gaaatcgcga tataccggta cgcccataac tgcactaata
      781 atcatggtgc tgaaaatggt cattatcata acgctttgat tgattggaaa tggtgcattt
      841 ttaagcacat tgcctgttgc accaccaatg gtaactccgt cagaacaggc aaaaaagaaa
      901 cataatataa acaggataaa gaagtaagca taggttgccg gcctgcgtaa ccggtagctt
      961 aattcgaatt ttattatagg atacatgcga taaaatttaa aatgcgtgca ttaatatatt
     1021 aggccgaaag tttgtttata accgtaaagt aaacatcctc taaacctgca ttgatgggac
     1081 taaaacccgg tacaggttgc gtgctgtata tatgtacttc aattttaccg gtattaaatt
     1141 ttgtactaat aatttggtgc tctttggcaa tttgcctggc agtttcttta tctgtaaaca
     1201 tggtatgcac tttaccgcgt aaaccggcaa tggccacctc gggatgcccg gcaaacatca
     1261 cttcgccttt attgatgata gccatttgcg agcacagttc gcgaacatca tcaacaatat
     1321 gtgttgaaag aattacaatt acctgttcgc ttatttcgct tagtaaattt aaaaaacgat
     1381 ttcgttcggt agggtctaaa ccggccgtag gttcatcaac aataataatt tgcgggcttc
     1441 cgattaatgc ttgtgcaatg ccaaagcgct gtttcatacc gccactaaaa ccacctaatt
     1501 tttttttacg cacatcaaat aaattagttt gtaccaaaag ggcatttacc aattcgttgc
     1561 gctgcctgct attagtaaca ccttttaaca atgcaacatg atgcagcaat tccaatgcac
     1621 ttacattggg ataaacacca aactcttggg gcaaataacc caaggtttt
//
LOCUS       DAOMAA010000520         1667 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_80443_length_1667_cov_1.993797, whole genome shotgun sequence.
ACCESSION   DAOMAA010000520 DAOMAA010000000
VERSION     DAOMAA010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80443_length_1667_cov_1.993797"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1232
                     /locus_tag="PLO59_07255"
     CDS             <1..1232
                     /locus_tag="PLO59_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="SusC/RagA family protein"
                     /protein_id="HQV00940.1"
                     /translation="RDDASSRFDKDLRHGLFPSAAFAWRISDETFLQDVSWLSILKLR
                     LGYGVTGQQDINNDYPYIANYSQGNNTAQYQFGSQYYYVLRPDAYDKFIKWEETTTTN
                     AGIDFGFFNGRINGAVDVYRKETKDLIVNAAPAAGTNFGNSVLTNVGSLENEGLEVTL
                     NFIPVATDKITWEIGTNFSTNNGKITKITGASSESEVGIKVGGIQGGINNTIQVHTVG
                     YAPNTFYVYEQIYDANGKPKEGQYIDQNNDGKINENDLVRKHRPDAKYLVGLNTNVTY
                     KNWSLGASLRGEFGHYIYYNVASIRGNLAGMGGSVKALSNLSTDYLNTGFKKEQYFSD
                     YYLRSADFIKLDYVSLAYNFKNLIKDKYNMVASFNVQNVFIISDYAGLDPEVGGGIDQ
                     NIYPRPRIYSINLNFQF"
     gene            1325..>1667
                     /locus_tag="PLO59_07260"
     CDS             1325..>1667
                     /locus_tag="PLO59_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010856255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HQV00941.1"
                     /translation="MTPKYGLNAESVYSKAENYRSVLAKIYGGLILTGNRGPAGSPDF
                     GTGDEGFSSFIRALWNLQELPTDEAVCGWTDPGIPDLNQQTWSNDNSFVTSMYQRIYF
                     EIAMCNEFIRET"
BASE COUNT          547 a          303 c          332 g          485 t
ORIGIN      
        1 tacgtgatga tgcatcatca cgctttgata aagatttgcg ccatggctta tttccatcgg
       61 ccgcatttgc atggcgtata agcgatgaaa catttttgca agatgttagc tggttatcaa
      121 tcttaaaact tcgtttaggg tatggtgtta ccggacagca agacatcaat aacgactacc
      181 catacatagc aaactacagc caaggaaaca atacggctca ataccagttt ggtagccagt
      241 attactatgt gttacgaccc gatgcttatg ataaatttat aaagtgggaa gaaactacta
      301 ctaccaatgc aggtatagat ttcggctttt ttaatggtcg gataaacggt gctgttgatg
      361 tttatagaaa agaaaccaaa gatttaattg tgaatgctgc acctgctgca ggtaccaatt
      421 ttggtaacag tgtacttacc aatgtaggta gtttggagaa tgaaggttta gaagttaccc
      481 ttaactttat accggttgcc acagataaaa ttacatggga aattggaaca aactttagca
      541 ccaacaatgg taaaataact aagattaccg gagcaagttc cgaaagcgaa gttggcatta
      601 aagtgggcgg catacaaggc ggtataaaca ataccattca agtacatacg gtaggctacg
      661 cacccaatac attttatgtt tatgagcaaa tttacgatgc aaacggaaag cctaaagaag
      721 gtcaatacat tgaccaaaac aacgatggaa aaattaatga aaatgatttg gtgcgtaagc
      781 accgccccga tgccaaatat ttagttggtt taaataccaa tgttacctac aaaaactggt
      841 cgttgggcgc aagtttacgt ggcgagtttg gtcactatat ttattacaac gttgcttcta
      901 tcagaggaaa tttggcaggc atgggtggct cggttaaagc acttagcaat ctatcaaccg
      961 attacttaaa taccggattt aaaaaagagc aatatttttc tgattactac ttacgctcag
     1021 ccgattttat taaactggac tatgtaagtt tagcctacaa ctttaaaaac ttaattaaag
     1081 ataaatacaa tatggttgct tcgtttaatg ttcaaaatgt atttatcatt tctgattatg
     1141 ccggcctcga cccagaagtt ggtggcggta ttgatcaaaa catataccca cgcccacgta
     1201 tttactcaat caatttaaac tttcaatttt aatagctata aaaatgaata cgaatttcaa
     1261 atctaaagca atagccatag cctgtttaac attgatgttt acatcatgtt acaaggattt
     1321 ggacatgacc cctaaatacg gtttaaatgc cgaatcggtt tatagcaaag ccgaaaacta
     1381 ccgaagcgtt ttggcaaaaa tatatggtgg actgatactt accggtaaca gaggcccggc
     1441 aggttcgccc gattttggaa caggtgatga aggtttttcg agttttatcc gtgccttatg
     1501 gaatttacaa gaattaccta ccgatgaagc agtatgcggc tggactgacc ctggtattcc
     1561 tgatttaaat caacaaacct ggagtaatga taacagtttt gttaccagta tgtaccaacg
     1621 catttatttc gaaattgcca tgtgtaatga atttatacgc gaaacag
//
LOCUS       DAOMAA010000521         1665 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_80610_length_1665_cov_2.958385, whole genome shotgun sequence.
ACCESSION   DAOMAA010000521 DAOMAA010000000
VERSION     DAOMAA010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80610_length_1665_cov_2.958385"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            277..1410
                     /locus_tag="PLO59_07265"
     CDS             277..1410
                     /locus_tag="PLO59_07265"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008238106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HQV00942.1"
                     /translation="MNSNTLNRTLGILGGGQLGKMLLQKAADFNITAHVIDPDSNAPS
                     KDLCAKFINASFKDYDAVMQFGKHCDIITVEIEHVNTEALSELEKWGKKVYPQPAILK
                     MVQDKGLQKLFYQEHNIPTAPFYLVESKSEIKTDKFPLIQKTRTAGYDGKGVKKLNNV
                     ADVQIAFDEACVIETYIPFQKEIAVIVARNESGEVSCFPAVEMQFNSEANLVELLFSP
                     ADIKEVIASKAHLIATKLINQLQLVGILAVEFFVTTDGDVLVNEIAPRPHNSGHHTIE
                     GNVTSQYEQHLRAIFNLPLGETAIVNPAAMINVLGEKNYTGEAKYEGLHELLKIDGAY
                     LHLYGKKITKPFRKMGHVTVVNKSVDAAITNAKLALQTIKVVS"
     gene            complement(1458..>1665)
                     /locus_tag="PLO59_07270"
     CDS             complement(1458..>1665)
                     /locus_tag="PLO59_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020530576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HQV00943.1"
                     /translation="RIQFVGGLRGLGELERRVNSGEMKVAFALFPVSMKQLIDIADSG
                     NIMPPKVTWFEPKLRSGLVLYELA"
BASE COUNT          542 a          302 c          331 g          490 t
ORIGIN      
        1 aggctttttg tcagcgtaag tttcgtttat gtcggcagca atttgtgcca cacgttgttg
       61 aatatcgttg gctgcgatta atacctcaaa agttttatcg tgtatggtta cttcttgcat
      121 ataaatgttt gccgtatcaa taacaccaat tattgcatac tgtttttaag gctggcgaat
      181 atagttgttt tgtctttgcc tataaatgtt gtttggctat aaatgctgtt taaactaaaa
      241 gcgtacataa aagtttttta aacctatcat tgcaccatga attcaaatac tttgaaccgc
      301 accttaggca tattaggcgg tggacaattg ggaaaaatgt tgttacaaaa agcagccgat
      361 tttaatatta ctgcccatgt tattgacccc gatagcaatg cgcccagtaa agatttatgt
      421 gccaaattta ttaatgcaag ttttaaggat tatgatgccg taatgcagtt tggcaaacac
      481 tgcgatatta ttaccgttga aattgaacat gtaaacacag aagcactttc agaacttgaa
      541 aaatggggta aaaaagtata tccgcaaccg gctattttaa aaatggtgca ggataaaggt
      601 ttgcaaaaac tgttttacca ggagcataat ataccaacag caccgtttta tttggtcgaa
      661 tcgaaatcag aaataaaaac cgataaattt ccgctgatac aaaaaacgcg tacagccggt
      721 tatgatggca aaggcgttaa aaaattaaat aatgtagccg atgttcagat agcttttgac
      781 gaggcatgtg ttatcgaaac ctacatacca tttcagaaag agatagctgt aatagttgca
      841 cgcaatgaaa gtggagaagt gtcctgtttt ccggctgtag aaatgcaatt taacagtgaa
      901 gcaaacttag ttgagttgtt gttttcgcct gctgatataa aagaagtaat tgcaagcaag
      961 gcgcatttaa tagcaaccaa attaataaat caattgcaac tggttggcat attagccgtt
     1021 gagttttttg taacaactga tggtgatgta ttggttaatg aaattgcacc acggccacat
     1081 aacagtggcc atcacacgat tgagggtaat gttacatcgc aatacgaaca acacctgcgg
     1141 gctatattta atttgccgtt aggcgaaact gcaattgtaa atcctgctgc catgattaat
     1201 gtattaggcg aaaaaaatta tacaggcgaa gccaaatatg aaggcttaca tgaactgctg
     1261 aaaattgacg gtgcctacct acatttgtat ggcaaaaaaa taacaaagcc attccgcaaa
     1321 atggggcatg taacggtagt aaacaaaagt gtggatgcgg caattaccaa tgcaaaactt
     1381 gccttgcaaa ccattaaagt agtatcctga ttcatcaggt tttacgcata aaaaaaggct
     1441 gcatgagcag ccttttttta tgctaattca tataacacca aaccactacg cagttttggt
     1501 tcgaaccagg ttactttagg tggcattata ttgccactat cggcaatgtc aataagttgt
     1561 ttcatgctaa cagggaaaag tgcaaatgcc actttcattt cgccactgtt taccctgcgt
     1621 tctaattcgc ccaagccacg taagccaccc acaaattgta tgcgt
//
LOCUS       DAOMAA010000522         1663 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_80798_length_1663_cov_3.029851, whole genome shotgun sequence.
ACCESSION   DAOMAA010000522 DAOMAA010000000
VERSION     DAOMAA010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80798_length_1663_cov_3.029851"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..>1663
                     /locus_tag="PLO59_07275"
     CDS             46..>1663
                     /locus_tag="PLO59_07275"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HQV00944.1"
                     /translation="MGDTKKDIMWRVWLLYSGVCLFALLIIAQVIRIKFVEGQTWIAK
                     ADSLELRMVTINPLRGNVFASDGSLLATSKPIYELRIDTKAEAFKGALFNSKIDSLSQ
                     GLATILGDRTAVQYKMLLKQARVEGERYFLLKRNVSYKEYKAIQQLPLFNKGRFKSGL
                     RVITDSERGLPYGVLAARTIGYAKATVKPVGIEGAFNTALSGVVGKRIEQRMAGGVYK
                     PINDENEIEPQDGMDVVSTIDINIQDVAEHALNKQLSKYNAAYGCVVLMEVNTGDVKA
                     IANLKRGADGKYYEAFNYAIGMGSEPGSTFKLASIITALDDNLIDITDTVDVGNGVSM
                     YAGLEMKDSHLGSGKITVQHAFELSSNVGISKVITEAYAKNPKRFTDKLSAMYLDKPL
                     QLQLSGEAKPRIPKPADVNQKFGWSKVTLPFMSIGYETKLAPIQTLTFYNAVANNGKM
                     VKPRFVKEIRQKGTLVKSFETEVLVDSICSSATIAKAKKMMEGVVKQGTATNLKFEAI
                     KIAGKTGTARMAYGGKGYVSNDSIRYQASFC"
BASE COUNT          548 a          283 c          377 g          455 t
ORIGIN      
        1 cctcgtaaaa tagtaactgt aaattaatac accatcaatt aaacaatggg cgataccaaa
       61 aaagatatta tgtggcgcgt ttggctgtta tacagtggtg tatgcttgtt tgcgctttta
      121 attattgcac aggtaatacg cataaagttt gttgaaggac aaacctggat agccaaggcc
      181 gatagtttgg aattgcgcat ggtaaccata aacccattac gcggaaatgt ttttgcaagc
      241 gatggtagtt tgctggctac atcaaaacct atttatgaat tacgaattga caccaaagct
      301 gaagcattta aaggtgcact ttttaacagt aaaattgatt cattatcgca aggcttagca
      361 accatattag gagacaggac tgctgtgcag tataaaatgc ttttgaaaca agcccgtgtt
      421 gaaggcgagc gctacttttt attgaaacgg aatgttagtt acaaagaata caaagcaata
      481 cagcaattac cgctatttaa caaaggtcga tttaaaagtg gtttacgcgt tataaccgat
      541 agtgagcgcg gcttgccata tggcgtgtta gctgcacgca ccattggtta cgccaaagct
      601 actgtaaagc ctgttggaat tgaaggtgct tttaatacgg cactaagtgg tgtggtaggc
      661 aaacgtatcg aacaacgcat ggccggtggt gtgtataaac caattaatga cgaaaatgaa
      721 attgaaccgc aagatggtat ggatgtggtt tcaaccattg acataaacat acaggatgtg
      781 gccgaacatg cacttaataa gcaattatca aagtacaatg ctgcctatgg atgtgtagtg
      841 ctgatggaag taaatacggg cgatgtaaag gccatagcca atttgaaacg gggtgcagat
      901 ggtaaatatt atgaagcatt taattatgca attggtatgg gctctgaacc aggtagtact
      961 tttaaactgg cttcaattat aacagcgctt gatgataatt taattgatat aaccgataca
     1021 gtagatgtag gtaatggcgt gtctatgtat gccggcctcg aaatgaaaga ttcgcattta
     1081 ggcagtggta aaattacagt gcaacatgca ttcgagttat catcaaacgt aggcatatcg
     1141 aaggttataa ccgaggctta tgctaaaaat ccgaagcgtt ttaccgataa actgtcggcc
     1201 atgtatttag acaaaccgtt acagttacag ctaagtggtg aagcaaaacc acgcatacct
     1261 aaacctgctg atgtaaatca aaagtttggc tggtcaaaag ttacacttcc ttttatgtca
     1321 ataggatatg aaaccaagtt agcacctatt caaacactaa cgttttataa tgcagttgct
     1381 aataatggta aaatggttaa accacgtttt gtgaaagaaa tcaggcagaa gggcacattg
     1441 gtaaaatcgt tcgaaaccga agtgttggtt gactctatat gcagcagcgc aacaattgcc
     1501 aaggcaaaaa aaatgatgga aggagttgtg aaacagggca ctgcaaccaa ccttaaattt
     1561 gaggctataa aaattgcagg caaaacaggt actgcgcgta tggcttatgg tggcaaggga
     1621 tatgtgtcca atgattctat cagatatcag gcttcatttt gtg
//
LOCUS       DAOMAA010000523         1662 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_80909_length_1662_cov_2.592408, whole genome shotgun sequence.
ACCESSION   DAOMAA010000523 DAOMAA010000000
VERSION     DAOMAA010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80909_length_1662_cov_2.592408"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="PLO59_07280"
     CDS             <1..318
                     /locus_tag="PLO59_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00945.1"
                     /translation="GRCRYLNYLCRIIMLKSARLLLLTLGALGIGSLLLALLIGSSTA
                     GPDTLLSWWSGTTDPEALEVLLRLRLPRALAAFGTGAATNSIIDIKYTVRFILIGELY
                     NGV"
     gene            complement(477..1244)
                     /locus_tag="PLO59_07285"
     CDS             complement(477..1244)
                     /locus_tag="PLO59_07285"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HQV00946.1"
                     /translation="MRKLIVIIAMLSCSGCYINSSVMFKTPKNYPYSIDQTIGNLEYR
                     IAPNDIIEFSIYNNDGYKLIEPMMATTTTTSAARIPNDAVNDAINFTVDVEGYVRLPQ
                     IGKVKIKDLTKQEAEKLVEEKYAIYYKNPFSIITVINRRVMVFTGTGGRGTVVNLKNE
                     NTTLIEALAAAGGVTETGKAYKIKLVRGDTRNPQIMLIDLSTVEGLKQSNLLLQANDI
                     IYVEPTPQISQGILKELAPIMAIISSVVVVAVFIRTL"
     gene            complement(1390..>1662)
                     /locus_tag="PLO59_07290"
     CDS             complement(1390..>1662)
                     /locus_tag="PLO59_07290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQV00947.1"
                     /translation="VSLIEAQAASKAIVSTQVGGIENVVIPGKSALLSTSNDVNAFAQ
                     NLITLVNDDMLRDQMSNWGLNHVNSQFNYTRLVSDMTQLYHKLLGI"
BASE COUNT          467 a          321 c          325 g          549 t
ORIGIN      
        1 gggcgctgca gatatctcaa ttacctttgc cggatcatca tgctgaaatc ggcacgtttg
       61 ctattgctga ccctgggcgc actcggcatc gggtcgctgc tgcttgccct gctgatcggc
      121 agcagcaccg ccggaccaga tactctgctg agctggtggt caggaaccac agatcccgaa
      181 gcgcttgagg ttctgctccg tctgcgcctg ccgcgggcac tcgccgcttt tggtaccgga
      241 gcagctacta attcaattat tgatatcaaa tacactgttc gctttatttt gattggcgag
      301 ctttataatg gagtttgatt cgaaaacggg ttgtgtatat cgtaagtata tgagtgccgt
      361 tagagccgac acaaacataa acagtattac ccaaatgaaa ttaccccggg caatagaaac
      421 aaagagtttt aaatcgaaat cttcgttaag actggtaatt ttttttttgg gcattcttac
      481 aaggtgcgta taaatacagc aacaaccact acactactaa taatggccat aataggggcc
      541 agttctttaa gtatgccttg cgatatttgt ggtgtaggtt ctacgtatat aatatcgtta
      601 gcttgtaata gcaggttaga ttgtttaagt ccttcaaccg tacttaaatc aattagcata
      661 atttgcggat tacgtgtatc gcctctaacc aatttaattt tataagcttt acctgtttcg
      721 gtaacaccac ctgccgcagc taaggcttca attaaggttg tgttttcgtt ttttaaatta
      781 acaacggtac ctctgccacc tgtaccggta aataccataa cgcgtctgtt aattacagtt
      841 attatactaa atggattttt ataatagatg gcatattttt cttctactaa tttttcagcc
      901 tcctgtttgg ttaagtcttt aattttcact tttccaattt gtggcaacct aacataacct
      961 tccacatcaa ctgtaaagtt tatggcatcg ttaacggcat cgttgggtat gcgggctgcc
     1021 gaagtggtag tggtagttgc catcatgggt tctattaatt tataaccatc attgttataa
     1081 atgctgaatt cgattatatc attcggggct atacgatatt ccagattacc tatggtttga
     1141 tcaatagagt aggggtaatt tttaggggtt ttaaacatta cactgctatt gatgtaacat
     1201 ccactacaag atagcatggc aataataaca attaacttac gcatatagtt ttactaatag
     1261 agggggtaaa tgtataagaa ttatataaat ggttcagcaa cagatataga tacagattgt
     1321 aatagctaaa aaaaatattt actttttgta cttcaatttt ttagttgaaa attaatattg
     1381 gttaaagcat tatatgccta aaagtttgtg atacaattgc gtcatatcgc taacaagcct
     1441 tgtataatta aattgtgaat taacatggtt taggccccaa ttgctcattt gatcccgcag
     1501 catatcgtca tttacaagcg taattaaatt ttgtgcaaag gcatttacat cgttcgatgt
     1561 cgatagtagc gctgatttac ccggtataac cacattttcg atgccaccca cctgggttga
     1621 tacgattgct tttgaagctg cctgtgcttc gattaaactt ac
//
LOCUS       DAOMAA010000524         1661 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_80991_length_1661_cov_3.580946, whole genome shotgun sequence.
ACCESSION   DAOMAA010000524 DAOMAA010000000
VERSION     DAOMAA010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80991_length_1661_cov_3.580946"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..>1661)
                     /locus_tag="PLO59_07295"
     CDS             complement(56..>1661)
                     /locus_tag="PLO59_07295"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQV00948.1"
                     /translation="KPNANNTILVSRKQSLFENFVTLSSYVFVLFFSTACILYFIYKL
                     INNQFKINFNFKNRIQLTVTSLIMLMMFSAGTITTINLINSYNNSQNQKIIEKINSVL
                     LIIENETRSRPFNRNQIETNYQYKFAEISSSLNADFNIYDTLGMLIYTTQPKVFELDI
                     LGQHMHRNAYNELAVNPKLNLVQHENIGNLNYLAAYEPIRNHANKTIAYLNVPYFKTE
                     ADLHAEVSDLIKVLTNIYVPIFLLSVILTLFFSRKLARPLQLIQINLSKVRLDQSNPL
                     IEWDKKDEIGALVGEYNRMVLELKSSAEALAQSERETAWREMARQVAHEIKNPLTPMK
                     LSVQFLQRALKDKSDDLEKLVKRTSDNLVVQIDTLSHIASEFSNFAKMPTANNAVIDL
                     QLVLKNTSELYDAEDETKIIFHQSENDMLVFADRDQLLRVFSNLLKNALQAITNPKRG
                     IITVTVSADNDMYMVAVSDNGSGIPTDQRDKIFMPNFTTKTTGSGLGLAMSKQIIDSA
                     AGKMWFETEIDKGTTFFVSLPKHIAQ"
BASE COUNT          481 a          278 c          309 g          593 t
ORIGIN      
        1 aagtattcaa tcatccgtat gcttttttac aaagcgcgct aatatagaac aaactttatt
       61 gtgctatgtg tttgggtaac gatacaaaaa aagtagtgcc tttatctatt tcggtttcga
      121 accacatttt gccagctgca ctgtcaatta tttgtttcga catggctaag cctaagcccg
      181 agcccgttgt ttttgtagta aaattgggca taaaaatttt gtcgcgttgg tcggtaggta
      241 tgccgctgcc attatcgcta acggcaacca tatacatatc attatcggcc gaaacggtta
      301 ccgttatgat accacgtttg gggttagtta tggcttgtaa tgcattttta agtaagttgc
      361 tgaatacacg taacaactga tctctatcgg caaaaaccaa catgtcattt tccgattgat
      421 gaaagataat ttttgtttcg tcttcggcat catataattc tgatgtgttt tttaatacca
      481 gttgtaaatc aataacagcg ttatttgcag taggcatttt tgcaaagttg ctaaactctg
      541 atgctatatg acttaatgta tcaatttgta ctactaaatt atcactggtt cgttttacta
      601 atttttctaa atcatcgctt ttatctttta atgcacgttg taaaaactgt acacttaatt
      661 tcataggtgt tagagggttt tttatttcat gcgcaacctg ccttgccatt tcgcgccagg
      721 ctgtttcgcg ctcgctttgt gctaatgctt cggcactgct tttaagttcg agtaccatac
      781 ggttgtactc gcctacaagt gcgcctattt catctttttt atcccattca ataagcgggt
      841 tcgattggtc gagacgtacc ttgcttaagt tgatttgtat cagttgtaat ggacgtgcca
      901 gcttgcgcga aaaaaacaat gtaagtatta cactcagcaa aaaaatgggc acataaatat
      961 tggtgagcac tttaatcaaa tccgaaactt cggcatgtaa atcggcttcg gttttaaagt
     1021 agggtacatt taaatatgca attgttttat tggcatgatt gcgaataggc tcatatgcag
     1081 ccaggtaatt taaattgcca atattttcat gttgtactaa atttaatttc ggatttacag
     1141 ccagttcatt gtatgcattt ctatgcatgt gttgccccaa aatatccaat tcaaaaactt
     1201 taggctgtgt ggtataaata agcataccca atgtatcgta aatgttaaag tcggcattga
     1261 gcgaactgct tatttcggca aatttgtatt gataattggt ttcaatttga ttgcggttaa
     1321 atgggcgact gcgggtttcg ttttcaataa tcagtaaaac cgaatttatt ttttctataa
     1381 ttttttgatt ttgtgagttg ttgtaactat taatcaggtt aatggttgta atagtaccag
     1441 cactaaacat catcaacata attaatgatg ttactgtaag ctggatgcgg tttttaaaat
     1501 taaaattgat tttaaactga ttgtttatca gtttataaat aaagtaaagg atgcatgcag
     1561 ttgaaaaaaa caaaacaaat acataacttg atagcgtaac aaaattttcg aacaagcttt
     1621 gttttcggct aaccaatatg gtgttgtttg cattaggttt a
//
LOCUS       DAOMAA010000525         1661 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_81017_length_1661_cov_2.775841, whole genome shotgun sequence.
ACCESSION   DAOMAA010000525 DAOMAA010000000
VERSION     DAOMAA010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81017_length_1661_cov_2.775841"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..>1661
                     /locus_tag="PLO59_07300"
     CDS             152..>1661
                     /locus_tag="PLO59_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HQV00949.1"
                     /translation="MTLDSKAPKGIPLAEKWKSSKFNYKLVNPANKRKYDIIVIGTGL
                     AGASAAASCAELGYNVKTFCFQDSPRRAHSIAAQGGINAAKNYRNDGDSVYRLFYDTI
                     KGGDYRAREANVYRLAEVSVNIIDQCVAQGVPFAREYGGLLDNRSFGGAQVSRTFYAR
                     GQTGQQLLLGAYSAMNRQIKAGMIKSYTRHEMLDVVVKDGKTVGVIVRDMVTGEISRH
                     AGHAVLLCTGGYGNVFYLTTYARGSNATAIWRAHKKGAYFGNPCYTQIHPTCIPVSGD
                     HQSKLTLMSESLRNDGRVWVPKAKGDKRKPEAIPENERDYYLERKYPSFGNLVPRDIA
                     SRAAKEQCDAGHGVGETGLAVYLDFADAIKRMGQKAVEAKYGNLFDMYKQITGENPYT
                     TAMRIYPAVHYTMGGLWVDYNLMTTIPGLYALGEANFSDHGANRLGASALMQGLADGY
                     FVLPATIGDYLADVERTAVSTNSPEFDEAEKAVRQRIDTLLNIKGKKTVHEFQ"
BASE COUNT          505 a          341 c          370 g          445 t
ORIGIN      
        1 attacaccaa gcttattata aatgtaggct atttattttc agtaattata ccggcaactt
       61 ttgctgccat gcctgtttat tttttgataa aaggataatt tacattctat catttacttc
      121 tcaaattttc agtataaatt aatatcatcc tatgacacta gattcgaaag cgcccaaagg
      181 aattccatta gccgaaaaat ggaaatcctc taagttcaat tataaattgg taaacccggc
      241 caacaagcgc aaatatgata tcatagtaat cggaacgggt ttggcaggtg catcagcagc
      301 agcctcatgt gccgagttag gttacaatgt aaaaacattt tgttttcagg attcgcccag
      361 gcgtgcccat agtatagctg cccaaggtgg tattaatgca gcaaaaaatt atcgtaacga
      421 tggcgatagt gtttatcgtt tgttttacga taccattaaa ggtggcgatt accgcgcacg
      481 cgaagcaaac gtatatcgcc ttgccgaagt gagcgtaaac ataattgatc aatgtgtagc
      541 ccaaggtgta ccttttgcac gcgagtatgg cggattgtta gacaaccgtt catttggcgg
      601 tgcacaggta tcgcgtacat tttatgcacg tgggcaaacc ggccaacaat tattattagg
      661 tgcatacagt gcaatgaaca ggcaaataaa agcaggtatg attaaaagtt atacccgcca
      721 cgaaatgctt gatgtagttg taaaagacgg caaaacagta ggtgttattg tgcgcgatat
      781 ggttacgggc gaaatttcgc gtcatgcagg ccatgctgtt ttattatgta caggcggcta
      841 tggtaatgta ttttatttaa ccacctatgc acgcggcagc aatgcaaccg ctatttggcg
      901 ggcacataaa aaaggtgcat actttggcaa cccctgttac acacaaattc acccaacctg
      961 catacccgta agtggcgacc atcaaagcaa acttactttg atgagcgaaa gtttacgtaa
     1021 cgatggacgc gtttgggtac ccaaagcaaa aggcgataaa cgcaaacccg aagccatacc
     1081 cgaaaacgag cgcgattact atttagagcg taaatatcca tcgtttggaa atttagtgcc
     1141 acgcgatatt gcttcgcgtg ctgctaaaga acaatgcgat gccggacatg gcgttggcga
     1201 aaccggattg gcagtttact tagattttgc cgatgcaatt aagcgtatgg gacaaaaagc
     1261 cgtagaggcc aagtatggaa atctgttcga catgtataaa caaattacag gtgaaaatcc
     1321 ctatacaact gccatgcgca tatatcccgc agtgcattat accatgggtg gtttatgggt
     1381 tgattataat ttgatgacta ccatacccgg actttatgca ttgggcgaag ctaattttag
     1441 cgaccatggt gccaaccgtt tgggtgcaag tgcattaatg caaggtttag ccgatggtta
     1501 ttttgtattg cccgccacca ttggcgatta tttagccgat gtagaacgca ctgccgtaag
     1561 caccaacagc cccgagtttg acgaagccga aaaagcagta cgccaacgta ttgatacgtt
     1621 gctgaacata aaaggtaaaa aaaccgtgca tgaatttcaa c
//
LOCUS       DAOMAA010000526         1656 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_81499_length_1656_cov_2.778888, whole genome shotgun sequence.
ACCESSION   DAOMAA010000526 DAOMAA010000000
VERSION     DAOMAA010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81499_length_1656_cov_2.778888"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1656)
                     /locus_tag="PLO59_07305"
     CDS             complement(<1..>1656)
                     /locus_tag="PLO59_07305"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HQV00950.1"
                     /translation="DDDGGNGYYSQLSSFNLVPQSTVYIQVWGYGNNQGNFKICITNP
                     GKLIADSSALPIVLINTLGNTIVNDVKVDATIDIKYQNNGSINYLSDSSYYTGKIGIE
                     KRGASSAGYPQTPYGFELRDTLNNEMEAPLFNMPAESDWNLISNYNDRSLLRNHIAFK
                     LFENMGNYSVRTKLVEVMVDSMYRGIYLLTEKIKRDKNRVNIAKLTTIDTSGNDVTGG
                     YILQQNHWDAFTSFQSNYSPVGHPTFDVHFVYEYPKPDSITAQQRTYIASYVDSLETA
                     LYSTNFADPINGYRKYLDVPSFIDYFIVNELARNNDGFKKSVFFNKDKNTHYSKLKMG
                     PVWDFDWAFKNIWGCNIFESTDGTGWAHHINDCPTDNYSCGWYIRLLQDSTFANQLHC
                     TYENYRSNILDTSAIFSIIDSVHTLVANVQQRHFLRWPILGISGPAPEVNAIAATYAA
                     ELDTLKAWLTQRISWLDANLPGLCNTASINPVKYNAVSCYPNPGNGTFYFANLYASHA
                     WLSVYNLQGALMMEKHLTSNHNSFTLKQPGFYLYKLLIDGNTYT"
BASE COUNT          508 a          308 c          318 g          522 t
ORIGIN      
        1 gtgtaagtat taccatctat tagcaactta tataaataaa aaccgggttg tttaagtgta
       61 aaagagttat ggtttgatgt taggtgcttt tccatcatta atgcaccctg taggttataa
      121 accgataacc atgcatggga agcataaagg ttggcaaagt aaaatgtgcc attgcccgga
      181 ttggggtaac aacttacagc attgtactta accggattaa ttgatgctgt attacataag
      241 ccgggcaagt tggcatctaa ccaactaatg cgttgtgtaa gccatgcttt taaagtatca
      301 agctcggctg cataagttgc tgcaatggca ttaacctcgg gtgcagggcc actgatacct
      361 aatataggcc atcttaaaaa gtgtctttgt tgcacattgg caactaaggt gtgcacactg
      421 tcaattattg aaaaaatagc agaagtatct aaaatatttg aacgatagtt ttcataggtg
      481 caatgtaatt ggttggcaaa agtactgtct tgtaataagc gaatatacca gccacaggag
      541 tagttgtcgg tagggcaatc gtttatatga tgcgcccatc ccgtgccatc ggtactttca
      601 aaaatattgc agccccaaat atttttaaat gcccagtcaa aatcccaaac gggccccatt
      661 tttaatttac tgtaatgggt gtttttatcc ttattaaaaa acacactttt tttaaagcca
      721 tcgttattac gcgccaattc atttacaata aagtaatcaa taaatgatgg tacatctaaa
      781 tatttgcgat aaccatttat cgggtcggca aaattggtac tatataaagc tgtttctaaa
      841 ctatcaacat aagaagcaat atatgtacgt tgctgtgcag ttatactatc gggtttggga
      901 tattcatata caaaatgtac atcaaatgtt ggatggccaa cagggctgta gtttgattga
      961 aaactggtaa atgcatccca atggttttgt tgtaaaatat aaccaccggt tacgtcatta
     1021 ccgctggtgt caatggtagt aagtttggca atgttaacgc ggtttttatc gcgtttaatt
     1081 ttttcggtaa gcagataaat gccacgatac atgctatcaa ccatcacttc aaccaacttg
     1141 gtgcgcacac tgtagttgcc catgttttcg aacaatttaa atgcaatatg atttcgcaat
     1201 aatgaccggt cattataatt tgaaatcagg ttccagtcgc tttcggcagg catattaaat
     1261 aaaggcgctt ccatttcatt gtttaaggta tcgcgtaact caaaaccata aggtgtttgt
     1321 ggatagccgg ccgatgaagc gcctcttttt tcaataccaa ttttgccggt ataataactg
     1381 ctgtcggaca aataatttat actgccattg ttttgatact ttatatcaat ggttgcatct
     1441 acctttacat cgttaacaat ggtattaccc agcgtattaa ttaatacaat gggcaaagcc
     1501 gaactatcgg ctattaattt gcccgggttg gtaatacata ttttaaagtt tccctgattg
     1561 ttgccataac cccaaacctg aatgtacact gtactttgcg gcactaaatt aaatgatgaa
     1621 agttgcgagt agtaaccatt accaccatca tcatcg
//
LOCUS       DAOMAA010000527         1655 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_81601_length_1655_cov_2.318125, whole genome shotgun sequence.
ACCESSION   DAOMAA010000527 DAOMAA010000000
VERSION     DAOMAA010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81601_length_1655_cov_2.318125"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /locus_tag="PLO59_07310"
     CDS             complement(<1..810)
                     /locus_tag="PLO59_07310"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HQV00951.1"
                     /translation="MKTLHHIIISRTDGIGDVILTLPMCGILKSNFPDLKITFLGANY
                     TKEIVACCKHVDAFVSYDDMQQADNAGGFLKALHADAIVHVFPRKEIAYAAKDAQIPV
                     RIGTSHRAYHWISCSDTPNVGRKNSKLHEAQLNIMLLAPLGIETHYQPELLPQYYGFT
                     APHHPDLPNVSTQTFNLIIHPKSNGSAREWGMHQYAKLIEQLPPEKFTIYITGTKAEL
                     DAIALLNTAQWPHVHNVAGLMDVKSLIAFINACNGLLACSTGPLHIAAALGI"
     gene            complement(890..1642)
                     /locus_tag="PLO59_07315"
     CDS             complement(890..1642)
                     /locus_tag="PLO59_07315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020531510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQV00952.1"
                     /translation="MPQISVTVITFNEERNIGRCLQSVIDIADDIVVLDSGSTDNTIA
                     ICKTFNVRVEIAPWQNYSHARNVAASYAKHDWILALDADEALSDELKISIEAFKQNNQ
                     PTFASFNRLTNYCGKWIKHCGWYPDVKIRVYDKTKATYEGIIHEHLNWLPDHVQKLKG
                     NLLHYSYYTVFEHIQQANKFTNLTAKAAYDRGDKSNMIYVVFAPLLKFIKSYFLQLGF
                     LDGYYGFLVCIISAHATFYKYVKLHLLLKQGK"
BASE COUNT          513 a          310 c          320 g          512 t
ORIGIN      
        1 tatgcccaat gctgctgcaa tgtgtaatgg gccggtacta catgccaata atccattaca
       61 tgcatttata aaggcaatga gtgatttaac atccataagg cccgctacgt tatgtacatg
      121 tggccattgt gctgtgttaa gcaatgcaat agcatctaac tcggccttag taccggtaat
      181 gtatattgta aatttttcgg gaggcagttg ttcaatcagt ttggcatact ggtgcatgcc
      241 ccactcgcgt gcactgccat tacttttagg atgaataatt aagttaaatg tttgtgtaga
      301 aacgtttggt aaatccgggt gatgcggagc tgtaaaacca taatactgcg gcaacaattc
      361 gggctggtaa tgggtttcaa tgcctaaggg cgccaatagc ataatattaa gttgtgcctc
      421 atgcagtttc gaatttttac ggcctacgtt tggcgtatcg ctacatgaaa tccaatggta
      481 tgccctatgt gatgtgccaa tacgtacagg tatttgagcg tctttggcag cgtaagcaat
      541 ttcttttctt gggaaaacat gtacaatagc atcggcatgt aatgctttta aaaaaccacc
      601 ggcattatca gcttgttgca tgtcatcata cgatacaaat gcatcaacat gtttacagca
      661 ggctacaatc tctttagtat agttggcacc taaaaaagta attttcaaat ccggaaaatt
      721 actttttaaa ataccgcaca tgggcaaggt aagtattaca tcacctatac catcggtacg
      781 gctaataatt atatgatgta gcgttttcaa aaaacaataa tgtataaagg gtagttttta
      841 atgcgcagca agctaatatt aattgaataa ttgtttgtaa gccaaacagc tatttgcctt
      901 gctttaacaa taagtgcaac ttaacatatt tataaaacgt tgcatgtgcc gatataatgc
      961 aaaccaaaaa gccatagtag ccatctaaaa aacccaactg taaaaaatag cttttaataa
     1021 attttaaaag cggggcaaaa accacataaa tcatattgct tttgtcgcca cggtcgtaag
     1081 cagctttggc cgttaaattg gtaaacttat tagcttgttg tatgtgttca aacacagtgt
     1141 aataactata atgcagcaaa ttgcctttta gtttttgaac atgatcgggg agccagttta
     1201 aatgctcatg tataatgcct tcgtacgttg ctttggtttt atcatacacc cgtattttca
     1261 catcgggata ccagccgcaa tgtttaatcc acttaccaca atagttggtt aaacggttaa
     1321 atgaagcaaa ggtaggttgg ttgttttgct taaaggcctc aatcgaaatt tttaattcat
     1381 cgcttaatgc ctcatcagca tcgagggcta aaatccaatc atgttttgca taagatgcag
     1441 caacattacg agcatgcgaa tagttttgcc acggtgcaat ttctacacgt acattaaacg
     1501 ttttacaaat ggctatggta ttatcggttg agcccgagtc taatactaca atatcgtcag
     1561 caatatcaat aacactttgc aagcatcggc caatgttacg ttcttcatta aaagttatta
     1621 cggttacaga tatttgtggc atggcacaaa gaaat
//
LOCUS       DAOMAA010000528         1653 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_81743_length_1653_cov_3.396120, whole genome shotgun sequence.
ACCESSION   DAOMAA010000528 DAOMAA010000000
VERSION     DAOMAA010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81743_length_1653_cov_3.396120"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /gene="gmhA"
                     /locus_tag="PLO59_07320"
     CDS             <1..430
                     /gene="gmhA"
                     /locus_tag="PLO59_07320"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007242866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HQV00953.1"
                     /translation="NGGSMCDAMHFAEELSGRFRNNRPALAAVSISDASHISCVGNDY
                     GYDKIFSRYIEALGKPNDVLLAISTSGTSKNVLQAIEAAKAQGIKVVGLTGKDGGAMK
                     DLCDIEIRAPHSTYADRAQEIHIKIIHSLIHYIELKLQLA"
     gene            465..>1653
                     /locus_tag="PLO59_07325"
     CDS             465..>1653
                     /locus_tag="PLO59_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HQV00954.1"
                     /translation="MLVKTFGSAVYGVNATTITIETNVSEGIKFYLVGLPDNAIKESQ
                     QRIEAALKNIGFKMPGKKVVINMAPADIRKEGSAYDLTIAIGILMASEQIMQANINEY
                     IIMGELSLDGGLQPVKGALPIAIQARKEGFTGIILPKQNAREAAIVADLKVYGVENLR
                     QVIDFFTGDLTIEPTVVNTQEEFYKQQASNEFDFSDVRGQENIKRALEIAAAGGHNVI
                     LIGPPGAGKTMLAKRLPTILPPLSLQEALETTKIHSVAGKLKKEAALVSVRPFRSPHH
                     TISDVALVGGGGIPQPGEISLAHNGVLFLDELPEFKRTVLEVMRQPLEERSVTISRAK
                     FAIEYPASFMLVASMNPCPCGYYNHPDKDCVCAPGVVQKYLNKISGPLLDRIDLHVEV
                     TPVA"
BASE COUNT          520 a          326 c          370 g          437 t
ORIGIN      
        1 caacggaggc agcatgtgcg atgccatgca ctttgccgag gaacttagtg gccgctttag
       61 aaacaacaga ccggctttgg ctgccgtaag tattagcgat gcttcgcata tttcatgtgt
      121 gggtaatgat tatggttacg ataaaatttt ttcgcgatac attgaggcat tgggcaaacc
      181 caatgatgta ttgttggcaa taagtaccag tggcaccagt aaaaatgttt tgcaagcaat
      241 tgaagcagcc aaagcacaag gcataaaagt agttggcctt accggtaaag atggtggtgc
      301 catgaaagat ttatgcgata ttgaaatacg cgcgccacat agtacctatg ccgaccgtgc
      361 acaagaaata cacattaaaa taattcattc gcttatacat tacatcgaac tgaaattaca
      421 actggcataa acggctttaa tttctgaact aaaatttctg aaccatgtta gttaaaacct
      481 ttggcagtgc agtgtatgga gttaatgcca ctaccattac catcgaaacc aatgtaagcg
      541 aaggcattaa attttattta gtgggtttgc ccgataatgc tattaaagaa tcgcaacaac
      601 gtatagaggc tgcattaaag aatatcggtt ttaaaatgcc cggcaaaaaa gtagtaataa
      661 acatggcccc tgccgatatc agaaaagaag gatcggctta tgatttaacc attgctatag
      721 gcattttaat ggcatcagaa caaataatgc aagccaacat taacgagtat attataatgg
      781 gcgagctgag tttagatggg ggcttgcaac cggttaaagg cgcgttgccc attgccatac
      841 aagcccgtaa agaaggcttt acaggtatta tcttacccaa acaaaatgca cgcgaagcag
      901 ccatagttgc cgacttaaaa gtgtatggcg ttgaaaacct gcgtcaggta attgattttt
      961 ttaccggtga tcttactatt gaaccaaccg ttgtaaacac gcaagaagag ttttataaac
     1021 aacaagcctc aaacgaattt gattttagtg atgtacgcgg acaagaaaat ataaaacgtg
     1081 cgctggaaat tgcagcagcc ggtggtcata atgtgatact gattggtcca ccgggtgcag
     1141 gtaaaaccat gctggccaaa cgtttgccaa ccatattgcc gccattatca ttgcaagaag
     1201 ctttggaaac aacaaaaata cacagtgttg caggcaaact taaaaaggaa gcagctttag
     1261 taagtgtgcg tccattcagg tcgccacatc ataccattag cgatgtagct ttagttggcg
     1321 gtggaggcat accacaacca ggcgaaataa gtttagcaca taatggcgtt ttgtttttag
     1381 acgagttgcc cgagtttaaa cgcaccgttt tagaagtaat gcgccaacct ttagaagaac
     1441 gcagtgtaac catatcgcgc gccaaatttg ctatcgagta tccggcaagt tttatgttgg
     1501 ttgccagtat gaacccatgc ccttgtggtt attacaacca tcccgataag gattgtgttt
     1561 gcgctccggg tgtagtacaa aaatatctga ataaaatatc aggcccgttg ttggatcgta
     1621 ttgacctgca tgtggaagta acgcccgttg cat
//
LOCUS       DAOMAA010000529         1653 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_81748_length_1653_cov_3.080100, whole genome shotgun sequence.
ACCESSION   DAOMAA010000529 DAOMAA010000000
VERSION     DAOMAA010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81748_length_1653_cov_3.080100"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1653)
                     /locus_tag="PLO59_07330"
     CDS             complement(<1..>1653)
                     /locus_tag="PLO59_07330"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HQV00955.1"
                     /translation="QLETAIVHEVGHNWFQGILATNERNEPWLDEGINSFYEMEYFLQ
                     KYPNQNVTDFDAIGTPIANLLGNKFLNAHSYKFNQYQSAARTWSNQPNNLQANSYTQS
                     NYGNIIYAKSAVAIAYLKKYLGNNLFKQCMQQYFLRWQFMHPNTYEFKNVIEDVSQQN
                     VDWFFDEIIATNNLVDYKIKKVSQRPPYTISIKNKTGIAAPVFIDVNYANGTEQICLN
                     GFQKSALIPLQEKPVSIVIDSLNMMMDSYFANNYYNSKQLFKKQTLGLRFYSSYDAKK
                     SLLYYIPLAGWNQYNHWMPGVLIHNQSLVPKPLEFAIMPLYSFATNNLNGSFMFQYKQ
                     ALSLQWQYIAKIKGMQYNFGSTYFNATDQLQQFRFKKIQPSIIIQHRSDVLSKLLQQI
                     SATAYFINRNDVFENGTVPSVKSENFYYQQLDYEIKNNRTFDPWQALLTIENGKNYLK
                     AFTQAQYNFSYKRRQRGITVNAFAGTFIYNENEMQNVNFRMSGFSGMQDYLFNYTYLG
                     RSESEGILSKQFIANDGGFKINTAVGQTNKWLTSLNLQVTIP"
BASE COUNT          485 a          277 c          295 g          596 t
ORIGIN      
        1 cgggtatggt aacttgtaaa ttaagagaag ttaaccactt attggtttgc ccaacggctg
       61 tatttatttt aaagccgcca tcattggcaa taaattgttt gcttaaaatt ccttcgcttt
      121 cgctgcggcc caggtatgtg tagttaaaca aataatcttg cataccgcta aaacctgaca
      181 tccggaaatt tacattctgc atttcgtttt cattataaat aaaggttccg gcaaatgcat
      241 ttaccgttat gccacgttgc ctgcgtttat agctaaagtt atattgcgct tgggtaaatg
      301 ctttcaggta atttttgcca ttctctatag ttaataatgc ctgccaggga tcgaaagttc
      361 tgttgttttt tatctcgtaa tctaattgtt gatagtaaaa attttctgat tttacagaag
      421 gtacagttcc attttcaaac acatcattac ggttaatgaa atacgctgtt gcgctgatct
      481 gctgaagtaa tttgcttaac acatctgacc gatgttgaat aattattgac ggctgaattt
      541 ttttaaaccg gaattgttgt agctgatctg ttgcattaaa ataagtactg ccgaaattat
      601 actgcatacc cttaatcttt gcaatgtatt gccattgcag gctaagcgcc tgtttgtact
      661 gaaacataaa actgccatta aggttattgg tagcaaaact atatagtggc attattgcaa
      721 actctaatgg ttttggaacc aaactttggt tatgaattaa aacaccgggc atccaatggt
      781 tgtattgatt ccatcctgct aagggtatat agtacagtaa tgattttttt gcatcataac
      841 ttgaataaaa acgtaaaccc aatgtttgtt ttttaaacag ctgtttactg ttgtaataat
      901 tgttagcgaa atacgaatcc atcatcatat ttaaactgtc aataacaata cttaccggtt
      961 tttcctgtag tggtataagc gctgactttt ggaaaccatt taaacaaatt tgttccgtac
     1021 cgtttgcgta gtttacatca ataaaaacag gtgcagctat gcctgtttta tttttaatgc
     1081 taattgtata tggtggacgt tgacttacct ttttaatttt ataatcaacc aaattattgg
     1141 tggcaataat ttcatcaaaa aaccaatcaa cattttgttg gcttacatct tcaatcacat
     1201 ttttaaactc atacgtattt gggtgcataa attgccaacg caaaaagtac tgttgcatac
     1261 attgcttaaa caagttgtta cctaagtatt ttttaagata tgcaatggca acagcacttt
     1321 ttgcataaat gatattgcca taatttgatt gtgtatagct atttgcttgt aagttgtttg
     1381 gctgattgct ccaggtgcgt gctgcacttt gatattgatt aaatttataa ctgtgtgcat
     1441 ttaaaaattt attgcctaaa aggttagcaa tgggtgtgcc tatggcatca aagtcggtta
     1501 cattttgatt tggatatttt tgaaggaaat attccatttc ataaaaagag tttatgcctt
     1561 cgtcaagcca gggttcgtta cgttcattgg tagctaaaat gccctgaaac cagttatgac
     1621 ctacttcatg tacgatggct gtttcgagtt gat
//
LOCUS       DAOMAA010000530         1650 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_82069_length_1650_cov_2.477743, whole genome shotgun sequence.
ACCESSION   DAOMAA010000530 DAOMAA010000000
VERSION     DAOMAA010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82069_length_1650_cov_2.477743"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            280..516
                     /locus_tag="PLO59_07335"
     CDS             280..516
                     /locus_tag="PLO59_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HQV00956.1"
                     /translation="MSDIDSKVKKIIVDKLGVDEAEITNESSFTTDLGADSLDTVELI
                     MEFEKEFNISIPDDQAEKIQTVGNAIEYIAANAK"
     gene            547..>1650
                     /gene="fabF"
                     /locus_tag="PLO59_07340"
     CDS             547..>1650
                     /gene="fabF"
                     /locus_tag="PLO59_07340"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HQV00957.1"
                     /translation="MVLKRVVVTGLGAITPLGNSVSEYWNGLINGVSGSAAITRFDAS
                     KFKTQFGCEVKNFDVGQYLDRKEARKMDLFTHFAIASSVQGVADAGIEDNAEINKDRV
                     GVIWGSGIGGLRTFQDEVSAFALSDGTPRFNPFFIPKMIADIAPRLISMRFGFRGPNF
                     TTVSACASSTNALIDAFNYIRLGKADIIISGGSEAAINEAGVGGFNALHAMSTRNDSP
                     QTASRPFDKDRDGFVLGEGGGCIVLEEYEHAVKRGAKIYAEVKGGGMSADAYHMTAPH
                     PEGLGAYNVMLNVLSDAEMQPHEIDYINVHGTSTPIGDPQEIKAIQRAFGDEAFKLNI
                     SSTKSMHGHLLGGAGAIESIATILAVKNDIVPPT"
BASE COUNT          522 a          289 c          335 g          504 t
ORIGIN      
        1 atgtagctaa aacagccaca tataaaaggt tctttttcat cttatttgat tttagtttat
       61 gtgagcaaat ctatatgctt actttcaaaa taatttgtta aaaattactt tcgtttttaa
      121 aagtatcgcc aataaaaaag ttctgtattt tgttactgct attaaaaaac ttaaatacta
      181 cagcacagat tctcgaacaa aaaaaatttg cgcataacca taaattatta cttttgcccg
      241 ctctcaaaaa ttagcaacta aaatttaaaa actcatacta tgtcagatat tgactcaaaa
      301 gttaaaaaaa taatcgttga caaattaggt gttgacgaag ctgaaataac caacgaatca
      361 agctttacca ccgatttagg tgctgattca ttagatacag ttgagcttat tatggagttt
      421 gaaaaagaat tcaatatttc tattccagac gatcaagctg aaaaaattca aacagtaggt
      481 aacgctatcg aatatattgc cgctaacgcg aagtaatctt acttcttctt ttatcagaaa
      541 tacattatgg tgttaaaacg cgttgttgtt acaggtttgg gtgcgattac gcctttaggt
      601 aattcggttt ctgaatattg gaacggatta attaatggcg tatcggggtc ggctgcaatc
      661 acacgttttg atgcttcaaa gtttaaaaca caatttggtt gtgaagtaaa aaactttgat
      721 gtaggccaat atcttgaccg taaagaagca cgtaaaatgg acttgtttac ccattttgcc
      781 attgcatcca gtgtacaagg tgtagccgat gccggtattg aagataatgc tgaaataaat
      841 aaagatcgtg taggggttat ttggggctcg ggtattggag gtttacgtac tttccaggac
      901 gaagtatcgg cttttgcctt aagcgatggc acaccccgct ttaacccatt ctttattcct
      961 aaaatgatag ccgatattgc acccagatta atttcgatgc gtttcggttt cagggggcct
     1021 aattttacaa cagtaagtgc ctgtgcaagt agtaccaatg cacttattga tgcctttaat
     1081 tatatccgat taggcaaggc cgatataata attagtggtg gcagcgaagc agccattaac
     1141 gaagccggag tgggtggctt taatgcattg catgccatga gcacccgtaa cgattcgcca
     1201 caaacagcat cacgaccttt tgataaggac agagatggtt ttgtgttagg cgaaggcggt
     1261 ggttgtattg ttttagaaga atatgaacat gctgtaaagc gtggtgcaaa aatttatgcc
     1321 gaagtaaaag gtggtggtat gagtgctgat gcctatcaca tgacagcacc acatcccgaa
     1381 ggtttgggcg catacaacgt aatgttaaat gtgttgagcg atgccgaaat gcaaccgcat
     1441 gaaattgact atattaatgt gcatggcact tcaacaccta ttggcgatcc acaagaaata
     1501 aaagctatac aacgtgcctt tggtgatgaa gcttttaaac taaatatcag ttcaaccaaa
     1561 tcaatgcatg gtcatttatt aggtggtgct ggtgccattg agtctattgc taccatattg
     1621 gctgttaaaa acgatattgt tccaccaacc
//
LOCUS       DAOMAA010000531         1644 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_82626_length_1644_cov_2.302077, whole genome shotgun sequence.
ACCESSION   DAOMAA010000531 DAOMAA010000000
VERSION     DAOMAA010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82626_length_1644_cov_2.302077"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /gene="moaCB"
                     /locus_tag="PLO59_07345"
     CDS             <1..849
                     /gene="moaCB"
                     /locus_tag="PLO59_07345"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010521676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional molybdenum cofactor biosynthesis
                     protein MoaC/MoaB"
                     /protein_id="HQV00958.1"
                     /translation="VSNAHTIALINSNTIPKGNIFEASKIAGLFAVKNTSSAIPDCHP
                     LPIESTTITHRIEGLQIIIEVAVKTIYRTGVEVEAMHGASIVALTIYDMLKPVDKGIE
                     ICNIKLLKKTGGKSQYNDVLNSTLKAAVVVCSDSIAAGNKTDFAGKAIITTLEKHNIH
                     VADYSIIADEIKLITEKAILLSEQQYDLVIFTGGTGLSPRDVTPEALRPLLTREIPGI
                     AEAARSYGMEHMPYAMLSRSLAGLRDNTLILALPGSTRGAQESMDALFPYLLHIFKVL
                     QHTTHA"
     gene            959..>1644
                     /gene="bshA"
                     /locus_tag="PLO59_07350"
     CDS             959..>1644
                     /gene="bshA"
                     /locus_tag="PLO59_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HQV00959.1"
                     /translation="MKIGIVCYPTFGGSGVVATELGKALAENGHQIHFITYNQPVRLE
                     HFHSNISYHEVSIADYPLFEYVPYETALTSKMVDVVKFEQLDILHVHYAIPHASAAYM
                     AKLILKQQGINIPIVTTLHGTDITLVGKNPNYLPVVTFAMNMSDGLTAVSNSLRMDTY
                     ANFDVQKEIEVIHNFIDLKRFSKKHKDHFKRAIEPNNEKLVVHTSNFRKVKRVQDVIY
                     VFDQIRKAIP"
BASE COUNT          572 a          325 c          288 g          459 t
ORIGIN      
        1 gttagcaatg ctcataccat tgcattaatt aatagtaaca ccatacccaa aggcaacatt
       61 tttgaagcca gtaaaattgc aggacttttt gctgtaaaaa ataccagctc tgccataccc
      121 gattgccatc cgttgcccat agagtcaacc actataacgc atcgtattga agggttacag
      181 attattatcg aagttgccgt aaaaaccatt tatcgcaccg gtgtcgaagt tgaagccatg
      241 catggtgcat ccatagttgc acttactata tatgacatgc ttaaacctgt agataaaggc
      301 atagaaattt gcaacattaa attgcttaaa aaaaccggag gtaaaagcca atacaacgat
      361 gtattaaaca gcacattaaa agcagcagtt gtagtttgct ccgatagtat tgcagccgga
      421 aataaaactg attttgcagg caaagcaatt attacaacct tagaaaaaca taacatccat
      481 gttgccgact acagcataat tgctgacgaa attaaattga taacagaaaa agcaatacta
      541 ctttctgaac aacaatatga cttggttatt tttacaggtg gtacaggttt gtcgccacgc
      601 gatgtaacac cggaagcatt acgcccgtta ttgacgcgcg aaatacccgg cattgccgaa
      661 gctgcacgaa gttatggtat ggaacacatg ccttatgcca tgttatcgcg tagtttagcc
      721 ggcttacgcg acaatacact tatactagca ttaccgggtt cgacccgtgg tgcacaggaa
      781 agcatggatg cacttttccc atatctgcta catatattta aagtgttaca acatactacg
      841 catgcctaac ttcttttatt aaatcactat caaatacttt acacttcttt atatgaagta
      901 caataatttt aaacaacaaa cgattgcgta aatcagcaat tcattaaaaa taaataacat
      961 gaaaattggc atcgtttgct atcccacatt tggaggttcg ggcgtggtag ctactgagtt
     1021 aggaaaagca ttagctgaaa acggacatca aattcatttt atcacctata atcaacctgt
     1081 acgattagag cattttcact ctaacatatc gtaccatgaa gttagcatag ccgattaccc
     1141 actttttgag tatgtaccct atgaaacagc attaaccagt aaaatggtag atgtagtaaa
     1201 gtttgagcag ttagatatac ttcatgtaca ttatgccata ccgcatgcat cggcagcata
     1261 catggctaaa cttattttaa aacaacaagg cataaacata cccattgtta ctacgttaca
     1321 cggtaccgat attacacttg taggaaaaaa tccgaattac ttgcccgttg taacttttgc
     1381 catgaatatg agtgatggat taacggccgt atcaaacagt ttgcgtatgg atacgtatgc
     1441 aaactttgat gtacaaaaag aaattgaagt aatacacaac tttattgact taaagcggtt
     1501 ttcgaaaaaa cataaagatc attttaagcg tgccattgaa cccaacaacg aaaaattagt
     1561 agtacacaca tcaaactttc ggaaagttaa aagagtacag gatgtaattt atgttttcga
     1621 tcaaataaga aaagccatac caag
//
LOCUS       DAOMAA010000532         1639 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_83084_length_1639_cov_3.327020, whole genome shotgun sequence.
ACCESSION   DAOMAA010000532 DAOMAA010000000
VERSION     DAOMAA010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83084_length_1639_cov_3.327020"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="PLO59_07355"
     CDS             <1..349
                     /locus_tag="PLO59_07355"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV00960.1"
                     /translation="WAIYRNRAIEDLMLNIKISKLTFQGLDENKNLSLQLYPNPANDA
                     AIIKVHALETIKQFSYEIITAQGQKVSSGLAEPNQSMAEVKIETKSLPAGLYICRILV
                     NDTVITRSLSVTH"
     gene            complement(624..>1639)
                     /locus_tag="PLO59_07360"
     CDS             complement(624..>1639)
                     /locus_tag="PLO59_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008509149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HQV00961.1"
                     /translation="RELAIQIAESFSNYGANTSLKCCVIFGGVNQYSQVEALRHGVDV
                     LIATPGRLLDLMQQKFVHLQHLQFFVLDEADRMLDMGFVNDVKKVIAVLPKKRQSLFF
                     SATMPQAILKLADTILQNPAKVAVTPENTTAETVSQTLYYVDKENKKNLLQYIIKNKN
                     IERTLVFTRTKHGADRVAKDLSKSNIAAQAIHGNKSQNARQHALQNFKNHLTRVLVAT
                     DIAARGIDIDDLTHVINYDLPNIPESYVHRIGRTGRAGADGIAISFCDVDERPFLDDI
                     NKLIGFKIPVVEHPYPMQKLVPEKKSQSNPTHKKSVGSAQRNPHKRHQNTNKAKPQNN
                     RIY"
BASE COUNT          520 a          317 c          287 g          515 t
ORIGIN      
        1 ctgggctatt tatcgtaacc gtgctattga agacttaatg cttaacatta aaatttcgaa
       61 acttactttt cagggattgg acgaaaacaa aaatttgtcg ctacaacttt atccaaatcc
      121 tgcaaacgat gctgccataa taaaagtaca tgctttagaa accataaaac aatttagtta
      181 tgaaattata actgcacaag gccaaaaagt aagcagcggt ttggcagaac caaatcaaag
      241 tatggccgaa gtaaaaatag aaaccaaaag cttgcctgcc ggtttatata tctgccgcat
      301 acttgttaac gataccgtta ttacccgcag cctaagcgta acacattaat ttagctgcaa
      361 aacgttcgtt taaataaaaa aggctgctta attaagcagc cttttttatt taagacttaa
      421 tgaagcacat gataaaataa cttagcttaa tttatatcaa accacggcac atatttatgc
      481 acaaaattac caaacaaaat gtaaccctta tagtaaatac attttacgga agtataggca
      541 gaataaacaa acacttgcat gctgttgcct tttagttttt ctatctaaac gttagcataa
      601 ataaaaaagc aatcaatgac ctattaatag atgcgattgt tttgaggttt tgccttgttc
      661 gtgttttgat gacgtttatg cggattacgc tgtgccgaac ctaccgattt tttatgcgtt
      721 ggatttgatt gggatttttt ttcaggcact aatttttgca tgggatatgg atgttcaaca
      781 acaggtattt taaaaccaat taacttatta atatcgtcta aaaatgggcg ttcgtccaca
      841 tcgcaaaacg aaattgcaat accatcggcc ccggccctac cggttcggcc gattcgatgc
      901 acataacttt cgggaatatt gggcaaatcg taatttatta catgggtcaa atcgtcaata
      961 tcaataccac gtgcagcaat atcggttgct actaatacac gcgttaaatg atttttaaaa
     1021 ttttgcaatg catgttgtct ggcgttctgc gatttgttac cgtgtatagc ctgcgctgca
     1081 atatttgatt ttgataaatc tttagctacc ctgtcggcac catgcttggt acgggtaaat
     1141 accaaagtgc gctcaatatt tttattttta ataatgtact gcaacaagtt ttttttattt
     1201 tccttatcaa cataatataa ggtttgactt acggtttcgg cagtagtgtt ttcgggtgtt
     1261 acggctactt ttgccggatt ttgcaatatc gtatctgcaa gcttcaaaat agcctggggc
     1321 atggtagccg aaaaaaacaa cgattgcctt ttcttaggta aaacagcaat tacctttttt
     1381 acatcattaa caaaacccat gtctaacatg cgatcggctt cgtctaaaac aaaaaactga
     1441 agatgttgca ggtgcacaaa tttttgctgc atcaaatcga gtaagcggcc tggtgttgca
     1501 attaaaacat caacaccatg acgcaacgct tcaacctggc tgtactgatt tacacctcca
     1561 aaaattacgc aacactttaa tgatgtattt gcaccgtagt tcgaaaaact ttctgcaatt
     1621 tgaattgcta actcacgcg
//
LOCUS       DAOMAA010000533         1638 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_83185_length_1638_cov_3.466203, whole genome shotgun sequence.
ACCESSION   DAOMAA010000533 DAOMAA010000000
VERSION     DAOMAA010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83185_length_1638_cov_3.466203"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..820)
                     /locus_tag="PLO59_07365"
     CDS             complement(71..820)
                     /locus_tag="PLO59_07365"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HQV00962.1"
                     /translation="MQHIKAIIVEDEPYNRQLLLAKLEQFYPQIRIVDACSNGQEALL
                     LVKKYNPELVFLDIEMPVMNGFEFLKQYGPHDFEVIFVTSYDKYALQAIKFSALDFIL
                     KPIDNDELIAAVNQFLFKHKNGIDQKSLLQNLVGNMQTPKPENYRLALSTLDGTLFLN
                     TDEIIRCEADSNYTKFVLHKRANLLASKTLKEFDELLTEHHFVRIHKSHLVNRKYIRK
                     LSADGYVILHDESQVEVSRRRIAEVKIQLGL"
     gene            997..>1638
                     /locus_tag="PLO59_07370"
     CDS             997..>1638
                     /locus_tag="PLO59_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQV00963.1"
                     /translation="MQSGKINVQTENIFPIIKKFLYSDHEIFLRELVSNAVDASQKIK
                     TLAGAGVYSGTLGELAVQVAVNKDAKTITITDNGIGMTADEVERYINQIAFSGAEEFI
                     EKYKAESATNTIIGHFGLGFYSAFMVADKVEIITKSYQDAPAVHWVCDGTTNYELTET
                     TKDDRGTVITLHINEDSVEFLENARIKTLLDKYGKFLPVEVKFEDKVINNTQPA"
BASE COUNT          564 a          297 c          292 g          485 t
ORIGIN      
        1 aaactttttt tggtaatgcc acccatttga agcaaattac catataggtt tgcttgttta
       61 tgtacaaaaa ttataagcct aattgaattt ttacctcggc aatacgcctg cgcgaaacct
      121 caacctgcga ctcgtcatgc aaaattacat aaccatcggc acttaatttt ctgatgtact
      181 tacggttaac caaatgcgat ttatgaatac gaacaaaatg atgttcggtt agcaactcgt
      241 caaactcttt taatgttttt gatgccaata aattagcacg tttatgcaaa acaaacttag
      301 tatagttgct atcggcttcg catctgataa tttcatctgt atttaaaaat aaagtgccat
      361 ctaaagtgga aagtgccaac cggtaatttt caggcttggg cgtttgcata ttgccaacta
      421 aattctgcaa caacgacttt tggtcaattc catttttatg tttaaacaga aactgattta
      481 cagcagcaat tagttcatca ttatcaatgg gttttaaaat aaaatctaaa gcgctaaact
      541 taattgcttg taaagcatac ttatcgtaag aggttacaaa aattacttca aaatcatgcg
      601 ggccatattg ttttaaaaac tcaaaaccat tcataaccgg catttcaatg tctaaaaaaa
      661 ccaattcagg attatatttt tttaccaata gcagggcctc ttgtccgttg ctgcatgcat
      721 caacaatccg tatttgtgga taaaactgct cgagctttgc caacaataac tgccggttat
      781 atggctcatc ttcaacaatt atggctttta tgtgttgcat gtattaggca tttcatcaat
      841 aagcaaacat aaaataatta gcagcaataa gtgtgcaaaa tgaagtaatg actatgacaa
      901 gcaactatta gatattgaaa atttggcagt aaaaaagcgt tggcactcca tttgaaaagt
      961 gtgctgaaat aaattattaa atcaaaaaaa ttatacatgc aatcaggtaa aataaatgtt
     1021 caaacagaga acatatttcc aatcattaaa aagtttttat acagcgacca cgaaatattt
     1081 ttgcgcgagc tggtttcaaa tgccgttgat gcatctcaaa aaataaaaac attggccggt
     1141 gccggtgttt acagcggtac tttaggcgaa ttagcggttc aggttgccgt aaataaagat
     1201 gctaaaacca ttaccattac cgataatgga ataggcatga ctgccgatga ggtggagcgg
     1261 tacatcaacc aaatagcatt tagcggtgcc gaagagttta tcgaaaaata taaagccgag
     1321 tcggccacaa ataccattat aggccatttt ggcttaggct tttattcggc atttatggta
     1381 gccgataaag ttgaaataat taccaagagt taccaggatg caccagcagt acattgggtt
     1441 tgtgatggca ctaccaacta cgaactaacc gaaactacta aagacgatcg tggtacggta
     1501 ataaccctgc atataaacga agactcggtt gagtttttag aaaacgcccg tattaaaacc
     1561 ttattagata agtatggtaa gtttttaccg gttgaagtaa agtttgaaga caaggtaata
     1621 aacaatacac agccagcc
//
LOCUS       DAOMAA010000534         1636 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_83383_length_1636_cov_3.056293, whole genome shotgun sequence.
ACCESSION   DAOMAA010000534 DAOMAA010000000
VERSION     DAOMAA010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83383_length_1636_cov_3.056293"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="PLO59_07375"
     CDS             <1..407
                     /locus_tag="PLO59_07375"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HQV00964.1"
                     /translation="IMFNYVLKKQPVPMPGFEFQAPTVALMVSYSCGLITNFSITKFF
                     VFHESTLRTRWQFLRFASVAFFVLGLNYLAMTFLIRQLNWYPTIARTCSALSIGVLSF
                     IMHYWFSFKVKKTDNSSEEIDLLDIPDEDPTI"
     gene            complement(408..743)
                     /locus_tag="PLO59_07380"
     CDS             complement(408..743)
                     /locus_tag="PLO59_07380"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HQV00965.1"
                     /translation="MPYKDKEIERLYYSISEVAKMFDVTISLLRYYDNTFEALSPAKN
                     KKGNRLFTPEDVSYLRGIFYLTRQKGYTLESAKKMMADKSSTIKDEVNLLETLKSLRA
                     FLTELKSQL"
     gene            866..>1636
                     /locus_tag="PLO59_07385"
     CDS             866..>1636
                     /locus_tag="PLO59_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00966.1"
                     /translation="MNTFFNKTRFLLVGFLWFVSNVNGQQYQWAKKIGSIGHDVANCV
                     VTDLQGNTYTVGSFEGTIDFDPTPNFELLNPMGNTDAYIVKYNAAGIYQWSKSISGLN
                     NEIATAVSINKSGVMAIVGYFNGTVDFNPDTTQTHWLTAKGSQDIFTVVLDSAGNFLW
                     AAAQGGNGIDVALAVFIDDSNTVYNAGYFEKTADFNPDTNATFNLTALADFDGFATKL
                     VNGVLIWAMPLTNNKDLEIRNIEVVADKMYLSGITENTT"
BASE COUNT          516 a          263 c          307 g          550 t
ORIGIN      
        1 ttatcatgtt taactacgtg cttaaaaagc agcctgtacc tatgcccggt ttcgagtttc
       61 aggcaccaac tgtggctttg atggtgtcct atagttgtgg tttgattact aattttagca
      121 ttacaaaatt ttttgttttt catgagagca ctttgcgtac acgttggcag tttttaagat
      181 ttgcatcggt ggcttttttc gtattggggc taaactacct ggctatgact tttttaatca
      241 gacaattaaa ctggtaccct actatagcac gtacatgcag tgcattaagt ataggtgttt
      301 taagttttat aatgcattat tggttttcgt ttaaggttaa aaagaccgac aactcatcag
      361 aagaaattga tttattagat atccctgacg aagacccaac tatttaatta aagttgtgat
      421 tttagctcag ttaaaaaggc acgcaaactt tttagtgttt ctaacaaatt tacttcatct
      481 ttaatagttg atgatttatc ggccatcatt ttttttgcac tttcaagggt ataacctttc
      541 tgacgggtta agtaaaaaat gccacgcagg tacgacacat cctcgggcgt aaacaacctg
      601 tttccttttt tgttttttgc aggcgataaa gcttcaaaag tattgtcata ataacgaagt
      661 aacgatatgg ttacatcaaa cattttggca acctcgctta tagagtaata caaacgctca
      721 atctctttat ctttataagg cattattgtt gtaattgaaa tagaaaagat atgtaacgtg
      781 tcaaatatag agcaatatga taaaattaaa cggccgcttt tttaaattat ttacttagaa
      841 atttacattt gcaggcaata taattatgaa tacttttttt aataaaacaa gatttttatt
      901 ggttggattt ttatggttcg tttcaaacgt aaatggtcag caatatcagt gggcaaaaaa
      961 aattggcagc attggccacg atgttgcaaa ctgtgttgta accgatttgc aaggcaatac
     1021 ttatacagtt ggcagctttg aaggcaccat tgattttgat ccgacaccaa attttgaatt
     1081 actaaaccct atgggtaata cagatgctta tatagtaaaa tataatgcgg ccggtatcta
     1141 tcaatggagt aaaagtataa gcggtttaaa taatgaaata gctaccgctg taagcattaa
     1201 taaaagtggg gtaatggcta ttgttggtta ctttaacgga accgtagatt ttaaccctga
     1261 tacaactcaa acacattggc taacagcaaa aggcagtcag gatattttta cggttgtact
     1321 cgattcggca ggcaattttt tatgggctgc tgcacaaggt ggtaatggca ttgatgtagc
     1381 attggccgtt tttattgacg atagtaacac cgtttacaat gcaggctatt ttgaaaaaac
     1441 agccgatttt aatcccgata caaatgcaac ttttaaccta actgcattag ctgattttga
     1501 tggctttgca accaaacttg taaacggtgt gcttatatgg gccatgccat taaccaataa
     1561 caaagattta gaaattagaa atattgaagt ggttgctgat aaaatgtatt taagtggtat
     1621 aaccgaaaat acaacc
//
LOCUS       DAOMAA010000535         1636 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_83394_length_1636_cov_2.818469, whole genome shotgun sequence.
ACCESSION   DAOMAA010000535 DAOMAA010000000
VERSION     DAOMAA010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83394_length_1636_cov_2.818469"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..884
                     /locus_tag="PLO59_07390"
     CDS             93..884
                     /locus_tag="PLO59_07390"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011584341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HQV00967.1"
                     /translation="MTYLQAFIIAIIEGITEFLPVSSTGHMILASSLMDIKDKAFADT
                     FEIVIQLGAILSVLVLYYKRFLVGIDIYLKLFVAFLPTAVVGLLAYKTIKAHLFNPYV
                     VSVSLIVGGVILILLDKWSAQKESVYETTSDISFIDALKIGAIQCFSMIPGVSRSAAT
                     IFGGVFAGFNRKQAAEFSFLLAIPTMFVASAKDLYENKDAITQSNMMLLIFGSVIAFI
                     VALIAVKAFVQYLTKHGFKMFGYYRIIIGLVFLAYALFTGLQLHA"
     gene            complement(912..1292)
                     /locus_tag="PLO59_07395"
     CDS             complement(912..1292)
                     /locus_tag="PLO59_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00968.1"
                     /translation="MQNNFIQYLKYATIVMLLAMALVWIVTRFVMHQPQWFQGFWGIP
                     VFYFLLYAGFHYGALLNTEGKAFVRYYMVASFLKLLLLVAIIVGYALFNKENARGFAI
                     NFLLSYFVFMAFEVYYLRKHFGSR"
     gene            complement(1303..1536)
                     /locus_tag="PLO59_07400"
     CDS             complement(1303..1536)
                     /locus_tag="PLO59_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009196438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HQV00969.1"
                     /translation="METNPQKNQSKKNLPNTFYRYSSLGIQMGATIFLFAFAGVKLDA
                     WLALKFPVFTLVLTLAGVCGTMYYAIKEISKYK"
BASE COUNT          519 a          274 c          304 g          539 t
ORIGIN      
        1 gattaaatat tattaaatat tatttagatt atggataata ttattaaata ctaaactaaa
       61 atttattaat tgtaatttat taagcaacag ctatgaccta tttgcaggcc tttattattg
      121 ctattatcga aggcattact gagtttttgc ccgtttcgtc aacaggccac atgattttgg
      181 cttcgtcatt aatggatatt aaagacaaag cctttgccga tacttttgaa attgtaatac
      241 agttaggtgc tatacttagc gtgttggtac tttattataa aaggtttttg gtggggattg
      301 atatttactt aaaactgttt gtggcttttt taccaactgc tgttgtaggc ttattagcat
      361 ataaaaccat taaagcacat ttgtttaatc cgtatgtagt aagtgtgtcg cttatagtag
      421 gtggcgttat tcttattttg ttagataagt ggagtgccca aaaggaatcg gtttacgaaa
      481 ccacatcaga cataagtttt atagatgctt taaaaattgg agccatacaa tgttttagca
      541 tgataccggg tgtaagccgt tcggccgcca ctatttttgg cggtgtgttt gctggcttta
      601 atagaaagca ggctgccgag ttttcgtttt tattggccat acctaccatg tttgtggctt
      661 cggctaaaga cctttacgaa aacaaagatg ccattacaca aagcaatatg atgttactta
      721 tttttggtag tgttatagct tttattgtgg cacttatagc agtaaaagca tttgtacagt
      781 acttaaccaa acatggtttt aaaatgtttg gctactatcg tataattatt ggtttggtgt
      841 ttttagcgta tgctttgttt accggtttgc aattgcatgc atagtgtttt cgaaatgcga
      901 aaaggcaaac gttatctgga gccaaaatgc ttacgcaaat aataaacctc aaaagccata
      961 aatacaaaat agctaagcaa aaagtttatg gcaaagccgc gtgcattttc tttattaaaa
     1021 agggcatagc caacaataat ggctacaagt aaaagcaatt ttaaaaacga ggccaccata
     1081 taatatcgca caaatgcttt gccttcggta tttagtaaag caccatagtg gaaacctgca
     1141 tacagtaaaa agtaaaaaac aggtatgccc caaaagccct gaaaccattg tggctgatgc
     1201 ataacaaaac gggtaacaat ccaaaccaat gccatagcta aaagcataac tatagttgca
     1261 tacttaaggt attgaataaa gttgttttgc atggttaatg ggttatttgt atttgcttat
     1321 ttctttaatg gcataataca tagtgccaca aacacctgct aacgttagta ccaatgtgaa
     1381 taccggaaat tttaaggcca gccaagcatc taacttaaca cctgcaaagg caaacaaaaa
     1441 aatggtagca cccatttgta tgcctaatga agagtagcgg taaaaggtat taggcagatt
     1501 tttctttgat tgatttttct gcgggtttgt ctccaaagtt tagctcctta atattagcac
     1561 ccatattaca attgcctgta aaagttgcgc ctacttcaac cactaattta ccggttacga
     1621 tatcgccatt aacctt
//
LOCUS       DAOMAA010000536         1633 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_83728_length_1633_cov_3.223067, whole genome shotgun sequence.
ACCESSION   DAOMAA010000536 DAOMAA010000000
VERSION     DAOMAA010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83728_length_1633_cov_3.223067"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..144
                     /locus_tag="PLO59_07405"
     CDS             <1..144
                     /locus_tag="PLO59_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HQV00970.1"
                     /translation="IRAGVSLLIAALSAKGKSVIHNIEQIDRGYERIDERLAALGAEI
                     ERI"
     gene            249..770
                     /locus_tag="PLO59_07410"
     CDS             249..770
                     /locus_tag="PLO59_07410"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HQV00971.1"
                     /translation="MKKVAITLCLLLATGLTMAQKKSVSTGIEVGQKAIDLKFKSPDG
                     KEIALSSLKGKVVLLDFWASWCGPCRRENPAVVAAYNKYKDEKIGKAKGFTVYSVSLD
                     QEKQKWVDGIKADGLSWPYHVSDLAGWHSKAAQLYSVNSIPTNWLLDANGVIVARGLR
                     GDALIQAIEKLRK"
     gene            919..1605
                     /gene="ung"
                     /locus_tag="PLO59_07415"
     CDS             919..1605
                     /gene="ung"
                     /locus_tag="PLO59_07415"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HQV00972.1"
                     /translation="MTQINIEPSWMHAMQAEFDKPYFKQLIAALKTEKAAQEIIYPPG
                     NQIFAAFNQTPIPKVKVVLLGQDPYHGPGQANGLCFSVNKGIDLPPSLQNIFKELSDD
                     CQIPLPTNGDLTAWAQQGVLLINSLLTVKAHQPLSHQYIGWEQFTDAVIRTLSEQCNG
                     LVFLLWGKYAQSKTVLIDTFKHFILTAPHPSPLSAYRGFLGCKHFTKANHILLQQNVA
                     EIRWDAITQA"
BASE COUNT          536 a          302 c          325 g          470 t
ORIGIN      
        1 atacgtgcag gtgtttcatt actaattgca gcacttagcg ccaaaggcaa aagtgtaata
       61 cataacattg agcaaattga ccgtggctat gagcgtatag atgaacgcct ggcagccttg
      121 ggtgcagaaa tagaacgtat ataagtacgg cattataaac tattgttaaa acaagttgcc
      181 tcaaacattg attattcaat ttcaatcgtt aggagtatat ttgaagcctt atcaattaaa
      241 aataacctat gaaaaaagta gcaattacat tatgcctgtt gttggcaacc ggattaacta
      301 tggcgcaaaa gaaatcggtt tcaaccggca tcgaagtagg tcaaaaagct atagacctaa
      361 aatttaaatc gcccgatggt aaagaaatag ccttatcatc cctaaaaggg aaagtggttt
      421 tattagattt ttgggcatcg tggtgtggcc catgccgcag agaaaacccg gctgtagttg
      481 ctgcatacaa taaatataaa gacgaaaaaa ttggtaaagc caaaggattt acggtttaca
      541 gcgtatcatt agaccaggaa aaacaaaagt gggttgatgg cattaaagcc gatggtttgt
      601 catggccgta tcatgtttct gatttagcgg gttggcattc gaaagctgcg caactttata
      661 gtgttaattc tatacctact aactggttgt tagatgcaaa tggtgtaatt gttgcaagag
      721 gtttacgcgg tgatgcatta attcaagcaa tcgaaaaatt aagaaaataa ggattagggg
      781 taataattat tttcgtacaa ccggctaact gcgtaagctt tagccggttt tttatttgat
      841 acctcaaccc cgtttcattt tcatttttag ggcattatta catccttgca ttaataatta
      901 gccaatagca gataaaaaat gacccaaata aatatcgagc cgagttggat gcatgccatg
      961 caagccgaat ttgacaagcc atattttaag caattaatag ctgctttgaa aaccgaaaaa
     1021 gctgctcagg aaattatata tccacccggc aatcaaattt ttgcagcatt caatcaaacc
     1081 ccaatcccaa aagtaaaagt ggtactattg gggcaagatc cgtatcatgg tccagggcag
     1141 gctaatggcc tatgtttttc tgtaaacaaa ggcattgatt tgccgccatc gcttcaaaat
     1201 atttttaaag aacttagtga cgattgtcaa ataccgctgc caaccaatgg cgatttaact
     1261 gcctgggcgc agcaaggtgt tttgctaata aattcgttgc ttacagttaa agcgcatcaa
     1321 ccattatcac atcaatatat tggatgggag caatttaccg atgctgttat acgcaccctt
     1381 agcgaacagt gtaacggttt ggttttctta ttgtggggca aatacgcaca atcaaaaacg
     1441 gttttaatcg atacgtttaa acattttatt ttaacagcac cacatccatc accactatcg
     1501 gcataccgtg gttttttagg ttgcaaacac tttacaaaag ccaaccatat tttattgcaa
     1561 caaaatgtag ctgaaatacg ttgggatgct attacgcaag catgaattta aaagctacat
     1621 actatgcagc gct
//
LOCUS       DAOMAA010000537         1630 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_83999_length_1630_cov_3.473651, whole genome shotgun sequence.
ACCESSION   DAOMAA010000537 DAOMAA010000000
VERSION     DAOMAA010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83999_length_1630_cov_3.473651"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="PLO59_07420"
     CDS             <1..794
                     /locus_tag="PLO59_07420"
                     /inference="COORDINATES: protein motif:HMM:NF025459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4271 domain-containing protein"
                     /protein_id="HQV00973.1"
                     /translation="SKQQSFAALTDSAFVDTLAVKPEQAIDLLFKNHLLQFDFFYPQT
                     FDKPVSDWFSILLLIVISTVTLLKVFYSKIFFQIFKAFFNNNTANQIIRDENLLVQRA
                     SILLSVICYVVLALFAYNAVIYFHIENRYLGNGFALFLSIAIIIALSYSVKMIVLKAL
                     GSIFYFNKPVTAYIFNIFLINNVLGIVLIPFIILGTYLTANNNLILFAGLALVIIFFI
                     YRLVKGMLLWTGTNSFNLFYLFLYICALEISPLLILSKLLLIQKQ"
     gene            848..1591
                     /locus_tag="PLO59_07425"
     CDS             848..1591
                     /locus_tag="PLO59_07425"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HQV00974.1"
                     /translation="MKVKSILVTQPKPETEKSPYFDLAKKYNLKIDFRSFIHVEGIQA
                     QEFRKQKVNLLDYSAVVLTSKHAADHFFRICTEMRITDVERFKYFCISESTAYYLQKY
                     VQFRKRKIFFGKSTINDLMDLIKKHRKEKFIVPCSDVHKQEIADTLSENSIDFEKAVI
                     YKTVCSDLSDLAKVNYDMLVFFSPSGITSLFKNFPKFKQNNTRIAAFGATTSLAVTDA
                     GLKLDIKAPVPEAPSMTMAIEQYVKKVNK"
BASE COUNT          541 a          276 c          259 g          554 t
ORIGIN      
        1 taagtaaaca acaatcattt gctgccctta ccgattcggc ctttgtagat acccttgcag
       61 taaaaccgga acaagcaatt gatctgttat ttaaaaatca tttactccaa ttcgattttt
      121 tttaccccca aacatttgat aagccggtta gcgattggtt tagcatattg ttgcttatag
      181 ttataagtac ggtaaccttg cttaaagtat tttattcgaa aatatttttt cagattttta
      241 aagctttctt taataataat acagccaacc aaattatacg ggatgaaaat ctcttagttc
      301 aacgtgcatc aatactttta agtgtaattt gttatgttgt gctggcacta tttgcatata
      361 atgccgtaat atattttcat atcgaaaacc ggtacctggg taacggtttt gccctgtttt
      421 taagtattgc tataattatt gccttatcct attcagtaaa aatgatagtt ctaaaagcat
      481 taggttcgat attttacttt aataaaccgg taactgcgta tatatttaat atatttttaa
      541 ttaataatgt tctcggtatc gttttaatac catttattat tttaggaacg tacctaaccg
      601 ctaataataa cctgatacta tttgcagggt tggcattggt aatcatcttt tttatttaca
      661 ggcttgtaaa aggaatgtta ttgtggacgg gtacaaatag cttcaatttg ttttatttat
      721 ttttatacat ttgcgccctc gaaatttcac ccttgttgat tttatcaaaa ctactgttga
      781 tacaaaaaca ataagcccat ttcggaataa gattgttgaa ccgcgactat taaataaaac
      841 ctgaagcatg aaagtaaaaa gtattctggt tactcagcca aaacctgaaa ctgagaaatc
      901 accttacttc gacctcgcca aaaaatacaa tttaaaaatt gatttcaggt cgttcattca
      961 tgttgaagga attcaggcac aggagttcag aaagcaaaaa gtaaatttac ttgattatag
     1021 cgctgttgta cttaccagta aacatgctgc cgatcacttt tttagaattt gtacagaaat
     1081 gcgaattacg gatgtggaac gttttaaata tttctgcatc agcgaatcaa ctgcatacta
     1141 tttacagaag tacgtacaat ttagaaagcg aaaaatattt tttggtaaat caaccattaa
     1201 cgacttaatg gatctgatta aaaaacatcg taaagaaaaa tttattgttc catgtagcga
     1261 tgtccacaaa caagaaatag ccgatacgct ttcagaaaac agtattgatt tcgaaaaagc
     1321 ggttatttat aaaaccgtat gtagcgattt atcggattta gctaaagtaa actacgatat
     1381 gctggtattt tttagcccgt caggaattac ttcacttttt aaaaactttc ctaagtttaa
     1441 gcaaaacaat acgcgtattg cagcttttgg cgcaaccaca tcattggcag ttaccgatgc
     1501 aggtttaaag cttgatatta aagcaccggt acccgaagca ccttcaatga ctatggccat
     1561 tgaacaatat gtaaaaaagg taaataaata attacaagcc tgcctgcgct gatatttcta
     1621 acattctttt
//
LOCUS       DAOMAA010000538         1623 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_84699_length_1623_cov_2.819515, whole genome shotgun sequence.
ACCESSION   DAOMAA010000538 DAOMAA010000000
VERSION     DAOMAA010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84699_length_1623_cov_2.819515"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="PLO59_07430"
     CDS             <1..402
                     /locus_tag="PLO59_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein ScpA"
                     /protein_id="HQV00975.1"
                     /translation="ERGNVQAELARIALQTQETNYQSELETLTLYKLLTTFEKVMERF
                     QNSSQNNHHTILIAPYSIDKQKDYIVNLIRSRNRCAFADLFGACENKLHAIFNFLALL
                     EMIQAGLVLLVIGEGYNNFWISSPETNFYAN"
     gene            392..1447
                     /locus_tag="PLO59_07435"
     CDS             392..1447
                     /locus_tag="PLO59_07435"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HQV00976.1"
                     /translation="MQTDETSVLKQFSTTKFLLPVGIGLSVAAFMIWRNFDSNAFDKI
                     TWTWTSSLWIALAFVSVLVREMAYMMRIRILTDSHLSWYRSFIVIMLWECSSALAPGI
                     IGGGFFFAIFILSREGVNVGRSISVITFSNFLDGVFLACMVPIVYWIAGADALFSGVQ
                     LQTGLGKSLYYSFWSVYYIMVAYKIFVAYALFVNPYLIKRMLAAVFSLPLLKRFRRGV
                     IETGNQLIIASEDLKTKNLRYWTGALVTTFISWTARYSIVNCIIHAFHGTAPLADFVV
                     YGKQVIMGIILFASPTPGGSGLAEFIFSDFLGQYIQVGLAPSLALLWRLISFYPYIII
                     GAVILPRWVRAKVFNKL"
BASE COUNT          429 a          299 c          353 g          542 t
ORIGIN      
        1 gagcgtggca atgtgcaggc agagttagcg cgtattgcac ttcaaacaca agaaaccaat
       61 taccaaagcg agttagaaac attaacgctt tataaattac taaccacatt cgaaaaggtg
      121 atggagcgtt ttcaaaacag ctcacaaaac aaccaccaca ccatccttat tgccccctac
      181 agtattgaca aacaaaagga ctacatcgta aacctgatac gctcgcgtaa ccggtgtgcc
      241 tttgccgatt tgtttggcgc ttgcgaaaat aaactgcatg ccatttttaa ttttttagca
      301 ttgttagaaa tgattcaggc cggtttagta ttattggtga ttggtgaggg ctacaacaac
      361 ttttggataa gcagccccga aacaaatttt tatgcaaact gatgaaactt cggttttaaa
      421 acaatttagt actaccaaat ttttattgcc tgtaggcatc ggcttgtcag ttgctgcctt
      481 tatgatttgg cgcaactttg acagtaatgc ctttgataaa attacctgga cctggacttc
      541 gtcactatgg atagcgttag cttttgtaag cgtgttggtg cgcgaaatgg cttatatgat
      601 gcgcatccgt attttaaccg attcgcactt atcatggtat cgtagtttta ttgtaataat
      661 gttgtgggag tgctcgtcag cattggcgcc cggcattatt ggtggcggtt ttttctttgc
      721 catatttatt cttagccgtg aaggggttaa cgtaggtcgc agtatttcgg taatcacatt
      781 ctcaaacttt ttagatggtg tgtttctggc atgcatggtg cccatcgtat attggattgc
      841 cggtgccgat gctttgtttt cgggggttca attacaaacg ggtttaggta aaagtttata
      901 ctactcgttt tggagtgtgt attatattat ggttgcctat aaaatatttg ttgcctatgc
      961 gctttttgta aatccgtatt taattaaacg catgctggca gcagtgtttt cattgccatt
     1021 gctcaaacgg ttcagacgcg gtgtaattga aacaggcaat caattaatta ttgcttccga
     1081 agatttaaaa actaaaaact tgcggtattg gacaggagcc ttggttacta cttttatttc
     1141 gtggactgcg cgctactcca tagttaactg catcatacat gcatttcatg gtacagcccc
     1201 attggccgat tttgtggtgt atggcaaaca agtaattatg ggcattatat tatttgccag
     1261 ccctacaccc ggcggtagtg gtttggccga atttattttc agcgattttt tgggtcaata
     1321 catacaagta ggtttagcac cctcgttagc gttgttgtgg cggcttatta gcttttaccc
     1381 ttacattata attggtgcag ttattttacc caggtgggta cgcgccaaag tgtttaataa
     1441 attgtaatca gtaaccagta ccggcatggc ataaaaaaag ctgtaagtat ttacacttac
     1501 agctttgtgg ttagtaactt atagtttttt aaacttggtt gatatattgt atcaattcag
     1561 ttatactgct gctaaaagta aactcggggt ttacattaaa gtaacggtgt tggtcaacgg
     1621 tgt
//
LOCUS       DAOMAA010000539         1622 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_84793_length_1622_cov_3.130185, whole genome shotgun sequence.
ACCESSION   DAOMAA010000539 DAOMAA010000000
VERSION     DAOMAA010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84793_length_1622_cov_3.130185"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..1536
                     /gene="asnB"
                     /locus_tag="PLO59_07440"
     CDS             19..1536
                     /gene="asnB"
                     /locus_tag="PLO59_07440"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008239373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HQV00977.1"
                     /translation="MHRPAVDPQSGGQPLFNSEKTLALAVNGEIYNHMDLRKQLPDYQ
                     FLTHSDCEVILALYQQKGVNFIEDLNGIFAFALYDTQNDSYLIARDHMGIIPLYMGWD
                     KHGNFYVSSELKALEGVCNKIQEFLPGHYWHSKEGTLKKWYQRNWTQYENVKDNKSDI
                     NQLRTALEAAVHRQLMSDVPYGVLLSGGLDSSIISAITKKYAPNRIESNDTQMAWYPQ
                     LHSFAVGLKGSPDLAAAKLVANHIGSVHHEINFTVQEGLDAISDVIYHLETYDVTTVR
                     ASTPMYLLARVIKSMGIKMVLSGEGADELFGGYLYFHKAPNAQAFHEETVRKLSKLHL
                     YDCLRANKSLAAWGIEGRVPFLDKEFMDVAMQLNPADKMSGPDKMEKWILRKAFEDYL
                     PKEIAWRQKEQFSDGVGYSWIDTLKEVAAKTVSDDELANAKFRFPVNPPMSKEEYRYR
                     CIFTEHFLSDQAAACVPSVKSVACSTPEALAWDASFQNLNDPSGRAVKSVHNQSY"
BASE COUNT          497 a          311 c          353 g          461 t
ORIGIN      
        1 caagattgcg tcttttgaat gcaccgacct gccgttgatc cgcaaagtgg cggccaaccc
       61 ttatttaatt ctgaaaaaac attggctctt gctgtaaatg gcgaaattta taatcacatg
      121 gatttgcgta agcaactgcc cgattatcag tttttaaccc acagcgactg cgaggtaatt
      181 ttagcgctct atcaacaaaa aggtgtaaat tttatcgaag accttaacgg catttttgcc
      241 ttcgctttat acgatacgca aaacgacagc tatcttattg cacgcgatca catgggcatt
      301 attccgcttt atatgggttg ggataagcat ggcaattttt atgtatcgtc tgaactgaaa
      361 gctttagaag gggtatgtaa taaaattcag gaatttttgc ccggccacta ttggcatagt
      421 aaagaaggca cattaaaaaa atggtatcaa cgtaactgga cgcagtatga gaatgtgaaa
      481 gataataaaa gcgatataaa tcaattacgc acagcgttag aagcagcggt acatcgtcag
      541 ctaatgagcg atgtacccta cggagtgttg ctttcgggcg gattagattc gtcaatcata
      601 tcagcaatta ccaaaaaata tgcacctaac cgtatcgaaa gcaacgatac acaaatggca
      661 tggtatcctc aattgcatag ctttgctgtg ggtttaaaag gctcgcccga tttggctgca
      721 gcaaaattgg ttgccaacca tattggcagt gttcatcacg aaattaattt caccgtacaa
      781 gaaggcttag atgcaattag cgatgttatc tatcatctcg aaacgtatga tgttactacc
      841 gtgcgtgctt caacacctat gtatttattg gcgcgtgtaa taaaatcgat gggtattaaa
      901 atggttttat cgggcgaagg tgctgacgag ctttttggcg gctatcttta ttttcacaaa
      961 gcacccaatg cacaggcttt tcatgaggaa acagttcgaa aattaagtaa gctgcattta
     1021 tatgattgtt tgcgcgctaa taaatcatta gcggcatggg gcattgaagg acgtgtgcct
     1081 tttttagaca aggagtttat ggacgtggcc atgcaattaa atccggccga taaaatgtcg
     1141 gggccggaca aaatggaaaa atggattttg cgtaaagctt tcgaagatta tttaccaaaa
     1201 gaaattgcct ggcgccaaaa agaacagttt agcgatggtg tgggttatag ctggatagat
     1261 actttaaaag aagttgccgc aaaaaccgtt tctgatgacg aattggccaa tgctaaattc
     1321 cggtttccgg taaacccacc aatgagcaaa gaagaatatc gttaccgatg tatttttacc
     1381 gaacactttc tgagcgatca ggctgcagct tgtgtaccat cggtaaaatc ggtggcatgc
     1441 agtacacccg aagcgttggc ttgggatgca tcattccaaa acctaaacga cccatcaggt
     1501 cgtgcagtta aaagtgtgca caatcaaagt tattagtcct atatagtaac tgtaacttat
     1561 tgtttctaat gtagcaatta caatatgcaa cccggctaca atcatttgtt atcaatttta
     1621 ta
//
LOCUS       DAOMAA010000540         1620 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_84991_length_1620_cov_2.959105, whole genome shotgun sequence.
ACCESSION   DAOMAA010000540 DAOMAA010000000
VERSION     DAOMAA010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84991_length_1620_cov_2.959105"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="PLO59_07445"
     CDS             <1..584
                     /locus_tag="PLO59_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009630369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HQV00978.1"
                     /translation="LAIRNESGHELLDAGASKVFAVADHQVAHIYLNDKSIFNEVLAL
                     LKSNQAIEHVWHGNELLQQGLQHKRCGDMVCVAKPHAWFTYYYWHNDNLAPDFAPTVD
                     IHRKPGYDPAELFINPKIKLPILKAGYTLLKKKLGFRYLMDVIPMHAEMVKGSHGAVP
                     QSKTQWPIVISNTQKKEVLATDVFDIIKQTITG"
     gene            622..1020
                     /gene="rnpB"
                     /locus_tag="PLO59_07450"
     ncRNA           622..1020
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLO59_07450"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            1264..1503
                     /locus_tag="PLO59_07455"
     CDS             1264..1503
                     /locus_tag="PLO59_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00979.1"
                     /translation="MKTYTLNTRSLNVFIALLLLICLRPQNDYAQTTATVAITTSRTL
                     PQYFCGDNGTNTIQDNQGYNCFNNVSQTYDQLLMI"
BASE COUNT          546 a          303 c          311 g          460 t
ORIGIN      
        1 ggctggctat acgcaatgaa agcggacacg agctgttaga tgcaggcgct tcaaaagttt
       61 ttgcagtggc cgaccatcag gtggcacaca tctatctgaa cgataaaagt atttttaatg
      121 aagtgctggc tttgctaaaa tcaaaccaag ctattgaaca tgtttggcat ggtaatgaat
      181 tgctacaaca agggttacaa cataagcgtt gtggtgatat ggtatgtgtg gcaaaaccac
      241 atgcctggtt tacctattat tattggcata acgataacct tgcccccgat tttgcaccta
      301 ctgttgatat acaccgcaaa ccgggctacg acccagccga actttttata aatccaaaaa
      361 ttaagttacc tatacttaaa gcaggctata ccttattgaa aaaaaaatta ggcttcaggt
      421 acttaatgga tgtaataccc atgcatgccg aaatggtaaa aggatcgcat ggggccgtac
      481 cgcaatcaaa aacacaatgg cctatcgtta tttctaacac acaaaaaaaa gaagtgttgg
      541 ctaccgatgt atttgatatt ataaaacaaa caataaccgg ataagcccaa caaataacaa
      601 gggtttgtta tatttgcaca gtcggcaaaa ggcttcatcg ctgcatgtaa taaggcagag
      661 gaaagtccgg gcaacacaga gcgccatact tcctaacggg aagggtttta agaaattaaa
      721 atacagcaag tgccacagaa aataaccgtt tcgcagcaat gcggaataag ggtgcaaacg
      781 tgaggtaaga gctcacgcag cccgcagtaa tgttgggttg gggtaaacct tatgggttga
      841 aaggccacat aggttgtaag ttataaacgg ctcgtttatg caacatgtgc aaacatgttg
      901 ccttacaatg ggtaggctgc tacaggttgt acgcgagtac aatcgcagat aaatgatgaa
      961 ggacttatta ctgccagcag ttataagtaa cagaacccgg cttattattt tgccgacacc
     1021 aaataaaacc tgtatgagca ttggcttgtg caggtttttt taatatctgt aattttattt
     1081 cgaagcatca aacaaactgt tttttaaatt ataactgata ttaaaaaaat taaaaaacag
     1141 gcaacaaaaa attaatacag gcaaaacaat tattaaacaa attttttatt tttgattaaa
     1201 gcctttgcct tataatgatt gttgttgcta ttaatcgttt cataaaatta aattttaaat
     1261 gctatgaaaa catatacact aaatacccgc tctttaaatg tatttatcgc tttacttctg
     1321 ttaatatgtt tgaggccaca aaatgactac gcccaaacta ctgcaactgt tgccataaca
     1381 acttcgcgca cgctaccaca atatttttgt ggcgataatg gcaccaatac cattcaggat
     1441 aaccagggat acaattgttt taataatgta tctcaaactt acgaccaatt attaatgatt
     1501 taggtatggc taacctacgg tatccgggcg gtacggtatc aaatttttac gactggcgca
     1561 aaggttattt tctcaataag ttacctgaca gttttattat gccaaacaat tttgaagcat
//
LOCUS       DAOMAA010000541         1620 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_85039_length_1620_cov_2.276677, whole genome shotgun sequence.
ACCESSION   DAOMAA010000541 DAOMAA010000000
VERSION     DAOMAA010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85039_length_1620_cov_2.276677"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="PLO59_07460"
     CDS             complement(<1..798)
                     /locus_tag="PLO59_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HQV00980.1"
                     /translation="MRELAFREALREAMSEEMRRDAQVFLMGEEVAQYNGAYKVSQGM
                     LDEFGPKRVIDTPIAELGFAGIGVGASMNGLRPIIEFMTFNFSLVAIDQVINSAAKMM
                     SMSGGQFTCPIVFRGPTASAGMLSSQHSQAFENWYANCPGLKVIVPSNPADAKGLLKS
                     AIRDNDPVIFMESEQMYGDKGLVPDGEYLMPIGVADIKRTGTDVTLVTFGKMLKVVMA
                     AADELAKDNINAEVIDLRTVRPIDYATVISSVKKTNRLVIIEEQWPFA"
     gene            complement(868..>1620)
                     /locus_tag="PLO59_07465"
     CDS             complement(868..>1620)
                     /locus_tag="PLO59_07465"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV00981.1"
                     /translation="TDTSDCSPVAHINTASIVSTCDTTYAFSTPYHPNLVYQWNLNGN
                     AIAGADSAYYVAIQSGTYSVTVANFINACNNTSDTVQLTLGIIVQPTITGLDTLYCSN
                     AATVTLSGFPAGGTFSGNGINGNTFNAQTAGVGSHTITYQYQLNGCNASTQLSTTVST
                     CAGINENQSANLTVIPNPNNGSFNIRFNQNQTETITVRVVDASGKTIYKQQHQVNAGT
                     VNLPLQIQTKAGVYTLQIQNKAGKQQLPFIIE"
BASE COUNT          424 a          332 c          350 g          514 t
ORIGIN      
        1 agcaaatggc cactgctctt caataatcac taaacgattg gtttttttaa cgctgctgat
       61 tacagttgcg taatcaatag ggcgcacggt acgtaaatca attacctcgg cattgatatt
      121 atcttttgcc aattcatcgg ctgcagccat tactacttta agcattttac caaaggttac
      181 taacgttaca tcggtgccgg ttcgtttaat atcggcaacg ccaatgggca tcaggtattc
      241 gccatcgggt actaaacctt tatcaccgta catctgttca ctttccataa aaataacggg
      301 gtcgttatcg cgtatggcgc tttttaataa tcctttggca tcggccggat tgctgggcac
      361 aattactttt aagccggggc agttggcata ccagttttca aaggcttgcg agtgttgtga
      421 gcttagcata cctgcgctgg ctgttggccc tctgaatacg atagggcatg taaactggcc
      481 gccactcata ctcatcattt ttgcagcgct gtttattacc tggtcaatag caaccagcga
      541 aaaattaaat gtcataaact caattatagg ccgtaaacca ttcatgctgg cacctacgcc
      601 tataccggca aaacctaatt cggcaatggg tgtatcaata acacgctttg gcccaaactc
      661 gtctaacatg ccttggctta ctttatatgc gccattgtat tgggctactt cttcgcccat
      721 taaaaatacc tgggcatctc tgcgcatttc ttctgacatg gcctcgcgta aggcttctct
      781 aaatgctaat tctctcattg tacgtgattt ttattggggg gtgaaaatac atttttaaca
      841 aagccgtaaa aatgtaattg tttgcagcta ttcaattata aatggcaact gttgtttgcc
      901 ggctttattt tgtatttgca gtgtataaac accggctttt gtttgaattt gcaagggcag
      961 gtttactgta cctgcattaa cctgatgctg ttgtttgtaa atggttttgc ctgatgcatc
     1021 gacaacccta acggttatag tttcggtttg attttgattg aagcgaatat taaaacttcc
     1081 gttgttggga ttgggtataa cggtaaggtt agcagattgg ttttcattga tacctgcgca
     1141 agtagatacg gtagtagata attgtgtgct ggcattacag ccattaagtt ggtattgata
     1201 ggtaatagtg tggctgccaa cacctgcagt ttgtgcatta aaagtattgc catttatacc
     1261 gttgccacta aaagtaccac ctgccggaaa tccgctgagt gtaacagtgg ccgcattgct
     1321 gcaatagagc gtatctaacc ctgttatggt tggctgaaca attataccca aagtaagctg
     1381 tactgtgtcg gaagtattgt tacatgcatt aataaaattg gcaactgtta cgctgtatgt
     1441 accgctttgt atggctacat aatatgcact atcggcaccg gcaatagcat tgccgtttaa
     1501 attccattga tatactaaat taggatggta aggtgtgcta aatgcatacg tagtatcgca
     1561 agttgataca atggaggccg tatttatatg tgccaccggg ctgcaatcgg aagtatctgt
//
LOCUS       DAOMAA010000542         1619 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_85124_length_1619_cov_2.778133, whole genome shotgun sequence.
ACCESSION   DAOMAA010000542 DAOMAA010000000
VERSION     DAOMAA010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85124_length_1619_cov_2.778133"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="PLO59_07470"
     CDS             <1..278
                     /locus_tag="PLO59_07470"
                     /inference="COORDINATES: protein motif:HMM:NF026044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HQV00982.1"
                     /translation="KLIQKLKKLRVKALPAKLPDTIDINIEALKIGDSFKVGQLALDG
                     LTILDAANNVIVGIRMTRNVVSAEAAAKAAAPAAKAAPAAKAAPAKK"
     gene            300..866
                     /gene="pth"
                     /locus_tag="PLO59_07475"
     CDS             300..866
                     /gene="pth"
                     /locus_tag="PLO59_07475"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HQV00983.1"
                     /translation="MKFLIAGLGNIGAEYDNTRHNIGFEVLNTFARSEGVFFSTQRLA
                     MVTEVKFKGRTLLLVKPTTYMNLSGKAINYWMQAEKITKQNLLVITDDIALPFGALRM
                     RGSGSDGGHNGLKDIQQTLATTDYARLRFGVGNNFARGRQVEYVLGAWDAEQANELPI
                     KINRAIDAIKSFVAIGISQTMTQYNKNS"
     gene            976..>1619
                     /locus_tag="PLO59_07480"
     CDS             976..>1619
                     /locus_tag="PLO59_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV00984.1"
                     /translation="MKKILYVCLAFIITNYAHAQSIVLQENFTTYDSTTASIPAGWIL
                     SYTGNGSYYTSTTSSGPSGPNSYKFGVDSATVTTPAFTNADSVHFWMRGNGTDSLSTL
                     TLNYTTDLISWNLLTVIYPVNNVSATKHYALPAGTKQLQFMYSKSLGNVAFDDFSVTA
                     NVSGIYKTEKVPYSIYPMPAKNVFYTAFNEPQKNTTVTLYNVLGRPIKTQADWI"
BASE COUNT          491 a          343 c          323 g          462 t
ORIGIN      
        1 gtaagcttat acaaaaactt aaaaaattac gcgtaaaagc attacctgct aaattacccg
       61 ataccattga cattaatatc gaagccttaa aaattggcga ttcgtttaaa gttggtcaat
      121 tggcattaga tggtttaacc attttagatg cagccaataa cgttattgtg ggtattcgta
      181 tgacgcgtaa tgtagtaagt gccgaagccg ctgctaaagc tgccgcacct gctgccaaag
      241 ctgcaccggc cgctaaagct gctccggcta aaaaataatt aaactaaaaa ccgcgtctga
      301 tgaaattttt aattgccggc ttgggtaaca tcggtgccga atacgacaat accagacata
      361 acataggttt tgaggtatta aacaccttcg cacgaagcga aggtgttttt ttttcgaccc
      421 aacgtttggc catggtaacc gaagttaaat ttaagggacg taccttattg ctggttaagc
      481 cgaccactta tatgaattta agcggtaaag ccattaacta ttggatgcaa gccgaaaaaa
      541 ttaccaagca aaatctttta gttattaccg atgacatagc cttgcctttt ggtgcattgc
      601 gtatgcgcgg cagcggtagc gatggcggcc acaatggcct taaggacatt caacaaacct
      661 tagccacaac cgattatgcc cgcctaaggt ttggtgtggg caataatttt gcccgtggcc
      721 gccaggtgga gtatgtattg ggcgcatggg atgccgagca agccaatgaa ttgcccatta
      781 aaattaaccg cgctattgat gccattaaaa gttttgtggc tattggtatt tcccaaacca
      841 tgacgcaata caataaaaac agttagttgt aaaatttgcc gttgtagtat gtactttaac
      901 tattaacctg ctattaacag cattaacaat aactacgctt atgtttgttt cgtcaatagc
      961 tctaaaaata gtactatgaa aaagatttta tacgtctgtc tggcttttat aattaccaat
     1021 tatgcgcatg cgcaaagtat cgttttacag gaaaacttta ccacttacga tagcactact
     1081 gccagtattc cggccggttg gatattaagc tataccggta atggttctta ctatacttca
     1141 accaccagct cggggccaag tggtcccaac tcctataagt ttggtgttga tagcgctacg
     1201 gttaccacac ctgcatttac aaatgccgac agtgtacatt tttggatgcg cggcaatggc
     1261 accgacagcc tgagcacact tacactcaac tacacaaccg atttgataag ctggaattta
     1321 ctaaccgtaa tttatccggt taataatgtt tcagctacca aacattatgc tttgcctgcc
     1381 ggcaccaaac agttgcagtt tatgtacagc aaatcgttag gtaatgttgc tttcgatgat
     1441 ttttcagtta cagcaaatgt ttcaggtata tataaaaccg aaaaggtgcc ttattcaatt
     1501 taccctatgc cggccaaaaa tgtgttttac acagcattta acgaaccaca aaaaaacaca
     1561 acggtaactt tatataatgt actgggtcgc cccattaaaa cgcaggccga ttggattag
//
LOCUS       DAOMAA010000543         1619 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_85172_length_1619_cov_1.955243, whole genome shotgun sequence.
ACCESSION   DAOMAA010000543 DAOMAA010000000
VERSION     DAOMAA010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85172_length_1619_cov_1.955243"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="PLO59_07485"
     CDS             complement(<1..707)
                     /locus_tag="PLO59_07485"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQV00985.1"
                     /translation="MLLRLFIKNYALINQLDISFEPTFVIITGETGAGKSILLGALNL
                     ILGSRSDTTMLLDQKQKCIVEAEFDISAYNLQWLFTQFELDYQPHTIIRREINAEGKS
                     RAFINDTPINLTVLRDLAIQLVDIHSQRDSLALNDANYQMQVIDAFAKNRDLLLQYSQ
                     LHKTYNKAVTNLHALIEQDKTAKANFDFIQFQWQELQVLQLHADEQETLEHELEKLEH
                     ADAIMQHLSMALNILQQ"
     gene            complement(794..>1619)
                     /locus_tag="PLO59_07490"
     CDS             complement(794..>1619)
                     /locus_tag="PLO59_07490"
                     /inference="COORDINATES: protein motif:HMM:NF027445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HQV00986.1"
                     /translation="QVNILTPNIQGTSNTVFDAMRTQVRDLMNNTRWTNDKYQNEERI
                     ECQITITITEKISTTDFKGKIEVKSSRPIFKSTYNSPMLAISDQQFVIKFDEFQILEY
                     NETNVNQNLVSILAYYAYIIVGFDYDSYSLLGGDMYFTKAQAIVNQMTQAPQPGWRPF
                     DSDRNRYWLAENLVAPVYRPIRNLVYNMHLKAMDNMTKDIDNGVRTIADGLDLMIPVH
                     KDKPNAYLLSVFFDAKSDELVNIFKSGPSDVKARAKQTLELINPGNSSKYAKILEN"
BASE COUNT          471 a          298 c          296 g          554 t
ORIGIN      
        1 tcctgttgca gtatatttaa agccattgat aaatgctgca tgatagcgtc agcatgttct
       61 aatttttcta attcatgctc taacgtttct tgttcatcgg catgtaattg caacacttgt
      121 aactcttgcc attgaaattg tataaaatca aaatttgcct tagccgtttt atcttgttct
      181 attagtgcat gtaggttggt aacggccttg ttatacgttt tatgcaattg gctatactgt
      241 aacagtaaat ctctgttttt ggcaaatgca tcaatcacct gcatttgata gtttgcatca
      301 tttaaagcca aactatcacg ttgcgaatgg atatccacta attgaattgc caagtcgcgt
      361 agtaccgtta aatttattgg tgtgtcgttt ataaaagctc gcgatttgcc ttcggcattt
      421 atttcacgtc ttattattgt atggggttgg taatcgagtt cgaattgtgt aaacaaccat
      481 tgcaggttat atgcactaat atcaaattcg gcctcaacaa tacacttttg tttttgatcg
      541 agcagcatag ttgtatcgga acggctaccc aaaattaaat tcagtgctcc taataaaata
      601 gacttacctg caccggtttc accggttata attacaaagg ttggctcaaa actaatgtct
      661 aattgattaa tgagcgcgta atttttaata aacaaacgta aaagcatact ggcgaatcta
      721 aaaaaaatgc ttgctcacta cttttataaa acgaaattgt tttgagctac tatcctattg
      781 aaaacagctt tggttagttt tcaagtatct tggcatactt gctcgaattt cccggattta
      841 ttaattcaag agtttgttta gctctggctt taacatcgct agggcccgac ttaaatatat
      901 taaccagttc atcactcttg gcatcaaaaa acacacttaa taaatatgca ttgggtttat
      961 ctttgtgaac gggtatcatt aaatctaaac catcggcaat agtgcgcaca ccgttatcaa
     1021 tatctttagt catgttgtcc attgccttca ggtgcatgtt atacaccaaa tttcgaatgg
     1081 gccgataaac aggcgcaacc aaattttcgg caagccaata acggttacgg tcgctgtcaa
     1141 atggccgcca accgggctgc ggtgcttgtg tcatttgatt tacaatggct tgtgctttgg
     1201 taaaatacat atcgcctcca agtaaactgt aggaatcgta atcaaagcct actataatat
     1261 atgcatagta agcaagtatg cttacaaggt tttgattcac attggtttcg ttatattcta
     1321 aaatttgaaa ctcgtcaaat tttatcacaa attgttggtc ggatatggcc aacatgggtg
     1381 agttataagt tgacttaaaa ataggacgcg atgatttaac ttcaatttta cctttgaaat
     1441 cagtagttga aattttttca gtaatcgtaa tggttatctg acattcgata cgctcttcgt
     1501 tttggtattt atcattggtc cagcgggtat tattcatcaa atcgcgtacc tgtgtgcgca
     1561 tggcatcaaa aaccgtatta ctggtacctt gaatattggg cgttaaaata ttaacctgg
//
LOCUS       DAOMAA010000544         1615 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_85510_length_1615_cov_3.887821, whole genome shotgun sequence.
ACCESSION   DAOMAA010000544 DAOMAA010000000
VERSION     DAOMAA010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85510_length_1615_cov_3.887821"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="PLO59_07495"
     CDS             <1..364
                     /locus_tag="PLO59_07495"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HQV00987.1"
                     /translation="GVKKYLKYCQVEEEATNRPYSSRYIGSLVSDFHRNMIKGGVYIY
                     PSTAKSPKGKLRLLYECNPLAFIVEQAGGLASDGFNRILEMQPKSLHQRCPLFIGSYE
                     MVKKAEEFMQKYSTPEVQ"
     gene            404..1198
                     /locus_tag="PLO59_07500"
     CDS             404..1198
                     /locus_tag="PLO59_07500"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQV00988.1"
                     /translation="MKNKWMPVVVVLFVVTTVAIYLLPINKKQEAKPQSHSHTKGDFE
                     SARMSLVNALPKPQQQVLAQLEKAGNNDSLALFWETMQQPGLSAHYFELIANLQKTER
                     SFIEAAYRYYDAFKAEPDSMLRTFYVSKAINNYESVLALNPKNLNAKTDLGLCYAEGS
                     NEPMKGIMLLRDVVTENPKHENAQLNLGFLSLKSNQISKAIERFQTVLEINPKRTDVY
                     IYLTQAYLQQGDSAEAGRQLHKFIDQTTDNKAKEQAKGYLQELGLP"
     gene            1348..>1615
                     /gene="rplM"
                     /locus_tag="PLO59_07505"
     CDS             1348..>1615
                     /gene="rplM"
                     /locus_tag="PLO59_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013070027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HQV00989.1"
                     /translation="MDTLSYKTVSANKATVNKKWLIIDAENLVLGRLSTAVASRLRGK
                     HKADFTPHADCGDNIIVINTAKVKLTGRKMSEKQYVRHTGYPGGQ"
BASE COUNT          558 a          319 c          317 g          421 t
ORIGIN      
        1 tggcgtaaaa aaatatttaa aatattgtca ggtagaagaa gaggctacca accgcccata
       61 ctcatcacgt tacattggct ctttggtaag cgatttccac cgcaacatga taaagggtgg
      121 cgtttatatt tatccgtcaa cggcaaaatc gcctaaaggc aaacttcgat tactttatga
      181 gtgcaatcct ttggcattta tagttgaaca agccggtggt ttagcttctg atgggtttaa
      241 ccgcatactg gaaatgcaac ccaaatcatt acatcagcgt tgtcctttat ttattggatc
      301 ttatgaaatg gtaaaaaaag cagaagagtt tatgcaaaaa tattccacac cggaagtgca
      361 gtaaataagt aaccgttaag taccaatacc tgagtttttg taaatgaaaa ataaatggat
      421 gcctgttgtg gtggttttgt tcgtagttac gacagttgcc atatacttat taccaataaa
      481 taaaaaacaa gaggctaagc cacaaagcca ctcacatacc aaaggcgatt tcgaaagtgc
      541 ccgcatgtca ttagtaaatg cactacctaa gccgcaacaa caagttttag cacaattaga
      601 gaaggcaggc aataacgact cattggcttt attttgggaa accatgcaac agccgggctt
      661 atcagcacac tacttcgaat taattgccaa tcttcaaaaa accgaacgca gctttatcga
      721 agccgcatac agatactatg atgcttttaa agctgaaccc gactcaatgc tacgcacatt
      781 ttatgttagc aaagcaataa acaattacga atcggtttta gcccttaacc ccaaaaacct
      841 gaatgcgaaa accgatttag gattatgtta tgccgaaggt tcaaacgagc ccatgaaagg
      901 cattatgttg ctacgtgatg tggttaccga aaatccgaaa catgaaaacg cacagctcaa
      961 tcttggtttt ctttcattaa aatcaaatca gataagcaaa gccatcgaaa gatttcaaac
     1021 ggtgttagaa ataaatccca aacgtaccga tgtttatatc tatttaacgc aagcatactt
     1081 gcagcaaggc gattcggctg aggccggccg gcagctgcac aagtttattg accaaacaac
     1141 agataacaaa gccaaagagc aggcaaaagg ctatttgcaa gagttgggat tgccgtaaat
     1201 actagcaaaa gagcgcatct taaagttgag atttaccaac tttaagcttt gtaataattg
     1261 gctgaaaaca atttgagtaa tgtatttgcg catttaaaaa ttgtgtttac ttttgcgacc
     1321 cttaaaaaaa ataaatatta acaagtagtg gatacattat catacaaaac agtatcagca
     1381 aacaaagcca ccgtaaataa gaagtggttg attatagatg cagaaaacct ggtattaggc
     1441 cgcctgagta ctgctgttgc ttcacgctta cgaggcaaac acaaggctga ctttacacca
     1501 catgccgatt gtggcgacaa tataattgta ataaatacgg ctaaggtaaa acttacaggt
     1561 cgcaagatgt cagaaaagca atatgtgcgt cataccggtt atccgggcgg gcagc
//
LOCUS       DAOMAA010000545         1614 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_85651_length_1614_cov_2.772290, whole genome shotgun sequence.
ACCESSION   DAOMAA010000545 DAOMAA010000000
VERSION     DAOMAA010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85651_length_1614_cov_2.772290"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..1271
                     /locus_tag="PLO59_07510"
     CDS             156..1271
                     /locus_tag="PLO59_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00990.1"
                     /translation="MSAALKIILTVSIFLFPFIAYSQPVASDNCASAPLINTLGTYTG
                     NTGNFTPNDALWFKNSNNFCSSVAKQTVENNGFYKFIASSTSISYTVCAPTGCVNHNS
                     FFGISGIQFLIFEMPSGICGVGSITKKHCIPQLSGTDCGSCGNPKGCLSFTTGGFSIG
                     TTYYVMIDGYEGDICPYSIQFTTGIVLPVELISFYGTPADNGNKLQWTTATEINNNHF
                     DVECTPDGKNFKTIGTLKGMGNSSVPVTYNFLDERPADFITYYRLKQTDHNGEVHYSH
                     IINVKNNVVQHAQLTPNPAIDELTVETIVQSNGLYLFSIEGITGNCWQEEFYLTKGMN
                     QVKVNLTDKLSAGFYIFKVYSLSGHVLSIDKFIKAGN"
BASE COUNT          523 a          297 c          286 g          508 t
ORIGIN      
        1 taattgataa agaaattaaa gaaattagct ttcaaaagaa gcttaaactt taatgccata
       61 taaaccgcgc accgtagata agaaatagta ctatattcgt ttttaataaa accattatgt
      121 tataaataat tacattctta caacatgcga ggtatatgag tgcagccctt aaaattatac
      181 ttactgtttc gattttttta tttcccttta tcgcttattc gcaacctgtt gcatctgata
      241 attgtgcatc tgcgccacta attaatacct taggcacata caccggaaac accggcaact
      301 ttacgcccaa tgatgccttg tggtttaaaa acagtaacaa cttttgttcg tcagttgcaa
      361 aacaaactgt tgaaaataac gggttctata aatttattgc cagcagtact tccatttcat
      421 acacagtttg cgcccctaca ggatgtgtaa atcacaatag cttttttgga ataagcggca
      481 ttcagttttt aattttcgaa atgcccagtg gtatctgtgg tgttggctcc attaccaaaa
      541 agcattgcat acctcagtta agtggaaccg attgtggttc ctgcggtaat ccgaaaggtt
      601 gcctgtcttt tacaacaggt gggttcagta taggcacaac atattatgta atgatagatg
      661 gttatgaggg cgatatatgc ccatactcaa ttcaatttac caccggtatt gtattaccgg
      721 ttgaattaat ttcgttttac ggaacacctg ctgataatgg caacaaactg caatggacaa
      781 cagcaacaga aataaataac aatcactttg atgttgaatg tacacccgat ggaaaaaatt
      841 ttaaaaccat tgggacacta aaaggcatgg gcaattcaag tgtgccggta acttataatt
      901 ttttagatga aaggcctgct gattttatta cctattacag gctaaaacaa accgaccaca
      961 atggcgaggt acactattcg cacattatta atgtaaagaa caacgttgtt caacatgcac
     1021 aacttacgcc aaacccggca attgatgaat taacggttga aacaattgtg cagtcaaacg
     1081 gtctatatct tttttctata gaaggaatta ccggaaattg ttggcaggaa gaattttacc
     1141 tgaccaaagg catgaatcaa gttaaagtaa atcttactga taagttaagt gccggctttt
     1201 atatttttaa agtttattcc ttatcgggac atgtcctgag catagataaa tttattaagg
     1261 caggtaatta gccttttacc ggacaagagt ttcacatggt ttccggtaca ttttaatttg
     1321 tgttgtattc ttactaagct tcaattgttt aatggaatta aacaattgtt atatcaattt
     1381 cactctacat tgatttactt acctaagtcc ggaagaaaca ttttgtaacc taaccgtttt
     1441 tcatttcctc taatagaata taactcataa aattacttta atatgaaaaa gattctatta
     1501 gcaatggtgc tgcttaccac cacatttgta agcaacaatt caatggcttg cgatgcctgc
     1561 ggcaaaggtg acccggctgt agagctaaga ggagccatgc aaaaattgtg ggcc
//
LOCUS       DAOMAA010000546         1612 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_85852_length_1612_cov_2.999358, whole genome shotgun sequence.
ACCESSION   DAOMAA010000546 DAOMAA010000000
VERSION     DAOMAA010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85852_length_1612_cov_2.999358"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="PLO59_07515"
     CDS             <1..791
                     /locus_tag="PLO59_07515"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HQV00991.1"
                     /translation="TQRIQRPSITYLNPFVNQSDPTNITYGNPELEPEVSHAFEVGYN
                     LFRKFGSINTSVYHRFTDNSIESIRFTDSNNVYNTTYANLGNNTTTGLSVSANIIHKK
                     KLVVGGNFNTFYYTVKSKTANLSNEGLNYSVGIFSSYKFNTQWGVQVFGNFTGPKYSL
                     QGKATSFFFYNASVRREFKNEKGGIGFGLDNFLTPYLKFKNNYSGQDFTFNSTNKFFF
                     LGVRVSFDYRFGKVDFNNKRKKIRNDDLKQGEDGGMGGGMGNSR"
     gene            805..1500
                     /locus_tag="PLO59_07520"
     CDS             805..1500
                     /locus_tag="PLO59_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00992.1"
                     /translation="MKVGLLIGCCITVLIFNVQAQLLDTLTYSWQQPRKPFFALDFSK
                     SFGGKFGADVFGFRGGVVQNKRLYYGLGYYRINSDRVTDIQTVTESGNDTLIPGKLKY
                     RFVSANLEYVFFKTEKFQTSIPANIGFGYSFYEYFKQRGVAQKTTKNHAAIFTMGIAA
                     HYKIVSWIGIGAGTSFASTLGTNPEHQQNFNTLTYNLGVKIFFDDLVAAIKNYKHQQN
                     ENTDDDKPQPDFK"
BASE COUNT          521 a          306 c          308 g          477 t
ORIGIN      
        1 acacacagcg catacaacga cccagcatta cctacttaaa tccatttgta aatcaaagcg
       61 acccaaccaa catcacctac ggtaaccccg agcttgaacc ggaagtgagt catgcttttg
      121 aagtaggata taatttgttt cgcaagttcg gctcaattaa cacatcggta tatcaccggt
      181 ttactgataa ttcaattgaa agcatacgct ttaccgattc gaacaatgtt tacaatacta
      241 cttatgcaaa cttaggcaac aacactacta cgggtttaag tgtaagtgct aatattatac
      301 ataagaaaaa attagttgtt ggcggtaact ttaatacctt ttactatacc gttaaaagca
      361 aaacggccaa cctcagtaat gaagggctta actacagcgt tggtattttt agcagctata
      421 aatttaatac gcaatggggt gtacaggtat tcggaaattt taccggtcct aaatattcgc
      481 ttcagggtaa ggctaccagt tttttctttt acaatgcatc ggtaaggcgc gagtttaaaa
      541 acgaaaaggg cggtattggt tttggtttag ataatttttt aaccccttac ttgaagttta
      601 aaaacaatta tagcggccag gattttacct ttaacagtac caataaattc tttttcttgg
      661 gcgttcgtgt tagttttgat tatcgctttg gcaaggttga ttttaataac aaacgcaaaa
      721 aaatacgtaa tgacgattta aaacaaggcg aagacggagg catgggtgga ggcatgggta
      781 attcgcgcta atttgaatgg gtaaatgaaa gttggtttat taattggatg ctgcataacc
      841 gtactcattt ttaatgtaca ggcacaattg ttagacacgc ttacttatag ttggcagcag
      901 ccgcgcaaac ctttttttgc gttagatttc agcaaatcgt ttggtggtaa atttggtgcc
      961 gatgtgtttg gttttagagg cggtgtagta caaaataaaa gactatacta tggtttgggg
     1021 tattaccgca ttaacagcga cagggttact gatatacaaa ccgttaccga aagtggtaac
     1081 gacaccctta tacccggtaa actaaaatat cgttttgttt cagccaactt agaatatgtt
     1141 ttttttaaga cagaaaaatt tcaaaccagc atacctgcta acattggttt cggctatagt
     1201 ttttacgagt actttaaaca gcgcggtgta gcacaaaaaa ccacaaaaaa ccatgcggca
     1261 atttttacta tgggtattgc cgcacattat aaaattgtaa gttggattgg cattggtgca
     1321 ggcacttcat ttgcatcaac tttaggaacc aaccctgaac atcaacaaaa ttttaataca
     1381 cttacgtaca atttaggtgt gaaaatattt tttgacgact tggtagctgc aattaaaaac
     1441 tacaaacacc agcaaaacga aaacaccgat gatgataagc cacaacccga ttttaaataa
     1501 ccacacacat gcctacagat attagagttg atgcttttat taaaaaagca gccccgtttg
     1561 cacaacccat tttggaatac ctacgcctat gcgtacacga agcctgcccc ga
//
LOCUS       DAOMAA010000547         1610 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_86130_length_1610_cov_2.224437, whole genome shotgun sequence.
ACCESSION   DAOMAA010000547 DAOMAA010000000
VERSION     DAOMAA010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86130_length_1610_cov_2.224437"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="PLO59_07525"
     CDS             complement(<1..442)
                     /locus_tag="PLO59_07525"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HQV00993.1"
                     /translation="MHKLLSSILISCLVLLTISDLKAQNKFTISGYVKDASTGETMIG
                     ANVYLKETQKGTTTNVYGFYSFTVESGTYTLVATYLGYVEEEQKVNLNQNLQVAIAIK
                     PKVVETQAAEVVGSKVENKISSTQMGKEVLEIETIKSIPAFLGEV"
     gene            complement(531..>1610)
                     /gene="pepD"
                     /locus_tag="PLO59_07530"
     CDS             complement(531..>1610)
                     /gene="pepD"
                     /locus_tag="PLO59_07530"
                     /EC_number="3.4.13.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-Ala-His dipeptidase"
                     /protein_id="HQV00994.1"
                     /translation="LLAATDATHPPIEALFTIDEETGMTGAKGLQPGWLKGKLMLNLD
                     TEEDDEIDIGCAGGIDITVNATYTPEKAADNYLSYIVTVTGLNGGHSGLEIHKGLGNA
                     NKIMNRILYHVQSLGLRINSVDGGSLRNAIPRESKAVISIPDTNSQAFENSINQISQI
                     IKSELACTDAGLIITALKTNPADNWMPQQLQLSFLKCIYAAQNGVYAMSHVVPNLVET
                     SNNLACVAAGNGQIKILCLTRSASETAKTDLAQSIQSAFELMPAEVTLSGSYPGWQPD
                     ANSKLLKHVTAIYTQLNKQQPKIVACHAGLECGIIKSKYPLMDMISIGPIIKGAHSPN
                     ERVHIKSVQKFWVFLNEILKTIPEV"
BASE COUNT          453 a          304 c          291 g          562 t
ORIGIN      
        1 caacctcgcc taaaaaagca ggtattgatt taatggtttc tatctctaac acttccttgc
       61 ccatttgtgt tgagcttatt ttattttcga ctttacttcc aacaacttca gcggcttgtg
      121 tttctaccac ttttggtttt atggcaatag ccacttgtaa attttgattt aggtttactt
      181 tttgttcttc ttctacatat cctaaataag tggctactaa tgtgtaggtg ccactttcaa
      241 cggtaaacga ataaaaaccg taaacgttgg tggtggttcc tttttgcgtt tcttttaaat
      301 atacattggc tccaatcatg gtttcaccgg tagatgcatc ctttacgtaa ccactaattg
      361 taaatttatt ttgtgctttt aaatcgctta tggtaagcag taccagacag gatataagaa
      421 ttgaacttaa tagtttgtgc ataaaaggtg aaatagggag gctgaatagt tggcgtttta
      481 gaagctataa cttaccgttt gttacactta gaataaaaac aaatgtcata ttaaacttct
      541 ggtattgttt tgagaatttc gtttaaaaac acccagaatt tttgcaccga tttaatatgt
      601 acacgctcat tgggcgaatg tgctccttta attattgggc ctatcgaaat catatccatc
      661 aacgggtatt tgctttttat tataccacac tctaaacctg catggcaggc aacaatttta
      721 ggttgctgtt tatttaattg cgtataaatt gctgttacat gctttaatag ttttgaattt
      781 gcatcgggtt gccaacccgg atagctgccc gaaagtgtaa cttcggctgg cattaactca
      841 aatgctgatt gaatagattg agctaagtct gttttagctg tttcggatgc agagcgtgtt
      901 aaacacaata ttttaatttg gccattacca gcagcaacac aggcaaggtt atttgaggtt
      961 tcaaccaaat ttggtacaac atggctcatt gcataaacac cattttgtgc cgcataaata
     1021 cattttaaaa aagatagctg aagttgctgt ggcatccaat tatcggccgg attggtttta
     1081 agagcagtaa taattaagcc ggcatcggta catgcaagtt ctgatttaat gatttgtgaa
     1141 atttgattga tgctattttc aaatgcctga ctatttgtat caggaatact tataacagct
     1201 ttgctctcgc gtggaattgc gttacgaagg ctgccaccat caacactatt aatacgtagc
     1261 cccaaggatt gcacatgata caatatgcgg ttcattattt tattggcatt acctaagcct
     1321 ttatgaattt ccaatcctga atgaccaccg tttaaccctg taacagttac tatatatgac
     1381 aaataattat cagccgcttt ctctggtgta tatgtagcgt taactgtaat atcaataccg
     1441 cctgcacaac caatatctat ttcatcgtct tcttcagtat ctaaattcaa catcagtttt
     1501 cctttcaacc aaccgggttg taatcctttt gcacctgtca tgccggtttc ttcatcaatt
     1561 gtaaataaag cttcgatggg tggatgagtt gcatctgttg cggctaataa
//
LOCUS       DAOMAA010000548         1609 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_86237_length_1609_cov_2.430502, whole genome shotgun sequence.
ACCESSION   DAOMAA010000548 DAOMAA010000000
VERSION     DAOMAA010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86237_length_1609_cov_2.430502"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..517)
                     /locus_tag="PLO59_07535"
     CDS             complement(119..517)
                     /locus_tag="PLO59_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV00995.1"
                     /translation="MKSKVGLFLLMLFFAACTDEETLPEGDSRLLYTGDWLVNEQSKL
                     NGNSAYPSTISSIGENDSISIGNFYGLGNSINCVAIVVGNSITIPFQDVDNGILVTGS
                     GLRNTNGSFSITYYTNDSGSRDTVTATYKR"
     gene            complement(568..>1609)
                     /locus_tag="PLO59_07540"
     CDS             complement(568..>1609)
                     /locus_tag="PLO59_07540"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV00996.1"
                     /translation="SDAQQNFDWLCNQYSQLMGLCGSVCAAPLQVNALAQPVYCFGDS
                     TGIISAQTSGGNAPYTYLWSNGCTVSTSSNVVANTYTVTVTDFCGITKTASAAVVQPD
                     ELFITNTITNNTCGINDGAITLQVSNGISPYSFLWNDGNTNQTITNLLDTNYTVTVTD
                     AFDCSKTVQLILGCIPPKPLQIIGPYEICPHQQNVVYSTQPVAGATVYTWSKPGKAEI
                     ISGQGTTSIVVNYGNQGGHVQVKAGNSNGYSLNKKQLIQLGSNCRLAHSGFDAINIYP
                     QPCNGQLIVSGANQVSLLHLTNQLGQNITSIFNNSQLSDILINTAELNNGIYYLKLIS
                     GDGLKVYKVIKN"
BASE COUNT          503 a          315 c          279 g          512 t
ORIGIN      
        1 gatctgtttc gaattatata gaaccgcgaa ataagcataa ctggcagtta caaaaatcaa
       61 ttaaagcatt acaaaaaatt gtaattgagt ggttacaact taacaaatta attacacatc
      121 agcgtttata agttgctgta accgtatctc ggcttccact gtcatttgta taataggtaa
      181 tagaaaaact tccgttagta tttcgtaagc cactacctgt aactaatatg ccattatcaa
      241 catcctgaaa cggaattgta atggagttgc caacaacaat cgccacgcag tttatactat
      301 ttcccaaacc ataaaaatta ccaatgctta tactatcatt ttcaccaatt gaacttatgg
      361 ttgatggata agcactatta ccattaagtt tgctttgctc gtttaccaac caatcaccag
      421 tatataaaag tctgctatct ccttcgggaa gcgtctcttc atccgtacat gccgcaaaaa
      481 ataacataag taaaaacaaa ccaactttac ttttcataat acctaaacgt ttatgcacaa
      541 taatgcgact ttatagtatg tgttcaatta atttttaatg actttatata ctttcagccc
      601 atcgccacta attaatttta aatagtatat gccattgttt aattcggctg tgtttattaa
      661 tatatccgat agctggctat tattaaatat cgaagtaata ttttgtccaa gttgattggt
      721 taagtgaagc aaagaaacct gattggcacc tgaaacaata agttgcccat tacatggctg
      781 tgggtatata ttaattgcat cgaaccctga atgggcaaga cgacaattag aacctaactg
      841 aatcagttgt ttcttattaa ggctataacc attgctgtta ccggctttaa cctgcacatg
      901 cccgccctga ttgccataat ttaccacaat tgaagttgta ccctgaccag aaataatttc
      961 agctttaccc ggcttactcc aagtgtaaac tgtggcacca gctaccggtt gtgtactgta
     1021 aactacattt tgctgatgcg ggcaaatttc gtaggggcct attatttgca gcggttttgg
     1081 agggatgcat cccaaaatta attgtacagt tttggagcaa tcaaatgcat ctgtaactgt
     1141 tactgtatag ttggtatcta acaggttagt gatggtctga ttggtgtttc catcgttcca
     1201 taaaaaacta taaggggata taccattgct gacttgtaag gttatggcac cgtcatttat
     1261 gccacatgta ttatttgtaa tcgtatttgt aataaaaagt tcatcaggct gcaccacagc
     1321 agctgaagct gtttttgtta taccgcaaaa atcagtaaca gttactgtat aggtatttgc
     1381 cactacatta ctacttgtgc tgacagtgca accattgctc cataaatatg tgtaaggtgc
     1441 attgcctccg cttgtttgtg cagatattat gcctgttgag tcgccaaaac aatatacagg
     1501 ttgcgccaat gcattaactt gcaagggcgc agcacatact gaaccacata agcccattaa
     1561 ttgtgaatac tgattgcaaa gccaatcgaa attttgttgt gcatcagaa
//
LOCUS       DAOMAA010000549         1604 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_86743_length_1604_cov_2.767592, whole genome shotgun sequence.
ACCESSION   DAOMAA010000549 DAOMAA010000000
VERSION     DAOMAA010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86743_length_1604_cov_2.767592"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="PLO59_07545"
     CDS             complement(<1..485)
                     /locus_tag="PLO59_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010601986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQV00997.1"
                     /translation="MSTIKCLIIGSGPAGYTAAIYAARADLKPTMIAGLQPGGQLTIT
                     TEVENYPGYPDGTQGPQMMEDFRKQAMRFGTDIKWSYISAVDFSSRPFKAQVDDKDWI
                     TAESVIIATGASAKWLGLPSEQRLNGYGVSACAVCDGFFFKGQDVAIVGAGDTAAEEA
                     T"
     gene            complement(607..1128)
                     /locus_tag="PLO59_07550"
     CDS             complement(607..1128)
                     /locus_tag="PLO59_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013634688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HQV00998.1"
                     /translation="MAHYSAALMATQLLNETQIENRIRRIAFQIYEDNFEADEIILAG
                     IVKGGYVLAAEIEKRLKEICEIKITLIEINLDKNSQAQKPIQVALPNDYLQNKTVVIV
                     DDVLNSGKTLTYAMLPFLNADVKKIRTVLLVDRDHKRYPVHADFVGFELSTTLKEHVT
                     VNLTPGNKRIMLS"
     gene            1242..>1604
                     /locus_tag="PLO59_07555"
     CDS             1242..>1604
                     /locus_tag="PLO59_07555"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HQV00999.1"
                     /translation="MKVTTTYNQILKIALPITLGGVAQTILNLTDTAFLARVGEVELG
                     AAAIGGVFYFVLVMLGYALGTGAQIIISRRAGEGNTGAIGIVFDHTLILLAGFGVLLF
                     AFMLCSPVIINSIISDDAI"
BASE COUNT          460 a          309 c          328 g          507 t
ORIGIN      
        1 taggtggctt cttcggctgc ggtatcgcct gcgcctacaa tggcaacatc ctgtccttta
       61 aaaaagaaac catcacaaac ggcacatgcc gaaacgccat agccatttaa gcgctgctcg
      121 ctaggcaagc ctaaccattt tgccgaagca ccggtggcaa taattacact ttcggccgtt
      181 atccaatctt tatcatctac ttgtgcttta aacgggcggc tgctaaaatc aactgcactt
      241 atataactcc atttaatatc ggtaccaaag cgcatggctt gttttctaaa atcttccatc
      301 atttgcgggc cttgtgtacc atcggggtaa ccgggatagt tttcaacttc ggtagtaatg
      361 gtaagttggc caccgggttg taagccggca atcatggtag gttttaaatc ggcacgggcg
      421 gcatagatgg ctgctgtgta accggcaggc cctgaaccta ttatcaggca ttttattgtt
      481 gacatcagat taaatttttt aaaactattg attatttaaa gcggggcaaa agtaaatttt
      541 aatgtattgt ttgatgacgg atgtaagtcg aaaaatcatc agcttgaaat acttttttaa
      601 caaagtttag ctaagcataa tgcgcttgtt gccgggagtt aaattaaccg ttacatgttc
      661 ttttaaagtt gtcgaaagct cgaagcccac aaaatcagca tgaacgggat agcgcttatg
      721 gtcgcgatcg accaacaaaa cagtccgtat tttttttaca tcggcattaa gaaatggcaa
      781 catggcgtag gttagggttt tgcccgagtt taaaacatca tcaactataa caacagtttt
      841 gttttgcaaa taatcatttg gcaacgccac ctgaattggt ttttgagcct gactgttttt
      901 atccaaatta atttcgataa gcgtaatttt tatttcacaa atctctttta atcttttttc
      961 gatttcggct gcaagcacat aaccaccttt tacaatgccg gctaaaataa tttcatcagc
     1021 ttcaaagtta tcttcataaa tctgaaaagc gatacgcctg atgcggtttt caatttgcgt
     1081 ttcgtttaag agctgtgttg ccataagtgc tgccgaataa tgagccaaat attttatacg
     1141 taagtacaat gcaacaaata aagtaaatga cttaggtaat tgctaataaa tataagttcc
     1201 ttagcggcct ttttgcaaag tttacctata accgaacaaa attgaaagta acaaccacat
     1261 acaatcaaat attaaaaatt gcgctgccca taacattggg tggtgttgcg caaaccatat
     1321 taaatttaac cgataccgct tttttagcgc gtgttggcga agttgaatta ggtgctgctg
     1381 caattggtgg tgtgttttat tttgttttgg ttatgttggg atatgcttta ggcaccggtg
     1441 cgcaaataat aatttcgcga agggcaggcg aaggcaatac aggcgccata ggcattgtgt
     1501 ttgatcatac cttaatttta ctggcaggct ttggcgtttt gttgtttgca tttatgctgt
     1561 gttcgcctgt aataataaat agtataatca gcgatgatgc cata
//
LOCUS       DAOMAA010000550         1604 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_86748_length_1604_cov_2.632666, whole genome shotgun sequence.
ACCESSION   DAOMAA010000550 DAOMAA010000000
VERSION     DAOMAA010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86748_length_1604_cov_2.632666"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1604
                     /gene="sprA"
                     /locus_tag="PLO59_07560"
     CDS             <1..>1604
                     /gene="sprA"
                     /locus_tag="PLO59_07560"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HQV01000.1"
                     /translation="TLKFPFTDRYSDPISSQYHNSPLFLSDPSNIKTQVTYDPNSNQY
                     NINEMIGDEFYRNPSYLTYEEYLRKSNNESTKKYWKQLAEGGNSISGKRGLNPRLYVG
                     SEVFERIFGSNTIDIRPQGSAELTFGANIATNRNPAIPTRQQRVATFDFKQKIQLNVV
                     GNIGDKMKLSTNYNTEASFDFENTMKLEYTGHDDDIIKKIEAGNVTLPLTGTLITGSQ
                     SLFGFKTQLQFGKLVFTGLFSQQKGKSQVINVPPGGGQKTDFEVTADNYEANRHFFLG
                     QYFRNRYDEALKNAPQITSPVIITKCEVWVVKPGLTDTRSLIGFADLGEDVPLSQSPI
                     VQSPNPIIPGALPNNFANNLYANMSDTTQVYYNLRNPNYANATAVFNDLTNQGFVAPR
                     DYERIENAVKLQPNEFTLNPTLGYISLKSQLNPNQVLAVAYEYSHNGQIYRVGDLTIA
                     GINPPNRLFVKILKSTNVNQLYPVWDLMMKNIYSLGAYGLTQKDFFLNVLYYNDSTGA
                     DINFLGAGATEPDVNGIPLIRVLNLD"
BASE COUNT          514 a          363 c          309 g          418 t
ORIGIN      
        1 tactttaaaa tttcctttta ccgacaggta ctccgacccc atatcgtcac aataccacaa
       61 cagcccgtta tttttaagcg acccatccaa tataaaaact caggtaacgt acgaccccaa
      121 cagcaatcag tacaatatca acgaaatgat tggtgatgag ttttaccgta accccagtta
      181 tttaacgtat gaagagtatt tacggaaaag caataacgaa agcactaaaa aatactggaa
      241 acaattggcc gaaggcggca acagcataag cggcaaacgc ggccttaacc ccagactata
      301 tgtaggcagc gaagtttttg aacgcatttt tggtagcaat accattgata tacgcccgca
      361 aggttcggcc gagctaactt ttggggctaa cattgccacc aaccgcaatc cggccatacc
      421 tacacgccag caacgtgtgg caacattcga ctttaaacaa aagatacaat tgaatgtggt
      481 gggtaatatt ggtgataaaa tgaaactgag caccaactac aataccgaag ccagtttcga
      541 tttcgaaaac accatgaagc tggaatatac aggccatgat gatgatatta taaaaaagat
      601 agaagccggt aacgttacct tgcccttaac cggtacttta attacaggaa gccaaagttt
      661 atttggtttt aaaacacaat tgcaatttgg caagctggtt tttacaggtt tattctcaca
      721 acaaaaaggc aagtcgcagg ttataaacgt accacccggt ggcggacaaa aaaccgactt
      781 tgaagttaca gccgataatt atgaagccaa ccgccatttc tttttaggcc aatatttcag
      841 aaaccggtat gatgaagcat taaaaaatgc gccacaaatt acttcacccg taattattac
      901 caaatgcgaa gtttgggtgg taaagccggg cttaaccgat acgcgcagct taattggttt
      961 tgccgatttg ggcgaagacg tacccttatc gcaatcgccc attgtacaaa gccccaatcc
     1021 gataataccg ggtgctttgc ctaacaactt tgccaacaac ttgtacgcca atatgagcga
     1081 tacaacgcaa gtttattaca acctgcgcaa tcccaattat gccaatgcta cggctgtgtt
     1141 taacgattta accaaccagg ggtttgtggc accacgcgat tacgagcgta ttgaaaatgc
     1201 cgttaagttg caacccaatg agtttacgct taatccaaca ttgggttata tttctttaaa
     1261 gtcgcagtta aatcccaatc aggttttagc agtagcatac gagtacagcc acaatgggca
     1321 aatttaccgt gtgggcgatt taaccatagc aggtattaat ccacccaacc gcttgtttgt
     1381 aaaaatcctg aaaagcacca acgtaaatca gctttacccg gtttgggatt tgatgatgaa
     1441 aaacatctat tcattaggcg cttacggctt aactcaaaaa gattttttct taaacgtatt
     1501 gtactataac gactcaaccg gtgccgacat taatttttta ggtgctggtg ccaccgaacc
     1561 cgatgttaat ggcatccctt taattcgtgt acttaactta gacc
//
LOCUS       DAOMAA010000551         1604 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_86772_length_1604_cov_2.226598, whole genome shotgun sequence.
ACCESSION   DAOMAA010000551 DAOMAA010000000
VERSION     DAOMAA010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86772_length_1604_cov_2.226598"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /locus_tag="PLO59_07565"
     CDS             <1..981
                     /locus_tag="PLO59_07565"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HQV01001.1"
                     /translation="ASVLLEKDYKTKYDGYDPNSPESHIIFNLYQNVYMEKSLTFASK
                     VQANFEEYANRYNRGVKQAGFLVLYKTAMPSVLIETGFLTNANDEKYLLSDKGQEQTA
                     TAIYNAFKEYKVDMEEGDAQPGSTQTKSSEATNTTVIEANQNEVKATTSNKNTDTVKV
                     GASDTNAKPKNSAIEPVANTKPATNSNSKTTDTGIIASAKKADSLAHAKTHKQPVVKT
                     QEKSEARTDIKAPPKSPEGIDEALFYTIQIGATPILTEDEKLKYANSGARIITSEDGK
                     MLRITMGHYKTLHQAQQQQKILKTKGYNDAFITPYHQGKRITLKQAADIK"
     gene            1069..>1604
                     /locus_tag="PLO59_07570"
     CDS             1069..>1604
                     /locus_tag="PLO59_07570"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HQV01002.1"
                     /translation="MISKYSKEIKVGLIVTCAIAAGIWGINYLKGQDLFSRYKNFYVV
                     YENIDGLAQSNMVRINGYKVGSVYDVSFLPDNSGRILIELEVAASALINKDAQALITN
                     ADILGTKEIRLVLCKSTEQANDGDTLYGKVATGMMAQIEPVKDKVEMLVGSLDSLIRN
                     LNSVISPGNKVHIESSIIS"
BASE COUNT          622 a          279 c          303 g          400 t
ORIGIN      
        1 gcctcggttt tgcttgaaaa ggattataaa accaaatacg atggatatga cccaaactcg
       61 cccgaaagtc atataatatt taacttgtat caaaatgttt acatggaaaa aagcttaacc
      121 tttgccagta aagtacaagc caatttcgaa gaatatgcca accggtataa caggggtgta
      181 aaacaagccg gctttttagt tttatacaaa accgctatgc ccagtgtact tatcgaaacc
      241 ggttttttaa ccaatgccaa tgatgaaaag tatttgctaa gcgataaagg acaagaacaa
      301 acggctacag ctatttacaa tgcctttaaa gagtataaag ttgatatgga agaaggcgat
      361 gcacaacccg gtagtacaca aacaaaatca agtgaagcaa ctaatacaac cgtaattgag
      421 gctaatcaaa acgaagtaaa agcaaccact tcaaataaaa ataccgatac agttaaagtt
      481 ggcgcatcag atacaaatgc caaaccaaag aacagtgcta ttgaaccagt agcaaataca
      541 aaacctgcta ctaattccaa ttcaaaaaca accgacaccg gcattatagc atcagctaaa
      601 aaagccgata gcctggccca tgcaaaaaca cataaacagc ctgtggttaa aacgcaagaa
      661 aaatcagaag cccgaaccga tattaaagcg ccacccaaat cacccgaagg tattgatgaa
      721 gctttatttt ataccattca aattggggca acaccaatat taactgaaga cgaaaaatta
      781 aaatatgcca atagtggcgc gcggataatt acatcagagg atggaaaaat gctgcggata
      841 accatgggcc attataaaac attacatcaa gcacagcagc aacaaaaaat tttaaaaaca
      901 aaaggttaca acgatgcctt tattacaccc taccatcaag gcaaacgaat tacattaaag
      961 caagcagccg atattaaata aagtgctagt tgcagttgga taactgaacc gaaagtaaat
     1021 ttgaagggtt attaatattg atagatttga aaaattaatt aaacatacgt gataagtaaa
     1081 tactcaaaag aaattaaagt aggcttaata gtaacatgtg ccattgcagc aggcatttgg
     1141 ggcataaatt atttaaaggg acaggatctg ttttcgcgat acaaaaactt ttatgttgtt
     1201 tatgaaaaca ttgatggatt ggcgcagtcg aatatggtac gcattaatgg atacaaagta
     1261 ggctcagtat atgatgtatc gtttttgccc gataactcag gccgtatatt aattgaatta
     1321 gaagtagctg ccagtgcatt aattaataaa gacgctcagg cattaattac caatgctgat
     1381 atattaggta ctaaagaaat cagattggtt ttgtgtaaaa gtacggagca ggcaaatgat
     1441 ggcgatactt tatacggaaa agtagcaacc ggtatgatgg cacaaattga acctgtaaaa
     1501 gataaagtgg aaatgctggt tggatcgctg gactcattaa ttcgaaattt aaatagtgtt
     1561 ataagtccgg gtaataaagt tcatattgaa agcagcatta tttc
//
LOCUS       DAOMAA010000552         1599 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_87243_length_1599_cov_2.722150, whole genome shotgun sequence.
ACCESSION   DAOMAA010000552 DAOMAA010000000
VERSION     DAOMAA010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87243_length_1599_cov_2.722150"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="PLO59_07575"
     CDS             <1..478
                     /locus_tag="PLO59_07575"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV01003.1"
                     /translation="TVYLDILQGQKLSASQKNIIHKMAFQCPYTYGNSVFKARAINAI
                     YNRKYNYNDAQLCKGKYAVDENNISNLTNDDLYVGLYPNPNSGSFHLKYDLSKLAEAQ
                     IQFYDLMGKVLRTEKINITTNTQNFSLSNLANGVYYYAIYSNDQMVYRSKFTIINK"
     gene            547..1104
                     /locus_tag="PLO59_07580"
     CDS             547..1104
                     /locus_tag="PLO59_07580"
                     /inference="COORDINATES: protein motif:HMM:NF025167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HQV01004.1"
                     /translation="MFRIFFTEGFYHIVSWEAIDHILYLLVLVVSFDISKWRSILLLV
                     TTFTVAHTLALFVSVAGLFYLHSNLVEVLIAITIAATCIENLISKKILWVSVLTALCF
                     GFIHGLGFSATLTTMFNHIDINYFETLLPFNLGVECGQIVVLIPILSVLFYLSKYTGM
                     STKQKSKLISWCVLIVSCVFIMQRV"
     gene            1164..>1599
                     /locus_tag="PLO59_07585"
     CDS             1164..>1599
                     /locus_tag="PLO59_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010177513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HQV01005.1"
                     /translation="MKIICVGRNYAEHVKELGNQQNSEPVVFLKPDTAIPQKDMPFFY
                     PGFSNDVHHEIEVVIKINRVGKNIAPKFANTYYDEVTVGIDFTARDLQAELKSKGLPW
                     EISKGFDGSAIVGSFVSKSQLPALNNLEFRLLKNSMLVQHGHT"
BASE COUNT          527 a          219 c          289 g          564 t
ORIGIN      
        1 tactgtatat ctggacattt tacaggggca aaaattatca gccagccaaa aaaacatcat
       61 ccataaaatg gcatttcaat gtccttatac ttatggtaac agtgtgttta aagcgcgtgc
      121 aataaatgcc atttataata gaaaatataa ttataacgat gcgcaacttt gtaagggtaa
      181 atatgcagtt gacgaaaaca acataagtaa tttaacaaac gatgatttat atgtagggct
      241 gtatccgaat ccaaatagcg gttcctttca tttaaaatat gatttatcaa aattagctga
      301 agcccaaatc caattttacg atcttatggg taaagttttg cgtacagaaa agattaatat
      361 aactactaat acacaaaatt tcagtttgag taatttagca aatggagtat attattatgc
      421 tatatatagt aacgatcaaa tggtatatcg aagcaaattt acaatcatca ataaataagt
      481 tattactttg ttcggttaaa aagcctgcat atttgcaggc tttttttatg tattaaaaac
      541 agtattatgt ttcgtatatt ttttactgaa gggttttatc acatagtttc atgggaagca
      601 attgatcata ttttatacct gttggttctt gtggtttctt ttgatataag taaatggcgt
      661 agtattttat tattagttac aacatttaca gtagcccata ccttggcgtt atttgtatcg
      721 gtagctggtt tgttttattt gcatagtaat ttagttgaag tgttaattgc aattacaata
      781 gctgctactt gtatagaaaa cctaataagt aaaaagatac tttgggtaag tgtgctaaca
      841 gctttgtgtt ttggctttat acatggcttg ggttttagtg caacacttac aaccatgttt
      901 aatcatattg atattaacta tttcgaaact ttattgcctt ttaacttagg tgttgagtgc
      961 ggtcagatag tggtacttat accaatactt tcagtgctgt tttatttaag taaatatacg
     1021 ggcatgtcaa caaagcaaaa aagtaagttg atttcgtggt gtgttttaat tgtatcatgt
     1081 gtgtttataa tgcaaagggt ttaaatttaa acaacaactc aattgcgctt aattttacag
     1141 atgttattta taagtttaat caaatgaaaa ttatttgtgt tggccgcaac tatgctgagc
     1201 atgtaaaaga gttaggcaat cagcaaaatt cagaaccggt tgttttttta aaacctgata
     1261 cagctattcc tcaaaaggat atgccatttt tttatccggg ttttagtaat gatgttcatc
     1321 atgaaattga agtggtgatt aaaattaacc gtgttggaaa aaatatagca ccaaaatttg
     1381 caaacactta ttatgatgaa gtaactgttg gcattgactt taccgcacgc gacttgcaag
     1441 ctgagttaaa gtcgaaaggg ctcccatggg aaatatcaaa aggttttgat ggttcggcta
     1501 ttgttggaag ctttgtttca aaatcgcaat tgcctgcatt aaacaatttg gaatttaggt
     1561 tacttaaaaa tagtatgcta gtgcagcatg ggcacacat
//
LOCUS       DAOMAA010000553         1599 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_87246_length_1599_cov_2.585492, whole genome shotgun sequence.
ACCESSION   DAOMAA010000553 DAOMAA010000000
VERSION     DAOMAA010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87246_length_1599_cov_2.585492"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..142
                     /locus_tag="PLO59_07590"
     CDS             <1..142
                     /locus_tag="PLO59_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015807449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HQV01006.1"
                     /translation="IITMVLDEVEYLDIAQVFGISEGNLRVKIHRIKQKLTEIYNYHA
                     RL"
     gene            129..725
                     /locus_tag="PLO59_07595"
     CDS             129..725
                     /locus_tag="PLO59_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01007.1"
                     /translation="MQDFEALKKMWQLNQEQQAMPEANDILLQVSNTKNVLAKNLLKA
                     VWQLIPAMVIVILIAVFIKFVNIITYVGIILVLVSIVVYGAALIWHYFNLSKDYSTLK
                     PADYLKVIQHQYEIRKKFNQIGGYLYSLILYIGSMLYLVEVTAHVTPLWQWFGFIITT
                     VWFLYVVFVLSKKVIAYENQKFEDIITQLKKLNQQLET"
     gene            760..1530
                     /locus_tag="PLO59_07600"
     CDS             760..1530
                     /locus_tag="PLO59_07600"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HQV01008.1"
                     /translation="MQIEWLWILLCLTALLAGFTDAVVGGGGLIQLPVLFLVFPAWLP
                     VNIIATGRLAGACGTFVAAFQYVKKVQVKWPWLLATMVAAMLMALVGAFAMKQTPPQT
                     FKPLVFIVLVVMALYTFAKKQWGFAHHPSTVSKPVLWAGLIGMIIGFYNGYMGPGTGS
                     LLVFAYISILKHDFLHASASAKVINFFTDLSTIIYFIFIDAIAYKIAIPMAICNVAGG
                     YLGSHLAIAKGSAFVRVFFMILMIVLILRFGYEIFIVR"
BASE COUNT          470 a          242 c          323 g          564 t
ORIGIN      
        1 gataattacc atggttttag atgaagtaga atacctggat atagcgcagg tttttggtat
       61 atcagaaggt aatttgcgtg taaaaattca tcgcatcaaa caaaagttaa ccgaaatata
      121 taactaccat gcaagacttt gaagcgttaa aaaagatgtg gcagttaaat caggagcaac
      181 aggctatgcc cgaagcaaat gatattttat tgcaggtatc caacacaaaa aatgtgcttg
      241 ccaaaaattt gcttaaagcg gtttggcaac tcataccggc tatggttatc gtaatattaa
      301 ttgccgtttt tataaaattt gtaaacatta ttacttatgt aggtatcata ctggtgttgg
      361 tttcaattgt tgtatatggt gcagcattaa tatggcatta ctttaatctg tcaaaagatt
      421 acagtacttt aaaacctgcc gattatttaa aagtgataca acatcaatat gaaatccgta
      481 aaaagtttaa tcagataggt gggtatttat attcgctgat attatacata ggcagcatgc
      541 tgtatttagt agaagttact gcacatgtaa ccccattgtg gcaatggttt ggatttataa
      601 ttactactgt ttggtttcta tatgttgttt ttgttttaag taaaaaggtt atcgcatacg
      661 aaaaccaaaa gtttgaagat attattaccc aattaaaaaa acttaaccaa caattagaaa
      721 cgtaacagca ttaaacagca tcttttttaa gtttgcctga tgcaaattga gtggctgtgg
      781 atactacttt gtttaaccgc tttattagca ggttttactg atgccgttgt tggtggtggt
      841 ggtttaattc aacttccggt tttgttctta gtttttccgg catggctacc ggtaaatatt
      901 attgcaacgg gtcgtttggc gggtgcatgc ggcacatttg tagcagcttt tcaatatgta
      961 aaaaaagtac aagtaaaatg gccttggcta ctggcaacta tggtagctgc catgttaatg
     1021 gctttagttg gcgcttttgc tatgaaacaa acaccaccgc aaacatttaa gccgctggtt
     1081 tttattgtgc tggttgtaat ggctttgtac acatttgcta aaaagcaatg gggctttgcc
     1141 caccatcctt caacagttag caaacctgtt ttgtgggccg gtttaatagg aatgattata
     1201 ggcttttata acggatatat ggggccgggt acaggcagtt tactggtatt tgcatacatc
     1261 agtattttaa aacatgattt tctgcatgct tcggcatcgg ctaaagtcat taattttttt
     1321 acggatttaa gcaccatcat ctattttata ttcatagatg ccattgcata taaaattgca
     1381 attccgatgg ctatttgtaa tgtggcaggt ggctatcttg ggtcgcattt ggcaattgca
     1441 aaagggagtg cctttgtaag agtttttttt atgattttaa tgattgtact tattcttagg
     1501 tttgggtatg aaatttttat tgttagataa tgaatagttt ttttcgcacc atatttttta
     1561 tctggttttt gtttagcaca aacgtaggtg ttgcacaat
//
LOCUS       DAOMAA010000554         1597 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_87489_length_1597_cov_2.398833, whole genome shotgun sequence.
ACCESSION   DAOMAA010000554 DAOMAA010000000
VERSION     DAOMAA010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87489_length_1597_cov_2.398833"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..>1597
                     /locus_tag="PLO59_07605"
     CDS             16..>1597
                     /locus_tag="PLO59_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01009.1"
                     /translation="MNLFYYYDGTAWRPFMIGNLSWLLSGNAGTNPTTQFVGTTDAVG
                     LAFRTGNVEAMRISQTGNIGVGTTTPVASALLEMTATNRGILIPRLTTAQRTAIVTPA
                     TGLLVYDSSLNLFYYFNGVIWVPLQSNLGWLLTGNAGTNPTVNYLGTTDAQSLVIRAN
                     AIEAIRVLATNRNVGIGQVAPVSKLDVNGNITIGAGYSGLIAPTNGAIIQGHTAVGTF
                     TPLANDVFSSYAAGSLIAVAGYATGSGNAAYFQNNSTGTVGRFLKTTGAAGSALQVHT
                     NSAANSATVLEVAGNGTGNTVVIADTNTTGRSLLINKTNTTTTATTIDINNAGRGYSI
                     HAKNANTANTNAGVRIEQMRGDGTYTLMGAGNANIGNSIYHNGTGIGQKIEMAPGNTG
                     IGLQIIHAGNEKGIDVSLSNVANSNDAVKINHAGTGKGIIVTVASPLSYGIWASVSNT
                     VPAINPGFSLAGPPPVTTAFANGVGIITAAPGHGIIANSVLDASNITDHDGGIFIVRQ
                     DNISSTNTAASSVGARIGN"
BASE COUNT          478 a          333 c          358 g          428 t
ORIGIN      
        1 gtatatgata ctactttgaa tttattttat tactacgatg gtacagcctg gcgtccattt
       61 atgattggta acctaagttg gcttttaagt ggtaatgctg gcaccaatcc tactacacag
      121 tttgttggca ctaccgatgc cgtgggtctg gcttttagaa ccggtaatgt tgaggcaatg
      181 cgcataagtc aaaccggcaa cattggtgtt ggaactacaa caccggttgc ttcggcttta
      241 cttgaaatga cagctaccaa ccgtggtatt ttaattccac gtttaacaac agcacaacgc
      301 acggccattg ttacaccagc tacgggtttg ttagtttatg atagcagctt aaatttattt
      361 tattacttca atggcgtgat ttgggtgcca ctacaaagta atttgggatg gctattaacc
      421 ggaaatgcag gcaccaatcc tacggtaaat tatttgggaa ccaccgatgc acaatcattg
      481 gtaataagag ctaatgctat tgaggctatt cgcgttttgg caaccaacag aaatgttgga
      541 ataggacagg tagcccccgt ttcgaaatta gacgtgaacg gcaatataac cataggcgca
      601 ggctatagtg gactgattgc accaacaaac ggggcaataa ttcaaggaca tacagccgtt
      661 ggcactttta caccattggc caacgatgtt ttttcatcct atgcagccgg atcattaatt
      721 gctgtggccg gttatgctac aggttccggc aatgcagcct attttcaaaa caatagtaca
      781 ggcacagtag gtcggttttt aaaaacaacc ggtgctgccg gtagcgcatt gcaggtgcat
      841 accaatagtg ctgccaatag tgcaacagtt ttagaagtag cgggcaatgg taccggtaac
      901 accgtagtta ttgccgatac caatactacc ggacgcagtt tattgattaa taaaaccaat
      961 actacaacaa cagctacaac cattgatatc aataatgcgg gcagaggtta ttccatacat
     1021 gcaaaaaatg ccaatacggc taacacaaat gcaggtgtgc gcatcgaaca aatgcgtggc
     1081 gatggaactt atacattaat gggtgcaggc aatgcaaaca ttggcaattc catttatcat
     1141 aatggcacag gcataggaca aaaaattgaa atggcacccg gtaataccgg cattggttta
     1201 caaataatac atgcaggtaa tgaaaaaggt attgacgtat cgttatcgaa tgtagccaac
     1261 tctaatgatg ccgtaaaaat taaccatgca ggtacaggca agggtattat tgtaacagtt
     1321 gccagcccat tatcctatgg tatttgggcc agcgtaagca atacagtacc tgctataaat
     1381 ccggggttct cattggcagg tccaccaccg gtaacaacgg cttttgcaaa tggcgttggt
     1441 ataattaccg ctgcacccgg ccatggtatt attgcaaaca gcgtattgga tgcatcgaac
     1501 ataaccgacc acgatggcgg aatttttatt gtacgtcaag ataatatttc ttcaaccaat
     1561 acagcagcct caagtgtggg tgcgcgtatt ggtaatg
//
LOCUS       DAOMAA010000555         1593 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_87864_length_1593_cov_2.638492, whole genome shotgun sequence.
ACCESSION   DAOMAA010000555 DAOMAA010000000
VERSION     DAOMAA010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87864_length_1593_cov_2.638492"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1131)
                     /locus_tag="PLO59_07610"
     CDS             complement(<1..1131)
                     /locus_tag="PLO59_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01010.1"
                     /translation="MKRIKEPVNKSNMLKWLCLVLLIALSINNLKGQTGFHVLHIDSR
                     KGLPQNHIHYITFDKNGFMWGLSEMGIFRYDGIRFTEFKANKNSQLLDNNYSGFIQMF
                     NGDLAIADIHGGVIEIDSANITKVSGSNINKHEVVKINGLFPDLNTYSRIFNADKTNI
                     NKDGQIELPLVYTFNKRQHLTANNHKVFFYLDNKLSDSITLPDSNCNWIFLSNRLFYF
                     NNSNDLFELKFDAPNFVATQPVFSEALKRKKNDKLKIFTNDAKGSCLQINNSVYYVTI
                     VNNLIVSKLIIDSLPQRVDITNIIYCDKNKAAVVSTTSTGIFIYKAQPFKSIIYNGAD
                     INATNSFYSQVYLDSTTILTDWNLEVNRNGAQKSKRNIHRNYE"
     gene            complement(1103..>1593)
                     /locus_tag="PLO59_07615"
     CDS             complement(1103..>1593)
                     /locus_tag="PLO59_07615"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQV01011.1"
                     /translation="THLILDMQMQDGNLLDIISNITGNYPQIQIMIYTMNSEDLYGLR
                     MLKLGAHIFLNKQTHETDLKQALQAFLNNQPYVSSHLSDLINDNKKRKNKDAFASLSD
                     RELTVLNNILAGKSLKEIAINLDLKANTVATFKARIFDKLGVSNLIDLKAVVETYQRT
                     GK"
BASE COUNT          492 a          255 c          258 g          588 t
ORIGIN      
        1 ttcataattg cgatgaatgt tgcgtttcga tttttgtgct ccatttctgt ttacttccag
       61 attccagtcg gttaatatgg ttgtactatc taaatacacc tgactgtaaa aggaattggt
      121 tgcattaatg tcagcaccgt tatatattat acttttaaaa ggttgtgctt tataaataaa
      181 aattccggtt gatgttgtgc taacaactgc ggccttattt ttatcgcaat aaattatatt
      241 ggttatgtca actctttgcg gtaacgaatc tattatcagt ttacttacta ttaaattgtt
      301 tacaatagta acatagtaaa cactgttgtt tatttgcagg caactgcctt tagcatcgtt
      361 agtaaatatt tttaatttat cgtttttttt tctttttaaa gcttcactaa aaacgggttg
      421 tgttgcaaca aaatttggag cgtcaaattt taattcaaaa aggtcatttg agttattgaa
      481 atagaataaa cgattgctta aaaagatcca attgcaattt gaatcaggca atgtaatcga
      541 gtctgatagt ttgttgtcca aataaaaaaa cactttatgg ttgtttgcag tcagatgctg
      601 ccttttgtta aaagtataga ctaagggcag ttctatctga ccatccttat ttatattggt
      661 tttatctgca ttgaatattc ttgaataagt attcaggtcg ggaaacaggc catttatttt
      721 tacaacttca tgtttgttaa tgtttgagcc ggatactttt gtaatgtttg ccgaatcaat
      781 ctcaataaca ccaccatgta tatcggcaat agctaaatcg ccattaaaca tttgtataaa
      841 accactatag ttattatcta atagctgcga atttttattg gctttaaatt cggtaaaccg
      901 aatgccatca tatctaaaaa tacccatctc acttaaaccc cacataaaac catttttatc
      961 aaaagttata tagtggatgt ggttttgtgg taaacccttg cgcgaatcaa tatgcaaaac
     1021 atgaaagcct gtttgcccct tcaaattatt tattgagagt gctattaaaa gaaccagaca
     1081 caaccattta agcatgttac ttttatttac cggttctttg atacgtttca actactgctt
     1141 ttaagtcaat cagattagaa acgcctaatt tgtcaaatat ccgggcttta aaagtggcaa
     1201 cggtgtttgc ttttaagtcg agattgatag caatttcttt taaactctta cctgcaagaa
     1261 tattatttag cacggtaagt tctctatcgg aaaggctggc aaaagcatct ttgttttttc
     1321 ttttcttatt gtcatttatt aagtcggata aatggctgct tacataaggt tgattattta
     1381 aaaatgcttg caaagcctgt tttaaatcgg tttcatgcgt ttgtttatta agaaaaatat
     1441 gagcacctaa tttaagcatc cgtaagccat acaaatcttc tgaattcatg gtataaatca
     1501 ttatctgaat ttggggataa ttaccggtaa tgtttgaaat aatatctaac agattgccat
     1561 cctgcatctg catatctaaa attaaatgtg tgt
//
LOCUS       DAOMAA010000556         1592 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_87944_length_1592_cov_3.467144, whole genome shotgun sequence.
ACCESSION   DAOMAA010000556 DAOMAA010000000
VERSION     DAOMAA010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87944_length_1592_cov_3.467144"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1592
                     /locus_tag="PLO59_07620"
     CDS             <1..>1592
                     /locus_tag="PLO59_07620"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HQV01012.1"
                     /translation="GKRKKTTEFYKALENFDTYPYRHQLLALYPPELLAACQALQNRL
                     FTAIYLPDYAGKNVVTLHELSQDKVVGLQHKMNECLQTLFNHANTTPFYIDNHTENNT
                     LPDVDFKTTYTLSVTPVHASLSVRFDNQYMVLKPQLKMGADFSEPTDFEIITPWLLLR
                     QGTIYKLNSYADLSLLQKFEKYESLKFKIIDKQTLFNQVLQPLSKNNVLQFENTLVET
                     ISAKPTQIHLYLKEFEKVLLFVPKFIYTVADQTVETDLHTDNDALVYNHDKVYLIKRD
                     LESETEATRFFKTLHPDFEQNHTEPYFHLTIEQVMHQGWFFKLFEQLKQNNIAVFGQQ
                     ELKKIKYNTHKPIITFGNSSGTDWLDLQLKITFGQTQVSLNQVRKAILNNQKFVPLDD
                     GTVGLLPDEWVTRYAPLFKYGKLDKTKLSISKLHFTIIDTYLNEINNDALAVELQVKK
                     NKLLQFESMQHQQVPAQIQAQLRPYQVTGFEWLHFLNDFNWGGCLADDMGLGKTLQVL
                     TLLQHLKNNQKLKTSLVVVPTT"
BASE COUNT          558 a          309 c          281 g          444 t
ORIGIN      
        1 ttggtaaacg aaaaaaaaca actgaatttt ataaagcact cgaaaatttt gacacttatc
       61 cttacaggca tcaattgctg gcattatacc caccggagct tttagcagcc tgccaggctt
      121 tgcaaaacag gctgtttact gcaatttacc tgcccgatta tgccggaaaa aatgtagtga
      181 cgctgcacga attgtcgcaa gataaagtag tgggcttgca acataaaatg aatgaatgct
      241 tacaaacact ttttaaccat gcaaacacta caccttttta tattgacaat catactgaaa
      301 acaacacctt acctgatgtt gattttaaaa caacctatac cctaagtgta acaccggtgc
      361 acgccagctt aagtgtgcga tttgacaacc agtacatggt tttaaaacca caattaaaaa
      421 tgggtgctga tttttcagag ccaaccgact tcgaaataat tactccctgg cttttattac
      481 gccaagggac tatttataaa cttaatagtt atgccgacct gagtttgctg caaaagtttg
      541 aaaaatatga atcgctaaaa tttaaaatta ttgacaagca aacattgttt aatcaagtat
      601 tacaaccact gtcaaaaaac aatgttttac agttcgaaaa tactttagta gaaaccatat
      661 cagcaaagcc aacacaaata catttatatt taaaggagtt tgaaaaagtt ctgctgtttg
      721 ttcccaaatt tatttacacc gttgccgacc aaactgtaga aaccgacttg cataccgata
      781 atgatgcttt ggtatataac catgacaaag tgtatttgat aaaaagagat ttggagtccg
      841 aaacggaagc aacccgtttt ttcaaaacat tgcatcccga tttcgaacaa aatcataccg
      901 aaccatattt ccatttaacc attgaacagg taatgcacca ggggtggttt ttcaaattat
      961 tcgaacagct taaacaaaac aacattgctg tatttggcca gcaagagctt aaaaaaataa
     1021 agtataatac acataaaccc ataatcacat ttggtaatag cagcggaacc gattggcttg
     1081 atttacaact aaaaataacc tttgggcaaa cccaggtttc attaaaccag gtgcgtaaag
     1141 ccattttaaa taatcaaaaa tttgtgcctt tagacgatgg taccgttgga ctattgcctg
     1201 atgaatgggt aacgagatat gccccattat ttaaatatgg caagctcgat aaaacgaagt
     1261 taagcatcag caaattacat tttacaataa ttgatacgta cttaaacgaa attaataatg
     1321 atgcattggc tgttgagttg caggttaaaa aaaacaaact gttgcagttc gaaagcatgc
     1381 aacaccagca agtgccggcc caaatacagg cgcagttgcg gccttatcag gtaaccggtt
     1441 ttgaatggct gcatttttta aacgatttta actggggtgg atgcctggcc gatgatatgg
     1501 gcttaggtaa aacattgcag gtgcttacct tattacagca cctgaaaaac aaccaaaagc
     1561 ttaaaaccag tttggtggtt gtgcctacaa ca
//
LOCUS       DAOMAA010000557         1592 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_87977_length_1592_cov_2.699414, whole genome shotgun sequence.
ACCESSION   DAOMAA010000557 DAOMAA010000000
VERSION     DAOMAA010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87977_length_1592_cov_2.699414"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(205..624)
                     /gene="ssb"
                     /locus_tag="PLO59_07625"
     CDS             complement(205..624)
                     /gene="ssb"
                     /locus_tag="PLO59_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014796207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HQV01013.1"
                     /translation="MSGLNKVLLIGNLGKEPEIKHLEGGYAKATFPLATTENYKNKDG
                     IKQEQTEWHTIVAWRALAESIEKLQLKKGTMLYVEGKIRNRTYDDKEGHKKSYTEINA
                     DSITVIKRPAIVENGSATEVKSAPVPDGPVMPGDLPF"
     gene            complement(731..>1592)
                     /locus_tag="PLO59_07630"
     CDS             complement(731..>1592)
                     /locus_tag="PLO59_07630"
                     /inference="COORDINATES: protein motif:HMM:NF026165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HQV01014.1"
                     /translation="IYIKNQILKLWQGLGYYSRARNMHEAAKQLIHNGGVFPADYNAL
                     LKIKGIGSYTAAAIASFAFNLPHAVVDGNVYRVLSRYFGIGTPINSAVGKKQFAELAQ
                     RMLPGQNSATYNQAIMELGALICKPEQANCKHCPLQLGCYANATKQLNLLPVKTAAKP
                     VKKRYMYFVLFHDNERLYLKQRTDKDIWQNLYVLPYIETATQQPIDKILESKFLKEFM
                     EQHDVTVTVHEKTITHKLTHQHLITQFVNCRVKKLPANNNYKLSNSRQLNNYAVPRLI
                     EKYLTEQGWL"
BASE COUNT          457 a          316 c          286 g          533 t
ORIGIN      
        1 aagacactgt ttaaattagg cagtgtcttt tttttattcg ctaatgcggt aagcgtaatg
       61 tcatctaaat atcccacata aaactctaaa aatgttttcg aaaacaacag agcttacatc
      121 catacccggc aatttgcaag accgtaaaat taaatggcat gcgcaatgca taaaaaacca
      181 cctctacaat agttgcctgt taaattaaaa cggcaaatcg cccggcatta caggtccatc
      241 gggcacaggt gcgctcttta cttctgttgc acttccgttt tcaacaatgg caggtcgttt
      301 aataaccgtt atcgaatcag cattaatttc tgtatatgat tttttgtgcc cctctttatc
      361 atcataagta cggttgcgaa ttttgccttc aacataaagc atggtgccct tttttaattg
      421 cagtttttca atgctttcgg ccaaagcacg ccatgctaca atggtatgcc attcggtttg
      481 ctcttgttta atgccgtctt tgtttttata gttttcagtt gtggctaaag gaaacgttgc
      541 cttagcgtag ccgccttcca ggtgtttaat ttcgggttct ttgcccagat tgccgattag
      601 taaaacttta ttgagtcctg acatgttatg gttaatttag gtgtttggca aatataaaag
      661 tgcagcccaa ttatcaaagt accaaatagg ttaacggata ttgcaaatgg gcaaaatcaa
      721 atttatgccg ttatagccag ccttgctccg tcaaatactt ctctatcaaa cgcggcacag
      781 catagttgtt aagctgccgg ctgttgctta acttatagtt attatttgca ggcaattttt
      841 ttaccctgca atttacaaat tgtgttatca gatgttggtg ggttaactta tgagttatgg
      901 ttttctcatg tacggtaaca gtaacatcat gttgctccat aaattctttt aagaatttcg
      961 attctaaaat tttatctatt ggctgttggg tagctgtttc aatgtatggt aacacataca
     1021 aattttgcca aatatcttta tcggttcttt gcttcaaata aagacgttca ttgtcatgaa
     1081 acaatacaaa atacatgtag cgtttcttaa cgggttttgc agcagtttta acaggcaata
     1141 aatttaactg ctttgttgca ttggcataac agcccaattg caacggacag tgtttgcagt
     1201 tagcttgctc gggtttacat attaatgcac ccaactccat tattgcctga ttgtaggtag
     1261 cactattttg gccgggcaac attctttgtg ctaactcagc aaattgcttt ttgcccactg
     1321 cactatttat aggcgttcct attccaaaat aacgcgacaa tactctgtaa acgttaccat
     1381 caactacagc atgtggcaaa ttaaatgcaa acgaagcaat agccgctgca gtgtaagagc
     1441 caataccttt aattttaagt aaggcattgt aatcggccgg aaaaacgcca ccattatgaa
     1501 ttaattgctt agctgcttca tgcatattac gcgcacgcga ataataacct aagccttgcc
     1561 ataattttaa aatttgattt ttaatataaa ta
//
LOCUS       DAOMAA010000558         1592 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_88011_length_1592_cov_1.983084, whole genome shotgun sequence.
ACCESSION   DAOMAA010000558 DAOMAA010000000
VERSION     DAOMAA010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88011_length_1592_cov_1.983084"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="PLO59_07635"
     CDS             <1..312
                     /locus_tag="PLO59_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01015.1"
                     /translation="IVPIIDTTVVVVNRVSRGQSPFVGGRDHTTHSLAYLGLSDRQVA
                     YIFAGISANSIFLITYIKRYIITWSWEFTLLFGGYFIILLSVFFYFAFKAGQSAIQKA
                     K"
     gene            complement(313..747)
                     /locus_tag="PLO59_07640"
     CDS             complement(313..747)
                     /locus_tag="PLO59_07640"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013631981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HQV01016.1"
                     /translation="MNLHIINGPNLNLLGIRQPELYGSRTFESYFLQLQNQFKNHSIY
                     YFQSNVEGILIDKLHEIGFNSDGIIINAGGYTHTSVAIGDALASITAPVIEVHISNIY
                     AREPFRHQSYLSRHCKAVIAGAGLSGYSFALQLLTQQNDFSK"
     gene            937..1404
                     /locus_tag="PLO59_07645"
     CDS             937..1404
                     /locus_tag="PLO59_07645"
                     /inference="COORDINATES: protein motif:HMM:NF014898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HQV01017.1"
                     /translation="MWQSYIKGFESYLLLERSLSKNTLEAYADDLNKLLQFLNFKEIN
                     IPPEQITLSHLQDFIAWIHSLGMSAHSQARIISGIKAFYKYLMVENIIQKNPTRLLQS
                     PKLGRKLPDVLSVDEIETILAVIDLSKPDGERNKAIIETLYSCGLRVSELVSL"
BASE COUNT          505 a          277 c          279 g          531 t
ORIGIN      
        1 attgttccta taattgatac aactgtagtt gtagttaatc gcgtgtcgcg tgggcaatca
       61 ccttttgttg gtggccgcga tcatacaaca catagcctgg cttacttagg tttatccgac
      121 cgccaggttg cttatatttt tgcaggcata agtgcaaaca gtatttttct gattacctac
      181 attaaacgtt atattataac ctggagttgg gagtttacct tattgtttgg tggttatttt
      241 attatactgc tatccgtgtt tttttacttt gcatttaaag caggccaatc agcaattcaa
      301 aaagcaaaat agttatttgc taaaatcatt ttgttgcgtt aaaagttgca gtgcaaaact
      361 atatcctgat aaacccgcac ctgcgattac tgctttacaa tgccggctta aataagattg
      421 atgtctaaac ggttcgcgtg cataaatgtt cgaaatatgt acttcaataa ctggtgctgt
      481 aatagatgcc aatgcatcgc caatggcaac actcgtatgt gtatatccgc cagcatttat
      541 aataatacca tcagagttaa atccaatttc atgcaacttg tcaataagga tgccttcaac
      601 attactttga aaataatata tggaatggtt tttaaattga ttttgcaatt gtaaaaaata
      661 gctttcgaaa gttcgactgc cgtacaattc cggctgcctg atgcctaata aatttaagtt
      721 agggccgttt ataatatgaa ggttcatttt aaatgggcta ttaaatacat tggccaaata
      781 tatttgataa ttaacaaaaa tattttctga atttctttta cagtaaaata atgtttctat
      841 tattgtgcac attgattatt acccctttaa tcaacaaacc acttaggcat ttgccaagtg
      901 gtttttttat gtaacaaccg gtaacttttc ggcttcatgt ggcagtcata tataaaaggt
      961 ttcgagtcct acttgttatt agaaaggtcg ctttcgaaaa atacattaga ggcctatgcc
     1021 gatgatttaa ataagttgtt acaattttta aattttaaag aaattaatat cccgcccgag
     1081 cagataaccc tttctcattt acaggatttt attgcttgga tacatagctt aggcatgagt
     1141 gcacattcgc aagcacgtat tatttccggc atcaaagctt tttataaata tttgatggta
     1201 gaaaatatca tccaaaaaaa ccctacgcgc ttattacaat cgcccaagct tggccgtaaa
     1261 ttgcctgatg tattatcggt agatgaaata gaaacaatat tggctgtaat tgacctgagc
     1321 aaacctgatg gcgaacgtaa taaggctatt atagaaacct tatatagttg cggcttgcgg
     1381 gttagcgaac tggtttcact ttaaatatcc gacatttatt tcgaaattgg atttattaaa
     1441 gttataggca agggcaataa ataacggctg gtacctatcg gacaaattgc tatcagacaa
     1501 ttaaaaaatt attttgaaaa aacacgcaat catcaaacaa ttgctacaag ccattctgac
     1561 tatctgtttc ttaacagagg gggcaaaaaa at
//
LOCUS       DAOMAA010000559         1591 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_88085_length_1591_cov_2.580078, whole genome shotgun sequence.
ACCESSION   DAOMAA010000559 DAOMAA010000000
VERSION     DAOMAA010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88085_length_1591_cov_2.580078"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="PLO59_07650"
     CDS             <1..244
                     /locus_tag="PLO59_07650"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HQV01018.1"
                     /translation="QAMDNYVVIQTSADQFVVHSTMRNIEAKLPAENFVRVHRSYIVQ
                     TDKITSMEDNSIVINDKIIPVGKSYRDLFLKRFNFL"
     gene            complement(256..>1591)
                     /locus_tag="PLO59_07655"
     CDS             complement(256..>1591)
                     /locus_tag="PLO59_07655"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HQV01019.1"
                     /translation="PTPTANLSVPINQVCLGLPITVTATSGNNVFYSFNMGDGVTINN
                     NPPVNYVYSDTGMYAVTLTVTDGNNCSFDTTFNSVFIRPIAIADFTFTQVPLCAMPAQ
                     INFINQSVSAGSYQWYFGVTDSSQQTNPVRTYNNDTSFTTTLIANNLYGCKSSSDTLI
                     NVYAAPVTNFSASDTVICPGDVVTFTQNSSSNANTFYWNFGNGTTSTLAAPPPQQYNN
                     SGIYSVWLIAGNNGICFDTLYRLHYINVQAKPQAQFSYLSVDTVVNQIMFSPSGYYQF
                     KDLSINANTVFWDFDDGTNSIFHNPLHLYYSSGERCVMLIARNNNECSDTAVTCFLVD
                     LPGTLYLPNSVSPDNGNDEERSFFAKGTSIQNYKLEIFSPFGELVWFCEETTFENGIS
                     KCKWQGTDMNGKGLPQGAYVWKVSGTFENGSKLIDMSEKGKLRNTGTVMLLR"
BASE COUNT          484 a          302 c          292 g          513 t
ORIGIN      
        1 acaagccatg gataattacg tagttattca aaccagtgcc gatcagtttg ttgtacattc
       61 aaccatgcgc aatattgaag caaaattacc agccgaaaat tttgtgcgcg tgcaccgttc
      121 ttacattgtg caaaccgata aaattacttc aatggaagac aattcaattg tgataaatga
      181 taagattata ccggtaggta aatcgtatcg tgatttgttt ttaaaacggt tcaacttttt
      241 atagcgaagc ctatcttatc gtaaaagcat tacggttccg gtatttctta attttccttt
      301 ttcgctcatg tcaataagtt tgcttccgtt ttcaaacgta cccgaaactt tccatacata
      361 agcaccttgc ggtaatccct tgccattcat atcagttcct tgccatttgc attttgatat
      421 gccgttttca aaagtggttt cttcacaaaa ccaaactaac tcgccaaatg gcgaaaatat
      481 ttctaactta taattttgta tcgaagttcc tttcgcaaaa aagctacgtt cttcgtcatt
      541 gccgttatca ggcgatacac tgttgggcaa gtataaagtg cctggcaagt caactaaaaa
      601 acatgttact gcggtatcgc tacattcatt attatttctg gcaataagca taacgcatct
      661 ttcgccactg ctataatata aatgcaatgg gttatgaaat atactgttgg tgccatcatc
      721 aaaatcccaa aaaacggtat ttgcattaat gcttaagtct ttaaactgat aataaccgct
      781 tggcgaaaac ataatctgat taacaactgt atcaacactt agatatgaaa attgtgcctg
      841 tggtttagct tgcacgttaa tataatgcaa tcggtataaa gtgtcgaaac aaataccatt
      901 gttgccggca ataagccaca cactatatat gccactgttg ttatattgtt gtggtggtgg
      961 cgctgctagg gtagatgttg ttccattacc aaaattccaa taaaatgtat ttgcattgct
     1021 gctgctgttt tgtgtaaaag taactacatc gccagggcat attaccgtat cgctagcgct
     1081 aaaatttgta actggtgcag catatacatt aatcaacgta tcgctgctgc ttttacatcc
     1141 atataaatta tttgcaatta gcgtagttgt aaatgatgta tcgttattat aagtacgtac
     1201 cggattggtt tgttgtgagc tatctgtaac tccaaaatac cattggtaac taccggctga
     1261 tacactttgg tttataaaat taatttgtgc aggcatggca cacaagggta cttgtgtaaa
     1321 cgtaaaatca gcaatggcaa tggggcgtat aaacacactg ttaaaggttg tatcgaaact
     1381 acaattatta ccatcggtaa cggtaagggt tacagcatac atacctgtat cgctataaac
     1441 ataatttaca ggcgggttgt tatttatggt tacaccatcg cccatattaa aactgtaaaa
     1501 cacattgttg ccagaggttg ccgttaccgt tatgggcaag cctaaacata cttgatttat
     1561 aggtactgat aagtttgctg ttggcgtagg c
//
LOCUS       DAOMAA010000560         1591 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_88104_length_1591_cov_2.325521, whole genome shotgun sequence.
ACCESSION   DAOMAA010000560 DAOMAA010000000
VERSION     DAOMAA010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88104_length_1591_cov_2.325521"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(181..1137)
                     /locus_tag="PLO59_07660"
     CDS             complement(181..1137)
                     /locus_tag="PLO59_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HQV01020.1"
                     /translation="MQTYTILDKKDTRSGFGAGLTYLGKTNKNVVALCADLTGSLKMD
                     AFAKAHPDRFFQVGIAEANMMGIAAGLTIGGKIPFTGTFANFSTGRVYDQIRQSIAYS
                     GKNVKICASHAGLTLGEDGATHQILEDVGMMRMLPGMVVINPCDYNQTMAATIAIAQH
                     QGPVYLRFGRPVVPNFTSPDQIFEIGKAVTLNTGADVSIFATGHLVWKAIEAGHILAQ
                     QGIDAEIINIHTIKPLDVNTVIQSVKKTGCAVTAEEHQLNGGLGDAIAQTLVCNLPRP
                     IEMVGVNDSFGESGKPEELLEKYGLHAQAIVNAALKSIKRKM"
     gene            complement(1169..>1591)
                     /locus_tag="PLO59_07665"
     CDS             complement(1169..>1591)
                     /locus_tag="PLO59_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01021.1"
                     /translation="KNLKCIVRLKGKNETLHVQDFNTKERYLIGDYDLEVLCLPRFKI
                     NDVVISQSHTTTVQIPNPGIATMLLNTVGYGCIVQEDKDELKWVVNLDALQMRQTFVL
                     QPGNYRVLFRSKNAVEAEYSIEKSFKVASGSSVSVTIN"
BASE COUNT          430 a          316 c          297 g          548 t
ORIGIN      
        1 atcgtcatgg tcaataacca atctgcgtat gtgatggtat attttaattt gatatttctc
       61 gacaatgtgc ttgaaggcat catggctggt tgtttggttt ttaaacaact ccagcaattg
      121 tgcatcagaa taatggttta tactcatctt gcaccgtaag tattaaagat tgttgagttt
      181 ttacatttta cgcttaatac ttttaagtgc agcattaaca atagcttgtg catgtaaacc
      241 atatttttcg agcagttctt cgggtttgcc actttcgcca aacgaatcgt ttactcccac
      301 catttcgata gggcggggta ggttgcaaac caatgtttgt gctatggcat cgcctaaacc
      361 tccatttagt tgatgttctt cggctgtaac tgcacagcct gtttttttta cagattgtat
      421 gactgtatta acatctaatg gtttaatggt gtgtatatta attatctcgg catctatacc
      481 ttgttgggct aaaatatggc cggcttcaat tgctttccat actaaatggc ctgttgcaaa
      541 aatagaaaca tcagcgcctg tattaagtgt tacggcttta cctatttcaa aaatttgatc
      601 cgggcttgta aagttaggta ctaccggcct gccaaagcgc aaataaacag ggccttgatg
      661 ttgtgcaata gcaattgtgg ctgccatagt ttggttataa tcgcatggat taattactac
      721 catgcccggc aacattcgca tcatacctac gtcttccaat atctgatgtg tggcaccatc
      781 ttcgccaagg gttaaaccgg catgtgaggc acatattttt acgtttttac ccgagtaagc
      841 aattgattga cgtatttgat cataaacacg gcctgtcgaa aaattggcaa atgtgcccgt
      901 aaaaggaatt ttgccaccta tagttaaacc ggctgcaata cccatcatat tggcttcggc
      961 tatacctacc tgaaaaaacc tgtcgggatg ggctttggca aatgcatcca tttttaatga
     1021 tccggttaaa tcggcacata gtgcaaccac atttttattg gttttaccta agtatgttaa
     1081 accggctcca aagcctgagc gcgtatcttt tttatcaagt atggtatagg tttgcatttt
     1141 acttgttcga atatgtattg gtgtttaatt aatttattgt taccgacacc gaactgcccg
     1201 atgccacttt aaatgatttt tcgatgctgt attcggcctc aacagcattt ttgctcctaa
     1261 atagtactct ataattgccg ggttgcaata caaatgtttg gcgcatttgt aaggcatcta
     1321 aatttacaac ccattttaat tcatctttat cttcctgtac aatacagcca taacctactg
     1381 tatttaaaag catggttgca atacccggat tgggtatttg caccgtggtt gtatgacttt
     1441 gcgaaattac tacatcatta attttaaagc gaggcaggca taaaacttct aagtcgtaat
     1501 cgccaatcaa ataccgctct tttgtattaa agtcttgtac atgtaaggtt tcattttttc
     1561 cttttaacct tacaatacac tttaagtttt t
//
LOCUS       DAOMAA010000561         1585 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_88739_length_1585_cov_2.822876, whole genome shotgun sequence.
ACCESSION   DAOMAA010000561 DAOMAA010000000
VERSION     DAOMAA010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88739_length_1585_cov_2.822876"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            175..1287
                     /locus_tag="PLO59_07670"
     CDS             175..1287
                     /locus_tag="PLO59_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01022.1"
                     /translation="MKKTFTFLIAWLCVYSLVMTTHAQNLYAYQAYNNNLIIFDNGNF
                     VTAETLPVLKKFIGFDYVAYISNNNLLKMYWRKQTNEINEAAFITRSEGKRGLFAYQM
                     DKQLKVVYNGKDITLTTFCNNWNASDSLVLFYDDVRNMHFVFYNGDVFALDDVLQGKT
                     GLFNGSNCSIGGNILCYVNSFNKLRAQYNGVFYNLSDYNRFLAFEAGNNICVYLNENS
                     NAWTAFCNGYTNVLDQFEPQSYQLGNGMVAYVDNLQNFKVFYNDTIIRLLPVQPAGYQ
                     VTDSLISFSDNVNNWYVFYNGRMQKLENYIPTQIKMSNATLCYIDNVGALRVFIRGKS
                     QVITTEQVRQFEVTNNVVSYTLGNSDVRIYWDGNKY"
     gene            1399..1557
                     /locus_tag="PLO59_07675"
                     /pseudo
     CDS             1399..1557
                     /locus_tag="PLO59_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009187054.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          556 a          263 c          286 g          480 t
ORIGIN      
        1 ataaatgcag gccaaccggc tttagataat ttacaatttt atgttgaaac agaagctgaa
       61 aaaaatcaac gcgaagccga agataaaatg tggcgcaccg gtaaagcacc cgccaacatt
      121 aaaggccatg atgattagga atttttcctg tttaattctt taaaataaac gaacttgaaa
      181 aaaacattta catttctaat agcttggctt tgtgtttatt cattagtaat gactacccat
      241 gcgcaaaatt tgtacgccta tcaggcttat aacaacaacc tgataatatt tgataacgga
      301 aactttgtta ctgccgaaac tttaccggtt ttaaaaaaat ttattggctt tgattatgtt
      361 gcctacataa gtaataacaa tttgctaaaa atgtattggc gcaaacaaac caatgaaata
      421 aatgaagctg cttttattac tcgtagtgaa ggtaaacgtg gcttgtttgc atatcaaatg
      481 gataagcaat taaaagttgt atataatggc aaagatataa cgctaaccac attttgtaat
      541 aactggaatg cctctgatag tttggttttg ttttatgatg atgtgcgtaa tatgcatttc
      601 gttttttata acggggatgt atttgcatta gatgatgtat tacaaggcaa aaccggtttg
      661 tttaacggca gtaattgttc gatagggggc aatatacttt gctatgtaaa ttcatttaat
      721 aaactgcgtg cacaatacaa tggcgtgttt tataatttat cagactacaa ccgtttttta
      781 gcgttcgaag caggtaataa tatttgtgtt tatttaaatg agaacagcaa tgcatggaca
      841 gcattttgca atggttacac caatgtatta gatcagtttg aaccccaatc ataccaatta
      901 ggcaacggca tggttgctta tgtagataac ctgcaaaatt ttaaagtttt ttataacgat
      961 accattatac gtttactacc tgtacaaccg gccggttatc aggttaccga tagtttgata
     1021 agctttagtg acaatgtaaa caactggtat gttttttata atggacgcat gcagaaatta
     1081 gaaaactaca taccaactca gataaaaatg agcaatgcaa cgctttgcta tattgataat
     1141 gttggcgcat tgcgggtatt tatccgcggc aaaagtcagg taattacaac agaacaagtg
     1201 cgtcaatttg aagtgaccaa taatgtagta tcatacactt tgggtaattc agatgttcgc
     1261 atttattggg atggtaataa gtattagtta tataccacgc aaccatgcac atgtaactaa
     1321 atgtagcttg tggaattaat caagcaaaac ataacaggaa aaacaccatt attaaagata
     1381 ccaccttgca taccatgcta taggtatgcc acacacttac ttgaggccgg aaccgatatg
     1441 cgctacatac aggaactgtt ggaacacaac gcacccaaaa ccacgatgat ctatacacaa
     1501 gtgagtacta aaaagctaag cgaaataaaa agcccattag atgatttaga tgtttaaaat
     1561 taacctgaac caatcaaaat ataaa
//
LOCUS       DAOMAA010000562         1584 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_88822_length_1584_cov_3.250491, whole genome shotgun sequence.
ACCESSION   DAOMAA010000562 DAOMAA010000000
VERSION     DAOMAA010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88822_length_1584_cov_3.250491"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1584
                     /locus_tag="PLO59_07680"
     CDS             <1..>1584
                     /locus_tag="PLO59_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01023.1"
                     /translation="NQKIRLPDNFALLETCSIKGKFTGFLTDFVAYGNVHTALGFIST
                     DINLKTGQQRETYSGHVSTNQFDIGKFIGQSQLLGKATVNCNIQGQGFKFDNLVASIK
                     GDVLAINFNNYNYSNINVQGELARKKFKGALDIDDKNLDLQFAGSVDYTKAEPIFIGN
                     AVIAQGYLSKLNLVDRDTSAVVSGNLNFNLSGDDIDNMYGALNISELHYSEAGKHIYL
                     DDLSFDAFDDKNKKHFLLTSNIVDADIVGISRLSTLARSIQNVVENYFPVAATKRSVA
                     VKPEIGNFKISIKDANPTLAIFYPDLTIASGTTIEGSINAFNAQIKMVAISPQVKHNL
                     LGFENLMVSAESANQKLLMKLSSEKFIFDDSLHLKALNTHLTSTRDSASLTLIMATPD
                     STSINLNVTANAAFYTNNTSIQIDSSSKILLRNNAWHINPQNTIHLFEGVRFNNFFLS
                     NNKNQQLNLEGLIGSPNFNDKLKLTFQQFDISLLNPILKTYDVALGGIVNGDILYSNT
                     NNKLDIGSNLSIDTLSVFND"
BASE COUNT          569 a          273 c          268 g          474 t
ORIGIN      
        1 aatcaaaaaa ttaggctgcc cgataatttt gccttgctag aaacctgttc gattaaagga
       61 aaatttacag gctttttaac cgactttgta gcatatggta atgtgcacac agcgttaggc
      121 tttatttcta cagatataaa tttaaaaacc ggccagcaac gcgaaactta ctcgggccac
      181 gtttcaacca accaatttga tatcggcaaa tttattggcc aaagccaact acttggtaaa
      241 gctactgtta actgtaatat acaaggtcag ggttttaaat tcgataattt agttgcaagt
      301 ataaaaggcg atgtattggc aattaatttt aataattata actattcaaa tattaacgta
      361 caaggcgagt tggcacgcaa aaagtttaaa ggtgcattag atattgatga taaaaactta
      421 gacctacaat ttgccggaag cgttgattac accaaagccg aaccaatttt tattggtaat
      481 gcagttatag cccaaggcta tttaagcaaa ttaaatttag ttgacagaga tacaagcgca
      541 gttgtatcgg gcaatttaaa ttttaatttg agtggcgatg atattgataa tatgtatgga
      601 gccctaaaca ttagcgaact gcattatagc gaagcaggaa agcacatata tttagatgac
      661 ttaagttttg atgcatttga cgacaaaaac aaaaagcact ttttacttac ttcaaatatt
      721 gtagatgcag acatagttgg tatcagtcgt ttaagtacac tggcacgctc tattcaaaat
      781 gtggtcgaaa attattttcc ggttgcagca accaaaagaa gtgttgccgt aaaacctgaa
      841 atcggtaatt ttaaaataag tattaaagat gccaatccaa cattagccat attttatccc
      901 gatttaacta ttgcctccgg cacaacaatc gaaggaagta ttaatgcatt taatgcacag
      961 attaaaatgg ttgcaataag cccgcaggta aaacataact tattagggtt cgaaaactta
     1021 atggtaagtg ccgaatcggc taaccaaaaa ctacttatga aattaagttc cgaaaaattt
     1081 atttttgatg atagcctgca tctaaaagca ctaaacacac atctcacctc aacacgcgat
     1141 tcggcaagcc ttacattaat tatggcaaca cccgatagta ccagtattaa ccttaatgtt
     1201 acagcaaatg ctgcattcta cacaaacaat accagcatac aaattgatag cagcagtaaa
     1261 attttattgc gcaacaatgc ttggcatatc aatccgcaaa acacgataca cttatttgaa
     1321 ggagttaggt ttaataattt ctttttaagc aataataaaa atcaacaact aaacttagaa
     1381 ggattaattg gcagcccaaa ttttaatgat aagcttaaac ttacgtttca acaatttgat
     1441 ataagtttgc ttaaccccat tttaaaaact tacgatgttg ctttaggcgg tattgttaat
     1501 ggagatatac tatacagcaa caccaacaac aaattagata ttggtagcaa tttaagtatt
     1561 gatactttgt cggtttttaa tgat
//
LOCUS       DAOMAA010000563         1581 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_89155_length_1581_cov_3.465269, whole genome shotgun sequence.
ACCESSION   DAOMAA010000563 DAOMAA010000000
VERSION     DAOMAA010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89155_length_1581_cov_3.465269"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1008
                     /locus_tag="PLO59_07685"
     CDS             <1..1008
                     /locus_tag="PLO59_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011586628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HQV01024.1"
                     /translation="STDFDIADKLYFEPVFWEHIYDIILHEKPEGVIVQLGGQTALKL
                     AEKLDKYGIKIIGTSYQSLDLAEDRGSFSTLLKQLEIPYPKFGVVEDADQAITLSRQL
                     GFPLLVRPSYVLGGQSMKIVINEQELEQHVVNLLYDLPGNKVLLDHFLDKAIEAEADA
                     ICDGNNSYIIGIMEHIEPSGIHSGDSNAVLPPFDLSADVINQIENITKRIAKAMQVKG
                     LINIQFAIKNEVVYVIEANPRASRTVPFIAKAYDEPYVNYATKLMLGTHTLADFNFNP
                     RKQGYAIKIPVFSFEKFQDVNKELGPEMKSTGEAIYFIDDLEDEFFQKVYSERNLYLS
                     K"
BASE COUNT          544 a          295 c          286 g          456 t
ORIGIN      
        1 agtaccgatt ttgatattgc cgataaattg tatttcgaac ctgtgttttg ggaacatatt
       61 tacgatatta tccttcatga aaaacccgaa ggcgtaattg tgcaattagg aggacaaacc
      121 gcattaaagc ttgccgaaaa attggataag tatggcatta agataattgg caccagttac
      181 caatcgctcg accttgccga agaccgtggc agctttagta cactgctaaa acaattagaa
      241 ataccctacc cgaaatttgg tgtagtagaa gatgccgatc aggccattac cttatcacgc
      301 caattgggct ttccactatt ggtaaggccc agttatgttt tgggtggcca aagcatgaaa
      361 attgtaataa acgaacaaga acttgaacaa catgtggtaa acctgcttta tgatttgccg
      421 ggcaataaag tattgctcga ccatttttta gataaagcca tcgaagccga agctgatgct
      481 atttgtgatg gaaacaacag ttacataatc ggcattatgg aacatattga accgtcaggc
      541 atacacagtg gcgatagcaa tgcagtattg ccaccctttg atttaagtgc tgacgtaata
      601 aatcaaattg agaatattac caagcgcatt gccaaagcca tgcaggtaaa aggattgata
      661 aatatacagt ttgccattaa aaacgaagta gtgtatgtta tcgaagccaa tccgcgtgct
      721 tcgcgcacag tgccttttat tgccaaagct tatgacgagc cttatgtaaa ctatgctact
      781 aaactgatgt taggtacaca tacacttgcc gattttaatt ttaatccccg caagcaaggt
      841 tatgctatta aaataccggt atttagtttc gaaaaatttc aggacgtaaa caaagaatta
      901 ggccccgaaa tgaaatctac aggcgaagct atttatttta ttgatgacct ggaagacgag
      961 ttttttcaga aagtatatag cgaacgtaat ttatatttaa gtaagtaaag gtttttgtaa
     1021 ttgttattga agtagcattt tattattggt taaaatgata gttaagggtt aacagaacaa
     1081 ctgaaaaacg attataatcc cactacccta aacttgccac tgcctgccgg tgaccggatg
     1141 tagcagcctg agcatttaaa tatttttgct tgtgactatt gtaggaataa aaacacgaaa
     1201 atcaatcctg taatcttaaa atcctacaaa tcatagttca aacaatttta ctaaccacaa
     1261 ccttaaacgc agtataaacc cgttactttt ttacaacttt tttaatagca atattttggc
     1321 tatcaattac ctttaatatg taggggcctg ctggcattaa ggcaattggt acttcaaaaa
     1381 cgtgcatgcc gttttttacc atttgcaatt agaaacaaaa gataaacgaa gaggcggtga
     1441 gaaggtttat aaaacaataa acccttactg cccacaaatc aacgcactcc ctaaagggaa
     1501 agaaaaatca ggacttaaaa atcctataat cttaaaatcc tacaaatcat agttcaaaca
     1561 attttactaa ccacaacctt a
//
LOCUS       DAOMAA010000564         1580 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_89250_length_1580_cov_3.672787, whole genome shotgun sequence.
ACCESSION   DAOMAA010000564 DAOMAA010000000
VERSION     DAOMAA010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89250_length_1580_cov_3.672787"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1253
                     /locus_tag="PLO59_07690"
     CDS             <1..1253
                     /locus_tag="PLO59_07690"
                     /inference="COORDINATES: protein motif:HMM:NF015657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01025.1"
                     /translation="IIYKNCSNCHRPNQSGPFSLLSYSDCRNKAATIKYTVETRFMPP
                     WPADYTYQHYIGEKYLTVAEINRICNWVDDGALMGDSDKIPAPPHFYAHSMLGKPDLV
                     VKATPFFIKGDKHERFIMMKIPFENVADTFIRAIEFVTNNKKLVHHVNGHLLNYLPHQ
                     TTNLYKPPFVIDRDTAYEIAESFHNLNLTANDGSEPAFIGSVTNYLPGMETLIFPDGI
                     GTYKVNKHAALLLRDIHYGATTVDTWDASYFNLFYADKPPKRPTIERQLGTLGISQIT
                     PPLVVRPNKIDTFRTQATLPVDISLLTINPHMHLIGKSFWAYAVKPNGDTIPIIRIPK
                     WDFRWQYFYTFKTMLKIPKGATIYVVGVYDNTANNPNNPFYPPREIREPVGGNMKTTD
                     EMFQLIMTYLPYQKGDENISLQNP"
     gene            1270..>1580
                     /locus_tag="PLO59_07695"
     CDS             1270..>1580
                     /locus_tag="PLO59_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002693603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQV01026.1"
                     /translation="MRLLFFYSCIMLLFNACTEKKKADAIFYNAVVYTVDSTFSVAEA
                     FAVADGKIIAIGSKADILKYDATNKIDLQGRFVYPGFADAHCHFYGYGVDLKLINLTG
                     T"
BASE COUNT          506 a          325 c          269 g          480 t
ORIGIN      
        1 caataattta taagaactgc agcaattgcc accggcccaa tcaaagtggc ccgttttcat
       61 tattaagtta cagcgattgc cgtaacaaag cagccactat taaatatact gtagaaaccc
      121 gctttatgcc accctggcct gctgattata cgtaccaaca ttacattggc gaaaagtatt
      181 taacggttgc tgaaataaac cgtatttgta actgggttga tgatggcgct ttaatgggcg
      241 attcggataa gataccggca ccacctcatt tttatgccca ctctatgttg ggcaagcccg
      301 atttggttgt aaaagcaacg ccatttttta ttaaaggcga taaacatgag cgttttataa
      361 tgatgaaaat accattcgaa aatgttgccg atacttttat aagagccatt gaatttgtaa
      421 ccaacaataa aaagttagta catcatgtaa atggtcattt attaaattat ttaccccatc
      481 aaactactaa tttatacaag cccccatttg taattgacag agatacggct tacgaaattg
      541 ccgagtcgtt tcacaactta aacttaactg ctaacgatgg cagcgaacct gcatttatag
      601 gaagtgttac aaactactta cccggtatgg aaacattaat ttttccggat ggtataggca
      661 catataaagt aaacaaacat gcagcacttt tattacgcga tattcattac ggtgccacca
      721 cagttgatac ctgggacgcg tcatacttca atctttttta tgctgataaa cctcccaaac
      781 gtcctactat cgaacggcag ttaggcacct taggcatttc gcaaattaca cctccgttag
      841 tagtacgccc caataaaatt gacacgttta gaacacaagc taccttaccg gttgatattt
      901 cgttattaac tattaatcca cacatgcatt taataggtaa atcgttttgg gcttatgctg
      961 ttaaacccaa tggcgatacc atacctatta tacgcatacc caaatgggat ttcagatggc
     1021 aatactttta tacttttaaa accatgctta aaatacctaa aggagctacc atttatgtag
     1081 taggcgttta tgataatacg gccaataatc cgaacaatcc cttttaccca ccacgcgaaa
     1141 tacgcgaacc ggttggcggc aatatgaaaa ccactgacga aatgtttcag ttgattatga
     1201 cttacctacc ctatcaaaaa ggcgatgaaa atataagcct acaaaaccct taaaaacact
     1261 tacactttta tgagattatt atttttttac agttgtataa tgcttctttt taatgcatgc
     1321 actgaaaaga aaaaagccga tgcaattttt tacaatgctg ttgtttacac cgttgacagc
     1381 acattttctg ttgccgaagc ttttgcagtt gccgatggaa aaattattgc cattggtagc
     1441 aaagccgata ttttaaaata tgatgctaca aataaaattg atttacaagg ccgttttgtt
     1501 tatccgggat ttgccgatgc gcattgtcac ttctatggtt atggtgttga cttaaaactt
     1561 ataaacttaa ccggtacaca
//
LOCUS       DAOMAA010000565         1580 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_89292_length_1580_cov_2.606557, whole genome shotgun sequence.
ACCESSION   DAOMAA010000565 DAOMAA010000000
VERSION     DAOMAA010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89292_length_1580_cov_2.606557"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1580)
                     /locus_tag="PLO59_07700"
     CDS             complement(<1..>1580)
                     /locus_tag="PLO59_07700"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HQV01027.1"
                     /translation="DKLRNLIWIGYNNGGMQLFDVRTRKLNGLYNVSGNSTYDIFLSD
                     TMAYVATIVNSLKKVSSPNAFKKHKDITQAAITINCIVNASNGRLYCGAWDNALHEFD
                     YNTKLIKSYIFDGKDTLNFSADEIISIAEDENGILWCGTKNAGVLFFDLKTKTFTGTY
                     KYVYNITTRVNCIYRDDYNRMWIGSQQGIFVYDRLMNQFEQTYFYNNIDKINCKVHQR
                     LLVTGGTEYVVADCGLFYKKVNEVDYTFKNFRYEHDDLQLTSIYCDNAGTIFIGSNKT
                     IFTLDTLKMELSIIPVAKQSVTQGFLSAYASRINSITGLQLDNKNYVINSFYGHFVSI
                     VDLERKNYFMLKRTKPNNIENLVRKIYIDSKNRIWICGASKGLSQFYLPVDFHPDSFP
                     VCDTLKHPVIITSQTWENEKSKTPFAINNVYDILENKDGSFWLTSQGNGLFKFYPDNK
                     SVPFIPINGDYKSLQGIARGNANTVWIITSKGVLSYNTQTAQYKLYDAAHGIPESTAG
                     YFFNENDSTLCAGFNGGF"
BASE COUNT          495 a          301 c          255 g          529 t
ORIGIN      
        1 taaaaccacc attaaaacct gcacaaagtg tactgtcgtt ttcattaaaa aaatagcctg
       61 cagtactttc aggtattcca tgtgctgcat catacaattt gtattgcgct gtttgtgtat
      121 tgtaacttaa cacaccctta gatgttatta tccaaacggt attggcatta cccctggcaa
      181 tgccctgcaa cgatttgtaa tcgccattaa tgggtataaa cgggaccgat ttattatcgg
      241 gataaaattt aaacaggcca ttaccttgcg aagtcaacca aaagctgcca tctttatttt
      301 cgagtatatc atatacgtta ttaatggcaa agggtgtttt gcttttctca ttttcccatg
      361 tttgtgaagt aataattacc ggatgtttca acgtatcaca caccggaaac gaatcgggat
      421 gaaaatcaac aggcaaataa aattgcgaca aaccttttga ggcgccacaa atccaaatac
      481 gattttttga gtcaatataa attttgcgta ccaggttctc gatgttgttt ggttttgtgc
      541 gcttaagcat gaaatagttt ttccgttcta aatctacgat gctaacaaag tgcccataaa
      601 aggaatttat cacataattt ttattatcta attgcaaacc tgtaatcgaa tttatacgcg
      661 aagcataagc cgataaaaaa ccttgcgtta cactttgctt tgcaacaggt attatagaca
      721 attccatttt caatgtatcc aaagtaaaaa tggttttatt gcttccaata aatattgtgc
      781 ctgcattatc gcaatagata cttgttaact gtaagtcatc atgctcgtat ctgaaatttt
      841 taaatgtgta atcaacttcg tttacttttt tataaaataa accacagtca gccaccacat
      901 attcagtacc accagttact aataaacgtt gatgcacttt gcaatttatt ttatcaatgt
      961 tgttatagaa ataggtttgc tcaaactgat tcatcaacct gtcatacaca aaaatacctt
     1021 gctggcttcc aatccacata cggttataat catcgcggta aatacagtta acgcgtgttg
     1081 ttatattgta aacatattta taggtaccgg taaatgtttt tgtttttaaa tcgaaaaaaa
     1141 gtacgcctgc gtttttagta ccgcaccata atattccgtt ttcatcttca gctattgaga
     1201 taatttcatc ggcagaaaaa ttcaaagtat ccttcccatc aaatatgtag cttttaatta
     1261 gtttggtatt ataatcaaac tcatgcaagg cattatccca ggcaccgcaa taaaggcggc
     1321 cattgcttgc attaacaatg cagtttattg taatggcagc ttgggttata tccttgtgct
     1381 ttttaaatgc attggggctg cttacttttt ttaatgagtt aacaatagtg gctacataag
     1441 ccatagtatc tgataaaaaa atatcgtagg tactattgcc cgatacgtta taaagtccgt
     1501 taagttttct tgttcgcaca tcaaacaact gcatgccacc attgttgtaa ccaatccaaa
     1561 taaggttgcg caacttatcg
//
LOCUS       DAOMAA010000566         1579 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_89429_length_1579_cov_1.933727, whole genome shotgun sequence.
ACCESSION   DAOMAA010000566 DAOMAA010000000
VERSION     DAOMAA010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89429_length_1579_cov_1.933727"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(371..856)
                     /locus_tag="PLO59_07705"
     CDS             complement(371..856)
                     /locus_tag="PLO59_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQV01028.1"
                     /translation="MKNKNPDIKLRPTVVSDLSTLFQFQLDKEGGHLAAFMPKNHDDK
                     AAYMIKLTKLLNDPTVNNQTILLDNIIVGSVAKFLMGDTAEITYWIDRKFWGQGIATN
                     ALINFLAIEPSRPIYGRVAFDNYGSQKVLEKSGFVKIGSDKGFANARQIEIEEFIYKL
                     T"
     gene            complement(1090..>1579)
                     /locus_tag="PLO59_07710"
     CDS             complement(1090..>1579)
                     /locus_tag="PLO59_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01029.1"
                     /translation="GQTVKITGYISPEASDVYAGNYHVNDMVLYDDFLKMEHTDGLNF
                     IHFNADRFFTVFQNPSGNIKAQLYAGIGTGAVTPWTDTRLFNTFYRNPSIHFAGWGFC
                     AQAAPRLTFLNVVYIECALSVGYMYLWDILIERHKQNAQQQIVYSQRNFSLGFTLPLS
                     KQ"
BASE COUNT          500 a          273 c          275 g          531 t
ORIGIN      
        1 gttttttaaa atattttcat ccatgtttaa aagtgcagtt attaatttcc ggcaatgcct
       61 aacaaaaatc tatttatgca ttgtgtttta aaccatttca ataaccaagc aagcattagc
      121 gaagtttaag taataaatag ttagcgaaac cactaatttt tatatttttt gcaagcataa
      181 agataaagaa attataaagg ccggcaggga gcaaaatttc cggtttgcag gttagcgcac
      241 taaacttttg gatgccagta gaaatctgct attaatcatt tcttttaata gtactcgtat
      301 cagcccttta tgaatatgat tatacaacaa atcatcaata aattaatagg gcttttttgt
      361 ttacttcacg ttaagtaagc ttataaataa actcttcaat ctcaatttgt cttgcatttg
      421 caaaaccttt gtcggaacct atttttacaa aacccgattt ttctaatact ttttgcgaac
      481 cataattgtc aaaagcaaca cgaccatata tgggccttga tggctcaatg gcaaggaagt
      541 taattaatgc attagttgca attccctgtc cccaaaattt tctgtcaatc caataagtaa
      601 tttcagcggt gtctcccatt aaaaatttcg caacacttcc tacaataata ttgtcaagta
      661 aaatggtttg attatttact gttgggtcgt ttagcaattt tgtcaattta atcatgtaag
      721 cagctttgtc gtcatggttt tttggcatga atgcagccag atgcccgcct tctttatcaa
      781 gttgaaactg aaacaaagta ctcaagtcgg aaacaacggt tggcctaagt tttatatccg
      841 ggtttttatt tttcattgtt gctgtgttta ttaaaattta ttgtgcgttt aatttgtttt
      901 aaaaatcggt caaagattgg cttgctttgc gaagtctgac acaaggcttt gggtggcaaa
      961 ttaaaacgga ttttcatgaa ttgggttgtg caaaaaattg tttttagttt tttaaaacat
     1021 gttttggcac acaataacga acttcaacaa aacattacat aaaacagcag tattgcttta
     1081 ggttgttgat tactgtttac tcaaaggcaa agtaaagcct aatgaaaaat tgcgctgaga
     1141 ataaacaatt tgttgctgtg cattttgttt atgtctttca attaaaatat cccataggta
     1201 catgtagcct acacttaggg cacactctat atacacaacg ttcagaaaag ttagccgtgg
     1261 tgcagcctgc gcgcaaaagc cccagcctgc aaaatgtatt gaaggatttc ggtaaaaggt
     1321 gttgaacaaa cgcgtatctg tccagggagt tacggcaccg gtacctatgc ctgcgtataa
     1381 ttgggcttta atattacctg acgggttttg aaaaacagta aaaaaacgat cggcattaaa
     1441 atgaataaaa tttaaaccat cggtatgttc catttttaaa aaatcatcat acaaaaccat
     1501 atcatttaca tgatagtttc cggcataaac gtccgatgct tcggggctga tgtagcctgt
     1561 tattttaacg gtttgccct
//
LOCUS       DAOMAA010000567         1578 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_89513_length_1578_cov_2.556796, whole genome shotgun sequence.
ACCESSION   DAOMAA010000567 DAOMAA010000000
VERSION     DAOMAA010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89513_length_1578_cov_2.556796"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1049
                     /gene="rimO"
                     /locus_tag="PLO59_07715"
     CDS             <1..1049
                     /gene="rimO"
                     /locus_tag="PLO59_07715"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HQV01030.1"
                     /translation="SERYKPDLEKEITNVDAFFGTRDLQHLLKTLGANYKHELVGERM
                     VTTPKHFAYLKISEGCDRPCSFCAIPLMRGGHVSKPIEQIISEAKTLAAKGTKELLLI
                     AQDSTYYGLDLYKERKLAQLLDNLAQVEGIEWIRLHYAFPMGFPLDVLDVMAKHKNIC
                     NYLDIPLQHVNNDILKSMRRGTTREKTLQLLQTIRKKVPDITLRTTLISGYPGETDAQ
                     HQDLLNFINEVKFDRLGVFAYSHEEDTHAYNLIDNVADEVKQHRVAEIMNAQQEISWQ
                     LNQSKIGKTFNVLVDRKEGNYYIGRTEADSPEVDNEVIIDTQNNYLRVGSFINVHINK
                     AEEFDLFGVPTELK"
     gene            1071..>1578
                     /gene="bshC"
                     /locus_tag="PLO59_07720"
     CDS             1071..>1578
                     /gene="bshC"
                     /locus_tag="PLO59_07720"
                     /inference="COORDINATES: protein motif:HMM:NF021586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis BshC"
                     /protein_id="HQV01031.1"
                     /translation="MDFKVSHIPFAQTNAVNALVTDYYTGNKNLETYYAFEPNLNGIN
                     EAIEKRTNTAPNRKLLHHALQKQYSNLPTHNEVKSNLSSLLNSQTFTVTTGHQLNLFT
                     GPLYFIYKIASAIKLAQILNQQYSDKHFVPVYWMASEDHDFDEINHTYVFDKKINWTE
                     TNKGPTGSL"
BASE COUNT          585 a          293 c          275 g          425 t
ORIGIN      
        1 taagcgagcg ctataaaccc gacttagaaa aagaaattac caacgttgat gctttttttg
       61 gaaccagaga cctgcaacat ttacttaaaa cattaggtgc caattataag cacgaactgg
      121 taggtgaacg tatggttact acgcccaagc actttgctta tttaaaaatt tcggaaggct
      181 gcgataggcc atgctccttt tgcgctatac cattaatgcg tggtggacat gtttcgaaac
      241 cgattgaaca aataatatcc gaagcaaaaa cacttgcggc aaaaggaact aaagagcttt
      301 tattaattgc acaagacagc acttattatg gcttagactt atataaagaa agaaagcttg
      361 cccaattatt agataatttg gcacaagttg aaggtattga gtggattaga ttgcattatg
      421 cttttccaat gggattccca ttagacgttt tagatgttat ggccaaacac aaaaacattt
      481 gcaattattt agatatacca ctacaacatg taaacaacga catattaaag tcgatgcgca
      541 gaggtaccac acgcgaaaaa acactacaac tgttacaaac catacgtaaa aaagtaccgg
      601 atataacttt acgaactaca ttaatatcag ggtacccggg cgaaacagat gcacaacatc
      661 aggacttgtt gaactttatt aatgaagtga aatttgaccg tttaggtgtt tttgcctatt
      721 cgcatgaaga agatacacat gcatataatt taattgacaa tgttgccgat gaagtgaaac
      781 aacaccgtgt agccgaaata atgaatgcac aacaggaaat ctcatggcaa ttaaatcaat
      841 caaaaattgg aaaaactttt aatgtattgg ttgatagaaa agagggcaac tactacatag
      901 gccgaaccga agccgattcg cccgaagtag ataatgaagt aattattgat acccaaaata
      961 attatttgcg cgtaggcagc ttcattaatg tacacataaa caaagccgaa gagtttgatt
     1021 tatttggcgt accaactgaa ttaaaataat taaaaacaag cttattattt gtggatttta
     1081 aagtatcgca tattccattc gcacaaacca atgcagttaa tgcattggtt accgattact
     1141 atacaggcaa taaaaatctg gaaacatact atgctttcga accaaaccta aatggaatta
     1201 acgaagctat agaaaaacgc accaatacag cccccaatcg taaattgttg catcatgcat
     1261 tacaaaagca gtatagcaat ttgcctactc ataacgaagt aaaatcaaac ttaagttcat
     1321 tattaaacag ccaaacattt actgtaacta ccggccacca gctaaacctt tttacaggcc
     1381 ctttgtattt catttacaaa atagcatcgg caataaaact ggcgcaaatc cttaaccaac
     1441 aatatagcga caaacatttt gtaccggttt actggatggc ttcagaagat catgattttg
     1501 atgagataaa ccatacgtat gttttcgata aaaaaataaa ttggacagaa acaaataagg
     1561 gtccaaccgg aagtttac
//
LOCUS       DAOMAA010000568         1577 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_89605_length_1577_cov_3.283837, whole genome shotgun sequence.
ACCESSION   DAOMAA010000568 DAOMAA010000000
VERSION     DAOMAA010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89605_length_1577_cov_3.283837"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..295
                     /gene="nuoK"
                     /locus_tag="PLO59_07725"
     CDS             2..295
                     /gene="nuoK"
                     /locus_tag="PLO59_07725"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011584759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HQV01032.1"
                     /translation="MNSYLWLAAILFSIGVMGVLFRRNAIIILMCIELMLNAVNILFV
                     AFSVMHNNPAGQIMVFFIMAVAAAEVAVGLAILILMYRNMGSVDIDTMKKLKW"
     gene            314..>1577
                     /gene="nuoL"
                     /locus_tag="PLO59_07730"
     CDS             314..>1577
                     /gene="nuoL"
                     /locus_tag="PLO59_07730"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019945951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HQV01033.1"
                     /translation="MDSLIVLLIPLLPLIGFIISGTLNKQANHTLINGLTCGSVFVSF
                     LLSVILFYQLQSGSAPISYKLLNWIYVGALKLDLSFTVDALSSIMLLIITGIGFLIHV
                     YSTGYMHDEPDYARFFSYLNLFVFFMLLLVMGNNFVVMFVGWEGVGLCSYLLIGFWFK
                     NNAYNDAAKKAFVMNRIGDLGFLIGMFLILNKFGSLNYQTVFDLASHAATNDPTITVI
                     CICLFIGALGKSAQIPLYTWLPDAMAGPTPVSALIHAATMVTAGIYMIVRTNVLYTLS
                     PDAGFLVAVMGVATALFAATIGLKQNDIKKVLAYSTVSQLGYMFAALGVGAYETGMFH
                     VITHAFFKALLFLCAGSVIHAMSGEQDIRSMGGLRKKLPITHITFFIGTLAISGFPPF
                     SGFFSKDEILAKVHEYNGGFMLFTILAIT"
BASE COUNT          436 a          236 c          327 g          578 t
ORIGIN      
        1 tattaacagt tatttatggt tagcggccat actgtttagt ataggtgtaa tgggtgtatt
       61 gtttcgtaga aatgctataa ttattttaat gtgcattgaa ttaatgctca atgcagttaa
      121 tatattattt gttgccttta gtgtaatgca taataaccct gcaggtcaga taatggtatt
      181 ttttattatg gcagttgcag ctgcagaagt agcagttggt ttagcaattt taattttaat
      241 gtatcgcaat atgggctctg tagatattga tacgatgaaa aaacttaagt ggtaataaaa
      301 aagtaaaggc actatggatt ctttaattgt tttacttata cccttgttac cgcttattgg
      361 ttttattata agtggaacac taaataagca ggcaaaccat acattaatta acggattgac
      421 atgcggcagc gtttttgttt cctttctgtt gtcggtaatt ctgttttatc aactgcaaag
      481 tggttctgct cctatcagtt acaagctttt aaattggatt tatgtcggtg cgcttaagtt
      541 agacctgagt tttacagtag atgctttaag tagtatcatg cttttaatta ttacgggtat
      601 aggatttcta atacacgtat atagtacagg ttatatgcat gatgaacctg actatgcccg
      661 tttcttttct tatctgaacc tgtttgtatt ctttatgtta ttgctggtaa tgggcaataa
      721 ctttgttgta atgtttgttg gatgggaggg cgtaggttta tgttcttatt tattaattgg
      781 gttttggttt aaaaacaatg cttataatga tgctgcaaaa aaagcttttg taatgaatcg
      841 aattggcgat ttgggtttcc taatcggaat gtttttaata cttaataaat ttgggtcact
      901 taattaccaa accgttttcg acttagcaag tcatgcagct accaatgacc ctacaataac
      961 ggtaatttgt atttgtttat ttattggcgc tttgggtaaa agtgcacaga tacctctata
     1021 cacttggcta cccgatgcaa tggcaggccc tacacctgtg tcggctttaa ttcatgccgc
     1081 aaccatggta actgcaggta tttatatgat tgtaagaacc aacgtgttat atacattgag
     1141 tccggatgca ggttttttgg tggctgttat gggcgttgca acggcattgt ttgcggcaac
     1201 tattggtctt aaacaaaatg atataaagaa agtactagct tattcaaccg tttctcaatt
     1261 aggttatatg tttgctgcct taggtgtagg tgcatacgaa accggtatgt ttcatgtgat
     1321 aacacatgcc ttttttaaag cgctgctatt tttatgtgcc ggaagtgtaa tacatgccat
     1381 gagtggcgaa caagacataa gaagtatggg tggcctaaga aaaaaattac caattaccca
     1441 tattaccttt tttataggaa cgctagcgat tagtgggttt cctccgtttt ctggcttctt
     1501 tagtaaagac gagattttag ctaaggtaca tgagtataat ggaggtttta tgttgtttac
     1561 catcttggca atcactt
//
LOCUS       DAOMAA010000569         1571 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_90300_length_1571_cov_2.599604, whole genome shotgun sequence.
ACCESSION   DAOMAA010000569 DAOMAA010000000
VERSION     DAOMAA010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90300_length_1571_cov_2.599604"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="PLO59_07735"
     CDS             <1..191
                     /locus_tag="PLO59_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HQV01034.1"
                     /translation="ANRVSAALVKDFCNETTAPDLLLKHAAMRKATAVWRDKGSGRPT
                     KKDRRELDDFIWEGNFEI"
     gene            200..>1571
                     /locus_tag="PLO59_07740"
     CDS             200..>1571
                     /locus_tag="PLO59_07740"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01035.1"
                     /translation="MMLPYPNQKAEVYPTTNLLTDEFEQIKKQAQTFCLSTMGAACMQ
                     NAQPTGSFETMLTWLSQTNDFKKIIDNAESFVAEGYEDISRELNILRIENAVLSAEHC
                     LKILSLTQAIKSILLFYSNKHVLYPHAAQTANNIYYEPQISLLIENVFDEFGIVKSSA
                     SDALANIRKTLQRKRAEADRIYLQLINKYKKNGWISEIEESTRNGRRVISIVAEQKRS
                     LNGIVHDVSATGKTAYLEPEAAVEINNIIAALEQDERQEILRILRALTTNLRQFTLVL
                     NQYFELITITDFLRAKALFALQLKACMPLLSSQPFIQLKDARHPLLILQHIKSGKKTI
                     PFTLLLNQQNRILVISGPNAGGKTVCMKATALLQIMLQAGFLISTHESSVCGFFNNFL
                     IEIGDSQSIAYELSTYASRLQKMKMFLQEAGPKSLFIIDEFGTGTDPEMGGALAESIL
                     ESLNQKK"
BASE COUNT          529 a          306 c          315 g          421 t
ORIGIN      
        1 ctgcaaaccg tgttagtgca gccttggtaa aagatttttg taatgaaacc actgcacccg
       61 atttactgct taaacatgca gccatgcgca aagcaacggc tgtatggcgc gataaaggca
      121 gcgggcgacc tactaaaaaa gacaggcgcg agttagatga ctttatttgg gaaggcaatt
      181 ttgagatata gattttagta tgatgttgcc ctacccgaac caaaaagccg aagtgtatcc
      241 cactactaac ttgcttaccg atgagtttga gcaaattaaa aaacaggccc aaactttttg
      301 tcttagtact atgggtgcag catgtatgca aaacgcacaa cctacaggca gtttcgaaac
      361 gatgcttacc tggttaagcc aaaccaatga ctttaaaaaa attattgaca atgctgagtc
      421 gtttgttgcc gaaggctacg aagacattag ccgtgagctg aatattttgc ggattgaaaa
      481 tgcggttttg agtgctgagc attgccttaa aattttatcg ctaacacaag ccataaaaag
      541 cattttgctt ttttacagca acaagcatgt tctttatccg catgcagcac aaacagcaaa
      601 caatatttat tatgagccgc aaattagttt actgattgaa aatgttttcg atgaatttgg
      661 tattgtaaaa tcatcggcat cggatgcatt ggcaaacatc agaaaaacgt tgcaacgcaa
      721 acgtgccgaa gccgaccgca tctacttaca actgataaat aagtataaaa aaaacggatg
      781 gattagcgaa atagaagaaa gtacacgtaa tggccgtagg gtaatatcta ttgtagccga
      841 acaaaaacgg agtttaaatg gcattgtaca cgatgtatcg gccacaggca aaacggctta
      901 cctggagccc gaagcagcag ttgaaataaa caatataata gctgcattag aacaagacga
      961 gcgtcaagaa attttacgca tactacgggc gttaactacc aacctcaggc aatttacatt
     1021 ggtactaaac caatactttg aattaataac cattaccgat tttttacgag ccaaagcttt
     1081 atttgcactt caattaaaag catgcatgcc gctgctatca tcgcaaccgt ttatacaact
     1141 aaaagatgca cggcatccat tgttgatact gcaacacatt aaatcgggca aaaaaaccat
     1201 tccgtttact ttgttgttaa atcaacaaaa ccgcatattg gtaattagcg gacctaatgc
     1261 cggtggcaaa acagtatgca tgaaagctac tgcactttta caaattatgc tgcaagccgg
     1321 ttttttaatt agtacacatg aaagcagtgt atgtgggttt tttaataatt ttttaattga
     1381 gataggcgac tcacaaagca ttgcttatga attaagcacg tatgcatcgc gcttacaaaa
     1441 aatgaaaatg tttttacagg aagccggccc aaaatcgttg tttataattg atgagtttgg
     1501 taccggcacc gacccagaaa tgggtggagc cttagccgaa tctattttgg aatcgctaaa
     1561 tcagaaaaaa t
//
LOCUS       DAOMAA010000570         1571 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_90313_length_1571_cov_2.391821, whole genome shotgun sequence.
ACCESSION   DAOMAA010000570 DAOMAA010000000
VERSION     DAOMAA010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90313_length_1571_cov_2.391821"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..426)
                     /locus_tag="PLO59_07745"
     CDS             complement(139..426)
                     /locus_tag="PLO59_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01036.1"
                     /translation="MKKQTIKSVLFICLLFTTVLSHAQNSGKKPAPKPKIKPATKCTV
                     YFQGQPKSQLKVTEATAWCDSMPLLARGDNGIIYKLKSFDVMVIQKFFCGH"
     gene            complement(446..1405)
                     /locus_tag="PLO59_07750"
     CDS             complement(446..1405)
                     /locus_tag="PLO59_07750"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQV01037.1"
                     /translation="MDISVVIPLYNEDESLPELSAWITRVMVANNFTYEVIFIDDGSK
                     DKSWQVIENITQNNACFKGIKFRRNYGKSAALQKGFEAALGQVVITMDADMQDSPDEI
                     PDLYKMINNDGWDIVSGWKAKRYDPLMKTIPSKFFNWATRKMSGINNLHDFNCGLKSY
                     RSDVVKNIEVYGEMHRYIPVIAKWHGFPRITEKIVQHRARKYGVTKFGLERFINGFLD
                     LLSISFIGKFGKRPMHLFGTIGSIVFFVGLITTLYLGCAKLWDVYNDVPARLLTQRPS
                     FYIALVCMVLGTQLFLAGFLGELIARNSSTRNTYQIEKKIGLS"
BASE COUNT          465 a          297 c          283 g          526 t
ORIGIN      
        1 taaaagaaaa ttataatgct caggttctcg ttcatataag cgaagtctct tttcatgata
       61 cgtcgcacaa agctgagggg aacaaaaagc atctacctcc catgcttatt aattgtgttg
      121 aacaaatgat tcaatatttt aatggccaca gaagaatttt tgaattacca ttacatcaaa
      181 gcttttaagt ttatatataa tgccattatc gccacgtgcc aataatggca tgctatcgca
      241 ccaggctgta gcctcggtaa cctttaactg tgattttggc tgaccttgga aataaactgt
      301 gcatttggtt gccggtttaa tttttggttt tggtgccggt ttttttccac tgttttgcgc
      361 atgcgatagc acagttgtaa acaataaaca aatgaatagt actgatttta tggtttgctt
      421 tttcatgatg gtatttggtt ttaaattatg atagtcctat ttttttttcg atttgatatg
      481 tattacgagt tgaggagttg cgtgcaataa gctcgcctaa aaagcctgct aaaaataatt
      541 gtgttcccaa taccatacaa accaaggcaa tgtaaaatga gggtcgttgg gttaataatc
      601 gtgcaggcac atcgttatac acatcccaca attttgcaca tcctaaatat aaagtggtaa
      661 tcaagccaac aaaaaataca atggagccaa ttgtaccaaa aaggtgcatg ggtcttttgc
      721 caaatttgcc aataaagcta atggatagga ggtctaaaaa tccgtttata aaacgctcta
      781 aaccaaattt ggtaacgcca tatttgcggg cacggtgttg cactattttt tctgttatgc
      841 gcggaaagcc atgccactta gcaataacgg gtatgtagcg gtgcatttcg ccatacactt
      901 ctatattttt tactacatcg ctccgatacg atttaaggcc gcaattaaaa tcatgcagat
      961 tgtttatgcc cgacattttt cgtgttgccc agttaaaaaa cttgctgggt atggttttca
     1021 ttaagggatc gtatcgtttg gctttccaac ccgaaacaat atcccaacca tcattattaa
     1081 tcattttata caaatcgggt atttcatcag gcgagtcttg catatcggca tccatggtta
     1141 taaccacttg gcctaaagcc gcttcaaagc ctttttgcaa agcagcactt tttccataat
     1201 tgcgcctaaa cttaatgcct ttaaagcagg cgttgttttg tgtgatattt tcaataacct
     1261 gccacgattt gtctttgctg ccatcatcaa taaatattac ttcataggta aagttatttg
     1321 ctaccattac acgtgttatc caagccgata actcgggtag cgattcgtct tcattatata
     1381 agggtattac tactgaaatg tcaatcatct tgtaattatt tattgtacat cgtttgatgt
     1441 agcagaggaa gtactgttag gaatatttct tttatatatg gcagccatta ttaacgaaac
     1501 aattgcacct ccaattattt tattaaagta atcgcccatt aaagcattgc ctaaactggt
     1561 ttgatttata c
//
LOCUS       DAOMAA010000571         1568 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_90628_length_1568_cov_2.747521, whole genome shotgun sequence.
ACCESSION   DAOMAA010000571 DAOMAA010000000
VERSION     DAOMAA010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90628_length_1568_cov_2.747521"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..681)
                     /locus_tag="PLO59_07755"
     CDS             complement(130..681)
                     /locus_tag="PLO59_07755"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HQV01038.1"
                     /translation="MIVFSTFGIVILLLIIFLIFIFNELTKERILVNEAWSGIGAYLQ
                     QRLDAIPNSVEIVKGYAGHENKTLTDVIRARNESIAALTPEAQIEAAKNLGAAMLNFK
                     ALAENYPELKANEHFIKMQEQLVGLEEKINQSRRYYNGTVREYNQSIAVFPKNIVASL
                     FGFKVATFFAEDAEAGKAPKISF"
     gene            complement(846..>1568)
                     /locus_tag="PLO59_07760"
     CDS             complement(846..>1568)
                     /locus_tag="PLO59_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01039.1"
                     /translation="MANMQKHQLIIFNGKHEWCPATVLTDAVAAMLIWNNDDSVLKSN
                     YTKNQLKLIAKLKQKKQYLDASISYNALISVDNKPGFNNELSDLKNSAYYKNQLYASK
                     SVLINEQNNKQHYAVMMQQGIDTGYWINEVKMLQKLIQNEMLMASNQRLLSFLSLMAY
                     TLSNKALAQNNITAAQYFTNCYNIVDTSNAETNFLKAIVAARLGNNNQAIQQLNVAKQ
                     KGFTNWNRINAEPAFTNLNWRE"
BASE COUNT          450 a          271 c          270 g          577 t
ORIGIN      
        1 tcaatcagtt tgtaatattc gtctgattta aacagcttat cggcatattt tgtaaagcta
       61 attgcctgca cggcaatatg tgcaagcaac agcaatgcaa gtgttagctt tgctttcaat
      121 cgaaaaaaat taaaacgaaa tttttggtgc tttgccagct tctgcatctt cggcaaaaaa
      181 ggttgctact ttgaaaccaa aaagcgatgc cactatgttt ttaggaaaaa cggcaatgct
      241 ttgattatac tcacgtaccg taccattgta atatctgcgg ctttgattta tcttttcttc
      301 taagcctact aactgctcct gcatttttat aaaatgttca tttgctttta attcgggata
      361 gttttcggcc aatgctttaa agttaagcat ggcagcacct aaattttttg ccgcttcaat
      421 ttgtgcttca ggtgttaatg ctgcaattga ttcattgcgt gcgcgaatta catcggtaag
      481 tgttttattt tcgtgacctg catagccttt aacaatttca accgaattgg gtatggcatc
      541 taaacgctgc tgtaagtatg cacctatacc actccaggct tcgttaacaa gtatgcgctc
      601 tttggttagt tcgttaaaaa taaaaatcag gaatatgatt agtaataaaa ttacaattcc
      661 gaaagtgctg aatactatca taaataagtg attttaattt tgttcgaata taggattggt
      721 ttaacaaaca agcaacctaa tacatatttg aattgtcggt tttgtcttta tcgtttaaca
      781 gttattgacg ggataaaatt actacatcag ttagtaacta ttgcttataa taaatgcttt
      841 aaagattatt cgcgccagtt taaatttgta aatgcgggtt cagcgttaat gcggttccag
      901 tttgtaaacc ctttttgttt tgccacattt aattgttgaa tagcctggtt gttgttcccc
      961 agccgtgctg ccacaattgc ttttaaaaaa tttgtttcgg catttgaagt atctactatg
     1021 ttataacaat ttgtaaagta ctgtgcagcg gttatattat tttgtgccag tgccttatta
     1081 ctcagtgtat atgccattaa acttaaaaaa cttaacaacc gttgattact ggccattagc
     1141 atttcatttt gaatgagttt ttgtaacatc ttaacttcgt taatccaata gccggtgtca
     1201 ataccttgtt gcatcatcac ggcataatgt tgtttgttat tttgctcatt aataagcacg
     1261 cttttactgg cgtaaagctg atttttatag tatgctgaat tttttaaatc cgataattca
     1321 ttattaaatc cgggtttatt atcaacacta atcaaagcat tatagcttat tgaagcatcc
     1381 agatattgct tcttttgttt gagcttagca attagtttga gttggttttt tgtatagttc
     1441 gattttaata cggaatcgtc attattccaa atcaacattg cagcaacggc atctgttaat
     1501 acagtagcgg gacaccattc atgcttgcca ttaaatataa ttagctgatg cttttgcata
     1561 ttagccat
//
LOCUS       DAOMAA010000572         1563 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_91180_length_1563_cov_2.326923, whole genome shotgun sequence.
ACCESSION   DAOMAA010000572 DAOMAA010000000
VERSION     DAOMAA010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91180_length_1563_cov_2.326923"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1476
                     /locus_tag="PLO59_07765"
     CDS             <1..1476
                     /locus_tag="PLO59_07765"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HQV01040.1"
                     /translation="SWYKEGVVIVDAVRPDNLIEVGHYDTSPQTGGGFDGAWGVYPYL
                     PSGNVLVSDMSEGLFVLTPTYQRACYLEGLVSDSITLQPINGVAVNILTRVHIENTNF
                     TGMYKTGTATAGLYDIVFTKQGYISKIVTGVSLSNGVVTNLNVLLQPLTTVGITGSVT
                     DVVAGINNATVFMTGINGNYTLAADANGNFTANVYPGSYDITVGKWGYVTQCFGGINI
                     TPGSTLNYQLTKGYYDDFSLDFNWNTSATANTGVWVREVPVGTTLNNNIANPNADVIT
                     DCNAQAFVTGNAGGGSGNDDIDGGAVVLTSPVFDLTTYASPVLSYARWFFNAQSGGNA
                     PNDSLTIYISNGLQTVVVEAVGNSLVNNSKWLNKTYNLNSLITPTANMTLIVKAEDIS
                     PGNVTEAGFDVFQITDGIAAIDEQILPVSAMPNPFNDATKIIMPVAGLLTVTNVTGQV
                     VYKDVLEADKPVYIGKNWQAGVYFLKFVSGNNVFSGKLIKQ"
BASE COUNT          467 a          270 c          347 g          479 t
ORIGIN      
        1 tcctggtata aagagggtgt tgtaattgtt gatgcagtgc gccccgataa tttaattgaa
       61 gtggggcatt acgatacatc gccacaaaca ggtggtggct ttgatggtgc ctggggtgtg
      121 tatccatatt tgccttcggg taatgtattg gttagcgata tgagcgaagg tttatttgta
      181 ttaacaccaa cctatcaacg tgcttgctat ttggagggcc ttgtaagcga tagtataaca
      241 ttacaaccca taaatggcgt tgctgtaaat attttaacaa gggtacatat tgaaaacacc
      301 aattttaccg gtatgtataa aaccggcacg gcaacagcag gtttatacga tattgttttt
      361 acgaagcaag gttacattag caaaatagta accggtgtaa gtttaagcaa tggtgtggtt
      421 accaatttaa atgtgttgtt gcaaccttta actacagtgg gcattacagg cagtgtaacc
      481 gatgtggttg ccggtattaa taatgcaacg gtttttatga cgggcattaa tggaaattat
      541 acacttgcag ccgatgctaa tggtaatttt acagccaacg tatatccggg tagttatgac
      601 attacggtgg gcaaatgggg ttatgttacc caatgttttg gtggcataaa tattaccccg
      661 ggcagtactt taaattacca actaactaaa ggttattatg atgatttttc gctcgatttt
      721 aattggaaca catcagcaac tgccaatacc ggagtctggg tacgagaagt gcctgttggt
      781 acaacactaa ataataatat tgctaacccc aatgccgatg ttattaccga ttgcaatgca
      841 caggcatttg ttaccggcaa tgcaggcggt gggtccggta acgatgatat tgacggaggt
      901 gcagttgtat taacatcgcc tgtttttgat ttaacaactt atgcaagccc ggttttaagt
      961 tatgcccgtt ggttttttaa cgcacaatct ggcggcaatg cgccaaatga cagtttaacc
     1021 atatatatca gcaatggttt acaaacagta gttgttgaag cggtaggcaa ttcattggta
     1081 aataacagca agtggttgaa caaaacctat aatttaaata gtttgattac gcccacggca
     1141 aatatgactt tgattgtaaa agccgaagac atttcgcccg gcaatgttac cgaagcaggt
     1201 tttgatgtgt ttcaaataac cgatggtatt gccgcaattg acgaacaaat tttaccagta
     1261 agtgccatgc ccaatccgtt taacgatgct actaaaatta taatgccggt tgcaggcctg
     1321 ctaactgtta caaatgtaac aggccaggtt gtttataaag atgtattgga ggctgataaa
     1381 ccggtatata ttggtaaaaa ctggcaagcc ggagtttact ttttaaaatt tgtttcgggc
     1441 aataatgtat tttcaggtaa actaattaaa caatagcaaa taccagcgcc tgctatcgtt
     1501 tagtataagc aacatttacg cattgatgct tataaactgt atatgtaact ggtttgattt
     1561 tta
//
LOCUS       DAOMAA010000573         1562 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_91301_length_1562_cov_2.266755, whole genome shotgun sequence.
ACCESSION   DAOMAA010000573 DAOMAA010000000
VERSION     DAOMAA010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91301_length_1562_cov_2.266755"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PLO59_07770"
     CDS             <1..308
                     /locus_tag="PLO59_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01041.1"
                     /translation="KDLLLPLFTDKFDLLIDYNWATLLYLHKHLQINTVLLKTIMYET
                     DYDFDYRNFEQIKFVEPIPYNQVFMYKRKFIPYTSALDLLFSLGPKSSQYLTNSALH"
     gene            complement(305..871)
                     /locus_tag="PLO59_07775"
     CDS             complement(305..871)
                     /locus_tag="PLO59_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017257082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01042.1"
                     /translation="MQDLEPFYRWRDLYVANEDALSPFYGQEYSEFEYSNKIYNYYIH
                     PQWDDFGSNTLYLKIIYADYETGFAVIEFIGEWNDAINNDIMLLKRDIIDVLTLEGIN
                     KFILIGENVLNYHASDDCYYQEWFDDIEDGWIVAINFREHVLNEFIKNNIDYYLVFGG
                     ELNQVNWRKLSPNQLFELANALVNKRLN"
     gene            complement(873..>1562)
                     /locus_tag="PLO59_07780"
     CDS             complement(873..>1562)
                     /locus_tag="PLO59_07780"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011586415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA];
                     GO_process: GO:0097173 - N-acetylmuramic acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HQV01043.1"
                     /translation="IPNIEALVVNIIEKMKQGGRLFYIGAGTSGRLGIVDASECPPTY
                     GIPQGMVIGIIAGGDSAIRKAVEFAEDDTEQGFTDLKLYNVNQKDIVVGIAASGSTPY
                     VVGALAKCNANGITTACIVCNTRTAVAAQATYKIECVVGPEFVTGSTRMKAGTAQKLI
                     LNMISTSVMIGLGKVKGNKMFDMQLSNNKLVSRAVNMLMEEINVDQATAQNLLNLHGN
                     VRNAAAAFNKK"
BASE COUNT          460 a          328 c          242 g          532 t
ORIGIN      
        1 ataaagattt attgctgccc ctgtttactg ataagtttga tttgttgatt gactataatt
       61 gggctacttt attgtattta cacaagcatt tacaaataaa taccgtattg ctgaaaacca
      121 ttatgtatga aaccgattat gattttgatt atcggaattt tgagcaaata aaatttgttg
      181 agcccattcc atacaatcag gtttttatgt acaagcgcaa atttattcca tacacaagtg
      241 cattggattt attgttcagt ttggggccta agtcttcgca atatttaacc aactcagcgt
      301 tgcactaatt aaggcgttta tttactaagg cattagccag ctcaaacaat tgattaggcg
      361 aaagtttgcg ccaatttacc tggttaagtt caccgccaaa caccaaatag taatcaatat
      421 tatttttgat aaattcattc aatacatgtt ctctgaaatt gatagctaca atccatccat
      481 cttcaatatc atcgaaccac tcctgatagt aacagtcatc acttgcatga taatttaaaa
      541 cattttcgcc tattaatata aacttgttta tcccctcaag ggttaataca tctattatat
      601 cacgttttaa cagcatgatg tcgttgttta tggcatcatt ccactcgcca ataaattcaa
      661 taacagcaaa acctgtttcg taatcggcat aaataatttt aaggtacagt gtgttgcttc
      721 caaaatcatc ccactgcggg tgtatgtagt aattgtatat tttattactg tattcaaact
      781 cgctgtattc ctggccataa aaaggcgaaa gcgcatcttc attagccaca tataaatcgc
      841 gccaacgata aaaaggttct aaatcttgca tcttattttt tattaaaagc tgctgcagca
      901 tttcttacat tgccatgtaa gtttaataaa ttttgtgctg tagcctgatc aacattaatt
      961 tcttccatca acatattaac ggcacggctt actaatttgt tgtttgacaa ctgcatatca
     1021 aacattttgt ttccttttac tttccccaat cctatcataa ccgatgtgct tatcatattt
     1081 aaaattaatt tttgtgcagt gccggctttc atacgcgtac taccggtaac aaactcgggt
     1141 ccaaccacac attcaatttt ataagtagct tgtgctgcta ctgccgtacg tgtattgcaa
     1201 actatgcatg ctgtagtaat cccatttgca ttgcatttgg ctaaagcgcc caccacataa
     1261 ggtgtactgc ccgatgcagc tatgcctact acaatatctt tttggtttac gttgtacaat
     1321 ttcaaatcag taaagccttg ttctgtatcg tcttcggcaa actcaacagc ttttcgtatg
     1381 gcactatcgc caccggctat tatgcctata accatgcctt gcggtatgcc gtaggttggc
     1441 ggacattcgc ttgcatcaac aatacctaag cggccactgg tacctgcacc aatgtaaaat
     1501 aaacgaccac cttgcttcat tttttcaatg atgtttacaa ccaatgcttc aatattaggt
     1561 at
//
LOCUS       DAOMAA010000574         1562 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_91303_length_1562_cov_2.213670, whole genome shotgun sequence.
ACCESSION   DAOMAA010000574 DAOMAA010000000
VERSION     DAOMAA010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91303_length_1562_cov_2.213670"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1483
                     /locus_tag="PLO59_07785"
     CDS             <1..1483
                     /locus_tag="PLO59_07785"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HQV01044.1"
                     /translation="ATTINNLYLSYADSTYNQTIIVPSFVYDLKQLQTCALLKTQNKF
                     DTLFVSAQVEKEGKVYHYNISHPAGGKTYLPILKQEKFPKLGFKSISGKLTYNYLNDI
                     ELGIDAQIADLHINHWRLSESDVVMPQTQFIGTIKIGVQNLSIDSTSVLKLNNAQAML
                     YANFSKAEKNSYAFKITMEPTQADTFFKALPLGMFNTLRGIKTKGTLSFNLNFELNGS
                     KPDDVKFEASLQKKNFSIINFGAEYYGRINQTFLFDALDGDRLVRQLTVGPESNTFTP
                     FADISSYLKYAVLTSEDGSFMYHHGFNEDAFRQSIATNYKQGRFARGGSTITMQLVKN
                     CFLSRDKTVSRKAEEALIVWLIENCGLVSKERMLEVYLNVIEWGPDVYGIGEAAGFYF
                     AKQPKDLTLAESIFLASIIPHPKYFKYSFSQDATLKPFLTSYYKLLSNKMLGRNWITD
                     ADTVGLTANVILTGPAKNFVLQATDSLPMPADSILQQDLQIVD"
BASE COUNT          543 a          278 c          279 g          462 t
ORIGIN      
        1 agctacaacc attaacaacc tgtatttatc ttatgcagat agcacctata accaaaccat
       61 tatagtacct tcatttgttt atgatttaaa acaattgcaa acctgtgcat tattaaaaac
      121 acaaaataag ttcgacactt tatttgtttc ggcccaagtg gaaaaagagg gtaaagtgta
      181 tcattataat atatcacatc cggccggtgg taaaacatac ttaccaattt taaagcaaga
      241 aaaatttccg aaactgggtt ttaaatccat tagcggtaaa cttacctata actaccttaa
      301 cgacattgaa ttaggtattg atgcacaaat tgccgacctg catataaatc attggcgctt
      361 atctgaaagt gatgtggtaa tgcctcaaac gcaatttata ggtaccatta aaattggtgt
      421 tcaaaattta tctattgaca gtacatctgt tttgaagtta aataatgcac aggcaatgtt
      481 gtatgcaaat ttttcgaaag ctgaaaaaaa cagctatgca tttaaaatta caatggaacc
      541 cacccaagcc gacacttttt ttaaagcatt gcccttaggc atgtttaata cactgagagg
      601 cataaaaaca aagggtacct taagttttaa tttaaatttt gaattaaatg gttcgaaacc
      661 tgatgatgta aagtttgaag catcattaca aaagaagaat tttagcatta taaactttgg
      721 tgccgagtac tatggtcgaa taaatcaaac ttttttgttt gatgcattag atggcgacag
      781 gttagtaagg caattaaccg ttggacccga gagtaatact tttacacctt ttgcagatat
      841 aagcagttac cttaaatatg cagtactaac cagcgaagac ggttcattta tgtaccatca
      901 cggctttaat gaggacgcat tcaggcagtc aatagctact aactacaaac agggacgttt
      961 tgccagaggc ggcagcacca taaccatgca attggttaaa aactgctttt taagtcgcga
     1021 taaaacagta tcgcgtaaag ccgaagaagc attaatagta tggcttatcg aaaactgtgg
     1081 tttggtaagt aaggagcgca tgctcgaagt atatttaaac gtaattgaat ggggccctga
     1141 cgtatatggc ataggcgaag ctgcaggctt ttattttgcc aaacaaccta aagatttaac
     1201 tttagcagaa agcatttttt tagcaagtat aattccacac ccaaaatatt ttaaatattc
     1261 gtttagtcag gatgcaacat taaagccttt tttaacatca tattataaat tactttctaa
     1321 taagatgtta ggccgcaatt ggataaccga tgccgataca gttggcctaa cagcaaatgt
     1381 tatattaaca gggccagcta aaaactttgt attacaggca accgatagtt tgccaatgcc
     1441 agccgactcc atattgcaac aagacttaca aattgttgat tagagacaaa aattgcgcga
     1501 attaattttt gttattggcc gcaagcagat ataaaacggc catacgaata gcaacaccgt
     1561 tt
//
LOCUS       DAOMAA010000575         1559 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_91641_length_1559_cov_2.317154, whole genome shotgun sequence.
ACCESSION   DAOMAA010000575 DAOMAA010000000
VERSION     DAOMAA010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91641_length_1559_cov_2.317154"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="PLO59_07790"
     CDS             complement(<1..434)
                     /locus_tag="PLO59_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01045.1"
                     /translation="MKALKTLTALLFCSLIITTTYASELIVKSNHFPIAVSVDNGPLS
                     AADNQVSVKYIPEGYRQIAIYSMVSNAHRYAMPQLVFSSKVYIPRAVDLMAVVTQFGK
                     FKIVNTIAHAPQTPVNNMPTPVCNPIPVCGTTVPAYIMNNAD"
     gene            647..1243
                     /locus_tag="PLO59_07795"
     CDS             647..1243
                     /locus_tag="PLO59_07795"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016195340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HQV01046.1"
                     /translation="MAFELPALPYATTALEPHIDARTMEIHHGKHHQAYVTNLNNALA
                     GADTTSIEDICKNISKYPAAVRNNGGGHYNHSLFWEIMAPNAGGAPTGKLAEAINTAF
                     GSFDDFKTKFAQAGATRFGSGWAWLVKLDDGSLAVTSTANQDNPLMDVAEVKGLPLLG
                     CDVWEHAYYLNYQNRRPDYITAWWNVVNWDAVSKRFEK"
     gene            1375..>1559
                     /locus_tag="PLO59_07800"
     CDS             1375..>1559
                     /locus_tag="PLO59_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01047.1"
                     /translation="MKKQLLAITCMLVTFSTQAQLLGQGGGSYQISHAECISPAQQKL
                     IWQDVQQNIKTLELKGA"
BASE COUNT          502 a          301 c          303 g          453 t
ORIGIN      
        1 tagtcggcat tgttcattat atacgcaggt actgtagtgc cacatacagg tattgggtta
       61 caaacaggag ttggcatgtt gttaacaggt gtttgtggtg catgcgcaat ggtgtttaca
      121 attttaaatt taccaaactg agttactaca gccattaaat caactgcacg tggtatataa
      181 accttgctgc taaaaaccaa ttgtggcata gcataacgat gtgcatttga aaccatgctg
      241 tatattgcta tttgcctgta accttcgggt atgtacttta ccgacacctg attatcggca
      301 gcgcttaagg gaccattgtc aacagaaacg gcaattggaa aatggttcga ctttacaata
      361 agttccgaag cataagtagt tgtaataatc aaactacaaa acaataaagc ggtaagtgtt
      421 tttaaagctt tcataatgtg tcctcctttt aattttaagt gtatgcagtg gtaatttcaa
      481 taccaatgcc aaaacatttt tataaaacgg ttgttcatca atcgttcaca acaatttttc
      541 gactgcataa aattattttc aattgcaacc gacaataaat gcggctatgg ttgctcaaac
      601 cattgcatta cgtacctttg aaaactcatt ttaaaacata tcaatcatgg cattcgaatt
      661 acccgcatta ccttatgcaa caacagcttt agaacctcac attgatgcac gcaccatgga
      721 aatacaccat ggcaaacatc atcaggcata cgtaaccaat ttaaacaatg cattggccgg
      781 tgccgataca acttctattg aagacatttg taaaaacatt tcgaaatacc cggcagccgt
      841 gcgcaataat ggtggcgggc actacaacca cagtttgttt tgggagatta tggcacccaa
      901 tgcaggtggc gcacccaccg gtaaattagc cgaagccatc aatacggcct ttggtagctt
      961 tgatgatttt aaaaccaaat ttgcacaagc aggtgcaaca cgttttggca gcggatgggc
     1021 atggcttgtt aaacttgacg atggcagctt agctgtaaca agcacagcca accaggataa
     1081 tccactaatg gatgttgccg aagtaaaagg cttgccttta ttgggatgcg atgtatggga
     1141 acatgcatac tatctgaact atcaaaaccg caggcccgat tatattacgg cctggtggaa
     1201 tgtggttaac tgggatgctg tatcgaaacg atttgaaaaa taaaatgtat taaaaccggc
     1261 tatgctggtt tttttatgcg cattagctat cgcttatatt taaagttaaa tttcaaagcg
     1321 taatatgaat ggtttaatta ttaaattaga acattcaatt ttaaaaccat tgctatgaaa
     1381 aaacaactac ttgctattac atgtatgctt gttacattta gtacacaggc acagttactg
     1441 ggtcaaggtg gaggctcata tcaaatttca catgccgaat gtataagccc tgcacaacaa
     1501 aaattaattt ggcaggatgt acaacaaaac attaaaacgc ttgaactaaa aggcgcttt
//
LOCUS       DAOMAA010000576         1557 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_91799_length_1557_cov_3.853529, whole genome shotgun sequence.
ACCESSION   DAOMAA010000576 DAOMAA010000000
VERSION     DAOMAA010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91799_length_1557_cov_3.853529"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..>280
                     /locus_tag="PLO59_07805"
     CDS             101..>280
                     /locus_tag="PLO59_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HQV01048.1"
                     /translation="MSNIYCVIMAGGIGSRFWPMSRNAKPKQFLDILGNGKTLIQQTF
                     DRLTLLCPPENILVVT"
     assembly_gap    281..290
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <291..1199
                     /locus_tag="PLO59_07810"
     CDS             <291..1199
                     /locus_tag="PLO59_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019149507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HQV01049.1"
                     /translation="TNEDYAQLVAEQLPAILPQHILSEPARKNTAPCIAYAAYKIAAI
                     NPEALMVVAPSDHIITNTANFITAAQKGIDFASKHNCLLTLGIQPSRPDTGYGYIQVI
                     DDKHDEANKISKVKTFTEKPNLEMAKFFLLSGEFLWNTGITISSVQATMEAIKFHLPE
                     LSVLFEEGIGLYNTTNETEFIKRIYPVCTNISIDFGVMEKAKNVYVLSTEFGWSDLGT
                     YGSLYEHIKHDNNGNAVVGKNVMLYDSKNCIVNVPKNKLVVIQGLEDYIVVERDDVLL
                     ICKKDDEQNIRQYVNDVKINKGDKYV"
     gene            1206..>1557
                     /locus_tag="PLO59_07815"
     CDS             1206..>1557
                     /locus_tag="PLO59_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009228420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HQV01050.1"
                     /translation="MQSISATVIKSVGSSFTVIDPSGHQHQCKIKGTFRIKDIDSTNP
                     IAVGDKVTVTFDTGTEFGLITAIEPRQNYIIRKSTNLSKQTQILAANVDMAVLIVTPR
                     LPKTSTGFIDRFIAT"
BASE COUNT          533 a          266 c          300 g          448 t
ORIGIN      
        1 aataggtatg taatactgtt accggcaagt taactgcaat aacaattgta ataacgccct
       61 aatcaaataa aattacattt gcgcccttta taaaagtaat atgagtaaca tttattgtgt
      121 gataatggca ggaggcattg gcagccgttt ttggcctatg agccgcaatg ccaagcctaa
      181 acagttttta gatatattag gtaacggtaa aacattaata caacaaactt tcgaccggtt
      241 aacgttgctt tgcccacccg aaaatatttt agtagttact nnnnnnnnnn actaatgaag
      301 attatgcgca attagttgcc gagcaattgc ctgctatttt accacaacat attttaagtg
      361 agccggctcg taaaaacacg gcaccatgta ttgcttatgc ggcttataaa attgcagcca
      421 taaatccgga agcattaatg gtggttgcgc ccagcgatca cattattacc aatacagcaa
      481 actttattac tgcagcacaa aaagggattg attttgcatc gaaacacaat tgtttattaa
      541 ccttaggcat acagcccagc cggccagata cgggctatgg ttatatacag gttattgatg
      601 ataaacatga tgaggcaaat aaaattagta aagtaaaaac ctttactgaa aaaccgaacc
      661 tcgaaatggc aaaattcttt ttgcttagtg gcgagttttt atggaatacc ggtattacca
      721 taagcagcgt acaggcaact atggaagcca ttaaatttca cttgcccgaa ctgagtgtat
      781 tgtttgaaga aggtattggt ttatacaata caactaacga aaccgaattt attaagcgta
      841 tttacccggt ttgcaccaac atatcaattg actttggtgt tatggaaaaa gcaaaaaacg
      901 tttatgtgtt aagcactgaa ttcgggtgga gcgatttagg tacttatggt tcattatatg
      961 agcacattaa gcacgataac aatggcaatg ctgtagttgg taaaaatgta atgctatacg
     1021 atagtaaaaa ttgcatcgtt aatgtgccta aaaataaatt agttgtaata caaggtttgg
     1081 aagattacat tgttgttgag cgtgacgatg tattgcttat atgtaaaaag gacgatgaac
     1141 aaaacatcag acaatatgta aacgatgtaa aaattaataa aggcgataag tatgtgtaat
     1201 acatcatgca aagcataagc gctacagtaa ttaaatcggt tggcagctcc tttacggtta
     1261 ttgacccgag tgggcatcaa catcaatgta aaattaaagg cactttcaga attaaagata
     1321 ttgacagtac caaccccatt gccgttggcg ataaagtaac cgtaacgttt gatacaggta
     1381 ctgaatttgg gttgataaca gccattgaac caaggcaaaa ttacatcatc cgaaaatcaa
     1441 ccaatttaag taagcaaaca caaattttag ctgccaatgt tgatatggct gtgctaattg
     1501 ttacaccgcg tttacctaaa accagcactg gttttattga tagatttatt gctacgg
//
LOCUS       DAOMAA010000577         1557 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_91825_length_1557_cov_2.921438, whole genome shotgun sequence.
ACCESSION   DAOMAA010000577 DAOMAA010000000
VERSION     DAOMAA010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91825_length_1557_cov_2.921438"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /locus_tag="PLO59_07820"
     CDS             <1..845
                     /locus_tag="PLO59_07820"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HQV01051.1"
                     /translation="NLFGYMFLPDYIEQYGIANYKASIKAIENITQFVSCEYAIRPFI
                     IKYPQEAINQMLGWSKHKNQHVRRLASEGMRPSLPWAMALPAFKKNPEPILPILENLK
                     ADSSDYVRRSVANNLNDISKDNPQVVLALAKKWKGKNELTDCIIKHGCRTLLKQGNIE
                     ILKLYALQNSAKINVSQFNLQYEKIQTGTKLPFAFSIVNRHTTKQNIRVEYAIYFMLK
                     NGTHYKKIFKITEGILNAGEARVINKAHNFKLITTRQYYTGKHYLGIIINGTEKVKTQ
                     FVLI"
     gene            893..1237
                     /locus_tag="PLO59_07825"
     CDS             893..1237
                     /locus_tag="PLO59_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004920185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HQV01052.1"
                     /translation="MSDILKCPACMSEYTYVDNDMLVCSQCFYEWSMHTEATNNTETE
                     KILDANGNELQNGDSVIVVKDLPVKGAPKPVKAGTKVKNIRLNFESDHNIDCKIEGFG
                     SMALKSEFVRKA"
     gene            1312..>1557
                     /locus_tag="PLO59_07830"
     CDS             1312..>1557
                     /locus_tag="PLO59_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HQV01053.1"
                     /translation="MKHLACILLCISVFKYAIGQNSQTNTIPAATPLMGWMSWNFFGD
                     NITEQSIKEMADAMVASGMRDAGYTYIFIDDGWQGGRD"
BASE COUNT          560 a          275 c          280 g          442 t
ORIGIN      
        1 aaaacctttt tggctatatg tttttacccg attatattga gcagtacggc attgcaaatt
       61 ataaagcatc cattaaagcc attgaaaata ttacccaatt tgttagttgc gaatacgcta
      121 tcaggccttt tataattaaa tatccgcaag aagccataaa tcaaatgtta ggttggagta
      181 aacacaaaaa ccaacatgta agacgtttgg ccagcgaagg tatgcggcca agcttaccat
      241 gggctatggc attgccggca tttaaaaaaa atccggaacc gatattgccc attttagaaa
      301 accttaaagc cgattcatcg gattatgtga ggcgtagtgt tgccaacaac ttaaatgata
      361 tttcgaaaga taatccgcaa gttgtattgg cattggctaa aaaatggaaa ggcaaaaatg
      421 aactaaccga ttgcattata aaacacggct gtcgtacttt attaaagcaa ggcaacatcg
      481 aaattttaaa attatatgcc ttacaaaact ccgccaaaat caatgtaagt cagtttaatt
      541 tgcaatatga aaaaatacaa acaggtacta agttaccatt tgcatttagt attgtaaacc
      601 ggcatacaac caaacaaaat atcagagttg aatacgcaat ttactttatg ctaaaaaacg
      661 gaacgcacta taaaaaaata tttaaaataa ccgaaggcat tttaaacgct ggagaggcac
      721 gcgtaattaa taaagcacac aactttaagc ttataactac acgtcaatat tatacaggca
      781 aacattatct gggcattatt attaacggaa cagaaaaagt gaaaactcaa tttgtgctca
      841 tttaattgta accattaatt ttgcgcaaca attaattttt aattttaata acatgagcga
      901 tatacttaaa tgtccggcat gcatgtcgga atacacatac gttgataatg acatgttagt
      961 ttgttcgcaa tgcttttatg aatggagcat gcatacagaa gcaacaaaca atacagaaac
     1021 cgaaaaaata cttgatgcca atggcaatga attgcaaaat ggcgactcgg taattgtggt
     1081 aaaagatttg ccggttaaag gtgcgcccaa acctgtaaag gcaggcacta aggttaaaaa
     1141 cattcgcctt aactttgaat cggatcataa tattgattgc aaaattgaag gctttggatc
     1201 aatggcatta aaatctgaat ttgtaagaaa agcttaaata caatactgca aatgctttta
     1261 acgcaataca tactgcctgc tgctcatttt cacatctgat ttatttacaa catgaaacac
     1321 cttgcatgca tactactttg tatatcagtt tttaaatacg ctatagggca aaactcgcaa
     1381 accaatacca tacctgcagc tacacctcta atgggttgga tgtcgtggaa tttttttggt
     1441 gataatatta ccgaacaatc tattaaagaa atggctgatg ctatggtagc cagcggcatg
     1501 cgcgatgcag gctacacgta tatttttatt gatgatggct ggcaaggtgg gcgcgat
//
LOCUS       DAOMAA010000578         1556 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_91918_length_1556_cov_3.416389, whole genome shotgun sequence.
ACCESSION   DAOMAA010000578 DAOMAA010000000
VERSION     DAOMAA010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91918_length_1556_cov_3.416389"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="PLO59_07835"
     CDS             complement(<1..423)
                     /locus_tag="PLO59_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002675428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HQV01054.1"
                     /translation="MSLQEKINNDLKEAMKAKNEAALRALRAVKSAILLENTSGKGEL
                     DHAAETKLLQKLVKQRKDSIEIYNQQGRTDLAQTEIEEVHIIEKYLPAMLSDDEIKSQ
                     LMQLIAQVGATSAADMGKVMGAATKHFAGKADNKTVSAL"
     gene            complement(458..>1556)
                     /locus_tag="PLO59_07840"
     CDS             complement(458..>1556)
                     /locus_tag="PLO59_07840"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HQV01055.1"
                     /translation="KTNIDFHEKIQLNGYLAYGFNDARFKFSVGADYFFSRKPRELIG
                     VQFKNDIQQLGLSDNAFQTDNILSSLFRRNPANKLTDTEIKKLYYEKEWFAGFSNRLT
                     LSNSNLNPLGILSYAYYVDDAKTIVRENIIQSEISLYTRYMHQEKFVYTKHGRTSLGS
                     KYPAIQANVTVGFKGVFSSDFTYTKVDLKVSDIIRMAPIGYGYYSMDVGRVFGVVPYP
                     LLFVHPGNETYFYDFAAFNMMNYYEFVSDFYGSLYYTHHFDGFFLDKIPLLRKLKWRE
                     IASIKAISGGLNKGNRDILVNPNAFSNLSQPYLEAGIGVENILKIIRVDFLWRMNYID
                     DDYKLLYKERFGEESNLPTRFGIRAGLQLTF"
BASE COUNT          443 a          302 c          285 g          526 t
ORIGIN      
        1 caatgccgat actgttttgt tatcggcttt gcctgcaaaa tgtttggttg cagcgcccat
       61 tacttttccc atatcggcag ctgaggtagc accaacctga gcaataagct gcataagttg
      121 gctttttatt tcatcatcac taagcatggc cggcaaatat ttttcgataa tatgtacttc
      181 ttctatttca gtttgtgcaa ggtcggtacg accttgttga ttataaattt cgatactatc
      241 cttacgttgc tttaccagct tttgtaaaag tttggtttcg gccgcatggt ccagttcgcc
      301 tttgccgctt gtgttttcga gtaatattgc cgacttaaca gcacgcaggg cacgtaaggc
      361 cgcttcgttt ttggctttca tagcctcttt taaatcgtta tttatttttt cttgtaaact
      421 cattttttaa aatatttaaa tgcaaataaa caattaatca gaaggtgagt tgcagccctg
      481 cccttatgcc aaagcgggta ggcagattgc tttcttcgcc aaatcgctct ttgtaaagta
      541 acttgtaatc gtcatcaata taattcatgc gccataaaaa atcaacacga attattttta
      601 aaatattttc aacgcctata cctgcttcta aatagggctg gcttaagtta ctaaatgcat
      661 tggggttaac cagtatatct ctgttgcctt tatttaagcc gccactaatt gcttttatgc
      721 tggctatttc gcgccattta agtttacgta acaagggtat tttatctaaa aagaaaccat
      781 caaaatgatg tgtgtaatat agtgagccat aaaaatcgct cacaaattca taatagttca
      841 tcatgttaaa ggcagcaaag tcgtagaagt aagtttcgtt acccggatgc acaaatagta
      901 atgggtatgg aactacacca aacacgcggc caacatccat actataataa ccatagccaa
      961 tgggtgccat tctgataata tcgctcactt ttaaatcaac tttggtgtaa gtaaaatcgc
     1021 tcgaaaacac gcccttaaaa cctacggtta cattggcttg tattgccgga tattttgaac
     1081 ccaaactggt gcgtccatgt ttggtataaa caaatttttc ctgatgcatg taacgcgtgt
     1141 aaagagatat ttcgctttgt atgatgtttt ctcttacaat ggttttggca tcatccacat
     1201 agtaagcata gcttaatatg cctaaaggat ttaagttgct gttacttaaa gttaaacggt
     1261 tgctaaagcc ggcaaaccat tctttttcat agtatagttt tttaatttca gtatcggtaa
     1321 gtttattggc cgggttgcgt ctaaacaaag agctaagtat gttatcggtt tgaaatgcat
     1381 tatcgctaag ccctaattgc tgtatatcat ttttaaactg tacgccaatt aattcgcgtg
     1441 gtttgcggct aaaaaaataa tcggcaccca cgctaaattt aaagcgtgca tcgttaaaac
     1501 cgtatgccag gtaaccattt aactgaattt tttcgtggaa atcgatattg gttttt
//
LOCUS       DAOMAA010000579         1556 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_91954_length_1556_cov_2.626249, whole genome shotgun sequence.
ACCESSION   DAOMAA010000579 DAOMAA010000000
VERSION     DAOMAA010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91954_length_1556_cov_2.626249"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="PLO59_07845"
     CDS             complement(<1..375)
                     /locus_tag="PLO59_07845"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01056.1"
                     /translation="MKQFTLKIYALLLTCLLSTQMQAQSNQYLDFDGVDDFVTTNNAS
                     ALIAGAPGYTMTGWFYCNALSYGQGMMGLRATSCGFYMIMLNNGVIECRYVNSNNTLF
                     QYVTPSFTVVPQTWQHYAWVFDG"
     gene            complement(448..>1556)
                     /locus_tag="PLO59_07850"
     CDS             complement(448..>1556)
                     /locus_tag="PLO59_07850"
                     /inference="COORDINATES: protein motif:HMM:NF024152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HQV01057.1"
                     /translation="TDLAWADAHFNANGVINEPFISFKKFDNLFQGDIKKWRKLANSL
                     RLRYAIRMVNKEPALAGSIVKEIIDSNKPCFGVNDFGQLVTDHQECAAIYPFQVGFRN
                     ESKGWSFNESKDSRLGTTFWQLIATHDSADGSGIFDPRAYYFFETNNNNKWVPYPNAP
                     ATTIPTDGGIPYEYQRDVVYSIKGATCLYSPLNYYLVRDMDFQPDIFITGAEVLFLRA
                     EAYMRGIGVTKDVGMATTAFLDGIQFSLNFWQQVMQRSKLPMGTTFATNITVPSNLSF
                     ISVQNNLKFFSGTEQQQLQEIYAQQWIDFIRQPQEAWALARRTQQTPRQGTTPMQVNR
                     FSIPPIEAAYNQANWLQAIGSSGDYLNTKVWWMN"
BASE COUNT          459 a          302 c          312 g          483 t
ORIGIN      
        1 gccatcaaat acccatgcat aatgttgcca tgtttgcggt actacagtaa aactgggcgt
       61 tacatattga aacaaagtat tgtttgagtt tacataacga cattcgataa caccattgtt
      121 aagcatgatc atgtaaaaac cgcatgacgt agcgcgcaag cccatcatac cctggccgta
      181 tgacagtgca ttgcagtaaa accaacctgt catggtataa cccggggcac ctgctattaa
      241 tgccgaagcg ttgttggtgg taacaaaatc gtcaacacca tcaaagtcga gatattgatt
      301 ggattgtgcc tgcatttgtg tgcttaacaa acaggtgaga agcaatgcat aaatttttaa
      361 agtaaattgt ttcattgtta taaatattaa agttgtttgt gaaaatttaa tttgtgctat
      421 atagtaactg ttacattttt aattattcta attcatccac caaacctttg tgtttaaata
      481 atcgcctgag ctgccaattg cttgcaacca gttggcctga ttataggctg cttcgatagg
      541 tggtatgcta aagcggttta cttgcatggg tgtagtgcct tgccgtggtg tttgttgcgt
      601 acgtctggct aatgcccatg cttcttgcgg ctgacgtata aagtctatcc attgttgtgc
      661 atatatttcc tgcaattgtt gttgttcggt accgctaaaa aatttcagat tattttgaac
      721 tgaaataaat gacaaatttg aaggcactgt tatattagtg gcaaatgttg tacccattgg
      781 taatttagaa cgctgcatta cctgttgcca aaaatttaat gaaaactgaa tgccatctaa
      841 aaatgctgta gtggccatac ccacatcttt agttacacca atgccgcgca tatatgcttc
      901 ggctcgtaaa aacaaaactt cggctccggt tataaaaata tcaggctgaa agtccatatc
      961 gcgaaccaaa taataattta agggcgagta caggcaggta gcacctttta tgctgtaaac
     1021 tacatcgcgt tggtattcat aaggaatgcc accatcagtt ggaattgtag ttgcaggtgc
     1081 attggggtag ggtacccatt tattgttgtt attggtttcg aaaaagtaat aagcacgtgg
     1141 gtcaaatatg ccacttccat cagcgctatc gtgcgtggca atgagttgcc aaaaagtagt
     1201 acccaatctt gaatcttttg actcgttaaa cgaccagcct ttactttcat ttctaaatcc
     1261 aacctgaaac ggatatatgg ctgcacactc ttgatggtcg gttaccaatt ggccaaaatc
     1321 atttactccg aaacaaggtt tgttactgtc aataatttct ttaacgatgc taccggcaag
     1381 tgcaggctct ttatttacca tacgtatggc ataacgtaaa cgcaatgaat tggcaagctt
     1441 gcgccatttt tttatgtcgc cttgaaacag attgtcaaac tttttaaaac tgataaaagg
     1501 ttcgttaatt acaccgttgg cattaaaatg agcatcagcc caggctaaat cggtaa
//
LOCUS       DAOMAA010000580         1555 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_92091_length_1555_cov_2.352667, whole genome shotgun sequence.
ACCESSION   DAOMAA010000580 DAOMAA010000000
VERSION     DAOMAA010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92091_length_1555_cov_2.352667"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /locus_tag="PLO59_07855"
     CDS             <1..892
                     /locus_tag="PLO59_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020594740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HQV01058.1"
                     /translation="FSKPDWHNENYWWPYFPPKDRNPNYNPAKYPQRWNQFKDFTYKQ
                     IEELMSGYGKMDILWLDGGQVRPDSTVTEEERPWIKVPYPQEINMHRIAAMARKNQPG
                     ILMVDRSVYGYYQNYLTPEQQIPDAPLNYPWETCLTMATSWSYVANDTYKSTHQLIHT
                     LVDIVAKGGNLLLNIGPDEKGQWHNDAYMRLKEIGNWMQQNQSCIYSTTVSPPYKSDN
                     ICFTKNSKHIFAIYLAKENEPLPEQIEIKNFVPRKNATLKILGCGKATYTIKNNHTYV
                     KLPANYIKPNSHAWVLQFDN"
     gene            complement(952..>1555)
                     /locus_tag="PLO59_07860"
     CDS             complement(952..>1555)
                     /locus_tag="PLO59_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01059.1"
                     /translation="SKDAFDMFRAVLQQLEQQYREAVLKLDSRIAISYDDKGKLEVHF
                     QAANDVLVFIMQTHVFNFDPSHSIHRSSYTKDDPLRSHCGMICIYNFLTDSLKYKRLS
                     DNGIMIGRVFINKDGHYFVEGKKQLGILFNNFETEEITLDAVAKIINSAMSYALESDA
                     NVPGFDMMQVTNLQALSERSGIGFDSGKSFGFQMMHNENK"
BASE COUNT          535 a          306 c          276 g          438 t
ORIGIN      
        1 tttttcaaaa cccgactggc ataatgaaaa ttattggtgg ccttatttcc cacctaaaga
       61 caggaacccg aattataatc ctgcaaaata cccgcagcgc tggaaccagt tcaaggattt
      121 cacctacaaa cagattgagg aactcatgag tgggtatggc aagatggata ttttatggtt
      181 agatggtggt caggttcggc ccgattcaac agtaactgaa gaagaacgac cttggataaa
      241 ggtaccttat ccgcaggaaa taaacatgca ccgcatagct gcaatggcac gtaaaaatca
      301 accgggcatt ttaatggtcg acaggtcggt ttatggctat taccaaaact atttaacccc
      361 cgagcaacaa attccggatg cgccattaaa ttatccttgg gaaacttgtt taaccatggc
      421 aaccagttgg agttacgttg ctaatgatac gtataaaagt acgcatcaac tgatacatac
      481 attggttgat attgtggcca aaggcggcaa cttgcttcta aatatcggac cagatgaaaa
      541 agggcaatgg cataatgatg catacatgcg tttaaaagaa attggcaact ggatgcaaca
      601 aaaccaaagt tgtatttaca gtaccactgt ttcaccgccc tataaatcag acaatatttg
      661 ttttaccaaa aacagcaaac atatttttgc catttatctg gcaaaagaaa acgagccatt
      721 gcccgaacaa atagaaatta aaaattttgt accacgtaaa aatgccacat taaaaatttt
      781 gggctgtggc aaagcaacct atacaattaa aaacaaccat acttatgtta agttgccggc
      841 aaattatata aagcctaaca gccatgcatg ggtgctacag tttgacaatt agttaatgtg
      901 gtaaccaagg taatataatt aaaacatggt caaaaataaa tgcagttgtt tttatttatt
      961 ttcattgtgc atcatttgaa aaccaaatga tttacctgag tcaaaaccaa ttcctgagcg
     1021 ttcacttaac gcttgcagat tggttacctg catcatatcg aacccgggta cattggcatc
     1081 agactccaaa gcgtaagaca tagccgaatt tataattttt gcaacagcat ctaacgtaat
     1141 ttcttcagtt tcgaagttgt taaacaaaat gcccaattgt tttttaccct caacaaaata
     1201 atgtccatcc ttgtttataa aaacacgacc tatcataata ccgttatcgc ttaagcgttt
     1261 gtattttaac gaatcggtta aaaaattata aatacaaatc ataccgcaat gtgagcgaag
     1321 cgggtcatct ttagtatagg atgaacgatg gatactatgc gatggatcga aattaaaaac
     1381 atgcgtttgc atgataaaca ccagcacatc attagcagcc tgaaaatgaa cttccagctt
     1441 acctttatca tcataactaa ttgcaatacg tgaatctaat tttaatactg cttcgcgata
     1501 ttgttgttct aattgctgca gtactgcacg aaacatatca aacgcatctt tcgat
//
LOCUS       DAOMAA010000581         1554 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_92184_length_1554_cov_2.629753, whole genome shotgun sequence.
ACCESSION   DAOMAA010000581 DAOMAA010000000
VERSION     DAOMAA010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92184_length_1554_cov_2.629753"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="PLO59_07865"
     CDS             complement(<1..509)
                     /locus_tag="PLO59_07865"
                     /inference="COORDINATES: protein motif:HMM:NF014601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HQV01060.1"
                     /translation="MNETYNITIIGGGNLGNAIANGLLLKNFCKPAQLTVTKRNIASL
                     KNLAAKGVTITTDNVAAIKQAHVIILAIKPYQMDDMLQLLAPHIDVNTHVLCSVVTGY
                     KMEQMRKITGNIPIIRAMPNTAIAIGQSMTCLCAENASANQQAYIESLFSQLGQAVYI
                     QESLMDAATV"
     gene            612..>1554
                     /locus_tag="PLO59_07870"
     CDS             612..>1554
                     /locus_tag="PLO59_07870"
                     /inference="COORDINATES: protein motif:HMM:NF013957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HQV01061.1"
                     /translation="MKNRLLLLVSVAALTVLAYQLITASTVFTQVVENEFIKNIKQKF
                     QAHYSHQPQDRIYVQFDKSLYAAGETVWFSARIRDARWLAPSTQSDIVHVQVIAPSGN
                     ILQHQKLLIKDGITAGDYELPADASGGIYKIKAYTQWQLNDTDTGAFVYDIMVQDVML
                     PQVKCKLDFERKAYTAGDEVVALLTMNGVANEPLSNMPFTYRIKIDGKLNQEQQATTG
                     SNGSMHVRFTLPKKLETTDVNLETSIIYQGKPQTMVRSVPVVLNKISLQVFPEGGDII
                     AGCENVIAFKAVNEFNKPADVSGIVTDDAGVQVASFKS"
BASE COUNT          471 a          282 c          321 g          480 t
ORIGIN      
        1 accgttgctg catccattaa actttcctga atatatacgg cctgacctaa ctgactaaaa
       61 agcgattcaa tataggcttg ttgattagcc gaggcatttt cagcacataa acacgtcatg
      121 ctttgaccaa tagctatggc tgtattgggc atggcgcgta taatgggtat gttaccggtt
      181 atttttcgca tttgctccat tttatagcct gttaccaccg agcacaatac atgtgtattt
      241 acatcaatat gcggagctaa caattgcaac atatcgtcca tttgataagg cttaatagca
      301 agtataataa catgtgcttg ctttatggcc gctacattat ctgttgtaat tgttacccct
      361 ttagcagcta aattttttaa cgatgctata tttcttttgg ttacagtaag ttgggcaggc
      421 ttacaaaaat ttttcagtaa taaaccgttg gcaatggcat tacctaagtt gccgcctcct
      481 attatggtga tgttgtatgt ttcattcata gtgcaaagct aatgtattga gtcagtttta
      541 aaatgcagat tattttttgt ggaatttatc agtggcttgc atcccatact aaaaccataa
      601 aatattttag tatgaaaaat cgccttttgt tactcgtttc agttgcagca cttactgtat
      661 tggcttatca gttaattact gcaagtaccg tatttacgca ggttgtcgaa aatgaattta
      721 taaagaatat aaagcaaaaa tttcaggcac attacagcca tcaaccgcaa gatcgtattt
      781 acgttcagtt cgataaaagt ttgtatgcag caggcgaaac cgtatggttt agtgcgcgca
      841 tacgtgatgc acgctggctg gcgccttcaa cacaaagcga tattgtgcat gtgcaggtta
      901 ttgcgccttc gggcaatatt ttacaacatc aaaaattact tataaaagat ggcattaccg
      961 caggcgatta tgaattaccc gctgatgcaa gtggtggtat ttataaaatc aaagcatata
     1021 cgcaatggca attaaacgat accgataccg gtgcttttgt ttatgatata atggtgcagg
     1081 atgtaatgct gccacaagta aaatgcaaac ttgatttcga acgtaaagca tacactgcag
     1141 gagatgaagt tgtggcatta cttaccatga atggtgtagc taatgaaccg ctgagcaaca
     1201 tgccctttac ctatcgaatt aaaattgatg gaaagcttaa tcaggaacaa caagccacta
     1261 caggcagtaa tgggagtatg catgtgcgct ttacgttacc taaaaaattg gaaacaacgg
     1321 atgttaattt ggaaacgtca atcatatatc agggcaaacc acaaactatg gtgcgaagtg
     1381 taccggtggt gcttaataaa atttcattac aggtttttcc cgaaggtggc gatattattg
     1441 ccggctgcga aaatgtaatt gcatttaagg ctgttaatga gtttaataaa ccggccgatg
     1501 tatcgggtat tgtaaccgat gatgcaggcg tgcaggtagc atcgtttaaa agtt
//
LOCUS       DAOMAA010000582         1550 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_92592_length_1550_cov_2.917726, whole genome shotgun sequence.
ACCESSION   DAOMAA010000582 DAOMAA010000000
VERSION     DAOMAA010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92592_length_1550_cov_2.917726"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="PLO59_07875"
     CDS             <1..552
                     /locus_tag="PLO59_07875"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HQV01062.1"
                     /translation="VYAFFGVSDEEKELQFAQNLTQHGGYFLYCQNHLDFAEDFLKLC
                     ENKKWQQFLCTDKSLQNVLNQVAFKHDVNLSNNIEVYFTNAECATARTASLIFSNKQA
                     NMRAIAQAKALVVIIDIAQVVNDLKDAIQNIRLKYNNQYPAEVHALSGPFKCETQPDL
                     MQEVYVFLIEDNFVQNLSTDNLL"
     gene            590..796
                     /locus_tag="PLO59_07880"
     CDS             590..796
                     /locus_tag="PLO59_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01063.1"
                     /translation="MSDWIKIYSSEQPYKCNFVKALLDEYNIPYQELNKIDSTVVTIG
                     ELEIFVPVANEIFVRLLLTQNNLE"
     gene            789..>1550
                     /locus_tag="PLO59_07885"
     CDS             789..>1550
                     /locus_tag="PLO59_07885"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HQV01064.1"
                     /translation="MNNFITRTLSGAVFVAVLITAIWFGPITCFLLFLFIVGVSFFEF
                     NNMMLHYNVKPPLYLNTMLALLCFAIIAGNSLNFIQVSIMPIALAAMFLLLINELYRN
                     LDKPFLNIASAAIAWLYLLTPFVLLWQIGFVDGNYQPWRILGLFILQWTSDTCQYIFG
                     VTMGKHKLFERISPKKSVEGFIGGGLCCIALAYFIAPYNNCFTVTQWMVCAAIIVVFG
                     TLGDLIESMLKRSVGVKDSGNIIPGHGGILDRFDGV"
BASE COUNT          487 a          268 c          278 g          517 t
ORIGIN      
        1 gtttatgcct tttttggtgt tagcgatgaa gaaaaggaat tgcaatttgc acaaaactta
       61 acccaacatg gcggctactt tttgtattgc caaaaccatc tcgattttgc agaagatttt
      121 ttaaaactgt gtgagaataa aaaatggcaa caatttttat gtactgataa gtcattacaa
      181 aatgtattaa accaggtagc atttaaacat gatgtgaacc tatctaataa tattgaagtg
      241 tattttacaa atgctgaatg tgcaaccgca cgtacagctt cgctaatttt tagcaacaaa
      301 caagctaaca tgcgcgctat tgcccaagcc aaagctttgg tagtaattat tgatatagca
      361 caagtggtta acgacttgaa agatgccata caaaacatcc gattaaaata caataatcaa
      421 tatcctgccg aagtacatgc attaagcggg ccttttaaat gcgaaaccca acccgacctt
      481 atgcaagaag tttatgtttt tttaattgaa gataactttg tacaaaacct aagtactgat
      541 aacttattat aaaatactta ctaaaaatta aactctgtaa cattgaaaca tgagcgactg
      601 gataaaaatt tatagcagtg agcaaccata caaatgcaac tttgtaaaag cattgctcga
      661 tgaatataat ataccctatc aagagctaaa taaaatagac agtaccgtgg ttacaattgg
      721 cgaacttgaa atatttgtac ctgttgcaaa tgaaattttt gtacgcttac tcttaactca
      781 aaataacctt gaataatttt attacccgca ccctgagtgg tgccgtattt gttgctgtat
      841 taataactgc aatttggttt gggcccataa catgttttct acttttttta ttcatagtag
      901 gcgtaagttt ttttgagttt aataatatga tgcttcatta taatgttaag cctcctttat
      961 atcttaatac aatgttggca ttactttgtt ttgcaattat tgccggcaat agcctcaact
     1021 ttattcaagt ttcaataatg cccatagcgt tggcagctat gtttttacta ctaatcaatg
     1081 aattgtatcg taatttagac aaaccttttt taaacattgc atctgctgca attgcctggc
     1141 tctatttgct aacacctttt gttttactat ggcaaattgg ctttgttgac ggtaactacc
     1201 agccatggcg catacttggc ctttttatat tgcaatggac cagtgatact tgtcaatata
     1261 tttttggtgt tactatgggc aagcataaat tatttgagcg tatatcaccc aaaaaatcgg
     1321 tagaaggctt catcggtggt ggattatgtt gcattgcttt ggcctatttc atagcacctt
     1381 acaacaactg ttttacagta acgcagtgga tggtttgtgc agcaataatt gtggtttttg
     1441 gcactttagg cgacttgata gaaagtatgc tcaagcgaag tgtaggggtt aaagactcgg
     1501 gcaacattat acccggccat ggtggcatat tagacaggtt tgacggtgtt
//
LOCUS       DAOMAA010000583         1548 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_92831_length_1548_cov_2.624246, whole genome shotgun sequence.
ACCESSION   DAOMAA010000583 DAOMAA010000000
VERSION     DAOMAA010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92831_length_1548_cov_2.624246"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="PLO59_07890"
     CDS             <1..414
                     /locus_tag="PLO59_07890"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQV01065.1"
                     /translation="SNEAYNNLGMYQTNAGLLNEAVKSLNKSIELKANNKNARYNLGN
                     VYAHAGDYHTAIKQYELAIQIDPAYGDAYNNMGNSYAALKDFKNALVYYQKLLALQPA
                     NAKVTHNIGVTYMILGDSINGKNYMARAQQMQGGR"
     gene            452..>1548
                     /locus_tag="PLO59_07895"
     CDS             452..>1548
                     /locus_tag="PLO59_07895"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HQV01066.1"
                     /translation="MLQQLTLMAGIWRATLALPAGELPFNFEVKNTDKTIIEIINGAE
                     RILVDEVTYHNDSVLIQLPIFDATINAQITATGLQGVFTNNTRKTNKEIPFSAIYNNP
                     NRFVKPVIKPTYLGGKWQVTFSSGLPAQSSAIAIFNQNQGKITGTFQTPTGDYRYLDG
                     MVSGDSLWLSCFDGSHAFLFTATYSDGALRGMFYSGNHHSETWVAFNNEEAQLPNPYN
                     ITYLKPGFSTIDFSFKNTAGNTVSLSDDYFKNKVVIVQLMGSWCPNCMDETAYLSPLY
                     IAHKQKIAVVGLAFEKEGTLDYQTTQVNRLIKRYNITYPVLMAGNASKAAASAALPML
                     NGISSFPTTIVLNKKHSVAFIHTGFNGPATG"
BASE COUNT          515 a          295 c          301 g          437 t
ORIGIN      
        1 agtaacgaag cctataataa tttaggcatg tatcaaacca atgcagggtt gctaaacgaa
       61 gcggttaagt cgctcaacaa atcaattgaa cttaaagcca ataataaaaa tgcgcgttat
      121 aacttgggta atgtatatgc ccatgcaggc gattatcata cggctattaa acaatacgaa
      181 cttgctatac aaattgatcc ggcttatggc gatgcgtata acaatatggg taatagctat
      241 gcagcattaa aagattttaa aaatgcactt gtttactatc agaaattgtt agcgttgcaa
      301 cctgctaatg ctaaagttac gcataacata ggcgttacct atatgatttt aggcgatagt
      361 attaatggca aaaattatat ggcacgggca caacaaatgc aaggtggtag gtagtaacac
      421 caaataattt tcaattgcaa aaggaataat tatgcttcaa caacttacat taatggccgg
      481 tatttggcgt gctacacttg ctttaccagc aggcgaattg ccttttaatt ttgaggtaaa
      541 aaacaccgat aaaaccataa tcgaaataat taacggtgcc gaacgtattt tagttgatga
      601 agtaacctac cataatgatt cggttttaat tcagttgccc atatttgatg caaccattaa
      661 tgcacagatt actgcaacag gcttgcaagg tgtttttaca aacaataccc gtaagacgaa
      721 taaagaaata ccattttcag ccatatataa caaccccaat cgctttgtaa aacctgttat
      781 taaacctacc tatttgggtg gcaaatggca ggtaacattt agtagtggat tgccagcaca
      841 gagtagtgct atcgcaattt ttaatcaaaa ccaaggtaaa ataacgggta catttcaaac
      901 accaacaggc gattaccgtt acttagatgg catggttagt ggcgatagtt tatggctaag
      961 ttgttttgat ggtagccatg catttttgtt tactgctact tatagcgatg gtgcattgcg
     1021 cggtatgttt tatagtggta atcatcacag cgaaacctgg gtggctttta ataatgaaga
     1081 ggcacagttg cccaatccat ataacataac ctacctgaaa ccgggcttta gcaccattga
     1141 tttttcgttt aaaaatacag caggcaatac cgtttcattg tctgacgatt attttaaaaa
     1201 caaagttgtt atagtacagc ttatgggttc atggtgcccc aattgcatgg acgaaaccgc
     1261 atacctatcg ccattatata ttgcccataa acaaaaaata gccgtagtgg gtttggcatt
     1321 tgaaaaagaa ggaacgcttg attaccaaac cacacaagta aaccgattaa taaagcgcta
     1381 caacatcacc tatccggtat taatggccgg caatgcaagc aaagcagcgg catcggcagc
     1441 cttgcccatg cttaacggca ttagtagttt tcctacaacc attgtgttaa acaaaaagca
     1501 tagtgtagct tttatacaca ccggctttaa cgggccggct acaggtaa
//
LOCUS       DAOMAA010000584         1547 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_92928_length_1547_cov_2.964477, whole genome shotgun sequence.
ACCESSION   DAOMAA010000584 DAOMAA010000000
VERSION     DAOMAA010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92928_length_1547_cov_2.964477"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..887)
                     /locus_tag="PLO59_07900"
     CDS             complement(63..887)
                     /locus_tag="PLO59_07900"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HQV01067.1"
                     /translation="MHFAQNIKLLRARKKRTQDVVAQAMGFSRSTLNSYENALIVNPT
                     IEALIAFSDYFKIAIDTLLRVDMGKLSERKLLELEMGHDDFVKGTKLRVLATTVDSNN
                     IENIEMVSVRAKAGYTAGYNDPEYIRTLPTFQLPFLSAERKYRTFQISGDSMLPIPDK
                     AYVTAEYVENWNDVKDGNAYIVLTKDDGIVFKVLYNQMRSGKKLLLKSLNAVYEPYEI
                     NINEVKEIWKFINYISSQLPQPETTTDNLSTSVSNLHKEMKKISDALMRKNKRTLN"
     assembly_gap    1227..1326
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          476 a          283 c          242 g          446 t
ORIGIN      
        1 tagcagtcgg gttgtaacag tttatttact ttaagttacc tgctgctacc caacaactat
       61 ttttaattta atgtgcgttt gttttttcgc atcaatgcat ctgaaatctt tttcatttcc
      121 ttatgcaggt tgctaacact ggtggataaa ttatcggttg tggtttcggg ctgcggcaac
      181 tggctgctga tgtagtttat aaatttccat atttctttaa cctcattgat gttaatttca
      241 tacggttcat aaacggcatt aagcgatttg agcagcagtt ttttgccact gcgcatttga
      301 ttatacaata ctttaaatac aatgccatca tctttagtaa gtactatata tgcattgcca
      361 tcttttacat cgttccagtt ttcaacgtac tcggcagtaa cgtaggcctt atcgggtatg
      421 ggcagcatac tatcgccact tatctgaaag gtgcggtact tacgttcggc cgataaaaaa
      481 ggcaattgaa atgtaggcag tgtacgtata tactcagggt cgttatagcc agctgtgtaa
      541 ccggccttag cccttacact taccatttca atattttcta tgttattact atcaaccgtt
      601 gttgccagca cgcgcaattt tgtgcctttt acaaaatcat cgtgccccat ttccaactcc
      661 agcaatttgc gttctgatag cttgcccatg tcaacacgca gcagcgtatc aatagcaatt
      721 ttaaaataat cgctaaaagc tataagcgct tctatggtag ggttaacaat aagcgcattt
      781 tcgtaactgt taagggtaga gcgactaaag cccatggcct gtgctactac atcttgcgtg
      841 cgttttttgc gagcacgcaa tagttttatg ttttgtgcga agtgcatttg gctaacggtt
      901 taaattttac cacaaataaa acaacgattg tattacaatc aaataaaaga ttgtaaaaat
      961 atcataatat atttgtcgaa accaaacaac aattatatta cagccaacaa tatgactgca
     1021 atacaaacaa taacccggat ttgtaggatt gagtcaacac aaaacaacta ggatttttag
     1081 gattataaga ttttaggatt aagacagcaa aacaaaaatc ctgtaatcct gcaatcctaa
     1141 aaatcttagt tcagacaata tgaagacgat aggattaaaa acaactcaac aaaaatcctg
     1201 taatcctgta atcctaaaaa tcttagnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnttca gacaatatga agacgatagg attaagacaa cacaaaaatc ctgcaatcct
     1381 gtaatcctaa aaatcttagt tcagacaata tgaagacgat aggattaaga caacacaaaa
     1441 atcctgcaat cctgcaatcc taaaaatctt agttcagaca aattaagatt ccaggattaa
     1501 gacagcaaaa caaaaatcct gtaatcctgt aatcctaaaa atcttag
//
LOCUS       DAOMAA010000585         1547 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_92931_length_1547_cov_2.859249, whole genome shotgun sequence.
ACCESSION   DAOMAA010000585 DAOMAA010000000
VERSION     DAOMAA010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92931_length_1547_cov_2.859249"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..>1547)
                     /locus_tag="PLO59_07905"
     CDS             complement(70..>1547)
                     /locus_tag="PLO59_07905"
                     /inference="COORDINATES: protein motif:HMM:NF013442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01068.1"
                     /translation="QQQTFITENTWLLTPDFLLLLEKLQQCLVAEHQAILQYGLQLSQ
                     AMQKDFEPVVQQLKDDIEVAPFLCAPIPEWMHGLKTQIVCPANQTSQLVKLINNNERY
                     QKPDSLVLDFAYTIKPTWQAIVDGLKNAIEIAGGNAKYTQQGFASFNLDATKSNLPQV
                     IYKPRTWHQLEMNVMVGESPLPAIIFDIAALVYHASIHNNERKIVFEVALQNAYEAQW
                     LNELLHLLETVMDYDANTLKVIAGIGSVSSMLHIHAITEQLQERIVGIAADFKAKVFS
                     DLKLLRKTSKNIITERKNINQAAPGLQALAAMVSNTAQQYNVLSMAGLSVNAAGKFVD
                     YKDVDVAHYEGEIEHLFASGFDVVTLSHQEYINFALQQHKGKAISNDTLLCKHDDKIS
                     EKGLRDNIRTAITYLQAWNQGLGYAVFDNRWEDVQTFEIIRAQIYQWVRNSVHLTVSN
                     ERINSRAITTIILEEAEKLKDEINEEFAGNPISEIQTISNT"
BASE COUNT          420 a          320 c          274 g          533 t
ORIGIN      
        1 gatttaatta aaaaactcgg caagctcatc gtttaaaaac aactgctcaa cctccaatgc
       61 agcttgggtt taggtattgc ttatggtttg aatttctgat atggggttgc cggcaaactc
      121 ttcgtttatt tcatctttta atttttcggc ttcttctaaa atgatggttg ttatagccct
      181 gctgtttata cgctcattac ttacggtaag atgtactgaa ttacgtaccc attgataaat
      241 ttgtgctctg ataatttcaa aggtttgtac atcttcccat cggttatcaa atactgcata
      301 gcccaaccct tgattccatg cctgcaaata agttatggct gttctgatat tatcgcgcaa
      361 tcccttttcc gaaattttgt catcatgttt gcacaataag gtatcgttgc taattgcttt
      421 gcctttatgt tgttgcaaag caaaatttat atactcctga tgcgataatg ttaccacatc
      481 aaaacccgat gcaaataaat gctcaatttc accttcataa tgcgctacat caacatcttt
      541 ataatcaaca aacttgccgg cagcatttac gcttaaaccg gccattgaca atacattata
      601 ttgttgggca gtgttgctaa ccattgctgc caatgcttgc aagcccggtg ctgcttgatt
      661 aatattttta cgttcggtga ttatattttt agacgtttta cgaagtagtt ttaaatcgct
      721 aaaaactttt gctttaaaat cggcagctat gccaactatc ctttcctgca attgttcggt
      781 aatggcatgt atgtgcaaca tgctgctgac actgcctatg ccggcaataa cttttaaagt
      841 atttgcgtca tagtccatta cagtttccaa caaatgtaaa agctcgttca gccattgcgc
      901 ttcataagca ttttgcaatg caacttcaaa aactattttc ctttcgttat tatgaatact
      961 tgcatgatac accaaagccg ctatatcaaa aataattgcc ggcaaagggc tttcgccaac
     1021 cataacattc atttctaact gatgccatgt acgtggctta taaattactt gtggcaaatt
     1081 gcttttagtt gcatccaggt taaacgaagc aaaaccttgt tgggtatatt ttgcattgcc
     1141 gcctgcaatt tcgatagcat tttttaaacc atctactatt gcttgccatg taggtttgat
     1201 ggtgtatgca aaatcaagca ccaaactgtc gggtttttga tagcgctcat tattattaat
     1261 cagttttacc aattggcttg tttgatttgc cggacataca atttgcgttt taaggccatg
     1321 catccactct ggtattgggg cgcacaaaaa aggtgcaacc tctatatcat ccttcaattg
     1381 ttgtactacc ggttcgaaat ctttttgcat cgcttgcgat agttgcaagc catattgcaa
     1441 tattgcctga tgttcggcaa caaggcactg ctgcaatttt tccaacagta gcaaaaaatc
     1501 aggcgtgagc agccatgtgt tttctgttat aaatgtttgt tgctgtg
//
LOCUS       DAOMAA010000586         1547 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_92944_length_1547_cov_2.621314, whole genome shotgun sequence.
ACCESSION   DAOMAA010000586 DAOMAA010000000
VERSION     DAOMAA010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92944_length_1547_cov_2.621314"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="PLO59_07910"
     CDS             complement(<1..601)
                     /locus_tag="PLO59_07910"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HQV01069.1"
                     /translation="MASNKILLIDTLHQVFTSKLNAAGFEIIDATTWSLAQIINYLPA
                     CIGIGIRSRFTIDKTFINAATQLAFICRAGAGMENIDVTYAESKNIACINSPEGNRDA
                     VAEHTLGMLLMLQHKLMTANLEMRNGIWQRAENRGTELKNKTLAIIGFGNMGRAFAQR
                     LTGFDMQVIAYDPHITIETLHNVRLVDLSTVFEQADIISL"
     gene            complement(617..>1547)
                     /gene="mgtE"
                     /locus_tag="PLO59_07915"
     CDS             complement(617..>1547)
                     /gene="mgtE"
                     /locus_tag="PLO59_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013924864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HQV01070.1"
                     /translation="QVLDHIRQNGENKETLDVLYIVDESGKLIDDIRVRDFLVAPTES
                     HVSELNDGKFVALHVTDDEEVAVNIFKQHNRVALPVLDANGVLLGIITIDDILQLAEQ
                     EDTEDIQKLGAVEALEDPYMDTPYGQMLRKRAPWLIALFVGELFTASAMIHYEDAIAK
                     AVVLATFIPLIVSSGGNSGSQASTLIIRAMALGEVTLTDWWRIIKREFFMGLSLGVIL
                     AAIGFFRIAAFEAMFNSYGEAWRLVGLTVATTIIGVVTWGSILGSMLPLILKRLGADP
                     ATSSNPFVATMVDVTGLVIYFTLAVWLLKDTLL"
BASE COUNT          453 a          344 c          290 g          460 t
ORIGIN      
        1 gcaaacttat aatatcagcc tgttcaaata cggtacttaa atctaccaga cgtacattgt
       61 gcaaggtttc aattgtaata tgggggtcat aggcaataac ttgcatatcg aaaccggtta
      121 acctttgcgc aaatgcacgg cccatattgc caaaaccgat aatagcaagg gttttgtttt
      181 ttagctctgt gccgcggttt tcggctcgtt gccaaatgcc attgcgcatt tctaaattgg
      241 cagtcataag cttgtgctgc agcataagta acatacccaa agtatgctcg gcaactgcat
      301 ccctatttcc ttcgggtgag tttatgcatg caatattttt gctttcagca tacgttacat
      361 caatgttttc cataccggca ccggctctgc aaataaaagc aagttgggtg gctgcattaa
      421 taaatgtttt atcaattgta aaacggcttc taatgcctat tccaatgcat gcaggcaggt
      481 aatttattat ttgcgctaac gaccatgttg tagcatcaat aatttcgaaa cctgctgcat
      541 taagtttgct tgtaaacact tggtgcaaag tgtcaataag cagtatttta tttgaagcca
      601 tagggtgcaa aaaatattaa agcaacgtat cttttagcaa ccatacagcc agtgtaaagt
      661 aaattaccaa accggttaca tcaaccatgg ttgcaacaaa aggatttgaa gatgtggcag
      721 ggtcggcacc taatctttta agtattaaag gcaacatact gcccaatata ctgccccatg
      781 ttaccacacc aattatagtt gttgctaccg ttaagcctac caaacgccag gcttcgccat
      841 aactgttaaa catggcttca aatgcagcaa ttctgaaaaa gccaattgca gccaatatta
      901 cgcctaatga taagcccata aaaaattcgc gttttataat acgccaccaa tcggtaaggg
      961 ttacttcgcc caatgccatt gcccgtataa tcagtgttga ggcttgcgag ccactgttac
     1021 caccactcga tacaattaag ggaataaatg tagccagcac tacagcttta gcaatggcat
     1081 cttcataatg tatcatggcg ctggcagtaa ataattcgcc cacaaaaagt gcaattaacc
     1141 atggcgctct tttgcgtaac atttgcccat atggtgtatc catgtagggg tcttctaagg
     1201 cttcaacggc acctaatttc tgaatgtctt ctgtatcttc ctgttcggct aattgtaaaa
     1261 tatcatcaat ggtaattata cctaacaaca cgccatttgc atctaataca ggcaatgcca
     1321 cacggttgtg ctgtttaaaa atatttacgg ctacttcttc gtcatcggtt acatgcaggg
     1381 ctacaaattt gccatcgttt aattcgctta catggctttc ggtaggtgca actaaaaaat
     1441 cgcgcacgcg gatatcgtct atcagcttgc ccgattcgtc aacaatgtat agtacatcta
     1501 aagtttcttt attctcacca ttttgcctga tatggtcaag tacctgc
//
LOCUS       DAOMAA010000587         1543 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_93393_length_1543_cov_2.534274, whole genome shotgun sequence.
ACCESSION   DAOMAA010000587 DAOMAA010000000
VERSION     DAOMAA010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93393_length_1543_cov_2.534274"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..1525
                     /gene="aspA"
                     /locus_tag="PLO59_07920"
     CDS             119..1525
                     /gene="aspA"
                     /locus_tag="PLO59_07920"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018480014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HQV01071.1"
                     /translation="MHTRTEHDFIGSMQIPDSLYYGIQTLRARQNFNITGIPISQEPL
                     FIKALAIVKKAAAKANYDCKVFDKIICDAICYASDRLIAGDFLDQFVSDMIQGGAGTS
                     CNMNANEVIANIGLEHMGYRKGEYDFLHPNNHVNCSQSTNDAYPTAFRLALYNKIESF
                     IQAQEKLVETCKEKGEAFKDILKMGRTQLQDAVPMTLGMVFKSYATTISEDVQRMREV
                     QKLVTEINMGATAIGTGINAPEGYSKIVTQYIREISGIPVVLADDLIEATYDTGAYVQ
                     ISGTLKRCAVKLSKVCNDLRLLSSGPRCGLNEINLPQMQPGSSIMPGKVNPVIPEVVN
                     QTCFYVVGADMTITMAAEAGQLELNVMEPVIAFSLFTSLEYLTNAIQTLAEKCVKGIT
                     ANAEHCLQSVMNSVGIVTSLNPIIGYEMSSNIAKEALKTGKSVHELVVNQHKLITQSK
                     WDEIYSIENMINPRFINT"
BASE COUNT          514 a          280 c          310 g          439 t
ORIGIN      
        1 atatgcttct atgagtggca gcggcagttg cgtatatggc atcagttcaa aacctattga
       61 gataagcatc gaaaacacaa tccaattttc atccatttta aaataaaaac tcaaaaatat
      121 gcacacccgc accgaacacg attttattgg atcaatgcaa atacccgatt cattatatta
      181 tggaattcaa accttacgtg cccgtcagaa ttttaatatt accggtattc ctatttcgca
      241 ggagccttta tttattaaag cattggctat tgtaaaaaag gctgccgcta aagctaatta
      301 cgattgtaaa gtgtttgata aaataatttg cgatgctatt tgttacgcat ctgatcgctt
      361 aatagcaggc gattttttag accaatttgt gagtgatatg atacagggtg gggcaggtac
      421 cagttgcaac atgaatgcaa acgaagttat tgccaacatt ggtttagaac acatgggata
      481 cagaaaaggt gaatatgatt ttttgcatcc taataatcat gtaaactgca gccagtcaac
      541 caacgatgca taccccacag catttagatt ggcactttat aataagattg aaagttttat
      601 acaagcccag gaaaaattgg ttgaaacttg taaagaaaag ggcgaagcat ttaaagacat
      661 tttaaaaatg gggcgtaccc aattacagga tgccgtacca atgacattgg gcatggtatt
      721 taaaagttac gcaactacca ttagcgaaga tgtgcaacgc atgcgcgaag tacagaaatt
      781 ggttaccgaa attaatatgg gtgccactgc catcggaacc ggaattaatg cgcccgaagg
      841 ctattcgaaa attgttacgc aatatatcag agaaatatcg ggcatacccg ttgtacttgc
      901 cgatgattta atagaggcaa cttatgatac aggtgcctat gtgcagatat cgggtacctt
      961 aaaaagatgt gccgttaagc ttagtaaagt atgtaacgat ttacgcttat taagttcggg
     1021 cccacgttgt gggttaaacg aaattaactt gccacaaatg caacccggtt catccattat
     1081 gcccggcaaa gtaaatcctg taatacctga agtagttaac caaacttgtt tttatgttgt
     1141 aggtgctgat atgacaatta ccatggctgc cgaagccggt caactggagc ttaatgtaat
     1201 ggaacctgtt attgcattta gtttgtttac atcgctcgaa tatttaacaa atgccataca
     1261 aacactggcc gaaaaatgtg taaaaggcat cactgctaat gccgagcatt gtttgcaaag
     1321 tgtaatgaac agtgtgggta ttgtaacttc gttaaaccct attattggtt acgaaatgtc
     1381 gagcaacatt gcaaaggagg ccttaaaaac cggcaagtcg gttcatgagt tagtggttaa
     1441 ccaacataaa ctcattaccc aatcaaagtg ggacgagatt tactctattg aaaatatgat
     1501 taatccacgt tttataaata cttaaaagcg attaattaaa aac
//
LOCUS       DAOMAA010000588         1539 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_93842_length_1539_cov_3.586253, whole genome shotgun sequence.
ACCESSION   DAOMAA010000588 DAOMAA010000000
VERSION     DAOMAA010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93842_length_1539_cov_3.586253"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="PLO59_07925"
     CDS             <1..515
                     /locus_tag="PLO59_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQV01072.1"
                     /translation="ISTELFQSINPFWVVVLTPVVVWFFALLRKRNCEPSTPSKIFWG
                     LFITALSTLVMISATIVCHNGLEKSSAWWLIGCYAVVTFGELCLSPMGLSLVSKLSPP
                     RLTALMMGGWFLSTSIGNKLSGVLASMWDLYEHKAWFFSVNCIVALIAALSILAMLKW
                     LKAIAKEYGA"
     gene            664..1452
                     /locus_tag="PLO59_07930"
     CDS             664..1452
                     /locus_tag="PLO59_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01073.1"
                     /translation="MKTEKSTAKNWIPYVAGVLIAALAIFLLNRLSNSTSLNYYITLL
                     IVAFGAALALFTIQKSFASFENTSQYGTLKLGGPVVVFFMVIVGGYVLPQNNFDLTIN
                     MYDANSGAITSGAIVLNLDDKKEERLLDINGQATFKKLLSQYRNTKASIEPKIKNYPN
                     KAIEVVIPANENVLNITIEKNNDSTAIHGMVIGRDNKLQKNAQLSFLGGKVKTQSDDA
                     GNYTAVLPIADGQEAGIIIIINDSIVYNERFNVSTTGETNFYLK"
BASE COUNT          498 a          259 c          289 g          493 t
ORIGIN      
        1 taatttcaac cgaacttttt caaagtatta atccgttttg ggtagtagtt ttaacaccgg
       61 tggtggtgtg gttttttgca ttgcttcgca agcgaaattg cgaaccatca acacccagta
      121 aaatattttg gggtttattt ataacagccc tatcaacact ggtaatgatt tcggcaacta
      181 ttgtttgcca taacggatta gaaaaaagct cggcatggtg gcttattgga tgttatgctg
      241 tggtaacttt tggcgagttg tgtttatcgc caatgggttt atcgttggta agtaaattat
      301 cgccaccaag gttaactgca ttaatgatgg gtggttggtt cttatcaact tctatcggaa
      361 ataaattaag tggcgtattg gcttcgatgt gggatttgta cgaacacaaa gcctggtttt
      421 tttccgttaa ttgtattgtg gccttaattg ctgcattaag catactggct atgctaaaat
      481 ggctaaaggc aattgctaaa gaatatggcg cttagtaatt tcatagttaa tctttaactc
      541 cgattacaag cctgtttgct gctattttta ttttctattg attaaatatt tcaaaccaat
      601 gtttggtatt aaccaatgca tatagcttat atttacttgc tataaacatt aaccaaccat
      661 actttgaaaa cagaaaaatc aactgccaaa aactggatac cttatgtagc tggggtatta
      721 attgcagcct tagctatatt tttattaaac aggttaagta acagcacttc gttaaactat
      781 tatataacct tattaattgt agcctttggt gctgcactgg cattatttac aattcaaaaa
      841 tcgtttgctt cgttcgaaaa cacatcacaa tacggcacac taaaattagg tgggcccgta
      901 gttgtttttt ttatggtaat agttggtgga tatgtattgc cacaaaataa tttcgacctt
      961 actataaata tgtatgatgc aaattcgggc gctattacca gtggtgccat tgtgcttaat
     1021 ttagatgata aaaaagaaga acgcttactt gatataaatg gccaggctac atttaaaaag
     1081 ctcctaagtc aatatcgtaa taccaaagca agtatcgaac ccaaaataaa aaactaccca
     1141 aacaaagcta ttgaagttgt aatacctgcc aatgaaaatg tattaaacat caccattgaa
     1201 aaaaataacg attcaactgc tattcatggt atggtgattg ggcgcgacaa taagttacaa
     1261 aaaaatgcac aactgagttt tttaggcggc aaagtaaaaa cacaatcaga tgatgcaggc
     1321 aactacactg ccgtattacc gattgccgat gggcaggaag ccggtataat tattattatt
     1381 aacgacagca ttgtttataa cgaaaggttt aatgtaagca caaccggtga aaccaatttc
     1441 tacctaaaat aaatgaaagt tgctataagt atattttggg taatgctggt tacgatgctg
     1501 catgcgcagg aaactaccac gcttaaatta caaatgcat
//
LOCUS       DAOMAA010000589         1537 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_94114_length_1537_cov_2.738866, whole genome shotgun sequence.
ACCESSION   DAOMAA010000589 DAOMAA010000000
VERSION     DAOMAA010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94114_length_1537_cov_2.738866"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..956)
                     /locus_tag="PLO59_07935"
     CDS             complement(<1..956)
                     /locus_tag="PLO59_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HQV01074.1"
                     /translation="MSDIMSIPAPLTSKDLVTDQDVRWCPGCGDYSILKQVQTVLPEL
                     GLPREQIVFISGIGCSSRFPYYMETYGMHSIHGRATAIASGLKATRPDLSVWIVTGDG
                     DATSIGGNHFIHLLRRNFDVNVLLFNNQIYGLTKGQYSPTSEEGKITKSSPYGSVDHP
                     FNPAALAMGADGTFIARSMDRDPVHLKDMLRRANAHKGTSFLEIYQNCNVFNDGAFEI
                     FTEKSSKKQETLMIENGKPLIYGANAEKGIKLDGFKPVIVELVNTSANDLWIHDETDR
                     VKAGILARFFDDPSKADHFPRPFGVFYTENRACYEDGMNQQVA"
     gene            complement(1047..>1537)
                     /locus_tag="PLO59_07940"
     CDS             complement(1047..>1537)
                     /locus_tag="PLO59_07940"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HQV01075.1"
                     /translation="VDNALAPSNNFDPTIRVDESQNYAFQAVATNMRGFKQNIRNGNS
                     FVLINSELRVPVFQYFSQAPIRSDFIRNFMIVPFFDAGTAWTSTSPYSKDNNLNLDII
                     TQGPLTITVNKNTDPIVMGYGFGLHSRLLGYYMRADWAWSFADNEYSAKPLFNFSLSL
                     DF"
BASE COUNT          440 a          305 c          324 g          468 t
ORIGIN      
        1 gcaacttgtt gattcattcc atcttcgtaa caggctctgt tttctgtata aaaaacacca
       61 aacgggcgag gaaaatgatc ggctttcgat gggtcgtcaa agaaacgtgc taatattccg
      121 gctttaactc tgtcggtttc atcgtgtatc cacaaatcgt tggccgaggt gtttaccagt
      181 tctactataa caggcttaaa tccatctagc ttaatacctt tttcggcatt ggcgccataa
      241 ataagtggct tgccattttc aatcatcaac gtttcttgtt ttttactgct tttttcagta
      301 aaaatttcga aagctccatc gttaaacaca ttgcagtttt gataaatttc taaaaaggaa
      361 gtgcctttgt gtgcgttagc tctcctaagc atatctttta aatgaaccgg gtcgcgatcc
      421 atacttcggg caataaaagt tccgtctgct cccatggcta atgcagccgg gttaaaggga
      481 tgatcaacag agccataggg cgatgatttg gtaattttac cttcttccga agttggtgag
      541 tactgacctt tggtgaggcc ataaatttga ttgttaaaaa gcaaaacgtt tacatcaaag
      601 tttctgcgca gcagatgtat aaagtggttg ccgccaatag aagttgcatc accatcaccg
      661 gttacaatcc aaacacttaa atcggggcgg gttgctttta aaccgcttgc aattgctgtg
      721 gcacgtccat gtatgctatg cataccataa gtttccatat agtatgggaa gcgcgatgag
      781 cagccgatgc ccgatataaa aacaatttgt tcgcgtggta aacctaattc aggcagtacg
      841 gtttgcactt gttttaatat actgtagtcg ccacaaccgg ggcaccaacg cacatcctga
      901 tcggtaacta aatcctttga agtaagtggt gcaggtattg acataatatc gctcatagtt
      961 ctaatttttt tttggggtga aatatgcatt actatatgct tgcaatttta acaaacttaa
     1021 ttgattgagc aaggttttaa aacctattaa aaatcaagac ttagcgaaaa attaaatagt
     1081 ggtttggcgc tgtattcatt atcggcaaag ctccaggccc aatcggcacg catatagtag
     1141 cctaacaaac gcgagtgtaa accaaatcca tagcccatta caatggggtc ggtgttttta
     1201 tttacggtaa tggttagcgg gccttgggta attatatcta aattcagatt attatctttc
     1261 gaatagggcg aagtactggt ccatgcagtg cctgcatcaa aaaagggtac aatcataaag
     1321 tttctgataa aatcgctgcg tataggtgct tgcgaaaaat attggaatac cggcacacgc
     1381 aattcgctgt taataagcac aaagctattg ccgtttctga tgttttgttt aaaaccacgc
     1441 atgttggtag ctactgcctg aaaggcataa ttttgacttt cgtcaacccg gatggttggg
     1501 tcaaagttat tggaaggtgc caatgcatta tcaaccg
//
LOCUS       DAOMAA010000590         1535 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_94396_length_1535_cov_2.331081, whole genome shotgun sequence.
ACCESSION   DAOMAA010000590 DAOMAA010000000
VERSION     DAOMAA010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94396_length_1535_cov_2.331081"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="PLO59_07945"
     CDS             complement(<1..679)
                     /locus_tag="PLO59_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HQV01076.1"
                     /translation="MAKTQRILCWNINGIRAAWKKGFPEWALKENADIICLQETKAQP
                     DQLENDIINLGDYTSYFFSAEKKGYSGVAIYTKHKPLSVRKGLGIPLYDVEGRVIEME
                     FEKFVLYAVYFPNGGRGPERVQYKLDFYDALFQRCEMMRLKNKNIIVCGDYNTAHKEI
                     DLARPKENQKTTGFLPHERAWIDKIIDMGYTDAFRTFNQEAGQYTYWDQITRARDRNV
                     GWRIDYFM"
     gene            complement(694..1317)
                     /locus_tag="PLO59_07950"
     CDS             complement(694..1317)
                     /locus_tag="PLO59_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HQV01077.1"
                     /translation="MIPVQPPYLLQKCFGSLLWRIEEKQKVLYLTFDDGPIPQVTPFV
                     LDTLAKYQAKATFFCIGDNVRKHQQIYNDVINQKHVTANHTYNHISGWETTDFDYLKN
                     IKQAGQLISSNYFRPPYGRITPSQIRMLKHNYKIVMWDVLSMDYKLKITGMQCYMNVK
                     KHARPGSIIVFHDSLKAFARLKIALPATLKHFSDAGYIFKALPLHQL"
BASE COUNT          444 a          290 c          283 g          518 t
ORIGIN      
        1 ccataaaata atcaatccgc catcctacat ttctgtcgcg agcgcgtgta atttggtccc
       61 agtaagtgta ttgcccggcc tcctggttaa aagtcctgaa agcatccgtg taacccatgt
      121 caataatctt atcaatccag gcacgttcat gcggcaagaa acctgtagtt ttttgatttt
      181 cttttggtcg cgccaaatca atttctttat gtgccgtatt ataatcgcca caaacaatga
      241 tgtttttatt ttttaaacgc atcatttcac aacgttgaaa aagtgcatca taaaaatcga
      301 gtttatattg cacacgctca gggccacgcc caccatttgg aaagtaaact gcatagagga
      361 caaatttttc gaactccatt tcaattaccc ttccttcaac atcatatagc ggaatgccta
      421 agcctttacg caccgatagc ggtttgtgtt tggtataaat ggccacacct gagtagccct
      481 ttttttcggc cgaaaaaaag taactggtgt aatcgcctaa gttaattata tcgttttcga
      541 gttggtcggg ttgggctttg gtttcttgca ggcaaatgat atcggcattt tcttttaatg
      601 cccattccgg aaatcctttt ttccatgcgg cacgaatccc gttaatgttc cagcaaagta
      661 tgcgttgtgt tttagccatt ttaatttctg attttataat tgatgtagcg gcaatgcttt
      721 aaaaatataa ccggcatcgc taaagtgttt taatgtagca ggcaatgcaa tttttaaacg
      781 tgcaaaagct ttcaggctgt cgtgaaaaac aatgatagaa ccgggtcggg catgtttttt
      841 tacattcatg tagcattgca tgcctgtaat ctttaattta taatccatac ttaaaacatc
      901 ccacatcaca attttatagt tatgtttcag catccgtatt tgcgatggtg tgatacggcc
      961 atacggtggt ctgaaatagt tactgctaat taattgccct gcttgtttta tgttttttaa
     1021 ataatcaaaa tcggtagttt cccaaccgct gatatgatta tatgtatgat tagccgttac
     1081 gtgtttttga tttattacat cgttataaat ttgctgatgc ttacgcacgt tatctccaat
     1141 acaaaaaaaa gtagcttttg cctgatactt tgccaatgtg tcgagtacaa agggtgttac
     1201 ttgcggtatg gggccatcgt caaaagttag gtacaatacc ttttgttttt cttcgatacg
     1261 ccaaagcaaa ctgccaaaac atttttgtaa caaatagggt ggttgtacag gaatcaactt
     1321 tgttttttat aagcgtaaat aaacttacta aaatgaaatt ttaaaaagta tttgcatgtt
     1381 tacatacata catttttaca atatttgggt tattgaatta tctggcacac atatttttaa
     1441 gcggtgaaaa taacgatgta atggtaggca actttattgc cgaccactta aaaggcaata
     1501 tgcgtaatgc ttttaacggg cagataaaaa caggc
//
LOCUS       DAOMAA010000591         1533 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_94625_length_1533_cov_2.444520, whole genome shotgun sequence.
ACCESSION   DAOMAA010000591 DAOMAA010000000
VERSION     DAOMAA010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94625_length_1533_cov_2.444520"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..109
                     /locus_tag="PLO59_07955"
     CDS             <1..109
                     /locus_tag="PLO59_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009284590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HQV01078.1"
                     /translation="AAIANLAGGLVCEKVGVVPIDKKQLMLEVAKEAIL"
     gene            138..866
                     /gene="ubiE"
                     /locus_tag="PLO59_07960"
     CDS             138..866
                     /gene="ubiE"
                     /locus_tag="PLO59_07960"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011586071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HQV01079.1"
                     /translation="MLKPVTPYKHSQKSKHEQIEVMFNNIAPKYDLLNGLLSFGIDKK
                     WRKTAVSMLSSERPKHILDIATGTADFAISCMKLRPTKIIGIDISAKMLAIGREKLRK
                     LDLENHIQLMQGNSENLPFSDNHFDAVTVAFGVRNFENLQNGLAEIRRVLKPGGSLIV
                     LEFSKPKKFPVKQFYAFYSNTVLPLAGKIISRDKAAYTYLPDSIKAFPAGYAFLKELE
                     KAQFTDTIFRPLTFGITTVYKAKK"
     gene            866..>1533
                     /locus_tag="PLO59_07965"
     CDS             866..>1533
                     /locus_tag="PLO59_07965"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HQV01080.1"
                     /translation="MKQFLIIILLCMPYMATAQQSRVQNLPKYDKQKIHFGFSLGLNT
                     CNFKMEKNVSLYTYDSLYNIDNIRQGGFNLGIVTNLHMGNNFDLRFIPDLCFADRTLE
                     YTFYINNVPARSPTQKKIESTFIQLPLYVKFKSDRIGNMRAYVLAGAQYNIDMVSQAK
                     VENQDKQYVKLKRNDYGYSIGTGCDFYMEMFKLSLEVKMFNGMGNLLVEDKTIFAQSL
                     KSLRT"
BASE COUNT          527 a          287 c          269 g          450 t
ORIGIN      
        1 tgctgcaatt gctaatttag ccggagggtt ggtttgcgaa aaagttggcg tagtgcccat
       61 tgataaaaaa caacttatgc ttgaagtagc caaagaagct atactttaaa atcaataatt
      121 tacagtacta tccttatctg ttgaaacctg taacaccata caaacactcg caaaaaagta
      181 aacacgagca aattgaagta atgtttaaca acattgcacc caaatatgat ttgcttaacg
      241 gtttactatc ttttggtatt gataaaaagt ggcgtaaaac agccgtaagt atgttatcga
      301 gcgaaaggcc caaacatatt ttagatatag ctaccggtac agccgatttt gcaattagtt
      361 gtatgaaact gcgtcctaca aaaattattg gtattgatat ttcggcaaaa atgttagcta
      421 ttggacgtga aaagttgcgc aaactcgacc tcgaaaacca tattcaactg atgcaaggca
      481 attcagaaaa cttaccgttc tctgataacc actttgatgc agtaacggtt gcattcggag
      541 ttcggaattt tgaaaacctg caaaacggat tagctgaaat aagacgtgtt ttaaaaccgg
      601 gcggctcatt aattgtactc gagttttcga aacccaaaaa atttccggtt aaacaatttt
      661 atgcttttta cagcaatacc gtattacctt tagccggaaa aataatttca cgcgataagg
      721 ctgcatatac ctacctgccc gattcaatta aagcttttcc ggccggatat gcatttttaa
      781 aagaattaga aaaagcacaa tttactgata ccattttcag gccccttacg tttggtatta
      841 caaccgttta caaagcaaaa aaataatgaa acagttttta atcatcatac ttttatgcat
      901 gccttacatg gcaacggcac aacaaagtcg ggtacaaaac ctgcctaagt atgataaaca
      961 aaaaatacac tttggttttt cgctggggct caatacctgt aattttaaaa tggaaaaaaa
     1021 cgtaagttta tacacgtacg attcattata taacattgac aatatacgcc aaggcggttt
     1081 taacttaggt attgtaacca acctgcacat gggtaataat ttcgatttaa gatttatacc
     1141 cgatttatgt tttgccgacc gcacacttga atataccttt tacattaata atgtaccggc
     1201 acgctcgccc actcaaaaaa aaatcgaatc aacctttatt caattaccac tgtacgtaaa
     1261 atttaaaagc gaccgtattg gcaacatgcg tgcttatgtt ttagcaggtg cacagtacaa
     1321 tatcgacatg gtatcgcaag ccaaggtcga aaatcaggat aagcaatacg taaaacttaa
     1381 acgcaacgat tatggttatt ccataggtac cggatgcgac ttttatatgg aaatgtttaa
     1441 actttcgctc gaagtaaaaa tgtttaacgg tatgggtaat ttattggtag aggataaaac
     1501 catttttgca caatctttaa aaagtttgcg cac
//
LOCUS       DAOMAA010000592         1530 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_94963_length_1530_cov_3.009492, whole genome shotgun sequence.
ACCESSION   DAOMAA010000592 DAOMAA010000000
VERSION     DAOMAA010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94963_length_1530_cov_3.009492"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..>1530)
                     /locus_tag="PLO59_07970"
     CDS             complement(41..>1530)
                     /locus_tag="PLO59_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01081.1"
                     /translation="DKKLGLTRLVENLKVIIRQDSLQSISKMTPEEREKMVDDLIAAE
                     NKEKARLKQLADEQKLKEGEANDLKNQAMFNDNTRNRPQSQAAVGAWYFYNQSTVSFG
                     FNEFTKKWGERKLEDNWRRKNKQSTTAQIDEEESLDAPDLTAYNDSIAKLDDIKRKQA
                     YLSNVSMSDTAIEASNKKMIDAYYSIGIIYREQLKEYRLSIEAFETLLKRFPGNKYEL
                     ACYFNLYRTYKLIKDEEKSNYYKDILVNNHPESEFTKQVLNPNYWREKQKKTEILEVF
                     YENTYKAYLNRQYASVIERKNTAEELYPKNKLMPKFDYLKALSIGKIKNVGEFEVALK
                     NVVALYPGDSVSMAAQDIINYINKMQKAAVPETPKVAADTGYIYAADSTHQFMMVVEN
                     KSINVEMLKIKISNFNNKYFSMRSLTISNAFLTADKQYILVKSFTNATEAHAYYDALV
                     NDEDVLNGVDESKLNTCTITPANFSKLFIKKDLAGYLDFFDQKYQ"
BASE COUNT          380 a          287 c          288 g          575 t
ORIGIN      
        1 agtattgcca caaacaattt gaataaatag ttgatttttt ttactgatac ttttgatcaa
       61 aaaaatcgag ataaccggcc aagtcttttt ttataaaaag tttggagaaa tttgccggtg
      121 taatggtgca ggtgttgagt ttactttcgt caacgccatt tagtacatct tcgtcattta
      181 ccaaagcatc ataataggca tgggcttcgg tggcattggt aaatgatttt accagtatat
      241 attgtttatc ggcagttaaa aatgcattgc taatggtaag gcttcgcatg ctgaaatact
      301 tgttgttgaa attgcttatt ttaattttaa gcatctctac attgattgat ttgttttcga
      361 caaccatcat aaactggtgg gtgctgtcgg cagcatatat gtaacctgta tcggccgcta
      421 ctttaggtgt ttcgggcaca gcagcctttt gcattttgtt tatgtagtta ataatatcct
      481 gagcagccat cgaaaccgaa tcgcccggat acagtgctac tacatttttt aatgctactt
      541 caaactcgcc tacattttta attttcccga ttgataaggc ctttaaataa tcgaacttag
      601 gcatgagttt gtttttagga tacaactctt cggccgtgtt tttacgttca ataacactgg
      661 catattgccg gtttaaatat gctttgtatg tgttttcgta aaacacttcc aatatttcag
      721 ttttcttttg tttttcgcgc caatagttgg ggttaagtac ttgtttggta aactccgact
      781 cggggtggtt gtttaccaaa atatctttat agtaattgct cttttcttcg tctttaatca
      841 gtttataagt acggtacaaa ttgaagtagc aggccagttc gtatttattg cccggaaatc
      901 tttttaataa agtttcaaat gcttcaatac ttaaccgata ctcttttaat tgttcgcggt
      961 aaataatgcc aatgctgtaa taggcgtcaa tcatcttttt attgctggct tcaatggccg
     1021 tatcgctcat actaacatta ctcagataag cctgcttgcg ttttatatca tctaacttgg
     1081 caatcgaatc gttgtaggct gtaaggtcgg gtgcatctaa actttcttct tcatcaattt
     1141 gggccgtggt gctttgtttg ttttttctgc gccaattatc ctcgagttta cgctcgcccc
     1201 attttttggt aaactcatta aatccaaaac ttacggttga ttggttgtaa aagtaccatg
     1261 ccccaacagc agcctgactt tgcggcctgt tgcgtgtatt gtcgttaaac atggcttggt
     1321 tttttaaatc gttggcttcg ccttctttta gcttttgttc atcggccaat tgtttaaggc
     1381 gtgctttttc tttgttttcg gcagctatta aatcatcaac cattttttcg cgttcctcag
     1441 gggtcatttt actaatggat tgcaaactat cctggcggat aataactttt aagttctcaa
     1501 ctaagcgggt taagcctaac tttttatctt
//
LOCUS       DAOMAA010000593         1526 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_95439_length_1526_cov_3.414684, whole genome shotgun sequence.
ACCESSION   DAOMAA010000593 DAOMAA010000000
VERSION     DAOMAA010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95439_length_1526_cov_3.414684"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1121
                     /locus_tag="PLO59_07975"
     CDS             <1..1121
                     /locus_tag="PLO59_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018343081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HQV01082.1"
                     /translation="FVNGQHTTQGGTHQAAFREGIVKAAREYFDKDFDANDVRSSIMA
                     AVSIRIQEPVFESQTKTKLGSQTTEPNGNGQSLRNWIVDFIKKELDNYLHKNLEMAEA
                     WRKKILQSERERKDIAGIKKLANERAKKANMHNKKLRDCRVHLDSKHTDKYESTIFIT
                     EGDSASGSITTSRDVSTQAVFSLRGKPLNCFGLTKKVVYENEEFNLMQNALNIEDGID
                     GLRYNRIVVATDADVDGMHIRLLLLTFFLQFFPDLVKAGHVYILQTPLFRVRNRKETI
                     YCYNESERIKAIEKLGAKPEITRFKGLGEISPDEFKKFIGKKMRLEPVEMGDHTIIHQ
                     LLDYYMGKNSTERQEFIINNLRIEEELPPLDNAVLALN"
BASE COUNT          561 a          282 c          290 g          393 t
ORIGIN      
        1 cttttgtaaa cggacaacac actacccaag gtggcacaca tcaggcagct tttcgcgagg
       61 gtattgtaaa agctgcacgc gaatattttg ataaagactt tgatgccaat gatgtacgta
      121 gttcaattat ggctgctgta tcaatcagaa tacaggagcc cgttttcgaa agtcagacaa
      181 aaacaaaatt aggttcgcaa accaccgaac ccaatggtaa cggacaaagc ctgcgcaact
      241 ggatagttga ttttataaaa aaggaattag ataactacct gcataaaaac ttagaaatgg
      301 ccgaagcctg gcgcaaaaaa atattgcaga gcgaacgcga gcgaaaagat attgccggca
      361 taaaaaaatt agcaaacgaa cgtgccaaga aagccaatat gcacaataaa aaattgcgcg
      421 attgcagagt acatttagat agcaaacaca ccgacaaata cgaaagcacc atttttatta
      481 ccgaaggcga tagtgccagt ggcagcatta ccacctcgcg cgatgtgagt acacaagctg
      541 tttttagttt aagaggtaag cccttaaatt gttttgggtt aaccaaaaag gtagtgtacg
      601 aaaacgaaga atttaacctg atgcaaaatg cattaaacat tgaggatggc attgacggcc
      661 tacgttataa cagaatagta gttgctaccg atgccgatgt agatggcatg catatacgct
      721 tgttgctact cacatttttt cttcaatttt ttccagattt agttaaagcc ggccatgtat
      781 atattttaca aacacctttg ttcagagtac gtaatagaaa agaaaccatc tattgttata
      841 atgaatcgga acgtataaaa gctattgaga aattaggcgc aaaaccagaa ataacaaggt
      901 ttaaaggact aggcgaaatt tcgcccgatg agtttaaaaa atttattgga aaaaaaatgc
      961 gcctcgaacc ggttgaaatg ggcgaccata ccatcatcca tcaattgctt gattattaca
     1021 tgggtaaaaa ctctaccgaa cggcaggagt ttatcatcaa caatctgcgt atagaagagg
     1081 agttaccacc acttgataat gcagttcttg cattaaatta atcagcgcta acaacaatag
     1141 gcaaaatgat taaaagcctg caagccgtaa aaccttctaa tccatagtta tatccaacaa
     1201 aagcaactga ttaaattttc aattgcaaag ttttgtcagc aaatgcactc aatcaaaagc
     1261 aatctgagaa ttagcaatca aaaaacgcat gggattatta acaaagccga tttaaaaatt
     1321 gttactgaaa catggcgttc aaacacatta taattaacca ttaaactaac ttgcatacaa
     1381 taaatttgca ttaaaaaata ttacaaatgg agcccggtaa aaaagtaaat cacgttgatg
     1441 acatgtacca gaattggttt ttagaatatg catcctatgt gataatggag cgcgctgttc
     1501 cttatcttga ggatggttta aaaccg
//
LOCUS       DAOMAA010000594         1522 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_95963_length_1522_cov_2.839809, whole genome shotgun sequence.
ACCESSION   DAOMAA010000594 DAOMAA010000000
VERSION     DAOMAA010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95963_length_1522_cov_2.839809"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..857
                     /locus_tag="PLO59_07980"
     CDS             <1..857
                     /locus_tag="PLO59_07980"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HQV01083.1"
                     /translation="GKKLIGTVTLAESAFTAESLSIETLDGTIKANGQIDASQPDLQI
                     ALHANFDHIDATKTFYAFNNFGQNALTADNIKGEITAQVQLTSTWTKQLVLENNSLYS
                     NCKIKITNGALINFKPMMSLGRYLKEADLNKVTFDTLNNTIEIKNGTIYIPLMHINSS
                     ALKLKASGSHTFDNMVDYHIQLGLSQLLNKKIVNNQTEFGQIEDDGLGNWQLYLKMTG
                     NIKDPKFAYDKKSVKQHISKQVATEKQTVKDLFKKEFGWFKGDSTQAKNPKPQKQKEA
                     VQIDYDDE"
     gene            933..>1522
                     /locus_tag="PLO59_07985"
     CDS             933..>1522
                     /locus_tag="PLO59_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HQV01084.1"
                     /translation="MNIYTQKQRWKILLLLAALLIGMLSLWYTNNLVKVLQKEEKKRI
                     ELWAEALTLTSRMDVNAGDLTLTLDVIKSNTTIPVIRTDKNFEIIDYINLDTAKVNRD
                     TSYLRKEIEEMRLQHEPIKSIYAENECDYIFYKDSAILNKLRYYPLFQLGIIALFLLV
                     SYLAFSTSRKAEQNQVWVGMAKETAHQLGTPLSSLSA"
BASE COUNT          579 a          254 c          263 g          426 t
ORIGIN      
        1 aaggaaaaaa attaattggc accgtaactt tagccgaaag cgcttttact gccgaatcgc
       61 taagtatcga aacattagat ggcaccatta aagcaaacgg ccagattgat gcttcgcaac
      121 ctgatttgca aatagcgttg catgcaaact ttgatcatat tgatgcgact aaaacttttt
      181 atgcatttaa taattttggt caaaacgctt taactgccga taatattaaa ggtgaaatta
      241 ctgctcaggt acaattaaca tcaacctgga caaaacaatt ggttttagaa aataacagcc
      301 tatatagtaa ctgtaaaatt aaaattacca atggtgcttt aataaacttt aagcccatga
      361 tgtcgttagg acggtattta aaagaggccg atttaaataa agtaactttc gacacattaa
      421 ataatacaat cgaaattaaa aatggcacca tttatatacc gcttatgcat attaattctt
      481 cagctttaaa actaaaagca agtggtagcc atactttcga taacatggta gattatcata
      541 tacaattggg cctatcgcaa cttttaaata aaaaaattgt aaacaatcaa acagagtttg
      601 ggcaaattga agatgatggt ttgggtaatt ggcagcttta tttaaaaatg accggcaaca
      661 taaaggatcc taaatttgct tatgataaaa aatccgtgaa acaacatata agcaaacaag
      721 tagccacaga aaaacaaacg gttaaagatt tatttaaaaa ggaatttggt tggtttaaag
      781 gcgatagtac ccaggcaaaa aatcccaaac cacaaaagca aaaggaagca gttcaaattg
      841 attatgatga tgaataaaag tatcgggctg attaattatc gaacagccca caaaaattaa
      901 tttataattg taaaataata gcgcatcagg ttttgaatat atacacgcaa aaacaacggt
      961 ggaaaatact tttattgtta gctgccctgc ttataggcat gctaagttta tggtacacga
     1021 ataacttagt aaaggtttta caaaaagaag aaaagaaacg tattgaattg tgggccgaag
     1081 cattaaccct tacatcgcgc atggatgtaa atgcaggcga tttaacccta acactggatg
     1141 ttataaaaag caataccacc atacctgtaa tcagaacaga taaaaatttc gaaataattg
     1201 attacatcaa tttagataca gcaaaagtaa atcgcgatac atcttatctt cgaaaggaaa
     1261 tagaagaaat gcgcttgcaa catgagccca ttaaatcaat atatgccgaa aacgaatgcg
     1321 attatatttt ttataaagac tcagccattt taaataaact gcgttattac ccgttgtttc
     1381 agttaggcat tatagccttg tttttattgg taagttattt ggcattcagt acctcacgta
     1441 aggctgagca aaaccaggtt tgggtgggca tggccaaaga aacagcacat caattaggca
     1501 cacctctttc gtcactatca gc
//
LOCUS       DAOMAA010000595         1520 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_96215_length_1520_cov_2.665529, whole genome shotgun sequence.
ACCESSION   DAOMAA010000595 DAOMAA010000000
VERSION     DAOMAA010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96215_length_1520_cov_2.665529"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="PLO59_07990"
     CDS             <1..316
                     /locus_tag="PLO59_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01085.1"
                     /translation="IEIKPLKIYKHIAYFGQVPFLFYILHFYIIHLALMCRFLLTGHS
                     FNEHTPDIWGITFKFQLVGVGDSLALVYVYWFTVIALLYPVCKWFALYKQKNKHWWLS
                     YL"
     gene            335..1489
                     /locus_tag="PLO59_07995"
     CDS             335..1489
                     /locus_tag="PLO59_07995"
                     /inference="COORDINATES: protein motif:HMM:NF025059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HQV01086.1"
                     /translation="MNTKPLITILENALQQNVLVRVKILPDRQGNLKYIQIKPTLIKS
                     KLHLSFTYRNATNDVVKNYILAEAFVQIINNIHVYKQVYVFTTQADFIAEITNNEIHI
                     KQQKPTFIKTTDLKHNTPKKYPISEQAPYLRLLQVTGQNNQVLSNAYNKFKQINQYIS
                     LLQPLFTQTGNQPIHIADMGCGKGYLTFALYDYLQGVGYNPHITGIELRDTLVEQCNQ
                     YAVQCNYTNLTFKQGSIVNATLTNTQMLIALHACDTATDEALFTGIKNKCQHIVVAPC
                     CHKQIRQNMHQNDAVLKYITSYGIYKERMAETITDVLRMLYLNACGYATKAVEFVSDA
                     HTPKNVMIIASKNIEAKHNVNDLIAETKNMYGISSHALEEMLTANNLLEI"
BASE COUNT          559 a          279 c          246 g          436 t
ORIGIN      
        1 tatagaaata aaaccactta aaatttataa acacattgca tactttggcc aggtaccgtt
       61 tttgttttac atacttcact tttacattat ccaccttgca ttgatgtgcc ggtttttatt
      121 gacaggtcat tcatttaatg agcatacgcc tgacatttgg ggcatcactt ttaaatttca
      181 gttagttggt gttggcgatt cgttagcctt agtgtatgta tattggttta ctgtaatagc
      241 gttgttatac ccggtttgta aatggtttgc attgtataaa caaaaaaaca aacattggtg
      301 gcttagttat ttatagataa atgcctgctt gtaaatgaat acaaaaccgc ttattacaat
      361 tttagaaaat gcactgcaac aaaatgtatt ggtaagggta aaaatattgc ctgatcggca
      421 aggcaactta aaatatatcc aaataaaacc aacgcttata aaaagcaaat tgcacctgag
      481 ctttacttat agaaatgcca ctaacgatgt agtaaaaaat tacatactgg ccgaagcttt
      541 cgtacaaata atcaataaca tacatgttta caagcaggta tatgtattta caacccaagc
      601 cgactttatt gctgaaataa ccaacaatga aattcacatc aaacaacaaa aaccaacctt
      661 tataaaaacc accgacctga aacacaatac cccaaaaaaa tatcccatat cagaacaggc
      721 gccttattta aggttgttac aggttaccgg ccaaaataat caggtattaa gcaatgcata
      781 caataagttt aaacaaataa atcaatacat aagtttattg caaccattgt ttacgcaaac
      841 gggcaatcaa cccatacata ttgccgatat gggttgtgga aaaggatacc taacctttgc
      901 cctatacgat tatttgcaag gtgttggtta taatccgcac ataacaggca ttgaactaag
      961 ggacacatta gttgaacaat gcaaccaata tgcagtacaa tgcaattaca ccaatttaac
     1021 ttttaagcaa ggcagtattg taaatgctac gcttactaac acacagatgc ttattgcttt
     1081 gcatgcatgc gatacagcaa ctgatgaagc cctatttaca ggtataaaaa acaaatgcca
     1141 acacattgtg gttgcacctt gttgccataa acaaattcga caaaacatgc atcagaacga
     1201 tgctgtatta aaatacatta caagttatgg catttataaa gaacgtatgg ccgaaaccat
     1261 aacggatgtt ttgcgcatgt tgtatttaaa tgcatgcggc tatgcaacaa aagctgttga
     1321 atttgtatcg gatgcccata cgcccaaaaa cgtaatgatt attgccagca aaaacattga
     1381 agcaaagcat aatgttaacg acttaatagc agagactaaa aatatgtatg gcataagcag
     1441 ccatgcatta gaagaaatgt taacagccaa caatttattg gaaatttgaa accataaatt
     1501 atgttgacaa acattaaatc
//
LOCUS       DAOMAA010000596         1519 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_96335_length_1519_cov_2.911885, whole genome shotgun sequence.
ACCESSION   DAOMAA010000596 DAOMAA010000000
VERSION     DAOMAA010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96335_length_1519_cov_2.911885"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PLO59_08000"
     CDS             complement(<1..233)
                     /locus_tag="PLO59_08000"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002980643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HQV01087.1"
                     /translation="MINRSVKNAEEAIAGITDNMTLLVGGFGLCGIPENCINALVQSG
                     VKGLTCISNNAGVDDFGLGLLLQNKQIKKMISS"
     gene            complement(260..>1519)
                     /locus_tag="PLO59_08005"
     CDS             complement(260..>1519)
                     /locus_tag="PLO59_08005"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HQV01088.1"
                     /translation="FAEEAVKEHLTDLQKQFNDHWKGRIPWAEHPEIITNAMKQSDRY
                     RELKEGGATDAEIKKVFDTKIKMQLFTWQGERDTTMSPLDSIKHYKKMLQTGFMAMEP
                     QTGYVRAWVGGNDYKYFKYDHVKEGKRQVGSTFKPFVYTLAMQEGYSPCYKVPNVKVT
                     IETPSGPWTPDNSDNKYGGMLSLKEGLAESVNTVSAYLMKQFGPKAVVDIAHRMGIQS
                     ALEPVPAICLGTADLSVFELIGAYGTFANKGVWTEPVYITRIEDNNGVVLQEFIPKKI
                     EAISEETAYLMINAMQGATLYGTSARLRGSKYGFTNAIAAKTGTTQNNSDGWFVGMVP
                     NLVAGCWVGCEDRSVHFRSTSLGQGANMALPIWGLFMKKCYADKTLKVANTDFEKPLT
                     KLSVEIDCSKYQEQKSMDGNDDFGINN"
BASE COUNT          403 a          339 c          279 g          498 t
ORIGIN      
        1 tatgacgaaa tcattttttt tatctgtttg ttttgtaaaa gcaaacccaa accaaaatca
       61 tctacaccgg cattgttcga aatacatgtt agccctttta ccccgctttg caccagtgca
      121 tttatacaat tttcgggtat gccacataaa ccaaaaccac caaccagcaa ggtcatatta
      181 tcggttatgc cggcaatggc ttcttcggca ttttttacag aacggtttat catcgtattt
      241 aatttttaaa tcaattttat taattattaa tgccaaaatc atcattacca tccatgcttt
      301 tttgttcctg atatttggag caatcaattt caactgacaa tttggttaaa ggtttttcaa
      361 aatcggtatt ggcaacttta agtgttttat cggcataaca ttttttcata aacaagcccc
      421 atataggtaa agccatattg gctccctgtc ccaaacttgt acttcggaaa tgcacagagc
      481 ggtcttcgca acccacccaa caaccggcta ctaaatttgg caccatgcca acaaaccatc
      541 catcgctgtt attttgtgta gtgccggttt tggcagcaat ggcattggta aagccatatt
      601 tgctcccacg caggcgggca ctggtaccat acaaggtagc gccttgcatg gcattaatca
      661 tcagataggc tgtttcttcg ctaatggctt caattttttt aggaataaat tcctgaagca
      721 ccacaccgtt attgtcttca atgcgtgtta tataaacagg ctctgtccaa acacctttgt
      781 ttgcaaaagt gccataagca ccaattaatt cgaaaaccga taaatcggct gtgcctaaac
      841 aaatagctgg caccggctct aatgcacttt gtatgcccat gcgatgggct atatccacca
      901 cggctttggg accgaattgt ttcattaaat aagccgaaac ggtgtttaca ctttcggcca
      961 aaccttcttt aagcgaaagc ataccaccat acttattatc gctgttatca ggtgtccagg
     1021 ggcctgatgg tgtttcgatg gtaactttta cattggggac tttgtaacag ggtgagtatc
     1081 cttcttgcat ggccagggtg taaacaaatg gcttaaaggt tgaacccact tgtcgtttac
     1141 cttctttaac atgatcgtat ttaaaatatt tgtaatcatt acctcctacc caggctctaa
     1201 cgtaacctgt ttgtggttcc attgccataa aacctgtttg caacattttt ttataatgct
     1261 taatggaatc taatggcgac atggtggtat cgcgttcgcc ttgccatgta aacagttgca
     1321 ttttaatttt ggtatcaaat acctttttaa tttcggcatc ggttgcgcct ccttccttta
     1381 attcgcgata acggtcgctt tgcttcattg cattggtaat aatttcgggg tgctcggccc
     1441 agggtatgcg ccccttccaa tgatcgttaa attgtttttg caaatcggtt aaatgttctt
     1501 ttacggcttc ttcggcaaa
//
LOCUS       DAOMAA010000597         1519 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_96360_length_1519_cov_2.400273, whole genome shotgun sequence.
ACCESSION   DAOMAA010000597 DAOMAA010000000
VERSION     DAOMAA010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96360_length_1519_cov_2.400273"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="PLO59_08010"
     CDS             complement(<1..287)
                     /locus_tag="PLO59_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HQV01089.1"
                     /translation="MKNIKKYYTIPVPPTQLYQALTNQFTIELWTGGDAVMQAVADTE
                     FSLWDGSIVGRNLQFETDKLIKQQWYFGDEETPSIVTIKLHADKKGTSVEL"
     gene            complement(309..>1519)
                     /locus_tag="PLO59_08015"
     CDS             complement(309..>1519)
                     /locus_tag="PLO59_08015"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQV01090.1"
                     /translation="DTATTTLSQYETKRTYVDNEWTYLTAKGAMHKLRGRYYYTGNKN
                     NTNQEAFGHVYFAEYQFQKKVTSWLTTTAGLLHQYNKVTSQLYGNHTSRNVAGYVQAD
                     ATYKRFNFSLGGRVEWNKIDTVTSDATPVFRAGLNYKVANGTNIRASYGQGYRFPSIA
                     EKFVQTEVSSIVIFPNDSLTSEKGWSAEWGLQQAFAISNWKAYADVALFVNRYTNMME
                     FTFGIYGPPAPPLFGAGFQSQNIGNTLIKGIDFTLAGQGNIGMLPLQVLAGYTFIDPT
                     YTDFDAAHDTLYNSSTKNILKYRYQHMFKSDVQLSIKKFSVGFSTRYFSFMENIDKPF
                     ELLIPGVQHYRKNNHQGDWIVDARIAYAFDKNVSVAFITKNIFNNVYVSRPADVQPVR
                     TFTLQLVLKY"
BASE COUNT          425 a          306 c          297 g          491 t
ORIGIN      
        1 aacaattcaa ccgatgttcc ttttttatca gcgtgcaatt taatggttac aattgacggt
       61 gtttcttcat cgccaaaata ccattgttgt tttattagct tatcggtttc gaactggagg
      121 ttacggccta caatgcttcc atcccatagc gaaaattctg tatcggcaac tgcctgcata
      181 actgcatcgc caccggtcca tagttctatg gtgaattgat tggttaatgc ctgatataat
      241 tgtgttggtg gtaccggaat ggtataatac ttttttatgt ttttcatgat aataattaat
      301 ttctgatgtt agtatttcaa aaccaattgc agtgtaaaag tgcgtaccgg ttgtacatcg
      361 gccggccttg atacatacac attattaaat atgtttttag ttataaaagc tacgcttaca
      421 tttttatcaa aggcataagc aatgcgggcg tccacaatcc aatcgccttg atggttgttt
      481 ttgcgataat gttgcactcc gggtatcagt aattcgaaag gcttgtcaat gttttccata
      541 aagctaaaat agcgtgtgct gaaacctaca ctaaactttt ttatggataa ctgcacatcg
      601 cttttaaaca tgtgctggta gcgatacttt aaaatatttt tagttgaact gttgtaaagt
      661 gtatcatgcg cagcatcaaa atcggtataa gtaggatcaa taaaagtata gcctgctaaa
      721 acctgtagtg gcaacatacc tatgttaccc tgccctgcta aagtaaaatc aatacctttt
      781 attaaagtgt tgccaatgtt ttgcgactga aaacctgcac caaataatgg tggtgccggt
      841 gggccatata taccaaatgt aaactccatc atattggtat atcggttaac aaacaaagcc
      901 acatcggcat aggctttcca attacttatt gcaaatgcct gttgcaaacc ccactcggca
      961 ctccagcctt tttcgctggt taaactatcg ttcggaaaaa taacaatgct gctaacttcg
     1021 gtttgcacaa atttttcggc aatacttggg aaacgatacc cctggccgta tgaagcacgt
     1081 atattagtac cgttagctac tttataattt aaaccggcac gaaacaccgg tgtggcatcg
     1141 ctggttacgg tatcaatttt attccactca acccggcctc ctaatgaaaa attaaaccgc
     1201 ttgtaggttg catcggcttg cacataacct gcaacatttc tggatgtgtg attgccatat
     1261 aactgactgg ttactttatt gtattgatgc aacaagccgg ctgtagtggt tagccaggag
     1321 gttacctttt tttgaaactg atattcggca aaataaacat gtccaaacgc ctcctgattg
     1381 gtattgtttt tattacccgt ataataatag cggccacgca atttatgcat tgcacctttg
     1441 gctgtcaggt acgtccattc attatcaaca taggtgcgtt tggtttcata ttggcttaag
     1501 gtggtggtag cggtatcgg
//
LOCUS       DAOMAA010000598         1517 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_96553_length_1517_cov_3.755130, whole genome shotgun sequence.
ACCESSION   DAOMAA010000598 DAOMAA010000000
VERSION     DAOMAA010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96553_length_1517_cov_3.755130"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            278..1252
                     /locus_tag="PLO59_08020"
     CDS             278..1252
                     /locus_tag="PLO59_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQV01091.1"
                     /translation="MNDKTALITGAAGFLGSHLCDRFIAEGCKVIAMDNLITGDLRNI
                     EHLKKHPKFTFVLHDVSKYVAIDEHLDYILHFASPASPIDYLKIPIQTLKVGSLGTHN
                     LLGLALHKKATILVASTSEIYGDPLVHPQPEDYWGNVNPVGPRGVYDEAKRFQEAMTM
                     AYHTAKGLDTRIVRIFNTYGARMRLNDGRALPAFFGQALRGEDITVFGDGSQTRSFCY
                     VDDLIEGIVRLLHSGYHQPVNIGNPDEVTIARFAQEIIALTGSKQKVIFKPLPIDDPK
                     QRQPDISRARKILKWQPQVSRHEGLKTTYNYFKNLPTDEWFKPVHDFQ"
     gene            1318..>1517
                     /locus_tag="PLO59_08025"
     CDS             1318..>1517
                     /locus_tag="PLO59_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HQV01092.1"
                     /translation="MSFQAHPTAVIDPGCTIGTGTKIWHFSHIMPNCIIGQNCNIGQN
                     VVVSSDVTLGNNVKVQNNVSIYT"
BASE COUNT          491 a          292 c          296 g          438 t
ORIGIN      
        1 ttgccgaaaa aaccaacaga actaaaatac ttaacagcag taaaataaca tcaaatgtaa
       61 aaggcttgaa aaccgaaagg ggaatatgtc gtagtaaact atccgggtcc acgtattaac
      121 ttattaagcg cacaaatata taaactacct tgttttctgt ttgtatttaa aatataacag
      181 taagatggat gcatggcatc aagaatagtt tattattttt ctactactaa tttttaaatt
      241 ttatttaagg cctcaaattt ttttattgct tatggaattg aatgataaaa ctgcattaat
      301 aacaggcgct gccggctttt taggttcgca tttatgcgat aggtttattg ccgaaggctg
      361 taaagttatt gccatggata atttaattac aggcgacttg cgcaatattg agcatctgaa
      421 aaaacatcca aagtttactt ttgttttgca cgatgtgagc aagtatgtag ctattgacga
      481 acatctcgac tatatacttc attttgcatc accggcaagc cccattgatt atttaaaaat
      541 accaattcaa acacttaagg tagggtcgct gggcacccat aatttattag ggcttgcatt
      601 gcataaaaaa gccaccatac tggtagccag tacgtccgaa atttatggcg accctttagt
      661 acatcctcaa cccgaagatt attggggtaa tgtaaacccc gtggggccgc gtggcgtgta
      721 tgacgaagcc aaacgttttc aggaagccat gactatggct tatcacaccg ctaagggatt
      781 agatacgcgc attgtgcgta tttttaatac ttatggtgca cgcatgcgtt taaacgatgg
      841 cagggcattg ccggcttttt ttgggcaggc tttacgtggc gaagacatta ccgtatttgg
      901 agatggcagc caaacccggt cgttttgtta tgttgacgat ttaattgaag gcattgtgag
      961 gcttttacac tcggggtatc accagccggt taatattggc aaccctgacg aagtaaccat
     1021 tgcccggttt gcacaagaaa ttatagctct taccggcagt aaacaaaaag taatttttaa
     1081 acccttgccc attgacgacc caaaacaacg gcaaccggat attagccgcg cccgtaaaat
     1141 tttaaaatgg caaccacaag taagcaggca tgaaggatta aaaactacat acaattattt
     1201 taaaaacctg ccaactgacg agtggtttaa gcccgtacac gattttcaat agatatctat
     1261 aaaataagct ataagctgta aagcatttat atttttgcgt tttaaaacaa acataacatg
     1321 agcttccaag cacaccctac agccgttatc gaccccggct gtaccatagg aacaggtaca
     1381 aaaatctggc atttcagcca tataatgcct aattgtataa ttggtcaaaa ctgtaatatt
     1441 ggtcaaaatg tggtggtaag tagcgatgtt accttaggca ataacgtaaa agtacaaaac
     1501 aacgtaagta tttatac
//
LOCUS       DAOMAA010000599         1517 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_96568_length_1517_cov_3.128591, whole genome shotgun sequence.
ACCESSION   DAOMAA010000599 DAOMAA010000000
VERSION     DAOMAA010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96568_length_1517_cov_3.128591"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            201..>1517
                     /locus_tag="PLO59_08030"
     CDS             201..>1517
                     /locus_tag="PLO59_08030"
                     /inference="COORDINATES: protein motif:HMM:NF024882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HQV01093.1"
                     /translation="MSNGNKFKSKQTDDLPEEKPVKKRSSKTTPAEPVQTEAISNNYQ
                     KPLRWLGLSCMLLSVFLLIPFFSYLFTHAADFDKVNQTSWLSLLGNADVMVDNWLGKL
                     GAIVAHAFIYNGFGIAAAVFILLSFLLGFKLVFKVDLLPWPKTLKYSFFALLFLSITL
                     GYLMGKHSHLLWLGGVYGYQMSTWLNATIGVLGTGLLLVFAAILFVASVFKYSYQLPV
                     SAQKAAPESVVENTHAPAAGNALRDEVSTPIVMKVKDEIALEIEDPVEPEIFNSAIEA
                     DEKEDDEADLEMEVLMPELKPVSLEVTPNQQPIENENAIAAVPMDIALPEDEAVTAEN
                     LLAKMGKYDPTLDLSAYKYPTIDLLEDYQSRKTRHTPEEIKAELEANKNKIVVTLGHY
                     NIEIEKIKATIGPTVTLFEIVPKAGIRISKIKNLEDDIALNLMAYGI"
BASE COUNT          486 a          256 c          301 g          474 t
ORIGIN      
        1 aaaagttacg aaagaagcat taaataagat ttggcaacaa gcataaaaac tgtggttttc
       61 gataattaga tgcattttat aagcagtaat aaatgctttt tccaaacaat gtactgttag
      121 taagtagcgt tttattcagc caaatgtgta gctgcctacc ctgctcaata tttagtttta
      181 ccatttaagt attttataat atgtcgaacg gaaataaatt caaatcaaaa caaacggatg
      241 atttacccga agaaaaaccg gtaaaaaagc gcagttcaaa aacaacgcct gccgaacctg
      301 ttcaaactga ggcaatttca aacaattatc aaaaaccgtt acgttggttg gggttgtcgt
      361 gtatgttatt gtctgttttt ttgctcattc cttttttttc atacttgttt acgcatgctg
      421 ccgattttga taaagtaaat caaacttcat ggttatcact tttgggtaat gccgatgtta
      481 tggttgataa ttggttgggt aaattgggtg ccatagtggc tcatgcattt atatacaacg
      541 gttttggcat tgccgcagca gtttttattt tattatcgtt tttactgggt tttaaactgg
      601 tgtttaaagt tgacttgcta ccctggccaa aaacgttgaa atacagtttt tttgcattat
      661 tgtttttaag cataacactg ggttacttaa tgggtaaaca cagtcattta ttatggttag
      721 gtggtgttta tggatatcaa atgagtacat ggttaaatgc aaccattggc gtattaggta
      781 cagggttgtt attagttttt gccgccatat tatttgtggc atctgttttt aaatattcat
      841 accaattgcc ggtatctgca caaaaagcag cacccgaatc ggttgtcgaa aatacacatg
      901 cacctgcagc cggcaatgcc ttgcgcgatg aggtgagcac gccaattgtg atgaaagtta
      961 aagatgaaat tgcactcgaa attgaagatc cggttgagcc tgaaattttt aattctgcta
     1021 tagaagctga tgaaaaggaa gatgatgaag ccgatttgga aatggaagtt ctaatgcctg
     1081 aactaaaacc tgtttcatta gaagtaacgc caaaccagca gcctatcgaa aatgaaaatg
     1141 caattgcagc agtaccaatg gatattgcct tgcccgaaga tgaagccgtt actgctgaaa
     1201 acctattggc caaaatgggt aagtatgacc ctactttgga tttgtctgct tataaatatc
     1261 caaccattga tttgttggaa gattatcaaa gccgaaaaac gcgtcatacg cctgaggaga
     1321 ttaaagccga actcgaagcc aataaaaaca aaattgttgt aaccttagga cattacaata
     1381 ttgaaataga aaaaattaag gccaccattg gcccaacggt aacccttttt gaaattgttc
     1441 ccaaagcagg tattcgaatc tcgaaaatta aaaatctgga agatgatatt gcattaaatt
     1501 taatggccta cggtatc
//
LOCUS       DAOMAA010000600         1516 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_96681_length_1516_cov_3.358658, whole genome shotgun sequence.
ACCESSION   DAOMAA010000600 DAOMAA010000000
VERSION     DAOMAA010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96681_length_1516_cov_3.358658"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /locus_tag="PLO59_08035"
     CDS             complement(<1..1055)
                     /locus_tag="PLO59_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01094.1"
                     /translation="MFKIISGIFIFLCCSVTKATAQCSLNLPDTICPGQTLFLSTGLQ
                     QATFDACIQTPTNTANIVQGIGTNVYEYSVFKDKGKYFAFGQQLGSGYMHRFEFDAQF
                     NFISSQLIAPIPAPSINLHIGSDMIKTDAGKYVAMLPASDAYKVLKFEFDSIDDNNPD
                     VIDFAISNFNAARGCKLYGRYLFVANSGANNITRIEFDSVFQNIIQVIDITGPFSIPW
                     GIDIEFDCATNNYYGFIANDGGLIIRLDFGNSISNPNPTSTIITNTFSETIGIDVERI
                     DNSWYLFSNNFGVLQRYLQFKLDSITAIPTLMIDTVNSALNPFGVTVFADSNITRVFT
                     SANFAHVIFQNQACISSP"
     gene            complement(1126..>1516)
                     /locus_tag="PLO59_08040"
     CDS             complement(1126..>1516)
                     /locus_tag="PLO59_08040"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQV01095.1"
                     /translation="TLPFQLSKGEIIEVYCEHLHSSYILNRNNFVLPISDNLFKVGAT
                     YQWQYLNNQPSIEARMHLAQMLKQITHLPFEITNHWAAIRPTIKDRRPVVRWQQKNVG
                     IINGLGTKGVLLAPLMARQLIDNLPAN"
BASE COUNT          486 a          283 c          266 g          481 t
ORIGIN      
        1 ggagaactga tacatgcctg attttgaaat attacgtgtg caaaatttgc acttgtaaat
       61 acacgtgtaa tattactatc ggcaaatacg gtaaccccaa agggattgag ggcactattt
      121 actgtatcaa tcattaaagt tggtatggca gttatactat ctaatttaaa ctgaagatag
      181 cgttgcaaaa caccaaagtt gttggaaaac aaataccagc tattatcaat tcgttctacg
      241 tcaatgccaa tggtttcgct aaaagtatta gtaatgatgg tgcttgttgg attaggatta
      301 cttatactat tgccaaaatc taacctgatt ataagcccgc catcgtttgc tataaagcca
      361 tagtaattgt tagttgcaca atcaaactct atatcaatac cccagggaat tgaaaaaggc
      421 ccggtaatat caatcacctg aataatattt tggaaaacgc tgtcgaattc aattcttgta
      481 atattgtttg cgcccgaatt ggctacaaat aaatagcggc cgtaaagctt gcatccacgt
      541 gcagcattaa aattagaaat ggcaaaatca attacatcgg gattattgtc gtctatgcta
      601 tcaaactcaa acttcagcac tttataagca tcgcttgccg gcaacatggc cacatatttt
      661 ccggcatcgg tttttatcat atcgctacca atatgcaggt taattgatgg tgccggaatt
      721 ggcgcaatta attgagaact gataaaatta aattgtgcat caaactcaaa cctgtgcata
      781 taacctgaac ccaattgctg accgaatgca aaatacttac ccttgtcttt aaacacacta
      841 tactcatata cattggtacc aatgccttgc acaatattgg ctgtatttgt tggtgtttga
      901 atacatgcat caaacgtggc ttgctgtaaa cctgtactta aaaacaaggt ttgccccggg
      961 caaatagtat caggtaaatt taaactgcat tgcgctgtgg ctttagttac actacaacat
     1021 aaaaaaataa atatgcctga tataatttta aacatgctat ctgattaatg tgctaaatgt
     1081 atgttttgcc attcaaataa ataagccttt atcgtaaaat taaagttagt ttgcaggtaa
     1141 gttatcaatt agctgccttg ccattaatgg tgccaataaa actcctttag tgcctaagcc
     1201 attaattatg cccacatttt tttgttgcca tcttacaact gggcgcctgt ctttaatagt
     1261 aggtctgatg gccgcccaat gattggtaat ttcgaacggc aaatgggtta tttgtttaag
     1321 catttgcgct aagtgcatgc gtgcttctat tgatggttga ttatttaaat actgccattg
     1381 ataagtagcg cccactttaa acaaattatc acttatgggc aacacaaaat tatttcgatt
     1441 taaaatataa ctgctgtgta agtgctcgca atacacttct ataatttcgc cttttgataa
     1501 ctgaaaaggc aaagta
//
LOCUS       DAOMAA010000601         1516 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_96695_length_1516_cov_3.045175, whole genome shotgun sequence.
ACCESSION   DAOMAA010000601 DAOMAA010000000
VERSION     DAOMAA010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96695_length_1516_cov_3.045175"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="PLO59_08045"
     CDS             <1..389
                     /locus_tag="PLO59_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013930243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQV01096.1"
                     /translation="KLLGNIYIELIQALDYEPVKIFANRYWGDLGFIHLCFDVPNMNA
                     LKSEIEKQGYKFTVDSAQTFDMGDSGGRFSYIEDPDGTLIEMVETHKVPVFKKWGIFL
                     NLKNRRKQKPLPNWMVGLLGLNKVKE"
     gene            complement(441..1388)
                     /locus_tag="PLO59_08050"
     CDS             complement(441..1388)
                     /locus_tag="PLO59_08050"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HQV01097.1"
                     /translation="MIYRVTRLIFAVFFKLYYKRITIIGQQNLTNKSTILAVNHPNSL
                     MDALILGVVLKQPIYSLARGDAFKKKWAAKILNAYNMLPIYRQSEGVANLQHNANSFE
                     LCQQLLKQNKTILIFVEGLCENNWQLRPLKKGVARLVQQAWQTDKTAHTQIIPVALTY
                     QHFNGAGKSLIINIGNGITKTSLAMQQTSLAIPLNQIIQNQLQQLMLQYSFLPNTETH
                     KNFINYWFDAEKKYAGIELFNALKKFNNQTATTKSSYNKFAFTIILWPNYLICKYISK
                     RLTTGTVFFDSVLFMLLVFCLPVYLLMVWLFVETLIWIL"
BASE COUNT          501 a          220 c          286 g          509 t
ORIGIN      
        1 gtaaattatt aggcaacatt tatattgagc ttatacaggc cttagattat gagccggtta
       61 aaatttttgc caaccggtat tggggcgatt tgggctttat tcatttgtgt tttgatgtgc
      121 ctaatatgaa tgcgcttaaa tctgagattg aaaaacaagg ttacaagttt acagttgata
      181 gtgcacaaac gtttgatatg ggtgatagtg gtggtaggtt tagctatatt gaagaccctg
      241 acggcacatt aatagaaatg gttgaaacac ataaagtacc ggtatttaaa aagtggggta
      301 ttttcttaaa cttaaaaaac cgcagaaaac aaaaaccatt acccaactgg atggtaggtt
      361 tattgggatt aaataaggta aaagaataac cgttggctaa ggccgaaaag ccttactgct
      421 attttattaa atcaaccatt tcaaaggatc cagattaagg tttcaacaaa aagccaaacc
      481 attaataagt aaaccggcaa gcaaaaaacc aaaagcataa ataataccga atcaaaaaaa
      541 acagttccgg tggttagtcg tttgcttatg tatttgcaaa tcaaataatt tggccataag
      601 ataatggtaa atgcaaattt gttatagctt gatttggttg ttgcagtttg gttgttaaac
      661 ttttttagtg cattaaacaa ttcaataccg gcatattttt tctctgcatc aaaccaatag
      721 ttaataaagt ttttatgcgt ttcagtgttt ggcaaaaatg aatattgtag catgagttgc
      781 tgcaattgat tttgaataat ttgatttaat ggaatagcta aagatgtttg ctgcatagcc
      841 aatgaggttt tggtaattcc attaccaata ttgatgataa gcgatttgcc ggcaccatta
      901 aaatgttggt aagtaagtgc aaccggtatt atttgggtat gagctgtttt atctgtttgc
      961 catgcttgtt gtactaacct tgctacacct ttttttaatg gacgcaattg ccagttgttt
     1021 tcgcataaac cttcaacaaa aattaatatg gttttatttt gttttaaaag ttgttggcaa
     1081 agttcaaacg aatttgcatt gtgttgcaaa ttagccacac cttccgattg cctgtatata
     1141 ggcagcatat tatatgcgtt taaaattttg gcagcccatt tttttttaaa tgcatcgccc
     1201 cttgccaaac tatagatggg ttgttttaat acaacgccaa gtattaaagc atccattaat
     1261 gagttgggat gattgacggc aagaatggtt gatttgttgg taaggttttg ttgcccgata
     1321 atagttatac gcttataata cagcttaaaa aagacagcaa aaattaatcg ggttacccta
     1381 taaatcattt ggtaaataac ttcttttaaa agtatagtac accttacact ttaaaaaatg
     1441 ttgtaataaa ataagttagg tttaattttt taaagcagca tgtaaaatac agctatttca
     1501 ataccaaacg gggtcg
//
LOCUS       DAOMAA010000602         1516 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_96722_length_1516_cov_2.528405, whole genome shotgun sequence.
ACCESSION   DAOMAA010000602 DAOMAA010000000
VERSION     DAOMAA010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96722_length_1516_cov_2.528405"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(256..633)
                     /gene="rplL"
                     /locus_tag="PLO59_08055"
     CDS             complement(256..633)
                     /gene="rplL"
                     /locus_tag="PLO59_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HQV01098.1"
                     /translation="MADLNALADQLVGLTVKEVNELAKILKDNHGIEPAAAAVAVAGP
                     AAGGGAEAPAEKSTFDVILKSAGAAKLGVVKLVKELTGLGLKEAKDLVDGAPKAIKEG
                     ADKATAEDLKKQLEEAGAEVELK"
     gene            complement(764..1288)
                     /gene="rplJ"
                     /locus_tag="PLO59_08060"
     CDS             complement(764..1288)
                     /gene="rplJ"
                     /locus_tag="PLO59_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HQV01099.1"
                     /translation="MTRDEKNKIIDNLVEMLSSYNGFYVTDISTLTVEKTNKLRKLCF
                     DKGVKLNVAKNTLIQKALERMNATEYEPVFSSLKGTSALMLSESANVPAKLIKEFRKA
                     AATDRPVLKCAYIESSVFTGDNQLEALIALKSKNELIADVVALLQSPMRNVLGGLQSG
                     GHKIAGIIKTLESR"
     gene            complement(1310..>1516)
                     /locus_tag="PLO59_08065"
     CDS             complement(1310..>1516)
                     /locus_tag="PLO59_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HQV01100.1"
                     /translation="FKVDKQGHIHTSIGKSSFDASKLSENAAEVLNSIIKLKPSSAKG
                     TYLRSVSLSSTMSPGVKVDPKTVS"
BASE COUNT          426 a          280 c          265 g          545 t
ORIGIN      
        1 tggtgagttg aaattgatgc cactttgtat gttttgtttt ttaaatgcgt tactaaaact
       61 gcatgttgtt agaaatagac ttatttaaaa cacataaaga cccccgccta aggcgagagt
      121 ctgacgtgct ttattaatga ttaactattg aaaatgagta gggcaaggtt agcgaattaa
      181 gtaagttgga aattatagcc aatactttta aggcattgac attagttcga aaacaaatcg
      241 aacagcacca acaaattact ttaattcaac ctcagcacct gcttcttcaa gttgcttctt
      301 caagtcttca gcagtggctt tatcagcgcc ttctttgatt gcttttggag cgccatcaac
      361 taaatcttta gcttctttaa ggcctaaacc ggttaattct ttaaccaact ttacaacacc
      421 taatttagct gcacctgccg actttaaaat tacatcgaaa gttgattttt cggctggagc
      481 ttcagcacca ccacctgcgg ccggacctgc tactgcaact gctgctgctg ctggctcaat
      541 gccgtgattg tcttttaata ttttagctaa ctcgttaact tcttttactg ttagaccaac
      601 taattgatct gctaatgcat ttaaatctgc catgatattt ataattattt aaaggtttaa
      661 aatttatggt tggttattaa gtggaagctc tgtaacctac cggatttaaa ggctgctttg
      721 ttgttatatg cgtaaccatt ttacaacaat tggccttttt gaattatctt gattcgaggg
      781 ttttaataat gcctgcaatt ttatgaccac cactttgtaa tccacccaat acattgcgca
      841 ttggcgattg aagtaatgca actacatcgg ctattaattc gtttttagat ttaagtgcta
      901 ttaaagcctc taattgatta tcgcctgtaa atactgacga ttcaatataa gcgcatttaa
      961 gtactggcct atcagtagct gctgctttgc gaaactcttt tataagtttt gcaggcacat
     1021 tagccgattc gctaagcatt aatgcagaag ttccttttaa tgaactaaat accggttcgt
     1081 attcagttgc attcatacgc tctaatgcct tttgtatgag tgtatttttt gcaacattaa
     1141 gttttacgcc cttatcaaag catagcttac gtaacttatt ggttttttca actgttaatg
     1201 ttgaaatatc ggttacatag aaaccgttat aagatgataa catctcaacc aagttgtcga
     1261 tgattttatt cttttcgtct cttgtcattg tttcaattgt ttaagatgat taactaacag
     1321 tttttggatc taccttaaca ccggggctca tggtacttga taaacttaca ctacgtaagt
     1381 atgtaccttt tgctgaggat ggtttaagtt tgataattga atttaatact tcggcagcat
     1441 tttcagatag tttggatgca tcgaatgatg atttacctat actggtgtga atgtggcctt
     1501 gtttatcaac tttaaa
//
LOCUS       DAOMAA010000603         1515 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_96809_length_1515_cov_3.191096, whole genome shotgun sequence.
ACCESSION   DAOMAA010000603 DAOMAA010000000
VERSION     DAOMAA010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96809_length_1515_cov_3.191096"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1515
                     /locus_tag="PLO59_08070"
     CDS             <1..>1515
                     /locus_tag="PLO59_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01101.1"
                     /translation="VFSFPIQLLLVELKKNQLQLIYWVLIYAIVSGSLFTRYGLHYLF
                     VDPEYLGHVNALSFFIVGISFGTFVMAFNISSYLLNAHRFPFLASLSATFFKFSLNNF
                     ILPTLFFFIYLYRIVSFQYFDELRSLGQVMFNVMGFFAGYTLLVWLVFRFFKVINTDV
                     FKMYEIETADSNSHTKPLTYSFKQGVRNYETTEHPHSWHVEVYLASFTRLRIVRDVSH
                     YPTTMLKAVFKQNHRNAAIFQITLFLIFCTLGLLSDFSLFKIPAAASLLLLFAMFIML
                     AGVVRFWLRGWATLVMLLFFIFINYLTQFPFFNTTSKAYGLNYHIRPADYNLQTLAVN
                     ADSVACVKSLDTTHQILNNWLINQQQAKPKLVLLNISGGGVRSMLFSYKALAVIDSTL
                     QGNLMRHTTLIAGSSGGMIAATYFRQQYVANRFKPLATAQLQEGINAVSKDLLNGIAF
                     KLTVSDLFFNSQRVVDGSYHYVKDRGTAWEKQLTDNLPLLSNKRLIDFKTIEYEA"
BASE COUNT          438 a          257 c          276 g          544 t
ORIGIN      
        1 tgtattttcg tttccaatac agttgttgct ggttgagtta aaaaaaaatc aactgcaatt
       61 aatttattgg gtgttaattt atgccattgt aagtggtagc ttatttaccc gttatgggtt
      121 acattattta tttgttgacc ccgagtattt aggtcatgta aatgcactct cattttttat
      181 tgtaggcatt tcattcggca catttgtaat ggcttttaat atttcaagct acttactcaa
      241 tgcacatcgg ttcccgtttt tagcttcgtt atcagctaca ttttttaaat tcagtcttaa
      301 taattttata ctgccaacat tatttttttt catttatctg tatcgtattg tcagctttca
      361 gtattttgat gagttgcgca gcttaggtca ggtaatgttt aatgtaatgg gattttttgc
      421 cggctataca ttattagtat ggttggtgtt tcgttttttt aaagtaataa ataccgatgt
      481 gtttaaaatg tatgaaatcg aaactgcaga ttcaaacagt cataccaaac cacttacgta
      541 ttcatttaaa caaggtgtac gtaattatga aacaacagaa catccgcaca gttggcatgt
      601 agaagtgtat ctggcatcat ttacaagatt acgaattgta cgcgatgtat cgcattaccc
      661 tacaaccatg cttaaagcag tgtttaagca aaaccaccgc aatgcagcca tttttcaaat
      721 aacattattt ttaattttct gcacattagg cttactaagc gatttttctt tgtttaaaat
      781 acctgctgct gcaagtttac ttttattgtt tgccatgttt attatgttgg ccggtgttgt
      841 taggttttgg ctcagaggtt gggctacatt ggtaatgttg ctttttttta ttttcataaa
      901 ctacctaacg cagtttcctt tttttaatac aaccagtaaa gcctatgggc ttaattatca
      961 catccggcct gcggactaca atttacaaac attggccgtt aatgccgata gcgttgcatg
     1021 cgttaaatca ttagatacta cacatcagat tttaaataac tggttaataa atcagcaaca
     1081 ggccaaacct aaattagtgc tgctcaatat tagtggtggc ggtgttaggt ctatgttgtt
     1141 ttcatataaa gccttagcag ttattgatag caccttgcag ggtaatttaa tgcgccatac
     1201 aactttaata gcaggctcgt caggtggtat gattgccgct acctatttca gacaacaata
     1261 tgtagcaaac aggtttaagc cattagcaac agcacagtta caggaaggga ttaatgccgt
     1321 tagtaaagat ttattaaacg gtattgcatt taaacttacc gtaagcgatt tgttttttaa
     1381 ctcgcaacgt gttgttgatg gcagctatca ttatgtaaaa gacagaggca ctgcctggga
     1441 aaagcaatta accgataacc tgccattact ttcaaataaa aggttgattg attttaaaac
     1501 gattgaatat gaggc
//
LOCUS       DAOMAA010000604         1515 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_96858_length_1515_cov_2.306849, whole genome shotgun sequence.
ACCESSION   DAOMAA010000604 DAOMAA010000000
VERSION     DAOMAA010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96858_length_1515_cov_2.306849"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1515)
                     /locus_tag="PLO59_08075"
     CDS             complement(<1..>1515)
                     /locus_tag="PLO59_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HQV01102.1"
                     /translation="MIEAPEGGLFKSDDGGQSFSRINDERKIRQRAWYYSRIFADTKN
                     QNTVYALNVEFYKSTDSGKNFKAISTPHADHHDLWIDPIDNQRMIVADDGGAQISFDG
                     GDNWSTYYNQPTMQFYRVNTDNHFPYRIYGAQQDNSSIRILSRSNNQSITNNDWENTA
                     GGESGHHAIDPTNDNIVYGGNYGGYLERLNHLTGESRNVSVWPDSPIGWGADSLKYRF
                     QWNFPVFFSPHQKNKLYAAGNQLFTSNNEGQSWTAISPDLTRNNKSTQRASGGIITKD
                     NTTVEYYATIFAAVESAYEPNLLWCGSDDGLIHVSKNGGANWLNVTPANLPKWVLINQ
                     IEVDPFNKGGLYVAATNYKNDDFKPYLLHTTDYGKTWQAITNGINNMHFTRVIKADPK
                     RKGLLYAGTEYGMYISFNDGKNWQTFQQNLPIVPITDLAIKDDDLIVATQGRSFWILD
                     NLTLLHQMEAMDMQQKIKLYKPRNTYLFQHGYTAKPVNAGANPQAGVEIPFYLK"
BASE COUNT          408 a          301 c          299 g          507 t
ORIGIN      
        1 ttttaaggta aaaaggaatt tcgacaccgg cttgtgggtt agcaccggca tttacaggct
       61 tggcggtgta accatgctgg aataagtatg tattacgagg cttgtatagc tttatttttt
      121 gctgcatatc catagcttcc atctgatgta ataaagtaag attgtctaaa atccaaaagc
      181 tacggccttg tgtagcaact attaagtcat catcttttat tgccagatcg gtaataggca
      241 ctatgggtaa attctgctga aatgtttgcc agttttttcc atcgttaaat gatatgtaca
      301 tgccatattc agtacctgca tataacaaac cttttcgttt cggatcggcc tttataacac
      361 gggtaaagtg catgttgttt ataccattgg taatagcttg ccaggtttta ccataatcgg
      421 tagtgtgtaa taaatagggt ttaaaatcat cgtttttata gttggttgct gcaacatata
      481 agcctccttt attaaatgga tcaacttcaa tttgatttat cagaacccat tttggtaaat
      541 tagcaggtgt aacatttagc cagtttgccc caccgttttt acttacatgg ataagcccat
      601 catcgctgcc acaccataat aaatttggtt cataagccga ttcaacggct gcaaaaattg
      661 ttgcataata ttcaacggtt gtattgtctt ttgttattat gccaccgctt gcacgttgtg
      721 ttgatttatt gttgcgcgtt aggtcgggac ttattgcagt ccagctttgg ccttcattgt
      781 tactggtaaa aagttggtta cctgctgcat acaatttatt tttttgatgt ggcgaaaaaa
      841 acacgggaaa gttccattga aagcgatatt tcaaactatc ggctccccag ccgatgggcg
      901 aatcgggcca tacggataca tttctgcttt caccggttaa atgatttaaa cgctctaagt
      961 aacctccata attaccacca tatacaatat tgtcgttggt tgggtcaatg gcatgatgtc
     1021 cgctttcgcc accggctgta ttttcccaat cgttgttagt tatgctttga ttgttactgc
     1081 gacttaaaat acgaatacta ctgttgtctt gctgggcgcc ataaatgcgg tatgggaaat
     1141 gattatcggt attaacccgg taaaactgca tggtaggctg gttataatat gtactccagt
     1201 tgtcgccacc atcaaacgaa atttgtgcgc caccatcatc agctacaatc attcgctggt
     1261 tatcaattgg gtcaatccat aaatcatgat gatcggcatg tggtgtgcta atggctttaa
     1321 aatttttacc gctatcggta cttttataga actcaacatt caatgcataa accgtatttt
     1381 gattttttgt atcggcaaaa atgcgtgaat aataccaggc acgttgcctg attttacgct
     1441 catcattaat gcggctaaaa ctttggccgc catcatcgct tttaaacaaa ccaccttcgg
     1501 gcgcttctat catag
//
LOCUS       DAOMAA010000605         1513 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_97086_length_1513_cov_2.973251, whole genome shotgun sequence.
ACCESSION   DAOMAA010000605 DAOMAA010000000
VERSION     DAOMAA010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97086_length_1513_cov_2.973251"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..965
                     /gene="paaA"
                     /locus_tag="PLO59_08080"
     CDS             45..965
                     /gene="paaA"
                     /locus_tag="PLO59_08080"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004936448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit A"
                     /protein_id="HQV01103.1"
                     /translation="MLEREKAFNDRIARGEVIEPKDWMPERYRKQLIRMMSQHAHSEI
                     VGMLPEGNWITRAPSLSRKASLLAKVQDEAGHGLYIYSATETLGVDRLELTQQFLDGN
                     AKYSSIFNYPTLSWADIGAIGWLVDGAAIVNQTALAKCSYGPYSRAMLRICKEENFHH
                     KQGYQILSIMMQGTAEQKEMAQDAVNRFWWPSLMMFGPSDANSPNSEELMRWKVKLHS
                     NDDLRQRFIDRTVPQGEAIGLTFPDDKLKYSETTRSYEWGQINWDEFKAVVSGNGPCN
                     KQRMQARKTAEENGQWVRAAAEAYAAKHYN"
     gene            1025..1321
                     /gene="paaB"
                     /locus_tag="PLO59_08085"
     CDS             1025..1321
                     /gene="paaB"
                     /locus_tag="PLO59_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018529432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit B"
                     /protein_id="HQV01104.1"
                     /translation="MSTDTKLITWEVFTQKKQGAAHEHAGSVKATDPKMALLNARDVY
                     GRRNEAINMWVVPAFAITASTPEDVGPFFDPANDKLYRNPNFYKLSNTYKPVAD"
     gene            1335..>1513
                     /locus_tag="PLO59_08090"
     CDS             1335..>1513
                     /locus_tag="PLO59_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011046504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaI"
                     /protein_id="HQV01105.1"
                     /translation="MTKNQALYEYALRLGDTSLVLGHRLSEWCGHAPLLEEDIALSNM
                     ALDLIGQSRLMLAYAG"
BASE COUNT          482 a          331 c          326 g          374 t
ORIGIN      
        1 cgctaaaata caactacatt atggaagcta cagttacagc agatatgctt gaaagagaaa
       61 aagcctttaa tgacagaatt gcacgaggcg aagtaattga acccaaagac tggatgccgg
      121 agcgataccg taaacaactg atacgaatga tgagccagca tgcacacagc gaaattgtag
      181 gcatgttgcc cgaaggtaat tggataacac gcgcaccaag cttgtcgcgc aaggcatctt
      241 tattagctaa agtgcaggac gaagccggcc atggccttta catttacagt gcaaccgaaa
      301 ccttaggcgt tgacagatta gagttaacgc aacagttttt agacggcaat gccaaatact
      361 cttccatttt taattaccca actttaagct gggccgatat tggtgccatt ggttggttgg
      421 ttgatggtgc tgcaatagtt aatcaaactg ctttagccaa gtgttcctat ggcccatact
      481 cgcgtgctat gttgcgcatt tgcaaggaag aaaactttca tcataaacaa ggttatcaaa
      541 tacttagcat tatgatgcaa ggtactgccg aacaaaaaga aatggcgcaa gatgctgtta
      601 accgtttttg gtggccttca ttaatgatgt ttggccctag cgatgccaac tcgcccaata
      661 gcgaagaact gatgcgctgg aaagtaaaac tgcacagcaa tgacgacttg cgtcaacgtt
      721 ttattgaccg caccgtgcca caaggcgaag ccattggttt aacctttccg gatgataaat
      781 taaaatacag tgaaaccacc agaagttatg aatggggcca aattaactgg gacgaattta
      841 aagccgtagt tagcggcaat ggcccatgca ataaacaacg tatgcaggca cgtaaaactg
      901 ccgaagaaaa tggacaatgg gtgcgtgcag ctgccgaagc ttatgctgca aaacattaca
      961 attaaaaccc ctgttttagt ttcattttta aaaattgtct tatcaaataa ttaccctaat
     1021 aattatgagt accgatacca aactgataac ctgggaagtg tttacccaaa aaaaacaagg
     1081 tgctgcacat gagcatgccg gcagtgtaaa agcaaccgac cctaaaatgg cattactaaa
     1141 tgcacgcgat gtatatggcc gcagaaacga agccataaac atgtgggtgg taccggcctt
     1201 tgctataaca gcctcaacac ccgaagatgt tggtcctttt ttcgatccgg caaacgacaa
     1261 gttgtatcgc aatcccaatt tttataaatt atccaatacc tacaaacctg tagccgatta
     1321 atgccttatt acctatgaca aaaaatcaag ccctatacga atatgcatta cgcctgggcg
     1381 ataccagttt agtattaggc caccgtttaa gcgagtggtg cggccatgcc ccattactcg
     1441 aagaagacat tgcattatcg aacatggccc tagacctcat tggtcaaagc cggctaatgt
     1501 tagcttatgc agg
//
LOCUS       DAOMAA010000606         1513 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_97096_length_1513_cov_2.796982, whole genome shotgun sequence.
ACCESSION   DAOMAA010000606 DAOMAA010000000
VERSION     DAOMAA010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97096_length_1513_cov_2.796982"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /locus_tag="PLO59_08095"
     CDS             complement(<1..895)
                     /locus_tag="PLO59_08095"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HQV01106.1"
                     /translation="MKLWQKDKAALAEVEIFTVGNDRILDMFLAPFDVLGSLAHIKML
                     ASVHLLTKDELQLLENELKKIYHDIANSNFKLEAGVEDIHSQIELILTQRLGDIGKKI
                     HSARSRNDQVLVDLKLFLRAEIEKIVINTKTLFDLLVSQSEKYKNDLLPGYTHLQLAM
                     PSSFGLWFSAYAESLVDDLLTLEAAYKVVNKNPLGSAAGYGSSFPINRTLTTQLLGFD
                     DLNYNVVYAQMSRGKTERIVASAVANIAATLSKLSMDVCLYLNQNFSFISFPEELTTG
                     SSIMPHKKNPDVFELVRSHCNR"
     gene            complement(914..>1513)
                     /locus_tag="PLO59_08100"
     CDS             complement(914..>1513)
                     /locus_tag="PLO59_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016649883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HQV01107.1"
                     /translation="AIVGEPTEMKMAIAEKGLLVLDCVNYGKAGHAAREEGENAIYKA
                     MKDISWFQNYQFLKKSSMLGPVKMSVTSINTENKAHNIVPADCSFVVDIRVTDAYSHE
                     EILNIIEQHVQAVVKPRSTRLRSSKIEKNHPLIQAGIRLGKKTYGSPTTSDAALINAP
                     VLKCGPGDSARSHIADEFIFLKEINEGLNTYIKLIAQIV"
BASE COUNT          449 a          287 c          253 g          524 t
ORIGIN      
        1 aacgattaca atgcgaacgc acaagttcaa atacatcggg attttttttg tgcggcataa
       61 tgctgctgcc cgtggttaac tcttcgggaa aagaaatgaa tgaaaaattt tgattaagat
      121 ataagcaaac atccatactt agttttgata atgtagcagc aatatttgct actgccgatg
      181 ctacaatgcg ttcggttttt ccacgactca tttgtgcata tactacgtta taattcaaat
      241 catcgaaacc taacaattgg gtagttaatg ttcgattgat tggaaatgat gaaccgtaac
      301 ctgctgctga gcctaatgga tttttattta ctactttata agccgcttcc agtgttaaaa
      361 gatcatcaac aaggctttcg gcgtaggcgc taaaccaaag cccaaacgaa gatggcatag
      421 caagttgcaa atgtgtataa ccgggcaaca ggtcgttttt atatttttcg ctttgtgaaa
      481 ccaataagtc gaataaagtt ttggtattga taacgatttt ctcaatttcg gctctaagaa
      541 ataatttcaa atcaaccaaa acctgatcat tcctgcttct ggcgctatga atttttttac
      601 caatatcacc aagtctttgc gttaatataa gctctatctg tgagtgaata tcctctacgc
      661 cagcttccaa tttaaaattg gaatttgcaa tatcgtgata gatttttttt aattcatttt
      721 ccagtaattg caattcatct ttagtaagca aatgaaccga tgcaagcatt ttaatatgtg
      781 ccagtgaacc taacacgtca aaaggtgcta aaaacatgtc caaaattctg tcgttaccaa
      841 cagtaaatat ttctacttct gctaatgccg ctttatcttt ttgccaaagt ttcatcgtat
      901 gtatatttga tgattaaaca atttgtgcta ttaattttat gtaagtattt aacccttcat
      961 ttatttcttt aagaaaaata aattcatcag caatatggct tcgtgcacta tcgcccgggc
     1021 cacattttaa tacgggtgca tttattaaag cagcatcgga agtagttggt gatccgtatg
     1081 tttttttacc taaccgtatg ccagcctgta ttagtggatg atttttttca atttttgatg
     1141 aacgcaaacg agtactccgg ggttttacta cagcttgaac atgttgttca ataatattta
     1201 aaatttcttc gtggctgtat gcatcggtaa ctcttatatc aaccacaaaa ctacaatctg
     1261 caggtacaat attatgagct ttgttttctg tattgataga tgttacagac atttttaccg
     1321 ggccaagcat ggatgatttt tttaagaact gataattttg aaaccaactt atatctttca
     1381 tggctttata aatagcattc tcaccttctt ccctggcagc atgtcctgct tttccataat
     1441 taacacagtc taacacaagc aatccttttt ctgctatggc cattttcatt tctgttggct
     1501 caccgacgat cgc
//
LOCUS       DAOMAA010000607         1513 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_97108_length_1513_cov_2.594650, whole genome shotgun sequence.
ACCESSION   DAOMAA010000607 DAOMAA010000000
VERSION     DAOMAA010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97108_length_1513_cov_2.594650"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="PLO59_08105"
     CDS             <1..670
                     /locus_tag="PLO59_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HQV01108.1"
                     /translation="SLGLVQAAVLGLTNPTYASNANLQWIPNMDGFPNGRRLEDDVTR
                     IELQAVGGVVLAAVGLWYDDYTAGGPNPVTQDLLDVLTYTTGVETNDKPFKTWFPYMA
                     NPSSGFSACGGALASNSNRMYPGKDEQMKLATPELFMTCTPNPAHTRTSFKYRTTEKS
                     LISLQITDVNGNVVDMPLNNRKTDIGTHDINYDVSKLKSGTYFAMLYSFDQMVQSVKF
                     VVTH"
     gene            746..1453
                     /locus_tag="PLO59_08110"
     CDS             746..1453
                     /locus_tag="PLO59_08110"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HQV01109.1"
                     /translation="MKQFLLFCITGFIATLQAYAQNVTVSGYVLSHDNRGVFGAVISI
                     NNQPKTVTDATGRFAFNLTSENKVSLRVSCLGYTTYTQNVTATGNQTLQIKLTESQID
                     LSEISVSAQSQNTISGIDKVLRPVNNAQELLQLIPGLFVAQHAGGGKAEQIFIRGFDV
                     DHGTDFNITVDGMPVNMVSHAHGQGYADFHFVIPEAISQLQVYKGPYNARFGDFSTAG
                     TGAFFTHQALDKNLVKL"
BASE COUNT          463 a          294 c          300 g          456 t
ORIGIN      
        1 ctccctgggt ttggtacaag cggccgtatt ggggttaacc aatcctactt atgcaagtaa
       61 tgccaattta caatggatac caaacatgga tggtttcccc aatggccgca ggttagaaga
      121 tgatgttacc cgtatcgaat tacaagctgt tggcggtgta gtattggctg ccgtaggttt
      181 gtggtatgat gattatactg caggtggccc aaatccggtt acacaggatt tgttggatgt
      241 acttacttac actaccggtg ttgaaaccaa cgataagcct tttaaaacct ggttccctta
      301 tatggctaat ccatcaagcg gctttagtgc atgtggaggc gcattggcaa gtaactcaaa
      361 cagaatgtac cctggtaaag atgaacaaat gaaattggct acacctgaat tgtttatgac
      421 ttgtacgcct aacccagccc atacacgtac ttcgtttaaa taccgcacaa ctgaaaaaag
      481 tttaatctct ttacagataa ccgatgtaaa tggtaatgta gttgatatgc cattaaacaa
      541 tcgtaaaaca gatatcggta cgcacgatat taattatgat gtaagcaaac ttaaatcagg
      601 aacctatttt gctatgctgt acagttttga tcagatggta caatcagtaa aatttgttgt
      661 tacccattaa ttacttatgc agcctcaatg gtttaacatt ggggctgctt ttttaaacac
      721 atccaacaaa caaacccatg atattatgaa acaattttta cttttttgta taactggctt
      781 tatagcaaca cttcaagcgt atgcccaaaa tgtaaccgta tcaggttatg tattgagcca
      841 tgataaccgg ggggtttttg gggccgttat tagtatcaac aatcaaccta aaacagttac
      901 tgatgcaaca ggccgtttcg cttttaactt aacatccgaa aacaaagttt cattacgtgt
      961 atcttgtttg ggatatacga catatacaca aaatgtaaca gctaccggta atcaaacatt
     1021 gcaaataaaa ttaaccgaat cgcaaataga tttatccgaa atttcagttt cggcacaatc
     1081 gcaaaacacc atttccggta ttgataaagt attgcgtcct gtaaacaatg cgcaggaact
     1141 tttgcaactt atacctggtt tgtttgtagc ccaacatgcc ggtggtggta aggccgaaca
     1201 aatttttata cgtggttttg atgtggacca tggtaccgat tttaatataa ctgtagatgg
     1261 catgcccgtt aatatggttt cgcatgcaca cggacaaggc tatgccgact ttcattttgt
     1321 tatacccgaa gccatatcgc aattgcaagt ttataaaggc ccttacaatg cacgttttgg
     1381 cgattttagt acagcaggca caggtgcatt ttttacacat caggcattag ataaaaattt
     1441 ggtaaagctc taagccggta tgtttgatac ctaccgcgca ttaggtatgt ttaaactgat
     1501 tgataaagag caa
//
LOCUS       DAOMAA010000608         1511 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_97381_length_1511_cov_2.457418, whole genome shotgun sequence.
ACCESSION   DAOMAA010000608 DAOMAA010000000
VERSION     DAOMAA010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97381_length_1511_cov_2.457418"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="PLO59_08115"
     CDS             <1..238
                     /locus_tag="PLO59_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002697609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQV01110.1"
                     /translation="TCYKLFKREYVQSLNLVEKRFGFEPEVTAKIARIPQVRIYEVAI
                     SYYGRTYAEGKKINWKDGFRAIYCIIKYNYFAKR"
     gene            complement(566..>1511)
                     /locus_tag="PLO59_08120"
     CDS             complement(566..>1511)
                     /locus_tag="PLO59_08120"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HQV01111.1"
                     /translation="ATIGCSTINSCKKEESASKPTVDFIYTGGGCTAPCAVLFENKSK
                     DAATFLWDFGDGTTSTASNPTKTYNAGGTYTVSLTATGEGGSASVSKQILIQNSTQSQ
                     LPTANFTFTGGGCMAPCNVNFTNTSANATTYTWDFGDGTSSTSLSPSKIYSTGGSFSV
                     ILTATNAAGTNQITKIVNIASSPTKAKITKVTITNMPFVNSSGASWDFVNGPDVFFNI
                     TDQASNILSSSLSSKINDVTSAALPLAWTYTTAFEINDFNAGRYIDVWDYDTPDPDDY
                     IGYVGFQMSHYTSGSNPYPTTVTKTQNGITVKLDLIWQ"
BASE COUNT          446 a          276 c          297 g          492 t
ORIGIN      
        1 aacctgctac aagcttttta aacgcgagta tgtacaaagc cttaatttgg ttgaaaaaag
       61 atttggattc gaacccgaag ttaccgctaa aattgcacgc ataccacagg tgcgcattta
      121 cgaagtggcc atttcctact acggacgtac ctatgccgaa ggaaaaaaaa taaattggaa
      181 agacggcttt agggcaattt attgtattat taaatacaac tattttgcta aacgctaagc
      241 cgaaagcttg ttgcaggctt taatgtacct ataactacct tatgcattta agcttatgtt
      301 ggttaactat aaaactaaac gctgctcaac taattggagc agcgttttta gttttatatt
      361 gatacaaaat gctactgttt tacaaacttt tttacggtgt ggtttgttgc tgtggttaag
      421 cttaggtgat acatacccgg cattagcgta gcagtaggta cagtaataac atcataagca
      481 tgtggcaggt attgttgcca ttttcaagtt gattttgttt ttaactccaa acaatcatct
      541 ttaaacaacg agtctaaaca tactattact gccaaattag gtcaagcttt actgttatcc
      601 cattctgtgt tttagtaact gttgttggat aagggttact tccgcttgta taatgactca
      661 tttgaaaacc aacatatcca atatagtcgt cagggtctgg tgtgtcataa tcccaaacat
      721 caatgtatcg tccagcatta aagtcattaa tttcaaatgc agtggtataa gtccaagcta
      781 aaggtaacgc tgccgaagta acatcgttta tttttgacga taagctgcta cttaaaatgt
      841 tactagcttg atctgtaata ttaaaaaaca catccggacc atttacaaaa tcccaactgg
      901 caccactgct gtttacaaag ggcatgttag taattgtaac tttcgtaatt ttagcttttg
      961 ttggcgagga tgcaatattt acgattttag ttatctgatt ggttcctgcg gcattagtag
     1021 ctgtgagtat tacagaaaaa gaaccgcctg ttgaatagat ctttgatggg cttaaagatg
     1081 ttgaagatgt gccatcacca aaatcccacg tataagtagt agcattggct gaagtgttgg
     1141 taaaatttac attgcaagga gccatacacc cccctccggt aaatgtaaag ttagctgtag
     1201 gcagttgtga ttgtgttgag ttttgaatta atatttgctt tgacactgag gcagatcctc
     1261 cttctcctgt tgctgttagt gaaaccgtgt aagtaccacc agcattataa gtttttgttg
     1321 ggttagatgc ggtagaagtt gttccgtcac caaaatccca taagaaagtt gctgcatctt
     1381 tcgatttgtt ttcaaacagc actgcgcagg gagccgtgca accaccacca gtataaataa
     1441 aatccactgt tggttttgat gcgctttctt cttttttaca agagttaatg gttgaacatc
     1501 ctattgtggc t
//
LOCUS       DAOMAA010000609         1506 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_97944_length_1506_cov_3.002068, whole genome shotgun sequence.
ACCESSION   DAOMAA010000609 DAOMAA010000000
VERSION     DAOMAA010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97944_length_1506_cov_3.002068"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1506)
                     /locus_tag="PLO59_08125"
     CDS             complement(<1..>1506)
                     /locus_tag="PLO59_08125"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HQV01112.1"
                     /translation="YYNILNESSEINNSVATVINVVPNQPDIIPVGLGISNYYQCFQS
                     EVVARIFNAGATATGNFSARLNVYYNNVLEFTSLQPVSSIQGRCTETVSFLFSPLNIG
                     NYTFEVISDYDSIINESSEINNNLIVPYTFQQCFPDLKVYSCGSMDVKPTDPISPGTI
                     SLYAEIANEGQLTAIAPFVVNFNVAGINYPVTINSNLPAGDTILVSLTAATPAYGNNT
                     LIVSADYANTVTESFEYNNDASAALCWDFATSNADCIGNPLNEVFQSACVPANIRVAL
                     YNFGLYEASQSEVKFEVSGPGIIGWLSLGTSVIFTDNTCICPYIVAAPGSFSYPQTGT
                     YQIRITADPNGLYTECNETNNQIIFSVNVADIPDLTIYSQYIAPSLLNPQINQSITMN
                     VTYKNIGCSAAPASELFVKVDNMPLDSVTAPALAANAFNTVAIGNSWSSFISGFHVIR
                     AIIDNDNQIVEGGESNNEATRSVYVGHAPNFKATNLVVNEMYPQNGENVTL"
BASE COUNT          453 a          285 c          303 g          465 t
ORIGIN      
        1 ttaaggttac gttttcgcca ttttgaggat acatttcatt tacaaccaga ttcgttgctt
       61 tgaagttggg agcatggccc acataaaccg agcgtgtagc ctcgttattg ctttcgcctc
      121 cctcaactat ctgattgtcg ttatcaataa tggctctaat tacatgaaag cccgaaataa
      181 atgagctcca tgaatttccg atggctacgg tattaaatgc atttgcggca agtgcaggtg
      241 ctgtaacaga gtcaagtggc atgttatcca cttttacaaa taattcagat gcaggtgccg
      301 cactacaacc aatgtttttg taagtaacat tcatggttat actttggttg atttgcggat
      361 ttaataatga tggtgctatg tattgcgaat aaattgtaag gtcaggtata tcggctacgt
      421 taacactaaa aataatttga ttattggttt cattacattc tgtgtataag ccatttgggt
      481 cggcagttat gcgtatttgg tatgtacccg tttgcggata agaaaatgaa cccggggcag
      541 caacaatgta tggacaaata catgtattgt cggtaaatat aactgaggta cctaacgata
      601 gccaaccaat tatgccgggg ccacttactt caaattttac ttcgctttgc gaagcttcat
      661 agaggccaaa attgtacaag gctactctaa tatttgccgg cacgcatgcc gattgaaaca
      721 cttcgtttaa aggattgcca atacaatctg cattggaggt tgcaaaatcc caacaaagag
      781 ctgccgatgc atcgttgtta tattcaaacg actcggttac cgtattggca taatcagcac
      841 ttacaattaa tgtattgttg ccataagcag gtgtggccgc ggtaaggcta accagtatgg
      901 tatcgcctgc aggtaaattg ctgtttatag ttaccggata attaatacca gctacattaa
      961 agttaacaac aaagggagct atggctgtta gttggccttc attagcgatt tcggcatata
     1021 agcttatggt gcctgggcta atgggatctg taggttttac atccatgctt ccgcagctat
     1081 ataccttcaa atcaggaaaa cattgctgaa atgtataagg cacaataagg ttgttattta
     1141 tctccgaact ttcatttatt atgctatcat aatcactaat cacttcaaat gtatagttac
     1201 ctatatttaa cgggctaaaa aggaatgaaa ccgtttcagt gcagcgacct tggatactgc
     1261 ttacaggctg taaactggta aattctaaaa cattgttata gtacacatta agcctggctg
     1321 aaaaatttcc tgtagcagtg gcaccggcat taaatatccg ggcaacaacc tccgactgaa
     1381 aacattggta ataatttgaa atgcctaaac ccacaggtat aatatccggc tgattaggca
     1441 ctacattaat aactgttgca acagaattat tgatttcact tgattcgttt aaaatattgt
     1501 aataat
//
LOCUS       DAOMAA010000610         1504 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_98247_length_1504_cov_2.063492, whole genome shotgun sequence.
ACCESSION   DAOMAA010000610 DAOMAA010000000
VERSION     DAOMAA010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98247_length_1504_cov_2.063492"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..641
                     /locus_tag="PLO59_08130"
     CDS             99..641
                     /locus_tag="PLO59_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020289620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HQV01113.1"
                     /translation="MIFIETGLVLMPFLPGDSLLFTAGLFARLGYLDLGLLMALLFVA
                     AVLGDNTNYWVGRKLGLRAIQIKFKDKLLVKPEYVEKTHAFYEKHGAKTIIMARFVPI
                     VRTFAPFVAGIGTMDYRRFLSFDVLGGVIWIGSLTLAGYFLGEIPWIRKNIELVALAI
                     IAISLLPVIVGVIKNRRATA"
     gene            653..1384
                     /locus_tag="PLO59_08135"
     CDS             653..1384
                     /locus_tag="PLO59_08135"
                     /inference="COORDINATES: protein motif:HMM:NF020090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HQV01114.1"
                     /translation="MPAKLFGLIGRQLSHSYSAQYFNQKFAAQNLDALYKLFEIAEIN
                     QLTDLISRYHNLVGLNVTIPYKQEIIHLLDRVTPQALAVNAVNTIKIIDGKCCGYNTD
                     VIGFEQTLQNIHIANKTALIFGFGGAAQAIAYVLKQHQFQFLFVVRCKTNPHQILFSE
                     LDNSIIKQHTLLINCTPVGMYPITNLLPLPYAALTPTHVCYDLVYNPTITPFLAEAQK
                     HGAIIKNGLEMLHLQAEASWKIWQT"
BASE COUNT          471 a          280 c          262 g          491 t
ORIGIN      
        1 tcgattaaat gttagaactg tttacgcaca ttttacatct tgattttgaa tggttgtttt
       61 taaactacaa ccaaactatt tataccatat tatttctggt gatattcatc gaaaccggtt
      121 tggtattaat gcccttttta ccgggcgatt cgctattatt tactgccggt ttatttgctc
      181 gtttgggcta tttagatttg ggtttgctaa tggccttgtt atttgtagct gctgttttag
      241 gcgataatac caattactgg gttggccgta aactcggctt acgcgccata caaattaaat
      301 ttaaagacaa gcttttggta aagcctgaat atgtagagaa aacacatgct ttttacgaaa
      361 agcatggcgc taaaaccatt ataatggctc ggtttgttcc aatagtacgc acttttgcac
      421 cgtttgttgc aggcattggc accatggact atcgcaggtt tttatcattt gatgtattag
      481 gtggtgttat atggatagga tcacttacac tggccggtta ttttttaggc gaaattccct
      541 ggataagaaa aaatatcgag ttagtagcat tggctataat agccatttca ttgttaccag
      601 taattgttgg tgtaattaaa aaccgcaggg ctactgctta actttttaat aaatgccggc
      661 aaaattattt ggtttaattg gcaggcagct tagtcactcc tactctgccc aatactttaa
      721 ccaaaagttt gctgcacaaa atttagacgc actctataag cttttcgaaa tagccgaaat
      781 aaatcaatta accgatttaa tttcgcgcta ccataattta gttggcttaa atgttaccat
      841 accgtataaa caagaaatta ttcacttatt agacagggta acaccgcaag ccttagctgt
      901 taatgccgta aataccatta aaattattga tggcaaatgt tgtggctaca acaccgatgt
      961 tattgggttt gaacaaacac tgcaaaacat acacatagct aataaaacag cattaatttt
     1021 tggttttggc ggagcagcac aagccattgc atacgtactt aaacaacatc aatttcaatt
     1081 tctatttgtg gtccgatgca aaaccaatcc gcatcaaata cttttttcag aattagataa
     1141 ttcgataatt aagcaacaca cattacttat caattgcaca ccggtaggca tgtacccaat
     1201 aaccaacttg ctgccactac cttatgctgc gcttacgcct acacatgttt gttacgattt
     1261 agtgtataac cccaccatta cacccttttt agctgaagcc caaaaacatg gagccatcat
     1321 aaaaaatggt ttagaaatgt tgcacctgca agcagaagca agttggaaaa tttggcaaac
     1381 ataaagcaca tggcataatg cgatacaata ttgattatat tgaaagtaaa agcatggttg
     1441 tacatagttt atacaaagtt tatcttgcta caatttcata ttataaataa tgattgattc
     1501 catt
//
LOCUS       DAOMAA010000611         1502 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_98424_length_1502_cov_3.433310, whole genome shotgun sequence.
ACCESSION   DAOMAA010000611 DAOMAA010000000
VERSION     DAOMAA010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98424_length_1502_cov_3.433310"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1308
                     /locus_tag="PLO59_08140"
     CDS             <1..1308
                     /locus_tag="PLO59_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ligase PGM1-related protein"
                     /protein_id="HQV01115.1"
                     /translation="PRTHVIYVTSAPIEESIIDYYLTLLPGITGYHARQRLTLFSCYD
                     TSDKSLTQKIVDRPRLVQRIREKIRDVEKAHLTCFNVTEYEKQLALALNIPIYGCDPR
                     LLYLGTKTGSRLIFKKLGIPVPTGFENLKNDHEIATALAKLKIDNPKLEKAVIKMNDG
                     FSGEGNAIYYYGDLKATDDKLPNVILQTLPLHTKVVAEHISFIAFMSQFNEMGGIVEE
                     FVLGDVKESPSVQCRINPLGQTEIISTHDQLLGGEGGQVFIGATFPANKAYNADIAKM
                     GNSIAEELRSLGVLGRFGIDFISVKQPDNTWQHFAIEINLRKGGTTHPFMMLQFLTNG
                     EFNWKDGIYTMSNGQQRCYFASDNVVSEKYIGITPHDLIEIAMNNHLLYDGSKQTGVM
                     FHMIGALSQHGKIGVVCIGKTNDEAREFYERTIQLLDKESTYH"
BASE COUNT          486 a          271 c          306 g          439 t
ORIGIN      
        1 ccacgcaccc atgtaatata cgtaaccagt gcacccatcg aagaaagtat tattgattat
       61 tacctcacac ttttgcccgg cattaccggt tatcatgcaa ggcaacgatt aaccttgttt
      121 agttgttatg atacatctga taaatcgtta acgcaaaaaa ttgttgacag acccagactg
      181 gtgcaacgca tacgcgaaaa aatacgcgat gttgaaaaag cacatttaac atgctttaat
      241 gttaccgagt atgaaaagca gttggctttg gctttaaaca ttccgattta cggatgcgac
      301 ccgcggctat tgtatttagg tactaaaacc ggtagccgtt taatatttaa aaagttgggc
      361 ataccggtgc ctaccggttt cgaaaattta aaaaacgacc acgaaattgc caccgcttta
      421 gccaaactta aaattgataa tcccaaatta gaaaaagccg ttataaaaat gaatgatggc
      481 ttttcgggcg aaggaaatgc catttactat tatggcgatt taaaagctac tgacgataaa
      541 ttacccaatg tgattttgca aacattgcca ttacatacca aagtggttgc agaacatata
      601 agctttattg cttttatgag tcagtttaac gaaatgggtg gcattgttga ggagtttgtt
      661 ttgggtgatg ttaaagaatc gccatcggta caatgtcgca taaacccttt aggccaaacc
      721 gaaattattt caacccacga tcagttgttg ggtggcgaag gtggtcaggt gtttattggg
      781 gctacatttc cggcaaataa agcatacaat gccgatattg ctaaaatggg caatagcatt
      841 gccgaagagt tacgcagttt aggtgtgctt ggtcgttttg gtattgattt tatttcggtt
      901 aaacaacctg ataatacctg gcaacatttt gcaatagaaa ttaatttgcg caagggcggt
      961 actacacatc cttttatgat gttgcaattt ttaaccaatg gcgagtttaa ttggaaagac
     1021 ggtatataca ccatgagtaa tggccagcag cgttgttact ttgcttctga taatgtagta
     1081 agcgaaaaat acattggcat aacaccacac gatttaattg aaattgccat gaataaccac
     1141 ctactgtacg atggcagcaa acaaaccggt gtaatgtttc acatgattgg cgctttatcg
     1201 caacatggca aaattggtgt ggtttgcatt ggcaaaacca atgacgaagc gcgtgaattt
     1261 tacgaaagaa ccattcagtt attggataaa gaaagtactt atcattaatt cataccggag
     1321 tggtagcacc tgcttagttt atgtatgtaa aattaaactt gccccaggtt gatttaaagt
     1381 tcaaacaaac gggcgatata catttggtat atgatgtaat acgtaaaaag tatatccaaa
     1441 taacacctga agagtgggta aggcaacata taattcacta ttgtatccag ttaaaatatc
     1501 cc
//
LOCUS       DAOMAA010000612         1502 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_98446_length_1502_cov_2.886662, whole genome shotgun sequence.
ACCESSION   DAOMAA010000612 DAOMAA010000000
VERSION     DAOMAA010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98446_length_1502_cov_2.886662"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="PLO59_08145"
     CDS             complement(<1..861)
                     /locus_tag="PLO59_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01116.1"
                     /translation="MANKTTFAPDFKTWHKCRKSKQKMNTKTSNNKNLYATWVDSQKK
                     MIDSLTESTQKFTKDETVNNTIEKGSALYKNWLDNQLSFMNEQTDKMQKNPASALQQE
                     EITETAKNWMKNQMELTKSFMDFSMNTMKEYMQSLNKSMPVLNGNGDKMTKMFNEHVD
                     TFKKWSNTLNKQVEEMLQNFDNQTAKNAMAGMFNQTDVYNKFVELWTPVIKALQTNNF
                     NTDSFNQLVNPAQFKTFMDKMLGWNKEAMENYAGAFNLNGNPFNEYMTQLSNMNKNWM
                     NLMQPSNNPAN"
     gene            1066..1476
                     /locus_tag="PLO59_08150"
     CDS             1066..1476
                     /locus_tag="PLO59_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014797682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HQV01117.1"
                     /translation="MIQVGDTFKHTFSYTQDDVNTYARVSGDTNPLHIDEAAGKASMF
                     GRCIIHGYLGGSVFTKLFGTTVYADGNVYMKQLMQFMRPMFTGVNYEAVITVAEIFPE
                     KNRVLYKTEVFNVDTKELTITGEALLMNKKQYVW"
BASE COUNT          419 a          240 c          255 g          588 t
ORIGIN      
        1 atttgccgga ttgtttgatg gctgcatcaa attcatccag tttttattca tgttgcttaa
       61 ctgtgtcatg tactcattaa acggattgcc atttaagttg aatgcacctg catagttttc
      121 catagcttct ttattccatc ccaacatttt atccataaat gttttgaatt gagccggatt
      181 aactaattgg ttaaacgaat cggtattgaa gttgtttgtt tgtaatgctt tgataacggg
      241 tgtccacaac tcaacaaatt tattgtaaac atcagtttga ttaaacatgc ctgccattgc
      301 atttttagca gtttgattat caaaattttg aagcatttct tcaacttgtt tgtttaaagt
      361 attgctccat tttttaaatg tatcaacatg ctcattaaac atcttagtca ttttatcgcc
      421 attgccattt aaaaccggca ttgatttgtt aagcgattgc atgtattcct tcatggtatt
      481 catcgaaaaa tccataaaac ttttagttag ctccatttga tttttcatcc agtttttagc
      541 tgtttcggta atttcttctt gttgcaaagc tgatgcagga tttttttgca ttttatcagt
      601 ttgctcattc ataaacgaaa gctggttatc aagccagttt ttgtacaatg ccgaaccttt
      661 ttcaatcgta ttatttacag tttcgtcttt tgtaaatttc tgtgttgatt cagtaaggct
      721 gtcaatcatc tttttttgtg agtcaaccca ggtggcgtac aaatttttgt tgttgcttgt
      781 tttagtgttc attttttgtt ttgatttgcg gcatttatgc catgttttaa aatcgggcgc
      841 aaaagtagtt ttatttgcca cacctaaaac aatttattgt taattgattt agcagtgctt
      901 tttaattggt ttgaaatagc ttattttatt ggtttttgaa aggtttttgt cgttgttttt
      961 gattgttaaa atattgtaaa acatgtcctt ttgtttagaa acttaaatta ttttcttcct
     1021 aacctcgcat ttttattaat ccaattaaac ttaaaaccca aaattatgat tcaagtaggc
     1081 gatactttta agcacacctt tagttacacc caagatgatg taaacaccta tgcacgtgtt
     1141 tcgggcgata ccaatccttt acatattgac gaagctgcag gtaaagcatc tatgtttggt
     1201 cgttgtatta tacacggcta tttaggtggt agcgttttta caaaattgtt tggcactacg
     1261 gtatatgccg atggcaatgt gtacatgaaa cagcttatgc aatttatgcg acctatgttt
     1321 accggtgtta actatgaagc tgttattacg gtagccgaaa tttttcctga aaaaaacaga
     1381 gtgctttata aaaccgaagt ttttaatgtt gacactaaag agttaaccat aacaggtgag
     1441 gcattattga tgaataaaaa acaatacgtt tggtagttaa aaagcagttt gaaaaactgc
     1501 tt
//
LOCUS       DAOMAA010000613         1501 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_98595_length_1501_cov_2.849931, whole genome shotgun sequence.
ACCESSION   DAOMAA010000613 DAOMAA010000000
VERSION     DAOMAA010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98595_length_1501_cov_2.849931"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..710)
                     /locus_tag="PLO59_08155"
     CDS             complement(21..710)
                     /locus_tag="PLO59_08155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HQV01118.1"
                     /translation="MIQKYKTIFFDLDHTLWDFEANSTATLLELISKYALQQWAAPAS
                     FIEVYQQINQQMWQLYHQQQIDKEALRLQRFVKTLAYFNINDNKLAAQLSDEYLAICP
                     HKGQLFAHAHEVLEYLQSKYQLLIITNGFREVQNIKIDAAQLRSYFHHCIVSEDVGHL
                     KPNAPIFEYAMQLANSSPKQCLMIGDNYEADIIGAADVGIDTVLFNAPQPNKLTATYQ
                     VQNLKQLLQFL"
     gene            complement(757..1164)
                     /locus_tag="PLO59_08160"
     CDS             complement(757..1164)
                     /locus_tag="PLO59_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01119.1"
                     /translation="MKFVVTPSFAALYQSSNQLAYWQVCLLCIIGGMGGVLVFSYFTV
                     PIFNFIDWVKKKIFQHKLKPRKVFTKRNRLIVKIWRGYGLFGIAALTPVLISIPIGTI
                     VACHLERRRTKVFLYLFISIVTWSLLLNSVYYL"
     gene            complement(1244..>1501)
                     /locus_tag="PLO59_08165"
     CDS             complement(1244..>1501)
                     /locus_tag="PLO59_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HQV01120.1"
                     /translation="ILCDPMLATGQSLVLTYKALLLSGKPKHTHIVAGIASVQGVNHV
                     KAHLPENVTIWCGDVDEELTSKSYIVPGLGDAGDLAYGNKR"
BASE COUNT          479 a          282 c          271 g          469 t
ORIGIN      
        1 atctgttggc ggttttttta ttataaaaac tgaagcagtt gttttaaatt ttgtacctga
       61 taggttgccg ttaatttatt gggttggggt gcattaaaca aaaccgtatc aatacccaca
      121 tctgccgcac ctataatatc ggcttcatag ttatcgccaa tcatcaaaca ttgtttggga
      181 gatgaattgg caagctgcat ggcatactca aatataggtg cattgggttt taaatgtcct
      241 acatcttcgc ttactataca atgatgaaaa taactacgta attgcgctgc atcaatttta
      301 atgttttgca cttctctaaa tccgttagta ataattaaga gctgatactt tgactgcaga
      361 tattctaaaa cctcgtgggc atgtgcaaac aattgtccct tatgcgggca aatagccagg
      421 tattcatcac tcaattgggc tgcaagctta ttatcattta tattaaagta cgctaatgtt
      481 tttacaaagc gctgcaagcg caaagcctcc ttatcaattt gttgctgatg gtacaactgc
      541 cacatctgct gattgatttg ctgatatact tcaatgaaag atgcaggtgc tgcccattgt
      601 tgcaatgcat acttgctaat aagttccaac aaagttgcag ttgagttggc ttcaaagtcc
      661 cagagtgtat gatctaaatc aaaaaaaatg gttttatatt tttggatcat tactgaagaa
      721 tgtaatagct ataagcttct taaaacattt gcaaaattat aagtagtaca cactgttaag
      781 cagtagcgac catgttacta ttgaaataaa gagatataaa aaaaccttgg tccgcctgcg
      841 ctctaaatgg caagctacaa tagtgccaat aggtattgaa ataagaacgg gtgtaagagc
      901 agcaatacca aacaaaccat agccacgcca tatttttaca atgagtctgt tgcgttttgt
      961 aaaaactttt cggggtttta atttatgttg aaatattttt tttttaaccc aatcaataaa
     1021 attaaaaatg ggtacggtga agtaagaaaa aaccaaaaca ccacccatgc cacctatgat
     1081 gcatagcaaa caaacctgcc aataggctaa ttgattgctt gattgataga gtgctgcaaa
     1141 actgggtgtt actacaaact tcacactgct taaaagcaca attaaaattg atttgtgtaa
     1201 aacctccaat ttacaatact gtttagacct ttaatttcaa tagttatcgt ttgttgccat
     1261 aagctaaatc accggcatcg cctaaaccgg gcactatata tgatttagaa gttaactcct
     1321 catcaacatc gccacaccaa attgttacgt tttcgggcag atgtgcttta acatgattaa
     1381 cgccttgtac acttgctatg cctgcaacaa tgtgtgtgtg tttgggcttg ccacttaaca
     1441 aaagtgcttt ataagttagc accaacgatt gcccggtggc taacatggga tcgcataaaa
     1501 t
//
LOCUS       DAOMAA010000614         1501 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_98618_length_1501_cov_2.549793, whole genome shotgun sequence.
ACCESSION   DAOMAA010000614 DAOMAA010000000
VERSION     DAOMAA010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98618_length_1501_cov_2.549793"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="PLO59_08170"
     CDS             <1..382
                     /locus_tag="PLO59_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008624975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HQV01121.1"
                     /translation="TILLTPENKTVILANGAVSNGTIVNYSKHGNMRVDLTLAVTTDA
                     NLTHVRKVALDTMAANDKVLKTPEASVNVLKIGDGMITLAIRPFCTPENYWDVFFSVQ
                     EDLKTAFENNNIAGPTPTRIIINK"
     gene            complement(430..>1501)
                     /locus_tag="PLO59_08175"
     CDS             complement(430..>1501)
                     /locus_tag="PLO59_08175"
                     /inference="COORDINATES: protein motif:HMM:NF024774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenine deaminase C-terminal domain-containing
                     protein"
                     /protein_id="HQV01122.1"
                     /translation="PTINTDHECFTLEEALDKLQHGMHILIREGSAAKNFNELHSLIA
                     SHTGKVMFCMDDAHPDDLIKGHINLIVARAIALGYNLFDVLQCACINPHLHYKTKSGV
                     LQTGHPADFIIVDDLINFKVHATFINGTKVVSNCVCNLPDRKHLHINRFNIPAIDLQS
                     LQLKTEATQQVNVRVIEALEGQLITNETEAYLMPQNGLLQSDVKQDVLKMVVVNRYQT
                     ASVAHAFIKNFNLTVGAIASTVAHDSHNIICVGVTDDAIQQAINAVINHKGALSVYDG
                     KHVSSLPLPIGGLMSDANATDTAYIYSKLNSIIKTMGCTLHAPFMTLSFMALLVIPDL
                     KLSDKGLFSGKSFTYTPLIIND"
BASE COUNT          479 a          285 c          256 g          481 t
ORIGIN      
        1 taccatactg cttacaccag aaaacaaaac cgttatactg gctaatggtg ccgtatcaaa
       61 cggtaccata gttaattaca gtaaacacgg taatatgcgc gttgatttaa ctttagccgt
      121 tactactgat gctaacctaa cccatgtacg caaagttgca ttagatacca tggctgcaaa
      181 tgacaaagtt ttaaaaacac ctgaagccag tgtaaatgtt ttaaaaattg gtgacggcat
      241 gattactttg gctatccgac ctttttgtac gcctgaaaat tattgggacg ttttttttag
      301 tgtgcaggaa gatcttaaaa ccgcatttga aaacaataat attgcaggcc ctacacctac
      361 ccgcattatt ataaacaaat agaaaagagc tgcaaaatgc agctctttta atttcaatcc
      421 attttaattt tagtcgttaa ttattaatgg agtgtatgta aacgacttgc ccgaaaacaa
      481 acctttgtca cttaatttta aatcgggaat tactaaaagc gccataaatg ataacgtcat
      541 aaaaggtgca tgtagcgtac accccattgt ttttataatg ctattaagtt ttgaataaat
      601 atatgcagta tctgtagcat tagcatcact cattaaaccg ccaataggta atggcaaact
      661 gctaacatgc ttgccatcat aaaccgataa agcgccttta tgattaatta ctgcattaat
      721 agcttgctgt atggcatcat cggtaacacc tacacaaatt atattatgcg aatcgtgcgc
      781 aaccgttgat gcaattgcac ctacagttag gttaaagttt ttaataaatg catgtgccac
      841 cgatgcagtt tggtatcggt taacaactac catttttaat acatcctgct taacatcgct
      901 ttgtaacaaa ccattttgtg gcattaagta agcttcggtt tcattagtta taagttggcc
      961 ttctaatgct tcaataacac gtacattaac ttgttgtgtt gcctctgttt ttagttgcaa
     1021 actttgtaag tcaattgcag ggatattaaa cctgttaata tgcaggtgct ttctatccgg
     1081 taaattacat acgcaattcg aaactacctt tgtgccatta ataaaagttg catgtacttt
     1141 aaaattaatt aagtcatcaa cgataataaa atcggctgga tgcccggttt gcagcacacc
     1201 tgatttggtt ttataatgca ggtgtggatt tatacatgcg cattgtaaaa catcaaataa
     1261 attatagccc aatgcaatag cacgtgctac tatcaggttt atatgtcctt ttattaaatc
     1321 atcaggatgt gcatcgtcca tacaaaacat aaccttacct gtatgcgaag ctataagtga
     1381 atgcaattca ttaaaatttt tggctgcact tccctcgcga attaaaatat gcataccatg
     1441 ctgcaactta tcaagtgctt cttctaaagt aaaacattcg tggtcggtat tgatggtggg
     1501 c
//
LOCUS       DAOMAA010000615         1498 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_98958_length_1498_cov_3.288981, whole genome shotgun sequence.
ACCESSION   DAOMAA010000615 DAOMAA010000000
VERSION     DAOMAA010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98958_length_1498_cov_3.288981"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="PLO59_08180"
     CDS             <1..322
                     /locus_tag="PLO59_08180"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HQV01123.1"
                     /translation="AIMLGKKVAACVMYYGMPESNAVKLKNICPVLGIFGNDDTRITP
                     KIVDRFQTIMQQVNQTLVVYRYDAKADFANPYNTNHHVEATNQAYNQTISFIKKMLLQ
                     MPAH"
     gene            334..>1498
                     /locus_tag="PLO59_08185"
     CDS             334..>1498
                     /locus_tag="PLO59_08185"
                     /inference="COORDINATES: protein motif:HMM:NF017197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HQV01124.1"
                     /translation="MLKRTKKIALLLLAVLVAAIVFAFAFISPIAKYLIHKYDKEFIN
                     REVKIDGLWINILSGSLALKDVTLFELDGKTPFVKFTKLESQISIPKLIGGAYDISYV
                     KLTDAQVWLTFKNGKFNYEDMVAKFSNTDTNPNPVNEAPLKYWVKNIVIQNGAVTYNN
                     PDLQYLIEAIKINISTPGIAYNQNNLNSKIDLAFKSGGAISTTFNTNLSNSDYSMQAA
                     ITNFDLHVWYPYLKDLMNTKDLLGTINTTFNLKGNFNTPEQIALSGKLDLNHFAILDP
                     LNIPLVTCTKLDVGVDTLNVSKGLYNLSQIDFTQPYVLFEMYDNGNNFYRLLSDTTIS
                     AAQAIEEASADGNMFTLMFGYVADIAKNFTITNYALQQFKINQGHVIYHDYTLP"
BASE COUNT          518 a          292 c          255 g          433 t
ORIGIN      
        1 ggcaattatg ctcggtaaaa aagtggctgc ttgtgttatg tattatggca tgcccgaaag
       61 caatgctgta aagcttaaaa acatttgccc ggtattgggt atatttggta atgacgatac
      121 aaggattact cctaaaattg ttgaccggtt tcaaacgatt atgcaacaag taaaccaaac
      181 attggtggtt tatcgttatg atgcaaaggc cgattttgca aatccataca acaccaacca
      241 tcacgtagaa gccactaacc aggcctacaa tcaaaccata tcttttatta aaaaaatgtt
      301 gctgcaaatg ccggcacatt aatcacacac cccatgctta aacgcaccaa aaaaattgcg
      361 ctactgcttt tagctgtatt agttgccgct attgtatttg catttgcttt tatatcgcct
      421 attgctaagt atctgataca taagtatgat aaagaattta taaaccgcga agtaaaaatt
      481 gatggcttat ggattaatat tttatcagga agcttggcat taaaagatgt tacgcttttt
      541 gagcttgatg gtaaaacacc gtttgtaaaa tttaccaaac tcgaatcgca aatctcaatt
      601 ccaaaattga taggtggcgc ctatgatata agctatgtta aactaaccga tgcacaagtt
      661 tggttaacat ttaaaaacgg caagtttaat tatgaagata tggttgccaa gttcagcaac
      721 accgatacca accctaatcc cgttaacgaa gctccgttaa agtattgggt aaaaaacatt
      781 gtcatacaaa acggggccgt tacatacaat aatccggatc tgcaatattt aatcgaagcc
      841 ataaaaatta atatcagcac accgggcatt gcatacaacc agaataacct gaacagtaaa
      901 attgaccttg cgtttaaaag tggaggagcc ataagcacta catttaatac caacttatct
      961 aacagcgatt acagtatgca ggcagccata accaatttcg atttgcatgt gtggtatccc
     1021 tatttaaaag atttaatgaa taccaaagat ttattaggca ccatcaatac aacatttaat
     1081 ctgaaaggca acttcaacac gcccgaacaa attgcccttt cgggtaaatt agatttaaat
     1141 cattttgcca ttttagatcc tttaaacatt ccgttagtta catgcactaa attagatgta
     1201 ggtgttgata cactaaacgt aagcaagggc ctttacaatt taagccaaat agattttaca
     1261 caaccctatg tactatttga aatgtacgac aatggcaata atttttaccg cctgctgtca
     1321 gatacaacca taagtgctgc acaggcaatt gaagaagcca gtgccgatgg aaatatgttt
     1381 accctcatgt ttggatacgt tgccgatatt gccaaaaact ttacaataac aaactatgct
     1441 ttgcaacagt ttaaaattaa tcaagggcat gtgatatatc atgactatac actaccgc
//
LOCUS       DAOMAA010000616         1492 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_99777_length_1492_cov_2.531663, whole genome shotgun sequence.
ACCESSION   DAOMAA010000616 DAOMAA010000000
VERSION     DAOMAA010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99777_length_1492_cov_2.531663"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..1166)
                     /gene="obgE"
                     /locus_tag="PLO59_08190"
     CDS             complement(150..1166)
                     /gene="obgE"
                     /locus_tag="PLO59_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015809183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HQV01125.1"
                     /translation="MENNFIDYVKINCRSGKGGSGSMHLHRDKLTAKGGPDGGNGGRG
                     GHIIIRGNKQLWTLLHLKYRKHVLGHDGQSGQSANKTGAEGKDEILEVPLGTIARDFE
                     TGQQLFEINNDGQTEILMHGGRGGKGNAFFKSSTMQTPRFAQPGEPGQEGWKILELKL
                     MADVGLVGFPNAGKSTLLSVVSAAKPEIANYPFTTLAPNLGMVYYRHNRSFIMADIPG
                     IIEGAHLGKGLGLRFLRHIERNSVLLFLLPADSNNLKDEYKILKKELRLHNPDLMQKR
                     RLIAITKCDMLDNELLKELKKEVPKDKNKDGSKVDVLFISSVAQQGIEALKDAVWNAI
                     NADV"
     gene            complement(1156..>1492)
                     /locus_tag="PLO59_08195"
     CDS             complement(1156..>1492)
                     /locus_tag="PLO59_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HQV01126.1"
                     /translation="ITFMFCSFDKVPNILRLYGSGQTILPSHKTYKDYSQYFEMPIGV
                     RQFIYSKIHRVQTSCGYGVPLFDFVADRNTLNQWAVNKGNTLHQYQQEKNLISIDGLP
                     TALNLNHGK"
BASE COUNT          422 a          304 c          273 g          493 t
ORIGIN      
        1 tattgtttaa taaacagtac aaacagcaat gcatataatg gtatccaaat aaaacgattg
       61 cttgccaaaa tcattatcca atctaatatg ggctgattat ggctattaat ccacaataaa
      121 agtttagtat ttatttgttg ccacatattt taaacatcgg cattaatagc attccaaacg
      181 gcatctttta atgcttcaat gccttgttgg gcaacgcttg aaataaataa aacatcaacc
      241 ttactgccat ccttattttt atctttaggt acttcctttt ttaattcttt caaaagttca
      301 ttatcaagca tatcgcattt agtaatagca ataagtctgc gcttttgcat taaatcggga
      361 ttatgcaaac gcagttcttt tttcagaatt ttatattcat cctttaaatt attgctatcg
      421 gcaggcagca gaaataataa aacactgtta cgttcgatgt gccgtaaaaa ccgtaagcct
      481 aaacctttac ccaaatgggc accttcgata atgcccggta tgtcggccat aataaacgat
      541 cggttatggc gataatatac catgcctaag tttggcgcca gtgtggtaaa tgggtagttt
      601 gcaatttcgg gttttgctgc cgaaaccaca ctgagcaagg tacttttgcc ggcattcgga
      661 aaacctacca agcccacatc ggccataagt ttaagttcta aaattttcca gccttcttgg
      721 cccggttcgc ccggttgtgc aaagcgtggt gtttgcatgg ttgatgattt aaaaaaagca
      781 ttgcctttgc cgccacgtcc gccatgcatt aatatttcgg tttgtccatc gttattaatt
      841 tcgaacaatt gctggccggt ttcaaaatcg cgagcaatgg tacctaaggg tacttctaaa
      901 atttcgtctt tgccttcggc accggtttta ttagccgatt gcccgctttg cccatcatgg
      961 cccaaaacat gttttcggta ttttaaatgc agtaaagtcc ataactgttt gttgccgcgt
     1021 ataattatat gtccgccacg tccgccattg ccaccatcgg ggccgccttt tgctgtaagt
     1081 ttatcgcggt gtaagtgcat agaaccactg cctcctttgc cactgcggca atttattttt
     1141 acataatcaa taaaattatt ttccatggtt taggtttaaa gcagtgggta aaccatcaat
     1201 gctaatcagg tttttttctt gttgatattg atgtaaggta ttgcctttgt ttacggccca
     1261 ttggttaagt gtatttctat cggccacaaa atcaaataaa ggcacaccat agccacatga
     1321 tgtttgtacc ctgtgaattt ttgaataaat aaattgacgc acacctatgg gcatttcgaa
     1381 atattgcgaa tagtctttat atgttttatg tgatggtaaa atagtttgtc cgctgccgta
     1441 cagtcttaaa atattgggca ctttatcgaa actgcaaaac ataaaagtaa ta
//
LOCUS       DAOMAA010000617         1492 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_99804_length_1492_cov_2.242867, whole genome shotgun sequence.
ACCESSION   DAOMAA010000617 DAOMAA010000000
VERSION     DAOMAA010000617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99804_length_1492_cov_2.242867"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PLO59_08200"
     CDS             complement(<1..242)
                     /locus_tag="PLO59_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01127.1"
                     /translation="MRKLILIILLSTNQLKAQYIPTWNNDIACIIYSRCASCHNANGI
                     APFSLIDYNDAVNYSPGIQIAVSNKIMPPWPPDPTY"
     gene            357..713
                     /locus_tag="PLO59_08205"
     CDS             357..713
                     /locus_tag="PLO59_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQV01128.1"
                     /translation="MSKYDEIAKQWFNAFNTKNTELLLSLYDDNAQHFSPKLLIARPE
                     TKGLVTGKAAMRDWWQQAFDTIKGLNYELLTLVTNEQHIFFEYIRHADDVADLRVGEL
                     LEIRNGLIVSSRVFHG"
     gene            755..>1492
                     /locus_tag="PLO59_08210"
     CDS             755..>1492
                     /locus_tag="PLO59_08210"
                     /inference="COORDINATES: protein motif:HMM:NF035938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EboA domain-containing protein"
                     /protein_id="HQV01129.1"
                     /translation="MQKKAIEFILTVIVSKVDTDTYAEIKYACDEVAQNADKLKTCYV
                     KALRRYNKQKITYTQKQLSEANSICNGWLPPLTLVDAVRTLLLLHTKFSDTQMQLSNL
                     QSMVDCGDNNEQIVIYQNLALLPHADTLALFCAQGLRSNMADIFEAIANNNPYPAKYL
                     ADDAFNQMVLKCVFIGKPLYKIVGLHQRLNPNLSRMIIDYAAERWAASRSLSPEVWQL
                     TPIIENAHKDLYLKLQRSNIESEQKAAS"
BASE COUNT          492 a          243 c          293 g          464 t
ORIGIN      
        1 ctataggtag gatcgggcgg ccagggtggc attattttat tgcttactgc tatttgaata
       61 cccggtgaat aattaacagc atcgttataa tctattaatg aaaaaggtgc aataccgttg
      121 gcattgtggc aactggcgca acgactgtaa ataatacaag caatatcatt attccaggtg
      181 ggtatgtact gggcttttaa ttggttagtg cttaatagta tgattaatat cagtttacgc
      241 attttgagta atatttaagt tccaaataaa aataattaat agaattttgc cttgataaac
      301 ttagacaatt aattcagtat gtgcagggtt tatttttcac actaaatttt aggcaaatga
      361 gtaagtatga cgaaatagca aaacaatggt ttaatgcatt taatactaaa aacacggaat
      421 tgttgctgag cctgtacgat gataatgccc aacattttag tcccaaatta ttaattgccc
      481 ggcccgaaac caaaggattg gtaaccggta aagcagctat gcgagattgg tggcaacaag
      541 cttttgatac gattaaaggt ttaaattatg agttgctgac tttggttact aatgagcagc
      601 acattttttt tgagtacata agacatgctg atgatgttgc tgatttgcgc gttggcgagt
      661 tgctcgaaat tcgcaatgga ttaattgttt catcgcgtgt atttcatggg taaactacca
      721 ttcattaaaa tctgaatttt gtaattatac cgttatgcaa aaaaaggcca ttgaatttat
      781 actaacagtt attgtatcaa aagttgatac cgatacctat gccgaaatta aatacgcatg
      841 tgatgaagta gcacaaaatg ctgacaagct taaaacctgt tatgtaaaag cattgcgcag
      901 gtataacaaa caaaaaatta catatacaca aaagcaatta agtgaagcaa attcaatttg
      961 caatggttgg ttaccaccgt taactttagt tgatgcagtg cgtaccctgt tactgttgca
     1021 tacaaaattt agcgatacac aaatgcaatt aagcaatttg caaagtatgg ttgattgtgg
     1081 cgataacaat gagcaaatag ttatctatca aaatttagcg ctgttgccac atgccgatac
     1141 attggcattg ttttgtgcac aaggcttacg aagtaatatg gccgatatat ttgaagcaat
     1201 tgccaataat aatccttacc ctgcaaaata tttagccgat gatgctttta atcagatggt
     1261 tttaaaatgt gtttttatag gtaagccttt gtataaaatt gtggggctgc atcaacgttt
     1321 aaatccaaac ttatcacgta tgataattga ctatgcagcc gaacggtggg cggcttctcg
     1381 aagcctttcg cccgaagttt ggcagttaac gcccatcata gaaaatgccc ataaagattt
     1441 gtatttaaaa cttcagcgca gcaacattga atcagaacaa aaggctgcat cg
//
LOCUS       DAOMAA010000618         1488 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_100296_length_1488_cov_2.920447, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000618 DAOMAA010000000
VERSION     DAOMAA010000618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100296_length_1488_cov_2.920447"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="PLO59_08215"
     CDS             complement(<1..351)
                     /locus_tag="PLO59_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQV01130.1"
                     /translation="MAQGILSGKKGIIFGALDENSIAWQVAMQAHAEGAQIVLTNAPV
                     AMRMGAINNLATAINAPVIPADVANNDDMHNLIAKSMEHFGGGVDFILHSVGMSLNVR
                     KGKTYTDLDYDFMHK"
     gene            830..>1488
                     /locus_tag="PLO59_08220"
     CDS             830..>1488
                     /locus_tag="PLO59_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009333235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HQV01131.1"
                     /translation="MAAEKITQQNGKLNVPANPIIPFIEGDGIGPDIWAASVRVLDSA
                     VSKAYNGNRKIEWKEVLAGEKAFNKTGNWLPDETLDIIREYLIAIKGPLTTPVGGGIR
                     SLNVALRQQLDLYTCLRPVRWFSGVPSPVKHPEKTDMVIFRENTEDIYAGIEYMAGTD
                     DVKKVIAFLTETLKVKNIRFPEDSSIGIKPVSISGSERLIRAAIQYAITHGKPSVTLV
                     H"
BASE COUNT          507 a          274 c          259 g          448 t
ORIGIN      
        1 tttatgcata aaatcataat ctaaatcagt gtatgttttg cccttacgta catttaacga
       61 cataccaact gaatgcaaaa taaaatcaac accaccacca aaatgctcca tactttttgc
      121 aattaaattg tgcatatcgt cattattagc cacatcggca ggtataaccg gagcattaat
      181 tgcagtagct aagttgttta ttgcgcccat acgcatggca accggtgcat tggtaagtac
      241 tatttgcgca ccttcggcat gtgcttgcat ggctacctgc caggctattg agttttcgtc
      301 taaggcgcca aaaatgatac ctttttttcc ggataaaata ccttgtgcca taatgtaatt
      361 ttttagagta agttgttatt aatataagtt gttatggttt actgtatggc aacaaaccta
      421 caccatataa aaacaaaaca aaatattttt tactgccctg tttacaatct gattctaaaa
      481 agaaacgtca actgttattc atttttaaag gactttacaa aaaaaatgtc tgcataaact
      541 attatccaat ttgatgtttc aaaaataaga tccggtttta atcatcaatt caactcaaat
      601 taccttagtt tgaaacaata catgttagta gcaacagtaa caggtatagc agcatttcaa
      661 taaaataaac atcaataacc aacatgtcta taatggtgta accaaaaaca gtggccaaca
      721 agtatattaa aaggcataat gaattcagat gctatctatc ctttttaggc ttgcaatttt
      781 ttctacattc gccaccctta aaaattagtt tacgataaaa aaaataaaaa tggcagcaga
      841 aaaaataaca caacaaaacg gaaaattaaa tgtacccgct aacccaatta ttccatttat
      901 tgaaggtgat ggtattggac ccgatatttg ggctgcatct gtgcgcgtac tggatagtgc
      961 cgttagtaaa gcttataacg gtaaccgtaa aattgaatgg aaagaagttt tagcaggtga
     1021 aaaagcattt aacaaaaccg gaaattggtt gcctgatgaa actttagaca tcatacgcga
     1081 atacctaatt gctattaaag gaccacttac tacgcctgtt ggtggtggta tccgttcact
     1141 taatgtggca ttacgtcagc aattagattt atatacatgt ttgcgtccgg tacgttggtt
     1201 tagcggtgta ccctcgcctg ttaaacatcc cgaaaaaaca gatatggtaa tttttcgcga
     1261 aaacactgaa gatatttatg caggcattga atatatggcc ggcactgatg atgtaaaaaa
     1321 agtaattgca tttttaaccg aaaccttgaa ggttaaaaac atccgctttc ctgaggatag
     1381 cagcattggt ataaaaccgg tttcgataag tggtagcgag cgtttaatac gtgccgctat
     1441 tcaatatgca ataacgcatg gcaaaccatc ggttactttg gtgcacaa
//
LOCUS       DAOMAA010000619         1486 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_100557_length_1486_cov_3.408805, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000619 DAOMAA010000000
VERSION     DAOMAA010000619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100557_length_1486_cov_3.408805"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="PLO59_08225"
     CDS             complement(<1..639)
                     /locus_tag="PLO59_08225"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HQV01132.1"
                     /translation="MKTSITYYLIIFQIIFGYQISKAQSSNALAFDGSNDLVTIPNAS
                     ALISGSNAISLACWVYPTNPTPNTSSYDGIVGIRNNTDADFYILHFTANTVECRFRNS
                     NGNNFDLIGTGIVLNAWQHLAFTYDGSMLRYYRNGVLTDSLPANGDITLNNESLFIGG
                     TPYQGNQFLLEGRVDEVTFWDKAINGTDLNCLLNGSADTSDANLLLYYKFNQG"
     gene            771..>1486
                     /locus_tag="PLO59_08230"
     CDS             771..>1486
                     /locus_tag="PLO59_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01133.1"
                     /translation="MAVIAVFKYQTKQPVNKAFYYWQQTYNPSLSENSLMDTLAVNKL
                     YIKYFDVVWDANLLRAQPVATVHFNKPCNKDYVPVVYITNEVFKNLSKKSVDSLYINI
                     TKLILAINVNYNPTEIQFDCDWTDKTTEKYFKFLHLFKSDKRFSNVNLSATIRLHQIK
                     YKSRTGVPPVDRGMLMFYNMGKLKQIETTNSIFNNYDAGKYVSYLDNYPLPLDYALPV
                     FGWSIYFRNGKSQGILNDTYA"
BASE COUNT          515 a          236 c          245 g          490 t
ORIGIN      
        1 tccctgatta aacttgtaat acaaaagtaa gtttgcatca cttgtatcgg cacttccatt
       61 taacaggcag tttaaatcgg taccgttaat tgctttatcc caaaaggtta cttcgtcaac
      121 acgtccttct aaaagaaatt gattaccctg gtatggtgta ccaccaataa aaagcgactc
      181 attatttaaa gtaatatcac catttgcagg taacgaatca gtaagtacgc cattacgata
      241 atatcttaac atgctaccat catacgtaaa tgccaaatgt tgccacgcat ttaaaacgat
      301 gcctgtgcca atcaaatcaa aattgtttcc gttactgttt ctgaaacgac attcaaccgt
      361 attggccgta aaatgaagaa tataaaaatc ggcatcggta ttatttctga tgcccacaat
      421 accgtcataa ctgctggtat tgggtgttgg gttagtagga tacacccagc aagccaatga
      481 aatagcatta ctacccgata ttaaagcgga tgcattgggt atggtaacca aatcattact
      541 accatcaaac gctaaggcat tactgctttg tgctttcgaa atttgatatc caaaaatgat
      601 ttgaaaaatt attaaataat aagtaattga tgttttcata gtttcattta ttgaacaaat
      661 tggttgattt ttagtttatt cgaaattaaa aataatttat ttacaaggtg gcaaagttat
      721 caaacaaaac aatttgggtt ttaacaagct ttgttatatt tcttgtaatt gtggctgtaa
      781 tagctgtttt taagtatcaa acaaaacaac ctgttaacaa ggcattttat tattggcaac
      841 aaacttataa cccttcgtta agcgaaaata gtctaatgga tacattagcg gttaacaaac
      901 tatacattaa atattttgat gtggtgtggg atgcaaattt attaagggcg cagcctgttg
      961 caacagtaca ttttaataaa ccgtgtaata aagattatgt gcctgttgtt tatattacca
     1021 atgaagtttt taaaaattta tcaaagaaaa gcgttgacag tttatatata aatattacaa
     1081 aattaatttt agcaattaat gtgaattata atccaacaga aatacaattt gattgtgact
     1141 ggacagataa aacaacagaa aagtatttta aatttttaca tctttttaaa agtgacaaaa
     1201 ggtttagtaa tgtaaacctt agtgctacta ttcgtttaca tcaaataaaa tacaaatcgc
     1261 gtactggtgt accaccggtt gataggggca tgctcatgtt ttacaacatg ggtaagctta
     1321 aacaaattga aacaactaac tctatattta acaactatga tgcaggcaag tatgtatctt
     1381 atcttgataa ttatccgctg cctttagatt atgctttgcc ggttttcggg tggagtattt
     1441 attttagaaa tggcaagtcg cagggtatat taaatgatac ctatgc
//
LOCUS       DAOMAA010000620         1486 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_100564_length_1486_cov_3.159329, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000620 DAOMAA010000000
VERSION     DAOMAA010000620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100564_length_1486_cov_3.159329"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      complement(29..145)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            251..>1486
                     /locus_tag="PLO59_08235"
     CDS             251..>1486
                     /locus_tag="PLO59_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01134.1"
                     /translation="MKALLTHIAFVLIMPCLMAQNIKDEIINYSYAQLPLQPLNREIK
                     NFNSYIEAPYEAENKKQLAAYEQSKVDAKVAYDKAMITYTQQLKAADVQYAKDMEAWE
                     KKSTGNKIVEKTLLGANTKPVKQSVYPPAAPYVAPPVLRSSYDYTTLANTYINLGGYA
                     RDTANAVQIIVTLYGLDYTLPRQQSVVKDMVRSANGQTQSYKATYYYTEFSYRHPMAV
                     KVYDPAGKELLIATPQELNNYKIYKTAESENPPTINQELLIKTYEEKVLQDNLTFINK
                     MVNDKFGYALVNRKTNLYYVKSKDGLYADLLLAFNEADAGLKTLVADAATAKPKITTA
                     IQAWEKALTESEPTNKKARIDNDVTIAICFNLLEGYFALGDVFHAENIIKKMNTLDLS
                     GDERKTKVGYELLFADFKKR"
BASE COUNT          551 a          268 c          271 g          396 t
ORIGIN      
        1 aagttaataa accataaaaa tcattttcga cttatctgat attgcacaaa tgcaatactg
       61 gaattagcac ctttggtaat actgtgtatg taccgtggtt gctatggttt cattgggcct
      121 gtccctccac cattctggat aagtatttat aaagaactgc cgcaaatata atggcttaaa
      181 taagaaaatc aaattgtctg atttttaaat ttaatacttc tacattgggc accattaaac
      241 aatttattga atgaaagcgt tattaactca cattgccttt gtgctgatta tgccatgttt
      301 aatggctcaa aatattaaag atgaaattat caactactca tatgcgcaac tgcccttgca
      361 gccattaaac agagaaataa aaaatttcaa ttcatatatt gaagcacctt acgaagcaga
      421 aaataaaaaa caattggctg catacgaaca aagtaaagtg gatgcaaagg ttgcttatga
      481 taaagccatg attacctata cgcagcaatt aaaagcagcc gatgtacagt atgcaaaaga
      541 tatggaagca tgggagaaaa aaagtacagg aaataaaatt gtagaaaaaa cattgttggg
      601 cgccaacaca aaaccggtta agcaaagtgt atatccgccc gcggctcctt atgtagcgcc
      661 accggtttta cgcagcagtt atgactacac aaccttagct aatacataca ttaacctggg
      721 aggctatgca cgcgatacgg caaatgctgt acaaattata gtaacgctgt atggtttaga
      781 ttacacatta ccacgtcagc aatcggttgt aaaagacatg gttcgcagcg ccaatggcca
      841 aacacaatca tacaaagcaa cttattacta cacagagttt agttaccggc atccgatggc
      901 cgttaaggtg tatgaccctg ccggaaaaga actattaatt gctacaccgc aagagctgaa
      961 taattataaa atttataaaa cagcagaaag cgaaaatcca ccaaccatta accaggagct
     1021 gctaataaaa acgtatgaag aaaaagtatt gcaagacaat ttaaccttta tcaataaaat
     1081 ggttaacgat aagtttggct atgctttagt aaacagaaaa accaacttgt actatgtaaa
     1141 aagcaaagat ggtttgtatg ccgatttatt gcttgctttt aacgaggctg atgcaggatt
     1201 aaaaacactt gttgctgatg cggctacagc caaacccaaa attaccactg ctattcaagc
     1261 ttgggaaaaa gcactaacag aatcagaacc cacaaataaa aaagcaagaa tagacaatga
     1321 cgttaccatt gcaatatgtt ttaatttatt ggaaggttat tttgcattgg gagatgtgtt
     1381 ccatgcagaa aacatcatta aaaaaatgaa tacgttagac ctgtcgggcg atgaaagaaa
     1441 aaccaaggtt ggctatgaat tactttttgc tgactttaaa aaaaga
//
LOCUS       DAOMAA010000621         1486 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_100573_length_1486_cov_3.045423, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000621 DAOMAA010000000
VERSION     DAOMAA010000621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100573_length_1486_cov_3.045423"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="PLO59_08240"
     CDS             complement(<1..220)
                     /locus_tag="PLO59_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018920056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HQV01135.1"
                     /translation="MQAPTLLPTSQVTAKKLVVGPCSAESWEQLLQTAYQIAANYPHA
                     IFRAGVWKPRTVPGLFEGAGTEALPWLAD"
     gene            427..>1486
                     /locus_tag="PLO59_08245"
     CDS             427..>1486
                     /locus_tag="PLO59_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01136.1"
                     /translation="MKKPLLYVTIIIYCVAGMQKKGSAQNNLPIPKHFDEAVINNFRT
                     HKGVPGSAYFTNYAHYKMDVKLDINQASVFANGIIRYFNASADTLHKLSFKLIQNVHQ
                     PVAQRQVTVNNDYLTNGINITQTTVNGMPHTLHADPGNITNGTLILDRPLLPHDSLTI
                     TLKWHYQLNRVTDDIREGVVDSNSFFVAYWYPQIAVYNERRWDKTPHTEQAEFFNEFA
                     NYDVSITVPQNYLVVATGTLQNANEVYTESIAGRIEEAATNDDINLCVLKFDIDNKQV
                     TQPKENTWHFLADSVTDFAWGTSNHYLLDAASIMVDSITKRRVLIQVAYDTSAKNYSD
                     VCFWSQQALSYLSFQMPAV"
BASE COUNT          468 a          286 c          293 g          439 t
ORIGIN      
        1 catcggccag ccagggcaat gcttcggtac ccgccccttc aaataaaccg ggtacagtgc
       61 gcggtttcca cacacctgct ctaaaaatgg catgcgggta gtttgctgca atttgatatg
      121 cggtttgaag caattgttcc caactttcgg cactgcatgg ccccactaca agtttcttgg
      181 ctgtaacttg tgaagtaggt aaaagtgttg gtgcctgcat tttatttaga tgcgaattat
      241 attttgacaa tatcaaagta tgcttgtaaa cgcacaaagg ttaaaaatgg tttcgacatt
      301 gaaactattt ttattgatga agcaaaaaac cgtttttcat ttgattaaca atgatcggtt
      361 ataagtttag tttttaaatt gaatatgtca tgcaaggcaa aaattatatt tggccaaacc
      421 attgttatga aaaaaccatt gctttatgta actatcatta tttattgtgt agccggcatg
      481 caaaaaaagg gtagtgcaca aaataatttg cccataccaa agcattttga tgaggctgta
      541 atcaacaatt tcagaaccca taagggtgtg ccgggtagtg catatttcac caattatgcg
      601 cattataaaa tggatgtaaa actggatatt aatcaagcat cggtttttgc aaatggtatt
      661 atcaggtatt ttaatgcatc ggccgatacg ttacataaac ttagctttaa acttatacaa
      721 aatgtgcatc agcctgttgc gcaacgacaa gtaaccgtta acaacgatta tctgaccaat
      781 ggcataaata ttacgcaaac cactgttaac ggcatgcccc ataccttaca tgccgacccg
      841 ggtaacatca ccaatggtac tttgatatta gatagacctt tattgccaca tgatagcctt
      901 actataactt taaagtggca ttatcagctt aaccgtgtaa cagacgatat acgtgagggt
      961 gttgttgata gcaattcgtt ttttgtagct tattggtatc cgcaaattgc tgtttacaac
     1021 gagcgcagat gggataaaac gcctcatacc gaacaagccg agttttttaa tgaatttgct
     1081 aattacgatg taagcataac cgtaccacaa aactacctgg tggtggcaac aggcacctta
     1141 caaaatgcca acgaagttta tacggaaagt atagcaggac gtattgaaga agcggccacc
     1201 aacgatgata ttaatttatg tgttttgaaa tttgatatag ataataaaca ggttacccaa
     1261 cccaaagaaa acacatggca ttttttagcc gactcggtta ccgattttgc atggggtacc
     1321 agtaatcatt acttattaga tgctgccagc atcatggttg acagcattac caaacgaagg
     1381 gtacttatac aagtagctta cgataccagt gcaaaaaatt acagcgatgt ttgcttttgg
     1441 tcgcagcaag ccttatcata ccttagtttt cagatgccgg cagtag
//
LOCUS       DAOMAA010000622         1486 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_100582_length_1486_cov_2.883997, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000622 DAOMAA010000000
VERSION     DAOMAA010000622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100582_length_1486_cov_2.883997"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="PLO59_08250"
     CDS             complement(<1..300)
                     /locus_tag="PLO59_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018126555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HQV01137.1"
                     /translation="MNLKQILSGLQSVDQVGVEERIARLGKRSIKNQSKVDALKLALQ
                     MCDLTTLEARDTEGKVIQLCNKAMHPHESLNVPSVAAVCVYPNMVAVAKKSFAGSK"
     gene            complement(340..852)
                     /locus_tag="PLO59_08255"
     CDS             complement(340..852)
                     /locus_tag="PLO59_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01138.1"
                     /translation="MHISYTETQYFGNWIRIILILLLFPVGMLSAVKLSNAGNTSHLL
                     AAIITFAISVALLLLFWKAHLSTSIDNYGISYQFKPFHLKQKTINWDDVKLVELMKYK
                     PIQMYGGWGLRYSFKYGWAYNVSGNYGLKIYAANKKPVLIGTNQLEKMQETIMKLNQD
                     CKLKYKLSLN"
     gene            complement(863..1021)
                     /locus_tag="PLO59_08260"
     CDS             complement(863..1021)
                     /locus_tag="PLO59_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA binding domain-containing
                     protein"
                     /protein_id="HQV01139.1"
                     /translation="MADKKAIVIRISPEISKQLEKWAVDEFRSLNGQIEYLLQKALLE
                     QGRLRKKN"
     gene            complement(1034..>1486)
                     /locus_tag="PLO59_08265"
     CDS             complement(1034..>1486)
                     /locus_tag="PLO59_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020605592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HQV01140.1"
                     /translation="YVKFVNIQSEAAVRHLAGTCPYDNFEDADAVTTLRGGDDVVNDM
                     LRMQLDERLNKAGIRVTEARISHLAYAPEIAGAMLQRQQATAIVAARQQIVEGAVGMV
                     EMALEQLAKKQVVTLDEERKAAMVSNLLVVLCGDRNVSPVVNTGTLHN"
BASE COUNT          435 a          326 c          227 g          498 t
ORIGIN      
        1 cttactaccc gcaaaacttt ttttagcaac agccaccatg tttggataaa cacaaacagc
       61 agcaacagat ggtacattta aactttcatg cggatgcatt gccttattac aaagctgaat
      121 tactttacct tcagtatcac gagcttccag tgtagttagg tcgcacattt gtaaggctaa
      181 ttttaatgca tcaactttcg attgattttt aatactccgt ttccccagcc gtgctatccg
      241 ttcttctacg ccaacctgat ctacagattg taaccctgac aagatttgtt ttaagttcat
      301 attcggctaa ttttttcaaa cataagtaaa tccaaacttc taatttaaag aaagcttata
      361 tttcaattta caatcttgat ttagcttcat aattgtttct tgcatctttt ctaattgatt
      421 ggtgccaata agtacgggct ttttattggc tgcgtaaatt tttaacccat aattacctga
      481 aacattgtat gcccaaccgt atttaaaact ataccttaat ccccatccac catacatttg
      541 gattggttta tatttcatta actctactaa tttaacatca tcccaattaa tagttttttg
      601 tttcagatga aagggcttaa actgataact gattccatag ttgtcaattg aggtgcttaa
      661 atgcgctttc caaaacagca acaacagtgc aacagatatt gcaaacgtaa ttatggctgc
      721 aagcaaatgc gatgtgttac ctgcatttga caattttaca gccgaaagca tgcctactgg
      781 aaatagtaat agaattaaaa taatgcgaat ccagtttccg aagtattgtg tttcagtata
      841 agatatatgc atgcccattc ttttaatttt ttttacgcaa acgtccttgt tctaacaatg
      901 ccttttgaag caggtattca atctgtccat ttaaactcct gaactcatca acagcccact
      961 tttcaagctg cttactaatt tcagggctaa tcctaattac aattgctttt ttatcggcca
     1021 caataaaaaa cgtttaatta tgtaaggtgc cggtatttac aacagggctg acatttctat
     1081 cgccacaaag cacaactaat aaattactaa ccatagccgc tttacgttct tcatctaaag
     1141 taactacttg ttttttagcc aattgttcca aggccatttc aaccatgcct acggcacctt
     1201 ctacaatctg ctgacgggca gctacaatag ctgttgcttg ctgcctttgt aacatggcac
     1261 cggcaatttc gggtgcatag gctaaatggc tgatgcgtgc ttctgttact cttatgcccg
     1321 ctttatttaa tcgctcgtct aattgcatgc gcaacatatc attaaccaca tcatcgccac
     1381 cacgtagcgt agttacagca tcggcatctt caaaattatc ataagggcaa gtacctgcta
     1441 aatgccttac tgctgcttcg ctttgtatgt ttacaaactt aacgta
//
LOCUS       DAOMAA010000623         1486 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_100609_length_1486_cov_2.334731, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000623 DAOMAA010000000
VERSION     DAOMAA010000623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100609_length_1486_cov_2.334731"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="PLO59_08270"
     CDS             <1..342
                     /locus_tag="PLO59_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018945068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HQV01141.1"
                     /translation="DGGGDGGGSGGGSGDGKGDGKGSGVKLNLAGRKWLRPPRIDDKS
                     QETGKVVVAIIVDKQGKVISAIPGDVGSTTNSAYLYKLAKEAALSTPFNASPDGKETQ
                     KGTISFNFVLE"
     gene            401..>1486
                     /locus_tag="PLO59_08275"
     CDS             401..>1486
                     /locus_tag="PLO59_08275"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HQV01142.1"
                     /translation="MQASAYKTTIDYLFKQLPMFHRIGAAAYKANLNNTHALMQLLNQ
                     PQQGFKSIHVAGTNGKGSTSHMLASILQHAGYKVGLYTSPHLNDFRERIKINGKPISK
                     KYVIDFVETYKADFEKIQPSFFEWTVGLCFDYFRNQQIDIAVIETGLGGRLDSTNVIT
                     PLLSVITNIGYDHMALLGNTLPEIASEKAGIIKSKTPVVIGKYQNSTAPVFKLKAKSE
                     NAPINFASKILHADLLSLRNGLQFVNITAGKNLRYKKLALDLTGTYQLENVTTVLAAV
                     AKLQSNFNINEIAIRNGLKTVVKTTGLMGRWQTIQTKPLIICDTGHNVDGITYVLKNM
                     ESIKYKKLHIVIGIVADKDVSHILKRLP"
BASE COUNT          504 a          280 c          289 g          413 t
ORIGIN      
        1 gatggcggtg gagatggtgg cggcagcggt ggtggcagtg gcgatggtaa aggagatggc
       61 aaaggctcgg gtgtaaaatt aaatttagca ggtcgtaaat ggttacgtcc accacgtatt
      121 gatgataaat cgcaagaaac gggcaaggtt gtagtagcca ttattgttga taaacaaggt
      181 aaagttatta gcgccatacc gggcgatgta ggctcaacta ctaacagcgc ctacctttac
      241 aaattggcta aagaagctgc tttaagcaca ccgtttaatg ccagccccga tggtaaagaa
      301 actcagaagg gaaccatttc atttaacttt gtgttagagt agcttttcga aaacaaactt
      361 tgatttattg ctataatccg ctattcattt taaatgtatc atgcaggcat cagcatataa
      421 aacaaccatt gattatttat ttaaacagtt gcctatgttt catcgcattg gtgcagcggc
      481 ttacaaggct aatttaaata acacccatgc gctgatgcaa ctattaaatc agccccaaca
      541 aggttttaaa agcatacacg tagcaggcac taatggcaaa ggaagtacat cgcacatgct
      601 ggcttctata ttacaacatg ccggttacaa ggttgggcta tatacatcgc cacatttaaa
      661 tgatttcaga gagcgcatta aaattaatgg caaacccatt tcaaaaaaat atgtaattga
      721 ctttgttgaa acatacaaag ctgatttcga aaaaattcag ccatcattct tcgaatggac
      781 tgtaggctta tgttttgact atttcagaaa tcaacaaatt gatattgctg ttattgaaac
      841 cggtttaggt ggtcgtttag attctacaaa tgttattaca cccttgctta gcgtaattac
      901 caatattggt tatgaccaca tggctttgct tggcaatacg ctacctgaaa ttgcttctga
      961 aaaagccggg ataattaaaa gcaaaactcc ggtagtaatt ggcaaatatc aaaacagtac
     1021 agcacctgta ttcaaactga aagcaaaatc agaaaatgcg cccataaact ttgcatctaa
     1081 aatactgcat gccgatcttt tatccttgcg caatggctta cagtttgtaa acataacagc
     1141 cggcaaaaat ttacgctata aaaaacttgc cttagattta accggcactt atcagttaga
     1201 aaatgtaaca actgtattag ctgctgttgc aaagctgcaa agtaatttta atattaatga
     1261 aatagccata agaaacgggt taaaaacggt ggtaaaaact actggactaa tgggacgctg
     1321 gcaaaccatc caaaccaaac cgttaataat ttgcgacacc ggccataatg ttgacggcat
     1381 tacctatgtg cttaaaaaca tggaatcaat taaatacaaa aaactgcata tagttattgg
     1441 cattgtagcc gacaaagatg tatcgcacat tttaaaacgc ttacct
//
LOCUS       DAOMAA010000624         1484 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_100871_length_1484_cov_2.400280, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000624 DAOMAA010000000
VERSION     DAOMAA010000624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100871_length_1484_cov_2.400280"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..945)
                     /locus_tag="PLO59_08280"
     CDS             complement(<1..945)
                     /locus_tag="PLO59_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HQV01143.1"
                     /translation="MKKLLIIFFGFYSLIAQATHNRAGEITYRQISLFYYEATIVTYT
                     KTSSAIDRPVLELHWGDNTFDTVSRVNQASVGTDITKNTYIGYHNYAAAGIYKLHCED
                     MNRNGGVINIPNSINTSFYIETLLQINPFLGFNNSPVLLNPPIDQGCSNQTFMHNAGA
                     FDPDGDSLSYELFICRGNGGLPCPGYSFPTATVSFSLDSITGDLRWQNPNSIACGEWN
                     VAFLIKEWRNGVNIGYIERDMQITIYCNCPNNTPQLNLVTTDTCVEAGTFITLAATAT
                     DPDNNPISLTASGGPFLITPDSAYLTTTPPPPTTKILNW"
     gene            complement(1153..>1484)
                     /locus_tag="PLO59_08285"
     CDS             complement(1153..>1484)
                     /locus_tag="PLO59_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dehypoxanthine futalosine cyclase"
                     /protein_id="HQV01144.1"
                     /translation="GIRNAVTADEYVRMIALSRIMLPNIKNIQASWLTVGKATAQICL
                     HAGANDFGSIMIEENVVSAAGAPHRFTAQGIQDAIAEAGFEPQLRNQQYQFRAMPQNM
                     EQQVINY"
BASE COUNT          438 a          286 c          304 g          456 t
ORIGIN      
        1 ccagtttaaa attttagtgg taggtggtgg tggtgtggtg gtaagatatg ccgagtcggg
       61 cgtaattaaa aaaggcccac ccgaagctgt tagcgatatg ggattgttat caggatcggt
      121 agcggtagct gctaaggtta taaaagtacc ggcttctaca caagtatcgg ttgttactaa
      181 attaagttgc ggtgtattgt tagggcaatt acaataaatg gtaatctgca tatcgcgttc
      241 tatgtagcca atatttacac cattgcgcca ttcttttatt aaaaaggcaa cattccattc
      301 gccacaggca atgctgttgg ggttttgcca acgcaaatca ccggttatgc tatctaagct
      361 aaacgatacc gtagccgttg gaaatgaata tcccggacag ggcaaaccac cattgccgcg
      421 gcatataaat aactcataac ttaaactatc gccatcgggg tcaaacgcac ctgcattgtg
      481 cataaaggtt tgattgctgc aaccctggtc aataggtgga ttaagcaaaa cgggcgagtt
      541 attaaatcct aaaaacggat taatctgcaa aagcgtttca atataaaaac tggtgtttat
      601 actattgggt atattaataa caccgccatt acggttcata tcctcgcagt gaagtttata
      661 aattcctgct gctgcataat tatgatatcc tatgtatgtg tttttagtta tatcggttcc
      721 aaccgatgcc tgatttacac gcgaaactgt atcaaaagta ttatcgcccc aatgcagttc
      781 aagtacaggc ctgtcaatag cgctgctggt tttggtataa gttacaatag tggcttcgta
      841 ataaaacaat gatatttgcc tgtaagttat ttcgcctgca cggttatgtg ttgcttgtgc
      901 aatcagacta taaaacccaa aaaatatgat tagtaatttt ttcatgtata aggcaaacgt
      961 attaaatcat ttgcttgcta atttttaata tgcactaatt taataacgca gcaatattgc
     1021 aaagtaatat gggttattgc ctgcattaca aatcaattga tggcaagatg gtaaaaaatg
     1081 gcaatttgat tatgatgtaa gttactatca gttgctactt ataaaaccta aaccttacaa
     1141 gcagtaccta atttaataat taataacctg ttgctccatg ttttgcggca ttgcacgaaa
     1201 ctgatattgc tgattgcgca attgcggttc gaaacctgct tcggcaatag catcctgaat
     1261 accttgtgct gtaaagcgat gtggcgcacc ggctgcactt actacatttt cttctatcat
     1321 aatgcttcca aaatcgttgg cgcctgcatg caggcatatt tgagcggtgg ccttgcctac
     1381 ggtaagccag cttgcctgta tgtttttaat attgggcaac atgatgcggc ttaaagcaat
     1441 catgcgcaca tattcatcag cagttactgc attacgaata ccct
//
LOCUS       DAOMAA010000625         1483 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_100947_length_1483_cov_3.628151, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000625 DAOMAA010000000
VERSION     DAOMAA010000625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100947_length_1483_cov_3.628151"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="PLO59_08290"
     CDS             <1..244
                     /locus_tag="PLO59_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019942159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HQV01145.1"
                     /translation="TEIDFNFENRPYEFIDLETGQSIKLHSNLVSASYKKAVAQFETE
                     LKLKCAQYRIDFVEADIAKGYHQVLMPYLIKRGKMN"
     gene            343..419
                     /locus_tag="PLO59_08295"
     tRNA            343..419
                     /locus_tag="PLO59_08295"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:377..379,aa:Asp,seq:gtc)
     gene            complement(998..>1483)
                     /locus_tag="PLO59_08300"
     CDS             complement(998..>1483)
                     /locus_tag="PLO59_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HQV01146.1"
                     /translation="DSLNMALVMNLKGSLKDVNDEDVNVRVEGSAVFINISDKMLFKS
                     GSYEISSNASQVLGKVAQVLNGQPDMQFMVEGHTDNKPIKTPVIKDNWDLSAMRASAV
                     VRVLQKNYKIDPSRMIAAARSEYVPIAANNTIEGRTTNRRIRIVILPQLDQFFKLMEK
                     K"
BASE COUNT          471 a          289 c          234 g          489 t
ORIGIN      
        1 aactgaaatt gatttcaatt tcgaaaaccg cccttatgaa tttattgacc ttgaaaccgg
       61 acaaagcata aaactgcata gtaatctggt tagcgcttca tacaaaaaag cagtggcaca
      121 atttgaaacc gaactgaagt taaagtgtgc acaataccgt attgactttg tagaagccga
      181 catagcaaaa ggctatcatc aggtacttat gccctatttg attaaaagag gtaaaatgaa
      241 ttaggagcag gtccttaatt ctgctaaaaa taattttgct agatatattt ataattatac
      301 ttttgcgcac cgtttaaaaa acatctgttc ttaaacaaaa ttggttcggt agttcagctg
      361 gttagaatac atgcctgtca cgcatggggt cgcgggttcg agtcccgtcc ggaccgccaa
      421 aaatttttcc tctgctttta gcagaggttt ttttttgcct tttcttttga gcaatttcca
      481 agttcttatc aatttgtttt cttgttttta gctttttaat aaacccacaa actttttaat
      541 tataaggtgg ctaatcaaca agcagtagtt ttagaacaga aaaccgggca ctgctaattc
      601 tggtattgat ttacctaatt gttatagctg ttaaaataac aaattaataa tctaccggtt
      661 taatagactt tattaatttc taatgtaaaa aatatcctaa caaacacttt tgagttaata
      721 tctgttaacg gcaaaacgct taaccgtata tacacaatag gcagcaataa aaaatgcgtt
      781 ttatgggaaa gtactgccat aaacgaatta cgcatgcaag ccgaaaaagt atcagacagt
      841 attttcaata aatttcaaaa aagcagcctt atagctcagt aacatttaaa gcaccttaaa
      901 acatatttgc attagccata gctttcacaa cacttatctg caataaaaag gctgcatcca
      961 tagtatgaat gcagcctttt ttattaatgc aacttgctta tttcttttcc atcaatttaa
     1021 aaaactgatc taactgtggt agtattacaa tacgtattct gcgattggtt gtgcgaccct
     1081 caatagtatt atttgctgct atgggtacat actcgctacg agctgcagca atcatacgac
     1141 ttggatctat cttatagttc ttttgtaata cacggacaac agcactggct cgcatagcac
     1201 ttaaatccca gttatcctta attactggcg ttttgatagg cttattatct gtatgacctt
     1261 caaccataaa ctgcatatca ggctggccgt ttaatacttg ggctacctta cctaaaacct
     1321 ggctggcatt actcgaaatt tcatagctgc cacttttaaa taacatctta tcgcttatat
     1381 ttataaacac ggcactgcct tcaactctta catttacatc ttcgtcattt acatctttta
     1441 gcgagccctt taaattcatt accagggcca tgtttaatga atc
//
LOCUS       DAOMAA010000626         1483 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_100965_length_1483_cov_3.032213, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000626 DAOMAA010000000
VERSION     DAOMAA010000626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100965_length_1483_cov_3.032213"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(335..>1483)
                     /gene="der"
                     /locus_tag="PLO59_08305"
     CDS             complement(335..>1483)
                     /gene="der"
                     /locus_tag="PLO59_08305"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017257087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HQV01147.1"
                     /translation="SLIDTGGYVHGSEDAFEGDIRKQVKIAIDEAFYIMFVVDVETGL
                     TDLDEEVAKILRKSKKPVFVVVNKCDNNKRLLDINEFYGLGLGDLYPVSAANGSGTGE
                     LLDALTAAFKQDLMPEVDVPKIAIVGQPNVGKSSLLNALIGEERSIVNPLAGTTRDTI
                     YTRYKSFGFDFYLIDTAGLRKKKNVHEDIEFYAVLRTIRAIEECDVCIFMIDAQLGLS
                     AQDLNIFHLIQTNRKGVVFVVNKWDLIEKDNHTVKTYTDAMHQKLAPFTDVPIIFTSV
                     TEKQRVLKSLELAIEVCQNRSKHISTSQLNKFMLPHVDSNPPPATKGKLISIKYITQL
                     PGKTPMFACFSNHPNYIKDSYKRYIENKMREHFNLSGVPIEIFFRESK"
BASE COUNT          434 a          280 c          283 g          486 t
ORIGIN      
        1 gccttgcagt tgcattgcca tcagcattaa aataaataag attttacttg cacaatagca
       61 aatttttgct gtgtgtgtgt gtgtgtgtgt gattttttca taattactac gtttttcata
      121 atagtataaa ttaaagggtt aagggaaact tgttttggaa gtggtaaaga tataaaagga
      181 gttgagagtt gaaagggggg ttagtggtta gttgttagtg tttagtggtc tgttgtcttg
      241 taaaggttaa ttggtaaaag ctaaccacta aaagctaacc actatccact aaccactaac
      301 cacctgcaat taaacaggga aaagccaatc aggcttattt cgattctctg aaaaatattt
      361 cgatgggtac acctgaaaga ttaaaatgtt cgcgcatttt gttttcaatg taccttttat
      421 aactatcctt tatatagttt ggatgattac taaaacaagc aaacatgggt gttttgcctg
      481 gtagttgggt aatatatttt atgctgataa gtttgccttt agttgccggt ggggggttac
      541 tgtcaacatg tggcagcata aatttgttga gctgtgaagt gcttatatgt tttgacctgt
      601 tttggcaaac ttcaatagcc agttctaacg attttaatac cctttgtttt tcggttactg
      661 atgtaaaaat aatgggcaca tcggtaaatg gtgctaactt ttgatgcatg gcatcggtat
      721 aggttttaac cgtgtgattg tctttttcaa tcaaatccca cttatttacc acaaacacaa
      781 cgcctttacg gttggtttga attaaatgaa aaatattaag gtcctgtgct gataacccca
      841 actgggcatc aatcataaaa atgcatacat cacactcttc aattgctctg atggtgcgca
      901 acacggcata aaattcaata tcctcatgca cgtttttctt tttgcgcaaa ccggctgtat
      961 caattaaata aaaatcaaat ccaaaacttt tataacgcgt gtaaatggta tcgcgtgttg
     1021 tacccgccag tggatttacg atactgcgct cttcgccaat cagtgcatta agcagtgatg
     1081 atttacctac atttggctgc cctacaatgg caattttggg aacatcaact tcgggcatta
     1141 ggtcttgttt aaaagctgct gttaatgcat ctaacagttc gcctgttcca ctaccattgg
     1201 cagcacttac ggggtataag tcgccaaggc ctaagccata aaattcattt atatcgagca
     1261 aacgtttgtt attatcacac ttatttacca ccacaaaaac gggcttcttc gacttgcgta
     1321 aaattttggc aacttcttcg tctaaatcag ttagtccggt ttcaacgtca acaacaaaca
     1381 tgatgtaaaa agcctcatca atggcaattt ttacctgctt gcgaatatca ccttcaaaag
     1441 catcctcgct tccatgcaca tagccaccgg tatcaatcaa gct
//
LOCUS       DAOMAA010000627         1480 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_101359_length_1480_cov_2.851930, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000627 DAOMAA010000000
VERSION     DAOMAA010000627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101359_length_1480_cov_2.851930"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="PLO59_08310"
     CDS             <1..832
                     /locus_tag="PLO59_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006988001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HQV01148.1"
                     /translation="EQLKDIDILVFDIQDVGVRFYTYISTLQYVMEACADAGIQVLIL
                     DRPNPNGFYIDGPVLDTAFASFVGMQPIPVVHGLTMGEYAQMLVGEKWLRSIQPCNLK
                     VIKCAGYNHQMYYRLPVKPSPNLPNINAVYLYPSLCFFEGTNVSVGRGTNKPFQVIGL
                     PDSTLRGYTFTPVKIPQVAEKPLYENQKCYGMDLTTAAFDVQKYKQLHLKWLIGFYRN
                     SKTQASFFNTFFDKLAGTDVLRKQIEAGFSEADIRMSWQKDLDNYKLIRNRYLLYDDF
                     "
     gene            complement(835..>1480)
                     /locus_tag="PLO59_08315"
     CDS             complement(835..>1480)
                     /locus_tag="PLO59_08315"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01149.1"
                     /translation="TVMGILPIGSGNGLARHLCLPFNWQSNINLLLHGKIVCIDTLRV
                     NKKLSVNISGLGFEGQVAADFSKRKSRGLWGYLVTIIKTAFSYQSFEATIQYDGYTHK
                     QTAISIAIANASQYGNNAVIAPEASLSDETGNLVLIQKPSLLQIILAIPAFFQGKIQS
                     KSYIQNIRFRKATIISTSVAIHIDGEPYGHTQSVEVEVVPASLRLLIPAALFKY"
BASE COUNT          456 a          287 c          282 g          455 t
ORIGIN      
        1 cgaacagcta aaagacattg atattttggt ttttgatatt caggatgttg gcgttcggtt
       61 ttacacatat atcagcacat tacaatatgt aatggaggct tgtgccgatg caggcataca
      121 ggtattaata ctcgaccggc ctaacccaaa tggtttttat attgatggcc cggtgttaga
      181 tactgcattt gcttcatttg ttggcatgca acccataccg gtggtacacg gcttaactat
      241 gggcgagtat gctcaaatgc ttgttggcga gaaatggctg cgcagcatac aaccctgtaa
      301 tttaaaagtt ataaaatgtg ccgggtataa tcaccaaatg tattacagat taccggtaaa
      361 accatcgcct aacctaccaa acattaatgc agtttactta tatccttctt tatgtttttt
      421 tgagggtact aatgttagtg tgggccgtgg tacaaataag ccttttcagg taattggcct
      481 gccagactct acgttacgcg gatacacttt tacaccggta aaaataccgc aggttgccga
      541 aaaacctttg tatgaaaatc aaaaatgtta cggcatggat ttaacaacag cagcctttga
      601 tgtacaaaaa tataagcaac tgcatttaaa atggctaatt ggcttttacc gtaacagcaa
      661 aacgcaggca tcatttttta atacattctt tgataaactt gccggcacag atgtattgcg
      721 aaaacaaatt gaagcaggct ttagcgaagc cgatattcgc atgagctggc aaaaggattt
      781 ggataattat aaacttatca gaaaccgtta cctattgtat gatgattttt aaaatcaata
      841 cttaaataac gctgctggaa ttagcaaacg taatgatgcc ggcactacct ctacttctac
      901 agattgcgta tggccgtatg gctcgccatc aatatgaatg gctacactgg tactgataat
      961 ggttgctttg cgaaatcgga tattttgtat atacgatttt gattgaattt taccttgaaa
     1021 aaatgcagga attgctaata taatttgcag gagtgatggc ttttgaatta aaaccaaatt
     1081 accggtttca tcacttaacg atgcttcggg cgctattact gcattattac catactgcga
     1141 tgcattggca attgcgattg aaattgctgt ttgtttatgc gtgtagccat catattgtat
     1201 ggttgcttca aaactttgat aggaaaatgc tgttttaatt attgtaacca aatagcccca
     1261 taatcccctg cttttacgtt tcgaaaaatc ggcagcaacc tgcccttcga aacctaagcc
     1321 actaatgttt acgcttagct ttttgtttac ccgtaatgta tcaatacaca ctatcttacc
     1381 atgcaaaagc aaatttatat tagactgcca attaaaaggc aaacataaat gacgtgctaa
     1441 accattgccc gaaccgatag gcaaaatacc cataaccgta
//
LOCUS       DAOMAA010000628         1478 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_101602_length_1478_cov_3.665495, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000628 DAOMAA010000000
VERSION     DAOMAA010000628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101602_length_1478_cov_3.665495"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..515
                     /locus_tag="PLO59_08320"
     CDS             123..515
                     /locus_tag="PLO59_08320"
                     /inference="COORDINATES: protein motif:HMM:NF027349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HQV01150.1"
                     /translation="MKNKNLMLAVLLLFLCNISIAQTDVITEISNGIKEANAEKISRY
                     FSANIDLTTDKGEEMYSKVQATGVLRDFFVRYPVRNYTILHKGQKDEAEYVVGTYTST
                     RHINFRVYFFIKQSGNTKILNDLRFEKE"
     gene            614..1450
                     /gene="nadC"
                     /locus_tag="PLO59_08325"
     CDS             614..1450
                     /gene="nadC"
                     /locus_tag="PLO59_08325"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HQV01151.1"
                     /translation="MDNLIQFIKNGLAEDIGPGDYSALACIPANAKGKVALKVKEAGV
                     LAGCQLAQEILKQYVVDYDYEPLMYDGQNMQPGDIAFTVTGATQKILTAERLILNCMQ
                     RMSAIATITRSFVDEIAGTQAKILDTRKTTPLCRTIEKWAVRIGGGYNHRFGLYDMIM
                     IKDNHVDFCGGIEKAIDNVANFQRANGLTLQVEIETRDINQVKKVLAHGGINRIMLDN
                     FNTVDLSEAVTLINNTYETEASGGIKLHNVRSYALTGVNFISVGALTHGVKSLDLSLK
                     AV"
BASE COUNT          523 a          228 c          282 g          445 t
ORIGIN      
        1 ttataaccat tttaaaattg gtgtttggta taggttatta ttaacaaacc tacgttcatg
       61 ttaaatttat ttttgctaca aataataaat ggcattggct taattattgt gatttttaca
      121 gcatgaaaaa taaaaatttg atgctggccg ttttacttct gtttttgtgt aatatttcta
      181 ttgcgcaaac cgatgtaata actgaaatat ctaacggaat taaagaagcc aatgcagaaa
      241 aaataagcag gtactttagt gctaacattg acttaactac cgataagggc gaagaaatgt
      301 atagcaaagt acaggcaaca ggtgtgttac gcgatttttt tgtacgatat cctgtacgca
      361 actacaccat attacataaa ggtcaaaaag acgaagccga atatgtggtt ggaacttata
      421 caagtacacg ccacattaat ttcagagttt acttttttat aaaacaaagt ggcaacacca
      481 aaatattgaa cgacctgagg tttgaaaaag aataaaaaaa aaccactctg atttgagtgg
      541 tttttttttg aatattggca gcctgcatac atagttaacg cttaaaaaaa caagattgca
      601 gctgattttt aaaatggata atctaataca atttattaaa aatggtctgg cagaagatat
      661 aggcccgggc gattatagtg ctttggcatg catacctgca aatgctaaag gtaaagtggc
      721 gcttaaagtt aaagaagccg gtgtattagc cggttgccaa ttagctcagg aaatactcaa
      781 acaatatgtt gttgattatg actatgagcc attaatgtat gatggacaaa acatgcaacc
      841 tggcgatata gcatttacgg taacaggagc tacccaaaaa atattaactg ccgaacgtct
      901 gatacttaac tgtatgcaac gcatgagtgc cattgccaca attacacgaa gctttgttga
      961 tgaaatagca ggtacacaag caaaaatatt agataccaga aaaactacac cactttgccg
     1021 tactattgaa aaatgggctg ttcgcattgg cggtggttac aaccacaggt ttggtttata
     1081 tgatatgatt atgataaaag ataaccatgt tgacttttgt ggtggtattg aaaaagctat
     1141 tgacaatgta gccaattttc aaagagctaa tggattaaca ttacaggtag aaattgaaac
     1201 cagagatatt aatcaggtta aaaaagtatt ggcacatggc ggtataaacc gtataatgct
     1261 tgataacttt aacactgttg atttatccga agccgtaacc cttattaata atacttatga
     1321 aaccgaagca tcgggcggca ttaaactaca taatgtacgt agttatgcac ttacgggtgt
     1381 aaactttatc tctgttggtg cgcttacgca tggtgttaaa agtttagatt taagtttgaa
     1441 agctgtttaa ttgaaaatgc ctgaaactaa taaaaaac
//
LOCUS       DAOMAA010000629         1476 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_101923_length_1476_cov_2.197748, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000629 DAOMAA010000000
VERSION     DAOMAA010000629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101923_length_1476_cov_2.197748"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1476
                     /locus_tag="PLO59_08330"
     CDS             <1..>1476
                     /locus_tag="PLO59_08330"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQV01152.1"
                     /translation="QAALDASLDTSATFKNELMGYRKQLATPYMTDTTVSQQILTEAY
                     ERLQKDVRASHILIRCNPDALPKDTVAAYTKIMRLRDLITKKKFDFAKVARDSSDDPS
                     AKENGGDLGYFTGMQMVYPFETVAYTTPIGQISMPVRTKFGYHIIKVADHRAAQGEIH
                     CAHIMIKVAPGVADNDTAAINAKTKINEIYQKVKAGEKFEDLAATYSEDKGSAKNGGA
                     LPWFGTGRMVPEFEKAAFALKTDGQISEPIKSSYGWHIIKRLEARGIPSFDEKKAELK
                     QQISRDSRNEASKDAMISKIKSAYKYKENIAAKDEFLKSLDSAIVAGEYSDTLAGKFN
                     KNLFSLGNSNYAQSDFAAYIVTHQTKRSSGDPYNVGNAMYQAYVNETCLAYEESRLED
                     KYPDFKNLMQEYRDGILLFDLTDKQVWSRAVKDTTGLETFYNSNKTNYMWPERLHAVI
                     YSCASESIASDVRKLLKKKTPATDILDKTNKESQLNLSVKEG"
BASE COUNT          524 a          288 c          295 g          369 t
ORIGIN      
        1 caggctgccc ttgacgcgtc tttagatact tcggcaactt ttaaaaatga acttatgggg
       61 tatagaaaac aattggctac accttatatg acagatacta ccgtgagcca acaaatttta
      121 accgaagcat acgaacgctt acaaaaagat gttagagcca gccacatttt aatacgctgt
      181 aaccccgatg cattaccaaa agataccgta gctgcatata ctaaaataat gcgtttgcgt
      241 gatttgataa ccaaaaagaa atttgatttt gccaaagtag cacgcgattc aagtgacgat
      301 ccatcggcca aagaaaatgg tggcgacttg ggctacttta caggcatgca aatggtttat
      361 cctttcgaaa cggttgccta tacaacaccc attggtcaaa tatcaatgcc tgtgcgtaca
      421 aagtttgggt atcacataat taaggttgcc gatcatcgtg cagcacaagg cgaaattcac
      481 tgtgctcata ttatgattaa agtagcgccc ggtgtagccg ataacgatac tgcagctata
      541 aatgccaaaa caaaaattaa tgaaatttac cagaaagtaa aagcaggcga aaaatttgaa
      601 gacttagcag caacctatag cgaagacaaa ggctcggcta aaaacggtgg cgctttacca
      661 tggtttggca ctggtcgtat ggtgccagag tttgaaaagg ctgcttttgc cctcaaaacg
      721 gatggtcaaa tatcagaacc cattaaatca tcttatggct ggcatattat aaaacgcctt
      781 gaagcgagag gcataccttc ctttgacgag aaaaaggctg aattaaaaca acagatttcg
      841 cgagacagcc gcaacgaagc cagtaaggat gctatgattt cgaaaataaa atcggcctat
      901 aagtacaaag aaaacatagc tgccaaagac gagtttttaa aatcgttaga tagtgccatc
      961 gttgcaggcg aatacagcga taccttagca ggaaaattca acaaaaactt atttagttta
     1021 ggcaacagta attacgcaca gtcagatttt gctgcataca tagtaacaca tcaaaccaag
     1081 cgcagcagtg gcgaccctta taacgtgggc aatgccatgt atcaggcata cgtaaacgaa
     1141 acttgtttag cttatgaaga aagtcgttta gaagataaat atcctgactt taaaaattta
     1201 atgcaggaat atcgcgatgg tattttacta ttcgatttaa ccgataaaca agtttggtcg
     1261 cgtgcagtaa aagataccac cggtttagaa acgttttaca acagtaataa aaccaactac
     1321 atgtggcccg aacgcttaca tgctgttata tatagttgcg catctgaaag tattgcatct
     1381 gatgtacgca aattattaaa gaaaaaaaca ccagcaacag atatattaga caagactaat
     1441 aaagagtcgc aattaaacct atcagttaaa gaagga
//
LOCUS       DAOMAA010000630         1475 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_101992_length_1475_cov_3.219014, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000630 DAOMAA010000000
VERSION     DAOMAA010000630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1475
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101992_length_1475_cov_3.219014"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="PLO59_08335"
     CDS             complement(<1..220)
                     /locus_tag="PLO59_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014019115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HQV01153.1"
                     /translation="MSNTENSLLLGIAQKFGTPVYVYDGNVILNQYEKITNAFASCKT
                     KIKYACKALNNQHILKLLQQAGCGLDVVS"
     gene            complement(279..352)
                     /locus_tag="PLO59_08340"
     tRNA            complement(279..352)
                     /locus_tag="PLO59_08340"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(316..318),aa:Arg,seq:tct)
     gene            537..1358
                     /gene="bamD"
                     /locus_tag="PLO59_08345"
     CDS             537..1358
                     /gene="bamD"
                     /locus_tag="PLO59_08345"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HQV01154.1"
                     /translation="MFKALNYFLCLIAVFALASCSKYQQLLKSSNYDEKYAAAIKYYE
                     KKDYYRSLQLLEEMIVIFRGTTRGENVYYYYAQSHYGLQDYISAAYHFENFVKTFPVS
                     KHAEECAYLSAYCSYLDSPVYSLDQQNSEQAIEQFQLFINHYPQSDKVETCNKLMDVL
                     RAKIETKAFNNAKTFYYSDDYKASASALNNLIRDYPGTAYKEEAMYLILKSYYLYARK
                     SIDTKKPERYKSVTEAHTALLEAFPESKYLKQANSIEADAKRESESLTKSANATN"
BASE COUNT          534 a          264 c          239 g          438 t
ORIGIN      
        1 tcgaaaccac atctaagcca cagccggcct gctgcaatag ttttaaaata tgttggttgt
       61 ttaaagcttt acatgcgtat ttaattttgg ttttgcacga tgcaaatgca ttggtaattt
      121 tttcatattg attcaaaatc acattgccat catatacata taccggtgtg ccaaattttt
      181 gtgcaattcc taaaagcaga ctgttttcag tgttcgacat ggattaaaaa tcaaatggag
      241 tggttttaaa aaaaattcaa aacttcaagg taaaaaagca gtcccactag gaatcgaacc
      301 tagaacctac agtttagaaa actgttgctc tatcctgttg agctatagga ccgttttaaa
      361 attttaaatg tacaatacac caaagtgcat tccaaatcgt tacttgatga ttcgagcgcg
      421 aaaaaattaa aataaatcaa gtaatacaac acttacttta taagcacatg cataaaaata
      481 tggttaacct gtacacaatg cataggcaaa ctatttgcaa ataatacatt cgcaccatgt
      541 ttaaagcctt aaattatttt ttatgcttga tagctgtatt tgcgttagcc tcatgcagta
      601 aatatcagca attactaaaa agctccaatt atgatgaaaa gtatgccgct gcaataaagt
      661 attatgaaaa gaaagactat taccgctcgc tgcaattgtt agaagaaatg attgtgattt
      721 ttagaggtac aactcgaggc gaaaacgtat attattatta cgctcaatcg cactacggcc
      781 ttcaggatta catatcggct gcctatcatt tcgaaaattt tgttaaaaca tttccggtta
      841 gtaaacatgc cgaagagtgt gcttatttat cagcctattg ttcttattta gattcgcctg
      901 tgtatagttt agaccagcaa aactctgaac aagctattga acaatttcaa ttatttatta
      961 atcattatcc gcaaagcgat aaggttgaaa catgtaataa acttatggat gttttaagag
     1021 ctaaaattga gactaaggcc tttaataatg caaaaacatt ttactattct gacgactaca
     1081 aagcatcggc atcagcatta aacaacttaa ttcgcgatta tcccggtact gcttataaag
     1141 aagaagctat gtaccttatt ctaaaatctt attatcttta cgcccgcaaa agcattgaca
     1201 ctaaaaaacc tgaaaggtat aaatcagtta ctgaagcaca tactgcccta ttggaagcat
     1261 ttccggaaag taagtattta aaacaggcca atagtatcga agcggatgca aaacgcgaat
     1321 cagaatcatt aaccaaatcg gcaaatgcca caaattaaat agtaagcatg caaaacaatt
     1381 caaaagaaaa ctcaattgta acacgcaact tgaacgaata cattgacgaa accggaaatt
     1441 tatacgagtc gttggtaata attgccaaac gaagt
//
LOCUS       DAOMAA010000631         1472 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_102450_length_1472_cov_2.279464, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000631 DAOMAA010000000
VERSION     DAOMAA010000631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102450_length_1472_cov_2.279464"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1472
                     /locus_tag="PLO59_08350"
     CDS             <1..>1472
                     /locus_tag="PLO59_08350"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HQV01155.1"
                     /translation="ITSPVLSVFFMDTLTGFIGSDGGILKKTTDGGSTWSSYPLPSYN
                     NPVLDIIFSDSLNGFFITTYDFYRTIDGGITWSKVGQELNKVSYLSKDTLVAVGDDVF
                     RKSFDGGYTWTETHPFNRIHMYDMDFVDKDTGYASGYFGAIFKTINGGNNWTKQTTNS
                     SALFSGIHFFNYNKGIAVGSNYSIARTSNGGLNWVVDSFANGSLYGYYDVFFVNDSLG
                     YICGSQGKIARSTNGGTSFTTLTTGTTKYLLNILFLNPAKGFAVGESGTLLQTLDSGN
                     TWTKINLGTSLYLPSIAFADSMHGYVGTEYGDFYVTTDGGLTWKGKLFNAGAINDIAF
                     FDAYNGYTVGELDYKAKYDPLKAFNGYNDWCKGGGFNIKTLVPGNVTIDTGNVFLIEM
                     DTIGNNFKHAMVLGAVASDTSGQSVMFNIPQTLHTGYYQARLRATKTQPVNTSLTATV
                     RLFDSPSPTIFISNDTLYTAYHTGYSYQWRFNFAIIPNEH"
BASE COUNT          433 a          281 c          308 g          450 t
ORIGIN      
        1 attaccagcc cggtgctaag tgttttcttt atggatacgc ttacaggttt cataggcagc
       61 gatggcggta ttcttaaaaa aactacggat ggtggcagca cctggtcgtc ctatccgttg
      121 cccagttaca ataacccggt gctggatatt attttcagcg attcgttaaa cggatttttt
      181 ataactactt atgattttta tcgaaccatt gatggcggta taacctggag caaagtgggt
      241 caggaattaa ataaagtaag ttacctgagt aaagatacct tggtggccgt tggcgatgat
      301 gttttccgta aatcgttcga tggcggatat acctggactg aaacacatcc ctttaatcgc
      361 attcacatgt atgacatgga ttttgttgat aaagataccg gttatgcatc aggatatttt
      421 ggagccattt ttaaaaccat aaatgggggt aacaactgga ccaaacaaac cacaaacagc
      481 agtgcattgt ttagcggcat acattttttt aattacaata aaggaatagc cgttggcagt
      541 aattatagta ttgcacgtac cagcaatggc ggtttaaact gggtggttga ttcatttgcc
      601 aacggtagtt tatatggcta ctacgatgtg ttttttgtta acgactcact aggttatata
      661 tgcggttcgc agggcaaaat tgcgcgcagt acaaacggag gcaccagctt tacaacttta
      721 actaccggca ccactaaata cttattaaac attttatttt taaatccggc caaagggttt
      781 gctgttggcg aaagtggcac actattacaa acattagata gtggcaacac ctggacaaaa
      841 attaatttgg gcacatcatt atacttgcct tcaattgcct ttgccgattc aatgcatggt
      901 tatgtaggta ctgagtatgg cgatttttat gttaccactg atggcggctt aacctggaaa
      961 ggaaaactat ttaatgcagg cgccattaat gatattgctt tttttgatgc ctacaacggt
     1021 tataccgttg gcgaattaga ctacaaagcc aaatacgatc cgcttaaagc ttttaatggt
     1081 tacaacgact ggtgtaaagg tggtggcttt aatataaaaa cattggtgcc cggtaatgtt
     1141 accattgata ccggtaatgt gtttttgata gaaatggata ccataggaaa caactttaaa
     1201 catgcaatgg ttttgggtgc tgtggccagc gatacatcag gccaaagcgt aatgtttaat
     1261 atacctcaaa ccttacatac cgggtattat caggcgcgct taagagctac aaaaacgcaa
     1321 ccggttaata ccagtttaac cgctaccgtt cgattgtttg acagcccctc tcctaccatt
     1381 ttcataagca atgatacttt atatactgct tatcatacag gttatagtta ccaatggcgt
     1441 tttaattttg ccatcatacc caatgagcat aa
//
LOCUS       DAOMAA010000632         1470 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_102688_length_1470_cov_3.298940, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000632 DAOMAA010000000
VERSION     DAOMAA010000632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102688_length_1470_cov_3.298940"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1251
                     /locus_tag="PLO59_08355"
     CDS             <1..1251
                     /locus_tag="PLO59_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HQV01156.1"
                     /translation="MRGHEEASGTKYVPQELFDVWGRKDPVKNYEQYLLNEGVLDDAF
                     IEQIRSEIKTEIEAGLNITYAEPLPTSTTEKELQAIYAPHQQQNTMPLQSKSNDLRFL
                     DALTQAMEQSMEKFDNLVIMGQDIAEYGGVFKATAGFVERFGKGRIRNTPICESAIVG
                     AGLGLAINGYKAIVEMQFADFVTCGFNQIVNNLAKTHYRWGQNADVVVRMPTGAAVGA
                     GPFHSQSTESWFMQTPGLKVVYPAFPADAKGLLTAAIADPNPVMFFEHKALYRSVTQA
                     VPAEFYTLEIGKAALVTSGTEITIVTYGLGVHWAIDALKSNPQIQADLIDLRTLLPWD
                     KETVLASVKKTGKCIVLYETTYTAGVGAEIAAFVSEHAFSCLDAPVVRVASLDTPVPM
                     SEELEWNFLPKKRFSEALLKLHQY"
BASE COUNT          473 a          275 c          319 g          403 t
ORIGIN      
        1 atgcgcgggc acgaggaagc atcgggtact aaatatgtgc cccaagaatt gtttgatgta
       61 tggggccgta aagatcccgt aaaaaactat gaacagtatt tacttaatga aggtgtttta
      121 gatgatgcct tcattgaaca aataaggtca gaaataaaaa ctgaaataga agctggatta
      181 aatataacct atgccgaacc gctgccaacc tcaaccacag agaaagaact gcaagcaatt
      241 tatgcacctc atcagcagca aaacaccatg ccattgcaaa gcaaaagcaa tgatttaagg
      301 tttttagatg cgcttacaca agccatggaa caaagcatgg aaaagtttga taacctggta
      361 attatgggcc aagatattgc cgaatacgga ggtgtattta aagctactgc cggatttgtt
      421 gaacgttttg gcaagggccg catacgcaat acacctattt gcgaaagtgc aattgttggt
      481 gcaggtttgg gcttggctat aaatggctat aaggccattg tagaaatgca atttgccgac
      541 tttgtaactt gtggttttaa tcaaattgta aacaacttag ccaaaacaca ttatcgctgg
      601 ggccaaaatg ccgatgttgt agtacgcatg cctaccggtg cggccgtagg tgcagggcca
      661 tttcactcgc aaagtacaga gagttggttt atgcaaacac cgggtttaaa ggtagtgtac
      721 ccggcatttc cggccgatgc caaaggcttg ctaacggctg ccattgccga ccctaatccg
      781 gttatgtttt tcgaacataa agcgctgtat cgaagcgtta cacaagcagt gcctgccgaa
      841 ttttatacat tggaaattgg caaggctgca ttggtaacat caggcactga aataactata
      901 gttacttatg gcttaggtgt tcactgggcc atagatgcac taaaaagcaa tccacagata
      961 caagccgatt tgattgactt acgcaccttg ttaccttggg ataaggaaac ggttttagcg
     1021 agcgttaaaa aaacaggcaa atgcattgta ttatacgaaa ccacctatac tgccggtgtg
     1081 ggagctgaaa ttgctgcttt cgttagcgaa catgcattta gttgcttaga tgcccccgta
     1141 gtacgtgtag caagtttaga tacacctgta ccaatgagcg aagagttaga atggaacttt
     1201 ttacccaaaa aacgattttc agaagcatta ctaaaattgc atcaatacta atataaacat
     1261 taggtaaaaa aatgttagtg cgctttatac cgaaattatt attttagttt tgagcactta
     1321 tttaaatcac ttattaatcc aaacaaattt aaaatgaaaa gaagtttact tgcaatgggt
     1381 ttattgtgta gtagtttgtt tacacaagca caatggatca atcaaaatca agcatttact
     1441 tacgaaggct ttatggatgc cgtagaagtt
//
LOCUS       DAOMAA010000633         1470 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_102720_length_1470_cov_2.592226, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000633 DAOMAA010000000
VERSION     DAOMAA010000633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102720_length_1470_cov_2.592226"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..>1470
                     /locus_tag="PLO59_08360"
     CDS             52..>1470
                     /locus_tag="PLO59_08360"
                     /inference="COORDINATES: protein motif:HMM:NF012999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HQV01157.1"
                     /translation="MVKRSNNFNFVWQKHYKHPAIANNKLTVATDVKRVGNQYVLLTN
                     FEDTSAYNNVWIIKTDLNGDTIATKRFINYPFAFAIESHNTDAFVLATNATDSSGKYY
                     LKLVLLDTNFNEVATYNYRYKNFNEAITSFKRDTDGSYLIGLKTDSNNVRYAHSLKVA
                     PDFCITPKAFFNVGYNSGLQPNQSGVVIYNLSDAGIWDASTTITIDMGDGNILPFNTD
                     SLLHIYTTQGTYTVTVTITTVCGSSTYAQVITVACTGQPQYFTSTHNELLAQFNYQGA
                     QQNYNWTFGDGSTSTLQNPVHTYASPGTYYVCLNTVNACGPIELCDSVVVNCAAINIP
                     IASENKACMGTAITLNAGNNGSTFFWSDGSTNQILSVTLAGTYSVTVTNACGVSANLS
                     TNIIYSSLPLIDIGADTIMCIYDNVTISNLPGQSYNNNWLLNGFLQSTGNAFYFSQSS
                     IGSYNITLIADNNGCIDSVDRNI"
BASE COUNT          462 a          276 c          272 g          460 t
ORIGIN      
        1 aattatttat cttttgcatc cgaacccctt tcgcagctaa agcgtgcttc tttggttaaa
       61 cgttctaata atttcaattt tgtttggcaa aaacattata aacatcctgc cattgcaaac
      121 aataaactta cggttgcaac cgatgtaaaa agagtgggta accaatatgt attgcttacc
      181 aatttcgaag ataccagcgc ctacaacaat gtttggataa ttaaaacgga tttaaatggt
      241 gataccattg caaccaaacg atttataaat tatccatttg catttgcaat cgaatcgcat
      301 aatacggacg cttttgtatt agccaccaat gccactgata gcagcggcaa gtattatctt
      361 aaattggttt tattagacac caattttaat gaagttgcaa cctataatta cagatataaa
      421 aattttaatg aagcaataac cagttttaag cgcgatacag atggcagtta tttaatagga
      481 cttaagaccg atagcaacaa cgtgcgttat gcacattcgc taaaagtggc ccccgatttt
      541 tgtattaccc ctaaagcttt ttttaatgtg ggatataatt caggtttgca gcctaaccaa
      601 agcggtgtgg taatttataa tttatcggat gcaggcattt gggatgcaag tacaactatt
      661 actattgata tgggcgatgg aaacattttg ccattcaaca ccgacagttt gttacatatt
      721 tacactaccc agggcaccta tactgtaact gtaactatta caaccgtgtg tggatcaagt
      781 acgtacgccc aagttattac cgttgcttgt accggccagc cacagtattt tacaagtaca
      841 cataatgagc tactggcaca atttaattat cagggtgcac agcaaaatta caactggact
      901 tttggagatg gcagcacatc aactcttcaa aacccggtac acacatacgc cagcccaggt
      961 acttattatg tttgtttaaa caccgtcaat gcatgtggac caattgaatt atgtgactca
     1021 gtggtggtta attgtgcggc cataaacata cctattgctt cagagaataa agcttgcatg
     1081 ggtacggcaa taactttaaa tgcaggtaat aacggaagca catttttttg gtcagatggc
     1141 agtacaaacc agattttatc agttactctg gctggtacct atagtgtaac tgttacaaat
     1201 gcatgtggtg ttagcgccaa cttaagcacg aatattattt atagttcgct tcctttgatt
     1261 gatattggtg ctgatacgat aatgtgtatt tacgataatg ttaccatcag taatttaccg
     1321 ggccaatctt acaacaataa ctggttgctt aatggttttt tgcaaagcac aggaaatgct
     1381 ttttatttta gccaaagcag tataggtagc tataatatta cacttattgc cgataacaat
     1441 ggttgcattg attctgtgga tagaaacatt
//
LOCUS       DAOMAA010000634         1469 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_102853_length_1469_cov_2.602546, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000634 DAOMAA010000000
VERSION     DAOMAA010000634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102853_length_1469_cov_2.602546"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="PLO59_08365"
     CDS             <1..814
                     /locus_tag="PLO59_08365"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HQV01158.1"
                     /translation="LVSELAGKQVKVVLSGDGGDELFMGYGAYKWAKRLHRPYMPFAA
                     SGIKSILKQMPPRYKRVAGLFNFNHDFLPAHIFSQEQYLFSMHESKQMLFNSQPYHEM
                     FYNEFKSLNHDKKISAAAYQSLYDLCFYLPDDLLVKVDRTSMLCSIEARVPLLDRRLI
                     AFALNVPEPLKMHGNQMKIMLKQVLYKYVPKPILDRPKQGFSIPLQQWLQGPLHFLVE
                     DLLSQSAINNTGILNAKNVSALVKSWKNGNHFLYNRVWQLIVLQNFLKNNKR"
     gene            complement(817..>1469)
                     /locus_tag="PLO59_08370"
     CDS             complement(817..>1469)
                     /locus_tag="PLO59_08370"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HQV01159.1"
                     /translation="TTFKAVETTLIDIINIKHSGILSLGFVFALYFSTNGINAMLQSF
                     NKSYHGSSSSRPAWKQRVISLLLTIIIASLIIAAIAIMVIQQFAIKFIEQHNMLNNHL
                     YLMLINIGNWLVILALCFFAIACLYYFGSTRSKKFIFFSPGATFATLLIILTSIVFNY
                     YVSNFGNYNKLYGSIGTLIIVMLYIQFISMMLLIGYEFNVSVYNARKKHELQNKSN"
BASE COUNT          506 a          238 c          258 g          467 t
ORIGIN      
        1 tctggtttcg gaacttgcag gtaaacaagt taaggtagta ttatctggag atggaggcga
       61 tgaacttttt atgggctatg gcgcttataa atgggcaaaa cgattgcata gaccttacat
      121 gccttttgct gcatcgggta ttaaaagtat cctaaaacaa atgccgcccc gttacaagcg
      181 cgtagcaggg ctttttaatt tcaatcatga ttttttgcct gcccatattt tttcgcaaga
      241 gcaatactta ttcagcatgc atgaaagcaa acaaatgcta tttaattcgc agccatatca
      301 tgaaatgttt tataacgaat ttaaatcatt aaatcatgat aagaaaatat cggcagctgc
      361 atatcaaagt ttatatgatt tatgttttta cttgcctgat gatttgctcg taaaggtaga
      421 tagaaccagc atgctatgta gtattgaagc aagggtgcct ttactagaca gacgccttat
      481 tgcatttgca ttaaatgtac ctgagccatt aaaaatgcat ggcaatcaaa tgaaaataat
      541 gctgaaacag gttttgtaca aatacgtacc taaacccatt ctcgacaggc cgaaacaagg
      601 attttcaata ccattacaac aatggttgca agggcctttg cattttttgg ttgaagattt
      661 attaagccaa tctgctatta ataataccgg aattttaaat gcaaaaaatg tatcagcgct
      721 tgttaaaagc tggaaaaacg gtaatcattt tctgtataac agggtgtggc agttaatagt
      781 gttgcaaaat tttttaaaaa acaacaaacg gtaatattag ttgcttttgt tttgtagctc
      841 atgctttttt cgggcattat aaaccgatac attaaattca tatccaatca acaacatcat
      901 acttataaat tgaatgtaaa gcatcactat tattagagta ccaatagagc catatagctt
      961 gttgtaattg ccaaagtttg atacataata attaaataca atggaggtga gtataattag
     1021 cagtgttgca aatgttgctc cgggcgaaaa gaaaataaac tttttacttc gggtcgatcc
     1081 aaaataatac aaacatgcaa ttgcaaaaaa acacaaggct aatattacca accagtttcc
     1141 aatgtttata agcatcagat acaaatggtt gttaagcatg ttatgttgtt caataaattt
     1201 aatggcaaac tgttgaataa ccattatggc aattgcagca attataagcg atgcaattat
     1261 tattgtaagt agtaatgata taactctttg tttccatgca ggccgactac tactactacc
     1321 gtgatacgat ttattaaaac tttgcaacat ggcatttata ccattggtac taaagtaaag
     1381 cgcaaacaca aagcctaatg ataaaatacc actgtgttta atattgatga tatcaataag
     1441 tgtagtttct acagctttaa atgtggttt
//
LOCUS       DAOMAA010000635         1469 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_102871_length_1469_cov_2.410891, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000635 DAOMAA010000000
VERSION     DAOMAA010000635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102871_length_1469_cov_2.410891"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="PLO59_08375"
     CDS             <1..469
                     /locus_tag="PLO59_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HQV01160.1"
                     /translation="LTRCIPVNGKITARQKDVYNAVLRVMKAAAKMLLPGNTIEAYHQ
                     QVGLLMEAELIGLGLLKAEEVAQQNPAQPLYKKYFMHGTSHFLGLDIHDIGNKYLPMQ
                     AGMVFTVEPGIYIPEENLGIRIENDYLITTNGQIDLMQNIPIEADDIEMLMQG"
     gene            complement(508..>1469)
                     /locus_tag="PLO59_08380"
     CDS             complement(508..>1469)
                     /locus_tag="PLO59_08380"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HQV01161.1"
                     /translation="ANPWQLNVLYLLTVSNVYDCSNNTQTQTAQFALPDTAAPGDVLI
                     NEILFNPFTGGYDFIEIYNASQKIIDLKSLLIARYDLATDTVDQLYPVILTSTLLLPG
                     NYLVLTENTGDILSKYPLTIAGNLCKIINMPTLNDDAGKVALLNYQQKVIDVFRYSDD
                     FHYPLITDVEGISLERISLKQATQDSSNWQSAAKTVGFATPGYKNSQTIEHSSSNTTI
                     TVNPQIFSPDEDGYNDVLSIEYTLNEPGMVANVTVFDSRGRLVQYLARNTLMAESTTL
                     YWNGINTDNQKAPIGIYIIYSELFNTKGDVSKYKNTAVVAGKL"
BASE COUNT          435 a          275 c          292 g          467 t
ORIGIN      
        1 tttaacacga tgcataccgg ttaatggtaa gattactgca cgccaaaaag atgtatataa
       61 tgctgttttg cgtgtaatga aagctgcagc aaaaatgttg ctgcccggca ataccatcga
      121 agcttatcac caacaagttg gtttattaat ggaagctgag ttaataggtc tgggtttact
      181 gaaagcagaa gaggtggcac aacaaaatcc tgcacagcca ttgtataaaa aatactttat
      241 gcacggcact tcacactttt taggtttaga tatacatgac attggcaata aatatttgcc
      301 catgcaagcg ggtatggtgt ttaccgtaga acccggcatt tacatacctg aagaaaacct
      361 gggcattcgt atcgaaaacg attatctgat taccacaaac ggtcagattg atttaatgca
      421 aaacatacct attgaagctg atgatattga aatgttaatg caggggtaac agctttttct
      481 gcccctattt ttattacata gtacaaatta aagtttgcct gctactactg ccgtattttt
      541 atacttgctt acatcgcctt tggtattaaa cagctcgctg taaataatat aaataccgat
      601 gggggctttt tggttatcag tgtttatgcc attccagtac agtgttgtgc tttcggccat
      661 taaggtattg cgtgccagat attgcaccag gcggccgcga ctgtcaaaca ccgtaacatt
      721 tgcaaccatg cccggttcat ttaaagtata ttcaatacta agtacatcat tgtagccatc
      781 ttcatcgggc gaaaatattt gcgggtttac cgtaatagtt gtattactac tactgtgttc
      841 aatagtttgt gagtttttat aacccggtgt tgcaaagcca acagttttgg cagcactttg
      901 ccagttgcta ctatcttgtg tggcctgctt taatgaaatt ctttctaacg atataccctc
      961 aacatcggtt attaatggat agtgaaaatc atcactgtac ctaaacacat caattacttt
     1021 ttgttgatag ttaagcaagg ccactttacc ggcatcgtca tttaaagtgg gcatattaat
     1081 tatcttacat aaattacctg caatggttaa agggtactta cttagtatat caccggtgtt
     1141 ttctgttaac accaaataat tgccgggtag caatagtgta cttgttagta taacaggata
     1201 caactgatct acggtatcgg ttgccaaatc gtaacgcgca attaataacg attttaagtc
     1261 aataattttt tgcgaagcat tgtaaatttc tataaagtcg tatcctccgg tgaagggatt
     1321 aaaaagtatt tcgtttataa gcacatcacc gggcgctgcc gtatcgggca gtgcaaattg
     1381 cgcagtttgt gtttgggtat tgttgctgca atcgtaaaca tttgaaacag ttaataagta
     1441 tagcacatta agctgccagg ggtttgcaa
//
LOCUS       DAOMAA010000636         1469 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_102876_length_1469_cov_2.350778, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000636 DAOMAA010000000
VERSION     DAOMAA010000636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102876_length_1469_cov_2.350778"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1161
                     /locus_tag="PLO59_08385"
     CDS             <1..1161
                     /locus_tag="PLO59_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01162.1"
                     /translation="AMEIVTVVTKKQKQDFINLPVKIYSGDNNWIRPLDKDIEDVFDA
                     TTNKFFRHGSCVRFLLLSNNNCIGRVAAFINEKTAKREQYPTGGIGFFECIDDVNAAK
                     ILFDAAKKWLTERGMHAMDGPINFGEREAWWGLIVDGFSPPPYKMNYNKPYYKHLFEH
                     YGFKEYFKQLCFGLDPKKEVDAKLIARHAIIAANKDYRFECLQMNLMSKYAEDFKTVY
                     NSAWVKHGEGKALESHQVQAFFKKMKPVIDPKLVYYVYFKNEPVAFFINLPDINQLFK
                     KFNGRFGIVEKLKFITMLKMRSTKKIIGLVFGVVPQHQGLGVDSFMIVEASKIIRLQL
                     PYTEYEMQWIGDFNPKMVSVAQSLTTHQSRMLITYRKLFDEKRVFERHPIFS"
BASE COUNT          483 a          221 c          296 g          469 t
ORIGIN      
        1 gcaatggaga tagtaactgt agttaccaaa aaacaaaagc aagattttat taatttaccg
       61 gtaaagattt atagtggcga taataattgg atcagacctt tggataagga catcgaagat
      121 gtgtttgatg caacgactaa caaatttttt cgacatggaa gttgtgttcg gtttttactt
      181 ttatccaata acaattgtat aggccgtgtg gcagctttca ttaatgaaaa aacggccaaa
      241 cgcgaacaat atccaacagg tggcattggt tttttcgaat gtattgatga cgtaaatgca
      301 gcaaaaattt tatttgatgc agcaaagaaa tggctaactg aaagaggtat gcatgccatg
      361 gatggcccta taaattttgg cgaaagagaa gcctggtggg ggttaattgt tgatgggttt
      421 tcgccaccgc cctataaaat gaattataat aaaccttact ataaacactt attcgagcat
      481 tatggattta aagaatattt caaacaactt tgttttggtt tagatcctaa aaaggaggtg
      541 gatgctaaat taattgcccg tcatgcaatt attgccgcta ataaagatta tcgttttgaa
      601 tgtttgcaaa tgaacttgat gagcaaatat gccgaggatt ttaaaacggt atataacagt
      661 gcttgggtaa aacatggcga aggcaaagca ttagaatcac atcaggtgca ggcctttttt
      721 aaaaagatga aaccggtaat tgatcccaaa cttgtttatt atgtttattt caagaatgag
      781 cctgtagcat tctttatcaa cttacctgat ataaatcaac tgtttaaaaa atttaatggc
      841 cgttttggaa tagttgagaa attaaaattt ataaccatgc ttaaaatgcg gagcactaaa
      901 aaaattatag gtttagtttt tggtgtggta ccgcagcatc agggcttagg tgttgatagt
      961 tttatgatag ttgaagccag caaaattatt cgactgcaat tgccttatac tgaatacgaa
     1021 atgcagtgga ttggcgattt taatcccaaa atggtaagtg tggcgcaatc gcttacaacg
     1081 caccaaagcc gtatgttaat tacctaccgc aaattgttcg atgaaaagcg tgtatttgaa
     1141 agacacccaa tattttcata aaaaaaccac ctcgtttaaa aggtggttgc attatagtag
     1201 gctagtatgc ccccatatta gctttactat aatcaggttg ttgagctttg aggttggtat
     1261 aaggttgccg ggttaattat tttatttttt cctacgtgca atcttaaatt aaggtgtgtg
     1321 gtttttttca aattcaacgc tgctaaaatg caggcatagc ctcgtttata caataactaa
     1381 ttatagtgat atagatgcgt agccattaaa aaaaaacagc agccacattt tatggctgct
     1441 gtttttttaa tgttggtaac tgaaataac
//
LOCUS       DAOMAA010000637         1469 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_102890_length_1469_cov_2.141443, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000637 DAOMAA010000000
VERSION     DAOMAA010000637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102890_length_1469_cov_2.141443"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /locus_tag="PLO59_08390"
     CDS             complement(<1..875)
                     /locus_tag="PLO59_08390"
                     /inference="COORDINATES: protein motif:HMM:NF025104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HQV01163.1"
                     /translation="MDKQQKYYTIKYILADYLAAVAAWGLFYSFRKIYIESGKFGYQL
                     DINLDNHFWMGIIFIPIFWLLFYTLIGTYKNVLRRSRLREIGETLYVSIMGVLLIFFA
                     LLLDDTIVNYVSYYETIFALFAMHFLFTASLRFILSTGVARKIRNRTIGFNTLLIGSN
                     LKALNLFNEFESAKASYGNKFIGFINIDETNGHLFENKLPCLGSLLDARKIIADYKVE
                     EVIIAVESIEHEKVGRIVNELEDTKVTLKVIPDIYDILTGTVKLNAIFGALLLEINPE
                     IMPQWQQNAKRVFDIT"
     gene            complement(910..>1469)
                     /locus_tag="PLO59_08395"
     CDS             complement(910..>1469)
                     /locus_tag="PLO59_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01164.1"
                     /translation="VRSVYPLYTVYDTTLNFEPGKTAIIKPRCTYNSNVVVNVVDDFD
                     SGTTKFVRDADSDTMITTTNNAFEGSFSGEFYLDTIRRRISISTLNKFVIPKGRINII
                     ELNYNCNNSFVVGLIRNYVGNSQRLEMIAVLPSGGKWRKMYISIVDAVNNLTADNYNI
                     YIYAQIDQGNTQAKVMLDNLKLCNN"
BASE COUNT          464 a          270 c          257 g          478 t
ORIGIN      
        1 gtaatatcaa aaacgcgttt ggcattttgt tgccattgcg gcattatttc ggggtttatt
       61 tcgagtagca gtgccccaaa aatggcatta agttttacag taccggttag tatatcgtat
      121 atgtcgggta taacctttaa ggttaccttg gtgtcctcta attcatttac aatgcgccct
      181 actttttcat gttcaattga ttcaactgct attattactt cttctacttt atagtcggca
      241 ataatttttc tggcatctaa caatgaacct aagcatggca atttattttc gaaaagatga
      301 ccattggttt catcaatatt aataaagcca ataaacttat tgccatagct ggctttggcg
      361 ctttcaaact cattaaataa attgagtgct tttaagttgc ttccaataag tagggtatta
      421 aagcctattg ttcggtttcg gattttacgg gcaactcctg tacttaatat gaaacgtaag
      481 cttgccgtaa acaaaaagtg catggcaaat aatgcaaata tggtttcgta atagcttaca
      541 taatttacaa tggtatcgtc taaaagcaat gcgaaaaaaa taagcagcac acccatgatg
      601 cttacatata aagtttcgcc aatttcgcgc aaccggctac gccttaaaac atttttatac
      661 gtaccaataa gtgtgtaaaa caataaccaa aaaatgggaa taaaaataat gcccatccaa
      721 aaatgattgt caagatttat atctaactga tagccaaatt tacccgattc aatataaatt
      781 ttacggaacg aataaaacaa accccatgcg gctacagcag ccaggtaatc ggctaaaatg
      841 tattttatgg tgtagtattt ttgttgttta tccatgcagt tagtccaata aagctaagtt
      901 gcaaaagtgt tagttgttgc aaagcttcaa attatcgagc attacttttg cttgtgtatt
      961 tccttgatca atttgtgcgt agatgtatat gttataattg tcggcagtta aattatttac
     1021 ggcatcaaca atactaatat acattttacg ccacttgcca cccgatggca gtacggcaat
     1081 catttctaaa cgttggctgt tgcccacata gtttcttatc aggcccacaa caaatgaatt
     1141 gttacaattg taattaagtt caataatatt tatacgtcct tttgggataa caaatttgtt
     1201 tagcgtagat attgaaatgc gcctgcgtat ggtatctaaa taaaactcgc cactaaaaga
     1261 gccttcaaaa gcattattgg tggttgtaat catggtatcg ctatcggcat cgcgcacaaa
     1321 cttggttgta cccgaatcaa aatcatcaac tacattaacc acaacattac tgttatacgt
     1381 acatcgcggc ttaatgattg cggttttacc gggttcgaaa tttaatgttg tatcgtaaac
     1441 agtatagagc gggtaaacgc ttcgtacgg
//
LOCUS       DAOMAA010000638         1468 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_102993_length_1468_cov_2.629866, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000638 DAOMAA010000000
VERSION     DAOMAA010000638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102993_length_1468_cov_2.629866"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="PLO59_08400"
     CDS             complement(<1..625)
                     /locus_tag="PLO59_08400"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HQV01165.1"
                     /translation="MKSNFIRHILQWAWLCMAVLCFSLNAHAQVEELDDQAAEDSMAV
                     ILLGEDDETDTLEWADSLQLGNTQIEFDNVFNYDFSHALSTDSLNFRAGLLYDLESNT
                     IVWQKDMNYAYPIASVSKVMTSLLVIEALNAGKVNWNDEIKLTSQRTIYTGRRRSRRK
                     KVITTTITYTLRDLLKMTMIESNNYAAQLIGKHTAGGNLDAFIVMMNQ"
     gene            complement(844..>1468)
                     /locus_tag="PLO59_08405"
     CDS             complement(844..>1468)
                     /locus_tag="PLO59_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQV01166.1"
                     /translation="YIINCTGLYCDKIALKEGVKPNMKIVGFRGDYYDLTEAGRHKVR
                     NLIYPVPNPEFPFLGVHFTRMVSGETECGPNAVFTFKREGYGKSDFDLADTADALFYK
                     GTWKLFYKHWRFGLDEYKRAFSKKLFLERLQRMLPGLTMDDLKPGRAGVRAMALDADG
                     NMIDDFKIEHKGNALHVLNAPSPAATASLAIGNAIRQMADERFAGLK"
BASE COUNT          447 a          297 c          251 g          473 t
ORIGIN      
        1 gctgattcat cataacaata aatgcatcta aattaccacc ggctgtgtgc ttaccaataa
       61 gctgtgctgc ataattgttt gattcaatca tggtcatttt aagcaaatcg cgtagggtgt
      121 aagttatggt tgtagtaata acttttttgc gcctgctgcg cctgcggccg gtatatatgg
      181 tacgttgtga ggttaatttt atttcatcat tccaatttac tttaccagca tttaaagctt
      241 caattacaag taagctagtc attacttttg ataccgatgc aatgggatag gcatagttca
      301 tatccttttg ccaaacaatg gtattgcttt ctaaatcata taaaaggcct gccctgaaat
      361 ttaaagaatc agtacttaat gcatggctaa aatcataatt aaatacatta tcaaattcaa
      421 tttgtgtatt gcccaactgc aacgaatcgg cccattctaa tgtatctgtt tcatcatctt
      481 cacctaataa aattacagcc atactatctt cggcagcctg atcgtcaagc tcttctactt
      541 gtgcatgcgc attaagacta aagcacaata ctgccatgca taaccatgcc cattgcagta
      601 tgtgtcttat aaagttcgat ttcatttgct tgcaagttta atcagaatag atggtttctt
      661 atttttagta accatctatt ttacacaata ttgtgttaat tgtaaataat gcgtaaagcc
      721 tttatttaca ggcatttcga aggcacataa aaaaagtaat tacaataaat tactttcata
      781 aattgtatat gcacttttta ataagttgct atttgcaagt ttttaaaaat aaaaatgtaa
      841 tcattacttg agtccggcaa agcgttcatc ggccatttgc ctgatggcat taccaatggc
      901 cagcgatgct gtagcagcag gcgatggtgc attaagcaca tgcaatgcat tgcctttatg
      961 ttcgatttta aaatcatcaa tcatattacc atcagcatct aatgccattg cacgcacacc
     1021 tgcacgaccc ggctttaaat cgtccatggt taagccgggt agcatgcgtt gtaatcgttc
     1081 taaaaaaagt tttttcgaaa atgcacgttt gtattcatct aagccaaagc gccaatgttt
     1141 ataaaacaat ttccaggtgc ctttatagaa taatgcatcg gccgtgtcgg ctaaatcaaa
     1201 atcggattta ccataacctt cgcgcttaaa tgtaaatacg gcattggggc cacattcggt
     1261 ttcgccacta accatacgtg taaagtgtac acccaaaaac ggaaactccg gattaggcac
     1321 cggataaatc aggttacgca ctttatgcct gcctgcttcg gtaaggtcat aatagtcgcc
     1381 tctgaaacca acaattttca tattgggttt aacaccttct ttcaatgcaa ttttatcgca
     1441 atacaaaccg gtgcagttaa ttatatag
//
LOCUS       DAOMAA010000639         1466 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_103261_length_1466_cov_2.685330, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000639 DAOMAA010000000
VERSION     DAOMAA010000639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103261_length_1466_cov_2.685330"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="PLO59_08410"
     CDS             complement(<1..409)
                     /locus_tag="PLO59_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01167.1"
                     /translation="MNIKKLIPALALACIAFIPAANAQDKIVEGKAIFDLEFPTEGMD
                     EQYKAMMPKEATIYFKEGKTRTEMDMSMMTMVNIYNDKDKTMTMLMDMMGKKTATKMD
                     IDKIDSDKKKYTVTKTDETTTIAGYKCYKAWVAD"
     gene            complement(467..>1466)
                     /locus_tag="PLO59_08415"
     CDS             complement(467..>1466)
                     /locus_tag="PLO59_08415"
                     /inference="COORDINATES: protein motif:HMM:NF013044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HQV01168.1"
                     /translation="YTDAAIFEKSKTDIFLKTWQFASLTQAAQLHGSNKPFTLLPGFL
                     DEPLLITNQNNQHQCLSNVCTHRGKILVENACMQNHIRCRYHGRKFDLNGNMLSMPEF
                     EDVKNFPAPTDHLTQLQLKQWLQMFFVAATPAMDFEVCIDPIRPFIKQLSTDYIYQPT
                     LSRDYLVQAHWALYCENYLEGFHIPFVHHDLNKTLDYSSYHTQCFKYSSLQIGYAQSG
                     YPTLPIQHNEKSIAALYYWMFPNTMLNFYPWGLSVNVVKPITPHLTRVSFICFAYPNA
                     DVSGQINALDKVEREDEAIVESVQKGIGSRFYKHGRYATKRETGTHHFHGLIAEFL"
BASE COUNT          404 a          262 c          283 g          517 t
ORIGIN      
        1 catcagcaac ccaagcttta taacatttgt agccggcaat agttgttgtt tcatccgttt
       61 tggtaaccgt atattttttc ttgtcactgt caattttgtc aatgtccatt ttggttgctg
      121 ttttttttcc catcatatcc ataagcatgg tcatggtttt atctttatcg ttataaatgt
      181 ttaccatcgt catcattgac atatccattt cagttcgtgt tttaccttct ttaaaataaa
      241 tggtggcttc tttgggcatc attgctttgt attgttcatc catgccttcg gttggaaatt
      301 ctaaatcgaa aatagctttg ccttccacta ttttatcttg cgcattagcg gccggtataa
      361 atgcgatgca tgctaatgcc agtgccggaa taagtttttt aatattcatc tgattgtttt
      421 tttaagattt aagattgcgc caatatatat ataaacggca tcagctttat aaaaattcgg
      481 caattaagcc atgaaagtga tgtgtgcccg tttcgcgttt ggtagcataa cgaccatgtt
      541 tgtaaaatct ggaacctatg cctttctgca cgctttcaac tatagcttca tcttcgcgtt
      601 caactttgtc taatgcattt atctgaccgc ttacatctgc atttgggtaa gcaaaacaaa
      661 taaacgatac acgcgtcaaa tgaggtgtta tgggtttaac cacatttaca cttaagcccc
      721 aaggatagaa attgagcatg gtgttaggaa acatccaata ataaagcgca gcaattgatt
      781 tttcattgtg ttgtataggc agtgtagggt agccactttg tgcatagcct atttgtaacg
      841 aactatattt aaagcattgt gtgtgatagc tgctataatc aagtgtttta tttaaatcgt
      901 ggtgtacaaa tggtatatga aaaccttcta aatagttttc gcaatacaac gcccaatgtg
      961 cctgtaccaa ataatcgcgc gatagggtag gttgatagat gtaatcggtt gaaagttgtt
     1021 taataaatgg acgtatcgga tcaatacata cttcaaaatc cattgccgga gtagcagcaa
     1081 caaaaaacat ttgcaaccat tgttttaact gcagttgtgt taaatgatcg gttggggccg
     1141 gaaaattttt tacatcttca aactcaggca tgcttagcat gttgccattt aaatcaaatt
     1201 tacggccatg gtaacggcat cggatgtggt tttgcataca agcattttct accagtattt
     1261 taccacgatg tgtacatacg ttacttaaac attgatgctg gttgttttgg tttgttatga
     1321 gtagcggctc atctaaaaat ccgggcagta gtgtaaatgg cttattactt ccgtgcagtt
     1381 gtgccgcttg ggttaaggat gcaaattgcc atgtttttaa aaatatatcg gttttacttt
     1441 tttcaaatat agctgcatca gtgtag
//
LOCUS       DAOMAA010000640         1462 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_103797_length_1462_cov_2.576404, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000640 DAOMAA010000000
VERSION     DAOMAA010000640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1462
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103797_length_1462_cov_2.576404"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="PLO59_08420"
     CDS             complement(<1..395)
                     /locus_tag="PLO59_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HQV01169.1"
                     /translation="MKEFRFAYESYLYLYAIVPVIILLFILWVYRKRKTLNRFGQSQI
                     IKPLFEKTSTTKPLTKFILSVVALSFLILAVCGPQLGSKLTEVKREGAELMVCLDVSN
                     SMLAQDLSPNRLTRAKYALEKMIDKLEGD"
     gene            complement(407..1399)
                     /locus_tag="PLO59_08425"
     CDS             complement(407..1399)
                     /locus_tag="PLO59_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HQV01170.1"
                     /translation="MAGVTFANPQFFWLLLLIPAMLTYHIYVIRKRRARITFSSTQIF
                     TYTKPTLRQRFIFLPLALRLIGTTALIIALARPQSSSSGQDVRTEGISICLAMDVSGS
                     MLAEDFKPNRIEAVKKVALDFIDGRPNDQIGLVIFSGESFTLCPITTDHAVLKNQLMR
                     ASSEMLQDGTGIGDGLATAIARVKDAKTKSKVIILLTDGVNNSGAIAPATAGEIAKTF
                     GIRVYAIGVGRNGTAPYPFKTPFGIQYQDVEVKIDEALLKEISAGTDAKYFRATNNNK
                     LRDIYAEIDKLERTKIEVTEFRRYTELYWIWAAIGCTCLITELLLRYTLLRSIP"
BASE COUNT          407 a          303 c          292 g          460 t
ORIGIN      
        1 tgatcacctt caagtttatc aatcattttt tcaagtgcat atttcgcccg ggttaagcgg
       61 ttaggactta ggtcctgagc cagcatacta ttgcttacat ccaaacacac catgagttcg
      121 gcaccttcgc gttttacctc agttaatttg gaaccaagtt gtgggccaca tacagccagt
      181 attaaaaatg aaagtgcaac tacgcttaaa ataaattttg tgagcggctt ggttgttgaa
      241 gttttttcga aaagtggttt tataatttgc gattggccaa agcgatttaa tgtttttctt
      301 ttccggtaaa cccaaagtat gaacaatagt attataaccg gtacaatggc gtacaggtac
      361 aggtaacttt cgtatgcgaa tctaaactct ttcaacggtt gattaattaa ggtatggatc
      421 ttaatagagt gtatcgtaac aatagctcgg taattaaaca tgtacaacca atggctgccc
      481 atatccagta taattctgtg tatcttctga attcggttac ttcaattttt gtacgctcta
      541 atttgtcaat ctcagcataa atatcgcgca gcttattgtt attagtggct ctaaaatact
      601 ttgcatctgt acctgccgat atttctttaa gcaatgcttc gtcaatcttt acttccacat
      661 cctgatattg aatgccaaag ggtgttttaa aaggataagg tgctgtgcca ttgcgtccca
      721 cgccaattgc ataaacacga atgccaaaag ttttggcaat ttcaccggcc gttgccggag
      781 ctatagcacc actgttattt acaccatcgg ttagcagaat aattacttta ctttttgttt
      841 tagcatcttt aacacgtgct atggccgttg ccaatccatc gccaatgcca gtaccgtctt
      901 gcagcatttc gctgctggca cgcatcagtt ggttttttaa aacggcatgg tcggtggtga
      961 tggggcatag tgtaaaactc tcgccactaa aaattaccaa ccctatctga tcgttggggc
     1021 gaccatcaat aaaatcaagt gcaacttttt ttacggcttc aatgcggttt ggcttaaaat
     1081 cttcggccag catactgccc gatacgtcca tggccaaaca aattgaaatg ccttcggtac
     1141 gtacatcttg cccactgctt gatgattgtg gacgtgccag tgcaattata agtgcagtgg
     1201 tgccaataag ccttagtgct aatggtaaaa aaataaaccg ctgccgtaat gttggttttg
     1261 tgtatgtaaa aatttgagtt gacgaaaaag taatacgtgc cctgcgttta cgtataacgt
     1321 atatgtgata cgttagcata gccggtatta ataacagcaa ccaaaaaaac tgtgggttag
     1381 caaaagttac gcctgccatc atattgtggc ctccttttta attaccggct ccatggtggc
     1441 ttctacaaaa gcaatagctt gt
//
LOCUS       DAOMAA010000641         1461 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_103910_length_1461_cov_3.022760, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000641 DAOMAA010000000
VERSION     DAOMAA010000641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1461
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103910_length_1461_cov_3.022760"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="PLO59_08430"
     CDS             <1..724
                     /locus_tag="PLO59_08430"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HQV01171.1"
                     /translation="TFQLLSSIMVFMGTAFMAAYMFTHFPFRYLGVKRGCSIWCFVLA
                     GLAMLLLTPFVNYTYKLNTMLPLPQWMETLENEAGTYTKAFLNMPNLTVLLYNMVILA
                     LVPAIGEELLFRGVIQRILYGMVGNVHWAIICAAVFFSSMHMQFLGFIPRLAMGMVLG
                     YLFYWSGSLWLSIAAHFINNAATVILAYYSHVLPFNQDTIGTGKDDALLLLVSVAMAW
                     LCLWTIKILCERKPTDSSIDVT"
     gene            complement(717..1196)
                     /locus_tag="PLO59_08435"
     CDS             complement(717..1196)
                     /locus_tag="PLO59_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017260153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HQV01172.1"
                     /translation="MAAKSRKNFQVDNLDLQILSLLMHDAFMPYTEIARKLIVSSGTI
                     HVRMEKLQKLGVVSGTHLIINPQKIGYDVCAFIGVFLDKGSHYNEAAKAFKQIPEIVE
                     LHYTTGLYSMFAKIVCRDTIHLREVLNERIQNVKGIHRTETFISLEESFRRQIKLVK"
BASE COUNT          409 a          240 c          297 g          515 t
ORIGIN      
        1 aacatttcaa ctattgtcgt ctattatggt ttttatgggc acggctttta tggctgctta
       61 tatgtttaca cattttccgt ttcggtattt aggggttaaa cggggttgta gtatttggtg
      121 ttttgtattg gcggggttgg ctatgttact actaacacct tttgttaact acacctataa
      181 actaaatacc atgttgccat taccgcagtg gatggaaaca cttgaaaacg aagccggcac
      241 ttacaccaaa gcctttttaa acatgccaaa ccttacggtg ttattgtata atatggtgat
      301 actggctttg gtaccggcta ttggcgagga gcttttattt agaggcgtta tacagcgcat
      361 attatatggt atggtaggca atgtacattg ggccataata tgtgcagccg ttttttttag
      421 cagtatgcac atgcagtttt tgggttttat accacgtttg gcaatgggta tggttttggg
      481 ctatttgttt tattggagtg gctcattatg gttaagtatt gcagcgcatt ttataaacaa
      541 tgcagctacg gttattttgg cctactattc ccatgtattg ccttttaatc aggatactat
      601 tggaaccggc aaggacgatg cacttttatt gttggtatct gttgcgatgg cttggctgtg
      661 tctttggact ataaaaatat tgtgcgaacg aaaaccaacc gacagcagta tagacgttac
      721 ttaactaatt ttatctgacg tctgaaactt tcctctaacg aaataaatgt ttcggtgcgg
      781 tgtatgcctt ttacattttg aatacgctca ttaagcactt cgcgtaagtg tatcgtatcg
      841 cgacaaacaa ttttggcaaa catgctgtat aagcctgtag tgtagtgcag ttctactatt
      901 tccggaattt gtttaaatgc tttagcagct tcattgtaat gcgaaccttt atctaaaaat
      961 acgccaataa aggcacatac atcataccca attttttgag gattaataat aagatgtgtg
     1021 cctgatacca cgcccagttt ttgaagtttt tccatccgaa catgaatggt accgcttgat
     1081 acaataagct tacgagctat ttctgtataa ggcataaaag catcgtgcat taacaacgat
     1141 agtatttgta aatctaaatt atcaacttga aaatttttgc gtgattttgc agccattgat
     1201 tatcataatt acaacaaatg tattatataa ttgataaaag tataataatt atattaaatg
     1261 tttgaaatat ttgcattact tatgaatatg cttttacatt tgtgccattg aaagtttgaa
     1321 aggtgcttca tacgcatcaa gggtaagtgt aagggggtta ctctgtagcg ggttcgagtc
     1381 cggctcaaac aacgagtttc ataagggttt atttattact taaaaaggct ttatcgtaaa
     1441 acggtaaagc ctttttggtt t
//
LOCUS       DAOMAA010000642         1459 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_104261_length_1459_cov_2.134615, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000642 DAOMAA010000000
VERSION     DAOMAA010000642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104261_length_1459_cov_2.134615"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="PLO59_08440"
     CDS             complement(<1..351)
                     /locus_tag="PLO59_08440"
                     /inference="COORDINATES: protein motif:HMM:NF014411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HQV01173.1"
                     /translation="MKRILIVVGTRPNFIKVTQFKKANAALGNPFHIAIVHTGQHYDS
                     KMADVFFTQFDLVPDYFLNISHASANTQMAEIMLRIEQTILEYKPSVVKVVGDVNSTF
                     AAALTANKCNVKIAH"
     gene            578..>1459
                     /locus_tag="PLO59_08445"
     CDS             578..>1459
                     /locus_tag="PLO59_08445"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HQV01174.1"
                     /translation="MPLVALTLLLALTGYGYFGALPTFEQVENPESSLATEIISSDTV
                     VLGKYYFQNRTNTPYHDLSTNITKALLATEDVRFYEHSGVDLKGLFRVLFKSVILQQD
                     AGGGSTITQQLAKNLFPRKKQKSKFSIVIIKMKEWITATRLERNYTKDEIMAMYFNTV
                     DFGHNAFGIKSAARTYFDKMPSQLNMQESALLVGMLRGPSYYNPVNHEQRAKLRRNTV
                     LELMLKNGFISEQQCDSIKKTKIELKYQREDHNAGIATYFRETLRLELNKWCAAHKKS
                     DGKSYNLYRDGLRIYTTI"
BASE COUNT          480 a          278 c          270 g          431 t
ORIGIN      
        1 atgtgctatt tttacattac atttattagc agttagcgca gccgcaaaag ttgaatttac
       61 atcgcccaca accttaacca cgctaggttt atactccaaa atggtttgct caatccgcaa
      121 cataatttcg gccatttgcg tattggcact tgcatgtgat atatttaaaa aataatcggg
      181 caccaaatca aattgcgtaa aaaaaacatc agccatttta ctgtcataat gctggccggt
      241 atgtacaata gcaatatgga atggattacc taaagcagca ttggcttttt taaactgcgt
      301 tactttaata aagttgggtc gtgtacccac cacaatcaat atccgtttca taaaataatt
      361 ttggcgaata tgataaaaat gtaattagca gcaggctgac ttttaacacc cataatttta
      421 ggtaaacaaa gccaaaatac cgtttaagat tgtggctttg cctatgcaac atcacaaaac
      481 tttatacttt tggcgccttc aaatatcata catgacagca gctaaatccg caaaagggtt
      541 caaaaaatat atcatctttt tctggctgtt atttgttatg cctttagttg cacttacttt
      601 attgttggcg cttacaggtt atggctattt tggcgcattg cccactttcg agcaagtcga
      661 aaatcctgaa agcagtttag ccaccgaaat tatttcatct gatactgttg tattgggaaa
      721 gtattatttt caaaaccgaa ccaatacacc ctatcatgat ttgtcaacca acataaccaa
      781 agcattattg gctaccgaag atgttcggtt ttacgagcat tctggcgttg acttaaaagg
      841 cctattcagg gttttgttta agtcggttat tttgcagcaa gatgccggag gtggaagtac
      901 cataacacaa caattggcaa aaaatttatt ccctcgtaaa aaacaaaaat caaaatttag
      961 catcgttatt attaaaatga aggagtggat tacagccaca cgtttggaac gtaattacac
     1021 caaggatgaa attatggcca tgtattttaa taccgttgat tttggacaca atgcttttgg
     1081 aataaaatcg gcagcgcgca cttattttga taaaatgcct tcgcaattaa atatgcagga
     1141 aagtgcattg ctggttggca tgttacgcgg gccatcatat tacaatccgg ttaaccatga
     1201 gcaacgcgcc aaactcagac ggaataccgt tttagaactg atgcttaaga atgggtttat
     1261 aagcgagcaa caatgcgatt cgataaaaaa aaccaaaatc gaattaaagt atcaacgcga
     1321 agaccataat gcaggtattg caacttattt tagagaaacg ttgcggcttg aactgaataa
     1381 gtggtgtgca gcgcataaaa aatcagacgg aaaatcatac aacctgtatc gggatggatt
     1441 gcgcatctac actaccatt
//
LOCUS       DAOMAA010000643         1457 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_104470_length_1457_cov_3.554208, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000643 DAOMAA010000000
VERSION     DAOMAA010000643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104470_length_1457_cov_3.554208"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1457)
                     /gene="uvrC"
                     /locus_tag="PLO59_08450"
     CDS             complement(<1..>1457)
                     /gene="uvrC"
                     /locus_tag="PLO59_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013998454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HQV01175.1"
                     /translation="FPRIFYTRKKIKDGSIYFGPYTSVKTLFVLLDFIKQIYQLRTCN
                     LQLTAQNIAQQKFKVCLEYHIKNCKGPCAGLQSKPDYDQGINEINELLKGNIHSVISH
                     FNTLMKNAAADYRFEEAQLFKEKIEVLEMYKSKSVIVSPNITNVDVFAIATQKDTAFV
                     NYLKVINGAIVQAQTIELKKKLDETDAELMTLAIAELRMRFDSDASEILVNVLPDMAL
                     PKVFVTEPKIGDKKHLVEMSYKNARYTLKEKLAQYDLLNPQHKTDRLLAQMQTDLRMP
                     VLPVHIECFDNSNFQGTDAVGAMSVFINGKPAKKEYRHFNIKTVTGPDDFASMQEIIY
                     RRYSRLLNDKLPLPQLIVIDGGKGQLSAALSSLEKLNLRGKISIIGIAKKLEEIYFPG
                     DSVPMYLDKKGETLKIIQQLRDEVHRFGITHHRNKRSKNFIKTQLDNIPGIGRVTAEM
                     LLSEFKSVKGVSTASLENLIACIGKSKAQIVYSYY"
BASE COUNT          397 a          275 c          282 g          503 t
ORIGIN      
        1 ataatacgaa taaacaattt gtgctttcga tttgccaata caggcaatta aattttcgag
       61 gcttgcagta ctaacgcctt ttaccgattt aaattctgac agcaacattt ctgctgttac
      121 cctacctatg cccggtatgt tatctaattg tgttttaata aaatttttac tgcgtttgtt
      181 acggtggtgg gtaataccaa agcgatgcac ctcatcgcgt aattgctgaa ttattttcag
      241 ggtttcgcct tttttatcta agtacatggg tacactatcg cccggaaaat aaatttcctc
      301 taattttttg gcaatgccaa ttatcgaaat tttgccacgc aggtttaatt tttctaaact
      361 actaagcgct gcactaagct ggcctttgcc tccgtcaata acaataagtt gtggtaaggg
      421 cagtttgtcg tttaataacc ggctataacg tctgtaaata atttcctgca tggatgcaaa
      481 atcatcgggg ccggtaacgg ttttaatatt gaaatggcgg tactcttttt tagcaggctt
      541 gccatttata aaaacgctca tggcacccac tgcatcggta ccctgaaagt ttgaattgtc
      601 gaaacactca atatgcaccg gcaaaacggg catgcgtaaa tcggtttgca tttgcgcaag
      661 caatctgtcg gttttatgtt gcggattaag caaatcgtat tgtgccagtt tttctttcag
      721 ggtgtaacgg gcatttttat agctcatttc aaccaaatgt tttttatcac caatttttgg
      781 ttcggtaaca aatactttag gcagggccat atcgggcaaa acattaacca gtatttcaga
      841 agcatctgaa tcgaaacgca tccgcagttc agcaattgcc aaggtcatta gttcggcatc
      901 ggtttcgtct aatttctttt taagttcgat ggtttgtgct tgcacaatag cgccattaat
      961 tacttttaag tagtttacaa aggcagtgtc tttttgtgtt gcaatggcaa acacatctac
     1021 attggtaatg ttgggcgata caattacact tttgctttta tacatttcaa gcacttctat
     1081 tttttcttta aacagttgtg cttcttcgaa acggtaatcg gcagcagcat ttttcatcag
     1141 tgtattaaaa tggcttataa cactgtgtat gttgccttta agcaattcgt taatttcgtt
     1201 aatgccctgg tcgtaatcgg gtttcgattg cagccctgca caaggtcctt tgcagttttt
     1261 aatatgatac tctaaacaaa ctttaaattt ttgttgtgca atattttgcg cagttagctg
     1321 aaggttgcac gtgcgcagtt gatagatttg ttttataaaa tcaagcaaaa caaaaagtgt
     1381 ttttaccgaa gtgtaaggcc cgaaataaat actgccatct tttatttttt ttcgggtgta
     1441 aaaaatacgc ggaaagg
//
LOCUS       DAOMAA010000644         1457 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_104476_length_1457_cov_3.160485, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000644 DAOMAA010000000
VERSION     DAOMAA010000644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104476_length_1457_cov_3.160485"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="PLO59_08455"
     CDS             <1..220
                     /locus_tag="PLO59_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01176.1"
                     /translation="QIHPLTVMDASLHSYMKMQPDEACDEISKLINEVKKYGGTFNSL
                     WHNDTISNLGIWQNWQAVFMHTLKQAAN"
     gene            223..1110
                     /locus_tag="PLO59_08460"
     CDS             223..1110
                     /locus_tag="PLO59_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01177.1"
                     /translation="MIRLIPHSEINKQLWDAVIDAAPQQNIYAYSWYLDIACPGWQAL
                     ILDDYDAIMPLPVNRKWGISYVYRPYFIQQLGVFSKPGYENTVMLFAKSLLNHFRFVA
                     YAFNNYNQAYITTHDKMLWQSNNNYVLVNQPVYNTNLRRNLKQACNHNLEILNMDERL
                     IVDLFRSNKGVQITNLKQQHYTTFIKICQAVKIHAYMHALGISFEQQPCAGMIIFEIK
                     NTHVFIFSATNNLAKQCKAMPFLIDYYLNKHKPLVFDFEGSNQSKLAQFYKSFGSTLQ
                     TYSMLKINTLPAPVKWLKK"
     gene            1200..>1457
                     /locus_tag="PLO59_08465"
     CDS             1200..>1457
                     /locus_tag="PLO59_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003000859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HQV01178.1"
                     /translation="MPHILCKQNSIVNHFIAELRNVDVQNDRMRFRRNLERIGEIAAF
                     EISKTFDYIQTEVTTPLGIATVSLMAEQPILATILRAGLPLH"
BASE COUNT          514 a          259 c          252 g          432 t
ORIGIN      
        1 gcaaattcac ccgctaacgg taatggatgc aagcctgcac agctatatga aaatgcaacc
       61 cgatgaagcc tgtgacgaaa tatcaaaact gattaatgaa gtaaaaaaat atggaggcac
      121 ttttaatagc ctatggcata acgatacaat ttctaacttg ggtatttggc aaaactggca
      181 agccgttttt atgcatacct taaagcaagc agctaattaa tattgatacg tttaatacca
      241 catagcgaaa ttaacaaaca gttgtgggat gcagtaattg atgcagcacc acaacaaaac
      301 atttatgcat atagttggta tttagacatt gcatgcccgg gctggcaagc tttgatttta
      361 gatgattatg atgcaataat gcctttgcct gtaaatcgca agtggggcat aagttatgtg
      421 tacaggccct actttataca acaattgggt gtatttagca aacccggcta tgaaaatacg
      481 gttatgcttt ttgccaaatc attattaaac cattttaggt ttgtagcgta tgcttttaac
      541 aattataatc aggcatacat tactacacat gataaaatgc tttggcaaag caacaacaat
      601 tatgttttag taaatcaacc ggtttacaat accaatttgc gcagaaactt aaagcaagct
      661 tgtaatcata atttggaaat tttaaatatg gacgaaagac ttattgttga tttgttccgc
      721 tcaaataaag gcgtacaaat aactaatctg aaacaacagc attataccac ttttattaaa
      781 atttgccagg cagttaaaat ccatgcctat atgcatgcat taggcatttc tttcgaacag
      841 caaccatgtg caggcatgat aatttttgaa ataaaaaaca cacatgtttt cattttttcg
      901 gcaacaaata atttggctaa acaatgcaaa gcaatgcctt ttttaattga ttattatttg
      961 aataaacaca agcctttggt ttttgatttc gaaggaagca accaaagtaa acttgcacag
     1021 ttctataaaa gttttggctc aacattacaa acttatagca tgctaaaaat taatacactg
     1081 ccggcacccg ttaaatggct gaaaaaataa atggatgcag tataaattga ttgtatttat
     1141 tgcaaattgc aagggtcaaa cacaatgatt ttatcagaga acaaataatt gcttaaaaca
     1201 tgccgcacat cctttgtaaa caaaactcaa tagttaatca ttttattgcc gaattacgaa
     1261 atgtggatgt acaaaacgac agaatgcgtt ttagaagaaa cctggagcgc attggcgaaa
     1321 ttgcagcatt tgaaatcagt aaaacgtttg attatatcca aaccgaagtt acaacacctt
     1381 tgggtattgc taccgtatcg ttaatggccg agcaacctat acttgcaacc atactcagag
     1441 ccggattgcc cttgcat
//
LOCUS       DAOMAA010000645         1456 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_104605_length_1456_cov_3.952891, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000645 DAOMAA010000000
VERSION     DAOMAA010000645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104605_length_1456_cov_3.952891"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="PLO59_08470"
     CDS             <1..619
                     /locus_tag="PLO59_08470"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HQV01179.1"
                     /translation="HSPFCERDIKLPSNLETHDVQGEFLIKIKKCGLIYPYVYQNHPF
                     DLVGYDGYCFPYSFSIFNFEPITGRIHMPPPIHQTFQGNNFVICSFVPRLYDYHPQAI
                     PAPYHHSNIDSDELLYYVAGDFMSRNDIQKGQITLHPAGIPHGPHPGAIERSIGKTET
                     NELAVMIDPFNPVKITEEAMKLEMRQYYHSWSKPQQKSNGQPHSI"
     gene            657..>1456
                     /locus_tag="PLO59_08475"
     CDS             657..>1456
                     /locus_tag="PLO59_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQV01180.1"
                     /translation="MDNLTEVKNFNYAETKKIFAGAADFLPILATDYVEFYVGNAKQA
                     AHYYKTAFGFQELAYAGLETGMRDRTSYVLQQDKIKLVLTTPLNNDEIASHLTKHGDG
                     IKVIALRVNDAYDAYAQTMSRGAKSYIEPYELHDESGVVRLSGIYTYGDTVHLFVERK
                     NYKGLFLPGYEKWQTEYSPIDTGLKYIDHMVGNVNLGEMEVWSKFYAEVMGFYNLITF
                     DDKDISTAYTALMSKVMTNGNGYIKFPINEPAKGLKKSQVQEYLDFYNG"
BASE COUNT          485 a          271 c          283 g          417 t
ORIGIN      
        1 gcactcaccg ttttgcgaac gcgatattaa attaccgtca aatcttgaaa cgcatgatgt
       61 gcaaggcgaa tttttaatca agattaaaaa gtgtggttta atttatccct acgtttatca
      121 aaatcatcca ttcgatttgg tgggatacga tggctattgc ttcccgtatt cattttcaat
      181 tttcaatttc gagcctatta caggtcgcat tcacatgcct ccgcccatac accaaacatt
      241 tcagggcaat aattttgtta tctgctcatt tgttcctcgc ttgtatgatt accatccgca
      301 agctataccg gcaccatatc atcacagtaa tattgatagc gatgaattac tttattacgt
      361 agcaggcgat tttatgagtc gtaacgatat tcaaaaaggt cagataacat tacacccggc
      421 tggtattccg cacgggccac atccaggtgc aattgaacga agcataggca aaacggaaac
      481 caacgaactg gctgtaatga ttgatccttt taatccggtt aaaattacgg aagaagctat
      541 gaaactcgaa atgcgtcaat attaccacag ttggtcaaag cctcaacaaa agtcaaatgg
      601 acaaccacat tcaatttaat tggctaacga aaacaaatca attaaaataa tctatcatgg
      661 ataacttaac tgaagtaaaa aatttcaatt acgcagaaac aaaaaaaata ttcgcaggtg
      721 cagcagattt tttacccata ctggcaaccg attatgttga gttttatgtg ggcaatgcca
      781 agcaagctgc acattattat aaaacagcct ttggttttca agagttggct tatgcaggct
      841 tagaaaccgg catgcgcgat cgaacaagtt acgtgttgca acaagacaaa atcaaactgg
      901 tattaactac acctttaaat aatgatgaaa tagcatcaca tttaaccaaa catggcgatg
      961 ggattaaagt tatcgcattg cgcgttaacg atgcttacga tgcctatgcc caaaccatga
     1021 gcagaggagc aaaatcgtat atcgagcctt atgaattaca tgatgaaagt ggcgtagtgc
     1081 gcttgtcggg tatttacacc tatggcgata ccgttcattt atttgtggag cgcaaaaatt
     1141 ataaaggctt gtttttgccc ggatatgaaa aatggcaaac tgaatatagt cctattgata
     1201 ccggactaaa atatattgat cacatggttg gcaatgttaa tttaggagaa atggaagttt
     1261 ggagtaagtt ttatgccgaa gtaatgggct tttataatct gataactttt gatgataaag
     1321 atataagtac ggcatacaca gcgttaatga gcaaggttat gactaatgga aacggataca
     1381 tcaaatttcc gattaatgaa ccggctaagg gtcttaagaa atcacaggta caagagtacc
     1441 ttgattttta caatgg
//
LOCUS       DAOMAA010000646         1456 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_104637_length_1456_cov_2.904354, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000646 DAOMAA010000000
VERSION     DAOMAA010000646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104637_length_1456_cov_2.904354"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="PLO59_08480"
     CDS             <1..347
                     /locus_tag="PLO59_08480"
                     /inference="COORDINATES: protein motif:HMM:NF021922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclomaltodextrinase C-terminal
                     domain-containing protein"
                     /protein_id="HQV01181.1"
                     /translation="QRTYAMQHGIAEEEAHNFISKLALWRQYRPAIQNGKLMQFVPED
                     GVYVYFRYDDKATIMMVMNANEKAITLNTKRFAERVGSYTKGYEVISNKTLNQIENIE
                     VEAMSAQIIELK"
     gene            369..1028
                     /gene="pgmB"
                     /locus_tag="PLO59_08485"
     CDS             369..1028
                     /gene="pgmB"
                     /locus_tag="PLO59_08485"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0046352 - disaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HQV01182.1"
                     /translation="MLQACIFDLDGVVVDTAKYHYLAWKRLANSFGADFNELQNEKLK
                     GVSRIQSLELILQWAGITLSETEKAVAAQQKNDWYLEYINQMQPNEILPGVAVFFEEL
                     KSNNIKIVLGSASKNAIHCLQKINMLHYFDAIVDGNVVSEAKPNPAVFITAAAKINVL
                     PVNAVVFEDAQVGIDAALNGGFFTVGVGQADVLSNAHLVIHTFKQINLQKLQAAFGCK
                     I"
     gene            1047..>1456
                     /locus_tag="PLO59_08490"
     CDS             1047..>1456
                     /locus_tag="PLO59_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008509376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HQV01183.1"
                     /translation="MKQYIKHHPWHIIEEGFDAHLNKISESLCSIGNGRMGQRANFEE
                     HYSGETLQGTYLAGVYYPDKTRVGWWKNGYPEYFAKVLNAPNIIGIDVTINGTRLDLA
                     HCNIKSFNRTLDMKAGLLNRNFVATMPNGVTVTV"
BASE COUNT          510 a          239 c          288 g          419 t
ORIGIN      
        1 agcaacgtac atatgcaatg caacatggta ttgcagaaga agaagcacat aattttataa
       61 gtaaattagc gctttggcgt cagtacaggc ctgctataca aaatggcaaa ctgatgcaat
      121 ttgtacccga agatggtgta tatgtttatt tcaggtacga tgataaagct accattatga
      181 tggtgatgaa tgcgaatgaa aaagcgatta cattaaatac aaaacgattt gccgaacgtg
      241 ttggcagtta cacaaaaggt tatgaagtaa tatccaacaa aacattaaac cagattgaaa
      301 atattgaagt tgaagctatg tcggcacaaa taattgaact taagtaaaat cacattaaaa
      361 cataataaat gcttcaagct tgtattttcg atttagatgg tgttgtagtt gatacagcca
      421 aatatcatta cctggcatgg aaaagattgg caaatagttt tggagccgac tttaatgaat
      481 tgcaaaacga aaaactgaaa ggtgtaagcc gcatccaatc gctagaactt attttacaat
      541 gggccggtat cactttatca gaaaccgaaa aagctgtagc agcgcaacaa aagaacgatt
      601 ggtatttaga atatataaat caaatgcaac caaacgaaat attgccgggc gttgctgttt
      661 tttttgaaga gttaaaaagt aataatatta aaattgtctt aggtagtgcc agtaaaaatg
      721 ccatacactg tttgcaaaaa attaatatgc ttcattattt cgatgctatt gttgacggca
      781 atgtggttag cgaagctaag cccaatcctg cagtgtttat tactgcagca gcaaaaatta
      841 acgtactacc cgttaacgct gttgtttttg aagacgctca ggtaggcatt gatgctgcct
      901 taaatggtgg attttttact gttggagtag ggcaagccga tgttttaagt aatgcacatt
      961 tagttatcca cacttttaaa caaattaatt tacaaaaatt acaagcagca ttcggttgca
     1021 aaatttaatt tagaattaat catcttatga aacaatatat taaacatcac ccctggcaca
     1081 ttatcgaaga aggttttgat gcacatttaa ataaaatcag cgaaagctta tgcagcattg
     1141 gcaacggccg tatgggacaa cgggctaact ttgaagagca ttacagtggc gaaacattac
     1201 aaggaactta cctggcaggc gtttattatc ccgataaaac gcgcgttggt tggtggaaaa
     1261 acggctatcc cgaatatttt gccaaagtgc taaatgcacc aaatattatt ggcattgatg
     1321 taactataaa tggtaccaga ttagatttgg ctcactgcaa tattaaatcg tttaaccgta
     1381 cgttagatat gaaagccggt ttgctaaacc gaaactttgt tgcaaccatg ccaaatggtg
     1441 taacagttac tgtaag
//
LOCUS       DAOMAA010000647         1456 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_104660_length_1456_cov_2.475375, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000647 DAOMAA010000000
VERSION     DAOMAA010000647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104660_length_1456_cov_2.475375"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..162)
                     /locus_tag="PLO59_08495"
     CDS             complement(<1..162)
                     /locus_tag="PLO59_08495"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HQV01184.1"
                     /translation="MNYYQILEVETTATAETIRQAYRKLVLKYHPDKNSSATAHEMFL
                     RIQHAYEVLS"
     gene            complement(189..1217)
                     /locus_tag="PLO59_08500"
     CDS             complement(189..1217)
                     /locus_tag="PLO59_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HQV01185.1"
                     /translation="MQKILIIYTGGTIGMTYDTDLQTLVPFDFGKIYDRIPELKRFNC
                     KIDFYTFHPLIDSSDMQPKKWVLIAKHIYKHYDEYSGFIILHGTDTMSYTASALSFML
                     ENLSKPVILTGSQLPLEAVRTDARTNLITAIEIVSTHKQYTDGIKEVCIFFDNELYRG
                     NRTEKYTSSKFDAFKSANFPILAEAGVHIIYHEDNFLRWSKKQKFRIDEKLNDHIALL
                     KLYPGITQNFIETVLKMPQLRGVVIESYGAGNAPTNKTFIDALQEAISSGIVMVNVSQ
                     CSGGSVELGKYESSTQLQQIGVISGGDMTTESALTKLMYLFGKGLRNTQVEKFLVKNL
                     RGELTVIS"
     gene            complement(1202..>1456)
                     /locus_tag="PLO59_08505"
     CDS             complement(1202..>1456)
                     /locus_tag="PLO59_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HQV01186.1"
                     /translation="GGVLTFKKSGLDEIVSGIDIKHLLLETDAPYLAPVPHRGKRNDP
                     AFILFVAKKLAELKNVTLQNVASVTTQNAINLFNLPCKKY"
BASE COUNT          404 a          283 c          266 g          503 t
ORIGIN      
        1 actcaatact tcataagcat gctgaatgcg caaaaacatt tcatgcgcgg tagcgctgct
       61 gtttttatcc ggatgatatt taagcaccag tttacggtat gcctgcctta tggtttcggc
      121 agtagccgtt gtttcaactt ccagtatttg gtagtaattc aaatgaaaat tgctttaaaa
      181 cctaattttt aagaaataac cgtgagttcg ccccttaaat ttttaaccag aaatttttcg
      241 acctgtgtat tgcgtaaccc ttttccgaat aaatacatca atttcgttaa tgccgattcg
      301 gttgtcatat cgcctccgct tattacacca atttgctgaa gctgcgtact gctctcgtat
      361 ttacccagct ccacactacc gccactacat tgcgaaacat ttaccattac gataccgctg
      421 ctaattgctt cctgtaaagc gtctataaat gttttattag ttggcgcatt gcctgcacca
      481 taactttcga ttaccacgcc acgtagctgc ggcattttta atactgtttc gataaagttt
      541 tgagttatgc ccggatatag tttcagcagt gcaatatgat cattcagctt ctcatctatt
      601 ctgaattttt gttttttact ccatctcaaa aaattatcct catggtaaat aatatgtact
      661 ccggcttcgg caagtattgg aaaattagcc gacttaaaag catcaaattt tgacgatgta
      721 tatttttcgg ttcggttacc acgatacaat tcattatcaa aaaaaataca aacttccttt
      781 ataccatctg tgtattgttt atgtgtactt actatttcaa ttgctgtaat taagttggta
      841 cgtgcatcgg tgcgtacagc ttctaaaggc aattgcgagc cggtaagtat aaccggtttc
      901 gataaatttt cgagcataaa acttaatgcc gatgccgtgt acgacatggt atcggttcca
      961 tgtaaaatga taaatccgga atactcatca taatgcttat aaatatgttt tgctattagc
     1021 acccattttt tgggttgcat gtctgacgaa tcgatgagtg gatgaaaagt ataaaagtct
     1081 attttacagt tgaatcgttt taattcaggt atgcggtcat aaatttttcc aaaatcaaat
     1141 ggtactaatg tttgcaagtc ggtatcataa gtcatgccta tggtacctcc ggtatagatt
     1201 attagtattt tttgcatggt aagttaaata agtttatagc gttttgagta gtaacgcttg
     1261 ctacgttttg cagcgtaacg tttttaagtt cggctaattt ttttgccaca aataaaataa
     1321 aggcagggtc gtttctttta ccacggtgtg gcacgggtgc caggtagggc gcatcggttt
     1381 cgagcagcaa atgttttatg tcaatacctg agactatttc atctaaacct gattttttaa
     1441 aagttagcac tccgcc
//
LOCUS       DAOMAA010000648         1456 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_104673_length_1456_cov_2.164168, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000648 DAOMAA010000000
VERSION     DAOMAA010000648.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104673_length_1456_cov_2.164168"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="PLO59_08510"
     CDS             <1..321
                     /locus_tag="PLO59_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019949296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQV01187.1"
                     /translation="ILVMHHKLPVLGFRIGDFIYVTDANYIAPQSKLLMQNADVIVLN
                     ALRREPHISHYTLTQAIEELQVLKPNKGYLTHISHQMGLHKQVSAELPAPINLAYDGL
                     TLEI"
     gene            356..790
                     /locus_tag="PLO59_08515"
     CDS             356..790
                     /locus_tag="PLO59_08515"
                     /inference="COORDINATES: protein motif:HMM:NF017853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HQV01188.1"
                     /translation="MLDNLINLVKEHAAEAIVNNPAIDNSKNDAAISETANSIFESLK
                     GQAASGNLQDVLNMFQGGTSNNALSSVIESNAIQTLASKFGIDATQAGQMVQTMLPGI
                     LNSLVNKTNDANDNSFDLQDIVTKIGGNSGGIGGMIGKLFGN"
     gene            complement(847..>1456)
                     /locus_tag="PLO59_08520"
     CDS             complement(847..>1456)
                     /locus_tag="PLO59_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01189.1"
                     /translation="IWCNVNCNKYGIYVTLLCYGFGLVLLHGALKFKTIQAHITVAFY
                     LLVLVQVAILFFLMIYKWKIILKNTSPWFLVGIHAFRAAFAVLLFCYYKNAKVPAALT
                     WLGFNYDILVGLSAVIIAYYLRLGVPLFTKKIFLIVWHFLGLVSILVLLVTIAFYGNN
                     VFTIGLLFLFPACLIPLIVALHVLGIIQSYKQWAYVLPVDYD"
BASE COUNT          567 a          259 c          222 g          408 t
ORIGIN      
        1 atactagtaa tgcatcataa actacctgta ttgggattta gaattggtga ttttatttat
       61 gtaactgatg ccaattacat tgcaccacaa tcaaaattat taatgcaaaa tgccgatgtt
      121 attgttttaa atgccttaag gcgcgaacca catatttcgc attatacgtt aacccaagct
      181 attgaagagt tacaggtatt aaaaccaaac aaaggttatt taacgcatat cagtcatcaa
      241 atgggtttac ataagcaagt aagtgccgaa ttacccgctc ctataaattt ggcttatgat
      301 ggcttaactt tagaaattta aatttaaaaa tttatcactt aaaacttata caaatatgtt
      361 agacaattta attaatctgg taaaagaaca tgccgcagaa gccattgtaa acaaccctgc
      421 aattgataat tcaaaaaacg atgcagcaat tagcgaaaca gctaacagta ttttcgaatc
      481 attaaaaggt caggcagctt cgggcaattt gcaggatgtg cttaatatgt ttcaaggcgg
      541 tacatcaaac aatgctttat cgtctgtaat tgaaagtaat gccattcaaa cattggcatc
      601 aaaatttgga atagatgcta cacaagccgg ccaaatggtg caaaccatgt tacccggtat
      661 tttaaacagc ctggtaaata aaaccaacga tgctaacgac aacagttttg acttacaaga
      721 tattgtaaca aaaattggtg gtaacagtgg tggtattggt ggtatgattg ggaaattgtt
      781 tggcaactaa tcaactaaaa acatagcaaa caaaaaagcc ctgtagttaa taattatggg
      841 gcttttttaa tcgtaatcaa cgggtaatac ataagcccat tgcttgtaac tttgaataat
      901 gcccaaaaca tgcaaagcaa caatgagcgg tattaaacaa gccggaaata aaaataataa
      961 ccctattgta aaaacattat tgccatagaa agctatggta accaacaaaa ctaaaatact
     1021 tactaaccct aaaaaatgcc atacgattaa aaaaattttt ttagtaaata acggcacacc
     1081 taaacgtaag taatacgcaa taataactgc cgataagccc accaaaatat cgtaattaaa
     1141 acctaaccac gttaaagcgg ccggcacttt tgcattttta taataacaaa acagtaaaac
     1201 agcaaatgcg gccctaaatg catgaatccc taccaaaaac caaggcgaag tgttttttaa
     1261 aattattttc catttataaa tcattagaaa aaacagtatc gccacctgaa ctaatactag
     1321 aagataaaat gcaacagtaa tatgtgcttg tattgtttta aattttaaag caccgtgcaa
     1381 taacactaac ccaaaaccat aacaaagtag tgttacatat ataccatact tattgcaatt
     1441 aacattacac caaatt
//
LOCUS       DAOMAA010000649         1455 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_104753_length_1455_cov_2.942143, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000649 DAOMAA010000000
VERSION     DAOMAA010000649.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104753_length_1455_cov_2.942143"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="PLO59_08525"
     CDS             <1..210
                     /locus_tag="PLO59_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01190.1"
                     /translation="KPGMWVKLKGSKTLGLLEHLQNNKAHILFGSFKTIADVQHLMPI
                     DENDNKRSDKKSKDKVVKASNPKAR"
     gene            complement(227..871)
                     /locus_tag="PLO59_08530"
     CDS             complement(227..871)
                     /locus_tag="PLO59_08530"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HQV01191.1"
                     /translation="MKKVVVVLVFLVSNILAQAQVDPKAQEILKSVSVKYKSFKTLSA
                     KFKVNVTDQKTKTNESQVGTVILKGNKYKLLLGAQEIISDGNIVWTYLKDANEVQLND
                     PKSDPNAISPTNMFTIYEKGFKSKFIGEKVVGGKAVQIIELVPEDTKRNFSKVQLTVS
                     KAEKYITQAIIYQKNGANISYAVQEFKPNIPADDIMFTFNKAKYPGCEEVDLRN"
     gene            complement(1034..>1455)
                     /locus_tag="PLO59_08535"
     CDS             complement(1034..>1455)
                     /locus_tag="PLO59_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013631929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HQV01192.1"
                     /translation="NGDMLLSTGADLIRLQCAFVDTAEVEKITDFIGQQRGYPDAFKL
                     PEYIDPNGEATMATDFSASDRDALFEEAAKIIVQHQQGSASLLQRRLKLGYNRAGRLV
                     DQLEAAGIIGAFEGSKAREVKVKTEAELMEMLHGQRV"
BASE COUNT          440 a          284 c          246 g          485 t
ORIGIN      
        1 aaacccggca tgtgggttaa gcttaaaggc agtaaaacat taggtttgct tgaacattta
       61 caaaacaata aagcacatat tttatttggc agttttaaaa ccatagccga tgtgcaacac
      121 ttaatgccta ttgatgaaaa tgataataaa cgttcggata aaaaatcaaa agacaaagtg
      181 gttaaagcat caaaccctaa agccagataa aatacatcaa cgtaaattag ttgcgtaaat
      241 caacttcttc gcagccgggg tacttggctt tgttaaatgt aaacataata tcatcggcag
      301 gtatattggg cttaaactct tgcacagcgt aacttatatt ggcaccgttt ttctgataga
      361 taattgcttg cgtaatatat ttttcggctt tactaaccgt aagttgtact ttactaaaat
      421 tacgctttgt atcttcaggt accagttcaa taatttgaac agccttacct cctactactt
      481 tttcgcctat aaatttcgat ttaaatcctt tttcataaat agtaaacatg ttggtaggcg
      541 atatggcatt tgggtcgctt ttgggatcgt ttaactgtac ttcattggca tcctttaaat
      601 aagtccaaac aatgttaccg tctgatataa tttcttgtgc gcctaaaagc agcttgtact
      661 tattgccttt taaaattacg gtaccaacct ggctttcatt tgttttggtt ttttggtcgg
      721 taacatttac tttaaacttg gcactaagtg ttttgaatga tttatacttt acacttacac
      781 tttttaaaat ttcttgtgct ttcgggtcaa cttgtgcttg cgctaaaata tttgatacaa
      841 gaaaaaccaa tacaactact acttttttca tgggtggaaa ttaatgaggg ctcaaaatta
      901 aacttttaaa tgaatgcact taaagcatgg tgcattacaa taagttttcc aaaaccgttt
      961 caacatgttt ttggctcata aaaaactcat taattaaaat gaaggccaaa aaaatatttt
     1021 ttaaaatata tgcttaaacc ctttgtccat gtagcatctc catcaattcg gcttcggttt
     1081 ttacttttac ctcgcgtgcc ttactgcctt caaatgcacc aataattccg gctgcttcta
     1141 actgatcaac taatcgtccg gcacggttgt aacccaattt taatctgcgt tgcagtaacg
     1201 atgccgagcc ttgctgatgt tgcacaataa ttttggcagc ttcttcaaaa agtgcatctc
     1261 tgtccgaagc cgaaaaatcg gttgccattg tggcttcgcc attgggatca atatactcgg
     1321 gtaatttaaa tgcatcgggg tacccacgtt gttgcccgat aaagtcggta attttttcta
     1381 cctcagccgt atcaacaaaa gcacattgca acctgattaa atcggcaccg gttgaaagca
     1441 gcatatcgcc attac
//
LOCUS       DAOMAA010000650         1455 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_104758_length_1455_cov_2.876429, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000650 DAOMAA010000000
VERSION     DAOMAA010000650.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104758_length_1455_cov_2.876429"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            225..497
                     /locus_tag="PLO59_08540"
     CDS             225..497
                     /locus_tag="PLO59_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01193.1"
                     /translation="MHAYAILQKDKIIYSGFIEQEVVATAQNSGYNFSGIHRPPNDKD
                     TCTLSYAEFVVPLAKAVQEVSEQNTALLHKIELVEIKYGLLDKRIK"
     gene            499..657
                     /locus_tag="PLO59_08545"
     CDS             499..657
                     /locus_tag="PLO59_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01194.1"
                     /translation="MLHLKILAALADFRTLFVKEPCRKKKPQSIVLVDYATIIVSLQA
                     HLDEIFSS"
     gene            complement(768..1142)
                     /locus_tag="PLO59_08550"
     CDS             complement(768..1142)
                     /locus_tag="PLO59_08550"
                     /inference="COORDINATES: protein motif:HMM:NF021525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2255 family protein"
                     /protein_id="HQV01195.1"
                     /translation="MHKEALLKYINTHSLIGIKAGNDRTAFLEIWMVVVDNRMFARSW
                     GMAEKSWYNSFLQDPLGQIKCGETIYSIKANIPADNYILKDKINEAYLNKYNSAHNAK
                     YAKGITQEPHISKTMEFIICES"
BASE COUNT          476 a          230 c          246 g          503 t
ORIGIN      
        1 atttatgaaa aattgcaggt tgacacacgt acagaagcga ttaacaaagt gtatgggagt
       61 catctcaatt cgcaaacatc aacttgaaat acacaaatta ctgtctggta ttaatccata
      121 cgtgcatgtt gtatgctaac actttaatgg tatttcaaca ccattgatgt gaaggtaagt
      181 ttgcatgata atttttaaat cattttcatg acaagcaaat caacatgcat gcttacgcca
      241 ttttgcaaaa agacaaaata atttattcag gctttataga acaggaagtt gtagcaacag
      301 cacaaaattc aggttataac tttagcggca ttcacaggcc gccaaatgat aaagatactt
      361 gcacgttaag ctatgccgag tttgttgtgc cattggctaa agcggtgcaa gaggtatcag
      421 aacaaaacac tgcattattg cataaaatag aattagtaga aataaaatat ggcctattgg
      481 ataaacgaat taagtaacat gctgcattta aaaattctgg ctgcacttgc agattttcgg
      541 acgcttttcg ttaaagaacc ctgccgcaaa aaaaaaccgc aatcaatagt tttggttgat
      601 tatgcaacca ttattgtttc cttacaagca catctggacg aaatttttag cagttaaaaa
      661 ccaacatttt ttagcgtttg aaaaacatca catcgttgtt ttataataag gagtaagctg
      721 atttttaaaa tggattgatt tatgaataat taataaaatt aaatcaatca ggactcacaa
      781 attataaatt ccatggtttt acttatgtgt ggttcctggg taataccttt ggcatattta
      841 gcattatgag cgctgttgta tttattaaga tatgcctcgt ttattttatc ctttaaaata
      901 taattgtcgg caggtatgtt tgctttaata ctgtaaatag tttcgccaca ttttatttga
      961 cctaaggggt cttgtaaaaa actattatac caactttttt ctgccattcc ccatgaccgc
     1021 gcaaacattc tgttatcaac cactaccatc cagatttcta aaaatgctgt tctgtcatta
     1081 cctgctttaa ttccaatcag gctgtgggtg tttatatatt taagtaaggc ttctttatgc
     1141 ataaactaca atggttattg taataatttt agggcagtaa ctattcgata tgtaagtagg
     1201 taaacagtat gtatgatttt gcgcaagcta ttgatactaa aaatgtttta accgctcttg
     1261 ataaactact gaagtgggac ttggctgata gttgtacgtt ttagttcttc aattttatag
     1321 tttgaaagca caagctttat tttgatattt atttaattat aaaaagcagt gttaagcaat
     1381 gcttagttaa tttttctgtc tttaatacga aattattttt ggggtttgta actatgtacc
     1441 attgcacctg ataaa
//
LOCUS       DAOMAA010000651         1454 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_104888_length_1454_cov_2.785561, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000651 DAOMAA010000000
VERSION     DAOMAA010000651.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104888_length_1454_cov_2.785561"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1128
                     /locus_tag="PLO59_08555"
     CDS             <1..1128
                     /locus_tag="PLO59_08555"
                     /inference="COORDINATES: protein motif:HMM:NF028132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01196.1"
                     /translation="EKYFNEQFGFRSTLVRLRNQMDYSLFKKVNANGAIIGKQNYLYE
                     HNYIKAYYGADAITDSLLIDQVKKLKYIQEALQQKGILFTVIFAPGKAAFYPEFIPDN
                     LKPNTYHTTNIERYLYQCKSLSVNHINLHSWFNYLKNKSPYPLYSKYGIHWSEYGRNL
                     AADSIINYVSTKLQQPLPHFSFGKPYTSTVMQGVDNDIADGMNLLFKMPEQTMAYQTI
                     SVDKKPNDVQPKLLTVADSYWWQMFNIHISEHIFSNGKFLFYNRECHTENQPMQDVGM
                     LNLKAEVLNSNVVLLLATDANLPNLGWGFINNLYKDLTTYSDTDKWANADYINRVNSM
                     KAYIKTQADWYNGVVKQAQEKNISVDSMLTINAAFTVDQQK"
     gene            1148..>1454
                     /locus_tag="PLO59_08560"
     CDS             1148..>1454
                     /locus_tag="PLO59_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01197.1"
                     /translation="MKKISKAFIILCIPVLLGMLFYKCSSTETKTDAWDYETNYDQYF
                     GFVPDDFRVIKTDDAHSGKFVTKMDTAFPFSLTLVKNFGQLTNKKAKAVEVQTWAKCA
                     "
BASE COUNT          525 a          267 c          252 g          410 t
ORIGIN      
        1 gaaaaatatt ttaatgagca atttggtttt agaagtaccc tggtaaggtt gcgtaatcaa
       61 atggactata gtttgtttaa aaaagtaaat gccaatggtg ccattattgg caaacaaaac
      121 tatttgtatg agcacaacta cattaaagca tattacggtg ccgatgccat aaccgatagc
      181 ttattgattg atcaggttaa aaaattaaag tacatacagg aagcactgca acaaaaaggc
      241 attttgttta cggtaatttt tgcaccgggc aaagccgcat tttatccgga gtttataccc
      301 gataatttaa aacccaatac ctatcacacc accaacatcg agcgttactt atatcagtgt
      361 aaaagcctat cggtaaacca cattaatcta cacagttggt ttaattattt aaaaaacaaa
      421 tcgccctatc cattgtactc aaaatatggc atacactgga gcgaatatgg ccgcaatctg
      481 gctgccgact caataattaa ttacgtttca actaaattac aacaaccact gccccatttt
      541 agttttggca aaccctatac atcaacagtt atgcaaggtg ttgataatga tattgccgat
      601 ggcatgaatt tgctttttaa aatgcctgaa caaacaatgg cttatcaaac catttcagtt
      661 gataaaaaac caaacgatgt acaacccaag ctacttacag tagctgatag ttattggtgg
      721 caaatgttta acattcatat ttcagaacac atttttagca atggcaagtt tttattttac
      781 aaccgcgagt gccataccga aaaccaaccc atgcaagatg ttggtatgct aaaccttaag
      841 gccgaagtat taaacagcaa cgtagtttta ttattggcaa ccgatgccaa tttacctaac
      901 ctaggttggg gctttataaa taatttatac aaagacctaa ctacgtatag cgataccgat
      961 aagtgggcaa atgccgatta tataaatcgc gttaacagca tgaaagctta cattaaaaca
     1021 caggcagatt ggtataatgg tgttgtaaaa caggcacaag aaaaaaatat cagtgttgac
     1081 agtatgctaa ccattaatgc agcatttact gttgaccaac agaaatagaa tcagaaaatt
     1141 aaatcaaatg aaaaaaataa gcaaagcgtt tatcatttta tgcatacctg tattgttggg
     1201 catgttattt tataagtgca gttcaaccga aacaaaaacc gatgcttggg attatgaaac
     1261 aaactacgac cagtactttg gttttgttcc cgatgatttt agagttataa aaacagacga
     1321 tgcacacagt ggaaaattcg taacaaaaat ggataccgct tttccattta gtttaacatt
     1381 ggttaaaaac tttggccaac taaccaataa gaaagccaaa gcggttgaag tacaaacctg
     1441 ggccaaatgt gcag
//
LOCUS       DAOMAA010000652         1453 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_104972_length_1453_cov_3.778970, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000652 DAOMAA010000000
VERSION     DAOMAA010000652.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104972_length_1453_cov_3.778970"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1453)
                     /gene="alaS"
                     /locus_tag="PLO59_08565"
     CDS             complement(<1..>1453)
                     /gene="alaS"
                     /locus_tag="PLO59_08565"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011584832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HQV01198.1"
                     /translation="MGDTGPCGPCSEIHIDLRSEADRKILDGKKLVNESHPQVVEIWN
                     NVFMEFNRKADGSLHKLPAQHVDTGMGFERLCMALQGKQSNYDTDIFQPLIQYISKHC
                     KIPYGQTERTDIAMRVMSDHIRAISFAIADGQLPSNNKAGYVIRRILRRAVRYQYAVL
                     NIQEPFLHKLVALLAQQFDGVFPELKSQQEFVMRVVLEEESSFLRTLETGIKRFEQYR
                     ESSIAGTFAFELYDTYGFPIDLTQLMAREKNMVVDMDGFNVCLEEQKNRSRAASVVDT
                     DDWVQLMHIEKSVFIGYDFLHADIRIARYRKVTAKQQTQFQLVFDQTPFYAESGGQVG
                     DTGYIESANERIDILDTQKENNLIIHVVASLPDQIDKLFKAQVNANRRSAINNNHSAT
                     HLMHAALRKVLGDHVSQKGSLVNDAATRFDFSHFAKVTNNELQVIEDLVNDKIRQNIE
                     LIEERAVPINEAKSRGAMALFGEKYGDFVRVITF"
BASE COUNT          400 a          323 c          264 g          466 t
ORIGIN      
        1 gaaagttatt acccttacaa agtcgccata tttttcgcca aacaatgcca tagcgcccct
       61 acttttagct tcgttaatgg gcactgcgcg ttcttctatt agttcaatat tttgacggat
      121 tttatcattt accaaatcct caattacttg taattcgtta ttcgtaactt ttgcaaagtg
      181 cgaaaagtca aaccgggttg ctgcatcatt taccaatgaa cctttttgcg aaacatgatc
      241 acctaatact ttgcgcaagg ctgcatgcat taaatgtgtt gcactgtggt tgttattgat
      301 agcgctgcgt ctgttggcat taacctgcgc tttaaacaac ttatctatct ggtcgggtaa
      361 acttgcaaca acatgaataa tcaagttgtt ttctttttgc gtatccaata tgtctatgcg
      421 ctcgtttgct gattcaatat agcctgtatc gcctacctgg ccgccacttt cggcataaaa
      481 gggtgtttga tcaaacacca gttgaaattg cgtttgttgt ttggccgtta cctttcgata
      541 acgcgcaata cgtatatcgg catgtaaaaa atcataaccg ataaacacac ttttttcaat
      601 gtgcataagt tgtacccagt catcggtatc aacaacggaa gcagcacgcg agcggttttt
      661 ttgttcctcc aaacaaacat taaaaccatc catatcaact accatatttt tttcacgagc
      721 cattaattgt gttaagtcaa ttggaaaacc atacgtatca tacagttcga aagcaaaagt
      781 cccggcaata cttgattccc tatattgttc aaatcgtttg atcccggttt ctaatgttct
      841 taaaaatgac gattcttctt ctaaaaccac acgcataaca aattcctgtt gcgattttaa
      901 ttcaggaaaa acgccatcaa attgttgcgc aagcaatgct accagtttgt gtaaaaaagg
      961 ctcttgaatg tttagaactg cgtattgata acgcacggcc ctacgcaata tgcgcctgat
     1021 aacgtagcca gctttattgt ttgaaggcag ttgcccatcg gctatggcaa atgaaatagc
     1081 acgtatatga tcactcatta cacgcatggc aatatcggta cgttcggttt gaccgtatgg
     1141 tattttacaa tgtttgctta tgtattgaat caacggttga aatatatctg tatcgtaatt
     1201 ggattgtttg ccttgcaatg ccatgcacaa ccgttcgaaa cccatcccgg tatcaacatg
     1261 ctgtgcaggt agtttgtgca agctgccatc agctttacgg ttaaactcca taaatacatt
     1321 attccaaatt tcaacaactt gtgggtggct ttcgttcacc agttttttac catcaagtat
     1381 tttccggtct gcctcgcttc gcaaatcaat atgaatttcg ctgcatggtc cgcaaggccc
     1441 ggtatcgccc atc
//
LOCUS       DAOMAA010000653         1451 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_105256_length_1451_cov_3.576648, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000653 DAOMAA010000000
VERSION     DAOMAA010000653.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105256_length_1451_cov_3.576648"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="PLO59_08570"
     CDS             complement(<1..398)
                     /locus_tag="PLO59_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01199.1"
                     /translation="MKKFTKLSLVVITICLMLLGLDLYFNNSSTKPHNGTLALARIDF
                     KGLDSLQAEQVVFYLKKQKAVNNVMCLKPYTSAIYTYDISQHTAAQIAQLFNATSPIT
                     GTLFKPTKLQLSQGCPLSANTFTYKLYKYFS"
     gene            743..1279
                     /locus_tag="PLO59_08575"
     CDS             743..1279
                     /locus_tag="PLO59_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011587127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HQV01200.1"
                     /translation="MKILKKILFIISALIGLILLSGLFIPNEYTVSNSLNIRKPQTEV
                     IDFVKLLDNQKHYSVWVMADKELNPTITGTDGTVGATQSWNSKMDEVGQGSQTITYMD
                     DKCIKIDLDFKRPFESKAKAEMLFEKISDNETTLTMKFYGVSPFPFNIMSFYMGRFFI
                     SNAQNDNLANLKKILESK"
     gene            1287..>1451
                     /locus_tag="PLO59_08580"
     CDS             1287..>1451
                     /locus_tag="PLO59_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010856589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like FloA family protein"
                     /protein_id="HQV01201.1"
                     /translation="MSIPFSALTIAAGILFVFMFLYFVPVGLWITAVFSNVRISIFDL
                     ITMRLRKVPPS"
BASE COUNT          488 a          242 c          233 g          488 t
ORIGIN      
        1 gagaaatatt tatataattt ataagtgaat gtatttgctg ataaaggaca accttggctt
       61 agttgtaatt ttgtaggctt aaataacgta cccgtaattg gcgaagtagc attaaacaat
      121 tgtgctatct gtgcagcagt atgttggctt atgtcgtagg tataaatagc tgatgtataa
      181 ggtttcaaac acattacatt gtttactgcc ttttgctttt tcaaataaaa aaccacctgc
      241 tcggcttgta aactatccaa ccctttaaaa tcaatacgtg ccaacgctaa ggtgccgtta
      301 tgtggtttgg tacttgaatt attgaaatat aaatctaacc ccaaaagcat taaacaaatt
      361 gttataacga ctaatgatag tttggtaaat tttttcatta caaagtatgt ttgtcataca
      421 tacgccataa aaaaaagttt ggttttattt acttaaaaaa aatttaggat tgattcttca
      481 agtgctctat tattttaatc gtacgcggca aataccattg tattataatc aatttcacaa
      541 tgcttaacca tttagtattg ttgctaataa tctttaaatc attttaaaaa aaaattcctt
      601 ccgattatta gttatcccaa agcgcaagtt caatatcacg aaggcctttt gtaaacttat
      661 tgttttacat tgcattagta aaagctcggt tttcaagtaa atgatttcat taaaatataa
      721 tccatactaa accctaaaac ccatgaaaat tttaaaaaaa atccttttta taattagcgc
      781 cctcattggg ttgatattgc tttcaggttt atttataccc aatgaatata cggttagcaa
      841 ctctttaaat atcagaaaac cccaaaccga agtaattgat ttcgtaaaac tgctcgacaa
      901 ccaaaaacac tacagtgttt gggttatggc tgataaagaa ttaaacccaa ccattaccgg
      961 taccgatggt acggtaggtg ctacacaaag ttggaatagt aaaatggatg aagttggcca
     1021 aggctcgcaa acgataactt acatggatga taagtgtatt aaaattgact tagattttaa
     1081 acgccccttc gaatcgaaag caaaagcaga aatgttgttt gagaagatat cggataatga
     1141 aacaacactt actatgaaat tttatggcgt atcgcccttc ccatttaata ttatgtcatt
     1201 ttacatgggc cgtttcttta tcagtaatgc acaaaatgat aatttagcca accttaaaaa
     1261 aatattagag tcaaaataat taaaccatgt caattccatt ttcagcttta accattgccg
     1321 ctgggatact atttgtattc atgttcttat actttgtgcc tgtaggtttg tggattacgg
     1381 cagtgtttag caatgtacgt atcagtattt ttgatttaat aacgatgcgc ttgcgcaagg
     1441 tgccgccatc c
//
LOCUS       DAOMAA010000654         1451 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_105301_length_1451_cov_2.727794, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000654 DAOMAA010000000
VERSION     DAOMAA010000654.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105301_length_1451_cov_2.727794"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1451
                     /gene="rpoB"
                     /locus_tag="PLO59_08585"
     CDS             <1..>1451
                     /gene="rpoB"
                     /locus_tag="PLO59_08585"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012781015.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HQV01202.1"
                     /translation="DELKVSKTALKGCVGRKLAARVLRTWIEDFVDEDTGEVVSITRT
                     EVVLERETIIAEEHIEAIIESGTKSIILHKEDANSTDYAIIYNTLQKDTSNNEKEAVE
                     HIYRQLRNAEPPDEETARGIIEKLFFSDKRYDLGSVGRYRINKKLGLQTAEDVRVLTK
                     EDIILIIKYLIELINSKADVDDIDHLSNRRVRTVGEQLYSQFGVGLARMARTIRERMN
                     VRDNEVFTPADLINAKTLSSVINSFFGTNQLSQFMDQTNPLAEITHKRRLSALGPGGL
                     SRERAGFEVRDVHYTHYGRLCTIETPEGPNIGLISSLCVFAKINSLGFIATPYLTVND
                     GKIDMENKISYLTAEEEDGKVIAQATAGVDEKGQFQTGKVKARFEGDFPIVEPKQLHL
                     MDVGPNQIASIAASLIPFLEHDDANRALMGSNMQRQAVPLLRPQAPIVGTGLEANVVR
                     DSRVLINAEYDGIVEYVDANEIHIRSNRSEDERL"
BASE COUNT          491 a          284 c          300 g          376 t
ORIGIN      
        1 gacgaattaa aagttagtaa aacagcattg aaaggatgcg taggccgtaa acttgcagca
       61 cgcgttttac gtacatggat tgaagatttt gtggatgaag ataccggtga agttgtttca
      121 attactcgaa ccgaagttgt attagaacgc gaaaccataa ttgccgaaga acatattgaa
      181 gcgataatcg aatcaggtac aaaaagcatc atcctacaca aagaagatgc aaactcaacc
      241 gactacgcaa ttatttacaa tacgcttcaa aaagatacct ctaacaacga gaaagaggcg
      301 gttgaacata tttaccgtca attacgtaat gccgaaccac ctgatgaaga aactgcacgc
      361 ggtattatcg aaaaattatt tttctctgat aaacgctacg acttaggttc tgttgggcgt
      421 tatcgtatca ataaaaaatt aggattacaa acggctgaag atgtacgcgt tctcaccaaa
      481 gaagatatta tcctaattat aaaatatcta atcgagctta ttaactccaa agctgatgtt
      541 gatgatattg accatttaag taacagacgt gtacgtactg taggtgagca attatactca
      601 cagtttggtg taggtttagc acgtatggca cgcaccatac gcgagcgtat gaatgtaaga
      661 gataacgaag tgtttacacc tgccgatttg ataaacgcta aaacattatc atcggtaatt
      721 aattcattct ttggtaccaa ccagttatca cagtttatgg atcaaaccaa tccattagcc
      781 gaaataaccc ataaacgcag attatctgca ctgggccccg gtggtttaag ccgcgaacgt
      841 gcaggtttcg aagtacgtga cgtacattac acgcactatg gtcgtttatg tacaatcgaa
      901 acacctgaag gaccaaacat cggattaatt tcatcgcttt gtgttttcgc aaaaattaac
      961 agcttaggct ttattgccac accttaccta accgtaaatg atggcaagat tgacatggaa
     1021 aataagataa gctaccttac tgccgaagaa gaagatggaa aagtaattgc acaagcaaca
     1081 gcaggtgttg atgaaaaagg acagttccaa accggaaaag taaaagcacg tttcgaaggc
     1141 gattttccaa tagtggaacc caagcaattg cacttaatgg atgtgggacc taatcagatt
     1201 gcatccattg cagcttcatt aattccattc ttagaacatg atgatgcaaa ccgtgctttg
     1261 atgggttcga acatgcaacg ccaggcagta ccattattac gcccacaagc gcctattgta
     1321 ggtacaggct tagaagccaa tgtagtacgc gacagccgtg ttttgattaa tgccgaatat
     1381 gacggtattg ttgaatatgt tgacgctaac gaaatacata tacgcagtaa cagaagcgaa
     1441 gatgagcgcc t
//
LOCUS       DAOMAA010000655         1450 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_105440_length_1450_cov_2.701075, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000655 DAOMAA010000000
VERSION     DAOMAA010000655.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105440_length_1450_cov_2.701075"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..368
                     /locus_tag="PLO59_08590"
     CDS             9..368
                     /locus_tag="PLO59_08590"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010854687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQV01203.1"
                     /translation="MLINSTLAERRPITEPERIAQMLAHANLIVTARLNNKLIGIARS
                     LTDFAFCTYMSDLAIDVEFQKQGIGKELIKQTTLAAPQAKLILLAAPKAVNYYPKIGM
                     VHHNHCYILDDINTLKM"
     gene            complement(482..>1450)
                     /locus_tag="PLO59_08595"
     CDS             complement(482..>1450)
                     /locus_tag="PLO59_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HQV01204.1"
                     /translation="KNLQRTYQLYNQFQFSGQNFFNTGIDYKLLLRNFNFFGEISRSQ
                     NGAMATINGVIMSVDPKLTFTLLYRNYGKKYEAIESNAIAESDNVNERGLYAGYSFKP
                     VRKISLTGYFDLFSYPWLRFAVNAPSHGFEYITQLTYKPNKQLEMYARYRQTNKQGNV
                     APAEDAPKIDYLVNNHQQNLRFNIAYKISKVLAIRSRVEWVYFKDGNQPLENGFLAFQ
                     DFIYKPLGKPLSITIRYAVFGTKSYNSRLYAYENDVYGAFSIPPLYGQGQRFYALLRY
                     HISRQVDLFLRYSQTVYYNQTTIGSGLDKIEGNSRNDIKLVLKMEL"
BASE COUNT          486 a          276 c          242 g          446 t
ORIGIN      
        1 ttacgcagtt gcttataaac tcaaccttgg ccgaacgaag acccataacc gaacctgaaa
       61 gaattgccca aatgttagcg catgcaaacc tgatagtaac cgcacgttta aacaacaaac
      121 tgattggcat tgccaggtcg ttaaccgatt ttgctttttg tacctacatg tcggatttag
      181 caatagatgt agaatttcaa aaacaaggca tcggaaaaga attaataaaa caaactacgt
      241 tagctgcacc acaagccaaa cttatattat tagcagcacc aaaggctgta aattattatc
      301 caaaaattgg catggtacat cataatcatt gttacatctt ggatgatatt aacacattaa
      361 agatgtaata actgactaca gtgaattgta actaatctat gtgttataag cattacaaaa
      421 gttgaaaaac gcatctgtta ttacaaacta ttatcaccaa taatgttacg agataaatga
      481 cttacaattc cattttcaaa accaacttaa tatcatttct actattgcct tctattttat
      541 ctaacccgct tccaattgta gtttggttat aataaacggt ttgtgaataa cgcaagaaca
      601 aatcaacctg acgcgaaatg tgatagcgca acaatgcata aaatctttgt ccctgtccgt
      661 aaagtggtgg tatagaaaac gcgccataca catcgttttc ataagcatac aaacgggagt
      721 tgtagctctt tgtaccgaaa acagcatacc ttattgtaat agacaatggc ttacctaaag
      781 gcttgtatat aaaatcctga aaggctaaaa atccattttc caaaggttga tttccatctt
      841 taaaatatac ccattcaaca cggcttcgga tagctaacac tttcgaaatt ttgtaggcaa
      901 tattaaaacg caggttttgc tgatgattat ttactaaata atcaatcttg ggtgcgtctt
      961 ctgcgggcgc aacattgcct tgtttattgg tttgcctgta gcgtgcatac atttctaatt
     1021 gcttgttggg tttataagtt agctgcgtta tatattcaaa accatgcgat ggtgcattaa
     1081 cagcgaaacg taaccatgga tagctaaaca aatcaaagta tccggtaagc gaaatttttc
     1141 gaaccggttt aaatgagtag cctgcataca aaccgcgttc gtttacatta tccgattcgg
     1201 caatggcatt actttcaata gcttcatact tcttaccata gttccggtat aataatgtaa
     1261 aagtaagttt tgggtcaaca ctcattatta cgccattaat agttgccatg gctccattct
     1321 gggagcgtga tatttcgcca aagaaattaa agttacgtag caacaatttg taatcaatac
     1381 ctgtattaaa aaaattttgt ccactaaact gaaactgatt gtaaagctga tacgtccgtt
     1441 gtaaattttt
//
LOCUS       DAOMAA010000656         1449 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_105550_length_1449_cov_3.657102, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000656 DAOMAA010000000
VERSION     DAOMAA010000656.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105550_length_1449_cov_3.657102"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            22..>1449
                     /locus_tag="PLO59_08600"
     CDS             22..>1449
                     /locus_tag="PLO59_08600"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HQV01205.1"
                     /translation="MKKIYAALLIACFTQASAQNNQNIPCITTEVMQELKDADPQIQK
                     NLNDLEFFTEKYIKEKHVKNADGTYQKVQGVVRVIPVVFHIIHEGGNENISIAQCQSQ
                     IDVLNADFRRLNADTTDTPGPFQAIGGDAEIEFRMAQLDPNGNCTDGVVRLFSTLTNN
                     ARNNVKSLSYWPSNKYLNVWVVKSIRGAQPPLQVAGFAQFPGGAAATDGIVICHQFLG
                     TIGTSTGSGEGRTTTHEVGHWLNLIHIWGDDAGACSGSDFVSDTPNQANLNQSNCPTF
                     PHTDACTPAGNGVLYSDYMDYTNGNCQNIFTVGQCARMNAALSSGTSQRNNLWSSTNL
                     VATGTNGTPAVPCAPIPSVVFGVRNICAGGNLTFNEYVYNGIPTSRDWVFTGGNPATS
                     TNASEVVTYNTPGTYNVTYTATNAVGSNTTTYAGIVNVLPTIGVKSVPYTDGFEGSFP
                     GFDYDVINGEGNFTWEQTSLAAATGT"
BASE COUNT          457 a          295 c          299 g          398 t
ORIGIN      
        1 gaaagaaaaa cgtattgtct catgaaaaaa atatatgctg cattgttaat agcatgcttt
       61 acgcaagcct cagcccaaaa caatcaaaat atcccttgca taaccactga agtgatgcag
      121 gaattaaaag atgctgaccc acaaattcaa aagaatttaa atgatttaga gttttttacc
      181 gaaaaatata tcaaagaaaa acatgttaaa aatgccgatg gtacctatca aaaagtgcaa
      241 ggggtagtaa gagttatacc tgtggttttt cacattatac atgaaggggg caacgaaaac
      301 atttcaatag cgcaatgcca aagtcaaatt gatgtattaa atgctgattt cagacgactt
      361 aatgctgata ctaccgatac accaggccca tttcaagcta ttggtggcga tgccgaaatt
      421 gaatttagaa tggcacaatt agatcctaat ggaaattgta ctgatggtgt ggtacgcttg
      481 ttttctacac ttacaaacaa tgcacgcaat aatgtaaaat cgttaagcta ttggcctagc
      541 aataaatatt taaatgtttg ggttgtaaaa agcatacgcg gtgcacaacc acccttgcaa
      601 gttgccgggt ttgcacaatt tccgggtggt gcagcggcca cagatggtat agtaatttgc
      661 catcagtttt taggtaccat aggtacttca acaggcagtg gtgaaggccg cacaactaca
      721 catgaagttg gccactggct taacttaata catatttggg gtgatgatgc tggcgcatgt
      781 tcaggctctg attttgtaag cgatacgccc aatcaagcaa acctaaacca aagtaattgt
      841 cctacatttc cacataccga tgcatgtacg cctgcaggca atggtgtatt atatagcgat
      901 tacatggact acaccaatgg taattgccaa aatattttta cagttggcca atgtgcacgt
      961 atgaatgcag ccttatcttc tggtaccagt caacgcaaca acctatggtc gtcaaccaat
     1021 ttagtagcta ccggcaccaa tggaacaccg gcagtaccat gcgcacctat accttctgtt
     1081 gtttttggtg tacgaaatat ttgtgcaggt ggtaacttaa cttttaatga gtatgtatat
     1141 aatggtattc ctacatcgcg cgattgggtt tttaccggtg gcaaccctgc aaccagcacc
     1201 aatgccagcg aagtggtaac ttacaacaca cccggcacct ataatgttac ttatacagca
     1261 acaaatgcag taggctctaa cactactact tatgcaggca ttgtaaatgt gttgccaact
     1321 atcggtgtta aaagtgtgcc atatactgac ggatttgaag gcagtttccc cggttttgat
     1381 tacgatgtta ttaatggcga aggcaatttt acatgggagc aaaccagctt agctgccgca
     1441 acaggcacc
//
LOCUS       DAOMAA010000657         1449 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_105585_length_1449_cov_2.728121, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000657 DAOMAA010000000
VERSION     DAOMAA010000657.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105585_length_1449_cov_2.728121"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1157
                     /locus_tag="PLO59_08605"
     CDS             <1..1157
                     /locus_tag="PLO59_08605"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmpP1/FadL family transporter"
                     /protein_id="HQV01206.1"
                     /translation="GFQINTQSIKGVGMAGAYAGICKDASATFFNPGAMGFLDKSSLT
                     LGGSLLLPKASYLNPYGGNVDMESQTFFVPHLYGNYKLNEKISLGLSVNAPYGLGTKW
                     DDNWTGRYLSQEVHLQAIYIQPTASYNITENIGVGAGFVFTTGNATVRKASQAGNNDV
                     YANLEGGGIGFGFNVGVFGKLGDKTRVGISYRSKVKIDLEDGDATFTNVPASLLASGQ
                     LPASTKFNSGITLPGVLSVGASYQITDKWMATLQFDYTGWDVYDSLNFEFPDYTSLNS
                     NNARNYKNSSAFRLGIEYKATSKLTARIGAGFDQTPVQDDYVGPDLPDANKQLLTCGI
                     SYMLTEKFSIDASYMYENLDERNGTYKEENFSGSYKSRVHVFGVGLNYAF"
     gene            1195..>1449
                     /locus_tag="PLO59_08610"
     CDS             1195..>1449
                     /locus_tag="PLO59_08610"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014781595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HQV01207.1"
                     /translation="MKKITPYFLLLAALASSSCETSFDEPKSSAAPIDLTRYVALGNS
                     LTAGYADNALSLEGQQNAYPAILAQQFKKAGGAAVFNTPFL"
BASE COUNT          451 a          289 c          295 g          414 t
ORIGIN      
        1 gaggctttca aatcaataca caaagtatta aaggtgtagg tatggcaggc gcttatgccg
       61 gtatttgtaa agacgcctcg gctacttttt ttaatccggg cgcaatgggc tttttagata
      121 aaagttcatt aacactgggc ggctcattac tgctgcctaa agccagctac ctcaatcctt
      181 atggcggcaa tgtagatatg gaaagtcaaa ctttttttgt tccacattta tatggcaact
      241 acaagttaaa cgaaaaaata tccttaggcc ttagtgtaaa tgcaccttat ggcttaggta
      301 ccaagtggga tgataactgg accggacgct atctatcaca agaagtacat ttacaagcca
      361 tatatataca acctactgct tcctataaca ttaccgaaaa cattggtgtg ggtgcaggtt
      421 ttgtatttac aaccggtaac gctacggtac gtaaagcatc gcaagcaggt aacaatgatg
      481 tgtatgcaaa cctcgaaggc ggtggtatag gttttggatt taatgtaggt gtgtttggca
      541 aactgggcga caaaacgcgt gtaggcatta gctatagaag taaggttaaa attgatttag
      601 aagatggcga tgcaacattt accaatgtgc ctgcatcgtt attagcctca gggcaattgc
      661 cagcaagtac aaagtttaac tcaggcatta ccttacccgg tgttttaagt gtgggtgcca
      721 gctatcagat tactgataaa tggatggcca ctttacaatt tgattatacc ggttgggatg
      781 tgtatgattc gttaaacttt gagtttcctg attacacatc acttaactca aacaatgcac
      841 gtaattacaa gaacagttcc gctttccgtt tgggaatcga atacaaagcc acttccaaat
      901 taacagcacg tattggtgcc ggtttcgacc aaaccccagt gcaggatgat tatgtaggtc
      961 ccgatttacc cgatgccaac aaacagttat taacctgcgg tatcagttac atgctaacag
     1021 aaaaatttag catagatgcc agctatatgt atgaaaattt agatgaaaga aatggtactt
     1081 ataaagaaga aaacttttca ggctcctaca aatcgcgcgt acatgttttc ggtgtaggat
     1141 taaactatgc tttttaaacc caatgtattt caactcccat taaaaaaaca atcaatgaaa
     1201 aaaataactc cttacttttt actactggcc gcattagcca gcagcagttg cgaaacctct
     1261 tttgacgaac caaaaagtag cgctgcacca attgacttaa cacgctatgt ggctttaggc
     1321 aattcgctaa cggcaggtta tgccgataat gctttgagct tagaaggcca acaaaatgct
     1381 taccccgcta tattagcaca acaatttaaa aaagctggcg gtgctgccgt atttaataca
     1441 cctttttta
//
LOCUS       DAOMAA010000658         1449 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_105629_length_1449_cov_2.183644, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000658 DAOMAA010000000
VERSION     DAOMAA010000658.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105629_length_1449_cov_2.183644"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="PLO59_08615"
     CDS             <1..331
                     /locus_tag="PLO59_08615"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HQV01208.1"
                     /translation="GNNTNNQLPELDFEETRYHFGEVKQGEKINHTFKFTNTGNADLI
                     LATVSASCGCTVPEWNKEPIKPGEEGSIKVTFNTEGKTGMQSKTISVMSNAKPGTRVL
                     TISAEIN"
     gene            352..684
                     /gene="yajC"
                     /locus_tag="PLO59_08620"
     CDS             352..684
                     /gene="yajC"
                     /locus_tag="PLO59_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HQV01209.1"
                     /translation="MLSILLQADSGSPYSNLIMMASVVAIMYFFMIRPQMKKAKDEKK
                     FIESIEKGSKVVTLGGIHGKIVEMNDNTIMLEVDKNVTLKVEKKAISAEATKAYQQQT
                     TVVQADKK"
     gene            811..1068
                     /locus_tag="PLO59_08625"
     CDS             811..1068
                     /locus_tag="PLO59_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01210.1"
                     /translation="MWLLIALNNTYDTTLTYTLYANKTKRSIVVQVQGKGFELLKEKL
                     AHKRIQVQAKSISDTESYIRNNLPVNEHIKYLSFKPLSVSF"
     gene            1070..>1449
                     /gene="coaE"
                     /locus_tag="PLO59_08630"
     CDS             1070..>1449
                     /gene="coaE"
                     /locus_tag="PLO59_08630"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HQV01211.1"
                     /translation="MVRIGITGNIGSGKSTVALVFEALGIPVYFADKEAAALMTLPEI
                     KQKIIAATHNQIYNGDVLNKTALAEAMFNNDALRLQINAIVHPAVTQHFEIWFSQQHA
                     PYVLKEAALLFEAQSHALLDKIICV"
BASE COUNT          545 a          257 c          252 g          395 t
ORIGIN      
        1 aggtaataac acaaacaatc aactacctga attagacttt gaagaaaccc gttatcactt
       61 tggcgaagta aaacaaggcg aaaaaataaa tcacaccttt aagtttacca atacaggcaa
      121 cgctgattta atattagcaa ccgtatcggc atcgtgtgga tgtaccgtac ccgaatggaa
      181 taaagaaccc ataaaaccgg gcgaagaagg ttcgattaaa gttactttta ataccgaagg
      241 caaaacaggt atgcaaagta aaaccataag tgtaatgagt aatgctaaac ccggaacacg
      301 cgttttaacc atcagtgcag aaattaatta aaactaaaat atttcaaatt catgttatcc
      361 attttattac aagccgatag tggctcgcct tattcaaatt taattatgat ggcatccgtt
      421 gtagccatta tgtatttctt tatgataaga ccacaaatga aaaaagccaa agacgaaaaa
      481 aaatttattg aatcaattga aaaaggcagt aaagtagtaa ccttaggtgg catacatggt
      541 aagattgtag aaatgaacga caacaccatt atgcttgaag ttgataaaaa cgttaccctt
      601 aaagtagaaa agaaagctat ttcggccgaa gctacaaaag cttatcaaca acaaactact
      661 gttgtacaag ccgataaaaa ataattttaa aatcccattg ttctggctta ctacaaaaat
      721 acaaacaagg ccaccggcac acaggtaagt aaagcaaaaa ttaattatcg cattgtaata
      781 cttatttgga gctttgttgc tgcctgcact ttgtggttat taattgcatt aaacaatacg
      841 tatgatacaa cactaaccta tactttatac gcaaataaaa ccaaaagaag tatagtggtt
      901 caagtgcaag gaaaaggctt tgaattgtta aaagaaaaac ttgcccacaa acgtattcaa
      961 gtgcaagcaa agtctataag cgataccgaa agctatatac gtaataacct acccgttaac
     1021 gaacatatta aatatctttc gtttaaacca ctatccgtta gtttctgaaa tggttcgcat
     1081 cggtataaca ggcaatattg gtagtggcaa aagcacggta gcattggttt ttgaagcatt
     1141 aggcatacct gtttattttg ccgataaaga agcagcggca ttaatgacgt tgcctgaaat
     1201 caaacaaaaa ataattgccg caacccataa tcaaatttat aatggtgatg tgcttaataa
     1261 aacagcattg gcagaagcaa tgtttaataa tgatgcatta aggcttcaaa ttaatgccat
     1321 agtacaccct gctgtaacgc aacatttcga aatttggttt tcgcagcaac atgcacccta
     1381 tgttttaaaa gaggctgcct tattgtttga agcgcaaagc catgcgctat tagataaaat
     1441 tatttgtgt
//
LOCUS       DAOMAA010000659         1446 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_105981_length_1446_cov_3.212797, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000659 DAOMAA010000000
VERSION     DAOMAA010000659.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105981_length_1446_cov_3.212797"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            299..976
                     /locus_tag="PLO59_08635"
     CDS             299..976
                     /locus_tag="PLO59_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HQV01212.1"
                     /translation="MGIYVLMIVLMLAGWLVQMRLKSKFTKYSQTPLANGMSGKEIAE
                     RMLHDNNINDVRVISVEGQLTDHYNPADKTVNLSHDVYYGRNAAAAAVSAHECGHAVQ
                     HATAYSMLQLRSALVPIVSVSSRWTMWVLLAGVLLVNTFPSLLLVGIIMFAATTVFSF
                     ITLPVEFDASKRALVWLDTKGITTQAEHALSKDALWWAAMTYVVAALSSLATLMYYIM
                     IYMGRRD"
BASE COUNT          434 a          275 c          268 g          469 t
ORIGIN      
        1 gtaatttttg ccccaactca attatgtact gatatttttc ggcccaggtt tcgaaccact
       61 caaaatcggc aactattgct gcctcggcat tggccacact ttgcatgatt aaattttatt
      121 taaggccgca atttagtgta tataaatgcg tcaataaaat gcttttcaaa agtttagtct
      181 ttatcttcaa tttatactaa gaacgaagcc gtataaagta tgccattttg ccaatggtat
      241 aacttttgaa aaacgctgtt acctttgtac aattaaaata atttaaaaat tcaaaactat
      301 gggaatttat gtattgatga tcgtattgat gttggcgggg tggctggtac aaatgcgctt
      361 aaaaagcaag tttactaagt acagccaaac accattagcc aacggtatga gcggtaaaga
      421 aattgcagaa cgtatgttac acgacaataa tattaatgat gtacgtgtta ttagtgttga
      481 aggacaactt accgaccatt acaacccggc tgataaaact gtaaacctga gccatgatgt
      541 ttattatggc cgtaatgcag cagcagcagc cgtttcggct catgagtgtg gccatgccgt
      601 gcagcatgct actgcataca gtatgttgca attgaggtcg gcattggtgc ccattgtaag
      661 tgttagctcc aggtggacta tgtgggtatt attagccggt gtgctattgg taaatacttt
      721 tccaagtttg ttgcttgttg gtattatcat gtttgcggca acaacggtat tttcatttat
      781 aactttaccg gtagagtttg atgccagcaa acgtgccttg gtttggttag ataccaaagg
      841 tattaccaca caagctgagc atgcattatc aaaagatgca ttatggtggg cagctatgac
      901 ttatgttgtt gcagctcttt catcgcttgc aacgctcatg tactatatca tgatttatat
      961 gggcagacgc gattaatgtt tattttaacg gaactaaaaa aagcggtttg taaattttac
     1021 agaccgcttt ttttttcagg ttaataaagt cgttttttaa ttttttcaaa ccgttatcaa
     1081 gcaattagtt gttagtggtt agtggttagc ttttaccaat taacctttac cagacaacat
     1141 accactaacc actaacaact acccctttca actctcaact ccttttataa ctttaccacc
     1201 ataaaaccaa gtttccctta accctttaat tttatctgtt atgaaaaacg tagtaattat
     1261 taaaaaatca cacacacaca cacacaacaa aaatcagcag ttgtgcaagt aaaatcttat
     1321 ttattttaat gctaatggca atgcaactgc aaggccaaac gcctttatgg acacagcaat
     1381 acggcacatc cgcttacgat gccggctttt cggtttcaaa aacttttgac ggtggtttta
     1441 tagttg
//
LOCUS       DAOMAA010000660         1444 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_106314_length_1444_cov_2.318215, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000660 DAOMAA010000000
VERSION     DAOMAA010000660.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106314_length_1444_cov_2.318215"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="PLO59_08640"
     CDS             <1..307
                     /locus_tag="PLO59_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01213.1"
                     /translation="FISGDNDVIDTLQFVTPTKKMAGSIFSNLPKGLTANFSNNNQIT
                     FVDIKEFLQPMPKSLEEAKGLVTADYQTYLEKSWIEQLRKKYPVQVNDEVLKSIIKK"
     gene            382..1254
                     /locus_tag="PLO59_08645"
     CDS             382..1254
                     /locus_tag="PLO59_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01214.1"
                     /translation="MHHNNGYKYAIALFAFSILGCQYFKSKPTVDQTQAVARVYDKYL
                     FKNDLIKIIPKGASAADSMQAVRNYIDNWIKQNIILRKAENNLNDDEKNVQPQLDNYR
                     NTLITYIYQRELIKEKLDTTVTENEITEYYQTHKNNFALRQSIVRFIYLKTDKAAPKL
                     NQVRQWFKSNNNDDKIKLEDYCYQFATDYFLNDEDWVSIDQCLQKAGIITDNKDAYLQ
                     SNFYTEIPDSTQITFIRIKEFKYKDSISPIEFETDNIKNLIINKRKLGLIQEMEKAAY
                     QKALNEKDFEIYTQ"
     gene            1262..>1444
                     /locus_tag="PLO59_08650"
     CDS             1262..>1444
                     /locus_tag="PLO59_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013664759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQV01215.1"
                     /translation="MTTSKLKGYLIFLLCIYFGKAISQPQILDQVAAIVGSKIILQSE
                     VEQQYQVYLSQGNYAKP"
BASE COUNT          588 a          254 c          218 g          384 t
ORIGIN      
        1 atttattagt ggtgataatg acgtgataga taccttgcaa tttgttacac ccacaaaaaa
       61 aatggccggt agcattttta gcaacttacc taaaggtctt acagctaatt ttagtaacaa
      121 caaccaaatt acatttgtag atattaagga gtttttacaa cccatgccta aaagcttaga
      181 agaagcaaaa ggcttggtaa ctgccgatta tcaaacctat cttgaaaaaa gctggattga
      241 gcaacttcgt aaaaaatatc cggtacaggt aaatgacgaa gtgcttaaaa gcatcatcaa
      301 aaaataagca aattccgcta aactattata caaagtgaac ctgaaaaatc aggttcactt
      361 tgttatttaa agtagtaaaa catgcatcac aataatgggt ataaatatgc aattgcattg
      421 tttgcttttt caatattggg ctgccaatat tttaaatcaa aacccactgt ggatcaaaca
      481 caggctgttg cccgtgtcta tgataaatat ctttttaaaa acgacttaat aaaaataata
      541 cccaaaggag catcggctgc cgacagcatg caagccgtta gaaattacat tgataattgg
      601 attaaacaaa acattatact tcgtaaagcc gaaaacaatt tgaacgatga cgaaaaaaat
      661 gtacaaccgc agttagataa ctatcgaaat acactcataa cctatatata ccaacgcgag
      721 cttattaaag aaaagttaga tacaaccgta accgaaaacg aaataacaga atactatcaa
      781 acacataaaa ataactttgc cttgcgtcaa agcattgtta ggtttatcta cttaaaaacc
      841 gataaagcag cacctaaatt aaaccaggta aggcaatggt ttaaatcaaa caataatgat
      901 gacaaaataa aattagaaga ctattgttat cagtttgcaa ccgactattt tttaaatgat
      961 gaagactggg tatctatcga ccaatgcctt caaaaagcag gtattataac cgataacaaa
     1021 gatgcatact tacaaagtaa tttttatacc gaaatacctg atagtacgca aattacgttt
     1081 atacgaataa aagaattcaa atataaagac agtatttcgc ccattgaatt tgaaaccgac
     1141 aacattaaaa atctgataat aaacaaacgt aaattagggc ttattcaaga aatggaaaag
     1201 gccgcttatc aaaaagcact caacgaaaaa gactttgaaa tttacacaca ataacccata
     1261 aatgactaca tcaaaattaa aaggctactt aatttttctt ttgtgtattt actttggcaa
     1321 agcaataagc caaccgcaaa tattagatca ggtagcagcc atagtaggca gtaaaattat
     1381 tttgcaaagc gaagtagaac aacaatacca agtgtactta tcacaaggaa actacgccaa
     1441 accc
//
LOCUS       DAOMAA010000661         1443 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_106380_length_1443_cov_3.890490, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000661 DAOMAA010000000
VERSION     DAOMAA010000661.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106380_length_1443_cov_3.890490"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..>1443)
                     /gene="gldG"
                     /locus_tag="PLO59_08655"
     CDS             complement(138..>1443)
                     /gene="gldG"
                     /locus_tag="PLO59_08655"
                     /inference="COORDINATES: protein motif:HMM:TIGR03521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HQV01216.1"
                     /translation="NDESSQKIIFPGAIVTYNSLELPVQLLKDQFGTPPEEMLNNSIR
                     NLEYELASVIKKATTKLPPQIAFLEGQNELNGPALSDIKKTLGKFYGLDSIRIDEKLD
                     VLKRIQLLIVAKPDTAFSEKDKFIIDQYVMNGGKILWCMDATNASMDSLARLPEAMAL
                     SKTLNIDDMLFRYGVRINADLIMDMQAVPIPVMTRNYMGNQPVQKMLPWPFFPMLFST
                     SSHPIVENLNAIKTEFASSMDTINNSGVVKTVLLTSSKYSRAAQVPVRISLDIFDSKP
                     NPKFYNKSYLPVAVLLEGTFTSLYKNRIPAALASDTLIKYKEQSVPNKMIVISDGDIL
                     KNTTSKNGQVFPLEYDRYTNQFFGNKNLLLNMVDYLTDDSGLISLRSKQIKLRLLDKN
                     YIQANTFNIQAINVVLPLLLILFLGITKIMFRKKQYARIAKK"
BASE COUNT          434 a          244 c          263 g          502 t
ORIGIN      
        1 ttgcaaagtc actgttttgt tgattgatgg ttgttttttt attgtttacg ataagccaaa
       61 ctgcaactgc agcaagcaaa ataaaaacta ccaaaagcca tttgtttttt ttcatgaaat
      121 gttataagta aatgctgtta tttttttgca atacgggcat actgtttttt gcgaaacatt
      181 attttggtta tacccaaaaa taaaattaac aacagcggta ataccacatt tattgcctgt
      241 atattaaaag tattggcctg gatgtaattt ttatctaaca ggcgtaattt aatttgttta
      301 ctgcgtaatg aaataagtcc actatcatca gttaaataat caaccatgtt aagcagcaaa
      361 tttttatttc caaaaaactg attggtataa cgatcgtact caagtggaaa tacctggccg
      421 ttttttgaag tagtattttt aagtatatcg ccatcactaa ttactatcat tttattgggc
      481 acactttgtt ctttgtactt tattaaagta tcggatgcta acgctgccgg tatgcggttt
      541 ttatacagtg atgtaaaagt accttcaagt aaaacagcta caggcaggta ggatttatta
      601 taaaacttgg ggttgggttt cgagtcaaaa atatctaaac ttatccgaac cggaacttgg
      661 gcagcacggc tgtatttgct cgaagtaagt aaaactgttt ttacaacacc actattattt
      721 attgtatcca ttgaacttgc aaactcggtt ttaatggcat ttaaattttc gacaatggga
      781 tgtgatgagg tgctgaaaag catgggaaaa aatggccagg gtaacatttt ttgaaccggc
      841 tgattgccca tataatttcg tgtcattaca ggtatgggta cagcttgcat atccattatc
      901 aaatcggcat taatgcgcac accgtaacgg aataacatat catcaatatt aagtgtttta
      961 cttaatgcca ttgcttcggg taagcgtgca aggctatcca tactggcatt ggtggcatcc
     1021 atacaccaaa gtattttgcc gccattcatc acgtattgat caataataaa tttatctttt
     1081 tcgctaaatg cagtgtcggg cttggcaact attaaaagtt gtatgcgttt tagtacatcc
     1141 aatttttcat caattctaat cgaatcaagt ccataaaatt tgcccagcgt tttttttata
     1201 tccgataggg cagggccatt caattcattt tgtccttcca aaaatgcaat ttgtggcggt
     1261 agtttggttg ttgctttttt tattactgaa gccagctcat actctaaatt acgaatggaa
     1321 ttattaagca tttcttcggg gggtgtgcca aactgatctt ttaataattg aaccggtaac
     1381 tccaagctgt tgtaggtaac aatagcaccc ggaaaaatta ttttttgtga tgattcatca
     1441 ttg
//
LOCUS       DAOMAA010000662         1441 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_106760_length_1441_cov_2.128427, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000662 DAOMAA010000000
VERSION     DAOMAA010000662.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106760_length_1441_cov_2.128427"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..>1441
                     /locus_tag="PLO59_08660"
     CDS             105..>1441
                     /locus_tag="PLO59_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01217.1"
                     /translation="MIKLITANAGLLILIFFTQCRKENFTTDASAKLDFSSDTVLFDT
                     VFVTMGSTNKQFTIHNRNNDALKISNIRLSGGAASVYRLNVDGLPGINFNDVEIPAND
                     SLWVFVEVTINPNTTNLPFIVADSIVFETNGNLQKVQLVAWGQNAHFFKPTNSQPGLP
                     DYSIIPCNAVWDSVLPYVIYGYAVVDSGCSLTIKEGTKIYFYNSSGLWVFSGGNLQVT
                     GTLAHPVVFQGSRLEANYQNTPNQWDRIWINEGGSNQIDYAIIKNGFIGLQLEELFEP
                     GLPKNIIVNNTVIKNMNGVGVLTRGYNAVFNNCEISNCGSYLAALTQGGNYAFNHCTM
                     ANFWRFGKRSTPSVYLNNYLVNQNNMPVGVNLDKADFKNCILYGNEENEFETDFLTGS
                     AANFLFDHSILKVKASTDVTNPLHYNNIYKNNNPNFKNTNEGDFTLDTLAFAQ"
BASE COUNT          457 a          241 c          261 g          482 t
ORIGIN      
        1 ttaattttca aactttatca ttaaaattta cgttgttgtt tttacgtcaa aactatttgt
       61 aacatatttt accatttgtg taaataaact acgtttgctg cttcatgata aagctaatta
      121 ctgccaatgc cggtttactt attttaatat tttttacgca gtgccgtaaa gaaaatttta
      181 caaccgatgc ctcggccaag cttgatttta gtagcgatac tgtattgttt gataccgttt
      241 ttgtaacaat gggttcaaca aacaagcagt ttaccataca taaccgtaat aacgatgcat
      301 taaaaattag taacatcagg ttatcgggtg gtgcggcatc agtttatcgg cttaatgtag
      361 atggcttacc gggcattaat tttaatgatg tcgaaattcc ggcaaacgac tcgttatggg
      421 tttttgttga agtaacaatt aatccaaaca caaccaattt gccatttatt gttgccgact
      481 caattgtatt tgaaaccaat ggaaatttgc aaaaagtaca attggtagcc tggggtcaaa
      541 atgcacattt ttttaagcct accaactcgc aacctggttt acccgattac agcattatac
      601 cctgcaatgc cgtttgggat agtgttttgc cttatgttat ttatggctat gctgttgttg
      661 attcgggctg ttcgctaact attaaagagg gcactaaaat ttatttttat aacagttcag
      721 gcctttgggt ttttagtggt ggtaatttgc aggttacagg tacattggcg catcctgtag
      781 tttttcaagg cagccggtta gaagcaaact atcaaaacac tcctaatcaa tgggacagga
      841 tatggattaa cgaaggtggt tcaaatcaaa tagattatgc cataattaaa aatggtttta
      901 ttggattgca acttgaagag ttatttgaac cgggcttacc caaaaatatt attgtaaaca
      961 atacggttat taaaaacatg aatggtgtag gtgtacttac acgtggctac aacgctgtat
     1021 ttaataattg tgaaattagt aattgcggca gttacttagc tgctttaaca caaggtggca
     1081 actatgcttt taaccattgc accatggcta acttttggcg ctttggtaaa cgcagcacac
     1141 ccagtgtgta tttaaataat tatttggtta atcaaaataa tatgcctgtt ggtgttaatt
     1201 tagataaagc cgattttaaa aactgtatat tatatggtaa tgaagaaaac gaatttgaaa
     1261 ccgatttttt aaccggcagc gcagccaact ttttatttga tcatagcata ttaaaagtta
     1321 aagcaagtac ggatgttacc aaccctttac attacaacaa tatttacaaa aacaacaacc
     1381 ctaattttaa aaataccaat gaaggcgatt ttactttaga caccttggca tttgcacaaa
     1441 a
//
LOCUS       DAOMAA010000663         1437 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_107370_length_1437_cov_1.746744, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000663 DAOMAA010000000
VERSION     DAOMAA010000663.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107370_length_1437_cov_1.746744"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1140)
                     /locus_tag="PLO59_08665"
     CDS             complement(<1..1140)
                     /locus_tag="PLO59_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020080623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HQV01218.1"
                     /translation="MTLFAYLNTQAQFIDSETQSRLKQHINTLASNKLEGRETGTIGE
                     KLAATYITKTFTELGLKADGDSGYYQAFPFFNNYKPANSRFSVGQKTFNINDDFYAYP
                     SKLDAQVVGRITDVGYGLNAPALNYNSFDGLTNLKGTLFLIQIGHPENGNPHSRFESM
                     SDIQSKLDTAMAYGAIGVIFICGNESFKKQKPDYAKRIGKETIPAIIINKDCARYIKN
                     NLFEKASITTSIKKEMGIGYNLIGSINNNKPYTVVLTAHYDHLGYGDEGSLHTGKKAI
                     HNGADDNASGVAAILELARLLQKSSFKNYNYTFIAFSGEEKGLLGSNYYVKQLGTPLA
                     SFNYNINLDMVGRLSTTNPVLQINGVGTAMEWPAEIEKLTDLKIST"
BASE COUNT          437 a          267 c          253 g          480 t
ORIGIN      
        1 agtgcttatc tttaaatctg taagtttctc aatttcagcg ggccactcca ttgcagtgcc
       61 cacaccattt atttgtaaaa cggggttggt agtgctaagc ctgccaacca tatctaaatt
      121 tatattgtaa ttaaacgaag ctaatggtgt gccgagttgc ttcacataat aattgctgcc
      181 caacaatcct ttttcttcac cactaaaagc aataaaagtg tagttgtaat ttttgaagct
      241 acttttttgt aacaaacgcg ccaactctaa aattgcagct accccacttg cattgtcgtc
      301 agcaccatta tgaattgctt ttttaccggt atgtaatgag ccttcatcgc cataacccaa
      361 gtggtcgtaa tgtgctgtaa gcacaactgt atagggtttg ttgttgttaa tgctgccaat
      421 taggttataa ccaataccca tttccttttt aatagaagtt gtaatgctgg ccttttcaaa
      481 caggttgttt ttaatatatc gggcacaatc tttatttatg ataattgcgg gtatggtttc
      541 tttaccgata cgtttggcat aatcaggttt ttgtttttta aacgattcgt tgccacaaat
      601 aaatataacg ccaatggcac cataagccat tgcagtatcc aacttacttt gtatatcaga
      661 cattgactca aaacggctat gcggattgcc gttttcggga tgcccaattt gtattaaaaa
      721 cagcgttcct tttaaattag taaggccatc aaagctgttg taatttaatg caggcgcatt
      781 taaaccatat ccaacatcag ttatacgacc aacaacctgc gcatcaagct tcgaagggta
      841 ggcataaaaa tcatcattaa tattaaacgt tttttgacct acactaaagc ggctgtttgc
      901 tggcttataa ttattaaaaa acggaaaagc ctgatagtac cccgaatcac catcagcttt
      961 taagcccaat tcagtaaaag ttttggttat atatgttgcc gctaattttt cgcctatagt
     1021 tccggtttct cggccctcca acttattgga ggctaaagta ttaatatgtt gttttaagcg
     1081 actttgcgtt tcgctatcaa taaactgtgc ttgcgtatta aggtaagcaa aaagtgtcaa
     1141 aaagtaaaag aatggtttgt tcattaaaag aagatggtgt tacaaaaaat aaaatttgat
     1201 gcgcaaaact atttacggat atcttattcc caaaagttag actataaatt tcaaacaagt
     1261 tgtatacgaa tgctgtttga ttaccaaact gtcaaataat gaccggtacc tgcgtcttgg
     1321 attgtaacaa cacgttgata tatcgggtaa attttatgct gtttttgtat gaggcagcta
     1381 ctttgtgttt aagcttattt aaactacgtt tgcccgatat ttaagaaaaa aataatg
//
LOCUS       DAOMAA010000664         1435 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_107582_length_1435_cov_2.863768, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000664 DAOMAA010000000
VERSION     DAOMAA010000664.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107582_length_1435_cov_2.863768"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="PLO59_08670"
     CDS             <1..823
                     /locus_tag="PLO59_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011450022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQV01219.1"
                     /translation="AHVEKAMAGCDYVFHLAAYAAEGLSHFIKRFNYQNNLIGSVNLI
                     NESVKHHVKCFVFTLSIAVYGEGQLPMRENMTPIPEDPYGNAKLAVENELRITHDMFN
                     LNYVIFRPHNVYGENQNLGDRYRNVIGIFMNQLMQNLPLTVYGDGEQTRAFSYIDDVA
                     PHIANCVKIPAAQNQIFNIGADKHCTVNELAHYVMDAMQQKGTINYLPHRNEVMHVFS
                     DHTKARKVFELTDPITLEAGLASMAAWAKKNGSHKSKPFKHIEIYKKLPPHWLEQ"
     gene            complement(833..>1435)
                     /locus_tag="PLO59_08675"
     CDS             complement(833..>1435)
                     /locus_tag="PLO59_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01220.1"
                     /translation="NVVYLANTSILYCKMGLRQVSDAQKQTYYHKGAYLALKAIKANS
                     NNADAHYAYAFALGRINEFASSKQKIANAKTIKTECDKTLSIDANYAGAYHIMGRWHR
                     TIAGFSSVERFMINSFFGGVPPGGTYEDAIKSFSKAITLEPKYILHQYELAMTYYERD
                     AQNDMAQVKSWCERALKCPINNVDDEGTKKKTMELLAKVK"
BASE COUNT          426 a          279 c          272 g          458 t
ORIGIN      
        1 ggcgcatgtt gaaaaggcaa tggcaggctg tgattatgtt tttcatttgg ctgcttatgc
       61 tgccgaaggc ttaagtcatt tcatcaaacg ctttaattat cagaacaatt taataggcag
      121 tgtaaatttg attaacgaat cggttaaaca ccacgttaaa tgttttgtgt ttacattatc
      181 tattgctgtg tatggcgaag ggcaattacc catgcgcgaa aatatgacgc ccattcccga
      241 agacccttat ggaaatgcca aacttgctgt tgaaaatgaa ttgcgtatta cgcatgacat
      301 gtttaattta aactacgtta tttttaggcc gcacaatgtg tatggcgaaa atcaaaactt
      361 aggcgatcgc tatcgcaatg ttattggcat ttttatgaat caattaatgc aaaacctgcc
      421 acttactgtt tatggcgatg gtgagcaaac ccgtgctttt agttacattg acgatgttgc
      481 gccacatatt gctaactgtg taaaaatacc tgccgctcaa aatcagattt ttaatattgg
      541 tgctgataag cattgtaccg taaatgaatt ggcacattat gtaatggatg ccatgcagca
      601 gaaagggacc atcaattatt tacctcacag aaatgaggta atgcatgttt tttctgacca
      661 taccaaagca cgcaaggttt ttgaattaac cgacccaatt acattagaag ccggtttagc
      721 aagtatggct gcatgggcaa aaaaaaatgg ttcgcataaa agcaaaccat ttaaacatat
      781 cgaaatatat aagaaactgc caccgcattg gttagagcaa tagtatttgt acttatttta
      841 ctttggccaa caactccatt gtttttttct tagtgccctc gtcatcaaca ttatttatcg
      901 ggcattttag tgcacgctca caccacgact taacctgagc catatcattt tgcgcatcgc
      961 gttcataata agtcattgcc aattcgtatt gatgtaaaat gtatttgggt tcaagtgtaa
     1021 tggctttact aaaactttta atagcatctt catacgtacc gcccggaggc acaccgccaa
     1081 aaaatgaatt tatcataaaa cgctctacac tgctaaaacc tgcaatggtg cggtgccagc
     1141 gtcccataat gtgataggca ccggcataat ttgcatcaat acttaaagtc ttatcacatt
     1201 cggtttttat agtttttgca ttggcaattt tttgtttgct gcttgcaaat tcattaatgc
     1261 ggcctaaggc aaatgcataa gcataatgtg catcggcatt gttgctattt gctttaatgg
     1321 cttttaatgc caggtacgca cctttatggt agtaggtttg tttttgcgca tccgatactt
     1381 gtcgtaaacc cattttacaa tacaaaatac tggtatttgc caggtaaacc acatt
//
LOCUS       DAOMAA010000665         1435 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_107658_length_1435_cov_1.788406, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000665 DAOMAA010000000
VERSION     DAOMAA010000665.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107658_length_1435_cov_1.788406"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..>1435
                     /locus_tag="PLO59_08680"
     CDS             173..>1435
                     /locus_tag="PLO59_08680"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HQV01221.1"
                     /translation="MTKFFILLFAAIFCLNTSAQRNVILIIADDLGTDYLSFYEGSLD
                     TANMPRLRHLAETGLRFKQAIANPVCSPTRAGLLTGRFSFRNGIGNFIGANNGEPELD
                     TAEILIPRVLNLIQPNAVKSAVFGKWHLHRPTSSSNLLIPQYMGFNTCVGSFEGSLNN
                     FYNWTKYTDSIQTTCTNYATTQTVNDALNWAKLQGDTSFFICMSFNAPHTPYHNPPAN
                     LHTVPGCIGTKTDINTYPQKYFKAALEAMDTEIGRLLDSLSFWNKLDSTYIIFTSDNG
                     NFKKVAQNVNAQRSKSTIYQAGIHVPLIINGPGVLQTPRTSNALVNLQDLFATIIEMF
                     GFDNWQPYFPQNTITDSHSLLPILRNNTDSVRPFAFAEIFRLNPDSMNGKTIRNSKYK
                     LLLFDDGRKELYNLLTDSNELNNLLLSAL"
BASE COUNT          472 a          268 c          247 g          448 t
ORIGIN      
        1 ataatccaat cattcaaaat aaattagaaa tcattaaaac aaaaactaaa ctaaaacaac
       61 catacaattt ttaagtaatt tgataatttg taacttcgtt aaataaataa agccccaatt
      121 ttgctattgc cctgtttata accaccttgg cttagtataa cattttaaat gaatgactaa
      181 gttttttatc ctattgtttg ctgcaatatt ttgccttaat acaagtgcgc agcgaaatgt
      241 aattttaatt attgctgatg atttaggtac tgattactta agcttttatg agggtagttt
      301 agataccgct aacatgccca gattaaggca cttggccgaa acaggcttgc ggtttaagca
      361 agcaattgca aatccggttt gttcgcctac gcgtgcaggc ttgttaaccg gacgatttag
      421 tttcagaaat ggaattggca attttattgg cgcaaataat ggcgagccag agttagatac
      481 cgctgagatt ttaattccac gcgtattaaa cctaatacaa ccgaacgctg ttaagtcggc
      541 agtttttggt aaatggcatt tgcacaggcc aacatcatca tcaaacttgc tcattccgca
      601 atacatgggt tttaatacct gtgttggtag tttcgaaggc tctttaaata acttttataa
      661 ctggacaaaa tataccgata gcatacaaac aacttgtacc aattatgcaa ctacacaaac
      721 tgtaaatgat gcactaaact gggctaagct gcaaggcgac acctcatttt ttatttgtat
      781 gtcgtttaat gcaccgcata caccttacca taacccacct gccaatttgc ataccgtacc
      841 cggttgtatt ggaacaaaaa cagacattaa cacctatcct cagaaatatt ttaaagcagc
      901 attagaagcc atggatactg aaataggtcg tttgcttgat tcattatcat tttggaataa
      961 attggatagt acctatatta tatttacaag tgataacggt aattttaaaa aggtagcgca
     1021 aaatgtaaat gcccagcgat caaaaagtac catatatcag gccggtatcc atgtgccgct
     1081 aattattaat ggccctggag tactacaaac accacgtacc agtaatgcat tggttaattt
     1141 gcaagactta tttgcaacaa taattgaaat gtttgggttt gataactggc aaccctattt
     1201 tccacaaaac actataactg attcgcatag cttattgccc attctgcgaa acaatacaga
     1261 tagtgtgcgt ccgtttgcat ttgccgaaat tttcagactt aatcccgatt cgatgaatgg
     1321 aaaaaccatc aggaatagta agtataaatt actactattt gatgatggcc gtaaggagtt
     1381 gtacaatctt ttaactgaca gtaatgagtt gaacaattta ttgttatcgg cttta
//
LOCUS       DAOMAA010000666         1434 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_107714_length_1434_cov_3.108774, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000666 DAOMAA010000000
VERSION     DAOMAA010000666.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107714_length_1434_cov_3.108774"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1290)
                     /locus_tag="PLO59_08685"
     CDS             complement(<1..1290)
                     /locus_tag="PLO59_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015029213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01222.1"
                     /translation="MHTAPLHSFHIPVMGLGFTIDTPIKVARYGISSVISIIEDELVE
                     QMRKFHSKEAGEIFVPVSNTEDDFRAKRFTAYLNLINRMVSKQMEIIRKLPFEPGNDL
                     SKYFELLPDNALLRKLYRQMMDENDVTKKHAMQQQLRNQIQAGAIDANIMSKVDKTNF
                     NKSDEALANEYSDALASLRGFANSDLHASVIFSAGYNPRLYAYIESFPDFYPDATGYL
                     KKKIVLKVSDYRSAIIQGKILAKKGIWISEFRIESGLNCGGHAFATDGILLGPILEEF
                     KEKRNTIANELFDLCNQALAIKGYNTFKAMPQLRITVQGGIGTAAENKFLFEHYQIDG
                     TGWGSPFLLVPEATNVDADTLQQLAQARKEDYYMSYASPLGIPFNNFRKSTSEQQRLK
                     RIQKGRPGSPCYKKFLVTNTEFTERPICTASRQYQHLK"
BASE COUNT          419 a          292 c          254 g          469 t
ORIGIN      
        1 ttttaaatgt tgatattgac gcgaagccgt acatatgggt cgttcggtaa actctgtgtt
       61 ggtaactaaa aattttttgt aacaagggct gcccggtctt cctttttgga tgcgttttaa
      121 acgttgttgt tccgaagtac tttttctgaa attattaaac ggaataccta aaggcgatgc
      181 ataactcatg taataatctt ccttgcgcgc ctgtgccaac tgctgcagtg tatcggcatc
      241 aacattggta gcttcgggta ctaatagaaa cggactaccc catcctgtgc catcaatctg
      301 ataatgctca aataaaaact tgttttcagc tgctgtacct attccaccct gcaccgttat
      361 acgaagttgc ggcattgctt taaatgtatt gtaacctttt attgcaagtg cctgattgca
      421 taaatcaaat aactcgtttg caatggtgtt tcgtttttct ttaaactctt ccaatatggg
      481 acctagtaaa ataccatcgg ttgcaaatgc atggcctcca caatttagtc ccgattcaat
      541 tcggaactcc gaaatccaaa tacctttttt agctaaaatc tttccttgaa taatagccga
      601 acggtagtcg cttactttta aaacaatttt ctttttcaga taaccggttg catcgggata
      661 aaaatccgga aacgattcga tgtatgcata taaacgcgga ttgtatcctg ccgaaaaaat
      721 taccgaagca tgcaaatcgc tattggcaaa accacgtaac gatgccaatg catctgaata
      781 ctcgttggcc aacgcttcat cgcttttatt aaagttggtt ttatcaacct tcgacataat
      841 attggcatca atggcaccgg cttgtatttg gtttcgcagt tgttgttgca tagcatgttt
      901 tttggtcaca tcattttcat ccatcatttg acgatacagc ttgcgcaata gtgcgttatc
      961 aggtaataat tcaaaatact ttgaaaggtc gtttccgggt tcaaatggca gcttgcgtat
     1021 aatttccatt tgtttactta ccatgcgatt aatcagattt aaatatgctg taaatcgttt
     1081 ggctctgaag tcgtcttcgg tattagaaac cggcacaaaa atttcgcccg cctctttact
     1141 gtgaaacttg cgcatctgtt ctaccaattc atcctcaata attgaaataa ccgatgaaat
     1201 accataacgt gctactttta taggtgtgtc aatagtaaag cctaaaccca taacaggtat
     1261 gtggaatgaa tgtaaaggtg ccgtgtgcat aatatcaatc gtttgagttt atatactaaa
     1321 taatgcagca aagcactcca tttattaagc tacaaaacat gatttaaatc actttaaaca
     1381 atgcagtacg ccacttaaca atacattata tcatggcatt gtaaaatacc tttt
//
LOCUS       DAOMAA010000667         1434 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_107751_length_1434_cov_2.585932, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000667 DAOMAA010000000
VERSION     DAOMAA010000667.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107751_length_1434_cov_2.585932"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1434)
                     /locus_tag="PLO59_08690"
     CDS             complement(<1..>1434)
                     /locus_tag="PLO59_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008507685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HQV01223.1"
                     /translation="KESKESLFDFINKNFVLFFKDLSFTLQSVNEVTTLVNEYISNNN
                     NPAEGMLQPVIADDFENENTLQQHWQHFTNIEFGGKSYYTNPTTFTFNQQPQPAFNKN
                     FNLLFDNLYANQNKKYENVILADNPKQIERLYAIMEDIEKPAEAKSKPLQFTTQLLAL
                     HQGFVDDDLKFVCYTDHQIFNRYHRFRLKKSFEKDQAISLKEIYALKPGDFITHIDHG
                     VGRFGGLETVIVNGKMQEAIRLLYKDNDLLYISIHSLHRISRYSGKDGTEPSLNKLGS
                     QAWANLKSKTKKKVKDIAKDLIALYAKRKAQKGFGFSPDNFLQTELEASFIYEDTPDQ
                     IKATVDVKQDMQKPQPMDRLICGDVGFGKTEIAIRAAFKAVCDSKQVAVLVPTTILAS
                     QHYRTFKERLKEFPCNIDYINRFKTTAQQKETLQKLSSGKIDILIGTHRLLSKDVVFQ
                     NLGLMIIDEEQKFGVASKEKLKAMKVNV"
BASE COUNT          394 a          252 c          268 g          520 t
ORIGIN      
        1 cacattaact ttcattgcct ttaatttttc ttttgatgct acaccaaatt tttgttcttc
       61 atcaattatc attaaaccta agttttggaa cactacatcc ttacttaata acctgtgtgt
      121 accaattaaa atatcaattt tgccgctgct taatttttgt aaggtttctt tttgttgggc
      181 tgtagtttta aaccggttta tgtaatcaat attacatgga aactctttca atcgttcttt
      241 aaaggttcga taatgttgtg atgctaaaat ggtagtaggt actaaaacgg caacttgctt
      301 actgtcgcaa acggccttaa aagcagcacg tatggcaatt tcggttttgc caaaaccaac
      361 atcaccacaa attaagcgat ccattggctg tggcttttgc atatcctgtt ttacatcaac
      421 tgttgcttta atctggtcgg gggtgtcttc gtaaataaag gaagcttcta actcggtttg
      481 caaaaagtta tcaggcgaaa aaccaaagcc tttttgtgct ttacgttttg cataaagtgc
      541 aatcaggtct tttgcaatat cttttacttt cttttttgtt tttgatttga gattggccca
      601 tgcctgtgag cctaatttat ttaacgaagg ttcggtgcca tccttacccg aatagcgtga
      661 tatgcggtgt aggctgtgga tactgatgta aagcaagtcg ttatccttat ataataaacg
      721 aatggcttct tgcattttac cgtttacgat aacggtttct aaaccaccaa agcgacccac
      781 accgtggtca atatgtgtta taaaatcacc gggtttaagc gcataaattt ctttaagcga
      841 tatggcctga tctttttcga agcttttctt taatctgaac cggtgatagc ggttaaaaat
      901 ctgatgatcg gtataacaaa caaattttaa atcatcgtca acaaagcctt ggtgtagtgc
      961 caatagctgc gtggtaaatt gcagtggctt tgattttgcc tcagccggtt tttcaatatc
     1021 ttccattatg gcatacaggc gttcaatttg tttggggtta tcggccagta ttacgttttc
     1081 atatttcttg ttttgattgg cgtacaggtt atcaaacaac agattgaaat ttttattaaa
     1141 cgcaggttgt ggttgctgat taaatgtaaa agttgttggg tttgtataat atgacttgcc
     1201 tccaaattcg atgttggtaa aatgttgcca atgttgctgc aaggtgtttt cgttttcaaa
     1261 atcatcagct attaccggct gcaacatacc ttcggcaggg ttgttattat tgctgatata
     1321 ttcgtttacg agtgtagtta cctcattaac agattgtagt gtaaaggata agtctttaaa
     1381 aaaaagcaca aagtttttat ttataaaatc aaacaatgac tctttacttt cttt
//
LOCUS       DAOMAA010000668         1433 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_107857_length_1433_cov_3.525399, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000668 DAOMAA010000000
VERSION     DAOMAA010000668.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107857_length_1433_cov_3.525399"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1433)
                     /locus_tag="PLO59_08695"
     CDS             complement(<1..>1433)
                     /locus_tag="PLO59_08695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01224.1"
                     /translation="GSASGAAYINLHPNPAITSNTFFSNPQSPLSTTDYTISKYKVHL
                     KNGPTITHYRQTRDSMLNITGIAPGMYDLLIYVGDSMTPNSPGFGLDSTLIYTGTLQI
                     TDDTLCSLVKGRVFADYNSNCSYEATDLAIPNTIIELNPGGMVGVTDATGNFFIPVTP
                     GSYTLKQYAPAAFKQVCPDTNTFVINIGTGNIVNVPVADTITAQPDVYISCAAGAARP
                     GFSQNIFIRYANNTPHNIGVGQGQFQYDTLQTFVSFTGGTYTTTTGLVSFNLPALSAF
                     GKADFVLVLHTPATAVLGDTLSYMANVPVVLNEVDSLNNSSQQIRIITGSYDPNDITV
                     TPVGFGSAGYVTAYQDLNYQIRFQNTGTDTAFNIVVADTLAMELNRLSVQVLQASHPY
                     RLEIKGRVLQFHFDNILLPDSGTNHIASNGFIVYRIMQAANNAPGTTIKNTAHIYFDF
                     NAPITTNTVTNTIFDCASMASMSINQT"
BASE COUNT          436 a          267 c          295 g          435 t
ORIGIN      
        1 tgtgtttgat tgatagacat tgatgccatg gaagcacaat cgaaaatggt gtttgtaact
       61 gtattggtag ttataggtgc attaaagtcg aaataaatat gtgctgtgtt tttaatagtg
      121 gtacccggtg cattattagc tgcttgcata atacgataca ctataaaacc attgcttgct
      181 atatggtttg tgccgctatc gggtagtaaa atattatcga aatgaaattg cagaacccgg
      241 cccttaattt ctaaacgata tggatgtgac gcttgtaaaa cctgtaccga caatctgttt
      301 aactccattg ctaaagtatc ggccactaca atattaaaag cagtatcggt acccgtattc
      361 tgaaaccgga tttgataatt taaatcttga taggctgtta cataccctgc cgaaccaaac
      421 cctactggtg ttacagttat atcatttggg tcataggagc cagtaataat tctaatttgc
      481 tggctgctgt tgtttaatga gtcaacttcg ttaagcacta ccggtacatt ggccatataa
      541 cttaacgtat cgcccagcac agccgtagca ggtgtgtgaa gtactaatac aaaatcggct
      601 ttgccaaagg cagataaagc aggcaaattg aaacttacta aacctgttgt tgtagtataa
      661 gttccgcctg taaagcttac aaaagtttgc agcgtatcat attgaaactg cccctgacct
      721 acgccaatat tatgaggcgt attgtttgca tatctgataa atatattttg actaaatccc
      781 ggacgggctg caccggcagc gcaacttata tacacatcag gctgtgctgt aattgtatcg
      841 gctaccggta catttacaat attacccgta ccaatattta ttacaaaagt attggtatcg
      901 gggcatactt gtttaaatgc agcaggcgca tattgcttta aggtataact gcccggtgtt
      961 acaggtataa agaaattacc ggtggcatcg gtaacaccta ccataccacc ggggtttaat
     1021 tcaataatgg tatttggtat cgctaaatca gttgcttcat aactacagtt actattataa
     1081 tccgcaaaaa cacgaccttt aaccaaacta cataaggtat catcggtaat ttgtaaggta
     1141 ccggtataaa ttaaagtaga gtctaatcca aagcccgggc tatttggagt cattgaatcg
     1201 ccaacataaa tcaataaatc atacataccc ggtgcaatgc ccgttatatt tagcatggag
     1261 tcgcgcgttt ggcggtagtg tgtaatagtt ggaccgtttt ttaaatgaac tttatattta
     1321 gaaattgtat agtctgttgt acttaaaggc gattgtggat tactgaagaa ggtattagat
     1381 gtaatagcag gatttggatg caggttaatg taagcagcac ccgatgccga acc
//
LOCUS       DAOMAA010000669         1433 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_107884_length_1433_cov_2.727141, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000669 DAOMAA010000000
VERSION     DAOMAA010000669.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107884_length_1433_cov_2.727141"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1078
                     /locus_tag="PLO59_08700"
     CDS             <1..1078
                     /locus_tag="PLO59_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01225.1"
                     /translation="TWIANGAPDKNGKVFFANATSKYYISNQGCDLVAICDPERQVIA
                     RYIKVGISEGNIELPHTIKLAPDSKHWYAVFYAGNVFQKFRTSDDSLVGTANITVGGW
                     SSLVITDDSKTAFISDLINHAIVQINLDNMQQVGPFYNIYQNAHGLAINPITGWLYVT
                     NQYSNYINRALLSDLTSPECIPLFPGAPCADINSYNPHEILFTPDSSRYFLTCDFSNE
                     IRVLSSHNDSLLATIAVGSQPKEMSISNRHPYLFVTCGETLNYNIAYRGSVSVINYQT
                     NQLVKEIKEAFFQPHGIAVNDDKDVVYVSSRNADPNGPAPHHVSQCGGRNGFLSIIKM
                     NTLTVDPNYKNELSVDPYSIFYKK"
     gene            complement(1103..>1433)
                     /locus_tag="PLO59_08705"
     CDS             complement(1103..>1433)
                     /locus_tag="PLO59_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HQV01226.1"
                     /translation="KAVIKLYPKSTYNMRVIQQYRNYKSVLDKEPHIVNYAMEAIERT
                     GIKPVLSSVRGGTDGSRLSFMGLPCPNIFAGEHAFHGKHEWVSIQDMHIAVDTIINLC
                     MIYEEKA"
BASE COUNT          447 a          274 c          268 g          444 t
ORIGIN      
        1 aacctggatt gctaatggcg cacccgataa aaatggcaaa gtgttttttg ccaatgcaac
       61 atctaaatac tacatcagca atcagggatg cgatttagtt gctatctgtg atccggagcg
      121 gcaggtaata gcacggtaca ttaaagtggg catatctgaa ggaaatattg aactgccaca
      181 taccataaaa ttagcgcctg atagcaaaca ttggtatgct gtcttttatg ccggtaatgt
      241 ttttcaaaaa ttcagaactt cagatgattc attagtaggt actgcaaata ttactgttgg
      301 tggttggagc agtttggtta ttactgatga ttcgaaaaca gcttttatat ccgatttaat
      361 taatcatgca attgttcaga ttaacctgga caatatgcaa caagtagggc ctttttacaa
      421 tatatatcaa aatgcgcacg gcttggctat taatccgata acggggtggc tatacgtaac
      481 taatcaatat agtaattata ttaaccgcgc tttgcttagc gaccttacat cgcccgagtg
      541 tattccttta tttccgggtg caccttgtgc cgatattaat tcgtataatc cacatgaaat
      601 attatttaca cctgatagca gccgttactt tttaacttgt gatttcagta atgaaataag
      661 ggttttaagc tcgcataacg atagtttgtt ggcaactatt gccgtaggca gtcaaccaaa
      721 agaaatgagc attagcaaca ggcatcctta tttgtttgta acctgtggcg aaacgcttaa
      781 ctataatatt gcataccgtg gttcagtatc cgttataaac taccaaacca atcagttggt
      841 taaagaaatt aaagaagcat tttttcaacc acatggtatt gctgtaaatg atgataaaga
      901 tgtggtttat gtttcgagcc gtaatgccga ccctaacggg ccagcgccac atcatgtgag
      961 tcaatgtggt ggtcgcaatg gttttttatc aataataaaa atgaatacat taacggttga
     1021 cccaaattat aaaaacgaat tatcggttga cccctactcc attttttaca aaaagtaaat
     1081 tgtatagcac ctaaggaata agtcaggctt tctcttcata aatcatacac aaatttatga
     1141 tggtatcaac tgctatatgc atatcctgaa tactcaccca ttcatgcttg ccatgaaaag
     1201 catgctcacc tgcaaatata ttggggcaag gcaagcccat aaaagacaag cgagaaccat
     1261 ctgtaccgcc tcttacgctt gaaagcaccg gcttaatgcc tgtgcgttca atagcctcca
     1321 ttgcataatt aacaatatgt ggttctttat ccagaactga tttataattt cgatattgtt
     1381 ggataacccg catattatac gttgatttag ggtacaactt tataactgct ttt
//
LOCUS       DAOMAA010000670         1432 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_108030_length_1432_cov_2.616558, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000670 DAOMAA010000000
VERSION     DAOMAA010000670.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108030_length_1432_cov_2.616558"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1048
                     /locus_tag="PLO59_08710"
     CDS             <1..1048
                     /locus_tag="PLO59_08710"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HQV01227.1"
                     /translation="QQTKNLNTFSIGFSDAPHFDETYFANLVAKKHQTNHTVFNVTQK
                     NLFEILNDVLDYTDEPFADSSALNVFILSKLTRQKATVALSGDGADEIFAGYQKHRAE
                     WILQNEPQKTQAINIAAGLLKSFSGSRNSKLGNKIRQIHRLAEGSRLNTANRYWRWCS
                     LASTLQAEKLMPLSASQFLKYNNRKNKIIKNIGIDFNDILLTDMQLVLPNDMLYKVDM
                     MSMANGLEVRTPFLDFELVDFAFTLPANYKIDRFNQKKILKDAFKHLLPDEILKRSKK
                     GFEVPLLTWFQTDLKTTINELLDDTFILEQGLFNIIEVQHLKRQLFSNNPGEIQGRIW
                     ALIVFQHWYKKFFL"
     gene            1135..>1432
                     /locus_tag="PLO59_08715"
     CDS             1135..>1432
                     /locus_tag="PLO59_08715"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HQV01228.1"
                     /translation="MNAFTIDFEDWYQGVELPYSDWPKYEKRVEIGFYKIFEMLNQKN
                     IKATWFTLGWVAQNYPHLIKELHNAGHELASHGYKHDLAYKLTPEMFKEDVSKTK"
BASE COUNT          503 a          247 c          248 g          434 t
ORIGIN      
        1 acaacaaacc aaaaacctga atactttctc gataggcttt agcgatgcgc ctcactttga
       61 cgaaacctat tttgcaaatt tggttgcaaa aaaacatcaa acaaaccata ccgtttttaa
      121 tgtaacccaa aaaaatctct tcgaaattct taacgatgta ttggattaca ccgatgaacc
      181 atttgccgac tcatcggcct taaatgtttt tattctgagt aagcttacac gtcaaaaagc
      241 cacggttgct ttaagtggcg atggtgctga cgaaatattt gcaggctacc aaaaacaccg
      301 tgccgaatgg atactgcaaa acgaaccaca aaaaacacag gctataaata ttgctgccgg
      361 tttgctaaaa agtttttcgg ggtcaagaaa cagtaagttg ggtaataaaa taagacaaat
      421 acaccgcttg gccgaaggaa gcagacttaa tactgccaat cgttattggc gttggtgcag
      481 tttggcatca accttacaag ccgaaaaact tatgccttta agtgcatccc aatttttaaa
      541 atataacaac agaaaaaaca agattattaa aaacataggc attgacttta atgatatatt
      601 attaaccgat atgcaattgg ttttacctaa cgatatgttg tataaggttg atatgatgag
      661 tatggctaat gggctggaag ttcgtacgcc attcttagat tttgaattgg ttgattttgc
      721 atttacactc cctgcaaatt ataaaattga ccggtttaat cagaaaaaaa tacttaagga
      781 tgcgtttaaa catttactgc ctgatgaaat tttaaaacga agtaaaaagg gatttgaagt
      841 accattactt acctggtttc aaaccgattt aaaaactact ataaatgagt tattggatga
      901 tactttcata ttagaacaag gtctttttaa tattatagaa gttcagcatc ttaaaaggca
      961 attattttcg aacaaccctg gcgaaattca aggacgtata tgggcattaa tagttttcca
     1021 acattggtat aaaaagtttt ttctttaaaa ttttgttcaa ttaattatga gtttatttat
     1081 tgtgcaataa ttaccactca caacctacct aaatttttta attccaccag cactatgaat
     1141 gcttttacca ttgactttga agattggtat caaggcgttg aactgccgta tagcgattgg
     1201 cccaaatatg aaaagcgtgt cgaaattggc ttctataaga tttttgagat gttgaatcaa
     1261 aaaaatataa aagcaacttg gtttacattg ggttgggttg cgcaaaacta cccgcacctt
     1321 ataaaggaac tacataatgc cggccatgaa ttagcatcgc atggctacaa acatgattta
     1381 gcttataagc taacaccgga aatgtttaaa gaagatgttt caaaaacaaa aa
//
LOCUS       DAOMAA010000671         1432 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_108057_length_1432_cov_2.319535, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000671 DAOMAA010000000
VERSION     DAOMAA010000671.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108057_length_1432_cov_2.319535"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="PLO59_08720"
     CDS             complement(<1..534)
                     /locus_tag="PLO59_08720"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQV01229.1"
                     /translation="MSKIGLIISREYITRVRKKSFIIMTLLGPLLMGGLLAAVAYMAI
                     NSNDNVSVIQVVDQTPNQIISSKLVSTATLIYINTTTSVDSLRAHFDSDKTYGILHIP
                     ANLTNDQSNINFYTEKKANLSVVSAIEKDLQKQLEAAKLRNAGIDPVILASIKTDVSI
                     KQKTISGIEESNAEASAI"
     gene            complement(551..>1432)
                     /locus_tag="PLO59_08725"
     CDS             complement(551..>1432)
                     /locus_tag="PLO59_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020598354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQV01230.1"
                     /translation="NHQALSDVSIEVESGAVFGLLGPNGAGKTSLIRIINQITGPDTG
                     QILLNGETLQPAHIAQIGYLPEERGLYKKMEVGEQALYLAQLKGMSRIDAMRELKFWF
                     EKFEMTGWWKKKIEELSKGMAQKVQFITTVVHKPKLLILDEPFTGFDPINANLIKDEL
                     LELKSKGVTTILSTHRMESVEELCTHIALINKSYKVLDGSVKDIRKKYKSNIYEITYQ
                     GNQVSLANSLWGNFELLSNQPIEDGMLARIKLLDDTKPNHLLGQLIQNIEIVSFKEHV
                     PGMNEIFIECVGTSNEL"
BASE COUNT          377 a          268 c          273 g          514 t
ORIGIN      
        1 aatggcactt gcttcggcat tactctcttc gattccggat atggtttttt gttttataga
       61 aacatcggtt tttatagatg caagtataac agggtcgata ccggcattgc gcaatttggc
      121 ggcttctaat tgtttttgta aatccttttc aatagccgat accacactca ggttggcttt
      181 cttttcagta taaaaattaa tattcgattg gtcgttagtt aagttagccg gtatgtgtaa
      241 aatgccgtac gttttatcgc tatcaaagtg tgcacgcaaa ctatcaaccg atgttgtggt
      301 gttgatatag attaaagttg ctgtacttac cagtttactg cttataattt ggttgggtgt
      361 ttgatcaaca acctgaataa ccgatacatt gtcgttactg tttatagcca tataggcaac
      421 ggctgctaac aaaccaccca tcaataaagg ccccaaaagg gtcatgatga taaatgattt
      481 ttttctgaca cgggtaatgt attcgcggct gatgattaat cctattttac tcataaaatg
      541 tttggttgta ttataattcg tttgatgtgc ctacacattc gataaaaatt tcattcatgc
      601 cgggtacatg ttctttaaac gaaactattt caatattttg tattagctgg cctaataaat
      661 ggttgggctt cgtatcgtct aataacttaa tgcgtgcaag cataccatct tcaatgggtt
      721 gattggataa taattcgaaa ttaccccaaa ggctattggc taatgaaacc tgattgccct
      781 gataggttat ctcgtaaatg ttggatttgt attttttacg aatgtctttc acgctgccat
      841 ctaaaacctt atacgatttg tttatcaatg caatatgtgt acacaattct tctaccgatt
      901 ccatacggtg tgtacttaat atagttgtta cacctttcga ttttagttcc agtaattcgt
      961 cttttatcag attggcattt atagggtcga aaccggtaaa tggttcgtct aaaattaaaa
     1021 gtttgggctt gtgtactacg gtagttataa actgtacttt ttgagccatg cctttactta
     1081 gctcttcaat ttttttcttc caccaaccgg tcatttcaaa tttttcgaac caaaatttta
     1141 gttcacgcat ggcatctata cggctcatgc cttttagttg tgccaggtat aaagcctgtt
     1201 cgcctacttc cattttttta tacaggccac gttcttcggg caagtagcca atttgggcaa
     1261 tatgtgccgg ttgtaacgtt tctccgttta ataaaatttg acccgtatcg gggccggtaa
     1321 tttgatttat aattcgaatg agtgaggttt tgccggcacc atttgggcct aataatccaa
     1381 atacggcacc ggattcaact tcgatgctta catcgcttaa ggcttggtga tt
//
LOCUS       DAOMAA010000672         1431 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_108180_length_1431_cov_2.529070, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000672 DAOMAA010000000
VERSION     DAOMAA010000672.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108180_length_1431_cov_2.529070"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="PLO59_08730"
     CDS             <1..394
                     /locus_tag="PLO59_08730"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HQV01231.1"
                     /translation="SQQITVQVDSVTKAMAGYYFVQLTNGVCAITDSVMVKVADCNLL
                     LPNVLTPNNDGYNDTWQLANDAVSFWQLQVFNRWGNTVFNADGKKVIWDGRDNHGQLL
                     SEGTYYYLLHYVFNGYAKTMKGHFSLFR"
     gene            complement(488..>1431)
                     /locus_tag="PLO59_08735"
     CDS             complement(488..>1431)
                     /locus_tag="PLO59_08735"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681511.1"
                     /note="biotin-containing enzyme that catalyzes a two step
                     carboxylation of pyruvate to oxaloacetate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HQV01232.1"
                     /translation="HEIPGGQYSNLKPQAISLGLQHRMEEIKKAYEEVNLLFGDIVKV
                     TPSSKVVGDMALYMVTNNLSTADIFSKGESLSFPESVKSMFRGDLGQPHGGWPADLQK
                     IILKDEKPYQDLPNAHLAPIDFDAAFEEFRASHDQSLTFLDFLSYMFYPKVFDAYYKH
                     QKQYGDVSMVPTPAFFYGMKNNEEILVEIGKGKTLIIRLLYIGDADENGLRTVYFRLN
                     GQTRAIEVKDQKLNIKKETHVKATDTAKHVGSPMQGMLSKIFVKPGDAVQKNQPLFTI
                     EAMKMETTITAPRDMVVINIVLPERTLVEQDDLILEV"
BASE COUNT          413 a          268 c          270 g          480 t
ORIGIN      
        1 tagtcagcaa attacggtgc aagttgattc ggttacgaaa gcaatggcag gttattattt
       61 tgtacagcta actaatggtg tatgcgcaat taccgattcg gttatggtaa aggttgcaga
      121 ttgtaatttg ttactaccta atgtacttac acctaacaac gatggctata acgatacctg
      181 gcaattagca aacgatgctg taagtttttg gcaattgcag gtatttaacc gatggggtaa
      241 taccgtgttt aatgccgatg gtaaaaaagt tatttgggat gggcgtgata accatggtca
      301 attgctatcc gaaggcactt actactacct gctgcattat gtttttaatg gctatgcaaa
      361 aacaatgaaa ggacactttt cactattcag gtagttgggt ttattataaa tgcactaaca
      421 ttttgtgcat tcaaaaaaac agcacttgca aaataattgc aagtgctgtt taaatattcg
      481 ttttctgtta cacttctaaa atcaaatcat cctgttctac caatgtgcgt tcgggcaaaa
      541 caatatttat tacaaccata tcgcgtgggg ctgtaatggt tgtttccatt ttcatagctt
      601 caatagtaaa caagggttgg tttttttgaa ctgcatcacc gggctttaca aatattttgc
      661 ttaacatacc ttgcataggc gagcctacat gtttggccgt atcggttgcc ttaacatggg
      721 tttccttttt aatattcaac ttttgatctt taacttcgat agcacgtgtt tggccattga
      781 gtctgaagta aaccgtacgt aaaccatttt catcggcatc gccaatgtaa agcaacctga
      841 taattaatgt tttgcctttg ccaatttcca ccaatatttc ttcattgttt ttcattccgt
      901 agaagaatgc aggtgtaggc accatgctaa catcgccata ttgtttttga tgtttataat
      961 acgcatcaaa tactttggga taaaacatgt acgataagaa gtcgagaaag gttaacgatt
     1021 gatcatgact tgctctgaac tcttcaaagg cagcatcaaa atcaatgggt gctaaatgtg
     1081 cattgggtaa atcctgataa ggcttttcgt cttttaaaat tattttttgc aaatcagccg
     1141 gccatccacc atgtggttgc cctaaatcgc cacggaacat actttttaca ctttcaggaa
     1201 acgataaact ttcgccttta ctaaaaatat ctgcggtact aagattgttg gttaccatgt
     1261 acaaggccat atcacccact acttttgatg agggtgtaac ttttacgata tcaccaaaaa
     1321 gtaagtttac ctcttcatac gcttttttaa tttcttccat acggtgttgt aaacctaacg
     1381 atatggcttg tggctttaaa ttagagtatt ggccacccgg aatttcgtgt t
//
LOCUS       DAOMAA010000673         1430 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_108277_length_1430_cov_3.845091, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000673 DAOMAA010000000
VERSION     DAOMAA010000673.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108277_length_1430_cov_3.845091"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="PLO59_08740"
     CDS             <1..427
                     /locus_tag="PLO59_08740"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV01233.1"
                     /translation="VPMSYLINNSIGKNGQIINVVPSQNLVVIRMGNAPYTNSNAPTV
                     FNDSIWIILNQIICTANAISEPTSFRLNVFPNPAADLVNVEVTMPIEQFEIYNNWGVL
                     LHCFKPVKSTFTLPNLNSGIYLLKLKTAGKVYYKRIIKI"
     gene            complement(543..>1430)
                     /locus_tag="PLO59_08745"
     CDS             complement(543..>1430)
                     /locus_tag="PLO59_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HQV01234.1"
                     /translation="KTTLSADPNRGLIGDDEHGWSDKTVFNFEGGCYAKCVNLSAEKE
                     PEIFKAVKFGALLENIEFYEGTTQVDYDNISKTENTRVAYPINHIENAVEPSVAPSPK
                     NIFFLTCDAFGVLPPISRLTIPQAMYHFISGYTAKVAGTEVGVTEPTTTFSACFGKVF
                     LPLHPGKYAELLGAKLRNDTSIKVWLVNTGWSGGSYGTGSRMKLSYTRAMISAALNGE
                     LDKVAFEKLPVFGLEMPTTCPNVPVEILNPRNTWADKAAYDAKAIDLANKFIKNFEQY
                     ASGASKETLDAAPKLAIEA"
BASE COUNT          426 a          271 c          252 g          481 t
ORIGIN      
        1 ggtccctatg tcgtatttaa taaacaactc aataggtaag aatggacaaa ttattaatgt
       61 ggttccgtct cagaatttgg ttgttatacg aatgggaaat gcaccctata caaattcaaa
      121 tgcacctact gtattcaatg attctatatg gattatatta aatcaaataa tttgtactgc
      181 taatgcgata agcgaaccaa catcattcag gcttaatgtt tttccaaacc cggcagcaga
      241 tcttgttaat gttgaagtaa caatgcccat cgaacaattt gaaatttata ataattgggg
      301 cgtcttatta cactgtttta aacctgttaa atcaacattt acattgccaa atcttaatag
      361 tggcatttac cttttaaaat taaaaacagc cggtaaggtt tattataaac gcattattaa
      421 aatctgattt aatgttcgga attaaatagc ttttggctgt tttttttaaa ataatctatc
      481 gaaaacaaaa agagcctgct aatattttag caagctcttt ttttatcaca cagcatttgt
      541 aattatgctt caatagctaa tttgggtgca gcatctaaag tttccttact ggcccctgaa
      601 gcatattgtt caaagttttt aataaactta ttagcaaggt caattgcttt cgcatcataa
      661 gctgctttat cggcccaggt gttgcgcggg tttaaaattt ctaccggtac attagggcat
      721 gtggttggca tttctaaacc aaaaacaggt aatttttcaa aggcaacttt atctaattca
      781 ccgtttaatg ctgccgatat cattgcacgc gtataactaa gtttcatacg gctgcctgtg
      841 ccataagagc cgccactcca gccggtatta accaaccaaa ctttaatgct ggtatcgttt
      901 cttaattttg cgcctaataa ttcggcatac ttgcccggat gtagtggtag aaacacttta
      961 ccaaagcatg cgctaaaagt ggttgttggt tcggtaacgc ctacttcggt accggcaacc
     1021 tttgcagtgt agccacttat aaaatgatac atagcttgtg gtatggttaa cctgcttatt
     1081 ggaggcaaca ctccaaaggc atcgcatgtt aaaaagaaaa tattttttgg tgaaggagct
     1141 accgatggtt caacggcatt ttcgatatga tttatggggt aggcaacacg tgtattttca
     1201 gttttgctta tgttgtcata atcaacctgt gtagtgcctt cataaaattc aatgttttct
     1261 aacaaggcgc caaacttaac tgctttaaat atttcgggtt ctttttcggc acttaaattt
     1321 acacactttg catagcaacc accttcaaag ttaaatacgg ttttatcgct ccatccatgt
     1381 tcatcatcgc ctattaaacc acggtttgga tcggcactta atgttgtttt
//
LOCUS       DAOMAA010000674         1427 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_108771_length_1427_cov_2.529883, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000674 DAOMAA010000000
VERSION     DAOMAA010000674.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1427
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108771_length_1427_cov_2.529883"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1427)
                     /locus_tag="PLO59_08750"
     CDS             complement(<1..>1427)
                     /locus_tag="PLO59_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01235.1"
                     /translation="NIRARAATQITKDLYDDSEPHFTNNMQRIIFTSNRSNDTIGIGD
                     FYTDAQKNSDVFLYDYATRSNILARITKTPAISEHQPMESDSGRYVFLSDAFGVRNRM
                     LATVDSTISFVDTTEHYRFIVRQFNQTNYARSILQHDVSYKHNWYAEKFIKNGKYQIV
                     VNPLPPSLLATTNEVFTESQQPTVAFVPVIKTATPADTAATDLTPFYYQSEFKSKPGK
                     TTNNNPTADTAAQQIPFGILPGTQTVIKTVNNDSVIPYRLPKQRNYDLSFNMSYFVTQ
                     LDNNLLNPTYQAYTGGSEFYFGPGLNGLFLVGINELMEDYKLIGGMRVSGNLNSNEFY
                     VSYENLRHRFDKTWSFYRQAREDFIGYAPIKILTQELRYKIKYPFNDVSSLRGSFGFR
                     ADRGVVRSVDQPTLQFPNEYLYWGTAKLEYVFDNTINRGVNLLQGTRCKIFAEAFNQV
                     DELETFLGVAGIDYRHYTRISRQII"
BASE COUNT          410 a          271 c          292 g          454 t
ORIGIN      
        1 caaataattt ggcgcgatat gcgtgtgtaa tgtctgtaat caatacctgc aacgcctaaa
       61 aaagtttcca actcgtcaac ctgattaaag gcttcggcaa atattttaca gcgtgtaccc
      121 tgcaataaat ttacaccccg gtttatggtg ttgtcaaata catattctag tttggctgtg
      181 ccccaataca aatattcgtt tggaaattgc agggtaggct ggtcaacgct tcgtaccaca
      241 ccacggtcgg ccctgaaacc aaagctgcct cgtaaactgg aaacgtcatt aaacggatac
      301 ttaattttat agcgcagctc ttgggttaaa attttaatgg gtgcatagcc aataaagtct
      361 tcgcgagctt gacgataaaa cgaccaggtt ttgtcgaaac gatgacgcag gttttcataa
      421 ctcacgtaaa actcattgct gtttaaatta cccgaaaccc gcatgcctcc aattaactta
      481 tagtcttcca tcagttcatt tatacctact aaaaacaagc cgtttaagcc ggggccaaaa
      541 taaaattcgc taccgccagt atatgcctga taggttggat taagcaaatt gttatccaac
      601 tgtgttacaa aatagctcat gttaaacgat aaatcgtaat tgcgttgctt gggtaagcgg
      661 tagggtataa cactgtcgtt atttacggtt ttaattactg tttgcgtacc gggtaaaata
      721 ccgaatggga tttgttgcgc tgctgtatcg gcagtagggt tgttgttagt tgttttaccg
      781 ggtttcgatt taaattcgct ttgataataa aagggcgtta aatcggttgc agcagtatca
      841 gcaggtgtag ctgtttttat aaccggtaca aaagcaacag taggttgttg cgattcggta
      901 aatacttcgt tggtagttgc taataatgat ggtggtaacg gatttacaac aatttgatat
      961 ttaccgtttt taataaattt ttcggcatac cagttatgct tgtagctaac atcatgttgt
     1021 aaaatactgc gtgcatagtt tgtttgatta aactgtcgaa ctataaaacg gtaatgttct
     1081 gtagtatcaa caaaactaat agtgctatct acagtagcca gcatacggtt tcgaacgcca
     1141 aaagcatcag ataaaaaaac atagcgtccg ctatccgatt ccataggttg gtgttcgcta
     1201 attgcaggtg tttttgttat acgcgccaat atgttactgc gcgttgcata atcatataaa
     1261 aacacatcgc tgtttttttg tgcatctgta taaaagtcgc caatgccaat ggtatcatta
     1321 cttctgttac tggtaaaaat aatgcgctgc atattattgg taaagtgcgg ctcactatca
     1381 tcgtataggt ccttagttat ctgtgttgca gcccttgctc tgatatt
//
LOCUS       DAOMAA010000675         1426 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_108888_length_1426_cov_2.574034, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000675 DAOMAA010000000
VERSION     DAOMAA010000675.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108888_length_1426_cov_2.574034"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1197
                     /locus_tag="PLO59_08755"
     CDS             <1..1197
                     /locus_tag="PLO59_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQV01236.1"
                     /translation="EAIENFKLEADQAERSGDYGKVAEIRYGRIKDAEQKVTDLQLKL
                     TAMQTAGAMMKEEVDSEDIADVVARWTGIPVKRMLQSEREKLLHLEDELHKRVIGQED
                     AIAAVSEAVRRNRAGLSDAKRPVGSFIFLGTTGVGKTELAKALADYLFNDENSLVRID
                     MSEYQERHTVSRLIGAPPGYVGYDEGGQLTEAVRRRPFSVVLLDEIEKAHPDVFNILL
                     QVLDDGRLTDNKGRIANFKNTIIIMTSNTGAHIIQDNFEKLTDVNRDEVIAKTKNQVF
                     EVLKKNIRPEFLNRIDEIIMFAPLTRDEIKKIVGIQLQHIKQMLAGNGITLTITDNAV
                     DWLAQLGYDPTYGARPLKRVMQKKVLNELSKKIIDGSINKDDVISIDVNDKKEFVFSN
                     AVAHIA"
BASE COUNT          479 a          250 c          304 g          393 t
ORIGIN      
        1 gaagctatcg aaaattttaa actcgaagcc gatcaggccg agcgcagtgg cgattacgga
       61 aaggtggccg aaatacgata tggccgcata aaagatgcag aacaaaaagt aaccgatttg
      121 caattaaaat taactgcaat gcaaactgca ggtgccatga tgaaagaaga agtagatagc
      181 gaagatattg ccgatgtagt agcccgctgg accggtattc cggttaaacg aatgctgcaa
      241 agcgaacgcg aaaaattatt acacctcgaa gatgaattac acaagcgtgt tattggtcag
      301 gaagatgcca ttgctgccgt tagcgaagcc gtgcgtagaa accgtgccgg ccttagcgat
      361 gccaaacgtc cggtaggttc gtttatattt ttaggtacta ccggtgtggg taaaacggaa
      421 ttagccaaag ccctggccga ctatttattt aatgacgaaa atagtttggt tcgtattgat
      481 atgagcgagt atcaggagcg gcatactgta agcaggttaa ttggtgcacc gcccggatat
      541 gtaggttatg atgaaggtgg acaacttact gaggccgtac gcagacgacc ttttagtgta
      601 gtgctgcttg atgaaattga aaaagcacat cccgatgttt ttaatatttt attgcaggtt
      661 ttagatgatg gaagacttac cgataataaa ggccgtattg caaattttaa aaacaccatc
      721 attataatga cttcaaatac gggtgcacat attatacagg ataattttga aaaattaacg
      781 gatgtaaacc gcgatgaagt aattgccaaa accaaaaacc aggtttttga agtactaaag
      841 aaaaatatca ggcctgagtt tttaaaccgt attgacgaaa ttatcatgtt tgctccgctt
      901 acgcgtgatg aaattaaaaa aattgttggt attcagttgc aacacatcaa acaaatgctg
      961 gcaggcaatg gcataacgtt aactattacc gataatgctg ttgattggtt ggcacaattg
     1021 ggttacgacc ctacttatgg agccaggccg ctaaaacgtg ttatgcagaa aaaagtgctc
     1081 aatgaattat cgaaaaaaat aattgacggc agtatcaata aagatgatgt aataagcatt
     1141 gatgtaaacg ataaaaaaga atttgttttc agtaatgccg tagcacatat tgcctaatgc
     1201 agcaattgcc ggcatgaacc attaaaaaat attggtttat taaatttgta ttacctatta
     1261 agtggataaa gatttagaat agaaagaagc tgtctcgcat ttgcatttcg agacagcttc
     1321 tttttttttt caattttcta tgctaaaatt tttgcgtatt aactcagtta aacaagcaaa
     1381 agtgctttcc atctaaccac tctccactaa ccactaacca ctaatc
//
LOCUS       DAOMAA010000676         1421 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_109587_length_1421_cov_3.595168, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000676 DAOMAA010000000
VERSION     DAOMAA010000676.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109587_length_1421_cov_3.595168"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="PLO59_08760"
     CDS             <1..665
                     /locus_tag="PLO59_08760"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV01237.1"
                     /translation="TVYLSTTSHACNTIQWYHNGQPLPGQNQTNLPVTQQGIYTVELS
                     YYGNNAKTSAGVNIVVNPQPVIITASSGVLSAPLGGSYQWWFSPNGNSFTSIPAANAV
                     SYQATATGFYYVHFTDANGCSDTSNHLYVGFTGIAQNTYNTITLTPNPSTQIITLTNL
                     SAKSITQVTIYNTLGVEVIHHLIQEKTENVLLNIAQLPAGIYFVKVKKGGKQTLLSFI
                     KN"
     gene            1105..>1421
                     /locus_tag="PLO59_08765"
     CDS             1105..>1421
                     /locus_tag="PLO59_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018344774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HQV01238.1"
                     /translation="MNVDVRNSKADWGPFTRKQAPEGSPNILFILYDDTGLATWSPYG
                     GRINMPTLDKLAADGLTYTQWHTVALCSPTRSCILTGRNHNQNGMGCITEGANGFPGY
                     SCQL"
BASE COUNT          460 a          303 c          256 g          402 t
ORIGIN      
        1 atactgttta tttaagcaca acatcacatg cttgtaacac cattcaatgg tatcacaatg
       61 gacagccttt accgggtcaa aaccaaacaa atttgccggt aactcaacaa ggtatttaca
      121 ctgttgagtt aagctattat ggtaataatg caaaaacttc tgccggtgta aatattgtcg
      181 ttaatcccca gcccgtaatt attacagcca gcagcggtgt actatcagcg ccattgggcg
      241 gctcatacca atggtggttt agccctaacg gaaactcctt tacttcaata ccggctgcca
      301 atgctgtcag ctatcaggca acagctaccg gtttttacta cgtacatttt acagatgcaa
      361 acggttgcag cgataccagt aatcacctat atgttggttt tacaggtatc gcacaaaata
      421 catataacac tatcacgtta actcccaatc catcaacaca aataattaca ttaactaacc
      481 taagcgccaa aagcataacg caagttacca tttacaacac attaggagtt gaagtaatac
      541 atcacctgat acaagagaaa accgaaaatg tccttttaaa catagcgcaa ctgccagcag
      601 gtatatactt cgttaaggta aaaaaaggcg gtaaacaaac cttgctttcg tttataaaaa
      661 attaatgact gctactgccg cacaagttga tgctgctgtt tactgttgtt atttgtgtag
      721 tccgtataaa aacaaattta aaggttgtgc ctaatggtat gtttcttttt ggcttaacct
      781 taatacagtt tttgttaaat gtattaacac cattttgtac caagtattca ttgtaaagca
      841 acacgctttt agttacaaca ttaagggaaa cccagccaaa tcttttggtt gaagaaaaaa
      901 tttcagtcga gtcgattttg ttctacacaa attggatgca aatgaattaa cattgtaacc
      961 tcaacctttc aaacaaaaaa acttatgaaa caatttatcc tcacaacagc cataagcgtg
     1021 atgctggtaa gttgcagtta aaacacaaaa accggggaaa caggcaagag cacatctgaa
     1081 aattcaaatg gctttaatgg cgagataaat gttgatgtgc gcaattcgaa agccgactgg
     1141 ggcccattta cacgtaaaca agcgcccgag gggtcgccta atattttatt tatcctttat
     1201 gatgataccg gcttagccac ctggtcgcct tatggtggtc gtattaatat gcctactttg
     1261 gataaattag ctgccgatgg tttaacctat acacaatggc atactgtagc attatgttcg
     1321 ccaacacgct cctgcatact tacaggccgc aatcacaatc aaaacggcat gggttgcata
     1381 accgagggtg ctaacggatt tcctggctac agttgtcaac t
//
LOCUS       DAOMAA010000677         1421 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_109605_length_1421_cov_3.098097, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000677 DAOMAA010000000
VERSION     DAOMAA010000677.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109605_length_1421_cov_3.098097"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="PLO59_08770"
     CDS             <1..327
                     /locus_tag="PLO59_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019495362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HQV01239.1"
                     /translation="LLAESNVSYDKLVEMEDINPEFAQTDVVLVVGANDVVNPAAKTD
                     PSSPIFGMPILDVENAQHIIVNKRSMNAGYAGIENELFYNPKTSMLFGDAKDALSKLV
                     TELKTL"
     gene            806..>1421
                     /locus_tag="PLO59_08775"
     CDS             806..>1421
                     /locus_tag="PLO59_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013631301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HQV01240.1"
                     /translation="MTFDKFTTKAQESIMQAQQLTTSQGQQAIEAAHLLKGMMQADEN
                     VIPFLLKKLNVNIPQLEKGIETLMATYPKVSGGQVYMSNDGSQILTKALSMLKEFGDE
                     YVTTAHILLAMLQYKNKTGLLLKEQGLNEKDLHIAIIELRKGENVTSSSAEETYNALN
                     KYARNLNELAQAAKLDPVIGRDEEIRRVLQILSRRTKNNPILIGE"
BASE COUNT          492 a          248 c          259 g          422 t
ORIGIN      
        1 ttattagcag aatctaatgt aagttatgat aaattggtag aaatggaaga cataaacccc
       61 gagtttgcgc aaacagatgt agtgttagtt gttggtgcca atgatgtggt taaccctgct
      121 gccaaaaccg atccatcgtc acccattttt ggtatgccta ttttagatgt tgaaaatgca
      181 caacacatta ttgtaaacaa acgctcgatg aatgccggtt atgctggcat cgaaaacgaa
      241 ttgttttaca atcctaaaac cagcatgttg tttggtgatg caaaagatgc tttgtcgaaa
      301 ttggttaccg agcttaaaac tttatagtta cagcagggcc atttttttat tgataatttt
      361 attgcaaagc ttttaaaaca ggcatactgc ttctaagcta aatttaggta agctatttta
      421 aagctgcttg attatgtatg gtgttttatc ttttgcggtt tcaatttgta attaacttac
      481 acaatatttt ttaccggcaa gtttttaaaa aatcgaattc actgtttcag ttgactatct
      541 gtttttaaaa gcaacattac acaaaccgat agcttagcac tattaatgaa atgcattttg
      601 gttatgatct gacttttttt ctttgtgcat taattcaaaa agtaaagttg gctgtataac
      661 acaatcaaaa aagccatgtt ggcgtataat tttttgtggt taatcatttg agaacccttg
      721 gcaataccgg ttgcataaaa aagtaactgg taccaaaatc agcttttgca attgcataag
      781 cctcaaaaaa caaaaacaac taattatgac attcgataaa ttcacaacaa aagcacaaga
      841 gtcgattatg caggcacagc agctcacaac atcgcaaggc cagcaagcta tcgaagccgc
      901 acatttatta aaaggcatga tgcaggctga tgaaaatgta attccttttt tacttaaaaa
      961 attaaatgta aacatacctc aattagaaaa aggtatcgaa actttaatgg ctacctatcc
     1021 aaaagttagt ggcggtcagg tatatatgag taatgatgga agccagattt taaccaaagc
     1081 cttaagcatg cttaaggagt ttggcgatga atatgtaacc actgcacaca tactgttagc
     1141 catgttgcag tataaaaaca aaaccggttt actgcttaag gaacaaggtt taaacgaaaa
     1201 agatttacat atagccatta tcgagctgcg aaaaggcgaa aacgtaactt cttcaagtgc
     1261 cgaagaaact tacaatgcat taaataaata tgcacgtaat ttaaatgagc tggcacaagc
     1321 ggccaagctc gatccggtta ttggccgtga cgaagagata agacgtgtac tgcaaatatt
     1381 atcacgcaga accaaaaata acccaatcct cattggcgaa c
//
LOCUS       DAOMAA010000678         1421 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_109648_length_1421_cov_2.442899, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000678 DAOMAA010000000
VERSION     DAOMAA010000678.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109648_length_1421_cov_2.442899"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="PLO59_08780"
     CDS             <1..531
                     /locus_tag="PLO59_08780"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HQV01241.1"
                     /translation="VVFFTDTIEKFIPPKKGKSHILRIIRELINFAPASKNTDISQAL
                     RYFTNVIKKRSIAFVISDFIDSKIQNQNKVFEDALKIASKKHDLVALRIYDEREFYLP
                     DMGLITLADAETGNLTQVDSSSKQIRKQYEMFARKNETNLQNIFNKCGVDCASIRTDQ
                     NYVQPLIKLFKKREKR"
     gene            584..>1421
                     /locus_tag="PLO59_08785"
     CDS             584..>1421
                     /locus_tag="PLO59_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01242.1"
                     /translation="MLLIAGYTYAQQPHVSAKADTNAILIGQQIKIKLQATVPATYQL
                     QWPELTDSLQNFTITETGKIDTLLQNNLSTISQQIIVTTFDSGIHYIPALAFNFITAK
                     GQMPLAIFTDSIMVAVSSVAVDTSKEIRDIKPPIEVPYTFYDFLPYIIALIVLALIIY
                     FVYRYYLKQKRKQKTIVIQAPKRPAHEIALAALHELRNRKVWQQGDIKGYHTELTDIV
                     RTYIANRFKINAPEFTTDEVLQSIAIAQLTADTKNTLRYTLQLADLVKFAKAHPLPDE
                     QEQ"
BASE COUNT          517 a          292 c          225 g          387 t
ORIGIN      
        1 gtcgtatttt ttaccgatac catcgaaaaa tttataccac ccaaaaaagg gaaatcgcat
       61 atactgcgca tcatacgcga gcttattaat tttgcacccg cttcaaaaaa tacagatata
      121 tcgcaagcgc tgcgctactt taccaatgta attaaaaaaa ggagtattgc ctttgtaata
      181 tccgatttta ttgattctaa aattcaaaat caaaacaaag tttttgaaga tgcattaaaa
      241 attgcaagca aaaaacatga cttagtagcg ttgcgaattt atgatgagcg cgaattttat
      301 ttgcctgata tgggcttaat aactttagcc gatgccgaaa caggtaacct tacccaggtt
      361 gatagcagca gcaaacaaat cagaaaacaa tatgaaatgt ttgcacgtaa aaacgaaacc
      421 aatcttcaaa acatttttaa taaatgtggt gttgattgtg caagcattcg caccgaccaa
      481 aattatgtac aacctttaat taaacttttt aaaaagcgcg aaaaacgata gtgcttttat
      541 tgaaatgctt aacttcaaat acatattaaa attgcaactg gctatgctac ttatagccgg
      601 ttatacttat gcacaacaac cacacgttag cgctaaagcc gataccaatg ccatacttat
      661 tggccaacag ataaaaataa aattacaggc tacagtgccg gctacgtatc aattacaatg
      721 gccggaatta accgattcgc tgcaaaattt taccattacc gaaaccggaa aaattgatac
      781 gctgttacaa aataatctat caaccatatc acaacaaata attgtaacca ctttcgactc
      841 gggcatacat tacataccgg cactggcatt taatttcata acggcaaaag gtcaaatgcc
      901 attggcaatt tttactgata gcattatggt tgccgtaagt tcggttgcag ttgataccag
      961 taaagaaata cgcgatataa agccacccat tgaagtacca tacacctttt acgattttct
     1021 gccctacatc attgcattaa ttgtactggc gctgattata tattttgttt accgctatta
     1081 tctaaaacaa aaacgcaaac aaaaaaccat tgtaatacaa gcacccaaac gccctgcaca
     1141 cgaaattgct ttagcagctt tacatgaact acgcaaccgt aaagtatggc aacaaggcga
     1201 tattaaagga tatcataccg aactaactga tatagtaaga acctacattg ctaatcgttt
     1261 taaaataaat gcaccggaat ttacaaccga tgaagtactg caatcaattg ctattgcaca
     1321 gttaactgct gatacgaaaa acacattgcg atacacgctg caattagccg acttggtaaa
     1381 gtttgcaaaa gcccatccac tacctgatga gcaagagcaa a
//
LOCUS       DAOMAA010000679         1420 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_109731_length_1420_cov_3.625641, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000679 DAOMAA010000000
VERSION     DAOMAA010000679.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109731_length_1420_cov_3.625641"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            13..>1420
                     /locus_tag="PLO59_08790"
     CDS             13..>1420
                     /locus_tag="PLO59_08790"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HQV01243.1"
                     /translation="MLLVAQLQQIGHLSYGSTGLAGCWHHVDSLGNEYALVGTSKGLS
                     IVDVSNPAQPVERFAVPGITNNWREVKTFGNYAYVTNEGGGGLQIIDCSNLPASAPYT
                     TYVGDGAITGQLNTIHALHIDAGYLYLYGCSFWGGTAIICNLNNNPLAPEYMGHTPTN
                     YVHDGYVRNDTLYACEIYNGLLAVYDVTNKANPTLINSVTTPNAFTHNSWLNDAGTVV
                     FTTDETANSFLTAYDITDITNITELDRIQTAPGSNSIVHNTHTLNDYEIVSWYTEGVV
                     IVDASKPTNLVEVAKFDMSPFSGGTFNGAWGVYPYLPSGNLVVSDIEGGLYVLTPTYK
                     RACFLEGLVTDSTTGLPINAATIKVLPINIQSASNFLGNYSMGVADSGTYDIEVSAFG
                     YQTKTYTGISLTNGNTTILNAEINKVPTISVSGNITNAGNGLPVPNAKVRIKNSFLSL
                     DTVADANGNFSVSSIIPGN"
BASE COUNT          422 a          324 c          293 g          381 t
ORIGIN      
        1 ctctgtctgc ccatgctgct cgttgcccaa ctccaacaaa tcggccacct ttcctatggc
       61 agcaccggcc ttgcgggttg ttggcatcat gtggacagcc tcggcaacga atatgcgctc
      121 gtgggcacca gcaaaggcct gagtattgtg gacgtatcca acccggcgca gcccgtggaa
      181 cgcttcgccg tgccgggcat taccaacaac tggcgcgagg taaaaacatt tggtaactat
      241 gcatacgtta ctaacgaagg cggtggtggt ttacaaataa ttgattgtag caacctgcct
      301 gcatcagcac cttatacaac ttatgttggc gatggcgcta taacaggtca gcttaacacc
      361 atacatgcat tacatattga tgcaggctac ctgtatttat atggctgtag tttttgggga
      421 ggcactgcaa taatttgcaa ccttaataac aacccattgg ctcctgaata catggggcat
      481 acaccaacca attatgtgca tgatggctat gtacgtaacg atactttata tgcctgcgaa
      541 atttataatg gcttgcttgc tgtgtatgat gtaaccaata aagcaaaccc tacgcttata
      601 aattcagtaa ccactcccaa tgcatttacg cataactcct ggcttaacga tgcaggtacc
      661 gttgtgttta caaccgatga aacagccaat agctttttaa cagcttacga tattaccgac
      721 ataacaaaca ttaccgagtt agaccgcata caaacagcac cgggcagcaa ctcaatagta
      781 cacaatacac acacccttaa cgattatgaa atagtaagct ggtacaccga aggtgtggtt
      841 attgttgatg catcaaaacc aaccaactta gttgaagttg ctaagtttga tatgtcgcca
      901 tttagtggtg gtacttttaa tggtgcatgg ggtgtttatc catacttacc ttcaggcaat
      961 ttagtggtaa gcgatatcga aggcggctta tatgttttaa cccccactta caaacgtgct
     1021 tgctttttag aagggttggt tacagatagt accacaggct tacccataaa tgcagcaact
     1081 ataaaagtat tgccaattaa tatccaatcg gcttcaaact tcttaggcaa ttacagcatg
     1141 ggtgtagctg acagcggtac gtatgatata gaagtttcgg catttggtta ccaaacaaaa
     1201 acctatacag gtatttcgct aaccaatggc aatacaacaa ttttaaatgc ggaaataaat
     1261 aaggtaccaa ccatttcagt tagtggcaac attaccaatg ctggcaacgg cttaccggta
     1321 cccaatgcca aagtacgaat taaaaacagc ttccttagtt tagacaccgt agccgatgcc
     1381 aacggaaatt tttcggtaag cagtattata cctggcaact
//
LOCUS       DAOMAA010000680         1420 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_109823_length_1420_cov_2.057143, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000680 DAOMAA010000000
VERSION     DAOMAA010000680.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109823_length_1420_cov_2.057143"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(198..>1420)
                     /locus_tag="PLO59_08795"
     CDS             complement(198..>1420)
                     /locus_tag="PLO59_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01244.1"
                     /translation="RVHPLLFRFAFMVKVTAGFILTLIYTHYYTDRSTADIFKYFDDS
                     KIMYDLCWVDFELFLKMLFGFNDVALAAYYNQMIAWYDSDFIYNDNRLMIKLNCLMRF
                     VSFGNYYIHVVLICFIVFYGLTCLFKLFASELKSKFSGIYFCAFFIPSVVFWTSAVLK
                     DAAILFALSVFLFNYNKILINAASVKSYILLLLAILLLLTIKVYVFVLVLPGLIIVTL
                     LKRKFLINYNQFAVWVVTYFIYAVVIFSLKYFTPYNIVEQIAIKQHQFLKLAYQVHSG
                     SILKAYELSTSVSLIKAMPLAFCNTFLQPYVGNFKSAVITLASIENALCLLALIFVAF
                     HSRLKNAFITPWFFFSLFFVLSLYSLIGLIVPVAGALVRYKTIAMPFFAFILLIIADT
                     EKIKATFRKLKILN"
BASE COUNT          587 a          238 c          216 g          379 t
ORIGIN      
        1 attaaaaaac gatatagcat cacccccaaa actaaattgc agcatcaaaa aatattgaag
       61 caacccaacc cctactttag ttatgaaaaa aaaaaccaac cactgccatg cataaccgca
      121 tttaaaaaag tgcattttat aaataagcac gagtgctaaa gcaaaatgga ggaagaaaac
      181 gaggcattgc acccacatca gtttaggatt tttaattttc tgaaagttgc tttaattttt
      241 tcggtgtcgg caataattaa taatatgaat gcaaaaaaag gcatagcaat agttttgtat
      301 ctaaccaaag cgcctgcaac cggaacaatt agacctatta atgaataaag cgataaaaca
      361 aaaaacaaag aaaagaagaa ccacggtgta ataaatgcat tctttaacct gctatgaaaa
      421 gcaacaaaaa ttaaagctaa aagacacaag gcattttcga ttgaggctaa tgtaataaca
      481 gccgatttaa aattacctac ataaggttgt aaaaatgtat tacaaaatgc caacggcatt
      541 gccttaatta agctaacaga ggtgctaagt tcgtacgctt ttaaaatact gccactatga
      601 acctggtagg caagttttaa aaattgatgt tgcttgattg caatttgctc aactatattg
      661 taaggtgtaa aatatttaag cgaaaaaatt acaactgcgt atataaaata cgttactacc
      721 caaacagcaa actgattata attaattaaa aatttgcgtt tcaataaggt aactattatc
      781 aaaccgggta acaccagtac aaaaacatat accttaatag ttaataaaag aagtattgcc
      841 agcaatagca aaatataact cttaactgaa gctgcattaa taagtatttt gttataatta
      901 aataggaaaa cagatagcgc aaacagtatt gctgcatcct ttaacacagc actggtccaa
      961 aaaacaacag atggtataaa aaaggcgcaa aaataaatgc ctgaaaattt tgattttaac
     1021 tcagaagcaa acagtttaaa caaacatgtt aagccataaa aaacaatgaa gcagataagc
     1081 actacatgaa tatagtagtt gccaaatgaa acgaaacgca ttaagcaatt gagcttaatc
     1141 attagtcggt tgtcgttgta aataaaatcg gaatcatacc aggcaatcat ttggttataa
     1201 taagctgcaa gtgctacatc attaaaaccg aaaagcattt ttaaaaacaa ttcaaaatca
     1261 acccagcata aatcatacat tattttactg tcatcgaaat acttaaatat gtctgctgta
     1321 cttctatcgg tatagtagtg cgtataaatt aaagtgagaa taaatccggc tgttactttt
     1381 accataaatg caaatcgaaa caacaaaggg tgtactctgc
//
LOCUS       DAOMAA010000681         1419 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_109936_length_1419_cov_2.306452, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000681 DAOMAA010000000
VERSION     DAOMAA010000681.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109936_length_1419_cov_2.306452"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1419
                     /locus_tag="PLO59_08800"
     CDS             <1..>1419
                     /locus_tag="PLO59_08800"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV01245.1"
                     /translation="MWTQVGNTMFTQPTSFANVVYFWDEANGIVMGDPIGNPLKYELH
                     LTTDSGTTWTAVPPASLPTLTDPAEYGIVNLFDAVQGHFWFCTTYGDVYHTSDKGLTW
                     TKSVTGIPANNLSGGGRQDITSIAFTDSLNGIISQINASGVITMNTNDGGTTWTSITP
                     SGTFYGDLTGVPGSQIFVSVGSNGTVGFGSSFSLDNGLTWNDLSTGASHTCVDFVDSV
                     TGFSGEYITAGGPGGAWKFNGSFGFINCGSSQISAGVGTFADSLVCFGDTALYSVAGA
                     VAPTDGTVKGFAVIVSTADLMGSSTPLSNPSVVGGTGVTPFPSNPFNVSLVNDGTIFP
                     AGDYYFTPVVIGNATGTGNITSLVLDPACTTTGASAMVRILASGDPLCNTSSLIDNAG
                     SEVKTFFSNQTLNITINAAVSDKTAITVFDATGRVVLNKTVNTHVGANNFTLNASDLN
                     SGIYMIKVANSSNQVTNRGSIIF"
BASE COUNT          391 a          271 c          308 g          449 t
ORIGIN      
        1 atgtggactc aggttggtaa cactatgttt acgcaaccca catcatttgc taacgtggtt
       61 tatttttggg acgaagccaa tggtattgta atgggcgacc caattggcaa tcctttaaaa
      121 tatgaattgc acttaactac agactcaggt actacctgga ctgccgtacc acctgcaagt
      181 ttacctacat taaccgaccc tgctgaatat ggtattgtta atttatttga tgcagttcaa
      241 ggacattttt ggttttgcac cacatacgga gatgtgtatc atacaagtga taaaggctta
      301 acctggacta aatccgttac tggtatacct gcaaataatt tatcaggtgg tggccgtcag
      361 gatattacca gcattgcttt taccgatagc ttaaatggta ttatttcaca aataaatgcc
      421 agtggtgtaa taacaatgaa caccaatgat ggaggtacaa cctggacaag tattacccca
      481 agcggtactt tttatggtga cttaacgggt gttcccggct cacagatatt tgtaagtgta
      541 ggctctaatg gtacggtggg ttttggctca tcattttcat tggataatgg tttaacctgg
      601 aatgatttaa gcacgggtgc ttcgcacacc tgtgttgatt ttgttgacag tgttaccggt
      661 ttctcaggcg agtatattac agctggtggc cccggtggtg cctggaaatt taatggctcg
      721 tttggattta ttaattgcgg ttcaagtcaa ataagcgcag gtgttggtac ttttgccgat
      781 agcttggttt gttttggcga tactgcgcta tatagtgttg ccggagcagt agctccaacc
      841 gatggtactg ttaaaggttt tgcagtaatt gtttctacag cagatttaat gggatcgtca
      901 acacctttat caaatccatc ggttgttggt ggtaccggtg ttacgccatt cccttcaaat
      961 ccttttaatg taagtttggt aaacgatggc actattttcc ctgctggcga ttattatttt
     1021 acaccggtag ttattggtaa tgcaacaggt acaggcaata tcaccagttt ggtattagac
     1081 ccagcatgta ctactacagg cgcttcggca atggtacgta ttttagcttc cggagaccct
     1141 ttatgtaaca caagcagtct tattgataat gcaggctctg aagtaaaaac atttttcagt
     1201 aatcaaactt taaacattac aataaatgca gcagtaagcg acaaaacggc tataactgta
     1261 tttgatgcaa ccggccgtgt agtattaaac aaaacggtaa atacacatgt tggtgctaac
     1321 aattttactt taaatgccag cgatttaaat agtggtattt atatgattaa agtagccaat
     1381 agcagcaacc aggttaccaa tcgcggttcc atcatcttc
//
LOCUS       DAOMAA010000682         1417 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_110190_length_1417_cov_3.144640, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000682 DAOMAA010000000
VERSION     DAOMAA010000682.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110190_length_1417_cov_3.144640"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="PLO59_08805"
     CDS             <1..698
                     /locus_tag="PLO59_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013073598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HQV01246.1"
                     /translation="TLVMLEIVLGVDNIIFIAIICGRLPIENQKRARTIGLMLALIMR
                     VLLLMLITEIASMTNPLFYIKEFGVSGRDLILFAGGWFLIIKTIIEINHKFKIADDKT
                     YHEKKITMMAAIIQIIIIDLVFSFDSILTAVGLSSQIIIMILAVVVSMFVMLFFAKYV
                     SDFVNKYPTVKMLALCFLVVIGALLVIESLHIHIEGIDFKSYAYVAMAFSLIVELLNI
                     RLRNVLDKKANDD"
     gene            complement(695..1159)
                     /locus_tag="PLO59_08810"
     CDS             complement(695..1159)
                     /locus_tag="PLO59_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATPase"
                     /protein_id="HQV01247.1"
                     /translation="MYISFNELPDTSRIWIYQCNRFLNEAEGKLLTSKTIDFIENWTA
                     HNNLLQASFNWVDNLFLVIAVNQQVNDASGCSIDKSVHFVQQLEQAFNVHFFDRMQVA
                     IKTDNEIKLMPLHLLSNAPENAKMYDNLIETVGAFKNEWLKPVAQSWAKQFV"
BASE COUNT          443 a          236 c          238 g          500 t
ORIGIN      
        1 ttaccctggt aatgctcgaa atagtactag gtgttgacaa catcattttt attgccatta
       61 tatgcggccg cttaccaatc gaaaatcaaa agagagcccg cactattggt ttaatgctgg
      121 ctttgattat gcgggtttta ttgctaatgc ttataacaga aatagcaagc atgaccaatc
      181 cgctatttta tataaaagag tttggtgtaa gtggccgtga tttaattttg tttgccgggg
      241 gctggtttct gattataaaa accatcatag aaataaatca taagtttaaa attgctgacg
      301 ataaaaccta tcatgagaaa aaaataacta tgatggcagc catcattcaa attattataa
      361 ttgatttggt tttttcattc gattcaattt taacagcagt tggtttaagc agccaaatta
      421 tcattatgat attagctgtg gtagttagca tgtttgtaat gctgtttttt gctaaatatg
      481 taagcgactt tgtaaacaag tatcctaccg taaaaatgct tgcattatgt tttttagtag
      541 taataggagc tttacttgta atcgaatcgt tgcacattca tatcgaaggt attgacttta
      601 aaagctacgc ttatgtagcc atggcatttt cactcatagt tgagttgctt aatatcagat
      661 tgcgtaacgt gcttgataaa aaggcaaatg atgactaaac aaattgtttt gcccagcttt
      721 gtgcaacagg ctttagccac tcgtttttaa aagcgcctac tgtttcaatt aaattatcat
      781 acatctttgc attttcaggt gcattgctta ataaatggag tggcattaat ttaatttcat
      841 tatcggtttt aattgcaacc tgcattctat cgaaaaaatg tacattaaag gcttgctcga
      901 gttgttgaac aaaatgaacc gatttatcaa tagagcaacc gcttgcatcg ttcacctgtt
      961 gatttactgc aataactaaa aacaaattat ctacccaatt aaaactcgct tgtaaaaggt
     1021 tgttgtgggc tgtccagttt tcaataaaat caatggtttt cgaagtaagc aatttgcctt
     1081 ccgcttcgtt taaaaaacgg ttgcattgat atatccatat acgcgatgta tcaggtaatt
     1141 cattaaatga tatgtacata tttgtgggtg cttgattaac tacaaaagta tatataaccc
     1201 cctttaaatt ttgcagatta ttttactttt aatttaattg ttgccttaat tcttatttcc
     1261 attaaattta acctaatgaa gaaaactttt ttggctctca gccttttatc gctacatact
     1321 tatttgttgg cacaaaccga taataccaaa tcatcggttg gtacaaccag catggtttac
     1381 gcccattcaa aaaattgttt cgaagactat tacaaca
//
LOCUS       DAOMAA010000683         1415 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_110537_length_1415_cov_2.299265, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000683 DAOMAA010000000
VERSION     DAOMAA010000683.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110537_length_1415_cov_2.299265"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="PLO59_08815"
     CDS             <1..211
                     /locus_tag="PLO59_08815"
                     /inference="COORDINATES: protein motif:HMM:NF013024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uL15m family ribosomal protein"
                     /protein_id="HQV01248.1"
                     /translation="DAVQQLIDKHNITDITLEVLIEKGLVAKRVLVKILGRGEIKAKA
                     NITAHAFSATAKQAIESLGGTASTI"
     gene            219..>1415
                     /gene="secY"
                     /locus_tag="PLO59_08820"
     CDS             219..>1415
                     /gene="secY"
                     /locus_tag="PLO59_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HQV01249.1"
                     /translation="MKKLITTLQNIWKIEDLKSKITVTLICILVYRFGAHIVLPGVDP
                     QALGALKSQTEGGILGILDMFAGGAFSKASIFALGIMPYISASIVVQLLSMAVPRFQK
                     LQKEGESGRKKLSQYTRFLTVLVCLGQAPGYLVNLQSQAPGAIIATTHPELVSMFQFT
                     LTSVLIITAGTLFVMWLGERIQEKGIGNGTSLIIMVGIIARMPHAIYAEFVSRLGDTG
                     GAVVFLIEILIWLLVIAAVILLVQGTRKIPVQFAKRIEGNRQYGGVRQFIPLKVNTAG
                     VMPIIFAQAIMFIPATVAQFFPESAAWQGVASKFIDFSSFWYNFTFAFLIIIFTYFYT
                     AIAVNPNQMADDMKKNGGFIPGVKPGRKTADFIDDVMSRITLPGSLFLAFVAIMPALA
                     SIGGVNQ"
BASE COUNT          430 a          238 c          297 g          450 t
ORIGIN      
        1 agatgctgtg caacaattaa ttgataagca caatatcact gatattacct tagaagtatt
       61 aatagaaaaa ggattagtgg cgaaacgtgt tttggttaaa atattaggtc gcggtgagat
      121 taaagccaaa gccaatatta cagcacatgc attttcggct acagctaaac aagcaatcga
      181 atcattgggt ggtacggcct caactatcta aaaaataaat gaagaaactc ataactacat
      241 tacagaatat ctggaagatt gaagacctga agtcgaaaat cacagtaaca cttatttgta
      301 tactggttta tcgatttgga gctcatattg tattgccagg tgttgaccca caagctttag
      361 gcgctttaaa gagccaaacc gaagggggaa tattaggcat cttagacatg tttgctggtg
      421 gtgcttttag taaggcatct atttttgcat tgggaattat gccttacata tcagccagta
      481 ttgtagtaca attacttagt atggctgtac ctcgttttca aaagcttcaa aaagaaggcg
      541 aaagcggacg caaaaagtta agccaataca ctagattttt aaccgtttta gtttgtttag
      601 gccaagcccc gggatattta gtaaatctgc aatcgcaggc tccgggcgcc attattgcca
      661 caacacatcc ggagttggta agtatgtttc agtttacgct tacttcagta ttaattatta
      721 cagcaggaac attatttgtt atgtggttgg gcgaacgaat tcaagaaaaa ggaattggaa
      781 acggtacctc attaattatt atggttggta ttattgcgcg tatgccacat gcaatttatg
      841 ctgagtttgt atcacgttta ggagatactg gtggtgctgt agtattttta atagaaatat
      901 taatatggtt attggtaata gcagccgtta tacttttagt acaaggtact cgtaaaattc
      961 cggtacaatt tgcaaaacgt atagaaggta accgtcaata cggtggtgtg cgtcagttta
     1021 ttccattaaa agtaaataca gcaggcgtaa tgcccattat ttttgcacaa gcaattatgt
     1081 ttattcctgc tacggtggcc cagtttttcc ctgaaagtgc tgcttggcaa ggagttgcat
     1141 caaagtttat tgactttagt tcattttggt acaactttac ttttgctttc ttgattatca
     1201 tatttaccta tttctataca gccattgccg taaatccaaa tcaaatggct gatgatatga
     1261 agaaaaatgg tggctttatt ccaggtgtga aaccaggacg taaaacggca gattttattg
     1321 atgacgtaat gtcgcgtatt actttgccag gctcattgtt tttagcattt gtagccatta
     1381 tgccagcatt ggccagcatt ggtggcgtaa atcaa
//
LOCUS       DAOMAA010000684         1413 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_110852_length_1413_cov_2.272459, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000684 DAOMAA010000000
VERSION     DAOMAA010000684.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110852_length_1413_cov_2.272459"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..124)
                     /locus_tag="PLO59_08825"
     CDS             complement(<1..124)
                     /locus_tag="PLO59_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005831803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HQV01250.1"
                     /translation="MRIDKWLWAVRIFKSRSLAAEACNKGKVTVNEQVVKPARTI"
     gene            1096..>1413
                     /locus_tag="PLO59_08830"
     CDS             1096..>1413
                     /locus_tag="PLO59_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008990603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HQV01251.1"
                     /translation="MKCGIVGLPNVGKSTLFNCLSNAKAQAANFPFCTIEPNLGVITV
                     PDERLQALEAIVNPQKVVPTTVEIVDIAGLVKGASKGEGLGNQFLANIRECDAILHVL
                     RCFD"
BASE COUNT          454 a          237 c          259 g          463 t
ORIGIN      
        1 taattgtacg agccggctta acaacctgct cattaacggt aactttaccc ttattacatg
       61 cttcggcagc taacgacctg cttttaaaaa tcctgactgc ccaaagccac ttatctatcc
      121 gcataattaa gttccatttt tataaacttc aattttatgt attcatataa agtcgcacaa
      181 atgtagttgt aaagcacaac ataattaagc tttcatttat ttttgaagtg ttgcttctga
      241 atggtgtttt atgaaactat atctcaaact gcaactatta caattgtagg caaataaaac
      301 ggctggtgca cttgcctgtt gcatgcagta acaccagatg gaagtataaa gcggtaagta
      361 gtattgccat ttgcacaact tgtaaacatt agctggtttt ttcaaaacaa cgtttccgta
      421 taaaaacact tttattctat cgggcgaaat ccgggaataa gtgtcagcat ctgtaacatc
      481 attaatttcg ttttatgccc gtatcgaatc gttggtaatt gttggccgcc tgattcatag
      541 ttcatgaaac ggtctttatt gcaaagcagt atttttaaag gtgctcgcaa tacaacctcc
      601 ttaattaaaa gaaagcttcg ctaaactttt ttgagaataa ccgtttacag aagtaatgca
      661 tttattataa atatacatat ttttaaatgg aactttaagt taggattctg caattattta
      721 acctaaatcg ccataacgaa gtttacaata gtaatgggct ttgtaaaaca ccggcacgtt
      781 taggcacagc atgaggcttg aggcaagtgg tatgtggtat gtggtataga ttggaatgtg
      841 aacattgaaa ataaaattaa ttgttagatt gaagatatca atttgtttgc tgcttccaaa
      901 aacttgaaaa agttatttta taaaaaaact aacaatatgt tacttagtta atcatattca
      961 atttaaaatt tataaaacaa aatgtttcct ttgccgcccg aaaaatgggt agttttaagt
     1021 aggagctaca cgtcaatata aaggcgtgat ttttatcttt tatccccttt tttcataacg
     1081 caaaaaataa taattatgaa gtgtggtatt gtaggtttac ccaatgtagg taaatcaacc
     1141 ttatttaatt gcttaagcaa tgccaaagcg caggcagcta attttccgtt ttgtaccatt
     1201 gaacccaatt taggggtaat aactgtaccc gatgagcgtt tgcaagcgtt agaggcaata
     1261 gtaaatccgc aaaaggtagt gcccaccacg gttgaaattg tggatatagc tggcttggta
     1321 aaaggtgcaa gcaaaggcga gggtttaggt aatcagtttt tagccaacat acgcgagtgt
     1381 gatgccattt tacatgtact gcgttgcttt gat
//
LOCUS       DAOMAA010000685         1410 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_111229_length_1410_cov_3.714391, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000685 DAOMAA010000000
VERSION     DAOMAA010000685.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1410
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111229_length_1410_cov_3.714391"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="PLO59_08835"
     CDS             complement(<1..219)
                     /locus_tag="PLO59_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010609468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQV01252.1"
                     /translation="MQLSFFQVTLLVADYDDAINFYVNKLGFLLLEDTKLSDTKRWVR
                     VAPWGNNTAALLLAKAGNENQLNFIGNQT"
     gene            complement(221..658)
                     /gene="msrB"
                     /locus_tag="PLO59_08840"
     CDS             complement(221..658)
                     /gene="msrB"
                     /locus_tag="PLO59_08840"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003005831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HQV01253.1"
                     /translation="METKNPYYSHTDTNTLDVSNDEWKKVLPDDVYQIMRLKQTERAF
                     TGKYWETETQGNYYCASCGNHLFKSTSKFSSSCGWPSFFEQLNSKSVVFERDTSYGMI
                     REEALCGRCGGHLGHRFDDGPPPTNMRYCMNSIALDFVPDTVK"
     gene            complement(832..>1410)
                     /locus_tag="PLO59_08845"
     CDS             complement(832..>1410)
                     /locus_tag="PLO59_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018343367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQV01254.1"
                     /translation="KEIKERHDFILKEQEDLANAKASLLQTILEIDQTAHEKFMDAFV
                     KVRENFILVFRSLFREEDKVDLLLADPTNPLESDINIIAQPKGKRPLSIQQLSGGEKT
                     LTATALLFGIYLLKPAPFCIFDEVDAPLDDNNIDKFIQIIKKFSNETQFIVITHNKKT
                     MTATDVIYGITMVEQGITQVVPVDMKQFAEAG"
BASE COUNT          396 a          261 c          261 g          492 t
ORIGIN      
        1 ggtttgattg ccaataaaat ttaattggtt ttcgttaccg gcctttgcca ataataatgc
       61 agccgtatta ttgccccaag gtgccactcg aacccaccgt ttggtatcag atagttttgt
      121 atcttctaac aacaaaaaac ccaatttgtt tacgtaaaaa tttatggcat catcataatc
      181 ggcaaccaat aatgttacct gaaaaaaaga taattgcatt ttattttact gtatcaggta
      241 caaaatctaa agcaatactg ttcatgcaat agcgcatatt ggtaggcggg gggccatcgt
      301 caaaccgatg gcctaaatgg ccaccacagc ggccgcataa tgcttcttcg cgtatcatac
      361 cgtaagaagt atcgcgttca aacaccacgc ttttcgaatt taattgttca aaaaaactgg
      421 gccaaccgca actgctgcta aatttactgg tgcttttaaa taagtgattg ccacacgatg
      481 cacaatagta attgccttgt gtttcagttt cccaatactt accggtaaag gcacgttccg
      541 tttgttttaa acgcattatc tgataaacat catcaggcaa aacttttttc cattcatcgt
      601 tacttacatc gagggtattt gtatcggtat gcgagtaata aggatttttt gtttccatgg
      661 tttgttttgt tgtagtaacc gtttgtgcag tttggctgtt gctgttactg cattgtgtaa
      721 gtagtaatat taatacgctg catactgcta tcagtttcat gatataatta atttttgtat
      781 ttattatacg ttgctgtaca gtatatggat taatttttct gctaaaaatg attagccggc
      841 ttcggcaaat tgtttcatat caaccggcac cacttgtgta ataccttgtt caaccatggt
      901 tatgccataa attacatcgg tggctgtcat ggttttttta ttatgtgtga taacaataaa
      961 ctgtgtttcg ttgctgaatt ttttaataat ctgaataaat ttatcaatat tattatcatc
     1021 cagcggggca tctacttcat caaaaataca aaacggtgca ggctttagca ggtaaatacc
     1081 aaataagagg gcagtggcag ttaatgtttt ttcgccacca cttaactgtt gtatcgaaag
     1141 ggggcgtttg cccttgggtt gtgcaataat atttatatcg ctttcgagtg ggttggttgg
     1201 gtcggccaat aataaatcta ctttatcttc ttccctaaac aagcttctga aaaccaggat
     1261 aaagttttcg cgcaccttca caaaagcatc cataaatttt tcatgtgcgg tttggtcaat
     1321 ttctaatatg gtttgtaaaa gcgatgcttt ggcattggca aggtcttctt gttcttttaa
     1381 aataaaatca tgtcgttctt ttatttcttt
//
LOCUS       DAOMAA010000686         1409 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_111404_length_1409_cov_2.813146, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000686 DAOMAA010000000
VERSION     DAOMAA010000686.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111404_length_1409_cov_2.813146"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="PLO59_08850"
     CDS             complement(<1..706)
                     /locus_tag="PLO59_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008245808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HQV01255.1"
                     /translation="MDIEKDELVEIYGAREHNLKNIDVQFPRNKLVVITGLSGSGKSS
                     LAFDTIYAEGQRRYMESFSAYARQFLGTMERPDVDKIAGLSPVISIEQKTTSQNPRST
                     VGTITEVYDFMRLLFARTATAYSYVSGQPMVKMDEVQIQNHILDSFNACTINILAPVV
                     KGRKGHYRELFEQLAGNGYMRVRIDGKFEELRKGLQADRYKVHDIEVVVDKLTVNELN
                     KQRLLASLRTALKTGKG"
     gene            837..>1409
                     /locus_tag="PLO59_08855"
     CDS             837..>1409
                     /locus_tag="PLO59_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01256.1"
                     /translation="MHNIDSFAKYLQQQFNKLGIRIFCFLFIINFKGLFAQCTNPIQT
                     FPFVADFEAGNGGWITGGNFSDWTWGTPTKAKISSAASGNNCWITGGLNTSFYNLNQR
                     SYLQSPCFDFTGIALPLISFNIYWETENQYDGCNLQYSLDLGNTWQNVGTANGLNNCT
                     NSNWYNNASINNLNGLATVRNGWCGNTLPTT"
BASE COUNT          434 a          286 c          239 g          450 t
ORIGIN      
        1 atcccttacc ggtttttaaa gctgtgcgta acgaggccag taaacgttgt ttgtttaatt
       61 cattaaccgt tagcttatct actactactt caatgtcgtg cactttatat ctatcagctt
      121 gcaacccttt acgcaactct tcaaatttac catcaatacg tacacgcata taaccattgc
      181 cggccaactg ctcaaacaac tcacgataat gacctttgcg tccttttaca acgggtgcca
      241 aaatgtttat ggtacatgcg ttaaaactat caagaatgtg attttgaatt tgcacttcat
      301 ccattttaac catgggctgc ccggatacat aactataagc agtagccgta cgtgcaaata
      361 acaaacgcat aaaatcatac acttcggtaa tggtgccaac ggtgcttcgg gggttttggc
      421 tggttgtttt ttgttcgata gaaattaccg gacttaaacc tgctatttta tccacatcgg
      481 gtcgttccat tgtgcctaaa aactgacgcg cataagccga aaaactttcc atatacctgc
      541 gttgtccttc ggcataaatt gtatcaaagg caaggctcga tttgccacta ccgctaaggc
      601 cggtaataac aactaactta ttgcgcggaa actgaacatc tatatttttc aaattatgct
      661 cacgtgcacc atatatttct accaactcat ccttttcaat atccataaat attcatactt
      721 ttaatacagc aatagtaagg tttacacaac taatgtcatt attataactg tgcaaaaact
      781 acttaatatt tgcttaacaa tttctattta aacatcgcat taaacataca ctgccattgc
      841 ataacattga tagttttgca aaatatttac aacaacaatt taataaattg ggcatacgta
      901 tattctgctt tttatttata ataaatttta agggtctttt tgcacaatgc acaaatccga
      961 tacaaacttt tccgtttgta gcagattttg aagcaggtaa tggtggctgg attaccggtg
     1021 gtaattttag cgattggaca tggggcacgc ctaccaaagc aaaaataagt agcgctgcta
     1081 gtggtaataa ttgttggatt accggaggac taaacacctc gttttacaat ttaaatcaac
     1141 gcagttattt acaaagtcct tgttttgatt tcactggtat agctttacca cttataagtt
     1201 ttaatattta ttgggaaacc gagaatcaat atgacggatg taacctgcag tattcgctcg
     1261 atttaggtaa tacatggcaa aatgtaggca ctgccaatgg ccttaataat tgtaccaatt
     1321 caaactggta taataacgct tcgataaata atttaaatgg attggccacg gtgcgaaatg
     1381 gctggtgtgg caatactttg cctacaacc
//
LOCUS       DAOMAA010000687         1408 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_111558_length_1408_cov_2.991131, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000687 DAOMAA010000000
VERSION     DAOMAA010000687.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111558_length_1408_cov_2.991131"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="PLO59_08860"
     CDS             <1..202
                     /locus_tag="PLO59_08860"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010517299.1"
                     /note="catalyzes the formation of
                     (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-
                     carboxamido)succinate from
                     5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate and
                     L-aspartate in purine biosynthesis; SAICAR synthase;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HQV01257.1"
                     /translation="QWLIQNGFQGLEGQQVPLMTDEIVNEISNRYIELYESITGLQFV
                     KADNTNMQLRIESAVNKYLACL"
     gene            444..773
                     /locus_tag="PLO59_08865"
     CDS             444..773
                     /locus_tag="PLO59_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01258.1"
                     /translation="MKKLILIACFFIGYTAQAQTVLFNGTPVMGDTVYIKKNSNNYLS
                     IDYVKPTHGIYQFQALMNAEPISCGSQNGTLHVNEKGRYKMRMIWYHDNTAEIFFKKL
                     IYVKYIN"
     gene            complement(876..1352)
                     /locus_tag="PLO59_08870"
     CDS             complement(876..1352)
                     /locus_tag="PLO59_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01259.1"
                     /translation="MLLIQNNADALNLKHAIKNVCVEIVLQQIFILKQTINDLQLAAN
                     NESKSTAGDKHDTSKAILQQQVAMHHQQLIVAQNQSKQAEALPVAINDLPTLGSLIYA
                     NEQCFYIGMGIGKKTINNTEIILLAPTAPLAQFFIGLKNKTEGVFNSKTYTIKQCW"
BASE COUNT          476 a          221 c          233 g          478 t
ORIGIN      
        1 tcaatggctt attcaaaatg gatttcaagg attggaaggg caacaagtac ctttaatgac
       61 tgatgaaatt gtgaacgaaa taagtaaccg ttatatcgaa ttatacgagt caattacagg
      121 tttgcaattt gtaaaagctg ataacaccaa tatgcagtta cgtattgaaa gtgctgtaaa
      181 taaatattta gcctgcttgt aaattgtttt attaattgtt gggttaattg ttgatttttt
      241 aaagcgtaca gcagtttttt tatggctaaa aattattcat aaaatacgaa cacttttatc
      301 aacaaagcca ataaaattgg ctgttttcag ccgcttagta aattagatat ttaaccgttt
      361 ttaaatacgt gtttgccaaa taaatgtaat ggttaataag cgtaaaaata tttttgacgc
      421 acctaaacaa cccaacattt attatgaaaa aacttatttt aattgcctgc ttctttattg
      481 gatatactgc acaggcgcaa accgttttat ttaatggaac gcctgttatg ggcgataccg
      541 tttacatcaa aaaaaacagc aacaattatt tgtcaattga ctacgtaaaa ccaacccatg
      601 gtatttatca gtttcaggca ttaatgaatg ctgaaccaat ttcatgcggc agtcaaaatg
      661 gtaccttgca tgtaaatgaa aaaggccgct ataaaatgcg tatgatttgg taccatgata
      721 acacagcaga aatatttttt aaaaaattaa tctacgtaaa gtacatcaac taaaatcaat
      781 ttccattaca aaccaaaaga aagctgccaa accacaggta gcttttttta tatctgatta
      841 aaaattaaag tgctttatta aaacaacaac accggctacc agcattgttt tatagtatag
      901 gttttagaat tgaaaacacc ttcggtttta tttttaagcc ctataaaaaa ttgtgctaat
      961 ggtgccgtgg gagctaataa aataatttca gtgttgttga ttgttttttt gccaataccc
     1021 atgccaatat aaaagcattg ctcatttgca taaattaggc tgcctaatgt cggcaagtca
     1081 tttatggcaa caggcaaggc ctccgcttgt ttactttgat tttgagctac tatcagttgc
     1141 tgatgatgca tggctacctg ttgttgcaaa atggcttttg aagtatcatg tttatcgcct
     1201 gctgtacttt tgctttcatt attggctgca agctgtaaat cgtttatagt ttgttttaaa
     1261 ataaaaatct gttgcaatac gatttctaca catacatttt taatggcatg ctttaaattt
     1321 aatgcatcag cattgttttg aattaacagc aattgtataa acgatattga atattaaaag
     1381 cttaaaaagc aaaaatggcg tgcctggt
//
LOCUS       DAOMAA010000688         1408 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_111584_length_1408_cov_2.699187, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000688 DAOMAA010000000
VERSION     DAOMAA010000688.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111584_length_1408_cov_2.699187"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="PLO59_08875"
     CDS             <1..225
                     /locus_tag="PLO59_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01260.1"
                     /translation="YMVVKDQFYRGIYLKTNDLIRAEFVDDALAQKAAKMLVQYSSNK
                     PQTVALFHLNENLITTYEISDFKKLFDTYR"
     gene            182..>1408
                     /locus_tag="PLO59_08880"
     CDS             182..>1408
                     /locus_tag="PLO59_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01261.1"
                     /translation="MKFPILKSCSILIVSAAIVFIGYKNNACSFFYDCDEWHYAFFNP
                     EVIQQPHVESFFVTCHGLHDNNFIYSITDSSMAEWQQYFNNRLELDQLKQLFQLNQNE
                     TKQLAQSIKQNTRVNQSLSKLFTQLAATKNTAAIDYFLWAKKTEPLVQAKADYWSYEP
                     TVIDTAVYLHNIADLKALTENTSDVFLKQRYAFQVLRTLFYLDDYQACIAFYNTKFSS
                     IKINNSITARALSYYAGCYYRLKDYATSNYLFSKIYRQHPALKESAFLSFHPWSDSDW
                     NATLAMAKSNTEKETLWHLFGIYADPLKGMQEILKINAKSNFADLLLVRAINILERDK
                     LINNHEGLFENEFYNDESSEIKIFDSSVSFRAFKQDTATDTLIKFVNNQALQNKSAVW
                     INAAAYLNWLQKDNKTA"
BASE COUNT          496 a          249 c          240 g          423 t
ORIGIN      
        1 tacatggttg taaaagatca attttaccgg ggcatatatt taaagaccaa tgatttaatc
       61 cgtgccgaat ttgttgatga tgctttggca caaaaggcgg ccaaaatgct tgtgcagtat
      121 tcatccaata agccgcaaac agtagcatta tttcatttaa acgaaaactt aataaccacc
      181 tatgaaattt ccgattttaa aaagttgttc gatacttatc gttagtgctg ccattgtttt
      241 tatagggtat aaaaataatg cttgctcctt tttttatgat tgcgatgaat ggcattatgc
      301 attctttaat cccgaagtga tacaacaacc ccatgttgag tcgttttttg ttacctgcca
      361 cggtttgcat gataataatt ttatatacag cattaccgat agcagtatgg ccgaatggca
      421 acaatatttt aataaccggt tagaactgga tcaattaaaa caactgttcc agctaaacca
      481 aaacgaaaca aaacaattag cgcaaagcat aaaacaaaac acccgtgtta atcagtcgct
      541 ttcaaaactt ttcacgcagt tagcagctac aaaaaataca gcagctattg attacttttt
      601 atgggcaaaa aaaacagaac ctttagtaca agctaaagct gattactgga gctatgaacc
      661 cacagttatt gacacagcag tttatctgca caacatcgct gatttaaaag cgctaacaga
      721 gaacacttcc gatgtttttt tgaaacaacg ttatgcattt caggtgctgc gcacgctttt
      781 ttatttagac gattatcaag catgtattgc cttttataac acaaaatttt catctataaa
      841 aataaacaac agcattacag cccgtgcttt atcatactat gcaggttgct attaccgctt
      901 aaaagattat gcaacttcaa attatttgtt ttcgaaaata tacaggcaac atcccgcatt
      961 aaaagaaagc gcttttttaa gttttcatcc ctggagcgat agcgactgga atgcaacgct
     1021 ggcaatggca aaaagcaata cagaaaaaga aacattgtgg catttgtttg gaatttatgc
     1081 cgacccgcta aaaggaatgc aggaaatttt aaaaatcaat gccaaatcaa attttgccga
     1141 tttactttta gtaagagcta taaatatttt agaacgggat aaactgataa acaaccatga
     1201 aggcttattt gaaaacgagt tttataatga tgaaagcagt gaaattaaaa tttttgattc
     1261 atcggtatcg tttcgtgctt ttaaacagga taccgcaacc gatacattga taaagtttgt
     1321 aaacaatcaa gcattgcaga ataaaagtgc agtgtggata aatgctgctg catatttaaa
     1381 ttggttacaa aaggataata aaacagca
//
LOCUS       DAOMAA010000689         1407 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_111721_length_1407_cov_2.820266, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000689 DAOMAA010000000
VERSION     DAOMAA010000689.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111721_length_1407_cov_2.820266"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="PLO59_08885"
     CDS             <1..572
                     /locus_tag="PLO59_08885"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010420070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HQV01262.1"
                     /translation="MQDTFFISDTMALRTHTSSVQVRVMENKQPPIRTISPGRVYRNE
                     AISARAHCFFHQVEGLYIDEGVSFADMKQTLLHFAQEMFGAQSNIRLRASYFPFTEPS
                     AEMDVTCFICNGKGCNICKGSGWVEILGCGMVDPAVLTNCGIDAEKYTGFAFGMGIER
                     ITMLKYQIKDLRIFSENNVRFLQQFSGMN"
     gene            606..983
                     /locus_tag="PLO59_08890"
     CDS             606..983
                     /locus_tag="PLO59_08890"
                     /inference="COORDINATES: protein motif:HMM:NF017935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HQV01263.1"
                     /translation="MPTILSSFILCKCPQCRQGNMFKPRGPIPFWDFGIMHDRCAVCD
                     LNFKPEPGFYFGASILSYGYGVFVMGLTWFIMWYLHLDETWLLLTTITLVLVLFSPYN
                     FKMSRAGWLAFTVAYKPNLKKKI"
     gene            1010..>1407
                     /locus_tag="PLO59_08895"
     CDS             1010..>1407
                     /locus_tag="PLO59_08895"
                     /inference="COORDINATES: protein motif:HMM:NF039704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6252 family protein"
                     /protein_id="HQV01264.1"
                     /translation="MKKKLVLFLVIAMGFMGCNKDSETPIKYFLKANIYGATWEANTS
                     LFADQAIVLTVNGMKSDGSTLTMEINDVTPGTYPITNDRNHILYTSQLADAFESKNAL
                     PGSLVIISNDVSKNIIKGTFQVTVQSPLTG"
BASE COUNT          425 a          261 c          288 g          433 t
ORIGIN      
        1 acatgcagga tacttttttt atttccgaca caatggcgct tcgtacacat acatcaagcg
       61 tgcaagtacg tgtaatggaa aataaacaac caccaatccg aaccatatca cccggccgcg
      121 tttaccgcaa cgaagccatt agcgcaaggg cacattgttt ttttcatcag gtagaaggat
      181 tgtatattga tgaaggcgtt tcgtttgccg atatgaaaca aaccttgctg cactttgcac
      241 aagaaatgtt tggtgcgcaa tcaaatatca ggttaagggc atcatacttc ccctttaccg
      301 agcccagtgc cgaaatggat gtaacctgtt ttatttgtaa cggtaagggt tgcaacattt
      361 gtaaaggcag tggctgggtc gaaattttgg gctgcggtat ggttgaccct gcagtgctta
      421 ccaactgcgg cattgatgcc gaaaaatata caggctttgc ttttgggatg ggcattgagc
      481 gcataaccat gctcaaatat cagattaaag atttgcgtat tttctctgaa aacaatgtac
      541 ggtttttaca acagtttagt ggtatgaatt aatatagttt tttaaaactt aaattataaa
      601 attttatgcc aacaatactg tcttcattta ttttatgcaa atgtccgcaa tgccgccaag
      661 gtaacatgtt taagcccaga ggcccaattc ctttttggga ttttggaatt atgcatgacc
      721 gatgtgccgt ttgtgattta aattttaaac ccgaacccgg tttttatttt ggcgcatcaa
      781 ttcttagcta cggttatggt gtttttgtaa tgggtttaac atggtttata atgtggtatt
      841 tgcatttgga cgaaacctgg ctattgctta ccaccattac tttggtttta gttttgtttt
      901 cgccatataa ttttaaaatg agccgtgcag gctggttggc atttacggtt gcttacaagc
      961 caaacctgaa aaagaaaatc tgaagaattc tgaaaataaa tataattcaa tgaaaaaaaa
     1021 attagtatta tttctggtaa ttgcaatggg ttttatgggt tgcaataaag actcggaaac
     1081 acccattaaa tattttttaa aagcaaacat ttatggtgca acctgggagg ccaacaccag
     1141 cctatttgcc gatcaggcca tagtgcttac cgttaacgga atgaaaagtg atggcagcac
     1201 attaacgatg gagataaacg atgttacgcc cggcacctat ccaataacca acgatagaaa
     1261 tcatatttta tacaccagcc agttagccga tgcttttgaa agtaaaaatg cattgccggg
     1321 cagccttgta attataagca atgatgtttc gaaaaatatt attaaaggca catttcaggt
     1381 tacggtgcaa agtccactta ccggcaa
//
LOCUS       DAOMAA010000690         1407 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_111779_length_1407_cov_2.268491, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000690 DAOMAA010000000
VERSION     DAOMAA010000690.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111779_length_1407_cov_2.268491"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="PLO59_08900"
     CDS             complement(<1..234)
                     /locus_tag="PLO59_08900"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQV01265.1"
                     /translation="MILLTGGTGLLGTHTLLALARKNIAVVAIKRPASDLQTFYYLAE
                     KAGVNLKHITWFDADLLDVNAIYEVTKNINTVIH"
     gene            384..>1407
                     /gene="tyrS"
                     /locus_tag="PLO59_08905"
     CDS             384..>1407
                     /gene="tyrS"
                     /locus_tag="PLO59_08905"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002664363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HQV01266.1"
                     /translation="MKNFVDELKWRGMLQDIMPGTEELLNSKRVIGYIGFDPTADSLG
                     VGNLVQVMTLLHFQRCGHTPMILLGGATGMVGDPSGKSAERNLLNEDVLQKNLAGQKR
                     QLEKFLAFEFVENPALIVNNFDWFKSFGFLDFIRDVGKHITVNYMMAKDSVKSRLENG
                     MSFTEFSYQLVQGYDFYHLWKNNQVQLQMGGSDQWGNIVTGTELIRRKDGGEAFALTT
                     PLIRKADGTKFGKTEGGNIWLDAQRTSPYKFYQFWLNTSDEDAKNYIRIFTIKTKEEI
                     EALTAEHDVNPSLRVLQKALAQEVTIRVHSLQDYEAAVEASEILFGKATTETLKNIDE
                     QMFLQVF"
BASE COUNT          445 a          255 c          292 g          415 t
ORIGIN      
        1 atgtattacc gtatttatat tttttgttac ctcataaatg gcatttacat ccagcaagtc
       61 ggcatcaaac catgtaatat gcttcaaatt aaccccggct ttttcggcca aataataaaa
      121 cgtttgtaaa tcagatgcag gccgtttaat agcaactacc gctatgtttt tacgtgccag
      181 tgccaataaa gtatgcgtgc ctaataatcc tgtgcccccc gttaaaagta tcataccgct
      241 ttacgtgcta ttaaataaat gttaggctct atacacatag ttaaacaagt gtgggcaaac
      301 atagttaact tattacattc gccacccgta aatattttta ggttgcagat tataaccttc
      361 atttttaaac aggacaaatt caaatgaaaa attttgttga tgaattaaag tggcgcggca
      421 tgttgcaaga tataatgccc ggtaccgaag aattgttgaa cagcaaacgt gttattggtt
      481 acataggctt tgaccccact gccgactcgt taggtgtggg caatttggta caggttatga
      541 ctttattgca ttttcaacgt tgcgggcata cacctatgat tttattgggt ggtgcaaccg
      601 gtatggtggg cgatccatca ggcaaatcgg ccgaaagaaa tttgctgaac gaagacgtgt
      661 tgcaaaaaaa tttagccgga caaaaaaggc aactcgaaaa atttttagca ttcgaatttg
      721 tcgaaaatcc ggccctgatt gtaaataatt ttgattggtt taagtctttc ggatttttag
      781 attttatcag agacgtaggc aagcatatta ccgttaatta tatgatggct aaggactcgg
      841 taaaatcgcg cctcgaaaac ggtatgtcgt ttaccgaatt tagttatcag ttagtgcaag
      901 gttatgattt ttaccactta tggaaaaata atcaggtgca gttgcaaatg ggtggcagcg
      961 atcagtgggg taatatagtt accggcacag agttgattag acgtaaagat ggtggcgaag
     1021 cttttgccct tactacacct ttgataagaa aagctgatgg aaccaaattt ggtaaaaccg
     1081 aaggtggcaa tatctggtta gatgcccaac gtacttcgcc ttataaattt tatcagtttt
     1141 ggcttaatac cagtgacgaa gatgctaaaa actatatacg gatttttacc ataaaaacca
     1201 aagaagaaat agaagcgcta accgcagaac atgatgtaaa tccatcgttg cgtgtgttgc
     1261 aaaaagcatt ggcacaagag gtaaccattc gtgtacactc cctgcaagat tacgaagccg
     1321 cagttgaagc cagcgaaata ttatttggca aagccactac cgaaacctta aaaaatattg
     1381 atgaacaaat gtttcttcag gtgtttg
//
LOCUS       DAOMAA010000691         1406 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_111893_length_1406_cov_2.977794, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000691 DAOMAA010000000
VERSION     DAOMAA010000691.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111893_length_1406_cov_2.977794"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="PLO59_08910"
     CDS             <1..323
                     /locus_tag="PLO59_08910"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HQV01267.1"
                     /translation="LQWLAIILSPYAPHIAEEVWQLMGNTQSVTKAAWPVFNESYLIE
                     SVFEYPVSINGKMKFKFPLPTNYTSAQVTENISISPKLQQLLQGATPKKIIVVPGKII
                     NVVV"
     gene            356..913
                     /gene="scpB"
                     /locus_tag="PLO59_08915"
                     /pseudo
     CDS             356..913
                     /gene="scpB"
                     /locus_tag="PLO59_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014019326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
     gene            1131..>1406
                     /locus_tag="PLO59_08920"
     CDS             1131..>1406
                     /locus_tag="PLO59_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008146679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HQV01268.1"
                     /translation="MLSRTAHIDSLLKTLPQNPGVYQYFNKEGSIIYVGKAKNLKKRV
                     MSYFSKDTFDSNKTLMLVKSIADIKLIITETEHDALLLESNLIKKHQP"
BASE COUNT          494 a          285 c          251 g          376 t
ORIGIN      
        1 ttttgcaatg gctggccatt attttatcgc cttacgcacc acacattgcc gaagaagttt
       61 ggcaactgat gggcaacaca caaagtgtaa ctaaagcagc atggcctgtt tttaacgaaa
      121 gctatttgat agaaagtgta ttcgaatacc cggttagcat taacggaaaa atgaaattca
      181 aatttccgtt accaactaat tacacatcgg cacaagtaac cgaaaacatc agcattagcc
      241 ctaaactaca acaactgctt cagggtgcaa cacctaaaaa aataattgtt gtaccgggca
      301 aaattatcaa cgtagtagtt taacctttct gctttcaacc taattttaat ttataatgga
      361 acatttacca caaaatattg aagccttaat ttttgcaagc gaaaacagta ttaccattaa
      421 cgaaatacaa cattgcctta aaactgttta tggttgggag ctggctattg aagacatacg
      481 tgcttgtatt gatgacttac agcaaaaata taacgacccg cagttttcat ttgcattagc
      541 cgaaattgcc gaaggtttca ggtttttaac caagcccgag ttttatggcg ctatccaaat
      601 atttatccag caaaaaaata aaaaacggct tagcacagca gcactcgaaa cactaagcat
      661 tattgcctac aggcagcctg tttcaaaagc tgagttagaa catgtgcgtg gcgtaagttg
      721 cgattacagt attcaaaaac ttttagaaaa agaattgatt gaaataacag gcaagagcga
      781 tgcgcccggc aaaccaattt tatatggcac cagtaaattg tttatggatt actttggcct
      841 taaagacccc aaagacttac ccaagcttaa agatctgcaa gccattgata atgttattgg
      901 cacatcattc gaaagtatag acagcgaatt gcccgaaggc attacgcttg aagcagcaag
      961 cgatgcaaca gatacggatg accataccga taccacacaa accaacaatg atagtggtgc
     1021 cgacccaaca ccacccatta gctgataatg cttgaagtgg ttttattttt aacaaatcat
     1081 ttttattgta ccaatccgtt acactgcacg gagtttattg ctgcataaaa ttgttaagtc
     1141 gcactgcaca tattgattca ttgttaaaaa cactgccgca aaatcccggt gtttatcagt
     1201 actttaataa agaaggcagc atcatttatg ttggcaaggc aaagaattta aaaaaacgtg
     1261 tgatgagcta ttttagcaaa gatacgtttg acagcaacaa aacattgatg ctggttaaaa
     1321 gcatagccga catcaaatta attataaccg aaaccgaaca cgatgcattg ttgctcgaaa
     1381 gcaacctaat aaaaaaacac cagcca
//
LOCUS       DAOMAA010000692         1405 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_112089_length_1405_cov_2.181481, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000692 DAOMAA010000000
VERSION     DAOMAA010000692.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112089_length_1405_cov_2.181481"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            51..>1405
                     /locus_tag="PLO59_08925"
     CDS             51..>1405
                     /locus_tag="PLO59_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01269.1"
                     /translation="MAAGSFTHAQTACNNCPGTVYTTVNSSTMGNLLTNAKFCITNQL
                     IVDADLTLDKCILLMSAGASIVITGSNKLKITNNTYITSCGYGNGTMWQGIHVTSVNL
                     NNFSKLEISNGAIIEDAITAVYTESQDAYCEIKLDNAHFNRNYYSVFIDRANPALLPL
                     VTRCGWLGFIDRCTFTCNSTYNSGINDLLQPPNNTMRSKTHVEIRCAQRFNNIPNGLI
                     PITNSVFDNSNTGVFSFYSETMLNSNTFNNHNMAVDAYFDGALTMFDNTISNVSFGID
                     VHDAVHHVELLQNTITGPAMRGISVRKSCSGPNDVSFNTITDCSYGIRIIGNGISNTA
                     GYNFRLHQNNISNSSGAAFGIDFIETPNFSPAQTNLYFVDITENEIAGVAVGIRLTNG
                     YKAKIADNNVAIIDNTGTLGIGAINSYMPHVIANNVAGPSLNGQCENTGLNTTGILIS
                     NT"
BASE COUNT          442 a          286 c          258 g          419 t
ORIGIN      
        1 caacaaaaat cagcagttgt gcaagtaaaa tcttatttat tttaatgctg atggctgccg
       61 gcagttttac acatgcacaa acggcctgca acaattgccc gggcactgtt tacacaaccg
      121 ttaactcaag taccatgggc aatttactaa ctaatgcaaa gttttgcatt accaatcaat
      181 tgattgttga tgccgattta acactcgaca aatgcatctt acttatgagt gcaggggcat
      241 ctattgttat tacaggcagc aacaaattaa aaattaccaa taacacctat ataacatcgt
      301 gtggttacgg taatggcact atgtggcaag gtattcatgt tacaagcgtt aatctcaata
      361 atttttcaaa acttgaaata agcaacggtg ccattatcga agatgcaatt acggctgtat
      421 atacagaaag tcaggatgca tactgcgaaa taaaattaga caatgcacac tttaatcgta
      481 actactattc tgtttttatt gacagggcca accctgcact tttaccattg gtaacacgtt
      541 gtggttggtt aggttttatt gaccggtgca catttacttg caattcaact tacaactcag
      601 gtattaatga tttgctgcaa ccaccaaata atacaatgcg ctctaaaacc catgtcgaaa
      661 tacgttgtgc ccagcgcttt aataatattc caaatggttt aatacctata accaattctg
      721 ttttcgataa cagcaataca ggcgttttca gcttctatag cgaaaccatg ttaaacagca
      781 atacttttaa taaccacaac atggctgtgg atgcttattt tgacggtgcg cttaccatgt
      841 ttgataatac catcagcaat gtttcattcg gtattgatgt gcacgatgct gtacaccatg
      901 ttgaattgtt gcaaaataca attaccggcc cggctatgcg aggcatttct gtgcgcaaat
      961 cttgtagtgg cccaaacgat gtttcattta acaccattac cgattgcagc tatggcattc
     1021 ggataattgg caatggaatt tcaaatacag ccggttataa ttttaggttg catcaaaaca
     1081 atattagcaa cagcagtggt gctgcctttg gtattgattt tattgagacc cctaatttta
     1141 gtcccgcaca aaccaatctt tattttgttg atattaccga aaacgaaatt gcaggagtgg
     1201 cagtgggcat acggcttacc aatggctaca aagcaaaaat tgctgataac aatgtagcca
     1261 tcattgacaa taccggtacc ttgggtattg gcgccataaa tagttacatg cctcatgtaa
     1321 ttgcaaacaa tgttgcaggc ccaagcctta atggtcaatg cgaaaataca ggcctcaata
     1381 caacaggtat tcttataagc aatac
//
LOCUS       DAOMAA010000693         1404 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_112195_length_1404_cov_2.656042, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000693 DAOMAA010000000
VERSION     DAOMAA010000693.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112195_length_1404_cov_2.656042"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="PLO59_08930"
     CDS             complement(<1..336)
                     /locus_tag="PLO59_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01270.1"
                     /translation="MKNANRITVAIYFVCITLCCFIKVSHAQPYHPSNGTLYNTAVVP
                     RIDIEIDPDSLALILNNPYSDNEYHATFTFNDGNIKDTVLNIGFRLRGNTSRASAKKA
                     FKVSFNTFEK"
     gene            complement(403..>1404)
                     /locus_tag="PLO59_08935"
     CDS             complement(403..>1404)
                     /locus_tag="PLO59_08935"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV01271.1"
                     /translation="QSCIPDPQFEIADDTICIKSSVQYFDKSQRFPNQWNWSFPGGTP
                     STSTLKNPVVTYNSTGNYSATLIATSPEGSDTVTINNVVKVVAQPNPKITANGATSFC
                     VGNVNLSTTYNSTYTYQWYKNGNIINGATDTAFNAINAGSFTVRATNKFGCTNESAAI
                     VLSTPLVAKLKNVGSSAVCPGDSVLLNASPVGTGLTYEWYRNNILLPTVTGSSYYAKV
                     GGVYKVRISESPTCINLSKAKILTDGCRETKTADNNLLAVYPNPSNYEFTIQPADEIT
                     ADYTISITDISGRLVMKETMINVSDVYTFGKDLPEGIYLLTLVANNKTETYRLVKTK"
BASE COUNT          414 a          255 c          258 g          477 t
ORIGIN      
        1 cttttcaaaa gtattaaacg aaaccttaaa cgcttttttt gcacttgcac gtgatgtatt
       61 gcctctaagt ctgaaaccta tatttaaaac tgtatcttta atgttgccat cattaaaagt
      121 aaatgttgca tgatactcat tatcactata agggttgttt aatataagtg ccagcgagtc
      181 ggggtcaatt tcaatatcaa tacgtggtac aacagcagta ttgtataaag ttccatttga
      241 tggatgatag ggttgcgcat gagatacttt tataaaacaa cacaacgtaa tacaaacaaa
      301 gtatatagct accgttatcc tatttgcatt tttcatattc gaatcaacct aaaaaaaccc
      361 atacaaactt gtatgggctt ttatcaaaaa ttatgttttc aattatttgg tttttaccag
      421 gcgatacgtt tcggttttgt tattggcaac aagcgtaagt aaatatattc cctcgggcaa
      481 gtctttacca aaagtataaa catcgctaac gttaatcatg gtttccttca ttactaaacg
      541 gccacttata tctgttatcg aaatagtata atcagctgta atttcatcgg ccggttgtat
      601 ggtaaattca taattacttg gattgggata tacggctaat aaattattat cagctgtttt
      661 tgtttcgcga caaccatccg ttaatatttt ggctttgctt aaattaatac atgttgggct
      721 ttcggatata cgtactttgt aaacaccacc cactttggcg taatatgacg aacctgtaac
      781 cgtaggtaat aaaatgttat tgcgatacca ttcatacgtt aatccagtac ctaccggtga
      841 tgcattaagc aacacactat cgcccgggca tactgcactg cttcccacat ttttaagttt
      901 ggcaactaag ggtgttgaca atactattgc cgcactttcg tttgtacatc caaatttatt
      961 ggttgctctt accgtaaaac taccggcatt aattgcatta aaggctgtgt cggtggcgcc
     1021 atttataatg ttgccatttt tataccattg ataggtgtaa gtgctgttat aagtagttga
     1081 taaattaaca ttacctacgc aaaatgaggt agctccattg gctgtaattt taggattggg
     1141 ttgcgctaca acttttacta cattattaat tgtaacggta tcgctgcctt cgggcgaggt
     1201 ggcaattaag gttgcgctat aattgcccgt actattataa gttactacag gattttttag
     1261 cgttgaagtt gatggcgtac cacccggaaa actccaattc cattgattgg ggaaacgttg
     1321 gctcttatca aaatactgta ccgatgattt aatacaaatg gtatcatctg caatttcaaa
     1381 ttgtgggtcg gggatgcatg attg
//
LOCUS       DAOMAA010000694         1402 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_112477_length_1402_cov_3.567929, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000694 DAOMAA010000000
VERSION     DAOMAA010000694.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112477_length_1402_cov_3.567929"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="PLO59_08940"
     CDS             complement(<1..331)
                     /locus_tag="PLO59_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01272.1"
                     /translation="MSKLFQFNHVQKRIFTTLALLAFLGSIFYFFYLPKSEKQVNERL
                     FRNLERIHNNVKVKLDNNYRLLETQLDAYTNPKNSKASINKYIAEQQNQNFELNEIKG
                     DTLGKDIT"
     gene            complement(471..>1402)
                     /locus_tag="PLO59_08945"
     CDS             complement(471..>1402)
                     /locus_tag="PLO59_08945"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HQV01273.1"
                     /translation="ADFVFYLPLDGPQNASTFIDLVKPHAAFFVKYEFWHFYISNLRN
                     RKINCYYFSCVFRPTQTFFQFYGRFHKNMLQRITHIFVQNQASLALLYKHSIPHVTVS
                     GDTRFDRVYANSLQRKSLPIVESFSASQQVFIAGSTWPVDVKLLAAIQNMPVKLIIAP
                     HEITEAHLLQIENAFTKKVVRYSKWQNTDTDAQVLIIDNMGMLSSLYAYGYVAYIGGG
                     FGAGIHNTLEAAVYGCPIIIGPKYQKFVEAKELVQNKGAFVVTNETDLKNILQQLIAN
                     TILHQSIVQKNKNYIVQKKGATEIIMHYLQMNW"
BASE COUNT          420 a          253 c          255 g          474 t
ORIGIN      
        1 tagtaatatc cttacctaag gtatcgcctt ttatttcatt taattcaaaa ttctgatttt
       61 gttgttcggc aatatatttg ttaatggatg ctttgctgtt ttttgggttg gtgtatgcgt
      121 ccagttgtgt ttcgagtaaa cggtagttgt tatccagctt aacttttaca ttattatgta
      181 tgcgctctaa gtttcgaaac agccgttcgt ttacttgctt ttcgctttta ggtagataaa
      241 aaaagtaaaa gatgctgcct aaaaaagcaa gtaaagctaa cgttgtaaaa atgcgttttt
      301 gaacatgatt aaattgaaat agcttcgaca taattacggt tatttaaagc cagcctgtgt
      361 aataacttgt caggcgaatt gtgtggtgtt tacctcaaat gtaatggatg ataaacctaa
      421 tgtcaagggt gtttaccctt gatttcccca atataaaatc ttatcaataa ctaccaattc
      481 atttgcagat aatgcataat aatttcagta gcgccctttt tttgcacgat gtaatttttg
      541 ttcttctgca caatactttg atgcaaaatt gtgtttgcaa taagttgttg taaaatgttt
      601 tttaaatcgg tttcgttggt aacaacaaaa gcacctttgt tttgtaccaa ctctttggct
      661 tctacaaact tttgatattt aggcccaata ataatagggc atccatacac agcagcttcc
      721 aatgtattgt gtatgcctgc accaaatcca ccaccaatat aagccacata tccataagca
      781 tataatgacg aaagcatacc catgttatca attatcagca cctgtgcatc ggtatcagtg
      841 ttttgccatt ttgagtaccg tacaactttt ttcgtaaaag cattttcaat ttgcagcaag
      901 tgtgcttcgg ttatttcatg tggtgcaata attaatttta caggcatgtt ttgtatggct
      961 gccagcaact taacatcgac aggccaggta ctgcctgcaa taaatacttg ttggcttgca
     1021 ctaaaacttt cgactatggg taggcttttc cgttgcaggc tgttggcata aaccctatcg
     1081 aaacgggtat cgccacttac cgttacatgt ggtatgctgt gcttgtataa caatgcaagc
     1141 gatgcctgat tttgtacaaa aatatgcgtt atgcgttgca gcatattttt atgaaacctg
     1201 ccataaaact gaaaaaatgt ttgtgtaggt ctaaacacgc aactaaaata ataacagtta
     1261 atttttcggt tgcgcaagtt gcttatatag aaatgccaaa actcatactt gacaaaaaaa
     1321 gccgcatgtg gctttaccaa atcaataaaa gtgcttgcat tttgagggcc atcaagcggc
     1381 aggtaaaaaa caaaatcggc aa
//
LOCUS       DAOMAA010000695         1399 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_113006_length_1399_cov_2.292411, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000695 DAOMAA010000000
VERSION     DAOMAA010000695.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113006_length_1399_cov_2.292411"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            616..>1172
                     /gene="prs"
                     /locus_tag="PLO59_08950"
     CDS             616..>1172
                     /gene="prs"
                     /locus_tag="PLO59_08950"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015331940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HQV01274.1"
                     /translation="MADNYRQVKIFSGSGSLYMAKHIADAYGQPLGDVTVQRFSDGEI
                     SPFFNESVRGCDVFIIQSTFAPTENLMELLMLIDAAKRASAHYITAVIPYFGFARSDR
                     KDKPRVSIAAKLVANLLTAAGVTRVMTMDLHAPQIQGFFDVPVDHLDATAIFVPYIKQ
                     LNLPNLLIAAPDMGGVHRARSYAKF"
     assembly_gap    1173..1182
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1183..>1399
                     /locus_tag="PLO59_08955"
     CDS             <1183..>1399
                     /locus_tag="PLO59_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008199372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HQV01275.1"
                     /translation="EMAICDKHRKRANEIESMQVIGDVTGKDVILVDDIIDTAGTMTK
                     AAEILLERGATSVRAFCTHPVLSGKAI"
BASE COUNT          433 a          264 c          273 g          419 t
ORIGIN      
        1 atacagtact tgaagaaaaa cccgttgata aatttgtgaa tacaatggta tcgccaggat
       61 taatatttcc ggaagtaaaa gaaaaattaa tggttggata ttgcgcatac accatataat
      121 ttgtgtgcag caacataacc caaataaaca caaatgtttt acaagtcctt ttattaagcg
      181 atgtaaggat agaagtaata aaatttgatt tcattttttt gaagggttaa gatttaaaat
      241 cagtaagcat ttgcctaaat aactttaatt tatttgaaaa aacaaaacct aaattatata
      301 gttttttaag gttgtaatac ctcattttat aattgtataa aaaacgctac aaacttgttg
      361 tttactaaag ttggatgcta tgcatcaaac gctgaaacga tattaaacac caatgaagca
      421 cttcctgttt tttaaagcag ttttttacac ttcgcaattg ggtacgtttt caataacaaa
      481 aaccgaagcc atttttacac gcaaattggt ttacatacaa ttttgcaagc taaatcaggt
      541 gcggttttaa gacggtttta gatgttgcac aaagccaaaa tattagtttc tttgccgacc
      601 ttttttaaaa cccctatggc agacaattac aggcaggtaa aaatattttc gggcagtggc
      661 tccttataca tggccaaaca cattgccgat gcttatgggc agccattggg cgatgtaacc
      721 gtgcagcgtt tcagcgatgg cgaaatatcg ccattcttta acgaaagtgt gcgcggctgc
      781 gatgtgttta ttattcaaag cacttttgca cctaccgaaa atttaatgga actgttaatg
      841 cttattgatg ctgccaaacg tgcatcggca cattacatta cggcagttat accttacttc
      901 ggttttgccc gcagcgaccg taaagacaag ccacgggtaa gtattgccgc caaattagtt
      961 gcaaacttgc ttaccgctgc cggtgtaacc cgtgtaatga ctatggattt acacgctccg
     1021 caaattcagg gtttttttga tgtgccagtt gatcacctgg acgccacagc catttttgta
     1081 ccatacatca aacaattaaa tctgcctaat ttattaattg ccgcacccga tatgggcggt
     1141 gtgcatcgtg cccgttcgta tgccaaattt ttnnnnnnnn nnccgaaatg gctatttgcg
     1201 ataaacaccg caaacgtgcc aatgaaattg aaagcatgca ggtaattggc gatgtaaccg
     1261 gtaaggatgt aattttagtg gatgatatta ttgacactgc cggaaccatg actaaagcag
     1321 ccgaaatttt gttagagaga ggagccacca gtgtacgggc attttgtacg catccggttt
     1381 tatcgggcaa agccatcga
//
LOCUS       DAOMAA010000696         1399 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_113010_length_1399_cov_2.270833, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000696 DAOMAA010000000
VERSION     DAOMAA010000696.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113010_length_1399_cov_2.270833"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1399
                     /locus_tag="PLO59_08960"
     CDS             <1..>1399
                     /locus_tag="PLO59_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01276.1"
                     /translation="GDANQTDGFSANYFGRGNAPAACGNLIGSNTFAGNTTNQNTVVA
                     ATSLGLVTNTNTGATFCSIQSAIDATGTLNGHTLVVAAGNYPQNLTVTKSLTILGPNA
                     GINACSGTRVAEAVLYPSTSAISSGEMIHISAANVTIDGFTLDGDNPVITSGFTSTNG
                     ADIDAAEGITVYEDNKDNLTVQNNIIKNLSYFGVTIFGDYNINAYPPSTGHLITNNSF
                     MDFGTYDATSTIDYWGGGVLLYNSQYAAVTNNCMTNVRIGVQTGNFHQPNPGAATYQN
                     ISGNTMQVRRLGVFHNLQYGTASPYTLANNTITGLANVNEIRVNGILLGSLSVNSTST
                     DNNINLAGISVLSMGYQIWNVAAATPASITSGTISNVDYGVFANNYDGYTSNAPDGAH
                     ANATRLTINPKTTGYGAYVKDNALSTHAPVSLNLTNSFINGGAEGVYFENLSVSGPIN
                     NNHITGNSNKSINATA"
BASE COUNT          429 a          302 c          271 g          397 t
ORIGIN      
        1 taggcgatgc caaccaaact gatggttttt cggctaatta ttttggacgt ggtaatgcac
       61 ctgctgcctg cggtaattta attggaagca acacttttgc aggtaatacc accaatcaaa
      121 acaccgttgt agctgccaca tcgttgggtt tggttaccaa caccaatact ggtgccacgt
      181 tttgctctat ccaatcggca attgatgcta caggtacctt aaatggacat accttagtag
      241 ttgcagcagg taactatccg caaaatctaa cggttacaaa atcgctaacc attttaggcc
      301 ccaatgcagg tataaatgca tgttcgggta cgcgtgttgc cgaagcggtt ttatacccgt
      361 caacttctgc aattagttct ggcgagatga ttcatatatc agcagcaaat gtaaccattg
      421 atggttttac tttagatggc gataatccgg ttattacctc gggctttacc agtaccaacg
      481 gtgccgatat tgatgcagcc gaaggtatta ccgtgtacga agacaataag gacaacctaa
      541 ctgttcaaaa taatattatt aaaaacctct cttattttgg ggtaacaatt tttggtgatt
      601 ataacataaa cgcttaccca ccatcaacag gtcatttaat aaccaataac agttttatgg
      661 attttggtac ttacgatgcc acatctacca ttgattattg gggtggtggt gtgcttttat
      721 acaacagcca gtatgccgct gttaccaata actgtatgac caatgtgcgc ataggtgtac
      781 aaaccggtaa cttccatcaa cctaacccag gcgctgctac ctatcaaaat attagtggca
      841 acaccatgca agtacgcaga ttaggcgtgt ttcataactt gcaatatgga acagcttcgc
      901 cctatacatt ggcaaacaac accattacag gcttagccaa tgtaaacgaa attcgtgtta
      961 atggtatatt attaggatca ctttcagtaa actcaaccag taccgataac aatatcaact
     1021 tagcaggtat cagtgtatta agcatgggct atcagatatg gaatgttgct gcagcaacac
     1081 ctgcaagtat taccagcggt actattagta atgtagatta tggagttttt gctaataatt
     1141 atgatggcta tacttctaat gctcctgatg gcgcacatgc aaatgctacc cgcctaacca
     1201 ttaacccaaa aacaacaggc tatggcgctt atgttaaaga caatgcatta agcacgcatg
     1261 cacctgtaag tttaaacctt acaaacagtt ttattaatgg cggtgccgaa ggtgtgtatt
     1321 tcgaaaacct ttctgttagc ggccctataa acaataacca cataacgggc aacagcaata
     1381 aatctatcaa tgcaacagc
//
LOCUS       DAOMAA010000697         1397 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_113278_length_1397_cov_3.066319, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000697 DAOMAA010000000
VERSION     DAOMAA010000697.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113278_length_1397_cov_3.066319"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="PLO59_08965"
     CDS             <1..282
                     /locus_tag="PLO59_08965"
                     /inference="COORDINATES: protein motif:HMM:NF012305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01277.1"
                     /translation="TENKMNNIFIIKILLFIKNIERSINEVTLESLFAPYGEIVSTKI
                     VYDRITWQSRGFGFIEFEKRADAQKAIEALQGRELKGKAIEISEAIEKR"
     gene            309..758
                     /gene="coaD"
                     /locus_tag="PLO59_08970"
     CDS             309..758
                     /gene="coaD"
                     /locus_tag="PLO59_08970"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008244122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HQV01278.1"
                     /translation="MKIAVFPGSFDPITMGHVDVVTRCLPLFDKIIIGIGTNASKQYL
                     FSQQQREDFIKESFKGVAKIACMHYEKLTVEFCKKQNAQFIIRGLRNSTDFDFETSIA
                     QMNRALSPQIETLFIPCSPQLIAISSTIVRDIIRNGGNAQPFLNFKL"
     gene            complement(748..>1397)
                     /locus_tag="PLO59_08975"
     CDS             complement(748..>1397)
                     /locus_tag="PLO59_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HQV01279.1"
                     /translation="YVIAHGLRKVFTKAYVHVGHDLDACAYATCGDKPGIACILGTGS
                     NSCYFDGNKVIEFNHGLGYIIGDEGSGTYFGKKLLAYYLYKILPAEIQQDFEHRYELT
                     KEIIMENIYYKSGVNVYLASFSKFLSEHNQHPYIKNLVYKGFSEFMTVNVCNIPGYKE
                     VPVHFIGSLAFHFKDILKDAADAHSIQLGSVIQKPIDNLHRYWLDKERKGLLAKV"
BASE COUNT          476 a          256 c          234 g          431 t
ORIGIN      
        1 acagaaaata aaatgaataa tatatttata ataaaaatat tattatttat aaaaaatatt
       61 gaacgttcga taaacgaagt tacactggaa agtttgtttg cgccttatgg cgaaattgta
      121 agtactaaaa tagtttatga tagaattacc tggcaatcgc gcggttttgg ctttatcgaa
      181 ttcgaaaaaa gagccgatgc acaaaaagca attgaagcat tacaagggcg cgaattaaaa
      241 ggtaaggcca tcgaaatttc ggaagctatc gaaaaacgat aacttattat tttgctaaat
      301 catacaatat gaaaattgct gttttcccgg gttcatttga ccctattacc atgggccatg
      361 tggatgtggt aaccaggtgc ttgcctttat ttgataaaat aataattggc attggtacca
      421 atgccagcaa acaatacctc tttagccaac aacaacgcga agattttatt aaagaaagtt
      481 ttaaaggagt tgcaaaaatt gcatgtatgc actacgaaaa acttaccgtt gaattttgta
      541 aaaaacaaaa tgcacaattt ataattcgcg gtcttcgtaa cagcaccgat tttgatttcg
      601 aaacatcaat tgcacaaatg aaccgcgcat taagtccaca aatcgaaacc ctgtttatcc
      661 cttgttcgcc tcagcttatt gccatatcaa gtaccatcgt acgcgatatt attcgcaatg
      721 gtggtaatgc gcagccgttt ttaaatttta aactttagca agcaaccctt tacgctcctt
      781 atctaaccaa taacgatgta agttatcaat tggcttttga attacactac ccaattgaat
      841 gctgtgtgca tcggctgcat cttttaaaat atctttaaag tgaaaagcta acgaaccaat
      901 aaaatgaacg ggcacttctt tataaccggg tatattacat acgttaactg tcataaattc
      961 gctaaagcct ttatacacta aatttttaat atatggatgt tggttatgct cggataaaaa
     1021 tttagaaaat gaagctaaat atacattcac acctgactta tagtagatgt tttccataat
     1081 tatttctttg gttaattcat acctgtgttc aaaatcctgt tgtatttcgg caggtaatat
     1141 tttatataga taataagcca ggagtttttt accaaagtag gtaccactac cttcatctcc
     1201 aataatgtac cctaagccgt ggttaaattc aataacctta ttaccatcaa aatagcagga
     1261 gttagagccg gtgcccaata tgcaagcaat gcccggttta tcgccacagg ttgcatacgc
     1321 gcaagcatct aaatcgtgcc caacatgaac gtatgctttg gtaaaaactt ttcgtagtcc
     1381 atgtgcgata acataat
//
LOCUS       DAOMAA010000698         1396 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_113468_length_1396_cov_2.409396, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000698 DAOMAA010000000
VERSION     DAOMAA010000698.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1396
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113468_length_1396_cov_2.409396"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="PLO59_08980"
     CDS             <1..835
                     /locus_tag="PLO59_08980"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV01280.1"
                     /translation="AAPVIGLLQQYGVSTFAGALDDARATDVTTRVNFSTGMREYFIS
                     GGHTINFMPKQGFILKLDQTAGIMATMSYGNTQDDELNAIEAVGNGGFAGNGLTAYGT
                     FMPVPAPPGPKCYWVKSYYNLVSGCHENPVLSFVMPITLNTQTFIPTFITNTSVMVLP
                     HAQTNDLQNTQCWAVNLPGGSNLKAGVLQDAISLNLQVYPNPTTNDQNINISLVALKD
                     EMVTVTVYDTKGALQYTANEVVSAGTFTMQLPTQKLNSGLYLIRLEGNSIHAQKSFII
                     K"
     gene            complement(899..>1396)
                     /locus_tag="PLO59_08985"
     CDS             complement(899..>1396)
                     /locus_tag="PLO59_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012930311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase (quinone)"
                     /protein_id="HQV01281.1"
                     /translation="VSSPNTPGLRALQDKEPLMQLLMQLQSCNRQKPNPKPILLKIAP
                     DLTNEQLDEIVEIILQTKLTGVVATNTTINRDVEHTDNTTIQQIGAGGLSGKPLKKRA
                     TDVISYIAKKSNKAFVIIGVGGIETATDAQEKIDAGADLVQVYTGFIYQGPAMVKRIL
                     KNMNN"
BASE COUNT          421 a          268 c          270 g          437 t
ORIGIN      
        1 tgcagcacca gttatcggtt tgttgcaaca gtacggagtt tcaacatttg ccggagcact
       61 tgacgatgca agagctactg atgtaaccac acgggttaat tttagcacag gtatgcgcga
      121 gtactttatt tccggaggcc acactatcaa ctttatgccc aaacaaggtt ttattttaaa
      181 gttagatcaa actgctggta taatggctac catgagttat ggaaacactc aagatgatga
      241 gcttaatgca attgaagctg ttggcaacgg tggttttgct ggcaatggac ttactgcata
      301 cggtactttt atgcctgtgc ctgcacctcc aggacctaag tgttactggg ttaaatcata
      361 ttacaattta gtatcgggct gccacgaaaa tcctgtacta agttttgtaa tgccaataac
      421 tttgaataca caaacattta taccaacatt tattaccaat acaagtgtaa tggtattacc
      481 gcatgcacaa accaacgatt tacaaaatac acaatgctgg gctgtaaacc tacctggagg
      541 cagcaaccta aaagcaggcg ttttacaaga tgccatcagc ttgaatttgc aagtgtatcc
      601 aaatccaacc accaacgatc agaatattaa tattagttta gttgcattga aagatgaaat
      661 ggtaactgtt actgtatatg ataccaaagg tgctttgcag tacacagcta atgaagttgt
      721 aagtgccggt acatttacaa tgcaattgcc tacgcaaaaa ctcaactcgg gtttatattt
      781 aataaggctg gaaggcaata gcatacatgc acaaaaatca tttattataa aataatttca
      841 acggttttaa aatttacacc aaacgaccca aaggcagtgc ttttgggtcg tttggttttt
      901 agttattcat atttttcaaa atacgtttta ccatggcagg gccttgataa ataaagccgg
      961 tatatacctg aaccaaatcg gcaccggcat caattttttc ctgtgcatcg gtagcagtct
     1021 caatgccgcc aacaccaata attacaaatg ctttgttaga tttttttgct atatagctta
     1081 taacatcggt agcgcgtttt tttaaaggtt taccacttaa acctccggca ccaatttgtt
     1141 gaattgtagt gttatcggta tgttctacat ctctattaat agtagtgttg gttgcaacaa
     1201 cgcccgtaag tttggtttgc aaaattattt caacaatttc atctaattgt tcattggtta
     1261 aatcgggtgc tatttttagt agtatgggtt tggggtttgg cttttgccgg ttgcaacttt
     1321 gtaattgcat caataactgc attaatggct ccttatcctg caaagcacgt aaacccggag
     1381 tattaggcga tgaaac
//
LOCUS       DAOMAA010000699         1393 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_113958_length_1393_cov_2.458146, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000699 DAOMAA010000000
VERSION     DAOMAA010000699.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113958_length_1393_cov_2.458146"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(204..491)
                     /locus_tag="PLO59_08990"
     CDS             complement(204..491)
                     /locus_tag="PLO59_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020532318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HQV01282.1"
                     /translation="MIRKLNKAFESRVRLGIMSALLVNEEIDFITLKQLLDVSDGNLS
                     SHLTALETLKYIDIEKKFIGRKTNTTYGITRIGRKAFNEHIDALESIIKRK"
     gene            complement(510..1154)
                     /locus_tag="PLO59_08995"
     CDS             complement(510..1154)
                     /locus_tag="PLO59_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01283.1"
                     /translation="MSSEHIQAIHDIRNIMQRSSRFISLSGLSGVFAGVYALCGAAAA
                     YLYTSLEYSQGPYWAAARDSVGNINMGWYTFFFANACTVLLLSVITAFVFTYRKSRKQ
                     GLPIWDYTAKRLIINMAIPLVAGGVFILIIFWHYHLAGLVAPAMLLFYGLALLNAGKY
                     TLVEIRYLGITQIVIGLIATYDIGHGLFYWALGFGVCHIVYGSWMWFKYEKHIK"
BASE COUNT          487 a          283 c          207 g          416 t
ORIGIN      
        1 gcgtaacaac taataaaaca atcagtaaaa cattaaacaa aagcaggtta atacctaact
       61 gctgctggta aaacaatgca gtaaaagcta tggtagccaa acttaaaaca atatcgttct
      121 ttttcattat tcagagtgct ttgtatttca tagtataatt ataaaaaata aggagaacgt
      181 catctcctga tataatattg caactatttt ctttttatta tcgattccaa tgcatcaata
      241 tgttcgttaa aagctttgcg cccaatgcgg gttatgccat aggtggtatt ggttttgcgc
      301 ccaataaact ttttttcaat atcaatgtat ttaagtgttt cgagggcggt aagatggctt
      361 gataaattac cgtcacttac atcaagcaat tgtttaagtg taataaaatc aatttcctcg
      421 ttaaccaata aggcactcat tatacctaac cgcacacggc tttcaaatgc tttatttaat
      481 ttacgaatca tatttatatc aagccaaaat tactttatat gcttttcgta cttaaaccac
      541 atccaggagc catacacaat atgacataca ccaaaaccta aagcccaata aaacaagcca
      601 tggccaatat catacgtagc tattaagcca ataacaattt gtgtaatgcc taaatatctt
      661 atttctacta aagtgtattt acctgcatta agcagtgcca aaccgtaaaa caatagcata
      721 gcgggcgcta ctaaacctgc caaatgataa tgccaaaaaa taataagtat aaaaacacct
      781 ccggcaacta atggaattgc catgttaatt attaaccgct tggccgtata atcccatatt
      841 ggcaaacctt gtttgcgcga cttgcgatac gtaaaaacaa atgcagtaat aaccgatagt
      901 agtaacaccg tacatgcatt ggcaaaaaag aatgtgtacc agcccatgtt aatattacca
      961 accgaatcgc gcgcggcagc ccaatagggc ccctggctgt actcaaggct tgtataaagg
     1021 taagcggcag cagcaccaca taatgcgtaa acacctgcaa acacacccga taaaccactt
     1081 agcgaaataa accggctgct gcgttgcata atattacgta tatcatgtat agcttgtata
     1141 tgctctgagc tcatttttac aatttttata aagtactttg cgttacaaag caaatacaaa
     1201 taaaaattac ctgcaaattt tttaaaaaaa attttaggcc aatctcaaaa tatgatatcg
     1261 caatccctgc tttgtaatac accctactgt tttacaaacc gtaacaccca gtggtttact
     1321 tttataaaat acatacctgt gcttaaatgg tttatatcaa ctgtaaaact gtcattgctt
     1381 atttctttta ttg
//
LOCUS       DAOMAA010000700         1392 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_114087_length_1392_cov_2.627524, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000700 DAOMAA010000000
VERSION     DAOMAA010000700.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114087_length_1392_cov_2.627524"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1392
                     /locus_tag="PLO59_09000"
     CDS             <1..>1392
                     /locus_tag="PLO59_09000"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HQV01284.1"
                     /translation="YKLIEEATFDVAKYLDYPANAFGETYLLHKTEIIEKATPFITKR
                     FLWLENGYSTWPKPETESGKKRKQTYIREIQRGVRDALYADEEFLKSIFVIDETDRII
                     SFHADINVAVDGNLTVVETIKIYNGNGGSNDEVKRGLVRSFPTHYKSKWGFVSTVPFN
                     LLAATCNGLNENYKSKKLDNGISYYFGKADIILERGYYTYVLTYTTSKQIIFRDNNDE
                     LYWNVNGTGWSFTTGMVSCKIVFPLQTKITENNCYTGQQGATTSNCTFKIIEANVITF
                     QTSQPLAANEGLTVSTTIAKGTLIPPATTEKLWSTTTDNAILPLLILAVLFLFCFNYY
                     AWHKVGRDPAAGTIFPQFEPPPDFSPAACGFLIIKSYKSHHFAASLVDYAVNGLVDIE
                     VKPEGLIFKSQVYTFHKPDLITNKSEIINQRYQWYGYYMDKLYNEKAEKGKYNATIAS
                     VYNGLGKHLKDKHE"
BASE COUNT          477 a          268 c          256 g          391 t
ORIGIN      
        1 tacaaactga ttgaagaagc tacttttgat gttgcaaaat atctggacta tccagctaat
       61 gcatttggcg aaacatactt acttcataaa accgaaataa tagaaaaagc aactccgttt
      121 attaccaagc ggtttttatg gcttgaaaac ggatatagca cctggccaaa acctgaaaca
      181 gaatcgggta aaaagcgaaa acaaacttat ataagagaaa tacaacgcgg tgtacgcgat
      241 gcactttatg ccgatgaaga atttcttaaa agcatcttcg ttatagacga aaccgacaga
      301 attatttcct ttcatgccga tattaatgtt gctgtagatg gcaacttaac ggttgttgaa
      361 accattaaga tttacaatgg taatggcggc agcaacgatg aagttaaacg cggcctggtg
      421 cgcagtttcc ctacacatta caaatcaaaa tggggctttg tgtcaacggt ccctttcaat
      481 ttgcttgcgg caacatgcaa cggattaaat gaaaattaca aatccaaaaa acttgataat
      541 ggaatcagct actactttgg taaagctgat attatactcg aaagaggtta ctatacctat
      601 gtgttaacct acaccacatc gaaacaaatt atttttcgtg acaataatga tgaattgtat
      661 tggaatgtga atggaaccgg atggagtttc acaacaggta tggtgagctg taaaattgta
      721 tttccgttgc aaacaaaaat taccgaaaac aattgttaca caggccaaca aggtgctaca
      781 acaagtaact gcaccttcaa aattattgaa gctaatgtta taacatttca aacatcgcaa
      841 ccgcttgcag caaatgaagg actaaccgta tcaactacca ttgcaaaggg caccctaata
      901 ccgccagcta ctactgaaaa attatggagt acaacaacag ataatgcaat tttgccattg
      961 ctaattttgg cagttctgtt cttgttttgc tttaattact atgcctggca taaagtaggc
     1021 cgcgaccctg ctgccggtac catttttcca cagttcgaac ctccgcccga tttttcgcct
     1081 gccgcctgcg gtttcttaat tatcaaaagt tataaatcac atcactttgc tgcatcgtta
     1141 gttgattatg cagttaacgg attggtggat attgaagtaa aacctgaagg cttaattttt
     1201 aaatcacagg tttacacatt tcataagccc gatttaatta ccaataaaag tgaaataatt
     1261 aaccaacgtt atcaatggta tggctattac atggataaac tgtataatga aaaagccgaa
     1321 aaagggaaat acaacgctac cattgcttct gtttataatg gattgggcaa acatctgaaa
     1381 gacaaacatg ag
//
LOCUS       DAOMAA010000701         1390 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_114376_length_1390_cov_3.119101, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000701 DAOMAA010000000
VERSION     DAOMAA010000701.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114376_length_1390_cov_3.119101"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="PLO59_09005"
     CDS             <1..509
                     /locus_tag="PLO59_09005"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HQV01285.1"
                     /translation="GVTTSNACMYADVLIDNDTLRVYNAHLESIRFKKQDYTYVDDIR
                     KNVEQNNYSGILPILKRLKTAFIKRATETEIIKQHKANCKLPLIFCGDFNDTPASYTY
                     KQIAGTLKDAFKDGNAGFGTTYNGVFPAFRIDYILYSPHIAVSQFTTHKIKLSDHCPL
                     TATIHLKP"
     gene            506..1174
                     /locus_tag="PLO59_09010"
     CDS             506..1174
                     /locus_tag="PLO59_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017256778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HQV01286.1"
                     /translation="MKYKIKCAFIFAACLCIQITFAAEPAYKIGLLKYNGGGDWYANL
                     ETSLKNLINFCNKNLNTNISNEQAVVEVGNPEIFNYPFVHMTGHGNVIFNAQEVQNLR
                     NYLMGGGFLHISDSYGIDPYIRREMKKVFPELDFVEVPFNHAIYHQKFNFDNGLPKIH
                     EHDGSNPKGFALVFKGRIICFYDFECDLGDGWENAEVHNDPEEKRQAALKMGANIISY
                     AFSF"
     gene            complement(1190..>1390)
                     /locus_tag="PLO59_09015"
     CDS             complement(1190..>1390)
                     /locus_tag="PLO59_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01287.1"
                     /translation="DLYSSTLYVLTSLAPHLKSGDILIFDEFSVPKHEFLAYTNFIQS
                     YYLQFELIAAANNYFFTAFRMK"
BASE COUNT          489 a          240 c          242 g          419 t
ORIGIN      
        1 taggtgttac taccagtaat gcatgtatgt atgctgatgt tttaattgat aacgatacac
       61 ttcgggttta taatgcacat ttagaaagca tccgttttaa aaaacaagat tacacctacg
      121 ttgatgacat acgtaaaaat gtagagcaaa ataattacag tggcatatta cctattttaa
      181 aacgattaaa aactgcattt ataaaaagag caaccgaaac cgaaataatt aaacaacaca
      241 aggcaaattg caaactgcca cttatttttt gtggcgactt taatgacaca cctgcatcct
      301 atacctataa acaaattgcc ggtactttaa aagatgcctt taaagatggt aacgctggtt
      361 ttggaactac ctataatggc gtatttccgg catttcgaat tgattacata ttatattcgc
      421 cacatatagc agttagtcaa tttacaacgc ataaaataaa attaagcgat cattgcccgc
      481 taacagccac tatacactta aaaccatgaa atataaaatt aaatgtgcct ttatatttgc
      541 tgcatgttta tgtattcaaa taacttttgc tgccgaaccg gcttataaaa ttggcttact
      601 aaaatacaat ggcggaggcg attggtatgc caatttagaa acatcactta aaaacctgat
      661 taacttttgt aacaaaaatt taaataccaa tatcagcaat gagcaagctg ttgttgaagt
      721 gggcaacccc gagattttca actatccatt tgtacatatg acagggcatg gcaatgtgat
      781 ttttaacgca caagaagttc agaatttacg aaactattta atgggcggag gctttttgca
      841 catttccgat agttatggta tcgaccctta tatcagacgc gaaatgaaaa aagtatttcc
      901 ggaattagat tttgttgaag tgccatttaa tcacgccatc tatcatcaga aatttaactt
      961 cgataatggc ctacctaaaa tacatgaaca cgatggctca aaccctaaag ggtttgcatt
     1021 ggtttttaaa ggccgcataa tttgttttta tgatttcgaa tgcgacctgg gcgatggttg
     1081 ggaaaatgcc gaagtacata acgaccctga agaaaaaagg caagctgctt taaaaatggg
     1141 cgccaatatt atttcgtatg catttagttt ttaacccaaa aagtttgtac tacttcattc
     1201 taaacgcagt aaaaaagtaa ttgtttgctg ctgcaatcag ctcaaactgc aggtaatagc
     1261 tttgtataaa attggtgtaa gctaaaaact catgtttggg tactgaaaac tcatcaaaaa
     1321 ttaaaatatc gcccgatttt aaatgtgggg ctaatgaagt aagcacatat aaagtggatg
     1381 aatataaatc
//
LOCUS       DAOMAA010000702         1389 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_114525_length_1389_cov_3.059970, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000702 DAOMAA010000000
VERSION     DAOMAA010000702.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114525_length_1389_cov_3.059970"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="PLO59_09020"
     CDS             complement(<1..966)
                     /locus_tag="PLO59_09020"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HQV01288.1"
                     /translation="MNKQNLYWVFQFTGWFIYTSVNYLSLPLFQTNLQHTGLKLTINF
                     FTGIVVTHLYRNIIKSQQWYKLKFVKLVVQVVLGAALATVVFIVLQVLLETLALYLFQ
                     HTTNLKFNFTKYFPGFGSYQYLNAINSYLVIIVWSVLYFIFHYFENYRSAQLKAASAE
                     AQLTNATLQNLRNQINPHFLFNALNSIKSLAISQPEKSRTATTLLSEILRYSLNSEKN
                     TTVAFETELHIVEEYLQLETIRFGNRLGYKTNIEATTLPLPIPPVMLLTLAENAIKHG
                     ISQRVDGGEIIINSYVNDYYHIVELLNEGQLNTVQKGTGIGVENTL"
     gene            complement(994..1209)
                     /locus_tag="PLO59_09025"
     CDS             complement(994..1209)
                     /locus_tag="PLO59_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01289.1"
                     /translation="MKHLSILSLSVACIFFIGVGIKHGYSHCAKLPVSAIKTSKLETQ
                     KVNLKVEQTTVHFVTEIVKQTINNILH"
BASE COUNT          451 a          218 c          249 g          471 t
ORIGIN      
        1 taaagtgttt tcaacgccaa tgccggtacc tttttgcacg gtatttaatt gaccttcatt
       61 tagcaattca actatatgat agtaatcgtt tacataagaa ttgataatta tttcgccacc
      121 atcaacccgt tgcgatatgc catgttttat tgcgttttcg gccagggtta aaagcataac
      181 cggtggtatc ggtaatggta atgtagttgc ttcaatattt gttttatagc caagcctgtt
      241 tccaaagcgt atggtttcaa gttgcaggta ttcttcaaca atatgtagct cggtttcaaa
      301 agctacggtt gtgttttttt ctgagtttaa agagtagcgt aaaatttctg aaagcagggt
      361 agttgccgta cgcgattttt cgggctgtga aattgccaac gatttgatgc tgtttaatgc
      421 attaaacaga aagtgtgggt tgatttgatt tcttaaattt tgtaaggtgg cattggtaag
      481 ctgcgcttcg gccgatgctg cttttaattg tgcagaacgg tagttttcga aatagtgaaa
      541 aataaaatac aacacactcc acacaataat taccaaataa ctattaatag catttagata
      601 ttgataggat ccaaaacccg ggaaatattt agtgaaattg aactttaaat tagttgtatg
      661 ttgaaacaaa tacaatgcaa gggtttcgag caatacctga agtacaataa acacaacggt
      721 tgctaatgcg gcacctaaaa ctacctgcac tactaatttt acaaacttta atttatacca
      781 ttgttggctt ttaataatat tccgataaag atgggttacc acaataccgg taaaaaaatt
      841 aatagttagt ttcaaaccgg tatgctgaag gttagtttga aaaagtggca gcgataagta
      901 gttaactgat gtataaataa accatcctgt aaactgaaaa acccaatata gattttgttt
      961 attcaatatt acaaaacgat tataaattgc ctattaatgc aatatgttgt ttatggtttg
     1021 ttttacaatt tctgttacaa aatgtactgt agtttgctct accttcaaat taactttttg
     1081 tgtttctaat tttgaagttt ttatggccga aaccggtaac ttggcacaat ggctgtaacc
     1141 gtgctttatg ccaacaccaa taaaaaatat acaggcaaca cttaatgata ggattgatag
     1201 atgtttcatg gtttttatta ttatttcgag caaatatgtt tttaccggtt taataaggaa
     1261 acagaattta caccagtgcc gtttttaaag gataagcgta taatcggtat agaaacaaga
     1321 ataccaacac cgtttgtaac attgatttat tttagcagtc acaaaactaa ttttttgaat
     1381 acaccatca
//
LOCUS       DAOMAA010000703         1387 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_114858_length_1387_cov_3.125375, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000703 DAOMAA010000000
VERSION     DAOMAA010000703.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114858_length_1387_cov_3.125375"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1387
                     /locus_tag="PLO59_09030"
     CDS             <1..>1387
                     /locus_tag="PLO59_09030"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HQV01290.1"
                     /translation="TYIQVIDFYKALDKQYTTAKLLEAGPTDVGLPLHVFVISADAVF
                     NAATLKKQNKTIVLINNGIHAGEPCGIDASMVFAKALLQTSLKQTSPNTVICIIPVYN
                     IDGTLTRGCCSRANQNGPDEYGFRGNYQNLDLNRDFIKADSENAKSFTKIFHQWQPHI
                     FIDTHISNGADYAHNMTLIATQKDKLHPLLSNFMQTIWLPQWYAAMKTNGEPMCPYVD
                     TKDEIPDSGLVGFFEPPRFASGYAALFNTWSFVSESHMLKPYHKQVDATITLLKTMLP
                     LVELHGRQLRFIKHQADSLVKTQDTFPLQYQWQNVTTDSVLFNGYAAVKKISSVSNLP
                     FTTYNQQQPYTKYIPYYNDYKPTLFVTKPKYYLMPQCFKKAVQLLQLNNIKMLPLPCD
                     TAIAVTAYYIDDYKTTERPYEGHYLHSKVKLHTDTQTLIFFKGDYLIPTNQNNNYLVQ
                     VLEPQAADAFFA"
BASE COUNT          451 a          277 c          252 g          407 t
ORIGIN      
        1 tacttatatt caggttattg atttttataa agcattagat aagcaatata caacagctaa
       61 actattggaa gcaggcccaa cggatgtggg attaccgctg cacgtatttg taatttcggc
      121 cgatgctgtt tttaatgcag caacacttaa aaaacaaaac aaaaccatag tgctaattaa
      181 taacggcata catgcaggcg aaccttgtgg tattgatgca agtatggttt ttgcaaaggc
      241 tttgcttcaa acatcattaa aacaaacgtc acccaatacc gttatctgca ttataccggt
      301 ttataatatt gatggcactt taacgcgtgg ctgttgctcg cgcgctaatc aaaacgggcc
      361 cgatgaatat ggctttagag gaaattacca gaacctggac ttgaatcgtg attttattaa
      421 agctgatagt gaaaatgcaa aatcgtttac taaaattttt catcagtggc agccacatat
      481 ttttattgat acacatatca gcaatggtgc cgactatgca cataatatga cgttaatagc
      541 cactcaaaaa gacaaactgc atccgctgct tagcaatttt atgcaaacta tctggctgcc
      601 acaatggtat gctgctatga agactaatgg cgaacccatg tgcccttatg ttgatacgaa
      661 agatgaaata ccggatagtg gtttggttgg attttttgaa ccaccacgtt ttgcatcggg
      721 ctatgcggca ttgtttaata catggtcgtt tgtatcagaa agtcacatgc ttaagcctta
      781 ccataaacaa gttgatgcca ccataacact gcttaaaacc atgctgccat tagtcgaatt
      841 gcatggcaga cagttgcgct ttatcaaaca tcaggcagat agtttggtga aaacacagga
      901 tacgtttcca cttcaatatc aatggcaaaa tgtaactacc gatagtgttt tgtttaatgg
      961 ctatgcagcg gtaaaaaaaa taagttcggt tagtaattta ccttttacaa cttataacca
     1021 acaacaaccc tatacgaagt acatacccta ttacaatgat tacaaaccta cattgtttgt
     1081 taccaaacct aaatattacc tgatgccgca atgctttaaa aaggctgtac aactgctgca
     1141 attaaataat ataaaaatgt tgcctttacc gtgcgatact gctattgcgg ttacagccta
     1201 ttacattgac gattataaaa ctacagagcg accttacgag ggtcattatt tgcacagtaa
     1261 agtaaaactg cataccgata cgcaaacttt aatttttttt aaaggcgatt atttaatccc
     1321 caccaatcaa aacaataact acctggtgca agtgctcgaa ccgcaggctg cggatgcatt
     1381 ttttgca
//
LOCUS       DAOMAA010000704         1386 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_115104_length_1386_cov_1.969947, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000704 DAOMAA010000000
VERSION     DAOMAA010000704.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115104_length_1386_cov_1.969947"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..948)
                     /locus_tag="PLO59_09035"
     CDS             complement(<1..948)
                     /locus_tag="PLO59_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014772403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQV01291.1"
                     /translation="MTLPFITERRLLNFSLAYEAIFHNKLRSLLTALGIIFGVAAVIA
                     MLAIGNGAKQEILEQIKLVGVNNIVIKPASQQKEENVTEETGKKETKKFSPGLNLQDI
                     KAIETTLPNIAKISPEIVIETNFVRNGLARSGKLVGVMPAYFDIIDLQIKQGGNFNKH
                     NMIYGDPVCIIGNAIATKFFNAENPIGQYFKCGNIWYKVIGVMEQKNISGSNIKNLGI
                     RDYNMDVYEPLQTILIRVENRGKLSNEALGGGDFEVIEGESDDEASNTLHQIDRVVVQ
                     VNQSEEMTGTADVLSKMLLRRHNGVTDFEITVPELLLKQQ"
     gene            complement(974..>1386)
                     /locus_tag="PLO59_09040"
     CDS             complement(974..>1386)
                     /locus_tag="PLO59_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="RND transporter"
                     /protein_id="HQV01292.1"
                     /translation="VEVSLDAFPEKKLTGTVTEVANVGEQVAKYDSKVFEVIVQITMK
                     DTTLRPAMTTSNKIVLGKIKNVLSVPLESIHTTDSISYVYVKGSLGINKQQVRTGVSN
                     ENNIVILSGITAGDQVFLNIPASAEKQSVNRLAK"
BASE COUNT          380 a          296 c          227 g          483 t
ORIGIN      
        1 ctgttgttta agcaataact cgggcaccgt aatttcaaaa tcagttactc cgttatgcct
       61 gcgaagcaac atcttactaa gcacatcagc agtgcccgtc atttcttccg attggtttac
      121 ctgtacaaca actctgtcta tctgatgtaa ggtattagat gcctcatcgt ccgactcgcc
      181 ctctattact tcaaaatcgc caccacctaa agcttcattg ctaagtttac ctctgttttc
      241 aacccgtatt aatatggttt gcaagggttc atacacatcc atattgtaat cgcgtatgcc
      301 taagtttttt atgttgctcc ccgaaatatt tttttgctcc atcacaccaa taactttata
      361 ccaaatgtta ccacatttaa aatattgacc aattgggttt tcggcattaa aaaatttagt
      421 ggctatggca tttcctataa tacaaaccgg gtcgccataa atcatattgt gtttattaaa
      481 gttgccgccc tgttttattt gcaaatcaat tatatcgaaa taggcaggca ttacaccaac
      541 caatttgcca ctgcgcgcca atccattacg tacaaagttg gtttctatta caatttcggg
      601 gcttattttg gctatgttgg gcagggtagt ttcaattgct ttaatgtctt gcaaattaag
      661 gccgggagaa aacttctttg tttctttctt accggtttct tctgtaacat tttcttcctt
      721 ttgctggctt gccggtttaa ttacaatgtt atttacacct accaatttta tttgttccaa
      781 tatttcctgc ttggcgccat tgccaatagc caacatagct ataacggctg caacaccaaa
      841 aataattcca agtgctgtaa gcaacgatct gagtttgtta tgaaatatgg cttcgtaagc
      901 caaactaaaa ttaagaagtc tgcgttcggt tataaatggt aatgtcatat tgtttcaata
      961 agtttaaata acattacttg gcaagcctgt ttacactttg tttttcggcc gatgccggga
     1021 tatttaaaaa cacctgatcg cctgcggtaa tacctgatag aattacaata ttattttcat
     1081 tgcttacacc ggtacgcact tgttgtttgt ttatacctaa acttcctttt acatatacat
     1141 aactaatact atcggttgtg tgtattgact ctaaaggcac cgagagcaca ttttttattt
     1201 tacccaaaac aattttatta cttgtagtca tagcaggccg aagggtagta tctttcatgg
     1261 taatctgtac aataacttca aaaactttgc tatcgtactt tgcaacctgc tcgcctacat
     1321 tggcaacttc ggttacggta cccgttagtt ttttttcggg aaatgcatct aaactaactt
     1381 caacgg
//
LOCUS       DAOMAA010000705         1384 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_115425_length_1384_cov_2.255079, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000705 DAOMAA010000000
VERSION     DAOMAA010000705.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115425_length_1384_cov_2.255079"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1107)
                     /locus_tag="PLO59_09045"
     CDS             complement(<1..1107)
                     /locus_tag="PLO59_09045"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006266974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HQV01293.1"
                     /translation="MSQTEMKMPKMGESVAEATIIKWLKNEGDHIEAEEAVLEIATDK
                     VDSEVPAPVAGKLLKRMFNEGDVVQVGSVIALIGDSAADTPVAAPPQTTTVAAPVSAT
                     VETVKHNIQATTAIASTANKFYSPLVKSIATEEKISQAELDAIVGTGAESRVTKHDIL
                     NYVAQKKSGTVSTATQPVKTVADTPKPAVTTTTTTNTSPAVAYTGDHEIVEMDRMRKL
                     IADHMVMSKQTSPHVTSYVEVDVTNLVRWRDKIKGTFEKREKEKITFTPIFIEAIAKA
                     IKDFPMINVSVEGTKIIVKKNINIGMATALPTGNLIVPVIKNADRLNLLGLTKAVNDL
                     ANRARNNKLNPDDIQGGTYTMTNVGTFGNVMGTPI"
BASE COUNT          336 a          262 c          276 g          510 t
ORIGIN      
        1 aatgggtgta cccattacat tgccaaaggt acctacatta gtcatggtat aagtaccacc
       61 ttgaatatcg tcagggttta atttgttgtt gcgtgcacgg ttggctaaat cgtttacggc
      121 tttagttagt cccagtaagt ttagtctatc agcattttta atcacaggta caattaaatt
      181 gcctgttggc aatgcagtgg ccattccaat gttaatattc ttttttacaa taatttttgt
      241 accctcaacg ctaacattaa tcatcggaaa atctttaatg gctttggcta ttgcttcgat
      301 gaaaataggt gtaaatgtta ttttttcttt ttctcgtttt tcaaatgtcc cttttatttt
      361 atcgcgccaa cgcaccagat tggttacatc aacttcaaca taagaggtaa catgtggcga
      421 tgtttgttta ctcataacca tgtggtcggc aatgagtttg cgcatacggt ccatttctac
      481 aatttcatgg tcgccagtgt aggcaacagc agggcttgta ttggtggttg tggttgtggt
      541 tacggctggt tttggggtat cggcaacggt ttttaccggt tgagttgcag ttgaaacagt
      601 acctgatttt ttttgtgcca cgtaatttaa tatatcatgt ttggtaacac gactttcggc
      661 accggtacca acaatagcat ccaattcagc ttgcgaaatt ttttcctcag tagctatact
      721 ttttactaaa ggcgaataaa atttattggc tgttgatgca atggctgttg ttgcttgtat
      781 gttatgtttt actgtttcaa cagttgcgct aacaggggct gcaacagtgg tagtttgagg
      841 tggtgctgca accggtgtat cggcagcact gtcgccaatt aatgcaatta cactacccac
      901 ttgcaccacg tcaccttcgt taaacatgcg cttaagtaat ttacctgcaa ccggtgccgg
      961 tacttcgctg tcaactttat cagttgcaat ttctaaaacg gcttcttctg cttcaatatg
     1021 atcgccttcg ttttttagcc atttaataat tgttgcttcg gcaacactct cgcccatttt
     1081 gggcattttc atttcggttt gcgacatgtg tctgatgctt tttattggca acgaaagtag
     1141 aaaaatatta ttggctgatt atttttttga tgcctacttg agtgctcttt taatactttt
     1201 tttacgcagt cggaatttaa aaatcattcg aataaattaa tcaaccactt cgtttgcgta
     1261 tattttacga accaggtgtt ggttgtttgc taaaattatt tttcgtttta attttaaagt
     1321 tggcgttaat tcgcccgaag ctacagtcca ttcttcgggt acgagttcga aattttttac
     1381 tttt
//
LOCUS       DAOMAA010000706         1383 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_115539_length_1383_cov_3.022590, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000706 DAOMAA010000000
VERSION     DAOMAA010000706.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115539_length_1383_cov_3.022590"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1171)
                     /locus_tag="PLO59_09050"
     CDS             complement(<1..1171)
                     /locus_tag="PLO59_09050"
                     /inference="COORDINATES: protein motif:HMM:NF017464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase N-terminal
                     domain-containing protein"
                     /protein_id="HQV01294.1"
                     /translation="MHYKTIRNICCISVLIATVACNNTVKQTPTFADPLASHTDSSIN
                     PADNFFMFANNGWFKANPIPASERSNGIFQTIQDTINNSIKLICERSSAIADAKLGSN
                     EQKIGDFYFSGMDTVAINKAGTGAIKNQLDLINTLTLENMAQVTASLAKINAAGMFGF
                     YVGVDDKNSALYSVMLMQGGLGLPDRDYYFNTDARTSSIRTAYVNHLKAQFVLLGYDD
                     AKATGAAKDVMQIETDLAKASRTMADRRDPYLNYNKVSADAFYKSNAFNFQTFFTEIG
                     LKNLDSIVVGQPEFFTALNTIIKKYDVEKWKAYAQTQLLNTYASHLSTPFETQAFSFY
                     GTTLSGTKEMKPRWKRMVELTDESLGDLVGQVYVAEYLPKGTKEKLIEIGNNIRSV"
BASE COUNT          401 a          272 c          246 g          464 t
ORIGIN      
        1 acacgcttct gatattgtta cctatttcga tcaatttttc tttagtgcct ttgggcaaat
       61 attcggcaac atatacctgc cctaccaaat cgcccaacga ttcgtccgtt aattcgacca
      121 tacgtttcca acgcggtttc atttctttgg tgcctgataa tgtagtacca taaaacgaaa
      181 aagcctgtgt ttcgaatggc gtacttaaat gactggcata ggtgtttaat agttgcgttt
      241 gtgcgtaggc tttccacttc tcaacatcgt atttttttat aatggtgttt aatgctgtaa
      301 aaaattcggg ctgccctact actatactat ctaaattttt taatccgatt tcagtaaaga
      361 atgtttgaaa gttaaaagcg tttgatttat aaaatgcatc agcacttact ttattataat
      421 ttaaataagg gtcgcgtcta tcggccatgg ttcggctggc ttttgctaaa tcggtttcaa
      481 tttgcattac atctttggct gcaccggttg cttttgcatc atcataaccc aataaaacaa
      541 attgtgcttt caggtgatta acatacgctg tacggataga cgaagtacgt gcatcggtat
      601 taaaataata atcacgatcg ggcaaaccca aacctccctg catcaacata acagaataaa
      661 gcgcactgtt tttatcatcc acacccacat aaaaaccaaa catacctgca gcattaattt
      721 ttgcaaggct tgcagtaact tgcgccatgt tttctaatgt aagtgtgttt attaaatcta
      781 actgattttt aatggcacca gttcctgctt tattaatagc cacagtatcc ataccactaa
      841 aataaaaatc gccaattttt tgctcattac ttccgagctt tgcatcggct atagcactgc
      901 tgcgttcgca tataagttta atactgttgt ttatggtatc ctgtatggtt tgaaaaatac
      961 cattgctacg ctcgctggcc ggtatgggat tggctttaaa ccaaccgttg tttgcaaaca
     1021 taaaaaagtt atcggccggg ttaatactgc tatcggtatg cgatgccaat gggtcggcaa
     1081 aggttggtgt ttgttttaca gtgttgttgc atgcaactgt tgcaatcaga acacttatac
     1141 aacagatgtt gcgaatagtt ttatagtgca tagtatggta tttagtggtg gcgaatgtaa
     1201 attttaaacc acagcttaaa tgttacaata ttgctatttc aaaaatgtgc cgataaaaaa
     1261 aaagctgcac gtatatgcag cttctacttt taaataatat gcgttgaatt attggactac
     1321 caacttaacg gcttttactt ttccgttgcc acgtactaaa atactataca caccttttga
     1381 taa
//
LOCUS       DAOMAA010000707         1383 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_115558_length_1383_cov_2.688253, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000707 DAOMAA010000000
VERSION     DAOMAA010000707.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115558_length_1383_cov_2.688253"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="PLO59_09055"
     CDS             complement(<1..228)
                     /locus_tag="PLO59_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015024602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HQV01295.1"
                     /translation="MKHILCATFCLLVFANLYAQTAKQPPLKQYTIEQLYNNTSVSAA
                     GFNSDESKLLIYSNASGIYNVYELNIADTTTK"
     gene            complement(329..844)
                     /locus_tag="PLO59_09060"
     CDS             complement(329..844)
                     /locus_tag="PLO59_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01296.1"
                     /translation="MKLLTFLFSFCMVCKLNAQSPCTNDTMYHQFDFWIGNWEVYNPK
                     NTKAGDSKISSILDQCVILEEWKSANKQNGVLFEGKSFNTYNASTKQWQQTWVDNVGG
                     CTEYLNGNFSANKMEFKTNAFLIAADTLAIRKLTFYNLGINKVRQHGQISKDNGNTWN
                     TEFDLEYRRKN"
     gene            complement(987..>1383)
                     /locus_tag="PLO59_09065"
     CDS             complement(987..>1383)
                     /locus_tag="PLO59_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009415186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HQV01297.1"
                     /translation="PQHYLCAHNGKEFDFPFLSRRLLINGIALPELLNTAGKKPWEVN
                     HLDSMELWKFGDYKSYTPLNLLAHVFDIASPKDDIDGGMVWQVYWQQNDLARIVTYCQ
                     KDVVTVAQIMLRYNQQPLLKNSEITIADS"
BASE COUNT          394 a          251 c          254 g          484 t
ORIGIN      
        1 tttagttgtg gtatcggcaa tatttaattc gtaaacgtta taaatacccg aagcattact
       61 atatatcaac agcttcgact catcgctgtt aaaacccgca gcgctaacag atgtgttgtt
      121 ataaagttgc tctatggtat attgtttaag tggtggttgc tttgccgttt gtgcatatag
      181 attagcaaaa acgagcagac aaaatgttgc acacaggata tgtttcatag gtgtaggtgt
      241 ttagatggtt gtttaaatat attacatttt aacttagcct ttttcgaaac gttcgtatta
      301 cctaaagcaa attgcccaac tacaaaaatt aattttttcg cctgtattct aaatcaaact
      361 cggtattcca ggtattgcca ttgtcttttg atatttgccc gtgttgcctc actttgttaa
      421 tgcccaaatt ataaaacgtt agtttccgaa tggctaaggt atcggctgca attaaaaagg
      481 catttgtttt aaattccatt ttattggcac tgaaattacc atttaaatat tctgtacaac
      541 cgccaacatt atcaacccag gtttgctgcc attgtttggt gcttgcattg taggtattaa
      601 aacttttgcc ttcaaaaaga acaccgtttt gtttgttggc gcttttccat tcttctaaaa
      661 ttacacattg gtcaagaatg ctactgattt tactatcgcc agctttggtg tttttaggat
      721 tatatacttc ccaattgcca atccaaaaat caaactgatg atacattgta tcgtttgtac
      781 aaggcgactg tgcattaagt ttgcatacca tgcagaacga aaataaaaat gtaagtagtt
      841 tcatatttta attttttggg tttgttttct ttcgcttcgg tttatttaat gttctgacac
      901 aacaattggc agcatgttac aaagcgccat ttccattggg ctttagcaag ttgaagtgaa
      961 tattgtattt gcataaaaag tttaatctaa ctatcggcaa tggtaatttc cgaattttta
     1021 agtaaaggct gttggttgta cctaagcata atttgggcta cggtaaccac atctttttgg
     1081 caataggtaa caatgcgcgc taaatcattt tgttgccaat aaacctgcca taccatacct
     1141 ccgtcaatat catctttagg gctggcaata tcaaacacat gggctaacag gtttagaggc
     1201 gtgtagcttt tataatcgcc aaacttccaa agctccatgc tgtcgaggtg gtttacttcc
     1261 caaggttttt tgccggcagt gtttaacaac tcgggcaatg ctatgccgtt aattagtaaa
     1321 cgtctgctta gaaacggaaa gtcgaactct ttgccattat gtgcacataa ataatgttgt
     1381 ggt
//
LOCUS       DAOMAA010000708         1382 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_115746_length_1382_cov_2.397136, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000708 DAOMAA010000000
VERSION     DAOMAA010000708.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115746_length_1382_cov_2.397136"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="PLO59_09070"
     CDS             <1..704
                     /locus_tag="PLO59_09070"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HQV01298.1"
                     /translation="AAATGTKSIRINNFAGNAIFDDFYTPTFNCTNVTNVQLQYKVAF
                     AVRNTASIDNLKVFVSTDCGKSWTLRQTKSGLNLATVSTAVSSAFVPSATQWRQETVN
                     MNLNASNEPNVRFRFQFTYGTNGLYANNIYIDDINISGLTNINDAFAKDSELSLKPNP
                     TKGIATLTFNVTQAGSYQIQVLDVVGKVCQAQVFNDLSLGINKTEVGNQLKPGLYFVQ
                     VIHNHKMAVLKLVIE"
     gene            789..1328
                     /locus_tag="PLO59_09075"
     CDS             789..1328
                     /locus_tag="PLO59_09075"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HQV01299.1"
                     /translation="MVDKLKYFDVPFAGLSLGNHTYKYTIDQSFFTYFNQQMPQCKFE
                     VSVVLNKQPQILNISFNTVGEMQAICGRCTDEFIMPIVAQNQLLIKFGLQAAEEDVDV
                     MVIPNTETHINLAQYIYEYVMVAIPMHQVHPTDNAGRSQCNQTFLKQIEKRKDESAQL
                     KQQTSDPRWDILKQLNNNN"
BASE COUNT          489 a          254 c          247 g          392 t
ORIGIN      
        1 tagctgccgc aacaggcacc aaatcaatac ggataaataa ctttgccggt aatgcaattt
       61 ttgatgattt ttatacacca acctttaatt gtacaaatgt taccaatgtg caacttcaat
      121 ataaagttgc ctttgctgta agaaataccg ccagtattga taatttgaag gtttttgtga
      181 gtacggattg tggtaagtcc tggacattac ggcaaaccaa gtcggggcta aacttagcaa
      241 ccgtttcaac ggctgtatca tcggcatttg tcccttctgc tacacaatgg cgtcaggaaa
      301 cagttaatat gaacttaaat gcaagcaatg aacccaatgt aagatttagg tttcagttta
      361 cctatggcac caatggccta tatgccaata acatttatat tgacgacata aacatctctg
      421 gcctaacaaa tattaacgat gcttttgcca aagactcaga attaagcctt aagccaaacc
      481 ctacaaaagg tatagctaca ttaacattta atgtaaccca agcaggttcc tatcaaattc
      541 aagttttgga tgtggttgga aaagtttgtc aagctcaggt gtttaacgac ttatctttag
      601 gaataaataa aaccgaagtt ggcaatcagc ttaagccggg attgtatttc gttcaagtaa
      661 tacataacca taaaatggct gtattaaagc tggtaattga atagtgtgat taatataaag
      721 cattcactat tgctaagtta agcctatatt actacatttg ccgtcctttt ttaaaaaggg
      781 aaattgaagt ggttgataaa ttaaaatatt ttgacgtacc gtttgccggt ttatcacttg
      841 gaaaccacac gtataaatac accattgacc aatcgttctt tacatatttt aatcagcaaa
      901 tgcctcaatg taagtttgag gtaagcgttg ttttaaacaa gcaaccgcaa atattaaata
      961 tttcgtttaa tacagtaggc gaaatgcagg caatttgtgg acgctgtacc gatgaattta
     1021 taatgcccat cgtagcccaa aatcagttat tgattaaatt tggtttgcag gcagccgaag
     1081 aggatgtaga tgtaatggta ataccaaaca ccgaaacaca tataaatttg gcacaataca
     1141 tttatgaata tgtaatggta gccataccca tgcatcaggt acatcctaca gataatgcag
     1201 gcagaagcca atgcaaccag acgtttttaa aacaaataga aaaaaggaaa gacgaaagcg
     1261 cccaactaaa acaacaaacc agcgatccgc gttgggatat tttaaaacaa ttaaacaata
     1321 acaattaaca ggtttgtaga agcgaaccaa ataaaattag taccatgcca aatccaaagc
     1381 ac
//
LOCUS       DAOMAA010000709         1380 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_116071_length_1380_cov_2.483774, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000709 DAOMAA010000000
VERSION     DAOMAA010000709.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116071_length_1380_cov_2.483774"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1380)
                     /locus_tag="PLO59_09080"
     CDS             complement(<1..>1380)
                     /locus_tag="PLO59_09080"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HQV01300.1"
                     /translation="VISFIAYSAYRSDTIHITDKKPVVDLGNINFYTQSKTLKQANVV
                     EEAPLIETQIDKIVYNASKDLTVKGGNAGDVLRNVPMVTVDLNGNVELRGTQNVRVLI
                     NNKPSNIMAASVADAMKMIPADEVEKVEVITSPSAKYDAEGTGGIINIITKRKNMQGL
                     SGSVNAGLGTRSSNLFGNLNYRQGRFGIGTSVGGFGFFGNGNLNALRTDYPNGAYSYL
                     KQAGDNSVYGMGPFVQINSDYDINSKNTINASFRLNGFFNRTKGFTDNSLSFDNALYN
                     PLFTNTSDVHTNRLGYDANIDYKKTFLKPNQEFTLSAQLTNNKTNTDYETERDTLENI
                     YYREESLNKSYNKEATFQADYVHPITKKTTIEAGAKSIIRNVESDYHYDIYNFNTQQF
                     EADSLRNNIFVYDQDIYAAYLQGSTSVGKFGFKAGIRYEATEIEGKFLRTTEEPPFAS
                     NYDNWVPSA"
BASE COUNT          367 a          269 c          273 g          471 t
ORIGIN      
        1 ttgccgatgg tacccagtta tcatagttac ttgcaaaggg tggctcttct gttgtacgta
       61 aaaatttacc ttcaatctct gtggcttcat acctaatacc ggctttaaag ccaaacttac
      121 ctaccgatgt cgagccttgt aaataggctg catatatatc ctgatcatat acaaatatgt
      181 tattgcgtaa actgtcggct tcgaactgtt gtgtgttgaa attgtaaatg tcataatgat
      241 agtcgctttc tacattgcgt atgatgcttt ttgcaccggc ctctatggtt gtttttttag
      301 ttattggatg cacataatcg gcctgaaaag tggcttcttt attgtagctt ttatttaagc
      361 tctcttcgcg ataataaata ttttcaaggg tgtcgcgctc ggtttcataa tccgtattgg
      421 ttttgttatt ggttagctgt gccgataagg taaactcctg atttggtttt aaaaaagttt
      481 ttttatagtc aatgttggca tcataaccca aacggttggt atgtacatct gatgtgtttg
      541 taaacaacgg attgtataaa gcattgtcga atgaaaggga gttgtcggta aatcctttgg
      601 tgcggttaaa aaatccattt aatctgaaag aagcatttat ggtgtttttg ctgttaatgt
      661 catagtcgga attaatttgt acgaagggcc ccatgccata tacactgtta tcgcctgctt
      721 gtttcaaata gctgtacgct ccgtttgggt aatcggtacg caaagcattt aaattaccat
      781 tgccaaaaaa accaaagcca cccacactgg taccaatacc aaagcggccc tgcctgtaat
      841 ttaagttgcc aaataagttg ctgctgcggg tacccaagcc tgcattaacc gaaccgctaa
      901 gcccttgcat gtttttacgt ttggttataa tattgataat acctccggtg ccttcggcat
      961 cgtatttagc cgaagggctt gtaataactt ctaccttttc aacttcatcg gcaggtatca
     1021 ttttcatggc atcggcaacg gatgcagcca taatgtttga tggtttgtta ttaataagca
     1081 cacgtacatt ttgtgtacca cgtaactcca cattgccatt taaatcaact gtaaccatgg
     1141 gtacattgcg caatacatca ccggcattac ctcctttaac tgtaaggtct ttacttgcat
     1201 tgtaaactat tttatcaatt tgcgtttcaa taagtggcgc ttcttcaact acattggcct
     1261 gcttaagtgt ttttgattgc gtatagaaat taatattacc taaatcaaca acgggttttt
     1321 tatcggtgat atggatggta tcgctgcggt aggcgctgta tgcaataaaa ctgataacca
//
LOCUS       DAOMAA010000710         1378 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_116357_length_1378_cov_3.232804, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000710 DAOMAA010000000
VERSION     DAOMAA010000710.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116357_length_1378_cov_3.232804"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="PLO59_09085"
     CDS             complement(<1..625)
                     /locus_tag="PLO59_09085"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HQV01301.1"
                     /translation="MNKTFLQVIKYGTMFAIGLLLLWLTFKDVDIEQTWQKIKNANWF
                     WVWLSVICSVAAYFSRAYRWNMLIETAGYKPRFMASVYAVCIAYFANLALPRMGEVTR
                     CGTLGKKENIPFDVLLGTVIVERVIDVLTLLVAMLFLLLWQYDLMYAFLTQHVLKNKP
                     LELDTIQWVLIFTATTVVVFCVFKWIMLPNNAMAKKVNKLVVDYEGEF"
     gene            complement(666..1010)
                     /locus_tag="PLO59_09090"
     CDS             complement(666..1010)
                     /locus_tag="PLO59_09090"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018475476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HQV01302.1"
                     /translation="MNIQVLKSKIHRLAVTQADLHYVGSITLDENLMDAANLIENEKV
                     QVLNVNNGERIETYVIKGERGSGIACLNGPAARKFIVGDIIIVISYASMDFEAAKAWR
                     PAVIFPNADNKL"
     gene            complement(1039..>1378)
                     /locus_tag="PLO59_09095"
     CDS             complement(1039..>1378)
                     /locus_tag="PLO59_09095"
                     /inference="COORDINATES: protein motif:HMM:NF014611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HQV01303.1"
                     /translation="PISIVACATIRETDGLAMSSRNIHLTTEERKQASVIFKTMEACS
                     KLKSDKTPGQLKQWVKNEIERSGIFGLEYVSFVDAATLQPLQQWQLAPQRICLAVTTS
                     KTRLIDNMAL"
BASE COUNT          422 a          285 c          238 g          433 t
ORIGIN      
        1 caaattcacc ttcataatcc actacaagtt tattgacttt ttttgccatt gcattgtttg
       61 gcagcattat ccatttaaaa acacaaaaaa caacaaccgt agtggctgta aaaattaaaa
      121 cccattgtat ggtatctaat tcaagtggct tatttttaag cacatgctgg gttaaaaacg
      181 catacattaa atcgtattgc caaagcagta aaaacaacat ggccactaac agtgttaata
      241 catcaattac acgctcaact attacggtac ccaataatac atcaaatgga atattttctt
      301 ttttacccaa ggtgccacat cgggttactt cgcccatacg gggtaaggct aggttagcaa
      361 aataagctat acatactgca taaaccgaag ccataaaacg gggtttgtat ccggcagttt
      421 caataagcat attccaacga taggctcggc taaagtatgc tgctaccgaa catattacac
      481 tcagccaaac ccaaaaccaa tttgcatttt ttattttttg ccacgtttgt tcgatatcaa
      541 catctttaaa agtaagccat agcaacaaaa ggccaatggc aaacatggta ccgtatttaa
      601 taacttgtaa aaatgtttta ttcaaaactt aatagttggc tttatcattt tgaagcacaa
      661 aaagattata gcttattatc agcattcgga aaaataacag cggggcgcca agcttttgca
      721 gcttcaaaat ccatacttgc gtatgaaatt acaataataa tatcgcccac aataaacttg
      781 cgtgctgccg gcccatttaa acaagcaatc ccgctgccgc gttcgccttt aattacatag
      841 gtttcgatac gctcgccatt attaacgttg agcacttgta ctttttcgtt ctcaattaag
      901 ttggcggcat ccattaaatt ttcgtcaagg gtaatgctgc ctacataatg caaatcagct
      961 tgtgtaacgg caagcctatg gattttcgat tttaaaactt gaatattcat cggtgcaaaa
     1021 gtagtttttt gccaattgct acaaagccat gttatctatt aacctggttt tgctggtagt
     1081 tacagccaaa caaatacgtt ggggcgctaa ctgccattgt tgcagcggtt gtagcgttgc
     1141 cgcatctaca aatgaaacat actctaatcc aaaaatgccg cttctttcaa tttcattttt
     1201 tacccactgt tttaattggc ctggtgtttt atctgatttg agtttgctgc aggcttccat
     1261 tgttttaaat ataactgaag cttgttttct ttcttctgtt gttaagtgga tattgcggct
     1321 actcatggcc aagccatcgg tttcgcgtat ggtggcacaa gcaacaattg aaatggga
//
LOCUS       DAOMAA010000711         1375 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_116910_length_1375_cov_2.743182, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000711 DAOMAA010000000
VERSION     DAOMAA010000711.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116910_length_1375_cov_2.743182"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            501..944
                     /locus_tag="PLO59_09100"
     CDS             501..944
                     /locus_tag="PLO59_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HQV01304.1"
                     /translation="MKQKIILSLLLLFVVIQFFQPSQHNNTTEATQSFADNVPVGNIL
                     KKACNDCHSNYTHYPWYSKVQPAGWYLQNHVNEGRSELNFDSLLSYTAKRQDHKLEEI
                     IEVLELGEMPLSSYTLLHPEAKLDSAEKQILMNWAKEMRIKLNYQ"
     gene            complement(988..1062)
                     /locus_tag="PLO59_09105"
     tRNA            complement(988..1062)
                     /locus_tag="PLO59_09105"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1025..1027),aa:Val,seq:tac)
     gene            complement(1152..>1375)
                     /locus_tag="PLO59_09110"
     CDS             complement(1152..>1375)
                     /locus_tag="PLO59_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HQV01305.1"
                     /translation="LKRLPNQATYYYCAAKLPRALPAKALQELGFANNLIGKTYASVK
                     AALKAAKQAATPNDFIFVGGSTFTVAEAL"
BASE COUNT          455 a          233 c          260 g          427 t
ORIGIN      
        1 gaaaaggcgg agtaagagac caccgttgca tccggtaacg gatgtatgca tgaagtgaat
       61 ttaaatgatg tggctaagtt ataaataggc aattggcatc aaacaatcaa ttcgttttaa
      121 aatggcaatt tgaaacagta attgaattta ctattgagat ttatagttgt gtttgacccc
      181 taagaaacca agtcgctatg tgcttattga attaaattgt attatagaaa tttattgctt
      241 accgcagttg tgaatagatg ataggtaagt aatttttgta ctgcaaacat cccagcaatt
      301 ttttaaaccg gttagcttat tgcaattgag ctttagagct aatgcaagtt gctttgtgtt
      361 tgctcgaaac agttatttaa gcatactccg tttaagatta acgtcaaacg ctaccgggtt
      421 agtgtagtta aaaacaacca tcaattttta tgtaatgcta attatatctt cgaccgatta
      481 aaaaatcttt aagctgtaaa atgaaacaaa aaattatttt aagtctttta ttactgtttg
      541 ttgtaatcca gtttttccag ccaagccaac acaacaatac cacggaggct acgcaatcat
      601 ttgccgacaa tgtgcctgtg ggcaatattt taaaaaaggc ctgcaacgat tgtcatagca
      661 actataccca ttatccctgg tatagtaaag tgcaacctgc aggttggtat ttgcaaaacc
      721 atgtaaacga aggcaggtcg gaacttaatt tcgattcgct gttaagttac actgccaaaa
      781 gacaagacca taaattagaa gaaattatag aagtacttga acttggtgaa atgcctttaa
      841 gtagttacac tttactgcac cccgaagcaa aattagattc agccgaaaaa caaattttaa
      901 tgaattgggc aaaagagatg cgtataaagc ttaattacca atagaaatat acaaaaaaag
      961 cagcataaaa tatgctgctt tctgatgtgg gagctactgg attcgaacca gtgaccctct
     1021 gcttgtaagg cagatgctct gaaccaactg agctaagctc ccataaacaa aggttttttg
     1081 taagcctttt ttgtttgggg ctgcaaatat atacatgcag gtattagttc caaattttaa
     1141 actaaaatat tttaaagtgc ttcggccact gtaaatgtac tgcctccaac aaaaataaaa
     1201 tcgtttggtg tggcagcttg ttttgctgct tttaatgcag cctttacgct tgcataagtt
     1261 ttgccaatta aattatttgc aaaaccgagt tcttgaagtg ctttagccgg caatgccctt
     1321 ggtaatttgg ctgcacaata ataataggta gcctggttag gtaagcgttt taaaa
//
LOCUS       DAOMAA010000712         1374 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_117080_length_1374_cov_2.905989, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000712 DAOMAA010000000
VERSION     DAOMAA010000712.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117080_length_1374_cov_2.905989"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="PLO59_09115"
     CDS             <1..351
                     /locus_tag="PLO59_09115"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002635675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HQV01306.1"
                     /translation="KVGNLLFLSGVGPRQRGSKDIPGVTLDAQGKIIAYDIAIQCHSV
                     FQNIKYILEDAGSCWENIVDVTVFLTNMKDDFATYNALYAQYFKDNLPCRTTLEINCL
                     PTPIAIELKVIATV"
     gene            complement(348..974)
                     /locus_tag="PLO59_09120"
     CDS             complement(348..974)
                     /locus_tag="PLO59_09120"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQV01307.1"
                     /translation="MINRIELQNISKRYYNNWLFKNVNFTFDKQQSVAITGHNGSGKS
                     TLLKIISGFTVPTKGSITYHGQATADTAFEHISMAAPYLDLLHDFTLLENISYFNKFK
                     PLQAGITHGQLIDFAFLNEATHKPYKYFSSGMKQRIKLALAALADTDVVLLDEPLANL
                     DKKGIQWYAEQIVPLLQNRLCIVSTNNPDAEAFFCKHFLEITTYKTEA"
     gene            complement(1020..>1374)
                     /locus_tag="PLO59_09125"
     CDS             complement(1020..>1374)
                     /locus_tag="PLO59_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013455644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein]--UDP-N-
                     acetylglucosamine O-acyltransferase"
                     /protein_id="HQV01308.1"
                     /translation="GGLSAVNQFVHIGSHVMISGGSLVRKDVPPFTKSAHEPLAYVGL
                     NVVGLRRRNFTNEQINNVQEIYRHLFVNGHTTAKAVKLIEEQIAQGEERDLILDFVKS
                     SQRGLMKGYVSDSEA"
BASE COUNT          393 a          263 c          273 g          445 t
ORIGIN      
        1 aaggttggca atttgctttt tctatcgggt gtggggccca ggcagcgtgg cagcaaagat
       61 atacccggtg taactttaga tgcccaagga aaaattatag cgtatgatat agccatacaa
      121 tgtcatagtg tatttcaaaa tataaagtac attttggaag atgctggaag ctgttgggaa
      181 aacattgtgg atgtaaccgt ttttttaacc aatatgaaag atgattttgc aacctacaat
      241 gctttgtatg cacaatattt taaagataat ttaccctgcc gtactaccct tgaaataaat
      301 tgtttgccaa cccctattgc catcgaacta aaggtaattg ccacggttta ggcttcggtt
      361 ttgtaagtag taatttctaa aaaatgttta caaaaaaagg cttcggcatc gggattattg
      421 gtgctaacaa tacatagtct gttttgtaat aaaggcacta tttgttcggc ataccactgt
      481 atgccttttt tatctaagtt agccaaaggc tcgtctaaca aaaccacatc tgtatcggct
      541 aatgcagcca gagccagctt tatgcgttgt ttcataccag acgaaaaata tttgtacggt
      601 ttatgtgtgg cttcgttcag aaatgcaaag tcaattaatt gcccatgcgt tataccggct
      661 tgcaatggct taaatttatt gaagtagctg atattttcga gcagcgtaaa atcgtgtaac
      721 aaatctaaat aaggcgcagc catactaata tgttcaaatg cggtatcggc cgttgcctgc
      781 ccatgatatg tgatgctgcc tttggtaggt acggtaaacc ccgaaataat tttaagcagt
      841 gttgatttgc ccgaaccatt atggcctgta attgcaactg attgctgctt atcgaatgtg
      901 aaatttacat ttttaaacag ccagttattg tagtagcgtt tgcttatgtt ttgtaactct
      961 atacgattta tcattcacac ctttttaaat gctgcagtaa ggtatatgaa gcttttggat
     1021 tatgcttcgc tatcgcttac atagcctttc attaaaccgc gttgtgatga ttttacaaag
     1081 tccaatatca aatcgcgctc ttcgccttgg gcaatttgtt cttcaattaa ttttacagct
     1141 ttggcagttg tgtgtccgtt aacaaacaaa tggcggtaaa tttcctgcac attatttatc
     1201 tgttcgttgg taaagttgcg cctgcgtaag cccacaacat ttaagccaac ataagccaat
     1261 ggctcatgtg cacttttggt aaaaggtggt acatcttttc gtaccaatga gccacccgaa
     1321 atcattacat gcgaaccgat gtgtacaaat tgatttacgg cacttaagcc cccg
//
LOCUS       DAOMAA010000713         1373 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_117217_length_1373_cov_3.110015, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000713 DAOMAA010000000
VERSION     DAOMAA010000713.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117217_length_1373_cov_3.110015"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="PLO59_09130"
     CDS             complement(<1..993)
                     /locus_tag="PLO59_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01309.1"
                     /translation="MAFADSCSLIIDKYFLNSYEPTYTKAIVENQCVFNFKIDSRVTA
                     TFHYNNQTIPLIISPNSVIEIFVGTDSLYNSIRLHENEKHNNQFLLYFNKQFKNDFAD
                     SLIFETMLSNKADVFEMKAFKLQQNQLDFIRSIIDSLKPDDATVSYLKNRVKYLYYYK
                     MQAFAIINANADKGLTVKPMPDLLLENVGPNLLNDELLGIDTYRKFIEYYVIYQTSKL
                     NGFNKFTDFNISMDKKIAFANTRLTPRTKSYYVANYLNNHINEVLPATAKYNYQQLTN
                     FDVFGYAALVETVCKNRINAKVEKTKTSVSDYRDQKIMGVDGKYFTLQDFKGKVV"
     gene            complement(1086..>1373)
                     /locus_tag="PLO59_09135"
     CDS             complement(1086..>1373)
                     /locus_tag="PLO59_09135"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HQV01310.1"
                     /translation="ISKHLNLTVAAFTDKYLERDKKDWVNKQQPCQFLGSDNKCSIYE
                     IRPVDCAGFPHTHKNNPGFVGASSVNRDFFWRCPIVYHVVDSIFDKVVNQK"
BASE COUNT          453 a          238 c          211 g          471 t
ORIGIN      
        1 cacaaccttg cctttaaaat cctgcagtgt aaagtacttg ccatcaactc ccataatttt
       61 ctgatctcga taatcgctaa cggatgtttt cgttttttca actttagcgt tgatgcggtt
      121 tttacaaact gtttcaacta atgcagcata gccaaataca tcaaaattag ttagctgttg
      181 atagttgtat tttgcagtag cgggtaatac ttcattaata tgattattga ggtagtttgc
      241 cacatagtaa ctttttgtgc gtggtgttaa ccttgtgttt gcaaatgcaa ttttcttatc
      301 catcgaaata ttaaaatcgg taaacttatt aaaaccgtta agttttgatg tttgatatat
      361 aacatagtat tcaataaact ttcgatacgt atcaatgcca agcaattcat catttaaaag
      421 attaggccca acattttcta acaacaaatc aggcataggt tttacagtta aacctttgtc
      481 agcgtttgca ttgattatgg caaatgcctg cattttatag tagtaaaggt attttacccg
      541 gttttttaaa taagatacag ttgcatcatc gggttttaac gaatcaataa tgcttctgat
      601 aaaatccagt tgattttgtt gcaacttaaa cgccttcatt tcaaaaacat cagccttgtt
      661 actaagcatg gtttcaaaaa tcaaactatc tgcaaaatca tttttaaact gcttattaaa
      721 gtataataga aactgattgt tatgtttttc gttttcatgc agtctaattg aattgtacaa
      781 actatccgtg ccaacgaata tttctattac gctattgggc gaaattatta atgggattgt
      841 ttgattgttg taatgaaaag tggccgttac acgactgtca attttaaaat taaaaacaca
      901 ttggttttca actattgctt tagtataagt tggttcataa gaatttaaaa agtacttatc
      961 aattatcaaa ctgcaactat cagcaaaagc caatttaaaa ttgcacttaa tttctgcagc
     1021 ctttgctaca ttaacaagta acgataaaaa aagtacaccg aaagttctaa gtaaaatttt
     1081 gtccattatt tctgattaac aactttatca aaaatggaat caaccacatg ataaacaatt
     1141 gggcaacgcc aaaaaaaatc ccggttaacc gatgaagcgc ctacaaaacc cggattgttt
     1201 ttatgcgtat gtggaaagcc ggcacaatca accggcctta tctcataaat cgaacattta
     1261 ttgtcgctac ctaaaaactg acaaggttgt tgtttattaa cccagtcttt tttatcgcgt
     1321 tccaaatact tatcggtaaa ggccgcaacg gttaaattta aatgtttcga aat
//
LOCUS       DAOMAA010000714         1370 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_117827_length_1370_cov_1.946008, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000714 DAOMAA010000000
VERSION     DAOMAA010000714.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117827_length_1370_cov_1.946008"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..705)
                     /locus_tag="PLO59_09140"
     CDS             complement(70..705)
                     /locus_tag="PLO59_09140"
                     /inference="COORDINATES: protein motif:HMM:NF025028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY domain-containing protein"
                     /protein_id="HQV01311.1"
                     /translation="MPYSLLNHFIKTVAFAALLLVISACSSGYKKEAGQWVWVSYDEM
                     EGKRITMIEGVDAQTFNVLTNENYAVDKNAVYYTHRKIKYADPATFKVLTDGYAVDKY
                     HVFFDYGMILFADPATFQSLGFLYSKDKNDVYCGMIPLHLSKADLNLFTVTGSKANFS
                     NTKSAVLTSHFIELNPEFAWIDTLQLERIIVGDYTTGQAGPKKFYGFKQVK"
     gene            complement(792..>1370)
                     /locus_tag="PLO59_09145"
     CDS             complement(792..>1370)
                     /locus_tag="PLO59_09145"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004920747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HQV01312.1"
                     /translation="TWEKTNPPPNFSCRYFTYSTEQIIWARKSEKLPHYFNYELMKQL
                     NGDKQMKDVWKLPAIAPWEKSCGKHPTQKPLSVLSRLILASTKPNAWILDPFAGSSTT
                     GIAANLANRRFLGIDQEEEFLSISKNRKHEIENPKISATYKQRISGFNDNKELELFLA
                     EEPKTEYKTALNLMQRKVEQATKVLQKSEGKV"
BASE COUNT          384 a          251 c          230 g          505 t
ORIGIN      
        1 tatcgttgtg ttttatttcc atggtatttt actattaaaa ccaaataaac attgcaccct
       61 ttaatttgat tatttaactt gtttaaaccc gtaaaacttt ttggggcctg cttggcccgt
      121 tgtatagtcg ccaacaataa ttcgttcaag ttgtaaggta tctatccagg caaactcagg
      181 gtttagttca ataaagtgtg atgttagtac agccgatttg gtgttcgaaa aattggcttt
      241 gctgccggta actgtaaata ggtttaaatc tgctttcgat aagtgcaatg gtatcatacc
      301 gcaatacaca tcgtttttat ctttcgaata taagaagccc aacgattgaa aagttgccgg
      361 gtcggcaaat agtatcatac cataatcaaa aaacacatga tacttatcga cagcatatcc
      421 atcggttaac actttaaaag tagccgggtc ggcatatttt attttgcgat gtgtatagta
      481 aactgcgttt ttatctactg cataattctc gtttgttaaa acattaaatg tttgcgcatc
      541 tacgccttct atcatggtaa tacgcttacc ttccatttcg tcatacgaaa cccaaaccca
      601 ttgtccggct tcttttttat atccactgct acatgcactt ataaccaaaa gcaaagctgc
      661 aaatgctaca gtttttataa aatgatttaa aagagaataa ggcataacat tttgaggttt
      721 tacgaagtgt attaatctta aagctgcttt tcaactttgt aacaaaattt gctaacacat
      781 ccgcaattaa attaaacttt tccctcactt ttttgcaaaa cttttgttgc ttgttcgacc
      841 tttctctgca tcaaattaag agctgtttta tattctgttt ttggttcttc ggcaagaaat
      901 aattcaagtt ctttattgtc attgaaccca ctaattcttt gtttgtatgt cgcagatatt
      961 ttaggatttt caatttcatg ttttctgttt ttgctgattg ataaaaactc ttcttcctgg
     1021 tcaatcccta aaaatcgtct gttagctaag tttgctgcaa ttcctgttgt cgagcttcct
     1081 gcgaaagggt ctaaaatcca agcatttggt tttgttgaag ccaaaataag ccgtgacaaa
     1141 accgataaag gtttttgagt tgggtgtttt ccacacgact tttcccaagg tgcaattgct
     1201 ggaagtttcc aaacgtcttt catttgtttg tcgccattaa gttgtttcat taattcgtag
     1261 ttgaaataat gcggtagctt ttcgcttttt cttgcccaaa ttatttgttc agttgaatat
     1321 gtaaaatatc tacacgaaaa atttggtggt ggatttgttt tttcccaagt
//
LOCUS       DAOMAA010000715         1367 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_118321_length_1367_cov_2.266768, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000715 DAOMAA010000000
VERSION     DAOMAA010000715.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118321_length_1367_cov_2.266768"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1219
                     /locus_tag="PLO59_09150"
     CDS             <1..1219
                     /locus_tag="PLO59_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HQV01313.1"
                     /translation="MDGKKTTDKISTTVGFRELKFEKGNVFLNGENIKLMGIEWTAGS
                     NPDYGFAESDSVIVSQCKLMKNVNAIFTRQHFQQDDLFYACCDRFGMLVQQEVPLWGY
                     ETPANDTISNIAHKQLQSMIRNMYNHVSVFSWGVGNELRARDADMKAMIKGLLQHARN
                     IDSTRFTAYVSNSLTQGFYNSPSFVPDAAADGDYLMMNEYGGSWWDIPTGNIHAYLDS
                     VHATYPKLPFFISEFGLCEPNFKGGDERRIEDLIYHMGIYETKPYISGAIYFDLTDYR
                     THYPGTNDTTKYRRRIHGVYDMYGQPKPSMKILRELSAPIEIQNVRHWQKDKLNLLLF
                     GSMGLPQHAAKGYNLYVSSEAQNYAATKAYPLPDIMPGQRINFEVDEINSKQTYITIV
                     RPNGFITSQKIFD"
BASE COUNT          465 a          248 c          260 g          394 t
ORIGIN      
        1 aatggatgga aaaaaaacta ccgataaaat ttcgactacg gtaggattcc gcgagttaaa
       61 atttgaaaaa ggcaatgttt ttctaaatgg cgaaaatata aaactgatgg gcattgaatg
      121 gactgcaggc agtaatccgg attacggttt tgctgaatca gattcggtaa ttgtcagtca
      181 atgcaaactt atgaaaaatg tgaatgccat ttttacccgc caacactttc aacaagacga
      241 cttgttttat gcatgttgcg atcgctttgg tatgcttgtg cagcaagaag taccattatg
      301 gggttatgaa acgcccgcta acgataccat aagtaacatt gcgcacaaac aacttcaaag
      361 tatgatacgt aacatgtaca atcatgtatc cgttttctct tggggtgtag gtaatgaact
      421 gagagcacgc gatgccgata tgaaggcgat gattaaaggg ctattacagc atgcacgtaa
      481 tatagattcg acaaggttta cagcctatgt tagcaattcg cttactcagg gtttttataa
      541 cagcccatca tttgtgcccg atgcagctgc cgatggcgat tacctgatga tgaatgaata
      601 tggtggcagt tggtgggata taccaacagg taatatacat gcttacttag acagtgtgca
      661 tgcaacgtat cccaagttac ctttttttat ttctgaattt ggtttatgcg aacccaattt
      721 taaaggtggt gacgagcgca gaatagaaga cttaatttac cacatgggca tttacgaaac
      781 caaaccatac atttccggtg ccatatattt tgacttaact gattaccgca cacattatcc
      841 cggcaccaac gatacaacaa agtaccgcag acgtatacat ggtgtttatg atatgtatgg
      901 ccagcccaaa ccatccatga aaatattacg cgaactttca gctcctatag aaattcaaaa
      961 cgtaaggcat tggcaaaaag ataaattaaa tttattgctg tttggcagta tgggtttgcc
     1021 ccaacatgca gcaaaaggtt ataacctata tgtttcatct gaagcacaaa actatgcagc
     1081 aacaaaggca tatccattgc ctgatataat gccgggccaa cgtattaatt ttgaagttga
     1141 tgaaataaac agcaaacaaa cttatataac cattgtaagg cctaatggat ttattacatc
     1201 tcaaaaaata tttgattaga gataattaaa atttgaattt ataacattaa gaaattgata
     1261 ttaatgttat ttcaaattaa ttacttacta ccaaacaaac ggaacattta ttagacaact
     1321 cttaaacaaa acagcgtgga acagttgttg caataactat tgtaatc
//
LOCUS       DAOMAA010000716         1366 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_118453_length_1366_cov_2.531655, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000716 DAOMAA010000000
VERSION     DAOMAA010000716.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118453_length_1366_cov_2.531655"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1089
                     /locus_tag="PLO59_09155"
     CDS             <1..1089
                     /locus_tag="PLO59_09155"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 family protein"
                     /protein_id="HQV01314.1"
                     /translation="IVGAINDPIFGKTTASVYSSMILSSFNFNYGSTATADSIVLCLA
                     LKTFYGDTTTPIHFKVYRLDSVLPKDTVYYSNSTLSASTLIGDAIVTVKPSDSITEYA
                     VKRSAHIRIPLFTNLATELISKSGGPEYVDNTAFANYFKGIYITAEPEAAVDKGCLYQ
                     WDYKSALSRMTVYYKLNTDSDSLAYSYNFDELLRFNNYQHNYTGTEVGNALTFPTVGQ
                     NNLYVRAMAGTKAFIQFPTLKSIIDERAFSVNNAILVFKAEPNTKYTPNANLTLALVD
                     SAGNQASFAPDNNELANAADGTYNSSTNEYKFNITRYVNRILAEKHVDYGIYLRSASS
                     IINFNRVKLNGTGNVTGRLKLELTYSKY"
     gene            1095..>1366
                     /locus_tag="PLO59_09160"
     CDS             1095..>1366
                     /locus_tag="PLO59_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HQV01315.1"
                     /translation="MCGIVAYIGKRQAYPIIVKGLQRLEYRGYDSAGVALLHQGQMNI
                     YKKAGKVTDLIKFAEGKELNGNIGMGHTRWATHGEPNDRNAHPHWS"
BASE COUNT          439 a          298 c          270 g          359 t
ORIGIN      
        1 attgtgggcg ccataaacga ccccatattt ggcaaaacca cagcaagcgt ttactcgtca
       61 atgattttat cgagcttcaa ttttaattat ggcagtacgg ctacagccga ttcgatagta
      121 ctttgtttgg ctttaaaaac tttttatggt gataccacta cacccataca ttttaaggtt
      181 tacaggttag atagtgtatt accaaaggat accgtttact atagcaacag cactttaagc
      241 gcaagtacat taattggcga tgccatagta accgtaaagc ccagcgacag cattaccgaa
      301 tatgccgtta aacgcagtgc ccatatacgc atcccattat ttacaaacct ggctacagag
      361 ttaattagca agtcgggtgg gcccgagtat gttgacaata ctgcctttgc caattacttt
      421 aaaggcatat acattactgc cgaacccgaa gccgcagttg ataaagggtg cttgtatcaa
      481 tgggattata aatcggcatt aagccgcatg actgtttatt acaagctaaa taccgatagc
      541 gattcattag cctacagtta taactttgat gaattactgc gctttaataa ttaccaacat
      601 aactataccg gtacagaagt gggcaatgcc ctaacatttc ccactgtggg gcaaaacaac
      661 ttgtatgtac gagctatggc cggtaccaaa gcattcattc agttcccaac tttaaaaagt
      721 ataattgacg aacgggcttt ttcggtaaat aatgccatat tggtttttaa agccgaaccc
      781 aataccaaat acacccctaa tgccaattta acgttggctt tggttgactc ggccggcaat
      841 caggcatctt ttgcgcccga caacaatgag ttagccaatg cagccgatgg cacgtataac
      901 agcagcacca atgaatataa gtttaacata acacgatacg taaaccgcat acttgccgaa
      961 aaacatgttg actatggcat ttatctgcgc agtgcatcct caataattaa ttttaaccgg
     1021 gttaaactaa acggcaccgg caatgtaacc ggacgtttaa aattagaact tacttactca
     1081 aaatattaaa aactatgtgt ggaattgtag catatattgg taagcgccaa gcctatccca
     1141 ttattgtaaa aggtttacaa aggcttgaat accgtggata cgacagtgca ggtgtagcat
     1201 tgctgcatca ggggcaaatg aatatttaca aaaaagccgg taaggttacc gacttaataa
     1261 aatttgccga aggcaaagaa cttaacggca acataggtat gggccataca cgctgggcta
     1321 cccatggcga accaaacgat agaaacgccc acccgcactg gagcga
//
LOCUS       DAOMAA010000717         1366 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_118490_length_1366_cov_2.173150, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000717 DAOMAA010000000
VERSION     DAOMAA010000717.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118490_length_1366_cov_2.173150"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="PLO59_09165"
     CDS             <1..659
                     /locus_tag="PLO59_09165"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020607154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HQV01316.1"
                     /translation="CLTSIPNIVRIFTEETPYNPQSPYAASKAASDHLVRAYQNTYQL
                     PTVISNCSNNYGPNHFPEKLIPLAINNIKNNLPIPIYGKGENVRDWLYVEDHAAAIDL
                     IFHKGTVGETYNIGGHNEWHNIDVIKLLCQIMDEALNRTAGTSAQLITYVKDRAGHDL
                     RYAIDSGKLQHTLGWQPSLNFEKGLRQTVSWYLNNETWLNNVTSGAYKNYYQMQYGNR
                     "
     gene            680..>1366
                     /locus_tag="PLO59_09170"
     CDS             680..>1366
                     /locus_tag="PLO59_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019946844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQV01317.1"
                     /translation="MYNTSFHTPTLNNKAVLITGGAGFIGSNIVAYLMQHGVRKVRVL
                     DNLSNGFKKNIAPYLSNPSFEFIEGDITNYETCLKVCEGIDVVTHQAALGSVPRSIKD
                     PVATHAANATGFINMLWAAKNCNVNRFIYASSSSVYGDAQQSPKIEANLGKPLSPYAV
                     SKLTNELYASIFAHTYGMQLIGLRYFNVFGPQQDPNGPYAAAIPIFMDALLNNQTAFI
                     NGDGLTTRDFT"
BASE COUNT          436 a          305 c          250 g          375 t
ORIGIN      
        1 attgcttgac ttccattcct aacattgtaa gaattttcac cgaagaaaca ccatacaatc
       61 cgcaaagccc ttatgccgct tcgaaagcag ccagcgacca tttggtgcgt gcctatcaaa
      121 acacctatca gttgccaacg gtaatttcta attgcagcaa taattatggc cccaaccatt
      181 ttcccgaaaa attaattccc ttagccataa acaatattaa aaacaactta ccaataccta
      241 tttacggcaa aggcgaaaac gtgcgcgatt ggttgtatgt tgaagatcat gcagcagcta
      301 ttgacttaat tttccacaaa ggaaccgttg gcgaaaccta taacattggt ggccataatg
      361 aatggcacaa cattgatgta attaaattgc tttgccaaat aatggacgaa gccttaaatc
      421 gcactgccgg cacaagcgct caacttatta cctatgtaaa agatcgtgct gggcacgact
      481 tgcgctatgc tattgattcg ggcaaattgc agcataccct tggctggcaa cccagcctta
      541 atttcgaaaa agggttgcgc caaaccgtta gctggtattt aaataatgaa acatggctta
      601 ataatgtaac ttcgggcgcc tacaaaaact attatcaaat gcaatatggt aaccgataag
      661 gctactttat aacaagctta tgtataacac ctcatttcat acacccacac ttaataataa
      721 ggcagtacta ataaccggtg gtgctggttt tataggatca aacattgttg cctacttaat
      781 gcaacatggt gtgcgtaaag tgcgtgtact tgataaccta tcaaacggat ttaaaaaaaa
      841 cattgcacca tacttatcaa acccctcttt cgaatttata gaaggcgata taaccaatta
      901 tgaaacctgc ttaaaagtat gtgagggtat tgatgtggtt acacatcaag ctgcattggg
      961 ttcggtacca cgtagtatta aagacccggt ggctacacat gcagccaatg caaccggctt
     1021 tataaacatg ttgtgggcag caaaaaattg taatgtaaat cgttttatat atgccagcag
     1081 ttcatcagta tatggcgatg cacaacaatc gccaaaaatc gaagccaact taggcaaacc
     1141 cctatcaccg tatgcagttt caaaactaac caacgaactg tatgcctcta ttttcgcaca
     1201 tacgtatggc atgcaattaa taggcttgcg ttactttaat gtgtttggcc cacagcaaga
     1261 tcccaatggc ccgtatgcag ccgctatccc catttttatg gatgcattgc ttaataacca
     1321 aaccgctttt attaatggcg atggactaac tacccgtgat tttacg
//
LOCUS       DAOMAA010000718         1365 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_118592_length_1365_cov_3.181679, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000718 DAOMAA010000000
VERSION     DAOMAA010000718.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118592_length_1365_cov_3.181679"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="PLO59_09175"
     CDS             <1..197
                     /locus_tag="PLO59_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01318.1"
                     /translation="YDEHHPIINIDRKTKFTHYTNLNKTVHNTRQLLNDFTFDTVSNV
                     VANYKQNHVVEQFTIKVLQG"
     gene            220..1236
                     /locus_tag="PLO59_09180"
     CDS             220..1236
                     /locus_tag="PLO59_09180"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQV01319.1"
                     /translation="MPLVRQVLISGNSLHAHSYDFQKWHWKYFDLKHAKPLIYLCLEG
                     DEIIGYYHCPVYEGIIETKPVLIAMVQDVGITEKARGKGVFAQLAKFANHDLAKNQID
                     FVITYPNDKSLPTFIKYNQYQLIATLDALILPVKSSLIIAQKLPRFLAEIFGASIDTL
                     FSTTHKTWQPQQIGIVPEFNTEMIQIFELHYNQFQNYIKRSMHYLKWRFEAKPNHTHF
                     IINASFDNKIHTVLVFGIEDLLGVKAAVLLDYATTNPNAMANAMLQLAKNSGKYFNHD
                     VAMFYTSSLPHNSKVLQKCGFIKIPKKLNPRPLNLMAKNITTTNDAYLAGKWQVTLSE
                     WDVL"
BASE COUNT          498 a          251 c          213 g          403 t
ORIGIN      
        1 agtatgacga acaccacccc ataataaata ttgatagaaa aaccaagttt actcattata
       61 ccaacctaaa taaaacggtt cacaacaccc ggcaattatt aaacgatttt acctttgata
      121 ccgtaagtaa tgtagttgca aattataaac aaaaccatgt tgtcgaacag tttaccatta
      181 aggttttgca aggctgaacc aagtcattac aatcaattgg tgcctttggt aaggcaagtt
      241 ttaatatccg gtaattcatt acatgcacat agttacgatt ttcaaaagtg gcattggaaa
      301 tattttgatt taaagcatgc taagccatta atatacttgt gtttagaagg cgatgaaata
      361 attggttatt atcattgtcc ggtttatgaa ggaataattg aaacaaaacc ggttttaata
      421 gccatggtgc aggatgttgg cataactgaa aaagcacgcg gcaaaggcgt ttttgcacaa
      481 cttgccaaat ttgcaaacca tgatttggcc aaaaatcaga ttgattttgt aattacctat
      541 cccaatgata agagtttgcc aacctttatc aaatacaacc aatatcaatt gatagctacc
      601 ttagacgcat taatattacc tgtaaaatca tctttaataa ttgctcaaaa acttcctagg
      661 tttttagctg aaatttttgg cgcttcaata gatacactat ttagcaccac acataaaact
      721 tggcaaccac aacaaattgg tattgtacct gaatttaaca cagaaatgat tcagattttt
      781 gagctgcact acaaccaatt tcaaaactat ataaagcgca gcatgcatta tcttaaatgg
      841 cgtttcgaag ctaaacctaa ccatacacac tttattataa atgcaagctt cgacaacaaa
      901 atacataccg tgcttgtatt tggaatcgaa gatttattag gtgtaaaagc tgccgtactt
      961 ttagactatg caacaacaaa cccaaatgca atggccaatg ccatgcttca attagcaaaa
     1021 aacagtggca aatattttaa ccacgatgtt gccatgtttt atacttcaag cctaccgcat
     1081 aatagcaagg tgttgcaaaa atgtgggttc ataaaaattc cgaaaaaact caacccacgg
     1141 cctttaaact taatggcaaa aaacattaca acaactaatg atgcttattt ggctggtaaa
     1201 tggcaggtta cgcttagcga atgggatgtt ttgtaataag caaataagcc aacaaataca
     1261 aaagtttaaa actaaagctg ttattaacat tgcttgcgtt cacattgaaa attaattttc
     1321 tttgccggcc taaaaatcag aaactttaaa cacaaaatat tatga
//
LOCUS       DAOMAA010000719         1365 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_118627_length_1365_cov_2.693893, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000719 DAOMAA010000000
VERSION     DAOMAA010000719.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118627_length_1365_cov_2.693893"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="PLO59_09185"
     CDS             <1..693
                     /locus_tag="PLO59_09185"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012925443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HQV01320.1"
                     /translation="NENGFQEYSFKEIAQQMFALADAAVMSSKKDALVNMGGFLTLRN
                     KTLADACTQLLIITEGYTTYGGLSGRDMEAIAVGLNEVFDADYLKYRITSTRYLGEKI
                     KSKGVSIVYPVGGHAVYVDAKKLFQHIPVNQYPGQALVCELYKTGGVRSVEIGSVMFG
                     KYDANRNLIPAPYELVRLAIPRRVYTQSHIDYVVEVFDEIIKNKTTVKGFQITYEPEF
                     LRHFTAHFKPID"
     gene            768..>1365
                     /locus_tag="PLO59_09190"
     CDS             768..>1365
                     /locus_tag="PLO59_09190"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015809083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HQV01321.1"
                     /translation="MPIYKQVGHIPNKRHIVFRNKNGNLLHEELVGTEGFSGLSTLAY
                     HLYPPTMVKEMGTPYSVRPTIAVEDNLQARSYLTMDALPMDDYLESRTILFVNDDLTI
                     GTAAPKQSMTDYFFKNADADELIFIHEGNGKLTTMFGSIDFAFGDYLIIPRGTVYQIK
                     FENAHNRMLIIESFSPIETPTRYRNNYGQFLEHAPFCER"
BASE COUNT          435 a          242 c          271 g          417 t
ORIGIN      
        1 aacgaaaacg gttttcagga atattcgttt aaagaaattg cgcaacaaat gtttgcatta
       61 gcagatgcag ctgtaatgag ttcgaaaaaa gatgctttgg taaacatggg cgggttttta
      121 acgttgcgca ataaaaccct ggccgatgct tgtacgcaac tgcttattat taccgaaggc
      181 tatactactt atggaggctt atccggacgt gatatggaag caattgcagt ggggcttaac
      241 gaagtgtttg atgctgatta tctgaaatat cgtataacca gtacccggta tctgggcgaa
      301 aaaattaaaa gcaaaggcgt ttcaatagtt tatccggttg gaggccatgc ggtttatgtt
      361 gatgcaaaaa agttgtttca acatattcct gtaaatcaat atccggggca agcattggtt
      421 tgcgaattat ataaaacagg aggagtcagg agtgtagaaa ttggatcggt aatgtttgga
      481 aaatacgatg ctaatagaaa cttaatacct gcaccttatg agttagttcg tttggctata
      541 cccagacgtg tttatacaca gtcgcatata gattatgtgg tagaagtatt tgatgagatt
      601 ataaaaaata aaaccactgt aaaaggattt caaattacat acgaacctga attcttacgt
      661 cactttactg ctcattttaa accaattgac taaaatcata aatgtaactg attatactca
      721 tttacatagt tacatattct gattgcattt gcataaaata ttaatctatg cccatttata
      781 aacaagttgg tcatataccc aacaaacgtc atattgtatt ccgaaataaa aacggcaacc
      841 ttttgcatga agaactggtt ggtaccgaag ggttttctgg cctttcaacc ttagcatatc
      901 atctgtatcc acctaccatg gttaaagaga tgggtacacc ttattctgtt cgaccaacca
      961 ttgccgttga agacaatttg caggcacgaa gttatttaac aatggatgcg ttgccaatgg
     1021 atgactattt ggaaagccgc accatcttat ttgtaaacga tgatttaacc ataggtacag
     1081 cagctcccaa acaaagcatg actgattatt tttttaaaaa tgccgatgcc gatgagttaa
     1141 tatttatcca cgaaggcaat ggtaaactta caaccatgtt tggctccatt gattttgctt
     1201 ttggagatta tttaattatt ccgcgaggta ccgtgtacca aataaagttt gagaatgccc
     1261 ataaccgaat gctcattatc gaatcgttta gtccgattga aactccaaca cgttatcgta
     1321 ataattacgg tcagttttta gagcatgcac cgttttgcga acgcg
//
LOCUS       DAOMAA010000720         1365 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_118687_length_1365_cov_1.916794, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000720 DAOMAA010000000
VERSION     DAOMAA010000720.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118687_length_1365_cov_1.916794"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="PLO59_09195"
     CDS             <1..655
                     /locus_tag="PLO59_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Fe-S-binding domain-containing protein"
                     /protein_id="HQV01322.1"
                     /translation="DQAGECHLQDLSYENGNAGTRYEFARRTFDMIDIGDKIKLHMNR
                     CILCYRCVKTAEQITDGRVHGVISRGDHAEISTYIEQAIDNDFSGNVIDVCPVGALTD
                     RTFRFKSRVWFTKPMDAHRKCDKCCGKTAVWLSGNDVLRVTARKDKFGEVEDWICNEC
                     RYDKKQIGDWTIEGPRKIERHSVISQNHYESLRQLKVDISNQKKLLEVAHVADIKQN"
     gene            686..>1365
                     /locus_tag="PLO59_09200"
     CDS             686..>1365
                     /locus_tag="PLO59_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HQV01323.1"
                     /translation="MELFDLLIKLALVVAVFTIALVIAMYSTYAERKVAAFLQDRLGP
                     NRAGPYGILQPLADGGKMFMKEEIIPSKADTFLFILGPCIAMMTACMTGVVIPWGNTL
                     IIDGKAYIMQVTDINVGILFAFAVVSIGVYGIMIGGWASNNKYSLLGAIRASSQMISY
                     ELAMGFSIIALIMTTGSLSLRDIVEQQSGFHWNFLYQPLGCLLFIICAFAETNRAPFD
                     LPECETELV"
BASE COUNT          434 a          231 c          298 g          402 t
ORIGIN      
        1 tgatcaggca ggcgaatgtc atttacaaga tttaagctat gaaaacggaa atgcaggcac
       61 gcgttatgaa tttgcccgca gaacatttga catgattgat ataggcgata aaataaaact
      121 gcacatgaat cgctgcattt tatgctatcg ttgcgtaaaa acagccgaac aaattaccga
      181 tggcagagta catggtgtaa taagccgtgg cgaccatgcc gaaatatcaa cctatataga
      241 acaagcaatt gataatgact tttcgggcaa tgtaattgat gtatgccctg ttggtgcttt
      301 aaccgaccgt acttttaggt ttaagtcgcg tgtttggttt acaaaaccaa tggatgcaca
      361 tagaaaatgc gataagtgtt gcggtaaaac agcagtttgg cttagcggta atgacgtttt
      421 acgagtaact gcacgtaaag ataaatttgg cgaagttgaa gattggattt gtaacgaatg
      481 ccgttacgat aaaaaacaaa ttggcgattg gactattgaa gggcctcgta aaattgagcg
      541 acactcggta ataagtcaaa atcattacga aagtttacgt caactaaaag ttgatatctc
      601 aaaccaaaaa aaattactcg aggtagcaca cgttgccgac ataaaacaaa actaaaagcc
      661 atacccgata accgtaatag catacatgga attatttgat ttattaataa aattagcttt
      721 ggtagtggca gtgtttacaa ttgcattggt aattgctatg tactcgacct atgccgagcg
      781 caaagtagct gcatttttac aagaccgatt aggaccaaac agggcagggc cttatggtat
      841 tttacaaccg ttagccgatg gtggtaaaat gtttatgaag gaagaaataa ttccgtcaaa
      901 ggcagataca ttcctcttta ttttaggccc ttgtatagcc atgatgaccg cttgtatgac
      961 tggtgtggta ataccttggg gtaacacttt aattatagat ggcaaagcgt atataatgca
     1021 ggttaccgat ataaatgtag gaatattgtt tgcatttgcc gttgtatcta ttggtgtata
     1081 tggcataatg ataggtggct gggcatctaa taataagtac tcattgctgg gcgctatacg
     1141 agcaagttca caaatgataa gttacgagtt ggcaatgggc ttttcaatta ttgcattaat
     1201 tatgacaaca ggctcgttaa gtttacgcga tattgtggaa caacaaagtg gtttccattg
     1261 gaacttttta taccaacctt taggttgcct gctatttatt atttgcgctt ttgctgaaac
     1321 caatcgtgca ccttttgatt tgcccgaatg cgaaaccgaa ttagt
//
LOCUS       DAOMAA010000721         1363 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_118974_length_1363_cov_2.597095, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000721 DAOMAA010000000
VERSION     DAOMAA010000721.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118974_length_1363_cov_2.597095"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1158)
                     /locus_tag="PLO59_09205"
     CDS             complement(<1..1158)
                     /locus_tag="PLO59_09205"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HQV01324.1"
                     /translation="MKDSFQITGGTQLKGSITPQGAKNEALQIISAVLLTDKLVTIHN
                     IPAIVDVIKLIDLLAQMGVSVNKIGNNSYTFQADNVQLGFLESKTFAKLASALRGSIM
                     IVGPLLARFGKANIPKPGGDKIGRRRLDTHFIGFQKLGATFNFDASDNFFSVHATKLQ
                     GTYMLLDEASVTGTANIVMAAVLAQGTTTIYNAACEPYLQQLCKMLNSMGAKISGIGS
                     NLLNIEGVKSLQGCTHTMLPDMIEIGSFIGLAAMTQSEITIKNVGLQHLGLIPATFQR
                     LGIKMEFIDDDIYIPAQERYEIDTFIDGSILTIADAIWPGLTPDLLSVLLVTATQAQG
                     TVLIHQKMFESRLFFVDKLIDMGAQIILCDPHRATVIGLNRKYPLRGIQMTS"
BASE COUNT          386 a          272 c          260 g          445 t
ORIGIN      
        1 cgatgtcatt tgaataccac gtagcgggta tttacggttt aggccgatta cggttgcacg
       61 atgtgggtcg catagaatta tttgtgcacc catatcaata agtttatcta caaaaaacaa
      121 tcggctttcg aacatctttt ggtgtatcag tacagtaccc tgtgcttgtg tggcagtaac
      181 taatagtaca cttaatagat caggcgtgag tccgggccaa attgcatcgg caatggtaag
      241 tatcgagcca tctatgaaag tgtcaatttc atagcgttct tgtgcaggta tgtagatatc
      301 atcgtcaata aattccattt taatacctaa ccgttgaaac gttgccggta ttaagcccaa
      361 atgttgcagg cccacatttt ttatagtaat ttctgattgc gtcatggccg ctaatccaat
      421 aaaactgcct atttcaatca tatcgggcag catggtatgt gtacaaccct gtaaactttt
      481 tacgccttct atgtttaata agttagagcc aataccactt atttttgcgc ccatactatt
      541 tagcattttg cagagttgct gcaagtaagg ttcgcatgct gcattgtaaa tggttgtggt
      601 gccttgcgct aaaactgcag ccattacaat gttggcagta ccggttaccg atgcttcgtc
      661 aagcagcatg taggtgcctt gtagtttagt tgcatgcact gaaaaaaaat tatccgatgc
      721 atcgaaatta aaggttgctc ctaatttctg aaaaccgata aagtgggtgt ctaatcgtct
      781 tcggccaatt ttatcgccac ccggtttggg gatgtttgct ttgccaaaac gcgctagtag
      841 cgggccaact atcataatac tgccccgtaa tgccgatgct aactttgcaa atgttttcga
      901 ttctaaaaat cccagttgca cattatctgc ctgaaacgta tagctgttgt tgccaatttt
      961 attaacgctt actcccattt gtgccaacaa atcaatcagc ttaattacat caacaatggc
     1021 cggaatatta tggatggtta ccaatttatc ggttaacaaa acagcgctta taatttgtaa
     1081 tgcttcattt tttgcaccct gcggtgttat gctgccttta agttgggtgc cacctgttat
     1141 ttgaaaacta tctttcatac acttagttta tttcgacaaa cttatttcta tacgggtttg
     1201 aatacatact gtttttttga ttttataaag aagctattaa cagcgctttg taaaaggacg
     1261 gctgcttaac attgctattc aatcttacag aaaaattgca gcataaatca aaaaaaagaa
     1321 ttatcaaaat attgcccaag gcatcgttat agtttaccta tat
//
LOCUS       DAOMAA010000722         1362 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_119159_length_1362_cov_2.538638, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000722 DAOMAA010000000
VERSION     DAOMAA010000722.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119159_length_1362_cov_2.538638"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..>1362
                     /locus_tag="PLO59_09210"
     CDS             68..>1362
                     /locus_tag="PLO59_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01325.1"
                     /translation="MNYGIYVSNALPASAVRINDNDFVNCNRAVYFEGSLNTSVTNNR
                     INVGAGFSSQQYLPYGIYSNYSSGYDVSNNIIKSTNGTYNTNLATGICINGSNAVTNR
                     LYRNTMNNVNVGTTVYGDNQGTNIGDGLSILCNSYGQGSVGLNQTDINMGAISFTFGK
                     INVMQGSTALGANNLFSHNATPLTTCIKNDFLDKGPGCYPSSNLANTFNYFFNPAPAN
                     LTKPLYYSTSLLVKPPIASSYDVNAMCPVVFGGSSGGGTFSHKSVAAAKQTIVITTAS
                     VSALLAMVDGGNTQTLLTAINSQSPGNLKNLLESKSPYLSDAVLMAYFAKTSTPPGHV
                     KEIHDKNKPVSEDVWQVILNRNLPNGIMNQLNNQQSVTTINQLVKLQAQVSDLKQAKG
                     FIIDETIRQLMADSVNGWSADSIIDLMDADNRYLANQKL"
BASE COUNT          452 a          232 c          249 g          429 t
ORIGIN      
        1 ttgttcagca tacggcgtta atggctgcac aaactattca actaataatc catcaacttt
       61 tacgaacttg aattatggta tttatgtctc taatgcttta cctgcttctg ctgttagaat
      121 taacgataat gattttgtga actgtaatcg cgcagtttat tttgaaggct cattaaatac
      181 atcagtaaca aataaccgca tcaatgttgg tgcaggtttt tcatcacagc aatatttgcc
      241 ttacggtatt tattctaatt attcatctgg ttacgatgtt tcaaataaca ttattaaatc
      301 aactaatgga acttataata caaatttagc aactggtatt tgcattaacg gttcaaatgc
      361 agttacaaat agattatacc gtaatacgat gaataacgta aacgttggca caacggtata
      421 tggcgataat caaggaacaa atattggtga tggtttaagt atactatgta atagctatgg
      481 acaaggatct gtaggcttaa atcaaacaga tataaatatg ggagcaattt cttttacttt
      541 tggtaaaatt aatgtaatgc aaggaagcac tgcgctaggt gctaataatt tattttcgca
      601 taatgcaacc ccacttacta cttgtattaa aaacgatttt ttagataagg ggcctggttg
      661 ctacccttcg agtaacttag ctaatacctt taattacttt tttaacccag caccagctaa
      721 cctaacaaag cctttgtatt atagtacttc gttacttgta aagccaccta tcgcaagctc
      781 ttatgatgtg aatgcaatgt gtccagttgt ttttggcggc agttctggcg gcggcacgtt
      841 ttcgcataaa tctgtagctg ctgctaaaca aactattgtt attacaacag ccagcgttag
      901 tgccttatta gcaatggttg atggtggtaa tactcaaact ttattaactg ctattaatag
      961 ccaaagcccc ggtaacctaa aaaatttatt ggaaagcaag agcccttatt taagtgatgc
     1021 agttttgatg gcttactttg caaaaacgag cacgccaccg ggtcatgtaa aagaaataca
     1081 tgataaaaat aaaccagtga gcgaggatgt gtggcaggtt attttaaacc gtaatttacc
     1141 aaatggtata atgaatcaat tgaataatca gcaaagcgta acaactatta accaattggt
     1201 aaaactgcaa gcccaagtaa gtgatttaaa acaagcaaag ggctttataa ttgatgaaac
     1261 catcaggcaa ttgatggccg atagtgtaaa tggttggagt gcagatagta tcattgatct
     1321 aatggatgct gataaccgat atttagccaa tcaaaaacta tt
//
LOCUS       DAOMAA010000723         1362 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_119167_length_1362_cov_2.478194, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000723 DAOMAA010000000
VERSION     DAOMAA010000723.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119167_length_1362_cov_2.478194"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            235..525
                     /locus_tag="PLO59_09215"
     CDS             235..525
                     /locus_tag="PLO59_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016779071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HQV01326.1"
                     /translation="MTKSDLVNEIHERTGIEKTQIVATVEAWMKLIKNNLGEQKAVYL
                     RGFGTFYAKKRAAKMGRVITRNEQVFIPEHHIPAFKPAKSFMSKVKNAYTQK"
     gene            643..>1362
                     /locus_tag="PLO59_09220"
     CDS             643..>1362
                     /locus_tag="PLO59_09220"
                     /inference="COORDINATES: protein motif:HMM:NF037626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HQV01327.1"
                     /translation="MKNALINKFKNHKHFSPLLTAISTNQKQPVCFEGLRGSSISVLI
                     AALYQTQPFNAVFILNDKEEAAYFANDLENLLGEKKIFFLPVSYKKFNDFETLDNQLV
                     LQRAEILNHISKHNQGILLVTYSQAITEKVTTKRSLQQNTFDVKRNEKLSIEFLSEFL
                     DSNAFEQTDFVVEAGQYAVRGGIMDVFSFAYELPFRIEFGDDVIESIRTFDPTNQLSV
                     QPMDYVSIIPNVKTKLLKESKE"
BASE COUNT          488 a          240 c          218 g          416 t
ORIGIN      
        1 ttacgtttgt ttatttgata ccaaaaaatt aatgaatcct gaatgcgttt aaaatcgtca
       61 ttctttattt ttacacccct caaaatataa tagttttttt aattttaaaa cacattaaaa
      121 acaccttatt acaaaggtat tcagattaaa ttatgtaaat tgcagtggca taattcccat
      181 gcgcaatatc cataaataca ctattaaatt tcaatatatt ttagataatt agttatgaca
      241 aaatcagatt tagtaaacga aattcacgaa cgtacaggca tcgaaaaaac acaaattgta
      301 gctaccgttg aggcctggat gaaacttatt aaaaacaatt tgggcgaaca aaaagcagtg
      361 tacttgagag gctttggtac cttttatgca aaaaagcgtg cagcaaaaat gggtcgcgtt
      421 attacacgca atgagcaagt gtttatcccc gaacatcaca taccggcatt taaacctgca
      481 aagtcattta tgtcgaaggt aaaaaacgca tacacccaaa agtaaactga accggattat
      541 ttaaacccat tgcgtaatat cattaagcag ctcaagcaat tttagtttag ctataatttt
      601 aaaaccccgg caaaccatgt tggggtacct atttaattta ttgtgaaaaa cgccttaatt
      661 aataaattta aaaatcacaa acatttttcc ccgctgttaa cagctatatc aaccaatcaa
      721 aagcagcctg tttgttttga aggacttaga ggttcttcta tcagcgtttt aatagcagcc
      781 ttatatcaaa cgcagccatt taatgcagtt tttattttaa atgataagga agaagctgcc
      841 tactttgcaa atgatttaga aaacttatta ggcgaaaaga aaattttttt tctgcccgtt
      901 tcgtataaaa aatttaatga tttcgaaaca ttagataatc aactcgtatt acaacgtgcc
      961 gaaattttaa accatatcag caaacacaat cagggtattt tattagttac ttacagccaa
     1021 gccataaccg aaaaagttac taccaaacgc agcttgcaac aaaacacttt cgatgtaaaa
     1081 cgaaatgaaa aactaagcat agaattttta tcagagtttt tagacagtaa tgcattcgaa
     1141 caaaccgact ttgttgttga agcgggacaa tatgcagtgc gcggtggtat tatggatgtg
     1201 ttttcatttg catacgaatt gccttttcgt attgagtttg gcgatgatgt tattgaaagc
     1261 atccgaacct ttgacccgac caatcagcta tcggtgcaac cgatggatta tgttagcatt
     1321 atacccaatg ttaaaaccaa gttgctcaaa gaaagtaaag ag
//
LOCUS       DAOMAA010000724         1361 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_119327_length_1361_cov_2.401991, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000724 DAOMAA010000000
VERSION     DAOMAA010000724.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119327_length_1361_cov_2.401991"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="PLO59_09225"
     CDS             complement(<1..526)
                     /locus_tag="PLO59_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01328.1"
                     /translation="MEIRILLFNSNLKYLDALSALITSVQCFKLVGAFLKIDDALNRT
                     VNLQPNVILIDNGYAPQVYINTINLLQQNLPDLKILPVINTHRGFDRHINQNIKEGVC
                     VLTGMPPAKIVTTIVKLVLSSKNGKLKTIELTNSQQHLLAPTLLNKKEYVPTNKIKKW
                     VMALIFKCKCANDWV"
     gene            complement(602..>1361)
                     /locus_tag="PLO59_09230"
     CDS             complement(602..>1361)
                     /locus_tag="PLO59_09230"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HQV01329.1"
                     /translation="NEQDLQRATLKAEKQLRFIMLGIAVILLLAAIMFFNRYRFKQKL
                     AQQHALINQRKHISADLHDDIGATLSSINIYSEAIKNKLKQNETNKVMDLVDKIGDNA
                     RDTITSLSDMVWSINPSNDAANMLFERMQNFAIPLLASKNIDMEFEYNQSLQQQLFSM
                     EVKQNVFLIFKEIVNNIAKYSKASQVLVRIESANPFMVLKIIENGVGFDTSKTYAGNG
                     LRNMKLRANEINCNLSCQSTAGFTQYILDIPLAD"
BASE COUNT          404 a          240 c          213 g          504 t
ORIGIN      
        1 ctacccaatc atttgcacat ttgcacttaa aaattaatgc cataacccat ttttttattt
       61 tgttggttgg cacatattct tttttgttta aaagcgttgg cgctaataaa tgttgttggc
      121 tatttgttag ttcaatagtt ttaagtttac catttttact cgatagcacc agcttaacta
      181 tggtagttac tattttggca ggtggcatac cagttaaaac acaaacacct tcttttatat
      241 tttgattaat atgcctatca aaacctctat gtgtatttat aaccggcaaa atttttaaat
      301 cgggcaaatt ttgctgcaat agattaatgg tgttaatata tacctggggt gcataaccat
      361 tatctattaa tatcacattg ggttgcaaat ttaccgtgcg atttaaggca tcatcaattt
      421 ttaaaaatgc acctaccaat ttgaaacatt gaacagatgt aattaatgca cttaatgcat
      481 ctaaatattt taggttgctg ttaaacaaaa gtatcctgat ttccatagta ccataattta
      541 aagcacaaaa atagtggcat gtgtatgata ccctatcccc taattcggtg atttttatgc
      601 tttaatcagc caaaggtata tctaagatat attgtgtaaa tccagcagta ctttgacaac
      661 ttaagttgca gttaatttcg ttagcgcgaa gcttcatatt tcttaaccca ttgcctgcat
      721 acgtctttga tgtatcaaaa cctactccgt tttcgataat ttttagtacc atgaatgggt
      781 tagcagattc aatacgcacc aacacctgac ttgctttaga atatttggca atgttgttta
      841 caatttcttt gaaaattaaa aatacatttt gcttcacttc catcgaaaac aactgttgtt
      901 gtaatgattg attgtattca aactccatat caatattttt tgaagccaaa agtgggatgg
      961 caaaattttg catcctttca aacaacatat tggctgcatc gttgctcgga ttaatactcc
     1021 acaccatatc acttaagctt gtaatggtgt cgcgtgcatt atcgccaatt ttatcaacca
     1081 aatccattac cttattggtt tcattttgtt taagtttatt ttttatggct tcactgtata
     1141 tgtttatgct gctaagtgtg gcacctatat cgtcatgcaa atcggcactg atgtgtttac
     1201 gctgattaat taatgcatgt tgctgggcta atttttgttt gaagcggtat cggttaaaaa
     1261 acataattgc tgccaacagc aatattacag caatacctaa cattataaat cgtaattgtt
     1321 tttcagcctt tagggttgca cgttgtaaat cttgttcgtt t
//
LOCUS       DAOMAA010000725         1360 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_119414_length_1360_cov_3.527969, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000725 DAOMAA010000000
VERSION     DAOMAA010000725.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119414_length_1360_cov_3.527969"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1145)
                     /gene="coaBC"
                     /locus_tag="PLO59_09235"
     CDS             complement(<1..1145)
                     /gene="coaBC"
                     /locus_tag="PLO59_09235"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013622850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HQV01330.1"
                     /translation="MLKDKKIILAVTGSIAAYKTPQLVRTLVKAGAHVKVIVTNDALQ
                     FVTTLSLATVSKNDVVTDLVKSDGQWQNHVSWGLWADALLIAPATANTIAKMANGICD
                     NFLLATYLSARCPVFVAPAMDVDMYKHQSTQQNIAVLKARGTYILGPDDGELASGLHG
                     FGRMLDEDSLTKALGLFFINNKKLCGLNIVITAGPTREAIDPVRYISNHSTGKMGVAL
                     ADAAFNMGAEVTLILGKGALPINNKHYKVVYVDTAAQMLETSLLNFEKANVFIMAAAV
                     ADYKPAAPSNQKIKKKNADLNLLMEPTTDVLATLGKQKKNQTLVGFALETDNELENAK
                     SKLKRKNLDFIVLNSMRDNGAGFATDTNKVTIIDTKGTILSLELKPKS"
     gene            complement(1227..>1360)
                     /locus_tag="PLO59_09240"
     CDS             complement(1227..>1360)
                     /locus_tag="PLO59_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase Rpb6"
                     /protein_id="HQV01331.1"
                     /translation="EVFENREQIEISRHFERMPKPTLVALKEYVEGKVYARNPGKID"
BASE COUNT          377 a          285 c          235 g          463 t
ORIGIN      
        1 gatttaggtt tcagctctaa cgataaaatt gtaccttttg tatcaataat ggttacttta
       61 ttggtatcag ttgcaaagcc tgcaccgtta tcgcgcatcg agttaagcac tataaaatct
      121 aagtttttgc gcttcagttt cgattttgca ttttcaagtt cattatcggt ttcgagtgca
      181 aagccaacca atgtttgatt tttcttttgt ttaccaagtg ttgccaatac atcggtagtt
      241 ggttccatca ataaattaag gtcagcattt ttctttttaa ttttttgatt gcttggcgct
      301 gccggtttgt aatcggcaac agccgctgcc ataataaaca cattggcttt ttcgaagttt
      361 aataaacttg tttcgagcat ttgcgctgcc gtgtcaacat ataccacttt gtaatgcttg
      421 ttattaatgg gcaatgcacc tttgcctaaa ataagcgtta cctcagcacc catattaaaa
      481 gcagcatcgg ccaaagctac acccattttg ccggttgagt ggttgcttat ataccgcacc
      541 ggatcaatgg cttcgcgtgt aggcccggca gttataacta tatttaaacc acaaagcttt
      601 ttattattta taaaaaaaag tcctaatgct ttagttaatg aatcttcgtc taacatacgg
      661 ccaaaaccat gtaacccgct agctaattca ccatcatcag gccctaatat gtaagtacct
      721 ctggctttta atactgcaat attttgctgt gtgctttgat gtttatacat atccacatcc
      781 attgctggtg caacaaatac cggacatcgg gctgataaat atgtagccag taaaaagtta
      841 tcgcaaatgc cattagccat tttcgcaatg gtgtttgcag ttgccggtgc aattaatagt
      901 gcatcggccc atagccccca acttacatgg ttttgccatt ggccatcgct ttttaccaaa
      961 tcagtaacca catcattttt ggatacagtt gctaacgaaa gtgttgtaac aaattgaagt
     1021 gcatcgttgg taacaattac ctttacatgg gctcctgctt ttacaagtgt acgcaccaac
     1081 tgaggtgttt tgtaagctgc aatacttccg gtaactgcaa gaataatttt tttatccttt
     1141 aacatctgtt ctaattactg gcataaataa aaaatcgaaa cccatcactg cgatgaattt
     1201 cgatttattt aaaactttaa atattgttaa tcaattttgc ctgggtttcg tgcatacacc
     1261 ttaccttcaa catattcttt taatgcaact aaggtaggct taggcattct ttcgaaatgc
     1321 cgcgaaatct caatttgctc ccggttttca aacacttctt
//
LOCUS       DAOMAA010000726         1359 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_119604_length_1359_cov_3.039110, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000726 DAOMAA010000000
VERSION     DAOMAA010000726.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119604_length_1359_cov_3.039110"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /gene="miaA"
                     /locus_tag="PLO59_09245"
     CDS             <1..927
                     /gene="miaA"
                     /locus_tag="PLO59_09245"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HQV01332.1"
                     /translation="NKYNALVILGATATGKTKLATQLAYHFNGEIISADSRQVYKDMD
                     IGTGKDLEDYSINNQSVRHHLIDIVPAGSKYHIHQFKQDFSNAFLQTINNKKLPIICG
                     GSGLYLQACLFDLPFTAVPINSDLRNKLQHIEKQTLLKMLAQIPATIYTPHIDTSSSK
                     RIIRAIEINSWLQNHTLPLKVNNAVTPFVIGLQCDRAVLIQRISNRLSMRLENGLIDE
                     VARLKENGLENELQYYGLEYKFVLQYLQGFLSSDQLQDKLGTAINQFAKRQVTFFKKL
                     EREGLKINWLDAAMPQQTILNEAIDLVTPHFI"
     gene            complement(920..>1359)
                     /locus_tag="PLO59_09250"
     CDS             complement(920..>1359)
                     /locus_tag="PLO59_09250"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HQV01333.1"
                     /translation="LSNKVPHKYWKKQIRKAKYGKVLTEKLDGEFWNNGQFVISSAGQ
                     IDFLKRLNSNKLPFKNKYCQWAKKLMLIEEDKNYKLYAKTGLQELPDKNVGWFIGFIE
                     KPNGIFYFATTIESTLPVPESFSMSRVYITKKILADLGIIVSK"
BASE COUNT          458 a          234 c          230 g          437 t
ORIGIN      
        1 aataagtata atgccttagt tattttaggt gccactgcaa caggcaaaac caaattggca
       61 acacaacttg cttaccattt caatggcgaa attattagtg ccgatagccg tcaggtttat
      121 aaagatatgg atatcggtac gggtaaagat ttagaagatt atagtataaa taatcaatca
      181 gtcagacatc atctgattga tatagtaccg gccggcagca aataccacat tcatcagttt
      241 aagcaagatt tttcaaacgc ttttttgcaa actataaata ataaaaagtt acccattata
      301 tgtggtggca gtggcttata cttacaggct tgtttgtttg atttaccttt taccgctgta
      361 ccaataaaca gcgatttacg aaacaagttg cagcatattg aaaaacaaac gctgctaaaa
      421 atgttagcgc aaataccggc aactatttac acaccacaca ttgataccag cagcagtaaa
      481 agaattatac gcgcaatcga aattaattca tggttacaaa atcacacctt accacttaaa
      541 gtaaataatg cggttacacc atttgttatt ggtttgcaat gtgacagagc ggtgttaata
      601 caacgtatat ctaacaggtt atcaatgcgc ttagagaatg gtttaattga cgaagttgct
      661 cggttaaaag agaatggatt agaaaacgaa ttacagtatt acggtttaga gtacaaattt
      721 gtacttcaat atttgcaagg gttcttaagt agtgatcaat tgcaggataa acttggtaca
      781 gccataaatc agtttgccaa aaggcaggta acttttttta aaaagcttga gcgcgaagga
      841 ttaaaaataa actggcttga tgcagctatg ccacaacaaa ctattttaaa tgaggcaatt
      901 gatttagtta caccacactt tatttagaaa cgataatacc caaatcggct aaaatttttt
      961 tggttatata aaccctgctc atcgaaaacg attcgggcac aggcaatgta ctttcgatgg
     1021 tggttgcaaa ataaaatatg ccattaggtt tttcgataaa accaataaac cagcccacat
     1081 ttttatcagg taattcttgc aaacctgttt tagcataaag tttataattt ttatcttctt
     1141 caataagcat cagcttttta gcccattggc agtatttatt tttaaatggt aatttattgc
     1201 tgtttaatct ttttaaaaag tcaatttggc cggcagagct aattacaaat tgaccattgt
     1261 tccaaaattc gccatctaat ttttcggtta gcactttgcc atactttgct tttctaatct
     1321 gctttttcca atatttatga ggcactttat ttgataatt
//
LOCUS       DAOMAA010000727         1359 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_119613_length_1359_cov_2.820552, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000727 DAOMAA010000000
VERSION     DAOMAA010000727.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119613_length_1359_cov_2.820552"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1359
                     /locus_tag="PLO59_09255"
     CDS             <1..>1359
                     /locus_tag="PLO59_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS C-terminal target domain-containing
                     protein"
                     /protein_id="HQV01334.1"
                     /translation="DYIFGDETLWWVINDVGEAHTATVDGGQIGVEIRCQGFAFKTND
                     EVNNMTFYKYQIINRGTSPLLNTYFGVWCDPDLGKATDDFVGCDVSLGLGYVYNGDAD
                     DDGGGAYGQNPPAAGIDFFQGPLADPFDGIDNNKDGVIDEPGEQIIMSKYLYYNNVNN
                     TPDGNPNGAAHHYNYLQGIWGDGQVMTYGGDGRDANNPPCNYMFPGLTDPDFPSTNWT
                     EVTAGNTPEDRRFLQSAGKFTLQPGAVNYITTGVVWARTSTGGPEGSISLMKLADAKA
                     QAIFDNCFQVLDGPNAPDLAIRELSNQIIITLQNYNDSSVELYSKIDPVIPEVVYQGN
                     DTIYYSDEQRKYKFQGYILYQFKNATVTTADIGNPDKVRTVAEFQCDVADNISQLVNH
                     TYDDNLGYWIPQEKVKGANQGIKHVFNVTTDLFATDDPKLINNKQYYFTIISYAYNNF
                     APF"
BASE COUNT          422 a          239 c          288 g          410 t
ORIGIN      
        1 gattacatct ttggtgatga aacactttgg tgggtgataa atgatgtagg tgaggcgcat
       61 acagcaacgg ttgatggagg ccaaattgga gttgaaatac gttgccaagg ttttgcattc
      121 aaaaccaatg atgaagttaa caatatgacc ttttataaat accaaattat aaatcgcggt
      181 acttcgcctc ttttaaacac ctattttggt gtatggtgcg accctgattt aggaaaagcg
      241 accgatgact ttgttggatg tgacgtaagt ttaggattgg gatatgttta taacggtgat
      301 gctgatgatg atggtggagg tgcttatggt caaaacccac cagctgccgg tattgatttt
      361 tttcaaggtc ctttagcaga tccgtttgat ggcattgata ataataaaga tggtgtgatt
      421 gatgaaccag gcgagcaaat tatcatgtct aaatatcttt attacaataa cgtaaataat
      481 acaccagatg gcaacccaaa tggtgcagca catcactaca attatttgca aggaatttgg
      541 ggcgatggcc aggttatgac ttatggcggt gacggacgtg atgccaataa cccaccctgt
      601 aactatatgt tccccggttt aacagaccct gatttccctt caacaaactg gacagaggtt
      661 acggcaggca acacacctga agacagacgt ttcctgcaat cggccggtaa gtttacctta
      721 caaccgggcg cagtaaatta tattaccacc ggagtagttt gggcacgtac cagtaccgga
      781 ggtccagaag gttcgataag cttaatgaaa ttagccgatg ctaaggctca ggcgattttc
      841 gacaattgtt ttcaagtttt ggatggccct aatgcacctg atttagctat acgagagtta
      901 agtaatcaaa ttattataac acttcaaaac tataacgatt cttctgttga actttattca
      961 aaaattgacc ctgtaatacc tgaagtagtt tatcaaggta acgatactat ttattacagt
     1021 gatgaacaac gtaagtataa gtttcaggga tatattttat atcagtttaa aaatgcaact
     1081 gtaactacgg ctgatatagg taatcctgat aaagtacgta ccgttgccga gtttcaatgt
     1141 gacgttgcag ataatatttc gcagttagta aatcatactt atgatgacaa tttaggttat
     1201 tggataccac aagaaaaagt taagggggct aatcagggaa ttaaacacgt gtttaacgta
     1261 actactgatt tgtttgctac cgatgatccg aagctcataa acaataagca gtattatttt
     1321 actattattt cttatgccta caacaatttt gcaccattt
//
LOCUS       DAOMAA010000728         1356 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_120146_length_1356_cov_2.716372, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000728 DAOMAA010000000
VERSION     DAOMAA010000728.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120146_length_1356_cov_2.716372"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1044)
                     /locus_tag="PLO59_09260"
     CDS             complement(<1..1044)
                     /locus_tag="PLO59_09260"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HQV01335.1"
                     /translation="MISVPEAFDIIKQQVNKLPCHTVALPQAFNCVLAKPVFALYDTP
                     PFNNSAMDGYAFRFADLQYSPNFSVIGSVSAGSTFNGTLMKNEAVRIFTGACIPVDAD
                     TVVMQEKTIQNTHTVTVTDEEITLGSNVRLKGSQNKVNEMLVRAGNSINEMIIGYLAS
                     AGIDKVTVYQKPSVCIITTGTELIMPGLPLQPGKIFESNSWMLNAALNKMFMQTQLYA
                     CDDSMQHLSNLLAAKATETDVIIITGGVSVGDYDFVAPALKQLGAQIFFHKVKQKPGK
                     PLLFAKLNNAFIFGLPGNPASALSCFYNYVKPALHLMQGMVWPMYQTALLQNTFQKKE
                     GLTHFVKSVCNNGG"
     gene            complement(1048..>1356)
                     /locus_tag="PLO59_09265"
     CDS             complement(1048..>1356)
                     /locus_tag="PLO59_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin phosphate synthase MoaA"
                     /protein_id="HQV01336.1"
                     /translation="TSKKYIVPGHAGTFAVISTMSEPFCNSCNRLRLTADGKMKNCLF
                     SNGEVNLLQALRNGQDLLPIIKLCVQQKAKALGGQFLPEIKNVDATKISNRSMIKIGG
                     "
BASE COUNT          402 a          267 c          239 g          448 t
ORIGIN      
        1 gccaccattg ttgcaaacac ttttaacaaa atgcgttaac ccttcttttt tttgaaatgt
       61 gttttgaagt aaagcagttt gatacattgg ccaaaccata ccttgcatta aatgtaaagc
      121 cggttttacg taattgtaaa agcaactcaa agccgatgcc ggatttccag gtaagccaaa
      181 aataaatgca ttgtttaatt tggcaaacaa taaaggttta cctggttttt gctttacctt
      241 atgaaaaaat atttgtgccc ccaattgttt taatgcaggt gctacaaaat cataatcgcc
      301 aaccgataca ccacccgtaa taattatcac atccgtttca gttgctttag cagctaataa
      361 attacttaaa tgttgcatgc tgtcgtcaca agcgtataac tgcgtttgca taaacatttt
      421 attgagtgca gcgtttaaca tccaactgtt cgattcgaaa attttaccgg gttgtaaagg
      481 caagcccggc ataattaatt cggtaccggt agtaataata catactgatg gcttttggta
      541 aacggtaact ttgtcaatac ctgccgaagc taaatagcct ataatcattt cgtttatcga
      601 attacctgca cgaaccagca tttcgtttac tttattttga gagcctttca accgaacatt
      661 actgcccaat gttatttctt catcagttac agttactgta tgtgtatttt gtatggtttt
      721 ttcttgcatt acaacggtat cggcatcaac aggtatgcag gccccggtaa aaattcgtac
      781 ggcttcgttt ttcatcaaag tgccattaaa tgtactgcct gccgaaacgc tgcctattac
      841 agaaaaattg gggctatatt gtaaatccgc aaatcggaat gcataaccat ccatggccga
      901 gttgttaaat ggtggtgtat cgtataaagc aaaaacaggt ttagccaata cacaattaaa
      961 agcctgtggc aacgccacag tatggcatgg cagtttattt acttgttgtt taataatatc
     1021 aaaggcttcg ggtactgata tcataattta acctccaatt ttaatcatac ttctgttgct
     1081 gatttttgta gcatctacat tttttatttc aggtaaaaac tggccgccta aagctttggc
     1141 tttttgttgt acacatagtt ttataatagg caataaatcc tggccatttc taagtgcttg
     1201 caacaaattt acttcaccgt tagaaaacaa acagtttttc attttaccat cagcggttaa
     1261 gcgcaatcgg ttgcaactat tacaaaatgg ttcgctcatg gtgcttataa ctgcaaaggt
     1321 gcctgcatgg cccggtacta tatatttttt agacgt
//
LOCUS       DAOMAA010000729         1356 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_120150_length_1356_cov_2.636434, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000729 DAOMAA010000000
VERSION     DAOMAA010000729.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120150_length_1356_cov_2.636434"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="PLO59_09270"
     CDS             complement(<1..849)
                     /locus_tag="PLO59_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HQV01337.1"
                     /translation="MIQLQNYACGQWVAGTQNGQTLYNAITGQAVATASSSGLDFKSM
                     LNHARTVGGPALRKLTFHERGRMLKALALYLNERKESFYKISWATGATRADSWVDIEG
                     GFGNLFTYASLRRQFPNETYCLDGDVAKLSKEGTFLGNHICVPLEGVAIHINAFNFPV
                     WGMLEKIAVNLFAGVPAIVKPATVTSFLTEAVFKEIIASNILPQGALQLICGSAQGIV
                     DLVQAGDVVTFTGSAHTGQLLKKNPALAENSVPFNLEADSLNCCILGLDAAPGTANFE
                     VFIKEIH"
     gene            complement(882..>1356)
                     /locus_tag="PLO59_09275"
     CDS             complement(882..>1356)
                     /locus_tag="PLO59_09275"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HQV01338.1"
                     /translation="YSFEIPHQVVAGGQWRAQSVHFGLQHWAACTGIVAVHDAARPLA
                     SKALIALLYNEAEVYGNAIPFLPLTESIRQTDGNTNRAVNRNNYVAVQTPQCFDLQQL
                     LSAYNEVVSYDFTDEASLFENKATIHLVPGQSNNIKITKPGDIAWAQQFLQQQNI"
BASE COUNT          358 a          300 c          288 g          410 t
ORIGIN      
        1 gtgaatttct tttataaata cctcaaaatt agctgtgccg ggtgcggcat ccaaacctaa
       61 aatgcagcag tttaacgagt cggcttcgag gttaaagggt acactgtttt cggctaaggc
      121 cggatttttt ttaagtaact gacctgtatg cgcgcttccg gtaaaggtaa ccacatcgcc
      181 tgcctgcacc aaatcaacaa taccttgtgc cgaaccacat ataagttgca atgcgccttg
      241 tggtaaaatg ttgctggcaa taatttcttt aaaaacggct tcggttaaaa acgatgtaac
      301 ggtagcaggt tttactatgg ccggcacccc tgcaaaaagg tttactgcaa ttttttcgag
      361 cataccccat accggaaaat taaatgcatt gatatgtatg gccacacctt ctaatggtac
      421 acaaatatga tttcccaaaa aggtaccttc tttactgagt ttagccacat cgccatctaa
      481 acaataggtt tcgttcggaa actgcctgcg taaacttgca taggtaaata agttgccaaa
      541 accaccttca atatcaaccc agctatcggc acgggttgca cctgtagccc agctaatttt
      601 ataaaacgat tctttacgct catttaaata tagggccaat gctttaagca tacgcccgcg
      661 ctcatggaat gtaagtttac gcaatgccgg tccgcctaca gtacgtgcat ggttaagcat
      721 ggatttaaag tctaacccac tactcgatgc tgtggctacg gcctggcctg taattgcatt
      781 atataatgtt tggccgtttt gggtacctgc tacccattgg ccgcaggcat agttttgtaa
      841 ttgtatcatt tgttaagtta atgtcggtta ccggttttat tttaaatatt ttgttgttgc
      901 aaaaactgct gtgcccatgc tatatcaccg ggcttggtaa ttttaatatt gttgctttgg
      961 cctggcacca aatgtatggt tgccttattt tcgaatagcg atgcttcgtc tgtaaagtcg
     1021 tagctaacca cttcgttata cgcacttaaa agttgctgta aatcgaaaca ttgtggtgtt
     1081 tgcactgcca cataattgtt gcggtttacg gcccggtttg tattgccatc ggtttggcgt
     1141 attgattcgg ttagcggtaa aaaaggtatg gcattgccat aaacttctgc ttcattataa
     1201 agcaacgcta ttaatgcttt agaggccaat ggccgggctg catcgtgcac agccacaatg
     1261 ccggtgcatg cagcccaatg ctgtaagcca aagtgcacac tttgtgcacg ccattggcca
     1321 ccggcaacta cctgatgtgg tatttcaaaa ctgtat
//
LOCUS       DAOMAA010000730         1356 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_120153_length_1356_cov_2.562644, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000730 DAOMAA010000000
VERSION     DAOMAA010000730.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120153_length_1356_cov_2.562644"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            194..273
                     /locus_tag="PLO59_09280"
     tRNA            194..273
                     /locus_tag="PLO59_09280"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:228..230,aa:Leu,seq:gag)
     gene            complement(353..>1356)
                     /locus_tag="PLO59_09285"
     CDS             complement(353..>1356)
                     /locus_tag="PLO59_09285"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HQV01339.1"
                     /translation="IIVDDNGSKPAMLKGLHIATKWRHELDQKALNNPDVTSVTLYGW
                     WMDMNPQKGVYDWSRLDGELTRFTNAGKNIGLMLAGGMRCPAWIFDEGVRHFTIVEFK
                     HGGKGKEFSHEQPVVYDRAYIDNFNTFVKALAAHMKTMPYWNKLTHVSITGVNRTTAE
                     FRLPAQSNMEVDGVTSTDATDLWKKNGYSATVVLKAFEEITNVVATAFPNRYLIIPII
                     NNTASAFPDIDKVNIPTEGLKWLKQKYPNHAAAQYTALTPNFNGLGFVQTVREMGIPI
                     GYELNESKNRLNEDEKFFKDGVQKGIDAKALYIEIFPENAIRFTQATTTLSPKINMQ"
BASE COUNT          382 a          266 c          250 g          458 t
ORIGIN      
        1 taagtaaatc atcaggtttg cttttgccac acgtgtttac atataacaaa gctattagcg
       61 ccattacaat gagtaaccaa attatgtttt gtttttgttc catgtgtttg tgtgaagata
      121 aatattttgt attgaaaata tagtcaacaa attttcagac cggtttaaat attgtagttt
      181 tggcggcttc aatgcccggg tggcgaaatt ggtagacgca ccatcttgag ggggtggcgt
      241 gcaaacgtac gagttcgaat ctcgtctcgg gcacctaaat attaaaataa aaaaagccga
      301 aacactttta cgtttcggct ttttttatgg gcttaaaaat gatttttaaa aactattgca
      361 tatttatttt agggcttaat gtggtagtag cttgcgtaaa acgtatggca ttttccggaa
      421 atatttcgat atacaaagcc ttggcatcaa tacctttttg aacgccatct ttaaaaaact
      481 tttcatcttc gtttaaacgg tttttacttt cgtttaattc ataaccaatt ggaatgccca
      541 tttcgcgtac tgtttgcaca aatcccaatc cattaaagtt tggtgtaagt gctgtgtatt
      601 gtgcagcagc atggttgggg tatttttgtt tgagccactt taaaccttcg gttggtatat
      661 ttactttatc aatatccgga aaagcacttg ctgtattatt tatgattgga attatcaaat
      721 aacgatttgg gaaagcagtt gcaacaacgt tggttatctc ttcaaatgct tttaaaacta
      781 cagtagcact atatccattt tttttccaaa gatcagttgc atcggtactg gttacgccat
      841 caacctccat attactttgt gcaggcaacc taaattctgc agtggtgcgg ttaacacctg
      901 ttatcgaaac gtgcgttagt ttattccagt aaggcatggt tttcatgtga gcagccaggg
      961 cttttacaaa cgtgttgaag ttatcaatgt aagctctgtc ataaactacc ggttgctcat
     1021 gcgaaaactc tttgcctttg ccaccatgct taaactctac aatggtaaag tgtcgcacac
     1081 cttcatcaaa aatccaggcc gggcaacgca taccaccggc caacattaaa ccaatatttt
     1141 tgcctgcatt tgtaaagcgt gttaactctc catctaaacg actccaatcg taaacaccct
     1201 tttgtggatt catatccatc caccatccat aaagcgttac agatgtaaca tccggattgt
     1261 ttaatgcctt ttgatctaac tcatgacgcc attttgttgc aatatgtaag cctttaagca
     1321 ttgcgggttt gctgccgtta tcatcaacaa ttatag
//
LOCUS       DAOMAA010000731         1355 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_120349_length_1355_cov_2.300000, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000731 DAOMAA010000000
VERSION     DAOMAA010000731.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120349_length_1355_cov_2.300000"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..>1355)
                     /locus_tag="PLO59_09290"
     CDS             complement(115..>1355)
                     /locus_tag="PLO59_09290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HQV01340.1"
                     /translation="GGNGIFYILQLHFWCKLFGATDVAVRMLSALYGIALLFSVYYIA
                     KKKFKIPALAIGATAVAAFHPLLIMYAREARPYAAATFYTIWSSYFLFHCFFSYQPNS
                     KPHVVNILFYIVATLAALLCHYLTAYIFITQGLWVIIYLRKRIRIFYFLVAVSLAATG
                     LSLWMYVAGLKGMAVMHAHNNYYVTELSKNSDFVQPTTFLNCIIGSIQFALPALGNSL
                     QKSGLQLREIILLLLIPLHLIYVAWRKADKSIRKYLNYCILSLPVAVLFITALAINAG
                     HTISYQPHYANFVAAQLVFIMGVGCVYAFWQHKNVVLNRLVIISFALILSGTISFYFL
                     KPLNVYSNDVKNPYQFIALKISETSKTDTIYFPNITDAQLAYMYLKPTERIFSINASS
                     TCVYINDKQQTILDLNKLRY"
BASE COUNT          492 a          217 c          241 g          405 t
ORIGIN      
        1 catatagatt tgaaaatttt atggccataa ggcccatttt gaatggatgc gaatttaata
       61 tttgattgac ttaaatttta attgtattaa tgtttcgatt atgttattta aaaattaata
      121 acgcaattta ttcaagtcta aaattgtttg ctgtttatca ttaatgtaaa cgcatgttga
      181 acttgcatta attgaaaaaa tacgttcggt aggtttaaga tacatgtatg ctaattgtgc
      241 atcggtgata ttgggaaagt atatcgtatc ggtttttgaa gtttcgctaa tttttaaagc
      301 gataaattga tatgggtttt ttacatcgtt actataaacg tttaatggtt ttaaaaagta
      361 aaagctaata gtgccactta aaataagcgc aaaacttata attaccaatc ggtttaacac
      421 cacattttta tgttgccaaa acgcataaac acagccaaca cccataataa aaactaattg
      481 tgccgcaaca aaattggcat aatgtggctg gtatgaaata gtatggcctg catttatggc
      541 taatgcggtt ataaataaaa cggcaacagg taaactcaaa atacagtaat ttaaatattt
      601 acgaatgctt ttatcagcct tgcgccatgc aacataaatc aggtgtagcg gtattaaaag
      661 cagcaatata atttcgcgca attgcaaacc ggatttttgc aaactattgc ctaatgcagg
      721 taaggcaaat tgaatagaac ctataatgca gtttaagaaa gtagtgggct gaacaaaatc
      781 cgaatttttg cttagttcag taacatagta attattatgc gcatgcatta cggccattcc
      841 ttttaacccg gctacataca tccatagtga aagacctgtt gcagctaacg atacagcaac
      901 aagaaaataa aaaatccgaa tacgtttgcg caagtatatt ataacccaca agccttgggt
      961 aataaaaata taggcagtta gataatggca taaaagtgcg gcaagtgttg caacgatata
     1021 aaacaaaatg tttacaacat ggggttttga attaggttga tagctaaaaa aacaatgaaa
     1081 taaaaagtaa ctgctccaaa tcgtgtaaaa tgttgctgcc gcataaggtc ttgcttcgcg
     1141 cgcatacata attagtaatg gatgaaaagc tgcaacggca gtagcaccaa tggctaaggc
     1201 aggtatttta aatttctttt ttgcaatata gtacactgag aatagcaatg caataccata
     1261 aagcgcagat aacatacgca cagccacatc ggtagctcca aaaagcttac accaaaaatg
     1321 caattgtagt atgtaaaata tgccattgcc accat
//
LOCUS       DAOMAA010000732         1354 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_120539_length_1354_cov_2.267898, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000732 DAOMAA010000000
VERSION     DAOMAA010000732.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120539_length_1354_cov_2.267898"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..1117)
                     /gene="gap"
                     /locus_tag="PLO59_09295"
     CDS             complement(107..1117)
                     /gene="gap"
                     /locus_tag="PLO59_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HQV01341.1"
                     /translation="MSTVKVAINGFGRIGRLVYRQIYDMQGIDVVAINDLTSPAVLAH
                     LLKYDSAQGRFGQEVKATENSIVVNGDDIKIYAQKDPSQIPWGQHDVDVVIECTGFFT
                     DKAKAEAHITAGAKRVVISAPATGDLKTVVFNVNHQILDGKETIISCASCTTNCLAPM
                     AKTLNDSFGIVTGIMSTIHAYTNDQNTLDAPHPKGDLRRARAAAANIVPNSTGAAKAI
                     GLVLPELKGKLDGGAQRVPVITGSVTELYSILNKKVTTEEINAAMKAASNESFGYNED
                     EIVSSDIIGTRFGSLFDATQTKVMQMGDSQMVKTVSWYDNEMSYVSQLVRTVHHFAKL
                     IG"
BASE COUNT          399 a          281 c          226 g          448 t
ORIGIN      
        1 ttgacatgat taaatgattt aaaaaatgaa ccgcaaaaac aatggcagct aagtatcaac
       61 ccggcacctc attgtctttg cggttaaaaa attatttaaa aagttattat ccaatcaact
      121 tagcaaaatg atgtactgtt cgcaccaatt gactaacata actcatctca ttatcgtacc
      181 agctaacggt tttcaccatt tgactgtcgc ccatttgcat aactttggtt tgtgtagcat
      241 caaataaaga accaaacctg gtgcctataa tatcactgct tacaatctca tcttcgttat
      301 aaccaaaact ttcgttgctt gctgctttca tagctgcatt tatttcttcg gtagtaactt
      361 tcttatttaa tatagaatat agttctgtaa ccgatcctgt aattacgggt acacgctgtg
      421 ctccgccatc taattttcct ttaagctcag gtaaaaccaa tccaatggct ttggctgcac
      481 cggtgctatt tggaacaata ttagcggctg cagccctggc tcttcttaaa tcacctttag
      541 gatgaggtgc atccaatgtg ttttgatcgt tagtgtatgc atgaatcgtg ctcataatac
      601 ctgtaacaat tccaaaacta tcgtttaatg ttttagccat aggtgccagg cagtttgttg
      661 tgcaactggc acaactaatg atggtttctt taccatccag aatttggtga ttaacattaa
      721 atacaacggt ttttaaatcg ccggttgcag gagccgatat taccacacgt tttgcgccag
      781 cagtaatatg agcttcggcc ttagccttat cggtaaaaaa accggtacac tcaataacca
      841 catcaacatc atgttgcccc cagggaattt gtgatggatc tttttgtgca taaatcttta
      901 tatcatcgcc attaactact attgaatttt ctgtagcttt tacttcctgg ccaaaacggc
      961 cctgggcact atcgtatttt aataaatgtg ccagcactgc cgggctggtt aaatcattga
     1021 tggctactac atcaatacct tgcatatcgt atatctgacg atacaccagg cggcctatac
     1081 ggccaaatcc attaatagca acttttactg tactcatatc gttaacttaa tttaaaattt
     1141 gagatgcaaa tttacgaata tgcaggcgtt atttaataag aatattttta atttttgatt
     1201 gatgtgtaaa atttgctaaa atcgagtaca gacaacagtt gtacaactct tttatacaag
     1261 caactttaaa tctacctgct ggtattttta aattatttgg cagtaaaacc acagcatgtt
     1321 atctttgccg tcctttaata gaaaatgccg cgtt
//
LOCUS       DAOMAA010000733         1353 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_120713_length_1353_cov_2.473035, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000733 DAOMAA010000000
VERSION     DAOMAA010000733.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120713_length_1353_cov_2.473035"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="PLO59_09300"
     CDS             <1..645
                     /locus_tag="PLO59_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01342.1"
                     /translation="KIKSGTFKVTRTKTVASLPPQVYTSTIEFDASSISTDSVVRHLI
                     SIGDSEQVYYFKDTLYHLYHKSKQCFVKPVDAINFIKAIGTERAFVFMPYVSKSTNRF
                     YNDGNWMPEKLNQMYCSEVLYDSLYSYLRFYLTKRTDNTASGFAIDMKLNASLSQLVS
                     CTYTNIDNYFTNSTTIAVTPLTEGLRQLNAFDISYYLQHYKVDIIMPDSYFKKM"
     gene            854..>1353
                     /locus_tag="PLO59_09305"
     CDS             854..>1353
                     /locus_tag="PLO59_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HQV01343.1"
                     /translation="MKKVLIITYYWPPSGGAGVQRWLKFVKYLRNFGWEPVVYTPSNP
                     ENPVDDASLLKDLPSNLEVLQQPIWEPYDLYKKFIGQKPHQKINAGFISENKKPKLTE
                     KISVFIRGNFFIPDARVFWVKPSVKFLTSYLKENKVDALISTGPPHSMHLIAMQIHKK
                     NNLPWLA"
BASE COUNT          442 a          262 c          230 g          419 t
ORIGIN      
        1 aaaataaaat cagggacatt taaagttacc cgaaccaaaa cggtagcttc cttaccacca
       61 caagtttata cctcaactat tgaatttgat gcgtcttcta tttctaccga ttctgtagtt
      121 aggcatttaa tttctattgg cgattcggag caggtctatt attttaaaga tacgctttat
      181 catttgtatc acaagtccaa acaatgtttt gtaaaacctg ttgatgcaat aaattttata
      241 aaagctattg gaaccgaaag agcatttgtt tttatgccct acgtttcaaa aagtaccaat
      301 cggttttata acgatggaaa ctggatgccc gaaaagctaa accagatgta ttgtagtgaa
      361 gtattgtatg attcgttata cagttattta aggttttact taacgaagcg cactgataat
      421 actgcaagcg gatttgcaat tgacatgaaa ctaaatgctt ccttatctca attagtaagt
      481 tgcacctata ccaacattga taattatttt acaaactcta ccactattgc cgtaactcct
      541 ttaacagaag gattgcgcca attaaacgcc tttgatattt cgtattactt gcagcattac
      601 aaagtagata tcattatgcc cgatagctac tttaagaaaa tgtaaaggca tattaaagcg
      661 aatcaaagca aaactgcctc acatagctat gtaaccccca tcaccaattt tgtgatagtt
      721 taagtatctt ctaaaattct aaaaagaaaa acctctcaag aaattgggag gtttttttat
      781 tggcatatat tcgccaccac gaaaacaaac agacaggctt cttcatcaac gaagcatcga
      841 aacaagtttc agtttgaaaa aagttctaat cattacatat tattggccac caagtggagg
      901 tgccggtgtg cagcgttggc ttaagtttgt aaagtacctg cgcaactttg gttgggagcc
      961 tgttgtgtac acgccttcca atcccgaaaa tcctgttgat gatgcttcgt tgttaaaaga
     1021 tttgccttca aatcttgaag tgctgcagca acccatttgg gagccttacg atttgtataa
     1081 aaagtttatc ggccaaaaac ctcatcaaaa aatcaatgcc ggttttattt ctgaaaacaa
     1141 aaaaccaaaa ctgacagaaa aaatttctgt gtttatacgt ggcaattttt ttattcccga
     1201 tgcacgcgtt ttttgggtaa agccttcggt aaaattctta acatcttatc tgaaagaaaa
     1261 taaggtggat gccctgattt ctacaggtcc gccacatagc atgcatttaa tcgccatgca
     1321 aatccacaaa aaaaacaact tgccttggtt ggc
//
LOCUS       DAOMAA010000734         1353 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_120715_length_1353_cov_2.472265, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000734 DAOMAA010000000
VERSION     DAOMAA010000734.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120715_length_1353_cov_2.472265"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1353
                     /locus_tag="PLO59_09310"
     CDS             <1..>1353
                     /locus_tag="PLO59_09310"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HQV01344.1"
                     /translation="AVPGGGTDYKMEFIPDQVSQAVLITQIQALQAQGQKVIISIGGA
                     TAPITLSTDAERDTFIVRMNGIINTYGFNGIDIDLEGSSLSLTGGTIANPTTPAITRM
                     IFAIKQIMANYHQAKGKKLLLTMAPETAFVQGGMSAYTGIWGAYLPVIHALRDSLDML
                     NVQLYNSGSMYGIDGNIYTQGTADFIVAMTEAVIKGFNTAGGFFQGLPASKVGIGLPA
                     CINAAGGGFTDTATVRAAANYLLGEGPKPGSYTLQTPGGYPALKGMMTWSINWDKVAT
                     CGGVYEFASNFEKIFQPDACTTCPAPTNTTATNITATAAKLNWQVNNCAVAYQVKYKV
                     NGTTNWITANVNNNTGFINVTGLQPNTKYRWKVRSKCATNPNVFSDFSTIKTFTTALL
                     KSGLNSLAEIKVYPNPASDFINIETGTETLPISLLIFNHSGQKVLESTITQNQQIDIR
                     "
BASE COUNT          439 a          270 c          271 g          373 t
ORIGIN      
        1 ttgctgtgcc cggtggtggt accgattata aaatggaatt tattcccgat caggtttcac
       61 aagctgtatt aataacacaa atacaagcat tgcaggcaca aggacaaaaa gtaattataa
      121 gcattggcgg tgcaacagca cccattacat taagcaccga tgccgagcgc gatacattta
      181 ttgtgcgtat gaatggcatt attaacacgt atggttttaa tggtattgat attgatttag
      241 aaggcagttc attatcgctt accggtggca caattgctaa tcccaccacg ccagccatta
      301 cccgaatgat atttgccatt aaacaaatta tggccaacta ccatcaagct aaaggcaaaa
      361 aactattgtt aaccatggca cccgaaacgg cttttgtgca aggaggcatg tcggcataca
      421 cgggtatttg gggtgcttac ttacccgtaa tacatgcctt gcgcgactca cttgatatgc
      481 taaacgtaca actttataac agtggttcta tgtatggtat tgatggtaac atttacacac
      541 aaggtactgc cgattttatt gtggctatga cagaagcagt tataaaaggt tttaatactg
      601 ccggtggatt ttttcaggga ttgcctgcat caaaagttgg cattggacta ccggcctgca
      661 taaatgcagc aggtggtggt tttacggata cggctaccgt tagggctgca gcaaattatt
      721 tacttggcga aggcccaaaa cccggcagtt atactttgca aactcccggt ggctatcctg
      781 ctttaaaagg gatgatgacc tggagtataa actgggataa ggttgctaca tgcggtggtg
      841 tgtatgagtt tgcaagcaat ttcgaaaaaa tatttcagcc cgatgcctgc accacatgcc
      901 ctgctccaac aaatactaca gcaacaaata tcacagctac tgcggctaaa ttaaactggc
      961 aggttaataa ctgcgctgtt gcttaccagg taaaatataa agtaaatggt acaacaaatt
     1021 ggataacagc taatgtaaac aacaatacag gttttattaa tgtaacaggt ttacaaccta
     1081 acaccaaata ccgttggaaa gtaagaagca aatgtgcaac taaccccaat gtgttttctg
     1141 attttagcac tataaaaaca tttacaacag ccttgttaaa aagcggttta aattcgttgg
     1201 ctgaaataaa agtttatccc aatcctgcat cagacttcat aaacatagaa acaggtactg
     1261 aaacattacc aatatcattg ctgattttta atcattcggg ccaaaaagtt ttggaatcca
     1321 ccataactca aaatcaacaa attgatataa gaa
//
LOCUS       DAOMAA010000735         1351 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_120996_length_1351_cov_3.817901, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000735 DAOMAA010000000
VERSION     DAOMAA010000735.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120996_length_1351_cov_3.817901"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..572
                     /locus_tag="PLO59_09315"
     CDS             3..572
                     /locus_tag="PLO59_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HQV01345.1"
                     /translation="MSKHKIVVAITGASGSMYASLLLQKLQQLNKQIEEVGLVMSSNS
                     CDVWQYEMGNTDYKNVPFKNYASNDFFAPFASGSANFNTMIICPCSMGTLARIAAGIS
                     NDLITRAADVILKERRKLILVTRDTPLSLIHINNMKAVTEAGAIVCPASPSFYAKPQT
                     IEDVAQSVVNRVIDLAGLQHDTFRWASKN"
     gene            673..861
                     /locus_tag="PLO59_09320"
     CDS             673..861
                     /locus_tag="PLO59_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015329747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01346.1"
                     /translation="MFEYTKQILQKVSFDKKLFRKELVKALKWLKPDEKKMLMVWALA
                     NFGNKYTDIISQVFKKMA"
     gene            complement(845..>1351)
                     /gene="queG"
                     /locus_tag="PLO59_09325"
     CDS             complement(845..>1351)
                     /gene="queG"
                     /locus_tag="PLO59_09325"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015807712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HQV01347.1"
                     /translation="KRSGLGWIGKNANLINPKQGSFFFLAELIIDLKIEPDVRIQNYC
                     GTCTRCIDSCPTQAIIKPSVIDGSKCISYFTIELKDAIPYSYKNQFENWAFGCDVCQD
                     VCPWNRFSKPHNETALQATETLLEMSANDWQEITEDVFKKLFKNSAVKRTGFKGLIRN
                     LAFIKPSS"
BASE COUNT          430 a          244 c          260 g          417 t
ORIGIN      
        1 agatgagcaa gcataaaatt gtggtggcta taaccggtgc aagtggcagt atgtatgctt
       61 cgcttttatt acaaaagttg cagcagctaa acaaacaaat tgaagaagtg ggtttggtaa
      121 tgtcgtcaaa ttcttgcgat gtttggcaat atgaaatggg taatacggat tataaaaacg
      181 tgccttttaa aaactatgca tctaacgatt tttttgcccc tttcgcaagt ggttcagcta
      241 acttcaatac aatgattata tgcccttgca gtatgggtac tttggcaagg attgccgcag
      301 gtataagtaa cgatttaatt acacgcgctg ctgatgtaat tttgaaagag cgcagaaaat
      361 tgattttagt tacgcgcgat acaccactta gcctcataca tataaataat atgaaagctg
      421 taaccgaagc aggcgctatt gtttgcccgg cttcgccatc gttttatgct aagcctcaaa
      481 ccattgaaga tgttgcccaa agtgttgtaa acagggtaat agacttagca ggtttgcaac
      541 atgatacgtt tcgctgggca tcaaaaaact aataaacaaa ccaattgtaa aatagtttat
      601 taaactacaa tttgtacttg cataaatcgt ttttactttc aaacttagtc ttacttttta
      661 aacacctttt ttatgtttga atacaccaag cagattttac agaaggttag tttcgataaa
      721 aaacttttcc gtaaagaatt ggtaaaggcc ctaaagtggc taaagccaga cgaaaagaaa
      781 atgctaatgg tatgggcatt ggctaatttt ggcaacaaat acacagatat tatttcgcag
      841 gtttttaaga agatggcttg ataaaagcca ggttgcgtat taaaccttta aatccggtac
      901 gttttacagc actgttctta aatagctttt taaatacatc ttcagtaatc tcctgccagt
      961 cattagcgct catttcaagc aatgtttccg tagcctgcaa tgcagtttca ttatgtggtt
     1021 tactaaagcg gttccaaggg caaacatcct gacatacatc gcagccaaaa gcccagtttt
     1081 caaattggtt tttataactg taaggaattg cgtctttaag ctcaatggta aagtatgaaa
     1141 tacacttact tccatcaata acactgggtt taataatggc ttgagtaggg caggaatcaa
     1201 tgcatcgtgt acatgtgcca cagtagttct ggatacgcac atcaggctca atttttaaat
     1261 caataattag ttcggctaaa aagaaaaacg aaccttgctt ggggttaata aggttggcat
     1321 tttttccaat ccaacccaaa ccgcttcgtt t
//
LOCUS       DAOMAA010000736         1350 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_121226_length_1350_cov_2.613127, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000736 DAOMAA010000000
VERSION     DAOMAA010000736.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121226_length_1350_cov_2.613127"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1350
                     /locus_tag="PLO59_09330"
     CDS             <1..>1350
                     /locus_tag="PLO59_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01348.1"
                     /translation="FTGFKPPYAGQTLIPATSPFAAFVINNHYPSYLNLGRLTNSYPL
                     TIRNHASGIIANHANVSIVNTTFQNISQVGAYGSFPATPSGFAIYARASNNRNIIYSG
                     QGNNAADPLSFDNCRGAVSIQSTSATIENAKMNVIRMGVFNRLSQAPSSIKIYNNTID
                     VIGFRNLAGIASIFSGTSKFDVIANTIHVQASSFLQRSSCIRLDDNPSADNSLMHVTG
                     NVCRILNQTTYGILMQNIARLSLNDNFVFCNQGTFTGSQYHYAFSADNCAEVTFYNNT
                     VNSANSVFQPNGMGFYNYISPRWIYNCNKAYQLHTGFRFDEDCSSPQNFMGNEIEANN
                     YGLYINFPSGAIGIQTHRGNIWSLNNSLFASPHGAAGVYNGANPPQSEFTVHTLTAPA
                     NVYNPVLKSTNLINFQSGTPAQCILISPTPPADEIALAEQAVAQADSSTENENGTLG"
BASE COUNT          400 a          299 c          255 g          396 t
ORIGIN      
        1 attttacagg ttttaagcct ccctatgcag gccaaacatt aatacctgca acctcgcctt
       61 ttgccgcatt tgttataaac aaccattacc cttcttattt aaatttaggc cgattgacaa
      121 attcctatcc cttgactatc agaaatcatg cttcgggtat tattgccaac catgccaatg
      181 taagtattgt aaatactact tttcaaaata taagtcaggt aggtgcttat ggcagttttc
      241 cggctacgcc cagtggcttt gcaatttatg cccgtgcatc taataacaga aacattattt
      301 atagtggaca aggcaataat gcagccgacc ctctttcatt tgataattgt aggggagctg
      361 tttccattca aagtacctcg gcaactattg aaaatgcaaa aatgaatgta atacgaatgg
      421 gcgtttttaa tcgcctatcg caagcaccat cgagcattaa aatttataat aataccattg
      481 atgtaattgg ctttaggaac ttggccggta ttgccagcat tttttcgggt accagtaaat
      541 ttgatgtaat tgccaatacc atccatgtgc aagcctccag ttttttgcag cgctccagtt
      601 gcatccgttt agatgataac cccagcgctg ataattcact gatgcatgta accggcaatg
      661 tttgccgtat tttaaaccaa accacttatg gcattttaat gcaaaatata gcccggttaa
      721 gcttgaacga taattttgtg ttttgtaacc agggtacatt tacaggcagc caataccatt
      781 atgcattcag tgccgataac tgcgctgagg ttacttttta caacaatacc gttaacagtg
      841 ccaattctgt ttttcagccc aatggtatgg gcttttacaa ttacataagc ccgcggtgga
      901 tttataattg caataaagcg tatcagctac acaccggttt caggtttgac gaagactgca
      961 gtagccccca aaactttatg ggcaatgaaa tagaagccaa taattatggc ttatacatta
     1021 atttcccttc aggtgccata ggcatacaaa cacaccgtgg caatatttgg agtttaaata
     1081 acagcttatt tgcaagccca cacggtgcgg caggggtgta taatggtgcc aatccaccac
     1141 aatctgaatt tacagtgcat acccttactg cccctgctaa tgtgtacaat ccggtactga
     1201 aatcaacaaa cttgataaat ttccagagtg gcactccggc tcaatgtatt ttaataagtc
     1261 caacaccgcc tgccgatgaa atagcgttgg ccgagcaagc agttgcccaa gccgatagca
     1321 gtactgaaaa tgaaaatgga accttaggcc
//
LOCUS       DAOMAA010000737         1348 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_121535_length_1348_cov_3.865429, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000737 DAOMAA010000000
VERSION     DAOMAA010000737.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121535_length_1348_cov_3.865429"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            378..767
                     /locus_tag="PLO59_09335"
     CDS             378..767
                     /locus_tag="PLO59_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014034194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HQV01349.1"
                     /translation="MDANILAYNTNTLTHANICNLLADTISGTLTEANGKIWHAHPVW
                     FINDNPIVGYSEQKKGIRLMFWSGAGFNDAKLNVRGEKYKDASVFYNNLNEVDTAEII
                     GWLKQSRVVQWDYKNIVKRKGKLERLF"
     gene            848..>1348
                     /locus_tag="PLO59_09340"
     CDS             848..>1348
                     /locus_tag="PLO59_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01350.1"
                     /translation="MLNGIKITYHNALLIMGAFLLNITMLYAQKRFDVFYVAGNYNFL
                     KSTNINQNNYEKAWAANLSIPFLFNDSSIWLTTLDYQHYNLPNEHNTADNPVTVFNLH
                     GCIIRTGYIKKISSKQAIHALFIPRVMTDFNATLGNALQLGGMVMYEKIKNKNLMWRA
                     GVIYNQE"
BASE COUNT          450 a          198 c          251 g          449 t
ORIGIN      
        1 atgccgtcat ggtgttttga ttgttttttt gagcaaagcc ttgcaaagca gttacggtta
       61 gcaatagtat taaaacacgt ttcataagcg aataatttta ggtaacagac gtaaaaatag
      121 aagttttgtt atggcaatat ggcggttaac ttttgtttaa ccgctggcaa tgtattggct
      181 ttacgcaaat atttatcagg tattcaactt gtaattacat ttttaatttt catgctattg
      241 tgtcaaaact attttactac gtttataaac ctgctttggt gttagcgttg cttaggctgc
      301 gaacaatggc tttatttgtt tgctgtaaaa attaactgct tacatttagc ttttgattaa
      361 ttataaatcg ttttacaatg gatgcaaata ttttagcata caataccaat acacttaccc
      421 atgcaaacat ctgcaatttg ttggccgaca ccatttcggg aacgttaacc gaggctaatg
      481 gaaaaatttg gcatgcacat ccagtatggt ttataaatga taaccccatt gtaggttata
      541 gcgaacagaa aaaaggcatc cggcttatgt tttggagtgg tgccggtttt aacgatgcta
      601 aactgaatgt gagaggcgaa aaatataaag atgcctcggt gttttataat aatctgaatg
      661 aagttgatac agcggaaatt attggctggt taaaacaatc gagggttgta caatgggatt
      721 ataaaaacat tgtaaaacga aaaggaaaat tagaaaggtt gttttaacaa ctattgttgc
      781 agataaaatg tataaccatt aaatcagatt ttctgttaat ttaatgtatg gttttaagta
      841 caattttatg ttgaacggaa taaaaatcac ataccataat gcactactaa ttatgggcgc
      901 ttttttgttg aacattacca tgttgtatgc acaaaaacga tttgatgttt tttatgtagc
      961 gggtaattat aattttttaa aatcaacaaa tattaatcaa aacaattacg aaaaagcatg
     1021 ggcagctaat ttgagtattc cgtttttatt taatgattcg tcaatatggt taactacttt
     1081 agactatcag cattacaacc tgcccaatga gcataataca gcagataatc cggtaaccgt
     1141 ttttaattta catggctgca tcatacgtac cggctacatt aaaaaaataa gcagcaagca
     1201 agctatacat gcgcttttta tacctcgggt aatgactgat tttaatgcaa cattgggcaa
     1261 tgctttgcag ttaggcggta tggttatgta tgaaaaaata aaaaacaaaa accttatgtg
     1321 gcgcgctggt gttatatata accaagag
//
LOCUS       DAOMAA010000738         1347 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_121756_length_1347_cov_2.828173, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000738 DAOMAA010000000
VERSION     DAOMAA010000738.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121756_length_1347_cov_2.828173"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1282)
                     /locus_tag="PLO59_09345"
     CDS             complement(<1..1282)
                     /locus_tag="PLO59_09345"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HQV01351.1"
                     /translation="MKFFLHFRLLLLLFVFNATIGFGQRYNFTNYHISDGLPINHILC
                     VEQVRNGQLWIGTTHGLSIFDGNNFAKLNNELALNTNPIKCISQDNMGNVWIGTANNG
                     LLCYRGNKRKQININGNNFNSVNAIAADSTGYLWICTNNGIYTIKDQTNITKLNIKNL
                     PSDTIHKIICDAKGNVWIATSKGLVVCRKKQPDQAIKIYGLGPGVVSAMCLDNQNNIW
                     IGAQNDLYKFDGQLTKVHLINPLSNAKITSITQDINGQIWLATNNEGIGKLVKNYIKM
                     YAVKHGLANNRIQQIKSDREGNLWMATLNGLSKFTGDRFIAYTTNEGLSNNDVLSLFV
                     ADSNHVWVGTNAGGLNLLSNNSFTKVSNGFISPVTTVFKIIRDANSNYWFATSNGLVK
                     YSEATKKYSKPIDKLANKVIYTILEGSNGKMYFGT"
BASE COUNT          402 a          245 c          221 g          479 t
ORIGIN      
        1 cggtaccaaa atacattttg ccattactgc cttctaatat tgtatagata actttgttgg
       61 caagcttgtc gatgggtttg ctgtattttt tagttgcttc gctatactta acaagtccgt
      121 ttgatgttgc aaaccagtag ttggagtttg catctctgat aattttaaaa acagtggtta
      181 ccggactaat aaagccattg ctgacttttg taaaactatt gttgcttaat aaatttaaac
      241 cacctgcatt agtgccaacc catacatgat tactgtcggc aacaaaaaga cttagcacat
      301 cattattact taagccttcg ttggttgtgt aagcaataaa tctatcgcct gtaaatttac
      361 taagaccatt taaagttgcc atccacaaat taccttcacg gtcagatttt atttgttgta
      421 ttctgttgtt ggcaaggcca tgttttacag catacatttt aatgtaattt tttacgagtt
      481 tgcctattcc ttcgttattg gttgcaagcc atatttgacc gtttatatcc tgcgttattg
      541 atgtaatttt tgcattactt aatggattaa ttaaatgaac ttttgtaagc tgtccatcaa
      601 acttatacaa atcgttttgt gcgcctatcc aaatattgtt ttggttatct aagcacattg
      661 cgcttaccac accggggcct aagccataaa ttttaattgc ctgatcgggt tgttttttac
      721 gacaaacaac caatcctttt gaggtagcta tccaaacatt gccctttgca tcacaaatga
      781 ttttatgaat agtatcagaa ggtaaatttt taatatttag tttggtgatg tttgtttggt
      841 ccttgattgt gtagatacca ttgtttgtac aaatccataa atagcccgtg ctatccgcag
      901 caattgcatt aacactattg aagttgttgc cattgatatt aatttgttta cgcttattac
      961 cacggtaaca taacaagcca ttgtttgcag taccaatcca tacattaccc atattatcct
     1021 ggctaataca ttttattgga tttgtgttta aagcgagctc attattaagt ttagcaaagt
     1081 tgtttccatc aaaaatactt agcccgtggg tggtgcctat ccacagttga ccgttacgta
     1141 cttgctcaac acaaagtata tggttaatag gcaaaccatc agaaatatga taattggtga
     1201 aattgtatcg ttggccaaaa cctattgttg cattaaaaac aaataaaagt agcagcagcc
     1261 taaagtgtaa gaaaaatttc acgtaactca acgaagtaca aataatgccg ttgcaaaata
     1321 catttaattt ttattttcca aactcat
//
LOCUS       DAOMAA010000739         1346 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_121955_length_1346_cov_2.508133, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000739 DAOMAA010000000
VERSION     DAOMAA010000739.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121955_length_1346_cov_2.508133"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1346)
                     /locus_tag="PLO59_09350"
     CDS             complement(<1..>1346)
                     /locus_tag="PLO59_09350"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010600684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HQV01352.1"
                     /translation="FPEDYHADVQVMITLISADKDIMPDALAGLAASAALTLSNIPFN
                     GPISEVRVARIDGNMVINPTIKDLERADIDLIVAGSADNIVMVEGEMSEVSEADMVTA
                     IKTAHDAIKNQCQAQLDLLAKCGGVEKQTYSHETHNDDLRKKVSDATYQKVYEIALAG
                     NPNKTLRAEAFKNVLNEFVTTLTEDELAQMELVKKYYHDVEYDAVRNAILNEGKRLDK
                     RQTTEIRPIWCEVDYIPSAHGSSIFTRGETQSLTTVTLGGKMEEQIIDGVMFEGMNKF
                     LLHYNFPGFSTGEVRPNRGTGRREVGHGNLAMRSIKKVLPPESENPYTIRVVSDILES
                     NGSSSMATVCAGSLALMDAGVKLRSTVAGIAMGLISDGKGKFAVLSDILGDEDHLGDM
                     DFKVTGTKNGIVACQMDLKVQGLPYEVLAQALEQARLGRLHIIGEMEKVKSVANDD"
BASE COUNT          350 a          316 c          241 g          439 t
ORIGIN      
        1 gtcgtcattg gcaactgatt ttactttttc catttcgcca ataatatgta aacggcctaa
       61 acgcgcttgc tccaaagcct gtgctaaaac ttcataaggc aagccttgca cttttaagtc
      121 catctggcat gcaacaatac cgttctttgt tccggtaacc ttaaagtcca tatcgcccaa
      181 gtgatcttca tcacctaaaa tgtcgcttag tacagcaaat tttcctttgc catcgctaat
      241 taaacccata gcaataccgg caacggtgct gcgtaatttt actcctgcat ccattaatgc
      301 cagcgatcct gcacaaaccg tagccatact tgacgagcca ttcgactcta aaatatcgct
      361 cactactctt atggtgtatg gattttctga ctcgggtggt aatacttttt taatactgcg
      421 catggctaag ttgccatggc ctacttctct gcgacctgta ccacggtttg gcctaacctc
      481 accggtcgaa aaacccggga aattataatg caacaagaac ttattcatcc cttcgaacat
      541 tacaccgtca ataatttgtt cttccatttt accccctaag gttacggtgg ttaacgattg
      601 tgtttcgcca cgtgtaaata tagaactgcc atgtgccgat ggtatataat ctacctcaca
      661 ccaaataggt cgtatttctg tagtttggcg tttatctaag cgtttgcctt catttaatat
      721 cgcattacga accgcatcat attcaacatc gtggtaatat ttttttacca gctccatctg
      781 tgctaattca tcttcggtaa gtgtggtaac aaattcgttc aaaacatttt taaatgcttc
      841 ggcacgtaag gttttgtttg ggttaccggc caaggcaatt tcatatacct tttgataggt
      901 tgcatcgctt acttttttac gcaaatcatc gttatgtgtt tcatggctgt aggtttgttt
      961 ttcaacacca ccacatttgg ctaacaaatc taattgggcc tggcattgat ttttaatggc
     1021 atcatgggca gttttaatgg ctgttaccat atctgcttcg cttacttcgc tcatctcgcc
     1081 ttcaaccatc acaatattat cagcactgcc tgcaacaatt aaatcaatat cggcacgctc
     1141 taaatcttta atggttgggt ttattaccat gttgccatca atacgtgcta cgcgtacttc
     1201 tgaaataggc ccgttaaatg gtatgttact taatgttaga gctgctgatg cagccaaacc
     1261 ggctaaggca tcgggcataa tatctttatc ggctgatatg agggtaatca ttacctgtac
     1321 atcggcatga taatcttccg gaaata
//
LOCUS       DAOMAA010000740         1340 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_122943_length_1340_cov_3.185992, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000740 DAOMAA010000000
VERSION     DAOMAA010000740.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122943_length_1340_cov_3.185992"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..1128)
                     /locus_tag="PLO59_09355"
     CDS             complement(64..1128)
                     /locus_tag="PLO59_09355"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HQV01353.1"
                     /translation="MVHTKTTPPTFICGSNAFTDFAQLLNENFDSYLKILIVDVNTHR
                     YCLPVLKQNIKGLAKAPIIKLPATEYAKSMESVSVIINKLIKLKAAKKTVIINLGGGV
                     ITDLGGFCASIYQRGIPCIQVPTSLVAMTDAAIGGKTGINFGQLKNYIGTFTHANATI
                     IYPDFLKTLPPQQYLSGMAEVFKHGLIGDKKIYNQLLSTSWLQPISNQLLLQSIAVKQ
                     KIVHADFYEKNKRKLLNAGHTVGHAIESYSHTTGKPLLHGYCIAIGLIVECYIGHVLG
                     LLSLKHLVEITHCIHQNFSFDPFTVKEVNQIIALMYFDKKNNNHQVNFSLVEKPGKAA
                     VDCYASADVIKAGFMFFNAS"
BASE COUNT          408 a          237 c          244 g          451 t
ORIGIN      
        1 caatgtaacc aaccgtttaa tgtacgtaca tacatttagc aaatttttgc atttattaca
       61 aaactaagac gcattaaaaa acataaatcc tgctttaatt acatcggccg atgcataaca
      121 atcaacagca gctttaccgg gcttttctac cagactaaaa tttacctgat ggttgttgtt
      181 ttttttgtcg aagtacatca aagcaattat ttgattaact tctttaacgg taaatggatc
      241 gaagctaaaa ttttgatgga tgcaatgtgt aatttccact aagtgcttaa gcgataataa
      301 accaagtaca tggcctatat aacattcaac aattaagcct atggctatgc aatagccatg
      361 tagtaggggc ttgccggttg tatggctgta gctttcgata gcatggccta cggtatggcc
      421 tgcattaagc aatttgcgtt tgtttttttc gtaaaaatcg gcatgtacaa ttttttgttt
      481 aacagcaatt gattgcagca gtaactgatt tgaaatgggt tgtaaccagg acgttgaaag
      541 cagttgattg taaatttttt tatcgccaat aagaccatgt ttaaaaacct cggccatgcc
      601 acttaagtat tgctgtggtg gtaatgtttt taaaaaatca gggtaaatta tggtggcatt
      661 agcatgggta aaggttccga tatagttttt tagttgacca aagttaattc cggttttgcc
      721 tccaatggct gcatcggtca tagcaactaa actggtaggt acttgaatgc aaggtatgcc
      781 acgttgataa atggatgcac aaaaaccacc taaatcggtt ataacaccac cacccaagtt
      841 tataataact gttttttttg ctgccttaag cttaataagt ttgtttatta ttaccgacac
      901 actttccatg cttttagcat attcggtagc cggcaattta attattggag cctttgccaa
      961 gccttttata ttttgtttta aaacaggtaa acaatacctg tgggtattaa catcaacaat
     1021 taaaattttt aagtagctgt caaagttttc gttaagcagt tgcgcaaaat cggtaaatgc
     1081 attactgcca caaataaatg ttgggggtgt tgtttttgta tgaaccatta aatttaaatt
     1141 tcaatgttgg ccaagttagg ttattcggcc acatggttaa gcaaaatggc attagcagca
     1201 ttaaaaaatt atctttgcac gccccaactt tagatttttc ttttatgagc caccaatcgc
     1261 aaaccgtttc attcgacaat atcgaaactg cattttcggg caaaaccatg gccgatttaa
     1321 accgctctta ttttttgttt
//
LOCUS       DAOMAA010000741         1339 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_123168_length_1339_cov_2.593458, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000741 DAOMAA010000000
VERSION     DAOMAA010000741.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123168_length_1339_cov_2.593458"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..594)
                     /gene="dut"
                     /locus_tag="PLO59_09360"
     CDS             complement(163..594)
                     /gene="dut"
                     /locus_tag="PLO59_09360"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012472298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HQV01354.1"
                     /translation="MNVIINNTGKHALPAYETAGAAGLDLRANINQAIELLPMQRALI
                     PTGLFMQLPIGTEGQIRPRSGLAYKHGITVLNAPGTIDADYRGEVMVLLINLSDKTFT
                     INDGDRIAQLVVAPHHTIQWQTNQTLNQTNRGTGGFGHTGQ"
     gene            complement(665..>1339)
                     /locus_tag="PLO59_09365"
     CDS             complement(665..>1339)
                     /locus_tag="PLO59_09365"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HQV01355.1"
                     /translation="IYGAVYKLAMLMTMFVQAFRFAAEPYFFARQKDADVKQVYAYVV
                     RYFVLGSALVFLIVMLYIDVFKYFIGSDSKNEYWEGLHVVPVLLLANLCLGVYLNISM
                     WYKWSGQTKYGAWFSVGGALITLALNFLLIPKYGYTGCAYTTLICYGSMLLVSYIIGA
                     KKYPVYYDLKSMLTYVIAAIALYACSLWFDNYAQPGLPLLLTFNSMLLSIYVAAIWYS
                     ERSKKI"
BASE COUNT          404 a          256 c          278 g          401 t
ORIGIN      
        1 acttttgcct gccaccggta ttaatggttt tggtactgta agtgtgtgtg ggcgcattct
       61 tttgcccata cctgccatag gaacaataat tttcatgtga tattttgttt tcgtttctgc
      121 tggatataat ttgtattgat tgatacgcgg tgttgcaatt tgttactgac cggtatgtcc
      181 aaaaccaccg gtacccctgt tggtttggtt aagcgtttga ttggtttgcc actgtatggt
      241 atgatgcggg gcaactacta attgcgctat gcgatcgcca tcattgatgg taaatgtttt
      301 gtctgataaa ttgatgagta acaccattac ttcgccacga taatcggcat caatagtgcc
      361 cggggcatta agtacggtaa tgccatgttt ataagccaaa ccgctgcgag ggcgtatttg
      421 gccttcggta ccaataggta gttgcataaa taaaccggta gggattaatg cccgctgcat
      481 gggtaacagt tcaatagcct gatttatatt ggcacgcaag tcgaggccgg cagcaccggc
      541 agtttcatat gcaggtaatg catgtttgcc ggtattatta ataattacat tcatctgatt
      601 gctaacaaaa tggggcttcg aattgattta aataaaactg agctttttaa aagggcgtgc
      661 gaatttagat ttttttactt cgttcgctat accatattgc cgccacatag atggataaaa
      721 gcattgaatt aaatgttaaa agtaggggca aaccgggttg ggcataatta tcaaaccata
      781 aactgcaggc gtaaagcgct attgcggcta ttacataggt tagcatgctt tttaagtcat
      841 aatatacagg gtattttttt gcgcctatta tgtatgaaac cagcaacata ctgccataac
      901 aaattaaggt ggtgtaggcg cagccggtat aaccgtattt tggaataagt aaaaaattta
      961 atgccagggt aattagggca ccaccaacgc taaaccatgc accatatttg gtttggccac
     1021 tccatttata ccacatgctt atatttaaat aaacacccaa acataaattg gcaagtagta
     1081 aaaccggcac tacatgtagg ccttcccagt attcgttttt actatcagag ccaataaagt
     1141 atttaaacac atcaatgtaa agcattacaa ttaaaaatac caaagcactg cctaaaacaa
     1201 agtaacgaac aacataagca tacacttgtt ttacatcagc atctttttga cgggcgaaga
     1261 aatagggttc ggctgcaaac ctgaaagctt gtacaaacat ggtcattaac atggccaatt
     1321 tgtaaacggc accataaat
//
LOCUS       DAOMAA010000742         1338 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_123336_length_1338_cov_2.962588, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000742 DAOMAA010000000
VERSION     DAOMAA010000742.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123336_length_1338_cov_2.962588"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..1199)
                     /gene="wecB"
                     /locus_tag="PLO59_09370"
     CDS             complement(60..1199)
                     /gene="wecB"
                     /locus_tag="PLO59_09370"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HQV01356.1"
                     /translation="MIKIVTVIGARPQIIKAAALSRAIKNSFADKIAEVIVHTGQHYD
                     NNMSQVFFDELRIPASNYNLQVGSGSHGIQTAKMIEGIEQILVAEKPHALLVYGDTNS
                     TLAGAIAASKLHIPIVHVEAGLRSFNKKMPEEINRILCDHCATLLFSPTVAGYNNLIN
                     EGFNKNALPPYSNDNAFIVHCGDVMYDNSLFFEAEANRKSTVLNKINKPYVLVTVHRA
                     ENTDDADALNNLFQAIQNLSLAYQVSFVLPLHPRTAHLLPKSLTPELYQSIVDNKLIQ
                     IIEPVSFLDMIALESNAKLIVTDSGGVQKEAYFFKKPCIILRQQTEWTELVACGSAIL
                     AGANIARIEDAFAYYYSNDISLQFPPIFGDGHAAEFICKTIIEKL"
BASE COUNT          405 a          246 c          252 g          435 t
ORIGIN      
        1 tcaggactta cacttgcatc aaacaaaccg gctatgccac acatataaaa gttatttgat
       61 taaagttttt cgataatggt tttacaaata aattccgctg catggccatc gccaaaaatg
      121 ggcggaaact gaagcgaaat atcattacta taataatatg cgaatgcatc ttcgatacgc
      181 gcaatgtttg caccggctaa aatagctgaa ccacatgcaa ccaactcagt ccattcggtt
      241 tgttggcgca atatgatgca aggtttttta aagaaatagg cttctttttg tacgcctcca
      301 ctatcggtaa caatcagttt tgcattgctt tcgagcgcta tcatatctaa aaaagaaacc
      361 ggttcgatta tttgtatcaa tttattgtca actatgcttt gatacaattc gggcgtgagt
      421 gattttggta acaagtgtgc tgtacgtgga tgtaatggca atacaaatga aacttgatac
      481 gcaagcgata aattttgaat ggcctgaaat aaattattta gtgcatcggc atcatcggta
      541 ttctctgctc tgtgtacagt aaccaataca tacggcttat taattttatt taatacggtt
      601 gatttacgat ttgcttctgc ttcaaagaat aagctgttgt cgtacatcac atcgccacaa
      661 tgcacaataa atgcattgtc gttactataa ggtggtaaag catttttatt aaagccttcg
      721 ttaattaaat tattgtaacc ggccacagtt ggcgaaaaca ataaggtagc gcaatgatcg
      781 cataaaatgc ggttaatttc ttcgggcatt tttttattaa acgaccttag gccggcttca
      841 acatgtacga tgggtatatg cagttttgat gcagcaatgg caccggctaa tgttgaattg
      901 gtatcgccat aaaccaataa tgcatgtggc ttttcggcta ctaaaatttg ttcaatacct
      961 tcaatcattt ttgcggtttg aataccatgt gaacctgaac ctacctgcaa attgtaatta
     1021 ctggcaggta tgcgtaactc atcaaaaaac acttgcgaca tattgttgtc gtaatgctga
     1081 ccggtgtgca caatgacttc ggcaatctta tcggcaaaac tattttttat ggcgcgactt
     1141 aaagcagcgg ctttaataat ttgtgggcga gcacctataa cagttacaat ctttatcatt
     1201 tctttaagag gtcatttgat ttaaaacttc aaccaattgt tgcgttaggt ttttacgaga
     1261 ataacggtca acgctttgtg aagtaacttt aagtttattg tttttaaact gttcataaaa
     1321 agcacttaca gtagcttt
//
LOCUS       DAOMAA010000743         1337 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_123558_length_1337_cov_2.376755, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000743 DAOMAA010000000
VERSION     DAOMAA010000743.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123558_length_1337_cov_2.376755"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="PLO59_09375"
     CDS             complement(<1..563)
                     /locus_tag="PLO59_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019948231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HQV01357.1"
                     /translation="MNQTEIINDSNTTSEKITNHKILIYQVMTRLLGNKQTNLIPYGT
                     IAQNGVGKFNDINDAALMSLKGMGFTHLWYTGIIEHATLTDYSAFGIVADDPDVVKGR
                     AGSAYSIKDYYDVNPDLAVDVKNRMAEFESLVMRTHKNNLKVLIDFVPNHVARGYKSD
                     MLPKGIVDLGANDDKTKAFNAQNNFYY"
     gene            809..>1337
                     /locus_tag="PLO59_09380"
     CDS             809..>1337
                     /locus_tag="PLO59_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01358.1"
                     /translation="MISTLRLGLLVCCMALLNGCKNDIDITADYKETSIVYGLLNPSD
                     SVQYVKIYKTFLGDANAYEMAASTDSFYHQNVLDVTIERWKNNTLAGIIQCVRDSSIL
                     MDSGIFANAPNITYKTINNDSIYADSDYRLVIRNTKTGLITYATTSVIDKFFMYNSVE
                     AYVNMLQNPYKVIWFN"
BASE COUNT          426 a          226 c          240 g          445 t
ORIGIN      
        1 aaataataaa agttgttttg cgcattaaat gcttttgttt tatcatcatt ggcgcccaaa
       61 tcaactatac cttttggcag catatcgctc ttgtaaccac gtgctacatg attaggcaca
      121 aaatcaatca acacttttaa attattttta tgtgtgcgca taaccaaact ttcaaattca
      181 gccattcggt ttttaacatc tactgctaaa tctgggttta catcataata atctttaatc
      241 gaataggccg accctgccct gccttttaca acatcaggat catcggccac tataccaaaa
      301 gcactataat cggtaagggt tgcatgttca attatacccg tgtaccacaa gtgtgtaaag
      361 cccatgccct ttaaactcat taatgcggca tcattaatat cattaaactt accaacaccg
      421 ttttgcgcaa tggtgccata aggtataagg ttggtttgtt tattacccag caatcgggtc
      481 attacttgat agatgagtat tttatgattg gtaatttttt cggaggtggt atttgaatcg
      541 tttataattt cagtttgatt cataacttga ttggatttgt tgctacatgc cattgccaaa
      601 caactaataa atagcagtgg taaaaatatt ttcatagata aacttattgg ggtttaggcg
      661 cgtgctaatg tagaaattta tacaaagtgt aaaaattaca ctttgattta gttaagcggc
      721 aatgcttgta tttgtttggt aaagttttaa ggtaataatg ttgatttaaa agataaaatt
      781 aatttcggcc aaatttttaa aaacaggtat gatttcaact ttgcgcttgg ggctattggt
      841 ttgctgtatg gcattactaa acggatgtaa aaacgacatt gatattacgg cagattataa
      901 agaaaccagc attgtgtatg gcttgttaaa cccaagcgat agtgtgcaat acgttaagat
      961 ttacaaaacc tttttgggcg atgcaaatgc ttatgaaatg gctgcttcaa ccgattcgtt
     1021 ttaccatcaa aatgttttag atgtaactat tgaacgttgg aaaaacaaca ccttagccgg
     1081 cataattcaa tgcgtacgtg atagcagtat tttaatggat agtggcattt ttgccaatgc
     1141 cccaaacatt acatacaaaa ctattaacaa cgacagcatt tatgccgata gtgattaccg
     1201 attggtgata agaaacacca aaaccggcct gattacgtat gcaaccacga gtgttattga
     1261 caaatttttt atgtataaca gtgttgaggc ttatgtaaac atgttgcaaa atccttataa
     1321 agtaatttgg tttaatg
//
LOCUS       DAOMAA010000744         1335 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_123891_length_1335_cov_2.819531, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000744 DAOMAA010000000
VERSION     DAOMAA010000744.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123891_length_1335_cov_2.819531"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(225..>1335)
                     /locus_tag="PLO59_09385"
     CDS             complement(225..>1335)
                     /locus_tag="PLO59_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019987705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HQV01359.1"
                     /translation="EIKQFRNEARFIRALAYYYQLDLYGKGPFVTESDGVGSYDPREA
                     SRTELFNYVESELKAIEGLMIAPRQNEYGRADQAAAASLLARLYLNAEVYTGTARYND
                     CITYCNKVIAGGYTLDANYNWLFLADNHKSTEVIFPITSDGLRTQTYGGTTFIIHAAI
                     GGTMNDDDFGVNGGWFGLRTTKGLVSSFDNDSAVDKRYMFHIDGQNYDIAELKTFEDG
                     YAVNKWRNVDRNGIKGSDPAGNFVDTDFPLFRLADVYLMYAEATVRGGGGNMAQATAY
                     INALRDRAFGNTSGQVTTVDLPFLLKERARELYWECTRRTDLIRYGLFTGGNYLWPYK
                     GGSVAGNATSGHLNLYPIPAADLAVNKNLVQNPGY"
BASE COUNT          426 a          267 c          247 g          395 t
ORIGIN      
        1 attaatcatg agcagaagaa tgaaaagaaa ttttttcatc aaattttata gaatgatttc
       61 aaaaaacttt gcttgcaaat aaacaatgct acttggtatt tgttatcaaa aaatattttt
      121 gtttcagcac aaacttttaa aatatgcacc aacatataag tgaaaaaaag aaaagggcgg
      181 ttaactatgt aactgccctt taattaaaaa tgaaaaagga ttatttaata accgggattt
      241 tgaaccaggt ttttgttcac tgctaaatcg gctgcaggta tgggatataa atttaaatga
      301 ccggatgtag cattaccggc aacactacct cctttgtatg gccataaata attgccaccg
      361 gtaaataagc cgtaacgaat taaatcggtt ctgcgggtac attcccaata taattcgcgt
      421 gcgcgttctt ttagtaaaaa tggtaaatca acggtagtaa cctggccaga tgtattacca
      481 aaggcacgat cgcgtagcgc attaatgtaa gcagtagctt gtgccatatt gccgcctccg
      541 cctctaacag tagcttcggc atacatgagg taaacatctg ctaatctgaa caatgggaaa
      601 tcggtatcca caaaattacc ggcagggtcg ctgcctttta tgccattacg atcaacgtta
      661 cgccatttat taacagcata accatcttca aaagttttta gttcggctat gtcataattt
      721 tgaccatcaa tatggaacat ataccttttg tcaactgccg agtcgttatc aaacgagctt
      781 accaaacctt tggtggtacg taaaccaaac catccaccgt taacaccaaa atcatcatcg
      841 ttcatagtgc cgccaattgc tgcatgaata ataaaggttg taccaccata agtttgtgtg
      901 cgtaaaccat cggatgtgat aggaaatatt acttctgttg atttgtgatt atcggccaaa
      961 aacaaccaat tataattagc atccaatgta tagcctcctg caattacttt attacaataa
     1021 gtaatacaat cattatatcg tgctgtacca gtatatactt cggcattaag gtataaacgt
     1081 gcaagtaatg aagcggcagc agcttggtcg gcacggccat attcattttg gcgcggtgca
     1141 atcatcagcc cttctatggc ttttaattcc gattctacat aattgaaaag ttctgttcgg
     1201 cttgcttcgc gtgggtcgta agagccaacg ccatcgcttt cagtaacaaa tgggccttta
     1261 ccatataaat caagctgata gtaataggca agggcacgta taaagcgggc ttcgtttcta
     1321 aactgcttaa tttcg
//
LOCUS       DAOMAA010000745         1335 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_123892_length_1335_cov_2.815625, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000745 DAOMAA010000000
VERSION     DAOMAA010000745.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123892_length_1335_cov_2.815625"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..177)
                     /locus_tag="PLO59_09390"
     CDS             complement(<1..177)
                     /locus_tag="PLO59_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQV01360.1"
                     /translation="MISNRQLFLNHLAPTSPAPMILEIEAAQGCFMIDVNGKKYIDFI
                     SGIAVSGVGHRHPKI"
     gene            398..>1335
                     /locus_tag="PLO59_09395"
     CDS             398..>1335
                     /locus_tag="PLO59_09395"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HQV01361.1"
                     /translation="MLTETAYQTHTFKNGIKLIFQFNDSFVGHSAILVNAGTRDENTD
                     KEGLAHFIEHVLFKGTKKRKSIHILNRLEVVGGELNAYTTKEETCIHASYLTPYFERA
                     MELMIDIVFNAVFPPKEIIKEQEVVIDEIRTYLDTPAEQIFDDFESQVFAKHPLGSHV
                     LGTIESVSAFTQKDISHFTKQNYTTDKMVISVVGNYQFETVVATIEKHLHTIKATTTT
                     VKRNAFTKYKPTQVIVKKATEQAHFILGGKAYSFKDKQRIELVLLNNILGGPGMNSRL
                     NLSIREKYGYTYNIESNYNAYSDTGIFSLYLATDKKL"
BASE COUNT          467 a          230 c          226 g          412 t
ORIGIN      
        1 aatttttgga tgcctgtgcc ctacaccgct tactgcaata cccgaaataa agtctatata
       61 ctttttgccg ttaacgtcaa tcataaaaca accttgggct gcttcaattt caagtatcat
      121 aggcgcaggg cttgttggtg ccaagtgatt taaaaacaat tgcctgttac taatcatatt
      181 gcaaatgaaa tttgaaaaat ggatttaact aattaatttc ttacctctta ttttaaaaac
      241 tagcaacaaa ctgtgcttct gctcctttta aaggccatta taataaaccg tgtgcaacaa
      301 gcttttaata atttatagca acaaattttt actaaaaatt gcgtgctaat catttcaaac
      361 ttgttttata tattttaaaa aacaaatctt gcaggctatg ttaaccgaaa ccgcctatca
      421 aacgcatact tttaaaaatg gtattaaact tatttttcaa ttcaacgatt catttgtagg
      481 ccatagtgct attttagtaa atgccggtac ccgtgatgaa aacactgaca aagaaggttt
      541 agctcacttt attgagcatg tactttttaa aggaactaaa aagcgaaaaa gcatacatat
      601 actaaaccga ctggaagtgg ttggtggcga gttaaacgca tatactacca aggaagaaac
      661 ctgtattcac gcttcctatc ttacacccta tttcgaaagg gccatggaat tgatgattga
      721 cattgttttt aatgctgttt ttccgcctaa agaaattatt aaggaacaag aagtagtaat
      781 agatgagata cgaacttatt tagatacacc ggctgaacag atttttgatg atttcgaaag
      841 tcaggtgttt gccaaacatc cattaggcag tcatgtgctg ggcactattg aaagtgtatc
      901 tgcgtttact caaaaagata tcagccattt tacgaaacaa aattacacta ctgataaaat
      961 ggtaataagt gtagttggaa actatcagtt cgaaacagtg gttgctacca tcgaaaaaca
     1021 tttgcatacc ataaaagcaa caacaacaac tgtaaaacga aatgctttta ctaaatacaa
     1081 gccaacgcag gtaatagtta aaaaagctac agagcaagct cattttattt taggtggtaa
     1141 agcctattcg tttaaagata aacaacgaat agagttggta ttactaaaca atattttagg
     1201 aggcccgggt atgaattcgc ggcttaattt atcaatacgc gaaaagtacg gttacactta
     1261 taatattgag tcgaattata atgcgtatag cgataccggt atttttagtt tatatctggc
     1321 taccgataaa aaact
//
LOCUS       DAOMAA010000746         1335 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_123943_length_1335_cov_2.239063, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000746 DAOMAA010000000
VERSION     DAOMAA010000746.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123943_length_1335_cov_2.239063"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1335)
                     /locus_tag="PLO59_09400"
     CDS             complement(<1..>1335)
                     /locus_tag="PLO59_09400"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HQV01362.1"
                     /translation="HPSMKPFGLSFQSNNHTAQSNQPNMPTSTWYKNKPGIGDKVANE
                     LGGLTLFTQSDYQTLQKGNMRFVVSSIYPVEKGFVASHGLLGEKLIEKVMGIGANRIN
                     YIQKHNNYYADAKNEYDFLMQLNDVAINIDGQAFMYRTLTSGKDIEKLQSMYSNSNVI
                     INVISIEGGHALNCGLPGIKANTDDVLANVEKIRKWTHRPFFITLAHHFYNELCGHAR
                     SLHLPLQKQLKINQNQYLDTGINPLGFDVINAMLQNRDGCRILVDIKHMSRQSRLQYF
                     EIAKTKQVPIIASHAGVTGLHNPQGHETIKGASKKFMTDDINFYDDEIIAIAASDGLI
                     GIQLDERRMASNDALFEANTTDLTYRWEKWAELLWRQFFHIAQVLDNESMYAWDCICI
                     GSDFDGLINPIGPYWTAAETEELYKQLLKHASQTVPGYRWQFAANNASPKLI"
BASE COUNT          336 a          262 c          267 g          470 t
ORIGIN      
        1 ctatcagttt agggcttgca ttattagcag caaattgcca gcggtaacct ggcaccgttt
       61 ggcttgcatg cttaagtaac tgcttgtaca gttcttctgt ttctgcggca gtccagtaag
      121 ggcctatggg gtttatcaac ccatcaaagt ctgaacctat gcagatgcaa tcccatgcat
      181 acatgctttc attatctaat acttgcgcta tatgaaaaaa ctgacgccat aataattcgg
      241 cccatttctc ccatctatag gtaaggtcag ttgtgtttgc ttcaaacaag gcatcgttgc
      301 ttgccatgcg tctttcgtct aactgtatgc ctattaagcc atcgctggct gcaattgcaa
      361 ttatttcatc atcgtaaaaa ttaatatcat cggtcataaa tttttttgat gcgcctttta
      421 tggtttcatg gccttgtgga ttgtgtaaac ctgttacacc agcatggctt gctataatgg
      481 gtacttgttt tgtttttgca atttcgaaat attgtaaacg actttggcga ctcatgtgtt
      541 taatgtcaac taaaattctg catccatccc ggttttgcag catggcattt ataacatcaa
      601 aacccaatgg gtttataccg gtatctaaat attgattttg attaattttt aattgctttt
      661 gcagtggcag atgcaaactg cgtgcatggc cgcatagttc gttatagaag tgatgtgcca
      721 aggtgataaa aaagggacga tgtgtccatt ttctgatttt ttcaacattg gctaaaacgt
      781 catcggtatt tgctttaata ccgggcagtc cgcagttaag tgcatgacca ccttcaatac
      841 taattacatt aataatcaca ttgctgttgc tgtacatgct ttgcaatttt tctatatcct
      901 tacccgatgt aagcgttctg tacataaagg cttgaccatc tatgtttatg gcaacatcgt
      961 ttagttgcat taagaaatcg tattcgtttt tagcatctgc atagtagttg ttgtgttttt
     1021 gaatataatt tatcctgttg gctcctatgc ccattacttt ttcgataagt ttttcgccta
     1081 acaaaccgtg cgaagcaaca aagccttttt cgacagggta aattgacgat accacaaaac
     1141 gcatattgcc tttttgtaag gtttgataat cgctttgtgt aaataatgtt agcccaccca
     1201 gttcgttagc tactttatcg cctatgccgg gcttgttttt gtaccaggtt gatgttggca
     1261 tattgggctg attgctttgg gctgtatggt tgtttgattg gaagcttagc ccaaaaggtt
     1321 tcatggacgg atgac
//
LOCUS       DAOMAA010000747         1334 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_124047_length_1334_cov_3.546521, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000747 DAOMAA010000000
VERSION     DAOMAA010000747.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124047_length_1334_cov_3.546521"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="PLO59_09405"
     CDS             complement(<1..682)
                     /locus_tag="PLO59_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01363.1"
                     /translation="MGGGLYYEYLGINPVNGKYKYLVSAKVYRYCEPGSANLNNKLLL
                     GVYNEDPSNPNATSKTKYGQFNINRISLTFIQPPNPGDSCTFLPNVCVQEGYYSGIIN
                     LDASIGGYHLIADECCRNGNIVNINNPTGAGFAFYAFVPPTNIVNNSPVFALPPIPFL
                     CTNDTASMLNTAIDPDGDVLIYNFVRPLKGVSVSAPNPTPSPYPWPIPKVNYSAGHSV
                     FQPLGAAGG"
     gene            complement(971..>1334)
                     /locus_tag="PLO59_09410"
     CDS             complement(971..>1334)
                     /locus_tag="PLO59_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014019070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQV01364.1"
                     /translation="GKNKTERNAMAELSLKNVGLDNRMTHKPNELSGGQRQRVAVARA
                     LVNAPSIILADEPTGNLDSKTSVEIMGLFEEIHQKGNTIIVVTHEEDIARYAHRIIRI
                     KDGLIESDIQNKDYIRVR"
BASE COUNT          399 a          242 c          275 g          418 t
ORIGIN      
        1 ttccgcccgc agcccctaat ggttgaaaca cactatggcc ggccgaatag ttaactttgg
       61 gtataggcca agggtagggc gaaggggtag ggttgggtgc cgaaaccgat acacctttta
      121 atggacgaac aaaattgtaa attaacacat cgccatcagg atctattgcc gtgtttaaca
      181 tagaagctgt atcgttggta cataaaaacg gaatcggagg cagtgcaaat acgggcgaat
      241 tatttacaat gtttgttggt ggtacaaatg cgtaaaacgc aaaacctgca cccgtaggat
      301 tattaatatt tacaatattt ccattgcggc aacattcgtc agcaattaaa tgataaccgc
      361 ctattgatgc atctaaattt atgatgcctg agtaataacc ctcttgcaca caaacattgg
      421 gtaaaaaggt gcagctatcg cccggattag gtggctgtat aaaggtgagc gaaatgcggt
      481 ttatattaaa ctgaccatat ttggttttac tggtagcgtt tgggtttgat gggtcttcgt
      541 tataaacacc taacaataat ttgttgttta aattggcgct gcccggttcg cagtagcgat
      601 aaactttggc cgataccaga tatttgtatt tgccgtttac gggatttatt cctaagtatt
      661 catagtataa gccacctccc ataatatggg tagctttact tataaaagga atgcatagaa
      721 gaaaaaataa tacgaataag gagtactttt tttgcatgat atagtgtagt aaataaataa
      781 aactattagg gagaaaatgg gggcattgtg gatagtgcta ttttcatacc aatccaatcc
      841 ggtttgatta taaatgcaaa agtaagcatt gcaaaatatg cttaaaagca ctttaatatt
      901 aaatatctaa ccaataaaaa ggtaattaaa aacaattgtt ttaattattt ggcaataaat
      961 agttgattaa ttatcgtacc ctgatgtaat ctttattttg aatatccgat tcaattaaac
     1021 cgtctttaat tctgataatg cggtgtgcat agcgcgctat gtcttcttcg tgggtaacaa
     1081 caatgatggt atttcctttt tggtgaattt cttcaaaaag ccccattatc tctactgatg
     1141 ttttcgagtc gaggttaccc gttggctcat cagccaatat aattgagggt gcattaacca
     1201 aagcgcgcgc cacagctaca cgctgccttt gtccgcccga tagttcgttg ggcttatgtg
     1261 tcatacggtt atccaaccca acatttttta gcgatagctc tgccattgca tttcgctcgg
     1321 ttttgttttt gcct
//
LOCUS       DAOMAA010000748         1334 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_124073_length_1334_cov_2.839719, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000748 DAOMAA010000000
VERSION     DAOMAA010000748.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124073_length_1334_cov_2.839719"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="PLO59_09415"
     CDS             <1..327
                     /locus_tag="PLO59_09415"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HQV01365.1"
                     /translation="NLAGCRKVYELPVTVAPFWIPNAFSPNDDNRNDLFFNSNYVMDV
                     ISFEMIIRNRWGQVVYETKDFNQPWDGRGNDESIAPEGTYVYDINIRAKTGLDYNFKG
                     TVNLIR"
     gene            complement(363..>1334)
                     /locus_tag="PLO59_09420"
     CDS             complement(363..>1334)
                     /locus_tag="PLO59_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01366.1"
                     /translation="QVNEGSYWEHNHYILCCKQTLKKAALELNLSYNEFETRINNLRK
                     RVLEARAKRIAPAFDDKKITAWNALTITAFVDAHFTIGNFDYLQSALQTAEFICDKLW
                     IENNLYHTFKNNKATINGCLDDYTFTAQAFIKLYQATGNSQWLLKAHRLIETTLHQFA
                     SQNALCYYNHKNANLFNRPLQINDDVIASGNSILAQCLFQLGQYFGNNGWLDAATQMV
                     QEVVSEIEKYPAGYAYWLQLIQWMQAPYYQICFAGPDAVKLHQAFFKLYNFSYLPILV
                     TKPETHPWSLNKFQNDNVIYICKHGQCFAPLSTIQEALSFLQNNNSL"
BASE COUNT          419 a          221 c          248 g          446 t
ORIGIN      
        1 aatttagcgg gttgccgtaa agtttatgaa ttgcctgtaa ctgttgcacc attttggata
       61 cccaatgcat tttcgccaaa tgatgataac agaaatgatt tgtttttcaa ctcaaactat
      121 gtaatggatg ttatcagttt cgagatgatt atcagaaacc gatggggaca agtggtatat
      181 gaaaccaaag attttaacca accttgggat ggccgtggta atgatgaaag catagctcct
      241 gaaggtactt atgtatatga tattaatatc agagctaaaa ccggattaga ctacaacttt
      301 aaaggtaccg taaaccttat caggtaatta aaaggtatga ttaaaaacaa caacagcctc
      361 aattagaggc tgttgttgtt ttgtaagaaa cttaatgcct cttgtatggt tgataaaggt
      421 gcaaagcatt gcccatgctt acatatgtaa attacattgt cgttttggaa tttatttaac
      481 gaccaaggat gtgtttcggg cttagtaacc aatataggta aataactaaa gttatatagt
      541 ttaaagaatg cttggtgaag cttgactgca tcaggccctg caaaacaaat ttggtaataa
      601 ggtgcttgca tccattgtat taattgcagc caatatgcat agcctgccgg atatttttca
      661 atttctgaaa ccacctcctg caccatttgt gtagctgcat ctaaccagcc attattgcca
      721 aaatattgtc ctaactgaaa taagcattga gccagaatgc tgttgcccga agcaattaca
      781 tcatcattaa tttgtaaggg cctgttaaac aaatttgcat ttttatgatt gtaataacaa
      841 agcgcatttt gggatgcaaa ttgatgtaag gttgtttcaa ttaaacggtg tgcttttaac
      901 aaccattgac tgttgccggt tgcctgatac agttttataa aagcttgtgc cgtaaaagta
      961 taatcatcta aacaaccgtt tatagtggct ttgttatttt taaacgtatg atataaatta
     1021 ttttcaatcc acaacttatc gcaaataaat tcagcagttt gtaatgcgct ttgtaagtaa
     1081 tcaaaattgc caattgtaaa atgggcatca acaaaagcgg ttattgttaa ggcattccag
     1141 gcggtaattt ttttatcatc aaaagcaggt gctattcgtt tggcgcgtgc ttccaaaact
     1201 ctttttctaa ggttgttaat ccttgtttca aattcattat acgaaaggtt taattcaagt
     1261 gcagcttttt ttaacgtttg tttgcaacaa agtatgtaat gattgtgctc ccaataacta
     1321 ccttcgttta cttg
//
LOCUS       DAOMAA010000749         1330 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_124775_length_1330_cov_3.053333, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000749 DAOMAA010000000
VERSION     DAOMAA010000749.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124775_length_1330_cov_3.053333"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="PLO59_09425"
     CDS             complement(<1..224)
                     /locus_tag="PLO59_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQV01367.1"
                     /translation="MKLHTIETGRFKLDGGAMFGVVPKSIWESLNPADANNMCTWALR
                     CLLVETGNKLVLIDNGIGNKQDAKFFSYYY"
     gene            complement(231..>1330)
                     /locus_tag="PLO59_09430"
     CDS             complement(231..>1330)
                     /locus_tag="PLO59_09430"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV01368.1"
                     /translation="IPNEHKNYLVINQPGLYAVVVKYGCCNDGYYSLNIDSCSTGYMV
                     KPIVTPNVYICDSSSTTLTATGAINYRWYKTDSSTTILSTDSFYTTPILTATDTFYVA
                     AYNGTCESSRTRIIVSKTTKPAAPVTTNYFQCNNDALIIFPGNGFSFNWYTDSNLMPF
                     KTNSSFLHLPNPVTDTIYVAVNNYQCNSYFSPIIITVGSQTPQVVLTGDTTIYPFANA
                     TYFYSNIANNNIQWIVNGGTYTLYPDSIVVQWGNSPNASIAYIETDLYGCVSDTVYFP
                     VNVSVIHALQNKHVSDISIYPNPVKDLLFIDSFKNHANGTTLTCFNSAGQKIHEQKLQ
                     NKLAVVNTKTWPKGLYYIKIEPIHFYQTIIK"
BASE COUNT          402 a          218 c          262 g          448 t
ORIGIN      
        1 aaatagtagt agctaaaaaa tttagcgtct tgtttattgc caattccatt atcgatcaac
       61 accagcttat taccggtttc aactaacaaa caacgtaatg cccaggtgca catattattg
      121 gcatcggccg ggttaagcga ttcccaaatt gattttggca ctacgccaaa catggctcca
      181 ccatccagtt taaaacgtcc tgtttcaata gtatgcagtt tcatagtttt ttatttgatt
      241 atagtttgat aaaaatggat gggttctatt ttaatataat ataaaccttt gggccatgtt
      301 tttgtattta caacagccag tttattttga agtttttgtt catgtatttt ttgacctgca
      361 ctgttgaaac aagttaatgt tgtgccattt gcatggtttt taaacgaatc aataaacaac
      421 aaatctttta caggattagg gtatatactt atgtccgata cgtgtttatt ttgtaatgca
      481 tgtattaccg aaacgtttac aggaaaataa actgtatcac ttacgcagcc atataaatcg
      541 gtttcaatat atgcaataga agcattggga ctattgcccc attgcactac tatcgaatcg
      601 ggatagagcg tataagtgcc accatttaca atccattgta tgttgttgtt tgcaatgttt
      661 gaataaaagt aagttgcatt ggcaaaagga taaattgtgg tatcgcctgt taatactact
      721 tgtggtgttt ggctgcctac cgtaattata atgggcgaaa aataactgtt acattgataa
      781 ttgttaactg caacatatat agtatcagtt acagggttgg gcaagtgtaa aaacgagcta
      841 ttggttttaa atggcattaa gttactatcg gtgtaccagt taaagctaaa cccattaccc
      901 ggaaaaataa ttaatgcatc gttattacat tgaaaatagt tggtggttac cggtgctgca
      961 ggcttggttg tttttgaaac aattatgcgt gtacggctgc tttcgcaagt accattgtaa
     1021 gcggctacat aaaatgtatc ggttgcagta agtatgggcg tagtgtaaaa ggaatcggta
     1081 cttaatatgg ttgttgacga atcggtttta taccagcggt aattaatggc accggttgcg
     1141 gttaaagtgg tgctactcga atcgcatata taaacgtttg gtgttactat gggtttaacc
     1201 atataaccgg tactgcaact atcaatattt aaactgtaat aaccatcgtt gcaacaacca
     1261 tatttaacca caactgcgta aagtccgggt tggtttatta ccaaatagtt tttatgctca
     1321 ttgggtatga
//
LOCUS       DAOMAA010000750         1330 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_124776_length_1330_cov_3.036078, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000750 DAOMAA010000000
VERSION     DAOMAA010000750.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124776_length_1330_cov_3.036078"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1275
                     /locus_tag="PLO59_09435"
     CDS             <1..1275
                     /locus_tag="PLO59_09435"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HQV01369.1"
                     /translation="VSHLGISNLNQLQNDVLQATYQHIHVIAPTGSGKTVAFLLPVLS
                     SLDVNNKALRALIVVPMRELALQIAQVFTQMQTGYKCVCCYGGHNMQAEVRSLQQTPT
                     LIIGTPGRLQKHISLNNIDTSHLKVLVLDEFDKSLEIGFAESMQTIVASCNNTKIILT
                     SATAMPALPDFLKHYNWHYIRSEHQIPEITCYSAKVLGDDKAEALLLMLNSFAAEPAI
                     VFCNHRDAAQRLSSFMQTHKISHSLYHGKLEQQQREMAWIQFANGSNRLLITTDLASR
                     GLDIARVKHIIHYQLPQTAETNTHRNGRTARMQATGNAYYLLDKGDVMPLFITAKLNP
                     FQWPQQMQPWPKIQWVTLYIASGKKDGLSKTDVLGVLYKKASFQKNDLGLMSVFDHFI
                     LIAITANLASIAWQTLTNYKVKNKKVKVSLLP"
BASE COUNT          431 a          271 c          253 g          375 t
ORIGIN      
        1 gtttcacact taggtatcag caacctcaat caattacaaa atgatgtact gcaagctaca
       61 tatcaacata tacatgttat tgcaccaacg ggcagtggta aaacagtggc ttttttactg
      121 cctgtattaa gtagtttaga tgtaaacaat aaagcgttgc gggcacttat tgtagtacct
      181 atgcgcgagt tggcattaca aatagcgcag gtatttacac aaatgcaaac cggttataaa
      241 tgtgtttgtt gttatggtgg ccataacatg caggccgagg tgcgtagttt acagcaaacc
      301 cctacactta ttattggcac accgggtcgt ttgcaaaagc acatcagcct aaataatatt
      361 gatacatcac acttaaaagt actggtgtta gacgagtttg ataaatcgtt ggaaattggt
      421 tttgccgaat caatgcaaac tattgtcgcc tcttgcaaca acaccaaaat catacttaca
      481 tcggcaacag ctatgcctgc cttgcctgat tttttaaaac attataactg gcactatata
      541 cgtagcgaac atcaaatacc tgaaataacc tgttatagtg ccaaagtatt gggcgatgat
      601 aaagccgaag cactgttact tatgcttaat agctttgcag ccgaaccggc cattgttttt
      661 tgcaaccacc gcgatgcagc ccagcggctt agttctttta tgcaaacaca taagataagc
      721 catagtttgt accacggtaa attagagcaa caacaacgcg aaatggcctg gattcaattt
      781 gcaaacggca gcaaccgctt gttaattacc accgatttgg cttcgcgtgg tttagatatt
      841 gcccgggtaa aacacatcat acattatcag ttgccacaaa ctgcagaaac caatacacac
      901 cgcaatggcc gcaccgcacg catgcaagcc acaggtaatg cctattattt gttagataaa
      961 ggcgatgtta tgccactgtt tattactgca aagcttaacc catttcaatg gccgcaacaa
     1021 atgcaaccat ggcctaaaat acaatgggtt actttataca tagcttccgg taaaaaagac
     1081 gggctaagca aaaccgatgt tttaggagtg ttgtataaaa aagcatcatt tcagaaaaac
     1141 gatttggggt taatgagtgt tttcgaccat tttattttaa tagccataac agccaatttg
     1201 gcaagcattg cttggcaaac acttacaaat tataaagtaa aaaacaaaaa agtaaaagtg
     1261 agtttactcc cataatgggt ttgattttgt agataaacct ttacaattga tagttgcttt
     1321 attgtcgtaa
//
LOCUS       DAOMAA010000751         1330 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_124781_length_1330_cov_2.964706, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000751 DAOMAA010000000
VERSION     DAOMAA010000751.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124781_length_1330_cov_2.964706"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /locus_tag="PLO59_09440"
     CDS             <1..963
                     /locus_tag="PLO59_09440"
                     /inference="COORDINATES: protein motif:HMM:NF036799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HQV01370.1"
                     /translation="NKIDLPAQLRTATVSSKSQQRYAYKNRISNYLIAQGFNEIWSNS
                     LTKQAYADMCDAKPTPVVLLNPLSTDLNSLRTNLLFSALEAAQYNSNRKNSDLKLFET
                     GNGYVLDNGYKETNFLSLILTGQRQTGSRFVTPAQDIFYLKALVQNVFKSVCVSFKEM
                     VQTATSNYAQLIHLNVGKTLVASYGMLQKSLTKQFDLEGAVYFAEINLDVLFQFAGKA
                     TMAITEVPKYPEVKRDLSMLLEKGVAYNELEKLAFETERKYLKQVNLFDVYEGKNIPP
                     DKKSYALSFTLYDAEKTLQDADIDKVMKKLMAAFEQKLGAEIRK"
     gene            complement(986..>1330)
                     /locus_tag="PLO59_09445"
     CDS             complement(986..>1330)
                     /locus_tag="PLO59_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01371.1"
                     /translation="YASNTQLVIDKNFTRTILDAQLQPGKFVADGNALFIATFGQTDP
                     DYAARTENYKNNYKANNISLSGLIIHTDKMVPCQKPFPYIIRTHETGQIISDKKIIKA
                     VKQLQKKLKKGF"
BASE COUNT          437 a          241 c          257 g          395 t
ORIGIN      
        1 aataaaattg atttgcctgc acaactacgc acagccacag ttagcagtaa aagtcagcaa
       61 cggtatgcat acaaaaaccg tataagcaat tacttaattg cacagggctt taacgaaata
      121 tggagcaact cattaaccaa gcaggcttat gccgatatgt gcgatgcaaa accaacaccc
      181 gttgtgctat taaatccatt aagcaccgat ttaaacagct tgcgaaccaa cttgttattt
      241 agcgctttag aagcagcaca gtataacagt aaccgcaaaa attctgattt gaaattattt
      301 gaaacaggta atggctatgt gcttgataac ggatataaag aaaccaattt tttaagcctg
      361 atacttaccg gacaaaggca aaccggcagc aggtttgtta cacctgcaca ggatatattt
      421 tacttaaagg cattggtgca aaacgtgttt aaaagtgtat gtgtttcgtt taaagaaatg
      481 gtacaaactg ctacttcaaa ctatgcacaa cttatccatt taaacgtagg caaaacgctg
      541 gtggcaagtt atggcatgtt acaaaaatcg cttaccaaac aatttgattt agagggtgcg
      601 gtttattttg ctgaaattaa tctggatgta ttattccaat ttgccggcaa agcaacgatg
      661 gccattaccg aagtacccaa ataccccgaa gtaaaacgcg acttaagtat gcttttagaa
      721 aaaggtgttg cttataatga attagaaaaa cttgcatttg aaaccgagcg caaatattta
      781 aagcaggtta atttgtttga tgtttatgaa ggcaaaaaca taccacccga taaaaaatct
      841 tatgcactga gttttacttt atacgatgcc gaaaaaacgt tgcaagatgc cgatattgat
      901 aaagtaatga aaaagctgat ggctgctttc gaacaaaaat taggtgccga aatcagaaaa
      961 taaaatcacc acttcatcac tcgtattaaa aacctttttt tagttttttc tgcaactgct
     1021 taacggcttt gattattttt ttatcgctga taatctgacc ggtttcatgt gtgcgtataa
     1081 tgtatggaaa cggcttttga caaggcacca ttttatcggt atgaattatt aagcccgaca
     1141 agctgatgtt gtttgccttg taattgtttt tataattttc ggtacgtgct gcataatccg
     1201 ggtcggtttg gccgaatgtg gcaataaaca gggcattgcc atcggcaaca aatttgcccg
     1261 gctgcaattg cgcatcaagt atggtacggg tgaaattttt atcaattact aattgtgtat
     1321 tgctcgcata
//
LOCUS       DAOMAA010000752         1329 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_124997_length_1329_cov_2.682889, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000752 DAOMAA010000000
VERSION     DAOMAA010000752.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124997_length_1329_cov_2.682889"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="PLO59_09450"
     CDS             <1..256
                     /locus_tag="PLO59_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019668556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HQV01372.1"
                     /translation="VAYKELNGFGITPDKSKLVIDCVRAEGVIGTHLVQYQSEIDEIT
                     LSHKAFNRQGFAEGALLAANWIFGKKGFFTMQDMLHLNLR"
     gene            337..>1329
                     /gene="lepB"
                     /locus_tag="PLO59_09455"
     CDS             337..>1329
                     /gene="lepB"
                     /locus_tag="PLO59_09455"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HQV01373.1"
                     /translation="MFSNKEKTKKPKSKAREWFDAIIFAVVAATLIRTFLLEAFTIPT
                     PSMEETLMVGDFLFVSKVNYGPRIPQTPLSLPFVHNEILGFKSYSEAIKLDYKRLPGF
                     ATIKNNDIVVFNYPGMPGEPDDTRPVDKKTHYIKRCIAIAGDSIKIIDGEVYVNGKPS
                     QFPKRAQKIYRVYSRAFMFDESTLYNLGITNWDMQQFNDNILMLTPDAAVQLTQLPNI
                     DSVVAVFQPKEYPDSRIFCYDSLHLYNGDNFGSIYVPKAGDVVPINSATYSIYKRAIT
                     AYEHNSLEEKAGKYYINGKEITSYTFKMNYYFMMGDNRYNSLDCRYWGFVPEANI"
BASE COUNT          432 a          236 c          250 g          411 t
ORIGIN      
        1 ggttgcttat aaagaattaa acggatttgg tatcacccct gataaaagca aacttgttat
       61 agattgtgtg cgtgccgaag gtgttatagg aacacatctg gtacaatatc aatcagaaat
      121 tgacgaaata accttatcac ataaagcatt taacagacaa ggttttgccg aaggcgcttt
      181 attagctgcc aattggattt ttggtaaaaa aggatttttt acgatgcagg atatgctgca
      241 tttaaattta cgttaaccgc gtaaccctgt taactaaatt aactccatat tgcaagcccc
      301 acttttaatt aactgttatt gtaactgtta cactatatgt tttcaaacaa agaaaaaacc
      361 aagaaaccaa agagtaaagc acgcgaatgg tttgatgcca ttatttttgc cgtagttgct
      421 gctactttaa tccgtacttt tttgctcgaa gcttttacca tacctacacc aagtatggaa
      481 gagacactta tggttggcga ttttttattc gtaagcaagg ttaattatgg gccacgcata
      541 ccacaaactc cgctttcatt accttttgta cataatgaaa ttttaggctt taaatcatat
      601 agcgaggcta taaaattaga ttacaagcga ctgccgggct tcgctaccat taaaaataac
      661 gacattgttg tatttaatta cccgggtatg ccgggcgaac cggatgatac acgccctgtt
      721 gataagaaaa cacactatat taaacgatgc attgccattg caggcgatag cataaaaata
      781 attgatggtg aagtgtatgt aaacggcaaa cccagccaat ttcctaaacg cgcacaaaaa
      841 atttacagag tttattcaag agcctttatg tttgatgaaa gtactttgta taacctgggt
      901 attacaaact gggacatgca acagtttaat gataatattt taatgcttac acccgatgct
      961 gctgttcaat taacgcagtt gccaaatatt gatagtgtgg tggctgtgtt tcaaccaaag
     1021 gagtatcctg atagccgtat tttttgttat gattccttac acttatataa tggcgataat
     1081 tttggatcta tttatgtacc aaaggcaggc gatgttgttc ctataaacag tgcaacatat
     1141 agcatttata aacgtgccat tactgcctac gaacacaaca gtttagaaga gaaagctggc
     1201 aagtattata ttaatggcaa agaaattaca agctatactt ttaaaatgaa ttattacttt
     1261 atgatgggtg ataacaggta taactcatta gattgcaggt actggggttt tgtgcccgag
     1321 gccaacatc
//
LOCUS       DAOMAA010000753         1328 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_125175_length_1328_cov_3.073056, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000753 DAOMAA010000000
VERSION     DAOMAA010000753.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125175_length_1328_cov_3.073056"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="PLO59_09460"
     CDS             <1..232
                     /locus_tag="PLO59_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01374.1"
                     /translation="KYFLRHSLLTYSLIFTFFFAFSVGFSTSNFGSLVRYKIPIIPFF
                     VCSLYIIDYLHKLDKQNAVQKIKQFTPIGAKI"
     gene            239..1102
                     /locus_tag="PLO59_09465"
     CDS             239..1102
                     /locus_tag="PLO59_09465"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HQV01375.1"
                     /translation="MINRLLFPLKQLFTLSFFDSRLAALTKGKSMDNLFVKLLPSNLQ
                     YSKNDVRLTEVNGVKYQLYLNQWMEYALYFGIQTEDKAALYKLIKPDFHVIDVGVNFG
                     ETLLNIAKLTGANGHVIGFEPMPAIFKKCQDNMKLNTFANITLEPFALSDVEATVQIT
                     DPLNGNSGGTYISSAFTNSSLQVQAITLDDYLNRHSINKIDLIKIDVEGYELHVLQGA
                     KQTLLKHKPVLYIELCDAHLKRAGNSSLALIVFLQALNYGIKKADTGETITSLNMQQY
                     QWIDICCTPHA"
BASE COUNT          421 a          235 c          237 g          435 t
ORIGIN      
        1 taaatacttt ttgcgtcact cattactaac ttactcgctg atttttactt ttttcttcgc
       61 tttttcagtt gggttttcaa cttcgaattt cggcagttta gttaggtata agattcctat
      121 tattccattt tttgtttgct cactttatat aattgattac ctgcataagc tggataaaca
      181 aaatgcagta caaaagatta aacagtttac acctattggc gccaaaattt aatttagtat
      241 gattaaccgt ttgttgtttc cgctaaaaca attgtttaca ctttcttttt ttgattcgag
      301 gttggctgca ttaaccaaag gcaaaagtat ggacaacctt tttgtaaagc tattgccttc
      361 caatcttcaa tattcgaaaa acgatgtaag gcttaccgaa gtaaatggtg taaagtatca
      421 gctctattta aatcaatgga tggagtatgc tttgtatttt ggtattcaaa ccgaggacaa
      481 agcagcattg tacaagctta ttaagccgga ctttcatgta attgatgtgg gcgtaaattt
      541 tggcgaaacc cttttgaata ttgcaaaact aaccggggca aatggccatg taattggttt
      601 cgaacctatg cctgctattt ttaaaaaatg tcaagataat atgaaactta acacttttgc
      661 caacattaca ttagagccct ttgctttaag cgatgttgaa gcaacggttc aaataaccga
      721 tccattaaat ggcaacagtg gcggcactta tatttcaagt gcgtttacaa atagcagttt
      781 gcaagtacaa gccattacct tagatgatta tttaaaccgc cattcgataa ataagattga
      841 cctaataaag attgatgtcg aaggttatga actacatgta ttgcaaggtg caaaacaaac
      901 gttgctaaaa cacaaacctg tactttacat tgaattatgt gatgcccacc taaaacgtgc
      961 gggcaatagc agcctggcat taattgtgtt tttacaagcc ttaaactacg gcataaaaaa
     1021 agccgataca ggcgaaacca ttacatcgct taatatgcaa caatatcaat ggattgacat
     1081 ttgctgcact ccgcatgctt aaggttatga tttagtactt aaggcgcttt actggttaat
     1141 gagaaagtta ttgcatgata cgatatggct ccagacatga aaaaaaaatg gagaccgttt
     1201 tcttttaaaa gcaatctcca ttttaatcca tgctaaaatt agcaacattt actgtttaac
     1261 aaactttttt acggtgcgat gtgttgccgt agttaagctg aggtgataca tacccggcat
     1321 aagtgtag
//
LOCUS       DAOMAA010000754         1327 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_125398_length_1327_cov_2.408019, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000754 DAOMAA010000000
VERSION     DAOMAA010000754.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125398_length_1327_cov_2.408019"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1097)
                     /locus_tag="PLO59_09470"
     CDS             complement(<1..1097)
                     /locus_tag="PLO59_09470"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HQV01376.1"
                     /translation="MDFLSKTYFNHTGLDYLLAILILISAYALRKIFTYLFTTLLFKV
                     FHKYCAGVGQNKLTELLQKPFSVLVFLVSLYFAMAELNYPASWNTSLAKTSLPTIIFI
                     SCFKIALLASLIMMAVNLTEYVGLLLLYRAQNTASKTDDMLVPFIKESLKIIFIVLGV
                     FVLLSKVFHVHLTAILGGAGIAGLAIALAAQETLGNLLGSFTIFLDKPFVVGDLVRIG
                     AIHGRIEHIGFRSTRIRTLEKSYVTIPNKKMVDAELDNLTERTLYRVQFKLDFKYNSN
                     PDSIRAFNIEIKNYLLNNPNIDENVSVYLHEMTSSALQVIIIYFVKTNDWEEHIVVKE
                     ATIYNILETAKTLQLELAFPSSSIYIESANN"
BASE COUNT          469 a          233 c          221 g          404 t
ORIGIN      
        1 taattgtttg ccgattcaat gtaaatagaa gagcttggga aagccaactc taactgaagt
       61 gttttagcag tttcaagtat gttgtaaatg gtagcttctt taaccacaat gtgttcttcc
      121 caatcgttgg tttttacaaa atatataatg attacttgca atgcactgct tgtcatttca
      181 tgtaaataca cacttacatt ctcatcaata tttggattgt taagcaggta gtttttaatt
      241 tcaatattaa aggccctgat tgaatcggga ttggagttgt atttaaaatc taatttaaac
      301 tgcacccgat ataatgtacg ttcggttaaa ttatccaact cggcatcaac cattttttta
      361 ttgggtatgg taacgtaaga tttttcaagt gtacgtatgc gtgtacttct aaaaccaata
      421 tgttcaatac gtccatgtat ggccccaata cgaaccaaat cgccaacaac aaatggttta
      481 tctaaaaata tggtaaacga acctaacaag ttgcccagtg tttcctgagc tgcaagcgct
      541 atggctaaac cggcaatacc cgcaccgcct aaaattgctg ttaagtgtac atgaaatacc
      601 ttactaagca atacaaaaac acctaatacg atgaatatga tttttagaga ttcctttata
      661 aacggaacca gcatatcatc tgtttttgat gcagtgtttt gcgcccggta tagcaataaa
      721 agacctacat attcggttag gtttacagcc atcatgatta acgatgccag taatgcaatt
      781 ttaaaacagc ttataaaaat gattgtgggt aatgatgttt ttgccaatga agtattccaa
      841 ctggccgggt aatttaactc ggccattgca aaatacaacg acactaaaaa aaccaatacg
      901 ctaaatggtt tttgcaacaa ttcggttaac ttgttttgcc caacgcctgc acaatactta
      961 tgaaagacct taaatagcag ggttgtaaat aagtaggtaa atattttacg taatgcatag
     1021 gcagaaatta aaataagtat ggctaacaaa tagtctaaac cggtgtgatt gaaatacgtt
     1081 ttacttaaaa aatccatagt tgaaacaggc aggtattaat tacaaaacag gtaagccgct
     1141 tttctttatc attaaaattt aaacgcacat gttggcgaaa taagaaatta ttatttttgt
     1201 gatactactt aaatcaaaac tataactcaa attttagtaa acatggctac taaaaaatcg
     1261 aaagcgccag taaaatcaaa aaagaaaacc accaatgatg agctgcacaa gccatcaaaa
     1321 atgaaac
//
LOCUS       DAOMAA010000755         1326 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_125498_length_1326_cov_4.029898, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000755 DAOMAA010000000
VERSION     DAOMAA010000755.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125498_length_1326_cov_4.029898"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1248)
                     /locus_tag="PLO59_09475"
     CDS             complement(<1..1248)
                     /locus_tag="PLO59_09475"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HQV01377.1"
                     /translation="MQTVLAQLTVTLTPTNYNGYNISCFGKRDGSIAATVTAGGTGSY
                     TYEWSVDQTTPTVTGLAAGYYRVKVIDANGESAETEITLTQPEVLNLDKAVHIYSNSY
                     NVTCHFCADGIVNTQPVNGIAPYSYIWSDGGATQNRTGIDAGSYLVMVTDANGCTAAQ
                     DVAVTAPDRNDWTMTGNAGTNPSNQFIGTTDNKDLVFKTNNSERLRVNANGGVKINSL
                     SGNGTQLLLVDDNGLLLKQNPNIPIGLSTIWMTNGNLTTANHYIGTNNTQPLIFKTDV
                     NASATLGEKMRIMPDGKVGIGTNNPDVLFHIMGNKLLNPSQQPVALMKIESESPNTCG
                     LVFENIPAMGSAKQGAKFQLTGDGVFSIWNTSSTGSLGGGKPCIAIKDGTTVAGKVFI
                     GGLPSEILNPQTGSINYRLYVMGG"
BASE COUNT          348 a          288 c          252 g          438 t
ORIGIN      
        1 accacccatt acataaagtc tgtaattaat gctgcccgtt tgtggattta atatttctga
       61 tggcagtccg ccaataaaaa ctttgccggc aaccgtagtt ccatccttaa ttgcaatgca
      121 aggttttccg ccacccaacg agcctgtact tgatgtattc cagattgaga aaacaccatc
      181 acctgttaat tgaaacttcg caccttgttt tgcacttccc attgcaggaa tattttcaaa
      241 gactaatcca catgtgtttg gagactcgct ttcaattttc ataagagcca cgggttgctg
      301 cgatgggttt aataatttat tacccataat atgaaataac acatcggggt tattagttcc
      361 aataccaact ttaccatcag gcataatacg catcttttct ccaagggttg cgcttgcatt
      421 tacatccgtt ttaaatatta atggttgggt attatttgta ccgatgtaat ggtttgctgt
      481 ggttaaattt ccgttagtca tccaaatggt tgatagccct attggaatat ttggattttg
      541 ttttaaaagc aatccgttat catcaaccaa cagtaattgt gtaccatttc cactcaaact
      601 atttattttt actcccccat tggcattaac cctcaacctt tcgctattat tggttttaaa
      661 taccaaatcc ttattatcgg tagtaccaat aaactggttg cttgggtttg tgccggcatt
      721 accggtcatt gtccaatcat tacggtcggg tgcggttact gctacatctt gcgctgctgt
      781 acaaccattg gcatcagtta ccatcactaa ataacttccg gcatctatgc ctgttcggtt
      841 ttgtgtagca ccgccatcgc tccatatata cgagtaaggc gcaataccat ttacaggttg
      901 tgtatttaca ataccatcag cacaaaaatg acacgttaca ttgtaactgt tactataaat
      961 gtgcacagct ttatctaaat ttaatacttc gggttgggta agtgttattt ctgtttcggc
     1021 actttcgcca ttcgcatcaa taactttaac acgataatat cctgcagcaa gccctgttac
     1081 ggttggtgta gtttggtcaa cactccactc gtaagtatat gaacccgtac cacctgccgt
     1141 taccgttgct gcaatgcttc catcgcgttt gccaaaacag gatatgttat atccgttata
     1201 gttagttggt gttagcgtta cggtaagctg cgctaacacc gtttgcatgt taataacaag
     1261 cgctgctaaa acaaataatt tgcggggggg gggggagtca tggcttatgg cggtttagag
     1321 gtttat
//
LOCUS       DAOMAA010000756         1326 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_125544_length_1326_cov_2.898505, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000756 DAOMAA010000000
VERSION     DAOMAA010000756.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125544_length_1326_cov_2.898505"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1289)
                     /locus_tag="PLO59_09480"
     CDS             complement(<1..1289)
                     /locus_tag="PLO59_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01378.1"
                     /translation="MLRLRIALLFLMVGSFNIQAQFYNGTQMSFGKNRVQYQAFEWQF
                     FQFKKFDTFFYKGGRELAEYTGRTADAEIGEIEKLFDYQTDDRLQFIIFNKLSDLKQT
                     NLGLEAETQLNNTGGITRIFGNKILIYFDGNHAHFRQQIRAGIARVLFEQLMYGGSVK
                     DRLQSSILLNIPDWYISGLSMWVAKGWTTDDENRMRDGILSGKYKKLNSLATTDAAFA
                     GQSMWNFVDKVYGSASIANMLYMTRLNRNIESGFNFVIGATLKQTTNAWRIFYEKRYL
                     NNDNGKQTTTGKPLTIKSKNGVFINQVKLSPDGKKISYVTNEMGKYKVYLYDIKTKKR
                     KRIAKGGYRSLVQETDVSFPVVAWHPTGKSVTYIKEWKGNLWLSNYQLKGRKTDNSKL
                     LYFEKVNDFSYADDGQSLVMSATQKGQSDIFIWNIRA"
BASE COUNT          377 a          272 c          231 g          446 t
ORIGIN      
        1 cttgctctga tattccaaat aaaaatatcc gattgtcctt tttgtgttgc actcattacc
       61 aaactttggc catcatcggc ataactaaaa tcgtttactt tttcgaaata aagtaattta
      121 ctgttgtcgg ttttacgtcc ttttaattga taattgctta gccataaatt gcctttccat
      181 tccttaatat aggttactga tttgccggta ggatgccatg ccacaaccgg aaacgataca
      241 tcggtttctt gcaccaaact gcggtaacct cctttagcta tacgcttgcg tttttttgtt
      301 tttatatcgt acaaataaac tttgtacttg cccatttcat tggttacata gctaatcttt
      361 ttgccatcgg gcgatagttt tacctgatta ataaacacac cattttttga cttaatggtt
      421 agtggcttgc ccgtagttgt ttgctttcca ttatcgttat taagatatcg tttttcgtaa
      481 aaaatacgcc aggcattagt ggtttgttta agggttgctc caattacaaa attaaatccg
      541 ctttcgatgt ttcggtttaa gcgtgtcatg taaagcatat tggcaataga tgcactgcca
      601 tatactttat caacaaagtt ccacatgctt tggcctgcaa aagcggcatc ggtagtggcc
      661 aaactattaa gctttttata tttccctgat agtatgccat cgcgcatgcg gttttcgtca
      721 tcggtagtcc agcctttagc tacccacatt gacaaaccac ttatatacca atcggggata
      781 tttaaaagta tagaactttg taaacggtct ttaacgctgc caccatacat aagttgttca
      841 aacaatacgc gtgcaatacc ggctctgatt tgctggcgga aatgcgcatg gttgccatca
      901 aaataaatta aaattttatt gccaaaaatg cgtgtaatgc cgcctgtatt atttagttgt
      961 gtttcagctt ctaaacctaa attggtttgc tttaagtcgc tcagtttatt aaatataata
     1021 aactgtaaac gatcatcggt ttggtaatcg aacagttttt ctatttcacc aatttcagca
     1081 tcggctgtac gtccggtgta ttcggctaac tcgcgcccgc ctttataaaa aaaagtatcg
     1141 aactttttaa actgaaaaaa ctgccattca aaggcctgat actggacacg gtttttaccg
     1201 aaactcattt gcgtgccgtt gtaaaactgc gcttgtatat taaacgaccc aaccattaaa
     1261 aacagcaaag caatgcgcaa acgtaacata taggctaaca acgtaaattc agtatgatta
     1321 tggcga
//
LOCUS       DAOMAA010000757         1323 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_126102_length_1323_cov_3.379338, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000757 DAOMAA010000000
VERSION     DAOMAA010000757.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126102_length_1323_cov_3.379338"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1194
                     /locus_tag="PLO59_09485"
     CDS             <1..1194
                     /locus_tag="PLO59_09485"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HQV01379.1"
                     /translation="ITGTFDLINMKIVLESNKGTSINLPLSTPEEVGQSGFITFIKHD
                     TIKTNPIKVNNDLLGMNIDLELAITNDAEIRLIFDSKIGDVINGIGNGDLRITVSNDG
                     TFNLFGNYIIDKGSYYFTMQNLFGKYFQLIKGGSVKWIGDPYQGIVDMQARYTRNAGL
                     YDLLQDTSAQYKRKVPVEVSLLLKNQLLNPDISFKINVPDVDANTANLIQRYLNSEEE
                     INKQAMSLIVFNKFATPSLLEGSSTFSNSANVGASLSEFVTQQLSNWASQINSNFNVD
                     FSYTPGDVVTNEQYDIAVSTKLLDDRVKLTGTVGYGNNNNSSSSSSNNTTNVVGDFLV
                     EVDVSKDGRFKVRAFNKSNNNSLINSSISPYTQGVGWFYRKEFDKFSDLFKAKPKATD
                     KNVIK"
BASE COUNT          470 a          221 c          248 g          384 t
ORIGIN      
        1 attacaggta catttgacct tattaatatg aaaattgttt tagagtcaaa taaaggcaca
       61 agtattaatc taccgctatc aacacctgaa gaagttggcc aaagcgggtt tattactttt
      121 atcaaacatg atacgataaa aacaaatcca attaaagtaa ataatgactt attaggaatg
      181 aatattgact tggaactggc tataaccaat gatgcagaga taagattgat attcgactcg
      241 aaaataggcg atgtaataaa tggtattggt aacggtgatt tacgtattac ggtaagtaat
      301 gatggaacct ttaatttatt tggaaattac ataattgaca aaggcagtta ttactttacc
      361 atgcaaaacc tgtttggaaa atattttcag ctgataaaag gtggttcggt taaatggatt
      421 ggcgatccat accaaggtat tgttgatatg caggcgcgct atacccgcaa tgcaggatta
      481 tatgatttac ttcaagatac cagtgcacag tacaaacgaa aagtacctgt agaggtttca
      541 ttacttttaa aaaatcaatt gcttaacccc gatatttctt tcaaaataaa tgtgcctgat
      601 gttgatgcaa acactgccaa cctgatacaa cgatatttga acagcgaaga agaaattaat
      661 aagcaggcca tgagcttaat tgtgtttaac aagtttgcta cgccatcgct attagaagga
      721 agtagcacat tttctaattc tgcaaatgtg ggtgcaagcc ttagcgaatt tgttacgcaa
      781 caattgagca attgggcttc tcaaataaac agtaatttta atgttgattt ttcatacaca
      841 cccggagatg tggttaccaa tgaacaatac gatattgcag taagcaccaa attgcttgac
      901 gacagggtga aacttacagg tacggtaggt tatggcaata acaataacag cagtagcagc
      961 agtagcaaca atacaaccaa tgtagttggc gattttttag ttgaagtaga tgtaagtaaa
     1021 gatggtcgtt ttaaagtaag agccttcaat aaatcaaaca acaactcatt aataaactca
     1081 tccatatcgc catatacaca aggtgtgggt tggttttatc ggaaagagtt tgataagttt
     1141 agcgacctat ttaaggcaaa acctaaagca accgacaaaa atgttattaa ataagatgat
     1201 gcaacttcat gctgctataa actatttgcg ttgcaaaaac agcatactga ttatgctgct
     1261 gttttgtact gcttgcaaaa ccacacaacc ggtagttcaa tcaccacagc aacatattcc
     1321 cat
//
LOCUS       DAOMAA010000758         1322 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_126283_length_1322_cov_3.365430, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000758 DAOMAA010000000
VERSION     DAOMAA010000758.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126283_length_1322_cov_3.365430"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="PLO59_09490"
     CDS             <1..457
                     /locus_tag="PLO59_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01380.1"
                     /translation="DGNNIADSKKRIGLTGGVYPVSNVDFSQVTQTIYEAMTNGDGGD
                     IPENNIEAVLTACQKFSDSEIVMIADNFATPRDIELTNKLCRPIHIVLCGTYGGINTN
                     YMDIARSNKGTLHTIDSDIINLSQITEGQELIIDGHIYQLKNNKFEMIK"
     gene            510..>1322
                     /locus_tag="PLO59_09495"
     CDS             510..>1322
                     /locus_tag="PLO59_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008627615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HQV01381.1"
                     /translation="MEFEITAEFNPTGDQPEAIRQLVAGLQSGERSQVLLGVTGSGKT
                     FTIANVIQQIKRPTLILSHNKTLAAQLYGEFKHFFPKNAVEYFVSYYDYYQPEAYIPS
                     SNTYIEKDLSINAEIEKLRLSTTSTLLSGRKDVIVVSSVSCIYGMGNPADFTENIIRL
                     QLQQSISRNTFLHKLVTSLYARTTDDFKRGTFRVKGDTVDVFLAYADFAYRISFFGDE
                     IDALQTIDPVSGRLVSNHDFIAIFPANIFVTSAKTQQQAIYNIQEDMMKQVNF"
BASE COUNT          438 a          233 c          256 g          395 t
ORIGIN      
        1 tgatggaaat aatatagccg atagtaagaa acggattggg ctaacagggg gtgtgtaccc
       61 ggtaagcaac gttgatttta gtcaggttac acaaaccatt tacgaagcaa tgacgaatgg
      121 cgatggaggc gacatccctg aaaataatat tgaagctgta ttaacagctt gtcaaaaatt
      181 ttcagattca gaaattgtga tgatagccga taattttgct acaccacgcg atattgaact
      241 aactaataaa ttatgcaggc ccatacatat tgtactttgt ggtacttatg gtggcattaa
      301 taccaattac atggatattg caaggtcaaa taaaggcacc ttgcatacta ttgacagcga
      361 tattattaat cttagtcaga ttaccgaagg gcaggagcta attattgatg gacatatcta
      421 tcaactaaaa aacaacaaat ttgaaatgat aaagtagatt ttgcttcgcc caaaaagtaa
      481 acacttactt tgccttacat caaactaata tggaatttga aataactgct gagtttaatc
      541 caacaggcga tcagccggaa gcaatcaggc aattggtagc gggtttacaa agtggcgaaa
      601 gatcgcaggt gctattaggc gttaccggct cgggcaaaac gtttaccatt gccaatgtta
      661 ttcaacagat aaaacgaccc acacttatat tgtcgcacaa taaaacctta gctgcacaac
      721 tgtatggtga gtttaaacac ttttttccaa aaaatgcagt cgagtatttt gtttcctact
      781 acgattacta tcagcccgaa gcatatatac ccagtagcaa tacatacatc gaaaaagatt
      841 tatcaataaa tgccgaaatt gaaaaactgc gtttaagtac taccagcact ttattaagtg
      901 gccgtaaaga tgttatagtg gtatcaagtg tatcatgtat ttatggtatg ggtaaccctg
      961 ccgattttac cgagaacatt atccgtttac aattacagca aagtatttcg cgcaatacgt
     1021 ttttacataa attagttacc agtttgtatg cacgcactac agatgatttt aagcgtggca
     1081 cgtttagagt taaaggcgat accgttgatg tatttttagc ttatgccgat tttgcttatc
     1141 gcatttcatt ttttggtgat gaaattgatg cattgcaaac tattgatccg gttagcggtc
     1201 gcttagtttc aaaccatgat tttatagcca tatttccggc aaacattttt gttaccagtg
     1261 caaaaacaca gcagcaggca atttataata tacaggaaga tatgatgaag caggttaatt
     1321 tt
//
LOCUS       DAOMAA010000759         1321 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_126518_length_1321_cov_2.777251, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000759 DAOMAA010000000
VERSION     DAOMAA010000759.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126518_length_1321_cov_2.777251"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1321)
                     /gene="pruA"
                     /locus_tag="PLO59_09500"
     CDS             complement(<1..>1321)
                     /gene="pruA"
                     /locus_tag="PLO59_09500"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HQV01382.1"
                     /translation="NGEEVRSNKKKKLAPPHDHQHVLGYYHEGDGTHVTQAINAALQA
                     RTAWAAMEWEQRAAIFLKAAELLAGPYRPIINAATMLGQSKNAYQAEIDSACEMIDFL
                     RFNVSYAREIYTQQPISSKGVWNRVEWRPLEGFVFALTPFNFTAIAGNLPTCVAMMGN
                     TVVWKCSNTQIYAARVLMEVFIKAGLPAGVINLIYVPGGVAGDIIFSHRDFAGIHFTG
                     STGVFQNIWKTIGNNIHRYKSYPRIVGETGGKDFILMHESASSSEVATALARGAFEYQ
                     GQKCSAASRAYIPSSKWDEVKAHLIADLATFKMGGTEDFGNFVNAVIDEASFDKLSQY
                     IDNAKQSNEVEIIAGGTYDKNKGYFIAPTVLLAKKPDYITMCEELFGPVLTIYVYDES
                     NFDAALDLVDTTSEYALTGSIMATDRYIIEKTTKRLVHAAGNFYINDK"
BASE COUNT          382 a          288 c          245 g          406 t
ORIGIN      
        1 tttatcgtta atgtaaaaat tgcctgctgc atgcaccaac cgtttcgttg ttttttcaat
       61 aatatatctg tcagtagcca ttatcgatcc ggttaaagcg tattcgctgg tagtgtcaac
      121 caaatctaaa gccgcatcaa aattgctttc atcataaaca taaatggtta aaacaggtcc
      181 aaatagctct tcacacatgg ttatatagtc cggttttttt gctaataaaa cagttggcgc
      241 aataaaatat cctttgttct tatcataagt accaccggct ataatttcaa cttcattgct
      301 ttgctttgca ttatcaatgt attgcgataa tttatcaaag cttgcttcat caattactgc
      361 attaacaaag ttgccaaaat cttcggtacc gcccatttta aaggtagcta agtcagcaat
      421 taaatgtgct ttaacctcgt cccatttgct tgatggaatg tatgcccgcg atgctgccga
      481 acatttttga ccttgatact caaatgcacc gcgtgccaaa gctgttgcta cttcggacga
      541 tgaagccgat tcatgcatca aaataaaatc cttaccaccg gtttcgccta caatgcgtgg
      601 atacgattta tagcgatgta tgttattacc aatggttttc cagatatttt gaaatacgcc
      661 tgttgagcct gtaaaatgga tacctgcaaa atcgcgatgc gaaaaaatga tatcacctgc
      721 aacaccaccc ggaacgtaaa tgaggttaat tacaccggct ggcaaacctg ctttaataaa
      781 tacttccatt aaaacacgtg ctgcataaat ttgtgtattg ctgcatttcc aaacaacagt
      841 attgcccatc atggcaacgc atgtaggcaa attgcctgca attgctgtaa aattaaatgg
      901 agttaaggca aacacaaatc cttctaaagg cctccactct actctgttcc acacaccttt
      961 tgatgaaata ggttgttggg tataaatttc gcgtgcatac gaaacattaa accttaaaaa
     1021 gtcaatcatt tcgcaggccg aatcaatctc agcctgatag gcatttttag attggcctaa
     1081 catggtagct gcattaatta tggggcggta agggccggct aacaactcag cagcttttaa
     1141 aaagatagcc gcacgttgtt cccattccat tgcggcccag gcagtacgtg cttgcaaagc
     1201 cgcattaatg gcttgggtta catgtgtgcc atcgccttca tgataatagc ctaatacatg
     1261 ttgatggtca tgtgggggtg ccagtttctt tttcttgtta gaacgtactt cttcaccgtt
     1321 t
//
LOCUS       DAOMAA010000760         1319 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_126856_length_1319_cov_3.037975, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000760 DAOMAA010000000
VERSION     DAOMAA010000760.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126856_length_1319_cov_3.037975"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            262..546
                     /locus_tag="PLO59_09505"
     CDS             262..546
                     /locus_tag="PLO59_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01383.1"
                     /translation="MKKLLLTIAILISAINSHAWVIQYANVTYATHPNCADGSITVQV
                     WGTSIDIFIISVNGPNGTLQECKASDNFGSSYFTHTFNGLYPGAWHVEVW"
     gene            604..>1319
                     /locus_tag="PLO59_09510"
     CDS             604..>1319
                     /locus_tag="PLO59_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01384.1"
                     /translation="MGFNNTCTPPYIVNQTPATANCNGSITFSDGQVISNLPAGPYTH
                     NGLYNCGCSPVGFSVSTTIDYLPCALTFTPAVTPSDFGCNNGTVSFTLSQAPYFNYYD
                     VTIDGVNSNYYASHTNQTNAYQMFDNLPSGDYEIAVYEHTTLGQNCYCTNLITVTIPE
                     NVCDSITLVGQTPANGNCGGSVTLSNGQTFFNLPAGPFNHIISYTCGCDTIEYTINTT
                     ITTETCFTVTVTAYDMTAPQT"
BASE COUNT          422 a          276 c          218 g          403 t
ORIGIN      
        1 tatccggctt aagttctgat tgaagtcgca tcgcttaata atatctgcat tttaatttaa
       61 tgcaccccat ttttttttga aaacaaatgc aaaacctgcc aaaaattttg agcaggtttt
      121 ttttcagtaa accatttatc accaaaatac aaccgcataa tctttcaaat tagcttggca
      181 ttcattagtg caatacatca tataacctaa ctatagtttt ggtttatcaa tacaatactt
      241 aaaccttaaa aaattacagt tatgaaaaag ctacttctta caatcgcaat cctaataagt
      301 gcaattaact cgcatgcctg ggtaatacaa tatgcaaacg ttacttatgc aacccatcca
      361 aattgtgccg atggatcaat tacggtacag gtatggggta cttcaataga tatatttata
      421 attagtgtaa acggcccaaa tggtactttg caagaatgta aggcttctga taattttggt
      481 tcgtcttact ttacacatac atttaatggc ctataccccg gtgcatggca tgttgaagta
      541 tggtaagccg gcccattatg tgcatacgtc ctacccggtc aatacagaga tgtttggtgt
      601 aatgtgggct ttaacaatac ttgcacgcct ccatacattg ttaatcaaac acctgcaaca
      661 gcaaattgta atggctctat tacttttagc gatggtcagg taattagcaa cctacctgcc
      721 gggccataca cccataatgg tttatataac tgtggttgta gtccagtggg ttttagtgta
      781 agcaccacta ttgattattt accctgcgca ctcactttta cacccgcagt tacgccatca
      841 gattttggct gtaacaatgg cacagtttcg tttacacttt cacaagcacc atattttaat
      901 tactatgatg taactataga tggcgtaaac agcaattatt acgcaagcca tacaaatcaa
      961 actaatgctt atcaaatgtt tgataaccta ccatcgggcg attatgaaat agctgtttat
     1021 gaacatacaa cactgggcca aaattgttac tgtaccaatt tgataacagt aaccataccc
     1081 gaaaacgtat gcgattcgat tacgttagtt ggccaaacgc ctgctaatgg aaactgtggt
     1141 ggatcggtta cattaagtaa cggacaaaca ttttttaatc ttccggcagg gccatttaac
     1201 catattatca gttatacctg tggatgcgac actattgaat atacaataaa cacaaccatt
     1261 acaactgaaa cctgctttac agtaaccgta accgcatacg atatgacagc accacaaac
//
LOCUS       DAOMAA010000761         1317 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_127255_length_1317_cov_2.916006, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000761 DAOMAA010000000
VERSION     DAOMAA010000761.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127255_length_1317_cov_2.916006"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="PLO59_09515"
     CDS             complement(<1..555)
                     /locus_tag="PLO59_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQV01385.1"
                     /translation="MSSLIGYIAIGVFITGIGTFMWLVPTESAGSNVLENGFANIDPL
                     FILAPWVYLFLIPAITMRSFSEEKKTGTIELLLTRPLTELQIVLAKYFAGVVLVIVSL
                     LPTLIYYYSVHQLGAPKGNIDTGGMWGSYIGLLFLGAGFVAVGTFASAISENQVIAFI
                     MALLLCFITYIGFDFISASGIFGKY"
     gene            944..1315
                     /locus_tag="PLO59_09520"
     CDS             944..1315
                     /locus_tag="PLO59_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01386.1"
                     /translation="MTSLCYFGLFYMIYDFNIWPAIKLFILGGGLCIFFAAIVTLWWQ
                     ISAHMVGIGGLIGALLAICYFMQMPILLAICACVMLAGCIGFARLKLNAHTPTQVYVG
                     FIFGCLLQFSLFFLAQSITFV"
BASE COUNT          419 a          223 c          203 g          472 t
ORIGIN      
        1 gtatttgcca aatatgcctg aagctgaaat gaaatcgaag ccgatataag taataaaaca
       61 taatagtaaa gccataataa aggcaatcac ttgattttcg gaaatagcag atgcaaaagt
      121 accaaccgct acaaagcccg cacctaaaaa caataaacca atatacgagc cccacatgcc
      181 accagtatca atattacctt ttggcgcacc taattgatgt accgaataat aatatattaa
      241 agttggaagt aatgatacaa ttaccaaaac tacgccagca aaatattttg ctaatacaat
      301 ttgtaattct gttaaaggtc gcgttaatag taattcaatg gttccagttt tcttttcttc
      361 cgaaaagcta cgcatggtaa ttgctggaat taagaacaaa tacacccaag gcgctaaaat
      421 aaataagggg tcaatattgg caaagccatt ttctaaaaca tttgagccag cactttcggt
      481 tggcactaac cacataaaag taccaatgcc tgtgataaaa actcctatag caatatagcc
      541 aatcagtgag ctcaaaaaac ttcgtatttc tttaattaat aaggtaaaca tacaaaaaca
      601 aaaatagcaa tagattttga aaatatacta ttatcttata ctaactcgat tttttttaat
      661 gtaatttagc attctcttaa acatgattaa aacggctaat attatttctc atctgtttca
      721 tccattatta atggctactt acggttgttt aattgttttt tttggcttaa cagataccat
      781 ttattttatc ttcacccctt ttaaattaaa gctggtttta accttcacta ttttttcttt
      841 taccttttta ttgccagttt taaatttgct gattttatat aaattaggat atgtttcttc
      901 tttaaggatt gaaaatagaa gagaaagagc ctttccatta ttaatgactt ccttatgcta
      961 ttttggattg ttttatatga tttatgattt caatatttgg ccggctatta aactatttat
     1021 tttaggcgga ggcttatgca ttttttttgc agcaattgtt actttatggt ggcaaattag
     1081 cgcacacatg gtaggcattg gcggcttaat tggtgcttta ttagctattt gttatttcat
     1141 gcagatgcct atattattag ccatttgcgc ttgcgttatg cttgcaggtt gtattggttt
     1201 tgctcgattg aagcttaatg cacacacgcc tacacaagta tatgtaggct ttatttttgg
     1261 ctgtttgctg caattttctt tatttttctt ggcacaaagc attacatttg tttagtc
//
LOCUS       DAOMAA010000762         1316 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_127502_length_1316_cov_2.417922, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000762 DAOMAA010000000
VERSION     DAOMAA010000762.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127502_length_1316_cov_2.417922"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="PLO59_09525"
     CDS             <1..499
                     /locus_tag="PLO59_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HQV01387.1"
                     /translation="ADVSEIKKPIEMQLPVALPTLAQSNPHLSGHYIFAQIQTVLQPA
                     LPLETFHIEKLKQAFQPKSTKAKTDVFEYSSMLEVDLHIEAILPDKQVAAAETLQIQL
                     QHFRQALDNAIVQRRKSLTFIHGIGNGILKQKIIAELNDYKGIVHKPASIQKYGLGAI
                     EVILK"
     gene            501..>1316
                     /locus_tag="PLO59_09530"
     CDS             501..>1316
                     /locus_tag="PLO59_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS C-terminal target domain-containing
                     protein"
                     /protein_id="HQV01388.1"
                     /translation="MKLIVFGVLLFGLNSAIIAQTGCTDPQAINYNVSAIINDGSCMY
                     ANTTLNTGIIAPLQTPLLDECSGLIATNGNVWMHVDDTDNRLFKMDVVTGDTLQSTAL
                     QATNMDWEDMDADSNSIYIGDIGNNSGNRTNLQFYKALISQLNMPLVSTQSITYSYAD
                     QVNFNPALNNTPWDAEAFFILNDSIHLFTKDWVTKYTKHYILPTLPGNYAATLVDSFN
                     VGGLITSAAIDKAGTIVLLGYDNTIAAPCFLWMLYDYQPGKIFSGNKRKFNLGS"
BASE COUNT          439 a          236 c          241 g          400 t
ORIGIN      
        1 agccgatgtt tccgaaatta aaaaacctat tgaaatgcaa ttgcccgttg cattaccaac
       61 tttggcacaa agtaatccac atttaagtgg tcattacata tttgcacaaa tacaaactgt
      121 tttacaacct gcacttccgt tagaaacatt tcatatagaa aaattaaaac aggcttttca
      181 acctaaaagt acaaaagcaa aaaccgatgt ttttgaatat agtagcatgc ttgaggttga
      241 cttgcatatt gaagccatat tacccgataa gcaagtagct gcggccgaaa ctttgcaaat
      301 tcaattacaa catttcaggc aggctttaga taatgccatt gtgcaacgta gaaagtccct
      361 aacttttatt catggtattg gcaatggtat tttaaaacaa aaaattattg ccgagctaaa
      421 cgattacaaa ggtattgtgc acaagccggc ctctatacaa aaatatggct taggtgccat
      481 cgaagttata ttaaaataaa atgaaactaa tagtttttgg agtcttgtta tttggactaa
      541 actcagccat tattgcccaa acaggatgta ccgacccaca agcaatcaac tataatgtat
      601 cggctataat taatgatggc agttgtatgt atgctaacac cacacttaac accggcatta
      661 ttgcaccatt gcaaactccg ttgttagatg aatgttcggg tttgattgct accaatggta
      721 atgtatggat gcatgtggat gatacagaca atcggttatt taaaatggat gttgtaaccg
      781 gtgatacctt gcaaagtaca gcactgcaag ctacaaatat ggattgggaa gatatggatg
      841 ccgacagcaa ttcaatatac attggcgata ttggtaacaa tagtggcaac cgaaccaatt
      901 tgcagtttta taaagcttta ataagccaac taaatatgcc attggttagt acacaaagca
      961 ttacttacag ttatgccgat caggttaatt ttaatccggc attaaataat acaccctggg
     1021 atgctgaagc attttttatt ttaaacgaca gcatacatct gtttaccaaa gattgggtaa
     1081 caaaatatac gaaacattat atactgccaa ccttgcccgg taattatgct gctactttag
     1141 ttgatagttt taatgttggc ggactgataa cttcggctgc tattgataaa gcaggtacca
     1201 ttgttttatt aggttatgat aacaccattg cagcaccttg ttttttgtgg atgctttacg
     1261 attaccaacc cggaaaaatt tttagcggta ataagcgcaa gtttaattta ggtagt
//
LOCUS       DAOMAA010000763         1312 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_128313_length_1312_cov_2.333333, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000763 DAOMAA010000000
VERSION     DAOMAA010000763.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128313_length_1312_cov_2.333333"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1312
                     /locus_tag="PLO59_09535"
     CDS             <1..>1312
                     /locus_tag="PLO59_09535"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01389.1"
                     /translation="TSRFRFDVIGRADVFTDVYASRLVPNTYYVCGFSRSTTDMYCIV
                     MSVSSTGVVNWFNTYWLDPTASATSPFNSHCRANALVEDINTGNIVVVGSIMDPNSTI
                     TGRDGLVFTLNAAGGLLCATSHHVFIDDSYEDVIQKSNGMFAAAGYVGDGTTVPATFY
                     GIWLTELTPFCGPMATARYLQGPFTATNPGQCKGFSIIERQNTGGAFEYYIAGPNKAI
                     GGDIATITKINAVPGLPPVAWYGYMGQSGAFESGYAVDYSQAANTKPGLVLFTNTNAP
                     AATFGANDSYMMKSYFNGATCIFNCNPQPPVVQAIPLAIANCSDSVNNIYKRNALVAL
                     NSPYTFGVICTQNNIACGSNAKLTDLAEEVMLDIYPNPASDLANLQLFVENEAQYIIE
                     AVDITGRTTLLSDSYLPAGEQPVPLDISNLSKVVYSILLRGNGKV"
BASE COUNT          365 a          267 c          279 g          401 t
ORIGIN      
        1 cacatcacgt tttcgttttg atgttatcgg tcgtgccgat gtgtttacag atgtatatgc
       61 cagccggtta gtgccaaata catattatgt atgtggcttt tccagatcaa ctactgatat
      121 gtattgcatc gttatgtcag tttcgagtac aggtgtagtt aactggttta atacctattg
      181 gttagatcct accgcttcgg ctacaagccc gtttaactca cattgccgtg ccaatgcatt
      241 ggttgaagac ataaacaccg gtaacattgt tgtagttgga agcattatgg atcctaactc
      301 taccataacc gggcgcgatg gtttggtgtt tacattaaat gctgctggtg gtttgttatg
      361 cgctacatca catcatgtat ttattgacga ttcgtatgaa gacgttattc aaaaaagcaa
      421 tggcatgttt gctgctgctg gttatgtggg tgatggtacc acagtacctg ccacatttta
      481 tggaatttgg cttactgaat taaccccatt ttgtggccca atggctacag cgcgttattt
      541 acaagggcct tttacagcaa ccaatccggg tcagtgtaaa gggttttcaa ttatcgaacg
      601 tcaaaacacc ggaggtgcat tcgaatatta tatagccgga ccaaacaaag ccattggtgg
      661 agatattgcg acaataacaa aaattaatgc cgtgcccggt ttaccacctg tggcttggta
      721 tggttatatg ggacaaagtg gtgctttcga aagtggctat gccgttgatt attcgcaagc
      781 tgcaaacacc aaacccggtt tggtgttgtt taccaatacc aatgctcctg cagcaacttt
      841 tggcgcaaac gattcataca tgatgaaatc atattttaat ggtgcaacct gtatattcaa
      901 ttgcaatccg caaccgcccg tagttcaagc tattccatta gcaattgcaa attgcagcga
      961 ttcagttaac aacatctata aacgtaatgc cctggttgca ttaaactcgc catacacctt
     1021 tggcgttata tgcacacaaa ataatattgc ttgcggcagc aatgccaaac taactgattt
     1081 ggccgaagaa gtaatgcttg atatttatcc aaaccctgcc agcgatttgg ctaatttgca
     1141 attgtttgtt gaaaacgaag cgcagtatat tatcgaagca gtggatatta caggccgcac
     1201 aaccttatta tcagatagtt atttgcctgc aggcgaacaa cctgttccat tggatatttc
     1261 aaacttatca aaggtggtgt atagtatttt actacgtggc aacggaaaag ta
//
LOCUS       DAOMAA010000764         1311 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_128532_length_1311_cov_2.277866, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000764 DAOMAA010000000
VERSION     DAOMAA010000764.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128532_length_1311_cov_2.277866"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1311
                     /locus_tag="PLO59_09540"
     CDS             <1..>1311
                     /locus_tag="PLO59_09540"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HQV01390.1"
                     /translation="QHLQTQLDNDPAMRLRMEQIEQQTQDYLLQKNSNSTQAVVTIPV
                     VFHVLYNVNNATQNVSDARIMEQLNVINLDFSHTNADAGNAPAPFLAVAANTNIQFCL
                     AQRDPNGAATTGIIRKQTSVTSFTQNNNVKFNSTGGADAWPVASYMNVWVCQLGSGLL
                     GYAQFPGGTASTDGIVVLNGSVGGPAAPGTSSPYHLGRTLTHEVGHWLNLRHIWGDAN
                     CGNDLVNDTPTQQTSNGGCPSYPHVTCSNGPNGDMFNNYMDYVNDNCMNMFTAGQSAR
                     SNALFAPGGARVSLTTSQGCVPVGGGCGKPTGLAASGITSSGATLTWAAVSGATSYNF
                     RYRLSPSGSWVNSTVTTNSKTLTGLSANTLYDYRVRAVCSVTGQYSAIAQFTTLSGGG
                     CSDNYEPNNGKSVAKPIAINTNINAIISSATDKDWLTFTTTNAQP"
BASE COUNT          384 a          298 c          275 g          354 t
ORIGIN      
        1 cagcatttac aaacccagtt agataacgac cctgccatgc gactacgtat ggagcaaatt
       61 gagcaacaaa ctcaagatta tttattacaa aaaaacagca acagtacaca agcggttgta
      121 acgatacctg tagtttttca cgtattgtac aatgttaaca atgcaactca aaatgtatct
      181 gatgctcgca ttatggagca attaaatgtg atcaatctcg attttagtca taccaatgcc
      241 gatgcaggta atgcacctgc tccattttta gccgtagctg ctaacaccaa tattcagttt
      301 tgcttagcgc aacgcgatcc taatggagct gcaacaacgg gtattatccg taaacaaact
      361 tcggtaacaa gtttcacgca aaacaataac gtaaagttta actcaaccgg tggtgctgat
      421 gcttggcctg ttgctagtta tatgaacgtt tgggtatgcc agttaggaag cggcttatta
      481 ggttatgcac aattccccgg aggtactgca agtacggatg gtattgtagt tttaaacggc
      541 tcagttggtg gccctgctgc acccggtaca tcatcacctt accacttagg ccgtacacta
      601 acgcatgaag taggtcactg gctaaacctg cgtcatattt ggggtgatgc caattgcggt
      661 aacgatttgg taaatgatac gcctacacaa caaacatcta acggtggctg tccttcatat
      721 ccacatgtaa cttgcagcaa tggtcctaat ggcgatatgt ttaacaacta tatggattat
      781 gtaaacgata attgtatgaa tatgtttaca gcaggccaat cggcacgtag caatgcctta
      841 tttgctccgg gcggtgcacg cgttagctta actacttcac aaggttgtgt acctgtaggt
      901 ggtggctgtg gcaaaccaac cggcttagct gcatcaggca ttacttcgtc aggcgctaca
      961 ttaacctggg cagcagtaag cggtgcaacc agttataatt tccgttaccg cttaagccca
     1021 agcggttcgt gggtaaacag tacagttacc accaattcga aaacacttac aggtttatct
     1081 gctaacacac tttacgacta tcgtgtgcgt gccgtttgct ctgtaacagg acaatactct
     1141 gcaatagctc aatttactac tttatcgggt ggcggatgca gcgataatta tgagccaaat
     1201 aatggcaagt cggttgcaaa accaatagcc atcaatacca atatcaacgc aattatcagc
     1261 tcagctaccg ataaggactg gttaactttt acaaccacca atgcacaacc t
//
LOCUS       DAOMAA010000765         1311 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_128578_length_1311_cov_1.889331, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000765 DAOMAA010000000
VERSION     DAOMAA010000765.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128578_length_1311_cov_1.889331"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="PLO59_09545"
     CDS             <1..519
                     /locus_tag="PLO59_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HQV01391.1"
                     /translation="ISAGNFHGQPLALVLDFLAIALAELGNISERRTYLLISGQRGLP
                     SFLVANAGLNSGFMIPQYTAASIVSQNKQYCTPASVDSIVSSNGQEDHVSMGANAATK
                     CWHVVNNVQTILAIEMLNAAQALHFRRPLKSSPMLEAFFSDYRKQVSYYDNDRVLFED
                     IEKSHIFLFGKK"
     gene            complement(628..>1311)
                     /locus_tag="PLO59_09550"
     CDS             complement(628..>1311)
                     /locus_tag="PLO59_09550"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HQV01392.1"
                     /translation="SDSVFFRSMDRDTGAVVKLSKPTCKGCIEEGMMLMSAGDSATFI
                     ASADSFFLKTIGQDLPPFVPKGSKIKFEVKLHSIMTEAQRSNMLKEAETKSLAAYIQK
                     NNITATPTASGLIYIETTAGTGVTPADGDSISVHYVGKFLDGRVFDESTGKDPITFPL
                     GQQMVIPGWEEAFKLMKSGGKATLITPSAVAYGERGYAPVIPASTTLVFDVELVKVVK
                     AAGATTSAK"
BASE COUNT          376 a          281 c          249 g          405 t
ORIGIN      
        1 atcagtgccg gtaattttca tggacaacca ctggcattgg tgttagattt tttagcaatt
       61 gccttagccg aattaggcaa catatccgag cgcagaacct atttgctgat atccggccaa
      121 cgtggtttac catcgttctt agtagccaat gcaggactta acagtggctt tatgattcca
      181 cagtacacgg ctgcttcaat cgtatcgcaa aacaagcagt attgtacacc ggcatctgtt
      241 gattcaattg tttcgtcaaa cggacaagag gaccatgtaa gtatgggtgc caatgcagct
      301 acaaaatgct ggcatgtggt taacaatgta caaaccattt tagcaatcga aatgcttaat
      361 gctgcacaag ccttgcattt cagacgtcct ttaaagtctt cgccaatgct cgaagcgttt
      421 tttagcgatt accgcaaaca agtaagttac tatgataacg acagggtttt atttgaagac
      481 attgaaaaaa gccatatctt tttatttggt aaaaaatagc aaactggcag gttctatttt
      541 gcctgataca tatcgtattt aatcaaaact atttcgcata aaaaaaggag gctgtgcctc
      601 cttttttact tgaatgctaa tcagaaatta ttttgccgaa gttgttgcgc ctgcagcttt
      661 aaccactttt accaactcaa catcaaatac caatgtagtg cttgccggta ttacaggcgc
      721 ataaccacgc tcgccataag caacagccga tggtgtgatt aaagtagctt tgccaccaga
      781 tttcattaat ttaaatgctt cttcccagcc cggaattacc atttgctggc ctaacggaaa
      841 tgtgatgggg tcttttccgg ttgattcgtc aaatacgcgg ccatctaaaa acttacctac
      901 ataatgtacc gatatcgaat cgccatcagc aggtgtaact ccagtaccgg cagtagtttc
      961 aatataaatt aagccacttg ctgttggtgt tgccgtaatg ttgttttttt gaatataagc
     1021 agccaacgat tttgtttcgg cttctttaag catattgctt ctttgggctt cagtcatgat
     1081 gctatgtaac ttaacttcaa attttatttt gcttccctta ggtacaaaag gtggtaaatc
     1141 ctgaccaata gtttttaaga aaaacgaatc ggcactggct ataaatgttg cactatcacc
     1201 tgcactcatc aacatcatac cttcttcgat acaacctttg catgtaggtt tgcttagttt
     1261 aactacagca cctgtatctc tgtccataga gcggaagaaa accgaatcgc t
//
LOCUS       DAOMAA010000766         1308 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_129071_length_1308_cov_2.657622, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000766 DAOMAA010000000
VERSION     DAOMAA010000766.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129071_length_1308_cov_2.657622"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="PLO59_09555"
     CDS             complement(<1..608)
                     /locus_tag="PLO59_09555"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF020140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HQV01393.1"
                     /translation="MSNYYQPSNTVMPPVIKGIIVLNVIMYLITMASEYFGFHQQLFN
                     LFALHYYGSELFQPHQFVTYGFLHGGLGHIFFNMFALWMFGAMLENYWGSKRFFIYFI
                     FTVVGAGVTQMISQHFEISALSKHVSAFINNPDPMDFRVLFEQNMDGMGAEFRSFGAQ
                     LASTYETQPHNPEVIDNAIMFAQNLIPYQASTPLVGASGGVYG"
     gene            763..>1308
                     /locus_tag="PLO59_09560"
     CDS             763..>1308
                     /locus_tag="PLO59_09560"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis acyltransferase"
                     /protein_id="HQV01394.1"
                     /translation="MQIISYVIYYGFVKPLSMLPMFILYGISDVLYIILWFVIPYRKK
                     VVLQNLQRSFPEKSNAECLKIAKQFYRHFTDVMVETLKGFSASKQWLNTKVIGHNTDL
                     LEKYFLQNKSIIAITGHYGNWEWGALTFSEHTKHKPYGIYAPLNNRFWNNVLINSRKK
                     FGTTLIAVKEVGQVFTANLNEL"
BASE COUNT          436 a          221 c          251 g          400 t
ORIGIN      
        1 ccgtaaacac cacccgaagc gcctaccagt ggtgtgcttg cctgataggg aattaaattt
       61 tgtgcaaaca taatagcatt gtcaattact tcggggttat gtggttgtgt ttcgtatgtt
      121 gatgcaagtt gcgcaccgaa acttctgaac tcagcaccca tgccatccat gttttgttcg
      181 aataaaaccc taaagtccat tgggtcaggg ttattaataa atgcagaaac atgtttgctt
      241 aaagcactta tttcaaagtg ttgcgatatc atttgtgtaa caccggcacc aacaactgta
      301 aaaataaagt agataaaaaa acgtttactg ccccaatagt tttcgagcat agccccaaac
      361 atccataaag caaacatgtt aaaaaatata tgacccaagc caccatgcaa gaagccataa
      421 gtaacaaact gatgtggctg aaaaagttct gaaccatagt agtgcagtgc aaacagatta
      481 aacaattgtt ggtgaaaccc aaaatattca gacgccattg tgattaaata cataatcaca
      541 tttaaaacta taatgccttt gataaccggt ggcattactg tgttcgaagg ttgataataa
      601 ttactcataa ttgggtgtaa atgcgtgaaa gcgtgcttat gttaattgtt ttgcaatagt
      661 atgtttttag tgcaaggcga aatagtttgt caaatatttt taaccaaacg taaatgcaat
      721 gcaaatgttt acagttaatt gtaaataaaa tatgtttgcc ggatgcaaat aataagttat
      781 gtgatttatt atggctttgt aaagccattg tcaatgctac ccatgtttat tttgtatggc
      841 atttctgacg tgttgtacat tattttgtgg tttgtaattc cttaccgtaa aaaagtggtt
      901 ttacaaaatt tgcagaggtc gtttccggaa aaatcaaatg ccgaatgttt gaaaattgcc
      961 aagcaatttt atcggcattt tacggatgtt atggttgaaa ccttaaaagg tttttcggct
     1021 tcgaagcagt ggcttaacac caaggttatt gggcataaca ccgacttatt agaaaaatac
     1081 tttttacaaa ataaaagcat tattgccata acggggcact atggcaattg ggagtggggg
     1141 gcgctaactt ttagcgagca tacaaaacac aaaccctacg gtatttatgc accgttaaat
     1201 aaccggtttt ggaataatgt acttatcaat tcacgaaaaa aatttggaac cactttaatt
     1261 gctgtaaaag aagtagggca ggtatttaca gccaatctaa atgaactt
//
LOCUS       DAOMAA010000767         1306 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_129428_length_1306_cov_3.007194, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000767 DAOMAA010000000
VERSION     DAOMAA010000767.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129428_length_1306_cov_3.007194"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            284..1072
                     /locus_tag="PLO59_09565"
     CDS             284..1072
                     /locus_tag="PLO59_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007650617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQV01395.1"
                     /translation="MNLDLQGRHALVCGSTQGIGKAAALALAKMECNVTLLSRNENVL
                     QQVIKELNTFQGQKHSYIVADYSNPIALKEKVEKHIAEKNVFHILINNTGGPPAGPAV
                     NAKAEEFLTAFNNHLICNHTLMQACIEGMKQANYGRIVNIISTSVKQPIKGLGVSNTV
                     RAAVANWSKTLSAEVARFGITVNNVLPGATITARLTAIIDDKARKTGKTFNDVTREML
                     SEIPAGRFAEPEEIANVIAFLCSPAAGYLNGINVPVDGGRTTCL"
BASE COUNT          403 a          243 c          259 g          401 t
ORIGIN      
        1 gacttttcat tgcttctgta agcgtaccat cgtttaataa agtatctatc tgcacatgca
       61 ttacaacagg tagtttatct tttaatgctg tgccaatggt ttgaattgct ttttttgctt
      121 gctcatcggt tataccggca tcggttgcta cttttttaat taacgtttcc atgttagtat
      181 ttgtaaataa gtttaaaggt gtgtgtaaat ttattttaat gatttttgaa aacaaattat
      241 ttgcagcctt gctgcaacgc tttgtataaa taaaacaaat tatatgaatt tagatttaca
      301 aggacgccat gcattggtgt gtggcagtac acaaggaata ggcaaagctg ccgcattggc
      361 gcttgctaaa atggagtgca atgttacgtt gctgtcgcgt aacgaaaacg ttttacaaca
      421 agtaataaaa gagctgaaca cttttcaggg acaaaagcac agttatatag ttgccgatta
      481 cagcaacccc attgcattaa aagaaaaagt tgaaaaacac attgccgaaa aaaatgtgtt
      541 tcatattctg ataaacaaca ccggtgggcc accagccgga cctgcagtaa atgcaaaagc
      601 cgaagagttt ttaaccgcat ttaataacca ccttatttgc aatcatacat taatgcaagc
      661 ctgtattgaa ggcatgaaac aagcaaatta cggacgcatt gtaaacataa tttcaaccag
      721 tgtaaagcag cccattaaag gcttgggtgt ttcaaatacg gtgcgagcag cagtggctaa
      781 ctggagcaaa accctatcgg ccgaagtagc acgttttggc ataacggtta ataatgtttt
      841 accgggtgca accattacag caagattaac tgctattatt gatgataagg cgcgtaaaac
      901 cggcaaaacg tttaacgatg ttacccgcga aatgctttcg gagatacctg ccggccgctt
      961 tgccgaaccc gaagaaattg ctaacgttat tgcctttctg tgttcgcctg ctgctgggta
     1021 tctaaacggt attaatgtgc ctgttgatgg tggccgtacc acttgcttat agtattggtt
     1081 gatctttttg tttgaagtaa tttttgcatt gcaacttgtt tggtagtgta gcaactattt
     1141 aatgccatag agaaaacata cttttgtttc gaaataaata aacaggctta accacttatg
     1201 gaaaaaattg ccaactatat taacggtgtt tatacacctc cattatcaaa tgcttattta
     1261 gataatataa acccggccat agccgaggta tatagtctca tacctg
//
LOCUS       DAOMAA010000768         1306 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_129436_length_1306_cov_2.884093, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000768 DAOMAA010000000
VERSION     DAOMAA010000768.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129436_length_1306_cov_2.884093"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1125
                     /locus_tag="PLO59_09570"
     CDS             <1..1125
                     /locus_tag="PLO59_09570"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV01396.1"
                     /translation="YTVTVTNGLGCTASATIAVNGVSAPPVTPGAVSGNNTVCALSSS
                     SYSVSPVAGATNYIWTVGSGATIASGQGTNAITVNFANNAVSANVSVIAGNVCGNSNA
                     STLAYAVVSTLPLKPTVLNGPLYGRCKTNNVAYSCTVMPGPVNYVWTVPAGVTIVSGQ
                     GTPNIVVNFTPAFTNVGTISVYTSNGCGNSAAQVRTVSARPQSPVISGPTSACPNQIL
                     TFTATPVAGATSYIWTVVPGSVIQSGQGTTTLVVKWGNVGGQMYCAANNSCGASVRAS
                     KPVSIVCKEGFGGYNFSEVDLFPNPASGSVQLKLDAAKTGWLTIKVADLVGKVCLLEK
                     TEMQVGDNTFVLDIETLAPGIYQVMVVQEGMMRTKKLVVQ"
BASE COUNT          358 a          263 c          311 g          374 t
ORIGIN      
        1 tatacggtaa ctgtaacaaa tggtttaggt tgtaccgcat ctgcaaccat agcggttaat
       61 ggagttagtg caccacctgt tacaccgggt gctgtttcgg gcaacaatac cgtatgcgct
      121 ttaagtagca gcagttacag cgtttcacca gttgctggtg caaccaatta tatttggacc
      181 gttggtagtg gcgcaactat agcttcgggg caaggtacca atgccataac ggtaaatttt
      241 gccaacaatg ctgtaagtgc caatgtaagt gtaattgcag gcaatgtttg cggaaacagc
      301 aatgcatcaa cgttagctta tgcagttgtt tctactttgc cacttaaacc aacggtatta
      361 aacggcccgc tgtacggacg ttgcaaaacc aataatgtgg catacagttg taccgttatg
      421 ccgggccctg taaattatgt gtggactgtg cctgcaggtg ttaccattgt aagcgggcag
      481 ggcacaccca atattgtagt aaactttaca ccggcattta ccaatgtggg taccattagt
      541 gtatatacat ccaacggttg tggcaatagc gcagcacaag tgcgtactgt aagtgcacgc
      601 ccgcaatcgc ctgtaattag tggccccaca tcggcctgcc ccaatcaaat acttacgttt
      661 accgctacac ctgtggccgg agccacaagt tatatttgga cagtagtgcc cggatctgtt
      721 atacaaagcg gacaaggcac cactacctta gttgttaaat ggggcaatgt tggcgggcaa
      781 atgtattgtg ctgctaataa tagttgtggc gcaagcgtgc gcgcaagtaa gccggtttca
      841 attgtttgta aagaaggttt tggtggctat aatttctctg aagttgattt attcccgaat
      901 ccggcttccg gcagcgtaca gcttaaattg gatgcagcca aaaccggatg gttgactata
      961 aaagttgccg acttggtggg taaagtttgt ttgttggaaa aaacagaaat gcaagtgggt
     1021 gataatactt ttgtgctcga tattgaaaca ttggctccgg gtatttatca ggtaatggtg
     1081 gtgcaggaag gcatgatgcg aaccaaaaaa ttggtggtgc aataaccccg cattagttta
     1141 caataagaaa aggctttcta tgaacatacg ttcatagaaa gcctttttta tgattacacc
     1201 atttgatttt ttgaagggac taataatcat caatagttgt aattttttta tagcttgcgt
     1261 attatcgtat gcaacctgaa attgcctctc cctcgcttaa aatagt
//
LOCUS       DAOMAA010000769         1303 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_130136_length_1303_cov_2.188301, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000769 DAOMAA010000000
VERSION     DAOMAA010000769.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130136_length_1303_cov_2.188301"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1303)
                     /locus_tag="PLO59_09575"
     CDS             complement(<1..>1303)
                     /locus_tag="PLO59_09575"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008764112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HQV01397.1"
                     /translation="LNATVFKASFNRPNLYYEVREKRDILKEIIKYIKQNMGKSGIIY
                     CLSRKKTEEVASTLNVNGIKALPYHAGLEAAVRARTQDDFLMERTDVIVATIAFGMGI
                     DKPDVRFVMHHDIPKSLEGYYQETGRAGRDGREGRCIAFYAYKDIEKLEKFMKGKPIA
                     EQEIGKQLLNETVGFAETSVCRRKVLLHYFGEEYTEENCNNCDNCLHPKTKIEAQDEV
                     CTLLETVIAIKEKFNADHTIHVLMGHGEKAVKDHEHDQLDIFGDGADQDQKFWMAVIR
                     QALLAGFLSKDIDNYGLLKVTDKGHEFLKKPHSFMVTKDHEYTDEDDDDDDSEGAGPG
                     GGGGGGADPELYNMLKDLRKKVAKQKNLPPFVIFQDPSLEEMSIQYPITLDELKNITG
                     VGTGKASKYGAPFIDLINKYVEENEIERAVDLVVKSVVNKSV"
BASE COUNT          338 a          285 c          235 g          445 t
ORIGIN      
        1 accgatttgt ttactacact ttttacaacc aaatcaacag cgcgctcaat ttcattttct
       61 tctacatact tattaattaa atcaataaaa ggagcaccgt attttgacgc tttgcccgta
      121 ccaacacctg taatgttttt taactcatcc agggtaattg ggtattgaat tgacatttct
      181 tccaatgacg ggtcttggaa aataacaaat ggcggcagat ttttttgctt ggcaactttt
      241 ttacgtaagt ctttaagcat attataaagc tcggggtcgg caccaccacc gcctccacca
      301 ccgggtccgg caccttcgct atcatcatca tcatcgtctt catcggtata ttcatgatct
      361 ttggtaacca taaatgaatg tggttttttc aaaaactcat gtcccttatc ggtaactttt
      421 aataagccgt agttatcaat atcctttgat aaaaatcctg ctaaaagtgc ttgtctgata
      481 acagccatcc aaaacttttg atcttggtcg gcaccatcgc caaaaatatc cagttgatcg
      541 tgttcgtggt ctttaacagc cttttcgcca tggcccatca gtacatgaat ggtatggtcg
      601 gcattaaatt tttctttaat cgcaataaca gtttctaaca aagtgcatac ttcatcctgt
      661 gcttcgattt tggttttggg gtgcaggcag ttatcgcagt tgttgcaatt ttcttctgtg
      721 tattcttcac caaaataatg taataaaact tttctgcggc ataccgatgt ttcggcaaaa
      781 ccaacggttt cgtttaataa ttgtttacct atttcctgct cggcaatggg tttgcctttc
      841 ataaactttt cgagtttctc aatatccttg taagcataaa aagctataca acgtccttcg
      901 cggccatcgc ggcctgcgcg tccggtttcc tggtagtaac cttcgagcga tttgggaata
      961 tcgtgatgca ttacaaaacg cacatcgggt ttatctatgc ccataccaaa tgcaatggtt
     1021 gcaacaatta catcggtgcg ttccatcaaa aaatcgtctt gtgtacgtgc acgaacagca
     1081 gcttctaaac cggcatggta cggtaaagct tttataccat ttacgttaag cgttgaagct
     1141 acttcctctg ttttcttacg gctaaggcag tatatgattc cgcttttgcc catattttgt
     1201 ttgatgtact taattatctc ttttagtata tcgcgtttct cgcgtacttc atagtatagg
     1261 tttggccggt taaatgatgc tttaaatacg gttgcattta aaa
//
LOCUS       DAOMAA010000770         1302 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_130363_length_1302_cov_1.800321, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000770 DAOMAA010000000
VERSION     DAOMAA010000770.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130363_length_1302_cov_1.800321"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1302
                     /locus_tag="PLO59_09580"
     CDS             <1..>1302
                     /locus_tag="PLO59_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010855346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HQV01398.1"
                     /translation="IFCYDDKMADYLSGTGRADVAALANTVRNYLRGDDEVYANPQQY
                     FDQVIEIDLSTLEPHINGPFTPDLAWPLSKFAAAVRENNWPAKLEVGLIGSCTNSSYE
                     DITRAASLAKQAVDNGIVAKSEYTITPGSEQVRYTVERDGYLQIFEKMGGVVLANACG
                     PCIGQWARHTDDPTRKNSIITSFNRNFAKRNDGNPNTHSFVASPEIVTALAIAGDLTF
                     NPITDTLTNAQGMQVKLAEPQGIELPLKGFEVADAGYQAPALDGSSVQVIVSPTSDRL
                     QLLEPFAAWEGSNLTNLKLLIKAKGKCTTDHISMAGPWLKYRGHLDNISNNMLIGAVN
                     AFNDKTDNVKNQLTGIYGAVPATQRAYKAAGIGSIVVGDENYGEGSSREHAAMEPRFL
                     GVRAVLVKSFARIHETNLKKQGMLALTFANKADYDKIQEDDN"
BASE COUNT          402 a          278 c          286 g          336 t
ORIGIN      
        1 atattctgtt atgatgataa aatggccgat tatttaagtg gtacaggccg tgctgatgta
       61 gcagcattag ccaataccgt acgtaattat ttacgtggcg atgatgaagt atatgccaat
      121 ccacaacaat acttcgatca ggttatagaa attgatttat caacattaga gccacatatt
      181 aatggtccgt ttacgcctga tttagcttgg cctttaagta aatttgccgc agcggtacga
      241 gaaaataact ggccggcaaa actcgaagtg ggcttaattg gcagttgcac caattcatca
      301 tacgaagata taacccgtgc tgcttcatta gccaaacaag ctgttgataa tggtattgta
      361 gcaaaaagcg aatacaccat tacacccggc agcgagcaag tacgctatac cgttgaacgc
      421 gatggttacc tgcaaatatt tgaaaaaatg ggtggagtgg ttttggccaa tgcctgcggc
      481 ccctgtattg ggcaatgggc acgtcatacg gatgatccca cacgtaaaaa ttcaatcatc
      541 acatcattta accgcaactt tgccaaacgc aacgatggta accccaacac gcattccttt
      601 gttgcatcgc ccgaaatagt aacggcactt gccattgcag gcgatttaac ttttaatccg
      661 ataaccgata cattaaccaa tgcccaaggt atgcaagtaa aattagccga gccacaaggc
      721 attgaattac cgcttaaggg atttgaagtt gccgatgcag gctatcaggc accggcattg
      781 gatggtagta gtgtacaagt gattgtatcg ccaaccagcg accgtttgca gcttctcgaa
      841 ccttttgcag cttgggaagg aagcaactta accaacttaa aattgttgat taaagccaaa
      901 ggtaaatgta ccaccgacca catttcgatg gcaggtccat ggttaaaata tcgcggtcat
      961 ttagataaca tcagtaacaa catgcttatt ggagctgtta atgcatttaa cgataaaacc
     1021 gataatgtaa aaaatcagct taccggtatt tatggtgctg tgcctgcaac acaacgtgca
     1081 tacaaagcag caggcattgg ttccattgta gtaggtgacg aaaactatgg cgaaggatcg
     1141 agccgcgagc atgcagctat ggaaccccgt tttctgggtg tgcgtgccgt acttgtaaaa
     1201 tcgtttgccc gcatccatga aaccaatttg aaaaaacaag gcatgcttgc gcttacattt
     1261 gctaacaagg ccgattatga taaaatacag gaagatgata ac
//
LOCUS       DAOMAA010000771         1301 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_130518_length_1301_cov_2.202247, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000771 DAOMAA010000000
VERSION     DAOMAA010000771.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130518_length_1301_cov_2.202247"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1301
                     /gene="recJ"
                     /locus_tag="PLO59_09585"
     CDS             <1..>1301
                     /gene="recJ"
                     /locus_tag="PLO59_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017259547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HQV01399.1"
                     /translation="LYAKVAFYIPDRYHEGYGVSFKGVEFAQQNGYTLIIALDCGIKA
                     HDKVALANSYGIDFIICDHHRPGNDLPEALAILDPKRNDCPYPFKELSGCGVGFKLVQ
                     AIAQNNHIPFAQIEPYLDLVAVSIAADIVDIMGENRVLAHFGLKLLNTHPRFGINALI
                     NLSKVSRELSISDVVFFIAPRINAAGRIESGNRAVDLLTANDFETARIICDEINEHNT
                     YRKELDTDITEQALQQIENDLTFSDRKSTVVYSAEWHKGVIGIVASRLTDKYYRPTVV
                     MTQTNGHVAGSARSVKDFDVYNAIEACADLLDQFGGHMYAAGLTMKIENVDAFIQKFE
                     TIVSSTIEDRMLIREVDIDDALLLDDINDSFYRILKQFAPFGPGNMNPTFISTGLYNS
                     GSSRIVANKHLKLGLMQKGCNHKKDGIAFNMADCFYLIENT"
BASE COUNT          404 a          258 c          266 g          373 t
ORIGIN      
        1 tttgtatgca aaggttgctt tttacatacc cgacaggtat catgaaggtt atggcgtatc
       61 gtttaaaggt gtagaatttg cacagcaaaa cggatataca ttaataattg ccttagactg
      121 tggtattaaa gcacatgata aagttgcctt agccaatagc tacggaattg attttattat
      181 ttgcgaccac cacaggcccg gaaatgattt accagaggca ttggctattt tagaccctaa
      241 gcgcaacgat tgcccttatc ctttcaaaga actatcgggt tgtggtgtag gctttaaatt
      301 agtacaggca attgcgcaaa acaatcacat cccttttgca caaatagaac cttatttaga
      361 tttggttgcc gttagcattg ctgccgacat tgtagatatt atgggcgaaa accgggtgtt
      421 agcccatttc ggtttaaaac tgctaaatac acatccgcgc tttggtataa atgcactcat
      481 caatttatca aaagtaagcc gcgaactttc tattagcgat gtggtatttt ttattgcacc
      541 acgcataaac gctgccggac gtatagaaag cggcaaccgt gctgttgatt tacttactgc
      601 caacgatttt gaaacagcac gtattatatg tgacgaaatt aacgagcaca atacctaccg
      661 caaagagtta gataccgaca taacggaaca agcgctacag caaattgaaa acgacttaac
      721 ttttagtgac cgcaaatcta ctgtagtata ttcggccgag tggcataaag gtgttattgg
      781 tattgttgca tcgcgcctaa ccgataaata ttacaggcca accgtagtaa tgacacaaac
      841 aaacggccat gtagcaggta gtgcccgcag cgttaaagat tttgatgtat ataatgccat
      901 cgaagcctgt gccgatttgt tagaccagtt tggtggccac atgtacgctg caggtcttac
      961 catgaaaata gaaaatgtag atgcatttat tcaaaaattc gaaaccatag ttagcagcac
     1021 tatcgaagac agaatgctta tccgtgaagt tgatattgac gatgcgttgt tgttagatga
     1081 cataaacgat tcgttttacc gcatactcaa acaatttgca ccttttggcc cgggcaatat
     1141 gaatcccaca tttatttcaa ccggattata taactcaggc tcatcgcgca ttgttgccaa
     1201 taaacacctg aagttgggac ttatgcaaaa aggatgtaat cataaaaaag atggtatcgc
     1261 atttaatatg gccgattgct tttatttgat cgaaaacacg c
//
LOCUS       DAOMAA010000772         1300 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_130651_length_1300_cov_3.045783, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000772 DAOMAA010000000
VERSION     DAOMAA010000772.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130651_length_1300_cov_3.045783"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1283)
                     /locus_tag="PLO59_09590"
     CDS             complement(<1..1283)
                     /locus_tag="PLO59_09590"
                     /inference="COORDINATES: protein motif:HMM:NF028132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01400.1"
                     /translation="MQFINVAPLGGFYSVPAKPVLSKATWLDGTFAEGANTYFDFNLG
                     FRPWLIRCRNTFYYWVLQSSGQYDFMVGKHRNIFYNNNVYAATGKDFVGKEFISNRIK
                     KLAYVADKLSEKGIKLFVVLAPGKANLYTADLPMAYLPADSVNTNYYWLKKNLVANKL
                     PLIDFHEQYAQLKKTSPYPIYSYGSLHWSTYHYMHSWDSILNYINSLGGKPVSDFVIG
                     PVVQSDSASLQEMDQLPGMNVFGTLQKQTYYYPKYAYRNVPVAKAKTLFISDSFVLTF
                     MANNLTNDAMDAQCWFYHRDVYPQSYTKPLALRDINYWGELYKQDYVCVLMSEVTWGE
                     AGFEFADKVYNHLTDGINAMLIEFDSKVMSQYTFISSDLNSKQMITSKAVANNISFER
                     MAYLDAIYLINLDFTNNLGADTTYKLLFDKIAGSKL"
BASE COUNT          402 a          254 c          237 g          407 t
ORIGIN      
        1 agtttgctgc cggcaatttt atcaaacaac agtttgtaag tggtatcggc acctaagtta
       61 ttagtaaaat caaggtttat caagtaaatg gcatctaagt aagccatacg ttcgaaactg
      121 atattgtttg caacagcttt gcttgtaatc atttgcttac tgtttaaatc agatgaaata
      181 aaagtatact gactcattac tttactgtca aattcgataa gcattgcgtt tataccatct
      241 gttagatgat tatacacttt atcggcaaat tcgaaacctg cttcgcccca ggtaacttcc
      301 gacataagta cacatacata atcttgctta tacaactcgc cccagtagtt tatatcacga
      361 agtgctaaag gtttggtgta tgattgcgga tatacatcac gatgataaaa ccaacattgc
      421 gcatccattg catcgttggt taaattattg gccataaaag tgagtacaaa actatcgctt
      481 ataaataagg ttttggcttt ggctacaggc acattgcgat aggcgtattt tggataataa
      541 taggtttgct tttgcagcgt accaaaaaca ttcatgccgg gtagttggtc catctcctgc
      601 aaacttgccg agtcggattg cacaaccggg cctatcacaa aatcgctaac aggcttgccg
      661 cctaatgagt ttatataatt taaaatagag tcccagctat gcatataatg ataggtactc
      721 cagtgtaagc tcccgtaaga gtaaatagga tatggcgatg tttttttaag ctgtgcatat
      781 tgctcatgaa aatcaatgag tggtaattta tttgctacta aatttttttt cagccaatag
      841 taatttgtgt ttacgctatc tgccggcaga tatgccatgg gtaaatctgc tgtgtataaa
      901 tttgcttttc ccggtgctaa aaccacaaat aattttatgc ctttttcgct taatttatca
      961 gcaacgtagg ctaacttttt gattctattg ctgataaatt ctttaccaac aaaatctttt
     1021 cctgtagctg cgtaaacatt gttgttataa aaaatattgc ggtgcttgcc caccataaaa
     1081 tcatactgcc ctgagctttg caaaacccaa taataaaaag tgttacggca acgaattagc
     1141 cagggcctga accccagatt aaaatcaaaa tatgtatttg caccttcggc aaaagtgcca
     1201 tcaagccagg ttgctttgct taaaacaggt ttggcaggca cactgtaaaa accacccaaa
     1261 ggcgccacat taataaactg caaacgttgt tgcaataatg
//
LOCUS       DAOMAA010000773         1297 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_131232_length_1297_cov_2.863124, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000773 DAOMAA010000000
VERSION     DAOMAA010000773.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131232_length_1297_cov_2.863124"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /locus_tag="PLO59_09595"
     CDS             <1..797
                     /locus_tag="PLO59_09595"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HQV01401.1"
                     /translation="LSHSASNATKVKLGVIDRKIEASNETFEAWYQKANAFAGKNSAS
                     VDIAAFDKAAQTAGYTSRTADNIKEIDRNINGIGNNCRDVVRWIYSAKPNEVSRSFRV
                     DNKYIVAMVKYIAPQGYRTIETAKDLVTAGAIREKKAQTLIEKANAALASNKNIDALA
                     AKLNTQVIPVQSVNFGSQYVPGLGPEPKVIGQLVTLKQGEVSKPIEGSQGVFVIMAET
                     VSKPAEIKDFSSTKQTLMQQNGNRFYEVFNALKEKANVKDNRGRFY"
     gene            complement(945..>1297)
                     /locus_tag="PLO59_09600"
     CDS             complement(945..>1297)
                     /locus_tag="PLO59_09600"
                     /inference="COORDINATES: protein motif:HMM:NF015226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015204 - urea transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071918 - urea transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HQV01402.1"
                     /translation="IFFAAVLFSSPIAALYGILCSMITLPTLFYFSYNETDIYNGMLS
                     FNVVLCAITFAGKKMVDFLWAMLSSVIAICCSYILLKLNVAMLTFPFVVASCFTYYLK
                     LFLDKKKTNKPTKG"
BASE COUNT          485 a          211 c          244 g          357 t
ORIGIN      
        1 ttttaagcca ctcggcaagt aatgcaacca aagttaaact gggcgttatt gaccgtaaaa
       61 tagaagcaag caacgaaact tttgaagcct ggtatcaaaa agccaatgca tttgccggta
      121 aaaatagtgc atcagtagat attgctgctt ttgataaagc cgcacaaaca gcaggctaca
      181 cctcacgtac cgctgataat ataaaagaaa ttgatcggaa tataaatgga attggtaaca
      241 attgtcgcga tgtagtacgt tggatttata gtgccaaacc taatgaggta agcagatcgt
      301 ttcgggttga taacaagtat attgttgcta tggtaaaata tattgcacca cagggttacc
      361 gcaccataga aacagctaaa gatttggtta cagccggtgc aataagagag aagaaagcac
      421 aaactttaat tgaaaaggca aatgcagcat tagcatcaaa taaaaatatt gatgcattgg
      481 ctgccaaatt aaatacacag gttataccgg ttcaaagtgt aaattttggg tcgcagtatg
      541 tgcccggttt aggtcccgaa ccaaaagtta ttggtcaact ggtaacacta aaacaaggcg
      601 aagtttcgaa accaattgag ggctcgcaag gtgtatttgt tatcatggca gaaacagtaa
      661 gtaaaccagc cgaaataaag gactttagct ctaccaaaca aacattgatg caacaaaacg
      721 gtaaccgctt ttacgaagtg tttaatgcat taaaagaaaa agcaaacgtt aaggataaca
      781 gaggcagatt ctattaataa ttaaattgat ataattttgt cattcacaaa cagaaacagc
      841 cccaaatttt cggggctgtt tctgtttgta tctatgagct atggatgacg gttttataga
      901 ataaatttct taaaccttgt tctaattgtg gcggatgtat aaaactatcc ttttgttggt
      961 ttattggttt tctttttatc taaaaacaat tttaaatagt atgtgaaaca agatgcaaca
     1021 acaaatggaa aagttagcat agcaacattt aatttcaata atatatagct gcaacaaatt
     1081 gcaataacag atgacaacat ggcccataaa aaatcaacca ttttttttcc ggcaaatgtt
     1141 atggcacata aaaccacatt aaaactaagc atgccattgt aaatatcggt ctcgttatat
     1201 gaaaagtaaa ataatgtagg tagtgtaatc atcgaacata aaataccata taaagcagct
     1261 attggtgagc taaaaagaac tgcagcaaaa aatatta
//
LOCUS       DAOMAA010000774         1296 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_131471_length_1296_cov_2.442385, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000774 DAOMAA010000000
VERSION     DAOMAA010000774.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131471_length_1296_cov_2.442385"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="PLO59_09605"
     CDS             complement(<1..993)
                     /locus_tag="PLO59_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015809081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HQV01403.1"
                     /translation="MKLVTYKNPTPQLGILVADKIYNLSAAAQQSGFTLMPDNMRQFL
                     QGGQALMQKAKDIDAQIKSGALQISSVVFADSILDAPVNEATSCRDGYAFRQHVAAAR
                     RNRKVDMIAEFDHYPIFYFTNHNALQGPGNIYCMPDHFNKLDFELEVAVVIGKKGINI
                     KAHEADDYIAGYMIMNDMSARTLQMEEMLLNLGPAKGKDFSTVIGPCLVTPDELTPYL
                     IPAKNKHTGNNHNLNMKCWVNDVLVSEGNVGDMDWTFAEIIERCAYGAYILPGDVIGS
                     GTVGTGCFLELNGTGLLNDAHYKPQWLQPDDVVTMEITGLGKLTNTIKKMDSTFS"
BASE COUNT          383 a          260 c          243 g          410 t
ORIGIN      
        1 tgaaaaagta ctatccattt ttttaatggt attggttaat ttgcctaagc cggtaatttc
       61 catggttacc acatcatcgg gttgcaacca ctgtggtttg taatgggcat cgtttaaaag
      121 gcctgtgccg tttaactcca aaaaacatcc tgtaccaaca gtaccactac caataacatc
      181 gcccggtaaa atataagcac cgtaagcgca acgttcaata atctcggcaa aggtccaatc
      241 catatcgcca acattgcctt ccgatactaa cacatcattt acccaacact tcatatttaa
      301 gttatggttg ttgcctgtgt gtttgttttt agccggaatt aaataaggtg tcaactcatc
      361 gggcgtaact aaacaagggc caataacggt actaaagtct ttgccttttg cagggcccag
      421 gtttaaaagc atttcttcca tttgcaaagt acgggcactc atatcgttca taatcatgta
      481 accggcaatg taatcatccg cttcatgcgc tttaatgttg atgccctttt tgccaataac
      541 aacggccact tccaattcaa aatcaagctt attaaaatgg tcgggcatgc aataaatatt
      601 gccggggcct tgtaaagcat tgtggttggt aaaataaaaa atggggtagt ggtcaaactc
      661 tgcaatcatg tccactttac ggtttctgcg ggctgcggcc acatgttgtc tgaaagcata
      721 tccatccctg caactggtag cttcgttaac gggtgcatct aaaatcgaat cggcaaaaac
      781 aacactgctt atttgaagag cgcctgattt tatttgggca tcaatatctt ttgctttttg
      841 catcagtgct tggccacctt gtaaaaactg gcgcatgtta tcgggcatta gtgtaaaacc
      901 gctttgttgt gctgctgccg ataaattata aattttatct gcaaccaata tgcctaactg
      961 tggtgttggg tttttgtaag ttactaattt catgtgtaat tttattgggt gtatcccatt
     1021 tttaatttaa agcgccaaaa gtaatattta gcatcagaaa attattaaac ctgcatgcag
     1081 gcatatttaa agcaccattt ttttaggttt gcatcaaacg ctaattatgt atcacaaact
     1141 tttatttctg ttgctgataa gcctcattgc atgcaataaa aaaacaattc atggaccggt
     1201 taattttaaa gataagatag gagataaaac ctacgattgt atttacacag gtatgcaagg
     1261 gttatatacc aaacacagtt gcaattgtta tgatgc
//
LOCUS       DAOMAA010000775         1295 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_131623_length_1295_cov_3.184677, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000775 DAOMAA010000000
VERSION     DAOMAA010000775.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131623_length_1295_cov_3.184677"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="PLO59_09610"
     CDS             <1..248
                     /locus_tag="PLO59_09610"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HQV01404.1"
                     /translation="EAALMAKQQTLVTLKDNLLAKLQDEQNALNDSVNNHMQQKMKAF
                     VKGKNIAYIVGYHRSGEVLYANDSLNITDAVLLDLNK"
     gene            complement(249..>1295)
                     /locus_tag="PLO59_09615"
     CDS             complement(249..>1295)
                     /locus_tag="PLO59_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01405.1"
                     /translation="KNILNLGYIMPFYFETNALPILTDDKPTIEPQSLSALSFFQGAQ
                     LAIDSFTNENLKVVCNAYDLADSTLSLKITNDKQVNNNDFIFTTSLAPLRELLIQSPT
                     PKYFCIQPVAIAHKNLAVCQPTNQAMIKQLALFIAEKNMPVAIIFRNQKKEKELAKWI
                     QTEIDSALSFKNSLYKTSMFDHSSLKQQVLQKLDKQQKSIVLIASNDEAFIAPLIHEM
                     ASDNKLKIQIIGLPTWENYESLSFTDLQLLQTIIFNNDYLNFKDLEIQKLRKTFINKY
                     KCDLSYPSYQGFYLTKNILLNYIQIKTEWGNQKLQEVLNGHTFKFSSNQPFNGYFNTE
                     TYFLLYNNFSLQKQ"
BASE COUNT          421 a          190 c          225 g          459 t
ORIGIN      
        1 ccgaggcggc attaatggct aaacaacaaa cactggtgac tttaaaagat aatttactag
       61 ctaagcttca ggatgagcaa aatgcattaa acgattcggt aaataaccac atgcaacaaa
      121 aaatgaaagc atttgttaaa ggtaaaaaca tagcttacat cgttggttac caccgctcgg
      181 gcgaagtact ttacgcaaac gattcgctaa atattaccga tgcagtttta ttggatttaa
      241 ataagtaact attgcttttg aagcgaaaaa ttgttataca acaaaaagta ggtttcagta
      301 ttgaagtagc cgttaaaagg ttgattagag ctgaacttaa aagtatgccc atttaaaact
      361 tcctgtagtt tttgattacc ccattcggtt ttaatttgta tatagttcaa taagatgttt
      421 ttggttaaat aaaatccctg ataggaagga tagctcaaat cacatttata tttattaata
      481 aaagtttttc gaagtttttg tatttctaaa tctttaaaat ttaaatagtc gttgttaaaa
      541 attatggttt gtaataattg caagtcagta aagcttagcg attcatagtt ttcccaggta
      601 ggtaatccaa taatttgaat ttttaatttg ttgtcagatg ccatttcatg aattaaaggc
      661 gcaataaaag cttcatcatt ggaggcaatt aacacgatac ttttttgttg tttatccaac
      721 ttctgcagta cttgttgttt taaactactg tgatcaaaca tcgaagtttt gtacaaactg
      781 tttttaaatg acaaagcact gtctatttct gtttgaatcc attttgctaa ctctttttcc
      841 tttttctggt ttcgaaaaat aattgctacc ggcatatttt tttctgcaat aaatagggcc
      901 agttgcttaa tcatagcctg atttgtaggt tggcatacag ctaaattttt atgtgctata
      961 gcaaccggtt gtatgcaaaa atattttggt gtcggacttt gaatcaacaa ttctcttaaa
     1021 ggtgccagac tggtagtaaa aataaaatca ttattgttta cttgtttatc gttggtaatt
     1081 tttaatgata gcgttgagtc ggccaagtcg taagcattac aaaccacttt taaattttcg
     1141 tttgtaaacg aatcaatggc taattgtgca ccttgaaaaa aagataatgc acttaatgat
     1201 tgaggctcaa tagtgggctt atcgtcagta agtattggca atgcatttgt ttcaaaataa
     1261 aatggcatta tgtagcccag gtttaaaatg ttttt
//
LOCUS       DAOMAA010000776         1292 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_132243_length_1292_cov_3.062247, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000776 DAOMAA010000000
VERSION     DAOMAA010000776.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132243_length_1292_cov_3.062247"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="PLO59_09620"
     CDS             complement(<1..274)
                     /locus_tag="PLO59_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyltransferase"
                     /protein_id="HQV01406.1"
                     /translation="MHLHLVSFDIPFPADYGGVIDVYYKLKSLAQVGVKVHLHCFGYG
                     RKQSEELEKHCYTVTYYQRNTAKSQLFNRLPYIVVSRGNELLIQNLL"
     gene            complement(276..>1292)
                     /locus_tag="PLO59_09625"
     CDS             complement(276..>1292)
                     /locus_tag="PLO59_09625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQV01407.1"
                     /translation="NSKPLSRGYKTVRFKLWFTKGPLFYFFYNLRLFFYLYGYKPAAL
                     HANDLDTLLPNFLISKWFNIPLVYDSHEFFTGVPELQNRKWVRLIWEKIEEAIFPKLK
                     FVSTVNASIANLYQSKYNVQVSVIRNVPMAVEYHNEDKIALRQKLSLPKHAFIAILQG
                     NGINVHRGAEQAVAAMQFVDACLIIAGNGDVLPQLKQMVADLNLSDKVIFKPSMPFAD
                     LCKYTMAADLGLTLDLDTNLNYKLSLPNKLFDYIQCRIPILASNLVEVAKIIKTYDIG
                     ITINKVVPLDIAKAIHQIKGDDYIQEKWKMNLEQAALDLTWFNEKQVFCTLIEKAFGN
                     PLSN"
BASE COUNT          421 a          243 c          199 g          429 t
ORIGIN      
        1 ttaacaggtt ttgtatcagc aattcatttc cgcgcgatac aacaatgtaa ggtaaccggt
       61 taaacaattg cgattttgcc gtattacgtt gatagtaggt aactgtataa caatgttttt
      121 cgagttcctc actttgtttg cgcccatagc caaagcaatg taaatgcact ttaacgccta
      181 cttgcgctaa tgattttagt ttgtagtaaa catcaataac accgccataa tcagccggaa
      241 aaggaatatc aaaacttacc aaatgtagat gcatattaat tgcttaacgg gttaccaaat
      301 gctttttcaa tcagtgtgca aaaaacttgc ttttcattaa accaagttaa atcaagtgct
      361 gcttgttcta aattcatttt ccacttttct tgtatataat catcgccttt aatttgatga
      421 atagcttttg ctatatccag tggtactact ttattaatag taataccaat atcataggtt
      481 ttaattattt tagcaacctc taccaaatta cttgccagta tgggtatgcg gcattgaatg
      541 tagtcgaata gtttattggg taaacttagc ttgtagttta aattagtatc taaatcgagg
      601 gttaaaccca aatcggcagc catggtgtat ttacacaaat cggcaaaagg catactgggt
      661 ttaaaaatta ctttatcact taaatttaaa tcggcaacca tttgttttaa ttgcggcaaa
      721 acatctccat tacctgcaat tatcaggcat gcatctacaa actgcatagc tgcaacagct
      781 tgctcggcac cccggtgtac atttattcca tttccttgta aaattgcaat aaaagcatgc
      841 ttcggcaaac tgagtttttg tcttaaagct attttatctt cattatgata ctcaacagcc
      901 attggtacat ttctgattac gctaacctgc acattatact tactttgata taaatttgca
      961 atggaggcat ttacagtaga aacaaactta agtttgggaa aaattgcttc ttctattttc
     1021 tcccaaatca aacgaaccca cttgcggttt tgcaactcag gtacaccggt aaaaaactca
     1081 tggctatcat ataccaatgg tatattaaac cattttgata ttaaaaaatt aggcagcagt
     1141 gtatctaaat catttgcatg caatgcagca ggtttataac catacaaata aaaaaacaac
     1201 ctcaagttgt aaaaaaaata aaacaatggc cctttagtaa accataattt aaaacgtact
     1261 gttttataac ctctgcttaa tggtttgctg tt
//
LOCUS       DAOMAA010000777         1292 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_132253_length_1292_cov_2.957154, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000777 DAOMAA010000000
VERSION     DAOMAA010000777.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132253_length_1292_cov_2.957154"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1292
                     /locus_tag="PLO59_09630"
     CDS             <1..>1292
                     /locus_tag="PLO59_09630"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HQV01408.1"
                     /translation="KGLICFTKKEFKEALPFLKQAYQLANTAKNHFMPTSDFNNDCIM
                     YSQCLIFCNYPDSAIALLNNYLSTTKLEYGKMLLSQAYYCLVLAYSKKQNFDSVAFYL
                     SRFNASKDLVFTDKHAATIAKTEVSYKILLKDKEIEKLNFTNDIKTLQLKLNNKQLAE
                     ADLKNRNQILLISHSEFNRQQINQKLIDNQQALKIKNDALLIEKQNAKLTALNLYQSK
                     QRQWLLLAGIALFTVLAIYSYGRFIKHKKLNIKLDNSLKKLKEMQSLLVATEKEKATE
                     KTRAELSRDLHDDLGATLSSLSVYSMAAKGRLQNNQLTDLPHILDTIGTHAQEMVMHI
                     NERVWLLKPENNTIEKTTEQLQNYASNLLLLRNIPFVIDCSHDVSAMKLQTEAQKNVY
                     LIFKEAINNTAKYAQASMVNFIISIADNTIIFKMNDNG"
BASE COUNT          502 a          210 c          209 g          371 t
ORIGIN      
        1 taaagggctt aatttgtttt accaagaaag aatttaagga ggcattgcca tttttaaaac
       61 aagcatacca gttggctaat acagccaaaa atcattttat gccaacaagc gatttcaata
      121 acgattgcat aatgtattcg caatgtttaa ttttctgcaa ttatcccgat agtgctatcg
      181 cattgctgaa caattattta tctacaacaa agcttgaata tggtaaaatg ttactgtcgc
      241 aagcatatta ttgcttggta ttagcataca gtaaaaaaca gaatttcgac tcggttgcat
      301 tttatttaag cagatttaat gcctcaaaag atttggtgtt tacagataag catgctgcaa
      361 caattgctaa aactgaagtt agctataaaa tcttattaaa agataaagaa attgaaaagc
      421 taaattttac aaacgacatt aaaacgcttc agcttaaact taataacaaa caacttgccg
      481 aagccgattt aaaaaatcgg aaccaaatat tattgattag ccattcggaa ttcaatcgcc
      541 aacaaatcaa tcaaaaactg attgataacc aacaggcatt aaaaattaaa aatgatgcgc
      601 ttttaattga aaaacaaaat gcaaaactga cagcattaaa cctttaccaa agtaaacaaa
      661 ggcagtggct gctgcttgct ggcattgctt tgtttactgt attagccata tatagttatg
      721 ggcgttttat aaagcataaa aagttaaata ttaagctcga taattcgtta aaaaaattga
      781 aagaaatgca aagtttgtta gttgctacag aaaaagaaaa agcaacagaa aaaacccgag
      841 ccgagctttc gcgcgattta cacgatgatt taggcgccac cttaagtagt ttgtcagtat
      901 atagcatggc agccaaaggc agattacaaa acaatcagtt aactgacttg cctcatatat
      961 tagacactat cggcacacat gcacaagaaa tggttatgca tattaatgaa cgcgtttggt
     1021 tgttgaaacc tgaaaataat accattgaga aaactacgga gcaattacaa aactatgcat
     1081 caaatttatt gttgttaaga aatataccat ttgtaataga ttgtagccat gatgtttcag
     1141 caatgaaact acaaacagaa gcacaaaaaa atgtgtatct gatttttaaa gaagctataa
     1201 acaatactgc aaaatatgca caggcaagca tggtaaattt catcatatcc attgcggata
     1261 ataccatcat ttttaaaatg aacgataatg gt
//
LOCUS       DAOMAA010000778         1291 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_132439_length_1291_cov_3.078479, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000778 DAOMAA010000000
VERSION     DAOMAA010000778.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132439_length_1291_cov_3.078479"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..467
                     /gene="ybeY"
                     /locus_tag="PLO59_09635"
     CDS             3..467
                     /gene="ybeY"
                     /locus_tag="PLO59_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019946063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HQV01409.1"
                     /translation="MQLITKDINNIALHKADVSTACKNIRFTKQWLANAAKAEGYAIE
                     SLQYIFCSDKYLLAINKKFLNHHNYTDIITFEYNVEKNVIADIYISIDTVRTNAKRFN
                     VTVADELHRVMIHGLLHVTGYNDKTTALKQTMTARENHYLKKRRWIIQKQTN"
     gene            440..937
                     /locus_tag="PLO59_09640"
     CDS             440..937
                     /locus_tag="PLO59_09640"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HQV01410.1"
                     /translation="MDYTKANKLIQYNLWANSLLMQWVIAAGNAAADTEVKSSFNTLR
                     NTIYHIYDAQYVWFLRVQNQPLLHWPPSSNFNYTLAQFDIVLQQQSNDWLQFIATLKP
                     NEFEQTIVYTNTKNQPFETTIADIITHWINHGTYHRGQCITMLHQLGFNAVGSTDYIT
                     FCRLK"
     gene            955..>1291
                     /locus_tag="PLO59_09645"
     CDS             955..>1291
                     /locus_tag="PLO59_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQV01411.1"
                     /translation="MQYINLTDAELLLLCTTKVDMEALGILFSRHRLMALGVCMKYLK
                     DEDESKDAVMQVFEKLITDLPKHQIQNFKPWLHTVLKNHCLMQLRKNKSALTFESVEN
                     IASVVENVTD"
BASE COUNT          459 a          265 c          222 g          345 t
ORIGIN      
        1 caatgcaact aatcaccaag gatataaaca acattgcgct tcataaagcc gatgtatcaa
       61 cggcatgcaa aaatataagg tttacaaagc aatggttggc aaatgcggca aaagccgaag
      121 gttatgccat cgaatcgctt cagtacatat tttgcagtga taaatattta ttggctatca
      181 acaaaaagtt tttaaaccat cataattata ccgacatcat taccttcgaa tacaatgttg
      241 aaaaaaacgt aattgccgat atttacatca gcattgacac agtacgcacc aatgcaaaac
      301 gattcaatgt tactgttgca gatgaactac accgcgtaat gatacatggc ttgctgcatg
      361 taaccgggta taacgataaa acaacggcat taaaacaaac catgacagca cgcgaaaacc
      421 actacttaaa aaaacgcaga tggattatac aaaagcaaac aaactgatac aatacaacct
      481 ttgggctaac agccttttga tgcaatgggt aatagcggcc ggcaacgcag cagccgatac
      541 cgaagttaaa agcagtttta atacattgcg taataccata taccatatat atgacgcaca
      601 atatgtttgg tttttacgag tgcaaaatca accgctgtta cattggcctc ccagcagtaa
      661 ttttaattac actttagcgc aattcgatat cgttttgcaa caacagtcga atgattggtt
      721 gcaattcata gcaactttaa aacctaatga attcgaacaa actattgttt acaccaatac
      781 taaaaaccaa cccttcgaaa ctaccatagc cgatataatt acccattgga taaaccatgg
      841 tacttaccac cgcggccaat gtataaccat gttgcaccaa ttgggtttta atgccgttgg
      901 tagcaccgat tatattactt tttgccgtct taaataaaaa gccaaacaat accattgcag
      961 tacattaatt taaccgatgc cgaattgtta cttctatgca caactaaagt ggatatggaa
     1021 gcattgggta ttttatttag tcgccaccgt ttaatggcat tgggtgtgtg catgaaatat
     1081 ttaaaggatg aagacgaaag caaagatgct gtaatgcagg ttttcgaaaa actgattacc
     1141 gacctgccca aacatcaaat tcaaaacttt aaaccctggt tgcatacagt gcttaaaaat
     1201 cactgcctga tgcagttacg caaaaacaaa agtgctttaa cattcgaatc ggtcgaaaat
     1261 attgcctccg ttgtggaaaa tgtaaccgat t
//
LOCUS       DAOMAA010000779         1289 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_132954_length_1289_cov_1.832253, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000779 DAOMAA010000000
VERSION     DAOMAA010000779.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132954_length_1289_cov_1.832253"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1234)
                     /locus_tag="PLO59_09650"
     CDS             complement(<1..1234)
                     /locus_tag="PLO59_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01412.1"
                     /translation="MVRKEISNTYIIGANGGQVKVYSAATDGVNIYAATDEGVKHADY
                     NSTNLQNFNNWSLFSLADSLPKKKATYVATFNNKAYAVIGTNNTDTLYEFNGTYWSYK
                     YSDLNNTFSSVSANSNTLYFTTQGNVVTDGKNGKIDASGNVSANLAQHPRPTGWFEGN
                     GTNWEGDLYVGLIKNNNGNIETIVPNGPFSTRVSDLEIENGVLYVSSGGADDIWAPLF
                     IKDGFFVYNNSSWTNYNLYTNPELDTFPGIVAIVDIPEKNQVYLASFLRGLGVFDKTT
                     KKVTIINKDNNLLYNSFFELTSNTTLDTRLSAMAKDKYNNLWICNAGADKAIKLLKNT
                     GDWAQFRIPYNYGLMKKIVIDQNDQLWAPLRTLGAGLLVWSYNQTIDDPSDDVSRVLY
                     TGAGAGGLPDANVYCLAED"
BASE COUNT          376 a          270 c          236 g          407 t
ORIGIN      
        1 tatcttcggc caagcagtaa acgtttgcat cgggtaatcc gcctgctccg gcaccggtat
       61 aaagtacgcg cgaaacatcg tctgaaggat catctatagt ttgattataa ctccaaacta
      121 aaagccctgc ccctaacgtt cttaatggtg cccataactg atcattttga tcaattacaa
      181 ttttcttcat caaaccataa ttgtagggta ttctaaactg agcccaatca ccggtgtttt
      241 tcaataattt aattgcctta tctgcccccg cattacatat ccacaagttg ttatacttgt
      301 ctttagccat tgcgcttaac cgggtatcga gcgtagtgtt tgaggttaac tcaaaaaatg
      361 aattgtaaag cagattattg tctttattaa tgatagtaac ctttttagtg gttttgtcga
      421 acactccaag cccacgtaaa aaagaagcca gataaacctg gtttttttcg ggaatatcta
      481 ctatggctac tattccggga aacgtatcca actcgggatt tgtgtacaga ttgtaattgg
      541 tccaggagct attattgtaa acgaaaaatc catcctttat aaaaagcggt gcccaaatat
      601 catccgcccc accggatgat acatacaaca ccccattctc gatttcaaga tcggaaacgc
      661 gggtagaaaa cgggccattt ggaactattg tttcaatgtt gccattatta tttttaataa
      721 gaccaacata taaatctccc tcccaattag ttccgtttcc ttcaaaccaa ccggttggcc
      781 gggggtgttg ggctaaattt gcagaaacat tacctgatgc gtcaatctta ccattttttc
      841 catcggttac cacattgcct tgtgtggtaa aatacaacgt attgctgttt gccgaaacac
      901 ttgaaaacgt attgtttaag tcagaatatt tataagacca gtaggtgcca ttaaattcgt
      961 aaagcgtatc ggtgttattt gttcctataa cggcgtacgc tttgttatta aaagtggcta
     1021 cgtaggtcgc tttcttttta ggaagactat cagctaaaga aaacaacgac caattgttga
     1081 agttttgaag attagtggag ttatagtctg catgttttac gccttcatcg gtagcggcgt
     1141 atatattaac gccatcagtg gctgcagaat atacttttac ctgaccccca ttagccccaa
     1201 taatataggt gttactaatt tctttacgga ccatattaat aactgttatg ccaatatcgg
     1261 atgatatata agcatctcca ttgtaaaac
//
LOCUS       DAOMAA010000780         1288 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_133042_length_1288_cov_3.007299, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000780 DAOMAA010000000
VERSION     DAOMAA010000780.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133042_length_1288_cov_3.007299"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1288)
                     /locus_tag="PLO59_09655"
     CDS             complement(<1..>1288)
                     /locus_tag="PLO59_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01413.1"
                     /translation="IAGTSVANTITVSRTGLPADLRYRGTDAGNLGNQASVSIIGTTF
                     EPILAVIGTDFMSVNNVNFTYNGVQGTGVSYQTTTGKVDCAIGLINSSATDGATNNTF
                     SNFVCTNDRTNPASTQAIRHFTQTTPTSAAGANSNNTYRDFTIKNGTSGIIISGNATF
                     PDINNRVITSACTSYNTIGDPAVANDLSNPSTATSPFGITMSNQSGFTLRNNIISNLT
                     NTSTGSTIVDGINVTLFQGICEINNNIIKNLRQNLGSGTSVVSGIKITNATTGTHEIR
                     VYNNAISNLTTAYNGAASVTRMIRGMSGLSTTNNAAKYSIYNNSISIDGSGASTASNN
                     CFDVAGTTGPNYDVRNNIFANYTIAQAGVARHACYVTNSNTLLGGTGSTINQNNMYVA
                     NDAGVTGFPVYNSSTNTGTNPIATVNTNYSTQVTAPN"
BASE COUNT          371 a          231 c          244 g          442 t
ORIGIN      
        1 gtttggtgct gtaacctgtg tcgaataatt cgtgtttact gttgcaattg ggttggttcc
       61 tgtatttgta ctcgaattat atacagggaa acccgttaca cctgcatcat tagccacata
      121 catattattt tggttgatgg tagaaccggt accgcctaat aaagtattgc tattggtaac
      181 ataacaagca tgtcttgcaa cacccgcttg cgcaatggta tagttagcaa aaatattatt
      241 tcttacatca tagttagggc ctgttgtacc ggcaacatca aaacaattgt ttgatgccgt
      301 tgaagcacca ctgccatcta tacttatact attattgtaa atactatatt ttgcagcatt
      361 gtttgtagta cttaaaccgc tcatccctct aatcatcctt gtaactgaag ctgcaccatt
      421 ataagctgtt gttaaatttg aaatagcatt attataaact ctgatttcat gggtacctgt
      481 tgttgcattt gtaattttaa tacctgatac tacagaagta cctgatccca aattttgtcg
      541 cagatttttt ataatgttat tattgatttc gcatatccct tgaaaaagtg taacattaat
      601 accatcaact atggtagaac ctgtacttgt gttggtcaga ttagaaatta tattatttct
      661 taatgtgaaa ccactttgat tagacatagt aataccaaaa ggagatgttg cagttgatgg
      721 gttacttaag tcgttagcaa cagcagggtc gcctatcgta ttataagaag tacatgctga
      781 tgtaattacc ctgttgttaa tatcagggaa agttgcatta ccactaataa ttataccgct
      841 tgtaccattt ttaatggtaa aatctctata agtgttatta ctattggcac cagctgccga
      901 tgtaggtgtt gtttgtgtaa aatgtctgat tgcttgtgtt gaagcagggt tggtgcggtc
      961 gttagtacat acaaaatttg agaatgtatt attggtggcg ccatcagttg ccgatgagtt
     1021 gattaagcct atggcacaat caactttacc tgttgttgtt tgataactaa ctcctgtacc
     1081 ttgtactcca ttgtatgtaa agttcacgtt gttcaccgac ataaagtcgg taccgataac
     1141 agccaaaatt ggttcaaagg ttgttccaat aatagaaaca gatgcttgat tacctaagtt
     1201 acccgcatcg gtaccacgat atcttaaatc ggctggcaat ccggttctgc ttacagtaat
     1261 tgtattggca actgaagtac ctgcaata
//
LOCUS       DAOMAA010000781         1286 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_133532_length_1286_cov_2.185215, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000781 DAOMAA010000000
VERSION     DAOMAA010000781.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133532_length_1286_cov_2.185215"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(185..>1286)
                     /locus_tag="PLO59_09660"
     CDS             complement(185..>1286)
                     /locus_tag="PLO59_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HQV01414.1"
                     /translation="LAIDAFGPIADNAGGIAEMSELPKDVREKTDVLDAVGNTTAATG
                     KGFAIASAALTALALFAAFVGIAGIEAIDIYKADVLAGLFVGAMIPFIFSSLAIAAVG
                     RAAMAMVEEVRRQFRTIPGIMEGKGKPEYDKCVAISTQASLREMMLPGSIALIVPVVV
                     GFTMGAEVLGGLLAGVTVSGVLMGMFQNNAGGAWDNAKKSFEKGFEHNGVMHYKGSDM
                     HKASVTGDTVGDPFKDTSGPSMNILIKLMSIVSLVIAPSLSHLHPVAAGHGSNTEACC
                     QTSGAETKGCCKDGVTESTDAACDMTQCHQMTQEQCAAYCDSIGCSEAQKAECLKHCK
                     DAPVADACVCKHGCATASECAEKCGEKCAAKH"
BASE COUNT          347 a          308 c          258 g          373 t
ORIGIN      
        1 ggctaaagtg tgtgttcaca ctcaatcgtg tatgcattgc cgtagtgaag tggaaagcgg
       61 aaaaggatta gtgcgttgtt taaataaatt aatttgccct aattttttta acctgaatag
      121 ctaaaatttg gtcataaaaa aaagccgctt gtttaaggcg gcttttttta tactctttga
      181 tttcttaatg ttttgctgca catttctcac cgcacttttc ggcacattcc gatgctgttg
      241 cacaaccgtg tttgcaaaca caggcatcgg caacaggggc gtctttgcag tgttttaaac
      301 attcggcttt ttgtgcctcg ctacaaccaa tgctatcgca gtaagctgcg cattgttctt
      361 gtgtcatttg gtggcattgc gtcatatcgc aagctgcatc ggtcgattct gttaccccat
      421 ctttacaaca tcctttggtt tcggcacccg atgtctgaca acatgcttcg gtattagaac
      481 cgtggccagc tgcaaccgga tgcaaatgtg ataatgaagg cgctattact aatgaaacga
      541 tggacattaa tttaatcaag atattcattg acgggcctga ggtatcttta aaaggatcgc
      601 caacggtatc gcctgttacc gaagctttat gcatatcgct gcctttataa tgcattacgc
      661 cattgtgctc aaaacctttt tcgaaactct ttttcgcatt atcccatgca ccaccggcat
      721 tgttttgaaa catacccata agtacaccag atacggtaac acctgctaat aagccgccta
      781 atacttcggc acccattgta aagccaacca caaccggtac aataagcgca attgaaccgg
      841 gcaacatcat ttcgcgtaaa ctggcttgtg ttgatatggc tacgcattta tcgtattcgg
      901 gtttgccctt accttccata ataccgggta tggttctaaa ctgcctgcgt acttcctcaa
      961 ccatggccat agcggcacgg cctacggcag caatggctaa cgatgaaaat ataaacggaa
     1021 tcatggcacc cacaaacaac cctgccaata catcggcttt ataaatatca atggcttcga
     1081 tacctgcaat acccacaaaa gctgcaaaaa gtgccaatgc tgttagcgct gccgatgcaa
     1141 tggcaaaacc tttaccggta gcagcggttg tattaccaac tgcatctaat acatctgttt
     1201 tttcgcgtac atctttgggt agttcgctca tttcggcaat accaccggca ttatcggcaa
     1261 tagggccaaa ggcatcaata gctaat
//
LOCUS       DAOMAA010000782         1285 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_133649_length_1285_cov_2.943902, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000782 DAOMAA010000000
VERSION     DAOMAA010000782.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133649_length_1285_cov_2.943902"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            170..>1285
                     /locus_tag="PLO59_09665"
     CDS             170..>1285
                     /locus_tag="PLO59_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01415.1"
                     /translation="MKTFVKGFVWVTGILLLLLVLVTSIAYWQKDKLINYVLGDINQQ
                     LQSKINIASIDFNVIKHFPNTSLVLNKVYAAPAKPFLQSDTLLAADKIYLLFDILQLI
                     NGKYQLKKVVIESAIIKLQIEKRGLRNFDIWKSDNAPAKSNLNLQFKEVLLKQTAFVF
                     DDFKQTTHYNAYFNNAQLQGNFSEKQFEVKAKANLWLHQLQVAGIHLNQSRQAMLDCN
                     IKVNRNQNEITFLPSQIKIEGIDLGIAGKLKFLNDTTQYGLTLNGNQIDLIALASVWP
                     VVAKTTNQYSASGLVSFKATINGYQTIKTKPKIEAHFSADNAALKASNDAQLTHISFN
                     GFYSNSYNGVDLLTLNKVSCQLNAQPLTGAFTVSNFKT"
BASE COUNT          475 a          194 c          227 g          389 t
ORIGIN      
        1 atagaataga aataacttca acaaaaaaag aatataaaca tattattaaa aattgaaagt
       61 atgtagaaaa tataatgtaa aagttgctgt taaaggcttt attgaagcac ttttaaattg
      121 gttgtaagtt agttaccgct atgtaaaaag cagtttggtt taaataagca tgaaaacatt
      181 tgtaaagggt tttgtttggg taacaggtat attgctgttg ttattagtat tggttacaag
      241 tatagcctat tggcaaaaag acaaactgat aaactatgtg cttggcgata taaaccaaca
      301 attacaaagt aagataaata tagccagcat tgactttaat gtgataaaac attttccaaa
      361 tacaagcctt gtattaaata aagtttatgc ggcacctgcc aaaccgtttt tgcaatcaga
      421 tacattattg gcagctgata aaatatacct gttgtttgat attttgcaat taataaatgg
      481 caagtatcaa cttaaaaaag tagtaataga atcggctatt ataaagttgc aaattgagaa
      541 gcgtgggctt cgaaattttg atatctggaa aagcgataac gcaccggcaa aatcgaacct
      601 taatttacag tttaaggaag tcttgttgaa acaaacagct tttgtgtttg acgacttcaa
      661 gcaaacaaca cactataacg catattttaa caatgcacaa cttcaaggaa atttcagcga
      721 aaaacaattt gaagtaaaag ccaaagctaa tttatggttg caccaattgc aggttgccgg
      781 tattcattta aatcaaagcc ggcaggctat gcttgattgt aatattaaag taaatcgaaa
      841 tcaaaatgag ataacctttt tgccatcgca aattaaaatt gagggcattg atttaggtat
      901 tgccggcaaa ctaaaatttt taaacgatac tacacagtat ggcttaacac ttaacggcaa
      961 tcagatagat ttgattgcat tggcatcggt ttggcctgta gtggcaaaaa caacaaacca
     1021 atacagcgct tcgggtttgg taagctttaa ggcaaccata aacggttatc aaactataaa
     1081 aacgaaacca aaaattgaag cacactttag tgctgataat gctgcgctta aagcttcgaa
     1141 tgatgcacaa cttacccata tttcgtttaa tggtttttat agtaacagtt acaatggtgt
     1201 tgatttgctt actttaaata aagtaagttg ccaactgaat gcacaaccct taaccggtgc
     1261 ttttacagtt tcaaatttta aaacg
//
LOCUS       DAOMAA010000783         1285 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_133700_length_1285_cov_2.287805, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000783 DAOMAA010000000
VERSION     DAOMAA010000783.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133700_length_1285_cov_2.287805"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="PLO59_09670"
     CDS             complement(<1..580)
                     /locus_tag="PLO59_09670"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HQV01416.1"
                     /translation="MLTALTQTADFIKSKTGFTPDVGIILGSGLGGLVAKMQIEHTLP
                     YSELPNFPVSTVAGHGSRLIMGTLGNTKVVVMQGRFHFYEGYTFEQLTFPVRVLKFLG
                     IKKLFLSNAAGGMNTNFKVGDLMVIDDHINLMPGNPLIGKNIDELGPRFPDMSAPYDI
                     DLQNQALQIAAKKGYRCHTGVYAAVSGPTYETR"
     gene            complement(552..>1285)
                     /locus_tag="PLO59_09675"
     CDS             complement(552..>1285)
                     /locus_tag="PLO59_09675"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HQV01417.1"
                     /translation="VAVAEKRVTGVDALIQKGMQALVLDDAYQHLAVKAGLYILLTDY
                     NNLYCNDYLLPAGNLREWRSAAHRAQIIMVTKCPVDISPADKNKITTALKLQQHQHLF
                     FSSIAYAPILNVLNALPALNNHFADLINYDCLLITGIANPQPLIVYCQKHFAQVHTLT
                     FADHYMFNENDLSQGRKIFDNIANSKKLILTTQKDWMRLQQGHLKIVLNDLPVYVQPI
                     SMQIDNIETFNQIIHTYVNSTHTNG"
BASE COUNT          358 a          248 c          268 g          411 t
ORIGIN      
        1 cacgggtttc ataggtaggg ccgctaacgg ctgcatacac accggtatgg catcggtaac
       61 cttttttagc agcaatttgt aaagcttggt tttgcaaatc tatatcatac ggtgcactca
      121 tatccggaaa acgggggccc agttcatcta tattttttcc gattaacgga ttgccgggca
      181 tcaggtttat atggtcgtca attaccatta aatcgcccac tttaaagttg gtattcatac
      241 cgcctgctgc attcgaaagg aataattttt ttatgcccaa aaactttaaa acccgtaccg
      301 gaaaagtaag ttgttcaaat gtatagcctt cgtaaaaatg aaagcggcct tgcattacta
      361 ccacttttgt attacccaat gtacccataa ttaaacggct gccatgcccc gctacggttg
      421 ataccggaaa gttgggcaat tcgctgtatg gtaatgtgtg ttcaatttgc attttagcaa
      481 ccaggccacc caagcccgag cctaaaataa tgcctacatc aggcgtaaaa ccggttttgc
      541 tcttaataaa atcagccgtt tgtgtgagtg ctgttaacat aggtatgtat tatttggtta
      601 aaggtttcaa tattgtcaat ttgcatactg atgggttgta cataaacagg caaatcgttt
      661 aaaactattt ttaagtggcc ttgttgcaaa cgcatccagt ctttttgtgt tgtaagtatc
      721 agctttttac tgttggcaat gttatcaaat attttgcggc cttggcttaa atcgttttcg
      781 ttaaacatgt aatggtcggc aaaggtaagt gtatgtactt gggcaaaatg tttttggcaa
      841 taaacaatta acggttgcgg atttgcaatg cctgtaatta ataagcaatc gtaattaatt
      901 aaatcggcaa aatggttgtt taatgccggc aatgcattca gcacatttaa aatgggtgcg
      961 tatgcaatgc tgctaaaaaa taaatgttgg tgctgttgta actttaaggc tgttgtaatt
     1021 ttgtttttat cggccgggct tatatcaacc ggacattttg ttaccataat tatttgtgca
     1081 cgatgggctg cactgcgcca ttcgcgtagg ttaccggccg gtaataagta atcgttacaa
     1141 tataaattat tgtaatcggt aagcagtatg taaagtccgg ctttaacggc taaatgttgg
     1201 taagcatcgt caagcactag agcttgcata cctttttgta tcaaggcatc tacaccagtt
     1261 acccgctttt cggccacagc cacca
//
LOCUS       DAOMAA010000784         1285 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_133744_length_1285_cov_1.881301, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000784 DAOMAA010000000
VERSION     DAOMAA010000784.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133744_length_1285_cov_1.881301"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1285
                     /locus_tag="PLO59_09680"
     CDS             <1..>1285
                     /locus_tag="PLO59_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01418.1"
                     /translation="TCALIGFMLYANTLNHEYAFDDFPTIYGNRITMMGFAGIPTLLK
                     TAFWYGLDGKNDWLYRPLSMVMFATEWGLAPNKPALSHWVNVLVYALTGFFLFRFLLD
                     LLSNYNILIPLGITVLWLAHPIHTEVVANIKSRDELMCFLFSVLYLHQILKYFENKQI
                     YNLLLACLYFFLALMSKESGITILAATPLMLWCFKNATIKTCITLTCITLIPAALYIF
                     IRGLVLTSQLNLGDAIPIIDNSLVGAKGDFNLEKGTAFYILGRYILLLFFPHPMSSDY
                     SFNAIPLVGFSNLLALTALILHVAAGIYALYLLPKRNAYAFGILFYLATISVVANVLF
                     LTRSTMADRFLYMPSLGFILILILMLAKISKLSFTKITFGSLNHLLQFNKTFSYALTI
                     ILVLCFGKTWSRNKDWKNDTSIFSADVEHEPNSSRL"
BASE COUNT          373 a          215 c          225 g          472 t
ORIGIN      
        1 aacatgtgcc ttaattggtt ttatgctata tgccaataca ttaaatcatg aatatgcttt
       61 tgatgatttt ccaaccattt acggtaacag aataaccatg atgggctttg ccggaattcc
      121 tactttatta aaaacagctt tttggtatgg tttagatggc aaaaacgatt ggctttacag
      181 gccattaagt atggtaatgt ttgcaactga atggggcttg gcgccaaaca aacctgcatt
      241 aagccattgg gtaaatgttt tggtttacgc tttaaccgga ttttttttgt ttcgattttt
      301 attggatctt ttaagcaatt acaacatttt aattccatta ggtatcacag tgctttggct
      361 tgcacatccc atacataccg aggttgtagc taacattaaa agccgtgatg aattaatgtg
      421 ctttctgttt tcagttttat acctgcatca aattttaaaa tatttcgaaa acaaacagat
      481 ctataattta ttgttagcct gtttatattt ctttttagca ttgatgagta aagaaagtgg
      541 cattaccatt ttagcagcaa cacccttaat gttatggtgc tttaaaaatg ccaccattaa
      601 aacatgtata acactaacat gtataacgtt aatacctgca gcattgtata tttttatacg
      661 gggattggtt ttaaccagtc agttaaattt aggcgatgcc atacctatta ttgataattc
      721 gttagttggt gccaaaggcg attttaacct agaaaagggc actgcatttt acattttagg
      781 ccgctatata ctgctcttgt tttttccaca ccctatgagc agcgattata gttttaatgc
      841 cataccattg gttggtttta gtaatctgct tgcacttaca gcccttatat tacatgttgc
      901 agcgggtatt tatgctttgt atttgttgcc caaaagaaac gcctatgcat ttggcatttt
      961 gttttatctg gctaccataa gtgtagtggc gaatgttttg tttttgacaa gaagcacaat
     1021 ggccgacaga tttttataca tgccatcatt aggttttatt ttgatattga ttttaatgct
     1081 tgcaaagatt agcaagctct cttttaccaa aattactttt ggctcgttaa accatttact
     1141 tcagtttaat aaaacattta gttatgcatt aactatcata ttggttttat gttttggaaa
     1201 aacttggagt cgaaataaag attggaaaaa cgatacttcc atatttagtg ccgatgttga
     1261 acacgaacct aacagctcac gtttg
//
LOCUS       DAOMAA010000785         1281 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_134460_length_1281_cov_2.783034, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000785 DAOMAA010000000
VERSION     DAOMAA010000785.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134460_length_1281_cov_2.783034"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="PLO59_09685"
     CDS             complement(<1..358)
                     /locus_tag="PLO59_09685"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQV01419.1"
                     /translation="MRKLSICLMLLFVAWHAVAQKQTYTTQSKAAVKAYEQATKYYDS
                     RHNTAAITSLKQAIDKDAQFIEAHMLLADVYVDESRYDDAIASYKTAVNINPKFFPNN
                     WYRLAQIEMRQGQYEQA"
     gene            complement(396..1010)
                     /locus_tag="PLO59_09690"
     CDS             complement(396..1010)
                     /locus_tag="PLO59_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019946612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HQV01420.1"
                     /translation="MAAEHKIAWPVMEHFYTIQGEGANTGKPAYFIRLGGCDVGCVWC
                     DVKESWDANKHPLTDLKTMLRWIKASKAQNVVITGGEPMMYDLTALTTALKSEGLQLW
                     LETSGAYPISGVWDWICVSPKKFKQPILQALQQAHELKVVVYNKSDFDWALQHQKSTS
                     PHCILFLQAEYSRLQQMMPQIVDFVKQHQQWRISLQTHKVIEVP"
     gene            complement(1010..>1281)
                     /locus_tag="PLO59_09695"
     CDS             complement(1010..>1281)
                     /locus_tag="PLO59_09695"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013666707.1"
                     /note="catalyzes the formation of
                     5,10-methenyltetrahydrofolate from
                     5,10-methylenetetrahydrofolate and subsequent formation of
                     10-formyltetrahydrofolate from
                     5,10-methenyltetrahydrofolate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HQV01421.1"
                     /translation="ADIVIAAMGKPQFLKADMIKTGAIVIDVGITRITSTQTKSGFKI
                     VGDVDFDDVSKKCSYITPVPGGVGLMTIVGLLQNTLLAAKKSFVY"
BASE COUNT          345 a          267 c          245 g          424 t
ORIGIN      
        1 ttgcctgttc gtattgaccc tggcgcattt ctatttgtgc taaccgatac cagttgttcg
       61 gaaaaaattt agggttaata ttgacagctg ttttgtaact ggcaatagcg tcatcgtatc
      121 tgctttcatc tacataaaca tcggccagca acatgtgggc ttcaataaac tgcgcatcct
      181 tgtcaattgc ttgttttagc gatgttatgg ctgctgtgtt gtggcggctg tcataatatt
      241 tagttgcctg ttcataggct ttaacggcag ctttactttg tgttgtatat gtttgttttt
      301 gtgctactgc atgccatgca acaaacaata acatgaggca tatacttagt ttacgcatag
      361 cgaagttttt aataaaaata tcagacacga aattattaag gtacttcaat taccttatgt
      421 gtttgcaatg aaatgcgcca ttgttggtgc tgcttaacaa aatcaacaat ttgcggcatc
      481 atctgctgta agcggctgta ttcggcctgc agaaaaagta tgcaatgtgg cgatgtgctt
      541 ttttgatgtt gcaaagccca atcgaaatct gatttattgt acacaacaac tttaagttca
      601 tgtgcttgtt gcaatgcttg caatattggt tgtttaaatt ttttaggcga aacacaaatc
      661 caatcccaaa cacctgaaat cgggtatgca cccgatgttt cgagccacaa ttgcaaacct
      721 tctgatttta atgcagtggt taaggctgtt aagtcataca tcatgggctc gccaccggtt
      781 ataactacat tttgtgcctt gcttgctttt atccatcgta gcatggtttt caaatcagtt
      841 aaaggatgtt tgtttgcatc ccaactttct ttcacatcgc accaaacaca acccacatcg
      901 caaccaccta agcgtataaa gtatgcaggt ttgccggtgt ttgcaccttc gccttgtatg
      961 gtataaaaat gctccataac aggccatgct attttgtgtt cggcagccat taatatacaa
     1021 acgatttttt tgctgctaat aatgtgtttt gcaataggcc aactatggtc attaaaccta
     1081 caccaccggg taccggtgta atgtagctgc actttttact tacatcatca aaatcaacat
     1141 cgcccacaat cttaaaaccc gacttggttt gtgtggatgt tatgcgtgta atgcctacat
     1201 caataacaat ggcacccgtt ttaatcatat cggctttcag aaactgaggt ttacccattg
     1261 cagcaattac aatatcggct t
//
LOCUS       DAOMAA010000786         1280 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_134664_length_1280_cov_2.941224, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000786 DAOMAA010000000
VERSION     DAOMAA010000786.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134664_length_1280_cov_2.941224"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="PLO59_09700"
     CDS             complement(<1..387)
                     /locus_tag="PLO59_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01422.1"
                     /translation="MKIFWISLMFFLFAGAVVAQKQSDYCLPSENKKAIKFFEDALKE
                     SKDNRDYDKIKKYVYSAAETDTTYAAPWRLLGDIAYRKDDFKTFGNAYARLIELCVDA
                     GAETYYRLGNYYYGLKKYEQAQKIFKA"
     gene            622..>1280
                     /locus_tag="PLO59_09705"
     CDS             622..>1280
                     /locus_tag="PLO59_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01423.1"
                     /translation="MKKLKLYLFVIACMLLSTYHAQAVLVSWTNNAGGNWSTGSNWSN
                     GTGPGTNDTARIILNGTYTVTLDADPTIRHLIIGGNTGRQTLSITSHALTTTHGVLVN
                     DKGIINATASTFIGNDALVNNGKIEMSYSTTFNIPLTNNDTIDINSYNNYINQNLICN
                     LGSIIRLLGNHYTTNVTVANGFTNNGTIQFNSILGYTCDLYVTNGQLINAGNGNIVSN
                     S"
BASE COUNT          411 a          231 c          220 g          418 t
ORIGIN      
        1 ggctttaaat attttttgtg cttgctcata ttttttcaag ccataataat aatttcctaa
       61 gcgatagtat gtttcggcac ctgcatcaac acacaattcg ataaggcgtg cataagcatt
      121 gccgaaggtt ttaaaatcgt ctttacggta agcaatatcg cctaacaaac gccagggcgc
      181 agcataggtg gtatctgttt cggcagcact ataaacgtat tttttaattt tatcatagtc
      241 gcggttgtct ttactttctt taagcgcatc ttcaaaaaat ttaattgctt ttttattttc
      301 agaaggcaag caataatctg attgcttttg tgcaacaacg gcacctgcaa aaagaaaaaa
      361 catcaaacta atccaaaata ttttcatggc cgaaatgtga aaacttttat gtttaagaaa
      421 agtgaatttc ctgttataaa tcattttata gtttaattag aattaaaaaa ttagtgccca
      481 aaattcagtt tagttaaaac gctacttgtt tttttagttt gcatccgtac cagtgggtat
      541 ataaaatacc gttcattggt tatttactta caaatttttc acacctaatt ttacctcatt
      601 attaacgcaa cttccttatt tatgaaaaaa cttaaacttt acttatttgt aattgcctgt
      661 atgttgctta gcacatacca tgcgcaagct gtattggtta gctggactaa caatgccggt
      721 ggtaactgga gtaccggtag caactggagt aacggtaccg ggccgggtac taacgatacg
      781 gctcgtatta ttttaaacgg aacctataca gttacacttg atgccgaccc aactatacgg
      841 catctgatta ttggtggtaa caccgggcga caaacgttaa gcattacaag ccatgcgcta
      901 acaactactc atggtgtttt agttaatgat aaaggaatta tcaatgccac ggcatcgacc
      961 tttattggta acgatgcatt ggttaataat ggtaaaatag agatgagtta ctctaccacg
     1021 tttaatatac cgctaactaa taacgatacc attgatataa attcgtataa caattacatc
     1081 aatcaaaatt tgatttgtaa cttaggtagt atcatacgct tgttgggcaa ccattataca
     1141 accaatgtta cggtagctaa tggatttaca aacaatggta ccatacagtt taattcgata
     1201 ttaggctaca cttgcgattt gtatgtaacc aatggccaac ttataaatgc cggtaatggt
     1261 aacattgtaa gtaacagtaa
//
LOCUS       DAOMAA010000787         1280 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_134717_length_1280_cov_2.368980, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000787 DAOMAA010000000
VERSION     DAOMAA010000787.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134717_length_1280_cov_2.368980"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="PLO59_09710"
     CDS             complement(<1..366)
                     /locus_tag="PLO59_09710"
                     /inference="COORDINATES: protein motif:HMM:NF027592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4920 domain-containing protein"
                     /protein_id="HQV01424.1"
                     /translation="MNIMKKGIVLLSLTYLFACNPSTEKKVDATMQTETIANSYGEKI
                     EKQTGITVAALAAQMQNKAALEDMVIEAKITEVCQEMGCWMNVEKGDGTQMFIKMKDH
                     EFFVPKDAAGKIALISGTAV"
     gene            575..>1280
                     /locus_tag="PLO59_09715"
     CDS             575..>1280
                     /locus_tag="PLO59_09715"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HQV01425.1"
                     /translation="MKKIIATCMAIIICATFLHGQINLQQPNFVDYLISSGWNQPAGI
                     VYDCTGQSYIWVLKGKIYVVDSAGNKITTPLLNIAEEVGNWRSHGLHGVCLDPNFRTN
                     GYFYCYYAVDRYHLLNFGKPNYNANSNAYYDASILRVTRFTANSATNFTTLVPNSRLV
                     LLGEDKKTGVPLLHVTHDGGTILFGRDGSLLVFTGDCADYAQNDVGSAPGTYWAQALA
                     DTIIRTAENVGDFRSQL"
BASE COUNT          371 a          236 c          223 g          450 t
ORIGIN      
        1 aacagctgta ccactaatca aagcaatttt tccggctgca tcttttggta caaaaaattc
       61 gtgatctttc atcttgataa acatttgtgt gccatcacct ttttcaacat tcatccagca
      121 tcccatttcc tgacatacct cggtaatttt tgcttcaata accatatcct ctaacgcagc
      181 tttgttttgc atttgtgccg ctaacgctgc tacggtgatg cctgtttgtt tctcaatttt
      241 ttctccataa ctattcgcaa tagtttcggt ttgcattgtt gcatccactt ttttttcagt
      301 tgatggattg caagcgaata gataagttag tgaaagtaaa acaataccct ttttcataat
      361 attcattttt ttaaagtaaa ccgcaaaagt acagtattcc gatttttcaa gttacctaaa
      421 attcaatttt attttaaaca attttaaaga ataatttttg tgaatgggca aaatttaact
      481 gtgatttttt tgtgaaaaca tttttttata ttagcggctg tttatagcac acacaccagt
      541 ttaccttttt accaatttac caatgcatta ttttatgaaa aaaataatag ctacatgcat
      601 ggctattatt atttgtgcaa catttttgca cggacaaatt aatttgcaac agccaaattt
      661 tgttgactat cttatatcct caggttggaa tcaacctgcc ggtattgtat atgattgcac
      721 aggccaatcc tatatatggg tacttaaagg caaaatttat gtagttgatt cagccggaaa
      781 taaaataaca acaccactgc tcaatattgc tgaggaagta ggcaattggc gttcgcatgg
      841 tttgcatggc gtttgtcttg accctaactt tagaacaaat ggttattttt attgctatta
      901 tgctgttgac cgttatcact tattaaattt tggtaaacca aattataatg caaactcgaa
      961 tgcatactat gatgcatcta ttttacgcgt aaccagattt acggccaata gtgcaacaaa
     1021 ctttacaacg ttagtcccaa attctcgttt ggtactgttg ggtgaggaca aaaaaacagg
     1081 tgtgccctta ctacatgtta cacatgatgg aggtactatt ttgtttggcc gcgatggtag
     1141 tttgctggta tttactggcg attgtgcaga ttatgctcaa aacgatgtag gcagtgcacc
     1201 gggtacttat tgggcacagg cattggccga taccattatc agaactgcag aaaatgtggg
     1261 cgatttcagg tctcagttat
//
LOCUS       DAOMAA010000788         1279 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_134917_length_1279_cov_2.410948, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000788 DAOMAA010000000
VERSION     DAOMAA010000788.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134917_length_1279_cov_2.410948"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(189..>1279)
                     /locus_tag="PLO59_09720"
     CDS             complement(189..>1279)
                     /locus_tag="PLO59_09720"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HQV01426.1"
                     /translation="SLKVNHNSKFIIASVSKIFIKYALLKLVELNKLELTDSVSKYIP
                     DFPNGSKITIEHLVKHQSGLPRELSGNHEIDTLSLAKIIAIAKTEKLQFEPGTQTLYS
                     NVGYFLLHYIIEKTASDGYAAFMHDAIFKPMGLNNTTELHALPANMPFVYGFDNVDGT
                     ITATSKANLYQTETGNYLSTISDLHAFSKSILNEKNLPNNLALKIFGSDSLLVQAGGR
                     PGYRAYFYLNPKTKTTFIFACNYSDIPMQKITTDVINMLAGKPYEMPVNINRKAIDVP
                     LRLLQKYAGNYVLEADISKIITIKFIGNKLYNVDDTEQTELFADSANTFFENPNSNDG
                     YVFKYNPQTQKYDLTIVFNGMPLKTKRQ"
BASE COUNT          390 a          187 c          244 g          458 t
ORIGIN      
        1 gtaattgaaa aaattgcttt cgatggttct atgattattc aaataggtaa ggctaacaga
       61 acaactgtat caaaaaagtt tgccgaaaat ttatttgtag cataatccac ctggtgtgta
      121 cttactttat gaagctgaca attttattgg cttaagtatt gtaagctgaa aaaataatat
      181 ccctgaaatt attgccgttt ggtttttaat ggcatgccat taaaaactat agtcaaatca
      241 tatttttggg tttgcggatt atatttaaaa acgtatccat cattactgtt tggattttcg
      301 aagaaggtat ttgcactatc agcaaataat tcggtttgct cagtatcatc aacattataa
      361 agtttattgc caataaattt gattgtaatg atttttgaaa tgtcggcttc taacacataa
      421 ttgccggcat atttttgaag caatcttaat ggcacatcta tagctttgcg atttatgttt
      481 acaggcattt cataaggttt accggcaagc atgtttatta catcggttgt aattttttgc
      541 atgggtatat cggagtaatt gcaggcgaaa ataaaggtag tttttgtttt agggttcaaa
      601 taaaaataag cgcggtagcc gggtctgcca ccggcctgaa ccagtaaact atcactgcca
      661 aatattttta atgcaaggtt gttaggcaag tttttttcat ttaaaattga tttgctaaat
      721 gcatgcaaat cggaaattgt ggatagataa tttccggttt cggtttgata caaatttgct
      781 tttgatgtag ctgtaatcgt accatcaaca ttatcgaaac cataaacaaa aggcatgttt
      841 gcaggcaatg catgtaactc tgttgtgttg tttaaaccca tgggtttaaa aattgcatca
      901 tgcataaatg ctgcataacc atcggatgca gttttttcga ttatataatg caatagaaaa
      961 tagccaacat ttgaatataa tgtttgggta ccgggttcga actgcaattt ttcggttttt
     1021 gctatcgcaa ttattttagc gagggataac gtatcaattt catggttgcc tgataattcg
     1081 cgtggcaggc ccgattggtg ctttactaaa tgttcaatgg ttatttttga tccattcgga
     1141 aaatctggaa tatattttga aacagaatcg gttagttcca gtttatttaa ttcaacaagt
     1201 tttagcaggg cgtatttgat aaatattttc gaaaccgatg caataatgaa tttgctgttg
     1261 tgattaactt ttaggctat
//
LOCUS       DAOMAA010000789         1277 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_135328_length_1277_cov_2.690671, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000789 DAOMAA010000000
VERSION     DAOMAA010000789.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135328_length_1277_cov_2.690671"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="PLO59_09725"
     CDS             complement(<1..704)
                     /locus_tag="PLO59_09725"
                     /inference="COORDINATES: protein motif:HMM:NF018899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HQV01427.1"
                     /translation="MIKYIAGILIFIGCNFTNAQTAVSDTQKVVVSRIVTIGNKVTRQ
                     SIINRELIFHVGDTLPQFVLNSAMVRSKENLMNTFLFNFVEIDAINDADNKKQIIVKV
                     TERWYLFPVPIFELADRNFNEWWQTKDFSRTIYGFYVTHQNFRGRKELLQLSARFGYA
                     QKFNLNYVLPYINKKQNQGLQFTGGYNRNHEVSYSLSASVFAFYTNDEKFIRKEWFAA
                     AKYTLRNGIYKYTNLAA"
     gene            complement(717..>1277)
                     /locus_tag="PLO59_09730"
     CDS             complement(717..>1277)
                     /locus_tag="PLO59_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HQV01428.1"
                     /translation="MLPLISHKKYLGLINKKLLIESDLQMPLQQLHHDLTHPYLIESQ
                     HIYDVFAYAANHHFSLLPVLNQKQEYVGLITKQDIIASFASFKSLQVPGSIVVLQVPL
                     KDYQLSHIIQIIESNDANALNVDVQILPDFEIAEVTIKINRIDLSRIESAFYRHNYVI
                     KSLYHKSQYADELNSRYDSLMNYLNM"
BASE COUNT          377 a          236 c          236 g          428 t
ORIGIN      
        1 gcagctaagt ttgtatattt ataaatgcca ttgcgcaacg tgtatttagc ggcagcaaac
       61 cactctttac gaataaattt ttcgtcattg gtgtaaaagg caaatacgct ggcgcttaaa
      121 ctatacgata cctcgtgatt acggttataa ccacccgtaa attgtaatcc ctggttttgt
      181 tttttgttga tgtatggcag cacatagttt aaattaaatt tttgcgcata cccaaagcgt
      241 gcacttagct gaagcaattc tttgcgacct ctgaaatttt gatgcgttac ataaaaacca
      301 taaattgttc ggctaaaatc tttggtttgc caccactcat taaaatttct atcggctaat
      361 tcaaaaatgg gtaccggaaa caagtaccaa cgctcggtaa cttttactat tatctgtttt
      421 ttattatcgg catcgtttat ggcgtctatt tctacaaagt tgaacaggaa ggtgttcatc
      481 agattttctt tgctgcgcac catagcgctg tttaaaacaa actgtggcaa tgtatcgccc
      541 acatgaaaaa ttaactcgcg gttaataatg gattgccggg ttactttatt accaatggtt
      601 acaatacggc ttactactac tttttgagta tcggataccg ctgtttgcgc attagtgaag
      661 ttacaaccaa taaaaattaa aatgccggct atgtatttta tcatccattg cgtagcttac
      721 atattcagat aattcataag cgaatcgtac cggctgttta actcatcggc atactggctt
      781 ttatgataca gacttttaat aacatagttg tggcggtaaa atgcactttc gatacggctt
      841 aaatcaatac ggttaatttt tatggtaacc tctgctattt caaaatcggg taatatttgc
      901 acatccacat taagggcatt ggcatcgttg ctttcaataa tctgtataat gtgcgacaac
      961 tgataatcct ttagtggaac ttgtaacact acaatactgc ccggtacttg caatgattta
     1021 aatgatgcaa aactggctat tatatcttgt ttggtaataa gacctacata ctcctgtttt
     1081 tggttaagta caggcagcaa cgaaaaatgg tggtttgcag cgtaggcaaa cacatcataa
     1141 atatgctgac tttcgattag gtatggatgc gttaaatcat ggtggagttg ttgcaatggc
     1201 atttgcaggt cactttcaat caatagcttt ttgtttatca agcctaaata ctttttatga
     1261 ctaattaaag gcagcat
//
LOCUS       DAOMAA010000790         1275 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_135678_length_1275_cov_3.986885, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000790 DAOMAA010000000
VERSION     DAOMAA010000790.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135678_length_1275_cov_3.986885"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="PLO59_09735"
     CDS             complement(<1..753)
                     /locus_tag="PLO59_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016775819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HQV01429.1"
                     /translation="MKKEKVKVLFISQEITPFVESTEISKICRHLPQGTQESGKEIRV
                     FMPRYGLVNERRNQLHEVIRLSGMNLIINDLDHPLIIKVASIQQARMQVYFIDNEEYF
                     QRKAMVTDAKGKYFKDNDDRTVFFCRGVLETVKKLGWVPDIIHCHGWITSLIPMFVKT
                     AYKEDPCFKNARVVYSVYNDAFEGNWPANYNKKISMEGISANDIKELKDANYTNLSLN
                     AIRHSDALIRGSETISKEIETAMKKSGKNTLPL"
     gene            complement(950..>1275)
                     /locus_tag="PLO59_09740"
     CDS             complement(950..>1275)
                     /locus_tag="PLO59_09740"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV01430.1"
                     /translation="VRVFDNAGCSHMSNYLPVAGCAGVNNINSSISVLYPNPASDFIN
                     VIFNKPLTQHAWYKIIDVWGRALVTETNLTSNNYLTIKTPFASGVYQLIVWNNGYSAI
                     NFIKR"
BASE COUNT          406 a          239 c          206 g          424 t
ORIGIN      
        1 taatggcaaa gtgtttttgc ctgatttttt catggcagtt tcaatctctt tagaaatggt
       61 ttcgctaccg cgtatcaatg catcagaatg gcgtatggca tttaaactta ggtttgtata
      121 atttgcgtct ttaagctctt taatgtcatt ggccgaaata ccctccatac tgattttctt
      181 attgtagtta gcaggccagt ttccttcaaa agcatcatta taaacactgt aaactacacg
      241 tgcattttta aagcaagggt cttctttata agccgttttt acaaacatgg gtattaatga
      301 ggttatccaa ccatggcagt gaattatatc aggcacccaa cctaattttt taacggtttc
      361 taatacacca cgacagaaaa atacagttct atcgtcatta tccttaaagt attttccttt
      421 ggcatcggta accatggcct tacgttgaaa atactcttca ttatcaataa aatatacctg
      481 catgcgcgct tgttgtatgg aggcaacttt aataattagc ggatgatcaa ggtcatttat
      541 aataaggttc ataccgctta accgtatcac ttcatgtaat tggtttcgcc tttcatttac
      601 caaaccgtaa cgtggcataa aaactctgat ttctttacca gattcctgtg tgccctgagg
      661 caggtgacgg caaatttttg aaatttcagt tgattcaaca aaaggagtta tttcctgaga
      721 tatgaaaaga actttaactt tctctttctt cattactatc gcgtttttaa aatgaggcac
      781 aaaagtaagc aactttaaaa aaactcacaa ccaaaagccc tttgtttact acgtattaac
      841 caaaataaat gtcttgtttt tttaaaatat attattaata aaagcaagta cttacagcat
      901 aatataattc agtaggggct atcggaaaag taactttaca aacattttac taccttttaa
      961 taaagttaat agcactataa ccattattcc aaacaatcaa ttgataaaca cctgaggcaa
     1021 atggtgtttt tatggttaag tagttgttgc tggttaagtt agtttcagta acaagtgctc
     1081 taccccatac atcaattatt ttataccaag catgctgtgt caatggttta ttaaatatta
     1141 cattaataaa atcgctggct gggttggggt acaaaacaga tatagacgaa tttatgttgt
     1201 taactccggc acaaccggca accggcaaat aattgctcat gtggctacaa ccggcattat
     1261 caaaaacccg cacac
//
LOCUS       DAOMAA010000791         1275 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_135760_length_1275_cov_2.469672, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000791 DAOMAA010000000
VERSION     DAOMAA010000791.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135760_length_1275_cov_2.469672"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            106..>1275
                     /locus_tag="PLO59_09745"
     CDS             106..>1275
                     /locus_tag="PLO59_09745"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HQV01431.1"
                     /translation="MAIIGSIRNRMGVLIIVFVGFALVAFILGDFLTTNRSFLGNDNS
                     LATIAGESINPQEFDAKVREAEDLMKMQQNLENLTAEQSDMARNQAWQQLLNDKIYGA
                     QIEKLNLVVTDDEVNDMVFGKFLHPGISGEKAFQNPATGQFDPVKVTQYLKQLENPGS
                     NPEVAKAKVQWLNFEDGIRKERLLSKYSDLITKAIYVTNAEAKADYENKGTVYNVKAI
                     NLNYASIADSTVKVSDGDLKSYYESHKWEFKQPETAASISYVSFDVNPSEKDISDART
                     YIAQLVEPFKTATNDSVFINVNADTKYTGTYMARGSFSPAIDSVVFRTNVGTVIGPYD
                     EAGSVKIAKVLGFKTIPDSVRARHILITPEQGQDPAIAQKLADSLNTVLKAGGDWM"
BASE COUNT          418 a          240 c          263 g          354 t
ORIGIN      
        1 gtatttaatc agccttacaa gccatatcaa atatgggctt gcctttgtaa aacacttgta
       61 tatatttgca ccccttattt taaaaaaatc aatacataat cagtaatggc aatcattgga
      121 agtatcagaa accgcatggg tgtactcatc atcgtatttg tgggttttgc attagtagct
      181 tttatcctgg gcgatttttt aaccaccaac aggagttttt taggtaacga taatagtctg
      241 gcaaccattg ccggtgaaag cataaatccg caagagtttg atgccaaagt acgcgaggct
      301 gaggatttga tgaaaatgca acaaaacctc gaaaacctta ccgctgaaca atcggatatg
      361 gctcgtaatc aggcatggca acagttgctg aatgataaaa tttatggagc gcaaattgaa
      421 aaactaaact tagttgtaac ggatgacgaa gttaacgaca tggtttttgg caaattttta
      481 catccgggta tcagtggcga aaaagccttt caaaatccgg caaccggtca gttcgatcct
      541 gttaaagtta cccaatactt aaagcaatta gaaaacccgg gcagtaaccc cgaagttgcc
      601 aaagctaaag tacaatggtt aaattttgaa gatggaattc gtaaggaacg cctgcttagt
      661 aaatacagcg atttaattac caaagccatt tatgttacca atgccgaagc taaagccgat
      721 tatgaaaaca aaggaactgt ttataatgta aaggcaataa atttaaacta tgcctccatt
      781 gccgatagca ctgtaaaagt aagcgatggt gatttaaaaa gttattacga atcgcataag
      841 tgggagttta agcaacccga aactgctgca agtattagtt acgtttcgtt tgatgtaaac
      901 ccaagcgaaa aggatataag tgatgcgcgt acctatattg cacaattggt tgaaccattt
      961 aaaactgcta ccaacgattc tgtttttata aatgtaaatg ccgatacaaa atacactggt
     1021 acgtacatgg cacgcggcag cttttcgcca gctattgact ctgtggtgtt tcgtaccaat
     1081 gtaggcaccg taattggccc ttacgatgaa gcaggctctg taaaaattgc taaggtttta
     1141 ggttttaaaa caattccgga ttcagtgcgc gcacgtcata ttttaattac gcctgaacaa
     1201 ggtcaggatc cggcaattgc acaaaagtta gccgattcat taaacaccgt acttaaagcc
     1261 ggtggcgatt ggatg
//
LOCUS       DAOMAA010000792         1275 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_135812_length_1275_cov_1.965574, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000792 DAOMAA010000000
VERSION     DAOMAA010000792.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135812_length_1275_cov_1.965574"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="PLO59_09750"
     CDS             <1..493
                     /locus_tag="PLO59_09750"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV01432.1"
                     /translation="NPVAHFMYPGITDPAFPGINWTEGSAGNAPEDRRMMGSIGPMAF
                     DQGYVISVDVATLFVNGGQPAMRTAAAYVDSIFQNNIALQVPVVLAEPQLNLFPNPAV
                     NSFKITGNAERINIINAQGVIVQTVDNYQQQFINIQHLPSGVYAVQCINGAKINTIRL
                     CKR"
     gene            complement(550..>1275)
                     /locus_tag="PLO59_09755"
     CDS             complement(550..>1275)
                     /locus_tag="PLO59_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01433.1"
                     /translation="NLHRYAYQVNGNLNVYDKTENVIAGIARKAHQTTGTTHSVMVID
                     EDKFSGSILDKKGNYNYYTAQLFNDIFFENTPVKLIESKTAGKRNKHIEQLKTLLFSP
                     GTSVKGVPLVGNKLQVFDTQQKYYDYFLNTAKYNDSLVCYVFTCTAKTNATGQSNGNA
                     IIKNLVTYFDKKTFQVIARKYEMQYASVAFDFDVTMDIKISPVNGVFVPTYISYMGNW
                     DVAFKKEEKADFQLKFFNWIIRP"
BASE COUNT          380 a          236 c          230 g          429 t
ORIGIN      
        1 taatccggtg gcacatttca tgtatccggg cattaccgac cctgcatttc cgggaattaa
       61 ctggactgaa ggctcggcag gtaatgcgcc tgaagataga aggatgatgg gcagtattgg
      121 ccccatggca tttgaccaag gatatgttat atctgtggat gtagctacat tgtttgtaaa
      181 tggtgggcaa cccgcaatgc gcactgcagc agcttatgtt gattccattt ttcagaacaa
      241 tattgctttg caggtacctg ttgtattagc agaacctcag ttaaatttgt ttccaaaccc
      301 tgcagtaaac tcgtttaaaa ttaccggcaa tgccgaaaga attaatataa taaatgcgca
      361 aggtgtcatt gttcaaactg ttgataacta ccaacaacag tttattaata tccagcattt
      421 accatcgggc gtttatgcag tgcagtgtat taatggtgct aagattaaca ccattagatt
      481 atgtaaaaga taaattgaaa ttataaaaca agagcaggta acttacgcgg ttacctgctc
      541 ttgtcttatt tagggcctta taatccaatt aaagaatttt aattgaaagt cggctttctc
      601 ttctttttta aaagccacat cccaattgcc catgtatgaa atataggttg gaacaaaaac
      661 gccattaacg ggacttattt ttatatccat agtaacatca aaatcaaaag caacacttgc
      721 atattgcatt tcatatttac gtgcaattac ctgaaacgtt tttttatcga agtacgtaac
      781 cagatttttt attatggcat ttccattact ttgaccggtt gcattggttt ttgctgtgca
      841 tgtaaaaaca taacatacta aagagtcgtt atattttgct gtgtttaaaa agtaatcgta
      901 atatttttgt tgcgtatcaa aaacttgcaa cttgttgcca accaacggta caccctttac
      961 cgaagtaccc ggactaaaca aaagggtctt taattgttcg atgtgtttat ttctttttcc
     1021 ggcagtttta ctttcaatta attttaccgg tgtattttca aaaaatatat cattaaacaa
     1081 ttgtgcagta tagtagttat agttgccttt tttatccagt atgctaccac taaatttatc
     1141 ctcatcaata accattacac tatgcgtagt tccggtggtt tgatgtgctt tacgcgcaat
     1201 gccggcaata acattttcgg ttttatcata cacatttaag ttgccattaa cctggtatgc
     1261 atagcgatgc aggtt
//
LOCUS       DAOMAA010000793         1274 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_136003_length_1274_cov_2.129614, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000793 DAOMAA010000000
VERSION     DAOMAA010000793.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136003_length_1274_cov_2.129614"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1226)
                     /locus_tag="PLO59_09760"
     CDS             complement(<1..1226)
                     /locus_tag="PLO59_09760"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HQV01434.1"
                     /translation="MSYLPASVFFNQDVKYLKGAGPQRAELLQSEYGIKTYGDLILHF
                     PYRWVDRSRFYKINELTEEAPFVQLRGKIKTISTAGDKRATRLIATLSDDTGSIDLVW
                     FQQIKYLRETLKAGSDYVVFGKPTAFNGKINLVHPDVELLATFNETLSGNMQSMYPTT
                     EKSKGRGLDSKAFWRMQKNLIAQLPPELDECLPTTIINQTKLISLRQALIQIHFPDDE
                     RSLITARYRLKFDELFYLQIQLLQQKLVRTHKSRGRVFSKVGDLFNTFYKQHLPFDLT
                     QAQKRVLKEIRQNTLTGHQMNRLLQGDVGSGKTIVALMCMLLCIDNGMQACIMAPTEI
                     LAQQHYTSITALLRNMPVSIALLTGSTKSKERKHILEALENNDLNLIIGTHALIEDQV
                     VFADLGLAVIDEQHRFG"
BASE COUNT          367 a          250 c          226 g          431 t
ORIGIN      
        1 ccaaaacgat gttgctcatc aattacagct aaacccaaat cggcaaaaac aacctgatct
       61 tcaattaatg catgtgtacc aattattaag tttaaatcat tgttttccaa tgcttctaaa
      121 atgtgtttac gctctttgct tttggtacta cctgtgagca atgcaataga aacaggcatg
      181 tttcttaata aagccgtaat acttgtataa tgttgttgtg ctaaaatttc ggtaggcgcc
      241 ataatgcaag cctgcatgcc attatcgatg cataacaaca tacacataag tgctactatg
      301 gttttaccac tgcctacatc gccttgcaaa agcctgttca tttgatggcc tgttaatgta
      361 ttttgtctga tttcttttag tacacgtttt tgtgcctggg ttaaatcgaa cggcagatgt
      421 tgtttataaa aagtattaaa caaatcgccc actttcgaaa acacccttcc tctgctttta
      481 tgtgtacgca ctaacttttg ttgcagcagt tgtatttgca agtaaaacaa ctcatcaaat
      541 tttaaacggt aacgcgctgt tattaacgat ctttcatcat caggaaaatg tatttgaatt
      601 aatgcctgcc gtagcgaaat taactttgtt tgatttatta ttgttgtagg caaacactcg
      661 tcaagttcgg gtggtagttg ggcaattaaa tttttttgca tgcgccaaaa tgctttactg
      721 tctaatccac gtcctttgct tttttctgtt gttggataca tgctttgcat gttgccactt
      781 aaggtttcat taaaagtggc cagcaattct acatcgggat gcaccaaatt aattttgcca
      841 ttaaaggcag ttggttttcc aaacaccacg taatcagaac cggcttttaa tgtttcgcgc
      901 aggtatttta tttgttgaaa ccacaccaaa tcaatgctgc ctgtatcgtc acttaatgtt
      961 gcaattaatc gtgtagcacg tttgtcgcct gcagttgata tggttttgat tttacctcgt
     1021 aattgtacaa aaggagcttc ttcggtaagt tcgttaattt tatagaatct gctccgatca
     1081 acccaccggt aagggaaatg cagaattaaa tcgccataag ttttaatgcc atattcggac
     1141 tgtaacaact cagcacgttg tggcccggca cctttaaggt actttacatc ctgattaaaa
     1201 aaaactgatg cgggcaggta actcatttcg caaataaaat gatatcataa ttatgattaa
     1261 acataattgc tact
//
LOCUS       DAOMAA010000794         1267 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_137386_length_1267_cov_2.822607, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000794 DAOMAA010000000
VERSION     DAOMAA010000794.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137386_length_1267_cov_2.822607"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1267
                     /locus_tag="PLO59_09765"
     CDS             <1..>1267
                     /locus_tag="PLO59_09765"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HQV01435.1"
                     /translation="WMQFDTLRGSTPDMMQQMVSTRVSTIDNIEAKYPLIISNFYGHF
                     LVLIDSNFEANYMVLLHGNQIENLVYKVLVDKNQNIWCAGATYGLHSINKIEKHEVDL
                     PPKLFGALLGSYNPFHLNTNDLSFDKHAYLFAPGKPYILYNADQQYNVLDILPFSDST
                     YIVSIMGNGLHYFNPNTAKHYTSIKSDQKTFRGLQLDANHNIWAISGNYLVRYNTTKK
                     TWLHIDVNDGLPASGLNNTMYNDKLFFYAGGMGYYIKFNPLSIDSSLTRQPLYLSGLQ
                     LNNIIADSLLYDKEPHISQKFNSINFQYAYLEFTNPKAINYRYKLEGLNQEWINAGNE
                     NHVVFSNLGSGNYQFKVAAYYKDNETMLAQASYSFYIEPFWYNTWWFKISIACLLVLS
                     GYGIYRYRLSQLHKFYNIRNNIARNLHDEV"
BASE COUNT          428 a          231 c          217 g          391 t
ORIGIN      
        1 cgtggatgca gtttgacacc ttgcgtggca gcacacctga catgatgcag caaatggttt
       61 cgacacgggt ttctactatt gataatattg aagcaaaata ccctttaata atcagcaatt
      121 tttatgggca ctttttggtt ttaattgata gtaattttga agccaactat atggtgcttt
      181 tacatggcaa tcaaattgaa aacctggttt ataaagtgtt ggttgacaaa aatcaaaata
      241 tttggtgtgc cggtgcaact tatggattgc acagtatcaa caaaatagaa aaacatgaag
      301 ttgatctccc acccaaatta tttggtgctt tgttaggcag ctataaccca tttcatctta
      361 atacaaacga tttatcattt gataaacacg cctatttatt tgcgcccggc aaaccctata
      421 tactttacaa tgcagaccag caatataatg tgcttgacat tttgccattt agcgatagca
      481 catatattgt ttccataatg ggcaatgggt tacattactt taatccaaat acagctaaac
      541 attatacatc catcaaatct gatcaaaaaa cattcagagg cctgcagttg gatgcaaacc
      601 ataatatatg ggcaataagt ggcaattatt tagtgcgata caatacaact aaaaaaacct
      661 ggctacacat tgatgttaat gatggattgc cggcatcggg tttaaacaac accatgtata
      721 acgataaact gtttttttat gccggaggta tgggttacta tattaaattt aatcccttgt
      781 ctatcgactc ttcactaaca cggcagccac tatacctttc gggtttacaa cttaacaaca
      841 tcattgccga tagtttactt tatgacaaag aaccacacat cagccaaaaa tttaacagca
      901 tcaattttca atatgcctat ctggaattta caaacccaaa agccataaac tatcgctaca
      961 aattagaagg attaaatcag gaatggataa atgctggcaa cgaaaatcat gtggtgttta
     1021 gcaatttagg atcgggtaat tatcaattta aagtagctgc ttattataaa gacaatgaaa
     1081 ccatgttggc gcaagcaagc tatagttttt atatagagcc cttttggtat aacacgtggt
     1141 ggtttaaaat atcaattgca tgcttgttgg ttttatcagg atatggcata taccgatacc
     1201 gcctcagtca attgcataaa ttttataata tcagaaataa catagcacgc aatttacatg
     1261 atgaagt
//
LOCUS       DAOMAA010000795         1265 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_137956_length_1265_cov_1.247107, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000795 DAOMAA010000000
VERSION     DAOMAA010000795.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137956_length_1265_cov_1.247107"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1265)
                     /locus_tag="PLO59_09770"
     CDS             complement(<1..>1265)
                     /locus_tag="PLO59_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01436.1"
                     /translation="PRYLSAFDFKGSIAVSVGSAGRMYRSSDGGDNWTGVNSNTFEDL
                     LDVTFLQGNNVISCGDGVVSRSSDNGLTWAADTSFNNDFIYALSKAPNSNTVFMNSSS
                     IVYKSTNGGTTFNSYAFPFSNMQHIFAINNDTVLVGTGAGVYISYDGALHWQQFATTQ
                     NFIVNKVAYNAPYMYALCDSSTLLRADINTTPAEPFASFDYIEDNLCGVTHVTCTNNS
                     DPGYTMAWYLNGAFVSSSYNYNIDYTVTTPNQEIKLIVTSPSGTDTAIVKNSIYVKKL
                     AVAIAQPDEYNCYGQEMILSGKDTLGQYIAWLPQSKLNLLYFGLNADSVLTKPILQQQ
                     DTIIFQTKNNDNCIALDTVFIFTTPPAFEPFKKVVPGLKDFCSNAPCNFISGLDFINN
                     QEGFGFANTTEFLKTTDGGATYSKLFLQP"
BASE COUNT          409 a          239 c          228 g          389 t
ORIGIN      
        1 aaaggctgca aaaaaagttt tgaataagta gcgccaccat cagtggtttt taaaaattct
       61 gttgtgttgg caaaaccaaa accttcctgg ttatttataa aatccaatcc cgaaataaaa
      121 ttacatggcg cattgctgca gaaatctttt aagcccggta ctactttttt gaaaggttcg
      181 aaagccggtg gagtagtaaa aataaaaaca gtatcgagcg caatgcagtt atcattgttt
      241 tttgtttgaa agattatggt atcctgttgt tgcaatattg gcttcgtaag aacgctatct
      301 gcatttaatc caaaatataa aagattcaat ttgctttgcg gtaaccatgc aatgtattgc
      361 ccaagggtat ccttgccaga taaaatcatt tcttgtccgt agcaattgta ttcatcgggc
      421 tgtgcaattg ctacagctaa ttttttcaca taaatactat ttttaactat agctgtatcc
      481 gtacccgatg gtgatgttac tataagttta atttcctgat tgggtgtagt aactgtataa
      541 tcaatattgt aattgtagct gctgcttaca aaagcaccat ttaggtacca ggccattgta
      601 taaccagggt ccgaattatt tgtacaagta acatgcgtta cgccacacaa attatcttca
      661 atataatcga acgatgcaaa aggctcagca ggcgtggtat ttatatcggc acgcaataaa
      721 gtactgctat cgcataatgc atacatgtat ggagcattgt atgcaacttt atttacaata
      781 aaattttgtg ttgtggcgaa ttgttgccaa tgcaatgcac catcatacga aatgtaaacg
      841 cctgctcctg taccaactaa aacagtatcg ttatttattg caaaaatatg ttgcatgttc
      901 gaaaatggaa atgcgtatga attaaaagta gtaccaccat tagttgattt ataaacaatg
      961 gaagatgaat tcataaaaac agtattcgaa ttgggtgcct tactcaatgc ataaataaaa
     1021 tcgttgttga atgatgtgtc agcagcccat gttaagccat tgtcgctcga acgcgataca
     1081 acaccatcgc cacatgaaat tacgttattg ccttgcaaaa atgttacatc aagcaaatct
     1141 tcgaaagtgt tgctgtttac acctgtccag ttatcgccac catcactgct acgatacatc
     1201 ctacctgccg aaccaacact tacggcaata gatcctttaa aatcgaaagc acttaaataa
     1261 cgtgg
//
LOCUS       DAOMAA010000796         1263 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_138219_length_1263_cov_3.255795, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000796 DAOMAA010000000
VERSION     DAOMAA010000796.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138219_length_1263_cov_3.255795"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="PLO59_09775"
     CDS             <1..231
                     /locus_tag="PLO59_09775"
                     /inference="COORDINATES: protein motif:HMM:NF017394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HQV01437.1"
                     /translation="TGELKATEKIKTLLISDYNTQQLIEVENAIYLNSETLHTPMNGQ
                     VAAFANQTEAEKVNLEFPGKLIKWNEVQQLFK"
     gene            232..>1263
                     /gene="nosD"
                     /locus_tag="PLO59_09780"
     CDS             232..>1263
                     /gene="nosD"
                     /locus_tag="PLO59_09780"
                     /inference="COORDINATES: protein motif:HMM:TIGR04247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071941 - nitrogen cycle metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HQV01438.1"
                     /translation="MMKSLLALCCFVLLLKCADASIINVGKSHKTQTIKKAIELAQPF
                     DTLQIENGIYKEGNLIVAKPLTLLGQKQTILDGENKHEILTISGSYFRIKGITFQNSG
                     YSSMNDFASIKIIDAHDFIIEQNRIINAHFAIHVSNSNRFVIKNNHITGTPAEEQSTG
                     NGIHLWKCSKALIANNNTIGHRDGIYFEFVTESEVLNNYSEKNIRYGLHFMFSNNDKY
                     ISNTFHDNGAGVAVMFSHHIAMHYNKFTLNRGGSAYGILLKEISDGEVYANTFSNNTV
                     GVFMEGTNRIHFKHNKFYSNGYAMKVQASCNENNIENNNFTGNTFDVATNGSLVLNTL
                     SKNYWDKYEG"
BASE COUNT          474 a          227 c          217 g          345 t
ORIGIN      
        1 accggagagc taaaagcaac cgaaaaaatt aaaaccttat taatatctga ttataacaca
       61 cagcaactta tcgaagttga aaatgctatt tacctcaaca gcgaaacatt gcacacaccc
      121 atgaacgggc aagtagcagc atttgccaac caaaccgaag ccgaaaaagt aaatctcgaa
      181 tttccgggaa aattaataaa gtggaatgag gtgcagcagc tttttaaata aatgatgaaa
      241 tcgttattag ctttatgctg ctttgtactg ctattaaaat gtgctgatgc ttccattata
      301 aacgtaggaa aatcgcataa aacacaaacc ataaaaaaag cgattgaact ggcacaacct
      361 tttgataccc ttcaaattga aaacggaatt tataaggaag gaaatctgat agttgccaaa
      421 cccttaacac tgcttggtca aaaacaaacc atattagatg gtgaaaataa acatgagatt
      481 ttgaccataa gcggcagtta ctttcgtata aaaggcatta catttcaaaa ctcaggctac
      541 tcatctatga atgattttgc ttcgattaaa attattgatg cccacgattt tattatcgaa
      601 caaaaccgca ttataaatgc acattttgcc atacacgttt caaatagcaa tcgtttcgta
      661 ataaaaaaca atcatattac aggtacacct gccgaagagc aatcaaccgg aaatggcatt
      721 catttatgga aatgctcaaa agccctaatc gcaaataata atacgattgg tcatcgcgat
      781 ggtatttact tcgaatttgt aaccgaatcg gaagtattaa ataactacag cgaaaaaaat
      841 atccgttacg gtttgcattt tatgttttcg aacaacgata aatatatttc aaacacattt
      901 catgataatg gggccggtgt agccgttatg ttttcgcatc atattgccat gcactataat
      961 aaatttacat tgaacagagg cgggtcggca tacggaattt tattaaaaga aatatcagat
     1021 ggcgaagttt atgcaaatac attcagcaac aatacagtag gtgtttttat ggaaggcacc
     1081 aaccgcatac attttaaaca taataaattt tacagtaacg gatatgccat gaaagtgcag
     1141 gcaagctgca atgaaaacaa tattgaaaac aacaatttta caggcaatac ttttgatgtg
     1201 gcaactaacg gatcgctggt gcttaataca ctttcaaaaa attactggga taaatacgaa
     1261 ggg
//
LOCUS       DAOMAA010000797         1262 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_138405_length_1262_cov_3.815244, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000797 DAOMAA010000000
VERSION     DAOMAA010000797.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138405_length_1262_cov_3.815244"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1262)
                     /locus_tag="PLO59_09785"
     CDS             complement(<1..>1262)
                     /locus_tag="PLO59_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008240697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HQV01439.1"
                     /translation="RERIDYLLDKDKPRIEIGAFVADGMYVEHGGCCSGGVVVMIGYI
                     AGVQCIVVANDATVKAGAWFPLTAKKNLRAQEISIENKLPIVYLVDSAGVYLPMQDEI
                     FPDKEHFGRMFRNNAVMSAMGIIQIAAIMGSCVAGGAYLPIMSDEAMIVDKTGSIFLA
                     GSYLVKAAIGEDIDNETLGGATTQCEISGVTDNKYKDDESALDYIRSIFSKLGANPKA
                     GFNRVESKLPVNDVNDVYHIIPNTRDKQYDMHQIINCLLDNSEFDEYKEKYGQSIICG
                     IGRIDGWAVGIVANQRTVVKNKKGEMQFGGVIYSDSADKAARFIMNCNQRKIPLVFLQ
                     DVTGFMVGSKSEHGGIIKDGAKLVSAMSNAVVPKFTIIIGNSYGAGNYAMCGKAYDPR
                     LIVGWPTARIAVMGGAQAAKTLLQITTST"
BASE COUNT          353 a          302 c          212 g          395 t
ORIGIN      
        1 gtggaggtag taatttgcaa aagtgttttt gctgcctgtg caccgcccat tacagcaata
       61 cgtgcagttg gccaaccaac aatcaaacgt gggtcgtatg ctttgccaca catagcataa
      121 ttgcctgcac cataactgtt gccaattatg atggtaaatt taggcactac ggcattactc
      181 attgcactta ctaatttagc tccatcttta ataatgccac catgctcgct tttactgcct
      241 accataaagc ctgttacatc ttgtaaaaaa accaatggta ttttgcgttg attgcaattc
      301 attataaagc gtgcagcttt atcggcacta tcgctataaa taacgcctcc aaattgcatt
      361 tcgccttttt tgttttttac tacggtacgc tggttcgcta cgatgcccac agcccaacca
      421 tctatgcggc caataccgca aattatgctt tgtccatact tttctttgta ttcatcaaac
      481 tcgctgttat ctaataagca attaattatt tgatgcatgt cgtattgctt atcgcgtgta
      541 ttgggtatta tgtggtaaac gtcatttaca tcattaacgg gtagcttgct ttcaacccgg
      601 ttaaagcctg cttttgggtt tgcacctaat ttcgaaaata tgcttcgtat ataatctaaa
      661 gcactttcat cgtctttata tttattatcg gttacgccac taatttcgca ttgagtggtt
      721 gcgccaccta aggtttcatt atcaatatct tcgccaatag ctgcttttac caaataagaa
      781 ccggctaaaa atatggagcc tgttttatca acaatcattg cttcatcgct cataataggc
      841 aagtaagcac caccggcaac acagcttccc ataatggcag caatttgaat tatgcccata
      901 gcactcatta cggcattgtt tcgaaacatg cgtccaaagt gttccttatc cggaaaaatt
      961 tcgtcctgca taggtaaata cactccggca ctatctacta aataaacaat gggtaatttg
     1021 ttttcaatac ttatttcctg agcacgcaaa ttctttttag ctgttaacgg aaaccacgct
     1081 ccggctttta cagtggcatc gttggcaaca acaatacatt gcacaccagc tatataacct
     1141 atcataacta ctacgccacc actgcaacag ccaccatgtt caacatacat accatcggca
     1201 acaaaggccc caatttcaat acggggcttg tctttatcga gcaggtaatc aatacgctcg
     1261 cg
//
LOCUS       DAOMAA010000798         1260 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_138848_length_1260_cov_3.002490, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000798 DAOMAA010000000
VERSION     DAOMAA010000798.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138848_length_1260_cov_3.002490"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /gene="mraW"
                     /locus_tag="PLO59_09790"
     CDS             complement(<1..411)
                     /gene="mraW"
                     /locus_tag="PLO59_09790"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015812975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HQV01440.1"
                     /translation="MYHIPVMLSECLQALNIQGNENVVDVTFGGGGHSKAILQQLPNG
                     KLWAFDQDADAAANVLNDERFVLIPHNFKNLKQYLQLYGALPVDAILADLGVSSHQFD
                     TATRGFSTRFDAPLDMRMNKQGQETAASLLATLSA"
     gene            complement(413..877)
                     /gene="mraZ"
                     /locus_tag="PLO59_09795"
     CDS             complement(413..877)
                     /gene="mraZ"
                     /locus_tag="PLO59_09795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HQV01441.1"
                     /translation="MTQFLGEFDVTVDAKGRISFPVALRRQLSSEAQERFVMKKGFED
                     YIALYPMDEWKKVAAHISQLNPYIKKNRDFIRSFFKGVIEVELDSASRIQFPKRLLDA
                     AGIERDMVLFAHGNSIEVWSADKFEKMHQDMDADEMNALAEEVMGNFNQQPK"
BASE COUNT          379 a          297 c          198 g          386 t
ORIGIN      
        1 ggccgaaagt gtagcaagta aacttgctgc tgtttcctgg ccttgcttat tcatgcgcat
       61 atccagcggt gcatcaaaac gtgtactaaa gccgcgtgtt gctgtatcaa actggtgtga
      121 cgaaacgcct aagtcagcca gtattgcatc tacaggcaat gcaccataca actgcaaata
      181 ctgttttaag tttttaaagt tgtgcggtat caacacaaac cgctcatcgt ttaaaacatt
      241 ggcagcagca tcggcatcct gatcaaatgc ccatagttta ccatttggta attgttgcaa
      301 tatggcttta ctgtgtcctc caccaccaaa tgttacatcc accacatttt cgttgccttg
      361 tatgtttagc gcctgcaaac actcgcttaa cattacaggt atatggtaca tgttacttag
      421 gctgctggtt aaagttgccc atcacttcct cggccaatgc attcatctca tcggcatcca
      481 tatcctgatg catcttttca aacttatcgg cactccatac ttcaatactg ttgccatgag
      541 caaacaatac catatctctt tctataccgg ctgcatccaa caaacgtttc ggaaactgaa
      601 tacgacttgc actatcaagc tcaacctcaa ttacaccctt aaaaaatgaa cgaataaaat
      661 ctctgttttt tttgatgtat ggattaagct ggctgatatg tgcagctact ttcttccatt
      721 catccatagg atataaggct atataatctt caaaaccttt tttcatcaca aaccgctcct
      781 gagcctcaga cgacaattgc ctgcgcaatg ccaccggaaa cgatatacgt cccttagcat
      841 ccactgttac atcaaattcg cctaaaaact gagtcactgt tttttgttta atcggtttgg
      901 cccttcaaag cgcttcttac ctggctttgg gcatcctatg gggtcaaata tataaagcta
      961 atcacacaaa attctaatta attccacttt gttgataaat atatcccttt aacgcctcct
     1021 tttacaaaac attttacagt gcatgtaaaa acatctgcca aatttaaatg cacattaaac
     1081 ccaacaaaat aagctattta tgacaatttt acagtagcca ataatgtata ttattgccat
     1141 ttaacagaca taaaaacgcg gtggaattaa atgtaatttt ttcccaaaat tgtgaatatc
     1201 tgctacgttg cccaaaatag gttagaccac tactttatgc actttaaaaa cacagtaagg
//
LOCUS       DAOMAA010000799         1258 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_139292_length_1258_cov_2.471322, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000799 DAOMAA010000000
VERSION     DAOMAA010000799.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139292_length_1258_cov_2.471322"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="PLO59_09800"
     CDS             <1..260
                     /locus_tag="PLO59_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01442.1"
                     /translation="ILYIDDYTRRAIYFDRSLKLYCEDFVNETFDIYDCSLHAPEAAQ
                     FDFSTCYKQQLIVGVSGFDKEFIELSSWQVPYVESPSRITR"
     gene            578..>1258
                     /locus_tag="PLO59_09805"
     CDS             578..>1258
                     /locus_tag="PLO59_09805"
                     /inference="COORDINATES: protein motif:HMM:NF013698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HQV01443.1"
                     /translation="MEKEKQENGRFGLIYPNSAAVDVGSMEMYVSMPNAEGTSTVKVF
                     KAFTEDINGLVQELKQAQVTRVAMEATGVYWMAIFEMLEQAGLQVTLVNAHHFKNTDA
                     QKTDVKDCQWLHQLHEHGLLRASHVASESYRQLRTYLHERNVLQKQKSDGLNRIQRAL
                     TQMNLKIQHLISDIEGVSGMKIIRAIANGTQDPYQLLSEVNVKALKATEGDLIKSLTG
                     NYKEHHVQV"
BASE COUNT          418 a          227 c          262 g          351 t
ORIGIN      
        1 aaattttata cattgatgat tatacccgca gggcaattta tttcgacagg tcgcttaaat
       61 tatattgcga agattttgtg aacgaaacat ttgatatata cgattgtagt ttgcacgcac
      121 ccgaagctgc acagttcgat ttttctacct gctataaaca gcaattgatt gttggggtga
      181 gtggtttcga taaagaattt attgagctat catcgtggca agtgccttat gtggagtcac
      241 catcacgtat tacaaggtaa ttggttaata tgttattggt tgttgggttt tttgttctat
      301 gttcgtcatt gcgagtggcg aactcaaaac aggataacaa ataaatgatg acatttgctt
      361 tcgcataata tcatcgcagt aaactacaaa acctgttaca cactttggtg ccacacgagc
      421 ccgattgcga gtttaggact gttgtttact gaaaaaaata ttagtggtac cagtacgatt
      481 aaacactgtt ggtaaccctg cttgtaagct tagttatttt agagaacaac ggagttcatt
      541 gcgattaata ttttgcataa acctttaaaa aaaaattatg gaaaaggaaa aacaagagaa
      601 tggccgcttt ggacttatct atccaaactc ggcagccgtt gatgttggca gcatggaaat
      661 gtacgtatcc atgcccaatg cagaaggtac ttcaactgta aaagtattca aagcatttac
      721 agaggatatt aacgggcttg tgcaagaact taagcaagca caggtaacgc gtgttgccat
      781 ggaggctacc ggagtttatt ggatggcaat atttgaaatg ctcgagcaag ccggtttaca
      841 agtaacactt gtaaatgccc accactttaa aaacacagac gcacaaaaaa cagatgttaa
      901 ggattgtcaa tggctacatc aattgcatga gcatggactt ttacgagcct cacatgttgc
      961 ctcagaaagc tatcgtcaat tgcgtaccta tctgcacgaa cgcaatgtgc tgcaaaagca
     1021 aaaaagcgat ggcttaaatc ggatacaacg agcacttaca caaatgaatt taaaaataca
     1081 acatttgata agcgatatag aaggggtatc gggaatgaaa attattcgcg ccatagcaaa
     1141 tggaacacag gatccttatc aactactatc agaagtcaac gttaaagcac tcaaagccac
     1201 agagggtgat ttgataaaat cattaaccgg taattacaaa gagcatcacg tgcaagtt
//
LOCUS       DAOMAA010000800         1257 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_139530_length_1257_cov_2.133943, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000800 DAOMAA010000000
VERSION     DAOMAA010000800.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139530_length_1257_cov_2.133943"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1257
                     /locus_tag="PLO59_09810"
     CDS             <1..>1257
                     /locus_tag="PLO59_09810"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HQV01444.1"
                     /translation="YAMPGYMQGTAFAGYNNNDYISAVQLTGENGTQIQNYDHDQPCA
                     ACTVTALNPLLCNRLACHPPDFTAFKPENKSSFQNRTVQLQLGKGVRNGTAPYNLFVQ
                     AGAWPFANNSMAVFIDWNRDGDFDDTYNGPGGVIAETYGTHQLPAGDLNNYPVSSWPL
                     GTQWSIDVPDKTDLVYDNNDVTQLPNNSSSSIFTGNVRMRVREVYGASNVHPFSNNYA
                     FGEIEDYYIQVLDNCPDMSSNVCKWIGTTADWNTATNWCPALPTANDIAYIGPVSTSF
                     FNPVIGDSTQAVCRALHITDGAKLTADAPTGTSSLKIRDDLIIGFNAPSSSNASFNVK
                     SKLEGLITLPGMQSTPPAGIAVPAITPFKQNAHAKLQVAYTKAELIRTFGLKAGDVID
                     TLFTLSKDVLSPLPPFQTTLQNFRIIA"
BASE COUNT          364 a          281 c          271 g          341 t
ORIGIN      
        1 gtatgccatg cccggatata tgcagggtac tgcttttgca ggctataaca ataacgatta
       61 cataagcgct gttcaattaa caggcgaaaa tggcacacaa atacaaaact acgatcacga
      121 ccagccttgt gccgcttgta ccgttacagc tttaaacccg ttactttgca accggctggc
      181 ctgccacccg cccgatttta ctgcatttaa gcccgaaaac aaaagcagtt ttcaaaaccg
      241 tacggttcaa ttacaattag gcaaaggagt tagaaacggc actgcaccct ataatttatt
      301 tgtgcaggcc ggtgcatggc cttttgccaa caacagtatg gctgtattta ttgactggaa
      361 tcgcgatggc gattttgatg atacctataa tggcccgggt ggtgttattg ccgaaacata
      421 cggcacgcat caattgccgg caggcgactt aaataattac ccggtaagta gttggccgct
      481 tggcacgcaa tggagtattg atgtgcccga caaaaccgat ttggtttatg ataataacga
      541 tgtaacacag ttaccaaaca atagcagtag cagtattttt accggtaatg tgcgcatgcg
      601 cgttcgcgaa gtttatggag ccagcaatgt gcatccgttt tctaacaatt atgcttttgg
      661 cgagatagag gattactaca tacaggtttt agacaactgc cctgatatga gcagtaatgt
      721 gtgtaaatgg ataggcacca ctgccgattg gaatacggct accaactggt gcccggcatt
      781 gcccactgcc aacgacattg cgtatattgg ccctgttagc acttcgtttt ttaatccggt
      841 tattggtgat agtacacaag ctgtttgccg cgctttgcat ataacggatg gtgctaaact
      901 tacagccgat gcaccaaccg gaacatccag tttaaaaata cgagacgatt tgataattgg
      961 ttttaatgcg cccagcagca gcaatgcatc atttaatgta aaaagcaaat tagaaggact
     1021 tattacatta cccggtatgc aaagtacacc gcctgcaggt attgctgttc cggctattac
     1081 accctttaag caaaatgcac atgcaaaact tcaggtagct tacaccaaag ccgaactgat
     1141 acgcaccttt gggctaaagg caggtgatgt tattgataca ctgtttacat taagtaaaga
     1201 tgtgctttcg cctttgcctc catttcaaac tacattacaa aatttcagaa ttatagc
//
LOCUS       DAOMAA010000801         1254 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_140179_length_1254_cov_2.093411, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000801 DAOMAA010000000
VERSION     DAOMAA010000801.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140179_length_1254_cov_2.093411"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="PLO59_09815"
     CDS             complement(<1..320)
                     /locus_tag="PLO59_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HQV01445.1"
                     /translation="MGSQKVNQSFTENYDFIRNIAELAALEVNGRSEFKTEVQFDNTF
                     SGSLNKYLFEKTAFVRIPYVAKYGVDMQNARVITQMADTMLTVHLTPAKLLSLELKVN
                     QVQT"
     gene            687..1238
                     /locus_tag="PLO59_09820"
     CDS             687..1238
                     /locus_tag="PLO59_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-dependent hydrolase"
                     /protein_id="HQV01446.1"
                     /translation="MTNSNNNQMELAYPIGKHQMHSHYESKWVLQAIEDVSKLPYDLL
                     IATKDLSQSQLSTSYRPGGWTLRQVIHHLADSHMNAYCRFKLGLTETIPSIKPYNEAL
                     WATLPENETTPVLVSIQLLEALHLRWVNTLSAMHETDFSRNVIHPQYMKSFTLWQLLS
                     LYQWHGKHHVAHITQTIKINGWN"
BASE COUNT          462 a          222 c          191 g          379 t
ORIGIN      
        1 attgtttgta cctgatttac ttttaactct aaactcagta atttggcagg tgtaaggtgt
       61 actgtaagca tcgtatcagc catttgtgtt attacacgtg cattttgcat atcaacacca
      121 tattttgcaa cataaggaat gcgcacaaat gcagtttttt caaacagata tttatttaaa
      181 ctgccgctaa atgtattatc aaactgtact tcagttttaa attcgcttcg gccattaacc
      241 tccaatgcag caagttcagc aatgtttctt ataaaatcat agttctcggt aaatgattga
      301 tttacctttt gagatcccat tctttttcca ataaaaaaag ctgcaataac cagtactaat
      361 agtaagatga aagttatata ccgaagataa gtttgcataa taagtattgt tgagagatta
      421 aattcagacg ttaatagaaa tttataagtg ttttagcgct attaaatata attttgccac
      481 aattataaaa ggatccgatt ttaaaagaaa aatactgctt ttcaatattc aaaatcagca
      541 tattaatggg ctcaaacaat catgtattag attacaaagt tagttcgata gattcagatt
      601 aaattttaat caactaatta taaaagttac ataattaaac ctcatagttt aaaaatccaa
      661 tcgaaaataa tagcacaatc caaaccatga cgaattcaaa caataaccaa atggagctgg
      721 catatcctat aggtaaacat caaatgcata gccattatga aagcaaatgg gttttacaag
      781 caatagaaga tgtaagtaag ttaccatatg atttactaat cgcaaccaaa gatttaagcc
      841 aaagccaatt aagcacaagc tatagaccgg gtggttggac actgcgacaa gtaatacacc
      901 acctggccga ttcgcacatg aatgcatact gccgatttaa actgggttta acagaaacaa
      961 tcccatccat taaaccatac aatgaagcgc tatgggctac tttacccgaa aatgaaacca
     1021 caccggtttt agtgtcaata caattgttag aagccttaca tttgcgttgg gtaaacacat
     1081 tgtcggccat gcatgaaact gattttagcc gaaacgttat tcatccacaa tacatgaaat
     1141 cttttacgct ttggcaatta cttagtttgt atcaatggca tggcaagcac catgtagcac
     1201 acataacaca aacaataaaa attaacggat ggaattaaat tttataaaat ctaa
//
LOCUS       DAOMAA010000802         1252 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_140531_length_1252_cov_3.404344, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000802 DAOMAA010000000
VERSION     DAOMAA010000802.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140531_length_1252_cov_3.404344"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PLO59_09825"
     CDS             complement(<1..182)
                     /locus_tag="PLO59_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidylprolyl isomerase"
                     /protein_id="HQV01447.1"
                     /translation="MKKSLIHLCVVAAVVLAASCKKGIQKTAGGLEYMIHTNNSGPKP
                     KEGDIMRCDIVYKIVK"
     gene            complement(185..730)
                     /locus_tag="PLO59_09830"
     CDS             complement(185..730)
                     /locus_tag="PLO59_09830"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HQV01448.1"
                     /translation="MLRQSLYFLTSLVVSASCIGCKSKQTEEFAQKQAQLSNQLVAAH
                     KSYVKEEAAIIDTFLVQKNLKLQQTPTGLRYKIYSSSNGYTPVNGDEVTATYHLYLLN
                     DKLCQSNNQMRFTVGMADIPTGLHELVQLMHAGDSAHAVLPVHLAYGLSGDANIPPES
                     TLFCTFKINNIKQNQINNSKK"
     gene            complement(730..>1252)
                     /locus_tag="PLO59_09835"
     CDS             complement(730..>1252)
                     /locus_tag="PLO59_09835"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HQV01449.1"
                     /translation="NIYTGILTDTGSFKFSSTTGDVHRIIAHLIDTGVVPNEIHEKIY
                     DNFSESRTRFLGYCLEQKLVVLQPYNIAYIAVTSAELKLFKHETGDTEGIVNYALGIK
                     GMRMAAFFTEQDNGVKISFRSKGTFSVKALAAAHFNGGGHLNAAGGKSPENLEQTITK
                     FIALLPFYENQLV"
BASE COUNT          343 a          246 c          239 g          424 t
ORIGIN      
        1 ttcttcacaa ttttatatac aatatcgcag cgcataatat caccttcttt aggtttgggg
       61 ccgctgttgt ttgtgtggat catatactcc aaaccaccgg ctgttttttg aattcctttt
      121 ttacaagatg cagctaatac cactgcagca accacacaaa ggtggataag cgactttttc
      181 atttttactt ttttgaatta ttaatttgat tttgtttgat gttattaatt ttgaatgtgc
      241 aaaacagggt gctttcgggt ggaatatttg catcgccact taaaccatag gcaaggtgta
      301 cgggcaatac tgcatgggcc gaatcgcctg catgcatcag ttgcaccagt tcatgtaagc
      361 ctgttggtat atcggccatg cctactgtaa accgcatctg attgtttgat tggcacagtt
      421 tgtcatttaa cagatataaa tggtaggtag ctgttacttc atcaccatta acaggtgtat
      481 aaccattact actgctgtat attttatagc gtagtccggt gggtgtttgt tgaagtttta
      541 aatttttttg aaccagaaaa gtatcaatta tagcagcctc ttcttttaca tagcttttat
      601 gtgcagcaac taactgattg ctaagttgtg cttgcttttg tgcaaactcc tcggtttgtt
      661 tcgacttgca acctatgcaa gaagcgctaa caactaagct ggttaaaaag taaaggcttt
      721 gcctaagcat tatactaact ggttttcgta aaagggcagc aaagctataa attttgttat
      781 ggtttgttcc agattttcgg ggctcttgcc gcctgctgca tttaggtggc caccaccgtt
      841 gaaatgcgct gctgctaagg cttttacact aaaagtacct tttgatctga acgaaatttt
      901 aacaccatta tcttgttcgg taaaaaaggc tgccatgcgc atacctttaa tgcctaaggc
      961 ataatttaca atgccttcgg tatcacccgt ttcatgttta aacaacttta attcggctga
     1021 tgtaactgct atgtaagcta tattataagg ttgcaacacc accagtttct gttccaaaca
     1081 gtagcctaaa aaacgcgtac ggctttcaga aaaattatcg taaatttttt catgtatttc
     1141 attgggtacc acaccggtat caatcaaatg tgcgataatg cgatgcacat cgcctgtagt
     1201 gctcgaaaat ttaaaagaac ccgtatcggt taaaattccc gtatatatat tt
//
LOCUS       DAOMAA010000803         1252 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_140559_length_1252_cov_2.766917, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000803 DAOMAA010000000
VERSION     DAOMAA010000803.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140559_length_1252_cov_2.766917"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="PLO59_09840"
     CDS             complement(<1..318)
                     /locus_tag="PLO59_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012924960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HQV01450.1"
                     /translation="MATALSVKHLIGIKDLTADDIELIFSTAENFKEVINRPIKKVPS
                     LRDITVANIFFENSTRTKLSFELAEKRLSADVINFSTSGSSVSKGETLIDTVNNILAM
                     KVDM"
     gene            complement(340..885)
                     /gene="pyrR"
                     /locus_tag="PLO59_09845"
     CDS             complement(340..885)
                     /gene="pyrR"
                     /locus_tag="PLO59_09845"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008242836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HQV01451.1"
                     /translation="MQSRLILNSQAFEITIRRLCYQLIENHHNFEHSAIIGLQPRGIF
                     LARRIVTMLDAWLPNTPVLYGELDITFYRDDFRRRAEVAKANATKIDFIIEDKKVVLV
                     DDVLFTGRTIRAGLDALLAFGRPRKVELLTLIDRKFARQLPISPNYTGHAIDSIDADT
                     VSVQWKETDLNDSVWLHTTNT"
     gene            1005..>1252
                     /locus_tag="PLO59_09850"
     CDS             1005..>1252
                     /locus_tag="PLO59_09850"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQV01452.1"
                     /translation="MKTQNNSSYILPDKTVEVSHGLHINFTETGKGKTLLFIHGLANF
                     AQVWQLQQNKLSSFRCIAIDLPGCGHSSKGYLPYSMVY"
BASE COUNT          372 a          234 c          231 g          415 t
ORIGIN      
        1 catatcaacc ttcatggcca atatgttgtt taccgtatca attaatgttt cacctttact
       61 tacgctactg ccgctggtgc taaaatttat cacatcagca cttaaacgtt tttcggcaag
      121 ttcgaatgat agtttggtgc gtgtactgtt ttcgaaaaaa atattggcta ctgtaatatc
      181 tcttaacgag ggtacctttt taatgggcct atttattacc tctttaaaat tttcggctgt
      241 agaaaaaata agttcaatat catcggcagt caggtcttta atgcctatca gatgtttaac
      301 acttaatgca gtggccatta attctttaat ttttcagtat caggtattag ttgtatgcag
      361 ccaaacgctg tcgtttaaat cggtttcctt ccattgcaca cttacggtat ctgcatcaat
      421 actatcaatt gcatggcctg tgtaattggg cgatataggt agctggcggg caaattttct
      481 gtcaatcaag gtaagcaatt ctactttgcg tggtctgcca aatgccagta aagcatctaa
      541 gccagcgcgt atggtacggc ctgtaaaaag aacatcatca actaaaacca ctttcttatc
      601 ttcaattata aaatcaattt tagttgcatt ggccttcgct acttctgctc tgcgcctaaa
      661 atcatcacga taaaatgtta tatctaactc tccatacaat accggtgtgt taggcaacca
      721 tgcatcaagc atggttacaa tgcgtctggc taaaaaaata ccacgcggtt gcaagccgat
      781 aattgccgaa tgttcaaaat tatggtgatt ctcaatcaac tgatagcaaa gcctgcgtat
      841 ggtaatttca aaagcttgag aatttaatat taatcgcgat tgcatacgct ggtgtgaaga
      901 cttatgttgg gacaaatgta aaatttatgt ggtgttacca cgcaataaaa atgtgatttg
      961 atatcgtatt actttatttg cacactttat agctataaat catattgaaa actcaaaata
     1021 attcaagtta cattttaccg gataaaacgg ttgaggtttc gcacggtttg catattaatt
     1081 ttacggaaac cggtaaaggc aaaaccttat tatttattca cggtttggcc aattttgcgc
     1141 aagtgtggca attgcaacaa aacaagttaa gcagtttccg ttgtattgca attgatttgc
     1201 ccggatgtgg ccattcgagt aaaggatatt tgccttatag catggtttat ta
//
LOCUS       DAOMAA010000804         1252 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_140639_length_1252_cov_2.076023, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000804 DAOMAA010000000
VERSION     DAOMAA010000804.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140639_length_1252_cov_2.076023"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..613)
                     /locus_tag="PLO59_09855"
     CDS             complement(59..613)
                     /locus_tag="PLO59_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQV01453.1"
                     /translation="MVATQNDLLERTVFAERKRLFSFIRNKVATDEEAEDILQDVFYE
                     LTETVRMTKPIERVAAWLFTVARNKITDRYRKKKPEALANLAAQGTNDEGESFFLTDL
                     LSQQSDNADTNLLRKTIMIAIQEALAELPQEQRNVFVWHELDDMSFNDIAEKTGVSVN
                     TLLSRKRYAIMHLRKKLKHLYDEL"
     gene            complement(694..>1252)
                     /locus_tag="PLO59_09860"
     CDS             complement(694..>1252)
                     /locus_tag="PLO59_09860"
                     /inference="COORDINATES: protein motif:HMM:NF015866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HQV01454.1"
                     /translation="DFYYSDEAFSIMERDISACEKAGAAGVVFGLLLPDGTVDVNRVK
                     QLVELAYPMDVTFHRAFDMSRNLFEALDAIVACGIKRILTSGGYAKAIDGINEIQQLI
                     EYSAGRVTIMAGSGLNDKNLVDFFNAGVTEFHASATKFISSPMQYHNNRILFEQYPGM
                     QHQMQTVDGNQIAAMRNTLNKFKKH"
BASE COUNT          376 a          261 c          200 g          415 t
ORIGIN      
        1 tcttttattc ttattcatct gaaactattt aagttgtacg tttgaaaatg cattggcttt
       61 atagctcatc ataaagatgt ttgagttttt tacgcaaatg cataattgca tagcgtttgc
      121 gcgatagcaa tgtatttacc gaaacacctg ttttctctgc aatatcatta aagctcatat
      181 cgtctaactc atgccacaca aatacattgc gttgttcctg tggcaattcg gctaatgcct
      241 cctgaatagc aatcattatg gttttgcgta ataaattagt atcagcatta tcactttgct
      301 ggctcaataa atcagtaaga aaaaaagact caccttcatc gtttgtacct tgtgcagcca
      361 aattagccaa agcttctggt ttttttttcc gatagcgatc ggtaatttta ttacgtgcta
      421 ctgtaaataa ccaagctgca acacgctcaa taggtttggt cattcgtacc gtttcagtaa
      481 gctcataaaa tacatcttgc agtatatctt cagcttcttc gtcagtagca actttattac
      541 gaataaaact aaataaccgt ttgcgctcgg caaaaacggt acgttccaac aaatcgtttt
      601 gtgttgcaac cattaactgt gtatttgaca acaaagtatg catataaaac aaagtcggcc
      661 gtcagaaaaa tttattttat gcatcaacaa cttttagtgc tttttaaatt tgtttaacgt
      721 attgcgcatg gctgctattt gatttccatc tacggtttgc atttggtgtt gcatacccgg
      781 atattgttca aacaaaatcc gattgttatg atactgcatg ggtgatgaaa taaatttagt
      841 ggccgatgca tgaaactcag taacaccggc attgaaaaaa tcaaccagat ttttatcatt
      901 taaaccactg cctgccataa tggtaacacg ccctgcacta tattctatta actgttgtat
      961 ttcatttata ccatcaatag cttttgcgta accacccgag gtaagtatgc gttttatgcc
     1021 acatgcaact attgcatcta acgcttcaaa taaatttcga ctcatatcaa atgccctgtg
     1081 aaatgttaca tccattggat acgcaagttc gactaattgt tttactctgt ttacatccac
     1141 cgttccatcg ggtaacaaca aaccaaaaac cacacctgct gcaccggctt tttcgcaggc
     1201 actaatatct ctttccataa tgctaaaagc ttcatcagag taatagaaat cg
//
LOCUS       DAOMAA010000805         1249 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_141119_length_1249_cov_3.173367, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000805 DAOMAA010000000
VERSION     DAOMAA010000805.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141119_length_1249_cov_3.173367"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="PLO59_09865"
     CDS             complement(<1..669)
                     /locus_tag="PLO59_09865"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQV01455.1"
                     /translation="MLTDNYNRTHNYLRISLTDACNFRCTYCMPNEDMLFTPAQHLMQ
                     THEIIKLAHFFVQQGIDKIRLTGGEPLIRKDFDAIVTALSKLGITLTLTTNARLLHHH
                     LFHLINSGVKSINISLDTLKPDRFLKITRRNDFEKVKANIFLCLEQAIRVKINVVLMH
                     GINDDEILDFIRLTKHHPLHVRFIEFMPFDGNQWQADKVITHYEIIAKAQQTFDIIKM
                     KDAVN"
     gene            complement(704..>1249)
                     /locus_tag="PLO59_09870"
     CDS             complement(704..>1249)
                     /locus_tag="PLO59_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013927754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HQV01456.1"
                     /translation="TNQTGWDMKQVSPPLKSYIDGISDKNTHILIPGCGNAYEAKYLL
                     DRGFTNVTIVDIATPLTKQLQQQFGNNQHIKIINDDVFNLNDTYDLILEQTFFCALPL
                     HLRTNYVSKMYNLLKPSGKLAGLLFASEFAAPGPPFGGKIADYQKLFGVHFTNVKLEP
                     CYNSIEQRTGNELFIIAKKES"
BASE COUNT          381 a          210 c          219 g          439 t
ORIGIN      
        1 attaacggca tccttcattt taattatgtc gaaagtttgt tgggccttag caataatttc
       61 ataatgggtt attactttat cagcttgcca ttgattaccg tcaaatggca taaattcaat
      121 aaaccttaca tgcaaagggt ggtgttttgt aagtcttata aagtctaata tttcatcatc
      181 gttaatgcca tgcattaaaa ccacatttat ctttactcgg atagcttgtt ccaaacataa
      241 aaaaatattg gccttcactt tttcgaaatc atttctgcgg gtaattttta aaaacctatc
      301 aggttttaaa gtatctaagc ttatatttat actctttacg cctgaattaa ttaaatgaaa
      361 taaatggtgg tgcagtaacc gtgcattggt tgttaacgtt agggttatac ctaattttga
      421 taaagcggtt acaatagcat caaaatcttt tcgtattaaa ggttcgccac cggttaatct
      481 gattttgtca atgccttgct gtacaaaaaa atgtgcaagt tttattatct cgtgcgtttg
      541 cattaaatgt tgtgccgggg taaacaacat atcctcatta ggcatacaat aggtacacct
      601 gaaattgcat gcatcggtta aagaaatgcg caaataatta tgagttctgt tataattgtc
      661 ggttaacatc accaatcgtt tcaattattt ataaggtgta aatctaactt tctttttttg
      721 caattataaa cagctcattg ccggttcttt gttcgataga gttgtagcag ggttccaatt
      781 ttacatttgt aaaatgtacg ccaaacaatt tttgatagtc tgcaatttta ccgccaaaag
      841 gtggccccgg agcagcaaac tcgctggcaa acaacaggcc tgctaatttc ccggatggtt
      901 ttagcaggtt atacattttt gaaacatagt ttgttcttaa atgtaatggc agggcgcaaa
      961 aaaaagtttg ttccaaaatc aaatcataag tatcgtttaa gttaaaaaca tcgtcattaa
     1021 taattttaat atgttggtta ttaccaaatt gttgctgcaa ttgcttggtt aatggcgttg
     1081 caatatcaac aatggtaaca ttcgtaaatc ctctatccaa taaatatttg gcttcatagg
     1141 cattaccgca gccgggtatt agtatatgtg tgtttttatc tgaaatacca tcaatgtatg
     1201 attttaaagg tggcgaaacc tgcttcatat cccaaccggt ttggttggt
//
LOCUS       DAOMAA010000806         1248 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_141354_length_1248_cov_2.788768, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000806 DAOMAA010000000
VERSION     DAOMAA010000806.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141354_length_1248_cov_2.788768"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..953
                     /gene="alr"
                     /locus_tag="PLO59_09875"
     CDS             <1..953
                     /gene="alr"
                     /locus_tag="PLO59_09875"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HQV01457.1"
                     /translation="HSDYLAVAYADEGVALRQAGIKLPIMVMNPDEQSFDSIVDFELE
                     PEIYSMRLLAAYNQAAQRKSIKARKKHFSVHIKLDTGMHRLGFEMKEITPLVNHLKNW
                     PHIKVASIFSHLAASDAPQHDAFTQNQIDLFSTISNKLITGLGYQPLLHILNSAGVVR
                     WPQAQFNMVRLGIGLHGIGNTDAELALQPVASLHTTISQIKTIAAGETIGYNRSGIAL
                     NDMRIATVAIGYADGYLRKLGNGVGRMMIHGKLVPVVGNICMDMCMLDVTGIDCKEGD
                     DVLVFGADYPIQQIAAQLQTIVYEVLCSISQRVKRVYYFE"
     gene            complement(991..>1248)
                     /locus_tag="PLO59_09880"
     CDS             complement(991..>1248)
                     /locus_tag="PLO59_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015030152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HQV01458.1"
                     /translation="TIEETNGSWLFTFTYTGKHITVEKGSITVNGISLTVVNSQAQQF
                     SVAIIPYTFANTNLHALKTNGIVNLEFDIVGKYMQRIMELR"
BASE COUNT          368 a          218 c          277 g          385 t
ORIGIN      
        1 atcacagcga ttatttagca gttgcttatg ccgatgaagg tgtggcttta cggcaggcgg
       61 gtattaaatt acccattatg gttatgaatc cggatgaaca gagttttgac agcattgttg
      121 attttgagtt ggagcccgaa atttacagta tgcgtttgct tgcagcttat aatcaagcgg
      181 cacaacgtaa atcaattaaa gcgcgtaaga aacattttag cgttcatata aagttagata
      241 ccggcatgca caggcttggt tttgagatga aagaaataac accattagtt aaccatctga
      301 aaaactggcc acatataaaa gtggcaagta tattttcgca tttagcagca agcgatgcac
      361 cacaacatga tgcctttaca caaaaccaaa ttgatttatt ttcgactata agcaataaac
      421 ttattaccgg tttgggttac caaccactgc tgcatatttt aaattcggca ggtgtggtgc
      481 ggtggccgca ggcgcagttt aacatggtac gtttgggcat agggttgcat ggcattggca
      541 ataccgatgc cgagctggca ttgcaacccg ttgcttcgtt gcatacaacc atatcacaaa
      601 ttaaaaccat tgcagccggt gaaactatag gttataaccg tagtggtatt gcacttaatg
      661 atatgcgcat agcaacggtt gccattggtt atgctgatgg ttatttacga aagttaggca
      721 atggcgtggg ccggatgatg atacatggta agttagtgcc tgtagttgga aacatttgca
      781 tggatatgtg catgttagat gtaaccggta ttgattgcaa agaaggtgat gatgtattgg
      841 tgtttggtgc tgattatccc atacagcaaa tagcagcaca attgcagacc attgtttatg
      901 aagtgctttg cagcatatcg caacgggtta aacgcgtgta ctatttcgaa taatgttttg
      961 tatagattac taaaataatt tcgaaattgt ttagcgcaat tccatgatgc gttgcatgta
     1021 tttacccaca atatcaaact ctaaatttac tatgccgttt gttttaagtg catgcaggtt
     1081 ggtgttagca aaagtgtaag gtattatagc tacactaaat tgttgtgcct ggctattaac
     1141 aacggtaagg ctaataccgt taacggtaat cgaacctttt tcaactgtaa tatgcttacc
     1201 tgtatatgta aatgtaaata accaactccc atttgtttct tcaatggt
//
LOCUS       DAOMAA010000807         1244 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_142161_length_1244_cov_3.798150, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000807 DAOMAA010000000
VERSION     DAOMAA010000807.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142161_length_1244_cov_3.798150"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1244
                     /locus_tag="PLO59_09885"
     CDS             <1..>1244
                     /locus_tag="PLO59_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HQV01459.1"
                     /translation="NQAAIQPVVETLKSMSEPERRFTYAAVKQVLRLTANEPSESRTN
                     LQHWLYQFKSQLNDRYHSHLTSQSQWSALKIKQVDAIYDGSPAVNGYEVLIANNDALL
                     AKYPAMLTFGEDVGKIGGVNQTYAGLQQKYGELRVIDTGIREATIIGQGIGLALRGLR
                     PIAEIQYLDYLVYALTTLTDDLACLHYRTKGGQKAPLIVSTRGHRLEGVWHAGSPMAL
                     LLGSLHGMHICVPRNMTQAAGFYNLLMQADDPALVIECLNGYRLKEEMPTNLSEFTVP
                     LGIPEILNEGNDVTIVTYGSTIRVVQEAMTHLALLNISCELIDVQTLLPFDINHSISQ
                     SIKKTNRVIFVDEDVNGGATAFMMQQVLELQNGYQYLDSAPVTVTSYNHRPAYSSDGD
                     YFSKSNVEDIIDAVYKLMHEAMP"
BASE COUNT          400 a          243 c          259 g          342 t
ORIGIN      
        1 aaccaagctg ccatacaacc ggtggttgaa acgctcaagt caatgtcgga gcccgaacgc
       61 aggtttactt atgcagccgt aaaacaagta ctaagattaa ctgccaatga accttcggaa
      121 tcgcgtacca atttacaaca ctggctttat cagtttaagt cgcaattaaa tgacagatac
      181 cattcgcatt taacaagtca atcacaatgg tcggcactta aaataaagca ggtagatgca
      241 atttatgatg gtagcccggc agttaacggt tacgaagtac taatagccaa caatgatgca
      301 ctacttgcaa aataccctgc catgttaacc tttggcgaag atgttggtaa aattggtggc
      361 gtaaatcaaa cctatgcagg tttgcaacaa aaatatggag agctgcgtgt tattgatacc
      421 ggcattagag aagcaacaat tatcggacaa ggcattggac ttgccttgcg tggcttaagg
      481 cccatagccg aaattcaata cttagattat ttagtttacg cactaactac cttaaccgat
      541 gatttggctt gcttacacta tcgaaccaaa ggtggccaaa aagcgccttt aattgtaagt
      601 acacgcggcc acaggttaga aggtgtttgg catgccggtt cgccaatggc attgctttta
      661 ggtagcttac atggcatgca tatatgtgta ccgcgcaaca tgacacaggc tgctggtttt
      721 tataatttat taatgcaagc tgatgatccg gcattggtta ttgaatgcct aaacggttat
      781 cgcttaaaag aagaaatgcc aaccaactta tccgaattta cagttccttt aggcataccc
      841 gaaattttga acgaagggaa tgatgttacc atagtaactt atggttcaac cataagggta
      901 gtgcaagaag cgatgacgca tttagcactg ttaaacatat cgtgcgagtt aatagatgtg
      961 caaaccttat tacctttcga tatcaatcat agcattagcc aatctattaa aaaaaccaac
     1021 agagtaattt ttgttgatga agatgtaaat ggaggtgcaa cagcttttat gatgcaacaa
     1081 gtgcttgagt tacaaaacgg atatcaatat ttagacagtg cccctgttac ggttacatca
     1141 tacaatcaca ggccagcata tagcagcgat ggcgattact ttagcaaatc taatgttgaa
     1201 gatattattg acgcagttta taaattgatg catgaagcta tgcc
//
LOCUS       DAOMAA010000808         1244 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_142188_length_1244_cov_3.082422, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000808 DAOMAA010000000
VERSION     DAOMAA010000808.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142188_length_1244_cov_3.082422"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="PLO59_09890"
     CDS             complement(<1..828)
                     /locus_tag="PLO59_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015808136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HQV01460.1"
                     /translation="MIKNIKFVEVKSEIGAGTRGASMGIDAIKVAALDYGSNLFSQIP
                     SVEIPNENKMLYEQVGSLYAKRIKGVIKIQERLVTELGAIFKAKQFPIVLAGDHSSAA
                     GSVAGIKAAFPKSKLGIIWIDAHADLHSPYTTPSGNMHGMPLSAILNEDNMACKINKP
                     DKETIDLWNKLKKVGGMGPKITYKDLVYIAVRDIEPEESYLIKKNKIKTFTTNEVKRN
                     GVEKIARDALAHLNHCTHIYVSFDVDSMDSSISKGTGTPVRNGITEKEAGSLCVRLIQ
                     "
     gene            957..>1244
                     /locus_tag="PLO59_09895"
     CDS             957..>1244
                     /locus_tag="PLO59_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HQV01461.1"
                     /translation="MKNKYVDLIKQTFEFPQDGFQVVDDQLHWNDIPLLDIIAQYGTP
                     LKLSYLPKISSQIQRAKRIFNVAMARADYKGNYTYCYCTKSSHFSFVLDEAL"
BASE COUNT          365 a          265 c          199 g          415 t
ORIGIN      
        1 ttgaattaat cgcacacata aactacctgc ttctttttct gtgataccgt ttcgaacggg
       61 ggtgcccgtg cctttactta tactgctgtc catgctatct acatcaaacg aaacataaat
      121 atgtgtacaa tggtttaagt gtgccaaagc atcacgggca attttttcga caccattacg
      181 ctttacttca ttggtagtaa acgttttaat tttatttttt ttaatcaaat agctttcttc
      241 cggctcaata tcgcgtacgg caatataaac caggtcttta taagttattt tagggcccat
      301 gccacccact ttctttagtt tattccaaag gtcaatggtt tccttatcag gcttgttaat
      361 tttacaggcc atattgtctt cattcaaaat tgccgacaaa ggcataccat gcatattgcc
      421 cgaaggcgtg gtgtagggac tatgcaagtc ggcatgggca tcaatccaaa taatgcccag
      481 cttcgattta ggaaaagcag ctttaatgcc tgcaaccgat ccggctgcag agctatggtc
      541 gccagctaat acaatcggaa actgtttggc cttaaatatg gcacctaact ctgttaccaa
      601 acgctcttga attttaatta cccctttaat ccgttttgca taaagtgagc ccacttgctc
      661 atataacatt ttgttttcat tgggtatttc taccgatggt atctgcgaaa acaagttgct
      721 tccatagtct aaagcagcta ctttaatggc atcaatgccc atgcttgcac cacgggtacc
      781 ggcaccaatt tcgcttttaa cctctacaaa ctttatgttt tttatcataa ttaattttgt
      841 ttttttgctt aaaattaaac atccagtcta attacattgc tatttattta tcaatctaag
      901 ttagttacat ttcgttaaac aattcaactt aagcttacat cagtcaaatt cacggtatga
      961 aaaacaaata tgttgaccta ataaaacaaa cattcgaatt tccacaggac ggatttcaag
     1021 tagttgatga ccaactacat tggaacgaca ttcctttatt agacatcatt gctcaatatg
     1081 gcactccatt aaaactcagc tatttaccaa aaataagttc gcaaatacaa cgtgcaaaac
     1141 gtatttttaa tgtagcaatg gcccgtgccg attacaaagg taattatact tactgctatt
     1201 gcactaaaag ttcgcatttt tcttttgttt tggacgaagc actt
//
LOCUS       DAOMAA010000809         1243 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_142444_length_1243_cov_2.674242, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000809 DAOMAA010000000
VERSION     DAOMAA010000809.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142444_length_1243_cov_2.674242"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1045
                     /locus_tag="PLO59_09900"
     CDS             <1..1045
                     /locus_tag="PLO59_09900"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HQV01462.1"
                     /translation="TFHSSSSLDYGDLNMTCIKPFWDKSWTLFADAILTPAFDEKEFN
                     LAKEQMISAAHQTEGDPDGYLEQLAMQNAFGMRNYAKIAAGTATSLEKLTLAETKKYF
                     ETTVGKKRCFLVIVGNVTQADVTAKVMATLGKLQAGTAATSEPKLLITKGSENVIDRD
                     IATNYLMGIMSAPAYNSEDGIPMMVGMNILYDRYFVELRTKRSLSYAPAAGYNREAIT
                     SPINTIYITTQKPKEAMQVMVQVIDSVKQMGFKEKELIDKKQKYLTTTYMKMETSAAQ
                     SFALGRAEACGSYLLDEKFAAKVNAITLAEVNRVFKAYTQAIKWTYLGKKDQVSKEDF
                     KQTIKTDFPAGAH"
BASE COUNT          410 a          242 c          255 g          336 t
ORIGIN      
        1 tacttttcac agcagcagca gtttagatta tggcgattta aacatgacct gtattaaacc
       61 gttttgggat aaatcgtgga ctttatttgc cgatgccata cttacacctg catttgacga
      121 aaaggaattt aatttggcta aggagcaaat gatatcggca gcacaccaaa ccgaaggcga
      181 ccctgatggt tatttagagc aactggctat gcaaaatgct tttggcatgc gtaactatgc
      241 aaaaattgct gcaggcacgg caacaagttt agaaaaacta accctggccg aaaccaaaaa
      301 atatttcgaa accactgttg gtaaaaaacg ttgtttctta gtaattgtag gtaacgttac
      361 ccaagccgat gtaaccgcta aagtaatggc tactttaggc aaactacaag ccggtacggc
      421 agcaaccagc gaacccaaat tgcttattac aaaaggcagc gaaaatgtaa ttgaccgcga
      481 tattgcaacc aattatttaa tgggcataat gagtgcacct gcttataaca gcgaagacgg
      541 catacccatg atggttggta tgaatatttt atacgaccgt tactttgtgg agttgcgcac
      601 caaacgcagt ttatcgtatg cacctgctgc aggctataat cgcgaagcca ttacaagccc
      661 cattaacacc atttacatta ccacacaaaa acccaaagaa gccatgcagg taatggtaca
      721 ggttattgac tcggtaaaac aaatgggttt taaagagaaa gaattgattg ataaaaaaca
      781 aaagtacctt acaaccactt acatgaaaat ggaaacatcg gcagcacaat cgtttgcatt
      841 gggccgtgcc gaagcttgtg gctcttattt gttagacgaa aaatttgcag ccaaagtaaa
      901 tgccataacc ttagccgagg ttaaccgtgt atttaaagct tatacacaag ccattaagtg
      961 gacttactta ggtaaaaaag atcaggtgag taaagaagat tttaaacaaa ccattaaaac
     1021 cgatttcccg gccggagcac attaaatgtt tgatgttatt ggtttgaagc atattattta
     1081 aagtacgtta aacaaaagcg gctgtatcgt ttactgatac agccgctttt gtttgaggca
     1141 actatcttac ttcgtaagct tactgcttta caaacttttt tatggtgtga tgtgttgccg
     1201 tagttaagct gaggtgatac atacccggca taagtgtagc agt
//
LOCUS       DAOMAA010000810         1242 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_142635_length_1242_cov_4.053075, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000810 DAOMAA010000000
VERSION     DAOMAA010000810.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142635_length_1242_cov_4.053075"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1242
                     /locus_tag="PLO59_09905"
     CDS             <1..>1242
                     /locus_tag="PLO59_09905"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HQV01463.1"
                     /translation="EAVAPKPEIAEPKLYRASNTVSHNLKDIKLEVLFDWGKKWLYGK
                     ATLTLAPHFYSSNHVDLNARGMQINEVAIIKNGSKKPLQYVYENDSLKIDLDTIYNRK
                     QSYLIYIDYIAKPDELKSKGGSAAISSDKGLYFINADGKVPNKPRQIWTQGETQSNQV
                     WMPIIDSPNQRMTQEISMTVDTNFKTLSNGLLISSKNNGNGLRTDVWKQSLPAAPYLT
                     MMAVGDFAIIKDKYLNLEVNYFVYPEYANDAKAIFGNTPEMMKFFSEKLVPYPWEKYS
                     QVIAEDYVSGAMENTTATIHGSFVQQHKRELLDGSQEDVIAHELFHQWFGDLVTTESW
                     SNLTINESFATYGEYLWQEYKYGRDAADRGLQADLNTYIAQAKQDDAHLVRFEYEQRE
                     DMFDGISYQKGGRILHMLRKYV"
BASE COUNT          425 a          227 c          256 g          334 t
ORIGIN      
        1 tgaagccgta gctcctaaac ccgaaatagc cgaaccaaag ctatatcgtg caagcaatac
       61 cgtttcgcac aacctgaaag atattaaatt ggaagtcctt tttgattggg gaaaaaagtg
      121 gctttacggc aaagcaacac ttacacttgc cccgcatttt tattcgagca atcatgttga
      181 tttaaatgca agaggtatgc aaattaatga ggtggctatt attaaaaacg gcagcaagaa
      241 accgcttcaa tatgtgtatg agaacgactc tcttaaaatt gatttagata cgatttacaa
      301 tcgcaaacaa tcctatttga tatatataga ttatatagcc aaacccgatg aacttaaatc
      361 taaaggcggc agtgctgcca ttagcagcga taaaggatta tattttatta atgccgatgg
      421 gaaagtacct aataaaccgc gtcaaatatg gacacaaggc gaaacgcaaa gtaatcaggt
      481 ttggatgccc atcattgatt cgcccaacca acgtatgaca caagaaataa gcatgacggt
      541 tgatactaat tttaaaacat tgagcaatgg tttgcttatc agttcaaaaa ataatggcaa
      601 tggattacgt accgatgtat ggaaacaaag tttaccggca gcaccatact taactatgat
      661 ggcagtaggt gattttgcca ttataaaaga caagtaccta aacctggaag ttaattattt
      721 tgtgtatccc gaatatgcaa atgatgccaa agcaattttt ggcaacacac ctgaaatgat
      781 gaaatttttt agcgaaaaat tagttccata cccatgggaa aaatacagtc aggttattgc
      841 cgaagattat gtatcgggcg ctatggaaaa tactacagcc actatacatg ggtcgtttgt
      901 gcaacaacac aagcgcgaat tgttagatgg cagccaggaa gatgtaattg ctcatgaatt
      961 atttcatcaa tggtttggcg atttggttac taccgaatcg tggagtaacc taaccatcaa
     1021 tgaatcgttt gcaacctatg gcgaatactt atggcaagag tataaatatg gacgcgatgc
     1081 agccgaccgt ggcctgcaag ccgacttaaa tacttatata gcacaagcaa agcaggacga
     1141 tgcacatttg gttcgctttg aatatgaaca acgcgaagat atgtttgacg gaatcagtta
     1201 tcagaaaggt ggtcgcatat tgcatatgtt acgtaagtat gt
//
LOCUS       DAOMAA010000811         1242 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_142667_length_1242_cov_3.015164, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000811 DAOMAA010000000
VERSION     DAOMAA010000811.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142667_length_1242_cov_3.015164"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            175..>1242
                     /locus_tag="PLO59_09910"
     CDS             175..>1242
                     /locus_tag="PLO59_09910"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HQV01464.1"
                     /translation="MKTHLLSALVLFTQLLHAQFNSQNITMLSNYDDSTIAAEPALGI
                     RYNGIWGWSDGNGHEYACIGASNGVHIIEVTNPTTPQLRAFIPGCVTNAIWRELKTYS
                     HYLYMVSDDGGPNCFQIADLSYLPDSVHIVHQADTIIRTSHTIFVDKNRLYCGGVRGG
                     VFSTIPAAMAVFDISNPATPVLLRKLSDDYPGISYVHDMYVRNDTVYASAAYQGMHIY
                     RFDTLTNTFNQIASLTQYPASGYNHSSAITGDGNTLIFADEVPTGLPLKSVDISDFQN
                     VTWKTNFTSTLTSTATPHNPFMKYNHYCIVGYYQDGVQIFDVSNASMPIRTGFFDTNP
                     TDGAGLPNPNYSGCWGVYVDLP"
BASE COUNT          374 a          258 c          242 g          368 t
ORIGIN      
        1 agcgactcaa cacaagaatc gtttaccgaa taacaaaata ttataccaca cgtaaagcca
       61 ttgtaaaaaa acgcaatggc tttttttatt ttggatacgt aataaaacac aaaaatatat
      121 acgagtatat tgctgccata tacatttttt tgaaacccct tgtactaaac aaatatgaaa
      181 acacatttac tatcagcact cgtattattt acacagttat tgcatgcgca gtttaactcg
      241 caaaatataa ccatgctatc aaattatgac gattcaacca ttgcagccga accggcatta
      301 ggtatcaggt acaatggtat atggggctgg tcggatggca atgggcatga gtatgcttgc
      361 ataggcgcta gtaatggcgt gcatattatc gaagttacca atccaactac accacaattg
      421 cgtgctttta ttccgggctg tgttaccaac gctatttggc gtgagcttaa aacatatagc
      481 cattatctat atatggtgag tgacgatggc ggccccaatt gtttccaaat agccgatttg
      541 agttacctgc ccgatagtgt acatattgta catcaggcag atacaataat cagaacaagt
      601 cataccattt ttgtagataa aaaccggttg tattgtggtg gtgtacgtgg tggggttttc
      661 agtaccatac cagcagctat ggcagtattt gatatctcaa atccggctac ccctgtttta
      721 ttgcgcaagc ttagtgatga ttacccgggc ataagctacg tgcatgatat gtatgtacgt
      781 aatgatacgg tatatgcatc agcggcatat cagggtatgc atatttatcg gttcgatacc
      841 ttaaccaata cctttaacca aatagcttcg ttaacccagt atccggcatc aggttataac
      901 cacagtagtg ccataaccgg tgatggtaat acattaattt ttgctgatga agtaccaaca
      961 ggcttaccgt taaagtcggt tgatatcagc gattttcaga acgtaacctg gaaaacaaat
     1021 tttaccagca cacttacttc aacagctaca ccacacaatc cgtttatgaa atataatcat
     1081 tactgtattg ttggttatta tcaggatggt gtacagatat ttgatgtaag caatgcctcc
     1141 atgcccatac gtaccgggtt tttcgacacc aatccaaccg atggtgccgg tttgcccaac
     1201 cccaattact cgggctgctg gggtgtttat gttgatttac cc
//
LOCUS       DAOMAA010000812         1237 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_143753_length_1237_cov_3.999154, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000812 DAOMAA010000000
VERSION     DAOMAA010000812.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143753_length_1237_cov_3.999154"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            282..>1237
                     /locus_tag="PLO59_09915"
     CDS             282..>1237
                     /locus_tag="PLO59_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01465.1"
                     /translation="MLLLLIGANTALAQINYVRTTFTSAYTPITVGGGATQINVGTGL
                     GDFSTTGGVASGTEGSAHILWPFNFTYNGVSFTTASDFLGISTNGYVYPVTAAVGGTN
                     AGKLISNVNTNLFTTTSPHATIAPYFDDLSIGTAINGNTGTVLYQVTGSAGSQVLTIQ
                     YTNVPAFSSGSVKALNFQVKFYETTNEIEFHYGPVINGTVAGAYNTSETASIGIESAT
                     GGNTNYIDAITGSKTANTSLITSNKFSQIRNTRFTPGTPVALAAGTYTVGVGQMYTSL
                     SEAFADLNHRGIAGAVTLDLMDAVYDTTVAGGLNIFPLVTSN"
BASE COUNT          379 a          244 c          235 g          379 t
ORIGIN      
        1 caagcatctg ctaatagtag tgcgttaact ttaaataatc aggttcgttt tttaaacgga
       61 actttagagt tagatgcaga gcaaggcaat acattgaagc taacctttat tgacactaac
      121 acaaaccctc cggctactgt tagctattcg tttactaaat aaacccctaa taataaactt
      181 taaactgaat tgttgtaaac ccttatccta tgacaaacat atttacccaa acaaaaacaa
      241 gttggctgaa tacatcacaa aggtttttaa aatcttttgt gatgctattg ctattaattg
      301 gagccaatac agcattagct caaattaact atgtacgaac cacatttacc tcggcctata
      361 cgcctataac tgtaggtgga ggggcaaccc aaattaatgt aggcaccggc ttaggtgatt
      421 tctcaactac gggtggcgta gccagtggca ccgaaggttc ggcccatata ctttggcctt
      481 ttaacttcac ttataatggt gtaagtttta ccacagccag cgacttttta ggtattagta
      541 ctaatggtta tgtatatcct gtaacagctg ctgttggtgg cacaaatgcc gggaaattaa
      601 tatccaacgt aaatacaaac ttatttacta ctacatcacc acatgcaacc attgcacctt
      661 actttgatga tttgtcaatt gggacggcaa taaatggaaa tacaggtacg gtgctttatc
      721 aggttaccgg tagtgccggt agtcaggtgt taaccattca atatactaat gtaccggctt
      781 tttcatcggg aagcgtaaaa gcattaaatt tccaggtgaa gttttatgaa actaccaatg
      841 aaattgaatt tcattatggc ccggttatta atggaactgt tgcgggtgcc tataatacaa
      901 gtgaaacagc ttcgattggt attgaatcgg ccactggtgg taataccaat tatattgatg
      961 caatcacagg ttcgaaaact gccaatacaa gtttaattac ctcaaataaa ttttcgcaaa
     1021 ttcgtaatac acgctttaca cccggtacac ctgtggcttt ggcagcaggt acttacacag
     1081 taggtgttgg tcagatgtac acctcattat ctgaagcttt tgccgattta aaccacaggg
     1141 gcatagcagg tgctgtaacc cttgatctaa tggatgcagt ttatgataca actgtagctg
     1201 gtggtttaaa tattttccca ttagttactt ctaatat
//
LOCUS       DAOMAA010000813         1237 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_143801_length_1237_cov_2.818951, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000813 DAOMAA010000000
VERSION     DAOMAA010000813.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143801_length_1237_cov_2.818951"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="PLO59_09920"
     CDS             <1..496
                     /locus_tag="PLO59_09920"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV01466.1"
                     /translation="TLTVNNFNVHSGTYASIVELKAGNEVTIKATAASTLFNGSEVLI
                     YNTTTLPHCIDNVGYKTGGDNDNENNRKSSKTKLIELKFAANNLKVTAQPNPSKDEFN
                     ITITDAIASAYQLKVFDSFSRLLFQTMISPVYILNLANYPAGIYFLQVQSASSLKTIK
                     LIKQ"
     gene            493..>1237
                     /locus_tag="PLO59_09925"
     CDS             493..>1237
                     /locus_tag="PLO59_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01467.1"
                     /translation="MKTITALTIIMLLITRAAYSQMLWSYHYGSDSTDAVGALVTDND
                     NNVYSTSYLTGNQITFGQMTIPSVFGTNYATISKFNAVGNLVWVKKYGDAGFSQNALH
                     YDKVTNTIVIGGGFAPSIILGPDTLYSSNGGCFLSRTDTAGIPMWGKQFNAGMYLHTT
                     DSLSNIYVVGSTKIAVSLGSFVIQPGVYMSKLDPNGNVLWAKKMFNVYGTGNGLWKVT
                     PKGMKIYNNEIYLTADTKGDTIRVDTTNYP"
BASE COUNT          414 a          216 c          222 g          385 t
ORIGIN      
        1 tacattaact gtaaataact ttaatgtgca tagcggaaca tacgcaagca tagttgaact
       61 aaaagcaggt aatgaagtaa ccattaaagc aacagcagca tccactttat ttaatggcag
      121 cgaagtgcta atttataaca caactacatt gccccattgt attgataatg taggttacaa
      181 aacaggtggc gataatgaca atgagaacaa tagaaaaagc tctaagacta aattaataga
      241 attaaaattt gccgctaata atctcaaagt aacggcacaa cccaatccaa gcaaggatga
      301 atttaatatt acaattacgg atgcaattgc atcagcatat cagctaaaag tatttgatag
      361 ttttagcagg ttgttgtttc aaacgatgat atcgcccgtt tatattttaa accttgccaa
      421 ctatcctgca ggtatttatt ttttacaagt gcaatctgct tcatcactaa aaactataaa
      481 acttattaag caatgaaaac aataacagca ctaactatca ttatgttact tataacacgt
      541 gcagcttact cgcaaatgtt atggagttat cattacggca gcgattctac agatgcggta
      601 ggagcattag ttactgataa tgacaacaat gtctattcta caagttacct aacgggaaat
      661 caaataacgt ttggtcagat gacaatccca tctgtttttg gtacaaacta tgcaacaatt
      721 agtaaattta atgctgttgg caatctggtt tgggttaaaa aatatggtga tgccggtttt
      781 tcacaaaatg ctttacatta cgataaggta actaatacca ttgttattgg tggaggcttt
      841 gcgccatcca ttattttagg ccccgacacg ctttacagtt caaatggtgg atgtttttta
      901 tcccgaactg atactgccgg cattccaatg tggggtaaac aatttaatgc cggtatgtat
      961 ttacatacta ctgattcgtt aagtaatatt tacgttgttg gttccaccaa aattgctgtt
     1021 tcgcttggtt catttgttat tcaacccggt gtttatatgt ctaaattaga ccctaacggc
     1081 aatgtacttt gggcaaaaaa gatgtttaat gtttacggta caggtaatgg tttatggaag
     1141 gttactccta aaggaatgaa gatatataat aatgaaattt accttactgc cgataccaag
     1201 ggtgatacca tcagggtgga tactactaac tatccac
//
LOCUS       DAOMAA010000814         1237 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_143803_length_1237_cov_2.795262, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000814 DAOMAA010000000
VERSION     DAOMAA010000814.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143803_length_1237_cov_2.795262"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..434
                     /locus_tag="PLO59_09930"
     CDS             57..434
                     /locus_tag="PLO59_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010420925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HQV01468.1"
                     /translation="MENLNIEPGIKTPFILFDASAGALTIKGKSIPENAIEFYQDVYA
                     WLDTYVQTPVANTSITIALEYFNTSSSKCLLDIFRKLETLYKSGKSEVLVNWMYEEDD
                     EDMMEAGDDYQTIVKIPFNITKL"
     gene            complement(424..>1237)
                     /locus_tag="PLO59_09935"
     CDS             complement(424..>1237)
                     /locus_tag="PLO59_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01469.1"
                     /translation="IILSGVMVKFDNLNPSISTQTKVPFVGEIMVSRWAFEALAVNQF
                     KNNAYEKHFYPYNKAMSNATYKNNVWQIKMQDMIDSLHQNKYGNQADQKWITVNNELE
                     KEGIHKFSVYSTKQIIAKPVYNDVLNSLQQIKQTQQLIYKNASQLKDSVIATFDAAQL
                     IALEQKYTNTNLSNLVLDKDNFEPIIISHNHLVQRYHPVYTNGSATSCVRSLFYVANK
                     NFFGTYLSTYFVNLCVIWFFTIVLSVTLYFNTLRKILSWFNKRSLKKLPFKA"
BASE COUNT          395 a          192 c          220 g          430 t
ORIGIN      
        1 aaattaatga agcgtatgca ttttttagtc ttacggtttt tgtaaaataa atttaaatgg
       61 aaaatctcaa tatagaaccc ggtataaaaa caccttttat actatttgat gcaagtgcag
      121 gtgcgctaac tataaaaggc aaatcaatac ctgaaaatgc aatcgaattt tatcaggatg
      181 tttatgcatg gcttgatact tatgtgcaaa caccggttgc aaacacatcc ataaccattg
      241 cattagagta ttttaatacc agctcgtcaa aatgtttgct tgatattttc aggaagctcg
      301 aaaccttata taagtcgggc aaaagcgaag tgttggttaa ctggatgtat gaagaagatg
      361 atgaggacat gatggaagcc ggtgacgatt atcaaaccat cgttaaaata ccttttaata
      421 ttactaagct ttaaatggca gcttttttag cgagcgttta ttaaaccaac ttaatatttt
      481 acgtagtgta ttaaagtata atgtaacact tagtacaatg gtaaaaaacc aaattacaca
      541 aaggtttaca aaataagtgc ttaggtaagt gccaaaaaaa tttttattgg caacataaaa
      601 caacgaccgc acacatgaag tagctgagcc attggtataa acagggtggt aacgttgtac
      661 caaatgatta tggctgataa ttatgggttc aaaattatcc ttatcaagaa ctaagttgct
      721 taaattggta ttggtatatt tttgttcgag ggctataagt tgtgctgcat caaaagttgc
      781 tataaccgaa tcttttaact gcgatgcatt tttataaata agttgctgtg tttgcttaat
      841 ctgttgtaaa ctgtttaaca catcgttata caccggtttg gctattattt gttttgtact
      901 ataaaccgaa aatttgtgga taccttcttt ttctaattcg ttgtttacgg ttatccattt
      961 ttgatcggct tgattgccat acttgttttg atgtagcgaa tcaatcatat cctgcatttt
     1021 tatttgccat acattgtttt tatacgttgc attactcatt gctttattgt atggatagaa
     1081 atgtttttcg taagcattgt ttttaaactg atttactgcc aatgcttcga atgcccaacg
     1141 cgataccata atttcgccaa caaagggcac cttggtttgt gttgagatgg atgggtttaa
     1201 attatcgaac tttaccatta caccacttaa aataatt
//
LOCUS       DAOMAA010000815         1236 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_144004_length_1236_cov_3.366638, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000815 DAOMAA010000000
VERSION     DAOMAA010000815.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144004_length_1236_cov_3.366638"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            228..>1236
                     /locus_tag="PLO59_09940"
     CDS             228..>1236
                     /locus_tag="PLO59_09940"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HQV01470.1"
                     /translation="MLHAVVWFLWASNVEGQQAIIYGTISDSKNTPVEFANIAVAGFP
                     VATKTDSVGKYTLQIPADTVLKIVVTSIGFEKQIFTVKLTSGQSILRNFKLESTAIVL
                     PAAQVTTDVRNDPTLQALSPKLLKSLANPSSNFESLLKSTMAVVSNNELSSDYSVRGG
                     SFDENLVYVNDVQIYRPFLIRSGQQEGLSFVNGSMVESVSFSAGGFEACYGDKLASVL
                     DVKYRKPRRQFGGSASASLLGGDVMLEGTNKQKSFSYILGLRHKSSKYLLRSIETKGD
                     YLPSATDLQAYLTYQINPKTEIAFLGSYNENKYLVVPSNRETEFGTVNDALRFTVYFE
                     GQ"
BASE COUNT          385 a          217 c          260 g          374 t
ORIGIN      
        1 ttaacttacc tgctgatggt agcttgtcgt ttaaatacac tttaaacgaa cgcaacagcc
       61 aactttatta tgccgggcaa ttgccacaaa ataccttgct caatccaatt gcatgggcta
      121 aatttattga tgcatggaaa aagggcgctt ttaaacgaaa agacgaataa ggaattttgt
      181 tagcgatgat agtaccacac ttagtcaggc ttcttaaatt caaatttttg ctccatgctg
      241 ttgtttggtt tttatgggca tcaaacgttg aagggcaaca agcaataatt tatgggacta
      301 tttccgatag taagaatact ccggtcgaat ttgccaatat tgctgttgca ggcttcccgg
      361 tggctactaa aactgattcg gtcggaaaat atacgcttca aattccagca gataccgtat
      421 taaaaattgt agttacttca atcggtttcg agaaacaaat ttttactgtt aaattaactt
      481 caggtcaaag catacttcga aattttaaat tagaaagtac agctatcgta ttaccggcag
      541 cacaggtaac aaccgatgtt agaaacgatc caacgctgca agctttatca cccaaactat
      601 taaagtcgtt agccaaccca agtagcaatt tcgaaagctt gttaaaatca accatggctg
      661 tggtttcgaa taatgagtta agcagcgatt actcagtacg tggtggcagt tttgacgaaa
      721 atctagttta tgtaaatgat gtgcagatat acagaccatt tttaataaga agcggtcagc
      781 aagagggatt gagttttgta aatggcagta tggtcgaatc ggttagcttt agtgccggtg
      841 gttttgaagc atgttatggc gataaattgg catcggtttt agatgtaaag tatcgtaagc
      901 cacgcaggca atttggtggt agtgcaagtg cgagcttgct tggcggtgat gtaatgctgg
      961 aaggtacgaa taagcaaaag tctttttcct acatacttgg ccttcggcat aaatcatcta
     1021 aatatttatt gcgcagtatt gaaaccaaag gcgattattt accttcggct actgatctgc
     1081 aagcatatct tacctatcag ataaatccaa aaaccgaaat tgctttttta ggcagttaca
     1141 atgaaaacaa atatttagtt gtaccaagta atcgcgaaac cgaatttgga accgttaatg
     1201 atgctttgcg ttttacggta tattttgaag ggcagg
//
LOCUS       DAOMAA010000816         1236 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_144030_length_1236_cov_2.982218, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000816 DAOMAA010000000
VERSION     DAOMAA010000816.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144030_length_1236_cov_2.982218"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /locus_tag="PLO59_09945"
     CDS             <1..836
                     /locus_tag="PLO59_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013550039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQV01471.1"
                     /translation="EGVPGLAKTLAIKSLATAIDTKFSRLQFTPDLLPADLVGTMIYN
                     QKKEEFIVRKGPLFANFVLADEINRAPAKVQSALLEAMQEKQVTIGENTFILEEPFLV
                     LATQNPLEQEGTYPLPEAQLDRFMLKVVIGYPTKEEERLIMRQNLSNEIETVNKVVAV
                     DQILNARQAVRLVYMDEKIEKYILDIVFASRFPKEVKLDKLAALINYGGSPRASINLA
                     LASKAYAFIKRRGYVIPEDVRAVAHDVMRHRIGLTYEAEAENITTEQVINEILNAVEV
                     P"
     gene            891..>1236
                     /locus_tag="PLO59_09950"
     CDS             891..>1236
                     /locus_tag="PLO59_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013547839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HQV01472.1"
                     /translation="METTELLKKVRKIEIKTRGLSNQIFSGEYHAAFKGKGMAFSEVR
                     EYIPGDDIRAIDWNVTARLNHPFVKVFEEERELCVMFLIDMSASGNFGTQKQFKRDLI
                     TELCAVLAFSAIT"
BASE COUNT          418 a          216 c          255 g          347 t
ORIGIN      
        1 tagaaggtgt gcccggcctg gcaaaaacgc tggctataaa atcgttagcc acagcaatag
       61 ataccaaatt tagccgctta caatttacgc ccgatttatt gccggccgat ttagtaggca
      121 ctatgattta caatcaaaag aaagaagaat ttatagttcg taaaggaccg ctgtttgcaa
      181 attttgtatt ggctgatgag attaatcgtg cacctgctaa agtacaaagt gcattgctcg
      241 aagccatgca ggaaaaacaa gttaccatag gtgaaaatac ttttatactg gaagagccat
      301 ttttagtttt agctacacaa aaccctttag aacaggaggg aacctatccg ttacccgaag
      361 cgcaattaga tcgttttatg ttaaaggtag ttatcggcta tccaacaaaa gaagaagagc
      421 gcttaatcat gcgtcaaaac ctgagcaacg aaatcgaaac cgtaaacaaa gtagtggctg
      481 ttgatcaaat attaaatgca aggcaggcag ttcgtttggt ttatatggat gaaaaaattg
      541 agaagtatat tttagatatc gtattcgcat cgcgtttccc taaagaagta aaacttgata
      601 aactggcagc acttataaat tatggtggct cgcctcgtgc aagtattaat ttggctttgg
      661 catcaaaagc ttatgcattt ataaaacgca gaggttatgt tatacctgaa gatgtgcgtg
      721 ctgttgcaca tgatgttatg cgacaccgca ttggattaac ctatgaagcc gaagccgaaa
      781 acattactac cgagcaagta attaatgaaa tattaaatgc cgttgaagtt ccatagattc
      841 aacatatcta ttaaaaaaca ttaccgcatt taatcattta aaattaaggt atggaaacta
      901 ccgagctttt aaaaaaagta cgcaaaattg aaattaagac ccgtggtttg agtaatcaaa
      961 ttttttcggg cgagtatcat gctgccttta aaggaaaagg tatggcattt agcgaagtgc
     1021 gcgaatacat acccggagac gacataagag ctattgactg gaatgttact gccaggctca
     1081 accatccttt tgtaaaagta tttgaagaag aacgcgagct gtgtgtaatg tttttaattg
     1141 atatgagtgc cagtggaaat tttggcacac aaaaacaatt taaacgcgat ttaattacag
     1201 agctatgtgc cgtacttgca tttagcgcca ttacta
//
LOCUS       DAOMAA010000817         1232 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_144910_length_1232_cov_3.446899, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000817 DAOMAA010000000
VERSION     DAOMAA010000817.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144910_length_1232_cov_3.446899"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="PLO59_09955"
     CDS             <1..285
                     /locus_tag="PLO59_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HQV01473.1"
                     /translation="WLGDSPMQAGGKYTIRHTSREAKCLIKDIRYKVDVNTYHRNEED
                     KNIKMNDICRVNVRTTVPLFYDSYRKNRITGSVILIDEATNSTVGAGMII"
     gene            557..>1232
                     /locus_tag="PLO59_09960"
     CDS             557..>1232
                     /locus_tag="PLO59_09960"
                     /inference="COORDINATES: protein motif:HMM:NF015926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HQV01474.1"
                     /translation="MENKETANEQTPDSVQPLHESNTNALAEILEQTAQAQAETSMVT
                     ISEETDAEITLDQSQHQIASEKIVEATGDALHPQGHATTDDEHEILLAAVSHHVAETA
                     EHLEHYEQMNKVELIHALKPVVETDDALSQKQKANAIRDAYQKLTAQERAEALAKFIE
                     DGGVKNDFESRKSADDTTFESLYEKYKNKITAQTAQAEKQRQENLEIKQALLARLKNL
                     IQHEENM"
BASE COUNT          478 a          235 c          223 g          296 t
ORIGIN      
        1 tggttaggcg acagccctat gcaagctggt ggtaaataca ccatcagaca tacatcgcgc
       61 gaagcaaaat gtttaataaa agatatcaga tacaaagtag atgtaaatac ctaccaccgt
      121 aacgaagaag acaaaaacat taagatgaat gacatttgcc gcgttaatgt acgtactacc
      181 gtgccgttat tttatgacag ctatcgtaaa aaccgtatta caggtagtgt tattttaatt
      241 gatgaagcta ccaactcaac cgttggagca ggtatgatta tctaataaca tttaaattta
      301 ttgcccataa aaaaccctgc atgttgcagg gttttttttt gatttaattg aaactttttc
      361 aagtacattt tatcagatga taaaagaaaa tataccccta ctaagaagat attacacttt
      421 actgttttta cagataaaca ctttataaaa aaccacttac tttgcttgct gcaactgcat
      481 aaaataaaag cggaacactt ttgtatagca cttaaaaaca aagcgtatat attacgcctc
      541 ttaattaaac tgaatgatgg aaaataaaga aactgccaac gaacaaacac cggatagtgt
      601 gcaaccctta catgaaagca atacaaatgc gcttgccgaa attttagaac aaacagcaca
      661 agcacaggcc gaaacaagta tggttaccat tagcgaagaa acagatgcag aaataacctt
      721 ggatcaatca caacatcaaa tagcatctga aaaaatagta gaagcaacag gcgatgcact
      781 acacccgcaa ggccatgcaa ctaccgatga cgaacatgaa atattgttag cagcagttag
      841 ccatcatgtg gccgaaaccg cagaacattt ggagcattat gagcaaatga acaaagtgga
      901 attgatacat gcacttaagc ctgttgttga aacggatgac gccttatcgc aaaagcaaaa
      961 agccaatgcc atacgcgatg cctaccaaaa actaaccgca caggaacgtg ccgaagcatt
     1021 ggctaaattt attgaagatg gtggcgttaa aaatgatttc gagagccgta aaagtgctga
     1081 cgacactacg tttgaaagcc tatatgaaaa atataaaaac aaaataactg cacaaacagc
     1141 acaggctgaa aaacagcggc aggaaaattt agaaatcaaa caagccttat tagcacggtt
     1201 aaaaaattta attcagcatg aggaaaatat gc
//
LOCUS       DAOMAA010000818         1231 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_145179_length_1231_cov_2.672619, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000818 DAOMAA010000000
VERSION     DAOMAA010000818.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145179_length_1231_cov_2.672619"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="PLO59_09965"
     CDS             complement(<1..828)
                     /locus_tag="PLO59_09965"
                     /inference="COORDINATES: protein motif:HMM:NF022732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HQV01475.1"
                     /translation="MAISSILFFNAACIAQHAAPFSKTNTPVAPDYSLNSSWLALPFI
                     NDVADSVPLFDTLKTDDANADVFFIYPTSFLKATNDTCWNASVFDTIVNNLSLTRSIT
                     YQATIFNAAGKIYVPIYRQAHYRSFFTSDKLSAAQALEVAYTDVRNAFIYYLKNYNNG
                     RPIIIAAHSQGAYHGRKLLSDFFDSRDSELKLVAAYLIGYPTNVHAFEMIKPCTTATQ
                     TNCFISWRCYNNKTDLNQLPNTDALCVNPLTWDTIHTDAPAKLNAGAVLKDFNKLIKN
                     "
     gene            812..>1231
                     /locus_tag="PLO59_09970"
     CDS             812..>1231
                     /locus_tag="PLO59_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HQV01476.1"
                     /translation="MDDIAKKNFLCIIKVLGTGKFALMWKHIYPHHKKNKFAQCINPI
                     CTMILLDGKIASAHFKVKIQEEVQQIIAAGGKPPHLAAILVGSDGASETYVANKIKTC
                     HEVGFQSTLVRLDPTVSQQTLLDKIQELNTDDNIDGYI"
BASE COUNT          407 a          223 c          241 g          360 t
ORIGIN      
        1 atttttaatc aatttattaa agtctttaag cacggcccct gcatttaatt tagccggtgc
       61 atcggtatgt attgtatccc atgttaacgg attaacgcat aaagcatcgg tatttggcag
      121 ctgatttaaa tcggttttat tattgtaaca acgccaactt ataaagcaat tggtttgcgt
      181 agctgtagtg cagggtttta tcatttcaaa cgcatgcaca ttggttggat aaccaattaa
      241 ataagctgct accaatttta gttcgctatc gcgactgtcg aaaaaatcgg aaagcaattt
      301 gcggccgtga taagcaccct gactatgtgc tgcaataata atagggcgtc cgttattata
      361 atttttaaga taatagataa aggcatttcg cacatcggta taagctacct ctaaagcctg
      421 agcagccgat aatttatcgc tcgtaaaaaa actacggtaa tgtgcttgcc ggtatatggg
      481 cacataaatt tttccggctg cattaaaaat tgttgcctga tacgtaatac tgcgtgtaag
      541 gcttaggtta tttacaatgg tatcgaaaac cgaagcattc caacaggtat catttgtagc
      601 ttttaaaaaa ctggttgggt aaataaaaaa tacatcggca ttagcatcat ctgtttttaa
      661 agtatcgaat agtggtacac tatcggccac atcatttata aaaggtaaag ctaaccaact
      721 gctgttaagg ctgtaatcag gcgccaccgg tgtgtttgtt ttactaaaag gtgccgcatg
      781 ctgggctata caagcagcgt taaaaaataa tatggatgat atcgccaaaa aaaatttttt
      841 atgcataata aaagtattag gtacgggtaa gtttgcactt atgtggaaac acatttatcc
      901 gcaccataaa aaaaataaat ttgcacaatg tattaacccc atttgtacaa tgattttatt
      961 agatggaaaa attgcttcgg cacattttaa agtaaaaatt caggaagaag tgcaacaaat
     1021 tattgccgca ggcggaaaac cgccacacct ggcggccatt ttagttggta gcgatggtgc
     1081 cagcgaaacg tatgttgcca ataaaatcaa aacctgccac gaagtaggct ttcagtcaac
     1141 cttagtacgg ctcgacccaa cagtatcaca acaaacattg ttagataaga tacaagagtt
     1201 aaataccgat gataacatag atggctacat t
//
LOCUS       DAOMAA010000819         1228 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_145850_length_1228_cov_2.912191, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000819 DAOMAA010000000
VERSION     DAOMAA010000819.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145850_length_1228_cov_2.912191"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1228
                     /locus_tag="PLO59_09975"
     CDS             <1..>1228
                     /locus_tag="PLO59_09975"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQV01477.1"
                     /translation="LFLSTYFGRDRFQFRSAESGFKANIPWGNATATLRWNHIFNNKL
                     FANTSLIYTDYDFSFGAGQEDFRFELYSGVRDWNAKVDFNYYPSVKHTVKFGFNYVNH
                     LFTPSTASASQNETVFDLGKPYKIYANDFALYALDDWAINDRLTINGGLRATRFVQYG
                     PFDRYITNSNYEVVDTITYGKGDKVAQYNNLEPRFSLSFLINKNTSVKAAYTQNYQYV
                     HLASISSLSLPTDVWVPSSDRVKPQFATQYAVGFFKNFKKDLYETSVEVYYKDMRNQI
                     EYRDGATPDMDIKNNQDNNFVFGKGWSYGAEFFVKKKYGKFNGWIGYTLAWTLRNFPD
                     LNQGKDFYARYDRRNDLKVVAIYELNKKWSFGATFVYATGNALTLPQQRYLFGGAIDP
                     NNLNTSQFNNPQIYYTY"
BASE COUNT          385 a          217 c          238 g          388 t
ORIGIN      
        1 gttgttttta agcacttact tcggcaggga tagatttcag tttcgtagtg ccgagtcggg
       61 ttttaaagcc aacataccct ggggtaatgc cactgcaact ttaagatgga accatatttt
      121 taataataaa ctttttgcaa atacatcatt aatatatacc gattatgact tttcgtttgg
      181 tgccggtcag gaggatttta ggtttgaact ttatagcggt gtgcgcgatt ggaatgccaa
      241 agttgatttt aactactacc caagtgtaaa acatacggtt aagtttggtt tcaattatgt
      301 aaaccatttg tttacacctt ctacagcatc ggcaagtcag aacgaaaccg tttttgattt
      361 gggcaaacct tataaaattt atgcaaatga ttttgcactt tatgcattgg acgactgggc
      421 aataaacgat agattaacta taaacggagg tttgcgcgca acaaggtttg tgcaatacgg
      481 tccgtttgac cgatacatta ccaatagcaa ttatgaggta gtggatacta taacttatgg
      541 caaaggcgat aaagttgcgc aatacaacaa tttagagccc agattttcgc ttagcttttt
      601 aattaataaa aacacttcgg ttaaagctgc atacacacaa aattaccaat atgtacattt
      661 ggcttctatt tcttcacttt cattaccaac cgatgtttgg gtgcccagca gcgaccgtgt
      721 aaaaccacag tttgccacgc aatatgctgt aggatttttt aaaaatttta aaaaagactt
      781 gtacgaaaca tctgttgagg tttactataa ggacatgcgc aatcaaattg aatatcgtga
      841 tggtgcaaca cctgatatgg atataaaaaa taatcaggat aacaattttg tttttggtaa
      901 aggctggagc tatggtgccg agttttttgt aaagaaaaaa tatggaaaat ttaatggatg
      961 gattggttac actttggcat ggaccttacg caattttccg gatctaaatc aaggcaaaga
     1021 tttttatgcc agatatgaca ggcgtaacga tttaaaagtt gtagccattt atgagcttaa
     1081 taagaaatgg agctttggtg ccacttttgt ttatgcaacc ggtaatgccc ttaccctacc
     1141 ccagcaacgc tatttatttg gtggcgccat tgatccaaac aacctaaaca cttcgcaatt
     1201 taataaccct caaatttatt acacgtat
//
LOCUS       DAOMAA010000820         1226 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_146288_length_1226_cov_3.309991, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000820 DAOMAA010000000
VERSION     DAOMAA010000820.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146288_length_1226_cov_3.309991"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1226)
                     /gene="sprA"
                     /locus_tag="PLO59_09980"
     CDS             complement(<1..>1226)
                     /gene="sprA"
                     /locus_tag="PLO59_09980"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HQV01478.1"
                     /translation="MDRQYGEQLLRNNTSYPSAIDTTFNKFWNWTRNYDLKWDLTKSL
                     KFDFNAANISRVDEPNGRIDNSEERDIIRKNIYKGGRTVDYRQSGNLSYQVPLNKIPL
                     LDWTSLNVRYAFDYSWLAAPSVFDSTTRSFKPNPILQNTIQNSNNKQANLTLTMTQLY
                     NKIPFFKRLTSNAPPAPKPKPEPPKSPNDTSKTAKPAVPKAPSEINPIIKFAGKMVLM
                     VKTIGVTFSETNGTALPGFKPRPQILGQDLDWKATDGSNSIAPGFGFGFGDQSDIRVK
                     AARNNWLTTDTLFNGQYARSFTQSFTGRSTLEPFTSCRIELNVQRNFTRNQTESYRIN
                     ANTGEYNAFNSLDGGSFSITTITWRTAFVKNNKDYTNETFTQFTNNRAIISQRLGLLN
                     PYSTGQDSTGFWDGYSG"
BASE COUNT          315 a          235 c          280 g          396 t
ORIGIN      
        1 cccgaatagc catcccaaaa accggtcgag tcttggccgg tagagtatgg gtttagtaaa
       61 cccaatcgtt gcgaaataat ggcgcggttg tttgtaaact gcgtaaacgt ttcattggtg
      121 taatctttat tgttttttac aaatgcagta cgccatgtaa tggttgtaat actaaagcta
      181 cctccatcta aactattaaa ggcattatac tcgcctgtgt tggcattaat gcggtaactc
      241 tcggtttgat tgcgtgtaaa gtttcgttgc acatttaact caatacggca cgatgtaaat
      301 ggttctaacg tactgcggcc tgtaaacgat tgtgtaaaac tacgtgcata ctgtccgtta
      361 aataaagtat cggttgtaag ccaattattg cgcgctgctt ttacacggat atcactttgg
      421 tcgccaaagc caaaaccaaa tccgggtgca atgctattac tgccatcggt tgctttccaa
      481 tccaaatcct gacccaatat ttgtggcctg ggcttaaagc cgggtaaggc tgttccattt
      541 gtttcgctaa aggtaacacc aatggttttt accataagca ccattttgcc tgcaaatttt
      601 attatcggat taatttccga aggtgctttt ggcactgccg gcttagcagt tttactggta
      661 tcgtttggcg attttggcgg ctcgggtttt ggctttggtg ccggtggagc attagatgtt
      721 aggcgtttaa aaaacggaat cttattatac aattgtgtca ttgtaagggt aagattggct
      781 tgcttattat ttgagttctg aatggtgttt tgcagtatcg gattgggttt aaaacttctg
      841 gtagttgagt cgaacacgga tggtgctgcc agccacgagt aatcaaatgc atagcgtacg
      901 tttaacgaag tccagtctaa taacggtatt ttgtttaaag gcacctgata acttaagtta
      961 ccactttggc ggtagtcaac cgtacggcca cctttgtaaa tgtttttacg aataatatcg
     1021 cgctcttctg aattgtcaat acggccattg ggttcgtcaa cacgggatat gttggcagcg
     1081 ttaaaatcaa atttaagcga tttggttaag tcccatttga ggtcgtagtt gcgtgtccag
     1141 ttccaaaatt tattaaaagt agtatcaata gccgatgggt aagaagtgtt gttgcgcaac
     1201 aattgttcgc catactgcct gtccat
//
LOCUS       DAOMAA010000821         1226 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_146323_length_1226_cov_2.755764, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000821 DAOMAA010000000
VERSION     DAOMAA010000821.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146323_length_1226_cov_2.755764"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1226
                     /locus_tag="PLO59_09985"
     CDS             <1..>1226
                     /locus_tag="PLO59_09985"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HQV01479.1"
                     /translation="DPYLASAMAVAYVSAFEKNNIITTPKHFVANVGDGGRDSYPVDY
                     SKRHMLEYDFIPFKSCFDFGKSRSVMTAYNALNGKSCSTNKVLLQQILKDEWKFKGFV
                     ISDAGAVGGANVLLNTTKNYTASGKEAIAAGLDVIFQTSVTHEQLFNEPFLSGEMDTA
                     MDKAVTRVLTAKFELGLFDNITEATIPPSHPKDADTLAFEAAVKSLVLLQNNGILPLT
                     QKQKVAVIGTDAIEKRMGGYSGNNNYIVSILDGIKSKSPNVAFSAGPGRGDNFQVIGS
                     EYFIGANNENVKALYYNNISWQGQPVMQFESNLNHHCTFMPPAKNISNTFYSVKWMAS
                     LKAPFTGLCNMGLRGNDGYTLSVNGKPHISRTSKISFHNDVKPYNFIKDSLYLIEVTF
                     YESVGDGSVALVWDAF"
BASE COUNT          397 a          207 c          247 g          375 t
ORIGIN      
        1 aagaccctta cttagcttcg gcaatggctg tagcgtatgt aagtgccttt gaaaagaata
       61 atataattac cacacccaaa cattttgttg ctaatgttgg cgatggtggc cgcgatagtt
      121 atccagttga ttacagtaaa cgacacatgc ttgaatatga ttttattccg tttaaaagct
      181 gtttcgattt tggaaaatca cgctcggtaa tgacagctta taatgcatta aacggcaaat
      241 cgtgtagtac caataaagta ttgttgcaac aaattttaaa agacgaatgg aagtttaaag
      301 gttttgtaat ttctgatgca ggtgctgtag gcggtgccaa tgttttactt aatacaacta
      361 aaaactatac tgcatcaggt aaagaggcca tagcggctgg tttagatgta atttttcaaa
      421 cttcggtaac tcatgaacaa ttgtttaacg agccattttt aagtggcgaa atggatactg
      481 caatggacaa agcagtaaca cgtgtgttaa ctgcaaaatt tgaactgggg ttgtttgata
      541 atataacaga agcaaccata ccaccatccc atcctaaaga tgccgataca ttagcttttg
      601 aagcagccgt taaatcgtta gtgttgctac aaaataacgg catcttacct ttaactcaaa
      661 aacaaaaggt agcagtaatt ggtaccgatg ctattgaaaa aagaatgggc ggatatagtg
      721 gaaataataa ttacatcgtt tcaattttag atggcatcaa aagcaaatcg cccaatgttg
      781 cattttctgc cggaccaggc cgtggcgata attttcaagt tatcggttcc gagtatttta
      841 tcggtgcaaa caacgaaaat gtaaaagcgc tttactacaa taatataagt tggcaagggc
      901 agcccgttat gcagtttgaa tcgaacctta atcaccattg tacgtttatg cctcctgcaa
      961 aaaacatatc caatactttt tattccgtaa aatggatggc cagtctaaaa gcacctttta
     1021 caggtttgtg caatatggga ttaagaggta atgatggtta cacgctttct gttaatggta
     1081 agccacatat aagtcgtaca agcaaaatta gttttcataa tgatgtaaag ccatataact
     1141 ttattaaaga cagtttgtat ttaattgaag ttacatttta cgaatcggtt ggcgatggca
     1201 gtgttgcatt agtctgggat gctttt
//
LOCUS       DAOMAA010000822         1226 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_146394_length_1226_cov_2.188728, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000822 DAOMAA010000000
VERSION     DAOMAA010000822.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146394_length_1226_cov_2.188728"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="PLO59_09990"
     CDS             complement(<1..368)
                     /locus_tag="PLO59_09990"
                     /inference="COORDINATES: protein motif:HMM:NF037329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
                     /protein_id="HQV01480.1"
                     /translation="MSLKGIISIAKMPGLYKVIAQSKSGFIVESLGDKKRMPVSATQR
                     ISMLEDISMYCKSGDTPLKDIFQKMKNSEAILSLDAKADIEKLKAAFETVLPDYDTER
                     VYNSDIQKVIKWFQMLKDEV"
     gene            complement(462..>1226)
                     /locus_tag="PLO59_09995"
     CDS             complement(462..>1226)
                     /locus_tag="PLO59_09995"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV01481.1"
                     /translation="KYVIVEYVIKNNAASALNNVRAGLCFDWDIMDYTQNRSSENASI
                     RLGYSFNTQVGGLYAGVKVLTSGGFNHYAIDNISGGGGGLDISGGFTTAGKFTTLSTA
                     RAAAGTNGTGNDVIDIVATGPYNIAAGDSIKIAFAILAGTELADLENSANSAQIKYDG
                     LTAIKDLDNKMQLQLMPNPANDLVNLGFTVVRPGAVNISIYNANGKLVKQTGNVYSMG
                     YVNESINTSQLSNGIYMIVVKQGNSTTSTKLTIMHQ"
BASE COUNT          352 a          250 c          193 g          431 t
ORIGIN      
        1 cttacttcat cttttagcat ctgaaaccat ttaattacct tttgaatatc gctgttgtaa
       61 acgcgctcgg tatcataatc gggcaatacg gtttcgaatg cagcttttaa tttttcgata
      121 tcagctttgg catctaaact taaaatcgct tcactatttt tcattttctg aaaaatatcc
      181 tttagtggtg tatcgccact tttacaatac atacttatgt cttctaacat actgatgcgt
      241 tgtgttgcac taacgggcat gcgcttttta tcgcctaatg actcaacaat aaatccgctt
      301 ttcgattgtg caattacttt atacaaaccg ggcattttag caatggaaat aattcctttc
      361 aaactcataa taacttgctt ttatttttaa tattaaatct ttctgaattg ataattttat
      421 aaaccagcgg tcaaaattaa taattgactt caaatcgtaa actattgatg cattatggta
      481 agttttgtgc tggttgtact atttccttgt ttaacaacaa tcatataaat accgttgcta
      541 agttggcttg tatttattga ctcattaaca taacccatac tatatacgtt acctgtttgc
      601 tttaccagct tgccatttgc attataaata ctaatgttaa ctgcacctgg cctgactact
      661 gtaaacccta agtttaccaa atcattggct gggtttggca taagttgtaa ttgcattttg
      721 ttatccaaat ctttaatggc agttaagcca tcatatttta tttgtgcact gttggcgctg
      781 ttttccaagt cggccaattc agtaccggca agtatggcaa atgcaatttt tatactgtcg
      841 cctgcagcaa tgttataggg tccggtggca acgatatcaa taacatcatt gccggtacca
      901 ttagtacctg cagcagccct tgctgtacta agtgttgtaa atttaccggc tgttgtaaaa
      961 ccgcccgata tgtctaaacc accacctcca ccagaaatgt tatcaatagc ataatggtta
     1021 aaaccaccac tggttaaaac ttttactccg gcatacaatc cgccaacttg tgtattaaat
     1081 gaatagccta agcgtatgct tgcattttcg gacgaacggt tttgggtata atccataata
     1141 tcccaatcaa aacacaaacc ggcacgtacg ttatttagtg ctgatgcggc attgtttttt
     1201 attacatatt caacaattac atactt
//
LOCUS       DAOMAA010000823         1224 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_146785_length_1224_cov_2.741660, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000823 DAOMAA010000000
VERSION     DAOMAA010000823.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146785_length_1224_cov_2.741660"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1224)
                     /locus_tag="PLO59_10000"
     CDS             complement(<1..>1224)
                     /locus_tag="PLO59_10000"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HQV01482.1"
                     /translation="AVGAMPGDTLNNMSPNNLLVTTIGDELHISNNFKNKVIGVAIKD
                     RGAILPAGRSANAAYWWDWKSKNFITSTYYMQALPNWVKEFNAQKLPDTYFKEGWKLK
                     NAGSSYSSSTADNTPYEGLFKGEKAPVFPHLIADSNTIDALKFMPQGNDLTLQFAMAA
                     INNENLGNNGLTDMITISFSSTDYAGHRYGTHAVEIEDMYIKLDENIHTLIDFLERKI
                     GKDKILLFLTADHAAIPNAQYLADHHINAGYFNFKQFASALRADVQLNHGDSNLIEKF
                     DNDMIYINRSLLKNKNQLNDVTATIYDFCLHYKGISHALTAAQIQNNVYTEGAKSLMQ
                     KGFHTQKCGDLVYQLDAGLVEYGKTGTTHGSAYPYDTHVPVWFYGSKVAPGVCNRKTS
                     ITDIAPTICNLLQIER"
BASE COUNT          336 a          229 c          234 g          425 t
ORIGIN      
        1 cgttcaattt gtagtaaatt acaaattgta ggtgcaatat ctgtaatgct tgttttacgg
       61 ttacaaaccc ccggagcaac tttacttcca taaaaccata cgggtacatg tgtatcatag
      121 ggatatgccg aaccgtgtgt ggtacctgtt ttgccatact caaccaaacc ggcatctaac
      181 tgataaacca aatcgccaca tttttgtgta tgaaagcctt tttgcatcag cgatttagca
      241 ccttcggtat atacattatt ttgaatttgt gcagcagtta atgcatggct aatgcctttg
      301 tagtgcaaac aaaagtcata tatagtagct gttacatcgt ttaattgatt tttgtttttt
      361 aataaactac ggttgatata tatcatatcg ttatcaaact tttctatcag gttactatcg
      421 ccatggttta gttgcacatc tgctcttaat gccgatgcaa attgtttaaa gttaaaatag
      481 ccagcattaa tatgatggtc ggctaagtat tgggcattgg gtattgcagc atgatcggct
      541 gttagaaaaa gcaatatttt atccttgcca attttacgtt ctaaaaaatc aatcagtgtg
      601 tgtatatttt catcaagttt tatatacata tcttctatct caacagcatg tgtgccatag
      661 cgatggcctg cataatctgt gctcgaaaaa ctgatggtta tcatatcggt aaggccgttg
      721 tttcctaaat tttcattatt aatggcagcc attgcaaact gcaatgtaag gtcattgccc
      781 tgtggcataa actttagtgc atcaatggta tttgagtcgg ctataaggtg tggaaacaca
      841 ggtgcttttt cgcccttaaa taatccttca taaggggtat tatcagccgt gcttgaggag
      901 taggatgagc ctgcgttttt taatttccaa ccttctttaa aataagtatc aggcaatttt
      961 tgtgcgttaa attcttttac ccaattgggt agtgcttgca tgtagtaagt actggttata
     1021 aagtttttgc ttttccaatc ccaccaataa gctgcattgg cactacgacc ggccggcagt
     1081 atggcacctc tgtcttttat ggcaacacct attactttgt ttttaaaatt gttgctgata
     1141 tgtaactcat caccaatggt ggtaaccaat aaattgtttg gcgacatgtt gtttaatgtg
     1201 tcaccgggca ttgctcccac agct
//
LOCUS       DAOMAA010000824         1221 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_147630_length_1221_cov_1.791595, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000824 DAOMAA010000000
VERSION     DAOMAA010000824.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147630_length_1221_cov_1.791595"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1129)
                     /locus_tag="PLO59_10005"
     CDS             complement(<1..1129)
                     /locus_tag="PLO59_10005"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HQV01483.1"
                     /translation="MRALFKWYANQMFYKKQALKTLAIIVCISFFAPVICNETPLILK
                     TELGCSSPLFSKWFYGTLPQNCSSVYVNNTWQYQHAIFVLLPPIPYSANFYDYNNAPF
                     ISPFQKQSDGLNNTELSWRYRHWLGTGKKGEDVLACCIYGLANSIFISGAATLLAALI
                     AFVLGITSGFFGNNYFKLSWLHYLGLVGGFLSGYYFMVLLFPLWFDFNNLIFAGAFIA
                     IWLIVFLVFSQLAKRFSVSGSLNFPYARIIHFFVNIFSAIPALILIVVVIAWLKPTVT
                     TLIILLAITGWPTLMRIAYIETKQIMTKPYMLTATAMGYSADYIIRKEILPNFSNAFA
                     TTTIYVFAALLVAESSLTFLGLGTPYPFVTLGSLLGQFNENF"
BASE COUNT          464 a          212 c          204 g          341 t
ORIGIN      
        1 caaaattttc attaaactga cctaacaaac tgcctaaagt tacgaacggg tagggtgtac
       61 ctaaacctaa aaaagttaat gaagactcgg ctaccaataa tgcggcaaat acataaatgg
      121 ttgtagttgc aaaggcgttt gaaaagttag ggagaatttc ttttctgatt atataatcag
      181 cactatagcc cattgctgtt gcagtgagca tataaggttt ggtcattatt tgttttgttt
      241 cgatatacgc tattcgcatt agcgtaggcc aacctgtaat tgccagtaat ataatcagtg
      301 tagtaacggt tggttttagc catgcaatta caacaactat taaaattaaa gccggaatag
      361 cagaaaatat attcacaaaa aaatgaatga ttctagcata agggaaattt aaacttcccg
      421 aaaccgaaaa ccgtttagct aattgactaa atactaaaaa aacaataagc caaattgcaa
      481 taaatgcgcc tgcaaaaatt aaattattaa aatcaaacca taacggaaaa agcaatacca
      541 taaaataata gcctgataaa aatccgccaa ccaggcctaa atagtgcaac catgaaagtt
      601 taaaatagtt attaccaaaa aatccggatg taatgcctaa aacaaaagca attaaagcgg
      661 ctaataaagt tgccgcacca ctaataaaaa ttgaattggc taatccataa atgcagcaag
      721 ccaacacgtc ttcgcccttt ttgccggtgc ctaaccagtg cctgtaacgc catgaaagtt
      781 ccgtattatt taaaccgtca ctttgctttt gaaaaggtga aataaaaggt gcattgttat
      841 aatcgtaaaa gttggcacta taaggtatgg gtggcagcaa tacaaaaatt gcatgctgat
      901 actgccacgt attatttaca taaaccgagc tgcaattttg tggcaacgta ccataaaacc
      961 atttactaaa aagcggtgaa ctacaaccaa gttctgtttt taaaataaga ggtgtttcgt
     1021 tacaaattac gggtgcgaaa aaactgatac aaacaattat agcaagtgtt tttaaagctt
     1081 gcttcttata aaacatctga ttggcatacc atttaaaaag tgctctcaaa tgtgtgattt
     1141 taaattaggg ttaattaatt tttgaataac atcaactaat gtagcagcaa aaatagtagt
     1201 tacaccactt attaaaacca a
//
LOCUS       DAOMAA010000825         1220 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_147776_length_1220_cov_2.498712, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000825 DAOMAA010000000
VERSION     DAOMAA010000825.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147776_length_1220_cov_2.498712"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /gene="pdxA"
                     /locus_tag="PLO59_10010"
     CDS             complement(<1..993)
                     /gene="pdxA"
                     /locus_tag="PLO59_10010"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HQV01484.1"
                     /translation="MTTENAPILKVGISIGDTNGIGFEVIMKTFSDSRMHQICTPVVY
                     GSAKTASFQRKALNQPDFAYQTIKSNADIIDRKINLINLWDEEIKTDFGVANDIGGKY
                     AFKSLQAAVTDVKQQLVDVLVTAPINKKNIQQEGFNFAGHTEYLAQETQSKNYCMMLV
                     SDAMRIAFVTGHVPLHKVATQITTDTIIAKLRVMHESLKRDFGIRKPRIAVLGLNPHA
                     GDDGLIGNEENNIITPAINEARHAGFLLAGPFSADGFFGAGQHQKFDGILSMYHDQGL
                     IPFKALSFSSGVNFTAGLPIVRTSPDHGTGYDIAGKGIASESSFREAVYLAMDIY"
BASE COUNT          351 a          244 c          233 g          392 t
ORIGIN      
        1 ataaatatcc atagccagat aaaccgcttc gcgaaatgat gactcgctgg caatgccctt
       61 acctgcaatg tcgtaacctg taccatggtc gggcgaggta cgtacaatag gcaagcctgc
      121 tgtaaagttt acaccactgc taaacgaaag tgccttaaag ggtataagcc cttgatcgtg
      181 gtacatgctt aatatgccgt caaatttttg atgttgcccg gcaccaaaaa aaccatcggc
      241 actaaagggc cctgctaata aaaatcctgc atggcgcgct tcgtttatgg ccggtgttat
      301 tatgttgttt tcttcattgc caattaaacc atcgtcaccg gcatgtggat ttaaacctaa
      361 aacggctatg cgtggcttgc gaatgccaaa atcccgtttc aacgattcat gcattacacg
      421 taacttcgca attatggtat cggttgtaat ttgagtggct actttatgca atggcacgtg
      481 tcctgtaaca aatgcaatac gcatggcatc gcttaccaac atcatacaat aatttttgct
      541 ttgcgtttcc tgcgccaaat actcggtatg gccggcaaaa ttaaaaccct cttgttgtat
      601 gtttttttta ttgatgggtg ctgttaccag tacatcaacc aattgctgct taacatcggt
      661 tacagcggct tgcagcgatt taaaagcata cttgcctcca atatcattag ccacaccaaa
      721 atcagtttta atttcttcgt cccacaggtt aatcaggtta atttttctgt caattatatc
      781 ggcattactt ttaatggttt gatatgcaaa atcgggttgg tttaatgctt tgcgttggaa
      841 gctggctgtt ttggccgagc catatactac gggcgtacaa atttgatgca tgcggctatc
      901 gctaaaggtt ttcataataa cttcgaagcc aatgccattg gtatcgccaa tgcttatgcc
      961 tacttttaat atgggtgcgt tttcagttgt cattttaaaa tggtgccaag cttatgcttg
     1021 tctaaattta agtgtactat aatgtaatga aatattaata atttgatgtg aaattattaa
     1081 gacatcaggc ggcaaagtta atttttgaat gtaattaata tgcaacgcca ttttattgct
     1141 tttctttggc aaatcttata aaatacaact tacttaaatt ttaaaaatca aaaaaattat
     1201 ggtacgtaaa gcaacagcaa
//
LOCUS       DAOMAA010000826         1220 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_147895_length_1220_cov_1.512446, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000826 DAOMAA010000000
VERSION     DAOMAA010000826.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147895_length_1220_cov_1.512446"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="PLO59_10015"
     CDS             <1..248
                     /locus_tag="PLO59_10015"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV01485.1"
                     /translation="ALQAYPNPFTETLEIKSFALLTNTAFLLEIYDVSGRIINTPQLI
                     TNHVVIETSGWSPGFYTARVYDAHSSTYRSIQLVKTP"
     gene            257..>1220
                     /locus_tag="PLO59_10020"
     CDS             257..>1220
                     /locus_tag="PLO59_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01486.1"
                     /translation="MKKQLLATLLITVTLCITSMAQPALNWVNGLTGTNTNSTTNNSN
                     TVTTDATGNVFIAGYFTGTIDFDPGTGVSNLTAQGAYADMFVAKYAPNGSLQWAKQVR
                     THDAYPPNEATAVNMRLTNTGEPVITGWLDGVQDLNPDAGADTFGTTIGLSLFLLKLD
                     NNGNYIWAKAISAYGFPKISIDNANNIYLAGWYQGSAEDFNPGPGVDSLPRSLYTDIF
                     IAKYDANGNFAWVKGLHSGASEIINDIVNDAAGNIYVCGNFEGSVLDFDPDTSTAERY
                     RQSLGAGTDFFIAKYTTNGGLLWANTYGHPTYINSSNTADRLMLD"
BASE COUNT          358 a          289 c          256 g          317 t
ORIGIN      
        1 ttgccctaca ggcatatcca aacccgttta cagaaacttt agagattaag agtttcgcat
       61 tactgacaaa cacagctttt ttactggaga tatatgatgt aagtggtcga ataattaata
      121 cgccgcagct tataactaat cacgtagtaa ttgaaacatc cggttggtcg cccggatttt
      181 atacagcccg ggtttacgat gcacatagtt ccacttaccg cagcattcaa ttagttaaaa
      241 caccataaac aacaatatga agaaacaact acttgctact cttctaatta ccgtaacact
      301 ttgcattaca tctatggcgc agcctgcatt aaactgggta aatggtttaa caggcactaa
      361 tacaaactca acaaccaaca acagcaatac cgttaccacc gatgcaaccg gcaatgtttt
      421 tattgccggt tattttaccg gcactataga ttttgacccc ggaacgggcg taagcaactt
      481 aaccgcacaa ggagcttatg ccgatatgtt tgttgccaaa tatgcaccta atggaagttt
      541 gcaatgggcc aaacaagtgc gaacgcacga tgcctacccg cccaacgagg ctacggcagt
      601 aaacatgcga ctaaccaata ccggcgagcc cgttataacc ggttggcttg atggagtgca
      661 ggacttaaac ccagatgcag gagcagatac ttttggcacc accatcggcc ttagtttgtt
      721 tttacttaag ctggataata acggcaacta catttgggca aaagcaatct ctgcttacgg
      781 ttttcctaaa atcagtattg ataacgctaa caatatttac ctggcaggtt ggtaccaggg
      841 cagcgccgaa gattttaacc ccggccccgg ggttgatagt ttaccccgta gcctgtatac
      901 tgatattttt atagccaagt acgatgctaa cggaaacttt gcatgggtaa aggggctaca
      961 ctcgggtgcc agcgagatta taaacgacat agtgaatgat gctgcaggca acatatatgt
     1021 atgcggcaac tttgaaggtt cggtccttga ttttgacccc gacaccagta cggcagaacg
     1081 ataccgtcaa tcgctcggtg ccggcaccga tttttttata gccaagtata ccaccaatgg
     1141 cggtttgctt tgggcgaata cgtatggaca tccaacttac ataaactcct ctaacaccgc
     1201 cgatcgttta atgcttgatg
//
LOCUS       DAOMAA010000827         1217 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_148507_length_1217_cov_2.403614, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000827 DAOMAA010000000
VERSION     DAOMAA010000827.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148507_length_1217_cov_2.403614"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..879)
                     /locus_tag="PLO59_10025"
     CDS             complement(64..879)
                     /locus_tag="PLO59_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01487.1"
                     /translation="MKTKIQQLIKKWWATVMWALVLTSAAISFAFMHSKQNHEVCKKL
                     IIQNVDDKNGFIDNADILEMLALKSLNPIGKPINEINTALLENIINSNPYVANTEVFS
                     TIDGKVHVTIRQRNPVVRIINQTGENYYIDDTGYLMPVSPKFTSDVLVANGYINDVYN
                     HKQINTSTDSTTDVLSQIFKVATYLQSDTIRNTLFTQLFVTNNKQLELVPRIGNHIVQ
                     IGNASALPEKINKLMVFYVKGLNKTGWDTYSTINVQYHSQVVCTRKQELDKQQ"
     gene            complement(869..>1217)
                     /locus_tag="PLO59_10030"
     CDS             complement(869..>1217)
                     /locus_tag="PLO59_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HQV01488.1"
                     /translation="LSAQELNSGKKITVIFQPHLFTRTRDFADAFAAVLSLVQQVILL
                     PIYPARELPIDGVTSQMLADKISCNVQLCEKENLLQVLQTKQLQVLLTLGAGDIDQLV
                     NPISQFLSQRYEN"
BASE COUNT          353 a          213 c          216 g          435 t
ORIGIN      
        1 caactatgat atttgattga ttcatatttt taatattact aacgtaggtt atatactata
       61 taattattgt tgtttgtcaa gttcttgttt ccgcgtacat actacctgac tgtggtattg
      121 tacgttaatg gtactgtagg tatcccatcc ggttttattt aatcctttta cataaaacac
      181 cattagctta tttatttttt cgggcagtgc acttgcatta ccaatttgaa ctatgtgatt
      241 gccaatacgc ggtactaatt ccaactgttt gttatttgtt acaaatagtt gcgtaaacag
      301 ggtgttacgt atggtatcgc tttgcagata agtagctacc ttaaatattt gcgacaacac
      361 atcagtagta ctatctgttg aagtattaat ttgtttatgg ttgtaaacat cattaatgta
      421 accgttagca accaaaacat ctgaagtaaa ttttggcgat acaggcatca gatagcctgt
      481 atcatcaatg taatagtttt cgcctgtttg attaatgata cgcaccaccg gattgcgttg
      541 ccttattgta acatgaacct tgccgtcaat ggttgaaaat acttcggtgt tagcaacgta
      601 aggattacta ttgatgatat tttctaacaa agccgtgtta atttcattta taggtttacc
      661 aatagggttt aaacttttta gtgcaagcat ttcaagtata tctgcattgt ctataaaacc
      721 atttttatca tccacattct gaataatcag ttttttacaa acttcatggt tttgcttgct
      781 gtgcataaat gcaaacgata ttgctgcact tgtcaacacc aaagcccaca ttacggttgc
      841 ccaccatttt tttatcaact gttgtatttt agttttcata gcgttgtgat aaaaattgtg
      901 aaatcggatt aaccaattga tcaatatcac ccgcacctaa ggttagtaaa acttgcaatt
      961 gtttggtttg caaaacttgc agcagatttt ctttttcaca aagttgtacg ttacacgata
     1021 ttttatcagc cagcatttgt gatgttacgc catcgatagg cagctcgcgt gccggataaa
     1081 ttggaagcag aattacctgc tgcactaaac taagtacagc agcaaatgca tcagcaaaat
     1141 cgcgtgtgcg tgtaaataaa tggggttgaa atattacggt tatttttttt ccggagttga
     1201 gttcctgagc tgataaa
//
LOCUS       DAOMAA010000828         1216 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_148655_length_1216_cov_3.313523, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000828 DAOMAA010000000
VERSION     DAOMAA010000828.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148655_length_1216_cov_3.313523"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="PLO59_10035"
     CDS             <1..261
                     /locus_tag="PLO59_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01489.1"
                     /translation="PENFFAEGSNVNSSNWNLYHLTLGFDVDIKKVKVSVGLKCSYGA
                     SKNNLQFVDISTAHAGNLLYGNRKYISNYNNIHGGIILGLNF"
     gene            378..>1216
                     /locus_tag="PLO59_10040"
     CDS             378..>1216
                     /locus_tag="PLO59_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01490.1"
                     /translation="MMRYFLVWVYCFLSLYSWAQSNIKKQTAEEKCLLLNQIMLDSSN
                     AFKLSAALRLADSLELVAGKANLPCYKIEAYLMQANVFSKRQQFDAAKVSFAKAYTQL
                     HYCNNKLQYANYYRAFALDQSIQNKHQQAVNNYNKAITYLGYNSKQIVVAKVFYKIGY
                     CFYNLQSYDSALINLTNAIAIAKQLSDTTSLFETYRLMAHCYASMGLENKAFEVAQMA
                     SYYDNTKISAIQKRSFYYETAFVAICLDSIEVAEKFSALFYNCNKKFDDDFGKQCEAK
                     LKG"
BASE COUNT          431 a          195 c          211 g          379 t
ORIGIN      
        1 cccgaaaatt tttttgccga aggcagcaat gtaaacagca gcaattggaa tttgtatcat
       61 ttaactttag gctttgatgt tgatattaaa aaagtaaaag tttcggtagg tcttaagtgc
      121 tcatacggtg ccagtaaaaa caatttacag tttgtagata taagcacagc gcatgcaggt
      181 aacttattat acggtaacag aaaatacata agcaactaca acaatataca tggtgggatt
      241 attttgggtt taaattttta atcaattttc aaagcaagta tggatagttg acatctgctt
      301 gataatttga aacagcggta tcaaaacttc attaagcaat atatatttac acataacaac
      361 ctttctgatt ataagttatg atgcgttatt ttttagtatg ggtatattgc tttttaagcc
      421 tctatagttg ggcgcaaagc aatattaaaa aacaaacagc tgaagaaaag tgtttgctgc
      481 ttaaccaaat tatgctcgat agcagcaatg cttttaaact aagtgcagca ctcaggttag
      541 ccgatagttt ggaattagtt gctgggaaag ccaacctgcc atgttataaa attgaagctt
      601 atttaatgca agccaatgta tttagcaaac ggcaacagtt tgatgccgct aaagtatcgt
      661 ttgcaaaggc ttatacacaa cttcactact gtaacaacaa actacaatat gccaattact
      721 accgcgcatt tgcattagat caaagtatcc agaacaaaca tcaacaagct gtaaataatt
      781 acaacaaagc cattacatac ttaggttaca atagtaaaca gattgtagtg gctaaagtgt
      841 tttataaaat agggtattgc ttttataatc tccaatccta tgatagtgca ttaattaacc
      901 taacaaatgc aatagcaata gcaaaacaac ttagcgacac aacttcttta ttcgaaacct
      961 atagactaat ggctcattgt tacgcgagta tggggttgga aaataaagca tttgaagttg
     1021 cccaaatggc ttcttactat gataatacta aaatttcagc tattcaaaaa cgttcatttt
     1081 attatgaaac tgcatttgtt gctatttgtt tagatagcat tgaagtagcc gaaaagtttt
     1141 cagcgttatt ttataactgt aataaaaaat ttgacgatga ttttggcaaa caatgcgagg
     1201 ccaagctaaa gggctt
//
LOCUS       DAOMAA010000829         1216 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_148713_length_1216_cov_2.534884, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000829 DAOMAA010000000
VERSION     DAOMAA010000829.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148713_length_1216_cov_2.534884"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1216
                     /locus_tag="PLO59_10045"
     CDS             <1..>1216
                     /locus_tag="PLO59_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018341678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQV01491.1"
                     /translation="TDDGKHYVLNGQKMWITNAGFANVFTVFAQVDGNKFTAFIVESG
                     TAGLSLGDEEHKMGIKGSSTRQVFFADCKVPVENVLGEVGKGHLIAFNILNIGRIKLA
                     IAALGGAKRVSTMSVQYAATREQFKLPIAKFGAIRYKLAEQAIRLFACESSIYRVADL
                     IDEKERALLAEGKNYADALLGAAEEYAVECAMLKVSGSEVLDYIVDEGVQIYGGYGYS
                     ADFPMDRAYRDSRINRIFEGTNEINRLLTVDMLLKRAMKGELDLMTPAMAVQKELLEI
                     PDFTEDETPFAAYYKLIANFKKAILMVAGTAVQKYMMQLAKEQEILINIADMAIDAYN
                     AESLLMRVQKITDMKGDEAAATYNAMLQVYLFDMADKIHNSGKNAIASIAEGDELRLL
                     LLGLKRFTKTAPV"
BASE COUNT          378 a          216 c          285 g          337 t
ORIGIN      
        1 ttaccgatga tggtaaacat tatgtgttga atggccaaaa aatgtggatt accaatgcag
       61 gcttcgcaaa tgtgtttacc gtgtttgcac aggttgatgg aaataagttt acagctttta
      121 tagttgagtc gggtacagcc ggattatctt taggcgatga agaacataaa atgggtatta
      181 aaggcagctc tacccgccag gtgttttttg ccgattgcaa agtgcctgtc gaaaacgttt
      241 taggcgaagt aggcaaagga catttaattg cttttaatat tctaaacatc ggtcgaatta
      301 aattagccat tgcagcatta ggtggcgcaa agcgggtttc taccatgagt gtgcaatatg
      361 ctgcaacgcg cgaacagttt aaattgccaa ttgcaaagtt tggtgctatc agatacaaat
      421 tagccgaaca agccataaga ctgtttgctt gcgagtcgag catctaccgt gtagccgatt
      481 taattgatga aaaagaacgt gccctgctag ccgaaggtaa aaattatgcc gatgctttgt
      541 taggcgctgc cgaagaatat gccgttgagt gtgccatgct taaagttagt ggtagcgaag
      601 ttttagatta tatagttgac gaaggcgtac aaatttatgg aggatatgga tacagtgccg
      661 attttccgat ggaccgcgca taccgcgact cgcgcattaa ccgcattttt gaaggtacca
      721 atgaaattaa caggttgctt actgttgata tgctgttaaa gcgcgccatg aaaggcgagt
      781 tggatttaat gacacctgca atggctgtgc aaaaagaatt gctcgaaata cctgatttta
      841 cggaagatga aacacctttt gcagcatact ataagcttat agccaacttt aaaaaagcaa
      901 tattgatggt agcgggtaca gccgttcaaa aatacatgat gcaattggct aaggagcagg
      961 aaatacttat aaatatagcc gatatggcca tagacgccta taatgccgaa tcgttattaa
     1021 tgcgcgtgca aaaaataacc gatatgaagg gtgatgaagc ggcagcaacc tataatgcca
     1081 tgttacaggt ttacttattc gatatggccg ataaaattca taattcaggt aaaaatgcaa
     1141 ttgcatcaat agccgaaggt gatgagttgc gtttattact cttaggcctc aaacgtttta
     1201 ctaaaacagc accggt
//
LOCUS       DAOMAA010000830         1215 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_148868_length_1215_cov_5.121552, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000830 DAOMAA010000000
VERSION     DAOMAA010000830.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148868_length_1215_cov_5.121552"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..672
                     /gene="gmk"
                     /locus_tag="PLO59_10050"
     CDS             94..672
                     /gene="gmk"
                     /locus_tag="PLO59_10050"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013926782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HQV01492.1"
                     /translation="MPNKLILFCGPSGSGKTTIVKYLLANSTRLTFSVSATTRQKRTT
                     EIDGKDYHFISVDDFKKHISNNDFIEWEEVYTNGYYGTLKADVDAHLKNNKVVLFDVD
                     VEGGLNIKKYFGENLLAVFVQPPSVIELEHRLIARQSESAESLKKRLAKASLELTYAN
                     RFDKVLINDNLETALWHAQKMVEDFTGFSLNA"
     gene            685..>1215
                     /gene="nadD"
                     /locus_tag="PLO59_10055"
     CDS             685..>1215
                     /gene="nadD"
                     /locus_tag="PLO59_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HQV01493.1"
                     /translation="MKIGLYFGSFNPVHNGHMVIANYMAEFAQLDQVWMVVSPQNPLK
                     APTTLLSDTQRLAMVKIAIGDYKKLKASNIEFKLPKPSYTIHTLVYLSEKYPQHEFAL
                     IMGADSLATLPKWKNHEAILEHYHIYVYPRPQITLPALANNKHVHIVNAPLMELSSSF
                     IRNAIKAKHDVRFMVPH"
BASE COUNT          418 a          238 c          227 g          332 t
ORIGIN      
        1 tgtagtagaa atgaaagacg agctcgaaaa aatgaaagaa caaatagcca acgttttata
       61 acaagtaaca taacctatat acgccataca accatgccaa acaaactcat acttttttgt
      121 ggccccagtg gcagtggtaa aaccaccatc gttaaatatc ttttagccaa cagcacacgg
      181 cttacatttt cggtaagtgc tacaacacgc caaaagcgaa ctactgaaat tgatggtaag
      241 gattatcatt ttataagtgt tgatgacttt aagaaacaca tcagcaacaa cgattttatt
      301 gaatgggaag aagtttatac caatggatat tatggcactt taaaggcaga tgttgatgca
      361 caccttaaaa acaataaagt ggttttgttt gatgtagatg tagagggcgg cttaaacatt
      421 aaaaaatatt ttggtgaaaa cctgctggct gtatttgtgc agccgccatc ggtaatcgag
      481 ctcgaacacc gtttgatagc acgccaatcc gaatcggccg aatcgctgaa aaaaagattg
      541 gccaaagcaa gtctggagct aacctatgcc aaccggttcg acaaggtttt gataaacgat
      601 aatttggaaa cagctttatg gcatgcacaa aaaatggtgg aagattttac agggttttca
      661 ctcaatgcat aatgcaaacc tcaaatgaaa attggtttat actttggttc atttaatccg
      721 gtacataacg gccacatggt tattgcaaac tacatggccg agtttgcaca gttagaccag
      781 gtatggatgg tagtatcgcc acaaaaccca cttaaagcac caactacttt actaagcgat
      841 acgcaacgtc tggcaatggt taaaattgct attggcgact acaaaaaact gaaagcttct
      901 aacattgaat ttaagttgcc caaaccatcg tacaccatac ataccttggt gtacctttca
      961 gaaaaatatc cacaacacga gtttgcttta attatgggtg ccgatagttt ggcaacatta
     1021 ccaaaatgga aaaaccatga agccatttta gaacattatc atatttatgt ttatccacgt
     1081 ccgcaaatta ctttaccggc attagcaaat aataagcatg tgcatattgt aaacgcaccg
     1141 ttaatggaac tctcatcaag ttttattcgt aatgcaatta aagccaagca tgatgtacgt
     1201 tttatggtac cccat
//
LOCUS       DAOMAA010000831         1215 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_148919_length_1215_cov_2.790517, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000831 DAOMAA010000000
VERSION     DAOMAA010000831.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148919_length_1215_cov_2.790517"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1215)
                     /locus_tag="PLO59_10060"
     CDS             complement(<1..>1215)
                     /locus_tag="PLO59_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HQV01494.1"
                     /translation="QEGLGEVQEMIDICDFAVGLSRQLHGYTMHSERPLHRMYEQYHP
                     LGIVGIISAFNFPVAVWSWNAMLAMVCGDCSIWKPSSKTMLCAIAVHNIIAKVLADNK
                     VPEGVLCLIAAGSKYVGDNFLEDKRIPLISATGSTRVGKRVGRIVGERLGRSLLELGG
                     NNAIIISQHANQDMALRATVFGAIGTAGQRCTSTRRLIIHSSIYESFKQKLIKAYSGA
                     TIGHPLNPTTLVGPLIDTGAVNDFTKAIAQVTTEGGKVIYGGTVCTGAGFESGCYVVP
                     CIVEAQNHYHMVQEETFAPILYIMKYDTIEEAISLQNSVPQGLSSSIFSNHVIETESF
                     LSHAGSDCGIANVNIGTSGAEIGGAFGGEKETGGGRESGSDSWKIYTRRQTNTINYGS
                     SLPLAQGIKFDI"
BASE COUNT          327 a          298 c          249 g          341 t
ORIGIN      
        1 taaatatcaa acttaatgcc ttgtgccaat ggcaaactgc tgccatagtt tatggtattg
       61 gtttgcctgc gcgtataaat tttccacgag tcgctaccgc tttcgcggcc gccaccggtt
      121 tctttttcgc ctccaaaagc gccaccaatt tcggcaccac tggttccaat gtttacattg
      181 gcaatgccac agtcgctgcc tgcatgcgat aaaaagcttt cggtttcgat aacatgatta
      241 ctgaaaatgg atgatgaaag cccttgtggc acactgtttt gcaaacttat ggcctcttca
      301 atagtatcat atttcataat gtataaaata ggcgcaaagg tttcttcctg caccatgtgg
      361 tagtgatttt gtgcctcaac gatgcagggt acaacgtaac agccgctttc aaaaccggca
      421 cccgtgcata ctgtgccacc atatataacc ttgcctcctt ctgttgttac ctgtgcaatg
      481 gctttggtaa aatcgtttac ggcaccggta tcaattaaag gcccaaccaa ggttgtagga
      541 tttagcggat ggccaatagt agcgccactg tatgccttta ttaatttttg tttaaacgat
      601 tcgtaaatgc tgctgtgtat aataagtctg cgcgttgagg tacagcgttg ccctgccgtg
      661 ccaatagcgc caaaaacagt agcacgcaaa gccatatcct gattggcatg ctggctgata
      721 attattgcat tgttaccacc taactctaac aagctgcgac ccaaacgctc gcctacaata
      781 cggcctacac gcttgcccac acgtgttgaa ccggtggcac ttattaatgg gatgcgctta
      841 tcttctaaaa agttatcgcc tacatattta ctgccggcag caataaggca aagcacacct
      901 tcgggtactt tattgtcggc taaaactttg gcaataatat tatgtacagc tatggcacat
      961 aacatggttt ttgaagaggg tttccagata gagcaatcgc cacataccat ggcaagcatg
     1021 gcattccaac tccaaaccgc taccggaaaa ttaaatgcgc ttataatgcc cacaatacct
     1081 aaaggatgat attgttcata catacgatgt aatgggcgct cgctatgcat ggtgtaacca
     1141 tgtaactggc gcgaaaggcc aactgcaaag tcgcatatat ctatcatttc ctgcacctcg
     1201 cctaagccct cttgt
//
LOCUS       DAOMAA010000832         1215 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_148958_length_1215_cov_2.405172, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000832 DAOMAA010000000
VERSION     DAOMAA010000832.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148958_length_1215_cov_2.405172"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..703)
                     /locus_tag="PLO59_10065"
     CDS             complement(137..703)
                     /locus_tag="PLO59_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HQV01495.1"
                     /translation="MKILLLVNYDSFTYNLYHYLQQLTSFQIDVIRNDEIYLNEIEIY
                     QSIVLSPGPGLPNEAGLMMEVIKQYVATKKILGVCLGMQAIVQHFGGTLKNLQTVQHG
                     VSRLTHVMQPDALYKDIPADFYCGRYLSWVVNETNLPDELNITAVDTMGNIMSVEHKS
                     LPCYGVQYHPESVMTPMGKQILKNWLML"
     gene            760..1143
                     /locus_tag="PLO59_10070"
     CDS             760..1143
                     /locus_tag="PLO59_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008240081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HQV01496.1"
                     /translation="MKKIINTENAPLPIGPYSQAVLCNNMLYVSGQIAINPATQQIET
                     NSIADETHQVMQNIKAILNCANLNFDAVVKTTIFLKSMQQFAAVNEVYATYFTGNFPA
                     RETVEVSCLPKNVNVEISITAYAPN"
BASE COUNT          382 a          213 c          208 g          412 t
ORIGIN      
        1 cctctgttct tttttatctt ttcaaataaa tcttgcatac attatatatt tataaatttt
       61 acgtgccaaa agtataaaaa taattgtgct gctattgatt actttacctc cataccaata
      121 cgtatttgtt ggtacgttac aacatcaacc agttttttaa tatttgtttt cccattggtg
      181 tcataactga ctcagggtga tattgtacac cataacaggg taatgattta tgttcaacac
      241 tcataatgtt gcccatagtg tcaacggctg taatgttgag ttcatccggc aagtttgttt
      301 cgttgactac ccatgagaga taacgcccgc aataaaaatc agcaggtata tctttgtaca
      361 atgcatctgg ttgcattaca tgtgttagcc gggatacacc atgttgtacg gtttgcaaat
      421 tttttagtgt gcctccaaaa tgttgaacta tggcctgcat gcctaagcaa acgcctaata
      481 ttttttttgt tgctacatac tgtttaatta cttccatcat taagccggct tcgttgggta
      541 agccgggccc gggtgatagt acgatggact gataaatttc aatttcgttt aaatatattt
      601 catcgttacg tataacatca atttgaaagc ttgttagctg ctgcaaatag tgatataggt
      661 tgtaagtaaa cgagtcgtaa ttaaccaata gaagaatttt caaaatcagc ggtttataat
      721 ttactttgac tgttacttta aaattacaat tgttctcaaa tgaaaaaaat tattaatacc
      781 gaaaatgcac cacttccaat tgggccatat agtcaggctg tactttgtaa taacatgttg
      841 tatgtatcgg gtcaaattgc aattaatccg gcaacgcaac aaatagaaac caatagtatt
      901 gctgacgaaa cacatcaggt aatgcaaaat attaaagcca tattaaactg tgcaaattta
      961 aatttcgatg ctgtagttaa aactaccata tttttaaaaa gcatgcaaca atttgctgct
     1021 gtaaacgaag tgtatgcaac ctattttaca ggaaattttc cggcacgcga aactgttgaa
     1081 gtatcgtgtt tgcctaaaaa tgtaaatgtt gaaataagca ttacagcgta tgcacctaat
     1141 taaatattaa gccaattttt acgaaccaaa tcttgttcgg ctgttgtttt ttgcatgcgc
     1201 tcaaccttgt agagg
//
LOCUS       DAOMAA010000833         1214 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_149126_length_1214_cov_3.690250, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000833 DAOMAA010000000
VERSION     DAOMAA010000833.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149126_length_1214_cov_3.690250"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..>1214
                     /locus_tag="PLO59_10075"
     CDS             101..>1214
                     /locus_tag="PLO59_10075"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HQV01497.1"
                     /translation="MKNPILFIFITVLIDCVGIGIIFPVAASIVTEVSGLTVNEATTY
                     SGYMMACYALMQFICAPTLGGLSDSYGRRPILLISLLGLGIDYLFLAVANSLPLLFLG
                     RILAGICGASFTTAFAYIADVSKPEKRAQNFGLVGAAFGLGFIIGPLIGGIASQWGVR
                     VPFIVAACFSLLNFFYGYFILPESLQPVNRRKFDFKRANPLGAFAQLRQNKNIRTLVF
                     GLFLIYLTGQVMPAIWTFYTKYVYNWSDTQIGYSLAYVGVMVVIVQGFLIKKVQKILG
                     SVNTIYFGLICYFIGLVLFAFATKPWMLYVFTTIYCLGGVAPPSLQGLLSNRVQANEQ
                     GELQGMMTALTSLSTILSPLIMTQLFYTFTKPDAIIH"
BASE COUNT          325 a          217 c          241 g          431 t
ORIGIN      
        1 ataaatttgt tcacgctact caaatacaaa caacatataa aaaatacttt gtcgaagtta
       61 acctgtttaa agtttaataa tcatttataa aaacttcctg ttgaaaaacc ccattctgtt
      121 tatattcatt acggtattga ttgattgcgt tggcattggt attatttttc cggtggcagc
      181 cagcattgta accgaagtta gcggattgac tgttaacgaa gccacaacct atagtggata
      241 tatgatggcc tgttatgcat taatgcaatt tatttgtgct ccaactttag gtggcttaag
      301 cgatagttat ggccgcagac ctattttact tatttccttg ttgggattag gtattgatta
      361 cttgttttta gcggttgcca atagcctgcc actactcttt ttagggcgca ttttagccgg
      421 tatttgtggt gccagtttta ctacagcttt tgcatatata gccgatgtaa gtaagcccga
      481 aaaacgtgca caaaatttcg gtttggttgg tgctgctttt ggtttgggat ttattattgg
      541 cccattaatt ggcggcattg ccagccaatg gggtgtgcgt gttccgttta ttgttgcagc
      601 gtgcttttcg ctgctaaact ttttttatgg ctactttatt ttacccgaat cgttacaacc
      661 tgtaaaccgc agaaagttcg attttaaacg tgctaatccg ttgggtgcat ttgcacaact
      721 acggcaaaac aaaaacattc gtacactggt gtttggcttg tttttaattt acttaacagg
      781 ccaggttatg ccagctatct ggacatttta taccaagtac gtatataact ggagtgatac
      841 acaaattggc tattcattag catacgtggg tgtgatggta gtaattgtac aaggcttttt
      901 aattaaaaaa gttcaaaaaa tattgggtag tgttaacacc atttactttg ggcttatatg
      961 ttattttatt ggcttggtat tgtttgcatt tgctaccaag ccttggatgt tgtatgtttt
     1021 tacaaccatt tattgtttgg gtggtgtagc acccccatca ttgcaaggat tgttatcaaa
     1081 ccgtgtacaa gctaatgaac agggcgaatt acaaggcatg atgactgcat taaccagttt
     1141 atcaaccata ctatcgccac taataatgac gcaacttttt tacactttta caaagcccga
     1201 tgctattatc catt
//
LOCUS       DAOMAA010000834         1214 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_149196_length_1214_cov_2.614323, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000834 DAOMAA010000000
VERSION     DAOMAA010000834.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149196_length_1214_cov_2.614323"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1214)
                     /locus_tag="PLO59_10080"
     CDS             complement(<1..>1214)
                     /locus_tag="PLO59_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01498.1"
                     /translation="APLQVSLGADISQCANLGNPVLNTQLVGVTTSWLYNGNNLPDTA
                     SFISATQTGQYIVNVSASPTCNGSDTVNVNLIAAPTATILANGISTDQVTICVGTPFP
                     TLDGTVAGAVEYVWTLNGTVVSNAATFVPTQDGSYTLQTSSLPNGGCATTDLITITTV
                     QSIVVNLGNNITQCANVAPPVLDAGVFPGANYLWSNGETTQTVTATVAGTYIVTVTYG
                     VICSASDTIDVIFDAVPAIPALADQNICYNIAISPLNANPFGTQPPTATYTWTDAGGA
                     VVGNAALFNPLVAGTYTVSISNNGCSVTDDATVTVEAQIPLNLGAPQTICGAPILIDA
                     GSAGQVTAYNWTKDGSPFGNTQTINANGPGTYTVTTTTALGCTSVASVQINAGTFATT
                     ITGDNVICENSS"
BASE COUNT          340 a          245 c          263 g          366 t
ORIGIN      
        1 cgctactgtt ttcgcaaatt acattgtcgc cagtaattgt tgttgcaaaa gtacctgcat
       61 taatttgaac agatgcaaca gatgtacatc ccaatgctgt agtcgtggtt acagtgtaag
      121 taccggggcc atttgcattt atagtctgtg tgttgccaaa tgggcttcca tcttttgtcc
      181 agttatatgc agttacctga ccggcagagc ctgcatcaat taaaataggt gcaccacata
      241 tagtttgtgg agctcccaag tttaatggaa tttgagcttc tacagttacg gtagcatcat
      301 cggtaacaga acatccattg tttgaaattg aaactgtata tgtacctgct accaacggat
      361 taaacaatgc ggcattgcca actactgcgc cacctgcatc agtccaggta taagttgctg
      421 taggtggttg tgtaccaaac ggattggcat ttagtgggct tattgctatg ttataacaaa
      481 tattttgatc ggccaatgcc ggtattgcag gcacggcatc aaaaattaca tcaatcgtat
      541 cgcttgcact acagataacg ccataagtaa ctgttactat ataggtacca gctacagttg
      601 cagttacggt ttgggtagtt tcgccattgc tccataaata attagcaccc gggaaaacac
      661 ctgcatctaa aaccggtggc gctacgttgg cgcattgtgt aatattgtta cctaaattta
      721 ctactattga ttgaacagta gtgatggtta ttaaatcggt agtggcgcag ccgccattag
      781 gtagtgagct tgtttgtaaa gtatagctac catcttgggt aggtacaaag gttgctgcat
      841 tgctaactac ggtaccattt aatgtccaca catattctac tgctcctgct accgttccat
      901 ctaatgttgg gaaaggtgta cccacacaaa tggtaacctg atcggttgaa ataccatttg
      961 ctaaaatggt ggctgtaggc gctgctatca gatttacatt tactgtatca gaaccattac
     1021 aagtagggct tgccgaaaca tttacaatgt attggccggt ttgggtggca ctaataaaac
     1081 tggctgtatc aggtaaatta ttgccattat ataaccaaga tgttgttaca ccaactaatt
     1141 gtgtgtttaa taccgggttg cctaaattgg cacattgcga aatatcagca cctaaagaca
     1201 cctgaagtgg agct
//
LOCUS       DAOMAA010000835         1211 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_149923_length_1211_cov_2.931661, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000835 DAOMAA010000000
VERSION     DAOMAA010000835.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149923_length_1211_cov_2.931661"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1211
                     /locus_tag="PLO59_10085"
     CDS             <1..>1211
                     /locus_tag="PLO59_10085"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HQV01499.1"
                     /translation="PATNVGAFRAQSLQSYNGKVLRIDPMTGYGVPSNPYYNAANPDG
                     VESKIWALGMRQPFSLTIRPGSGSTNASSGNPGAIYVNDVGFYNWEEENVVTAPGQNF
                     GWPIYEGFLKHPNFSSANVYNLNAPNPLYGTGGCTTPYFKFADLLKDDTQGPGSWPNP
                     CNTSVQIPANIPVFKHRRGSYAYKHGTPIVYVPEYTGNNPLAANLGSPQTSATGTPFD
                     GSCIMGSAWIPNNAWPAKYHDAMFFMDYDKGWIRYAKFDANHKLVSIDTFATNVGRPV
                     TMQWHPIQKCIYWVDYISGQLHRIKYTVGANLAPVAIATQNKVYGASPVNVQFDGTAS
                     YDPETAPLTYFWSFGNGVTSTLANPSITFTAPTSGNIILIIWTSCGAAEIQTDNGIPT
                     FACTGNGTVVQ"
BASE COUNT          360 a          250 c          262 g          339 t
ORIGIN      
        1 cctgcaacca atgtgggcgc atttagagca caaagtctgc aatcgtacaa tggcaaagta
       61 ttacgcattg atccaatgac gggttatggc gttccgtcaa acccatatta caatgcagca
      121 aatcctgatg gtgttgaaag taaaatttgg gcattaggca tgcgtcaacc tttttcactc
      181 accatccgcc cgggtagtgg tagtaccaat gcttcgagcg gcaaccccgg tgctatttat
      241 gtaaatgatg ttgggtttta taattgggaa gaagaaaatg tggttactgc accgggccaa
      301 aattttggct ggcctattta tgagggattt ttaaaacacc ccaattttag ttcggcaaac
      361 gtttataact taaatgcgcc caatccactt tatggcacgg ggggatgcac tacaccctat
      421 tttaaatttg ccgatttatt aaaagatgac acacaaggcc cgggttcatg gccaaatcca
      481 tgcaatacaa gtgtgcaaat tccggccaac atacctgtgt ttaaacatcg cagagggtcg
      541 tacgcatata aacacggcac ccccatagtt tatgtgccag aatataccgg aaacaatcca
      601 ttggctgcaa atttaggaag tccgcaaacc tctgctacag gaacgccctt cgatggttca
      661 tgtattatgg gtagtgcatg gataccaaat aatgcctggc ctgctaaata tcacgatgct
      721 atgtttttta tggattacga taaaggttgg atacgctatg caaagtttga cgcaaatcat
      781 aaattggttt ccattgatac gtttgcaacc aatgtaggca ggcctgttac aatgcaatgg
      841 catcccattc aaaaatgtat ttattgggtt gattacatta gtgggcaact acaccgaata
      901 aaatatacag taggcgcaaa tttggcaccg gttgcaattg caacacaaaa taaagtatat
      961 ggtgcatcac cagttaatgt tcaatttgac ggtacagcaa gttatgatcc cgaaacagca
     1021 ccgcttacct atttttggag ctttggtaat ggtgtaacca gcacgctggc aaacccatca
     1081 attactttta cagcaccaac tagtggaaat atcatattga ttatttggac gagttgtgga
     1141 gctgctgaaa tacagactga taatggtatt ccgacttttg catgtactgg caatggtact
     1201 gtggttcaat g
//
LOCUS       DAOMAA010000836         1211 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_149924_length_1211_cov_2.923875, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000836 DAOMAA010000000
VERSION     DAOMAA010000836.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149924_length_1211_cov_2.923875"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="PLO59_10090"
     CDS             complement(<1..420)
                     /locus_tag="PLO59_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HQV01500.1"
                     /translation="MVFSSSLFLVYFLPLFLLVYYAAARPYKNGVLLVFSVLFYAWGA
                     PKFIFVLLGTTLLDFFLVQWMDRTTNQRWRKIALCISVGINISLLFYFKYCNFFIETI
                     NDVLHTAGIGPIKLLQVVMPIGISFYTFETMTYVVDVY"
     gene            599..>1211
                     /locus_tag="PLO59_10095"
     CDS             599..>1211
                     /locus_tag="PLO59_10095"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HQV01501.1"
                     /translation="MKALLFIALIIATNSWAQTKHLTIEEAVSKQRTTLGPKRLKHLQ
                     WIKDTDQFIYAAADGAERFVIGNADSKKNEDWLTLLALNEKLREQQLDTLKTLPALKW
                     LNSNQATFTVADNDVVITKSNLALHTVQNPKLPTDAANMDVATNKNTAYTLANNLYIN
                     IGYEKVAVTADSNKHIVNGQSVHRNEFGIHKGTFWSSTGRYLAY"
BASE COUNT          460 a          226 c          225 g          300 t
ORIGIN      
        1 gtaaacatca accacataag tcatggtttc gaaagtgtaa aacgatatac caataggcat
       61 tactacctgc aacaatttta tgggccctat gcctgctgta tgcaaaacat catttatggt
      121 ttcgataaaa aagttgcagt acttaaaata aaagagcagg cttatattaa tgcccacact
      181 tatgcatagt gcaattttac gccatcgttg gttggtggta cggtccatcc attgtaccag
      241 aaaaaaatcg agcagggtag tgcccaacaa cacaaaaata aatttgggtg caccccatgc
      301 ataaaacaaa acactaaaca ccagcaacac gccattttta tagggccgcg ctgctgcgta
      361 atacaccaat aaaaaaagtg gtaaaaagta aactaaaaat aaactgctgc taaaaaccat
      421 tcaggtataa gcctttaata aggcgcgcaa agaagataaa atttatgcaa atgttaagta
      481 ttgtcagcta tggcttctca aaaaaatgta atacttacgc aggcttaatt taaagttgtt
      541 tagggcagta aaagcctgac aaaagcggaa aaacgaataa gcataaaaaa caaaaagcat
      601 gaaagcactt ttatttattg cattaataat tgccactaac agttgggcgc aaaccaaaca
      661 tttaaccata gaagaagccg tatcgaaaca acgtaccacc ttagggccta aaaggcttaa
      721 acatttacaa tggataaaag ataccgatca gtttatttat gcagcagccg atggtgccga
      781 acgttttgtt attggtaatg ccgatagtaa aaagaacgag gactggctaa ccttgttagc
      841 gcttaatgaa aaacttagag agcaacagtt agatacatta aaaacattgc cggcattaaa
      901 gtggcttaac agtaaccaag ctacttttac ggtggctgat aacgatgtag taattaccaa
      961 aagtaatttg gcattgcata cggtacaaaa cccaaaactg ccaaccgatg ctgctaacat
     1021 ggatgtggca accaataaaa atacagcata tactttagcc aataatttgt atataaatat
     1081 agggtatgaa aaagtagccg ttacagccga tagcaataaa catattgtaa acggacaaag
     1141 cgtacaccgt aacgaatttg gcatacacaa aggcactttt tggagtagta ccggcaggta
     1201 tttagcatac t
//
LOCUS       DAOMAA010000837         1210 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_150121_length_1210_cov_4.185281, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000837 DAOMAA010000000
VERSION     DAOMAA010000837.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150121_length_1210_cov_4.185281"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="PLO59_10100"
     CDS             <1..403
                     /locus_tag="PLO59_10100"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HQV01502.1"
                     /translation="WTFGDGSTASIASPVHTYINTCSYQATLTAMHCSTSHSITHTVN
                     VSSVAAIPNINQQQLINCYPNPADKFMWLKLNKHLLDQPYCITDLNGKTLLMGMAIDL
                     NIKIDIEHLTEGIYILKMGNNAPQKFSIIRR"
     gene            840..>1210
                     /locus_tag="PLO59_10105"
     CDS             840..>1210
                     /locus_tag="PLO59_10105"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HQV01503.1"
                     /translation="MSSKLIKRKSNHNRFINLTVLCLTFLFKAYAQLPPHFYDLPVVS
                     GMLKVEGVVFDQNHRMYAWEKTGKVWIIDSNGIMNTNPLLSITSEVGSWVDHGMNGFA
                     LDPDFANNGYFYVYYTVDRHHL"
BASE COUNT          422 a          218 c          192 g          378 t
ORIGIN      
        1 gtggactttt ggcgatggta gcaccgcttc tattgcatca cctgtacata cttacataaa
       61 cacatgtagt tatcaggcaa cgttaactgc aatgcattgc agcacatcac attcaataac
      121 gcatacggta aacgtaagtt cagttgcagc tatacccaat attaatcaac aacaattaat
      181 aaactgttac cctaacccgg ctgataaatt catgtggtta aaattaaaca agcatctgct
      241 tgatcaacca tactgtatta ccgatttaaa tggtaaaaca ttgcttatgg gtatggctat
      301 tgatttaaat atcaaaatag atattgagca cctaactgag ggaatttaca ttttaaaaat
      361 gggtaacaac gcaccacaaa agtttagtat tatcagacga tagcatattt tggtcttcat
      421 ttaattgtaa ctggtaaaaa aaattgaaga taaatagcat cagaattaaa gtcttcctgt
      481 attaaaatgg cgctaacttg taagcgttat actttttcat tttacttctt agttctttat
      541 tgccttggtt gcttaaaaac agcacaatat gtatcaacta tttaatgggt ttaataacac
      601 gcaacaatac atgtttatgg ctaaaatttg cacatcagtg tttaaacaag ttcttctcta
      661 caaaagaaaa acaaattaaa gatacatatg gtgtaacaac tcagtttcga catatcaaca
      721 tgagtaaaat cataaaagtt atgatattga attttgtcat tgcacaaaaa atcagataag
      781 caaaattaaa agtctgttta cctgatttga taataccaaa ccatttaatt cgcagccaca
      841 tgagctctaa acttattaaa cgcaaaagta accacaaccg atttattaac cttactgtac
      901 tttgcctaac ctttttattt aaagcctacg cacaattacc accacatttt tatgatctcc
      961 ctgtagtgtc aggcatgtta aaagttgagg gtgttgtgtt tgatcaaaat caccgcatgt
     1021 atgcctggga aaaaacgggc aaagtttgga taatagattc aaacggcata atgaatacca
     1081 acccgttatt atcaataacc agcgaagtag gaagttgggt tgatcatggg atgaatggct
     1141 ttgcacttga tcctgacttt gcaaacaatg gctactttta tgtatattat acggttgacc
     1201 gccaccatct
//
LOCUS       DAOMAA010000838         1210 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_150139_length_1210_cov_3.432900, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000838 DAOMAA010000000
VERSION     DAOMAA010000838.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150139_length_1210_cov_3.432900"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1210
                     /locus_tag="PLO59_10110"
     CDS             <1..>1210
                     /locus_tag="PLO59_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HQV01504.1"
                     /translation="LNQFGVNYEFISEEMDLYHGNPKAESPQNSEDDNMAREDEPASY
                     SQSKKPGDSKSKTPVLDNFGRDLTKAAEEGRLDAIVGREKEIERVSQILSRRKKNNPI
                     LIGEPGVGKSAIAEGLALRIMQRKVSRVLFGKRIVTLDLASLVAGTKYRGQFEERMKA
                     VMNELEKSPDVILFIDEIHTIIGAGGASGSLDASNMFKPALARGEIQCIGATTLDEYR
                     QYIEKDGALDRRFQKVLVDPTSMDETIEILNNIKDKYEEHHHVNYTPEAIKACVKLTS
                     RYITDRFLPDKAIDALDESGSRVHLKNIHVPENIVDLEQKIQDVKAEKNRVVRNQKYE
                     EAARLRDKEKTLIEQLDLAKRLWEEETKNQRFTVTDENVAEVVAMMSGVPVTRIAQAE
                     TARLVSMPDEL"
BASE COUNT          413 a          221 c          276 g          300 t
ORIGIN      
        1 ttaaatcaat ttggagttaa ctacgaattt attagcgaag aaatggatct gtaccatggc
       61 aaccctaaag cagaatcgcc acaaaacagc gaagatgata acatggcccg tgaagacgag
      121 cctgcttctt acagccaaag caaaaaaccg ggtgatagca aatcgaaaac accggtgctt
      181 gataactttg gtcgcgattt aaccaaagct gctgaagaag gtagattaga tgccattgta
      241 ggtcgcgaaa aagaaatcga acgtgtatca caaattttat cgcgcagaaa gaagaacaac
      301 cccattttaa ttggcgaacc cggtgttggc aaatcggcta ttgccgaagg tttggcattg
      361 cgtattatgc aacgtaaagt atcgcgtgtg ttattcggaa aaagaattgt tacgcttgat
      421 ttagcaagct tagttgccgg tactaaatat cgcgggcaat ttgaagagcg tatgaaagcc
      481 gtgatgaatg agttagaaaa atcgcctgat gtaattttgt ttattgatga aatacacacc
      541 ataattggtg ccggaggtgc ttcgggctca ttagatgcca gcaatatgtt taaaccggct
      601 ttggcacgtg gcgaaattca atgcattggc gcaactacat tagacgaata tcgtcagtat
      661 attgaaaaag atggcgcttt agacagacgc tttcaaaaag tattggttga ccctaccagt
      721 atggacgaaa ccattgaaat acttaataac attaaagata agtacgagga gcatcaccat
      781 gtaaattaca cacctgaagc tattaaagca tgcgttaaac ttacatcgcg ttatataacc
      841 gataggtttt tacccgataa agctattgat gctttagacg agtcgggctc gcgtgtacat
      901 ttaaaaaata tacatgtgcc tgaaaacatt gttgatctgg aacaaaaaat acaagacgtt
      961 aaagccgaaa aaaaccgggt agtgcgtaac caaaaatatg aagaagcagc acgcttgcgc
     1021 gataaagaaa aaacgctgat tgagcaattg gatttggcga aacgtttatg ggaggaagaa
     1081 accaaaaacc aacgctttac tgtaaccgat gaaaatgtgg ccgaagtagt tgccatgatg
     1141 agtggtgtac cggtaacacg cattgcacaa gccgaaactg cacgtttggt aagtatgcct
     1201 gatgaactta
//
LOCUS       DAOMAA010000839         1210 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_150150_length_1210_cov_3.088312, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000839 DAOMAA010000000
VERSION     DAOMAA010000839.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150150_length_1210_cov_3.088312"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /gene="cdaA"
                     /locus_tag="PLO59_10115"
     CDS             <1..799
                     /gene="cdaA"
                     /locus_tag="PLO59_10115"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HQV01505.1"
                     /translation="RNQKYMFDLFRVDFIHFRLLDLIDILLVTYIFYRVFLIIRDSVA
                     VKILLGVLLIYLLWWLFARVLHMTLIGTLMGIFINVGIIGLIIVFQPEIRRFLVLIGT
                     NSFFTKRFFKSGLFGFDSVQASPLNATPILDACQLLSKSNTGALIVISRATSLNYIIE
                     TGQKIAAEISTPLIESIFLRTAPMHDGALIIENNQIAAARCLLPVSANDNLPVHLGTR
                     HRAAIGITENTDAIALVVSEETGSISLAIDGELHSNLKIEAIEAFLK"
     gene            complement(857..1117)
                     /locus_tag="PLO59_10120"
     CDS             complement(857..1117)
                     /locus_tag="PLO59_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013597505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HQV01506.1"
                     /translation="MKKDIHPKNYRLVVFKDISCDYAFLTRSAVETKDTIKWEDGNEY
                     PLVKLELSHMSHPFYTGKMKLVDTAGRVDKFRTRYAKKTANA"
BASE COUNT          367 a          194 c          221 g          428 t
ORIGIN      
        1 tcgaaaccag aaatatatgt tcgacttatt tagggttgat tttattcatt tcagactatt
       61 agatttaata gatattttac ttgttaccta tatattttat cgtgtttttt taataatacg
      121 ggatagcgtt gcagtaaaaa tattattagg cgttttactg atttatttac tatggtggct
      181 ttttgcacgt gtactgcaca tgacacttat tggtacctta atgggtattt ttataaacgt
      241 tggtattatt gggttaatta ttgtgtttca gcccgaaatc aggcggttct tggttcttat
      301 tggtaccaac agttttttta ccaaacgctt ttttaaatca gggttgtttg gttttgatag
      361 cgtacaagcc agcccattaa atgcaacacc tattttagat gcatgccaat tgcttagtaa
      421 atcaaataca ggggcactta ttgttataag cagggctacc agcttgaatt atattattga
      481 aaccgggcaa aaaatagctg ctgaaatatc aactccttta atagaaagta tttttttaag
      541 aacagcaccg atgcatgatg gagctcttat tattgaaaac aatcaaattg cagcagcgcg
      601 ttgtttgtta cctgtttcgg ctaacgataa cctgcctgta catttgggta cacgtcatag
      661 agcagccatt gggataactg aaaacaccga tgctattgcc ttggtagtaa gtgaagaaac
      721 aggaagtatt tcattagcaa ttgacggtga gttgcactca aatttaaaaa tagaggcaat
      781 agaagcgttt ttaaagtgat ggttaagttt ctaaatggtg caaaaaaaaa ccttgcattt
      841 tgcaaggttt ttaaatttat gcgttagctg tctttttagc ataacgtgtg cggaacttat
      901 caacgcggcc tgctgtatca accaacttca tcttaccggt ataaaaagga tgactcatgt
      961 gcgaaagttc taattttacc aatggatact catttccatc ttcccatttt attgtgtctt
     1021 tggtttcaac tgccgaacgg gttaaaaacg cataatcaca tgatatatct ttgaaaacaa
     1081 ctaaacggta gttctttgga tgaatgtctt ttttcatttc tttaaaaatt tgaggcggca
     1141 aatgtaattt ttttaggtat taaaacaaac cactaagtca ttttaatcaa acttctattg
     1201 ttaataaacc
//
LOCUS       DAOMAA010000840         1209 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_150392_length_1209_cov_2.925477, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000840 DAOMAA010000000
VERSION     DAOMAA010000840.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150392_length_1209_cov_2.925477"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="PLO59_10125"
     CDS             complement(<1..446)
                     /locus_tag="PLO59_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019388626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQV01507.1"
                     /translation="MKLLSGKTALVTGASRGIGRGIAIKLAQQGANVIFTYSASAQKA
                     EELATLLQQEGVKALAVKSDAADYNAAEALIATILAEFTTIDIVINNAGITRDNLLMR
                     MTEAHFDEVIATNLKSVFNITKAVQKQLLKQRSGSIINISSVVGVKG"
     gene            563..>1209
                     /locus_tag="PLO59_10130"
     CDS             563..>1209
                     /locus_tag="PLO59_10130"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HQV01508.1"
                     /translation="MKKITLIIAFAGITLQALSQGLEVGLNGSLGTTWLLNKNVFDQD
                     EILNPTFSGGFTFGASATYFFSPKLGVGIELNSATVNQKYSGEYSANDTYEAKTTLKY
                     LSIPVLLKLQTESGFYFEIGPQFNSLSKAKEEYTSDPSDPDDYSSLNVKKGFENMVIA
                     GVFGFGGRFDLNDNLALTAGVRFQYGIGDVTKEYSQTELLDLVTTGEGIYGEAGLT"
BASE COUNT          362 a          218 c          232 g          397 t
ORIGIN      
        1 ccttttacac ctaccacact actaatgtta ataattgaac ccgagcgctg ttttaataat
       61 tgtttttgca cggctttggt aatattaaaa accgatttta aattggtggc tatcacttca
      121 tcaaaatgtg cttcagtcat acgcatcaat aaattatcgc gtgttatacc ggcattgttt
      181 atcacaatat caatggttgt aaactcggct aatatggtag ctattaacgc ttcggctgca
      241 ttataatcgg ctgcatcact tttaactgcc aacgctttta caccttcttg ttgtaagagc
      301 gttgccaact cttcggcttt ttgtgctgat gcactgtaag taaaaataac attagcacct
      361 tgttgtgcta acttaatggc aatgccacgg ccaataccac gacttgcacc ggttactaaa
      421 gctgtttttc cgctaagtaa tttcataagg tttgtattaa atttttggga agtatgaaaa
      481 ttttatccaa aacaacaaac agtttgtgct ttaaggttta aatctatttt tgcatgcatt
      541 aatttaaaac tctcaaacaa aaatgaaaaa aattaccctg attattgcct ttgcaggcat
      601 aaccttgcaa gcactttcgc aaggcttaga agtgggctta aatggaagtt taggtactac
      661 ctggttactt aataaaaatg tttttgacca agacgaaatt ttgaatccaa cctttagcgg
      721 tggctttact tttggagctt ctgcaactta ttttttttcg cctaaattgg gcgtaggcat
      781 cgagttaaat tcggccacgg ttaatcagaa atatagtggc gagtatagtg ctaacgacac
      841 gtatgaggcc aaaacaactt taaagtattt atccattccg gtattattaa aactgcaaac
      901 cgaaagtggt ttttactttg aaattggacc acagtttaat tcattaagca aggccaaaga
      961 agaatataca agcgatccat ctgatccgga tgattacagt tcgttaaatg tgaaaaaagg
     1021 atttgagaac atggttattg ccggtgtttt tggctttggc ggccgttttg atttaaatga
     1081 taatctggcg cttacagccg gtgtgcgttt tcagtacggt attggcgatg ttaccaaaga
     1141 atatagtcaa acagaattgc ttgatttagt tacaacaggc gaaggtattt atggcgaagc
     1201 tggcttaac
//
LOCUS       DAOMAA010000841         1207 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_150881_length_1207_cov_2.603299, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000841 DAOMAA010000000
VERSION     DAOMAA010000841.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150881_length_1207_cov_2.603299"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1073)
                     /locus_tag="PLO59_10135"
                     /pseudo
     CDS             complement(1..1073)
                     /locus_tag="PLO59_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012781016.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
BASE COUNT          341 a          253 c          225 g          388 t
ORIGIN      
        1 gtaagtggat gatagatatc atttagcgat acgcggccta aaattctgtc atataacgat
       61 tcaacaattt cctcattatt tttcaatgca gtttcggcca aaccacgtag ggtaccacaa
      121 tcaacttcgg taataattac atcttgtgcc acgtcaacca aacgtctggt taaataaccg
      181 gcatcggctg ttttaagagc cgtatcggcc aaacctttac gagcaccgtg ggtagagata
      241 aaatattcta agatggataa accttcttta aagtttgaaa gaattggatt ttcgataatc
      301 tcgcctccgg ttgagcctgc tttttgcggc ttagccatca aaccgcgcat accgcttaac
      361 tggcgaattt gatctttact accacgggcg ccactatcca acatcatata tactgagttg
      421 aagccttgct tttcagtgct gatggttttc atcagatttt cggtaatgcg cgagttggta
      481 cgtgtccaaa tatcaattat ctggttgtaa cgctcgttat ctgtaataaa tccgttttga
      541 taattatcca atactgaatt tacttcgtca tcggcttttg aaatcagttg atctttattt
      601 acatcaatat caatatcact taagttgaat gataagcctc ctttaaaggc cattctaaat
      661 cctaagtctt taatatcatc taagaatttt gctgtaccgg ctatgccgca tgcttttaaa
      721 acgcgggcaa taatatcgcg taacgatttt ttggtaagta actcatcaat atatccaact
      781 tcgcgtggta caacctgatt aaatattaca cggcctacgg tactttcgat aactttatgg
      841 ttaagggtat cgttttcgcg aacgtaagca cgtactttaa tgtgtgcatg caaatcaaca
      901 cggccttcgt tataggcgat taaaacttcc tcatcgctat aaaacaccaa accttcgcct
      961 tttactttgg tttcggcagt gctgcgttta cctttagtta tatagtacaa gcctaatacc
     1021 atgtcttgcg agggtacggt aatgggcgct ccgttggccg gatttaatat gtttcagtcc
     1081 tttgaaattg gttttgtgaa aagcattgga gaaacaattg gctcaaccat ttcttctgta
     1141 atttcaaata cactgactaa acgtcttctt gaagaaacac gtcaacaagc tgaacgatgg
     1201 aagatgc
//
LOCUS       DAOMAA010000842         1205 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_151352_length_1205_cov_2.616522, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000842 DAOMAA010000000
VERSION     DAOMAA010000842.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151352_length_1205_cov_2.616522"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PLO59_10140"
     CDS             complement(<1..242)
                     /locus_tag="PLO59_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HQV01509.1"
                     /translation="MKPEIQLAVFASGNGSNAQNLITFFEGSRIKIALILSENPNARV
                     LQRATLFQVPSVIFSMSELQNGQVLQQLKGYGINYIV"
     gene            504..>1205
                     /locus_tag="PLO59_10145"
     CDS             504..>1205
                     /locus_tag="PLO59_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020604690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HQV01510.1"
                     /translation="MNKIFTHHYFSKKIIFQVVLSVLAIVLSTISLKLQAQNYPSGFS
                     QIQLANGINAPTAMAFAPDNRIFICRKNGQILIFKNGALNATPFVSINTTPSGERGLL
                     GIALDPNFETNNYVYVYYTLASGARNRISRFTANGDVAVTGSELVILDLDPLSTATNH
                     NGGTIKFGLDGKLYVSVGDNYNSANAQNLNTYHGKLLRINADGSIPTNNPYTTGTGQK
                     KRIWATGLRNPFSFDI"
BASE COUNT          400 a          220 c          199 g          386 t
ORIGIN      
        1 acgatataat ttattccata gcctttaagc tgttgcaaaa cttgtccgtt ttgcaattcg
       61 gacatcgaaa atataacaga tggcacctga aataaagttg ccctttgtaa gactctggca
      121 ttcggatttt cacttaaaat gagggcaatt ttaatacgac tcccttcaaa aaaggtaatc
      181 agattttgtg cattactacc attaccactg gcaaaaacgg ctaattgaat ttcgggtttc
      241 atcaacgcaa aacaacaata aagtttgcaa ttgcaaggtg tagcttatgg ttaacaagtg
      301 tattgataac tacttcaaac aatttcaaaa tttatagggt atcaatacaa atgttgttat
      361 tttgccattg ttaataaatt gttcgtatct tgtggtaatt agcacaaacc ccatatttta
      421 ttatatcaaa attgttagta ttcgaatttt tattaacaca cagcgcttac ttaataatta
      481 ctgctcccaa ttttaaattc gctatgaata aaatatttac gcatcattat ttttcaaaaa
      541 aaattatttt tcaggttgtc ctaagtgtat tggctatcgt tttaagtact attagtttaa
      601 agctacaagc gcaaaactat cccagtggat ttagtcaaat acaattagcc aatggcataa
      661 atgcgcctac agcaatggca tttgcacccg acaaccgtat ttttatttgc cgtaaaaatg
      721 gacagatttt aatttttaaa aatggcgctc ttaatgcaac accttttgta tccataaata
      781 ctactccatc gggcgaacgt ggccttttag gcattgcctt agacccaaat tttgaaacaa
      841 ataattacgt gtatgtgtat tacacattag catcgggcgc gcgtaaccgt attagtcgtt
      901 ttacagcaaa tggcgatgtt gccgttacag gctcagagtt ggtaatactt gatttagacc
      961 cgttaagtac cgccaccaat cataatggag gtaccattaa atttggttta gatgggaaac
     1021 tatatgtgtc ggttggcgat aattacaatt cggctaatgc acaaaattta aatacatacc
     1081 acggtaaatt attgcgaatt aatgcagatg gttcaattcc aacaaacaac ccatatacaa
     1141 caggtacagg tcaaaaaaag cgaatatggg caaccggatt acgcaatcca ttttcattcg
     1201 atata
//
LOCUS       DAOMAA010000843         1203 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_151796_length_1203_cov_3.182927, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000843 DAOMAA010000000
VERSION     DAOMAA010000843.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151796_length_1203_cov_3.182927"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="PLO59_10150"
     CDS             complement(<1..712)
                     /locus_tag="PLO59_10150"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HQV01511.1"
                     /translation="MTKISMFSSVRTDLSSGIVVFLVALPLCLGISLASGAPFFSGII
                     SGIIGGLIIGYFSASHLSVSGPAAGLAALVLAAILNVGSFQLFLCAVVIAGVLQIAMG
                     LASLGGISNYFPSSVIKGMLTCIGILIIAKQIPHAFGYNENVKGDFSEFIMVDESDHI
                     LEDIMHLNVGVFLICLLSIAIILICDKPKVKAKLKGIPGALIAVTVAVLINELLHFTG
                     NSFAIDNTHLVNINEAESA"
     gene            complement(781..1089)
                     /locus_tag="PLO59_10155"
     CDS             complement(781..1089)
                     /locus_tag="PLO59_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01512.1"
                     /translation="MSQRKLIILFVFFSVVNLYGSVVATVNYCIARNANQIEHSLDTP
                     IPNLNLIEETIHDKICITQTESVLEPMCYFVDFHVYRFINKGFDHSKILLQPPKKQVA
                     "
BASE COUNT          438 a          220 c          183 g          362 t
ORIGIN      
        1 gtgcactttc ggcttcgttt atgtttacca aatgggtgtt atcaattgca aatgagtttc
       61 cagtaaaatg tagcagctca ttaattaaaa cagctaccgt aacggctatt aatgcgcccg
      121 gtatgccttt taatttggct ttaactttag gtttgtcgca aattaaaatt attgcaatag
      181 atagcaaaca aattaaaaac acacctacat tcagatgcat gatatcttct aatatatgat
      241 cgctttcatc taccatgata aactctgaaa aatcaccctt cacattttca ttataaccaa
      301 aagcatgggg tatttgtttg gcaataatca aaataccaat acatgtgagc atgcccttta
      361 taacactgct gggaaaataa tttgaaatac cgcctaaact ggccaagccc attgcaattt
      421 gtaaaactcc ggctataaca acagcacaaa ggaataattg aaaactgcct acatttagta
      481 tggctgctaa aactaatgct gctaaaccgg ctgccggtcc acttacgctt aaatgcgaag
      541 cactgaaata accaataatt aatccgccaa taatgccgga tataatacca ctaaaaaatg
      601 gagcgccact ggctaagcta atacctaaac ataaaggaag tgcaactaaa aacacaacta
      661 tgccagaaga caaatcggtt cgcaccgatg aaaacatcga aatttttgtc ataataaatt
      721 ataaataaat agtagcctta aacaggctaa ttgtttgaaa atagtaaata aaaaaaatgc
      781 ttaagccacc tgctttttag gtggttgcag taaaatttta ctgtgatcaa accctttatt
      841 tataaaccga tacacatgaa aatcaacaaa ataacacata ggctcaagta cactttcagt
      901 ttgagttatg catattttat catgtatggt ttcttcaatt aaatttaaat tcggaatggg
      961 cgtatccaat gaatgctcaa tttgatttgc attgcgggca atacaataat ttacggtagc
     1021 aactacactg ccgtaaaggt ttacaacaga aaaaaacaca aataaaataa tgagtttgcg
     1081 ttgcgacatt tttattttaa aacctatttt taaagagtaa ttaatatccg tttaagtggt
     1141 tgcgctactt aacaacattt ttacttctat tatatcggtc ggcaaaaaaa ttaatttatt
     1201 tta
//
LOCUS       DAOMAA010000844         1202 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_152138_length_1202_cov_2.157803, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000844 DAOMAA010000000
VERSION     DAOMAA010000844.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152138_length_1202_cov_2.157803"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            11..172
                     /locus_tag="PLO59_10160"
     CDS             11..172
                     /locus_tag="PLO59_10160"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HQV01513.1"
                     /translation="MKENKISIFSPIATALIGYKVNDEIEWIVPAGLTKIKIDKIIYQ
                     PEAAGDYDI"
     gene            complement(453..>1202)
                     /locus_tag="PLO59_10165"
     CDS             complement(453..>1202)
                     /locus_tag="PLO59_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HQV01514.1"
                     /translation="KSFNEINKEFVVYESSFSDIIGVVLFNFFVTTEIINLTAGLKFT
                     SDLIFILIITCLSTIASAVLLYKINTHVKHVPLLALILLVYAITKLLHLPGLIYILIL
                     GLVFSNASMFNKFKIFKKVRFDNFNAEVNSFTDLVIEATFLIRILFFTLFGFSLNLDE
                     LISTTELPYSIAFVVLIFLSRAVLLHLYKLPLVPVLFVAPRGLITILLFLSVPITSQL
                     PLINKTLLIQMVLLTAFVLIFGGLLNKKSNL"
BASE COUNT          463 a          168 c          199 g          372 t
ORIGIN      
        1 taaggctgaa ataaaagaaa ataaaatttc aattttttcg cccattgcaa cggcattaat
       61 cggttacaag gttaatgatg aaattgagtg gatagtaccg gcaggattaa caaaaattaa
      121 aattgataaa ataatttatc agccggaagc cgcaggcgat tacgatatat aaacacagac
      181 attttaaatt ttttgtagta gtaatgactg tgagtttggg ataaatcaaa cggcaattat
      241 tactgttttt acattaacga agttgtatgg gtacagtgat taggcgtaat gcgtttttat
      301 taagacacgg taacgagtta ttataagtga ttaatcaatc aactataaat ttagttgcat
      361 taatcaagta atattccgta taaaatatgt atcgtttgtt tgcacacatc atttgcacgc
      421 tacattttat acagccttta atgcagcact tactaaaggt ttgatttttt atttagtaag
      481 cctccgaaaa ttaaaacaaa agctgttaac aaaaccattt gaattagcag cgttttattt
      541 attaaaggca attgactggt tattggtaca gaaaggaaaa gtaaaatagt gattaagcca
      601 cgcggagcta caaataaaac cggtaccaaa ggtagtttgt aaagatgaag taaaacagcc
      661 cgtgataaaa atataagtac aacaaatgcg atagaatagg gcaattcagt tgtacttatc
      721 aattcatcta aatttaaact aaaaccaaat agcgtaaaaa ataaaatgcg tattaaaaag
      781 gtggcttcaa taactaagtc ggtaaacgaa tttacttcgg cattaaaatt atcgaaacgt
      841 acttttttaa atattttaaa tttattaaac atgcttgcat tgctaaaaac caaacctaaa
      901 atcagtatgt aaattaaacc cggcaaatgc aacaatttgg ttatagcata tacaagtaaa
      961 attaatgcga gcaaaggtac atgcttaaca tgcgtattaa ttttataaag caaaactgcc
     1021 gatgctatgg tgcttaggca tgtaattatc aggataaaaa ttaggtcaga tgtaaatttt
     1081 aatccggctg ttaaatttat aatttcagta gtaacaaaga agttgaatag gacaacgcca
     1141 ataatgtcgc taaaactact ttcgtaaacc acaaactctt tatttatttc gttaaacgat
     1201 tt
//
LOCUS       DAOMAA010000845         1201 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_152403_length_1201_cov_2.157068, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000845 DAOMAA010000000
VERSION     DAOMAA010000845.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152403_length_1201_cov_2.157068"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1201
                     /locus_tag="PLO59_10170"
     CDS             <1..>1201
                     /locus_tag="PLO59_10170"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HQV01515.1"
                     /translation="PNNVIYGLLSDNAGNLWMSTNKGLCCLPASRTPNNANNKFSNTH
                     IESNPFICFNSDDGLPGDEFNRHEYFKLPNGDLYFEGVMGGVHFSPLKILEQEPNAPI
                     VLTKLLVYNKPVTFQTDSSIIQMPVSYAKSITLPPDKNMFTITFAALNYSSASKRLYK
                     YMLDGYDKQWIESGLKNEATYTNLAPGTYTFKVIHAQNNNSPQNETAIQIIIKPAWYQ
                     TWLFKITLTTLLAVSVIGLYRYRLNQQINLMHIRNRIASDLHDEIGSTLSSISISSTI
                     IQNKMNGHDSDVKKLLQQISINTDNMMEAMSDIVWSINTKNDRFDNIINRMRAFAIEM
                     MEPKNCMVHFNTKNINVNTKLDLLQRKNLYLIFKEAINNAAKYAQCKNVWIAITMQGN
                     KLIMCIKD"
BASE COUNT          444 a          229 c          199 g          329 t
ORIGIN      
        1 cccaataatg taatttatgg tttgttaagc gataatgccg gtaatttatg gatgagtacc
       61 aacaaaggtt tatgttgttt gcctgcatca cgcacaccca ataatgctaa caacaaattt
      121 agcaacacac acatagaatc aaatccattt atttgcttta acagtgatga cggtttgccc
      181 ggtgacgaat ttaaccggca tgaatatttt aaactgccaa atggtgattt gtattttgaa
      241 ggcgtaatgg gtggtgtaca ttttagtcct ttaaaaattt tagaacaaga acccaacgca
      301 cccattgtat taaccaaact gttggtatat aataaacccg ttacttttca aacagacagc
      361 agcatcattc aaatgcctgt atcctatgcc aaaagcatca cattaccacc ggataaaaat
      421 atgtttacca taacgtttgc tgctttaaat tatagttcgg ccagcaaacg actatacaaa
      481 tacatgctcg atggctatga taaacaatgg attgaatcag gactgaagaa tgaagcaact
      541 tatacaaacc ttgccccggg tacttacaca tttaaagtta tacatgcaca aaacaacaac
      601 agcccacaaa acgaaactgc aatacaaata attataaaac cggcctggta tcaaacatgg
      661 ctgtttaaaa taaccttaac aactctttta gcagtcagtg ttattggttt atatcgctac
      721 aggcttaatc agcaaattaa cctgatgcat atacgcaacc gaattgccag cgatttacat
      781 gatgaaatag gcagcacgct aagcagcatt tctatttcaa gtacgattat tcaaaacaaa
      841 atgaatggac acgatagcga tgtaaaaaaa ctactccaac aaataagcat caacacggat
      901 aatatgatgg aggccatgag tgatattgtt tggagcatca acacaaaaaa cgatcgtttt
      961 gacaatatca tcaatcgtat gcgcgcattt gcaatcgaaa tgatggaacc taaaaattgt
     1021 atggttcatt tcaacacaaa aaacataaac gtaaatacga aactagacct gttgcaacgt
     1081 aaaaatttat acctgatttt taaagaagcc attaacaacg ctgccaaata tgctcaatgc
     1141 aaaaatgttt ggattgctat aacaatgcag ggcaataaat taataatgtg tattaaggat
     1201 g
//
LOCUS       DAOMAA010000846         1200 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_152529_length_1200_cov_3.742358, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000846 DAOMAA010000000
VERSION     DAOMAA010000846.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152529_length_1200_cov_3.742358"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /locus_tag="PLO59_10175"
     CDS             complement(<1..1028)
                     /locus_tag="PLO59_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01516.1"
                     /translation="MCKTNKHFIGKILLVCLVLLINVNKSEAQTPIYTQETFVSQIPF
                     NTASYNRRNCTADWLQYKLSGNHGLPNSFEWINNGTYPTAINQNIVGFSTGAGFVRAI
                     NRTVALPAISGAESGDAAILISRSLDFSGHTTFDALRDTIGISMFRDGDGSALQADSV
                     QVYLNTSPQLTGATLLQVACINSNPVGSGITAINRSAFLAPLAFVQSNSWNRFTFGIP
                     NLSDYVSATGGYSAVYLIVAVYSAAGNNIYLDNFNLPQWPTNMVIQSAELTYQENADV
                     GKNTSNNLLANVRIKTRGSLLPLRLDGLTFSTTGSTNFSNDAQNPIAYFTAGTPVFSN
                     TIATGVVA"
BASE COUNT          343 a          233 c          242 g          382 t
ORIGIN      
        1 atggccacta cacctgttgc tatggtattg ctaaaaacgg gtgtgcctgc tgtaaagtat
       61 gcaattgggt tttgtgcatc gttgctaaaa tttgtgctgc ccgttgtgct aaaggttaag
      121 ccatctaatc ttaatggcaa taagcttccg cgtgttttta ttcttacatt ggctaaaagg
      181 ttattgctgg tgtttttgcc tacatcagca ttttcctgat aggttaattc tgcactttga
      241 ataaccatat tggttggcca ttgcggtaaa ttaaagttat ccagataaat gttattgcct
      301 gcggcactat atactgcaac aattaagtac acagcactat aacctccggt tgcacttacg
      361 tagtcagata aattaggaat accaaaagta aaccggttcc aactattgct ttgtacaaat
      421 gccaacggtg ctaaaaaagc cgatcggttt atggcagtta tcccactgcc taccgggttg
      481 ctatttatac aggcaacttg caataaagtt gccccggtta attgtggact tgtgtttaaa
      541 tatacctgaa cagagtcggc ttgcagtgca ctgccatcgc catctctgaa catagaaata
      601 ccaattgtat cgcgcagtgc atcaaatgtt gtatggccgc taaaatctaa cgagcgtgaa
      661 attaaaattg ccgcatcgcc tgattcggca cctgaaattg cgggtaaagc aacagttcgg
      721 tttatagcac gcacaaatcc tgcaccggtg ctaaagccaa caatgttttg gtttatggcc
      781 gtaggatagg tgccgttatt tatccattcg aatgaattgg gtaaaccatg gttacccgat
      841 agtttgtatt gaagccagtc ggctgtacaa ttgcgcctgt tgtagcttgc tgtgttaaat
      901 ggtatttggc ttacaaaggt ttcttgtgtg tagatggggg tttgcgcttc agatttattt
      961 acattgatta ataaaaccag gcacaccagc agtattttgc ctataaaatg cttatttgtt
     1021 ttacacatac cctgagttat cttcactaaa attcatccta atttacttta cgattattaa
     1081 caatgcagaa gtgtttaaac acaatgcatt tcaagcaatt ttgttaataa gtcgggcgca
     1141 aattagtcat tttgttaata acaacacata agttacataa aaattaatag cagcagccta
//
LOCUS       DAOMAA010000847         1200 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_152592_length_1200_cov_2.623581, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000847 DAOMAA010000000
VERSION     DAOMAA010000847.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152592_length_1200_cov_2.623581"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1010)
                     /locus_tag="PLO59_10180"
     CDS             complement(<1..1010)
                     /locus_tag="PLO59_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01517.1"
                     /translation="MFIVFVGVFCWQCNTPSNNRPMPPAEVIDTLKPVNILRFEKDLI
                     QSDTLTATSLAMLRNKYGEFLPLFCKRICPIPMNMANDTILALQLNRFKFDNDIKTIY
                     ASVDSVYPNLNVIAAELAQVFALQKLYLQISKPPAVITFLSAFNYAMITTDTMLLIGL
                     DKYLGSDCPFYPGMNYPAFITKRLRSEYILPNAINAMYQRDYSLDSVKDELLAQVVYY
                     GKMMQYTAHLLPQLADSIILGYSKAELDWCLQNETKIWTALIEQQLLYSTNPKQFLKL
                     INDGPTTQGFPDGAPARLGYFVGWQIVKAYINKTQPDSMQKWLNESDAQKILLQSQYK
                     PN"
BASE COUNT          373 a          227 c          222 g          378 t
ORIGIN      
        1 ttattgggct tgtattgcga ttgcaaaagt attttttgtg catcagattc gttaagccat
       61 ttttgcatac tatcaggttg ggttttgttt atgtacgctt ttacaatttg ccatccaaca
      121 aaatagccca aacgtgccgg ggccccatcc ggaaaacctt gtgtagttgg gccatcgttt
      181 atcaacttta aaaactgttt cggattggtg ctgtataata attgttgttc aattaaagcc
      241 gtccatattt tagtttcgtt ttgcaagcac cagtctaact cggctttact gtagcccaaa
      301 ataatactat cggccaattg tggcaaaaga tgcgcagtat attgcatcat tttaccgtag
      361 taaacaactt gtgctaacaa ttcatcttta acactatcta aactataatc gcgctgatac
      421 atggcattta tagcattggg caatatgtat tcgctacgca aacgtttggt tataaaagcc
      481 ggataattca tgccgggata aaaaggacaa tcgctgccta aatatttgtc taacccaata
      541 agcaacatcg tatcagttgt tatcatggca taattaaagg ctgataaaaa tgtaattacg
      601 gcaggtggtt ttgatatttg aagatacaat ttttgcaagg caaaaacttg tgccaattca
      661 gcagcaatta catttaaatt ggggtacaca ctatctacac ttgcgtatat tgttttaata
      721 tcattatcaa atttaaagcg gttgagttgt agcgctaaaa tggtatcgtt tgccatgttc
      781 atcggaatcg gacaaattct tttacaaaac aaaggcaaaa actccccata cttatttcta
      841 agcatagcca gcgaagttgc tgtaagtgta tcgctctgta ttaaatcttt ttcgaaacgt
      901 agtatattta cgggctttag tgtatcaata acttcggccg gtggcatggg ccgattgttt
      961 gaaggtgtat tacactgcca gcaaaaaaca ccaacaaata ctataaacat aacaaacctt
     1021 ttacttaaca ttgtagccta atttgataaa atatgagaaa acttacttgc ttaatcctgc
     1081 tactttgtgc cagcagccta accaaggcgc aaacctatga aaaatacaaa attggcttgc
     1141 atgcaacgcc aacattccgt tggttaagcg ttgactcgaa aggcgttaaa aacgatggtg
//
LOCUS       DAOMAA010000848         1199 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_152805_length_1199_cov_2.911713, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000848 DAOMAA010000000
VERSION     DAOMAA010000848.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152805_length_1199_cov_2.911713"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="PLO59_10185"
     CDS             <1..216
                     /locus_tag="PLO59_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009184272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HQV01518.1"
                     /translation="EKIIALLDEYVRPAVAQDGGAIDFKSFDNGVVTLTLKGSCSGCP
                     SSTQTLKAGIERLLTQMMPEVTEVVAE"
     gene            255..>1199
                     /gene="eboE"
                     /locus_tag="PLO59_10190"
     CDS             255..>1199
                     /gene="eboE"
                     /locus_tag="PLO59_10190"
                     /inference="COORDINATES: protein motif:HMM:NF035939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metabolite traffic protein EboE"
                     /protein_id="HQV01519.1"
                     /translation="MLLNKFHFSYCTNIHAAQTWDETFNTLQNFLPEIKKQICPHTPM
                     GIGLRLSAVAAQHLLQQALPFFKKWLQQQQLYVFTINGFPYSNFHHTKVKDQVYKPDW
                     TTNERLIYTTNLIHILSGLMQTGTETGISTVPLSYKYWVTDSCNTADVFEACTVQLMK
                     CVLLLHQIDTTQNKYIHIDLEPEPDCLLQNTADTIAYFTNYLLPYGIDLLQAQSGIDA
                     SKARQIILRHITVCYDVCHFAVAFEDADVAISKLKQAGIKIGKVQLSAALKIDKPAQI
                     TAEWIQQKSAAYQAFAESTYLHQTVVKDVNGEMHHYSDL"
BASE COUNT          383 a          256 c          226 g          334 t
ORIGIN      
        1 gaaaaaataa ttgccttgtt agatgaatat gtaaggcctg ctgtggcaca agatggtggc
       61 gctattgatt ttaaatcgtt tgataacgga gtggttacac ttacattaaa aggctcgtgc
      121 agcggctgtc catcatcaac acaaacatta aaagccggta ttgagcgttt gcttacgcaa
      181 atgatgcccg aggtaaccga agttgtggcc gagtaaccgt tttttgcaat caacgtataa
      241 catttaggtt acctatgcta ttaaacaagt ttcacttttc ctattgtact aatatacatg
      301 ccgcccaaac ctgggacgaa acttttaaca cgctacaaaa ttttttgccc gaaataaaaa
      361 aacagatatg cccgcatacc ccaatgggta ttggtttgcg gcttagtgca gtggcggccc
      421 aacatttgtt gcaacaggct ttgccctttt ttaaaaaatg gttgcaacaa caacaattat
      481 atgtttttac cataaacgga tttccttaca gcaactttca ccataccaag gtaaaagacc
      541 aggtgtataa gcccgactgg acaaccaatg aacgtttaat ttacaccaca aaccttattc
      601 acattttatc aggtttgatg caaacaggca ctgaaaccgg tatttctacc gtaccacttt
      661 cgtacaagta ttgggttacc gatagctgca acactgccga tgtgtttgaa gcctgcaccg
      721 tgcaactgat gaaatgcgtg ctgctgctac atcaaattga tacaacgcaa aataaatata
      781 tacacattga tttagaaccc gaacccgatt gcttgttgca aaacacagcc gataccattg
      841 cctactttac taattatctg ttgccttatg gcattgattt actgcaggca caatcaggca
      901 ttgatgcttc aaaagcacgt caaataattt tacgccatat aaccgtatgc tatgatgtat
      961 gccactttgc tgttgctttt gaagatgccg atgtagccat atcaaaatta aaacaagcag
     1021 gtataaaaat tggcaaagtg caactaagtg cagctttgaa aatagacaag cccgcacaga
     1081 ttactgccga atggatacaa caaaaatcag cagcttatca ggcatttgcc gaatcaactt
     1141 acctgcatca aacggttgtt aaagatgtaa acggagaaat gcatcattac agcgatttg
//
LOCUS       DAOMAA010000849         1195 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_153787_length_1195_cov_2.993860, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000849 DAOMAA010000000
VERSION     DAOMAA010000849.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153787_length_1195_cov_2.993860"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1182)
                     /locus_tag="PLO59_10195"
     CDS             complement(<1..1182)
                     /locus_tag="PLO59_10195"
                     /inference="COORDINATES: protein motif:HMM:NF012906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HQV01520.1"
                     /translation="MPAHVPGNVHTDLFQAGIISSLYNSGDDIKNYWIETKNWEYQSV
                     FEVSNKLLQQTHIDLHCKGLDTYAKIFINDIEVATSSNMFLPCITNIKNFVKPGSNTI
                     RILFESPVTKVKSNAIKYGKLPMDNDTSATQVFTRKAAYHYGWDFTPRMVTMGIWQPV
                     FIDAWTSVNINEVALKTLKINSKRARLEATIVMEAPCDEWVQIIIKDYNNQEIEVSKM
                     MVKSSGNDVVKVPVIIENPVLWQTNGLGKQKCYNVDVEVIANNEVAKKSIRTGIREVE
                     VINQSDKAGKNFYIKLNDKPVYIFGANYTPLSVFNGTVTKADYDKLFAALKQSGVNML
                     RIWGGGIYEDDYFYQLADENGILIWQDFMFAGAMYPADSAFIQSVTDEATYQIKRLRN
                     NA"
BASE COUNT          340 a          240 c          185 g          430 t
ORIGIN      
        1 ggcattgtta cgcaaccgtt ttatttgata cgttgcttcg tcagtaacac tttgtataaa
       61 tgcactgtcg gccggataca tggcacctgc aaacataaag tcctgccaaa ttaaaattcc
      121 attttcatca gctaattgat aaaagtaatc gtcttcataa atgcccccac cccaaatgcg
      181 cagcatattt acaccacttt gttttaatgc ggcaaatagt ttgtcgtagt cagccttggt
      241 tacagtgcca ttaaatacac ttaacggtgt ataattggct ccaaaaatgt aaacaggttt
      301 atcattaagt ttgatataaa aatttttgcc tgctttatcg ctttggttta ttacctcaac
      361 ttctctaatg ccggttctaa ttgatttttt ggcaacctca ttgtttgcaa ttacttcaac
      421 atctacatta taacattttt gtttacccaa tccattggtt tgccataaca ccggattttc
      481 aattataacc ggaactttta ctacatcatt accactactc tttaccatca ttttgcttac
      541 ttctatctcc tggttgttat aatctttaat tataatttgt acccattcat cacaaggggc
      601 ttccataact atggtagctt ctaaccgtgc ccttttacta tttattttta atgtttttaa
      661 agctacctca tttatattaa ccgatgtcca ggcatcaata aaaaccggtt gccaaatacc
      721 catcgttacc atgcgtggtg taaaatccca accataatga tatgctgcct tgcgggtaaa
      781 aacctgggtt gcagatgtgt cgttatccat aggcaatttg ccatatttta tggcatttga
      841 ttttactttc gttacaggtg attcgaatag aatccgaata gtattactgc caggtttgac
      901 aaaattttta atgtttgtta tacaaggcaa aaacatatta ctgcttgtgg ctacttcaat
      961 atcattgatg aaaatttttg cataagtatc taatccttta caatgcaaat caatatgcgt
     1021 ttgttgtaaa agtttgttgc ttacttcaaa tacagattga tactcccagt ttttagtctc
     1081 aatccaatag ttttttatat cgtcaccgga attatagagt gatgaaataa tgcctgcttg
     1141 aaacaagtcg gtatgtacat ttccgggtac atgtgccggc atccatggtt cgtta
//
LOCUS       DAOMAA010000850         1194 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_154098_length_1194_cov_2.490781, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000850 DAOMAA010000000
VERSION     DAOMAA010000850.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154098_length_1194_cov_2.490781"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="PLO59_10200"
     CDS             complement(<1..506)
                     /locus_tag="PLO59_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C biogenesis protein"
                     /protein_id="HQV01521.1"
                     /translation="MIQYTGEHLLWGQFGQFFIVLAFTAALLSAIAYLFDTYSKNPNP
                     NWKRLGRVGYVLNGFSVVAIFAILYYLIYNHYFEYYYVWHHSSTTLPMQYIFACFWEG
                     QEGSFLLWTFWHVVLAAVVIRTSKAWESSVMSIIMLVQVFLGSMVLGVWLGDVHIGSN
                     PFILLRNHP"
     gene            complement(575..958)
                     /locus_tag="PLO59_10205"
     CDS             complement(575..958)
                     /locus_tag="PLO59_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015814522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HQV01522.1"
                     /translation="MKRSYIIALIVIALSVGAIMLSIADSSTYATFETAAENPARTYH
                     VIGKVNFDKPFEYNPQQDANLFCFYMTDSIGAEKKVVYHNAKPQDFEKSEQIVVVGKM
                     QGVDFTAEQVLMKCPSKYTEENKTM"
     gene            complement(965..1174)
                     /locus_tag="PLO59_10210"
     CDS             complement(965..1174)
                     /locus_tag="PLO59_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015027967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CcmD family protein"
                     /protein_id="HQV01523.1"
                     /translation="MKLLKLIPAILIFLSQTAIAQTPEMATTMRANGKIYVVVGVLAI
                     IFLGIAIYLFGLDKKLTALEKKIKA"
BASE COUNT          381 a          227 c          217 g          369 t
ORIGIN      
        1 gggtggttgc gcaataaaat aaatgggttg ctgccaatat gcacatcgcc taaccacacg
       61 cctaaaacca tcgagcctaa aaatacctgt accagcatga ttattgacat aacgcttgat
      121 tcccaggctt tacttgtacg tattaccact gcggctaaaa ctacatgcca aaaggtccaa
      181 agtaaaaaac tgccttcctg gccttcccaa aagcaagcaa aaatatattg catgggtaat
      241 gtggttgacg agtgatgcca cacataataa tattcgaaat agtggttgta aataaggtaa
      301 taaagtatgg cgaaaatggc cactaccgaa aacccattaa gcacataacc cacacggccc
      361 aaccgtttcc agttagggtt tgggttttta ctgtaagtat caaataagta tgcaatggcc
      421 gataatagtg ctgccgtaaa tgcaagtact ataaaaaatt gcccaaactg gccccataat
      481 aaatgctcgc ctgtgtattg aatcataaga taatttaaaa aggatttacc atgtttaaaa
      541 atactgtaag cctatgttta caggctgtat ctcattacat ggttttattt tcttcggtgt
      601 atttactggg gcacttcatc agtacttgtt cggctgtaaa atcaacacct tgcattttgc
      661 ctacaacaac aatttgttct gatttttcaa aatcttgcgg tttggcatta tgatacacca
      721 cttttttttc ggcaccaatg ctgtcagtca tataaaaaca aaacaagttt gcatcttgct
      781 gtgggttata ttcaaaaggt ttgtcaaagt ttactttgcc aataacgtga tatgtacgcg
      841 cagggttttc ggcagcggtt tcaaatgttg cataggtgct gctatcggca attgataaca
      901 taatagcacc aacagaaagt gcaattacta ttaaggcaat tatataggaa cgtttcatgt
      961 gtaattatgc tttaattttt ttttcgagtg ccgttaattt tttatccaat ccaaaaaggt
     1021 aaatggcaat gcctaaaaat atgattgcca atacaccaac aactacgtat atcttaccgt
     1081 tagcacgcat agttgttgcc atttcagggg tttgtgcaat tgccgtttga cttagaaaaa
     1141 tcaaaatagc tggtattagt tttaaaagtt tcatgcaaaa attaatcgtt tatt
//
LOCUS       DAOMAA010000851         1193 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_154333_length_1193_cov_2.699473, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000851 DAOMAA010000000
VERSION     DAOMAA010000851.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154333_length_1193_cov_2.699473"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="PLO59_10215"
     CDS             complement(<1..441)
                     /locus_tag="PLO59_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01524.1"
                     /translation="MSDVQPWQFVDDEQDKWPDYIDSIITFSFFTGLISFWWFTNKMQ
                     VNFGTFCKVSILISLIILMSVHIYKRFTSIKWVQTTDVMRTLLSGPLLSILVFSLNYL
                     IVGKPYEVTYTIADIRVVAKNEITDEAVVFFDDNALNDFKTLRTF"
     gene            complement(458..>1193)
                     /locus_tag="PLO59_10220"
     CDS             complement(458..>1193)
                     /locus_tag="PLO59_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018341412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HQV01525.1"
                     /translation="PEKLTWTVATQRNSYWGITVLEKVWPKADSITVNISSHFKPVHA
                     TQNVIGYLPGNISDTFMVVTAHYDHLGGMGDKVFFPGANDNASGVSMLLDVAQHFALL
                     KNRKYSMLFIAFAGEEAGLIGSKYYVDNPLKPLSTIKFLINLDLLGTGDEGLMVVNAV
                     DNMEAFNKLDSINEADKLLPVISKRGKAANSDHYWFGEKKVPAVFLYTMGGIKAYHDV
                     FDIAKTLPLTKYNEVFSLLTTFIKTF"
BASE COUNT          391 a          234 c          189 g          379 t
ORIGIN      
        1 aaaagttctg agggttttaa aatcgtttaa ggcattatca tcaaaaaaaa ccacagcctc
       61 atcggtaatt tcattttttg ctacaacacg aatatctgca atggtgtatg ttacttcata
      121 aggcttaccg actattaaat aatttaaaga aaaaaccaaa atgcttaaaa gcgggccgct
      181 taatagtgta cgcattacat cggtggtttg tacccattta attgatgtaa agcgtttgta
      241 tatatgcaca ctcattaaaa ttattaatga tattaaaatg ctcactttac aaaaagtacc
      301 aaagtttacc tgcatcttat ttgtaaacca ccaaaacgaa ataagacctg taaaaaaact
      361 aaatgtaata atagagtcaa tgtaatcggg ccacttgtct tgctcatcat ctacaaattg
      421 ccaaggttga acatcgctca ttaagacctg atacttttta aaacgtttta ataaatgttg
      481 tcagcagact aaaaacttca ttgtatttgg taagcggtaa agttttagct atatcaaaca
      541 catcgtgata agcttttatg ccacccatag tatataaaaa cacagcaggt actttttttt
      601 cgccaaacca gtagtggtcg ctattggcgg ctttgcctct cttgctaatc actggtaata
      661 gtttatcagc ttcgttaatg ctatcaagtt tattaaaagc ttccatatta tcaacagcat
      721 tcactaccat gaggccttca tcgcccgtac ccaataaatc aagattaatt aaaaacttaa
      781 tggtgcttag tggcttcaaa ggattatcta cataatattt agagccaatt aatccggctt
      841 cttcgcctgc aaatgcaata aataacatgc tgtacttcct gttttttaaa agggcaaaat
      901 gttgtgctac atcaagcagc atacttacac cgcttgcatt gtcgttagca cccggaaaaa
      961 aaactttatc gcccatacca cctaaatgat cgtagtgtgc tgttaccacc ataaacgtat
     1021 cactaatatt acccggcaaa tagccaatta cattttgcgt tgcatgtaca ggtttaaaat
     1081 gtgatgaaat atttactgta atactatcgg ctttaggcca aactttttcg agaactgtaa
     1141 tgccccaata actattgcgt tgtgttgcta ccgtccaggt tagtttttcg ggt
//
LOCUS       DAOMAA010000852         1193 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_154338_length_1193_cov_2.659051, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000852 DAOMAA010000000
VERSION     DAOMAA010000852.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154338_length_1193_cov_2.659051"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /locus_tag="PLO59_10225"
     CDS             <1..1009
                     /locus_tag="PLO59_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020532802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HQV01526.1"
                     /translation="LQVFEGLTSYIFKNIHQINLPNFERISYQTAMNLYGNDKPDMRF
                     EMKFVDLKSTVIGRNFKVFDDASYVVGIVAPKCANYTRKQLDELTDFVKRPQIGATGL
                     VYIRCNDDGSIKSSIDKFYSENDLQSIAQTSNAKAGDLILILAGALNKTQKAMSELRL
                     EMGQRLGLRTPGVYKPIWVVDFPLLEFNEEQNRFFAMHHPFTSPKTDDFHLLESNPGA
                     VRANAYDLVINGVEIGGGSIRIHDKQLQNKMFNLLGFTPEEAQEQFGFLLNAFEFGAP
                     PHGGIAFGLDRLCSMIGGTDSIRDFIAFPKNNAGRDVMIDAPSAIDQKQLSELSIAVI
                     K"
BASE COUNT          361 a          231 c          247 g          354 t
ORIGIN      
        1 tttacaggtg ttcgagggac ttacgtccta tatttttaaa aacatacatc aaattaactt
       61 gccaaacttt gaacgcatca gttatcaaac agccatgaac ttatatggca acgataagcc
      121 cgacatgcgt tttgaaatga aatttgtgga tttgaaatca accgttatcg ggcgcaattt
      181 taaagtgttt gatgatgcgt catacgtagt aggtattgtt gctcccaaat gtgctaacta
      241 tacacgtaaa caattagatg agttaaccga ttttgtgaaa cgaccgcaaa ttggcgcaac
      301 cggtttggtt tatatcagat gcaatgatga tggcagcata aaatcatcaa tagataaatt
      361 ttacagtgaa aacgatttac aaagcattgc acaaacaagt aatgcaaaag caggcgattt
      421 aattttaata cttgccggtg cacttaacaa aacgcaaaaa gccatgagcg aactcaggct
      481 cgaaatgggt cagcgtttag gtttacgcac ccccggagtt tacaaaccca tttgggtggt
      541 tgattttccg ttgttggaat ttaacgaaga gcaaaaccgt ttttttgcca tgcaccatcc
      601 ttttacttcg cctaaaaccg atgactttca tctactcgaa agcaaccccg gtgctgttag
      661 agccaatgct tacgacctgg taattaatgg cgtagaaata ggcgggggct ccataagaat
      721 acacgataag cagttgcaaa ataaaatgtt taacctgttg ggttttacgc ccgaagaagc
      781 acaagagcag tttggttttt tgctaaatgc atttgagttt ggtgcaccac cacatggcgg
      841 tattgcattt gggttagata gattatgcag tatgattggt ggcactgata gcatcagaga
      901 ttttattgca tttcctaaaa acaatgctgg ccgcgatgta atgattgatg cgccttcggc
      961 tatagatcaa aaacaattat ccgaactcag tatagcagta attaagtaag cagcaattat
     1021 cactggctaa aatgtaatgc ttttaaccat tgtttcctgc ccacgtttta tggttacgtt
     1081 ggttatatcg cctttgctaa attggcccag ggctttcatg tagctgttca tgtccgacac
     1141 cgttgtttcg ccaatctgta aaacaatatc gcctttaagt aaaccagcgt ttg
//
LOCUS       DAOMAA010000853         1193 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_154419_length_1193_cov_2.047452, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000853 DAOMAA010000000
VERSION     DAOMAA010000853.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154419_length_1193_cov_2.047452"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="PLO59_10230"
     CDS             complement(<1..299)
                     /locus_tag="PLO59_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01527.1"
                     /translation="MKKNIQKKTVASSEKSVKKIKEAPTVGLLEKYLPLCILLAVIFL
                     AYMPCLDNDLTNWDDPTYLNENPLIRSLDAANIKRIFTENYFGNYQPLHILSYAV"
     gene            complement(313..753)
                     /locus_tag="PLO59_10235"
     CDS             complement(313..753)
                     /locus_tag="PLO59_10235"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018478980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQV01528.1"
                     /translation="MMCVIKLSGVTDVNAKMLFEIRKKVFVEEQHVPATLEFDKFENE
                     ANHYLCFDGDKPCGTARWRITQNGIKLERFAVLPAHRNKGIGAVILNQVLNDIAPLKQ
                     KVYLHAQAGAVNFYLKHQFVVDGNCFFEANIPHYVMIHQPIKTI"
     gene            complement(753..>1193)
                     /locus_tag="PLO59_10240"
     CDS             complement(753..>1193)
                     /locus_tag="PLO59_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01529.1"
                     /translation="FIVCLVIILFYQSCKIKDVTYKRIENLNFELRNAVPNINFDIVC
                     YNPNAMGCRIGQLHCEILSNTDSVAYAYNIETLKVRPYSEFSVPVKSIVSAKAAFKLT
                     GKSLLSTKDIPIRLVGHMKIKKFIFSHTYYFDVTESFNKSQLYK"
BASE COUNT          387 a          206 c          198 g          402 t
ORIGIN      
        1 tcgacagcat atgacaaaat gtgcaatggt tgataatttc caaaataatt ttcagtaaaa
       61 atgcgcttta tgtttgctgc atccagcgag cggattaatg ggttttcatt cagatacgtt
      121 gggtcatccc agttggttaa gtcattatct aaacaaggca tataagccag aaaaattact
      181 gccaataaaa tgcaaagcgg caaatacttt tcgagcaaac caacggttgg tgcctcttta
      241 atttttttta ctgatttttc agatgatgca acggttttct tctgaatatt ttttttcatg
      301 aaaatgaata aactaaattg ttttaattgg ttgatgtatc ataacatagt gcggaatatt
      361 agcttcaaag aagcaattgc catcaactac aaactgatgc tttaaataaa aatttacggc
      421 accggcttgt gcatgcaggt aaactttttg ctttagtggt gcaatgtcgt taagtacctg
      481 atttaaaata acggcaccta tgcctttgtt tctatgggca ggcaatacag caaaacgttc
      541 gagttttatg ccgttttggg taatgcgcca tcgtgcagta ccacaaggct tatcaccatc
      601 aaaacataaa taatgattag cctcattttc aaatttatca aactctaatg ttgcaggcac
      661 gtgttgctct tcaacaaaaa cctttttacg gatttcgaat agcatttttg catttacatc
      721 agttacacct gaaagtttta ttacgcacat cattatttat aaagttgtga tttattaaat
      781 gactcggtta catcaaaata atatgtatga ctgaatataa atttcttgat tttcatgtgg
      841 ccaactaatc gtatgggtat atctttagtt gatagtaaac tcttgccggt taatttaaag
      901 gctgctttgg ccgatacaat actttttaca ggcacagaaa actcgctgta aggacgaact
      961 tttaaagttt cgatattgta tgcataagcc acactatctg tattacttaa aatttcgcaa
     1021 tgcaattggc caatacggca tcccatagca ttggggttat aacacacaat atcaaaattt
     1081 atatttggta cagcgttacg caattcaaaa ttcaaatttt caattcgttt ataggtaaca
     1141 tcttttatct tacatgattg gtaaaataaa ataataacca agcacactat aaa
//
LOCUS       DAOMAA010000854         1192 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_154692_length_1192_cov_1.839050, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000854 DAOMAA010000000
VERSION     DAOMAA010000854.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154692_length_1192_cov_1.839050"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1192)
                     /locus_tag="PLO59_10245"
     CDS             complement(<1..>1192)
                     /locus_tag="PLO59_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01530.1"
                     /translation="NPVVVYNTPGTYTVTLTQSNSCGTTSTSQTMHIKAKPVLNPVFT
                     NYNGCTPDTMGVIVNSQNTAGYAWLWQVNPAGVSISNPNDSQPQFIALNTNTYGFQVQ
                     VGDMICCSPFFSGSWCLWNDSITFLQGSSALYTPIADTCVAWPVNYALNLSNHVTFSP
                     FNLDSSWSLLVNGMPVLINSAGIAQITDTGHYQFNIQVSNTCDTLMYAYNFVVSTPPP
                     NPNIKTDSTVCINALPIDLNIISNNLNGTWTWNGNILQPALFNPGSTQTATNNLIFTT
                     GPTGCDVSDTIIVNVVGLGITAGNDIDLCSNSDTVNISGGALPATGFWQGNGIVSNTG
                     IFNPDSATANPAIVTYTINTGGCTIIDTLLITLSSPPAAAFNIPTCICVNTPVLFEDT
                     IANT"
BASE COUNT          349 a          227 c          250 g          366 t
ORIGIN      
        1 atggtgtttg ctatggtatc ttcgaacaag acaggtgtat ttacgcaaat gcatgtgggt
       61 atattaaacg ctgcggcagg tggactgcta agtgttatca gcaatgtatc aataatggta
      121 cagccgcctg tatttatagt atatgttaca attgcaggat tagctgtggc cgaatcggga
      181 ttaaatattc ccgtattact aacaatacca ttgccttgcc aaaaacccgt tgccggtaat
      241 gcgccaccac ttatgtttac agtatcacta ttactgcata aatcaatatc gttaccggct
      301 gtaatgccta aaccaacaac gtttacaata atggtatcag atacatcaca acctgttggc
      361 cctgttgtga aaattaaatt attggttgct gtttgagttg aacccgggtt gaacaaagcc
      421 ggttgcaaaa tgtttccatt ccaagtccat gtgccattta agttgttcga aataatattt
      481 aaatcaatag gcaaggcatt tatacaaacc gtgctatctg ttttaatatt tggattgggt
      541 ggtggtgtgc ttacaacaaa attataggca tacattaatg tatcgcatgt gtttgaaacc
      601 tgaatattaa actgataatg acccgtatcg gtaatttgtg ctatgccagc actattaata
      661 agcacaggca ttccatttac caataaactc caactgctat ctaaattaaa tgggctaaaa
      721 gttacatggt tgcttaggtt taatgcgtag ttaaccggcc atgccacaca agtatcggca
      781 atgggtgtgt acaaggcact tgagccttgt aaaaaggtta tgctatcatt ccataaacac
      841 caactacccg aaaaaaacgg gctgcagcaa atcatatcgc ctacctgcac ctgaaagcca
      901 tacgtattgg tgttaagtgc aataaattga ggctggctgt cgttggggtt ggatatgctt
      961 acacctgccg gattaacttg ccaaagccat gcataaccag cggtgttctg actgtttact
     1021 attacgccca ttgtatcagg tgtgcaacca ttataattgg taaataccgg atttaaaacg
     1081 ggttttgctt ttatatgcat ggtttgcgat gtgctggtgg taccgcatga attgctttgt
     1141 gttaatgtta ctgtataggt acccggtgta ttataaacta cgacaggatt ag
//
LOCUS       DAOMAA010000855         1188 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_155573_length_1188_cov_2.730803, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000855 DAOMAA010000000
VERSION     DAOMAA010000855.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155573_length_1188_cov_2.730803"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="PLO59_10250"
     CDS             <1..577
                     /locus_tag="PLO59_10250"
                     /inference="COORDINATES: protein motif:HMM:NF015926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HQV01531.1"
                     /translation="NQKLWLRFKTACDTFFNRKSQHFSSLDSEQENNYTAKMKLIESV
                     ELFNLGENNQANLDQLKEFQRQWSAIGLVPLKKKDEVHQRFRQAIDKLYDHLKIDERE
                     KMRSRYQTDDAQNRSYKQNNAKPRTETGVLSSKISDLANEVTVWQNNIGFFSKSKNAD
                     VIRKEFEDKIIAAKKEIDSLKQKLKALKETE"
     gene            complement(617..931)
                     /locus_tag="PLO59_10255"
     CDS             complement(617..931)
                     /locus_tag="PLO59_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018676630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01532.1"
                     /translation="MMLFYFKWLPVGGIVLFPFVLMHVSQKHNAITLNHERIHYRQQL
                     EMCILPFYICYVLHYFYNLIRYRKHQMAYTQICFEREAYANQTNQRYLEQRKFFAWYK
                     YI"
     gene            complement(933..>1188)
                     /locus_tag="PLO59_10260"
     CDS             complement(933..>1188)
                     /locus_tag="PLO59_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01533.1"
                     /translation="TFNASLFILVAGGLGMTAPVQGGIGAYHLLVSAALVLFGVAYED
                     GLAFATLLHTAQMLQIVVLGLLALLALTLMPQATGKKTVV"
BASE COUNT          451 a          217 c          205 g          315 t
ORIGIN      
        1 gaatcaaaaa ttatggcttc gttttaaaac tgcttgcgat acatttttta atcgtaagag
       61 ccagcatttt tcttcgctcg atagcgaaca ggaaaataat tatacagcta aaatgaaact
      121 gattgaatcg gttgaacttt ttaatctggg cgaaaacaat caagctaatt tagatcagct
      181 taaagaattt caacgtcaat ggtcggctat tggcttggtg ccattaaaaa agaaagacga
      241 agtacatcaa cgttttaggc aagctattga taaactttac gatcatttaa aaatagacga
      301 acgcgagaaa atgcgatcac gatatcagac tgatgatgct caaaatcgta gctataaaca
      361 aaacaatgcc aaaccacgaa ccgaaaccgg agtgcttagc tcgaaaataa gtgaccttgc
      421 aaatgaggtt acggtatggc aaaataatat tggctttttc agcaaatcaa aaaatgcaga
      481 tgttatacgt aaagaatttg aagacaaaat tattgctgcc aaaaaagaaa ttgacagttt
      541 aaaacaaaaa ctcaaagcgt taaaagaaac cgaataaaaa atgcctccat aacggaggct
      601 tttttatgct taaaacttat atatacttat accaggcaaa aaactttcgt tgctccaaat
      661 accgttggtt ggtttgattg gcatacgctt cgcgttcaaa acaaatttgg gtataggcca
      721 tctgatgttt tctatagcga attaaattat aaaaataatg caacacataa caaatgtaaa
      781 aaggcaaaat gcacatttct aattgttgcc tgtaatgtat gcgttcatga tttaatgtga
      841 tggcattatg tttttgtgaa acgtgcatta aaacaaaagg gaataacacg ataccaccaa
      901 ccggtaacca tttaaaataa aatagcatca ttttaaacca ccgttttctt accggttgct
      961 tgcggcatta aggttaaagc aagcaatgcc aataaaccca atacaactat ttgcaacatt
     1021 tgtgcagtat gtaacaaagt ggcaaatgcc aaaccatctt cgtaagctac accaaacaaa
     1081 accaatgcag ccgacaccaa taaatgatag gcaccaatgc caccttgtac aggtgcagtc
     1141 atacccaaac caccggctac taaaataaaa agcgatgcat taaaggtg
//
LOCUS       DAOMAA010000856         1187 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_155874_length_1187_cov_2.327739, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000856 DAOMAA010000000
VERSION     DAOMAA010000856.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155874_length_1187_cov_2.327739"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1187
                     /locus_tag="PLO59_10265"
     CDS             <1..>1187
                     /locus_tag="PLO59_10265"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HQV01534.1"
                     /translation="QGVTMGTNNLFSGQLQNVLNSTTFESNAPLIAPHAGKKPEVFLD
                     LNGIQNIDFVNENHFTKGSYGIKAVDCRFNLSDATFTEVDFGIESKVMSPTVTTGHEF
                     HNNRFYKYFTAIRIDNGLNDKIGTNLFNPATHNGTQEQNYIGISLQACNGFLLTDNIF
                     YKNKYGIMVHKSGLLNNRISTNTGTGNQFISCWRGIHTTGTNPSLQIKCNYFQNNQAS
                     TQYSAAWYVHDLLCNQGSNNQSDPQSPAGNRFYHAPASRVDLVSKQGSGPNGLNFIYY
                     HHSPNGHPEVKPTVNNANWIQLVNKNIAYDQLTACTGITLMVLADNSPDTAKLIIDNT
                     TDSILARQYANELVQWYIIHNMDSAAIDYLNTNNLGLDERLLFSLYAEHGAYTETTDL
                     LAQ"
BASE COUNT          390 a          261 c          229 g          307 t
ORIGIN      
        1 cagggagtaa cgatgggaac gaataattta ttcagcggac aattgcaaaa tgtactaaac
       61 tccaccactt ttgaaagcaa tgcacctttg attgctcccc atgccggaaa aaaaccggag
      121 gtgtttttgg atttaaacgg aatacaaaac atagattttg ttaacgaaaa ccattttaca
      181 aaaggtagct atggcataaa ggctgtggac tgcagattta acctttccga tgcaacgttt
      241 accgaggtgg actttggaat tgaatctaaa gtgatgagtc ctactgttac tacaggccat
      301 gagtttcata ataatagatt ttataaatat tttactgcca tacgtattga taacggattg
      361 aacgataaaa taggaacaaa tttatttaac ccggcaaccc ataacggtac acaggaacaa
      421 aactatattg gcataagcct gcaggcctgc aatggatttt tgttgaccga caatatattc
      481 tacaaaaaca aatacggcat catggtacac aaatcaggct tgcttaacaa ccgcataagt
      541 accaacaccg gaaccggaaa tcagtttatt tcgtgttggc ggggtatcca tactacaggt
      601 actaacccaa gccttcaaat taagtgcaac tactttcaaa acaatcaagc atctacgcag
      661 tatagtgcag catggtatgt acacgattta ttgtgcaatc agggttctaa taaccaaagt
      721 gatccacaat cccctgcggg caaccgtttt tatcatgcac cggcctcgcg ggttgatttg
      781 gtttctaagc aaggcagtgg ccccaacggc ttaaacttta tatattacca ccacagtccc
      841 aatggacacc ccgaggtaaa gcctaccgtt aacaatgcaa attggataca acttgtaaac
      901 aagaatattg cttacgatca actcactgcc tgtacaggca tcacgttaat ggtactggcc
      961 gataacagcc ccgatacagc caagctgata attgacaata ccaccgatag catactggcc
     1021 cggcagtatg ccaatgaatt ggtacagtgg tatattatcc ataacatgga ctcggcagcc
     1081 atagactacc tcaataccaa caaccttgga ttagatgaaa ggttattatt tagcctatat
     1141 gctgaacacg gtgcctatac cgaaaccact gatttattgg cgcaaat
//
LOCUS       DAOMAA010000857         1186 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_156118_length_1186_cov_2.520778, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000857 DAOMAA010000000
VERSION     DAOMAA010000857.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156118_length_1186_cov_2.520778"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1186)
                     /locus_tag="PLO59_10270"
     CDS             complement(<1..>1186)
                     /locus_tag="PLO59_10270"
                     /inference="COORDINATES: protein motif:HMM:NF013636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HQV01535.1"
                     /translation="SDGFTSTDANPSHNFALTGSYTITLVATDSCGTDSTSQQVSIVT
                     YAPATCAKSFDFTTVATPITDNDVTGITLPLNINNVVGQLGTDVSLVSICINGTHTNV
                     GDLKMELIAPNGKVVQLIDQPGYPAGTTGCTSDDFNFCIERGIGADAENACNATAPAI
                     SGPYTAMNGYNLDSINIAGGAANGLWQLVVYDLLPGETGEVLNAQLVFDNQPQANANF
                     ASSINAYTVTLTAANIPNTTYTWSLGDGNTASGSSVIHNYISNGAYVITLTATDSCGS
                     VSTIQGVIINEPITCERTYTYAPVPQSIPDNDSNGVVLSYNLNGVSGNNLGVDVRLTG
                     VCVSGTHSRVGDVAINLTAPNLKSVSLMDRPGYPATTMGCATPNFDFCILGGIGLDNE
                     AAC"
BASE COUNT          364 a          250 c          222 g          350 t
ORIGIN      
        1 tacaagctgc ttcattgtct aaaccaatgc cacctaaaat acaaaaatca aagtttggag
       61 tagcgcaacc catagtagtt gcagggtaac cgggtctatc cattaacgaa actgatttca
      121 aattaggtgc cgtaagattt attgccacat cgcctaccct actgtgtgta ccactaacac
      181 aaacacctgt taaacggaca tcaactccta aattatttcc tgaaacacca tttaaattat
      241 agcttaatac aacaccgttc gaatcgttat caggtatgct ttgaggtacc ggtgcatagg
      301 tataagtacg ttcgcaggta ataggttcgt ttattattac accttgaata gtacttacgc
      361 ttccgcagga atcggttgca gtaagcgtta ttacataagc accattacta atataattat
      421 gaattacact actacctgaa gcggtattac catcgcctaa actccatgta taagttgtat
      481 taggtatgtt agctgctgtt aatgttactg tatatgcatt aatggatgaa gcaaaattgg
      541 cattagcctg aggttgatta tcaaatacaa gttgtgcgtt taagacctca ccagtttcgc
      601 ccggcaataa atcatataca accagttgcc ataaaccatt ggcggcacca cctgcaatgt
      661 ttatcgaatc gaggttgtaa ccgttcatag ctgtataagg acctgaaata gcaggtgctg
      721 ttgcattaca tgcattttcg gcatcggcac ctatgccacg ttcgatacag aaattaaaat
      781 catcactcgt acaaccagtt gtaccggctg gatagcccgg ctgatcaata agttgaacca
      841 ctttgccatt aggggcaata agctccattt ttaaatcgcc tacattggta tgtgtaccat
      901 taatacaaat acttaccaaa ctaacatcgg tacctaactg accaactaca ttatttatgt
      961 ttaatggcaa tgttataccc gttacatcat tatctgttat tggtgttgct acagtagtaa
     1021 aatcgaacga cttagcgcaa gtagcaggag cgtatgttac aatactaact tgttgtgatg
     1081 tagagtctgt accgcaacta tcggttgcaa ctaaggtaat tgtatagcta ccggttaatg
     1141 caaaattatg cgaagggttg gcatcggtac tcgtaaaccc atcgct
//
LOCUS       DAOMAA010000858         1185 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_156349_length_1185_cov_2.694690, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000858 DAOMAA010000000
VERSION     DAOMAA010000858.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156349_length_1185_cov_2.694690"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /locus_tag="PLO59_10275"
     CDS             complement(<1..826)
                     /locus_tag="PLO59_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01536.1"
                     /translation="MQFSWAKSFNGNGTQLLPKKIVTDNAGNSYVAGCFSGTQTFDTV
                     SIVAKGTTDAYLAKFNATGNCLWARTYGFSISQTYGTCVAIDAAGNVILGGNFTNYFN
                     IDTSYTISLGQTDMFMIKHDTQGNLIKFNTAGGTSTDNLNTITTDMQGRYYIGGSFGF
                     KCFFGNDSIQTPNLSPLSDIFLAAYDVNMQPVWAKRAGGASDNDVAKSIQFRNNSIYL
                     AGIIIGNNCTFGTNSVNSYGQSDAVVAKYALDGTNQWVKRAGGTANDYCNALSTDSF"
     gene            complement(917..>1185)
                     /locus_tag="PLO59_10280"
     CDS             complement(917..>1185)
                     /locus_tag="PLO59_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01537.1"
                     /translation="IISLYVDYKKSLPENEQIVSKFSGKKMKTVGNKWSDLEASIYNK
                     NSQPYYVLVDHNGNLLANPIGFTDTETYNTFLEEGKCRFKKRLQ"
BASE COUNT          370 a          215 c          219 g          381 t
ORIGIN      
        1 caaacgaatc ggtacttaat gcattacaat aatcgttggc tgtaccaccg gcacgtttaa
       61 cccattggtt agttccatct aaggcatact tggctactac tgcatcagat tggccatagc
      121 tgttaacact attagtaccg aatgtacagt tattgccaat aataataccg gctaaataaa
      181 tgctgttatt acgaaattga attgattttg ctacatcatt atcagatgca ccacctgcac
      241 gtttagccca aaccggctgc atatttacat cataggctgc taaaaaaata tcggataagg
      301 ggcttaggtt tggtgtttga atactatcat ttccaaaaaa gcatttaaaa ccaaacgaac
      361 cgccaatgta ataacggcct tgcatatctg tagttattgt gtttaagtta tcggtagaag
      421 taccgcccgc agtattaaac ttaatcaaat tgccttgtgt atcatgttta atcataaaca
      481 tatcggtttg gcctaaggaa attgtatagc tggtatcaat attaaaatag ttggtaaaat
      541 tacctcctaa aataacattg cctgctgcat caatggcaac acatgttcca taggtttgcg
      601 aaatactaaa gccataagtg cgtgcccata agcaattacc ggtggcatta aatttagcta
      661 aataggcatc ggtagtacct ttggcaacaa tacttacggt atcaaaagtt tgggtgcccg
      721 aaaaacagcc agccacgtaa ctatttccgg cattgtcggt tacaattttt ttaggtagta
      781 gttgtgtgcc attaccatta aaacttttag cccacgaaaa ctgcatttgg ctataactaa
      841 aatgggtaag caataaaaaa agtaaagtgt aaattattct catggtgtgt gaatttggat
      901 ggggattata gttcaattat tgaagtcttt ttttaaaccg gcacttgcct tcttctaaaa
      961 aagtattgta ggtttcggta tcggtaaagc caatggggtt tgccagcaaa ttaccgttat
     1021 gatcaactaa aacataatat ggttgtgagt ttttattata aatgctggct tctaaatcgc
     1081 tccatttatt acccacggtt ttcatttttt tgcctgaaaa cttagatacg atctgttcgt
     1141 tttcgggcaa ggattttttg taatctacgt acaaggaaat tatta
//
LOCUS       DAOMAA010000859         1184 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_156640_length_1184_cov_2.534987, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000859 DAOMAA010000000
VERSION     DAOMAA010000859.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156640_length_1184_cov_2.534987"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1184)
                     /gene="glgP"
                     /locus_tag="PLO59_10285"
     CDS             complement(<1..>1184)
                     /gene="glgP"
                     /locus_tag="PLO59_10285"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016868216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005977 - glycogen metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HQV01538.1"
                     /translation="QEVEPGLGNGGLGRLAACYLDSMTTLQVPAIGYGIRYEFGIFEQ
                     QIQNGQQIEITDKWLQNGNPWEIVQHNITYKIKFGGYTQTVSKPNGQYEVQWIAQDEV
                     KGIAYDTPVPGYKNNAINLLRLWKAEAFEALDLSAFNHGNYGIAVQKKTWAETISKVL
                     YPNDETEDGKRLRLMQQYFFVSCSLQNVIGIQLQRNKTLHDLPQTFALQLNDTHPAIS
                     IAEMMRLLVDEHQMWWDEAWKITQAVFSYTNHTLLPEALEKWPLQLFTALLPRHIEII
                     YEINSRFLESVAQQHPGNNTLLSNLSIIDETGGKAVRMAHLASIGSHTINGVSQLHSK
                     LLKEDLLADFAKIKNKNIINVTNGVTPRRWMALNNPMLAKLISDYIGNAWIGNTQEQL
                     RK"
BASE COUNT          293 a          231 c          239 g          421 t
ORIGIN      
        1 attttcgtaa ttgttcttgt gtattaccaa tccatgcatt gcctatgtag tcgcttatta
       61 attttgcaag catcggattg tttaatgcca tccacctgcg tggcgttaca ccattggtta
      121 cattaataat gtttttgttt ttaatttttg cgaagtcggc cagtaaatct tctttcaaaa
      181 gttttgaatg cagttgcgat acaccattta tagtgtggct gcctatggat gccaaatgtg
      241 ccatacgaac ggctttgcca ccggtttcgt caataatgga tagattgcta agtaatgtat
      301 tgttgccggg atgttgttgc gcaaccgatt ctaaaaatcg gctgtttatt tcataaataa
      361 tttcaatatg gcggggtaat agtgccgtaa ataattgaag tggccatttc tctaatgctt
      421 cgggcaataa ggtatgattg gtatatgaaa atacggcttg tgtaattttc caggcttcgt
      481 cccaccacat ctgatgttcg tcaaccagta gtcgcatcat ttctgctatc gagatggcag
      541 ggtgtgtatc gtttaattgc aatgcaaaag tttgtggcaa atcatgcaat gttttattgc
      601 gttgtaactg tatgccaatt acattttgta acgaacatga aacaaaaaag tattgttgca
      661 tcaggcgcag gcgtttcccg tcttcggttt catcgttagg gtaaagtact tttgatatgg
      721 tttcggccca ggtttttttc tgcacggcaa tgccataatt accgtgatta aatgcactta
      781 aatccaatgc ttcaaatgct tcggctttcc ataaacgcaa taggtttata gcattgtttt
      841 tatatccggg tacgggtgtg tcataagcaa taccttttac ttcatcttgc gctatccatt
      901 gcacttcgta ttgtccgtta ggcttgctta cggtttgggt ataaccacca aattttattt
      961 tgtaagttat attgtgttgt actatttccc agggattgcc attttgtagc cacttatcag
     1021 ttatctctat ttgctgtccg ttttgaattt gttgttcaaa tatgccaaac tcgtatctga
     1081 tgccgtaacc aattgccggc acttgtagtg tggtcatact atctaaataa catgctgcca
     1141 aacggcctaa gccaccattg cctaagcccg gctcaacttc ttgt
//
LOCUS       DAOMAA010000860         1183 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_156894_length_1183_cov_2.557624, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000860 DAOMAA010000000
VERSION     DAOMAA010000860.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156894_length_1183_cov_2.557624"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..>1183
                     /locus_tag="PLO59_10290"
     CDS             114..>1183
                     /locus_tag="PLO59_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HQV01539.1"
                     /translation="MKKLLLKTVISLCTVCWLNTTAQNQNPKTIAETQNAANPEKVTC
                     AFSFNDVTVDSIEGCSGPFAPYTLTVHYTGTCPNVTMQIKYNDMNGNDAYMPFNKSGN
                     DFSINVVNDATGQDLVLIGTDGNSSDSMLFELYTIPCEVSISYNVLYTNCGANIYSNF
                     SSNFCWPLDNYFRLKKLNSNTEIINTTSSSIYSVPNGTYRIEIHDIGGCSTGWDTIVI
                     AGPDRAAPTNLSAVQVGSQNKVKIKWEDNCGYSDIPLDFFQIKYRIKGNNTWSFKIIT
                     HPKNTNSWDKYYTKIDNIILNTVYEYRISSTVLDDKSLWSPIHEFCIGGGCAARTVSE
                     TDVDESNDAIAVFPNPAKDVIH"
BASE COUNT          411 a          223 c          214 g          335 t
ORIGIN      
        1 gcagccaatt aaacatttgg ctatccggca cggtaaagtg catgtacgtt tgttatgcgt
       61 ttaataaaat acgctgcata caaaatcatt ttcactttta aatccttatc gtcatgaaaa
      121 agctactact aaaaacagtc ataagtttgt gtaccgtatg ctggttaaat acaacagcac
      181 aaaaccaaaa tcctaaaaca attgcagaaa cacaaaacgc agcaaatccc gaaaaagtaa
      241 cctgtgcctt tagttttaat gatgtaacag tagattcaat tgaaggttgt tccggccctt
      301 ttgctccata tacgcttact gtgcattata ccggtacctg ccccaatgtt accatgcaaa
      361 ttaaatataa tgatatgaat ggtaatgacg catatatgcc atttaataaa tccggcaacg
      421 actttagtat taacgtagtc aatgatgcaa ccggtcagga tttggtttta attggcactg
      481 atggcaatag tagcgatagt atgctgttcg aactatatac gataccttgt gaggttagca
      541 tcagctacaa tgttttatat acaaattgtg gcgcaaacat atacagcaat tttagcagta
      601 acttttgctg gccattagac aactatttca gacttaaaaa actgaatagc aatacagaaa
      661 taataaatac aacaagttca tcaatttaca gcgtgccaaa tggaacttac cgaattgaaa
      721 tacatgatat tggaggctgc agcaccggtt gggacacaat tgttattgcg ggccctgaca
      781 gagccgcacc tacaaacctt tcggcagtgc aagttggtag tcaaaacaaa gtaaaaatta
      841 aatgggaaga taactgcggt tactcagata taccattaga tttttttcag attaaataca
      901 gaataaaagg aaacaataca tggagtttta aaattataac acatcccaaa aacaccaaca
      961 gttgggacaa gtattataca aaaattgaca acatcatcct taacaccgtt tatgagtatc
     1021 gtatctcatc aactgtattg gatgataaaa gtctttggtc gcccatacat gagttttgca
     1081 ttggtggtgg ctgtgctgca cgcaccgttt cagaaacgga tgttgatgaa agcaatgatg
     1141 caatagccgt atttcccaat ccggcaaaag atgttataca ttt
//
LOCUS       DAOMAA010000861         1183 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_156989_length_1183_cov_1.959220, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000861 DAOMAA010000000
VERSION     DAOMAA010000861.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156989_length_1183_cov_1.959220"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="PLO59_10295"
     CDS             complement(<1..350)
                     /locus_tag="PLO59_10295"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HQV01540.1"
                     /translation="MRKIVLAHLAVLTANIIYGINFSIAKDVMPAFIGPFAFILIRVV
                     SAAILFLIIHLFFIKQKVARKHLFLLMQCALFGVAINQLLFFKGLSLTQPINAALVMC
                     TNPIQVVLLSAMLG"
     gene            492..872
                     /locus_tag="PLO59_10300"
     CDS             492..872
                     /locus_tag="PLO59_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015332311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HQV01541.1"
                     /translation="MALDITGKIIHILPEAGGQTKAGKPWSKQEFVIETEEQYPKKVC
                     ISLMGEKVQELKKFSIGQQVKASLNIESREYNGRWYSDIRAWRLEAAGSTQPQSNYAP
                     PANTPADSFSSNPLPAATDDDLPF"
     gene            complement(931..>1183)
                     /locus_tag="PLO59_10305"
     CDS             complement(931..>1183)
                     /locus_tag="PLO59_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HQV01542.1"
                     /translation="VIGLPRIKINTPLHSKFSGAIAHVEIEGYTAATLEAALFQQFKI
                     HTSPIGWAYLDGVRITPNVYTRLSELDVLVEAIKTLAKK"
BASE COUNT          405 a          249 c          211 g          318 t
ORIGIN      
        1 ttacctaaca tagccgatag cagtaccacc tgtatgggat tggtacacat taccaatgct
       61 gcattaattg gttgcgttag cgacaaccct ttaaaaaaca aaagttgatt tattgccact
      121 ccgaacaaag cacattgcat taataaaaac aggtgtttac gggcaacttt ttgcttaata
      181 aaaaacaaat gaataatcag aaacaatatg gctgccgaca ccactctaat caatataaaa
      241 gcaaacgggc ctataaatgc cggcattaca tcttttgcaa tcgaaaaatt tatcccataa
      301 ataatattag ctgttaatac agctaaatgt gcaagcacaa ttttacgcac ggctgttact
      361 gtttatttgc tacctgcaaa gcaataaatt aattaagttc acatagctaa ccgaacattt
      421 ttaaactgta accattatgc attgataaaa actgcatatt cgtgcaacga aaaaattaat
      481 ttatcaaaca catggcatta gacattaccg gaaaaattat tcacatactg cccgaagccg
      541 gtgggcaaac caaggcaggc aaaccttgga gtaaacaaga gtttgttatc gaaaccgaag
      601 aacaatatcc taaaaaagta tgcattagtt tgatgggaga aaaagtacag gagttgaaaa
      661 aatttagcat cggacagcag gttaaagcat cacttaacat cgaatcgcgc gagtataatg
      721 gacgctggta tagtgatata cgtgcctggc gtttagaagc cgctggcagc acccaacccc
      781 aaagtaatta tgcaccgcct gccaacacac ctgctgactc atttagcagc aacccattgc
      841 cggcagcaac cgatgatgat ttaccatttt aaaacattgc atatctaaaa aaagccgctc
      901 ttgcagagcg gcttttttat ttgttcgaat ttatttttta gctaatgttt ttattgcctc
      961 aaccaaaaca tccaattcgc ttaaacgggt ataaacatta ggcgtaatac gtacgccatc
     1021 caaatacgcc cagccaattg gcgaagtatg aattttaaac tgctgaaaca atgctgcctc
     1081 taaagttgct gccgtgtaac cctcaatttc aacgtgtgca atggcgcccg aaaattttga
     1141 atgtagcggt gtgtttattt ttatacgcgg caagccgata acc
//
LOCUS       DAOMAA010000862         1182 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_157153_length_1182_cov_2.730257, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000862 DAOMAA010000000
VERSION     DAOMAA010000862.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157153_length_1182_cov_2.730257"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..957)
                     /locus_tag="PLO59_10310"
     CDS             complement(<1..957)
                     /locus_tag="PLO59_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HQV01543.1"
                     /translation="MPVHYISSQHLDFDTIKNIINQQTRLALSDDAIKRIEHCRSYLD
                     KKMQQQTDAIYGINTGFGSLYNKSISNDQLEQLQANLVMSHACGCGTEVPQEIVKLML
                     FLKIQGLSYGNSGVQLITVQRLIDFFNNDVLPVIYTQGSLGASGDLAPLAHLSLPLLG
                     LGQVYYKGIKQHTNLVYKALGWQAIQLKSKEGLALLNGTQFMSSYATWCLIKAEEITG
                     LSDKIAALSLDAFDGRIEPFNHFIQQVRPHKGQIETAITFRQLLKDSEIISQKKQHVQ
                     DPYSFRCIPQVHGASKDAINHVAHVVFTEINSVTDNPTIFPDD"
BASE COUNT          345 a          206 c          218 g          413 t
ORIGIN      
        1 atcatccgga aaaatggttg ggttatcggt aaccgaatta atttcggtaa ataccacatg
       61 tgccacatgg ttaatagcat ccttactggc accatgtact tgtggtatgc aacggaaaga
      121 gtaggggtct tgcacatgct gtttcttttg tgaaataatt tctgaatctt ttaaaagttg
      181 gcggaatgtg attgccgttt ctatttgtcc tttatgcggc ctaacttgtt gtatgaaatg
      241 attaaaaggt tcgattcgtc catcaaaagc atctaaactt aaagcagcaa ttttatcgga
      301 tagacccgtt atttcttcgg ctttaattaa acaccaggtg gcataactgc tcataaactg
      361 cgtgccgttt aataaagcca aaccttcttt tgatttaagt tgaatggctt gccaacctaa
      421 cgctttatat acaaggtttg tatgttgctt aatgcctttg taatacacct gacccaaacc
      481 taacaagggt aagcttaaat gcgccaatgg tgccaaatcg cccgatgcac ctaatgagcc
      541 ttgtgtataa attaccggca aaacatcgtt gttaaaaaaa tcaatcaatc gttgtactgt
      601 aattaattgc acgcctgaat tgccataact taagccctgt atttttaaaa acaacatcag
      661 ctttactatt tcttgaggta cttcagtgcc acagccgcaa gcatggctca taaccaaatt
      721 ggcttgtaat tgttctaatt gatcgttgct gattgattta ttataaagtg agccaaagcc
      781 cgtattgatg ccgtaaatag catcggtttg ttgctgcatt tttttgtcta agtaactgcg
      841 gcaatgctca atacgtttaa tagcatcatc acttaatgcc aaacgtgttt gttggttgat
      901 gatattttta atggtatcaa aatctaaatg ttgcgaggag atgtaatgga ctggcatttg
      961 gtttatatta ttaatcagca aattaatttt attaaaaatt caatttttta atgcgtatgg
     1021 gatctttaaa ttttaattgg atttataagc gtatatcaac ttagttgttg taccaatgtt
     1081 tcgaaatcaa ctttatcctg catgcctgtt gtcatgtttt ttaaagtgat ggtttgagat
     1141 ttaatttctt catcgcctat tatggcaaca aaaggaattt tt
//
LOCUS       DAOMAA010000863         1182 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_157163_length_1182_cov_2.664596, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000863 DAOMAA010000000
VERSION     DAOMAA010000863.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157163_length_1182_cov_2.664596"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /locus_tag="PLO59_10315"
     CDS             complement(<1..817)
                     /locus_tag="PLO59_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018478905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HQV01544.1"
                     /translation="MRSYIYMLSVLIFSNWLSGTLVQCQSLDTVQLNAMQYRCIGPYR
                     GGRSCAVVGVNDQRNVFYMGATGGGVFKTTDGGSNWKNVSDGYFGGSIGAIAISNTDK
                     DVIYVGQGEQTVRGNVSEGFGMWKSTDAGRTWLACGLTDTRHINKIVIHPKNADVVYV
                     AALGHLFGKNTERGIFKTTDGGKSWKKILYVNDSVGASDICMDPNNPMILYAGMWHVK
                     RTPYSFESGGPGSGLYKSIDGGETWKNISGSDGLPTDTLGIVMIAVSPVNSNRV"
BASE COUNT          371 a          259 c          183 g          369 t
ORIGIN      
        1 aaaccctgtt actgtttacg ggcgaaacag caatcatcac aatacctaat gtatcggtgg
       61 gcaatccatc actaccggaa atgtttttcc aggtttcgcc accatctata cttttataca
      121 atcctgaacc gggcccacca ctttcgaaac tatatggtgt acgttttaca tgccacatgc
      181 ctgcatataa aatcatcgga ttgttgggat ccatacaaat atcgcttgcg cccacactat
      241 catttacgta aagaattttt ttccaacttt tgccaccatc tgttgtttta aaaataccgc
      301 gttctgtatt cttgccaaat aaatgaccca atgcagcaac ataaacaaca tcagcatttt
      361 tgggatgaat tacaatttta ttgatatggc gcgtatccgt taatccacac gccaaccagg
      421 ttcggccggc atcagtgctt ttccacatac caaagccctc gctaacattt cctcttactg
      481 tttgctcgcc ttgcccaaca taaattacat ctttatccgt attactaatt gctattgcgc
      541 caatgcttcc tccgaaataa ccatcagaca cgtttttcca attactgccg ccatcagttg
      601 ttttaaaaac cccaccaccg gttgcgccca tataaaacac attacgttgg tcgtttacac
      661 caacaacagc acaactgcgg ccacctctgt atgggccaat gcagcggtat tgcatggcat
      721 taagttgcac agtatctaaa ctttgacatt gaaccaaagt gcctgaaagc caatttgaaa
      781 aaattaaaac cgataacatg taaatgtaac ttctcatttt attaaatata taactgacca
      841 attaagcgaa ttataataca ctttaactca tacatagatt attaacattg ttttattaag
      901 tagtttctaa atcatttggg gtatcacaag tataaaatta gcattggtta gtgtagcatt
      961 atattttatg cattgcttta gtaatggaag tgattacacg taactaataa taagagataa
     1021 atgggatgtt ttgtttgtgt tacgaattaa cattatgata atataacagc aagatgcgtt
     1081 aactttatag taatgctgcg ataacacttt tgccccataa aaattaatca atgcgggcaa
     1141 tattagcagc tttactgttt ttaagtataa cagcaacggc ac
//
LOCUS       DAOMAA010000864         1181 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_157412_length_1181_cov_2.820604, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000864 DAOMAA010000000
VERSION     DAOMAA010000864.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157412_length_1181_cov_2.820604"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1181
                     /locus_tag="PLO59_10320"
     CDS             <1..>1181
                     /locus_tag="PLO59_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018413937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HQV01545.1"
                     /translation="TFGTGAATNSIIDLEYTDCIMVIGANPTDAHPVTGAKIKQAALK
                     GKTLIIIDPRKTELVKYAQYHLQLKPGTNVALLNMMLFYIIKEQKEDKDFIASRTEGY
                     DAFKQQMLNLNMDEMEAITGVPKELVRDAAIAYASANNAMSFHGLGVTEHSQGTFTVM
                     QIADLAMITGNIGRRGVGVNPLRGQNNVQGAADMGCQPHQGAGYLDVTLPEYHKKYES
                     FYGVNLPQHAGLKIPQMYDAALNQKFKALWVIGEDMAQTDPNTQHVVKAIEQLDLFIV
                     QELFMTETSKLAHVILPGASFLEKSGTFTNGERRIQRVNAVVDPLPGTKTDGRIITDI
                     MNKMGYKQADFDAPTLLNEIAQIVPFFAGVTWDNLGENGKQWPVLPDGSDTEILHTDT
                     F"
BASE COUNT          388 a          230 c          253 g          310 t
ORIGIN      
        1 gtacatttgg taccggtgcg gcaaccaaca gcataattga tttagaatat accgactgta
       61 taatggttat tggagccaat ccaaccgatg cacatccggt aaccggtgca aaaattaaac
      121 aagcggcatt aaaaggcaaa acgcttataa taattgatcc gcgtaaaacc gagttggtta
      181 agtatgcaca atatcacctg caacttaagc ccggcaccaa tgtggcttta ttaaacatga
      241 tgttgtttta cattattaaa gagcaaaaag aagataaaga ttttattgca agccgtaccg
      301 aaggctatga tgcatttaaa caacaaatgc ttaacctgaa tatggatgaa atggaagcga
      361 taaccggtgt acctaaagaa ttagttagag atgcagctat agcgtatgca agtgcaaaca
      421 atgccatgtc gtttcacgga ttgggcgtta ccgaacattc gcaaggtact tttaccgtaa
      481 tgcaaattgc cgacctggcc atgataaccg gaaatattgg gaggcgtggt gttggcgtaa
      541 atccgttgcg tggccaaaat aatgttcagg gagcagccga tatgggatgc cagccgcatc
      601 aaggtgcagg ttatttagat gtaacacttc ctgaatatca taaaaaatat gaatcctttt
      661 acggtgttaa tttacctcaa catgccgggc ttaaaatacc gcagatgtat gatgcagctt
      721 taaatcaaaa atttaaagcc ctatgggtaa ttggcgagga tatggcccaa accgacccta
      781 atacgcagca tgtggtaaaa gctattgaac aattagattt atttattgtg caagaacttt
      841 ttatgacaga aacatctaag cttgcccatg ttattttacc gggcgcatcc tttctcgaaa
      901 aaagtggcac atttaccaat ggcgagcgca gaatacaacg cgtaaatgca gtagttgatc
      961 cattacccgg taccaaaacc gatggcagaa ttataactga catcatgaat aaaatgggct
     1021 acaaacaagc cgattttgat gcacctaccc tactgaatga aatagcacaa atagttccgt
     1081 tttttgccgg agttacctgg gataatttag gcgaaaatgg taaacaatgg cctgtattgc
     1141 ccgatggcag cgatactgag attttgcata ccgatacatt t
//
LOCUS       DAOMAA010000865         1180 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_157688_length_1180_cov_3.132444, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000865 DAOMAA010000000
VERSION     DAOMAA010000865.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157688_length_1180_cov_3.132444"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="PLO59_10325"
     CDS             <1..502
                     /locus_tag="PLO59_10325"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007548199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HQV01546.1"
                     /translation="ILNGVNIGENCNICDHCFIESQVSIGNNVTIKSGIYIWAGTTIE
                     DDVFLGPNVVFTNDIRPRSKQFLQNPVPTLVQKGASIGANSTILAGITIGKYAMCGIG
                     SVVTRDVKNYALVFGNPARQQGWVNERGEKLQQRTASLWESTNGDIYKETSEGLKPHK
                     KQKNKF"
     gene            499..>1180
                     /locus_tag="PLO59_10330"
     CDS             499..>1180
                     /locus_tag="PLO59_10330"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQV01547.1"
                     /translation="MTTLSVIVPFYFNQANVQVTTDTLFENEKLFEPDLKFQYIFIDD
                     GSKDDTLQLLKQVKQNSSRQIVIIKLAGNFGSYNAILAGMQYATGDCNVVITSDLQDP
                     PPLILKMYNYWQQGFKLVLANRNKRNDSFITNFFANTYHKLIKKFASINLPDGGFDFC
                     LFDKQLRQQVVDMDEKNTNSLYLLVWLKYDYISIAYTRQERKLGKSRWTLNKKIKLFI
                     DSFVSFSYL"
BASE COUNT          396 a          192 c          227 g          365 t
ORIGIN      
        1 tattttaaat ggcgttaaca ttggtgaaaa ttgtaacatt tgcgaccatt gttttatcga
       61 gtcgcaagta agcatcggaa ataatgttac cataaaaagt ggtatttata tttgggccgg
      121 tactactatt gaggatgatg tgtttttagg tccaaacgta gtttttacca acgatataag
      181 gccacgcagt aaacagtttt tgcaaaaccc tgttccaaca ctcgtacaaa aaggagcttc
      241 aattggtgca aactcaacca tattagcagg cattacaata ggtaagtatg ccatgtgtgg
      301 tattggtagt gtagttacac gcgatgttaa gaattatgct ttagtatttg gtaaccctgc
      361 caggcaacaa ggttgggtaa acgaacgtgg cgaaaaatta caacaacgta cggcatcgtt
      421 atgggaaagt actaatggtg acatttataa ggaaacttca gaaggtttaa aaccacataa
      481 aaaacaaaaa aataaatttt gaccacgctt tcagttattg taccatttta ttttaatcag
      541 gctaatgtgc aggttactac ggatacattg ttcgagaacg aaaaactatt tgagcccgat
      601 ttaaagtttc aatatatttt tattgatgat ggaagcaaag atgatacgct tcaactctta
      661 aaacaagtga agcaaaatag tagccggcaa atagtaatta taaaacttgc aggcaacttt
      721 ggttcgtata atgccatact tgccgggatg caatatgcaa ccggtgattg taacgtggtt
      781 attacttccg acctgcagga cccgccacca ttaattttaa aaatgtataa ctactggcaa
      841 caagggttta aattggtttt ggctaaccgt aacaaacgaa acgattcatt tattaccaat
      901 ttttttgcta acacttatca taagctcatt aaaaaatttg cttcaattaa tttgccagat
      961 ggtggatttg atttttgctt atttgataaa caactacgtc aacaagtagt tgatatggat
     1021 gagaaaaaca ccaactcatt gtatttatta gtttggctta aatacgatta cataagcatt
     1081 gcctacacaa ggcaagagcg taagctgggc aaaagccgct ggacgttaaa taaaaaaatt
     1141 aagcttttta ttgattcgtt tgtgtcgttc agttatttgc
//
LOCUS       DAOMAA010000866         1180 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_157702_length_1180_cov_2.816889, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000866 DAOMAA010000000
VERSION     DAOMAA010000866.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157702_length_1180_cov_2.816889"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1180)
                     /locus_tag="PLO59_10335"
     CDS             complement(<1..>1180)
                     /locus_tag="PLO59_10335"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HQV01548.1"
                     /translation="NTPIALDCYYQALKLHEEAGDLAGVSTVLNNIGYLKNSQHDKAS
                     ALQHFKRSLQIQYQINDSAGMALSLLNIGSIYDDALKADSALTNYQQSVLISKAISNQ
                     QTEAIALSNMGIYFDKNQMHDSAKFYYTQAFNIQQQIQDVEGIATSQINFGNLYLNEK
                     KYPLAQQHFEYAFSLANQTGYIKLKRGAALWLSKVYALQHNYKDAYQMHVLHKQLADS
                     VSNAETRKAAVKKQMQYEYDKKTAVATALHEKQTLIAKNEIEKQHLIKKIILIAAILL
                     LLILGFGFYNFYTRRKETFLKTIAQVNNQALRAQMNPHFIFNSLNSIQDFINHHDIPN
                     ANTYIVKFAKLVRLILENSRHHEVLLSDDLNALEWYMQLENLRLPHGFDYEINVDKNI
                     D"
BASE COUNT          313 a          199 c          238 g          430 t
ORIGIN      
        1 tgtcaatgtt tttgtcaacg ttaatttcat aatcaaaacc gtgcggtagc cgcaagtttt
       61 ctaattgcat ataccattct aaggcattca aatcatcaga aagtaatact tcatgatgcc
      121 tgctgttttc aagtattaag cgcaccagct ttgcaaactt tactatgtaa gtattggcat
      181 taggtatatc gtggtggtta ataaaatcct gtatggagtt aagcgagtta aaaataaagt
      241 gcggattcat ttgcgcacgc aatgcctgat tgttaacctg tgcaatggtt tttaaaaatg
      301 tttcttttct gcgtgtataa aaattataaa aaccaaaccc cagtatcagc aataagagta
      361 tagcggcaat gagtatgatt ttttttatca gatgttgttt ttctatttcg ttttttgcaa
      421 tcaatgtttg tttttcgtgc agggcagtgg caacggccgt ttttttatca tattcatatt
      481 gcatttgttt ttttacggca gccttgcggg tttcggcatt acttacacta tcggcaagtt
      541 gtttgtgcaa aacatgcatt tggtatgcat ctttatagtt gtgttgcaaa gcataaactt
      601 tgctgagcca taatgcagcg ccccgtttta atttaatata accggtttga ttagctaaag
      661 aaaatgcgta ttcaaaatgt tgttgcgcaa gcggatattt tttttcgttt aaatagagat
      721 tgccaaaatt tatttgcgag gtggcaatgc cttctacatc ctgtatttgt tgttggatgt
      781 taaatgcctg cgtgtagtaa aattttgcac tgtcgtgcat ttgattttta tcaaagtaaa
      841 tacccatatt gctaagggct atagcttctg tttgttggtt gcttattgct ttacttatga
      901 gtacactttg ttgatagttc gttaaggcgc tatccgcttt taatgcatcg tcataaatac
      961 tacctatgtt aagcagcgat aaagccatgc cggcactgtc gtttatttga tattgaattt
     1021 gaaggctgcg cttaaagtgc tgcaaggcac ttgctttatc gtgctggctg ttttttaaat
     1081 agccaatatt attgagcacg gttgaaacac ctgctaaatc accggcctct tcatggagct
     1141 ttaatgcctg atagtaacaa tcgagtgcaa tgggtgtgtt
//
LOCUS       DAOMAA010000867         1180 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_157708_length_1180_cov_2.764444, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000867 DAOMAA010000000
VERSION     DAOMAA010000867.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157708_length_1180_cov_2.764444"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1180
                     /locus_tag="PLO59_10340"
     CDS             <1..>1180
                     /locus_tag="PLO59_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQV01549.1"
                     /translation="NPADPNYLYVASEIGGFNMGLNFTYFFGQDELKYGLEVLGFKTD
                     FEFYNAAGNRLGQEENTTEFGTYLNYRKVAGKFVIQPGLRLHYYSSLTELAIEPRFSA
                     KYNATDFLRFKLAAGIYKQNFLSATSDRDVVNFFYGFLSAPENLPANFNGSKVDSRLQ
                     QANHFILGTEIDLPHHFSVNIEGYIKDFAQVINVNRNKIYDDVGANSDKPDELKKDFI
                     VENGIAKGIDFNLKYEYKKVYLWFVYSIGFVDRFDGNDEYMPSFDRRHNVNIVASYTF
                     GKDLLWELNSRLNYGSGFPFTPTQGFYPYQNFSQGVNTNYNSSNGQLGIIYGELNANR
                     LPDYARLDLTLKRTFVLSSRSNLEATASVINLLNRQNIFYFNRVTFQRVDQLPILPAL
                     G"
BASE COUNT          354 a          203 c          232 g          391 t
ORIGIN      
        1 caacccggcc gaccctaatt atttatatgt ggccagcgaa attggtggct ttaatatggg
       61 gttaaatttt acctactttt ttgggcagga tgaattgaag tacggtttag aggtattggg
      121 atttaaaacc gatttcgaat tttataatgc agccggcaat cgtttagggc aagaagaaaa
      181 cactacagag tttggcactt atttaaacta ccggaaagtt gcaggcaagt ttgtaattca
      241 gccaggtttg cgtttacact attactcttc attaaccgag ttggctatag agccacgctt
      301 tagtgctaaa tataacgcta ctgatttttt acgctttaaa ttggctgccg gtatttacaa
      361 gcaaaacttt ttaagtgcca caagcgatag agatgtagta aatttctttt atggattttt
      421 atcggctccc gaaaacttac ccgctaattt taacggcagt aaggttgact cgcgtttgca
      481 acaagcaaac cattttattt taggtactga gattgattta ccacatcatt tttcggttaa
      541 tattgaagga tatattaaag attttgctca ggtaataaat gtaaaccgca ataaaattta
      601 tgatgatgtt ggcgctaata gcgataaacc ggatgagcta aaaaaggatt ttattgttga
      661 aaatggaatt gccaaaggca ttgactttaa tttaaaatat gaatacaaaa aagtatatct
      721 gtggtttgtt tactctatcg gttttgttga taggtttgat ggtaacgatg agtatatgcc
      781 cagttttgac agacgccaca atgtaaatat tgttgccagt tacacctttg gtaaagattt
      841 actttgggag cttaactcgc gccttaatta cggttcgggg tttcctttta cacctacgca
      901 aggtttttat ccttaccaaa atttttcgca aggtgttaat acaaattaca acagcagtaa
      961 tggccagtta ggtattattt atggtgagtt aaatgccaac cgtttacccg attatgcacg
     1021 cttagaccta acacttaaac gcacctttgt attaagcagt agaagcaatt tagaggctac
     1081 tgccagtgta attaacttat taaaccggca aaatattttt tacttcaacc gtgttacctt
     1141 tcaacgcgtt gatcagctcc ctatattacc ggcattggga
//
LOCUS       DAOMAA010000868         1179 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_157955_length_1179_cov_2.982206, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000868 DAOMAA010000000
VERSION     DAOMAA010000868.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157955_length_1179_cov_2.982206"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            372..>1179
                     /locus_tag="PLO59_10345"
     CDS             372..>1179
                     /locus_tag="PLO59_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019947267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HQV01550.1"
                     /translation="MKKLLVWCMFYIGVLNVYAEEGMWLPIMLKQMEAQMQNRGLKIS
                     AEAIYDVNNTSLKDAVVLFGGGCTGEIISKQGLLLTNHHCGFGAIAGLSTIENDYLKN
                     GYWAYNQTQELPAPGLTATFIIRIEDVTAQIAPYLTQGMTEAERNSKIKSLAAAIESK
                     AIAGTHYEASVKPFYSGNDFILFVSETFKDLRLVGAPPNAIGNFGEETDNWIWPRHTG
                     DFSLFRIYAGPDNKPAAYNKNNVPFLPRKHLQISTAGITENDFTMVYGFPG"
BASE COUNT          348 a          205 c          247 g          379 t
ORIGIN      
        1 ccgcgttgta ccaatagttt gcacaacgat gggtgtattt ttaattgttg ctgcaatgca
       61 tccacttgtg cttgttggta attctgattc aggttccagc gcttgtgcat gtattggttg
      121 tgatattatg gtggtgtaca aacctacatg ttatatctac tattatcaat ttatttttta
      181 gggtcttata tgtttgttca aattagcaga tggtaaaacg taatgccaat taaattgaag
      241 tttaacgata tttagtaagt aggtttttgc atttggttac attcgccagc gttattgaag
      301 tttaacgata tttagtaagt aggtttttgc atttggttac attcgccagc gttacttaat
      361 ttttatttgc tatgaagaaa ctacttgtat ggtgcatgtt ttatataggc gttttaaatg
      421 tttatgccga agaaggcatg tggctgccta ttatgttaaa acagatggaa gcgcaaatgc
      481 aaaaccgcgg acttaaaata agtgccgaag ccatttatga tgtaaacaac acatcgttaa
      541 aagatgctgt agttttattt ggaggcggat gtacaggcga aattatttcg aaacaaggtt
      601 tactgcttac taaccatcac tgtggttttg gtgccattgc cggattaagt accattgaaa
      661 atgattattt aaaaaacggg tactgggcct ataatcaaac acaagagttg ccggcaccgg
      721 gtcttacagc cacttttata atcaggattg aagatgttac cgcacaaatt gcaccttacc
      781 ttacccaagg tatgacagag gccgaacgta acagcaaaat aaaaagttta gctgcagcaa
      841 ttgaaagtaa agctattgcc ggtacacact acgaagcatc ggttaagcct ttttacagtg
      901 gtaatgattt tatattattt gtaagcgaaa catttaaaga tttgcgtttg gtaggcgcac
      961 ccccaaatgc cattggcaac tttggcgaag aaaccgataa ctggatttgg ccacgccata
     1021 ccggtgattt tagtttgttc agaatttatg ccggccccga taataaaccc gctgcttaca
     1081 ataagaataa tgtaccattt ttgccgcgca aacacttaca aattagtaca gccggtataa
     1141 ctgaaaacga ctttacaatg gtgtacggtt ttccgggtc
//
LOCUS       DAOMAA010000869         1177 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_158562_length_1177_cov_2.010695, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000869 DAOMAA010000000
VERSION     DAOMAA010000869.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158562_length_1177_cov_2.010695"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="PLO59_10350"
     CDS             complement(<1..232)
                     /locus_tag="PLO59_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020606818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HQV01551.1"
                     /translation="MKRILIGLLFLTIASTTHAQDPQFSQFYAAPLYLNPALAGSAHC
                     PRVTLNYRNQWPALNNAFVTYAAGYDQNIDALS"
     gene            468..>1177
                     /locus_tag="PLO59_10355"
     CDS             468..>1177
                     /locus_tag="PLO59_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01552.1"
                     /translation="MFRNYLVLTVFIFCGFISGKSQTISRTLIWEDYKTYHFSKTVSA
                     NILYFENCAPDVSKNNMPFYSERFELASSQPLNFELSNAVYADLVVPAGVTMWDSIGF
                     DIKPQTTITTYKKQSAAWVSFLPIRKNQTTGRDEKLISFTLTSVVSGLKGSANTMAHR
                     VYAAESVLASGKWYKIGVTNDGVYQLNYNFLKNTLGIADIDKLNPQNLRIYGNGGGMV
                     PMLNSIWRPDDLLENAILV"
BASE COUNT          362 a          201 c          223 g          391 t
ORIGIN      
        1 ccgataatgc atcaatattt tgatcgtaac cggcagcata ggtaacaaag gcattgttta
       61 aagcaggcca ttggtttctg tagtttaatg taacccttgg gcaatgtgcc gagccggcta
      121 atgccgggtt caaataaagc ggggcagcat aaaactgtga aaattgggga tcttgtgcat
      181 gggttgttga tgcaatagtt aaaaacaaca atccgattaa aattcttttc atagatagat
      241 ttacagcttg cacaaatggc caaccaaacg cgtttattgt gcaataataa ttatttgttt
      301 ttgtcgtttc cgatttcgtg tatttttttt ttgcgcccat aaaactactt attattcgaa
      361 tttcgtaaac ataagcagtg attattgact tagaagttac aatttacaat ggttttagta
      421 atacttttga atctcttaca ttaatatcta tcaatactca attttacatg tttcgtaatt
      481 atctggtttt aactgtattt atcttttgtg gttttataag tggaaagtcg caaacaataa
      541 gtcgcacctt aatttgggaa gactataaaa cgtaccattt ttcgaaaacc gtatcggcta
      601 atattttgta cttcgaaaat tgtgcaccgg atgtttcgaa aaacaatatg cctttttatt
      661 ccgaaagatt tgaattggca tcaagccaac ctttaaactt tgaattatcg aatgctgttt
      721 atgctgattt ggtggtcccc gctggtgtta ccatgtggga tagtattgga tttgatatca
      781 aaccacaaac aaccattaca acttacaaga agcaaagtgc agcctgggtt tcgtttttac
      841 ctatacgaaa aaaccaaact acaggtcgcg atgaaaaact aatctccttt actttaactt
      901 cagttgtttc gggcttaaaa ggtagtgcaa atacaatggc gcatcgggtt tatgcagccg
      961 aatcggtttt agcatcgggc aaatggtata aaataggtgt tacaaatgat ggtgtttatc
     1021 aactcaacta taatttttta aaaaacacct tgggaattgc cgacattgac aaactcaatc
     1081 ctcaaaattt gcgcatatac ggcaatgggg gtggtatggt acccatgtta aacagtattt
     1141 ggcgacctga tgatttactt gaaaatgcca ttttagt
//
LOCUS       DAOMAA010000870         1176 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_158692_length_1176_cov_3.120428, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000870 DAOMAA010000000
VERSION     DAOMAA010000870.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158692_length_1176_cov_3.120428"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="PLO59_10360"
     CDS             complement(<1..461)
                     /locus_tag="PLO59_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01553.1"
                     /translation="MEKRLQWSQQIFYFSIIALVIGMPLSIFLISLSQFGLAVSWLLA
                     GNYKQRLAGYFTGTVGLVFTMFFVMHLVGLSYTTDFKYAFNDIRIKLPLLLLPFFMAT
                     MPPLSKTKWQSVLSFFVAACLMGTFICTYIWAGFGTKVITDARQISIFISH"
     gene            complement(526..>1176)
                     /locus_tag="PLO59_10365"
     CDS             complement(526..>1176)
                     /locus_tag="PLO59_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HQV01554.1"
                     /translation="KYYIPSGRCIQALDYSNRNADGSVGHVPDSLMTPFKTKLGRTVY
                     DGGGVLPDFTTTVDTASSIAVSLASKFLVFDYATTYRNTHNTIDNAKAFKLNDETYNS
                     FVSWLKDKDYDYTTRSEKIIEDLKQASIRDKNYDDAKAEFEALKTKLSHSKTSDLVKH
                     KQEISEMLENEIVSRYYYQKGRAENEFNYDAEVKKALAALADNNLYNTLIKTNHTK"
BASE COUNT          377 a          223 c          207 g          369 t
ORIGIN      
        1 atgtgcgaaa taaatatcga aatctggcgt gcatcggtaa taactttagt accaaagcca
       61 gcccatatat atgtgcatat gaatgtaccc attaaacatg cggcaacaaa aaagctaagt
      121 acgctttgcc attttgtttt tgacagtggt ggcattgtag ccataaaaaa aggtaagagt
      181 aaaagtggca gcttaatcct tatatcatta aaagcatatt taaaatcggt agtataactt
      241 aagccaacca ggtgcatcac aaaaaacata gtaaaaacca aaccaaccgt accggtaaaa
      301 tagccggcta atctttgctt ataatttcct gccagcagcc aacttacagc caaaccaaat
      361 tgcgaaaggc tgatcaggaa aatagaaagt ggcatgccta taaccaaggc aattatggag
      421 aaataaaata tctgttgcga ccactgtaat cgtttttcca tacacaaata aaaaagcagc
      481 taataaagta ttaactgctt ttttaaaaaa ttattattac caacactact tggtatgatt
      541 ggtctttatt aaggtattat acaaattgtt atcggccaat gccgctaatg cttttttaac
      601 ttctgcatca tagttaaact cattttcggc acgtcctttc tgatagtaat aacgtgatac
      661 aatttcgttt tccaacattt cggaaatttc ttgtttatgc ttaactaaat cagaagtttt
      721 gctatgactt aatttggttt taagcgcttc gaattcagct ttggcatcat cgtaattttt
      781 atcgcgtatg gatgcttgct ttaagtcttc aataattttt tcgctgcgtg tggtatagtc
      841 ataatctttg tcctttaacc agctaacaaa actattatat gtttcatcgt ttaatttaaa
      901 ggcctttgca ttatcaatcg tgttatgcgt attacgatac gttgttgcat aatcaaacac
      961 taaaaattta gatgccaaac taacggcaat actgctggct gtatcaacag tagtagtaaa
     1021 gtcgggcaat acgccaccac catcatatac cgtacgtcct aatttggttt taaaaggagt
     1081 cattaacgag tcgggcacgt ggccaacgct gccatcggca ttgcggttgc tgtaatcaag
     1141 tgcctggatg catcgtccgc tgggaatata gtattt
//
LOCUS       DAOMAA010000871         1176 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_158697_length_1176_cov_3.028546, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000871 DAOMAA010000000
VERSION     DAOMAA010000871.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158697_length_1176_cov_3.028546"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PLO59_10370"
     CDS             complement(<1..182)
                     /locus_tag="PLO59_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01555.1"
                     /translation="MLQFTLNWIAYGNPKQVFNALTQVDAIEKWSGQKAVFDTKLNTY
                     QWFDWVSGTIEIYEPNT"
     gene            complement(267..623)
                     /locus_tag="PLO59_10375"
     CDS             complement(267..623)
                     /locus_tag="PLO59_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013750791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HQV01556.1"
                     /translation="MKKIYHSSTCSTNQRILSEIKPKKDVVLQDIKTEPITPAQIDEM
                     KKLAGSYEALFSRVALKYRSMGLNQMQLTEKDYRKYILQEYTFLKRPVIIVNDAIFVG
                     NTAKVVAAAKTALSQK"
     gene            798..>1176
                     /locus_tag="PLO59_10380"
     CDS             798..>1176
                     /locus_tag="PLO59_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008509095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HQV01557.1"
                     /translation="MTTDNYFEIKLPVFEGPFDLLLFFIERDELDIHNIPIAKIANDF
                     LDYMHHLESMNMELASEFILVASTLMRIKAKMLLPRYKKDEMGNDIDPRQDLIQQLLD
                     YKRYKEATETFKQKEEQRMMQTER"
BASE COUNT          371 a          203 c          213 g          389 t
ORIGIN      
        1 gtgtttggtt catatatttc aatagtaccg cttacccaat caaaccattg ataggtgttt
       61 aattttgtat cgaacacagc cttttggcca ctccattttt cgatagcatc aacttgtgtg
      121 agggcgttaa aaacctgttt tggatttcca taagcaatcc agttaagtgt aaattgtaac
      181 atgccgcttt ttattatttg ttgccaaagg tattacatat cgtacatagc aaacactata
      241 ttaatctaat acaaatgctg ccatgcctac ttttgtgata aagcagtttt tgcagcagct
      301 acaactttgg ctgtgttgcc tacaaaaatg gcatcgttta ctataataac cggacgtttt
      361 aaaaaagtat attcctgtaa aatgtatttg cgataatctt tttcggtaag ttgcatttga
      421 tttaaaccca tgctgcgata ctttaaagca acccggctga aaagtgcttc ataactaccg
      481 gccaattttt tcatttcatc aatttgtgca ggtgttatgg gttcggtttt tatgtcttgc
      541 aaaaccacat cttttttagg tttaatttct gacaagatgc gttggttggt actgcaagtg
      601 ctggaatgat aaattttttt cataaatttt ttaagcggtt tttaaatcca agtgataata
      661 aaagtgctgc aaattaaagc tacaagtgtt attcacaaaa caggctactt accaacatgg
      721 ataaggctaa gcacaatttt tatttgttta tttggatgca ggccaaagca gctttgggct
      781 aagtaaaatt ttaaaaaatg acgactgata attatttcga aataaagctt ccggtttttg
      841 aaggcccttt tgatttacta ttgtttttta ttgagcgcga tgagttagat atacacaaca
      901 tacccattgc caaaatagcc aacgattttt tagactacat gcatcatctc gaaagcatga
      961 acatggagct tgccagcgag tttattttag ttgccagcac cttaatgcgg ataaaagcta
     1021 aaatgctttt gcctcgttat aaaaaagatg aaatgggcaa tgatattgac ccgcgccaag
     1081 atttaattca gcaattgtta gattacaaac gatacaaaga agctacagaa acgtttaagc
     1141 aaaaagaaga gcaacgcatg atgcaaacag agcgtg
//
LOCUS       DAOMAA010000872         1175 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_158967_length_1175_cov_2.792857, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000872 DAOMAA010000000
VERSION     DAOMAA010000872.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158967_length_1175_cov_2.792857"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            295..861
                     /locus_tag="PLO59_10385"
     CDS             295..861
                     /locus_tag="PLO59_10385"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HQV01558.1"
                     /translation="MLPAFKRKPQTGNILIAEPFLHDKSFKRSVIWLTNHDENGSRGF
                     ILNKQTNIKLNKLIDDFPMFDIPVFYGGPVETDSLFFIHTAGKLIEGAEHFLPGYYVG
                     GNFDTLKFLIDTKQLKTYQVKFFIGYSGWVANQLESEMNELTWMVTDFKNELGINADN
                     ETLWSNYVKGMGKDYTLVANSPEYPSLN"
     gene            877..>1175
                     /locus_tag="PLO59_10390"
     CDS             877..>1175
                     /locus_tag="PLO59_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008631623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HQV01559.1"
                     /translation="MIETCTLLNFISNGHTIIDVRSENEYLQGHIPGAINLPILNNEV
                     RKAVGTCYKQNGQEAAVTLGFELVGPKFSEFIKEAQKIAHEKELNIYCWRGGMRS"
BASE COUNT          399 a          194 c          214 g          368 t
ORIGIN      
        1 tacataaatt catagcataa ggctcgtcat aatttttggc tacagctaac tcaaaccacg
       61 ttttaaactg ttcgaatggc gagtggtgca tcatatcctc atgtaagtcg ctcttattat
      121 aatcgcgtct gctgttgtaa ataaaatcat ctaaggccat tatatttttt ttttaaagat
      181 aggtagttcg caaaatgcaa aatagtattc attaaaatct ttattaaaag gtttttacaa
      241 attgatgttt ttaataatag taaaacattc taatatcgca tcaaatacaa aattatgtta
      301 cctgccttta aacgtaagcc gcaaaccgga aacattttga ttgcagagcc atttttacac
      361 gacaaaagtt ttaagcgttc ggtaatatgg cttaccaatc atgatgaaaa cggaagtcgt
      421 ggatttatac ttaataaaca aaccaatatc aaactcaata aattaattga cgattttcca
      481 atgtttgata tacctgtgtt ttatggaggc cctgttgaaa ccgattcgtt gttttttatc
      541 catacagccg gcaaattaat tgaaggtgcc gaacattttt tacccggcta ttacgtaggc
      601 ggaaattttg atacgcttaa atttttaatt gataccaagc aacttaaaac ctatcaagta
      661 aaatttttta ttggttactc gggatgggta gccaatcaat tagaatcgga aatgaatgag
      721 cttacatgga tggtaaccga ttttaaaaac gaattgggca taaacgctga taacgaaacg
      781 ctttggagca actacgtaaa aggcatgggt aaagattaca cattggtagc taactcgccc
      841 gagtatccca gcttaaatta acatgatggc ttttaaatga ttgaaacatg cacgctacta
      901 aatttcattt ctaacggaca taccattatt gatgttagaa gcgaaaacga gtatttgcaa
      961 ggtcatatac ccggtgctat taatttgccc attttaaata atgaggtccg aaaagcagtt
     1021 ggcacatgtt ataaacaaaa tgggcaagaa gcagcggtta cattaggttt tgaattagta
     1081 ggccccaagt tttcggaatt tattaaggag gctcaaaaaa ttgcacatga aaaagaacta
     1141 aacatttatt gttggcgtgg tggtatgcgt agcca
//
LOCUS       DAOMAA010000873         1174 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_159233_length_1174_cov_3.065237, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000873 DAOMAA010000000
VERSION     DAOMAA010000873.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159233_length_1174_cov_3.065237"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="PLO59_10395"
     CDS             <1..270
                     /locus_tag="PLO59_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01560.1"
                     /translation="KCYKAWVADDEGTKVEVWYTKDIVGNTTGTRQFKEIEGFMMRYE
                     VPVANMDGNMKMQCKSVQAQKVSDDMFVIPAGYEVKSQEDLMKGK"
     gene            386..>1174
                     /gene="chrA"
                     /locus_tag="PLO59_10400"
     CDS             386..>1174
                     /gene="chrA"
                     /locus_tag="PLO59_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HQV01561.1"
                     /translation="MDHTHNTPAYTLRQLVLYFLKLGYAGFGGPVALVGYMHSDLVEK
                     KGWISEEEYKEGLALAQLAPGPLAAQLGIYLGFVHYGILGATLCGLAFVIPSFIMVVL
                     IGIAYKIYGGLPWMQAVFYGVGAAVIGIIVMSSYKLTIKSLSKFSIVAIREKWLLWLF
                     FLVAGVITIITQSEDVLLFVFAGLLYMFIKAPPAWFSNQKTTQSIVLTGFSFLHFDSN
                     TLIDIAWFFTKAGAFVFGSGLAIVPFLHSGVVIKYQWLTEKQFVD"
BASE COUNT          351 a          188 c          251 g          384 t
ORIGIN      
        1 aaatgttata aagcttgggt tgctgatgat gaaggtacta aagttgaagt ttggtacact
       61 aaagatattg ttggtaacac caccggtaca cgtcagttta aagaaattga aggttttatg
      121 atgcgttatg aagtacctgt cgcaaatatg gatggcaaca tgaaaatgca atgcaagagt
      181 gtacaagcac aaaaggttag tgatgatatg tttgttatac ctgccggata tgaagtaaaa
      241 tcgcaagagg atttaatgaa aggcaaataa taaaatcaat ttttattgtt atagaagtgg
      301 ctgattccaa tttgaatttg ccactttttt ttaatgacta ttaaatattt aaaaccaaaa
      361 catcatccta tccaaccaac aaaccatgga tcatacgcat aatacacccg cttacacttt
      421 acgccaattg gttttatact ttttaaaatt aggctatgcc ggttttggcg ggccggtggc
      481 attagttggc tatatgcata gcgatttggt tgaaaaaaaa ggatggatta gcgaagaaga
      541 atataaagaa gggcttgcat tagcgcaatt agccccgggg cctttagcag cccaattggg
      601 aatttattta gggtttgtgc attatggcat tttaggtgca accttgtgcg gattggcttt
      661 tgtaattccc tcatttatta tggttgtgtt gattggcata gcctataaaa tatatggcgg
      721 cctgccttgg atgcaagctg tattctacgg tgtaggagct gccgtaattg gcattattgt
      781 tatgagcagt tacaaactca ccatcaaatc gctaagcaaa ttttcaattg tagccataag
      841 agaaaaatgg ctgctttggt tgttctttct cgttgccgga gtaattacaa ttatcaccca
      901 atcggaagat gtattattgt ttgtgtttgc agggttacta tatatgttta taaaagctcc
      961 accggcatgg ttttcaaatc agaaaacaac acaatccatt gtattaaccg gattttcgtt
     1021 tttgcatttc gatagcaata cccttattga cattgcctgg ttttttacga aagcaggtgc
     1081 atttgttttt ggtagtggac tggccatagt tccgtttctg catagtggcg ttgtaattaa
     1141 ataccaatgg ctaactgaaa agcaatttgt tgat
//
LOCUS       DAOMAA010000874         1174 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_159266_length_1174_cov_2.644325, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000874 DAOMAA010000000
VERSION     DAOMAA010000874.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159266_length_1174_cov_2.644325"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="PLO59_10405"
     CDS             <1..895
                     /locus_tag="PLO59_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HQV01562.1"
                     /translation="NRPLKFDEFDSLLHQIIYVSATPADYELEASGGVIVEQLIRPTG
                     LLDPIIEVRPSINQIDDLLGEIDQTIRNKQRVLVTTLTKRMAEELAKYLIKLNIKCRY
                     IHSDIDTLERVEILSELRQGLFDVLIGINLLREGLDLPEVSLVAILDADKEGFLRSNR
                     SLTQTAGRAARNINGKVIMYADKITDSMQRTIDETDRRRAKQMEYNSTHNITPTPVKK
                     STKNYLQSAAQNADKAYVEPNSINVAADPLIKYMDKEQLKKQAAKVKRDMERAARALD
                     FIEAARLRDEMIEIEKLAENI"
     gene            976..>1174
                     /locus_tag="PLO59_10410"
     CDS             976..>1174
                     /locus_tag="PLO59_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01563.1"
                     /translation="MIAGKNANTIFVKRIVGLHFLSLLFFILVGLLQSFAQLSSVRIA
                     ATLLFLCSGIACSGLIWRTGYH"
BASE COUNT          395 a          203 c          237 g          339 t
ORIGIN      
        1 taacaggcca ttaaagtttg acgagtttga tagtttgcta catcagataa tttatgttag
       61 tgctaccccg gccgattacg aactggaagc aagtggcggt gttattgttg agcaattaat
      121 aagacccaca ggtttattag acccaataat tgaagtaaga cccagcatca atcaaattga
      181 cgatttactt ggcgaaattg accaaaccat ccgaaacaaa caacgcgtat tagttactac
      241 acttaccaaa cgtatggccg aagagttagc taaatacctt attaaactca atataaaatg
      301 ccgttacata cacagtgata ttgatacgtt agaacgtgta gaaattttaa gtgaactacg
      361 gcaaggtttg tttgatgttt taattggtat aaacttactg cgcgaaggtt tagatttgcc
      421 cgaagtatct ttagtggcaa ttttagatgc cgataaagaa ggttttttac gaagtaaccg
      481 aagtttgacg caaacagcag gtagggcagc acgtaatata aatggaaaag taattatgta
      541 tgcagataaa attaccgata gcatgcaacg taccattgac gagaccgaca gacgcagagc
      601 caaacaaatg gaatataata gcacccataa cattacacca acacccgtca aaaaatcaac
      661 taaaaactat ttacaaagtg cagcacaaaa tgccgataaa gcttatgttg agccaaactc
      721 cataaacgtt gcagccgacc cattaattaa atacatggat aaagaacaat tgaaaaagca
      781 agcagcaaaa gtaaaacgcg atatggagcg ggcagcacgc gcattagact ttattgaagc
      841 tgctcgcttg cgcgatgaaa tgattgaaat tgaaaagttg gcagaaaata tttaattggt
      901 tttatagtga ataatggcct gtttataatt tgtgtttgca gctacatttt atcattggta
      961 gtagcattga tattaatgat tgccggtaaa aatgcaaata ccatttttgt aaaacgtatt
     1021 gttggcctgc attttttatc gctcttgttt tttatactgg tagggttgtt gcaaagtttc
     1081 gcacaattat catcggttcg tattgccgca acactattgt ttctttgcag tggcattgct
     1141 tgcagtggat taatttggcg cacgggttat catg
//
LOCUS       DAOMAA010000875         1172 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_159773_length_1172_cov_2.726947, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000875 DAOMAA010000000
VERSION     DAOMAA010000875.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159773_length_1172_cov_2.726947"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..>1172
                     /locus_tag="PLO59_10415"
     CDS             176..>1172
                     /locus_tag="PLO59_10415"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HQV01564.1"
                     /translation="MYYVIKNLNTQWLKKLNALLLVLLFSPCQKICAQVLPPFFNDAL
                     VSNNWNQPLGIAFDKNKKLYVWEKAGYVWIVDTNGNKAATPLLDLHEEVGNWVDHGLN
                     SFALHPNFLNNGYIYCFYTVDRHYLLNYGTPAYKSNKNEYGSATIARVVRFTVDMAAG
                     GNTIVPNSKLILIGADKKNGIVITHTSHSGGTLLFGADTSLLISTGDGASFNGIDSGS
                     SVNSFAYQALQDTMLTTANNVGAFKAQMLSSYNGKVLRIDPITGLGLSSNPYFETLNP
                     NSVKSKIWCTGLRNPFRMAIKPNSGSNDITAGDPGVLFIGDVGWFNYEERNISKIG"
BASE COUNT          386 a          193 c          222 g          371 t
ORIGIN      
        1 ttaagtattg acggaaaaga aattttaagt aaaaaacaga ccaccacagc tacgccaaaa
       61 tatacggatg ttacattctc agttaatgat ttggcatctg gaatttactt tataaaaacc
      121 attattaacc ataaaaccat attcggtaaa ctaataattg aataaaactt ttgcgatgta
      181 ttacgtaatt aaaaatctga atacacaatg gttaaaaaaa ttaaatgcac ttctattagt
      241 actgcttttt agtccctgtc aaaaaatatg tgcgcaagtg cttccgcctt tttttaacga
      301 tgccttggta agcaataatt ggaatcagcc tttaggtatt gcttttgata aaaataaaaa
      361 gttgtatgta tgggaaaaag ccggctacgt ttggatagta gatacaaatg gtaacaaagc
      421 tgcaaccccg ttacttgatt tacacgaaga ggtaggcaat tgggttgatc atggtctaaa
      481 cagttttgct ttgcatccta attttttaaa caatggttac atctattgtt tttacaccgt
      541 tgacaggcat tacttattaa actacggtac acctgcatat aaatcgaata aaaatgaata
      601 tggcagtgcc actatagcac gtgtagttcg atttacggtt gatatggctg ccgggggcaa
      661 caccatagtt ccaaacagca aattgatact tattggtgct gataaaaaaa atggcattgt
      721 tataacacat acttcgcaca gtggcggaac tttgcttttt ggtgccgata ccagtttgtt
      781 aatttctaca ggtgatggtg ccagttttaa tggcattgat tcaggtagta gtgttaattc
      841 atttgcatat caagcattgc aagataccat gcttaccact gccaataatg taggcgcttt
      901 taaagcacaa atgcttagta gctataatgg taaagtatta cgtattgacc caattaccgg
      961 cttaggttta tcaagtaatc catactttga aacattaaac cccaattcgg ttaagtcaaa
     1021 aatatggtgt accggtttac gaaatccgtt taggatggca ataaaaccca acagtggtag
     1081 taatgatata acagccggtg atcccggtgt tttatttatt ggcgatgtgg gttggtttaa
     1141 ttatgaagaa cgcaatattt ctaaaatagg tg
//
LOCUS       DAOMAA010000876         1171 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_159972_length_1171_cov_3.228495, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000876 DAOMAA010000000
VERSION     DAOMAA010000876.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159972_length_1171_cov_3.228495"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="PLO59_10420"
     CDS             complement(<1..608)
                     /locus_tag="PLO59_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013928541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit alpha"
                     /protein_id="HQV01565.1"
                     /translation="MIIGVPKETKDGERRVALTPDVIAQLVKAGFTVQVQNDAGVEAY
                     YSNADYTQAGAATCDANTVFAGSDVIVGVNAPAADNIARMKNGAVLISFMFAATNASL
                     VNSCMQAGVSAFSMDAVPRISRAQKMDALSSQSNLAGYKTVLLGANALGKIFPLMMTA
                     AGTIKPAKVVIMGAGVAGLQAIATAKRLGAIVEVSDIRPETK"
     gene            849..1118
                     /locus_tag="PLO59_10425"
     CDS             849..1118
                     /locus_tag="PLO59_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017732976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01566.1"
                     /translation="MIKYTPQYLKKLEEIMSEHEYKVRYEKGNFKSGYCILETKKVVV
                     INKFTPLESRINSLAEIISNLGLSDKNQREETFNFNADSGSTSPA"
BASE COUNT          380 a          237 c          218 g          336 t
ORIGIN      
        1 tctttggttt cggggcgtat atcacttacc tctacaatag cacccaaacg tttggcagtt
       61 gcaatagctt gtaatccggc cacaccggca cccataatta ccaccttggc gggtttaatg
      121 gttcctgccg ctgtcatcat caacggaaaa attttaccta aagcattggc gcccaacagc
      181 acggttttat acccggccaa attactttgc gaacttaggg catccatttt ttgtgcacgc
      241 gaaatacgtg gcacggcatc catactaaat gcactaacac cagcctgcat gcaagaatta
      301 accaatgaag cattggtagc tgcaaacata aatgatatta aaacagcgcc atttttcata
      361 cgggctatat tatcggcagc aggtgcattt acaccaacaa ttacatcgct acctgcaaaa
      421 accgtatttg catcgcaagt ggctgcaccg gcctgtgtat aatcagcgtt gctgtaatat
      481 gcttcaacac cggcatcgtt ttgcacctgt acagtaaaac ctgcttttac aagttgtgca
      541 attacatcgg gcgttaaggc taccctacgc tcgccatctt tggtttcttt aggaactcct
      601 ataatcataa cacttgtttt aagtatttta aaacggataa tgcttacaga ttttgtaagc
      661 gcgcgcaaag aaaacaaata atatgaacgg ctaaagtgta tctgatgctt gtaatttcaa
      721 caatgagatt taagaaacgt ttgattttta ccaagtttaa aattaacagc ttatgtacca
      781 actgttgaaa acataaaaag acagcaatta aaattgatag tattatcgcc atttcaattt
      841 ttggtaagat gataaagtac acgccacaat atttaaagaa attagaagaa ataatgagtg
      901 aacatgagta taaggtgcgt tacgaaaaag gcaatttcaa atcggggtat tgtattttag
      961 aaaccaaaaa agtagtggtt atcaacaaat ttacaccact ggaaagccgg ataaacagtt
     1021 tggcagaaat aatttcgaat ctgggcttaa gcgataaaaa tcaacgtgaa gaaactttta
     1081 attttaatgc cgattccggt tcaacttcac cagcttaatc atgcatttaa aagttacatt
     1141 tttaggtacg ggtacttcgc aaggtgtgcc t
//
LOCUS       DAOMAA010000877         1169 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_160551_length_1169_cov_2.908438, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000877 DAOMAA010000000
VERSION     DAOMAA010000877.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160551_length_1169_cov_2.908438"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1169
                     /locus_tag="PLO59_10430"
     CDS             <1..>1169
                     /locus_tag="PLO59_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01567.1"
                     /translation="TIRSSSATPAWSISQTIPFAFNFNGAPVTNFKVSTTGILTFSVA
                     SAMAPPSSTSQALPSASIPDNSVCVWGLQGTGANDNVVAKTFGTAPNRQHWVFFTSYT
                     IPGNPSGWTYWSIVLEETTNKIYIVDQRTNGTLNMSLGVQINSTTATSVAGSPNINTL
                     SANGATVADNNYYEFNYGTQVALDLSAIDITTNPYVAFGNQDITGTITNLGTATVTSF
                     DINYTINGGAAVTGTINGISIPTLGTYSFTHPVQWNANTPGAFTINVYASNPNGSSDQ
                     NPINDDFSKTINVLSQLEQRNPFFEIFTSSTCPPCEPGNINFHNIIDTIPANQHTYIK
                     YQQDFPGTGDPYTTDESLNRRYYYNINSIPRMENDGGWDGNANSFTYGLYEAARS"
BASE COUNT          367 a          253 c          222 g          327 t
ORIGIN      
        1 ctacaatccg atcatcaagt gctacaccgg cctggagtat ttcgcaaaca attccgtttg
       61 cctttaattt taacggagct ccggttacaa actttaaagt aagtactact ggtattttaa
      121 cttttagtgt tgcatcggct atggcaccac cttcatcaac ttcacaggca ttgccatcgg
      181 caagtattcc tgataattca gtgtgcgttt gggggcttca gggtacaggg gctaacgata
      241 atgtagttgc taaaacattt ggtacggcac ctaaccgtca gcactgggtt ttcttcactt
      301 catacaccat cccgggcaat cccagtggtt ggacgtattg gagcatagtt ttagaggaaa
      361 caacaaacaa aatttacatt gttgaccaac gtaccaacgg taccttaaac atgtcgttgg
      421 gtgtacaaat caatagtaca acagccacaa gcgttgcagg ctcgccaaat ataaataccc
      481 tttcggcaaa tggtgccacc gttgccgaca ataactacta tgagtttaat tatggaacgc
      541 aggtagctct tgatttatca gcaattgata ttacaaccaa tccgtatgta gcatttggca
      601 atcaggatat taccggtaca attaccaact taggtactgc aaccgttaca tcgtttgata
      661 ttaactacac aataaatggc ggtgctgcag ttaccggtac aattaacggt atatcaatac
      721 ccactttagg tacttatagc tttacacatc cggtacaatg gaatgcaaac acacccggtg
      781 cctttactat caatgtttac gcaagcaatc caaatggaag cagtgatcaa aatccaataa
      841 atgatgattt tagcaaaacg ataaatgtgt tatcacaatt agaacaacgc aacccatttt
      901 tcgaaatatt tacaagttca acttgtccgc cttgcgaacc gggtaatata aatttccata
      961 atattattga taccataccg gcaaatcaac atacctatat taagtatcag caagattttc
     1021 cgggtacggg tgatccatac acaaccgatg agagtttaaa tcgcagatac tactacaaca
     1081 taaactcaat acctcgtatg gaaaatgatg gtggatggga cggtaatgcc aactcattta
     1141 cgtatggatt gtatgaagct gcgcgtagt
//
LOCUS       DAOMAA010000878         1169 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_160565_length_1169_cov_2.724417, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000878 DAOMAA010000000
VERSION     DAOMAA010000878.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160565_length_1169_cov_2.724417"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="PLO59_10435"
     CDS             <1..425
                     /locus_tag="PLO59_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009284366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HQV01568.1"
                     /translation="GYEISLDGGLTWIQPNTPSSPTSHGVAQPAQEFLVRALGTFCPT
                     GRTSEPAPCQVVIPNVVTPNGDGKNDTFEMLNLGQYKGAKMSIYNRWGNVIYTSDDYG
                     LANNYDFKDEPDGTYFYSLSIPGQEAKSGTITVFRKQK"
     gene            567..>1169
                     /locus_tag="PLO59_10440"
     CDS             567..>1169
                     /locus_tag="PLO59_10440"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HQV01569.1"
                     /translation="MKVIDHLAKSKTTLFSIEILPPLKGQSIQSIFNSIDPLMEFKPQ
                     FIDVTYHREEYIYKKREGGFLEKISIRKRPGTVGICAAIVNKYKVDAVPHLICGGFSK
                     EETENALIELNFLGIDNVLILRGDAIKTENNFIPHSNGHHYALDLVKQTVNLNKGVYL
                     DEDLQDNENTNFCIGVAGYPEKHFEAPNLQTDLKYLQLKIE"
BASE COUNT          386 a          217 c          221 g          345 t
ORIGIN      
        1 caggttatga aataagctta gatggtggcc taacctggat acaacccaat acgccttcga
       61 gcccaacatc gcacggtgtt gcacaacctg ctcaagaatt tttagtacgc gccttaggta
      121 cattctgccc aacaggccgt acctctgagc ctgctccttg ccaagtagta ataccaaacg
      181 ttgttactcc taatggcgat ggtaaaaacg atacatttga aatgctgaat cttggtcaat
      241 acaagggagc taaaatgtca atctacaacc gctggggtaa tgtaatttac acaagcgatg
      301 actatggttt agcaaacaac tatgatttta aggacgaacc agacggtact tatttctata
      361 gtttaagcat accgggtcag gaagcaaaat cgggtactat taccgtattc cgtaagcaga
      421 aataataaca cgttaaaccc gtaagcgcct tgtaagttaa attgcaaggc gcttttttta
      481 tttatacagc ttatagtttg atagctaagg ctttgtagct tatttatact ttagccaacc
      541 aattttaagt ttataatgcg tgatttatga aagtaattga tcatctggca aaatccaaaa
      601 ccactctttt ttcaattgaa attttacctc cgctaaaggg tcaaagcatt cagtctattt
      661 ttaattccat tgatccttta atggaattta agccgcagtt tatagatgtt acgtatcatc
      721 gcgaagagta tatttataaa aaaagggaag gcggtttttt agaaaaaatt tcaatccgta
      781 agaggccagg taccgttggc atttgtgcag ctatcgtgaa caagtacaaa gttgatgcag
      841 tgccacatct gatatgtggc ggttttagta aagaggaaac tgaaaatgca ttgatagaat
      901 taaatttttt aggcattgat aatgttttga tactgcgagg cgatgccatt aaaactgaaa
      961 ataattttat accacattcg aatggccacc actatgcttt agacttggta aagcaaacag
     1021 ttaatttaaa caaaggcgtg tatttagacg aagatttaca agataacgaa aacactaatt
     1081 tttgcattgg tgttgccgga tatcccgaaa aacatttcga agcacctaac ttacaaaccg
     1141 acctcaaata cctgcaacta aaaattgaa
//
LOCUS       DAOMAA010000879         1169 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_160566_length_1169_cov_2.717235, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000879 DAOMAA010000000
VERSION     DAOMAA010000879.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160566_length_1169_cov_2.717235"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="PLO59_10445"
     CDS             <1..306
                     /locus_tag="PLO59_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01570.1"
                     /translation="TTGRANYLQLGVIKGQRDLFINNPELLEQAEHAVWSACEYWRTR
                     NLDDISNHADDKVLVKKLRSKLYNVSPVEFISMTINGGFNGIEDRKLNYLKAKTVLV"
     gene            complement(346..1056)
                     /gene="kdsB"
                     /locus_tag="PLO59_10450"
     CDS             complement(346..1056)
                     /gene="kdsB"
                     /locus_tag="PLO59_10450"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HQV01571.1"
                     /translation="MQKILGVIPARYASTRFPGKPLVLIDGMPMIERVYKQAILSNAL
                     YQVIVATDDSRIYECVVGFGGKAMLTSALHNSGTDRCAEVALHYNQFDIVVNIQGDEP
                     YIDPQQIDDVCKCFENPEVQIATLIKPILNQEEVNNANVVKCVVSNSYKALYFSRSVI
                     PFQRQGQASYFKHIGIYAYQNNVLQALAQLPASALEQTEALEQLRWIQNDYYIQTAVT
                     TIETIAIDTPADLKKLGI"
BASE COUNT          354 a          238 c          215 g          362 t
ORIGIN      
        1 accacaggcc gcgctaacta tttgcaactg ggtgttatta agggtcagcg cgacttgttt
       61 atcaataacc ctgagttatt ggagcaagcc gagcatgcgg tttggagtgc ctgcgaatat
      121 tggcgtacac gtaatttaga cgatatcagc aatcatgctg atgataaagt actggtaaaa
      181 aaactccgca gcaaattgta caatgtatcg cctgtagaat ttataagcat gaccataaat
      241 ggcggcttta atggtatcga agacagaaag ctgaattacc tgaaagccaa aaccgttttg
      301 gtttaaatct catttaaggt ttttcaatcg ttaaggcttg ttacgttata tacccagttt
      361 ctttaaatca gcgggtgtat ctattgcaat tgtttcgatt gtggtaactg ctgtttgaat
      421 gtagtaatcg ttctgtatcc aacgcaattg ctctaatgct tcggtttgct ctaaagccga
      481 agcaggcaat tgtgccagtg cctgtaatac attgttttgg taagcataaa taccaatatg
      541 cttgaaataa cttgcctgac cttgacgctg aaatggtata acacttcggc taaagtacaa
      601 tgctttataa ctattactaa ccacacactt tacaacgttg gcattgttta cttcctcttg
      661 atttaaaatg ggttttatta aagttgctat ttgcacctcc gggttttcaa aacatttaca
      721 aacatcatca atttgctgtg gatcaatgta aggctcatcg ccctgaatat ttactacaat
      781 atcaaactga ttgtaatgta atgcaacttc ggcacacctg tcggtaccgc tattgtgcaa
      841 tgccgaagta agcatggctt ttcctccaaa acctactaca cattcatata tgcggctatc
      901 atcagttgca acaataacct gatataaagc attggacagt atcgcttgtt tataaactct
      961 ttcaatcatg ggcataccat caattaaaac caagggcttg cccggaaaac gggttgaggc
     1021 ataacgtgcc ggtataacac ctaatatttt ttgcatgcta tttcaagttt attcctggta
     1081 aaattctaac aaagaaccaa agtaactatt atcccaccca tccaacatat cttcaaaagc
     1141 atcgtccgga atattagtat gcaacaatt
//
LOCUS       DAOMAA010000880         1168 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_160785_length_1168_cov_3.427673, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000880 DAOMAA010000000
VERSION     DAOMAA010000880.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160785_length_1168_cov_3.427673"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..1058)
                     /locus_tag="PLO59_10455"
     CDS             complement(90..1058)
                     /locus_tag="PLO59_10455"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017258330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HQV01572.1"
                     /translation="MARIKYHFNPHTLKYEKVIVGVRKRLLIVLGWIATAVVFGALFL
                     WIAYTHFDSPKERQLRNEIDGLKYQYDILQKRVELSETVLHDLENRDDHIYRVIFEAD
                     PIPRSVRQAGYGGIEKYKALQQYDNSRLMVETSKKLERLEKQLYVQSKSFDETLDLAK
                     GKATMLASIPSIQPLSTDDCVIGSGFGYRIHPIYKTSLMHSGIDFTSNIGVPIHATGD
                     GTIIEVAYDGRGYGNHVIINHGYGYESLYGHMSKISVRRGQKVKRGEVIGAVGNTGAS
                     TGPHCHYEVIRNGSKINPINFFYNDLTPEQYQIVIEQASKANQSLD"
BASE COUNT          355 a          253 c          191 g          369 t
ORIGIN      
        1 gccgggttgt atgcaatatg tctcagcacg cccgctgaaa aaacttgcag gtatgtggtt
       61 aagcaataag taatatatca cttataaaat taatcaagcg attgattggc tttactagcc
      121 tgttcaatta ctatctgata ttgctcagga gttaaatcgt tataaaaaaa gtttatagga
      181 ttaatttttg aaccattacg aataacttcg taatggcaat gtggacccgt gcttgcaccc
      241 gtattgccta cagcgcctat tacctcgcca cgctttacct tttgccccct gcgcacactt
      301 attttactca tgtggccgta taacgattcg taaccataac catgattaat gattacgtga
      361 ttgccataac cacgcccgtc ataagcaact tcgataatgg taccatcgcc tgtggcatgt
      421 atgggcacac caatgttaga agtaaagtca atgcccgagt gcataagcga agttttataa
      481 atgggatgta tgcgataacc aaaacccgat ccaattacac aatcgtcagt acttaaaggt
      541 tgtatgctgg gtatgcttgc taacatagta gcctttcctt tagccaggtc taatgtttcg
      601 tcaaacgatt tcgattgcac atacaattgt ttttcgagtc gctccagttt cttcgatgtt
      661 tcaaccatca acctgctatt atcatattgc tgcaatgctt tatatttttc aatgccaccg
      721 taaccagcct gcctcacact gcgcggtatg gggtcggctt caaatattac acgatatata
      781 tgatcatctc tgttttccaa atcatgtaaa acagtttcgc ttaattccac acgcttttgt
      841 aatatatcat attgatattt taagccatca atttcattgc gcaactgcct ttcttttggc
      901 gaatcaaaat gcgtataagc aatccacaaa aataaagcgc caaatacaac agcagtggct
      961 atccatccca aaacaattaa taaacgctta cgtacgccaa caattacctt ttcatattta
     1021 agggtatgtg gattaaaatg atattttata cgcgccattt attgtaactc aaataggtat
     1081 gcatgcaaag gtaattttaa taaccatttt tataaacaca tattaattcg ctttaactac
     1141 ttcttaccga aaacccttta aaatcaag
//
LOCUS       DAOMAA010000881         1167 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_161015_length_1167_cov_7.810252, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000881 DAOMAA010000000
VERSION     DAOMAA010000881.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161015_length_1167_cov_7.810252"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1167)
                     /locus_tag="PLO59_10460"
     CDS             complement(<1..>1167)
                     /locus_tag="PLO59_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01573.1"
                     /translation="ALCKVYYPRYIWVNNVYNVADSAIQYNVIVYDSLNSVWMVDNQK
                     LADLSNYLTTLKYSGIVVTHLEDVFKSADSTQLKNCMAQVVAVLHNAGVPIILNQFRT
                     GEEAMQADSSKNLMLKLLSSSILYNQNVTALQRFDGLLLDYEFWGEDYALDVDFAQIN
                     DGFLYFNEIATEFTAAKANPNNHFKILAMWIGNTVFACDTCDFNGDGLFGDSTIHARP
                     DDEALMNMFDSHGFDRIVLSFFLDDLLDSANTTKFDDPLTFLTRQASGNKINNFFARL
                     YYLGENAYPTYVIPQFHSGKGNGTLSNTKLENYLNGTGPYWGPPYGSGAPHYLHEVEQ
                     TFVGQYFDPNIFDIQNINGNILIVNKNWGTTTIKSGFEWFRYQLGPLDYANLPA"
BASE COUNT          355 a          219 c          225 g          368 t
ORIGIN      
        1 ttgccggtaa attagcataa tctaaaggcc ctaactgata cctaaaccat tcgaagcctg
       61 atttaatagt tgtagtgccc caatttttat tgacaattaa tatattgcca tttatatttt
      121 gaatatcaaa aatattaggg tcgaaatact ggcccacaaa agtttgttct acttcgtgca
      181 ggtaatgggg tgcgccactg ccataaggtg ggccccaata agggccggtg ccattaaggt
      241 agttttctaa tttcgtatta cttaatgtac cattgccctt acccgaatga aattgtggta
      301 ttacataggt agggtatgca ttttcgccca agtaatataa acgggcaaaa aaattattga
      361 ttttattacc actggcctga cgtgttaaaa atgttagcgg gtcgtcaaac ttagtggtgt
      421 tggcagaatc taataaatca tctaagaaga atgaaagtac aattctgtca aagccgtggc
      481 tatcaaacat attcattaag gcttcgtcat cggggcgggc atgtatggtt gagtcaccaa
      541 ataagccatc gccattaaaa tcgcaggtat cgcaggcaaa aacggtattg cctatccaca
      601 tggccaaaat tttaaagtga ttattgggat ttgctttggc agcagtaaat tcagtagcta
      661 tctcgttaaa gtataaaaag ccatcattta tttgtgcaaa gtcaacatcc aatgcatagt
      721 cttcgcccca aaattcataa tcaagcaata ggccatcaaa cctttgtaag gctgttacat
      781 tttgattgta caaaatgctg cttgacaata gtttaagcat caaattttta ctactatcag
      841 cttgcatggc ttcctctccg gttctaaatt ggtttaatat tattggtaca ccggcattat
      901 gtaatacggc taccacctga gccatgcaat tttttaattg tgtagagtcg gcacttttaa
      961 atacatcttc gaggtgtgta actacaattc ctgagtattt taaggtggtt aggtagttgc
     1021 taaggtcggc aagtttttga ttgtcaacca tccaaacact gtttaacgag tcgtaaacaa
     1081 taacgttata ctgtatggcg ctatcggcca cattatacac attgtttacc caaatataac
     1141 gtgggtaata tactttgcat agtgcat
//
LOCUS       DAOMAA010000882         1167 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_161073_length_1167_cov_2.860612, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000882 DAOMAA010000000
VERSION     DAOMAA010000882.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161073_length_1167_cov_2.860612"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(234..>1167)
                     /locus_tag="PLO59_10465"
     CDS             complement(234..>1167)
                     /locus_tag="PLO59_10465"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV01574.1"
                     /translation="AGLRTLTLSFAAPAGCLDSVTYDRTILVNQNGGIGGSSIINDGA
                     IVVFDNAGTPTYANYGCQALVSSLFANAGNDIINCNAGSINLNATVTGNYSSISWGGG
                     SGTFTNINALTTTYTPGVTDTGYFTLSFFATGDCGISSTSYINVYNSAVSPAAVLTAV
                     GSTLYCSLASGTYTYSWTLDGTPTTGSFYIKNATSNGCYQVTITDAAGCSVQSNIVCI
                     TNAGINENPINISVQPNPASNYVLLQLNAATVIGKLSVSIKDVTGKEVMQHNDVVSAL
                     SYKTPLAIQSLAAGVYFIECVTANGTTILKFIKE"
BASE COUNT          360 a          212 c          243 g          352 t
ORIGIN      
        1 tgctgatttt tgttgtgtgt gtgtgatttt ttcataatta ctacgttttt cataaaagat
       61 aaaattaaag ggttaagtga aacttgtttt tggaagtggt aaagataaaa taggagttga
      121 gagttgaaag gggggtaagt gggattaagt tacgttgaat attgaaatgt ttttgccaat
      181 aaaaaaccgg cacaatactg atgccggctt tcaaaattca aaagttgatg tgtttactcc
      241 ttaataaatt ttagtatagt agtaccgtta gctgttacac attcgataaa ataaacaccg
      301 gcagctaagc tttgtattgc taatggagtt ttatacgata atgccgaaac cacatcgtta
      361 tgttgcataa cttccttacc ggttacatct ttaattgaaa cacttagttt accgattaca
      421 gtagctgcat taagttgcag taatacataa ttgcttgcag ggttgggttg cacacttatg
      481 ttaattgggt tttcgttaat acctgcattg gttatacaaa caatatttga ttgcacacta
      541 cagcctgcag catcggttat agtaacctga taacatccat tgcttgtagc atttttaatg
      601 taaaaactac cggttgttgg ggtgccgtct aaagtccagc tatacgtata agtgcccgat
      661 gccaaactgc aataaagtgt tgaaccaact gccgttaata ccgctgctgg actaacagcg
      721 ctattgtaaa cgtttatata cgaagtagaa gaaataccac aatcgcctgt tgcaaaaaag
      781 cttagcgtaa aatagccggt atcggtaact ccgggtgtgt acgtagtggt aagggcatta
      841 atattggtaa aagtaccgct gccaccgccc cagctaatgc tgctgtaatt acctgttacc
      901 gtagcgttta aattgatgga gccggcatta cagtttataa tgtcgttacc ggcattggca
      961 aacaatgacg ataccaaagc ctgacaacca taattagcgt atgtaggcgt accggcatta
     1021 tcaaatacaa caatggctcc gtcattaata attgaactgc cgccaatgcc accattttga
     1081 tttaccagta tggttctgtc gtaggtaacc gaatctaaac aaccggcagg tgcagcaaaa
     1141 cttaaggtta aagtacgcaa accggca
//
LOCUS       DAOMAA010000883         1167 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_161078_length_1167_cov_2.730216, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000883 DAOMAA010000000
VERSION     DAOMAA010000883.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161078_length_1167_cov_2.730216"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..708)
                     /gene="lptC"
                     /locus_tag="PLO59_10470"
     CDS             complement(157..708)
                     /gene="lptC"
                     /locus_tag="PLO59_10470"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HQV01575.1"
                     /translation="MKNLTFVLLLCIAACSNDTDEINRITNEAKSPTETINGLQILYS
                     DSGFVKASMKAAVMNRYYNDTPYTELPKGVAMYFFDIGQDTISTLTAKYAIRNEINRT
                     MEARNDVIVKNKKGETLNTEKLVWNEATGKFTSDVFVKITTASEVIMGQGFEANQDFT
                     RYSIKKPKGSIIVNKSDSTAPAF"
     gene            819..>1167
                     /locus_tag="PLO59_10475"
     CDS             819..>1167
                     /locus_tag="PLO59_10475"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HQV01576.1"
                     /translation="MQYSFTTVWHVMSGIEPVWQAINDVEQWPQWWPGVNKVEQLKPG
                     GVGQINVLTWKSILPYTLKFKATITEVRTHKLITAQATGELEGSGQWVFEQLAGNQTK
                     LTYYWQVRTTKMWM"
BASE COUNT          352 a          206 c          229 g          380 t
ORIGIN      
        1 atctgaatac tgaaaatgca aatgaaatta atgcaataaa aaaatagaga attgaagttg
       61 attttgttgg ttgatttaat tgataaacgc caattgctgc agcggtaatc gtaaggatgc
      121 accaaactat agctaatatg gttaggttag atttgttcaa aatgcaggtg cggttgagtc
      181 ggatttattt actatgatac tgcctttggg ttttttaata ctatagcgcg taaaatcctg
      241 attagcttca aagccttgcc ccattataac ttcgctggca gtagtaattt ttacaaaaac
      301 atcgcttgta aatttgcctg tggcttcgtt ccacaccaat ttttcggtat ttaatgtttc
      361 gcctttttta ttctttacaa ttacatcatt tcgtgcttcc atagttctgt ttatttcatt
      421 acgtatggca tacttagctg taagtgtgct tatggtgtct tgcccaatat caaaaaagta
      481 catggccaca cctttaggca attccgtata gggcgtgtca ttatagtacc tattcattac
      541 tgctgctttc atgcttgctt ttacaaaacc ggaatcgcta tataaaattt gtaaaccatt
      601 aattgtttca gtaggcgatt ttgcctcatt ggttatgcgg tttatttcat cggtatcgtt
      661 actgcatgca gcaatgcata acaataaaac aaatgtcagg tttttcatgg ttggatgtaa
      721 aagtagtttt ttacctgcta taattgatgt gggtgtatgg ccttaattgc aaaccgactt
      781 taatcgaaaa ccgtaagtta gcgtctaaac aaaccattat gcaatatagt tttaccaccg
      841 tttggcatgt aatgtcaggt atagagcctg tatggcaagc cataaatgat gtggaacaat
      901 ggccgcaatg gtggcccgga gtaaataaag tagagcaact taaacccggt ggtgttggac
      961 agataaatgt acttacctgg aaaagcattt tgccctatac tttaaaattt aaagccacta
     1021 ttaccgaagt acgtacccat aaacttatta cagcacaagc cacaggcgag ttagaaggca
     1081 gtggacaatg ggttttcgaa caattggccg gcaatcaaac aaagcttacc tattattggc
     1141 aagtgcgtac aactaaaatg tggatga
//
LOCUS       DAOMAA010000884         1167 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_161086_length_1167_cov_2.651978, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000884 DAOMAA010000000
VERSION     DAOMAA010000884.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161086_length_1167_cov_2.651978"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..>1167)
                     /locus_tag="PLO59_10480"
     CDS             complement(143..>1167)
                     /locus_tag="PLO59_10480"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HQV01577.1"
                     /translation="LLIVFAVNEFNQLPPHTFYWAMVLALLQSIISTHTTMLLAAELV
                     VKQNLVLMIQSFATLLFLLIAFVYNDKLLLHHYLYALLGAYLLATILSTSFMIRHKWL
                     HLNEYSLGWQLIKGVLSYGIVMQSTNICQFLNYRLSFYLLHAQAGKAALGVFSTAIAL
                     GETSWLLSKSLTNVQYPKVVHETNHQFKLLLTNDYAKKAFTGTFCLLLPLLLIPKQGY
                     CLLFGNEFADVKYIVSCLSLGLLLMGFTAPVSHYFSGVSKNRINLYATLTGLIVTVFL
                     GSVLILIFHMQGAILTMLIAYVAIAVVLLFTYCNQTNTSLTVLIPKPSDIKGYINNLF
                     KLSTFK"
BASE COUNT          433 a          207 c          177 g          350 t
ORIGIN      
        1 caaacgcatc tttcgattta ttaaacacat caatagcaac accacctttc aggtgtatca
       61 tatttacaat gtcgtttttc atatttttta tttaatagtg tgttacagtt actataaatg
      121 aacttaaact tgaattaacc ggttatttaa atgttgacaa tttaaacaag ttatttatat
      181 agcctttaat atctgacggt tttgggataa gtacagttaa tgatgtattg gtttgattgc
      241 aatatgtaaa caaaagtaca acagcaatgg ccacataagc tattagcatg gtaagtatgg
      301 ctccttgcat gtgaaaaatt aatattaaaa ccgaacctaa aaaaacagtt acaattaacc
      361 cggttagcgt agcgtataaa ttaattctgt tttttgaaac acctgaaaaa taatgcgaaa
      421 caggcgctgt aaaacccata agtaacaacc ccaaactcaa acagcttaca atatacttaa
      481 catcggcaaa ctcattacca aaaagcaggc agtaaccttg tttaggaatt aaaagtaatg
      541 gtaacagcaa acaaaatgtg cctgtaaaag ccttcttagc ataatcgttt gtgagcaata
      601 atttaaattg atgatttgtt tcatgcacca cttttgggta ttgtacattg gttaaacttt
      661 tgcttaacaa ccaggaggtc tcgccaagtg ctatggctgt actaaacaca cccaatgcag
      721 ctttccctgc ctgtgcatgc aacaaataaa aactgagtct gtaatttaaa aactggcaaa
      781 tattggtact ctgcataaca ataccataac ttaatacacc tttaattaat tgccacccca
      841 aactatattc attaagatgc aaccacttat gtcttatcat aaaacttgtg ctaagtatgg
      901 tagccagtaa ataagcaccc aataatgcat acagataatg gtgcaacaat aatttatcat
      961 tataaacaaa agcaataagt aaaaacaaca gagttgcaaa tgattgaatc attaaaacca
     1021 ggttttgctt tactactaac tcggcagcca atagcatggt tgtatgtgta ctgattatag
     1081 attgaagtaa tgcaagcacc attgcccaat aaaaagtatg cggtggcaac tgattaaatt
     1141 catttacagc aaaaacaatt aataaag
//
LOCUS       DAOMAA010000885         1167 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_161133_length_1167_cov_2.302158, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000885 DAOMAA010000000
VERSION     DAOMAA010000885.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161133_length_1167_cov_2.302158"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="PLO59_10485"
     CDS             complement(<1..341)
                     /locus_tag="PLO59_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01578.1"
                     /translation="MKRFVICLLAIAVIQLPACVSKKKFNAQVAKYDSLKIDNEKNKT
                     LLASCLNDKENQSKRVTSLETELADAKAKGGAMLSQLSDLSVISKEQAESIKRSLENI
                     SGKDAYIKDLQT"
     gene            574..>1167
                     /locus_tag="PLO59_10490"
     CDS             574..>1167
                     /locus_tag="PLO59_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01579.1"
                     /translation="MLLPYFKNTNATIIIRYHCKMEMINEFIYLSVAAGETMPNATCV
                     AQQDNSNLYLTYHLSSNDCLFSFAPIANKVELKISKAYFEKYDTPFEIVSEKQQICCN
                     TQSKLYELINCGIKGIARNIYLESMVLYLLFQVQKNNLIFQLNCDTCTFLNKPIDTDK
                     IKKAKTFIVNNLDQNITIATVANFVGTNQCYLKKGFKE"
BASE COUNT          408 a          209 c          165 g          385 t
ORIGIN      
        1 gtttgcaaat ctttaatgta tgcatcttta ccactgatgt tttctaacga acgtttaatg
       61 ctctcggctt gctccttact tataaccgac aaatcgctaa gctgacttaa catggcacca
      121 ccctttgctt ttgcatcggc aagttcggtt tctaaactgg taacgcgctt gctttgattt
      181 tccttgtcat ttaaacaaga ggccaataat gttttgtttt tttcattgtc aatctttaag
      241 ctatcatact tagctacctg tgcattaaac ttctttttac ttacgcaggc aggtaattga
      301 attacagcaa tagctaataa acaaattacg aatcttttca tatattttaa tttttaattg
      361 cagcaaaaat agcaacccaa acaatcattc atatcaaggc tgtttcatac ttctcaacaa
      421 gttgttatta aaaattcgtt accagttaaa aatcaaaata aagtaccttt taaaccacgt
      481 ccatctaatt tgaataccac atagtacaac agtgctttta ttggctttat tttaaattac
      541 tacaaaggtt ttcaaaaatt tgcaaatgtt attttgttgt tgccgtactt taaaaatacc
      601 aatgcaacta taataattag gtaccattgt aaaatggaaa tgattaatga gtttatctac
      661 ttaagcgtgg cggcaggaga aacaatgcct aacgcaactt gtgttgcaca acaagataat
      721 tcgaacctat atctgactta ccatctaagc agcaacgatt gcctttttag cttcgcgcca
      781 attgcaaaca aagttgagct taaaataagt aaagcctatt ttgaaaagta cgatacgcca
      841 tttgaaatcg tatccgaaaa gcaacaaatt tgttgtaata cacaatcaaa actttacgaa
      901 cttataaatt gtggcattaa aggaatagca cgtaatattt atttagaaag tatggtgtta
      961 tacctgcttt ttcaggtaca aaaaaacaat ctgatttttc aattaaattg tgatacctgt
     1021 acgtttttaa acaaaccaat tgatacggat aaaattaaaa aagccaaaac atttatagta
     1081 aacaacctcg atcaaaatat taccatagca acggtggcaa actttgtagg caccaatcaa
     1141 tgttatttaa aaaaaggttt caaagaa
//
LOCUS       DAOMAA010000886         1165 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_161588_length_1165_cov_3.321622, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000886 DAOMAA010000000
VERSION     DAOMAA010000886.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161588_length_1165_cov_3.321622"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1119
                     /locus_tag="PLO59_10495"
     CDS             <1..1119
                     /locus_tag="PLO59_10495"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HQV01580.1"
                     /translation="DFAMVYGKINQLDKAANYAEQSVAYALKSKSVSHIFEAYEALEY
                     TALLNKNFKKAYEARNFKDSIYLFQNFSENNAKISQIQMAHSFEKQRQQTITTQQLNQ
                     AQAESQLQKQKLIRNFFVVSFCLALLSAFIIFKQRNKIADEKRRSDQLLLNILPAETA
                     DELRKTGAAHAKDFKEVTILFTDFKNFTALSERLTAQQLVNEINYCYSEFDKIISKYG
                     IEKIKTIGDSYMCAGGLPITNTSHAVDCVLAALEMQAFMKKFAEHNLALINKEQSQPT
                     LKWELRIGLHTGPVVAGIVGIKKFAYDIWGDAVNIASRMESSSDPGKVNISGSTYHLV
                     KHEFTCQYRGKIMAKNKGEIDMYYVQQLQSTSQNDSPA"
BASE COUNT          421 a          231 c          216 g          297 t
ORIGIN      
        1 gattttgcga tggtatatgg aaaaattaac cagttagaca aagctgcaaa ctatgccgaa
       61 caaagcgttg cctatgcgct aaagtcgaaa agcgttagtc atattttcga agcctatgaa
      121 gccttagagt acacggcatt actaaataaa aattttaaaa aagcatacga ggcacgtaat
      181 tttaaggata gcatttacct gtttcagaac tttagcgaaa acaatgcaaa aatcagccaa
      241 attcaaatgg cacatagttt cgaaaagcag cgccaacaaa ccattacaac acaacaatta
      301 aatcaagccc aagccgaaag tcaattacaa aaacaaaagc ttatacgtaa tttttttgtg
      361 gttagctttt gcctggcatt actatcggca tttatcattt ttaaacaacg caataaaatt
      421 gctgatgaaa aacgcagaag cgaccaactg cttttaaata ttttacctgc cgaaactgct
      481 gatgaactgc gaaaaacagg agcagcccat gctaaggact ttaaagaagt aaccatactt
      541 tttaccgact ttaaaaactt tacagcttta agcgaacgac taacagcaca gcaattggta
      601 aatgaaataa actattgcta cagcgagttt gataaaataa tcagcaaata cggtatcgaa
      661 aaaattaaaa ccattggcga tagttatatg tgtgccggag ggttacctat taccaacaca
      721 tcgcatgctg ttgactgtgt gcttgcagca ttagaaatgc aagcttttat gaaaaagttt
      781 gccgaacaca acctggcact tattaataaa gaacaaagcc aaccaaccct aaaatgggag
      841 ttacgtattg gcttgcacac cgggcccgtt gttgccggta tagtaggcat taaaaaattt
      901 gcttacgata tttggggcga tgccgttaac attgcttcgc gcatggagtc gagcagcgac
      961 cccggtaaag ttaacattag tggcagcacg tatcatttgg tgaaacatga atttacctgt
     1021 cagtatcgtg gcaaaataat ggcaaaaaac aaaggcgaaa tagatatgta ttacgtgcaa
     1081 caattacaat ccacatcaca aaatgattca ccagcttagt tatcaaacca ccgatttgca
     1141 gttactgcca ctgcaaaaag cacat
//
LOCUS       DAOMAA010000887         1165 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_161598_length_1165_cov_3.193694, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000887 DAOMAA010000000
VERSION     DAOMAA010000887.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161598_length_1165_cov_3.193694"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..605)
                     /locus_tag="PLO59_10500"
     CDS             complement(66..605)
                     /locus_tag="PLO59_10500"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HQV01581.1"
                     /translation="MRIISGKLKGKTIVAPKHLPVRPTTDFAKTGLFNILNNQYQFET
                     LSILDLFTGTGAISYEFASRNCENILAIDMHAACVKFVNSVFKKWQCIGCEAQQSDAF
                     LFLKKCNESFDIIFADPPYDLPQIENLVTLILDRNLLKPRGVFILEHEHNRPIQTKIL
                     PDDVRNYGNVSFSFFKSTT"
     gene            complement(596..>1165)
                     /locus_tag="PLO59_10505"
     CDS             complement(596..>1165)
                     /locus_tag="PLO59_10505"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HQV01582.1"
                     /translation="AFYEAQKNAELLAFNHQIGSADSIAKDDFGFFDAKHVFAVNDDL
                     INFAVNQLKQVHINHCGTAFIEHGLLKNRNKAQRAISVNVHKHQFEMMVIAENQLLFY
                     NNFYYNTAEDFIYFIMLVYEQLNLNPEEDVLEITGLIEKQSAIFSISKKYIRYVDLAM
                     RQQAFQFSYGFEQLPAWQFQTLFSLGLCV"
BASE COUNT          380 a          209 c          182 g          394 t
ORIGIN      
        1 cgattgctcc gaaaaatcga taggtacaag tatgtgatta ctactggtta tcatatatta
       61 tattgttaag ttgttgattt aaaaaacgaa aatgaaacgt taccataatt tcgcacatca
      121 tctggtaaaa tctttgtttg aattggcctg ttatgttcat gctccaatat aaatacaccc
      181 ctcggtttta acaaatttct atctaaaatc agtgttacta aattttcaat ttgtggcaaa
      241 tcgtaaggtg ggtcggcaaa gataatatca aatgactcgt tacatttttt taaaaacaaa
      301 aatgcatcgc tttgttgtgc ttcacaacca atacactgcc actttttgaa aacactgttt
      361 acaaatttta cacaagctgc atgcatatca attgctaata tgttttcaca attacgtgat
      421 gcaaattcat aactgatagc acctgtgcct gtaaataagt cgagtatgct cagcgtttca
      481 aattggtatt gattatttaa aatattaaac aaaccggttt ttgcaaaatc ggtagtgggt
      541 cgcaccggca agtgctttgg tgcaacaatt gttttacctt ttagcttacc actaattata
      601 cgcacaagcc taaactaaat aatgtttgaa attgccatgc cggtaattgc tcaaaaccgt
      661 aactaaattg aaatgcctgt tgtcgcattg ccaaatcaac atagcggata tattttttac
      721 tgatactaaa gattgccgat tgtttctcaa ttaagcccgt aatttccaaa acatcttctt
      781 cggggttaag atttagctgt tcataaacca gcatgataaa ataaataaaa tcttcggctg
      841 tgttgtagta aaagttgttg taaaacagca actggttttc ggcaataacc atcatttcga
      901 attgatgctt atgtacattt accgatatag cgcgctgtgc tttatttcga ttttttaata
      961 aaccatgttc aataaaagca gtaccgcaat gatttatatg aacttgcttc aactgattga
     1021 ctgcaaaatt tattaaatcg tcattaacag caaaaacatg ttttgcatca aaaaatccga
     1081 aatcatcctt tgcaatacta tctgcacttc ctatttgatg gttaaaagcc aaaagctcgg
     1141 catttttttg tgcttcataa aatgc
//
LOCUS       DAOMAA010000888         1165 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_161630_length_1165_cov_2.830631, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000888 DAOMAA010000000
VERSION     DAOMAA010000888.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161630_length_1165_cov_2.830631"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="PLO59_10510"
     CDS             <1..643
                     /locus_tag="PLO59_10510"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQV01583.1"
                     /translation="PYSMVYYSEVIALFIKKMQLTDVTICGHSMGGQVALIFALRYPQ
                     LAQKLILIAPSGFEQFTAFEIAAIKQMLPLSNWFISDETKLVQSLKQSFFKLNATGSQ
                     IINDLQGLLSQTDLAQWRYMVNRCIVGMLTEPVYDLLPFLASKTLVIFGCNDALIPNT
                     MLHYSTTQKIAQRACKQIPDAQLHMIEHAGHAVFLEKPDEVNRLIIDFMNVSD"
     gene            738..>1165
                     /locus_tag="PLO59_10515"
     CDS             738..>1165
                     /locus_tag="PLO59_10515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HQV01584.1"
                     /translation="MSVNPSDDWIAKQKQFMNLYEPLHDRLSRFVYAMVWDREEARDV
                     ISDTILAAYESFEKVRNHQAFLSYLFTIASRTWSKKVRKAQMVTALTEVHTNIIADEA
                     SNIEARSELNEFYAALSTLPEKQREAITLFEISGLSLNEIV"
BASE COUNT          369 a          203 c          237 g          356 t
ORIGIN      
        1 gccttatagc atggtttatt attcggaagt aattgccttg tttataaaaa aaatgcaatt
       61 aacggatgtt accatttgtg gtcacagtat gggtggacaa gtagctttaa tttttgcact
      121 ccgttatccg caattagcac aaaaacttat attgattgcg ccatcgggtt ttgagcaatt
      181 tacggctttc gaaattgctg ctattaaaca aatgttgccc cttagcaatt ggtttatttc
      241 agatgaaaca aaactggtac aaagcctcaa acaaagtttt tttaaactga atgcaaccgg
      301 cagtcagatt attaatgact tacaaggttt gctttcgcaa acagatttgg cgcaatggcg
      361 gtatatggta aaccgttgta tcgtaggcat gctaaccgaa cctgtttatg atttgttacc
      421 atttctggca tccaaaacat tggtaatttt tggatgtaac gatgcattaa taccaaatac
      481 catgttacat tacagtacca ctcaaaaaat tgcgcagcgt gcgtgcaaac aaatacctga
      541 tgcacagttg cacatgattg aacatgccgg acatgccgtg tttttagaaa agcccgatga
      601 ggtaaacagg ttgattattg attttatgaa cgtttctgat taggtgttta ttggggttaa
      661 acagcacatt tttatttatt tcgtgcaact ttttatcaca aatactatct taccatttaa
      721 agcggcttat tacggatatg agtgttaacc ccagtgatga ttggattgca aaacaaaagc
      781 agtttatgaa tttatatgaa cccttgcacg acagattgtc gaggtttgtg tatgccatgg
      841 tatgggatag ggaagaagca cgtgatgtta ttagtgatac cattttagca gcctacgaaa
      901 gttttgaaaa agtaagaaac catcaggctt ttttaagtta cttatttacc atagccagcc
      961 gaacgtggtc gaagaaggtt agaaaagcac aaatggtaac agcacttacc gaagtacata
     1021 caaatattat cgcggatgaa gcatcaaaca ttgaagcacg ctcagagctg aacgagtttt
     1081 acgcagctct gagcactttg cccgagaaac aaagagaagc gattacatta ttcgaaattt
     1141 cgggattatc gctaaatgaa attgt
//
LOCUS       DAOMAA010000889         1165 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_161656_length_1165_cov_2.530631, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000889 DAOMAA010000000
VERSION     DAOMAA010000889.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161656_length_1165_cov_2.530631"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            304..>1165
                     /locus_tag="PLO59_10520"
     CDS             304..>1165
                     /locus_tag="PLO59_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HQV01585.1"
                     /translation="MFYNIKKTAAFILAATVAGYTGAYVFNKTNNKQAYTVAPATPVK
                     FTSGNAALPTATDFTDAANRTVHGVVHIKTSYSMQQTAYSPFELFFGGGGGRQPQAST
                     GSGVIISNDGYIVTNNHVVDAAEKVEVTLSDNKKYEGKVIGADPSTDLALIKIDAQNL
                     PFIPFGNSDDVQVGQWVLAVGNPFNLTSTVTAGIVSAKGRNINILANNGSNVAPIESF
                     IQTDAAVNPGNSGGALVNTNGELIGINSAIASNTGSYAGYSFAVPVNIARKVIDDFME
                     YGTIQRAYIGI"
BASE COUNT          397 a          209 c          222 g          337 t
ORIGIN      
        1 ctaaattggg ggtgcatttg catgccgtca atcaaaataa aatcattgcc agcaccttgg
       61 tatttataaa atcgaaacat ttataatcat ttaaatattt gcttactgta aaggtagaaa
      121 ggttaagttg cagttaacca tatcatctat taaaatcttg taacacatca atacgttagt
      181 aggataaaat attagttttt tgattaacac tttgctaact acaactttca caaagcattc
      241 atacaacaac aacaaatttg caatcaataa tttaataaca ccaaaataat aaatcttaaa
      301 accatgtttt acaacattaa aaaaacagca gcatttatat tagcagcaac ggtagcagga
      361 tataccggtg cttatgtatt taataaaacc aataataaac aggcttatac agtagcacct
      421 gctacaccgg taaaatttac atcgggcaat gccgccttac ctacagcaac agattttact
      481 gatgccgcaa accgcaccgt gcatggtgta gtgcatatta aaacttcgta cagtatgcag
      541 caaacggcat acagtccgtt cgaattattt tttggtggcg gtggtggcag gcaaccgcaa
      601 gcatcaaccg gttcgggtgt tataataagt aacgatggtt atattgttac caacaatcac
      661 gtagtggatg ctgccgaaaa agtagaagtt actttaagtg ataataagaa gtatgaaggc
      721 aaagtaatag gtgccgaccc aagtaccgat ttggcattaa taaaaataga tgcacaaaat
      781 ttacccttca ttccatttgg taatagtgat gatgtacaag ttgggcaatg ggttttagcc
      841 gttggcaatc catttaacct tacttcaact gttactgcag gtattgttag tgcaaaggga
      901 aggaacataa atattttagc aaataatggc agtaatgtag caccaatcga atcgtttata
      961 caaaccgatg cggctgttaa cccgggcaac tcaggcggtg cattggtaaa taccaatggc
     1021 gagttgattg gaataaactc tgccattgca tcgaataccg gtagctatgc aggttattca
     1081 tttgctgtgc ctgtaaatat tgcacgtaaa gttattgatg attttatgga gtatggtacc
     1141 atacagcgcg catatattgg catac
//
LOCUS       DAOMAA010000890         1165 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_161691_length_1165_cov_2.264865, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000890 DAOMAA010000000
VERSION     DAOMAA010000890.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161691_length_1165_cov_2.264865"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1165)
                     /locus_tag="PLO59_10525"
     CDS             complement(<1..>1165)
                     /locus_tag="PLO59_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01586.1"
                     /translation="LDGNGVVQWSNSFNLTGYFYYAPVEVADGYVFNNQLPGNAVAFY
                     KTDKQGNVQWGGLKRTNLQGPLSPLQNNGNHIIASFVTATTPATVSIYEFDSALQVIR
                     QYGVNTLASGYAIKPAALLADGSLLNAGFNNNILQLNKLNQTGNAPCAAAPLTVTQNV
                     EVYSKSAGNAVATSHAFTRTALTITTDTFSLNDIAICTQNKYLNLGADTFACGNINYI
                     IKNQLPDSFETYLWSTGATTSQIQATTAGTYTLTVTYLCGEKSLTDSIHISYQTAPVI
                     GFADSIFLCEDSLLQLVGPYCTNCSYLWNTGDTTSTITATHAGTYAVTVTSPNGCQAT
                     TSHTISAHPLPVVYFLGLPDSTCLDANPVTLTGAPTMGTFSGQGILGNDFDASV"
BASE COUNT          338 a          230 c          250 g          347 t
ORIGIN      
        1 aactgatgcg tcgaagtcgt tacccaatat gccttgtcct gaaaaagtac ccattgtagg
       61 tgcgccagtc aacgtaactg gattggcatc gaggcaggta ctatctggaa gccctaggaa
      121 atagacgact ggaaggggat gagccgagat cgtatgtgaa gtagtagcct gacatccatt
      181 gggacttgtt acagtaactg cgtatgttcc tgcatgcgtg gcggtgattg ttgatgtggt
      241 atcgccagta ttccacaaat aagaacaatt ggtacaatac ggcccaacta actgtagcaa
      301 actatcttcg cataaaaaaa tactgtcggc aaaaccaata acaggtgctg tttgataaga
      361 aatatgaata ctgtcggtta aacttttttc gccacacaga tatgtaacag ttagtgtata
      421 ggtgccggct gttgtagctt gtatttgaga ggtggtggct cctgttgacc acaaatacgt
      481 ttcgaaacta tcgggcaatt gatttttaat tatgtaatta atattgccgc atgcaaaagt
      541 atcggcaccc aaattcagat acttgttttg tgtacaaata gcaatatcgt ttaatgaaaa
      601 agtatctgtt gtaatagtta aggcagttcg tgtaaatgca tgcgaagttg ccacggcatt
      661 acctgcactt ttcgaataaa cttcaacgtt ttgtgtaacc gttagtggcg ctgccgcgca
      721 tggtgcatta cctgtttgat tcagtttgtt caactgtagt atattgttgt taaaacctgc
      781 atttaataaa ctgccatcgg ctaatagtgc ggctggttta atggcatagc ccgatgccaa
      841 tgtgttaact ccatattgac gaataacctg caatgccgaa tcaaattcat atatagaaac
      901 agttgcaggc gttgttgctg ttacaaatga ggcaattata tggttgccat tattttgaag
      961 cgggcttagt ggtccttgta aattagttcg tttaagccca ccccattgca cattgccttg
     1021 cttatcagtt ttataaaatg caactgcatt gcccggcaac tgattgttaa acacataccc
     1081 gtctgcaact tcaaccggtg cataataaaa ataaccagta agattaaagc tgttagacca
     1141 ctgcaccact ccattaccat ctaat
//
LOCUS       DAOMAA010000891         1163 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_162125_length_1163_cov_3.164260, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000891 DAOMAA010000000
VERSION     DAOMAA010000891.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162125_length_1163_cov_3.164260"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1163)
                     /locus_tag="PLO59_10530"
     CDS             complement(<1..>1163)
                     /locus_tag="PLO59_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01587.1"
                     /translation="IIAAGWNDLLATGPDFSYTTVGTAPNRKLVVNYNVRHYLSATHI
                     FKLQIQLHETTNIIELHTDTIPDVSSVSGSATTTQGIENANGTQAVTVPGRNSQHFNA
                     DNDAYRFTPYLAINAQWNPGALNGLTQNVIPAASTTYSVTVNNGLGCTSTATVNVTVH
                     NNPAPVISGNLAFCAGGSTLLDAGSFANYQWSNGETTQTINLNTPGTYTVTVTDANGC
                     TASVSANVIQHALPTPSISGVLTFCAGNNTTLDAGLYVSYQWSNGETTQTVLVNTAGA
                     FTVTVTDANGCTATASATTIVSNALMPGISGNTQICVNGNTTLLCNGIFDSYLWSNGN
                     TTNSISVNTTGTYTITVSDNQGCTGTASAIVTNYATVSCNISGPAQYCASGNV"
BASE COUNT          305 a          226 c          262 g          370 t
ORIGIN      
        1 tacattacca ctcgcacagt attgagcagg ccctgaaatg ttgcatgata ctgttgcata
       61 atttgtaaca attgcagatg ctgtacccgt acaaccctga ttgtcagaca ctgtaattgt
      121 ataagtgcct gttgtattca cacttattga attggtggta ttgccgttcg accatagata
      181 cgagtcaaaa ataccattac acaaaagggt tgtattgccg tttacacata tttgtgtgtt
      241 gccgctaata cctggcatta aagcgttgct tacaattgtt gttgccgatg ccgttgctgt
      301 acagccgttt gcatctgtta cagttacagt aaatgcaccg gctgtattaa ctaaaacggt
      361 ttgtgttgtt tcgccattag accattgata gcttacatat aaaccggcat caagtgtagt
      421 attattgcca gcacaaaaag ttaaaacacc tgatatggat ggggttggta gtgcatgctg
      481 aattacgttt gccgaaacag aggccgtaca gccatttgca tcagtaacgg ttactgtata
      541 ggtgccaggt gtattcaggt taatggtttg tgttgtttcg ccattagacc attgataatt
      601 tgcaaagcta cctgcatcta atagggtact gccaccggca caaaatgcca aattgccgct
      661 tattacaggc gcaggattat tgtgcacggt aacatttact gttgccgtac tggtgcagcc
      721 taatccattg tttactgtaa ccgaataggt tgttgaagca gccggtatta cattttgtgt
      781 taggccgttt aatgcacccg ggttccattg tgcgttaatt gccaaatagg gtgtaaacct
      841 gtatgcatca ttatcggcat taaaatgttg gctgttacga ccgggtacag taacggcttg
      901 cgtaccgttt gcattttcaa taccctgtgt tgttgttgca ctgccgctta ctgacgaaac
      961 atcgggtatg gtatctgtat gtagctcaat aatattagtg gtttcgtgaa gttgaatttg
     1021 caatttgaaa atatgtgtgg ccgataggta atgccttaca ttataattta ctactaactt
     1081 acggttgggt gcagttccaa cggtagtgta gctgaaatcg gggcctgttg ctaataaatc
     1141 attccaaccg gcagcaatta tat
//
LOCUS       DAOMAA010000892         1162 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_162418_length_1162_cov_2.803975, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000892 DAOMAA010000000
VERSION     DAOMAA010000892.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162418_length_1162_cov_2.803975"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="PLO59_10535"
     CDS             complement(<1..602)
                     /locus_tag="PLO59_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01588.1"
                     /translation="MKKITYLLVTVSTLLCLQAQAQFISGGAGKTILQNNGDLVGIGT
                     TNPAFVLDVQRAGNASMNFKSTTGTANLILDRANSASTSSVSYRTNGSPTWQTGTLGT
                     DNFAIRNIALGNSSLSVTAATNYIGIGTNAPASRLHVNDNGLAELKITSTTNGTKLSL
                     DRTANGYEAVTQYSQTGVPQWKTGLLVNNSGAAEYVIKNE"
     gene            complement(716..>1162)
                     /locus_tag="PLO59_10540"
     CDS             complement(716..>1162)
                     /locus_tag="PLO59_10540"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQV01589.1"
                     /translation="NSMYDIIWSINPANDSMNDLTTRMRSYASTLLEAKNIAYEINMP
                     DFANKQMLTLEVKNHIYLIFKEAVNNAAKYAQCNHLKINLHFYKKKLTLNITDNGVGF
                     DVGATLNPGNGYGLANMRYRAQAINGTLHIASYKTQGTTIVLTVQL"
BASE COUNT          307 a          214 c          246 g          395 t
ORIGIN      
        1 atttcgtttt taataacata ctcggcagct ccactattgt ttaccagtaa acctgttttc
       61 cattgtggta ctcctgtttg tgagtattgg gttactgctt cgtaaccatt agccgtacgg
      121 tctaacgata atttggtgcc attggtggta gaagtaattt taagctctgc cagtccattg
      181 tcgtttacat gcaacctgga ggcaggtgca ttggtgccta taccaatata attggttgca
      241 gcagttaccg ataacgatga gttgcccaat gcaatgtttc tgattgcaaa attatcggta
      301 cccagtgtac ctgtttgcca tgtggggcta ccattggtgc gataacttac tgacgaggtt
      361 gaggcgctgt tagcacggtc taaaatcagg ttagcggtac cggtagttga tttaaagttc
      421 atactggcat tgccggcacg ttgtacatct aaaacaaatg ccgggttggt tgtgccgata
      481 ccaaccaggt cgccattgtt ttgtaaaata gttttgcctg caccgccact aataaattgc
      541 gcctgtgctt gtaagcataa tagggtgctt acggttacca ataagtaggt gatttttttc
      601 ataagattaa gtaatttttg tttgacgaat atacgttgtc tgcaagcaaa agcaccccca
      661 taaacatgta ggttgtttat gggggttaag cattgcaaat ttgttttaac agttactata
      721 actgcacagt taaaacaata gtagttccct gtgttttgta tgaggctatg tgcaatgttc
      781 catttatagc ttgggcccta taccgcatat tggccaagcc atatccattg ccagggttta
      841 aagttgcacc aacatcaaag cctacaccat tatcagttat attaagggta agttttttct
      901 tgtaaaaatg caggttaatt ttcaggtgat tgcattgggc atattttgca gcattgttaa
      961 cggcttcttt aaaaattaaa taaatatggt tcttcacttc taatgtgagc atttgtttat
     1021 tggcaaagtc gggcatgttt atttcgtatg caatattttt tgcctccagt aaagttgatg
     1081 cataactacg catgcgtgta gttaaatcgt tcatcgaatc gttagcagga tttatgctcc
     1141 aaattatatc atacatactg tt
//
LOCUS       DAOMAA010000893         1161 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_162690_length_1161_cov_2.750452, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000893 DAOMAA010000000
VERSION     DAOMAA010000893.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162690_length_1161_cov_2.750452"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="PLO59_10545"
     CDS             complement(<1..282)
                     /locus_tag="PLO59_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01590.1"
                     /translation="MVSIFLCYKCIAQNQIRTTTLENNNWVIYTVFVDVNKNGYFDNQ
                     EMVLPNHMLINDGAYYVTDSKGQFKAKTNITGTIKSSNKNFEVNNVAAQS"
     gene            complement(440..>1161)
                     /locus_tag="PLO59_10550"
     CDS             complement(440..>1161)
                     /locus_tag="PLO59_10550"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HQV01591.1"
                     /translation="NTFVKRGATNPADGQHQIIISIRKDNTCECIAGRIEVKDGKIKQ
                     PLYVKNADGTYRVTQKKLSKNTGQGDHSFGQLATVVKGMSRTFQTSDYEVINLFFIEC
                     LNPLPATKTDVTNSDTANNSATKTAALAIANNLVFSTGSTDLQKSSIAGLEQLADAMT
                     NEPATQISIVGHTDNDGSIAANIVLSENRALAVKAFLESKGIDSKRILTFGYGESKPI
                     ASNRTAAGRSLNRRIEIITQY"
BASE COUNT          322 a          214 c          213 g          412 t
ORIGIN      
        1 agactgggca gctacattat ttacctcaaa atttttatta ctgcttttaa tagtaccggt
       61 aatatttgtt tttgctttga actgaccttt actgtcagtt acataatatg caccatcgtt
      121 tattagcata tgattaggca aaaccatctc ctggttatcg aagtaaccat tcttgttaac
      181 atcaacaaaa acagtatata ttacccaatt gttgttttct aatgttgttg ttcttatttg
      241 gttttgggct atacatttat agcataaaaa tatggaaacc aaaattgcaa tcgccagttt
      301 aagtttattc atggcatagt aaattacaaa tgagttaaac aaacttaaac aaaaacttgg
      361 atgttgcaat acgttaccaa atcaaaattt tgatacccaa acaatgttgg taggatttta
      421 attaaatgga agcctaatac taatactgcg taattatttc aatacgcctg tttaagctac
      481 gccctgctgc tgttcgattg ctggcaatag gtttcgattc gccatagcca aaagttaata
      541 tgcgtttgct gtctatacct ttcgattcta aaaaagcttt tactgccaaa gcgcggtttt
      601 cagataaaac gatgttagcc gctatgctgc cgtcattgtc agtgtggcca acgatggaaa
      661 tttgggttgc aggctcgttg gtcattgcat cagccaattg ctcaaggccg gcaatcgaac
      721 ttttttgtaa gtcggtactg ccggttgaaa aaactaaatt gttggctata gctaatgctg
      781 ctgttttggt tgcactattg tttgccgtgt cgctatttgt tacgtctgtt ttagttgccg
      841 gcaacggatt taaacattca ataaaaaaca agttaattac ttcataatcg ctggtttgaa
      901 aagtgcgcga catgccctta actacggttg ctaattggcc aaatgaatga tcgccctgcc
      961 cggtattttt actcaatttt ttttgagtta ctcgataggt tccatctgca ttttttacgt
     1021 acaatggttg ttttattttg ccatctttta cttcaatcct gcctgcaata cattcgcagg
     1081 tgttatcttt tcgtatagaa ataataattt gatgttggcc atcggccgga ttggttgcac
     1141 ctcttttaac aaaggtgttg t
//
LOCUS       DAOMAA010000894         1158 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_163498_length_1158_cov_2.541251, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000894 DAOMAA010000000
VERSION     DAOMAA010000894.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163498_length_1158_cov_2.541251"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1158
                     /locus_tag="PLO59_10555"
     CDS             <1..>1158
                     /locus_tag="PLO59_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01592.1"
                     /translation="EAGIGIQGSFYGDLYYNALRSKTFNAGIHAKHFSSAGKINDYGF
                     PGNSLNLIETFGEKYLEHGTIGAKFGYQRNVFHYYGYDAEQTVIAKSETRHVFNDISG
                     SVDYKSLLLSSENLKPNVGIDFHHFADNLNSSESRFGLRSFWQKQIASGSADVTLNFA
                     FTKYKQPLRNFNRSRFTLTPTYTFSYLPWTFKLGFNTEMEGETETKFRLYPYARFDYA
                     LLDKVTVFGELSGKVADNNFKSISTDNPFLEDTISVLNTNQLRNIKFGVHARIDNQFT
                     FNAYVNFAKQKNALFYANRFTAFQPVTFIPVYTAASLTAVHAELGFENSTKLNVWLKA
                     DYLGYSKFDTSTIKSWHVSPFMFTLALNYNLSDKIMVKTDVFIRSESERIKY"
BASE COUNT          372 a          199 c          217 g          370 t
ORIGIN      
        1 gaagcaggta ttggtattca aggttctttt tatggcgatt tatactacaa tgccttgcgc
       61 tcaaaaactt ttaatgcagg catacatgca aaacactttt catcagcagg taaaataaat
      121 gactacggct ttccgggcaa ctccttaaat ttaatcgaaa cctttggcga gaaatattta
      181 gaacatggta ctataggtgc aaagtttggt taccaacgca atgtatttca ttattatggt
      241 tatgatgccg agcaaaccgt aattgcaaaa tcagaaacac gccatgtgtt taatgatata
      301 agtggttctg ttgattataa gagtttgctt ttaagttctg aaaatttgaa accaaatgtt
      361 ggcattgatt ttcatcattt tgccgataat ttaaacagta gcgaaagccg ttttggctta
      421 cgcagctttt ggcaaaaaca aattgcaagc ggtagtgccg atgttacatt aaattttgct
      481 tttactaaat acaagcagcc acttcgaaac tttaaccgtt cgcgttttac attaacccca
      541 acctatactt ttagctattt accctggaca tttaagctgg gctttaacac agaaatggag
      601 ggtgaaaccg aaaccaaatt cagactttat ccttatgcgc ggtttgatta tgcattgtta
      661 gataaagtaa ccgtattcgg tgagttatcg ggcaaggtgg ctgataataa ttttaaaagt
      721 attagcacag ataatccgtt tttagaagac accatctctg tattaaatac caaccaatta
      781 cgtaatatta aatttggggt tcatgcacgt attgataatc aatttacttt taatgcatac
      841 gtaaactttg ccaagcaaaa aaatgcattg ttttatgcca acaggtttac agcatttcag
      901 ccggtaactt ttatccctgt ttacactgct gcaagcttaa cagccgtgca tgcagagttg
      961 ggatttgaaa acagcacgaa gctaaatgtt tggctaaaag ccgactattt gggttatagc
     1021 aagtttgata caagtacaat caaaagctgg cacgtttcgc cttttatgtt tacactggca
     1081 ttaaactata atttaagtga taagattatg gttaaaaccg atgtgtttat caggtcagaa
     1141 agtgaaagga taaaatat
//
LOCUS       DAOMAA010000895         1157 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_163728_length_1157_cov_3.634301, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000895 DAOMAA010000000
VERSION     DAOMAA010000895.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163728_length_1157_cov_3.634301"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(269..>1157)
                     /locus_tag="PLO59_10560"
     CDS             complement(269..>1157)
                     /locus_tag="PLO59_10560"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HQV01593.1"
                     /translation="SIYRPKNRFKKTMSLSMFRSVAILFCLFIHLIAMGQSSEIGITL
                     GTSTYKGDLKTTMFTTKYNHLAGGVLFRHSNSNHWSYKVALHAGRISAADSTSKSDIQ
                     KYRNLSFRSNWQELNLQTEFNFFKFQTANPTSTYTPFLALGLTLFHFNPQALLNDEWV
                     DLQPLATEGQGTSQFGDRKKYKRLQVALAFGGGFKFRLSRRFGLVVESGVRRTFTDYL
                     DDVSTTYADKKILAATYGTDAALLSDRSVNQGNNQNMLRDRGNRNDKDWYVFTGVQLT
                     YTISKKYNDVCKPFRIKFK"
BASE COUNT          329 a          210 c          247 g          371 t
ORIGIN      
        1 aaggcgataa aatttattat ggcaggtttg tgaaggagtg attagtggtt agtggatagt
       61 gattagtggt gtgtgggatg cctttgctat ggtttatttg taaattatca ccgtttgtag
      121 cttcagtaaa caatttttgc tatgggtata gttctggcaa gggcgtgcac tttcatttat
      181 tatacgacaa ttgggtaatg gcctttttca caaggcctaa gtcaaaacta taaatggctt
      241 cattgtttta aatcaagcac taaaaacact acttaaattt aatcctgaat ggcttacaaa
      301 catcgttata ctttttactg atggtgtaag taagttgcac accggtaaac acataccagt
      361 ctttatcgtt gcggttgccg cggtcgcgga gcatgttttg gttattgccc tgatttaccg
      421 acctatcgct aagtaaagcg gcatcagtgc cataggtagc agccaatatt tttttatcgg
      481 cataggtagt gcttacatcg tccagataat cagtaaaagt tctgcgtaca ccactttcaa
      541 ccaccaaacc aaacctgcgg cttagcctga atttaaaacc accgccaaat gctaatgcaa
      601 cttgcaggcg tttgtatttt tttcgatcgc caaattgcga tgtgccctgg ccttctgttg
      661 ctaatggttg caggtcaacc cactcatcgt ttaaaagtgc ttgcgggtta aagtgaaaca
      721 aggttaaacc caaagctaaa aacggtgtat aggttgaggt tgggttagcg gtttggaatt
      781 tgaaaaaatt aaattcggtt tgcaggttta actcttgcca attactgcga aacgataagt
      841 ttctgtattt ctgtatatcg ctctttgaag ttgaatcggc agcagaaata cgacctgcat
      901 gtaatgctac tttatacgac cagtgattgc tgttgctatg cctaaataaa acaccaccag
      961 ccaaatggtt gtatttggtt gtaaacatgg tagtttttaa atcgccttta taggttgatg
     1021 tacccagggt tatgccaatt tcagacgatt gccccattgc aattagatga atgaataaac
     1081 aaaacagtat ggcaacactg cgaaacatgg ataaactcat tgttttttta aaacggtttt
     1141 ttggccgata tattgag
//
LOCUS       DAOMAA010000896         1157 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_163811_length_1157_cov_2.544465, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000896 DAOMAA010000000
VERSION     DAOMAA010000896.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163811_length_1157_cov_2.544465"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1157)
                     /locus_tag="PLO59_10565"
     CDS             complement(<1..>1157)
                     /locus_tag="PLO59_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01594.1"
                     /translation="TYASRIGSFANVNGTIPGGLAWNMFYRPTYAVIRAGNPSNLNIT
                     ATAGPNGAISPSGVINIVYNGSQTFNMTPNANYFIDSVFVDGNYIGTPSTYTFNNVTV
                     NHTIHVQFKTNSYTINASKVGAGVISPSGTINVMQGGSQSFSFTPNNTCTKIDSVIVN
                     GIFIGTPASYTFNNVASNHTIKVVFSKAQILTSASNVAVCVGDSTQVSAVSGFTGYLW
                     SNGKTTQSIYIKNAGAYTVTVTGSGGCTATASLTLTVNSLPNVSFTNPYQGNGNRACI
                     NDGGVLLNNSGTPGGGYFTGPGVTYGFGLYYFSPGLAGLGLHTLTYTFTDVNGCSSSA
                     TQNVRVDTAATVSAGNDLVKCGNLSVQLSGTIGGSATSATWSTNGTGTFNN"
BASE COUNT          352 a          240 c          218 g          347 t
ORIGIN      
        1 tattattaaa ggttcctgtg ccatttgtac tccaggttgc cgaagttgcg ctgccaccaa
       61 ttgtaccgga aagctgaacg ctcagattgc cacacttaac caaatcatta cctgcactaa
      121 ctgtagctgc agtatcaact cttacatttt gtgtagcact gctcgaacaa ccatttacat
      181 cggtaaatgt ataagttagt gtgtgcaaac ctaaacctgc taaacctggg ctaaaataat
      241 aaaggccaaa gccataagta acaccaggcc ctgtaaaata gccaccgccc ggtgtacctg
      301 agttgtttaa caatacgcca ccgtcattta tacatgctct gttaccattg ccttggtaag
      361 gattggtaaa gcttacgttt ggtaaactgt ttaccgttaa tgttaatgat gcagttgccg
      421 tacagccacc actacctgtt acagttactg tataggcacc ggcatttttt atgtaaatac
      481 tttgcgtggt ttttccatta ctccataagt agcctgtaaa gccacttaca gcacttactt
      541 gcgtactgtc gccaacacat acggcaacat ttgatgcaga tgttaatatc tgagccttac
      601 taaaaactac ttttatagta tggttactgg ctacattatt aaatgtataa ctagccggtg
      661 tgccaataaa tattccattt acaattacgc tatcaatttt tgtacaggta ttgttaggtg
      721 taaaactaaa cgactggctg ccaccttgca ttacatttat tgtacctgat ggagaaatta
      781 cacctgcgcc aactttagaa gcatttattg tgtaactgtt ggttttaaac tgtacatgta
      841 tagtatggtt tacagtaaca ttattaaaag tataggtact gggtgttcct atataattac
      901 catcaacaaa aaccgaatca ataaaataat tggcattggg tgtcatatta aaagtttgcg
      961 acccattgta aacgatatta ataacacctg atggtgatat agcaccgtta gggccggctg
     1021 ttgccgtaat attcaggtta ctggggttac ctgcacgaat aactgcatag gtagggcggt
     1081 aaaacatatt ccaagccaaa ccaccgggta tagtaccatt aacattggca aaagagccaa
     1141 tgcgcgaagc ataagta
//
LOCUS       DAOMAA010000897         1157 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_163856_length_1157_cov_2.225953, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000897 DAOMAA010000000
VERSION     DAOMAA010000897.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163856_length_1157_cov_2.225953"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="PLO59_10570"
     CDS             complement(<1..358)
                     /locus_tag="PLO59_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HQV01595.1"
                     /translation="MFTTLLICVIAFALIFDFINGFHDAANSIATVVSTKVLSPLSAV
                     IWAAFFNFVAYWIFELKVADTVAKTVKPQHIDLVVVLAGLTAAIIWNLITWWQGIPSS
                     SSHTLIGGFAGAGAAHA"
     gene            complement(410..1054)
                     /locus_tag="PLO59_10575"
     CDS             complement(410..1054)
                     /locus_tag="PLO59_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003013112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HQV01596.1"
                     /translation="MALDFLKYFMPQDKKFFPLFEKSAINLALAGTAICDLVKSSDAN
                     ERIRLIREIERLEHVGDEISHEIFQELSRNFITPFDREDIHRLVSAIDNVLDYIHGSS
                     KRMQLYNLTVYSDEMVKIAELLKTQIDELRIAIFELRNMDKMRDISDALVRINSIENH
                     ADDIFDHAVARLFNEETNAVELIKCKEILQALETATDMCEDAANVIESIIIKMA"
BASE COUNT          383 a          223 c          196 g          355 t
ORIGIN      
        1 ctgcatgtgc agcaccggca cccgcaaagc ctccaattag tgtatgcgat gatgatgagg
       61 gtatgccttg ccaccaggta atcaaattcc aaattattgc agcggttaaa ccagctaata
      121 caacaactaa atcaatatgt tgcggcttaa cggttttggc tactgtatcg gccactttta
      181 actcgaatat ccagtatgct acaaaattga aaaaagcagc ccaaattaca gccgataatg
      241 gagataaaac tttagtagaa actacagttg ctattgaatt tgctgcatcg tgaaagccat
      301 ttataaaatc aaaaattaaa gcaaaagcaa taacgcaaat cagtagtgtg gtaaacatgc
      361 aggtttatat ttgaaaattt aggttggtac gagtcaaaac aactaccagc taagccatct
      421 taataataat tgattcgata acattggctg catcttcgca catatcagtt gcagtttcga
      481 gcgcctgtaa tatttcttta cacttaatta attcaactgc attggtttcc tcattaaaca
      541 aacgggcaac ggcatggtca aaaatatcat cagcatggtt ttcaatactg tttatacgta
      601 ccaatgcatc actgatatca cgcattttat ccatgtttcg caattcaaaa atggcaattc
      661 ttaattcatc aatttgtgtt tttaaaagct cggcaatttt aaccatctca tcactgtaaa
      721 cagttaaatt gtatagttgc atacgcttac tgctgccatg tatataatca agcacattgt
      781 caatagccga aaccaaacgg tgtatatctt cacggtcgaa tggtgtaata aaattacggc
      841 ttagctcctg aaaaatttca tggcttattt catcgcccac atgctctaat ctttcaattt
      901 cgcgtattag gcggatacgt tcattagcat cgcttgattt taccaaatcg caaatggcag
      961 tacctgctaa tgcaagattt atggcagatt tttcaaaaag cgggaaaaac tttttatcct
     1021 gaggcataaa gtattttaaa aagtctaagg ccatattttt atgagttaag cgcgcgaaac
     1081 taaaaagtaa tttgtattgc cgcacattta atttatacaa caccggtaaa aatagttagt
     1141 tcaattgcgt atttaat
//
LOCUS       DAOMAA010000898         1155 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_164346_length_1155_cov_2.822727, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000898 DAOMAA010000000
VERSION     DAOMAA010000898.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164346_length_1155_cov_2.822727"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1155)
                     /locus_tag="PLO59_10580"
     CDS             complement(<1..>1155)
                     /locus_tag="PLO59_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01597.1"
                     /translation="ASAFVLWITGFIYSKLEHAFRIKYLILYINLFIALSILAFRAML
                     PFTGSVWFYFAMLVWYNVLYLLNSLDFWGLASLLFDVRQSKRLFGLISAGDIPAKFIG
                     YSSAVIIVPFLGAQNLLVVGAILVLISLRYWYRLNKYGHLNVVVAHAHRNNKAVPGES
                     LKQIVKNFFQNNLVFHMAILSFLIMSTFTVINFTFYAEVKNRLTNNAMLATYIGWFLA
                     FSRGVAIVVKLFFTNRIINYLGITKALLITPVLLSLLFIPVFLMQLSGVGTLKVLLVF
                     GLLSILNDVLRSAIQTPVFLAIMQPLQANVRLRAHNILKGIMDPFAFFFCGVFLYLMI
                     DLEHFVDLQLLCYLLTALLFLSICWILIIDKAYIKALIIAVHNRYLNNKD"
BASE COUNT          434 a          222 c          181 g          318 t
ORIGIN      
        1 cgtccttatt atttaaatac ctgttgtgta cagcaataat taaagccttt atgtatgcct
       61 tatctattat taaaatccaa caaatagaaa gaaacaacaa agctgttaac aggtaacata
      121 gcaattgcaa gtcaacaaaa tgttccaaat ctatcatcag atataaaaac actccgcaga
      181 aaaaaaatgc aaacggatcc ataatgcctt tcagtatgtt atgcgcccta aggcgtacat
      241 ttgcttgcaa tggttgcatt attgctaaaa aaacgggagt ttgtattgcc gaccttaata
      301 catcgtttaa tattgacaga agcccaaaaa ccaatagtac ttttaaagta cctacacctg
      361 acaattgcat taaaaatacg ggtataaaca acagcgacaa taaaacagga gttattagta
      421 atgcctttgt aatacctaag taattaataa tccgatttgt aaaaaacaat ttaaccacaa
      481 tagcaacacc ccggctaaat gccagaaacc aaccaatata tgtagccaac attgcattat
      541 tggtgagcct gttttttact tcggcataaa acgtaaaatt aattacagta aaagtgctca
      601 ttattaaaaa cgataaaata gccatgtgaa aaacaaggtt attttggaaa aagtttttta
      661 ctatttgttt taagctctcg ccaggtaccg ctttgttgtt ccggtgtgca tgcgctacca
      721 ctacattcag atggccgtac ttgttaagcc ggtaccagta tcgtagtgat atcaatacta
      781 atatggcgcc tactaccaat aagttttgtg cacctaaaaa aggaacaatt ataactgccg
      841 atgaatagcc gataaatttt gctggtatat cgcctgcact tattaaacca aacaagcgtt
      901 tgctttgcct aacatcaaaa agcaaggatg caaggcccca aaaatctaaa ctgtttaaaa
      961 ggtatagtac attataccaa accaacatgg caaagtaaaa ccaaacactt ccggtaaaag
     1021 gcaacatggc cctgaatgct aaaatgctta atgcaataaa taaattgatg tagagtatta
     1081 aatatttaat tcgaaaggca tgctccaact tgctataaat aaagccggta atccataaca
     1141 caaaagccga agcta
//
LOCUS       DAOMAA010000899         1155 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_164443_length_1155_cov_2.066364, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000899 DAOMAA010000000
VERSION     DAOMAA010000899.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164443_length_1155_cov_2.066364"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /locus_tag="PLO59_10585"
     CDS             <1..884
                     /locus_tag="PLO59_10585"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HQV01598.1"
                     /translation="SKIVAQSTIRLSATDYINAYAPAAIKDMQLTGVPASITLAQGMF
                     ESDYGNSPLAQQANNHFGIKCHKEWNGKTYIQDDDEKNECFRLYENVQQSYDDHSNFL
                     KTRPRYSFLFSLEQGDYIGWAKGLKQAGYATNPDYANRLIKLIEENNLSQYDKPVLNL
                     QNAPFAHQTDTIIKTSVTVFPLLIPKISYCDDLPYAIAAKGDTWYKLAKENDITLANI
                     FKYNDAGTSTILRQGMLVYLKPKHNCSHIETHLVASGETLWYISQLYGVKLKKLMRYN
                     RIEDAAFLNVGEVIKLR"
     gene            complement(956..>1155)
                     /locus_tag="PLO59_10590"
     CDS             complement(956..>1155)
                     /locus_tag="PLO59_10590"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV01599.1"
                     /translation="NLSDYKNANIHFFDIAGRLVLANDINTTQGFQAIDVSMLEAGIY
                     AYVAIADGQKILQGKVCVISK"
BASE COUNT          395 a          205 c          208 g          347 t
ORIGIN      
        1 gtagtaaaat tgtagctcaa tcaacaatta gacttagtgc aactgattat ataaatgcct
       61 atgcacctgc tgcgattaaa gatatgcaac taaccggtgt gcctgcaagt ataaccttag
      121 cgcaaggaat gtttgaaagt gattatggca actcaccttt ggcacaacaa gctaacaacc
      181 attttggtat taaatgccat aaagaatgga atggtaaaac ctatattcag gatgacgatg
      241 aaaaaaatga atgtttcaga ctatatgaaa atgtgcagca atcttatgat gatcacagta
      301 attttttaaa gacacgtcca cgttacagtt ttttgtttag tttagaacaa ggtgattata
      361 ttggttgggc taaaggctta aaacaagcag gctatgcaac caatcccgat tatgcaaaca
      421 gacttattaa attgattgag gaaaataatt tgtcgcagta cgataagcct gttctcaatt
      481 tacaaaacgc accttttgcg catcaaaccg acacaataat taaaacatcg gttaccgtat
      541 tcccgctatt aattcctaaa atatcgtatt gcgatgatct gccttatgcc attgcagcaa
      601 aaggcgatac ctggtataaa ctggcgaaag agaatgatat aactttggca aacattttta
      661 agtataacga tgccggtacg tcaaccatat tacggcaggg tatgttggta tatttaaagc
      721 caaaacataa ttgctcacat atagaaacgc accttgttgc atcgggcgaa accctttggt
      781 acatatcgca actatatggt gttaaactaa aaaaattgat gcgttataat cgaatcgaag
      841 atgcggcttt cttaaatgtt ggtgaggtta ttaagcttag gtaagttaat tacagaaaca
      901 aaaaagaggc tgtcttaaaa gtaggcagcc tcttttttta tcattaaaaa tcagtttact
      961 tactaattac acaaaccttg ccttgtaaaa tcttttgacc atcggctatt gctacatagg
     1021 cgtaaatacc tgcttccaac atcgaaacat caatagcttg aaaaccttgt gttgtattta
     1081 tgtcgttagc taaaaccaat cttcctgcaa tgtcgaagaa gtgaatattg gcatttttat
     1141 aatcgctcag attat
//
LOCUS       DAOMAA010000900         1154 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_164560_length_1154_cov_3.900819, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000900 DAOMAA010000000
VERSION     DAOMAA010000900.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164560_length_1154_cov_3.900819"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>716)
                     /locus_tag="PLO59_10595"
     CDS             complement(<1..>716)
                     /locus_tag="PLO59_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005863767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HQV01600.1"
                     /translation="FADLVLDATQYIKPIQQNAIVIAQLDCLQSFATTAIAHHYVKPH
                     LNDSFIIDIKAGRHPVLEQQLSIEQTYVANDVYLDNETQQIIIITGPNMAGKSALLRQ
                     TALICLLAQTGSFVPAQQAHLGLVDKIFTRVGASDNISSGESTFMVEMNETASILNNI
                     SARSLILLDEIGRGTSTYDGISIAWAIAEYLHQHHTKAKTLFATHYHELNEMTHTFNR
                     IKNFNVAIKEVENRILFIRK"
     assembly_gap    717..726
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<727..>1154)
                     /locus_tag="PLO59_10600"
     CDS             complement(<727..>1154)
                     /locus_tag="PLO59_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002996930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HQV01601.1"
                     /translation="ADQLNACSILTNQINQTITDEPPAIINKGGTIKNGINEALDALR
                     NIAGNGKAYLVELQKREAESTGIPSLKVSYNHVFGYYIEVTNTHKNKVPTHWHRKQTL
                     ANAERYITPELKQYEEEILGAEEKILGLEESHIKEIFDAI"
BASE COUNT          300 a          198 c          227 g          419 t
ORIGIN      
        1 gtttgcgaat aaataatatt ctgttttcta cttcttttat agctacatta aaatttttaa
       61 tgcgattaaa agtatgtgtc atttcattta attcgtggta atgtgtagca aacaatgttt
      121 ttgctttggt gtgatgttga tgtagatatt cggcaatagc ccaagcaatg cttatgccgt
      181 catatgtaga ggtaccacgc ccaatttcat ctaataaaat caggctgcgg gcactgatat
      241 tatttaaaat gctcgctgtt tcattcattt caaccataaa agttgactcg cctgatgata
      301 tattatcact tgctccaacg cgtgtaaaaa ttttatcaac taagcccagg tgcgcttgtt
      361 gtgccggtac aaaactacct gtttgtgcaa gtaagcatat taacgctgtt tggcgtagta
      421 aggcactttt acctgccatg tttgggcctg taataattat tatttgctgg gtttcattat
      481 ctaaataaac atcgttggca acataagttt gttcaatgga tagttgttgt tcaagcacgg
      541 gatgcctgcc tgcctttata tcaattataa aactgtcgtt taaatgcggt tttacataat
      601 gatgggcaat agctgtagtt gcaaaacttt gtaagcaatc taactgggca ataacaatgg
      661 cgttttgttg tatgggtttt atgtattgtg ttgcatcaag taccaagtcg gcaaatnnnn
      721 nnnnnntgaa tggcgtcgaa gatttcttta atatgacttt cttctaatcc taaaattttt
      781 tcttctgccc ctaaaatttc ttcttcatat tgtttgagtt caggtgtaat gtaccgttcg
      841 gcattggcca gggtttgttt gcgatgccaa tgcgtgggca ccttgttttt atgcgtattg
      901 gttacttcaa tatagtaacc aaaaacatgg ttgtatgata cctttaaaga aggaatgcct
      961 gttgattcgg cttcgcgttt ttgaagctcg actaaatatg ctttgccatt accggctatg
     1021 tttcgcaagg catctaaggc ttcgttaatg ccatttttta tagtaccgcc tttattaata
     1081 attgccggtg gctcatcagt tatcgtttga ttaatttgat tggtaagtat gctacatgca
     1141 tttaactgat cggc
//
LOCUS       DAOMAA010000901         1152 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_165170_length_1152_cov_3.352780, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000901 DAOMAA010000000
VERSION     DAOMAA010000901.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165170_length_1152_cov_3.352780"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1152
                     /locus_tag="PLO59_10605"
     CDS             <1..>1152
                     /locus_tag="PLO59_10605"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - viral entry into host cell
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HQV01602.1"
                     /translation="VIADGTTNVTGCNSKQSSGSTVISYYSDINNATLGANNRAYYAS
                     INATTNTTNTGYQADVLTANGTTASTVTNYGFRTTINGTVQALYSFGFHAQSYKANYN
                     YGIRAEAIPTPGINADALSCGGYFDGGTSNVGTSVINAYGVEAYAHGGSTANYGVAGK
                     CNGSAADNYAVYGEVIQPGAASNNYAGYFAGNVYATGTITSSDQKLKTNITAISNALN
                     IINQLHPKLFDYLSASYPSMHLPTDTHAGLIAQDLEMVLPNLVNTARQPAVTDSNGVT
                     TQAAVDFKGVNYQELIPYLIAAVKEQQQQIDSLANLISNNAYPKQPLQSMSVTLSNQA
                     SIILNQNDPNPFAQNTTITYSIPEEVKNAQLLIYDNNGTILKTITINEK"
BASE COUNT          367 a          279 c          224 g          282 t
ORIGIN      
        1 tggtaattgc cgatggcact accaacgtaa cgggctgcaa ctccaagcaa agcagtggaa
       61 gcacggttat ttcttactac agcgatatta ataacgcaac ccttggcgcc aacaacaggg
      121 cctactatgc cagcataaat gccactacca ataccaccaa cacaggctat caggccgatg
      181 tgcttacggc caacggtacc actgccagta ccgttactaa ttatggtttt agaacaacta
      241 ttaacggtac cgtgcaggca ctgtattcgt ttggctttca tgcacaatcg tataaagcca
      301 attacaacta cggcatcagg gccgaggcca ttccaacacc gggcataaac gcagatgcat
      361 taagttgtgg cggatatttt gatggcggca cttcaaatgt gggtaccagc gtaattaatg
      421 cttatggtgt tgaagcctat gcacatggtg gcagtacagc caattacggt gttgccggca
      481 aatgcaatgg ctccgctgcc gataattatg ctgtttatgg tgaggttata cagcccggtg
      541 cagcttcaaa taattatgca ggttattttg ccggcaatgt gtatgcaacg ggtactatta
      601 ccagttccga ccaaaaactt aaaaccaata ttacagccat tagcaatgcc ttaaacatca
      661 taaaccaatt acaccctaag cttttcgatt acttatcggc aagctatcct tccatgcatt
      721 tgcccacaga cacgcatgcc gggcttatag cacaagattt ggaaatggta ttacccaatt
      781 tggtaaatac tgccaggcag ccagcagtaa ctgatagcaa tggcgtaacc acacaagcag
      841 ccgttgattt taagggggta aattatcagg agttgatacc ctatttaatt gcagccgtaa
      901 aagagcagca acagcaaata gattcgttag ctaatttaat aagcaacaac gcctatccaa
      961 aacagccatt gcaatccatg tctgttacgt taagcaatca ggcatccatc atactcaatc
     1021 aaaacgaccc caatcccttt gcccaaaaca ctaccatcac atattcaata cccgaagaag
     1081 taaaaaacgc acaactgtta atttacgata acaacggtac tatacttaaa accattacga
     1141 ttaacgaaaa ag
//
LOCUS       DAOMAA010000902         1151 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_165513_length_1151_cov_2.354015, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000902 DAOMAA010000000
VERSION     DAOMAA010000902.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165513_length_1151_cov_2.354015"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            44..>1151
                     /locus_tag="PLO59_10610"
     CDS             44..>1151
                     /locus_tag="PLO59_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01603.1"
                     /translation="MKKQYNNHMLLLQKENNASPYKMYLQAELTLQMGLVRAMHNEYV
                     NATVDIRKSYKLFTANNEAFKQFKPNLKGLGFLHILIGAIPENYKWAANLIGLKGTIN
                     QGTAELTELISECVKTDQYDFVKNETLLFLIYVQVHYGKDIPRALQLIKLYDHNYISP
                     YINYSIANVYLMDGKNDNVLHILEDFVPPSDATTLHYLQYLKGMAKTNKLDYSAIDNY
                     KQYIKAYQGLKFKKSACQRIAWLHLIKGDTAQYWQNISQVLQVGSSFTDEDKQAQAEA
                     ETKVIPNITLLKARLLFDGGYYFKSLEQLSQKPATHNVKDNIEAIYRTARNFDKLKQY
                     EKAKTFYNKTISIGDKTTYYFAASAALCLGQMFEE"
BASE COUNT          420 a          207 c          205 g          319 t
ORIGIN      
        1 ttataaatgc tatttaagcg aaagcaagct ggattatgag ctgatgaaga aacagtacaa
       61 caaccacatg ttattactgc aaaaagaaaa taatgcatcg ccctacaaaa tgtacctgca
      121 agccgaactt acactacaaa tggggttggt acgcgccatg cataatgagt atgtaaatgc
      181 aaccgttgat atccgaaaat cgtacaaact ttttactgca aacaatgagg cgtttaagca
      241 atttaaaccc aatttaaagg ggcttggttt tttgcacatt ttaattggtg ccatacccga
      301 aaactataaa tgggctgcca atttaattgg cttaaaaggc accataaacc aaggtactgc
      361 cgaacttacc gagttaataa gcgaatgtgt aaaaactgat caatatgatt ttgtaaaaaa
      421 cgaaacgttg ctatttttaa tttatgtaca ggtgcattac ggcaaagaca taccacgcgc
      481 tttgcagtta ataaaattgt atgaccacaa ttacatatcg ccttatatta attattcaat
      541 agccaatgtt tatttaatgg atggtaaaaa cgataacgta ctgcacatac ttgaagattt
      601 tgtaccgcca agcgatgcaa ctaccctaca ttatttgcaa tatttaaagg gtatggctaa
      661 aaccaataag ctcgattaca gtgccataga caattacaag caatatatta aagcatatca
      721 gggattaaaa tttaaaaaat cggcttgtca gcgtattgcc tggctacact taataaaagg
      781 cgatacagca caatattggc aaaatataag tcaggtgttg caagttggca gtagttttac
      841 cgatgaagac aaacaagcac aagccgaagc cgaaactaaa gttattccca atataacatt
      901 gttgaaagcg cgtttacttt ttgatggcgg ctattatttt aaatcgttag agcaattatc
      961 gcaaaagccc gccacacata atgtaaaaga taatatcgaa gctatttacc gtacggcacg
     1021 caactttgat aaattaaaac aatacgaaaa ggctaaaaca ttttataata aaaccattag
     1081 tataggcgat aaaactacct actattttgc agccagtgca gctttgtgct taggtcagat
     1141 gtttgaagag c
//
LOCUS       DAOMAA010000903         1150 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_165728_length_1150_cov_3.147945, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000903 DAOMAA010000000
VERSION     DAOMAA010000903.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165728_length_1150_cov_3.147945"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="PLO59_10615"
     CDS             <1..586
                     /locus_tag="PLO59_10615"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HQV01604.1"
                     /translation="SLLTLSLHEPFELPTPYKFGNSNEAQLFKSAAYYTDSCLGSFIT
                     AAKQKAWYKNTLIVIVADHGHRLPRQRPFYDPYTHRIPLIITGGALNKNRHGKQVNHT
                     ISQHDVPAFICSQFKMNGTSFKFSKDVMQTQSNFAYLCYDDGLAWVSDSVTFHYNLTQ
                     QKVDFENRIVPDTVLNRAKIYTQQVYTHFLNCGQ"
     gene            complement(587..>1150)
                     /locus_tag="PLO59_10620"
     CDS             complement(587..>1150)
                     /locus_tag="PLO59_10620"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HQV01605.1"
                     /translation="TLLIKQGAVQSVTIIAQQNIADALYTVVQNNRLKIYTDKWLTTD
                     KDIVYEIVVPQITGMELAGSGKFKTTETIKTSKLDITVSGSGQMEVDVVTNELECDIM
                     GSGNIAIKGSSNKTDLEISGSGAYHGFECYTANCDASISGSGLAEVSVSNSLKASVSG
                     SGVINYTGKPKDVKKSISGSGAVNALN"
BASE COUNT          344 a          248 c          185 g          373 t
ORIGIN      
        1 gtctttgtta accttaagct tgcacgaacc atttgaattg cccacacctt ataaatttgg
       61 taatagcaat gaggcgcaac tttttaaaag cgctgcttat tataccgata gctgcttggg
      121 tagttttata actgcagcaa aacaaaaagc ctggtataaa aacactttaa tagttattgt
      181 tgccgatcat ggtcatcggc taccgcgtca acgaccattt tacgatccct atacccatcg
      241 cataccctta attataaccg gaggtgcatt aaataaaaac cggcatggca aacaagtaaa
      301 tcacaccata tcacaacatg atgtgcctgc atttatttgt agtcagttta aaatgaatgg
      361 cacttcgttt aagttttcga aagatgtgat gcaaacacag tcaaactttg catacctgtg
      421 ttatgatgat ggcttggctt gggtttccga tagtgtaacg tttcattaca acttaacgca
      481 acaaaaagta gattttgaaa accgcattgt gcctgacaca gttttaaatc gagcaaaaat
      541 ttacacgcaa caagtttaca cgcacttttt aaattgcggg cagtaactaa ttaagtgcat
      601 ttacagcacc actgccgcta attgattttt ttacatcttt aggtttacct gtataattaa
      661 ttacaccgct acccgatacc gaagctttta aactattgct tacgctaact tcggctaaac
      721 cactgccact aatggatgca tcgcagttag cagtataaca ttcaaatcca tgataggcac
      781 cgctaccgct tatttctaaa tctgttttat tgctgcttcc tttaatagca atgttacccg
      841 aacccataat atcgcattcc aattcatttg taaccacatc aacttccatc tgccccgagc
      901 ccgaaaccgt tatatctaat ttcgatgttt ttatggtttc agtggtttta aacttcccac
      961 tgcctgccag ttccatgcct gttatttgcg gcacaacaat ttcataaaca atatctttat
     1021 cggtagttaa ccatttatca gtataaattt tcagcctgtt attttgtaca actgtatata
     1081 atgcatcggc aatattttgt tgtgcaataa tggtaactga ttgtacggca ccttgtttta
     1141 tcaataaagt
//
LOCUS       DAOMAA010000904         1149 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_166024_length_1149_cov_2.997258, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000904 DAOMAA010000000
VERSION     DAOMAA010000904.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_166024_length_1149_cov_2.997258"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="PLO59_10625"
     CDS             complement(<1..335)
                     /locus_tag="PLO59_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01606.1"
                     /translation="MVVWFENISVNKLNTSYLKQKSLHTKLYEGYYTSDCVIYFLAAF
                     FTTTFLLLAGAGLAATFAITGFASGFLLVAGIAFVSFLAVSATLVTTGFTSVFAAGVT
                     DAGAVFRGVT"
     gene            334..786
                     /locus_tag="PLO59_10630"
     CDS             334..786
                     /locus_tag="PLO59_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018342965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HQV01607.1"
                     /translation="MRLEEAINQKKPFKSNQHKIAVNLVYTYHWLHQQFDKVYKEINI
                     TGQQYNILRILRGQYPNACTVNLLIDRMLDKSSNASRLVDKLVAKTLVKRTVNKSDRR
                     AVDVVITEKGLQFLAETEIKIDALQNKIISLTESESDTLNQLLDKVRG"
     gene            complement(952..>1149)
                     /locus_tag="PLO59_10635"
     CDS             complement(952..>1149)
                     /locus_tag="PLO59_10635"
                     /inference="COORDINATES: protein motif:HMM:NF025274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease C-terminal domain-containing
                     protein"
                     /protein_id="HQV01608.1"
                     /translation="VKSPEIPRPFRAPAAPFVSVMGVLVCTAMIVALDHRTLKAAFAW
                     MLIGIVVYMLYGRHHSKLKKS"
BASE COUNT          416 a          218 c          212 g          303 t
ORIGIN      
        1 gtaacacccc tgaaaacagc accggcatca gttacaccgg ctgcgaaaac agaagtaaag
       61 cctgtggtaa ctaaagtggc cgaaacagct aaaaaggaaa caaaagcaat accggcaacc
      121 aataaaaaac ctgaggcgaa acccgttatt gcaaaagttg ctgctaagcc tgcacccgct
      181 aacaacaaaa aagtggtggt aaaaaaggcg gctaaaaaat agatgacgca atcagaagta
      241 taatagcctt catacaattt tgtgtggagg cttttttgtt ttaaataaga tgtgtttaat
      301 ttgtttacac taatattttc aaaccatact accatgcgtt tagaagaagc cattaatcag
      361 aaaaaaccat ttaaaagcaa ccaacataaa atagctgtaa acttagttta tacttaccat
      421 tggctacacc agcagtttga taaggtatat aaagaaataa atattaccgg acagcaatac
      481 aatatactac gcattttgcg tggccaatat cctaacgcat gtacggtaaa cttactaatt
      541 gaccgtatgc ttgataaaag cagcaatgca tcgcgtttgg tagataagct ggtggctaaa
      601 accttagtaa agcgcacagt taataagagc gaccgtagag cagttgatgt agtgataact
      661 gaaaaaggtt tgcagttttt agccgaaaca gagattaaaa tagatgcact tcaaaataaa
      721 attatatcat taaccgaaag cgaatcagat acattaaacc aacttttgga taaggtgcgt
      781 ggttagtcgt taggatacaa ttaacctgat ttaatttaat acgctccata aacattttag
      841 ttaggcagat aaaaaaaggc cgctgtaaat aattacagcg gccttttttt atgtatgctt
      901 aagctgcttt aattgcctaa gcgctttttt aatagtagtg aaacagaagc actacgattt
      961 ttttaacttg ctgtgatgcc ttccatacaa catgtaaact acaataccaa tcagcatcca
     1021 ggcaaaagct gcctttaatg tgcgatgatc taaggcaact atcatagccg tacaaactaa
     1081 cacgcccatt acactaacaa atggtgcagc aggtgcccta aacggacgtg gtatttccgg
     1141 actttttac
//
LOCUS       DAOMAA010000905         1149 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_166084_length_1149_cov_2.470750, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000905 DAOMAA010000000
VERSION     DAOMAA010000905.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_166084_length_1149_cov_2.470750"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="PLO59_10640"
     CDS             <1..401
                     /locus_tag="PLO59_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HQV01609.1"
                     /translation="RTKDIFNVVLGVIAGISLLVGGIGIMNIMLASVLERIKEIGIRL
                     SMGATKVDIVQQFLYEAVLISITGGVAGIILGIALSLIIGQVAQIQTIISVWSVIISF
                     GVAALTGLIFGITPAKRAAMLDPIQSLRHE"
     gene            453..929
                     /locus_tag="PLO59_10645"
     CDS             453..929
                     /locus_tag="PLO59_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01610.1"
                     /translation="MNNKNLLIIICLFAYSYLNAQTGALNFTLQDVIQMANAQSPQAK
                     IAKTTLENKYWQFRTYKSNYLPQLALNGTIPDFNKSNNAITQPDGSIVFRNQSFSTSS
                     LEVSLSQNIGPTGGQIFASSYLQRLDALSTPTNISYLSNPVLIGFRQPLFRFNQLK"
     gene            948..>1149
                     /locus_tag="PLO59_10650"
     CDS             948..>1149
                     /locus_tag="PLO59_10650"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQV01611.1"
                     /translation="MLYEESKRQYAEDLEAVSVLASERFFNLLIAEIRVRIESLNLAN
                     NDTLFQIAKGRYNLGRIAENELL"
BASE COUNT          358 a          218 c          214 g          359 t
ORIGIN      
        1 aacgcacaaa agatattttt aatgtggtgt tgggtgtaat agcaggcata tccttgttag
       61 tgggcggtat tggtatcatg aatattatgc tggcatcggt gttggaacga attaaagaaa
      121 ttggcattcg cttatcaatg ggtgctacca aggttgatat tgtgcaacaa tttttatacg
      181 aagctgtttt aattagcatt accggaggtg ttgccggtat aatattaggc attgccttaa
      241 gtttaattat agggcaggtt gcacaaatac aaactattat ttcggtttgg tcggtaatta
      301 tatcatttgg cgttgccgca cttaccggat tgatatttgg cataacgcct gctaaacgcg
      361 cagctatgtt agatcccatt caatcattaa gacatgaata gttttatatt ttaatcacta
      421 tcaacccatt attgtttttg gctcttaaaa tcatgaataa caaaaacctg cttatcataa
      481 tttgtttatt tgcttatagt tacctaaatg cccaaaccgg tgcattaaat tttacattgc
      541 aagatgtaat acaaatggca aatgcacaat cgccacaggc aaaaattgcc aaaaccactt
      601 tagaaaataa gtattggcag tttcgcacct acaaatcaaa ttacctgcca cagttggctt
      661 taaacggcac aattcctgac tttaataaat caaacaatgc cattacacaa cccgatggca
      721 gtattgtatt tcgtaaccaa tctttttcaa catcatcgtt agaagtatcg ttatcacaaa
      781 acatcggacc aacaggcggc cagatttttg cctcatccta tttgcaaaga ttagatgcat
      841 taagtacacc caccaatatc agttatttat ccaatccggt tttaatagga ttcaggcagc
      901 cattattccg gtttaatcag ttaaaatgag atgcgcgcat acagcccttg ttatatgaag
      961 aatcgaaacg gcaatatgcc gaagatttag aagctgtttc ggtattggca tcggagcgtt
     1021 tttttaattt gttgatagct gaaatacgcg tacgcatcga atcgttaaac ttggccaaca
     1081 acgataccct gtttcaaata gccaaaggcc gatataactt aggtcgcatt gccgaaaacg
     1141 aattgttgc
//
LOCUS       DAOMAA010000906         1147 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_166570_length_1147_cov_3.680403, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000906 DAOMAA010000000
VERSION     DAOMAA010000906.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_166570_length_1147_cov_3.680403"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..1006
                     /gene="mreC"
                     /locus_tag="PLO59_10655"
     CDS             164..1006
                     /gene="mreC"
                     /locus_tag="PLO59_10655"
                     /inference="COORDINATES: protein motif:HMM:NF010532.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HQV01612.1"
                     /translation="MRNLIQLLWKYNFLLLFILLQVFCFYRIIDEQKFHKAAVLNSSN
                     AMVANAMQKVNEVKDYLWLRENNAYLAAENARLKAYDSTLFFDLSTHKSGTVDSAYAI
                     QYQFIVAKVVNNTIHNRNNFITVNKGFANGIEPDMGVISASGAVGVVKDVSEHFSTIQ
                     SLLHSKTRASVKLKSGFYGPLTWDGSHPQRATLSEIPANVKVNVGDTVVTTAFSTIFP
                     EGVMVGRVLSVKPGSGGDFLKITVALSTNFSNLSHVYIVTNKFKIEQKTLEAKTDSID
                     KNGN"
BASE COUNT          385 a          204 c          216 g          342 t
ORIGIN      
        1 ttattaaaaa tgatagcttg ttaaaaaact acttcataac tcatttgtca gttatataag
       61 acaacctttt gtagattaat tttaacccca agctttatta aaaaaataat aacgcgtaaa
      121 aaaaatctgc tcccatttaa atacttaagc tgcaacagcc accgtgcgta atttaattca
      181 gttactttgg aaatacaatt tcttgttgct gtttatactg cttcaggtgt tttgttttta
      241 tcgcattatt gacgagcaaa aatttcataa agcagccgtg cttaattcgt caaatgcaat
      301 ggtggctaat gccatgcaaa aagtaaatga agtaaaagac tacttgtggt tgcgcgaaaa
      361 caatgcatac ctggctgccg aaaatgcacg actaaaagca tacgacagta cgctgttttt
      421 tgatttaagt acgcataaat caggcacggt tgattcggcc tatgccatac aataccaatt
      481 tattgtagcc aaagtggtta ataacaccat ccataaccgt aataatttta ttaccgttaa
      541 taaaggcttt gcaaatggta tcgaaccgga tatgggcgta ataagtgcat ccggtgccgt
      601 aggtgtggta aaagatgtat cggaacattt tagcaccata caatcattat tacattcaaa
      661 aacaagggcc agtgtaaaac taaaatcagg gttttatggt ccgctaacct gggatggtag
      721 ccatccgcaa cgcgccacac taagcgaaat accggccaac gtaaaagtaa atgtgggcga
      781 tacggttgta acaactgctt tctcaaccat ttttcctgaa ggggtaatgg taggtcgtgt
      841 actgtcggtt aaaccgggca gcggaggcga ttttttaaaa attacagttg ctttaagtac
      901 caatttttca aacctcagtc atgtttatat tgttaccaat aaatttaaaa ttgagcaaaa
      961 aacattagaa gcaaaaaccg actcaataga taaaaatggc aattgaagta attaaaataa
     1021 tactcaggtt tttgttgctg attggagtgc aggtggctgt gcttaatcat atcgaactaa
     1081 caggcttaat aaatccgtat ttatatgtaa tggccttact tgcactgccg tttaagatgc
     1141 taaaagg
//
LOCUS       DAOMAA010000907         1147 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_166598_length_1147_cov_3.088828, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000907 DAOMAA010000000
VERSION     DAOMAA010000907.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_166598_length_1147_cov_3.088828"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="PLO59_10660"
     CDS             <1..485
                     /locus_tag="PLO59_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01613.1"
                     /translation="FLKNKTHNIQVQFIGVASQQAMLQKINAFIPQPFLITTPMLDKQ
                     EALQYTKNADVLLQFGWTDYNGIVGTKTFDYMASGNPILLAPADVVMQQLFDKTNAGI
                     TVSTTHLFVQALETWYDEWKQNGHIKWHTHLGVVETYSRKNQSNLMAALIFETLNTPA
                     "
     gene            486..>1147
                     /locus_tag="PLO59_10665"
     CDS             486..>1147
                     /locus_tag="PLO59_10665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQV01614.1"
                     /translation="MHIVVVFSNIENSLAFYWMAEAFAKLPHQFTYVFMHPHKPALAT
                     QIEALGNRVIQIKYAGKNDGLTAGMQLYSLFKKLKPDVVHAHLFDASMFALTAAWLAG
                     IKKRVYTRHHSDFHHKSYKHAIKYDRWINNRATHIVAISENVKEILIKLEHVKPTKIY
                     LIHHGFNLDALVQVEAEAVDALKLKYNAKQQYPVIGVISRFTYWKGIQYIIPAFEQLL
                     QQ"
BASE COUNT          401 a          218 c          211 g          317 t
ORIGIN      
        1 tttttttaaa aaataaaacc cataatattc aggtgcagtt tattggtgtg gcttcgcagc
       61 aagcaatgtt gcaaaaaata aatgctttta taccacaacc atttttaata actacaccta
      121 tgctcgacaa gcaggaagca ttacaataca ccaaaaatgc cgatgtatta ttgcaatttg
      181 gttggacgga ttataacggc atagtaggca ctaaaacttt tgactatatg gcatcgggca
      241 atccgatttt gttggcacct gccgatgttg taatgcaaca acttttcgat aaaaccaatg
      301 ccggtataac tgtaagcacc acacatttat ttgtgcaagc attagaaacc tggtacgatg
      361 aatggaaaca aaacggccat attaaatggc atacccactt gggtgttgtt gaaacttaca
      421 gccgtaaaaa ccaaagcaat ctaatggcag cacttatttt tgaaacttta aatacacctg
      481 cataaatgca tatagtagta gtgttttcaa atatcgaaaa cagcttagcc ttttactgga
      541 tggcagaagc gtttgcaaaa ctgccacatc agtttaccta tgtttttatg catccgcata
      601 aaccggcact tgcaacgcaa atagaggctt tgggtaatcg ggtaatacaa ataaaatatg
      661 caggcaaaaa cgatggacta acagcaggca tgcaactata tagtcttttt aaaaagttga
      721 aacccgatgt agtgcatgcc catttgtttg atgcttcgat gtttgcactt actgcggcat
      781 ggctggcagg cataaagaaa agagtttata cacggcacca cagtgatttc caccacaaat
      841 catacaaaca tgctataaag tatgatagat ggataaataa cagagccaca catatagttg
      901 ctatttctga aaacgtaaaa gagatactta taaagttgga acatgtaaaa ccaacaaaaa
      961 tttacttaat acatcatggc tttaatctgg atgcacttgt gcaagtagaa gccgaagcag
     1021 ttgatgcgtt aaaactgaag tacaatgcaa aacagcaata tccggtaatt ggtgttatat
     1081 cgcgttttac ttactggaaa ggcattcaat acatcatacc cgcttttgaa caactactgc
     1141 aacaaca
//
LOCUS       DAOMAA010000908         1146 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_166915_length_1146_cov_2.870761, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000908 DAOMAA010000000
VERSION     DAOMAA010000908.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_166915_length_1146_cov_2.870761"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="PLO59_10670"
     CDS             complement(<1..188)
                     /locus_tag="PLO59_10670"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQV01615.1"
                     /translation="MSNNYFDNKIILITGASSGIGRALSLELAAPNVHLFIASRNMEA
                     LQHVKQQCINKGAMCTVF"
     gene            533..>1146
                     /locus_tag="PLO59_10675"
     CDS             533..>1146
                     /locus_tag="PLO59_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01616.1"
                     /translation="MRFSLVLIFFITGLKTAWAQVQFVKEYIPNTIGKQINVLPSANG
                     FKIFTLDSFKLSNFDLCGNSVLCKKYFLPVSATVLGDVKQLANGDLVVLSRKDYINTS
                     GMVLTRISDNGNVIWSFEYGDNIQSLFPYTVSEDQMGNLYVFMNAENSVTNQVQSCVL
                     KTDNTGNIIWSNFYGYGIIWGGGITTNDGGFLFRTGNTLIKLDG"
BASE COUNT          366 a          171 c          207 g          402 t
ORIGIN      
        1 aaaaaaacag tgcacattgc acctttattt atacactgtt gcttaacatg ctgtaaagct
       61 tccatatttc gtgaagctat aaacaaatgc acatttggtg cagccaactc taatgataat
      121 gcccgcccta tgccagaaga ggcacctgta attaaaatga ttttattgtc gaaataattg
      181 ttgctcaatt ttgaaaagca ttattgatgt tgcatagata aaaagtattt tgacaataaa
      241 gacgctcatt tgttcacagt taatagctaa cgcacattaa taactattac ctcgaatctt
      301 tttgaaatta aaattttgca agcgtttgcc gtttatattt ttaaagcaac caaaaacacc
      361 ttgtgtattg taattgtatt cgagcccagg ttttaattgc aagatttatt ttaaccgttt
      421 taaattgtat ggctttataa cttgttaagt tacaattata aggtttaagg cttcgtgtaa
      481 atttttaagt ttgattattt tcggcctttc gaatagttga acatttacaa caatgcgatt
      541 ttcattggta cttatatttt ttattaccgg cttaaaaaca gcatgggcac aagtgcagtt
      601 tgtaaaggag tatataccta acacaatagg caaacaaatt aatgttttac cttctgctaa
      661 tggttttaaa atttttacac tcgatagttt taagctcagt aattttgatt tgtgtggcaa
      721 tagtgtgctt tgcaaaaaat actttttgcc cgtatcagca acggttttgg gcgatgtaaa
      781 acaacttgcc aatggcgact tagttgtttt aagccgaaaa gattatataa atacttcagg
      841 catggtgctt acacgcataa gcgataatgg caatgtaatt tggagttttg agtatggtga
      901 taatatccaa tcgctgtttc cttatactgt aagtgaggat cagatgggta acttatacgt
      961 ttttatgaat gctgaaaaca gtgttaccaa ccaggtgcaa agttgtgttt taaaaactga
     1021 caataccggc aatattatct ggagcaattt ttacggttat ggtattatat ggggaggcgg
     1081 cataacaact aatgatggtg gttttttatt tagaaccggc aatacattaa ttaaattaga
     1141 tggtaa
//
LOCUS       DAOMAA010000909         1145 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_167171_length_1145_cov_2.943119, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000909 DAOMAA010000000
VERSION     DAOMAA010000909.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_167171_length_1145_cov_2.943119"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="PLO59_10680"
     CDS             complement(<1..835)
                     /locus_tag="PLO59_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002808935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HQV01617.1"
                     /translation="MELLRFSTAGSVDDGKSTLIGRLLYDSKSIFEDQMDAIKSYSDK
                     KGYEHVDLALLTDGLRAEREQGITIDVAYRYFATPKRKFIIADTPGHIQYTRNMVTGS
                     SNSNLSIILIDARNGIIEQTNRHSFIASLLNIPHLVVCVNKMDLVDYSEARFNEIVEG
                     YKILASKLDIKDVHFIPISALKGDNVVDRSETMKWYNGPTLMYLLENVHISSDLNHID
                     CRFPVQYVIRPQAQEYHDYRGFAGRVASGIYKPGDRVLCLPSGFTTSIKSIDTFEGPV
                     EE"
     gene            complement(892..>1145)
                     /locus_tag="PLO59_10685"
     CDS             complement(892..>1145)
                     /locus_tag="PLO59_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020778094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfate adenylyltransferase small subunit"
                     /protein_id="HQV01618.1"
                     /translation="RDGMILSTSDFIPLRDGEKPEMKTVRYRTVGDMTCTGAVYSDAS
                     DIDMIIDEVSVSRTTERGTRADDKRTEAAMEDRKKAGYF"
BASE COUNT          325 a          232 c          236 g          352 t
ORIGIN      
        1 cttcttcaac cgggccttca aaagtatcta ttgatttaat ggatgttgta aagcctgatg
       61 gcaagcacaa taccctatcg cccggcttat aaataccgga tgcaacacga cctgcaaagc
      121 cacggtagtc atgatactcc tgtgcttggg gacgtattac atattgtacc ggaaaacggc
      181 aatcaatatg atttaggtcg gacgatatgt gtacattttc aagtaagtac attaaagttg
      241 ggccattgta ccatttcatg gtttccgaac ggtcaacaac gttatcgcct tttaatgcgc
      301 taatcggaat aaagtgtaca tccttaatat ctaatttaga ggcaagtatt ttatagcctt
      361 caacaatttc gttgaagcgt gcttcgctat agtcaaccaa atccattttg tttacacata
      421 ctaccaagtg cggtatgtta agcaaactgg caataaatga gtggcggttg gtttgttcga
      481 taataccgtt gcgggcatca attaaaataa ttgataagtt agagttgctg ctaccagtaa
      541 ccatgttgcg cgtgtactga atatggcccg gtgtatcggc aataataaat ttacgcttag
      601 gcgttgcaaa atagcggtaa gccacatcaa tggttatacc ttgctcgcgc tcggcacgca
      661 aaccatcggt aagtaaagcc aagtcaacat gctcataacc ttttttgtcg ctgtatgatt
      721 taatggcatc catttgatct tcgaaaattg atttcgagtc ataaagcaaa cgtccgatta
      781 atgtgctttt tccatcgtca acactacctg ccgtagaaaa tcgtaataat tccatgattt
      841 atattttgtt ttttttattg ataaattgaa gtgattgggg agtgagtttg attaaaagta
      901 accggctttt ttacggtctt ccattgcagc ttcggtacgt ttatcgtcag ctcgggtgcc
      961 gcgctcagtt gtacgcgata ccgatacttc gtcaataatc atatcaatgt cagaagcatc
     1021 agaatataca gcaccggtac aggtcatatc gccaacggta cggtagcgta cggttttcat
     1081 ttcaggtttt tcgccatcac gtaatggaat aaaatcgctg gtagaaagta tcataccatc
     1141 gcgtt
//
LOCUS       DAOMAA010000910         1144 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_167454_length_1144_cov_2.742883, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000910 DAOMAA010000000
VERSION     DAOMAA010000910.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_167454_length_1144_cov_2.742883"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /locus_tag="PLO59_10690"
     CDS             <1..757
                     /locus_tag="PLO59_10690"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV01619.1"
                     /translation="HLGAIHRLHQSFCQDINIADVDHNGYDDILFVAQYPNGIFGTIN
                     KFTLLRNNTVATTLNIHHKFPSGGECFPNNAIRNIQWLSSVPAGNQSSVQIAFSETGN
                     TGPWTTIANAAPNNGNFQWAVPAGITSSNCYIRLIVTDSTTLLTNTSYNSNAFQVGVC
                     DPTLALSETASSVSFYAFPNPFMSSLNIKSVTPISSVLIVDPTGREIYSQINIPEPKQ
                     LLQINTDTFAKGVYFVKATSVSGAVLTHKVTKY"
     gene            910..>1144
                     /locus_tag="PLO59_10695"
     CDS             910..>1144
                     /locus_tag="PLO59_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HQV01620.1"
                     /translation="MKKTNNLIFTGLQIVAWIIFIGLCIEAGGLLVNFVFSIYNPDMI
                     SRLYNKLNLMAMYQQSAFAFYAVYSFILIIAILK"
BASE COUNT          345 a          239 c          190 g          370 t
ORIGIN      
        1 gcaccttggg gccatccacc gcctccacca aagtttttgc caggatatta atattgccga
       61 tgtagatcat aatgggtatg acgatatttt atttgtggcc caatacccga acggtatttt
      121 cggtaccatc aataagttta cactgctcag aaataacacc gttgcaacaa ccctaaatat
      181 tcatcataaa ttcccttccg gtggcgagtg ttttcccaat aacgccatca gaaatattca
      241 atggctaagc agtgtgcctg caggcaacca atcttcagta caaattgcat tttcagaaac
      301 aggtaacaca ggcccatgga ctaccatagc caatgcagcg cctaacaacg gcaattttca
      361 atgggctgtg cctgccggta taacttcaag caattgttac atacgtttaa tagttactga
      421 cagtactact ttattaacca acacttcgta taacagcaat gcgtttcagg ttggtgtttg
      481 cgatccaaca cttgcattga gcgaaacagc atcatccgtt agcttttatg cttttccaaa
      541 tccatttatg tcgagtttaa acattaagtc tgttacaccc atttcatctg tattaatagt
      601 tgacccaacc ggccgtgaga tatacagcca aattaatata cccgaaccaa agcaattgtt
      661 acaaataaat accgatactt ttgccaaagg cgtttacttc gtaaaggcaa catcggtaag
      721 tggcgctgtt ttaactcata aagtaaccaa gtattaaagg catccaatag ctttttctca
      781 tttatatttt attaaccagc ggttgctgtt ttttacacat ggcaaatagt taatgttaaa
      841 taatatatat ttattgtttt tcaataaata tatattatgt ttgtgccctc agtcatacat
      901 aaatatatta tgaaaaaaac aaacaacctc atttttacgg gtttacaaat tgtggcctgg
      961 attattttta ttggcttgtg tattgaagcc ggtggcttat tggtcaactt tgttttcagt
     1021 atttacaatc ccgacatgat ttcgcggctt tacaacaagc ttaatttgat ggccatgtat
     1081 caacaaagcg cctttgcctt ttatgctgta tattccttta tactgataat tgccatacta
     1141 aaag
//
LOCUS       DAOMAA010000911         1142 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_167983_length_1142_cov_3.072677, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000911 DAOMAA010000000
VERSION     DAOMAA010000911.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_167983_length_1142_cov_3.072677"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1142
                     /gene="serS"
                     /locus_tag="PLO59_10700"
     CDS             <1..>1142
                     /gene="serS"
                     /locus_tag="PLO59_10700"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008239650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HQV01621.1"
                     /translation="ILTIDDERRKSQKTSDDLKANQNQLAKQIGDLMKQGKKAEAELL
                     KSEVAQLKEQSKNMDDEIAALMANIDRLVLTMPNIPGSSVPAGRTAQDNLTVYKNYTD
                     LPVLPAHAMPHWDLAAKYRLIDWELGVKITGAGFPVYIGKGARLQRALINYFLDKALA
                     AGYTEIQPPIVVNEQSGFGTGQLPDKEGQMYFVNEDGFYLIPTAEVPITNIYRDHIFK
                     ADELPVKMMGYTPCFRREAGSYGKDVRGLNRLHQFDKVEIVQFQTPEKSWETLEEMRH
                     YVAGLLHELGLPFRTLLLCGGDMSFASSMTYDMEVYAAAQQRWLEVSSVSNFQSFQAI
                     RLNCRLKAEDGTNYVHTLNGSALALPRIVASILENNQTPDGIKIPE"
BASE COUNT          367 a          228 c          257 g          290 t
ORIGIN      
        1 gattttaacc atagatgatg aacgcagaaa aagccaaaaa acatctgacg atttaaaagc
       61 aaatcaaaat caattagcca agcagatagg cgatttaatg aagcaaggca aaaaggccga
      121 agccgaattg ttgaaatcag aagtagcaca gctaaaagaa caaagcaaaa atatggacga
      181 tgaaattgct gcattaatgg caaacattga caggttggta ctaaccatgc ccaatatacc
      241 cggcagcagc gtgccagccg gccgaactgc acaggataat cttaccgtat ataaaaacta
      301 taccgacttg ccggtattac ccgcacatgc aatgccacat tgggatttag ctgctaaata
      361 ccgcttaatt gattgggaat tgggcgtaaa aattaccggt gcaggttttc cggtttatat
      421 tggcaaaggt gcccggttgc aacgtgcatt aattaattat tttttagata aggcactggc
      481 tgcgggctac acagaaatac aaccacctat tgtggtaaat gaacaatcgg gttttggcac
      541 cggtcaactg cccgataaag aaggacaaat gtattttgta aacgaagatg gattttattt
      601 gataccaacg gccgaagtac ccattaccaa tatttaccgc gaccatattt ttaaagccga
      661 tgaattaccc gtaaaaatga tgggttacac accttgtttc agacgcgaag cgggtagcta
      721 tggtaaagat gtgcgcggcc ttaaccgatt gcaccaattt gataaggtag aaattgtaca
      781 atttcaaacg cccgaaaaat cgtgggaaac gttagaagaa atgcgccact acgtagcagg
      841 tttactgcat gagttgggct tgccttttcg tacgttgttg ttatgcggag gcgatatgag
      901 ttttgcatcg tcaatgactt atgatatgga agtttacgca gctgcacagc aacgctggtt
      961 agaggtaagc tcggtttcga atttccagtc gtttcaggcc atcagattaa actgccgctt
     1021 aaaagccgaa gatggtacca actatgtgca tacacttaat ggtagtgcat tggccttgcc
     1081 gcgcattgtg gcttctattt tagaaaacaa tcaaacaccc gatggtatta aaatacccga
     1141 ag
//
LOCUS       DAOMAA010000912         1141 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_168462_length_1141_cov_1.641805, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000912 DAOMAA010000000
VERSION     DAOMAA010000912.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_168462_length_1141_cov_1.641805"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..405)
                     /locus_tag="PLO59_10705"
     CDS             complement(40..405)
                     /locus_tag="PLO59_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01622.1"
                     /translation="MDKDKILQCNQIIAQFMGWDAEELRIYMTLKAPSMGKVAYNESW
                     DLLMPVIEKIESLPTNKEEGEEYQFSITGDGVAITQYDDGSGVIAQRINEIGKSKLMS
                     AFEVVAAFCCDYFKIERRS"
     gene            complement(522..1007)
                     /locus_tag="PLO59_10710"
     CDS             complement(522..1007)
                     /locus_tag="PLO59_10710"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HQV01623.1"
                     /translation="MKNIYKILAAVGLLLLIPANINYPRAFFKGKKNKLRACDAGGCG
                     YFGAPRKDHIHKGVDVVCTPGTTVISPVDGIITRSFLPYANDNNYNGVEIATVDNKIK
                     IMYMLPYFEKIGTTVKAGEPIGVAQDISKKYYSAVTPHLHVELWSDGQPINGASFFNI
                     V"
BASE COUNT          286 a          252 c          192 g          411 t
ORIGIN      
        1 tccgccacat cgtatagcac cgatacgtta tcggcaaccc tatgaccgcc tttcgatttt
       61 aaaatagtcg caacaaaaag cagctaccac ttcaaaggct gacatcaatt tgcttttacc
      121 tatctcgtta attcgttgtg caatcacacc actaccatca tcatattggg ttattgctac
      181 cccatcacca gtaattgaaa attgatattc ttcaccttct tctttgttgg ttggtaggct
      241 ttcaatcttt tcaattacag gcataagcaa atcccaactt tcattgtatg ctaccttgcc
      301 catgcttggt gcctttagcg tcatataaat tctcaactct tctgcatccc aacccatgaa
      361 ttgtgcgatt atttgattgc actgtaaaat tttgtctttg tccattttat gtttgttttt
      421 aggttaataa tccgggaaag ggcagccgat aaccgccgtt tggcgcaata gccccaacgc
      481 tgcaagccga cccttcggct acttcgccag gctgcctacc gttatactat attaaaaaaa
      541 cttgctccat taattggctg tccatcgctc cataactcca catgtaaatg tggtgttact
      601 gctgaataat attttttgct tatatcctgt gctactccaa ttggttcccc ggcttttact
      661 gtggtgccta ttttctcgaa gtatggcaac atgtacatga tttttatttt attatccacc
      721 gttgctattt ctactccgtt ataattgtta tcgtttgcat aaggtaaaaa tgaacgtgtt
      781 ataattccat ctactgggct aattactgtt gtgccgggtg tacatacaac atcaaccccc
      841 ttatgtatgt ggtctttacg tggtgcgcca aaataaccac aacccccggc atcacatgct
      901 cttagtttat tttttttgcc tttaaaaaaa gctcgtgggt agtttatgtt tgcaggtatt
      961 aatagcaata aacctactgc tgctaaaatt ttatatatgt ttttcattta tccaaaaatt
     1021 gattttatta cctgctgctc tgccggtgtt attagtttgt atttgaatgt gatgttgtgg
     1081 agctgcaacc aattatataa tgtgtttcgg ctaacttcgt atgttgatga taattgcttt
     1141 t
//
LOCUS       DAOMAA010000913         1136 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_169718_length_1136_cov_3.319149, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000913 DAOMAA010000000
VERSION     DAOMAA010000913.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_169718_length_1136_cov_3.319149"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1136
                     /gene="feoB"
                     /locus_tag="PLO59_10715"
     CDS             <1..>1136
                     /gene="feoB"
                     /locus_tag="PLO59_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HQV01624.1"
                     /translation="FQTIFTLAQYPMDFFDNWFSAFSIWVADNLPAGLLNNLLVDGVL
                     AGLGGILIFIPQIALLFAFIALLEDSGYMARVSFIMDKLMRGIGLNGRSVIPLIGGVA
                     CAVPAIMGTRTIQNWRERMVTIFVLPLISCSARLPVYALLIALVIPPVKWFGFFNLQG
                     LVLLVMYLIGFAAVVISALVIKLFIKDKQPSYFIMELPVYRVPQWSNVLYMVWDKVKV
                     FVFDAGKIIIAISIILWVLSSFGPSLKMENIDKQFALQLAQPQADTAAVTNTMHAHKL
                     EHSYAGIMGKKLEPLIAPLGFDWKIGIALITSFAAREVFVGTMSTIYSVGTDNMLSVK
                     AKMQAEKNRHTQQPFYNMAVGVSLMLFYVFAMQCMSTLAIVKRE"
BASE COUNT          298 a          194 c          247 g          397 t
ORIGIN      
        1 gtttcaaacc atttttacac ttgcccaata tccaatggat ttttttgaca attggttttc
       61 ggcatttagc atttgggttg ccgataattt gccggcaggt ttattaaaca atttattggt
      121 tgatggtgta cttgcaggcc tgggcggcat actcattttt attccgcaaa tagctttgct
      181 atttgctttt atagccttac tcgaagatag tgggtatatg gcccgcgtta gctttataat
      241 ggataaactg atgcgcggta ttggattaaa tggccgtagt gtaataccac ttattggagg
      301 tgttgcctgt gctgtgccgg caataatggg cacacgcacc atacaaaact ggcgcgagcg
      361 catggttact atttttgttt tgcctctgat aagttgctcg gcaaggttgc cggtttatgc
      421 attgttaatt gcattagtaa ttccacctgt taaatggttt ggctttttta atctgcaggg
      481 tttggtgctt ttggttatgt atcttatcgg atttgctgct gttgttatat cagctcttgt
      541 tattaagctt tttattaaag ataaacagcc aagttatttt ataatggagt tgcctgttta
      601 ccgtgtgcca caatggtcga atgtgctata tatggtatgg gataaggtta aagtgtttgt
      661 atttgatgcc ggtaaaataa ttatagcaat ttcaattata ctttgggttt tgtcatcgtt
      721 tgggccttca ttaaaaatgg aaaacattga taagcaattt gcattgcaat tggcacaacc
      781 acaagccgat acagccgctg ttaccaatac aatgcatgcg cataaactcg aacattctta
      841 tgcaggcatt atgggcaaaa agttagaacc acttattgca cctttaggtt tcgattggaa
      901 aattggtatt gcattaatta catcgtttgc cgcacgcgaa gtttttgttg gtaccatgtc
      961 aactatttac agcgttggta ccgataatat gctgagtgta aaagcaaaaa tgcaagccga
     1021 aaaaaaccgg catacacaac aaccttttta taatatggct gtaggtgttt cgctcatgtt
     1081 gttttatgta tttgccatgc agtgtatgag cacattagcc attgttaagc gtgaaa
//
LOCUS       DAOMAA010000914         1133 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_170571_length_1133_cov_3.730983, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000914 DAOMAA010000000
VERSION     DAOMAA010000914.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_170571_length_1133_cov_3.730983"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="PLO59_10720"
     CDS             complement(<1..563)
                     /locus_tag="PLO59_10720"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HQV01625.1"
                     /translation="MQYRAVHFSAEVYPVAKTGGLGDVAGALPKYLNDAGCSTCVIMP
                     RYNSKWDAANNTVPVHHGYMKSEHYGVDYMIRKSMVDFGFDLYFVDLPYLLYRENLYG
                     YSDDPERFLFFQHAALDWINTWAELPEILHCHDHHTGLIPFMVEHCYKFERMRGIKSV
                     FTIHNALYTGACRWSMARYFPDFSYHL"
     gene            complement(928..>1133)
                     /locus_tag="PLO59_10725"
     CDS             complement(928..>1133)
                     /locus_tag="PLO59_10725"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV01626.1"
                     /translation="ASNQLTIDNLQLTNKPFTVTVYDVLGKEHLTQNTHAKTLDISTI
                     TPGLYVLHLQQGDKMYYGRFVKE"
BASE COUNT          385 a          222 c          201 g          325 t
ORIGIN      
        1 ttgaggtgat aagagaaatc gggaaagtag cgcgccatac tccaacggca tgcaccggtg
       61 tataatgcat tgtgaatggt aaaaacggat ttaataccac gcatgcgttc aaacttataa
      121 caatgctcca ccataaaagg tataaggcct gtatgatgat cgtggcagtg gagtatttcg
      181 ggcaactcgg cccaggtatt aatccaatcc aatgctgcat gttgaaaaaa caaaaaacgc
      241 tcggggtcgt cactgtaacc atataagttt tcgcgataga gtaaatacgg caagtcaaca
      301 aaatataagt cgaaaccaaa atcaaccata gatttgcgaa tcatgtaatc aacaccataa
      361 tgctcgcttt tcatatatcc atgatgcacg ggcactgtat tatttgccgc atcccatttg
      421 ctattgtaac gtggcataat tacgcaagtg ctgcaacctg catcgtttaa atattttggc
      481 aaagctccgg caacatcgcc taagccgcct gtttttgcta ccggataaac ctcggccgaa
      541 aaatgtactg ccctgtattg catataactg ctttttaaag tggatgcgct aaaagtaatt
      601 gcatacgcca atggaacaaa gaaaaaatga aattagttgc aggaaagttt taaagcaaaa
      661 tgttgtcgtc catctaacca caaactacca ctttatataa actaagcagg ccggccaaaa
      721 gcccactgaa aaaagtatga cagtacaata ccataagttt ctaatgtatg cttgccgcca
      781 attagttcgt tataaaataa aaattacttt ataaattgat atttaaaagt gttttcgaaa
      841 ctaattagca agtaattcac attataattt atataaaaag ggtagttaaa aaaacaaata
      901 agattgcaat caatttttac ccaccgctca ctccttaaca aacctgccat aatacatttt
      961 atcgccttgc tgtaaatgca gtacgtataa gcccggtgtt attgtgctaa tatctaaagt
     1021 tttggcatgg gtgttttgtg ttaaatgctc tttacccaac acatcatata cagtaaccgt
     1081 aaacggttta tttgtcaatt gtaaattatc aattgttaat tgattacttg ccg
//
LOCUS       DAOMAA010000915         1132 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_171040_length_1132_cov_1.857939, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000915 DAOMAA010000000
VERSION     DAOMAA010000915.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171040_length_1132_cov_1.857939"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1132
                     /locus_tag="PLO59_10730"
     CDS             <1..>1132
                     /locus_tag="PLO59_10730"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HQV01627.1"
                     /translation="LRIFFHALGLDFSAAAYLTSFPVLILIVHQYWHASFLNKLTQIY
                     FIAVIVLSVLISVANTVLYKFWLQLLGYRAIQFLADPNEILASVNGMQAIMFIAFFVI
                     SVSAIVALYFKINEPLQVLPNILLRMALLPALGFITFYFIRGGLQVIPINESAAYFSN
                     NTVSNHAAVNPQWYFIRNMQLAGRAGSNPLAFNPASENEKQFNAMMHIAADTQQLISG
                     NANLLFIILESHTADVVAALGGDSGNSPCMDAIIKNDALTFTQCYASGFRTDQMLPSL
                     FSGFPAQNNNSIIRHSDKVHALPHLPKMFAAHNYHNAFYYAGEMNFANMKSYLVQSGF
                     KALIDKNNFKPQQLNSKWGAHDGFAFDKLLTDLDKQQQPFFTS"
BASE COUNT          322 a          215 c          216 g          379 t
ORIGIN      
        1 ctacgtattt tttttcatgc tttagggtta gatttttcgg cagcggcata tcttacttca
       61 tttccggttt taattttaat cgtgcatcaa tattggcatg catccttttt aaataagctt
      121 actcaaattt attttatcgc tgttattgtt ttatccgtgc ttattagtgt tgccaatacc
      181 gttttatata agttctggtt gcaattgttg ggctacaggg ctattcagtt tttagccgac
      241 cctaacgaaa tattggcatc ggtaaatggc atgcaagcca ttatgtttat tgcatttttt
      301 gtaatttccg ttagtgcaat tgttgcattg tattttaaaa ttaatgagcc attgcaagtt
      361 ttgcccaaca ttttgttgcg aatggcatta ctacccgcat tgggttttat tacgttttac
      421 tttattcgtg gtggtttgca ggttataccc ataaatgaaa gtgcagctta tttcagtaac
      481 aacaccgtaa gtaatcatgc agcggttaac ccgcaatggt actttatacg aaacatgcaa
      541 ttggcaggca gggcaggtag taatccgctg gcatttaatc cggcttctga aaacgaaaag
      601 cagtttaatg ccatgatgca tattgcagcc gatacacaac agctaatttc cggcaatgct
      661 aatctgttgt ttattattct cgaaagtcat acagcagatg tagtagcagc attaggaggc
      721 gatagcggca attcgccttg catggatgcc attattaaaa acgatgcatt aacgtttaca
      781 caatgttatg ccagcggctt tcgaacggac cagatgttgc cttcgttgtt tagtggtttt
      841 cctgctcaaa ataacaacag tattatacgc cacagcgata aagtgcatgc attacctcat
      901 ttacccaaaa tgtttgctgc acataattac cacaacgcat tttattatgc gggcgaaatg
      961 aactttgcca atatgaaaag ctatttggta caaagcggat ttaaagcatt aattgataaa
     1021 aataacttca aaccccaaca gctaaacagc aaatgggggg cgcatgatgg ctttgcattt
     1081 gataaattat taaccgattt agacaaacaa cagcaaccct tttttacgtc tt
//
LOCUS       DAOMAA010000916         1131 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_171306_length_1131_cov_2.079926, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000916 DAOMAA010000000
VERSION     DAOMAA010000916.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171306_length_1131_cov_2.079926"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(825..>1131)
                     /locus_tag="PLO59_10735"
     CDS             complement(825..>1131)
                     /locus_tag="PLO59_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017258321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HQV01628.1"
                     /translation="TALIDTGSRMDEVIFEEFKGTGNMELQLDRKIANKRIWPAIDLV
                     ASSTRREDLLLSKDVLQKMWILRNHLADMNPLEAMEFIQERMRGTKSNEEFLISMNS"
BASE COUNT          389 a          191 c          198 g          353 t
ORIGIN      
        1 gtgcattgct tgtttgccag ttatgccaat aacctaataa ggtttgcgca tggctataat
       61 ggagcaacat ggtgtaggct agtaaaaaaa agagtgtgaa gagatttaat ttttttgtca
      121 tatataagag ctttgatgga aagggggttt aaataagaaa aataaaaagg attaaatcga
      181 aatgaagcgg taaatataaa aagtacacct gagtacgata tttaagaggc ctgtaattta
      241 attgcaaaaa tggcaattaa aaacattagg tggatagcgt atcgaagcca aaattaccaa
      301 aacgaacacc gttaatataa ttggcaataa gctaattata tgcaattact tcattttgaa
      361 tctttgatca aattgcgttt tcagcatcat tccgcgcata taaaacacaa aataaattat
      421 ggccaaaggc tgaaacaaca cacagctttg caattgtaat tatgttgttg aaagcaaacg
      481 cattcgaaca aaaaaagcaa ataattgtaa cagcaattat gtttgacagc cggtgtagta
      541 acctttacat aatgcttagt tttaaattta agtaacgctt tagccaccct atatagagca
      601 gtatgtttta caacccccaa ttcattattt tataagaatt aaactgtaat caacaataaa
      661 ttgatggttg gattgtatgc ttttggcaat gtgcggcata taaccattat catatggaaa
      721 agtattattt gttgccagcc aaaatattac tgaaaattta aggcatttca tcaacgcctg
      781 taaatttaag tatgtatcaa gttgtatgta tagtaaccgt tacactacga gttcatcgaa
      841 attaaaaact cttcattact ttttgtgccg cgcatacgtt cctgtataaa ctccatggct
      901 tcaagcgggt tcatatcggc taagtggtta cgcagtatcc acattttctg taatacatct
      961 ttgcttaata ataaatcttc tctgcgtgta cttgaggcaa ctaaatcaat tgcaggccaa
     1021 atgcgcttat tggcaatttt acggtcgagc tgcaactcca tgttgcctgt gcctttaaac
     1081 tcttcaaaaa taacctcatc catacggctg cctgtatcta ttaaggccgt a
//
LOCUS       DAOMAA010000917         1130 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_171579_length_1130_cov_2.299535, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000917 DAOMAA010000000
VERSION     DAOMAA010000917.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171579_length_1130_cov_2.299535"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            178..>1130
                     /locus_tag="PLO59_10740"
     CDS             178..>1130
                     /locus_tag="PLO59_10740"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HQV01629.1"
                     /translation="MPVTTIAQTWILFNQSNSGLPDNAVKCLAFDNSNVLWIGTDNGL
                     ARFDGTNWNTYNTGNSGIASNQIRSIAFDSLNRLWVGTFNNGACSFDGTNWTVFNTQN
                     SSIASNTIKGISTAPNGDVWFATTNGLSVYNDTLFTTYNIFNSGLATNHFTHVAVGLN
                     QLFIGSINGGFATYDSLFHAYTIVNSGFPDNTTLSTALDTNQKAWIATPSSGLVVYLN
                     PNSWITYNTNNSGMPVNSLNDLCFDGVGNLWMTSPTQGLILKNATNFTHYTTQNSALP
                     SNQLHCLFYNQGILWIGSTFDGLVKLSFNAGINNHYNNDLL"
BASE COUNT          352 a          189 c          197 g          392 t
ORIGIN      
        1 aaagggaaac aaatgtaaaa aactttagaa acgctgaatt attaattcta tttacttgtt
       61 tattaagtga atactgcaaa ctaccgcttt caataatttt tgttttagtt tcgctctgta
      121 taacatttaa gcatttacat ggtgagagat attattttct gtttgctaat atttttaatg
      181 cctgtaacta caattgctca aacatggata ctgttcaatc aatccaactc aggcttgcct
      241 gataatgccg ttaaatgttt ggcttttgat aacagtaatg tactttggat tggtaccgat
      301 aatggcttag cacgttttga tgggactaat tggaatactt ataataccgg taatagtgga
      361 attgcatcaa atcaaatcag aagtattgct tttgactcac taaaccggct atgggtgggt
      421 acttttaata atggcgcatg tagttttgat gggactaatt ggaccgtttt taatacacag
      481 aatagtagca tagccagcaa cacaataaaa ggcataagta ctgcaccaaa cggtgatgtt
      541 tggtttgcca ccaccaatgg gttaagtgtt tataatgata ctttatttac tacgtacaat
      601 atttttaata gtggcttagc aaccaatcat tttacgcatg tggccgttgg tctaaatcaa
      661 ttatttattg gtagcataaa tggtggcttt gcaacctatg attcattgtt tcatgcatac
      721 accatagtaa actcaggttt ccctgataac acaacactta gtacagctct tgatacaaac
      781 caaaaagcat ggattgcaac accgtcatcg ggtctggttg tttaccttaa tcccaatagt
      841 tggattactt ataataccaa caactctggt atgccggtta actctttaaa tgatttgtgt
      901 tttgatggtg ttggaaatct atggatgacc tcacctacac aaggtttgat tttaaaaaat
      961 gcaaccaatt ttacacacta tactacacaa aacagtgcat taccatctaa tcaattacat
     1021 tgtttatttt ataatcaggg tatattgtgg attggcagta cctttgacgg cttagttaaa
     1081 ttgagtttta atgccggcat taataaccat tataataatg atttgctttt
//
LOCUS       DAOMAA010000918         1130 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_171607_length_1130_cov_2.119070, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000918 DAOMAA010000000
VERSION     DAOMAA010000918.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171607_length_1130_cov_2.119070"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="PLO59_10745"
     CDS             complement(<1..644)
                     /locus_tag="PLO59_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01630.1"
                     /translation="MFLAIYNYEIAYWLKRPATCIYAFVAIVAGIIVMAANAIGFDYS
                     TFQTFRIANAPYALYQQSVLFFKLLLFIIPAVIGGAFYRDFESKVFHILYSFPINKSV
                     YLCSKFLSAFTIVLSVTALFMLGCWLGTQLPNAKPYLLHSFQLSSYTNLFFIYLIPNV
                     LFFSLVSFAVVLFTRNIYTCFIVTVVIIILREILLRATTGYNAITVYGLFDPLC"
     gene            complement(651..>1130)
                     /locus_tag="PLO59_10750"
     CDS             complement(651..>1130)
                     /locus_tag="PLO59_10750"
                     /inference="COORDINATES: protein motif:HMM:NF039197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5694 domain-containing protein"
                     /protein_id="HQV01631.1"
                     /translation="HPGQFGYYYSQLENYASQHNQQSLLKGIGKGITIPQSDLIDEDS
                     LRNNISLLAYMRWLNSKQVQTSAHAHYINLYPQMGNTNVFVYDSTYFLGAELTADWYR
                     RNIKIYSKIIAQLNYSEHAIFLIMGNDHIPIIRHLFEGNPFFKVMDTEMWLGKSELK"
BASE COUNT          409 a          199 c          195 g          327 t
ORIGIN      
        1 tcgcacaagg gatcaaaaag cccgtaaaca gttattgcat tataaccggt tgttgcccgc
       61 aataatattt cgcgaagtat aattataacc accgtaacta taaagcatgt ataaatattt
      121 ctagtaaaaa gtaccacagc aaatgatacc aaactaaaaa acaaaacatt tggaatcagg
      181 tatataaaaa acaagtttgt ataactgctc agttggaagc tatgcagtaa atatggcttt
      241 gcatttggca attgcgtacc aagccagcaa cccagcataa acaatgcagt aacagataaa
      301 acgattgtaa aagcgcttaa aaatttcgaa cacaggtaaa ctgatttatt aataggaaag
      361 ctatagagta tgtgaaacac tttactttca aaatcgcgat agaatgcacc accaattaca
      421 gccggtatta taaataagag caacttaaaa aataacacag attgctgata aagcgcataa
      481 ggtgcatttg cgatgcgaaa agtttgaaaa gttgaataat caaaaccaat ggcattagca
      541 gccataacta ttatgcctgc aactattgca acaaatgcat atatgcaggt tgcaggtcgt
      601 ttaagccaat aagctatttc gtaattatag atagcaagga acatgatgcc ttattttagt
      661 tcgcttttac ctaaccacat ttctgtatcc ataactttaa aaaatggatt gccttcgaac
      721 agatgacgga ttataggaat atgatcgtta cccatgatca gaaaaatagc gtgctcactg
      781 taattaagtt gcgcaataat ttttgaatag attttaatat tacgtctata ccagtcggct
      841 gttaattcag ctcccaaaaa ataagtcgaa tcatatacga acacatttgt attacccatt
      901 tgcgggtata ggtttatgta atgtgcatga gcagatgttt gaacttgctt tgaattaagc
      961 caacgcatat atgccagtaa acttatgtta ttacgtagcg agtcttcatc aattaaatcc
     1021 gactgaggta tagtaatacc ctttccaatg ccctttaata atgactgttg attatgttgc
     1081 gaagcataat tttctaattg cgaataatag tatccaaact gcccgggatg
//
LOCUS       DAOMAA010000919         1128 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_172265_length_1128_cov_1.867661, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000919 DAOMAA010000000
VERSION     DAOMAA010000919.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172265_length_1128_cov_1.867661"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1128
                     /gene="dnaK"
                     /locus_tag="PLO59_10755"
     CDS             <1..>1128
                     /gene="dnaK"
                     /locus_tag="PLO59_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HQV01632.1"
                     /translation="NDAQRQATKEAGEIAGLKVLRIINEPTAAALAYGLDKKHSDTKV
                     AVFDLGGGTFDISVLELGDGVFEVKSTNGDTHLGGDDFDHVIIDWLANEFMSDEGLDL
                     RKDPMAMQRLKEAAEKAKIELSSSMQTEINLPYIMPVDGIPKHLVKTLTRAKFEQLAD
                     SLIQRTLEPCKKALSDAKLTATQIDEVILVGGSTRIPAIQELVEKFFGKKPSKGVNPD
                     EVVAVGAAIQGAVLTGEVKDVLLLDVTPLSLGIETLGGVMTKLIESNTTIPTRKTETF
                     STASDNQPSVQILVCQGERPMAKDNRKIGEFNLDGIMPAPRGVPKIEVTFDIDANGIL
                     HVTAKDGATGKEQKIRIEASSGLSDAEIKRMKEEAERNADA"
BASE COUNT          374 a          216 c          256 g          282 t
ORIGIN      
        1 ttaacgatgc ccagcgccag gctaccaaag aagcaggcga aattgcaggc cttaaagtgt
       61 tgcgtattat aaacgaacct acagctgcag cgttggctta tggcttagat aaaaaacaca
      121 gcgataccaa agtagccgta ttcgatttag gtggcggtac cttcgatatt tctgtacttg
      181 aattaggtga cggtgtattt gaagtaaaat caaccaatgg cgatacccat ttaggtggcg
      241 atgattttga ccatgtgatt attgactggt tagccaatga gtttatgagc gatgaaggct
      301 tggatttacg caaagaccca atggcaatgc aacgccttaa agaagctgcc gaaaaagcaa
      361 aaatcgaatt gtcgagcagt atgcaaaccg aaattaacct gccttacatc atgcctgttg
      421 atggcattcc aaaacacttg gtaaaaacat taacacgcgc caagtttgaa cagttagctg
      481 atagcttaat acaacgcact ttagagcctt gtaaaaaagc attaagcgat gccaaactta
      541 ctgcaacgca aattgatgaa gtaattttag tgggcggttc aacccgcata cccgctatac
      601 aagaattggt agaaaagttt tttggtaaaa aaccaagtaa aggcgttaac cctgacgaag
      661 tggtagccgt aggtgcagcc atacaaggcg ctgtattaac cggtgaagtt aaagatgtat
      721 tgttgttaga tgttacacca ttatcgttgg gtatcgaaac cttaggtggt gtaatgacta
      781 aattaatcga atcaaatacc accatcccaa cacgcaaaac cgaaaccttt agtacagcca
      841 gcgacaatca gccatcggta caaattttag tgtgtcaggg cgaacgtcct atggctaaag
      901 acaaccgtaa aattggtgag tttaacttag atggtataat gcctgcgcca cgtggtgtgc
      961 caaaaattga agttactttc gatattgatg ccaatggtat tttacatgtt actgcaaaag
     1021 atggtgcaac aggtaaagag caaaaaatac gtatcgaagc atcgagcggt ttaagtgatg
     1081 cagaaataaa acgcatgaaa gaagaggctg aacgtaatgc cgatgccg
//
LOCUS       DAOMAA010000920         1124 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_173292_length_1124_cov_2.787652, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000920 DAOMAA010000000
VERSION     DAOMAA010000920.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_173292_length_1124_cov_2.787652"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="PLO59_10760"
     CDS             <1..498
                     /locus_tag="PLO59_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010665169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQV01633.1"
                     /translation="SALFDSLTVEPNVAFPLKMFTDMTAAQRLHRANECIKRVKLENA
                     NKLYPSEISGGMKKRVAIARAIANNPKYLFCDEPNSGLDPVTSIVIDKLIVEITQEYN
                     MTTIINTHDMNSVIEMGERIVFLHKGKLQWQGTNTELLSVKDKTVIDFVFATQIMQAL
                     RNDSK"
     gene            complement(500..991)
                     /gene="cdd"
                     /locus_tag="PLO59_10765"
     CDS             complement(500..991)
                     /gene="cdd"
                     /locus_tag="PLO59_10765"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020528010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HQV01634.1"
                     /translation="MDVIKTEIIYKSYHGIDSLPALEKNLMLAAMQAAQSAYAPYSSF
                     FVGAAAQLENGTIVLGSNQENAAYPSSLCAERVALFSVGAQHPDIPVVAIAVTAFMGN
                     LDNQHPVSPCGACRQVMSEFEHRYGKPVKMIMMASPNQIIVLESVKQLLPFTFNSDSM
                     KST"
BASE COUNT          366 a          227 c          206 g          325 t
ORIGIN      
        1 agtgcattat tcgactcgct tacggtcgaa ccaaatgtgg ctttcccatt aaagatgttt
       61 accgatatga cagcagcgca acgcctgcat cgtgccaacg aatgtattaa aagagttaag
      121 ctcgaaaatg caaacaagct ttatccaagc gaaattagtg gtggcatgaa aaaacgcgta
      181 gccattgcac gtgcaatagc aaacaatccc aaatatttgt tttgtgatga gcccaactca
      241 gggctcgacc cggtaacctc tatcgtaatt gataagctta tagttgaaat tacgcaagag
      301 tataatatga ctaccataat caatacacat gatatgaact ctgttatcga aatgggcgaa
      361 cgtattgtgt ttttacataa aggtaaatta caatggcaag gcaccaacac tgaattgctc
      421 agcgtgaaag ataaaactgt tattgacttt gtttttgcta cacaaataat gcaagcctta
      481 agaaacgaca gcaagtaagt tatgtacttt tcatactgtc gctattaaaa gtaaagggca
      541 gtaattgttt tacactctcc aatacgataa tttgatttgg gcttgccatc attatcattt
      601 ttacgggctt gccatacctg tgttcaaatt cgctcattac ttgccggcag gcaccacatg
      661 ggcttacggg atgctggtta tctaaattac ccataaatgc cgtaacagca atagctacaa
      721 cagggatatc aggatgctgc gcaccaacag aaaacagagc tacacgctca gcacataagc
      781 tgcttggata ggcagcgttt tcctggttac tacccaaaac aattgttccg ttttctaatt
      841 gcgctgccgc acccacaaaa aaactactgt atggtgcata agccgattgg gcagcttgca
      901 tggcagccaa catcaagttt ttttctaatg caggtaaact gtcaatgccg tgatagcttt
      961 tataaattat ttcagtttta atcacatcca tgtaaaaatg tattatgagg aaacgataat
     1021 gtattaattt attaacattg cgctaatgta cgatagcctt ttttttcaat tcaaaaagtt
     1081 tttcgtacaa ataatttccc caaaaaactc aaaataataa tgaa
//
LOCUS       DAOMAA010000921         1123 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_173578_length_1123_cov_3.055243, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000921 DAOMAA010000000
VERSION     DAOMAA010000921.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_173578_length_1123_cov_3.055243"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..>1123)
                     /locus_tag="PLO59_10770"
     CDS             complement(133..>1123)
                     /locus_tag="PLO59_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009193473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HQV01635.1"
                     /translation="QNNIRNFYSVSISGYHIAEAGANPISQLAFTLANGFTFVEYYVS
                     RGMDVNDFAPNLSFFFSNGIDPEYSVIGRVARRIWAKAMKYKYKANERSQMLKYHIQT
                     SGRSLHAQEIDFNDIRTTLQALYAIYDNCNSLHTNAYDEAITTPTEGSVRRAMAIQLI
                     INRELGLAKNENPLQGSFIIEELTDLVEEAVLKEFDAITERGGVLGAMETMYQRGKIQ
                     EESLYYETLKHTGAYPIIGVNTFLSSQGSPTIVPAEVIRATEEEKESQIQTLQNLHSR
                     NKNTREKLLHDLQLAAVQNKNTFEQIMEVAKHCSLGQITAALFEVGGQYRRNM"
BASE COUNT          302 a          218 c          222 g          381 t
ORIGIN      
        1 ggtttcagcg gtttataaac ttgatcttac gatttacaca ttttaataaa ttcaggtatg
       61 cgtagcgcat ggtatcagtt aatttgcatc ctgattttat gtaacgtcaa aaaataattc
      121 cttttgatag ttttacatat tacgtctgta ttggccacca acttcaaata gagccgcagt
      181 aatttgacct aatgagcaat gtttggctac ttccataatt tgttcgaagg tgtttttatt
      241 ttgtactgct gccaactgca aatcatgcaa taatttttcg cgagtgttct tattacggct
      301 atgcaaattt tgtaaggttt gaatttgact ttctttttcc tcttctgtgg ccctgattac
      361 ttctgccggt actatagtag gcgagccttg tgatgataaa aatgtattta caccaattat
      421 aggataggcg cctgtatgtt tcagggtttc atagtataaa ctctcttctt gaattttgcc
      481 acgctgatac atggtttcca tggcacccaa aacgccacca cgttcggtaa tggcatcaaa
      541 ctcttttaat actgcttctt caaccaggtc ggtaagttct tctataataa aagagccttg
      601 caacgggttt tcattttttg ccaggcccaa ttcgcggttt attattaact gaatggccat
      661 agctctgcgt acactgcctt cggtaggtgt ggttatggct tcgtcataag cattggtgtg
      721 taatgagttg cagttatcat aaatggcata caatgcttgt aatgttgtac ggatatcatt
      781 aaagtcaatt tcctgtgcat gtagcgaccg tcccgaagtt tggatatggt acttaagcat
      841 ttgcgatcgt tcgttggctt tatatttata cttcatagcc ttagcccaaa ttctacgtgc
      901 tacccgacca attaccgaat attcggggtc aataccatta ctaaagaaga atgataaatt
      961 gggtgcaaaa tcattaacat ccatgccgcg gcttacatag tattcaacaa aagtgaaacc
     1021 attagccaac gtaaatgcca attgcgaaat gggattggcc ccggcttcgg caatgtggta
     1081 accgctgatg gaaaccgagt aaaaattgcg tatgttattt tgt
//
LOCUS       DAOMAA010000922         1123 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_173597_length_1123_cov_2.750000, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000922 DAOMAA010000000
VERSION     DAOMAA010000922.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_173597_length_1123_cov_2.750000"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1123)
                     /locus_tag="PLO59_10775"
     CDS             complement(<1..>1123)
                     /locus_tag="PLO59_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HQV01636.1"
                     /translation="AYTGYVKVGNTTGIANTTYVDNNNGAGLINGIDYCYMIVATFAD
                     GAESYASVEVCTQLKRDLPVITNVSINTTNATAGQVYVAWSKPTELDTTQTPGPYKYV
                     ISGSSDFTGGAFALIDSFFDLNDTILIDDFVNTQTLPRSYKIEFYNMTPGNTFKIGQS
                     QIASSIFLNCVPTDEATTLSWNETVPWSNTLYTIYRQNTSGIFDSIGTSATQSYTDTG
                     LVNGNTYCYKIRSTGNYSATGFVDPIINFSQEQCAIPVDNVAPCTPALMVNANCIGFN
                     NVLSWHFDSAACYQDIAGYNVYYKNDSINDLVLLVTLGLDTNFLHDSLFSIAGCYQVT
                     AFDSLGNESALSNKVCVDNCPVYELPNIFSPNDDGKNDTF"
BASE COUNT          343 a          229 c          209 g          342 t
ORIGIN      
        1 gaaacgtatc atttttacca tcatcgttgg gcgaaaaaat atttggcaat tcatataccg
       61 ggcaattatc cacacacacc ttattactta aagcactttc atttcccagg ctatcaaagg
      121 cagttacctg gtaacaacct gcaattgaaa aaagactatc atgtaaaaag ttggtatcta
      181 aacctaaggt aacaagcaac accaaatcgt ttatcgaatc atttttataa tacacgttgt
      241 agcctgcaat atcctgataa catgcagcac tatcaaaatg ccagcttaaa acattattaa
      301 atccaataca attggcatta accataagcg caggtgtaca tggcgccacg ttatcaacag
      361 gtatggcaca ttgctcttgc gaaaaattta ttatcggatc aacaaagccc gttgcactgt
      421 aattgccggt acttctgatt ttgtaacaat aggtgttgcc gtttaccagt ccggtatccg
      481 tataactttg agtagcactt gtacctattg aatcaaaaat accggaagtg ttttgacgat
      541 agatggtata aagcgtattg ctccagggca ccgtttcatt ccacgaaagt gttgtggctt
      601 cgtcagtggg cacacagtta agaaatatgg atgaagcaat ttgcgattgc cctattttaa
      661 atgtattgcc cggagtcata ttataaaatt caattttata actgcgtggt aaggtttgtg
      721 tgtttacaaa atcatcaata agtatggtgt cgtttaaatc aaaaaacgaa tcaattaatg
      781 caaaggctcc tccggtaaaa tcgctgctgc cactaattac gtatttgtac ggcccgggtg
      841 tttgtgttgt gtctaactca gtaggtttac tccatgctac atatacctgg ccggccgtag
      901 catttgttgt attaatacta acattggtaa ttacaggcaa atcgcgcttt agctgcgtgc
      961 atacttcaac agaggcataa ctctcggctc catcggcaaa ggtggccact atcatataac
     1021 agtaatcaat accatttata aggccggcac cattattatt atctacgtat gttgtattgg
     1081 caatgcctgt tgtgtttcca acttttacat aacctgtata agc
//
LOCUS       DAOMAA010000923         1121 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_174172_length_1121_cov_3.541276, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000923 DAOMAA010000000
VERSION     DAOMAA010000923.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_174172_length_1121_cov_3.541276"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1121)
                     /locus_tag="PLO59_10780"
     CDS             complement(<1..>1121)
                     /locus_tag="PLO59_10780"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HQV01637.1"
                     /translation="IAFKLLSNNYNPFLPTDNILYYVLEEVLQGAMVTAYIIFAAQTL
                     EVAFHKNYIRTLIFIFFTFSVGFLGHHIYDAILNGPGVKSLEVYVISRFTLVGIATLA
                     LLLVWQIRRSTFHRTIIIGSFVYNFSWLVSIVSLSTQTNMLGLNGTELYLLGCLLDII
                     IFSSALGYRVKTMADEKNELVRKEAEARLAIEKTRTGIAMNLHDDIGSVLSSMSVYGE
                     AAKRALKENNVGKADELIEKIGINARETMDNMSDIVWTINPVNDNGIKLFNKIEAFAS
                     SVLNAKEIELKFDVDNELYEKDFLMPVRQNVFFIFKEIITNCVKYSGATTVGVTFTLN
                     SKNLLLLVCDNGKGFDTKNNQHKNASGGNGLKNIKERASV"
BASE COUNT          346 a          224 c          180 g          371 t
ORIGIN      
        1 actgatgcac gctcttttat gttttttaaa ccattgccgc ctgatgcgtt tttatgttga
       61 ttgttttttg tgtcaaaacc ttttccatta tcgcaaacca gcagcagcag gtttttacta
      121 tttaatgtaa aggtaactcc gacagttgta gcacccgaat actttacaca gtttgtaata
      181 atttctttaa aaataaaaaa cacattttgt cttaccggca ttaaaaaatc tttttcatac
      241 aattcattat ccacgtcaaa cttcagttca atttcttttg catttaaaac agaggaggca
      301 aaagcctcta ttttattaaa tagttttatc ccattatcat taactggatt aatggtccaa
      361 acaatgtcac tcatgttgtc cattgtttcg cgtgcattaa ttccaatctt ttcaatcagt
      421 tcatcggcct tgccaacatt gttttcttta agtgcgcgtt tggctgcttc gccatacaca
      481 ctcatactac ttaatacaga gccaatgtcg tcatgcaagt tcatggcaat tcctgtccgg
      541 gttttttcta tggctaaacg ggcttccgct tcctttctta ccaattcatt tttttcatct
      601 gccatggttt ttacccgata tcctaaggca ctcgaaaaaa taataatatc taacaggcat
      661 cccaacagat agagttctgt accatttaaa cctaacatat ttgtttgggt tgaaagtgaa
      721 acaatgctta ctaaccacga gaaattataa acaaaagatc cgataatgat ggtgcggtga
      781 aatgtgcttc tgcgtatctg ccacacaagc agcaatgcta aggtggcaat acctacaagt
      841 gtaaatcttg aaataacata aacctctaat gattttacac cagggccatt taaaattgca
      901 tcgtaaatat ggtggcccaa aaacccgact gaaaaagtaa aaaaaatgaa tataagtgtc
      961 cggatataat tcttgtgaaa tgctacctct aatgtttggg cagcgaatat gatataggcc
     1021 gtaaccattg caccttgcaa tacttcttct aatacgtaat acaaaatatt atcggttggc
     1081 agaaaaggat tgtagttgtt acttagcagt ttgaatgcaa t
//
LOCUS       DAOMAA010000924         1121 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_174244_length_1121_cov_2.648218, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000924 DAOMAA010000000
VERSION     DAOMAA010000924.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_174244_length_1121_cov_2.648218"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /locus_tag="PLO59_10785"
     CDS             <1..829
                     /locus_tag="PLO59_10785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQV01638.1"
                     /translation="DNTVRIVEAYTGAHKNITLLHNVNRTVPYALNMAIPICKYDVIV
                     RLDAHTCYAMDYFEKIIETFDKTNAHIVGGPMHAIGKTTLQKAIAQATSSVMGVGNST
                     FHDINASGYVDSVYLGAWQKSIFEKTGLFDVRFKRNQDDEFHYRAKQMGLKIYLNPEI
                     KSYYYPRNSYTKLSKQYFQYGLYKPMVLQKVKSAIKLRHLIPAAFVLYIMAILPFSLV
                     WFFLIIPLLLYVLLASFYSVKSNGSCKQKLFQFIAYPVIHFSYGCGFIFGFIKHPVA"
     gene            829..>1121
                     /locus_tag="PLO59_10790"
     CDS             829..>1121
                     /locus_tag="PLO59_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01639.1"
                     /translation="MCWPLLLNTALIMLAYFLPIKPQISTIAIVVSFILSLLFIKGDS
                     YKSILHNKPALLLLLFYLMHIVALLYSDNLKYGFKDLEYKLSFIVFPLIFINAG"
BASE COUNT          362 a          174 c          184 g          401 t
ORIGIN      
        1 agataataca gttcgtattg ttgaagcata taccggtgcg cataaaaata ttacattact
       61 ccataatgta aaccgcacgg taccttacgc tttaaacatg gccattccaa tttgtaaata
      121 cgatgtaatt gtaaggttag atgcacatac atgttatgca atggattatt tcgaaaaaat
      181 tatcgaaaca tttgataaaa ccaatgcaca tatagttggc ggacctatgc atgcaattgg
      241 taaaacaacg ttgcaaaaag caattgctca ggcaacctca tccgtaatgg gtgtaggcaa
      301 cagtacgttt catgatataa atgcaagtgg ttatgtggat agcgtttatt taggcgcatg
      361 gcaaaaaagt atttttgaaa agaccggctt atttgatgtt cgctttaaac gaaatcagga
      421 tgatgaattt cattacaggg ccaaacaaat ggggcttaaa atttatctga atcccgaaat
      481 taaatcatat tactatccac gtaactctta tacaaaatta agtaaacaat actttcaata
      541 tgggctttac aaacctatgg ttttgcaaaa agtaaaatca gcaatcaaac tcaggcattt
      601 aataccggct gcttttgtac tttacataat ggccatttta ccatttagtt tggtatggtt
      661 ttttttaatt atacccttgt tattatatgt gttattggct tcgttttatt cagttaaaag
      721 taatggctct tgtaaacaaa aactatttca atttatagca tatccggtaa ttcatttttc
      781 atatggatgc gggtttattt ttggttttat taaacatcct gttgcataat gtgctggccg
      841 ctacttttaa atacagcctt aattatgttg gcatattttc tgccaattaa gccacaaata
      901 agtacaattg ccatagttgt aagctttata ctttcattac tttttattaa aggcgatagc
      961 tataaaagta tattgcataa taagccggct ttacttttac tgttatttta tttaatgcat
     1021 atagttgcgc ttttatactc tgataattta aaatacggtt ttaaagattt agagtataaa
     1081 ctgtcgttta ttgtttttcc attgatattt ataaatgccg g
//
LOCUS       DAOMAA010000925         1121 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_174262_length_1121_cov_2.497186, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000925 DAOMAA010000000
VERSION     DAOMAA010000925.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_174262_length_1121_cov_2.497186"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1121)
                     /locus_tag="PLO59_10795"
     CDS             complement(<1..>1121)
                     /locus_tag="PLO59_10795"
                     /inference="COORDINATES: protein motif:HMM:NF018899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HQV01640.1"
                     /translation="NNVSVKAKPGNLKSQLLPIIKQKPNRKILGGIRFHLGAYQLGYL
                     GKVDSAGNRKGFNRWLMQTVGEPPVILDTFLTTKSVNQLKQYMQNCGYFNVVVTDTII
                     YKKNRHANVIYKIIPHQPYIIDTISYKLSDIKIDAILKSNTPATDLKPGQIYNATGLQ
                     KERERISKILRNNGYYNFTQQYVHFLIDSSNNNQKVKVVVVIDNPEIDASSTSESDLF
                     HQPYYINQTYVLPQFDILDKVDNTQATDTILKNRVYFLIKSNKSMPFKKAIIEQHIFL
                     QPGTLYRQKDVDLTYQRLQDLGVFRFINIAMTQLPLTTPDDSSTLNDTAKLVNAYIQL
                     TKADQQDYSLENEVTTSGGNVGIAGSIAYRHKNFFRGTE"
BASE COUNT          317 a          188 c          204 g          412 t
ORIGIN      
        1 attcagtacc tctaaaaaag tttttatgcc ggtaagcaat acttcctgca ataccaacat
       61 tgccaccact tgtggttact tcattttcga gtgaataatc ttgttggtcg gctttggtta
      121 gctgtatgta tgcatttaca agttttgccg tatcgtttaa tgtactgcta tcgtcaggtg
      181 tggttaatgg caattgagtc attgcaatat ttataaatcg aaacacgccc aaatcttgca
      241 gtcgttgata ggttaaatcc acatcctttt gcctgtataa ggtgccaggt tgtaaaaaaa
      301 tgtgttgttc gattatggcc tttttaaaag gcattgattt attacttttt attaaaaaat
      361 aaactctgtt ttttaaaatg gtatcggtgg cttgtgtatt atcaacctta tctaaaatgt
      421 caaattgcgg caatacatag gtttggttaa tgtaatatgg ctgatgaaac aaatcacttt
      481 ccgaggtgct ggaggcatca atttcaggat tatcaataac caccaccact tttacctttt
      541 gattattatt tgaactatca attaaaaagt gtacgtattg ttgtgtaaag ttgtagtagc
      601 cattgtttcg caatatttta ctaatacgct cacgctcttt ttgcaaaccg gttgcattat
      661 aaatttgccc aggctttaaa tcggtagcag gtgtgttgct ctttaaaatt gcatctattt
      721 taatgtctga taatttgtaa ctaatggtat caattatata gggctggtgt ggtattattt
      781 tataaataac attggcatgc cggttctttt tataaataat tgtatcggtt acaaccacat
      841 taaaataacc acaattttgc atgtattgtt ttaactgatt taccgatttg gttgtaagaa
      901 atgtatctaa aattaccggt ggctcgccta cggtttgcat aagccagcga ttaaagcctt
      961 tgcggttacc tgccgagtcc accttaccca aataacctaa ttggtatgca cctaaatgaa
     1021 aacgaatgcc tcctaatatt ttacgattgg gcttttgttt tataataggc agtaattggc
     1081 tttttaggtt tccgggctta gcttttacgg aaacattatt t
//
LOCUS       DAOMAA010000926         1120 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_174526_length_1120_cov_2.845070, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000926 DAOMAA010000000
VERSION     DAOMAA010000926.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_174526_length_1120_cov_2.845070"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..887
                     /locus_tag="PLO59_10800"
     CDS             84..887
                     /locus_tag="PLO59_10800"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007650222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HQV01641.1"
                     /translation="MQIIEDAFNNRSLLNDSQTQQAIRDTIARLDAGSLRVAQPDANG
                     WKVNNWVKQAVIMYFQICEMKTMHAGMLEFYDKMEIKTGYADKGIRVVPHAVARYGAY
                     IAKGAILMPSYVNIGAYVDSGTMVDTWATVGSCAQIGKHVHLSGGVGIGGVLEPVQAA
                     PVIIEDGCFIGSRCIVVEGVHVGTEAVLGANVVLTMSTKIIDVTGAQPVETKGYVPPR
                     SVVIPGSYTKNFNAGSYQVPCALIIGQRKQSTDTKTSLNDALRDFNVAV"
     gene            894..>1120
                     /locus_tag="PLO59_10805"
     CDS             894..>1120
                     /locus_tag="PLO59_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011585068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HQV01642.1"
                     /translation="MSRFLIVQTAFVGDAILMTALLEKLHVYYPQATLDVLVRKGNES
                     LFDSHPFIGNVLIWDKKQGKYKSLFTLINSI"
BASE COUNT          358 a          189 c          241 g          332 t
ORIGIN      
        1 tagagtatta caatattaaa ataattaaaa caataaaatt aaaaatcata atatctacct
       61 tgcccacctt taaaaagtaa actatgcaaa ttattgaaga tgcctttaac aaccgctcat
      121 tgttaaacga tagccaaaca caacaagcca taagagatac tattgccaga ttagatgccg
      181 gcagtttacg tgtagcacaa cccgatgcaa atggctggaa agtaaataac tgggtaaagc
      241 aggctgttat catgtatttt caaatttgtg aaatgaaaac catgcatgcc ggtatgcttg
      301 agttttatga taaaatggaa ataaaaacag gttatgccga taaaggaata agagtagtac
      361 cacatgccgt tgcgcgctac ggtgcttata tagcgaaagg tgcaatactt atgcccagtt
      421 atgtaaatat tggtgcttat gttgatagcg gtacaatggt tgatacctgg gctaccgttg
      481 gctcttgtgc acaaattggc aagcatgtac atttaagtgg tggtgttggt attggtggtg
      541 ttttagagcc cgtgcaggct gcaccggtaa ttattgaaga tggttgtttt attggttcgc
      601 gatgcattgt tgttgagggt gtgcatgtag gtaccgaagc cgttttaggt gccaatgtgg
      661 ttttaaccat gtcaaccaaa ataattgatg ttacaggtgc ccaacctgtg gaaacaaaag
      721 gatatgtacc accgcgcagt gtggttatac ccggttcgta cactaaaaat tttaatgctg
      781 gcagctatca ggttccatgt gctttaataa tcgggcagcg caaacaaagt accgatacca
      841 aaacatcgct aaatgatgcc ttgcgcgatt ttaatgttgc tgtgtaatac atgatgagtc
      901 gttttttaat tgttcaaaca gcttttgttg gcgatgccat tttaatgacg gcattactcg
      961 aaaaactgca tgtatattat ccacaagcaa cattagatgt attggttcga aaaggcaatg
     1021 aaagcttatt tgatagccat ccatttattg gcaatgtttt aatttgggat aagaaacaag
     1081 gcaaatacaa aagcttgttt acactaataa acagcatcag
//
LOCUS       DAOMAA010000927         1119 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_174927_length_1119_cov_2.473684, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000927 DAOMAA010000000
VERSION     DAOMAA010000927.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_174927_length_1119_cov_2.473684"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="PLO59_10810"
     CDS             <1..263
                     /locus_tag="PLO59_10810"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV01643.1"
                     /translation="EITNASVLAYPNPTDNILKLNTQLTSYSQASIYLYDVTGRLVYQ
                     KQINTQQNTEELLLGQLDNGYYNYNIIADGVSILNGKVGVIK"
     gene            391..>1119
                     /locus_tag="PLO59_10815"
     CDS             391..>1119
                     /locus_tag="PLO59_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01644.1"
                     /translation="MNLKHLKNLILIACLSNGIATAQLIPVTFGSYSKVPEDFFGYNG
                     ANFVRANEPNYNTLWVVDSLPSLHANTIRYTAGLPGNYWDWQTGTFLKELPPNWILPK
                     DYDGINPKKSDLITFRAMVNRAIITPMLSCNILCSNKEFQLAELFYAKGINIPIKYIE
                     MGSELYHTRLNYVEVFPTPTDYALRANEWASYFKSFPFFANIKIGVVGATQRPEESYS
                     RRNTWTTDVAATVDSDVDAIILHYY"
BASE COUNT          395 a          203 c          193 g          328 t
ORIGIN      
        1 ctgaaattac aaatgcaagt gtactagcat atccaaaccc aaccgataat attttaaaac
       61 taaacaccca attaacttct tatagtcaag catcaatata cctgtacgat gttaccggcc
      121 gtctggttta ccaaaagcaa ataaatacac aacaaaatac tgaagaactg ttattaggtc
      181 aattagacaa cggttactat aattacaata ttattgctga tggagtttct attttaaacg
      241 gcaaagttgg cgtaataaag taagcgacaa gtaagtttta ataaatacct aataagtaag
      301 ctacatttaa gcagtagctc ataaaaataa aggctaaatt tggcctttat ttttttttat
      361 acccgttaaa aaaacacacc acattagaga atgaacttaa agcatcttaa aaacttaatt
      421 cttattgctt gcctttcgaa tggtattgcc acagctcaac tcatacctgt aacatttggt
      481 tcatacagca aagtacccga agattttttt ggttacaacg gggcaaactt tgttagggct
      541 aacgaaccca attataatac actttgggtg gttgactcat taccaagttt acatgcaaat
      601 acaatcagat acacagctgg gttacccggc aactattggg attggcaaac aggcacattt
      661 ttaaaagagt taccccccaa ttggattttg cccaaagatt acgatggaat taatccaaag
      721 aaaagtgatt taattacgtt tagggcaatg gttaacagag ccataattac gcctatgtta
      781 agttgcaata ttttatgcag caacaaagag tttcaattgg ctgaattgtt ttatgcaaag
      841 ggtataaata ttccgataaa atacattgaa atgggtagcg aattatacca cacccgttta
      901 aattatgttg aggtttttcc aacgccaact gactatgcat taagagctaa tgaatgggca
      961 agctacttta aatcattccc attttttgcg aatattaaaa ttggtgttgt gggtgctacc
     1021 caacgacccg aagaaagtta cagccgcaga aatacctgga caacagacgt ggcagcaacc
     1081 gtagatagcg atgtggacgc aataatattg cattattat
//
LOCUS       DAOMAA010000928         1117 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_175441_length_1117_cov_3.583804, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000928 DAOMAA010000000
VERSION     DAOMAA010000928.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_175441_length_1117_cov_3.583804"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..117
                     /locus_tag="PLO59_10820"
     CDS             <1..117
                     /locus_tag="PLO59_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HQV01645.1"
                     /translation="FAAGDVQDHIYRQAVTAAGSGCMAAIDAERWLATQGIH"
     gene            complement(124..501)
                     /locus_tag="PLO59_10825"
     CDS             complement(124..501)
                     /locus_tag="PLO59_10825"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008270266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HQV01646.1"
                     /translation="MKKKSQPKKIPDVKPVNIGVDFYHQVHDVARLVPYGRVTSYGAI
                     AKALAAPKAARLVGYAMHLAHTASKPVPAHRVVNSQGLLTGKHHFGSPFLMQQLLEAE
                     GVEVVNNKVSNFKNLFWNPLTEL"
     gene            648..>1117
                     /gene="rsmG"
                     /locus_tag="PLO59_10830"
     CDS             648..>1117
                     /gene="rsmG"
                     /locus_tag="PLO59_10830"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HQV01647.1"
                     /translation="MHLKLNTHQTNQLKALPQLYAEWNEKINVISRKDITQIETHHIL
                     HSLAIARFINFTPGSSIMDIGTGGGFPGIPLAIVFPQVSFTLVDSIGKKIKVVQAIAK
                     EIGLQNVTAIQQRAEDVASKFDFIVSRATAPMTDLFRWSQNKFNGKQNNPLPNG"
BASE COUNT          334 a          231 c          225 g          327 t
ORIGIN      
        1 tttgcagcag gcgatgtgca ggaccatatt taccgtcaag ctgttacggc tgcggggtcg
       61 ggttgtatgg cagccattga tgccgagcgt tggctggcca cacaaggcat acattaatgc
      121 ggtttacaat tcggtaagtg gattccaaaa caagttttta aagttgctca ccttattgtt
      181 tactacttca acgccctcgg cttccaataa ctgctgcatt aaaaatggcg atccaaaatg
      241 atgtttgccg gttagtaagc cctggctgtt taccacccgg tgggcaggta cgggtttact
      301 tgccgtatgt gctaagtgca tggcataacc taccaaacgg gcggcttttg gcgctgccag
      361 cgcttttgca attgcaccat aagatgtaac gcgtccataa ggtactaaac gcgccacatc
      421 atgtacctga tggtaaaaat caacacctat gtttaccggt tttacatcag gtattttttt
      481 aggttgtgat ttctttttca taatcgttta gccgataatt ggtgtttcat tacaaaaact
      541 aaatttaaaa tattacgctt gccaaaatga aacatgcctt tataacttgc aaataattta
      601 tgcttttgaa tgaagcatta aagcaacaat taaatatttc aatcaaaatg catctcaaat
      661 taaacacaca tcaaaccaat cagctaaaag cattgccaca actttatgcc gaatggaatg
      721 agaaaattaa tgtgatatcg cgtaaagaca taactcaaat tgaaacgcat catatactac
      781 attcattagc tatcgcccgt tttataaact ttacacccgg tagcagtatt atggatattg
      841 gcaccggtgg tggtttcccg ggtattccgt tagccatagt ctttccgcag gtttcgttta
      901 cgttggttga cagtattggt aaaaaaatta aagttgttca agccattgcc aaggaaatag
      961 gcttgcaaaa tgttacggct atacaacagc gtgccgaaga tgtggcctct aaatttgact
     1021 ttatagtgag ccgagctaca gcacccatga ccgatttgtt tcgatggagc caaaataaat
     1081 ttaacggtaa acaaaataat ccgctaccca acggaat
//
LOCUS       DAOMAA010000929         1115 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_176065_length_1115_cov_2.850000, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000929 DAOMAA010000000
VERSION     DAOMAA010000929.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_176065_length_1115_cov_2.850000"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..414)
                     /gene="trxA"
                     /locus_tag="PLO59_10835"
     CDS             complement(94..414)
                     /gene="trxA"
                     /locus_tag="PLO59_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010602362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HQV01648.1"
                     /translation="MALELTDANFDELVIKSDKPVLVDFWAEWCGPCRMVGPFVDEIA
                     TEYDGKAIVGKVNVDNNPGISMQFGIRNIPALLYFKDGKVVDKQVGAAPKSVLAGKLD
                     AQLA"
     gene            complement(549..>1115)
                     /locus_tag="PLO59_10840"
     CDS             complement(549..>1115)
                     /locus_tag="PLO59_10840"
                     /inference="COORDINATES: protein motif:HMM:NF013499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HQV01649.1"
                     /translation="SSVTTADLEDMSLYLKREIYLAGIITTANHRIGKTGKRFGIFTL
                     EDLKGTIEIAVFNEKKYLEIERYLKADMFVFIKATVQNKFQRDDDFELSIQNISLLSD
                     IGNQQTKQIITQVSSSQIDDAFIQKFDALIKANTGTIPLKLNIVDNDLKLHVEALPKK
                     AKLVFNTQMFEDLRSLTGDKISLIKNMN"
BASE COUNT          332 a          196 c          185 g          402 t
ORIGIN      
        1 gaaaccaaaa atattatgag gataaatatt caaaaaggat taaataaaaa atgcaggaca
       61 tgcctgcatt ttttatttcg atttggaagt tgattaggcc aactgtgcat ctaacttgcc
      121 tgccaacacc gattttggcg ctgcgccaac ttgtttatca accactttgc catctttaaa
      181 ataaagcaat gcaggtatgt ttctgatacc aaattgcatg cttatgccgg ggttgttatc
      241 tacattaact tttcctacaa tggctttgcc atcatactct gttgctattt catccacaaa
      301 tggaccaacc atgcggcagg ggccgcacca ctcagcccaa aaatctacta atacgggttt
      361 gtctgattta attaccaact cgtcaaagtt ggcgtctgtt aattctaatg ccattttaat
      421 tgggttttaa ttgctttaaa ctgcgttata attttttgta aaactaataa gattgtatag
      481 ttaaatgcta tacgaagttg gcttacaaaa atattaccat atcgggcaat ctgccaatgt
      541 ggctaatttt aattcatgtt ttttataagc gatattttat caccagttaa acttctcaga
      601 tcttcaaaca tttgggtatt aaaaaccaac ttggcttttt tgggtaatgc ttctacgtgt
      661 aatttaagat cattgtcaac aatatttaat tttaatggta tggtgcctgt gtttgctttt
      721 attaatgcat caaatttttg aataaatgca tcatcaattt ggctgctact tacttgtgta
      781 attatttgtt tggtttgttg attgcctata tcacttaaca atgaaatatt ttgtatggat
      841 agttcaaaat catcatcacg ttgaaatttg ttttgcaccg tagctttaat aaaaacaaac
      901 atatcggctt tcagatatcg ctcaatttct aagtattttt tttcattaaa tacggcaatc
      961 tctattgtac cttttaagtc ttctaaagta aaaataccaa agcgtttacc ggttttacca
     1021 atgcggtgat tggctgtagt aattatgcca gctaaataaa tttcgcgctt cagatagagg
     1081 ctcatgtctt caaggtcggc agtggttacg gaact
//
LOCUS       DAOMAA010000930         1114 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_176320_length_1114_cov_3.443815, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000930 DAOMAA010000000
VERSION     DAOMAA010000930.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_176320_length_1114_cov_3.443815"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1114
                     /locus_tag="PLO59_10845"
     CDS             <1..>1114
                     /locus_tag="PLO59_10845"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HQV01650.1"
                     /translation="DFTTGNKNIAAFIKICQQEGVWVLLRPGPYVCAEWDFGGLPPYL
                     LKIPDIKVRCMDTRYMDAVSEYIKHLATEVVSLQCTNGGPILMVQAENEYGSYGNDRV
                     YMDTIRQLWKQNGITVPFYTADGPTAYMLETGSLPGAAIGLDSGSDEKDFNQAKVQNN
                     SVPVFSSETYPGWLTHWGEKWARPDTTALKNEVAFLLQNKKSFNFYVIHGGTNFGFTA
                     GANAASPTQYQPDLTSYDYDAPINEHGAPTAKYYMLRNLITTYTGKKPSAIPAAIKMI
                     ETPDIELKYTGSLWQHLPKAIYTPQPKPLEAFDQNQGLILYRTKLIGHKSGKLKIWEP
                     HDYALVFLNGVFIDTVFRDGGKWDVDLPKTDVKDPVL"
BASE COUNT          355 a          236 c          229 g          294 t
ORIGIN      
        1 gactttacta caggcaataa aaacattgct gcttttatta aaatttgtca gcaagagggt
       61 gtgtgggttt tgctacgacc tggtccttat gtatgtgctg agtgggattt tggcggacta
      121 cctccctatc ttttaaaaat acccgacata aaagtacggt gcatggatac acgttatatg
      181 gatgccgtaa gcgaatatat aaagcacctg gctactgaag tagtttcatt acaatgtact
      241 aacggaggcc ctatactaat ggtgcaagcc gaaaacgaat atggcagcta tggtaatgat
      301 cgtgtatata tggacaccat acgccaattg tggaaacaaa acggcataac ggtaccgttt
      361 tacacagccg atggccccac agcttacatg ctcgaaaccg gtagtttacc cggtgctgcc
      421 ataggtttag atagcggcag cgatgaaaaa gattttaatc aagccaaggt gcaaaacaat
      481 tcagttcctg tattcagctc agaaacctat ccgggctggt taacgcattg gggcgaaaaa
      541 tgggcacggc ccgatactac agctttaaaa aacgaagttg cctttttgct gcaaaataaa
      601 aagtcgttta atttttatgt tatacatgga ggtaccaatt ttggttttac tgcaggcgct
      661 aatgccgcat cgcccacaca ataccaaccc gatttaacca gttacgatta cgatgccccc
      721 attaacgaac atggtgcacc tactgccaaa tattacatgt tgcgtaattt aataacaacc
      781 tataccggta aaaaaccgtc agccataccc gcagctataa aaatgattga aacacccgat
      841 attgaattaa aatatacagg cagcttatgg caacacttac ccaaagctat atacacgcca
      901 caaccaaaac ctttagaagc attcgatcaa aatcagggac tcatattata tcgcaccaag
      961 ttgattggtc ataaaagcgg caagctaaaa atttgggagc cacacgatta tgctttggta
     1021 tttttaaacg gtgtttttat tgatacagta tttcgcgatg gtggcaaatg ggatgtggac
     1081 ctgcccaaaa cagatgtaaa agaccctgta cttg
//
LOCUS       DAOMAA010000931         1113 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_176695_length_1113_cov_2.693762, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000931 DAOMAA010000000
VERSION     DAOMAA010000931.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_176695_length_1113_cov_2.693762"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1098)
                     /locus_tag="PLO59_10850"
     CDS             complement(<1..1098)
                     /locus_tag="PLO59_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01651.1"
                     /translation="MDYTYFKPNGNPTCLCGDWDGMLFFYDYDHSETKTRDTLFAYTK
                     WMWDSIGVKGFRVDAVKNFAPLFAAQTINYLHSNGYIPSVYVGEFYDYNPQSLKSWVD
                     NVKAAMTPAAQQTINPRLFDFALQGSLKEACDNTGYDVRNVFNNGMVDGAGASGFNVV
                     SFVNNHDFRNTSQSCDVDPLLAYAYILTNNAVGLPCVYYPDYYLNAGSYKKAIDDLMD
                     VHKKFIHNTSFREYLNRFNTPFASNFIQGNSDKSLIYQLSGGGSASCIANKDVIVAIN
                     FGNTTLKVDHQINTGNPFHSNVGDTLNDLFGNSNFPYAIILWIPPGKAIQSTACAKPP
                     PEAHPAWQVNRSRGAIVQPVRQPKDPAPAPRP"
BASE COUNT          315 a          228 c          249 g          321 t
ORIGIN      
        1 tgggcgtggc gctggggctg ggtcttttgg ttggcgcact ggttggacga tcgcgccgcg
       61 cgaacggttg acctgccagg ctggatgcgc ctcgggcggc ggcttcgcgc aggcggtgga
      121 ttgaattgct ttgccgggcg gaatccacaa aataatggcg tatggaaaat tggagttacc
      181 aaacaggtcg tttaacgtat cgcccacatt gctgtgaaat ggattgcctg tatttatttg
      241 gtggtcaact tttaaagtgg tgttgccaaa gttaatagcc actattacat ctttattggc
      301 tatacaactt gcactgccgc caccactaag ctgataaatc aaacttttat cgctgttgcc
      361 ttgtataaaa ttagaagcaa agggtgtgtt gaaacggttt aggtattcgc gaaacgatgt
      421 gttatgaata aattttttat gtacgtccat taaatcatca atcgcttttt tataactacc
      481 ggcattgaga tagtaatcgg ggtaataaac acaaggcaaa cccacggcat tattggtaag
      541 tatgtatgca taagctaaaa gcggatctac atcacagctt tgtgaggtgt ttctaaagtc
      601 gtgattgttt acaaatgaaa ctacattaaa gcccgatgcg ccagcaccat ctaccatgcc
      661 gttgttaaaa acattccgca catcataacc tgtattatca caagcttctt taagcgatcc
      721 ctgtaaagca aaatcaaaca aacgtggatt aatagtttgt tgtgctgccg gtgtcatggc
      781 agctttcaca ttatcaaccc aactttttaa actttgtggg ttataatcat aaaattcgcc
      841 aacgtacacc gatgggatat aaccgttact atgtaagtag ttaatagttt gagcagcaaa
      901 cagcggtgca aagtttttta cggcatcaac tctaaaacct ttaacgccaa tactatccca
      961 catccatttg gtataagcaa aaagtgtatc gcgggttttg gtttcggaat ggtcataatc
     1021 gtaaaaaaac aacatgccgt cccaatcgcc acaaaggcat gtagggttac cattgggttt
     1081 aaagtatgtg tagtccatct gaccacggct gct
//
LOCUS       DAOMAA010000932         1112 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_177001_length_1112_cov_3.645222, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000932 DAOMAA010000000
VERSION     DAOMAA010000932.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_177001_length_1112_cov_3.645222"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1112
                     /locus_tag="PLO59_10855"
     CDS             <1..>1112
                     /locus_tag="PLO59_10855"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HQV01652.1"
                     /translation="FSDPLIENKDSKKRYRADVLIEEHIAKIEGKIEKEVEKAAKRFE
                     NFDAAMFRNTNARVLEYQQKIDAINARFKAALENNNLAEVKQIILDLEIVCAISGSRN
                     WTDVRQFNLMFHTQLGSVADEASTIYLRPETAQGIFVNFLNVQKTGRMKIPFGIAQTG
                     KAFRNEIVARQFIFRMREFEQMEMQFFVKPGTELTWYEYWKETRLAWHKALGMPPQKY
                     RYHDHIKLAHYANAACDIEFEFPFGFKELEGIHSRTDFDLKAHEQYSGKKLQYFDPEL
                     NESYVPYVVETSVGLDRMFLATLSTALQTETLDDGTTRDVLKLHPALAPVKAAIMPLV
                     KKDGLPDKAREIMRTLQFDHYCQYDEKDSIGKRYRR"
BASE COUNT          362 a          231 c          233 g          286 t
ORIGIN      
        1 attcagcgat ccattaattg aaaataaaga ttcgaaaaag cgttaccgtg ccgatgtgtt
       61 aattgaagaa catattgcaa aaattgaagg caaaattgaa aaagaagttg aaaaggctgc
      121 caaacgtttc gaaaactttg acgcagctat gtttcgtaac accaatgcac gtgtgttaga
      181 gtaccaacaa aaaattgatg ctataaacgc acggtttaaa gccgcattag aaaacaacaa
      241 cctggccgag gtaaaacaaa ttattttaga cctcgaaatt gtttgcgcca ttagcggtag
      301 ccgcaactgg accgatgtac gccagtttaa tctgatgttt catacacagt taggcagtgt
      361 tgctgacgaa gcatcaacca tttatttacg acccgaaaca gcacaaggta tttttgttaa
      421 ttttttaaac gtgcaaaaaa ccggtcggat gaaaattcct ttcggtattg cacaaaccgg
      481 caaagcgttt cgcaacgaaa ttgtagcacg gcagtttata tttcgcatgc gcgagttcga
      541 gcaaatggaa atgcagtttt ttgtaaaacc cggtaccgaa cttacttggt atgaatactg
      601 gaaagaaacc cgcctggcat ggcataaagc tttaggcatg ccgccacaaa aataccgcta
      661 ccacgaccat ataaaactgg cacattatgc caatgcagct tgcgatatcg aattcgaatt
      721 tccgtttgga tttaaagagt tagaaggcat tcactcccgt accgatttcg acctgaaagc
      781 gcatgaacaa tattcgggca agaaactgca atatttcgac ccggagttaa atgaaagtta
      841 tgtgccttat gtggtagaaa cctctgtagg tttagaccgc atgtttttag caaccttgtc
      901 aacggcactt caaaccgaaa cattagacga tggcaccaca cgcgatgtgc ttaaactaca
      961 tccggctttg gcacctgtta aagcagccat tatgccattg gttaaaaaag atggattgcc
     1021 tgataaagcg cgcgaaatta tgcgcaccct tcagtttgat cattattgcc aatatgatga
     1081 aaaggattcg ataggtaaac gctaccgcag gc
//
LOCUS       DAOMAA010000933         1111 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_177397_length_1111_cov_2.579545, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000933 DAOMAA010000000
VERSION     DAOMAA010000933.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_177397_length_1111_cov_2.579545"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..556)
                     /locus_tag="PLO59_10860"
     CDS             complement(29..556)
                     /locus_tag="PLO59_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQV01653.1"
                     /translation="MPKTAEIQLVTLLKQGDKAAFSTLYDNYSGSLYGIVMRIVNDED
                     LAQDVLQEAMIKVWKNIASYDDTKGTLFTWLLNVTRNLAIDKMRYNSKRQADPIDNVM
                     HKADRAVSHNTEDTIGVKELVANLKPEFKSVIAMAYFNGYTQDEISKELNVPLGTVKT
                     RTRNALLELRKILKA"
     gene            complement(646..>1111)
                     /locus_tag="PLO59_10865"
     CDS             complement(646..>1111)
                     /locus_tag="PLO59_10865"
                     /inference="COORDINATES: protein motif:HMM:NF025167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HQV01654.1"
                     /translation="FLLQPDALSVIKQATVFTVAHCLTLALTMLGYIQPPRQAVEVVI
                     ALSILFIAIENICLNKIYWWRYLIVFLFGLIHGCGFATVLHQTGLPPQATVWALFAFN
                     VGVEVGQIAVIAICYMLIGKLLSNKNWYRQKIVVPISVIIGIVSVYWIIERI"
BASE COUNT          330 a          210 c          183 g          388 t
ORIGIN      
        1 cctgattcta tgtattgctt tggatccact atgcttttaa aattttacgt aattcaagta
       61 atgcgtttcg ggttctggtt ttaaccgtgc ctaacggtac atttaattct ttcgatattt
      121 cgtcttgtgt ataaccatta aaatatgcca tggctattac cgatttaaat tctggtttta
      181 ggtttgcaac caactcttta acacctattg tatcttcggt gttatggctt acagctctat
      241 ctgctttatg cataacatta tcaattgggt cggcttgccg tttactgtta tagcgcattt
      301 tatcaatggc taaatttcgg gttacattaa gcaaccaggt aaacaaggtg ccttttgtat
      361 catcatagct tgcaatattt ttccaaactt ttatcattgc ttcctgtaat acatcctgcg
      421 ctaagtcttc atcatttaca atacgcataa caattccata cagcgaacca ctataattat
      481 cgtacagtgt gctgaatgcc gctttgtcgc cttgtttaag caaggttact aattgtatct
      541 cggcagtttt tggcatgggg tgctaatgca gtacttgatt gttgattttg ttatcgcaaa
      601 tatattttta tttcatttta tcgtcagtag ttatagctaa taattttata ttctttcaat
      661 aatccaataa acggatacaa tgccaattat aacggatata ggaactacga ttttttggcg
      721 ataccagttt ttatttgaca acaatttacc aattaacata taacaaattg caataacagc
      781 aatttgtcct acctccactc ctacattaaa agcaaacaat gcccaaacag ttgcttgtgg
      841 tggtaaaccg gtttgatgca atacagttgc aaaaccacag ccatgtatta atccaaacaa
      901 aaaaacgatg agatagcgcc accagtaaat tttatttaag catatattct caattgctat
      961 aaataatatt gatagtgcaa ttactacttc gactgcttgc ctaggcggtt gtatatagcc
     1021 taacatggtt aatgccaacg ttaaacagtg agctactgta aaaacggtag cttgtttaat
     1081 aacggagagc gcatcgggtt gcagtaaaaa c
//
LOCUS       DAOMAA010000934         1110 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_177688_length_1110_cov_2.670142, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000934 DAOMAA010000000
VERSION     DAOMAA010000934.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_177688_length_1110_cov_2.670142"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1110)
                     /locus_tag="PLO59_10870"
     CDS             complement(<1..>1110)
                     /locus_tag="PLO59_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014781356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HQV01655.1"
                     /translation="AMPGTNNEKIADRSSKLGITWKPISFKTELMSISAEAGVKMRAT
                     VAEFGPVLFSKILELNDTQAGVVSLIFKYCDDNGLLLVDLKDFKKALQFLTNEGKAEI
                     KGEYGSISTATASIILRKIIEIEQQGAEVFFGEKSFDVNDLMQLDENGFGTLNIIRLT
                     DLQAKPKLFSTFMLSLMSEIYATFEEVGDAEAPKLCVFIDEAHLIFNEASKTLLNQLE
                     TMVKLIRSKGVGLYFITQIPTDVPASILSQLGLKVQHALRAFTAQDRKVIKATAENYP
                     ETVFYQTDELLTQVGIGEALVTVLNEKGNPTPLAHTMLCAPATRMDVLTPDEINNLVA
                     KSALVKKYNQTIDAESAYEMLAKKIEAMTDETDIA"
BASE COUNT          318 a          230 c          196 g          366 t
ORIGIN      
        1 tcggcaatgt cggtttcatc agtcattgct tcaatttttt tggccaacat ttcgtaagcg
       61 ctttcggcat caattgtttg attgtatttt ttaaccaatg ctgatttggc aactaaatta
      121 tttatctcgt caggtgttaa aacatccatg cgcgtggccg gtgcacaaag catggtatgg
      181 gctaatggcg ttgggtttcc tttttcattt aaaacagtaa ccaaagcttc gccaatgcct
      241 acttgtgtaa gtaattcatc ggtttgataa aaaacggttt caggatagtt ttcggctgtg
      301 gctttaataa ccttgcggtc ctgggctgta aatgcacgta aggcatgttg cacttttaaa
      361 cctaactggc ttaatatact ggcaggcaca tcggttggta tttgtgtaat aaaatacaaa
      421 cccacgcctt tacttctgat tagtttaacc atggtttcta actgattgag caaggtttta
      481 gaagcttcgt taaaaatcaa atgtgcctca tcaataaaaa cacaaagctt gggcgcttca
      541 gcatcgccta cctcttcaaa tgttgcgtaa atttcgctca tcaggcttag cataaaagtg
      601 ctaaacagtt tgggcttagc ttgcaaatcg gtaagtctga taatgtttaa ggtaccaaac
      661 ccattttcat ctaattgcat taaatcattt acatcaaacg atttctcgcc aaaaaaaact
      721 tcggcacctt gctgttcaat ttctattatt ttacgaagta ttatggatgc agttgctgta
      781 cttatactgc catactcgcc cttgatttcg gctttgcctt cattggttaa aaactgaagt
      841 gcttttttaa aatcttttaa atcaaccaac aacaaaccat tatcatcaca atatttaaaa
      901 atcaacgaaa ccacaccggc ctgtgtatca tttaattcta aaattttcga aaataaaacc
      961 ggaccaaact cagcaacggt agcacgcatt tttacaccgg cttcggccga tatagacatc
     1021 agttctgttt taaaggaaat aggtttccag gtaataccca gcttactgct tctatcggca
     1081 attttttcat tgtttgtacc cggcatagct
//
LOCUS       DAOMAA010000935         1107 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_178582_length_1107_cov_4.026616, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000935 DAOMAA010000000
VERSION     DAOMAA010000935.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_178582_length_1107_cov_4.026616"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="PLO59_10875"
     CDS             complement(<1..226)
                     /locus_tag="PLO59_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01656.1"
                     /translation="MAENSSMMRDLTKSALTTAIGVVVTYFVTSAIKKDEYKQNDIEK
                     EKQRMEALEKTNKLLADYVGRYDSLHTRYIN"
     gene            366..>1107
                     /locus_tag="PLO59_10880"
     CDS             366..>1107
                     /locus_tag="PLO59_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008147337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HQV01657.1"
                     /translation="MLRTHTCGQLNISDVGTTVTLCGWLQRARDLGGMTFIDLRDRYG
                     ITQLAFNMVTDAALLADARKLSREYVIQVTGIVIERSSKNLKVPTGEIEIEVKTLNVL
                     NAAKLPPFTIEDDTDGGDDIRMQFRYLDIRRNAVKQNLLLRHKVCQLTRSYLDQQGFI
                     EIETPVLIKSTPEGARDFVVPSRMNAGEFYALPQSPQTFKQLLMVAGMDKYFQIVKCF
                     RDEDLRADRQPEFTQIDCEMSFIEQDDIL"
BASE COUNT          324 a          215 c          229 g          339 t
ORIGIN      
        1 aattaatata acgcgtatgc aaactatcgt aacggcctac atagtcggcc aataacttat
       61 tggttttctc caaagcctcc atgcgttgtt tttccttttc aatatcgttt tgtttgtatt
      121 cgtccttttt aatagccgat gtaacaaagt aggttacaac cacaccaatt gctgtggtta
      181 aagcgctttt ggttaaatcg cgcatcatgc ttgagttttc tgccatgtgg taaggtgtta
      241 gttgcgatgc aatataattc agcgattata aagtacacca ttacaaacac ttttttattg
      301 atgtagttta tcgcttatgc tttacgtaaa gccctatatt cgcgccctca aaaaaatata
      361 gttttatgtt acgtacgcat acttgtggtc agttaaatat tagcgatgtt ggtacaaccg
      421 ttaccttatg cgggtggttg caacgcgcac gcgatttggg aggtatgacg tttattgatt
      481 tgcgcgatag gtatggcata acccagcttg catttaatat ggttaccgat gctgcattac
      541 tagccgatgc acgtaagctt tcgcgcgaat atgtgataca ggttacaggt attgttattg
      601 aacgcagcag taaaaacctg aaagtgccaa caggtgaaat cgaaattgaa gttaaaacac
      661 taaatgtttt aaatgcagcc aagttgccac catttactat tgaagacgat accgatggcg
      721 gtgatgatat acgcatgcaa ttccgctatt tggatatcag acgaaatgcc gtaaaacaaa
      781 atcttttgtt gagacataag gtatgccagc taacccggtc ttatttagac caacaaggct
      841 ttatcgaaat agaaactccg gtactcataa aatcaacacc cgaaggcgca cgcgattttg
      901 tagtaccatc acgtatgaat gccggtgagt tttatgcatt gccacaatcg ccacaaacgt
      961 ttaaacagtt actgatggtg gccggtatgg ataagtattt tcaaattgta aaatgcttcc
     1021 gcgatgaaga cctgcgtgcc gaccgtcagc ccgagtttac acaaatagat tgtgaaatga
     1081 gttttattga gcaagacgat attttac
//
LOCUS       DAOMAA010000936         1107 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_178589_length_1107_cov_3.684411, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000936 DAOMAA010000000
VERSION     DAOMAA010000936.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_178589_length_1107_cov_3.684411"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1086)
                     /locus_tag="PLO59_10885"
     CDS             complement(<1..1086)
                     /locus_tag="PLO59_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01658.1"
                     /translation="MWKGIVLEPGATLETAFYPLIEEAQIAVNSLPGATVMCNDHTTF
                     NANNQSIILNFDAASPAYTGFIYQTKFECNQQSNGNPITLIAPYHTQIPQTGIQINDA
                     AFVKIGNENDVAIPPLYQNLFNNLNCGIHSNNSTVEIYNNKFSNIYNAQPEYVSSGYG
                     IYIRPNQLQTNFIAIVGGANMGNTTRENVFYNCNNGIYMRKNIDCFINNNTFTNIMEQ
                     PGTSANARGSGKGIEAFNATGSNLIVTENRFNNFNQGIYMSKLEDCYLYFHYNTFNYL
                     GNSATIGTSAITISNPLATSYSQPMDVQYNLIKQCRKGISISKFENVQVHDNTILFAQ
                     ADIAAHPSDAYKGILIENSAPAGNTVKS"
BASE COUNT          319 a          190 c          223 g          375 t
ORIGIN      
        1 gctctttacg gtgttgcctg ccggggcact gttttcaatc aatattcctt tgtaggcatc
       61 acttggatgc gcggctatat cggcctgagc aaataaaata gtattatcgt gtacctgtac
      121 gttttcaaat ttagaaatag aaattccttt gcgacattgt ttaataagat tgtactgtac
      181 atccatcggt tggctgtaag aggtggctaa ggggttggaa attgttattg ccgatgtacc
      241 aatagtggca ctgttgccca aatagttaaa cgtattgtag tgaaagtata aataacaatc
      301 ttcgagtttg ctcatataaa taccctgatt aaaattatta aaacgatttt ctgtaacaat
      361 taaatttgaa cccgttgcat taaatgcctc aatacctttg ccgctaccac gtgcattagc
      421 ggaggtaccc ggctgttcca taatatttgt aaaggtgttg ttgttaataa aacaatctat
      481 gtttttgcgc atgtaaatgc cattgttgca attataaaaa acattttcgc gggtagtgtt
      541 tcccatatta gcgccaccca caatagcaat aaaattggtt tgtaactgat tgggtcttat
      601 ataaatccca taacctgacg aaacatactc cggttgtgca ttataaatat ttgaaaactt
      661 attattatat atctcaacgg tgctgttatt ggagtgtatg ccacaattaa ggttattaaa
      721 aaggttttga tataatggcg gaatggcaac atcattttcg ttaccaattt ttacaaaggc
      781 cgcatcattt atctgaatac ctgtttgcgg tatttgtgta tgataaggcg ctattaaggt
      841 aatgggattg ccattgcttt gttggttgca ttcaaatttt gtttggtaaa taaaacctgt
      901 ataagctggc gatgcagcat cgaaatttaa aattatactt tggttattgg cgttaaaggt
      961 ggtatggtcg ttacacatta ctgttgcacc cggtaagctg tttacagcaa tttgtgcttc
     1021 ttcaataagc ggataaaaag cggtttcaag cgtagcacct ggttctaaca caatgccttt
     1081 ccacatatag ttgccatcgc aggtgtg
//
LOCUS       DAOMAA010000937         1105 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_179198_length_1105_cov_2.998095, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000937 DAOMAA010000000
VERSION     DAOMAA010000937.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179198_length_1105_cov_2.998095"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1086)
                     /locus_tag="PLO59_10890"
     CDS             complement(<1..1086)
                     /locus_tag="PLO59_10890"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: protein motif:HMM:NF016438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HQV01659.1"
                     /translation="MARYTQRAQHQPIPALPVNYSHFYTPFEHQRKTSNTCINIGSQQ
                     VPRVIGVLNQTALNGKPIQQKTLFPFGYSYSVALDKWHLADQACDYIYTGSLPINFEI
                     PGTLGIIYNYDLWLKQNKQRCYPLLNIQHYESATVLHQQLNFVTVTIHTLEGILKNNP
                     RPGLVLIFETENLHGMAEMREMVLHCMANTAWQHIPIIFKRSYQNLHTSTLQLHAATD
                     LGALLIDGFGDGICIDANNTTAGPNTINALAFGILQASRLRISKTEYISCPSCGRTLF
                     DLQETTARIRSRTEHLKGVKIGIMGCIVNGPGEMADADYGYVGVGVGKITLYKGKEVV
                     KRNIDTHLAVDELINLIKQHGDWVEKET"
BASE COUNT          291 a          216 c          241 g          357 t
ORIGIN      
        1 ggtttctttc tcaacccaat cgccatgttg ctttatcagg ttaattaatt cgtcaacggc
       61 caggtgtgta tcaatatttc ttttaaccac ttctttacct ttgtaaagtg taatttttcc
      121 tacacctaca ccaacgtagc catagtcggc atcggccatt tcgcccgggc cgtttacgat
      181 gcaacccatg ataccaattt ttacaccctt taaatgttcg gtacggctac gtatgcgtgc
      241 tgtggtttct tgcaaatcga aaagtgtacg gccacaactt gggcagctta tatattcggt
      301 tttgcttatg cgcaaacgcg aagcctgtaa aatgccaaac gccaatgcat taatggtatt
      361 aggaccggca gtggtgttgt tggcatcaat acaaatacca tcaccaaaac catcaataag
      421 caatgcgcct aaatcggttg ctgcatgtaa ttgtaaagtg gaggtgtgta aattttggta
      481 actacgttta aaaataattg gtatgtgttg ccaggctgta tttgccatac agtgcaaaac
      541 catttcacgc atttcggcca tgccatgtaa attttcggtt tcgaaaatta ataccagccc
      601 gggcctgggg ttgtttttta aaatgccttc caatgtatgt atggtaacag ttacaaaatt
      661 taattgctga tgcaaaaccg ttgcactttc atagtgctgt atgttaagca atgggtaaca
      721 acgctgttta ttttgtttta gccaaaggtc gtaattgtaa ataataccta aggtgcccgg
      781 tatttcgaag ttaatgggga ggctacctgt ataaatataa tcgcaggcct ggtcggctaa
      841 atgccatttg tctaatgcaa cactataact gtaaccaaaa gggaacagtg ttttttgttg
      901 aatgggtttg ccatttaaag ccgtttggtt taatacacca attacacgcg gtacctgttg
      961 gctgcctatg tttatacagg tatttgacgt tttgcgttga tgttcgaacg gtgtatagaa
     1021 atggctgtag ttaactggta atgccggtat gggttggtgt tgtgctcttt gtgtgtaacg
     1081 tgccaccaat gctttggcaa caggt
//
LOCUS       DAOMAA010000938         1105 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_179220_length_1105_cov_2.800000, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000938 DAOMAA010000000
VERSION     DAOMAA010000938.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179220_length_1105_cov_2.800000"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..>1105
                     /locus_tag="PLO59_10895"
     CDS             85..>1105
                     /locus_tag="PLO59_10895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HQV01660.1"
                     /translation="MSKKWKISLVAIALSVSSFAWFSFSDDYFEVSRNLDIFTSLYRE
                     LNVYYVDETKPGSLMKTGIDAMLKSLDPYTNYIPESDIEDYRFMTTGQYGGIGALIAK
                     KGDQIVITDPYENYPAQKAGLLAGDVVLEVDGKELNGKNSDDVSKALKGQPGTSVRLT
                     IKRDGTLKPFEKTLLREEIKIPAVSYSGVMGDGSVGYIRLTSFTENCGQEVKDAFVKL
                     KTENNIKSLVLDLRENPGGLLNEAVNIVNLFVPKGTMVVKTHGKNKEWDKEYKALNNP
                     VDTEMPLAVLINSGSASASEIVAGCIQDLDRGILIGQRSFGKGLVQTTRPISYNAQLK
                     VTTAKY"
BASE COUNT          356 a          206 c          252 g          291 t
ORIGIN      
        1 ccattaaacg attggcaacc tgccatccct ggggtggcag tggctacgat ccggttcagt
       61 aaaatttaat caataaagaa tactatgtca aaaaaatgga agattagttt agtggcaatt
      121 gccctgtcgg ttagcagttt tgcctggttt agttttagtg acgattattt tgaggtatcg
      181 cgcaacctcg acatttttac atcactttac cgcgaactga acgtgtatta cgttgacgaa
      241 accaaaccgg ggtcgcttat gaaaaccggc attgatgcca tgcttaaatc actcgaccca
      301 tatacgaact acatacccga aagcgatatt gaagactatc ggtttatgac tacaggtcaa
      361 tatggtggta ttggtgcatt aattgcaaaa aaaggcgatc aaatagttat taccgatcct
      421 tatgaaaact atccggcaca aaaagccggg ctattagccg gtgatgttgt gttagaagta
      481 gatggcaaag aattaaacgg caaaaacagt gatgatgtaa gcaaagcact taaaggccaa
      541 ccgggcacat cagtgcggtt aaccattaaa cgcgatggca ctctaaagcc attcgaaaaa
      601 acactgctgc gtgaagagat aaaaataccg gctgtttctt atagtggtgt tatgggcgat
      661 ggtagtgtag gttatatcag gcttacaagc tttaccgaaa attgtggtca ggaggtgaaa
      721 gatgcatttg taaaattaaa aaccgaaaac aacatcaaat ctttagtact cgatttgcgc
      781 gaaaaccccg gaggactgct taacgaagcc gttaacattg ttaatctgtt tgtgcccaaa
      841 ggtaccatgg tggttaaaac acatggcaag aataaagagt gggataaaga gtacaaagct
      901 ttaaacaacc ctgttgatac tgaaatgccc ttggctgttt taattaacag tggttcggca
      961 tcggcatcag agattgttgc aggttgcatt caggatttag acagaggtat tttaatagga
     1021 caacgttcgt ttggcaaggg gttggtacaa accacacggc ctatcagtta caatgcgcag
     1081 ttaaaagtaa ctacagccaa atact
//
LOCUS       DAOMAA010000939         1105 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_179354_length_1105_cov_1.909524, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000939 DAOMAA010000000
VERSION     DAOMAA010000939.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179354_length_1105_cov_1.909524"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1105)
                     /locus_tag="PLO59_10900"
     CDS             complement(<1..>1105)
                     /locus_tag="PLO59_10900"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV01661.1"
                     /translation="EKVTCAFSFNDITIDSLTGCAGPGSENCFTIHYNGSCTNVTMQI
                     RYFKWLLNTEVTIPIAGNGTTFSVCAYDTMYLTSKGVMLFGTDGSNVDSLYFKPNRIK
                     CNRNFTYNVSYDQCGAQISGVNYPSGHCNPLDTWLKLKKISDNNVDFDYYYDIASLPN
                     GTYLIGASDAGCNSKIKTLVISGAVKEAPTGLSVRQVDGMNKVKIRWKDECPYYSTFD
                     VELYQIQIRPHGSGTWQVYKIAQPNYSYPHYYTIIDSISLGVEYDYRIRTWWAGDKSK
                     WSSIRHFCFGGGCAARTVSENDVNENNVALTVYPNPANDVINFNFEDAVENQVVYTIY
                     NQLGKLVMTNNTHVNSDAMQLNVSSLNNGLYFIA"
BASE COUNT          334 a          222 c          195 g          354 t
ORIGIN      
        1 gcaataaaat acaaaccatt gtttaatgat gatacgttta actgcatggc atcgctgttt
       61 acatgcgtat tatttgtcat caccaatttg cctaactgat tgtaaatggt ataaacaact
      121 tggttttcaa ctgcatcttc gaaattaaaa tttataacat cgttcgcagg gttgggataa
      181 acagttagtg ctacattgtt ttcattaaca tcgttttctg aaacagtgcg tgctgcacag
      241 ccaccaccaa aacaaaagtg tcttatcgat gaccatttac ttttatcgcc agcccaccac
      301 gtgcgaatac ggtaatcata ttcaacacct aaactaatac tatcaattat cgtatagtag
      361 tgtggatacg aataattagg ttgtgctatt ttgtacactt gccatgtacc actaccatgt
      421 ggtcttattt gaatttgata taattcaaca tcaaatgtgc tatagtaagg gcactcatct
      481 ttccaacgaa ttttaacttt attcatacca tccacttgcc ttaccgataa accggttggc
      541 gcttctttaa cagcacccga aattaccaag gtttttattt ttgaattgca gccggcatcg
      601 cttgcaccaa taagataagt tccattaggt agggaggcaa tgtcgtagta ataatcaaaa
      661 tcaacgttat tgtctgaaat cttcttgagc tttagccagg tatcgagcgg attgcaatgc
      721 ccactggggt aatttacacc tgagatttgg gctccgcatt gatcgtaaga aacattgtaa
      781 gtaaaattac gattgcattt tatccgatta ggcttaaagt ataaactatc aacgttcgat
      841 ccatcggtgc caaatagcat tacgccctta gatgtcaaat acattgtatc gtatgcacat
      901 acactaaagg ttgttccatt gcccgcaata ggtatggtaa cttctgtatt taacaaccat
      961 ttaaaatatc ttatttgcat ggtaacattg gtgcatgagc cattataatg gattgtaaaa
     1021 caattttcgc ttccgggccc ggcacaaccc gttagcgagt caatggttat atcattaaaa
     1081 ctaaaggcac aggttacttt ttcgg
//
LOCUS       DAOMAA010000940         1105 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_179356_length_1105_cov_1.896190, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000940 DAOMAA010000000
VERSION     DAOMAA010000940.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179356_length_1105_cov_1.896190"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="PLO59_10905"
     CDS             <1..509
                     /locus_tag="PLO59_10905"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HQV01662.1"
                     /translation="RATLMLAFFTAIVCATKRVYITTPYLIPNESIVTALKQAVLSGL
                     DVRLLVPKNSDSFFVNAASQSFYNELLDAGVKIYQYYKGFIHAKTVAIDNNLAIVGTA
                     NMDIRSFDLNFEINAFVYNETLNKQLTDCFLEDLKHSTLIDKHAWKKRKKRYRLVESV
                     ARLVAPLL"
     gene            complement(559..>1105)
                     /locus_tag="PLO59_10910"
     CDS             complement(559..>1105)
                     /locus_tag="PLO59_10910"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV01663.1"
                     /translation="GFIAENVAQVFPELVKDATSAEIIGKDRKVNHPSVAFKAVNYTG
                     LIPVVVEAIKEQQKLIDAKDVLLANQQAQIESLQNQLNEIKDLLVTEKYGNINAQSSL
                     AQNVPNPAKGTSSIAYMLPQNKSGIITIVDLKGNVLETFQVNQSGQVEISTSKYQAGS
                     YTYTLVIEGKVISSRTMVIVK"
BASE COUNT          347 a          194 c          190 g          374 t
ORIGIN      
        1 aacgtgccac cttaatgctt gcatttttta cagccatagt atgtgccacc aaaagagtat
       61 atataactac accatatcta atacctaacg aaagcattgt aacagcttta aagcaagcgg
      121 tgttaagcgg tttagatgta aggttactgg taccaaaaaa ttcggattcg ttttttgtta
      181 atgcagcttc gcaatctttt tacaatgaat tactggatgc tggtgtaaaa atatatcagt
      241 actataaagg ttttatacat gcaaaaacag tggctattga taataacctt gccatagttg
      301 gaacagctaa tatggacatc agaagctttg atttgaattt tgaaattaat gcatttgttt
      361 acaatgaaac actgaataaa caattaaccg attgcttttt ggaagattta aaacacagca
      421 ctttaattga caaacatgca tggaaaaaaa ggaagaaaag atatcggtta gttgagtcgg
      481 tagcacgttt agtggcaccg ttattataaa aaaaaactcc ggctattgcc ggagtttttt
      541 tcgtgtaaaa tgcccatgtt acttcactat aaccatagta cggctactta taactttacc
      601 ttcaataact aaagtataag tataactgcc ggcttggtat ttagatgtac taatttcaac
      661 ctgacccgat tggtttactt gaaaagtttc taaaacatta ccttttaaat ctacgatggt
      721 aataataccc gatttatttt gtggtaacat gtaagcaata ctactggtac cttttgccgg
      781 attgggaaca ttttgagcta agctgctctg cgcatttata ttaccgtatt tttcagtaac
      841 aagcaggtct ttaatttcgt ttaactgatt ttgcaatgac tctatttgtg cttgctgatt
      901 tgctaacaaa acatctttag catcaatcag tttttgttgt tccttaattg cttcaacaac
      961 aacaggaatc aggcctgtgt agtttacggc tttaaatgca accgatggat gatttacttt
     1021 tctgtctttt ccaataattt cggccgaggt tgcatctttt accaactccg gaaaaacttg
     1081 tgctacattt tcggctataa aacct
//
LOCUS       DAOMAA010000941         1104 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_179560_length_1104_cov_2.591039, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000941 DAOMAA010000000
VERSION     DAOMAA010000941.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179560_length_1104_cov_2.591039"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            106..1071
                     /locus_tag="PLO59_10915"
     CDS             106..1071
                     /locus_tag="PLO59_10915"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HQV01664.1"
                     /translation="MKKPIITILLYASCLQLFAQQAPDTTNKIIEFKEAVISVNKVEE
                     KRKEVVQQFSTINAKQIKTLNAQNTAELIMLTGNLNVQTSQQCGGSPAIRGFEASRIL
                     LTLDNVRMNNLIYRSGHLQNILTFDQNVFERVDILYGPSSTVYGSDALGGVIHMHTRN
                     PQLSNDKLLLNGNAYARYGSINNEKTGHLDLNIAGKRFGSLTSVTYSDMGDQKSGRNK
                     LFQDSVFGMRPYYIDRIEGKDSIVANNDKWMQKFSGYNQVDVLQKFLFKQNKHLTHVV
                     NLQYSTSSDIPRYDRLTELSGNGDLAFAEWYYGPQTRLLAAYEMH"
BASE COUNT          382 a          201 c          209 g          312 t
ORIGIN      
        1 taaaataact caggcacacc aaaattaaat ccctaattat tatacaaaag cagctttgta
       61 aaatggcatt gctgtgcttt tgtattaacc catattttat attcaatgaa aaaaccaatc
      121 ataactatat tattatacgc aagttgtttg caattgtttg cacaacaagc accagataca
      181 accaataaaa ttatcgaatt taaagaagct gtaatttctg taaataaggt tgaagaaaag
      241 cgcaaagaag tagtgcagca atttagtacc attaatgcta aacagataaa aaccctcaat
      301 gcgcaaaaca cggcagaatt aatcatgcta accggtaatt taaatgtgca aaccagccag
      361 caatgtggcg gaagcccggc tatccgtggt tttgaagcaa gtcgtatatt actcacgtta
      421 gataatgtac ggatgaataa tttaatctat cgttcgggcc atctccaaaa tatactcact
      481 tttgatcaaa atgtttttga acgggttgat atattgtatg ggccatcgag tacggtatat
      541 ggaagcgatg ctttgggtgg tgttatacac atgcatacgc gcaacccgca attatcaaac
      601 gataaattac tacttaatgg caatgcctac gcacgttacg gaagtattaa caatgaaaaa
      661 accggccacc tcgatttaaa tatagccggc aaacgttttg gttctctcac ttcggtaact
      721 tatagcgaca tgggtgatca gaaatcgggg cgaaacaaat tgtttcagga ttcggttttt
      781 ggtatgagac cttattacat cgaccgtatc gaaggcaaag acagtatagt ggccaacaac
      841 gataaatgga tgcaaaagtt tagcggatat aatcaggtag atgttttgca gaaattttta
      901 ttcaaacaaa acaagcatct tactcacgta gtaaatttgc aatacagtac ttcgtctgat
      961 atacctcgtt atgaccggtt aaccgaattg agtggaaatg gggatttggc atttgccgaa
     1021 tggtactatg gaccgcaaac acgtttgctt gccgcatacg aaatgcatta acaaaatcag
     1081 gaacaatttt ttcaaaacat taat
//
LOCUS       DAOMAA010000942         1103 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_179856_length_1103_cov_3.418893, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000942 DAOMAA010000000
VERSION     DAOMAA010000942.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179856_length_1103_cov_3.418893"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..641)
                     /locus_tag="PLO59_10920"
     CDS             complement(123..641)
                     /locus_tag="PLO59_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016197431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HQV01665.1"
                     /translation="MKKIISALIIITMQTQAQNIYDFKFKSIDGKTINLSDYSGKKIL
                     IVNTASKCGYTNQYEDLEKLNKDYADKLVVIGFPANNFGAQEPGTNDEIDAFCKNNYG
                     VTFIVSEKISVMGADMHPLFKWLTTTQNPNFTGDIEWNFEKFLIDEKGQLIHRYRSAV
                     KPLSSEITSAIN"
     gene            912..>1103
                     /locus_tag="PLO59_10925"
     CDS             912..>1103
                     /locus_tag="PLO59_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01666.1"
                     /translation="MATINLANAQYGQRVYFAGGAVKESFNDGLYSTVNLNGGLPTYV
                     ATGISDSTGNLYTRFTRMAS"
BASE COUNT          351 a          173 c          164 g          415 t
ORIGIN      
        1 taacaaatag gcaaccttag tttatgtgcc aagcaaaaag tttaccctac tgcgattact
       61 ttattaggat gtttaaccat tttggaagct aatttctatg gggttaaaaa cacctgcatt
      121 acctaattta ttgccgatgt aatttcgcta cttaatggtt taactgcact gcgataacgg
      181 tgtatgagtt ggcctttttc atcaattaaa aatttttcga aattccattc tatatcgcct
      241 gtaaaatttg ggttttgtgt ggttgttagc catttaaata atggatgcat atcagcaccc
      301 attaccgata ttttttcact tactataaat gtaacaccat aattattttt acaaaatgca
      361 tcaatctcat cattagtacc aggctcctgt gcaccaaaat tattggccgg aaatccaata
      421 accactaatt tatcagcata atctttgttt agtttttcaa ggtcttcgta ttgattggta
      481 tagccacatt tagatgcagt gtttacgatc aatatttttt tgccactata atctgatagg
      541 tttattgttt taccatcaat tgatttgaat ttgaaatcgt aaatattttg tgcttgcgtt
      601 tgcatggtaa taattattaa agcggatatg atttttttca tactatgtgt tttacaatat
      661 aaactttaac tgcttagtat tttctgcctc atatttttta aaatcgaaat ccaatagctt
      721 ttgttcataa ttcaacaagt caaatctatt gatttcaata aaaccgaata atttactttt
      781 aatatatttt aaagaaggat aaataaattg tttaaaaata aattgtgtag gatttaatta
      841 tttaatattt ttagatgcac aaacaattaa tacctcaaat tatgaaaaaa cttctactat
      901 gtttaagttg catggcaaca attaatttgg caaatgcaca atatggtcaa cgtgtttatt
      961 ttgcaggtgg tgcagttaaa gaatccttta atgatggact ttactcaacg gtaaatctca
     1021 atggcggttt accaacttat gtcgcaaccg gaatttctga tagtaccggg aatttatata
     1081 cccgctttac acgtatggca agt
//
LOCUS       DAOMAA010000943         1103 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_179922_length_1103_cov_2.539122, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000943 DAOMAA010000000
VERSION     DAOMAA010000943.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179922_length_1103_cov_2.539122"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(318..824)
                     /locus_tag="PLO59_10930"
     CDS             complement(318..824)
                     /locus_tag="PLO59_10930"
                     /inference="COORDINATES: protein motif:HMM:NF019241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HQV01667.1"
                     /translation="MEQLFLTESMHHLNTYMQRIETACAALSEAEIWWRPHQRALSIG
                     TTLLHLNGNITQWVISGLYGQPDNRNRAAEFDNSLQPEKLFLLEQLKQTIGKANTVII
                     NLNEAKLMSAYSIQGFETTGLQATYHVVEHLSWHCAQITYISKLLQGESFNFSFYNTE
                     ALNNSKNK"
     gene            908..>1103
                     /locus_tag="PLO59_10935"
     CDS             908..>1103
                     /locus_tag="PLO59_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RimJ/RimL family protein N-acetyltransferase"
                     /protein_id="HQV01668.1"
                     /translation="MISINDTLKLVLPDLNMAEPLFQLIIKNKMHLRPWLNWLDKATD
                     VSFTKNFITASLLQYSNGQSI"
BASE COUNT          332 a          199 c          192 g          380 t
ORIGIN      
        1 tatattatgg caggtttgtg aaggagtgat tagtggtttg ttgttagcga taagtagtta
       61 gttgttagtg ggttgcattt gctttggttt gtttgtaaac tatctccgtt tgtagcttca
      121 gtaatcaatt tgtccaacgt gcattgcgtt ggtaattgca tgtgaaaaat cttatccgta
      181 aaaatcctta attatacttt atgtctttag gcacttaaaa gacaaaatat aaaaaatagc
      241 cttacggtta tcgagcataa accagatgct ttcctatcct ttcatatttg tattaaccga
      301 atgccaaacc ggtcaaacta tttattctta ctgttattta aagcctccgt attataaaat
      361 gaaaagttga aactttcgcc ttgcaacaat ttactgatgt atgttatttg tgcacaatgc
      421 cagcttaaat gttcaactac atgataagtt gcttgcaaac ctgtagtttc aaatccctga
      481 atactatagg ctgacatgag ttttgcttca ttcaaattta taataaccgt attggcttta
      541 ccaatagttt gcttcaactg ttccaataaa aataattttt cgggttgcag gctattgtca
      601 aattcggcag cacggtttct gttatcgggt tgcccgtaaa gcccacttat tacccactgt
      661 gtgatgttac cattcagatg cagtaaagta gttcctatgg ataatgcgcg ctgatgtgga
      721 cgccaccaaa tttcagcttc gcttaaagcc gcacatgcgg tttcgatacg ctgcatgtac
      781 gtatttaaat ggtgcatcga ctccgttaaa aatagctgtt ccatacattt gttttaatgg
      841 tgatgataat caatttttac aacgaaaaat tattcaagct ttaatcacta ttaaaaccct
      901 aacataaatg atttctatca acgatacact aaagttggtg ttgcccgatt taaacatggc
      961 cgagcctttg tttcagttaa ttataaaaaa caaaatgcac ttaaggccct ggcttaactg
     1021 gttagataaa gcaacagatg tatcatttac caaaaacttt attactgcat ctttacttca
     1081 atatagcaat gggcaaagca ttc
//
LOCUS       DAOMAA010000944         1102 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_180192_length_1102_cov_3.404011, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000944 DAOMAA010000000
VERSION     DAOMAA010000944.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_180192_length_1102_cov_3.404011"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..704)
                     /locus_tag="PLO59_10940"
     CDS             complement(150..704)
                     /locus_tag="PLO59_10940"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HQV01669.1"
                     /translation="MNWLDGIIALPLLWGAYQGFKKGIEFMLLMIIGMVLGMYIAFKF
                     SGLIVGFLANHISATAALPYIAFALVFIIIVLVVYLLSKFLEAILKTTALTGLNKAAG
                     AMAGLTKWALIVSVFLWMFKALEPHVYVIPLKAQDESITYNPVLKLATFITPAFTDIK
                     RVFNNNIGKVDSLIINHVPDSTLK"
     gene            complement(729..>1102)
                     /locus_tag="PLO59_10945"
     CDS             complement(729..>1102)
                     /locus_tag="PLO59_10945"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HQV01670.1"
                     /translation="GHLNYASRLELMHLLFGVAAADNHLDKTELDTLSQLAYELGIKQ
                     PDFISLQAMFVVDKTAAYKILEIDETATDDEVKKAYRKMAVKYHPDKVHHLGPDFQKD
                     ANIKFQKITEAYESIKKQRGF"
BASE COUNT          373 a          196 c          173 g          360 t
ORIGIN      
        1 agtaaaataa attgtaagtg ttttttcatt ggaggtatat tttagggaaa atggaactta
       61 gcttagtaag taacaatgat atataaaaag ttgcataccc ggcaacaaac cagcaataaa
      121 cttacttgtg atttaatccg agaacttttt tattttagag tgctatcggg cacatgatta
      181 ataataaggc tatcaacctt gccaatatta ttattaaaca cacgcttaat atctgtaaat
      241 gccggtgtta taaatgtggc taattttaaa accggattat aagttatcga ttcatcctgt
      301 gcttttaatg gaattacata cacatgtggc tcaagtgctt taaacatcca taaaaaaaca
      361 ctaacaataa gtgcccattt ggttaaaccg gccattgcac cagcagcctt atttaaaccg
      421 gtaagtgcag tggtttttaa tatagcttct aaaaattttg atagcaggta aacaactaaa
      481 acaataataa taaaaaccaa tgcaaatgct atataaggca atgctgctgt tgcactaata
      541 tgattggcta aaaagccaac aattaaacct gaaaatttaa aggcaatata catacccagc
      601 accattccaa taatcatcaa caacataaac tcgatgccct ttttaaaacc ttggtatgca
      661 ccccataaca gcggtagtgc aataatgcca tctaaccaat tcatagtatt gtttgtttat
      721 ttttaaaatc aaaagccgcg ttgctttttt attgattcgt atgcttcggt aattttctga
      781 aatttaatat tagcatcttt ctgaaagtcg ggtcctaaat ggtgtacttt atccggatga
      841 tatttaacgg ccattttacg atatgctttt ttcacttcat cgtctgttgc tgtttcatca
      901 atctctaaaa ttttataggc cgctgtttta tctacaacaa acattgcttg taatgaaata
      961 aaatcaggtt gctttattcc taactcatag gccagttgcg aaagtgtatc aagttcggtt
     1021 ttatctaaat gattatcggc agcggctaca ccaaaaagca aatgcattaa ttctaagcgt
     1081 gatgcataat taagatgccc ct
//
LOCUS       DAOMAA010000945         1101 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_180496_length_1101_cov_3.056405, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000945 DAOMAA010000000
VERSION     DAOMAA010000945.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_180496_length_1101_cov_3.056405"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            288..629
                     /locus_tag="PLO59_10950"
     CDS             288..629
                     /locus_tag="PLO59_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01671.1"
                     /translation="MVHYKSDRSFVHVGAGWRYADAPKNKLSYKAKPETNTAPYFLNT
                     NSFDAQNAQAYMFELIGTKGPLSLISEYMLQIAHTPESKQSLIIGNVLLVGLLQAKTG
                     NTISRTAMLAN"
     gene            complement(920..>1101)
                     /locus_tag="PLO59_10955"
     CDS             complement(920..>1101)
                     /locus_tag="PLO59_10955"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611664.1"
                     /note="Catalyzes the oxidation of dihydrolipoamide to
                     lipoamide; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HQV01672.1"
                     /translation="LGVHMIGPRCADLIAEAVVAMEFRASAEDITRMSHAHPTYTESF
                     KEACLAACGSGAIHL"
BASE COUNT          347 a          215 c          230 g          309 t
ORIGIN      
        1 atttagaaat tccgtttggg cataacggag gttggattac cgttggtaag cagaaagaag
       61 gagttggaca tgaatatgtt gcaccaggtt cgcagctaag ttttactgag cggggtactg
      121 cagttacata atttgtaaag caacgcaaca ttggatttcg ttatagtaat agcatattaa
      181 atcatcgtct ggcttttaca ataggggcgt ttaataacgc cctcgaaaca gctaacacca
      241 attcgtttag cgctaatgga tatcaattta caaccagggc tactggtttg gttcattata
      301 aaagcgatcg gtcattcgta catgttggtg ccggatggcg atatgccgat gcccctaaaa
      361 acaagttgag ttataaagca aagcccgaaa ccaatacggc cccttacttt ttaaacacca
      421 atagttttga tgctcaaaat gcacaagcat acatgtttga actaattgga acaaaaggcc
      481 ctttgtcatt aatttctgaa tatatgttac aaatagctca tacacctgaa tcaaaacaaa
      541 gtttaattat tggcaatgtg ctgttagttg gtttattaca ggcgaaaacc ggaaatacaa
      601 taagcagaac ggcaatgttg gcaaattaat tcccaataaa aattttactt ttcgtaacgg
      661 tggtggcctt ggcgctattg aattagctac acgcataacg gtatcagatt ttaacatggc
      721 acaaatagca ggcggtactt ttacccggtt tacagcagcg ttaaactggt ttccaaatgc
      781 acacttaagg tgaagcgcca tttatggata tggaactttg gatttaatgc aacaaacggg
      841 ctataccaat cactatcaat ttaggctgca gtttgagttg taacagaaat tgtaataaag
      901 aattttcaat tagcaactgc tataagtgaa tggccccact gccacaggca gccaaacaag
      961 cttctttaaa cgactccgta taggtggggt gtgcatgact catgcgtgtt atatcttcgg
     1021 cactggccct aaactccata gcaactacgg cttcggcaat taaatcggca caacgtgggc
     1081 caatcatgtg tacgcctaaa a
//
LOCUS       DAOMAA010000946         1100 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_180820_length_1100_cov_2.672727, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000946 DAOMAA010000000
VERSION     DAOMAA010000946.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_180820_length_1100_cov_2.672727"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(222..458)
                     /locus_tag="PLO59_10960"
     CDS             complement(222..458)
                     /locus_tag="PLO59_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01673.1"
                     /translation="MKNSIKFIAVAFALATAVACSSNKVAEGTEAVDSAAAAATEAAV
                     TTIDSAAAAATAVVDSAAAAATTVVDSAAAAVTK"
     gene            712..>1100
                     /locus_tag="PLO59_10965"
     CDS             712..>1100
                     /locus_tag="PLO59_10965"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HQV01674.1"
                     /translation="MDTYTDNDFIEGFRQNNQSLLNVFYKMHFASIQQLIVLNGGDAQ
                     EAKDIYQEGIIVIYEKILDNKLVLTSSLKTYLYSICRNLWLKQLSRKSKYVGNLNDSD
                     DFFAIEPDDAQWMEEEIKQFDGMKQAL"
BASE COUNT          377 a          181 c          197 g          345 t
ORIGIN      
        1 ttaaatgtgt agcctgtttg aatggcttgc gtaaaatttg ccaaattgtt ttccatactc
       61 gttttgctat aagctattta taatgatttt gtttttgatg aaacggcaaa ttaattgatt
      121 taatgcaagc aaaaatgcca caacggtttt attccgttgt ggcatataaa acaaaacccc
      181 gatagataac tatcggggtt tgtaaatgct ttatactaaa attacttagt aacagcagca
      241 gcagctgaat caactacagt tgtagcagca gcagcagcag aatcaacaac agcagttgca
      301 gcagcagcag ccgagtcaat tgtagttaca gcagcttcag ttgcagcagc agcagctgaa
      361 tcaacagctt cagtgccttc ggcaactttg tttgatgaac aagcaacagc agtagctaat
      421 gcgaaagcta cagcgataaa tttgattgaa tttttcattt tgttttgttg aattatttaa
      481 cgttaaactt gctcttaata cggcacacat taaaaggtaa cccatccaga ttaaaaaaaa
      541 cagatatttt tttaatttat attgattatc aataacatac agtttaattt attttcagaa
      601 acatatacgc accattacat atatgcaatt accggcaagc aaaaacctat tttatcaata
      661 caagtgggtt acctttatta aattaatgta ttaaccattg caaaaccatt tgtggatact
      721 tacaccgaca acgactttat agaaggattt aggcaaaaca atcaaagctt gcttaacgtg
      781 ttttacaaaa tgcattttgc aagcatacag cagctcattg ttttaaatgg cggtgatgcc
      841 caggaagcaa aagatattta ccaggaaggc attattgtta tttatgaaaa aatattagac
      901 aataagttgg tgcttaccag ttctttaaaa acgtatttgt attcgatttg ccgtaactta
      961 tggcttaagc aattaagccg taagagtaag tatgttggta acctgaacga tagtgatgat
     1021 ttttttgcca ttgaacctga tgatgcgcaa tggatggaag aagagataaa acaatttgat
     1081 ggcatgaaac aagctttatt
//
LOCUS       DAOMAA010000947         1100 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_180821_length_1100_cov_2.666986, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000947 DAOMAA010000000
VERSION     DAOMAA010000947.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_180821_length_1100_cov_2.666986"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1039
                     /locus_tag="PLO59_10970"
     CDS             <1..1039
                     /locus_tag="PLO59_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HQV01675.1"
                     /translation="SPGKHKGYAYARGKNPTRTALENCLAALEDSKHALCFSSGMGAI
                     DAIVKLLKPGDEVIAGNDLYGGTYRMFTKVFAQYQIVFHFIDLSDEQTIAKCINSNTK
                     LLWVETPTNPTMQIVDLEMCSRIAQKHGLIFAVDNTFASPYLQNPLKWGAHIVMHSVT
                     KYLGGHSDVIMGALCLNDDDLYQKLAFIHNSCGATPGPMDSFLVLRGLKTLHLRMERH
                     CTNGRKVAEFLKSHPRIEKVYWPGFESHPNHHIAQKQMRDFGGMISFVLKNASLEQTF
                     KIASSFNVWTLAESLGGVESLVNHPATMTHASIPKAEREQAGVVDNLLRLSTGVEHID
                     DLIEDLKQALA"
BASE COUNT          334 a          218 c          221 g          327 t
ORIGIN      
        1 aagtccggga aaacataaag gatatgctta tgcacgcggc aaaaacccta cacgtaccgc
       61 tttagaaaac tgcctggcag cacttgaaga ttccaaacat gcactttgtt tttcaagcgg
      121 tatgggcgcc attgatgcca ttgtaaaact attaaagcca ggcgatgaag taattgccgg
      181 taacgattta tatggtggca cataccgaat gtttaccaag gtttttgctc aatatcaaat
      241 tgtttttcat tttattgatt taagcgatga gcaaaccatt gctaagtgca taaacagtaa
      301 taccaaattg ctttgggtgg aaacacctac caaccctacc atgcaaatag ttgatttgga
      361 aatgtgcagc cgtattgcac aaaagcatgg gttaattttt gctgttgaca atacttttgc
      421 atcgccatac cttcaaaacc cattaaaatg gggtgcacac atagtaatgc acagtgttac
      481 taaatatctg ggaggccata gcgatgtaat aatgggcgca ttgtgtttaa atgatgatga
      541 cttataccaa aaattagcat tcattcataa ttcttgtggt gcaaccccag gcccaatgga
      601 tagcttttta gttttacgcg gcttaaaaac tttacacctt cgtatggaaa ggcattgtac
      661 taacggccgc aaagtggccg agtttcttaa aagtcacccg cgtatcgaaa aagtttattg
      721 gccgggtttc gaatcgcatc ccaatcatca cattgctcaa aaacaaatgc gcgactttgg
      781 tggtatgatt tcatttgttt taaaaaatgc cagcttagaa caaacattta aaatagcctc
      841 ctcattcaat gtttggactt tggccgaatc gttgggtggt gttgagtcgc ttgtaaatca
      901 tccggctact atgacgcatg cctctatacc caaggccgaa cgcgaacaag caggcgttgt
      961 agataatttg ttgcgtttaa gcaccggagt tgaacacatt gatgatttga ttgaagattt
     1021 gaaacaagca ctcgcataat tgtctcaacc ttgtaaaagc cttcattata aggcttttaa
     1081 ctacattttt atatagattt
//
LOCUS       DAOMAA010000948         1098 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_181469_length_1098_cov_2.711409, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000948 DAOMAA010000000
VERSION     DAOMAA010000948.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_181469_length_1098_cov_2.711409"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="PLO59_10975"
     CDS             complement(<1..259)
                     /locus_tag="PLO59_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01676.1"
                     /translation="MFHFISEFDIKQMQYFSIIVMVIQQKHIRISIACFLTGIILFNA
                     LAATICYPLALFKHYKDVRSKMPEKIVQFSLGVAAFKKAKVN"
     gene            complement(312..>1098)
                     /locus_tag="PLO59_10980"
     CDS             complement(312..>1098)
                     /locus_tag="PLO59_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016148310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV01677.1"
                     /translation="VVGNTVAIGEKLGKVGSSGNSTGPHLHFEVYNASNTLIDPYQGA
                     CNTLNNNSWWKNQLPYYDSHLNHIATHSTPPVYPSCPTIETTNEATSFVRGNVVYFAS
                     YFHDQVSGSVASYRITRPNGTTYSSWNNTFSVHYAASYWYWYFTIASNEAVGTWQYRC
                     IYNGDTIVRNFNITATARTVADEPDANTWLHIYPNIVTDHLHFDYSIETNTEAHIIDA
                     TGRLIKTTTLNAMFTETDINVSTIKPGVYMLIVANEKQRFIKQ"
BASE COUNT          324 a          205 c          217 g          352 t
ORIGIN      
        1 cattaacttt tgccttttta aatgctgcaa cacctaagct aaattgcaca atcttttcag
       61 gcattttact gcgtacgtct ttatagtgtt taaacaatgc caatgggtaa caaatggtag
      121 cagccaaagc attaaacaat ataatgcctg ttaaaaaaca ggctatactt atgcgtatat
      181 gtttttgctg tatcaccatc acaatgatac taaagtattg catttgtttt atatcaaact
      241 cagaaatgaa atggaacatc tccaattcat tcggtttgtt tcttcagcag taaacaatat
      301 ggtattattg tttactgctt tataaaacgc tgtttttcgt tagctactat aagcatataa
      361 acacccggct taattgtaga aacgtttata tcagtttcgg taaacattgc attaagcgta
      421 gttgttttaa tcaatcggcc tgttgcatca ataatgtgag cttcggtgtt tgtttctata
      481 ctataatcaa aatgtaaatg gtcggtaaca atatttggat aaatgtgtaa ccatgtgttt
      541 gcatccggtt catcggccac ggtgcgtgct gtggctgtaa tattaaaatt acgtacaatg
      601 gtatcgccat tgtaaataca tctgtattgc caggtaccta ccgcttcgtt tgacgcaatg
      661 gtaaaatacc agtaccaata agatgctgca taatgaacac taaatgtatt attccaactg
      721 ctataggtgg taccattggg tcgtgttata cgataactgg caacgctgcc actaacctgg
      781 tcgtgaaaat agctggcaaa ataaaccacg ttgccacgta caaatgatgt ggcctcattg
      841 gtggtttcga tggtagggca tgacggatat accggagggg tgctatgcgt agcaatgtga
      901 tttaaatgcg aatcgtaata gggtagttga tttttccacc aagagttatt gtttaaggta
      961 ttacaagctc cctgataagg atcaataagt gtattggatg cattgtacac ttcaaaatgc
     1021 aggtgcgggc cagtagaatt accgctactg cctaccttac caagcttttc gccaatggcc
     1081 actgtgttgc ctaccact
//
LOCUS       DAOMAA010000949         1098 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_181632_length_1098_cov_1.780441, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000949 DAOMAA010000000
VERSION     DAOMAA010000949.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_181632_length_1098_cov_1.780441"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="PLO59_10985"
     CDS             <1..563
                     /locus_tag="PLO59_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01678.1"
                     /translation="AKNTAYNNAVNARIATFDGFRALCTRLINAFQTTDATSEKIADA
                     KGFNRKIQGQRSTKTATPVDASTPAPTTISSSHQSYDQLIQHFAGLISVLQSEPTYAP
                     NENDLKIATLTAKQTDMTAKNTALATAFTAISNSRIARDKVLYKDITGLVPIANDVKK
                     YIKSIFGASSAEYAEVKSIEFKNVKK"
BASE COUNT          391 a          245 c          175 g          287 t
ORIGIN      
        1 tcgccaaaaa tacagcttac aacaacgcag taaatgcacg catcgcaact tttgatggct
       61 ttcgtgcatt atgcacacgc ttaataaatg cttttcaaac aacggatgcc acttctgaaa
      121 aaattgcaga cgcaaaaggg ttcaaccgca aaattcaagg gcaacgttcc accaaaacag
      181 caacaccagt tgatgcaagt acaccagcac caaccactat aagttcatca caccaatctt
      241 atgatcaatt gatacaacac tttgctggtt taatttctgt tctacaatca gaaccaacct
      301 atgcaccaaa cgaaaacgac ttgaaaattg cgacacttac agcaaaacag acagacatga
      361 cagcgaaaaa tacagcatta gcaactgcat ttacagccat cagtaattca cgaattgcgc
      421 gcgacaaagt tttgtataaa gacataacag gacttgttcc aatcgccaac gatgttaaaa
      481 aatacatcaa atctatcttt ggcgcaagta gtgccgaata cgcagaggta aaaagcattg
      541 aatttaagaa cgttaagaaa taaattaacc gcttttaata ttatagctac cactttttaa
      601 aatatcgcta ctgcgttaac aaatagcgtt actgcgttaa caattaacat tactgcggtt
      661 tgaataattt ctactgcgac tataaatatc tttactgcgt taacaaatat cgttactgcg
      721 acttatcttt tagctactgc gttaacaatt aaagcaactg cggtttatat tagagcaact
      781 gcggaaataa tactccttac tgcgaaaaac caagctgttg catatacatt tgcattttag
      841 ccaacgccaa agcaatgctt aaaatgcaaa agcctatgca accctaccct attcggttcg
      901 gctcgctcga ccgatagaca atgcaaacct aatcgaaaaa gggctaccta caacatggta
      961 tttgcaataa ggcaggtaga tttggtaact taacgcaata tgtataaatt tacagtaagc
     1021 aaggtattta cagtttgtag cactcattct gcctcatcgc aaatacccag ccgttagctg
     1081 acattataaa accaacgt
//
LOCUS       DAOMAA010000950         1096 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_182131_length_1096_cov_2.518732, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000950 DAOMAA010000000
VERSION     DAOMAA010000950.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_182131_length_1096_cov_2.518732"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="PLO59_10990"
     CDS             <1..302
                     /locus_tag="PLO59_10990"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HQV01679.1"
                     /translation="TNSNQKVITQTNYSGGIQGGISNGMPIYCKVAFKPVASINQLQQ
                     TVNNKSETVSIKVEGRHDPCVVPRAVPVVEAMFALVLINAHLANNQAKWPNLKSI"
     gene            299..>1096
                     /locus_tag="PLO59_10995"
     CDS             299..>1096
                     /locus_tag="PLO59_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008510404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HQV01680.1"
                     /translation="MNVEIVTIGDELLIGQVVDTNSAWMGDTLNKAGFAVKQITSVSD
                     NEGRIIEALEKAKQNADIILITGGLGPTKDDITKVSLCKFFNTHLVFSDEAYAQVSHF
                     FTLRGSDVSDVNKLQAMLPANCHVLHNKVGTAPGMWFNTDGKIFVSMPGVPYEMKLLM
                     TDEVLPRLKNIFATPAILHKTILTQGIGESVIAEKIADIEDALPPYIKLAYLPAVASV
                     RLRLTGISNNTDLENELNTLVADIEARIKPYVYGYDNDTLPAVVGRLL"
BASE COUNT          359 a          208 c          225 g          304 t
ORIGIN      
        1 ctaccaatag caatcaaaaa gtaatcacac aaaccaatta ttcaggtggc atacaaggtg
       61 gtattagtaa tggcatgccc atttattgta aagtggcatt taagcctgtg gcatcaataa
      121 atcaactaca acaaaccgta aataataaat cagaaacagt aagcattaag gtagaaggcc
      181 ggcacgaccc ctgtgtagta ccgcgtgcag taccggtagt tgaagccatg tttgccttag
      241 ttcttataaa tgcccactta gccaacaacc aggcaaagtg gccaaactta aaatcaatat
      301 gaatgtagaa attgttacca taggcgatga attattaatt gggcaagtag ttgatacaaa
      361 ctcggcctgg atgggcgata ccttaaataa ggccggcttt gctgtaaagc aaattacatc
      421 ggttagtgat aacgaaggtc ggattattga agccttagaa aaagcaaaac aaaatgccga
      481 tatcatttta ataacgggcg gtttaggtcc taccaaagac gatattacca aagtatcact
      541 gtgcaaattt tttaataccc atttggtttt tagtgatgag gcttatgcac aggttagtca
      601 tttttttacc ttgcgtggaa gcgatgtaag cgatgtaaat aaactacaag ctatgctacc
      661 ggctaattgc catgttttac ataataaagt tggtacagca ccgggtatgt ggtttaatac
      721 ggacggtaaa atatttgttt cgatgcccgg tgtaccctat gaaatgaaac tgctaatgac
      781 ggatgaagtg ttgccccgac ttaaaaatat ttttgctaca cctgccattt tacataaaac
      841 cattttaacg caaggcattg gcgagtcggt aatagccgaa aaaattgccg atatagaaga
      901 tgccttacca ccctatataa aattagcata tctgcctgcc gttgccagtg tgcgtttacg
      961 cttaacaggt ataagcaata atactgattt agaaaatgaa ctgaacacct tagttgccga
     1021 tatcgaagcc cgcataaagc cttacgttta tggatacgat aacgatactt tgcctgctgt
     1081 agttggcaga ctgtta
//
LOCUS       DAOMAA010000951         1096 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_182175_length_1096_cov_2.247839, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000951 DAOMAA010000000
VERSION     DAOMAA010000951.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_182175_length_1096_cov_2.247839"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(217..864)
                     /locus_tag="PLO59_11000"
     CDS             complement(217..864)
                     /locus_tag="PLO59_11000"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQV01681.1"
                     /translation="MQPIQVLIFDDHAEMRKMYALMLSTETDMICIGAYSNCMDAVKQ
                     VDKHKPDVILMDIEMPGKSGIDAVKEIKTAYPDMKIIMQTIFSDDDKIFNSICNGASG
                     YILKNSPLSDFTNAIRQVCNGGAPMSPGIALRVIERFRKEVKPVNLPETQFNLTDKEL
                     EILNLLVSGLSYKLIADKINKSYHTVDSHIRKIYEKLHVRSATEAVRKAIDHRIL"
     gene            complement(911..>1096)
                     /locus_tag="PLO59_11005"
     CDS             complement(911..>1096)
                     /locus_tag="PLO59_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HQV01682.1"
                     /translation="TQLIFIIEDDGIGFDASLETTGNGIQNMHIRAQQMQSFLNIESH
                     SGSGTKIYLKVPFTNIR"
BASE COUNT          326 a          179 c          203 g          388 t
ORIGIN      
        1 atacttatgc ctgtaataag cattagtgtg taaatggttt ttagtaaaaa attttttctc
       61 atgattgaat gtttttaagg tatggtattt atattatatt gtaacagggt gattaaacaa
      121 acatataaag catttggatg tatttgagcc aatttaagtt taagttggta aaaatagagt
      181 cagtttattt tattacaatc acgaaattac gtgtaattat aaaattcggt ggtcgatagc
      241 tttgcgcact gcttcggttg ctgagcgcac atgtaatttt tcatatattt ttcggatatg
      301 cgaatcaaca gtatggtagc tcttattaat tttatcggca attagtttat aactaagacc
      361 tgaaacgagc agatttaaaa tttctaattc cttatcagtt aaattaaatt gggtttcggg
      421 taagtttaca ggcttcactt cttttctgaa ccgttctatt acacgtaatg caatgcccgg
      481 gctcataggc gctccaccat tacaaacctg cctgatggca ttggtaaagt cggataaggg
      541 gctgtttttt aaaatatagc cagaggcgcc attacatatc gaattaaaaa ttttatcgtc
      601 atcactaaaa atggtttgca taataatttt catatcgggg tatgcagttt taatttcttt
      661 aacggcatca atgcccgatt tacccggcat ttcaatatcc attaaaataa catcgggctt
      721 gtgtttatca acttgcttta cggcatccat gcaatttgaa tacgcgccta tgcaaatcat
      781 gtccgtttca gttgacaaca ttaatgcata catttttcgc atttcggcat ggtcgtcaaa
      841 aatcaatact tgaatcggtt gcatggtagt atattatagt gcaaacttaa ctttggctta
      901 ctaaagtgca ttaacgaata ttcgtgaaag gaacttttaa atagattttg gttccgctac
      961 ctgaatgcga ttcaatgttt aaaaagcttt gcatttgttg cgcacggata tgcatgtttt
     1021 gaatgccgtt gccggtggtt tcgaggctgg cgtcaaaacc aatgccatca tcttctatta
     1081 taaaaataag ttgtgt
//
LOCUS       DAOMAA010000952         1094 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_182752_length_1094_cov_2.619827, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000952 DAOMAA010000000
VERSION     DAOMAA010000952.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_182752_length_1094_cov_2.619827"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..>1094)
                     /locus_tag="PLO59_11010"
     CDS             complement(10..>1094)
                     /locus_tag="PLO59_11010"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HQV01683.1"
                     /translation="NASELLMQCLTFAGLVFIWRFIILRGIKFLRMQGLNYKRVIILG
                     GGHQAVDMYGFFNNKARYGYKLLGAFTLKPFDDMPVDFKESSLEAVEKFCIENEIDEI
                     FCSLSGLRNDDVAEIFRFADKNLIRFKVIPDFKGFFNKKVIVDFYDMIPVLVMRPEPL
                     ESLSNRVIKRLFDIVFSLGVIICVFPWLFPVFAIIIKATSEGPVFFKQKRSGKNNEEF
                     TCLKFRTMRVNSEADEKQATRTDPRITPIGAFLRKTSLDELPQFFNTLWGDMSVVGPR
                     PHMLRHTQEYSEMIDKFMVRHFAKPGITGWAQAKGFRGDTVDPVLMQKRVELDVWYVE
                     NYSFLLDIKIILLTVWVIFRGQKEAF"
BASE COUNT          355 a          233 c          194 g          312 t
ORIGIN      
        1 tatcgtttat taaaaagctt ctttctgacc tctgaaaata acccaaaccg ttagcagaat
       61 aattttaatg tctaataaaa agctataatt ttcaacgtac caaacatcta actcaacacg
      121 cttttgcatt aacacagggt caacggtatc gcctctgaaa cctttagctt gtgcccaacc
      181 ggttatgccg ggttttgcaa aatgacgtac cataaatttg tcaatcattt ccgaatactc
      241 ttgggtgtgg cgcagcatgt gtggcctggg accaactaca ctcatatcgc cccaaagggt
      301 attaaaaaac tgcggtaatt catccaaact ggttttacgc aaaaaggcgc caatgggtgt
      361 aatacgtggg tcggtgcggg tggcttgctt ttcgtctgct tcggagttta cacgcatggt
      421 acgaaacttt aaacacgtaa actcttcatt gtttttgccc gagcgttttt gtttgaaaaa
      481 tacagggcct tcgctggttg cttttattat gattgcaaat accggaaaca accaggggaa
      541 aacacaaata attacaccta aagaaaaaac aatgtcaaac aaccgtttta ttacgcggtt
      601 tgataacgac tctaatggtt cggggcgcat taccaaaacg ggtatcatat cataaaaatc
      661 aacaattacc tttttattaa aaaagccctt aaagtcgggt attactttaa atcgtatcag
      721 gtttttatcg gcaaacctga aaatttcggc tacatcatca ttacgtaacc ctgataaaga
      781 acaaaagatt tcatctatct cattttcgat gcaaaatttt tctacagcct ctaaagatga
      841 ttctttaaaa tcaacgggca tatcgtcaaa tggtttaagc gtaaaagcgc ctaaaagttt
      901 gtacccataa cgtgctttgt tattaaagaa gccatacata tcaacggctt gatgcccgcc
      961 acctaaaata attacccgct tataatttaa gccttgcatg cgcaaaaact taatgccacg
     1021 tagtatgata aaccgccaaa taaaaaccag cccggcaaaa gtgaggcatt gcattagcaa
     1081 ctcagaggca ttaa
//
LOCUS       DAOMAA010000953         1093 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_183041_length_1093_cov_3.046243, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000953 DAOMAA010000000
VERSION     DAOMAA010000953.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_183041_length_1093_cov_3.046243"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..177)
                     /locus_tag="PLO59_11015"
     CDS             complement(<1..177)
                     /locus_tag="PLO59_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQV01684.1"
                     /translation="MYALQMQNITKSFGNVQALKNISFSVAPAEIHALCGENGAGKST
                     LMKIIYGEHRSDPGG"
     gene            complement(188..>1093)
                     /locus_tag="PLO59_11020"
     CDS             complement(188..>1093)
                     /locus_tag="PLO59_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HQV01685.1"
                     /translation="TYTEERWKKDGQFFEERAAQLGATVIRQVCNGNEQLQNEQVENL
                     ITQGVDVLVIVPHNSKTAAKAVNSAKEAGIKVIAYDRMIKDCDLDLYVSFDNEQVGVL
                     QAQTLVGLAPKGNYMLVEGSATDENAFLYNKGHMQILKPLIDSGHITIVMEQYCKDWL
                     PIEAYKQTQNALTKNNNNIAAILVANDGLAGGCIQALTEQQLAGKIPVSGQDAELAAV
                     QRVIDGVQSMTVYKPIKQLAYAGAEAAIALAENKPTAATTRTINNGKKEVSALLLAPV
                     AVDKNNVKQTVIADGYIKATELKMQ"
BASE COUNT          270 a          229 c          200 g          394 t
ORIGIN      
        1 tcctccgggg tctgaacgat gctctccata gatgattttc atcaatgtcg atttgccggc
       61 accgttttcg ccacaaaggg catgtatctc ggccggagca acactaaagc tgatattttt
      121 taatgcctga acattgccaa aacttttggt aatgttttgc atttgtaatg catacataat
      181 tgtagggtta ttgcattttt aattcagttg ctttaatgta accatcggca ataaccgttt
      241 gcttcacatt gtttttatct actgctaccg gtgcaagtag tagtgcacta acttcttttt
      301 taccattgtt aatggtgcgt gttgtggctg ctgttggttt attttcggct aaagctattg
      361 ctgcttcggc accggcatag gctaattgtt ttataggttt gtaaaccgtc atactttgaa
      421 caccatcaat aactctttgt acggcagcaa gctcggcatc ttgtccgcta acgggaattt
      481 ttccggccaa ttgttgttct gttaatgctt gtatgcagcc tccggctaac ccatcgttgg
      541 caactaaaat ggcagcaatg ttattgttgt ttttggtcaa tgcattttgg gtttgtttat
      601 aagcttcaat aggcaaccaa tctttgcaat attgctccat cacaatggtt atgtgtccac
      661 tatcaattaa aggtttaagt atttgcatgt ggcctttatt atataaaaaa gcattttcat
      721 cagttgccga accttctacc aacatataat tgcctttagg ggctaagcca accaaagttt
      781 gtgcttgtaa aacacccact tgttcattgt cgaatgaaac gtataaatcc aaatcacaat
      841 ctttaatcat cctatcgtat gcaattactt ttatacctgc ttcttttgcg ctgtttacag
      901 cttttgcagc ggttttacta ttgtgcggca ctattactaa aacatcaact ccttgtgtaa
      961 ttaaattttc aacttgttcg ttttgtaatt gttcattacc gttacaaact tgccttatta
     1021 cggtagcacc taactgagcg gcacgctctt caaaaaactg cccgtctttt ttccaacgtt
     1081 cttcggtgta ggt
//
LOCUS       DAOMAA010000954         1092 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_183333_length_1092_cov_3.675024, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000954 DAOMAA010000000
VERSION     DAOMAA010000954.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_183333_length_1092_cov_3.675024"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="PLO59_11025"
     CDS             complement(<1..767)
                     /locus_tag="PLO59_11025"
                     /inference="COORDINATES: protein motif:HMM:NF013636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proprotein convertase P-domain-containing
                     protein"
                     /protein_id="HQV01686.1"
                     /translation="MIKKVLSFCFFYFHLTAQAQTFNGTGGAIPDNGNSVDFVIPVNG
                     LPSVIDSGFGVETVCINLTHSYDADLDIALIAPDGTQIILAASVGGSGNNFTNTCFNQ
                     NATAPITQGNAPFTGTFKPMGILGNVNNTQNPNGNWTLHILDTYPFADDGDLIDWNIT
                     FGNNPAYANPFKQSHLPIVIINTNGQTIPNDPKLTAQMGIIYNGPGIMNQLTDTPNTY
                     NGFIGIEVRGSSSAGFPQKQYAVETRDSLGNNLDVSL"
     gene            874..>1092
                     /locus_tag="PLO59_11030"
     CDS             874..>1092
                     /locus_tag="PLO59_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01687.1"
                     /translation="MLQPAFIEILLLSHSTIFNKMITRPLTITYPPYYSKYIQLVPET
                     SPTETLLHQMQAVIQLFNSLTQTQLLFKY"
BASE COUNT          336 a          206 c          205 g          345 t
ORIGIN      
        1 aataaactca catctaaatt attacctaaa ctatcgcgtg tttcaacagc gtattgcttt
       61 tgcggaaagc ctgccgaact gctgcctcgt acttcaattc caataaaacc attgtacgta
      121 ttgggtgtat cggtaagctg attcatgatg ccggggccat tgtaaataat gcccatttgt
      181 gcagttagtt tggggtcgtt aggtatggtt tgtccgttgg tgtttataat aacaataggc
      241 aaatgcgatt gtttaaacgg gttggcataa gccgggttgt tgccaaaggt tatattccaa
      301 tcaatcaaat caccatcatc ggcaaaggga taggtgtcta atatatgtaa tgtccaattg
      361 ccattcgggt tttgcgtgtt gtttacatta cccaatatac ccataggttt aaaagtgcct
      421 gtaaatggtg cattgccttg cgttatgggt gccgtagcat tttgattaaa acaagtattc
      481 gtaaaattat tgccgctgcc acctacagag gctgccaaaa taatttgtgt gccatcgggt
      541 gcaattaatg ctatatccaa atcggcatcg taagaatgtg tgaggttgat gcaaaccgtt
      601 tctacgccaa accctgagtc aattacactg ggtaaaccat taaccggaat tacaaaatca
      661 acactgttac cattatcggg tatggcacca ccggtaccgt taaatgtttg tgcttgtgca
      721 gttaaatgga aatagaaaaa gcaaaagctc agtacttttt taatcatagt ttaaatagtg
      781 taaatctgtt gaacaggcac aaagctaaat tataattgat agtttattta actgaaagca
      841 gtaagacttg cctgttgttt taaaattgca ttcatgttac aacctgcttt tattgaaatt
      901 ttattactta gccattcaac tatttttaat aaaatgataa cacgtccatt aactattacc
      961 tatccaccct attacagcaa atacattcaa ttggtgcccg aaacatcgcc aacagaaaca
     1021 ttgttgcatc agatgcaagc ggttattcaa ctgtttaaca gcttaacgca aacacaatta
     1081 ctatttaagt at
//
LOCUS       DAOMAA010000955         1092 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_183484_length_1092_cov_2.146577, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000955 DAOMAA010000000
VERSION     DAOMAA010000955.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_183484_length_1092_cov_2.146577"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="PLO59_11035"
     CDS             <1..737
                     /locus_tag="PLO59_11035"
                     /inference="COORDINATES: protein motif:HMM:NF020932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HQV01688.1"
                     /translation="DSWPVNIVDLENVLNSGNAITPAFLQAQDGTLKGFHTIEFLLWG
                     SSSNKQVTDFTAREFEYLAAAATVLASDAKTLYDMWQPASGNFIAQVQHAGHGSNIYI
                     SQKAAIEEMTSALVIIADEVGNGKINEPFSQSDLSLEESRFSANSKTDFADNMRSIQN
                     IYTGTYGAMPAGNGIGAIIQSKNNALHQQVLTHINNAITSIEAIPGTFSDAVINNRPA
                     IENAQKAVRDLQKLLEEQVTPLISNL"
     gene            739..>1092
                     /locus_tag="PLO59_11040"
     CDS             739..>1092
                     /locus_tag="PLO59_11040"
                     /inference="COORDINATES: protein motif:HMM:NF018269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HQV01689.1"
                     /translation="MYRLTVVALIIIFFFGSCTKEESKDVTVFEAGGKTTIYSSGPDA
                     YTFPLANASASTIDAHFKADADFAQQFVSAPALFGGLGPVFNQNACESCHIRNGRSAV
                     PQFNGDMQSGLLLKIS"
BASE COUNT          351 a          202 c          231 g          308 t
ORIGIN      
        1 tggattcatg gcctgtaaat attgttgatt tagaaaatgt acttaacagt ggtaatgcta
       61 taacacctgc gtttttgcaa gcacaagacg gaacattgaa gggttttcac accattgaat
      121 ttttattgtg gggcagtagc agcaataagc aagttactga ttttacagcg cgcgagtttg
      181 aatatctggc agccgcagca actgtattag ccagcgatgc aaaaacgctt tatgatatgt
      241 ggcaaccggc atcgggtaat tttattgcac aagtacagca tgcaggccat ggcagcaata
      301 tttatattag tcaaaaggcc gctatcgaag agatgacaag tgccttagtt attattgctg
      361 atgaagtagg aaatggcaaa ataaatgaac ccttctcgca aagcgattta tcgctggaag
      421 aatcacgctt cagcgccaat tcaaaaaccg attttgcgga taacatgcgc agcatacaaa
      481 acatctatac cggtacgtat ggtgcaatgc ctgcaggtaa tggaattgga gccataattc
      541 aatcaaaaaa caatgcactg catcagcaag tattaacgca tattaataat gcaataacat
      601 ctatagaagc aataccaggc actttttctg atgctgtaat taataacaga ccggcaatcg
      661 aaaatgcaca aaaggcagtt cgcgatttac aaaagttatt agaagaacag gtaacaccac
      721 ttatcagtaa tttataatat gtataggctg acggttgttg cacttatcat tattttcttc
      781 tttggtagtt gtaccaaaga agaaagtaaa gatgttacag tttttgaagc aggtggaaaa
      841 actaccattt atagtagtgg ccccgatgct tatacatttc cattagccaa tgcaagtgca
      901 tcaaccattg atgcgcattt taaagccgat gccgattttg cacagcaatt tgtttcagca
      961 cctgcattgt ttggtggctt gggacctgtg tttaatcaaa atgcctgcga aagttgccat
     1021 atcagaaacg ggcgttcggc agtaccacaa tttaatggcg atatgcaaag tggtttgctg
     1081 ttaaaaattt cg
//
LOCUS       DAOMAA010000956         1090 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_184004_length_1090_cov_3.100483, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000956 DAOMAA010000000
VERSION     DAOMAA010000956.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_184004_length_1090_cov_3.100483"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="PLO59_11045"
     CDS             <1..876
                     /locus_tag="PLO59_11045"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQV01690.1"
                     /translation="LIIPSYNRAGFIAKAIQSATQQTFSSIQIIVIDDGSTDNTNDVV
                     ASLNLPNLQYYKTENAERAAARNFGVSKATGQYITFLDSDDYLLPNHFEVAYRFIQAQ
                     NNPAIFYQPNNFVDANGKVTHPHKKIKDDVTRALIVEGNLMSCIGVFLRNEIAQTFKF
                     NTDRALSGTEDHELWLRIAAHHTILYNRVVTSCMVQHDSRSVMAVNDATLIKRLQLFV
                     YYMKQNQQFIARYKNLLPAMEAQAWSYLSLHFALTGKHKKQAIHFFTKAVRTYPPFIF
                     QKRCAAIIKQLLLNN"
BASE COUNT          368 a          213 c          182 g          327 t
ORIGIN      
        1 ttaataattc ccagttataa ccgcgctggt tttattgcca aggcaattca atcggctacg
       61 cagcaaacat tcagttctat tcagattata gtaattgatg atggcagcac cgacaacaca
      121 aatgatgtag ttgcaagttt aaatttacct aatctgcaat actataaaac cgaaaatgcc
      181 gaacgtgcag cagcccgaaa ttttggcgta tcaaaagcaa cagggcaata cattactttt
      241 ttagactcgg acgactactt acttccaaac cattttgaag ttgcttaccg gtttatacaa
      301 gcacaaaata accccgccat tttttaccaa cccaataatt ttgtggatgc caacggaaaa
      361 gttacccatc cgcataaaaa aattaaggac gatgttaccc gtgcattaat tgtagaaggc
      421 aacctgatga gctgcatagg cgtgttttta agaaatgaaa tagcacaaac atttaaattt
      481 aataccgaca gggctttaag cggtaccgaa gaccatgaac tttggctacg tattgctgca
      541 catcacacca tactttataa tcgcgtagtt acctcgtgca tggtacaaca cgatagcaga
      601 agtgtaatgg ctgtaaatga tgcaacactg atcaagcgat tgcagttatt tgtgtattat
      661 atgaagcaaa accagcaatt tattgccaga tataaaaatt tactgccagc catggaagcg
      721 caagcctggt cgtacttatc gctacacttt gcattaacag gcaagcataa aaaacaagca
      781 attcattttt tcacaaaagc tgtacgtacc tacccgcctt ttatatttca gaaaagatgt
      841 gcagccataa ttaaacaact gcttttaaac aattagtttt taattgaaaa ttttagcaat
      901 aaaaaaaata ggatatgctt agaatagctt ttaaaaaaat tattgattta taaattttat
      961 tggcaatttc ttaaactaat agggtataaa acatttttat atttgccgcc cttaaaaatc
     1021 tcccttgtat gcttgcaaac attgacttta gttttgcgcc tcttttaact tcggccggct
     1081 tggtaagttt
//
LOCUS       DAOMAA010000957         1090 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_184023_length_1090_cov_2.818357, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000957 DAOMAA010000000
VERSION     DAOMAA010000957.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_184023_length_1090_cov_2.818357"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1090
                     /locus_tag="PLO59_11050"
     CDS             <1..>1090
                     /locus_tag="PLO59_11050"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HQV01691.1"
                     /translation="QQEVVYIYSNSKQGLQHAVYVFIERCLHVNKFATKPLFEKRKTI
                     TVDSTKITDEPAYTTRSLYYTGTNDAEYITWHGLNGKSADSVKDQWGMWVHTFKNLIP
                     DSVYFDTHPEYYALNGVRRRSQLCLSNEAVLQTVIESLRLMMRKDTASLYWSVSQNDN
                     FEYCTCSQCKTIDSIEGSQSGTMLRFVNKVAAAFPQKIISTLAYQYTRKPPAITKPAK
                     NVNIMFCSIECGRHLPIEDDPQAASFRSDFEGWCKLTDNIMVWDYVVQFSNYVSPFPN
                     IHVLQPNIQYFYEHGVRHIFEQASGSNWSDNMELKSYLIAALLWNPYVDVDSLIERYA
                     TLQYGAGADDMITYMVDMEQQLNQSKAPL"
BASE COUNT          354 a          208 c          222 g          306 t
ORIGIN      
        1 caacaagaag tggtttacat atatagtaat agtaaacaag gcttacaaca tgcagtttat
       61 gtttttatcg aacgctgttt gcatgtaaat aaattcgcaa caaagccttt gttcgaaaaa
      121 cggaaaacca taacagtaga tagtactaaa ataacagatg agccggctta taccacacgc
      181 agtttgtatt atacaggcac taacgatgcc gagtatataa cctggcatgg tttaaatggc
      241 aaatctgccg acagtgttaa agaccaatgg ggtatgtggg tacatacctt taaaaactta
      301 atacccgatt cggtttactt tgatacccac cccgagtatt atgctttaaa tggagtgcgc
      361 agacgctcgc aactttgctt gagtaatgag gctgttttac aaactgtaat tgaaagctta
      421 cggcttatga tgcgaaagga tactgcctca ctttactggt cggtaagtca gaatgataat
      481 tttgaatact gtacctgtag tcaatgcaaa acaattgata gtatcgaagg tagccaaagc
      541 ggtaccatgc tcaggtttgt aaacaaagta gcagcggcat tcccacaaaa aataatttca
      601 acactggcat atcaatatac acgcaagcca cctgcaatta ccaagcctgc taaaaatgta
      661 aatattatgt tttgcagtat tgaatgtggc cggcatttac ccatcgaaga cgatccgcaa
      721 gctgcatcct tcagatcaga ttttgaaggg tggtgcaaat taaccgacaa cataatggta
      781 tgggattatg tggtgcagtt ttcaaattac gtttcgcctt ttcccaatat ccatgtactg
      841 caacctaaca tacaatattt ttatgagcac ggtgtacgcc atattttcga acaggcaagc
      901 ggaagtaact ggagcgataa catggaacta aaaagttatt taatagcggc cttgctgtgg
      961 aatccttatg ttgatgttga ctcattaata gagcgttatg ccacattgca atatggagcc
     1021 ggtgccgatg atatgattac ttatatggtt gacatggaac aacaacttaa tcaaagcaaa
     1081 gcacctttgg
//
LOCUS       DAOMAA010000958         1089 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_184290_length_1089_cov_4.038685, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000958 DAOMAA010000000
VERSION     DAOMAA010000958.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_184290_length_1089_cov_4.038685"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..>1089)
                     /gene="porQ"
                     /locus_tag="PLO59_11055"
     CDS             complement(109..>1089)
                     /gene="porQ"
                     /locus_tag="PLO59_11055"
                     /inference="COORDINATES: protein motif:HMM:NF033711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system protein PorQ"
                     /protein_id="HQV01692.1"
                     /translation="TYDFLNLTPAARIAAFGGVALAVKDHDLNMAYQNPAALNYQMHK
                     QFTFNYNPYFADIKSGQMAYAHAIDSNNMVSAALQFVGYGDFTATDNTGNITGSFNAN
                     DYVLNLSYGRSIDSLFSVGATFKTIYSNYESYTSVGVAVDLAATYFNSNKQLCMTLVA
                     RNAGVQLKTYVSDNNEKLPLEVMFGISKRLDKAPFRLGLHVTHLEKFDLSFTNPNSQT
                     TIDPITGEAVTEKTSFGKKIMLHMIPSIEIILSKNFMIRGSYNFQRRNELAFESRKAM
                     AGLSFGFGFGIKRFTLNYGRSVYNLAGATNHFSVGIDLSKSIKKIPGNTQ"
BASE COUNT          319 a          205 c          206 g          359 t
ORIGIN      
        1 ccatgacgtc cccgtaagtg tattaactat ctattttaaa gaactgctgc aaaagtacca
       61 ctaaaaatgc gttgcacgga attatttttt atttaaaccg gcaattgttt attgcgtgtt
      121 acctggtatt ttttttatgc ttttgcttaa atcaatgcct actgaaaaat ggttggtggc
      181 tccggctaag ttatatacgg aacgaccgta gtttaacgta aagcgtttaa tgccaaaacc
      241 aaatccaaat gataggccgg ccattgcttt gcgcgattca aaagcaagtt catttcttcg
      301 ctgaaaatta taactgcccc tgatcataaa gttttttgat agtatgattt caatactcgg
      361 aatcatgtgc aacataattt ttttgccgaa cgatgttttt tctgttaccg cttcaccggt
      421 tatgggatca attgttgttt gtgaattggg gtttgtaaaa ctgaggtcaa acttttcgag
      481 gtgcgttaca tgcaatccca aacggaatgg cgctttatct aatcgtttgc taatgccaaa
      541 cattacttct aaaggtagtt tttcattatt gtccgatacg tatgttttta actgtactcc
      601 ggcattgcgt gctaccagtg tcatacacag ttgcttattg ctattaaaat aagttgcggc
      661 taaatctact gcaacgccaa ccgatgtata actttcatag tttgaataaa tagttttaaa
      721 ggttgcgccc acactaaata aactatcaat actgcggcca taactaaggt ttagtacata
      781 atcgttggca ttaaaactgc ctgtaatgtt gcctgtatta tccgtagctg taaaatcgcc
      841 atagcctaca aattgtaatg ctgcactaac catattatta ctgtcaatag catgtgcata
      901 agccatttgt cccgatttaa tatcggcaaa gtatgggttg taattaaacg taaactgttt
      961 gtgcatttga taattaagtg cagccgggtt ttgataggcc atattcaaat catggtcttt
     1021 aacagcaagg gctacaccac caaatgctgc aatgcgagcg gcaggcgtaa ggtttaaaaa
     1081 atcataagt
//
LOCUS       DAOMAA010000959         1089 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_184369_length_1089_cov_2.558027, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000959 DAOMAA010000000
VERSION     DAOMAA010000959.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_184369_length_1089_cov_2.558027"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            79..>1089
                     /locus_tag="PLO59_11060"
     CDS             79..>1089
                     /locus_tag="PLO59_11060"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HQV01693.1"
                     /translation="MKKIKLLSSLLLFFGLMHGASGQDWITKMKDPDVNFFEVQKAFD
                     KYYVKKEREIERAMKKARKLRNEGVSEEELEVPGFMQYKRWEWFMKPRVYSNGQRFDP
                     SYTFAEMQRYKNNMSTNGAGNWTLIGPTTTKPIGGGSGRLNFVRFDPNNINNIWVGAP
                     SGGLWKSNDGGATWSTNTDLLPSVIGCTDLAIDPTNTNIMYLATGDGDAGDTYTVGLL
                     KSVDGGNTWQSTGLSFFTANGRQMSKILIDPSNTNNLLVATSAGIFRSIDAGITFANV
                     QAGSFKDLEMKPGTPTTYYASGTEVYRSTNSGVSWSKISSGLPAASAVSRLAIAVTPA
                     NAD"
BASE COUNT          335 a          210 c          247 g          297 t
ORIGIN      
        1 gtcaatttgt aatattggta tgagcgcttg attgcttcaa tgtaacgcat acatttgaaa
       61 aataaacaaa cccactttat gaaaaaaatt aaattactaa gttccctttt attgtttttt
      121 ggtttgatgc atggtgcatc aggtcaggat tggattacaa aaatgaaaga ccctgatgta
      181 aacttctttg aagtgcaaaa ggcttttgat aaatactatg taaaaaagga gcgtgaaata
      241 gaacgtgcta tgaaaaaagc gcgtaagcta cgcaacgaag gtgtaagcga ggaagaatta
      301 gaagtacccg gttttatgca atacaaacgc tgggaatggt ttatgaaacc cagagtttac
      361 agcaatggcc aacgctttga cccatcgtac acttttgccg aaatgcaacg ttacaaaaac
      421 aacatgagta ccaatggtgc cggcaattgg acgcttattg gcccaactac cactaaaccc
      481 attggtggtg gctcggggcg gttaaacttt gtgcgtttcg accctaacaa cattaataac
      541 atttgggtgg gtgcaccttc gggcggatta tggaaaagta atgatggcgg tgccacctgg
      601 agtacaaata cagatttatt gcccagcgtt attggatgta ccgatttggc aattgatcct
      661 accaatacca atattatgta tttagctaca ggcgatggcg atgcgggcga tacatatacc
      721 gttggtttac ttaaatcggt tgatggtggc aacacctggc aaagtacagg cctgtcgttt
      781 tttactgcaa acggacgaca aatgagtaaa atattgattg acccaagcaa taccaacaat
      841 ttattagtgg ctacatcggc cggtattttc agaagtattg atgccggtat taccttcgct
      901 aatgtgcaag ccggttcgtt taaagattta gaaatgaaac cgggtacccc aacaacttac
      961 tatgcttcag gtactgaagt ttaccgaagt accaacagcg gtgtaagctg gtcgaaaata
     1021 agcagtggct tacctgctgc atctgccgtt agcaggttag caatagcagt tacgcctgcc
     1081 aatgccgat
//
LOCUS       DAOMAA010000960         1088 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_184640_length_1088_cov_3.106486, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000960 DAOMAA010000000
VERSION     DAOMAA010000960.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_184640_length_1088_cov_3.106486"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1088
                     /locus_tag="PLO59_11065"
     CDS             <1..>1088
                     /locus_tag="PLO59_11065"
                     /inference="COORDINATES: protein motif:HMM:NF014869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HQV01694.1"
                     /translation="EMPLSHTCNQTTNPTVYNGNQTIKTTYSSFQTDYYLLDDCDATE
                     IYVMDAQISVGTSNAIDIWSGGASNTFTDKQGLAGAQTLWNLKNSVRYFKKRHGRDSY
                     DGTSTNLEAFVRAAFPVANQPNTGANAMYESFWEEFFFGYGLSDSAASDDYVVLDVAG
                     HEFTHAVISETAGLKYEREPGAINESFADIFGEVIERYTLGSCDMLHGVSYSLRNMLN
                     PQNSSFPQPASYYDTLWVATNCANPNVANDYCGVHFNSGVQNRMFALLCLGGSGTNFS
                     GDKYSVLPISVLKAGDIAYRALTVYLTSTSGYYDAREAWIKAAEDLYGNCSFEAIQVA
                     SAWHAVAVGHSLNQYNNGVCGVVQGSIV"
BASE COUNT          318 a          184 c          247 g          339 t
ORIGIN      
        1 gaaatgccgc ttagtcatac ctgtaaccaa acaaccaatc ctacagtata taatggcaat
       61 caaaccataa aaacaactta cagcagtttt caaaccgatt attacttatt ggatgattgt
      121 gatgcaacag aaatttatgt aatggatgca caaattagcg taggcacatc aaatgctatt
      181 gatatttgga gcggtggtgc cagcaacacg tttacagata aacaaggcct tgcaggcgca
      241 caaacattat ggaacttaaa aaacagtgtg cgttatttta aaaagcgtca tggacgcgat
      301 agctatgatg gtacatccac aaatttagaa gcttttgtac gtgcagcttt tccggtagca
      361 aatcaaccta atacaggtgc taatgcgatg tatgagagtt tttgggaaga gttctttttt
      421 ggatatgggt taagtgatag tgcagcttct gatgattatg tggtgcttga tgttgccgga
      481 cacgaattta cgcatgccgt aataagcgaa acggctggtt tgaaatatga acgcgaacct
      541 ggtgcaatta atgaatcgtt tgcagatatt tttggtgaag taattgagcg atacacttta
      601 ggcagttgcg atatgttaca tggtgtatct tattctttgc ggaatatgct aaacccgcaa
      661 aacagcagct ttccgcaacc tgctagttat tatgatactt tatgggtagc taccaattgt
      721 gcaaatccca atgtagcaaa cgattactgt ggcgtacatt ttaatagcgg tgtacaaaac
      781 cgcatgtttg ctttgttatg tttgggtggt tcgggcacca atttttcggg tgataagtat
      841 agtgttttgc ctatttcggt tttaaaagca ggagatatag cctatcgcgc attaaccgtt
      901 taccttactt caacttcagg atattatgat gcacgtgaag cttggataaa ggctgctgaa
      961 gatttatatg gtaattgttc ttttgaagca attcaagttg ctagtgcctg gcatgctgtt
     1021 gcagtagggc attcgctaaa ccaatataat aatggtgttt gtggagtggt gcaaggctca
     1081 attgtatt
//
LOCUS       DAOMAA010000961         1088 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_184655_length_1088_cov_2.944821, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000961 DAOMAA010000000
VERSION     DAOMAA010000961.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_184655_length_1088_cov_2.944821"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="PLO59_11070"
     CDS             <1..380
                     /locus_tag="PLO59_11070"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQV01695.1"
                     /translation="KNIDRDNTLLPPLILQPFVENAIWHGLQPKNQRGKITITISQTS
                     NQLLCVVEDNGIGRMAAMQKRTTNMQQHKKSLGMEITRQRIKLYNSLKNAKAYFDIVD
                     LLDAQQINAGMRVQLFLPFEEAN"
     gene            616..>1088
                     /locus_tag="PLO59_11075"
     CDS             616..>1088
                     /locus_tag="PLO59_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01696.1"
                     /translation="MFKIFNFIFCVLILQIQTSFAQCTGSELSGTVPIVFPTTDRLLT
                     FNIYHGFSLTPGTATLPVVKTYSYNHAFVTEMGSPCGAVAVNPAGQPAGYGAFRKDTL
                     IGDIMRNGDLPVTYKVVTVSPGGTVGTITDTAFAHTCISKSKASSCIDINPFGAGV"
BASE COUNT          382 a          222 c          192 g          292 t
ORIGIN      
        1 acaaaaacat tgacagggac aataccttat tgccgcctct aattttgcag ccttttgttg
       61 aaaatgcaat ttggcatgga ctacaaccca aaaaccaacg cggcaaaatt accattacca
      121 ttagccagac aagcaatcaa ttactatgtg tggttgaaga taatggtatt ggacgcatgg
      181 ctgccatgca aaagagaacc accaacatgc agcaacataa aaaatcgtta ggcatggaaa
      241 ttacacgcca acgtattaag ctttacaata gtttaaaaaa cgcaaaagcc tatttcgata
      301 tcgttgactt gttggatgca caacaaataa atgccggcat gcgcgtgcaa ttatttttac
      361 cattcgaaga agccaattag cataaaacgt caaaaataaa tcaaccggtt tcaatgttac
      421 accaaccaaa cacaaaacaa caacaaccaa aatcaaataa gcggtttgct gaaaacaaaa
      481 ttgtataatt atttgtaagc agcataaaac gcagaactta atttaataat gcatattttt
      541 aaataataaa ataaagacat aaaaaggaat taaggcaagc gagttaaaca tcttttaaag
      601 taaaaatcct atgccatgtt taaaattttc aacttcattt tttgtgtatt aatccttcaa
      661 atacaaacct cttttgcaca atgtaccgga agcgaattaa gcggtacggt accaattgta
      721 tttccaacaa ccgaccgcct gttaactttt aatatctatc acggattttc attaacaccg
      781 ggcacagcaa ccttgcctgt agtaaaaaca tacagttaca atcatgcatt tgtaactgaa
      841 atggggtcgc cctgtggcgc tgtagctgta aatccagcgg gccagcctgc cggttatggc
      901 gcttttagaa aagatacact tataggcgat atcatgcgaa atggcgactt gcctgttacg
      961 tacaaagtag taacggtttc gcccggtggt acggttggta ctataacgga tacggccttt
     1021 gcgcatacct gcatttcaaa atcaaaagca agctcatgta ttgatattaa tccctttggt
     1081 gccggtgt
//
LOCUS       DAOMAA010000962         1086 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_185315_length_1086_cov_3.400582, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000962 DAOMAA010000000
VERSION     DAOMAA010000962.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_185315_length_1086_cov_3.400582"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..>1086)
                     /gene="ribA"
                     /locus_tag="PLO59_11080"
     CDS             complement(118..>1086)
                     /gene="ribA"
                     /locus_tag="PLO59_11080"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HQV01697.1"
                     /translation="SMHETPFTVSVDLLGHGCTTGISTADRSKTIKALVDPTIKPEEL
                     GRPGHIFPLRARNGGVLRRSGHTEAVVDLARIAGFEPAGVLVEIMNEDGTMARLPQLY
                     KIADKFDLKIISIEDLISYRLKTESLIEKQVVVELPTAYGNFELHAYKQINTDQNHVA
                     IVKGNWKKDEAILIRVHSSCLTGDVFGSCRCDCGPQLQKAMQMIEQAGKGVIVYMNQE
                     GRGIGLLNKLKAYKLQEMGRDTVEANLELGFKIDERDYGVGAQILRDLGITKMKLMTN
                     NPTKRAGLIGYGLEIVENVSIIAGANLHNKEYLETKQNKMGHSLNL"
BASE COUNT          313 a          230 c          181 g          362 t
ORIGIN      
        1 tgtgcttgca gcctaaaagc taataaggtt agtagtataa aaaggtactt tgtcatgaag
       61 cgcaatttgt acaataaacg ttgcagttaa aatttatttt agcggcagga attaatatta
      121 taaattcaaa ctgtgtccca ttttattttg tttggtttcc aaatattctt tgttatgcaa
      181 attagccccg gctataatcg aaacattttc tactatctct aatccatatc caattaaacc
      241 tgctcttttg gtaggattgt tagtcattaa tttcatttta gtgataccta aatctcttaa
      301 aatttgagca ccaacaccat aatcacgttc atcaatttta aaacctaatt ccaggttggc
      361 ttcaacagta tcgcgtccca tttcctgaag cttgtatgct tttagtttat ttaataatcc
      421 gataccgcgg ccttcctgat tcatataaac aataacacct ttgccagcct gttcaatcat
      481 ttgcatggct ttttgtaatt gagggccgca atcgcagcgg catgagccaa aaacatcacc
      541 cgttaggcat gatgaatgta cacgtatcaa aattgcttca tcttttttcc aattaccttt
      601 tacaatagcc acatgatttt gatcggtatt aatttgctta taagcatgca actcgaaatt
      661 gccataagcg gttggtagtt caactaccac ttgcttttca attaacgatt cggttttaag
      721 cctgtatgaa attaaatctt ctattgaaat tattttcaaa tcaaacttat cggcaatttt
      781 atagagttgc ggtaagcgtg ccatcgttcc atcttcattc ataatttcta ctaaaacacc
      841 tgcgggttca aagcctgcaa tacgtgccag gtcaaccact gcttcggtat gacccgagcg
      901 tcttaaaact ccgccattac gtgcacgcaa cggaaatata tggccgggcc gacccaactc
      961 ttcaggctta attgtagggt caaccaatgc tttaattgtt ttcgatctgt cggctgtact
     1021 tattccggta gtacaaccat ggcctaataa atcaaccgaa acagtaaagg gtgtttcgtg
     1081 catgct
//
LOCUS       DAOMAA010000963         1085 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_185846_length_1085_cov_1.917476, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000963 DAOMAA010000000
VERSION     DAOMAA010000963.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_185846_length_1085_cov_1.917476"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="PLO59_11085"
     CDS             complement(<1..188)
                     /locus_tag="PLO59_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01698.1"
                     /translation="MRKIITMAMRKRIISNRKSDNDTASHNRTTNKPEKAGSSRSYKK
                     TNFTKQGDDANERSYGDKP"
     gene            complement(216..932)
                     /locus_tag="PLO59_11090"
     CDS             complement(216..932)
                     /locus_tag="PLO59_11090"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HQV01699.1"
                     /translation="MKKLTTIVILLFLVTTLQAQTEKMNTVKRIEVSGSAELELVPDE
                     IFVNFTLQEFYDNQKKKHDIDEIQTTFLNTCAKFGITKDRIQIKSMSGFNGNNWYWRK
                     QKREQPDLLANTVYIIKFSNAADIDKLVNNMDDRATNQLYISKTSHSKMDEYKRDLKI
                     KALQAAKNKATYLCEAVGEKVGQALIISEFENNFEPQYAQPKMYTNMAMDMAAGSANE
                     GVDFQKIIVRSEIKATFAIQ"
BASE COUNT          264 a          198 c          196 g          427 t
ORIGIN      
        1 ggcttatcgc catagctgcg ctcgttggca tcatcaccct gtttggtgaa atttgttttt
       61 ttatacgacc tgctgctgcc agctttttcg ggcttattgg ttgtacggtt atgtgatgcc
      121 gtgtcgttat ctgacttacg gttgctgatg atgcgtttgc gcattgccat ggtaataatt
      181 ttgcgcaaag atattattta agagctagct tacttttatt gaatggcaaa cgtggcctta
      241 atttcgctgc gaacgataat tttttgaaaa tcaacacctt catttgcact gcctgcagcc
      301 atatccatag ccatgttggt gtacattttt ggttgcgcat actgaggttc gaaattgttt
      361 tcaaactcgc ttattatcaa tgcctgtcct actttttcac caacggcttc gcataagtaa
      421 gttgccttgt tttttgctgc ctgtaaggct ttaattttta aatcgcgttt gtattcatcc
      481 attttactat gcgatgtttt gctgatatat aactggttag tggctctatc gtccatatta
      541 tttaccagct tatcaatatc ggccgcattg ctaaatttta taatgtaaac agtattagcc
      601 agtaaatcgg gttgctcgcg tttttgtttg cgccaatacc aattgttgcc attaaatcca
      661 ctcatgcttt taatttgtat gcgatctttg gttatgccaa acttggcaca agtgtttaaa
      721 aaagttgttt gtatttcgtc aatatcatgt ttcttttttt gattgtcgta aaactcctgc
      781 aacgtaaaat ttacaaatat ttcatcgggt accagttcta attcggccga gcccgatact
      841 tcaattcgtt ttactgtatt cattttttct gtttgtgctt gtagcgttgt tactaaaaat
      901 aatagtatca ctatggttgt tagttttttc atcgtttaat tataatttat cgtgaagatg
      961 cataagtgta attaaatgca gaaactttta aaatattatt cggcttgtgg gtaaaatgca
     1021 tcttctacat gtattatttc tgttccactt tttgttttaa taattgcaac aacatcaaaa
     1081 cgtgc
//
LOCUS       DAOMAA010000964         1084 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_186012_length_1084_cov_2.894072, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000964 DAOMAA010000000
VERSION     DAOMAA010000964.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_186012_length_1084_cov_2.894072"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="PLO59_11095"
     CDS             <1..335
                     /locus_tag="PLO59_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01700.1"
                     /translation="TNAYINEVNETVTAWFLEMNKSNVYLIKKTVRKTIRLIDKKIKF
                     AALPKVTVEICMHWCQCFLANNFHKKQATVLDNLYISQVKKMEKAYNLLHPDVQMDYS
                     AQMQRFIK"
     gene            393..>1084
                     /locus_tag="PLO59_11100"
     CDS             393..>1084
                     /locus_tag="PLO59_11100"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004365 - glyceraldehyde-3-phosphate
                     dehydrogenase (NAD+) (phosphorylating) activity [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HQV01701.1"
                     /translation="MIHIAINGFGRIGRTLLRQLLQMPQIKIVAINDLADAQTLAHLF
                     KYDSIHGLFDGTVASQAHAFVINEQEIPLYNEREPQNLPWKQLDVDILIECSGKFTSY
                     DKAILHHHAGARKVIISAPATGNLKSVVIGVNEQILDGSEIVVSNASCTTNNAAPMLK
                     VLNDNWGIESAFITTVHAFTGDQNLHDAPHKDLRRARAATQSIIPTTTGAAKAITDIF
                     PEMKGHIGGAGI"
BASE COUNT          381 a          186 c          212 g          305 t
ORIGIN      
        1 atacgaatgc atatataaac gaggtaaatg aaactgtaac cgcatggttt ctcgaaatga
       61 ataaaagcaa tgtgtatctg ataaaaaaaa cagtgcgtaa aaccatcagg cttattgata
      121 aaaaaataaa gtttgcggca ttacctaaag taactgttga aatttgcatg cattggtgcc
      181 aatgtttttt agcaaacaac tttcataaga agcaagcaac tgtgcttgat aacttataca
      241 taagccaggt taaaaaaatg gaaaaagcat ataatttatt gcatcccgat gtgcaaatgg
      301 attatagtgc gcaaatgcaa cgatttatca aataagatag aaacttaaac gttttgaatt
      361 tatgttacaa ttagattttt aaaattatta atatgattca catagctata aacggatttg
      421 gtaggatagg ccgcacattg ctgcgacaac ttttgcaaat gccgcaaatt aaaattgttg
      481 ccattaatga tttagccgat gcccaaacgc ttgcacattt atttaaatac gattcgatac
      541 atggtttatt tgatggaaca gttgcaagcc aagcccatgc ctttgtaatt aatgagcagg
      601 aaattccatt atataatgag cgcgaaccac aaaatctgcc ctggaaacag ttggatgttg
      661 atatattgat tgagtgttcg ggtaaattta catcatacga taaagcgata ttgcatcatc
      721 atgcaggtgc ccgcaaagta attattagtg ctccggcaac aggcaactta aaatcggtag
      781 taataggtgt aaacgaacaa attttagatg ggtcagaaat agtggtttcc aatgcatcct
      841 gtacaaccaa taatgcagca cccatgctaa aagtgctcaa tgataactgg ggtattgaaa
      901 gtgcgtttat tactaccgtt catgcattta ccggagatca gaatttgcat gatgcaccgc
      961 ataaggattt gcgaagggcg cgtgcagcaa cacaatccat tataccaact acaacaggag
     1021 ctgctaaagc cattaccgat atttttcctg aaatgaaagg ccacattggt ggtgcaggca
     1081 taag
//
LOCUS       DAOMAA010000965         1084 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_186022_length_1084_cov_2.812439, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000965 DAOMAA010000000
VERSION     DAOMAA010000965.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_186022_length_1084_cov_2.812439"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="PLO59_11105"
     CDS             complement(<1..305)
                     /locus_tag="PLO59_11105"
                     /inference="COORDINATES: protein motif:HMM:NF013663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HQV01702.1"
                     /translation="MRIAVYGRQQSTSVIPYVEQLMVALESHQCEILLYQPFLHFLQK
                     HSSKKYKHQTFSSNTDLRHQVDVIFSVGGDGTMLDTLTLVQDSMIPVAGVNTGRLGF"
     gene            complement(386..>1084)
                     /locus_tag="PLO59_11110"
     CDS             complement(386..>1084)
                     /locus_tag="PLO59_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01703.1"
                     /translation="EYRYASIDSSVLSLNENYFLPHQTYSQYIALNYKFVQDKRDYRL
                     YALKGSYFDFELGKVGLGLLPNEPNLFFVTSTYKNTLKLAHRWHYSYSIKGRVAGPQQ
                     APYYNQRALGYFNDIVRTYELYVINGQHYALFKSNLKFTLLPQQIVQLPVITDPKFGL
                     IPFAFYLNLFADLGYVYDDVFASTNPLANVLLPGVGIGLDLVTYYNAVIRLEYGINRR
                     GETGLFLNFTNALY"
BASE COUNT          343 a          194 c          213 g          334 t
ORIGIN      
        1 aaaaacccca gccggcctgt gtttacacct gcaacaggta tcatcgaatc ttgcaccaag
       61 gttagggtat cgagcatggt accatcgccc ccaacactaa aaattacatc aacctgatgg
      121 cgcaaatcgg tgttcgaact aaaggtttgg tgtttgtatt ttttggaact gtgtttttgt
      181 aaaaaatgta aaaacggttg atatagtaat atttcacatt gatgcgattc gagcgctacc
      241 attaattgct ccacataagg aattactgaa gtagattgtt gacggccata aactgctatg
      301 cgcataaggt gcttttattt tatactgttt ataaaaagca aatgtaagct aatgcactta
      361 ctattaaatt aaaacatatc tgccactaat acaatgcatt ggtaaagttt aaaaataaac
      421 cggtttcgcc acgcctgttt atgccatact ccaggcgtat tactgcattg taataggtta
      481 ctaaatctaa accaatacct acaccgggca gcagtacatt tgccaatgga ttggtgcttg
      541 caaaaacatc atcatatacg taacccaaat cggcaaaaag attcagataa aatgcaaatg
      601 gaataaggcc aaactttggg tcggtaataa caggcagttg cactatttgt tgtggtaata
      661 atgtaaattt aaggttggat ttaaacaagg cataatgttg cccgtttata acatacaact
      721 cataggtgcg tacaatgtcg ttaaagtatc ctaatgcgcg ttggttatag tatggtgctt
      781 gttgtggtcc ggcaacacgt ccttttattg aataactgta atgccagcga tgggctaatt
      841 ttaaagtgtt tttataggtg ctggttacaa aaaataagtt gggttcgttg ggcaataaac
      901 ccaagcccac tttacccaat tcaaaatcga agtaactgcc tttaagtgca tataatctgt
      961 agtcgcgctt gtcttgtaca aacttatagt ttagggctat gtattgtgaa taggtttgat
     1021 ggggtaaaaa ataattttcg tttaacgata gtacactact atcaattgaa gcgtatctgt
     1081 actc
//
LOCUS       DAOMAA010000966         1083 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_186348_length_1083_cov_3.056420, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000966 DAOMAA010000000
VERSION     DAOMAA010000966.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_186348_length_1083_cov_3.056420"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="PLO59_11115"
     CDS             complement(<1..619)
                     /locus_tag="PLO59_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01704.1"
                     /translation="MALSRVWSYLILFSIVVACYQYFKGDKNIFNDVVIERKLKDNET
                     FGKVQYSVIDNAAITVSANKLAKANWHVSENVSTSDFIVCNNAYNKHAALQLKLETIP
                     IVTTTNIQPAKFGKWALLDVDSASVDTIITQVALTNIYSADAIICNHSNTDSIRNALI
                     AKLNQQKIITAHEVALYLPKNIDGIFETAKFAITVVIGLIGILALF"
     gene            complement(720..>1083)
                     /locus_tag="PLO59_11120"
     CDS             complement(720..>1083)
                     /locus_tag="PLO59_11120"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQV01705.1"
                     /translation="DIAFSPNVVSNLIAEFYVIKNLSAQVIGQYIGKQFLDNTANSNK
                     ALAAYGLINLRAFYKIDALKKIPALDLGLQVNNLLNHSYSANGYTYSYISGGTAVAEN
                     FYFPQAGTQWMLQLKVLF"
BASE COUNT          338 a          196 c          188 g          361 t
ORIGIN      
        1 taaacaaggc aagtatgcct attaacccta ttacaacggt tatggcaaac tttgccgttt
       61 cgaaaatgcc gtcaatgttt tttggtaagt acaatgctac ttcatgcgca gtaataattt
      121 tttgctgatt aagttttgca attaaagcat tacgtataga gtctgtgtta ctgtgattac
      181 aaattattgc atcggcacta taaatatttg taagtgcaac ttgtgtaatt attgtatcaa
      241 cacttgcact atccacatct aacaatgccc acttaccaaa tttagcaggt tgtatgttag
      301 tagttgttac aatcggtatg gtttctaact ttaattgcaa ggcggcatgt ttgttatagg
      361 cattgttaca aacaataaaa tcgcttgtac ttacattttc agatacatgc caattggctt
      421 ttgcgagttt gttggcacta acggttatgg ctgcattatc aattaccgaa tattgaactt
      481 taccaaaggt ttcattatcc ttcaatttac gttcgataac tacatcgtta aaaatatttt
      541 tatcgccctt aaaatattga tagcatgcca ccactatcga aaataaaata aggtaagacc
      601 aaaccctgct taatgccata atgtaaacac tttaggccga taaatgtaaa cgctttttat
      661 gcatactgct aagggcttgc atgatgatat aaacaaaact gttttaaaaa cccaaccgtt
      721 taaaacaata cttttaattg cagcatccat tgggtaccgg cttgaggaaa ataaaaattt
      781 tcggcaaccg ctgtgccgcc acttatgtat gagtaggtgt aaccattggc actgtaggag
      841 tggttaagca aattgtttac ctgtaaacct aagtctaaag ccggtatttt ttttaaggca
      901 tctattttat aaaaagcgcg caggtttatt aagccgtatg ctgccaaagc tttgttactg
      961 ttggctgtgt tatccagaaa ctgtttgcca atgtattggc cgataacttg tgccgacaaa
     1021 ttttttatca cataaaactc tgcaattaaa ttacttacaa cattgggcga aaatgcaatg
     1081 tca
//
LOCUS       DAOMAA010000967         1078 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_187995_length_1078_cov_3.149560, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000967 DAOMAA010000000
VERSION     DAOMAA010000967.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_187995_length_1078_cov_3.149560"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="PLO59_11125"
     CDS             complement(<1..282)
                     /locus_tag="PLO59_11125"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQV01706.1"
                     /translation="MIKYNKMLLLVVVLFWQQSAFAQQKWSLKQCVDYAIENNISIKQ
                     SYINTEVARTNADQNFASMFPNVNASASQSFNFGRSIDPTTNVYINSNIN"
     gene            482..>1078
                     /locus_tag="PLO59_11130"
     CDS             482..>1078
                     /locus_tag="PLO59_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020600912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQV01707.1"
                     /translation="MINSKKIVVVLPAYNAALTLAQTLSEIPKPLVDEVVLVDDCSTD
                     NTIEVAQNLGITNIIKHQKNKGYGGNQKTCYDKALQLDADIIIMLHPDYQYTPLLITP
                     MAHIIASGVYQVVLGSRILGKGAIKGGMPLYKYFFNRMLTLVQNILMNQKLSEYHTGY
                     RAYSKAVLSRINYHANSNDFVFDNQLLSQIFFAGFEIAE"
BASE COUNT          333 a          182 c          200 g          363 t
ORIGIN      
        1 attaatgttg ctgtttatat atacgttggt ggtggggtca atagatcggc caaagttaaa
       61 cgactggctg gcacttgcat ttacgtttgg aaacatagat gcaaaatttt ggtcggcatt
      121 ggtgcgtgcc acttcggtat ttatatacga ttgttttata ctgatgttgt tttcaatcgc
      181 ataatcaacg cattgtttta aactccattt ttgctgggca aaagcacttt gttgccaaaa
      241 taaaacaact actaaaagca gcatcttgtt atactttatc atgggtctgg tttaaatttg
      301 tttttgttgg gtttatttaa gtgcaagtaa ataggtaaca gggttaccca attttttttt
      361 ctgttaagct ttattaaacg ttaattgacg atttaggttg ttttttgtta catctattaa
      421 atttttatca ccttggccaa aacacttatg gtattagcta catatagctt ttaagatatt
      481 tatgattaat agtaaaaaaa ttgtggtggt actgcccgct tacaatgcag ctttaacact
      541 ggcacaaacc ctttccgaaa ttccgaaacc cttagttgat gaggtggttt tggttgacga
      601 ttgcagtaca gataatacca tagaagtagc tcaaaattta ggcattacaa atattataaa
      661 acaccaaaaa aacaaaggct atggtggcaa tcaaaaaacc tgttacgata aagcactgca
      721 attagatgcc gatataataa taatgctcca tcccgattat caatacactc cgctgcttat
      781 tacacccatg gcacatatta ttgcaagtgg tgtgtatcag gtggtattag gctcgcgtat
      841 tttaggcaag ggtgcaataa aaggcggcat gccattgtat aaatactttt ttaaccgcat
      901 gcttactttg gttcaaaaca tattgatgaa tcaaaaatta agtgagtatc acacaggcta
      961 ccgtgcctat agtaaagccg ttttaagtcg cataaattat catgcaaaca gcaacgactt
     1021 tgtattcgat aatcaactgt tgtcacaaat attttttgcc ggtttcgaaa ttgccgaa
//
LOCUS       DAOMAA010000968         1078 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_188020_length_1078_cov_2.805474, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000968 DAOMAA010000000
VERSION     DAOMAA010000968.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_188020_length_1078_cov_2.805474"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="PLO59_11135"
     CDS             <1..514
                     /locus_tag="PLO59_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01708.1"
                     /translation="INSTVIKDAQNAVFVMPDGKLDISEATLDRNYIGINIPHLNNGL
                     INVTGTTFSCTGCLTGISLKQSSTGYLGNVTRAGMIINDGFYSIGNAQSGTNTFKELY
                     RGIESHSSICKIANTTFENFPSQNEYIELPTDPGGGIAIYASNTPTGTMSITGLGIGT
                     ASPVIFTNCF"
     gene            518..>1078
                     /locus_tag="PLO59_11140"
     CDS             518..>1078
                     /locus_tag="PLO59_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01709.1"
                     /translation="MFAKGGHVTATLIKMHNMTYGITARENDWSNVNITANILTDVRN
                     TGVQSFLNETANTINITSNTITTTGSVAKGILISERGTAVQGRQLHIETNIITTTNGG
                     SGIECSNVNHARLLYNSITQDESSSNTNAQYGIKLNGNETTQLNCNSVTRNNSSTAVN
                     FGYHINISTSNNLVCNTANNNDLGIYF"
BASE COUNT          384 a          227 c          221 g          246 t
ORIGIN      
        1 tataaacagt acagttatta aagatgcgca aaatgcagta tttgttatgc ccgatggcaa
       61 attggatata agcgaagcta ccctggatag aaattatatt ggcataaaca taccccacct
      121 aaacaacgga ttaataaacg ttaccggtac cacctttagt tgcacgggct gcctcacagg
      181 aatctcactt aagcaaagca gcacggggta tttaggcaat gtaacacgtg ccggtatgat
      241 aattaatgat gggttttact ctattggcaa tgcccaaagc ggcaccaata cttttaaaga
      301 attgtatcgt ggtattgaaa gccacagcag tatttgtaaa atcgcaaaca ccacatttga
      361 aaacttccca tcacaaaatg aatatataga gctacccacc gacccagggg gaggcatagc
      421 catatacgca agcaatacgc caacaggcac aatgagtata acgggcttag gcattggaac
      481 ggcaagcccg gttatattta ccaattgttt ctagggtgtg tttgcaaagg gcggccatgt
      541 aaccgccaca ctaatcaaaa tgcacaacat gacgtatggc ataacggcac gcgaaaatga
      601 ctggagtaat gtaaacatca cggccaatat acttaccgat gtaagaaaca caggtgtgca
      661 aagttttctg aacgaaacag ccaatactat aaatataaca agcaatacga ttacaacaac
      721 cggtagtgtt gccaagggca ttttaattag tgaacggggc acagcggtgc aaggccgtca
      781 gttgcatatt gaaaccaata ttattactac caccaacggt ggcagcggta tagagtgcag
      841 caatgtaaac catgcaaggc tgctgtataa cagcataaca caggatgaaa gcagcagtaa
      901 taccaatgca caatatggca taaaactaaa cggcaatgaa accacacaat taaattgcaa
      961 cagtgttacg cgcaacaaca gtagtacagc agttaacttt ggctaccata taaacataag
     1021 caccagtaac aacctggtat gcaatacggc caataataac gatttaggaa tttatttt
//
LOCUS       DAOMAA010000969         1078 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_188191_length_1078_cov_1.839687, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000969 DAOMAA010000000
VERSION     DAOMAA010000969.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_188191_length_1078_cov_1.839687"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..758
                     /locus_tag="PLO59_11145"
     CDS             <1..758
                     /locus_tag="PLO59_11145"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HQV01710.1"
                     /translation="SIKIAAVSYLNTKPFLYGLQQYKNDLNIEINSYEPHICAQQLMQ
                     NHAQIGLIPVAALPAIPQARIISNYCIGANKEVDSVLIVSKVPIKMVTHLYLDYQSRT
                     SVLLAQILLKFFWQKEVILLAANPGYENEINHTTAGVIIGDRALRLKAQFEFVYDLAQ
                     AWHQYKKLPFMFAAWVSTLPVSNSFETKFNQACAAGLAAIPAINEALQKQANYYPNTL
                     DYLSHKISYQRNAAKMEALKQFFELIKLLPVSA"
     gene            899..>1078
                     /locus_tag="PLO59_11150"
     CDS             899..>1078
                     /locus_tag="PLO59_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007292454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HQV01711.1"
                     /translation="MKTYDEKHIKNIVLAGAPKSGKTTLCETMLFEAGIIQRRGTVEE
                     KNTVSDYHEIEQQRGS"
BASE COUNT          370 a          215 c          186 g          307 t
ORIGIN      
        1 tgtctatcaa aatagcagcc gtttcatatc ttaatactaa accattttta tatggcttgc
       61 agcaatataa aaatgattta aatattgaaa taaacagtta tgaaccgcat atatgcgcac
      121 aacaactaat gcaaaaccat gcgcaaattg gtttgatacc ggtagctgca ttacccgcta
      181 taccacaagc ccggattatt agtaattatt gcatcggtgc caataaagaa gtggactccg
      241 tattaatagt aagcaaagtg ccaattaaga tggttacaca tttgtattta gattatcaat
      301 cgcgtacatc ggtattgtta gcacaaatac ttttaaagtt tttctggcaa aaggaggtaa
      361 ttttacttgc agcaaacccc ggctacgaaa atgaaataaa ccatactaca gccggtgtaa
      421 ttattggcga ccgcgccctc aggcttaaag ctcagttcga atttgtttat gacttggcac
      481 aagcatggca tcaatataaa aaactaccat ttatgtttgc tgcctgggta agtaccttgc
      541 ccgtttcaaa ttcttttgaa actaaattta atcaggcatg tgcagcaggt ttagcagcca
      601 ttccggcaat taacgaggca ttacaaaagc aggccaacta ctaccccaac accttagatt
      661 acttgtcgca taaaatcagc taccaacgca atgcagccaa aatggaagct ttgaaacaat
      721 ttttcgagtt gataaaactt ttacctgtat ctgcttaaac attcaggtgc ttttacaaaa
      781 agacaagcta ttttattttt atcattgtat gttatggttt aatgccctac tttaggacgg
      841 tcgcgttctt tacaggcata aaattactat ttatcatcaa acactaaaac catgttatat
      901 gaaaacatac gatgaaaaac atatcaaaaa cattgtgctg gcaggtgccc caaaatcagg
      961 taaaacaaca ctgtgcgaaa ccatgttatt tgaagccggc attattcaac gcagaggcac
     1021 cgttgaagaa aaaaacaccg taagcgatta tcatgaaatt gaacaacaac gtggcagt
//
LOCUS       DAOMAA010000970         1077 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_188327_length_1077_cov_3.592955, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000970 DAOMAA010000000
VERSION     DAOMAA010000970.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_188327_length_1077_cov_3.592955"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="PLO59_11155"
     CDS             <1..209
                     /locus_tag="PLO59_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015908579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HQV01712.1"
                     /translation="LEKNLKVEKITLDELETAAREHGEANLKNVDLAVLERDGSISII
                     SKSYQHRTNKKRKVYKPFTQSNES"
     gene            228..>1077
                     /locus_tag="PLO59_11160"
     CDS             228..>1077
                     /locus_tag="PLO59_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01713.1"
                     /translation="MRFVILYALFCLSVNLQAQFHSEKLLRPQLMVTYENRFTQSYNQ
                     SDKNLSFLQYFGSFSYPVINRINKANTQGTFKFTGLTLFVQAARNNMNDDLLGDSRNL
                     YQTQAGFDFIFIPGNKFYYQLTGKAHRLCDNKRPDINLQYTGLALLHYFIKPVTLHGG
                     IARTMALGHMQLTPVGGISIYWKPNWQLHILYPFIAQLRKNISTQMAVALFVKADGML
                     YDITNENNFANAGSTIALEQKVYAGGLQLNYDFDDHWQVFAEGGSLFNAAHAYKFEKL
                     TLVQSNL"
BASE COUNT          362 a          202 c          205 g          308 t
ORIGIN      
        1 tgttagagaa aaacctgaaa gttgaaaaaa ttacgctcga tgaattagaa actgcagcgc
       61 gcgagcatgg cgaagcaaac cttaaaaacg ttgacctggc agtacttgaa cgcgatggca
      121 gcataagcat tatcagcaaa agctaccaac accgaaccaa taaaaaacga aaggtgtata
      181 aaccgtttac tcaatcaaac gaaagttaaa tgtaactatt accatatatg cggtttgtaa
      241 ttttatatgc actattttgt ttatcggtaa atttgcaggc acaatttcat agcgaaaagt
      301 tgctgcggcc acagcttatg gttacttacg aaaaccggtt tacacaatcg tataatcaaa
      361 gcgataaaaa tctttcgttt ttacaatact ttggcagttt tagttatccg gttataaacc
      421 gaataaataa agctaacaca caaggcacat ttaaatttac agggcttaca ttgtttgtgc
      481 aagcagcccg caataatatg aatgacgatt tgctgggcga tagccgcaac ctgtatcaaa
      541 cccaggccgg atttgatttt atttttatac cgggcaacaa attttattat cagcttactg
      601 gtaaagccca tcgtttatgc gataataaaa gaccagatat taacctgcaa tacaccggtt
      661 tggcattgct gcattatttt attaagccgg taacattaca tggtggcatt gcacgtacta
      721 tggctttggg ccacatgcaa ttaacacctg taggtggcat cagcatatat tggaaaccca
      781 actggcaact gcatatttta tatccattta tagcgcaact gcgcaaaaat ataagtacac
      841 aaatggctgt ggcacttttt gttaaagcag atggtatgct atacgatatt accaatgaaa
      901 ataattttgc taatgccgga agtaccattg cattagaaca aaaagtatat gcaggcggct
      961 tacagttgaa ttatgatttt gacgaccatt ggcaagtgtt tgccgaaggt ggcagcttgt
     1021 ttaatgccgc ccatgcttat aaatttgaaa aactaacatt ggtacaatca aatttaa
//
LOCUS       DAOMAA010000971         1077 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_188424_length_1077_cov_2.318004, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000971 DAOMAA010000000
VERSION     DAOMAA010000971.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_188424_length_1077_cov_2.318004"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="PLO59_11165"
     CDS             complement(<1..751)
                     /locus_tag="PLO59_11165"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HQV01714.1"
                     /translation="MKFKFNLLVLATTVLLQQNLFAQTVKTKNAAAKYKPQTVVIKVL
                     PQLRHLCENEKINDYKLQQVFSELGLAHVTKKFKNHRRPETAYNAQQQPLVDLSLIYQ
                     LQYNGKLTVQQAIKKLMATGHLQYADPHYIYQTFYTPNDPQLNNQYFFTNINAYNAWD
                     IQKGDTNVVVGIVDSGSDPNHPDLAANLKHNYADPVNGLDDDADGFIDNFSGWDVSEN
                     DNDATVGSEPHGSHVSGCAAAGNSCCVTHFPV"
     gene            complement(865..>1077)
                     /locus_tag="PLO59_11170"
     CDS             complement(865..>1077)
                     /locus_tag="PLO59_11170"
                     /inference="COORDINATES: protein motif:HMM:NF024976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HQV01715.1"
                     /translation="IHNRMGKPWMLIECKAPEVKLNQNAFQQALRYHLNLSVQYLVLS
                     NGMQHFCFEVCNNELLLQKNMPAFPL"
BASE COUNT          303 a          183 c          230 g          361 t
ORIGIN      
        1 ttacggggaa gtgagtgaca caacaactgt tgccagcagc agcacagccc gatacatgtg
       61 agccatgtgg ctcggaacct acggttgcat cgttatcatt ttcgcttaca tcccaacccg
      121 aaaaattatc aataaatcca tcggcatcat cgtctaaacc atttacaggg tcggcataat
      181 tgtgttttag gttagcagct aaatcggggt ggttggggtc gctgcccgag tcaacaatgc
      241 ctaccactac attggtgtca cctttttgaa tatcccaggc attgtatgca ttgatatttg
      301 taaaaaagta ttgattgttt aattgcgggt cgttaggtgt ataaaaagtt tgatagatgt
      361 aatgcggatc ggcatattgc aaatggcccg tagccataag ttttttaatg gcctgttgta
      421 cggtaagttt accgttgtat tgtagttgat aaattaagct taaatcaacc aacggttgtt
      481 gttgcgcatt gtaggctgtt tcgggcctgc ggtgattttt aaattttttg gtaacatgtg
      541 ctaagccgag ttcggaaaat acttgttgca atttatagtc gttaattttt tcattttcgc
      601 atagatgcct taattgtggc agcaccttaa taacaaccgt ttgtggtttg tatttagcgg
      661 ctgcattttt tgttttcact gtttgtgcaa acaaattttg ctgtagtaaa acggttgtgg
      721 ctaatacaag aaggttaaat ttaaatttca tagggatggg ttttaagcgt ggcgaatgta
      781 atttatttcg gcaagtcaaa ttttattacc gaatttaaag cctttcggaa aaattaattt
      841 acataaaaat gaaacatgat tgcattacag cggaaaagca ggcatgtttt tttggagcaa
      901 caactcgtta ttacatacct caaaacaaaa gtgttgcata ccattactta gtactaaata
      961 ttgaaccgat aaattcaaat gatagcgaag tgcctgttga aaggcatttt gatttagctt
     1021 tacttccggt gctttgcatt cgataagcat ccagggtttg cccattctat tgtgaat
//
LOCUS       DAOMAA010000972         1076 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_188664_length_1076_cov_3.475024, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000972 DAOMAA010000000
VERSION     DAOMAA010000972.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_188664_length_1076_cov_3.475024"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1076
                     /locus_tag="PLO59_11175"
     CDS             <1..>1076
                     /locus_tag="PLO59_11175"
                     /inference="COORDINATES: protein motif:HMM:NF026035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protease"
                     /protein_id="HQV01716.1"
                     /translation="AYISDVNGEDEVYIQQANETGTAKQLTKNGDTYKYAIDWSPDSK
                     YIAWSDNKLRLFYVNVETGNITLADQGKTWEHNSWNWSPDSRWLAFSRSDDDRVIKIF
                     LHNLATKENTLVTDAWYESHSPSFSDDGKFLLFVSDRDFNPTYSNTEWNHAYSNMSKV
                     YMMALTKKTPSPFSPKNDEVREVTESKPEDEKSNTNKPSDKTDKPKEGINNPVDIDFD
                     GIQGRIVSLPISAANYWHANMVGNMVYYMHSKTGADDVTLKVYDLKERKETEVGQVSS
                     YGLTADNKKMLINKDSKYYIIELPKSKAELKDAVDLSAMRIFVNRKQEWNQIYNESWR
                     QMRDFFWDPTMHGVDWAAMRTKYQ"
BASE COUNT          375 a          178 c          235 g          288 t
ORIGIN      
        1 ttgcgtatat aagtgatgtt aacggtgaag acgaagtgta tattcaacaa gcaaatgaaa
       61 caggtactgc aaaacaactg actaaaaatg gtgatacgta taagtatgct atcgattggt
      121 cgccagatag caaatacatt gcctggtcgg ataataaact tcgcctgttt tatgtaaatg
      181 tagaaacggg caacatcacc ttagccgacc agggaaaaac ctgggaacat aatagttgga
      241 actggtcgcc cgacagccgt tggctggctt ttagcaggag tgatgatgat agggttataa
      301 aaatattttt acacaacttg gctactaaag aaaatacttt ggttaccgat gcttggtacg
      361 aatcgcacag tccatcgttt agtgatgatg gaaagttttt gctttttgtt tctgatcgtg
      421 attttaatcc tacttatagc aatactgaat ggaaccatgc atattcaaac atgtcgaaag
      481 tgtatatgat ggcattaaca aaaaaaacac cctcgccatt tagtcctaag aatgatgaag
      541 tacgtgaggt aactgaaagc aaacctgaag atgaaaaatc aaataccaat aaaccatctg
      601 acaaaacaga taaaccgaag gaaggaatta ataatcctgt agatatagat ttcgatggca
      661 tacaaggccg cattgtttcg ttgcctataa gtgcggcaaa ttactggcat gccaatatgg
      721 tgggcaacat ggtatattat atgcactcga aaaccggtgc tgatgatgtt acacttaaag
      781 tatatgattt aaaagaaagg aaggaaacag aagtagggca ggtaagcagt tatggtttaa
      841 cagccgacaa taaaaagatg ttgattaata aggatagcaa atactatatt atagaattac
      901 ccaaaagtaa agctgagcta aaagatgctg ttgacctgag tgctatgcgc atttttgtta
      961 atcgtaaaca agaatggaat cagatttata atgaatcgtg gcggcaaatg cgcgactttt
     1021 tttgggaccc aaccatgcac ggtgttgatt gggctgccat gcgtaccaaa taccaa
//
LOCUS       DAOMAA010000973         1076 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_188803_length_1076_cov_2.131244, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000973 DAOMAA010000000
VERSION     DAOMAA010000973.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_188803_length_1076_cov_2.131244"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1030)
                     /locus_tag="PLO59_11180"
     CDS             complement(<1..1030)
                     /locus_tag="PLO59_11180"
                     /inference="COORDINATES: protein motif:HMM:NF018208.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01717.1"
                     /translation="MQVHKPQTAPPNSIPALSVVEFDKIYEPLMHYLLGHVYLVADNK
                     QVTDYIFEADSRLVLLSTSGTLLKQHYTVSGGSVGLFDGKRTGKFKNLQTLEETIKTL
                     AAQVETIQTDILATEKLFATANANLRETGQRLNAAEIELSKCIASITSLNNNKNFLQN
                     GLDASAKTIASLESQILTLTNENAQETGSHTATENLRNALNQFIDEQKQKQEQVNQLQ
                     TQSSEASQKYNQHNIQCIQQQNRIQTIKRDLEYKTNQQQSIETNQKNNAIELVNAIEQ
                     LAINQSTLTQLETELVELISEKQNFETALTVNETDYYKAKSEIDTEERAINDLRKQKE
                     NADTLIATN"
BASE COUNT          245 a          165 c          226 g          440 t
ORIGIN      
        1 ggttggttgc aattaatgta tcggcatttt ctttttgttt acgcaaatca ttaatagcgc
       61 gttcttcggt gtcaatttcg cttttggctt tgtagtagtc tgtttcgttt acggttaagg
      121 cagtttcgaa attttgcttt tcgcttatta attccactaa ctcagtttct aactgtgtta
      181 atgtactttg attaattgca agttgctcaa tagcattaac caattcaatg gcattgtttt
      241 tttgattggt ttcaatgctt tgctgttgat tggttttata ctctaaatcg cgtttaatgg
      301 tttgtatgcg gttttgctgt tgtatgcact gtatgttatg ctgattgtat ttttgcgatg
      361 cttcgctgct ttgagtttgt aattgattta cctgttcttg tttttgtttt tgttcgtcta
      421 taaattgatt taatgcatta cgtaaatttt cagtagctgt gtggctgcct gtttcttgtg
      481 cattttcgtt ggtaagggta agaatttggg actctaaact tgctatggtt ttggccgaag
      541 catctaagcc gttttgaagg aaatttttat tgttatttaa tgatgttatt gaggctatac
      601 atttactaag ttctatttcg gctgcgttaa ggcgctgacc ggtttctcgc agattggcat
      661 tggcagttgc aaaaagtttt tctgtggcta atatgtctgt ttgtatggtt tctacctgtg
      721 ctgcaagtgt tttaatagtt tcttcaagtg tttgcagatt tttaaattta ccggtgcgtt
      781 tgccatcaaa taaacctaca ctgccacctg aaacagtata atgttgttta agtaaagtac
      841 ctgaggtact cagtaatacc agtcggctat cggcctcaaa aatataatcg gtaacttgtt
      901 tattgtcggc cactaagtac acatggccca ataaatagtg cattaatggt tcgtatattt
      961 tatcaaattc aacaacgctg agtgcaggta tgctgtttgg tggtgctgtt tgtggtttat
     1021 gtacttgcat ttttaaaggt tcgagtacaa aaaaattggc acgtcctttt gatgat
//
LOCUS       DAOMAA010000974         1074 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_189559_length_1074_cov_1.836114, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000974 DAOMAA010000000
VERSION     DAOMAA010000974.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_189559_length_1074_cov_1.836114"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1074)
                     /locus_tag="PLO59_11185"
     CDS             complement(<1..>1074)
                     /locus_tag="PLO59_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01718.1"
                     /translation="ASVGSPCGGAAHICTAINVTAGNTYAIHLNYTNPSLNTFDMSFG
                     GTFQIAPYPDFYVDAPGGCFPHTTDFTDSSYAGTTYNWSFGNGNTFVGNNPPQQNYPL
                     AGNYVVGLVLSHAATGCTNATSRLIQVGAGPSSTFTTSQATICVGLNDTINYTGNGGP
                     LATFNWNFNGATIVSGSGSGPYIVQWNTSGTKTVTLTVTENGCQSNPTNVNVTVNDPP
                     TSTFSMPASGCVGDTIMIAYNGNAASTALYYWSLGNSFIAGGNNIDTFLVTWGAAGLD
                     SVYLIIDQLGCISNTTTQYITLGTKPISTFSLTDSICSSAQAIATYTGTSTGTAQYDW
                     DFGSGTAAPGGTVAGPQNIGFSAS"
BASE COUNT          314 a          222 c          242 g          296 t
ORIGIN      
        1 cgatgcggaa aagcctatgt tttgagggcc ggcaacagta ccaccagggg ctgctgtgcc
       61 acttccaaaa tcccagtcat attgtgcagt gccggtcgaa gtaccggtat aagttgcaat
      121 agcttgagcg ctgctgcata ttgaatcggt aagtgaaaat gttgaaatgg gctttgtgcc
      181 taatgtaata tattgagtag ttgtatttga aatgcatccc aattgatcaa taatcaaata
      241 aacgctgtct aatccggctg ctccccaggt tactaaaaac gtgtcaatat tattgccacc
      301 ggcaataaat gaattaccta aactccaata ataaagtgcc gttgaagctg cattgccatt
      361 gtaagcaatc attatggtat cgccaacaca acctgatgcc ggcatactaa atgtagaagt
      421 aggtgggtcg ttaaccgtta catttacgtt ggtgggatta gattgacaac cgttttcagt
      481 tacagtaagt gttacagttt tagtacctga agtattccat tgtacaatgt aagggccact
      541 accactgcca ctcactattg ttgcaccgtt aaaattccaa ttaaaagttg ctaatggtcc
      601 gccattgcct gtgtaattga ttgtatcgtt aagtccaacg catatcgtag cctgcgaagt
      661 tgtaaaagta cttgatggcc ctgcacctac ctgaattaaa cgagacgtag cgttggtaca
      721 acctgttgca gcatgcgaca gaaccaatcc cacaacgtaa ttgcctgcaa gcggatagtt
      781 ttgttgtggt ggattgttac caacaaaagt gtttccgttt ccaaaactcc aattataggt
      841 tgtgccggca taactcgaat ctgtaaaatc ggttgtatgc gggaaacaac ctccgggcgc
      901 atcaacataa aaatcggggt agggggcaat ttgaaaagtg ccgccaaagc tcatatcaaa
      961 agtgtttaaa cttgggttgg tgtagtttaa gtggatggcg tatgtattac cggcagttac
     1021 atttatagca gtacaaatat gtgctgcacc accgcatggc gaaccaacag aagc
//
LOCUS       DAOMAA010000975         1073 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_189794_length_1073_cov_2.343811, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000975 DAOMAA010000000
VERSION     DAOMAA010000975.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_189794_length_1073_cov_2.343811"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..>1073)
                     /locus_tag="PLO59_11190"
     CDS             complement(125..>1073)
                     /locus_tag="PLO59_11190"
                     /inference="COORDINATES: protein motif:HMM:NF018269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HQV01719.1"
                     /translation="QTKSIFGTVAEGQIHKSNVSNTFTYPDGETSTLHKPIYQIVQTY
                     KPLPPHVVVSARNAPPVFGMGLLEAISISAMQAVADETDADFNGISGKINWVWDIASA
                     SLMRGKFGYKASHTTALQQTAGAFNEDMGLTNSYFDAENAHGQSNAQIGLQTHIDVSD
                     ALLESAAFYVRTIAVPAARNTTNQAFVAGKQLFKSLNCNACHKEQWITGPAFIPELSH
                     QKIEPYTDLLLHDMGDDLADNRPEFSASGNEWRTAPLWGIGLTQVVNPKATFLHDGRA
                     HTIEEAILWHGGEAQKSKDDFVRLSKAEREKLLLFLKNL"
BASE COUNT          304 a          226 c          201 g          342 t
ORIGIN      
        1 tgaagttaaa tgtgcttatt tttttaaaat accctaatgc atcaaagcca caaaaatgta
       61 aaacgttgcc actttttaag tggccaataa aacaagcagc taagcaagaa aactttattt
      121 actgctaaag gttttttaaa aataacaata acttttcgcg ctcggcttta cttaatctta
      181 caaaatcatc tttcgatttt tgagcttcgc caccatgcca taaaatggcc tcttcaatgg
      241 tatgcgcacg gccgtcatgc aaaaatgttg ctttcggatt tacaacttgt gttaacccaa
      301 tgccccataa aggtgccgtg cgccactcat tgcctgaggc cgaaaattca ggtctgttgt
      361 cggctaaatc atcacccata tcgtgcagca ataaatcagt ataaggctca attttttgat
      421 gactgagttc gggaataaat gccggccctg ttatccattg ttctttatga catgcattgc
      481 aatttaaact tttaaataac tgtttgccag caacaaatgc ctgatttgtt gtattacgcg
      541 ctgccggtac tgcaattgtt cttacataaa aagcagcgct ttccaatagc gcatccgaaa
      601 catcaatgtg tgtttgtaaa ccaatttggg cattgctttg tccatgtgca ttttcagcat
      661 caaaatagct attagttaaa cccatgtctt cattaaaagc gcctgctgtt tgttgtaatg
      721 ccgtagtgtg cgatgctttg tagccaaact taccgcgcat taacgaagcc gatgcaatat
      781 cccataccca gtttattttg ccgcttatgc cattaaaatc cgcatctgtt tcatcggcaa
      841 cagcttgcat agcacttatc gaaattgctt ctagtaatcc cataccaaaa acaggtggtg
      901 cattgcgtgc cgatacaact acgtgagggg gcaaaggctt ataagtttgt actatttggt
      961 atattggttt gtgcagtgtg ctagtttcac cgtcaggata tgtaaaagtg tttgaaacat
     1021 ttgatttatg aatttgccct tcggcaacgg tgccaaaaat acttttggtt tgc
//
LOCUS       DAOMAA010000976         1072 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_190075_length_1072_cov_2.902655, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000976 DAOMAA010000000
VERSION     DAOMAA010000976.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_190075_length_1072_cov_2.902655"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="PLO59_11195"
     CDS             complement(<1..544)
                     /locus_tag="PLO59_11195"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682626.1"
                     /note="catalyzes the release of the N-terminal amino acid
                     from a tripeptide; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HQV01720.1"
                     /translation="MRNYTVSERFLKYVTIDTQADPDSITTPSSSKQFDLAKLLVNEL
                     QAMGITDAHVDEYCYVYATIPSNTHKNVPVVCFCSHMDTSPDCSGKNVNPVVHKSYEG
                     QTIILPAETSQQLNTNDHPELLNQIGNDIITADGTTLLGADNKAGIAEIMDAAYQLMN
                     DATIKHGTIKILFTPDEEIGR"
     gene            complement(563..>1072)
                     /locus_tag="PLO59_11200"
     CDS             complement(563..>1072)
                     /locus_tag="PLO59_11200"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQV01721.1"
                     /translation="LMGNKNLAGADLSKALSLGKDLAYVYYARAIIKKEMGDMQGSLV
                     EYNLALQYNENFEDALYNRAFLQNMLGSANDALADANKLIKLNPSNASNYNLRGNIHL
                     IFFRYEDAYNDYDKAISYDSNFAEAYYNRGLSLIMMNRLQMGCMDLDRSINLGYAPAQ
                     PVKQAFCKY"
BASE COUNT          293 a          200 c          196 g          383 t
ORIGIN      
        1 cacgcccaat ttcttcatca ggtgtaaata atatttttat agtgccatgt ttaatggttg
       61 catcattcat cagttggtag gctgcatcca taatttcggc aatgcctgct ttgttatcgg
      121 cccctaacaa tgtagtacca tcggcagtta ttatatcgtt tcctatttga tttaataatt
      181 caggatggtc gttagtattt aactgttgcg aagtttcagc aggcaaaata attgtttggc
      241 cttcgtatga tttatgtact acaggattca catttttacc actgcaatcg ggcgaagtgt
      301 ccatgtgcga gcaaaaacaa acaactggta catttttgtg ggtgtttgaa ggaatagttg
      361 catacacata acaatattca tccacatgtg catcggttat gcccatagct tgtaattcat
      421 ttactagcaa ttttgccaaa tcaaactgtt tgctcgatga gggtgttgta atggaatcgg
      481 ggtcggcttg ggtatcaatt gttacatatt ttaaaaaccg ttccgatact gtgtagtttc
      541 tcataataat ttttgtgcaa tattaatatt tacaaaatgc ttgttttacc ggttgcgctg
      601 gtgcataacc taaatttatg ctacgatcta aatccataca tcccatttgc aaacggttca
      661 tcataattag cgacaggcct ctgttgtaat atgcttcggc aaagttgcta tcatagctaa
      721 ttgctttatc atagtcattg taagcatctt catacctaaa aaaaatcaga tgaatgtttc
      781 cgcgcaggtt ataattgctt gcattgcttg ggtttagttt tattaatttg tttgcatcgg
      841 caagtgcatc gttggcacta cctaacatgt tttgtaagaa ggcacggttg tagagtgcat
      901 cttcaaaatt ttcattgtat tgtaatgcaa gattgtactc taccagtgaa ccctgcatat
      961 cgcccatttc ttttttaatt atagctcttg cataatatac ataagccaaa tctttgccta
     1021 aacttaatgc tttgcttaaa tctgctccgg ccaggttttt attacccata ag
//
LOCUS       DAOMAA010000977         1070 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_190692_length_1070_cov_3.714286, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000977 DAOMAA010000000
VERSION     DAOMAA010000977.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_190692_length_1070_cov_3.714286"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(203..841)
                     /locus_tag="PLO59_11205"
     CDS             complement(203..841)
                     /locus_tag="PLO59_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQV01722.1"
                     /translation="MEIKVAVFDDNKPRRELLQLLINSTDGLQCIHAFANCSNIVLNL
                     KGNLPDVILMDIDMPDVNGIEGLKLIRKHYPKIKVLMQTIFEDDEKIFDAIVAGADGY
                     ILKKAPPAKLLDAIVEVHEGGAPMTPTIARQVLQLFSNKQQRKEKTNFDLTTREQEIL
                     HYLVQGLSYKMIADKCNISYPTVNSHISHIYEKLQVNSGTEAVAKAINNKLF"
BASE COUNT          301 a          187 c          194 g          388 t
ORIGIN      
        1 cgtagaccga ccctatgata tcccctgcaa ttgcaccaat catgttttct ctccccaaaa
       61 gtgtacaaac aagacattaa ttaaaattaa agggctatgc attttttacg atgtttttaa
      121 attaacatca ggcaaagcta tccaatattt aaattcgttt acgttatggg cgtatttggt
      181 gttacaatag tgccgttatg tattaaaaca atttgttgtt aatagctttt gctacggctt
      241 cggtacctga gtttacctgt agtttttcgt aaatgtggct gatatgtgaa tttacggttg
      301 ggtaggaaat gttgcatttg tcggcaatca ttttataact aaggccctgt actaagtaat
      361 gcagtatttc ttgctcacgt gtggtgagat caaaatttgt tttttctttt cgctgttgtt
      421 tattactaaa aagttgtagc acctgtcttg caatggtagg tgtcataggt gcgccacctt
      481 catgcacttc aactatggcg tcaagcaatt ttgcgggtgg ggctttcttt aatatatatc
      541 catcggcacc agcaacaatg gcgtcaaata ttttttcgtc atcttcaaaa atggtttgca
      601 ttaaaacttt aattttcggg taatgtttgc ggataagttt caagccttca atgccattta
      661 catcgggcat atctatatcc attaaaataa catcaggcaa attgcctttt aagtttaata
      721 caatgttgct gcaattggca aaggcatgta tgcattgtaa accatcggtg ctgtttatta
      781 ataactgcag caattctctg cggggcttgt tgtcgtcaaa cacagctact ttaatttcca
      841 tggttcaaat ttgcagaaga taattattat taaaaatcat taaatgcaat gagataaaac
      901 tttgccggct ttaaataaac gagcattaag ttgtttttat aatacaaatt caattctaag
      961 ttcaacgccc agcataggcg cagttgttat atttaatttg cctttaagtt cggttactct
     1021 tttttttatg ttgacaaggc cattacctcc ggttttaaat ttttgttgca
//
LOCUS       DAOMAA010000978         1070 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_190788_length_1070_cov_2.532020, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000978 DAOMAA010000000
VERSION     DAOMAA010000978.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_190788_length_1070_cov_2.532020"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="PLO59_11210"
     CDS             <1..811
                     /locus_tag="PLO59_11210"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HQV01723.1"
                     /translation="QGQWSKPVSYTFKVLPPWYRSIGAYISYVILFASATWGFIRRRE
                     LKIKEKSDFEKRIAEVEMQALRSQMNPHFIFNSLHAINKYMLDNDKQNASAYLSHFSQ
                     LMRLILENSRQQEVPLEKDLSALELYMQLEQLRFQQKFTYAIEIDAMLNAETTLIPPM
                     LLQPFVENSILHGFKDKQNGFIQITINKTENNLHCIVQDNGIGRLNSINANASNTKKH
                     ESLAMKITQERLHIIEQSKNIKTNLSLIDMNAAGSDTGLRVELILPFEEAF"
     gene            838..>1070
                     /locus_tag="PLO59_11215"
     CDS             838..>1070
                     /locus_tag="PLO59_11215"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQV01724.1"
                     /translation="MIKAIIIEDEKHSQELLHEMIKTHIKHIEVMAICSNKAEGISAI
                     ISLQPDLVFSDIELGNDTAFDMLQQLPKIDFEV"
BASE COUNT          377 a          192 c          194 g          307 t
ORIGIN      
        1 ccaggggcaa tggagcaaac ccgtaagcta tacttttaaa gttttgccgc cttggtatcg
       61 tagtattggg gcatacataa gctatgtaat cctatttgca tctgcaacgt ggggttttat
      121 tcgcaggcgt gaattaaaga ttaaggaaaa atcggatttt gaaaaacgca ttgccgaagt
      181 tgagatgcag gcattacgaa gccagatgaa tccacatttt atcttcaact cattgcatgc
      241 catcaacaaa tacatgttgg ataacgacaa acaaaatgct tctgcttact tatcacactt
      301 ttcgcaattg atgcgattga ttttagaaaa ttcgcgacaa caggaagtac cactcgaaaa
      361 agatttatcg gctcttgaat tatatatgca attagagcaa ctacggtttc agcagaagtt
      421 tacttatgcc attgaaatag atgcaatgct taatgccgaa acaacactta ttcctccgat
      481 gctattgcaa ccctttgtcg aaaattcaat tttacatgga tttaaagaca aacaaaatgg
      541 cttcatacaa attaccatta ataaaaccga aaacaacctg cattgcattg ttcaggataa
      601 cggcattggc aggcttaact caataaatgc aaatgcatca aacacaaaaa aacatgagtc
      661 gcttgccatg aaaataacac aagaacggtt gcacattatt gaacaatcga aaaacataaa
      721 aacaaattta agtttgattg atatgaatgc tgccggaagc gataccggtt tgcgtgttga
      781 acttatatta ccttttgaag aagcatttta atggttgtct ttttttaact tcgtaccatg
      841 attaaagcaa ttataataga agacgaaaag catagtcagg agttgttaca cgaaatgatt
      901 aaaacccata ttaaacacat tgaagtaatg gctatttgtt cgaacaaagc tgaaggaatt
      961 tcggcaataa taagtttgca acccgatttg gtttttagtg atattgaact gggcaacgac
     1021 actgcatttg acatgttgca acaactaccc aaaatagatt tcgaagtaat
//
LOCUS       DAOMAA010000979         1070 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_190794_length_1070_cov_2.488670, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000979 DAOMAA010000000
VERSION     DAOMAA010000979.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_190794_length_1070_cov_2.488670"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="PLO59_11220"
     CDS             <1..816
                     /locus_tag="PLO59_11220"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014796184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HQV01725.1"
                     /translation="SIVQNGLQPVLIEPLLSTYNIDVSLIEAAITPKTKAIMPVHLYG
                     KMCQMDTIMEIANKYQLVVIEDAAQAHGASLKGIKAGAFGHMAGFSFYPTKNLGALAD
                     AGAVTTNSNVYNAALRRLRNYGSEVKYFNEVVGYNSRLDEMQAAFLQIKLKALDSINQ
                     HKKNLAKLYFENLKSDFILPVIEPDYDDVFHIFSIRHQQRDKLRSFLTQKNISTEIHY
                     PLPPNKQKAMNGIVDHCNTPVADLIHATTLSLPISYGHTQDDVLKVIETLNSF"
     gene            908..>1070
                     /locus_tag="PLO59_11225"
     CDS             908..>1070
                     /locus_tag="PLO59_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017324944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQV01726.1"
                     /translation="MDKSIVIEPGNEAANYWKDIWHFRSLFYFLAWRDVLVRYKQTTI
                     GIAWSIVRPL"
BASE COUNT          345 a          192 c          206 g          327 t
ORIGIN      
        1 tccatagtac aaaacggatt gcaaccggtt ttgatagagc cacttttatc tacgtataat
       61 attgatgtta gcttaataga agccgctatc acgcctaaaa ccaaagctat aatgcccgtg
      121 catttatatg gtaaaatgtg ccaaatggat acgataatgg aaattgcaaa caagtatcaa
      181 ctggtggtta ttgaagatgc agcgcaagca catggtgctt cgcttaaagg tattaaagca
      241 ggtgcatttg ggcacatggc tggttttagt ttttatccta caaaaaattt aggcgccctg
      301 gccgatgccg gagcagttac aaccaatagc aatgtttata atgctgcact tcgcaggcta
      361 cgcaattatg gctcagaagt aaagtacttt aatgaagtgg ttggttataa ctcgcgttta
      421 gacgaaatgc aagcagcatt tttgcagata aagcttaaag ctttggattc gataaatcaa
      481 cataaaaaaa atctggcaaa actttacttc gaaaatttaa agtctgattt tattttaccg
      541 gttatcgaac cggattatga cgatgtgttt catatttttt ctatcaggca tcagcaacgt
      601 gataagttaa gaagtttcct gacgcaaaaa aatattagca ccgaaattca ttacccattg
      661 ccacctaata agcaaaaagc catgaacggt attgtagatc attgtaatac acctgttgcc
      721 gatttgatac atgctactac attaagcttg cctatttctt atggccatac acaagatgat
      781 gtactgaaag tgattgaaac attaaattct ttttagttgt taatagcttt tataaaaact
      841 acagatagca accaaaacag ccatataaat tattgcaact ttgtgattcg tatttgtaca
      901 cctgtttatg gataaaagta ttgttattga accgggcaac gaagcagcca attattggaa
      961 agacatttgg cacttccgca gcttgtttta ttttttagcc tggcgcgatg tattggtgcg
     1021 atacaaacaa acaactattg gcatagcctg gagtatcgtg cgacccttgt
//
LOCUS       DAOMAA010000980         1068 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_191397_length_1068_cov_3.348470, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000980 DAOMAA010000000
VERSION     DAOMAA010000980.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_191397_length_1068_cov_3.348470"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1068)
                     /gene="dnaE"
                     /locus_tag="PLO59_11230"
     CDS             complement(<1..>1068)
                     /gene="dnaE"
                     /locus_tag="PLO59_11230"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HQV01727.1"
                     /translation="DGTGSLQEKYNLQSDDIENVKKIREFKAGNDLKAEVIREALVLE
                     GSVRGTGIHAAGIIIAPKDLTEIIPVSKSKETNLLITQFEGKIIENAGVIKMDFLGLK
                     TLTVIRDALKLIKQNHQINIDIDTIPLDDVKTLELYARGETNGTFQFESVGMQKYLKE
                     LKPDKFEDLIAMNALYRPGPIEYIPNFIARKHGREPVTFDLPDMEEYLAETYGITVYQ
                     EQVMLLSQKLAGFTKGDADNLRKAMGKKQIETLNKMKGTFLQGATAKGHPAEVLEKIW
                     TDWEAFASYAFNKSHSTCYALVAFQTAYLKAHYPSEYMAAVLTSNLGNIDKVTFYMEE
                     CRKMQMHVLGPDVNESNYMFS"
BASE COUNT          267 a          207 c          212 g          382 t
ORIGIN      
        1 cactaaacat gtagttgctt tcatttacat cgggccctaa tacatgcatt tgcattttgc
       61 ggcattcttc catgtaaaaa gttactttat caatattacc taagttactg gttaatacgg
      121 cagccatgta ctcgctggga taatgcgctt tgaggtaagc tgtttgaaaa gctactaagg
      181 catagcaggt tgagtgtgac ttgttaaatg catagctggc aaatgcttcc caatcggtcc
      241 atattttttc taaaacctcg gcaggatggc ctttggccgt ggcaccttgt aaaaacgtgc
      301 ccttcatttt attaagggtt tcgatttgtt ttttacccat agccttacgt aagttatcgg
      361 catcgccttt tgtaaagcct gctaattttt gtgatagtaa cattacctgc tcttggtaaa
      421 cggttatgcc ataagtttcg gcaaggtatt cttccatatc gggtaaatcg aaggtaacgg
      481 gttcgcggcc atgtttgcgg gcaataaaat tgggtatgta ttctattggg cccggcctgt
      541 aaagcgcatt catggcaata aggtcttcaa acttatcagg ttttagttct ttcaaatatt
      601 tttgcatgcc cacactttcg aattggaatg tgccattggt ttcgcctctg gcatatagtt
      661 ccaatgtctt aacatcgtca agtggtatgg tatcaatatc aatgtttatt tgatgatttt
      721 gttttataag ttttaaggca tcgcgtataa cggtaagcgt ttttaagcct aaaaaatcca
      781 ttttaatgac accggcattt tcaataattt ttccttcaaa ctgtgttatc agtaaattgg
      841 tttcttttga ttttgatacg ggtataattt cggttaaatc tttcggggcg atgataatac
      901 cggcagcatg tatgcctgta cctcttaccg aaccttctaa taccaaggct tcgcgtataa
      961 cttcagcttt taagtcgtta ccggctttaa actcgcgtat tttttttacg ttttcaatat
     1021 catcagattg caggttgtat ttttcctgaa ggctacctgt gccatcta
//
LOCUS       DAOMAA010000981         1068 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_191411_length_1068_cov_3.085884, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000981 DAOMAA010000000
VERSION     DAOMAA010000981.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_191411_length_1068_cov_3.085884"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="PLO59_11235"
     CDS             complement(<1..631)
                     /locus_tag="PLO59_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQV01728.1"
                     /translation="MTQDFLPLNGTDHIEFYVGNAKQASYYYQSAFGFKLTAYSGPET
                     GVRDRASYVLEQGKIKFVLTTAIHPKGEISEHVALHGDGVKVLALSVDDAEKSFYETM
                     SRGAKAHTEPTVISDEFGEVKIAAIHTYGDTIHKFIERKNYKGAFMPGYKPATSTIPV
                     ESVGLEVVDHCVGNVELGKMNEWVKFYEDVLGFKMIITFDDEDISTEYSA"
     gene            785..>1068
                     /locus_tag="PLO59_11240"
     CDS             785..>1068
                     /locus_tag="PLO59_11240"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HQV01729.1"
                     /translation="MTLLIVLVTILISFAAFNNPTLKSKLIFNAYLVAQRKQWYRMFS
                     YGFIHADWIHLAFNMLALYSFGNIVASYYTYYFGFTEGNLFFIFLYMSCL"
BASE COUNT          324 a          191 c          177 g          376 t
ORIGIN      
        1 atgctgaata ttcagtagaa atatcttcat catcaaaggt gataatcatt ttaaaaccta
       61 aaacatcttc ataaaacttt acccattcat tcattttacc taattcaaca tttcctacac
      121 aatggtcaac cacttctaag cctaccgatt caacaggtat ggtagaagta gccggtttat
      181 aaccgggcat aaaagcacct ttgtaatttt ttcgttcgat aaatttatgt atggtatcgc
      241 cataggtgtg tatggcagca atttttactt cgccaaattc atcactgata accgtaggtt
      301 ccgtatgtgc tttggcacca cgcgacatgg tttcataaaa agatttttcg gcatcatcca
      361 ctgaaagcgc aagcactttt acaccatcgc catgtagggc aacgtgttcg ctgatttcgc
      421 cttttggatg tatggctgta gttaaaacga atttaatttt tccttgttcc aaaacataag
      481 aagcgcgatc gcgcacaccg gtttcgggac ctgaatatgc tgtaagctta aacccaaaag
      541 ccgattggta ataataagag gcttgcttgg cattacccac ataaaattca atatgatcgg
      601 tgccatttaa aggaagaaaa tcttgtgtca ttgcttaata aatttgatta tttatagggg
      661 tgcaaatata ttttatttaa taaatattgc gcgctattac tgatacacta aacttaaata
      721 actgataata tttgaggcta cttaagccaa aattaaatta cgtttgctgt agtttaattg
      781 aattatgact ttactaattg ttttagttac catattgatt tcgtttgccg cttttaataa
      841 tccaacatta aaatcaaaac tcatatttaa tgcatactta gtagcccagc gaaagcagtg
      901 gtatcgtatg ttcagttatg ggtttataca tgccgactgg atacatttag cttttaacat
      961 gcttgcgctt tatagttttg gcaatattgt agcatcttat tatacttatt attttggttt
     1021 tacagagggc aacttatttt ttatatttct atacatgtca tgtttgtt
//
LOCUS       DAOMAA010000982         1068 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_191441_length_1068_cov_2.805528, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000982 DAOMAA010000000
VERSION     DAOMAA010000982.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_191441_length_1068_cov_2.805528"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1068)
                     /locus_tag="PLO59_11245"
     CDS             complement(<1..>1068)
                     /locus_tag="PLO59_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009411031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQV01730.1"
                     /translation="MQLFNVSANLMSLGAIDFGLIVDGSVIIVEATMHHLGLRKSTVK
                     LTQSEMDGEVFKSASAIRNSAAFGEIIILIVYLPILTLVGIEGKMFRPMAQTVGFAII
                     GALILSLTYIPMMCAWLLPKTTVTNTSFSSRMMQRFERIYATLLEKVIRIKKTVIAIS
                     LALFLISIIAFLKLGGEFIPTLQEGDFAFHCILPQGTSLSQSLETSMQVSRIIKEFDE
                     VKMVVGKTGSAEVPTDPMPPEATDIMVILKHPDQWKRNISYDALADEIAAKLTVVPGV
                     FFEKSQPIQMRFNELMTGIRQDIAVKIFGEQMDTLLLYANKVAAEIQNVKGTTAPNVE
                     RVEGLPQISVKYNRAQIASYGL"
BASE COUNT          322 a          221 c          191 g          334 t
ORIGIN      
        1 caaaccatag cttgcaattt gggctctatt gtatttgaca ctaatttgtg gcaagccttc
       61 aacacgctct acattgggtg ctgttgtacc ttttacattc tgaatttcgg cagcaacttt
      121 gttggcatat aataataatg tatccatttg ctcaccaaaa attttaacgg caatatcttg
      181 tctgatgcct gtcatcagtt cattaaatcg catctgaatg ggttgacttt tttcgaaaaa
      241 cacacccgga acaactgtaa gtttagctgc aatttcatcg gccaaggcat cgtaagatat
      301 attacgtttc cattggtcag gatgttttaa aattaccatg atatcggtag cttcgggtgg
      361 cattgggtcg gtgggtactt cggcacttcc ggttttacct actaccattt ttacctcatc
      421 aaattcttta attatacgcg aaacctgcat gctggtttct aaactttgtg atagagatgt
      481 gccttggggt aaaatacaat gaaaagcaaa atcaccttct tgtaatgtgg gtataaactc
      541 gccaccaagt tttaaaaaag caataatcga aattaaaaat aaggcaaggc ttattgcaat
      601 aactgttttt ttaattcgaa taactttttc gagtaacgtg gcatatatac gttcgaagcg
      661 ctgcatcatc ctgctgctaa aagatgtatt ggtaaccgta gtttttggca ataaccaagc
      721 gcacatcatg ggtatgtagg ttaatgataa aattaaagca cctattatgg caaagcctac
      781 cgtttgtgcc ataggcctga acatcttgcc ttcaataccc accaacgtaa gtataggcag
      841 ataaacaatt aagataataa tctcgccaaa tgctgcactg tttctgatag ccgatgccga
      901 tttaaaaacc tctccatcca tttcgctctg tgtaagtttt actgttgatt tacgtaaacc
      961 caaatgatgc atggttgctt caacaataat taccgagcca tcaactatca acccaaaatc
     1021 aatggcacct aaactcatca ggttggcgct tacattaaac aactgcat
//
LOCUS       DAOMAA010000983         1068 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_191493_length_1068_cov_2.433366, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000983 DAOMAA010000000
VERSION     DAOMAA010000983.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_191493_length_1068_cov_2.433366"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="PLO59_11250"
     CDS             <1..619
                     /locus_tag="PLO59_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015029218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HQV01731.1"
                     /translation="KAMNRPLSNVIFGAFGGGGATVGGPDVKGSIKDIQVSDLAVQMN
                     YARKVVIVPGYGLAVAQAQHVIHELEMLLEERGVEVKYAIHPVAGRMPGHMNVLLAEA
                     NVSYDKLVEMDDINPEFEQTDVVLIVGANDVVNPAAKSDPSSPIYGMPILEVDRAKSV
                     VVNKRSMNAGYAGIDNLLFYDPKCSMFFGDAKKALTGLVAELKTM"
     gene            757..>1068
                     /locus_tag="PLO59_11255"
     CDS             757..>1068
                     /locus_tag="PLO59_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010179992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family peptidase"
                     /protein_id="HQV01732.1"
                     /translation="MAKKSTSILTKNSLTFLEKYINNAAPTGFESEGQKLWLNYIRPY
                     IDEHIVDTYGTVVGVINPKSDYKVVIEAHADEISWFVNYITNDGFIYLKRNGGSDHQI
                     AP"
BASE COUNT          332 a          189 c          227 g          320 t
ORIGIN      
        1 taaagcaatg aatcgccctt tgagcaatgt aatttttggt gcctttggtg gcggcggtgc
       61 tactgtcggt ggcccggatg taaaaggttc aataaaagat attcaggtga gcgatttggc
      121 tgtacaaatg aattatgctc gaaaagtggt aattgttccg ggatatggat tggccgtagc
      181 acaagcacaa cacgttattc atgaattaga aatgctgctc gaagagcgtg gcgttgaagt
      241 taaatatgcc attcacccgg ttgcaggtcg tatgcccgga cacatgaacg ttttgcttgc
      301 cgaagcaaat gtttcctacg ataaattagt agagatggat gacattaatc ctgaatttga
      361 acaaacagat gtggtgttga ttgtgggtgc aaacgatgtg gttaaccctg ctgcaaaaag
      421 cgacccttcc tctcctattt atggaatgcc catcttagaa gtggatagag caaaaagtgt
      481 agttgtaaac aaacgttcta tgaatgcagg ttatgccggt atcgacaatt tattgtttta
      541 tgatcctaaa tgcagtatgt tttttggtga tgctaaaaaa gccttaacgg gactggtagc
      601 cgagttaaaa acaatgtaat ttttaataaa tcgaatttcg aaagggctgc tggttaaaca
      661 aagcagcctt ttctttttca aaaattgtaa gatatttctt gacaatgggt aaaaattatt
      721 tttatttcaa cctttaattc ttcagatata taacccatgg ctaaaaaaag cacttcaata
      781 cttacaaaaa actctcttac ttttttagaa aaatacatca ataacgctgc acctacaggc
      841 ttcgaaagcg agggtcaaaa actttggctc aattatattc ggccctatat cgatgaacat
      901 atagtagata cctatggcac tgtggttggg gttatcaatc caaaatcgga ttataaagtg
      961 gtgattgaag ctcatgccga tgaaatttcc tggtttgtaa attacatcac caacgatggc
     1021 tttatttacc taaaacgcaa tggcggttca gaccatcaaa tcgcgcca
//
LOCUS       DAOMAA010000984         1068 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_191499_length_1068_cov_2.417572, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000984 DAOMAA010000000
VERSION     DAOMAA010000984.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_191499_length_1068_cov_2.417572"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1068)
                     /locus_tag="PLO59_11260"
     CDS             complement(<1..>1068)
                     /locus_tag="PLO59_11260"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HQV01733.1"
                     /translation="PGSGSRIRIRGGTSLNASNDPLIVVDGVPLDNNGISGAPNGLSL
                     INPNDIENMTVLKDASAAAIYGARAANGVIIITTKKGSASSFHVDFNTNFAISTVADY
                     TDVLSADEFRNVVMANGTNTQKGLLGKSVTNWQSEIYRNALTSDNNISFTGGIKGLPY
                     RLSFGLLSNAGTLQYSQMDRKSVGINLNPSFFDNSLTLEVNAKYSTTYNFFPDQGAIG
                     SAVGFDPTQNIYSDTTAYGGYFEWLDNSKKPIVVAARNPMGLLNLRDDKGFVNRFIGN
                     AGLDYKFKFLPGLRAHVNVGGDISRSHGSTFTSAAAASAYTTKGSQTQYEQSKDNKLF
                     DFYLNYNKEFNPNNRLDLTAGY"
BASE COUNT          311 a          226 c          204 g          327 t
ORIGIN      
        1 gtatcctgct gttaaatcca atcggttatt gggattaaac tctttattgt aattcaaata
       61 gaagtcaaac agtttattgt ctttgctttg ctcatattgt gtttggctac ccttagttgt
      121 ataggccgat gcggccgcgg ccgatgtaaa ggtactaccg tgactgcggc taatatcacc
      181 acccacatta acatgagcgc gtaagcctgg taaaaatttg aatttgtagt caagcccggc
      241 attaccaata aaacgattta caaatccttt atcatcacgt aagtttaata gccccattgg
      301 gtttcgggct gctacaacaa tgggtttttt agaattatcc aaccattcaa aatagccgcc
      361 ataagcagtt gtatcactgt aaatattttg agtaggatca aaacctactg ccgatccaat
      421 tgcgccttgg tcgggaaaga aattataagt ggtactgtat tttgcattca cttctaatgt
      481 taaactatta tcgaaaaatg aagggtttaa atttatgccc accgatttac gatccatttg
      541 cgagtactgc agggtgccgg cattgcttaa taagccaaac gataagcgat atggtaatcc
      601 ttttatgcct ccggtaaaac tgatattatt atcgctggtt agcgcgttac ggtaaatttc
      661 gctttgccag ttggttaccg atttgcctaa aagccctttt tgcgtattgg taccatttgc
      721 catcaccaca tttctaaact catcagcact aagcacatcg gtataatcgg caacggttga
      781 aatggcaaaa ttggtgttaa agtcaacatg gaacgatgaa gcggaacctt ttttagtagt
      841 tatgataatc acaccattag ctgctcttgc cccataaata gccgcagctg acgcgtcttt
      901 taacacagtc atattttcaa tgtcgttagg gttaattaaa cttaaaccat taggcgcacc
      961 actaatgcca ttgttatcca atggtacacc atcaacaaca attaaaggat cattgcttgc
     1021 atttaatgat gtaccacctc gaatacgaat gcggcttccc gaaccagg
//
LOCUS       DAOMAA010000985         1068 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_191510_length_1068_cov_2.344521, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000985 DAOMAA010000000
VERSION     DAOMAA010000985.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_191510_length_1068_cov_2.344521"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(540..>1068)
                     /locus_tag="PLO59_11265"
     CDS             complement(540..>1068)
                     /locus_tag="PLO59_11265"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV01734.1"
                     /translation="TTNAQPKLQVVLDQLPDDYDLKLYNSAGTQIANSQNGGTTAETI
                     NYNGASSGSTYTLQVYGYNGVFNAACYRLRVNTSATNFRITESAISNKANFSMYPNPA
                     NALVTINLFSDESQDATINLIDITGRVVYTQTISIAEGDQKMELDINQITKGIYFLQL
                     NANGDKTVQKLVIEK"
BASE COUNT          296 a          158 c          207 g          407 t
ORIGIN      
        1 aatcatggcc taatcgtaag gcttcttcgc ggctgtaggc gattacatct tttactcttg
       61 gtgaaaattt agcttccata aatcaggtat ttaactttgg taatgtatat atttagtcga
      121 acgattatgc tgtgcgtaag tatttatgtt tttaatgcca ttgtaaaact atgggttgtg
      181 tattagagtt tgctaaccta agctgaattt tacttgatgc atacgacctt tattattttt
      241 tttaaaatta attcacgctc aattgttcat tttttggaat gatgtatcaa ctaatgctat
      301 gccatgttta attctgatct gttatgactt gttttttcaa agaaactatt gaaaggctga
      361 cttttaatta gtcggcaata tgtttaaatg ccaattttgc agtaagttta tttataaatg
      421 aattgcatgt attcgtttgg cctctattgt tatataccta tttgaagtga tattggtttg
      481 ataagtgcaa aaaaaagccc tgaatatgat tcagggcttt tttaatgagg gtggattaat
      541 tatttttcaa taaccaattt ttgaacggtt ttatcgccat ttgcatttaa ctgtaggaag
      601 taaatgcctt tagttatttg attgatgtct aactccattt tctgatcgcc ttcggcaata
      661 gagatggttt gtgtataaac tacgcgaccg gttatgtcaa ttaaatttat agttgcatcc
      721 tgcgattcat cgctaaataa attgatggtt acaagtgcgt tagccggatt tggatacatg
      781 ctaaagttag ctttgttgct tatagccgat tcggtaatac ggaagttagt tgcactggta
      841 tttacacgta aacggtagca agctgcatta aatacaccat tgtaaccata aacttgtaag
      901 gtataggtac tgccacttga cgcgccattg taattaatgg tttcggcagt agttcctccg
      961 ttttgtgagt tagcaatttg tgtaccggca ctgttgtata attttaaatc ataatcatca
     1021 ggtagctgat caagtacaac ttgtaactta ggttgtgcat tggtggtt
//
LOCUS       DAOMAA010000986         1067 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_191779_length_1067_cov_3.083004, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000986 DAOMAA010000000
VERSION     DAOMAA010000986.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_191779_length_1067_cov_3.083004"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="PLO59_11270"
     CDS             <1..425
                     /locus_tag="PLO59_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008240935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HQV01735.1"
                     /translation="FNVRVYGLLIHQQNLLLADESFAGKTFTKFPGGGLQFGESTVDC
                     LKREFMEEYGWDVIVKQHFYTTDFFVPSVFDARQQVLAVYYFVEVNETLLSFNSLHAL
                     DGSGSLRWKSLNTLVIADVTFPIEKHVVHLLLQLNNRS"
     gene            complement(444..>1067)
                     /locus_tag="PLO59_11275"
     CDS             complement(444..>1067)
                     /locus_tag="PLO59_11275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HQV01736.1"
                     /translation="IYLNKSVTGKASFYGAKFEGRKTANGEIFSNKNLTAAHRTLPLN
                     SIVRVTVKENSRYIFVRINDRGPFVKGRIIDLTDAGAKGLDFVHAGTELVNVRLLEEI
                     QLTPELKQRFLSTEYLHDCLGNTTTLKQKALSIGTFLSIEHALYNACDLYNDNRLNSI
                     AIKAIKVGKHLRYLVIIHDIPLTADLELLVRIAQSRGFTNAKAYVYN"
BASE COUNT          304 a          182 c          209 g          372 t
ORIGIN      
        1 tgtttaatgt gcgtgtttat gggctgttaa ttcatcaaca aaatttattg ttggctgacg
       61 aaagctttgc aggtaaaacc tttactaaat ttccgggtgg tggcttgcaa tttggcgaaa
      121 gtacggttga ttgtttgaag cgcgagttta tggaagagta tggttgggat gttattgtaa
      181 aacaacattt ttacaccact gatttttttg tgccttcggt atttgacgca aggcaacaag
      241 tacttgcagt ttattatttt gttgaagtta atgagacttt gcttagtttt aattcattgc
      301 atgcattaga cggtagtggt agcttgcgtt ggaaatcatt aaacacttta gttattgccg
      361 atgttacttt tcctattgaa aagcatgttg tacatctttt gttgcaattg aataaccggt
      421 cttaaaacaa actaaatttt ttatcaatta taaacgtatg cctttgcatt ggtaaagccg
      481 cgcgactgtg caatacgaac caataattca aggtcggccg ttaaaggtat gtcatgtata
      541 attaccaaat agcgtagatg cttaccaact ttaatagcct taatggctat agagtttaaa
      601 cggttgtcat tatataaatc gcaggcattg tacaatgcat gttcaattga taaaaaagta
      661 ccaatagaaa gcgctttttg ttttagcgtt gttgtgttac ctaaacaatc gtgcaaatac
      721 tcagtactta aaaaacgttg tttaagttcc ggggtaagtt gtatctcttc taatagcctt
      781 acattaacta attcagtacc ggcatgcaca aagtctaaac ctttggcacc tgcatcagtt
      841 aggtcaatta tccgtccttt aacaaaaggg cctctgtcat ttatgcgcac aaaaatgtag
      901 cggctatttt cttttaccgt aacgcgcaca atactattta gtggtagggt tcgatgcgct
      961 gctgttaaat ttttattcga aaatatttca ccatttgctg tctttcggcc ttcaaatttg
     1021 gctccataaa acgaagcttt gcccgtaacc gatttgttca ggtaaat
//
LOCUS       DAOMAA010000987         1063 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_193230_length_1063_cov_2.571429, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000987 DAOMAA010000000
VERSION     DAOMAA010000987.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_193230_length_1063_cov_2.571429"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..611)
                     /locus_tag="PLO59_11280"
     CDS             complement(48..611)
                     /locus_tag="PLO59_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017259369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HQV01737.1"
                     /translation="MQNLFIQDTTIKNETALQKGEYENCTFINCNFESNDISNFIFID
                     CTFNSCNLSLAKLNNTAFRVVKFIDCKMLGLKFETCNDFGLSFSFDACQLNHSSFYKT
                     KIKRAIFKNTILQEVDFTECDLTEGFFDNCNMLHAVFDETILNKVNFTTAYNYIIDPD
                     INQIKKAKFSQSGIVGLLNKYDIVITD"
     gene            794..>1063
                     /locus_tag="PLO59_11285"
     CDS             794..>1063
                     /locus_tag="PLO59_11285"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HQV01738.1"
                     /translation="MGKLIVNRLDYARIKKCIKDAKQFKSITATEAAKLVNELDSAKI
                     VEPKAIPADVVTMNSIVKLSFLNTNKKIQFQIVYPDKAEIKENKIS"
BASE COUNT          383 a          139 c          162 g          379 t
ORIGIN      
        1 ataaataatt aagtatttaa atggagctat gtttaaatgg aatgtaatta atctgttata
       61 acaatatcgt acttatttaa taaccccacg attccggatt gtgaaaattt tgctttttta
      121 atttgattga tatccgggtc aatgatgtaa ttgtaagctg tagtaaaatt tactttgttg
      181 agtatggttt catcaaacac agcatgcaac atattacagt tgtcgaaaaa accttcagtt
      241 aaatcacatt cagtaaaatc tacttcttgt aatattgtat ttttaaaaat agctctttta
      301 atttttgttt tataaaatga actatgattt aactggcaag catcaaatga aaaagaaagg
      361 ccgaagtcat tacaagtttc aaacttaagc cccaacattt tgcaatcaat aaattttact
      421 accctaaaag ccgtattgtt taatttggct aagcttaggt tacaactatt aaaagtacag
      481 tcaatgaaaa taaagttcga aatatcattg ctttcaaaat tgcaatttat aaatgtgcag
      541 ttttcatatt cgcctttttg taaagcagtt tcgtttttaa tagtagtatc ctgaataaat
      601 aaattttgca taaggtattt gtaaattatt gatacaaagt aattgttatt tagatgcctt
      661 tggctaagtt tattatatac acaaagcaac atatttatgg gtttattttt aagttaagct
      721 tgcattgttg ttcaatatat gcctttttta ctttcttata tttggcagat aataaattat
      781 aaattgtatt aatatgggaa aattgatagt taaccgattg gattatgcac gcataaagaa
      841 gtgcattaag gatgccaaac aatttaaatc aattacagca acagaggctg caaaattggt
      901 taatgagcta gattcggcca aaatagtcga gcccaaagcc ataccggctg atgtggttac
      961 aatgaattca attgtgaagt tgagtttcct gaatacgaat aaaaaaattc agtttcaaat
     1021 tgtttaccct gataaggctg aaataaaaga aaataaaatt tca
//
LOCUS       DAOMAA010000988         1060 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_194331_length_1060_cov_2.276617, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000988 DAOMAA010000000
VERSION     DAOMAA010000988.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_194331_length_1060_cov_2.276617"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1060
                     /locus_tag="PLO59_11290"
     CDS             <1..>1060
                     /locus_tag="PLO59_11290"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HQV01739.1"
                     /translation="RVLLNKHNIKAELTATTRTGMHRYTFPKTKQATIVIDLKHRDEV
                     LDSYLTVVNDSTIKGMRRSKAWATDQYVYFYIRFSKPFKQYTVALNDAAIRSNTAQGK
                     NVKAHVDFNLPQGGIVLVKIGISAVSENGAQQNLDAENSSWDFDATAKQTRQYWNKEL
                     AKIEVKGKATDEAIFYTALYHCMLNPNTYNDVDGQYRGTDLKIHLMPGNMKNYYTVFS
                     LWDTFRGLHPLLSIIDEQRTNDFINTFLLQYEQGGLLPVWEFSGNETYCMIGYHSVPV
                     IVDAYAKGIKGFDAAKALKAMVHSASTDKYGLPCYRKFGFLANDCEHESVSKTLEYAY
                     DDWCIAQFAQLTGDATTY"
BASE COUNT          358 a          211 c          207 g          284 t
ORIGIN      
        1 accgcgtgct gttaaataaa cacaacatta aagccgaact tacagccact acacgtacag
       61 gcatgcatcg ctatacattt cctaaaacca aacaagcaac cattgtaatt gatttaaaac
      121 atcgtgacga agtacttgac tcatatctta ctgttgtaaa cgattcgacc ataaaaggca
      181 tgcgcagaag caaagcctgg gctaccgatc agtatgtata tttctacatc cgtttttcca
      241 aaccatttaa acaatacacg gttgccttaa acgatgcagc catacgcagc aatacagcac
      301 aaggtaaaaa cgtaaaagca catgttgatt ttaacttacc acaaggaggt atcgtgctgg
      361 ttaaaatagg cattagcgct gtaagcgaaa acggtgcaca gcaaaactta gatgctgaaa
      421 acagtagttg ggattttgat gcaacagcca aacaaacacg tcaatattgg aataaagaat
      481 tagctaaaat tgaagtaaaa ggtaaagcca ctgacgaagc cattttttac actgctttat
      541 atcattgcat gcttaatcca aacacatata acgatgtgga tggtcaatac cgtggcaccg
      601 atttaaaaat acacctgatg ccgggtaata tgaaaaacta ctacacggtg ttttcattgt
      661 gggatacttt cagagggctg catcccctac tcagcatcat tgacgaacaa cgcaccaacg
      721 attttataaa tacattttta ttacaatatg agcaaggtgg cttgttgccc gtatgggaat
      781 tttcaggcaa tgaaacctat tgtatgattg gctatcacag cgtgcctgta atagttgatg
      841 cttatgcaaa aggcatcaaa ggctttgatg ctgccaaagc attaaaagca atggtgcata
      901 gtgcttctac tgataaatat ggcttgccat gttatcgtaa atttggattt ctggcaaacg
      961 attgcgaaca cgaatcggta agtaaaactt tagaatatgc ttatgacgat tggtgcattg
     1021 cgcaatttgc gcaattaacc ggtgatgcaa ctacgtatga
//
LOCUS       DAOMAA010000989         1060 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_194434_length_1060_cov_1.806965, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000989 DAOMAA010000000
VERSION     DAOMAA010000989.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_194434_length_1060_cov_1.806965"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1060
                     /locus_tag="PLO59_11295"
     CDS             <1..>1060
                     /locus_tag="PLO59_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01740.1"
                     /translation="YACGINHVFTVINPLSNLNYTWHTNTGIAGFGTTAVYDFIANGY
                     TGGTLTFTATDPATGCQAASLPFIVAPCCTGDYTFTDVSASWVYANYPAIFYINSGTP
                     CVSLNTGTIAINGTFTIDMDMCIDHTQILLGPDAHIDIIPGIAVNTTGYTHLHTCNGN
                     YMWQGITVNTDAALSIDHTLVEEAQQAIICMPNATVNTASASIFNANYKGIVFNLNGA
                     AHTGVLQKTVFECNHQSNGTALTLIPPFAGQNPACGIEINTAAAIKIGNESNSSLFPP
                     SHQNLFRNLNLGIDLQNSQAEIVNNKFENIRRYQSNVFLSNGYCIKGANPNGLITEIT
                     VGGSNILPLKIYRSNEFEN"
BASE COUNT          321 a          250 c          206 g          283 t
ORIGIN      
        1 tatgcctgtg gcattaatca tgtttttact gtaatcaatc cattatctaa tttaaactat
       61 acgtggcata ccaatacggg tattgccggt tttggcacta cggcagttta cgattttatt
      121 gccaatggct acacaggggg caccctaaca tttaccgcca ccgaccctgc tacgggctgc
      181 caggcagctt cattgccatt tattgttgca ccctgctgca ccggtgatta tacgtttaca
      241 gatgtatcgg catcgtgggt atatgctaac tacccggcca tattttatat caattcgggt
      301 acaccctgtg tatcgttaaa cacaggcacc attgccataa acggcacctt taccattgat
      361 atggatatgt gcattgacca tacgcaaatt ttactgggcc ccgatgccca tattgatata
      421 atacccggca tagccgtaaa tactacaggc tacacccacc tgcacacctg taacggcaac
      481 tatatgtggc agggcattac cgtaaatacc gatgccgctt taagtataga tcatacactt
      541 gttgaagaag cgcaacaggc cattatatgt atgcccaatg ccacggttaa taccgcatcg
      601 gccagtattt ttaatgccaa ttataaaggc attgtgttta atttaaatgg ggcagcacat
      661 acgggcgttt tgcaaaaaac agtgtttgaa tgcaaccacc aaagcaatgg tactgccctt
      721 accttaatac ccccatttgc agggcaaaac cccgcctgcg gtatagaaat taatactgct
      781 gctgcaatta aaattggtaa cgaaagtaac agcagcctgt ttccgccaag ccaccaaaac
      841 ctgttccgaa acttaaacct gggcattgat ttacaaaaca gccaggccga aatagtaaac
      901 aacaagtttg aaaatataag gcggtaccaa agcaatgtgt ttttatccaa tggctattgc
      961 ataaaaggcg ccaaccccaa tggcctaatt actgaaatta ccgtaggtgg cagcaatata
     1021 ttgccattaa aaatttacag aagcaatgaa tttgaaaatg
//
LOCUS       DAOMAA010000990         1055 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_195998_length_1055_cov_3.133000, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000990 DAOMAA010000000
VERSION     DAOMAA010000990.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_195998_length_1055_cov_3.133000"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="PLO59_11300"
     CDS             complement(<1..271)
                     /locus_tag="PLO59_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011586628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01741.1"
                     /translation="MPKNQSIKSVLIIGSGPIVIGQACEFDYSGSQASRSLKEEGIEV
                     TLINSNPATIMTDKVTADNIYLKPLTKKSIVEILEKHKIDAVLPTM"
     gene            complement(534..692)
                     /gene="rpmH"
                     /locus_tag="PLO59_11305"
     CDS             complement(534..692)
                     /gene="rpmH"
                     /locus_tag="PLO59_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HQV01742.1"
                     /translation="MKRTYQPSNRKRSNKHGFRKRMSSANGRRVLAARRARGRRVLTV
                     SDERTYIK"
BASE COUNT          294 a          181 c          213 g          367 t
ORIGIN      
        1 ccatggtggg tagtactgca tctattttat gtttctctaa aatttcgaca atcgactttt
       61 tggttagggg cttaaggtaa atgttgtcgg ccgttacctt atcggtcatt atggtggccg
      121 ggttcgaatt aattagcgta acctcaatac cttcttcttt aagcgagcgc gatgcttgcg
      181 agcctgaata gtcaaattcg caggcttggc caattacaat aggccctgag ccaataataa
      241 gaaccgattt tatactttgg tttttaggca tatatgtttt agttaatttc tttgagattt
      301 aattatgcgc tacccatgct tacccctttt aacagcagtg ttaacagccg ttttaacaag
      361 gcttcaaaat tagaatttta tatgggctgc tttaaatttg catttaaatt acttgtacac
      421 aacctgtgta aaacctaacc tgttgcttgg ttttaagcca caaaaaaagg ccgctgctaa
      481 gaatcagcga cctttaggtg aagtttatac gtaactaaat cttcctgaag ggattatttg
      541 atgtaagtgc gttcatcact tacggtaagc actctgcggc cacgtgctct gcgtgctgct
      601 aataccctac ggccattagc tgacgacatt cttttacgaa aaccatgctt gttgctgcgc
      661 ttacgattac tgggttggta cgtccttttc attttatctg attttttgag agccaacgga
      721 cctaaccgcg acttttaatt taactgaggc aaaggtaata ttttaccgtg agggagaaag
      781 gtttttatcg aaagtttttg attttttttt tgcacactaa aaagttatca aatttttaga
      841 gtgctaattt gagttaggta aaatggctga ataatgcata cgtaatacat tgattattat
      901 ttgtttgcgt gtagaaaatt ggttaaagaa acagtaaaaa ttacatgggc agataaacta
      961 taaatttggt ttcgaaaaaa ttttggtcat aatactgact taatggcacc aaggttattt
     1021 tctttttaaa gggttgtagt tcagcggtta ggtcg
//
LOCUS       DAOMAA010000991         1052 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_197316_length_1052_cov_2.003009, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000991 DAOMAA010000000
VERSION     DAOMAA010000991.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_197316_length_1052_cov_2.003009"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1052)
                     /locus_tag="PLO59_11310"
     CDS             complement(<1..>1052)
                     /locus_tag="PLO59_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01743.1"
                     /translation="SQIHNADNQGFAINTKLNNKYALQKNKADSAWVLHTTLAVEHLH
                     KNFRFIEPYRPIEFARDWNLATTNATGAETATTMLFAAMQKQQLISYQLKTFFKGSYN
                     GLQHQLNMQTQMGKWHNKGYVTYLHSKANGANSGYIRAFSNQSYALKRVTVGVQYETE
                     NNKLFNAADTLTAQSFFYQQYDAYITNAAAANRFTYKISGGQRYDFAIDKAHFKYSNH
                     ADNMAADITLNGKTNGRFMAGFIYRQLHGADSIIQIAGSQTLLQKMGYTNQWLNGGIN
                     LNTYYEASTGQELRKEFSYLEVQPGTGVYTWNDYNLNGVKELNEFEVSAFTDQANYIR
                     IFVPTTTYIRTISTQF"
BASE COUNT          263 a          203 c          225 g          361 t
ORIGIN      
        1 ttaaattgtg tgcttatggt tcgtatgtaa gtagtagttg gcacaaaaat gcgtatgtag
       61 ttggcctgat cggtaaaggc cgaaacctcg aactcattaa gctctttaac tccgtttaag
      121 ttgtaatcat tccatgtata aactccggtg ccgggttgta cctcgaggta ggaaaactcc
      181 ttacgtaact cctgacccgt acttgcctca taatacgtat ttaggtttat acccccatta
      241 agccattgat tggtatatcc cattttttgt aacaatgttt ggctgccggc aatttgtata
      301 atactatcgg caccatgcaa ttgccggtaa ataaaaccgg ccataaaccg gccattggtt
      361 ttgccattga gggttatatc agccgccatg ttatcggcat gatttgaata tttaaaatgt
      421 gctttgtcaa ttgcaaaatc gtaccgctgg ccaccactta ttttgtaggt aaaacggtta
      481 gctgctgctg cattggttat ataagcatcg tactgctggt aaaaaaaact ttgtgctgtt
      541 aaggtatcgg ctgcattaaa taatttattg ttttcagttt catattgcac accaactgta
      601 acacgtttta acgcataact ttgattacta aatgcgcgta tatagccgct gttagctccg
      661 tttgctttac tgtgcaggta tgttacataa cctttgttat gccacttgcc catttgcgtt
      721 tgcatgttaa gctgatgttg caatccattg taagagcctt taaaaaatgt ttttagctga
      781 tagcttataa gttgttgctt ttgcatggct gcaaacagca tggtggtggc ggtttcggca
      841 ccggtggcat tcgtagttgc caggttccag tcgcgcgcaa attcaatagg cctgtatggt
      901 tcaataaatc tgaaattttt atgcaggtgc tcaacagcta atgtggtgtg taacacccag
      961 gcactatcgg ctttgttttt ttgcaaagcg tatttgttat ttaatttagt gtttatggca
     1021 aagccctggt tatcggcatt gtgtatttgg ct
//
LOCUS       DAOMAA010000992         1051 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_197654_length_1051_cov_2.289157, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000992 DAOMAA010000000
VERSION     DAOMAA010000992.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_197654_length_1051_cov_2.289157"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="PLO59_11315"
     CDS             complement(<1..334)
                     /locus_tag="PLO59_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HQV01744.1"
                     /translation="MESNKVRLRFAPSPTGPLHMGGVRTALYSYLFAKQHGGDFILRI
                     EDTDQNRFVPGAEQYIIDALNWCGIIPNEGVGFGDGKHAPYRQSERKPLYKQYAEQLI
                     GSGHAYYAF"
     gene            575..901
                     /locus_tag="PLO59_11320"
     CDS             575..901
                     /locus_tag="PLO59_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01745.1"
                     /translation="MYNITRVDSLNNFFGFEQTLLNPKNNNKVDLSMNYKPGYEAPLL
                     LTEMDSSGIILKSKKIKFFSDSLQFFCNGARWPSGTQFALAKSLYSPIDSSYYFAGVL
                     VAHYGV"
BASE COUNT          321 a          191 c          190 g          349 t
ORIGIN      
        1 caaatgcata atatgcatgg ccgctgccaa ttaactgttc agcatattgt ttatacaatg
       61 gcttacgttc gctttggcgg tatggcgcat gtttgccatc gccaaaacca acaccttcgt
      121 tcggaataat gccacaccag tttaatgcat caattatgta ttgctcggca ccgggtacaa
      181 aacggttttg gtcggtatct tcgatgcgta aaataaaatc gccaccatgt tgtttggcaa
      241 aaaggtaact gtataaagca gttcgcacac cacccatgtg caatgggcct gtaggactgg
      301 gcgcaaatcg aagtctaact ttatttgatt ccatttttat aggttatggt ttttaatgag
      361 ggcggcaaat atagtgttat gtgtaatggc accatgcaga agtgtgttag cgattcgcaa
      421 ataaattaac gcaccgagca aattgaaaga catttaataa cctattcgtt ttttaattcc
      481 tattttacat tctaaccttt ttgtatatga aaaaatttct atcatcctca ataatttatt
      541 ttgtattatt tgcagtaatt ctcacgatca gtatttgtat aatattacca gagttgacag
      601 cttaaacaat ttttttggtt tcgagcaaac ccttcttaat cctaagaata ataataaagt
      661 tgatttatcc atgaattaca aaccaggcta tgaagcccca ttattattga ctgaaatgga
      721 tagttctgga ataattttaa aaagcaaaaa gattaagttt tttagtgact cattacaatt
      781 tttctgcaac ggagcaaggt ggccatcagg cactcaattt gctttagcaa aaagtttata
      841 ctcccctatt gatagttcct attattttgc aggtgttctt gtggcacact atggcgtttg
      901 aagtacactg gcaactaacg cattactcaa agttgataat aatttggaca cagtatggct
      961 tcgtgaatat tatactgccg catatactgg gctcaacatt tctgctgata ctggattgat
     1021 agcttcagca ttatataaca taagaaagtt a
//
LOCUS       DAOMAA010000993         1051 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_197671_length_1051_cov_2.187751, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000993 DAOMAA010000000
VERSION     DAOMAA010000993.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_197671_length_1051_cov_2.187751"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="PLO59_11325"
     CDS             complement(<1..383)
                     /locus_tag="PLO59_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008237237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HQV01746.1"
                     /translation="MTDATDLRSIVTVTMILFAVIDIIGSVPVILDIRRKVGSIHPEK
                     VTIVSLCLMITFLFLGETILSFIGIDIPSFAIAGALILFFLSLEMILGIKLYKDEAPA
                     TASIVPIAFPLIAGAGTMTTLLSLRS"
     gene            509..>1051
                     /locus_tag="PLO59_11330"
     CDS             509..>1051
                     /locus_tag="PLO59_11330"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQV01747.1"
                     /translation="MYDIIVIGGGIVGTAAALKLNTHHPHLKILVLEKDTIAAHQTGH
                     NSGVIHSGLYYKPGSYKAKNCVDGRRELVAFAKQYHIKHDICGKIIVATHQSELPYMN
                     KAFNNGLQNGVEGIEKINAQQIQDIEPFARGIEGIWVPCTGIIDFAQVARTYVQIIEQ
                     QFAGSKVLENHEVKGFEHHTD"
BASE COUNT          355 a          186 c          217 g          293 t
ORIGIN      
        1 gaacgtaacg atagtaaggt tgtcatggtg cctgcacctg caataagcgg aaatgcaatg
       61 ggtacgatac tggctgttgc cggtgcttca tctttataaa gcttaatacc taaaatcatt
      121 tcaagcgata ggaaaaacaa tatcaatgca cctgcaatgg caaagctggg tatatcaata
      181 ccaataaacg ataaaatggt ttcgcccaaa aacaaaaacg taatcatcag gcataacgat
      241 acaatggtaa ctttttcggg gtgtatggag cctactttac gcctgatatc taaaattacc
      301 ggtacggaac cgatgatatc aattacagca aagagtatca ttgtaacggt tactatactt
      361 cgtaggtcgg tggcatctgt catatcgggc aaagatagta aagccatttt ttattttttt
      421 tacgtaacgt aaaacaatgg ttaaatcatt ttaataaagg cctaacaaaa tattttcagt
      481 gtgctgtgct gtgttatctt tgcgccctat gtacgatata attgtaatag gtggtggtat
      541 tgtaggtact gcagcagcat tgaagctaaa tacgcatcac ccgcatttaa aaattttagt
      601 gctcgaaaaa gataccattg ctgcacatca aaccgggcat aactccggag ttatacactc
      661 gggtttgtat tataaacccg gttcatacaa agcaaaaaat tgtgtagatg gcaggcgcga
      721 gttagttgct tttgcaaaac aatatcacat caaacacgat atatgcggta aaattattgt
      781 agccacgcat caatcggaat tgccttatat gaacaaggct tttaataatg gattacaaaa
      841 tggtgttgaa gggattgaaa aaattaatgc acaacaaata caagatatcg aaccatttgc
      901 acgcggcata gaaggaattt gggtgccttg taccggtata attgattttg cacaggtggc
      961 acgtacctat gtgcaaataa ttgagcaaca gtttgcaggt agtaaagtac tcgaaaacca
     1021 cgaagtaaaa ggctttgagc atcatacaga t
//
LOCUS       DAOMAA010000994         1050 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_197925_length_1050_cov_3.455276, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000994 DAOMAA010000000
VERSION     DAOMAA010000994.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_197925_length_1050_cov_3.455276"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="PLO59_11335"
     CDS             <1..225
                     /locus_tag="PLO59_11335"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017256746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HQV01748.1"
                     /translation="LAFGMMFPNMLIYLYFLIPVKAKYFVIGYGLLELYTSMQNNPGD
                     NVAHLAHLGGMIFGFIMIMMWRKKDNNFNY"
     gene            264..>1050
                     /locus_tag="PLO59_11340"
     CDS             264..>1050
                     /locus_tag="PLO59_11340"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HQV01749.1"
                     /translation="MQLIVDIKNRFQNGDVLTRLILVNIAVFLLINFGKLIAWFGLFD
                     GQWMDVIVHKLSFAEYLPVLITQPWSIITYMFTHESLSHIFWNLIVLYWVGQLILEYM
                     GEKKLWAIYFLGGLCAIIFMWVSLYILQFAGTISGRNQFLITPHAYALGASGSINACL
                     VAIATLIPNYTLYLFLIGPVRLKYVALVVILLSAISIPNGNAGGEIAHLGGALFGYLY
                     TKQLQQGRDVGAWFNKLSRWRLKPKSKMKLAHKSKEPKSNMHYD"
BASE COUNT          322 a          164 c          206 g          358 t
ORIGIN      
        1 ctagccttcg gaatgatgtt tcctaacatg ttaatctatc tgtattttct tattcctgtt
       61 aaagctaagt actttgttat tgggtatggc ttacttgaat tatatacaag tatgcaaaac
      121 aatccgggcg ataacgttgc acatttggcg catttaggtg ggatgatatt tggttttata
      181 atgattatga tgtggcgtaa aaaggataac aactttaatt attaatagca tcaaatgcat
      241 tttaaaaaca agcttcctta gttatgcaat tgattgttga cataaaaaac cgatttcaaa
      301 atggcgatgt gcttacacgt ctcatactgg taaacattgc tgtgttttta cttattaatt
      361 ttggcaaact aatagcgtgg tttggcttat ttgatgggca atggatggat gtgattgtac
      421 acaagctttc ttttgccgag tacttacccg tattaattac acagccctgg tcaataataa
      481 cctacatgtt tacacatgaa agtttgagtc atattttttg gaacttaatt gtattgtatt
      541 gggtaggcca attgatatta gagtacatgg gagaaaaaaa actatgggct atatattttt
      601 taggaggctt atgcgcgata atatttatgt gggtttcttt atacatccta caatttgccg
      661 gaaccatatc aggacgcaat caatttttaa ttacaccaca tgcttatgct ttaggagcat
      721 caggtagtat taacgcatgt ttggttgcta ttgcaacgct tattcctaac tatactttgt
      781 atttattttt aattggccct gtacgcttaa agtatgtagc attggtagta attctgctgt
      841 cagcaattag tattcccaac ggtaatgcag gtggcgaaat agcacattta gggggcgctt
      901 tatttggcta tttatatacc aaacaattgc aacaagggcg cgatgtgggt gcatggttta
      961 ataaattaag caggtggcga ttaaaaccaa aaagcaaaat gaaacttgca cataaaagca
     1021 aagaacccaa atcaaatatg cactatgacg
//
LOCUS       DAOMAA010000995         1049 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_198263_length_1049_cov_3.285714, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000995 DAOMAA010000000
VERSION     DAOMAA010000995.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_198263_length_1049_cov_3.285714"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1049)
                     /locus_tag="PLO59_11345"
     CDS             complement(<1..>1049)
                     /locus_tag="PLO59_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01750.1"
                     /translation="SYPKKYYAFLIAACLLMVAAFVYEAVYLKKLNNRIAIQQRVQGV
                     MDAKAQRINTFMKQVAMQLENNNTTTLNKQLQQLKSPMSDFVFFIYKNGQTIYWSNNS
                     VELGFNDKAHIVNEQIGYWASTYYEFFKQKTNNYQITGLLFIKRSQAGFENKYIVNQF
                     NSDFELTDNVIFASNKSTKQNFEIVNSRKQTLFSLLFEGEAITPVSIAIPYLFFLSLA
                     CLAIWLYTFVNYYIRTEWVFAFVCLLAVMVTYYLTFVVFKLPRSLFELTLFSPLLYAS
                     GNLLGSMGHLIMITMWLLFIALIIYRNISLINYNGLVLENQKIKLLSVFAVFFCMPLY
                     GTLIHYLIRGLILNS"
BASE COUNT          366 a          168 c          180 g          335 t
ORIGIN      
        1 ttagagttta atattaagcc gcgtatcaaa taatgtatta atgtgccata cagtggcata
       61 caaaaaaata cagcaaatac acttaataat tttatttttt gattttctag aacaaggcca
      121 ttgtaattta tcaaactaat gtttcggtag atgattaagg caataaataa taaccacatc
      181 gttatcatta ttaaatgacc catagagccc aataagttac cacttgcata aagcagaggc
      241 gaaaacagcg ttagctcaaa aaggctgcga ggtaatttaa aaacaacaaa agtcagataa
      301 taggttacca taacggccag caagcataca aatgcaaaaa cccattcggt tcggatataa
      361 tagtttacaa aagtgtaaag ccatattgcc aaacaagcca aacttaaaaa gaataaataa
      421 ggaattgcaa tactgacagg tgtaatggct tcgccttcga aaagcaatga aaaaagtgtt
      481 tgcttacgcg agtttacaat ttcgaaattt tgtttggtgg atttattgga tgcaaaaatt
      541 acattgtcgg ttagttcgaa atcgctgtta aactgattta cgatgtactt gttttcgaaa
      601 cctgcctgac tacgttttat aaataacaaa ccggtaatct gatagttgtt tgttttttgt
      661 ttaaaaaact catagtaggt agatgcccaa tagcctattt gttcattcac aatatgtgcc
      721 ttatcattaa aaccaagctc aacactgttg ttactccaat aaatagtttg gccgttttta
      781 tatataaaaa aaacaaaatc gctcataggc gattttagtt gttgcaattg tttgtttagg
      841 gttgtggtgt tgttgttttc taattgcatg gcaacttgtt tcataaaggt gtttatgcgt
      901 tgcgctttag catccattac accttgcaca cgttgttgta ttgcaatacg gttattaagt
      961 ttttttaagt aaacagcttc ataaacaaat gcagccacca ttagcaaaca agcggcaatt
     1021 aaaaaagcat aatatttttt aggatacga
//
LOCUS       DAOMAA010000996         1049 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_198267_length_1049_cov_3.218310, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000996 DAOMAA010000000
VERSION     DAOMAA010000996.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_198267_length_1049_cov_3.218310"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1049
                     /locus_tag="PLO59_11350"
     CDS             <1..>1049
                     /locus_tag="PLO59_11350"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01751.1"
                     /translation="GTTTISPTIIYYKDTQLVVLNSYPLPNVTFQDSLIYYLPPLEPY
                     NDTTIIIEFIPNSNLKPAYIKLKARIEPIANDNNPSDNFSEWIFSEASINYGNFAHSP
                     TKILVDQLNPTPPFIDYVVYFKTKYHRNQFYFSIQNVMAGNLAQHTFELVGSSNPVNI
                     DFNKEGKKIFIEMPHCNLIKSKFSGPRNYAWVHYRVKPKKTLKSGDVITNVINSNTEN
                     YDFYDANTFIVSPPDYFATAIQGDTTACIDTGLVHNYATPLHTGNIYNWTVPNYGSII
                     SGQGTHAIDVKWMATGAKYVAVVEKDSAMIFVDYDSVKVTTNPTYSKVNIVYLWPGDS
                     VFLAGMWQHTPGTYV"
BASE COUNT          349 a          192 c          173 g          335 t
ORIGIN      
        1 tggcacaact accatatcgc caactattat ttattataag gatacacaat tggtcgtgct
       61 aaattcttat ccgttaccta atgttacttt tcaggattcg ttgatttatt atttaccccc
      121 attagaaccc tataacgata caacaattat cattgaattt atacctaata gtaatttaaa
      181 acccgcgtat attaaactta aagcaaggat tgagcccatt gccaacgata ataatccttc
      241 agataatttt tcggaatgga ttttttcaga agcctccatt aattatggca attttgccca
      301 cagccctaca aaaatactcg ttgatcagtt aaacccaacg cctcctttca tagattatgt
      361 agtttacttt aaaaccaaat atcatagaaa ccaattctac ttcagtatcc aaaacgtaat
      421 ggccggaaat ttggctcaac acacatttga attggttggc agttctaatc ctgtaaatat
      481 tgatttcaat aaagaaggta aaaaaatatt tatcgaaatg ccgcattgta atcttatcaa
      541 atcaaaattt tcaggcccgc gtaattatgc ttgggttcat taccgggtga aacctaaaaa
      601 gactttaaaa tcaggagatg taattacaaa tgttattaat tctaatacag agaattatga
      661 tttttatgat gctaacacat ttatagttag cccacctgat tattttgcta ctgcaataca
      721 gggtgataca actgcatgta ttgatacagg tttggtacat aattacgcta caccactgca
      781 cacaggcaat atttataatt ggactgtacc taattacgga agcataatta gcggacaagg
      841 tacccatgca atagatgtaa aatggatggc tacaggtgca aagtatgttg cagttgttga
      901 gaaagactct gctatgattt ttgtagatta tgactcggta aaggttacaa ctaatccaac
      961 ttatagtaag gtaaatattg tttacttatg gccgggcgac agtgtttttt tagccggcat
     1021 gtggcaacac acaccaggca cttatgttg
//
LOCUS       DAOMAA010000997         1048 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_198640_length_1048_cov_3.330312, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000997 DAOMAA010000000
VERSION     DAOMAA010000997.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_198640_length_1048_cov_3.330312"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            347..>1048
                     /locus_tag="PLO59_11355"
     CDS             347..>1048
                     /locus_tag="PLO59_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015363223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HQV01752.1"
                     /translation="MAEGENIIKINIEDEMKTAYIDYSMSVIVSRALPDVRDGLKPVH
                     RRVLYGMLDLGVLSNRSYKKSARIVGEVLGKYHPHGDSSVYFTMVRMAQDWSLRYTLV
                     DGQGNFGSMEGDPPAAMRYTEVRMRKLAEEMLADIEKDTVDLRNNFDDTLQEPTVLPS
                     RFPNLLVNGSSGIAVGMATNMAPHNLTEALNGVIAYIENKDITIAELMQHLKAPDFPT
                     GGIIYGHSGVKDAYET"
BASE COUNT          330 a          194 c          216 g          308 t
ORIGIN      
        1 tttggcaagg gaaattttca ggctcttttc gaaagcatag aagccgaaca agcacgcaga
       61 ggtactttgt aaccggtacc taacttattt atctaacaaa gtaaatcgcg tttacgggtt
      121 agtaaatggc cgaatcaagc tgtaatttat gctgttgttt tataaagtca gatatgattt
      181 tataggcata ggtatttgtt agtactttat gataatctta gttaaatacg cttttatata
      241 cattaaaaat gtacgttatt cgcgcgggtt aaaaaacctg caaagcctat aaacattggc
      301 tattagcaaa acaaagacag attaatcaca ctaagcaaaa atacctatgg ccgaaggcga
      361 aaacattatt aaaattaaca tcgaagacga gatgaaaacc gcctacattg attattcaat
      421 gtcggtaatt gttagtcggg ccttacctga tgtacgtgat ggtttaaaac cggtacaccg
      481 cagggtactt tatggtatgc tcgatttagg tgtgcttagc aacagatctt ataaaaaatc
      541 agctcgtata gttggcgaag ttttgggtaa atatcatcca catggcgact catccgttta
      601 cttcaccatg gtacgtatgg cccaagattg gagcttacgt tataccttgg ttgatggcca
      661 aggtaatttt ggcagtatgg aaggcgatcc accggctgct atgcgttaca ccgaagtacg
      721 tatgcgcaaa ttagccgaag aaatgttagc cgatattgaa aaagataccg ttgatttaag
      781 aaacaatttt gatgatacac ttcaggaacc cactgtttta ccatcgcggt ttcctaattt
      841 attggtaaat ggttcatcag gtatcgctgt gggtatggcc accaatatgg caccacataa
      901 ccttactgaa gccttaaatg gtgttattgc ttacatcgaa aataaagaca tcaccattgc
      961 cgagttaatg caacacctaa aagcacccga ttttccgaca ggcggtatta tttatggcca
     1021 tagcggtgtt aaagatgctt atgaaacc
//
LOCUS       DAOMAA010000998         1048 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_198687_length_1048_cov_2.681772, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000998 DAOMAA010000000
VERSION     DAOMAA010000998.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_198687_length_1048_cov_2.681772"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /locus_tag="PLO59_11360"
     CDS             complement(<1..963)
                     /locus_tag="PLO59_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01753.1"
                     /translation="MRKTFLKTGSDPVYNLNILTSDLNYQMAMLYHARELNLPVRNVE
                     LGNEFYSNDENNNLKFPTVIDYADEAISWATSIKSDAGFATNPPLIAAVGANASYGIA
                     ATDAGMTVYNNTFTALSPPVRGAINAQQPNYYNEKKIIIGKENSFAKRNVLTDNLVGI
                     TISGEYNVAIGRNSFNKDLTDRNTDIFIENNLSKPVDIAYNRIDDFVYGIKILNAGHT
                     SATHPWITIHDNQITQAALTTGTYGIGINVSNTTQQTSQVAIYDNYILEPRIGISMSK
                     VDGAKIYKESTSNKIQFSFNATQGISPVHAGIELNECDNVFVHEN"
BASE COUNT          253 a          195 c          229 g          371 t
ORIGIN      
        1 gttttcatgt acaaaaacat tatcacactc atttaattca atgcctgcat gtacaggtga
       61 aatgccctgc gtggcattaa aactaaattg aattttattg cttgtgcttt ccttataaat
      121 tttagcccca tctacctttg acatggatat gccaatgcgt ggctctaata tgtagttgtc
      181 ataaatcgcc acctgcgagg tttgctgtgt ggtgttgctt acatttatgc ctatgccgta
      241 agtgcctgtg gttagtgctg cttgggttat ttggttgtcg tgtatggtta tccacgggtg
      301 ggtggcagag gtgtggccgg cattaagaat ttttatgcca tacacaaaat cgtcaatccg
      361 gttgtaggct atatcaacgg gtttgctcag gttgttttca ataaagatgt cggtatttct
      421 gtcggtaagg tctttgttaa aactattgcg gcctatggct acattgtatt cacctgaaat
      481 tgttatcccc acaaggttgt cggttagtac atttcgtttc gcaaagctgt tttcttttcc
      541 aataattatt ttcttttcat tgtaatagtt tggctgttgt gcatttatgg ctcctctaac
      601 aggcgggcta agcgcagtaa aggtgttgtt atacaccgtc atgccggcat ctgtggctgc
      661 gatgccgtag ctggcattgg cacctactgc ggcaattagt ggcgggttgg ttgcaaaacc
      721 ggcatcgctt tttattgatg tagcccaact tatggcctca tcggcataat caattacagt
      781 aggaaatttt aaattattgt tttcgtcatt tgaataaaac tcattaccaa gttccacatt
      841 acgtacaggt aaatttaatt cgcgtgcatg gtaaagcatc gccatttgat aatttaagtc
      901 agaggtaagt atgtttaaat tatacacggg atcgctgcct gttttcaaaa atgtttttcg
      961 catttccgta agcgtgttta tgggcttgca aacccaattg taattggtat tgtaccaatt
     1021 tttagtcaat gcttcaaaat tgtttggc
//
LOCUS       DAOMAA010000999         1046 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_199529_length_1046_cov_2.309788, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010000999 DAOMAA010000000
VERSION     DAOMAA010000999.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_199529_length_1046_cov_2.309788"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /locus_tag="PLO59_11365"
     CDS             <1..833
                     /locus_tag="PLO59_11365"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015809619.1"
                     /note="an AccC homodimer forms the biotin carboxylase
                     subunit of the acetyl CoA carboxylase, an enzyme that
                     catalyzes the formation of malonyl-CoA, which in turn
                     controls the rate of fatty acid metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HQV01754.1"
                     /translation="IVWKAEDLEPAWDSARQEAGAAFGNDGMYMEKYIEEPRHIEIQI
                     AGDQYGKACHLSERDCSIQRRHQKLLEETPSPFMTAQLREAMGEAAIKAAMAVKYEGV
                     GTVEFLVDKHRNFYFMEMNTRIQVEHCITEEVINYDLIKEQIKIAAGVPISGKNHYPA
                     MHAIECRINAEDPLNGFRPCPGKITNLHVPGGHGVRVDSHVYASYTIPSNYDSMISKL
                     ITIAQTREEAIQTMERALSEYVIEGVKTTIPFHQKLMQDENFRKGNYTTKFMESFKYD
                     "
BASE COUNT          370 a          197 c          215 g          264 t
ORIGIN      
        1 gtattgtttg gaaagccgaa gatttagagc ctgcctggga ttcggcaaga caagaagccg
       61 gtgctgcatt tggtaatgat ggaatgtata tggaaaaata cattgaagaa ccgcgacaca
      121 tcgaaattca aattgcaggc gaccaatacg gaaaagcatg tcacttaagc gagcgcgact
      181 gctccataca acgcagacat caaaaactac tcgaagaaac gcccagtcct tttatgactg
      241 cacaactacg tgaagctatg ggcgaagcgg ctattaaagc agccatggca gttaaatacg
      301 aaggtgtggg tactgttgag tttttagttg acaagcaccg taacttctac tttatggaaa
      361 tgaatacacg cattcaggtt gaacattgca taacagaaga agtaattaat tacgatttaa
      421 taaaagagca aattaaaatt gctgcaggcg tgcctataag tggtaaaaat cattatccgg
      481 ccatgcatgc cattgaatgt cgcataaatg ccgaagatcc attaaacggt tttaggcctt
      541 gtccgggcaa aataaccaac ctgcatgtac cgggcggcca cggtgttagg gttgactcgc
      601 atgtatatgc cagctacaca atcccttcaa attatgacag catgatttcg aagctgatta
      661 ctatagcaca aacacgcgaa gaggccatac aaacaatgga acgtgcatta agcgaatatg
      721 taattgaagg tgtaaaaaca accatcccat ttcatcaaaa actgatgcaa gacgaaaact
      781 tcagaaaagg taattacact accaaattca tggagtcgtt taagtacgat taagctacat
      841 taacattaaa taaaaacccc cgcttacatt ctaaggcggg gtttttttat ttcttataat
      901 cagttttgta cggatgtttt aggtaaataa cgctctaaaa aaaaagtacc aaagggcaca
      961 atagaaagca aaaataaagt aaccatagtt gtgattttta atttttgttt ggtgtgcaag
     1021 cctgctaata aaacaacaaa agaaat
//
LOCUS       DAOMAA010001000         1045 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_199860_length_1045_cov_2.763636, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010001000 DAOMAA010000000
VERSION     DAOMAA010001000.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_199860_length_1045_cov_2.763636"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="PLO59_11370"
     CDS             <1..312
                     /locus_tag="PLO59_11370"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV01755.1"
                     /translation="GCDSIIISNVVIANGINMLTDVDMQLHPNPANTHTTLTFVQCAN
                     KFVTVTIYDNVGKTCFNKQLKLDPAGKYNLETGNLAKGIYSIRIKHGTKMFYKKLIKY
                     E"
     gene            401..964
                     /locus_tag="PLO59_11375"
     CDS             401..964
                     /locus_tag="PLO59_11375"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HQV01756.1"
                     /translation="MKKIICWLIIGFGVMSVTAYNKQAVPKQSKQNASIETGAVIISE
                     VLSNGSDYYNELILPGNPNGGADWLELYNTTNDTIVIKANQWFITDSFLHPQKFALPP
                     MLLLPNMHQVIWCDGMDTTLTQIHSNFKLNKRGERLALYYKNTNQTLTQVNSITFGSI
                     KARQSLVRKHAHAVATKCKKPTPGYSI"
BASE COUNT          385 a          190 c          173 g          297 t
ORIGIN      
        1 ggatgcgata gcattattat ttctaatgtg gtaatagcta acggtattaa tatgcttact
       61 gatgttgata tgcagttaca tccaaatccg gctaacacac ataccacact aacatttgtg
      121 caatgcgcca ataagtttgt aacagttact atatacgaca atgtaggaaa aacatgtttt
      181 aacaaacaac ttaaattaga ccctgccggt aaatacaact tagaaactgg caacttggca
      241 aaaggcattt atagcattcg aattaaacat ggaacaaaaa tgttttataa aaagctaata
      301 aaatacgaat agctaaatta attgtatcca tataaaaagc tttacaattt cttaatacag
      361 aagcaataca tgttttataa gcgatgaagt gctttgcatt atgaaaaaaa tcatctgttg
      421 gttgataatt ggctttggtg taatgtcggt aactgcatat aacaaacaag cagtacccaa
      481 acaatcaaaa caaaacgcat caatcgaaac cggtgccgta ataataagcg aagtgctatc
      541 aaacggttcg gattattaca atgaattgat attgcccggc aacccaaatg gtggtgccga
      601 ttggttggag ttgtataaca ctaccaacga taccatagtc attaaagcaa atcaatggtt
      661 tattaccgat tcgtttttac atccacaaaa atttgcatta ccgccaatgt tactgttacc
      721 taacatgcat caggtaatat ggtgcgatgg tatggatact accttaaccc aaatacacag
      781 taattttaaa cttaataaaa ggggcgagcg cttagcttta tattataaaa ataccaatca
      841 aacacttacg caagttaaca gcataacatt tggctcaata aaagcacgac aaagcctggt
      901 aagaaaacac gcacatgctg ttgcaacaaa atgcaaaaag ccaacaccgg gctacagtat
      961 ttaattgatt cttttccgaa acggcttttt ttaattaggg cttatagcca aaaaaagtat
     1021 cgtctttatc cagttcggtt tctaa
//
LOCUS       DAOMAA010001001         1045 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_199928_length_1045_cov_2.308081, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010001001 DAOMAA010000000
VERSION     DAOMAA010001001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_199928_length_1045_cov_2.308081"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="PLO59_11380"
     CDS             <1..182
                     /locus_tag="PLO59_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009391020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01757.1"
                     /translation="NKLESVRLYENFDYNKISSLSTEARQKLLKIKPTTLGQASRISG
                     VSPSDISILMVYLGR"
     gene            208..>1045
                     /locus_tag="PLO59_11385"
     CDS             208..>1045
                     /locus_tag="PLO59_11385"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQV01758.1"
                     /translation="METISNCPICNKNLFTPFITCTDYTVSQKDFQIVTCNECSFKFT
                     NPKPTNEDLGSYYKSNDYISHTNSDKGWFNKLYKTVRNYTIDKKVKALSKYNAPSKTL
                     LDIGCGTGEFLNAAKIKQWHVTGLEPGVDARNLAIKNYGLHVFDIDHLAKLKPQSFDV
                     ITMWHVLEHIPNLHETIKQLKQILAPNGTLIVAVPNSNAIEAKNFGRFWAAYDVPRHL
                     NHFSAKNVINLLKINNLDVCSTKPMIFDPFYIAMLSAKYQNKSPFFGLVLGIYHGLRT
                     NVF"
BASE COUNT          374 a          176 c          173 g          322 t
ORIGIN      
        1 tgaataagtt ggagtctgtt cggctttacg aaaattttga ctacaataaa attagctcct
       61 taagtacaga agccagacag aagttgttaa aaattaaacc aaccacttta ggccaagcaa
      121 gtcgaataag tggagtttcg ccttccgata tttctatcct gatggtttat ttgggacgct
      181 aataacaaac aatatttaaa atttttgatg gaaacaatta gcaattgccc aatttgtaat
      241 aagaatctat ttactccctt tataacttgc acggattaca cagtaagtca aaaagacttt
      301 caaattgtaa cttgcaatga atgcagtttt aagtttacta acccgaaacc aactaacgaa
      361 gatttaggtt catactataa atcaaacgac tatatatcac acacaaattc agataagggt
      421 tggtttaata aattatataa aaccgttaga aattacacaa ttgataaaaa ggtaaaagct
      481 ttaagtaaat acaacgcccc atcaaaaacg ctattagata ttggatgtgg tacaggcgaa
      541 tttttgaatg cagctaaaat taagcaatgg catgttaccg gattagagcc aggtgttgat
      601 gcacgcaatt tggcaataaa aaactatgga cttcacgtgt ttgatatcga ccatttagcc
      661 aagcttaagc cacaaagttt tgacgtaata acaatgtggc atgttttaga gcatattcca
      721 aacttgcatg aaactattaa acagttgaag caaattttag cacctaatgg tactttaatt
      781 gtagctgtac caaatagtaa tgcaattgaa gcaaaaaatt ttggtcgctt ttgggcagct
      841 tatgatgtac ccagacattt aaatcatttt tcagccaaaa acgtaattaa ccttctgaaa
      901 atcaacaact tagacgtgtg ctctacaaaa cctatgattt ttgacccctt ttatattgct
      961 atgctaagtg caaaatatca aaacaaatcg ccattttttg gtttggtatt gggtatctat
     1021 catgggttaa gaacaaacgt tttct
//
LOCUS       DAOMAA010001002         1045 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_200038_length_1045_cov_1.828283, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010001002 DAOMAA010000000
VERSION     DAOMAA010001002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_200038_length_1045_cov_1.828283"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="PLO59_11390"
     CDS             <1..267
                     /locus_tag="PLO59_11390"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQV01759.1"
                     /translation="KTDSNNISANIILGKLAIQSGQFDKAIKRLELVLSLKPDNTEAL
                     YFLAEAYKGSGNKQKAISLFEQCKALVNNPEFTKEIDNYITTFK"
     gene            498..>1045
                     /locus_tag="PLO59_11395"
     CDS             498..>1045
                     /locus_tag="PLO59_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HQV01760.1"
                     /translation="MNKELVIQSIDSGIEIALLEDKKLVELHFDNSGGSFNVGDMYLG
                     KVRKIMPGLNAVFVDIGHDKDAFLHYTDLSPYFKSLLKFTEMAIHDKSGGSFDFSKFQ
                     LEPEIVKTGKVADIMQGKPNVLVQILKEPISSKGPRVSCEISLPGRFIVVTPFNNFVN
                     VSRKIHSTEERKRLQKIIESIKP"
BASE COUNT          386 a          175 c          182 g          302 t
ORIGIN      
        1 aaaaccgaca gcaataacat ttctgcaaat atcattttag gaaagttagc gatacagtca
       61 gggcaatttg acaaagccat caaaagatta gaattagttc tttcgttgaa accggataat
      121 accgaagcat tgtacttctt agccgaagca tacaaaggaa gtggcaacaa gcaaaaagcc
      181 atcagtttgt ttgaacaatg caaagcattg gtcaataatc cagaatttac aaaagaaata
      241 gataactata taacgacatt taaataattt ttttaacaaa aacattttaa tcatgccttg
      301 cggaaaaaaa cgtaaaagac acaaaattgc aacccataaa cgtaaaaaac gtttaagaaa
      361 gaaccgtcat aagaaaaaat aattttctta ggataatagg cggtaactca tcaatgcatg
      421 tattttattt tttataaata aaatacatgt ttgttatagt gtgtgtatga ttattgttgt
      481 aagaaaccat tacagctttg aacaaagaac tcgtcattca atccatcgat agcggtattg
      541 aaattgcact tttagaagat aaaaagctcg tagagctaca ttttgacaat tcaggtggaa
      601 gttttaacgt cggtgatatg tacctcggca aagtacgcaa aattatgcct ggcttaaatg
      661 ctgtttttgt cgatattggt catgacaaag acgccttttt gcattatact gatttaagcc
      721 cttactttaa gagtttattg aaatttaccg aaatggccat ccacgataag tcgggtggga
      781 gtttcgattt ttcaaaattt caactcgagc ccgaaatcgt caaaacgggt aaagtagctg
      841 atatcatgca aggcaaaccc aatgtactgg ttcaaatact caaagaacct atttcatcaa
      901 aaggaccaag ggttagttgc gaaatatcat tgccaggccg atttattgta gtaactcctt
      961 tcaataactt tgtcaatgtg tcgcgcaaaa tacattctac agaagaacgt aagcgattgc
     1021 aaaaaattat tgaaagtatc aaacc
//
LOCUS       DAOMAA010001003         1044 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_200222_length_1044_cov_3.259858, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010001003 DAOMAA010000000
VERSION     DAOMAA010001003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_200222_length_1044_cov_3.259858"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="PLO59_11400"
     CDS             <1..185
                     /locus_tag="PLO59_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01761.1"
                     /translation="NIMPTFLKAPFQNAFKKVDNMYMERNTTNYDELILEPTMYAKLE
                     RYYEADQVWVKSIIKH"
     gene            213..>1044
                     /locus_tag="PLO59_11405"
     CDS             213..>1044
                     /locus_tag="PLO59_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01762.1"
                     /translation="MKLKISNVVIGLAVYLPFESMILKLLPLPDSIVSFFHFGIEVVI
                     YVLFLTLLLRNMSMGKFFHKTIADVPYLTFLCFAIFTMIVNHAPVSESLVDLRGLLKY
                     SLLYFVVANIDFSPKSARTLLNAFVVVAFFQCLIVTFQHFFGIAEFWYPRANELEIAG
                     KTTNAKLLVEGFKGGREQGAGIGTFGDTVPLANYAVITLTLLTPMLLGFIRVNAYRMV
                     FLIVTWFLVLAALFFSYSRGSILMGLASIPLTFLLSRNLHKFFPMFLAVALFLTAFIG
                     Y"
BASE COUNT          297 a          183 c          190 g          374 t
ORIGIN      
        1 aaaacataat gccaaccttt ttaaaagcac catttcagaa tgcttttaaa aaggttgaca
       61 atatgtatat ggagcgaaat acgacaaatt atgatgagct aattttggag ccaacaatgt
      121 atgccaaatt agaaaggtat tacgaggccg atcaagtgtg ggttaaatca atcataaagc
      181 actgatattc aatatttcaa cctcattttt acatgaaact taaaatatca aatgtggtaa
      241 tagggttggc tgtttatttg ccattcgaat ccatgatatt aaagcttttg cctttacccg
      301 attcaatcgt aagttttttt cattttggta tcgaagtcgt tatctacgta ttattcctaa
      361 cacttttgct ccgaaatatg agcatgggca agttttttca taaaactatt gccgatgtgc
      421 cctatttaac ctttttatgt tttgctatat ttacaatgat tgtaaatcat gcacccgtaa
      481 gcgaaagctt ggttgacctg cgtgggttac ttaagtactc actattgtat tttgttgtag
      541 ctaatattga ttttagtcca aaaagcgcac gtacattgtt aaatgcattt gttgtagtgg
      601 ctttttttca atgcttgatc gtaacgtttc aacacttttt tggcatagcc gagttttggt
      661 accccagagc taacgaatta gaaattgccg gaaaaacaac caacgctaaa cttttagtag
      721 agggctttaa aggcggccgt gaacagggtg caggtatcgg tacatttggc gatacggttc
      781 ctttggcaaa ctatgcagta attactttaa ctttattaac cccaatgcta cttggtttta
      841 ttagggtgaa tgcttaccgc atggtttttt taatagtaac ctggtttctg gtattagcag
      901 cactgttctt ttcttattca cgaggctcca ttcttatggg cttagccagc ataccattaa
      961 catttttact tagccgaaac ttgcataaat tttttccaat gtttttagcg gttgctttat
     1021 tcttaaccgc ttttattgga tata
//
LOCUS       DAOMAA010001004         1044 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_200442_length_1044_cov_1.924166, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010001004 DAOMAA010000000
VERSION     DAOMAA010001004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_200442_length_1044_cov_1.924166"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..950)
                     /locus_tag="PLO59_11410"
     CDS             complement(<1..950)
                     /locus_tag="PLO59_11410"
                     /inference="COORDINATES: protein motif:HMM:NF025720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4407 domain-containing protein"
                     /protein_id="HQV01763.1"
                     /translation="MNKLQQFFWFSAGVNITLLRKCPTEHNKYFALGATVVFTGLCAA
                     LSGGYALYDVFNDSVWYIRLMAAILLGLFWGALIYNLDRFLVLSIKKHHVWYKQWLMA
                     VPRLALAGIIAIVISKPLELQIFHTGINYQLALMHQTEIKKQETAVQQRYHAEKTEYQ
                     QKLQLLKTEISNKAATRDSLATEARQEADGSGGSKQRGASVIYNIKKADAVNAQAEYI
                     QTLAANTPQIKTLEAALHVQSQKILAEQTAIKPGRFDGFDKRLAALNQLTDNDNSIAL
                     AHTFIMWLFFAIETAPVWVKLMSGRGPYDDLLQLHEHHFEV"
BASE COUNT          292 a          225 c          195 g          332 t
ORIGIN      
        1 acttcaaaat gatgttcatg taattgcaat aaatcatcat aagggccacg gccactcatt
       61 aactttaccc aaaccggagc tgtttcgata gcaaaaaaca accacatgat aaatgtatgc
      121 gctaaggcta tactgttatc gttatcggtt aactgattaa gcgcagccaa tcgtttatca
      181 aagccatcaa agcggccggg cttaatagct gtttgctcgg ctaatatttt ttggctttgt
      241 acatgcaatg ccgcttcaag cgttttaatt tgaggtgtgt ttgctgcaag cgtttgtata
      301 tactcagcct gcgcattaac ggcatcggct ttttttatat tataaattac cgaagcgcca
      361 cgttgtttac taccgccact gccatcggcc tcttgcctgg cttctgtggc caaactatcg
      421 cgtgtagcag ccttgttact tatttcggtt ttcagcaatt gcagcttttg ttgatattcg
      481 gttttttcgg catggtaacg ttgctgcacg gctgtttcct gtttttttat ttcggtttga
      541 tgcattaagg ccaactgata atttatgcct gtatgaaaaa tttgcaactc aagcggtttc
      601 gaaattacaa tagctataat gcctgccaat gccaaacgcg gtacggccat aagccattgt
      661 ttgtaccata catggtgttt ttttatgctg agtaccaaaa accggtcgag gttataaata
      721 agcgctcccc aaaacaaacc caataatatg gcagccatca aacgtatata ccaaacacta
      781 tcgttaaaaa catcgtataa tgcataaccg cctgataagg cagcgcataa acctgtaaaa
      841 acaacagtag cacccagtgc aaaatattta ttatgttcag tggggcactt gcgcaacagg
      901 gtaatattta cacctgcact aaaccaaaaa aactgctgta atttattcat ggcttgtact
      961 ttttttattt gaataccatt gctggcgtat gttgtttttt ataacacttg ctttcttttg
     1021 gtaagcatgc aactcatcaa tacc
//
LOCUS       DAOMAA010001005         1043 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_200815_length_1043_cov_1.944332, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010001005 DAOMAA010000000
VERSION     DAOMAA010001005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_200815_length_1043_cov_1.944332"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="PLO59_11415"
     CDS             complement(<1..195)
                     /locus_tag="PLO59_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01764.1"
                     /translation="MKKIASIFLLTNALHSGANAQLLQNTIWSFYDPLNVFSFYFQFN
                     NDTVSLSTDNISYTPTSIYNE"
     gene            610..>1043
                     /locus_tag="PLO59_11420"
     CDS             610..>1043
                     /locus_tag="PLO59_11420"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HQV01765.1"
                     /translation="MKKIASLIALCLMFCTGVVAQSFIYGKKGGSLGHDAGTAMVSDN
                     NNNVYIAGYFADSIRFGNMKVTGLGVQDIFVVKLDPSGNAIWLKRFGGNKPDEAHAIA
                     IDYAGNLYLTGFAASDTAYFDTVKVACVTKQIFVAKLNSNGD"
BASE COUNT          334 a          149 c          206 g          354 t
ORIGIN      
        1 ttcattatag attgaggtag gcgtgtagct aatattgtcg gtacttaatg aaaccgtatc
       61 gttattaaat tgaaagtaaa agctgaaaac attaagcggg tcgtaaaagg accatatggt
      121 gttttgtaat aattgtgcat tcgcacccga atgcaatgca ttagttaata aaaagatact
      181 ggcaattttt ttcatttaat aaaattttaa agtgcagttt tgttatacat gattaaatgg
      241 tgatacaaat gtaaataatt taaaagagga aaatcattct ctggtaagca ggattatttt
      301 ttttgaactg gcatgtgact ttttttgtaa aaaagtgaaa tagaattgct gatgtttgtg
      361 tttttatttt agtttttaac ttttgcctta gtttcagcgt ctctcatgca attgaaaaac
      421 aaaagataaa tgaggactct gaaattcggt ttctgcgttt gcggagagga ttgttcttag
      481 tttcgctttt ggcaatttag tttttaactt ttgccttaat ccttttgaat taattaagca
      541 gctgtattgt cctttaacct gatttatata tttttactgc tcctaatcca acaaaacaat
      601 ttacatatga tgaaaaaaat agcaagtctg atagcattat gtttaatgtt ttgtacaggc
      661 gtggtggcac aatcatttat ctatggaaaa aaaggtggct cacttggaca cgatgcaggc
      721 actgctatgg ttagtgataa caataacaac gtttatatag caggttattt tgccgacagc
      781 atacgttttg gtaatatgaa agtaacagga cttggagtac aagacatttt tgttgtaaag
      841 ctagatccaa gtggtaatgc catttggctt aaacgatttg gaggaaataa acctgacgaa
      901 gcacatgcaa ttgccataga ctatgccggc aacttgtacc tcacaggatt tgccgccagc
      961 gatacggcat atttcgatac agtgaaagtg gcttgcgtaa caaaacaaat atttgttgcc
     1021 aaactaaata gtaacggtga ctg
//
LOCUS       DAOMAA010001006         1043 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_200899_length_1043_cov_1.534413, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010001006 DAOMAA010000000
VERSION     DAOMAA010001006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_200899_length_1043_cov_1.534413"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..724)
                     /locus_tag="PLO59_11425"
     CDS             complement(95..724)
                     /locus_tag="PLO59_11425"
                     /inference="COORDINATES: protein motif:HMM:NF017072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01766.1"
                     /translation="MKKLKIIVIREGKIPHDKRVPLAPMQIVSLQKMYPQIEVLVQPS
                     PWRSIPDVEYEVAGIKVTDAIADGDIFLGIKEVPVAELIEGKQYLFFSHTIKKQQHNK
                     KLMLAFIAQKIQMIDYECLVDDKENRIIGFGRYAGLVGAYNTIRAYGLKYKKFKIKPA
                     QDCEHKAELERELKKAKLPNLKILITGGGRVANGAIEIMGTMKIRRVTP"
     gene            complement(751..>1043)
                     /locus_tag="PLO59_11430"
     CDS             complement(751..>1043)
                     /locus_tag="PLO59_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HQV01767.1"
                     /translation="KTIKLAHIELDKLCQQAISKQQLKQYKQQLLGQIALAQENKASV
                     MIGLGRSMLNHGKVDTLKQLQEKIEAITSAQLLNVAHEIFDTKKHCSLLFIN"
BASE COUNT          283 a          198 c          180 g          382 t
ORIGIN      
        1 cgtttaccca tccggttttg ttgggatgat aacggtaatc gctgctatgc aattgggcat
       61 aaaccggctc gttaaacgga aacattaaaa actcctaagg tgtaacgcgt cttattttca
      121 tggtgcccat tatttcaata gcaccgttgg caacgcggcc gccaccagtt ataagtattt
      181 taaggttggg tagtttggcc tttttaagct cacgttctaa ttcagcttta tgttcgcaat
      241 cttgagcagg tttaatttta aattttttat atttcaaacc ataagcccta atggtgttgt
      301 atgcgccaac caaaccggca tatctgccaa aaccaataat gcggttttct ttatcatcaa
      361 ctaaacattc ataatctatc atttgaattt tttgtgctat aaatgccaac atcagttttt
      421 tattatgctg ttgtttttta atggtatgcg aaaaaaataa atattgtttt ccttcaatta
      481 attcagcaac cggtacttct ttaatgccta aaaaaatatc gccatcggca atagcatctg
      541 taacttttat accggcaact tcatactcaa catcgggtat ggaacgccac ggacttggtt
      601 gaacaagcac ttcaatttgc ggatacattt tttgcaaact cactatttgc ataggtgcca
      661 agggtacgcg tttatcatgt ggtattttac cctcgcgtat tacaataatt ttgagctttt
      721 tcatttcctt aataatctag tatcagtttg ctaattaata aacaacaaac tgcaatgttt
      781 tttagtgtca aatatttcat gcgcaacgtt taatagttgt gcgctggtta tagcttcaat
      841 tttttcttgc aattgtttta aggtatcaac cttaccgtga ttaagcatgc tgcggccaag
      901 cccaatcatt accgaagctt tgttttcttg cgccaatgca atttgtccta aaagttgttg
      961 cttgtattgt tttaactgtt gttttgatat ggcctgctgg cataatttgt ctaattcaat
     1021 atgggctaat tttatggttt tat
//
LOCUS       DAOMAA010001007         1042 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_201039_length_1042_cov_3.054711, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010001007 DAOMAA010000000
VERSION     DAOMAA010001007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_201039_length_1042_cov_3.054711"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..1007)
                     /locus_tag="PLO59_11435"
     CDS             complement(57..1007)
                     /locus_tag="PLO59_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01768.1"
                     /translation="MHYKIVVMHVMLFILPAMNKLTLARCSGRMANQIWMVANIIAFA
                     KENNLQVHAPFFLKIAANFEYWNHQTLITIDQKGIPNNWLRKYFYFLYQSGIMDLIKP
                     HVNLMPGPINKLLLRRKSQTIHKVYFNDTEYASVTNSIIANKHTLLSGFMFCSSVKLM
                     KENGGFLRHIFKPQSSVQLYLKNKISEIKAGHILVGVHIRRGDYKYYRNGAFYFPFEF
                     YAGKMVEIQQLLDKPVQFVLCSDETIPSELFKRFELLNHTKSDAVTDMYMLAKCAYII
                     APPSTYSKWASFYGEVLLANVSETPLTLKQFKVYYNHYEE"
BASE COUNT          325 a          184 c          172 g          361 t
ORIGIN      
        1 taatcaccca taatttaatt ctaaaaaaac ggctggctat gcaaccagcc gtttttttat
       61 tcctcatagt gattgtaata aaccttaaat tgttttagcg tcaatggagt ttcgctaaca
      121 tttgccaata aaacttcgcc ataaaacgat gcccacttac tgtaagtact gggtggggca
      181 attatataag cacactttgc caacatatac atatctgtaa cagcatctga ttttgtgtgg
      241 tttagcaatt cgaaacgttt aaatagctcg cttgggattg tttcatcact gcaaagcaca
      301 aactgtacgg gcttatctaa cagttgttgt atttcaacca ttttgccagc gtaaaattcg
      361 aacggaaaat aaaatgcacc attacgataa tatttgtaat cgcctctgcg aatatgtaca
      421 cctactaaaa tatggccggc tttaatttcc gaaattttat tttttaagta caactgtact
      481 gatgattgcg gcttaaaaat atggcgtaag aaaccgccat tttctttcat taattttaca
      541 ctactgcaaa acataaatcc tgataatagc gtatgcttgt ttgctataat actatttgtg
      601 actgatgcat attcggtatc attaaaataa actttatgta ttgtttgtga ttttcgtctt
      661 agtagtaatt tgttaatcgg accgggcatt aagtttacat ggggctttat caaatccata
      721 atacctgatt gatataaaaa ataaaaatac ttccgtaacc agttgttggg tatgcctttt
      781 tgatctattg taattagggt ttgatgattc caatactcaa aatttgccgc tatttttaaa
      841 aaaaaaggtg catgaacttg caggttgttt tcttttgcaa atgcaataat gtttgccacc
      901 atccatattt gattagccat acgaccactg caacgggcaa gggttaattt attcatagcc
      961 ggcaaaataa ataacattac atgcattaca actattttgt aatgcatggt tgtgtgcaat
     1021 attatatttg ccggctacct at
//
LOCUS       DAOMAA010001008         1042 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_201076_length_1042_cov_2.671733, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010001008 DAOMAA010000000
VERSION     DAOMAA010001008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_201076_length_1042_cov_2.671733"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..>1042)
                     /locus_tag="PLO59_11440"
     CDS             complement(145..>1042)
                     /locus_tag="PLO59_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HQV01769.1"
                     /translation="YVTTMLVQTGTIKTGDVLLAGCHSGRVKAMFNERGIRMEEAGPS
                     TPVMLLGLSGAPQAGDQFNVMEDEREARDIANKRLQLQREQGLRTHKHITLDEIGRRL
                     AIGNFKELNVIVKGDVDGSVEALADSIHKSVGQISESDVLLASASNAIIIGFQVRPSV
                     NARKLAENEQIDIRLYSIIYKAIEEIKAAMEGMLAPEFEEKIVCNIEIRETFKIPKVG
                     TIAGCMVLDGKITRNTKVRVVRDGIVTYSGELASLKRFKDDVKEVNAGYECGLNIANF
                     NDIQIGDIVEGYEQVAVKRTLA"
BASE COUNT          291 a          246 c          180 g          325 t
ORIGIN      
        1 gtatcacctc agcttaacta cggcaacaca tcgcactgta aaaaagtttg taaagcatta
       61 agcttacgaa gtaagaaagt tttattaaac aaaagcggct gtatcagtaa acgatacagc
      121 cgcttttgtt ttatttataa acgtttatgc caaggtacgt tttacagcca cttgctcata
      181 tccctcaact atatcaccaa tctgaatgtc gttaaaattg gcaatattta aaccgcattc
      241 gtaaccggca tttacctctt ttacatcgtc tttaaaacgt tttaaggaag caagttcacc
      301 actgtaggtt acaatgccat ctctaaccac acgcactttc gtattacgtg taatctttcc
      361 gtctaaaacc atacatcctg caatggtacc cactttaggt attttaaagg tttcgcgtat
      421 ttcgatgtta caaacaattt tttcttcaaa ttcaggcgcc aacataccct ccattgcggc
      481 cttaatttct tcgattgcct tgtaaataat agagtataat ctgatatcaa tttgttcgtt
      541 ttcggccagt ttacgtgcat taacagatgg gcgtacctgg aaacctatga ttattgcatt
      601 agaagctgaa gctaacaata catcggactc tgaaatctgt ccaaccgatt tatgtattga
      661 atcggccaag gcttcaaccg agccatccac atcacctttc acaattacgt taagctcttt
      721 aaaattacca atggcaaggc gtctgccaat ttcgtccagt gtaatatgtt tatgggttcg
      781 caaaccttgt tcgcgctgta attgcaaacg tttgttggca atatcgcgcg cttcgcgttc
      841 atcttccatc acattaaact gatcgcccgc ttgcggtgca ccacttaaac ccaatagcat
      901 taccggtgtt gatgggcctg cttcttccat acgtatgccg cgctcattaa acatggcttt
      961 tacacgtccg ctgtggcaac cggctaacaa tacatcaccg gttttaatgg tgcctgtttg
     1021 taccaacatg gtagtaacat ag
//
LOCUS       DAOMAA010001009         1041 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_201470_length_1041_cov_2.622718, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010001009 DAOMAA010000000
VERSION     DAOMAA010001009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_201470_length_1041_cov_2.622718"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1041
                     /locus_tag="PLO59_11445"
     CDS             <1..>1041
                     /locus_tag="PLO59_11445"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HQV01770.1"
                     /translation="LLAGFLRKKNVQLVKCLTQDMYVPADVDFVIEGYVDPNEPFILE
                     GPFGDHTGYYSLADMYPRFHITCITHRKDAIYPTTIVGIPPQEDAWLGKATERIFLAP
                     IKMTAVPEIVDMVLPVEGVFHNLVIVKIKKDYPGQALKVMHSLWGAGQMMFTKMMIVV
                     DGDVNIHNNLEVAQYISANVNPETDFTFSQGPTDVLDHSCSTMAFGGKMGIDATRKTE
                     EELAMVASHYATNTIVNIDSHWLMQQFTEIKGINCQLLQAGVSILFIAVTKNRKNHIK
                     ELAQKLFDIEAMQTVKCVIFLEDIFDINDIADAVWRFSNNMDPKRDHLFIHHQTTSII
                     FDGTRKTKEFDGF"
BASE COUNT          340 a          198 c          200 g          303 t
ORIGIN      
        1 ttacttgccg gttttttacg caaaaaaaat gtgcaattag taaaatgcct cacacaagat
       61 atgtacgtgc ctgccgatgt tgattttgtt atcgaaggat atgttgaccc caacgaaccc
      121 tttattttag aaggcccctt tggcgaccac accggctact attcattagc cgatatgtat
      181 ccgcgttttc atattacctg cataacgcat cgcaaagatg ccatatatcc cactaccatt
      241 gtaggcatac caccacaaga agatgcctgg ttaggtaaag caacagaacg gatattttta
      301 gctcccataa aaatgactgc tgtacccgaa attgttgata tggtgttgcc ggttgaaggc
      361 gtatttcaca atttggttat tgtaaaaatt aaaaaagact atccgggcca ggccttaaaa
      421 gtaatgcata gtttgtgggg tgcaggtcaa atgatgttta caaaaatgat gattgtggtt
      481 gatggcgatg taaatatcca taacaactta gaagtggcac aatacatttc ggccaacgtt
      541 aatcccgaaa ccgattttac attttcgcaa ggcccaaccg atgtgttaga tcatagttgc
      601 agtaccatgg cctttggtgg taaaatgggc attgatgcca cccgcaaaac cgaagaagaa
      661 ttagcaatgg ttgcttcaca ttacgcaaca aacacaattg taaatattga tagccattgg
      721 ttaatgcaac aatttaccga aataaaaggc attaactgtc aattattgca agcaggtgtt
      781 tctattctgt ttatagctgt tacaaaaaac cgcaaaaacc acatcaaaga attggcacaa
      841 aaactatttg atatagaagc aatgcaaacg gttaagtgtg ttatattttt agaagatatt
      901 tttgatataa atgatattgc tgatgcagta tggcgcttta gtaataacat ggacccgaaa
      961 cgcgatcact tgttcattca ccatcaaact acttcaataa tttttgatgg cacacgtaaa
     1021 accaaagaat ttgatggctt t
//
LOCUS       DAOMAA010001010         1039 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_202260_length_1039_cov_2.410569, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010001010 DAOMAA010000000
VERSION     DAOMAA010001010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_202260_length_1039_cov_2.410569"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="PLO59_11450"
     CDS             complement(<1..670)
                     /locus_tag="PLO59_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01771.1"
                     /translation="MLKTKLNSFSMIMGVFFLGILILKPNSSQATHAAGADITYQCLG
                     NNQYRITVAFYRDCFGIPAPTPTTDVVAYSSCFGSISVTLNEISVTNITPACDTVITC
                     VGGANGVEKHVYTGIVTLPGICADFTFYWTLCCRNAAITNLTNAAGYGATFYATLDNT
                     QTPFNSSPVFANDPVIYLFTNQLYHINNGMYDSDGDSLVISMIEPLDGNTTTGALTAL
                     PIPYN"
     gene            867..>1039
                     /locus_tag="PLO59_11455"
     CDS             867..>1039
                     /locus_tag="PLO59_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017260107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HQV01772.1"
                     /translation="MTIKDLIAFFETIAPRTYQESYDNSGLITGNAASEITGVLVSLD
                     CTEAVVEEAIALK"
BASE COUNT          340 a          177 c          211 g          311 t
ORIGIN      
        1 ggttgtatgg aataggcagt gcagttaatg caccggtagt tgtattacca tccaaaggtt
       61 caatcatact tataaccaac gaatcgccat cgctatcgta catgccgtta ttgatgtggt
      121 aaagctgatt ggtaaaaagg taaataaccg gatcgttggc aaatacagga gaactattaa
      181 atggcgtttg cgtattgtct aacgtagcat aaaaagttgc accataacct gctgcattgg
      241 ttaggtttgt aatggctgca ttgcgacagc acaatgtcca gtaaaatgta aaatcggcac
      301 agatgccggg aagggttaca ataccggtat aaacatgttt ttcgacacca ttggcaccgc
      361 caacacaggt aattactgtg tcgcatgcag gtgtaatatt ggttaccgat atttcgttta
      421 aggttaccga aatgcttcca aaacaggatg agtaagctac tacatcggtt gtgggtgtag
      481 gtgccggaat accaaaacaa tcgcgataaa acgccactgt tattcggtat tggttattac
      541 caaggcattg gtaagtaatg tcggcacctg cagcatgagt tgcctgtgat gagttgggtt
      601 ttaaaattag tattcctaaa aagaatacgc ccataatcat agagaaagag tttagttttg
      661 tttttaacat tgcatttaaa gtttaaaaaa ttattaacga tttttattag ctgatttttg
      721 aattatgctt tggtttagca caaaaaaata aaaaattatt aacaaaagca acatccaaca
      781 tatatttctt caaacaaaat tgttaatgct tcttaagcta attagtgtaa attaactaat
      841 attgcataat tcaaccatca taaaaaatga caataaaaga cttaatcgct tttttcgaaa
      901 ccatagcacc tcgcacttat caggaaagtt atgacaacag tggtttaatt accggcaatg
      961 cagcatcaga aattaccggt gtgcttgttt cgttagactg caccgaggcc gttgttgaag
     1021 aggctattgc gttgaaatg
//
LOCUS       DAOMAA010001011         1039 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_202296_length_1039_cov_2.215447, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010001011 DAOMAA010000000
VERSION     DAOMAA010001011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_202296_length_1039_cov_2.215447"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="PLO59_11460"
     CDS             <1..333
                     /locus_tag="PLO59_11460"
                     /inference="COORDINATES: protein motif:HMM:NF024684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HQV01773.1"
                     /translation="RPQAAYPRFDFKLYPTLTFENVDKTVFKNLQLAIDAMAMGGIMP
                     CALNAANEIAVQAFLNDEIGFVQMSEVIAACLDATTNETQPALETLMHTDAQTRIKAS
                     KICKSLGH"
     gene            450..>1039
                     /locus_tag="PLO59_11465"
     CDS             450..>1039
                     /locus_tag="PLO59_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010599419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HQV01774.1"
                     /translation="MDALIMAGQLILALSILVTLHECGHFWAARAFGIKVEKFYLFFD
                     AWDKKLFSFKRGETEYGVGWLPLGGYVKIAGMVDESMDTEALKAEPQPYEFRSKPAWQ
                     RLIVMIGGVTMNLILGVAIFTCILLFAKKEYLPNNAVTKGIVSYKLGDEIGFKTGDKI
                     VAINGETPERFDAIYSTDVLFGSTITVLRNNQKVAI"
BASE COUNT          322 a          194 c          216 g          307 t
ORIGIN      
        1 cggccgcagg ctgcatatcc acggttcgat tttaagcttt acccaacact tacattcgaa
       61 aatgttgata aaacggtttt caaaaattta caattagcca ttgatgcaat ggctatgggc
      121 ggcattatgc cttgtgcttt aaatgcagcc aacgaaattg ccgtgcaagc ttttttaaat
      181 gatgaaattg gttttgtgca aatgtcggaa gtaatagctg cgtgtttgga tgctaccacc
      241 aacgaaacac aacctgcatt agaaacgctg atgcataccg atgcgcaaac acgtattaag
      301 gcaagtaaaa tttgtaaatc gctcgggcat taataaaatg gtaacgcact tacattaaat
      361 atacttccgc aggtagccgt taaacaatat tatattcgcg cgcattttta aaatcaaaca
      421 agtaaaactg cgctcaatta tctaaaaaca tggatgcatt aattatggcc ggccagttaa
      481 tactggcact ttcaatattg gtaaccttgc atgagtgcgg ccacttttgg gctgcccgtg
      541 cttttggcat taaggtagaa aaattttacc tcttttttga tgcctgggat aaaaaactgt
      601 ttagctttaa gcgtggcgaa acggagtatg gcgtgggctg gttgccttta ggcggctatg
      661 taaaaattgc aggcatggtt gacgaaagta tggataccga agctcttaaa gccgaacctc
      721 agccttacga attcagaagc aaaccagcct ggcaacgctt aattgttatg attggtggcg
      781 taactatgaa tttaatttta ggtgttgcca tttttacatg catattattg tttgctaaaa
      841 aagaatatct gccaaataat gccgtaacca aaggcattgt ttcttataaa ctgggcgatg
      901 aaataggttt taaaaccggt gataaaattg tggctattaa tggcgaaacg cccgaacgtt
      961 ttgatgctat ttatagtacc gatgtattat ttggttcaac aataaccgta ctacgcaaca
     1021 accaaaaagt agctattca
//
LOCUS       DAOMAA010001012         1038 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_202576_length_1038_cov_3.162767, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010001012 DAOMAA010000000
VERSION     DAOMAA010001012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_202576_length_1038_cov_3.162767"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(396..>1038)
                     /locus_tag="PLO59_11470"
     CDS             complement(396..>1038)
                     /locus_tag="PLO59_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQV01775.1"
                     /translation="TNKALAAINKKATWAFYIQGDEAVHEKYLDNIKAAMQQHAHNQK
                     VEGLLFKYMHFYGSYDYVGDSRRWYRNEVRIVRPNEALTSYKDAQGFQIKNRPLNVKP
                     VEAYIYHYGWVRPPDYQQKKQRDFNKLWHDDAHVEKKFADIAQFDYTQIDSLAKFTGT
                     HPKVMQQRIERINWEFDFDPTQKKQTLKNKVSGFFEKHFNWRPGEYKNYKLLK"
BASE COUNT          296 a          198 c          191 g          353 t
ORIGIN      
        1 gccgcttata gccaatcata ttgcaacaca aaaaacaact tgcatagcca acacccaagc
       61 cgattattta atatcaaccg atatgggctg cctgcaacac ctgcaaacag caattaaaaa
      121 tgccggccta aaaacagaag taatgcattt ggccgatgta ttggcaaaag ggtggtaagt
      181 ttttttaacc cttgttgcaa agttttatgg ttaatgcata gcgcatgtag ccactgttta
      241 taaattaatt tttagtaccg tatttgggta aagttggaca agtcgagcgt ctataaagct
      301 ataacagtaa ttattatctg ccgcacaaat tttgtttagc tcaaacattg ttgcagctta
      361 taagcttaaa atttgtatgg tctatagtca attaactatt ttaaaagctt atagtttttg
      421 tattcgcccg ggcgccagtt aaagtgtttt tcgaaaaaac cggacacctt gtttttaagc
      481 gtttgttttt tttgtgtagg gtcaaaatca aattcccaat ttattctttc aattcgttgc
      541 tgcataacct tagggtgtgt gccggtaaat tttgccaatg aatcaatttg tgtgtaatca
      601 aactgcgcta tgtcggcaaa ttttttttca acgtgggcat catcgtgcca taacttatta
      661 aagtcgcgtt gttttttttg ctgatagtcg ggtgggcgca cccaaccgta atgatatata
      721 tatgcctcaa ccggctttac atttaaaggg cggtttttaa tctgaaaacc ttgtgcatct
      781 ttataggaag tgagtgcttc attaggtcta acaatacgca cttcgtttct ataccaacgc
      841 ctgctgtcgc ctacataatc atacgagccg taaaaatgca tatacttaaa taacaagcct
      901 tctacctttt ggttatgcgc atgctgttgc atggcagctt ttatattatc taaatatttt
      961 tcatgtacgg cctcgtcacc ctgtatataa aaagcccaag ttgctttttt attaatggca
     1021 gctaaagctt tattggtt
//
LOCUS       DAOMAA010001013         1038 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_202619_length_1038_cov_2.606307, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010001013 DAOMAA010000000
VERSION     DAOMAA010001013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_202619_length_1038_cov_2.606307"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="PLO59_11475"
     CDS             complement(<1..250)
                     /locus_tag="PLO59_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01776.1"
                     /translation="MTDYIIIIALIIVTCLIFGFIIYRIKKQYRATLTSILVQINHLR
                     KGHFDKKISGSVADDLIIEINHSLHEWEKEQANEFEKLK"
     gene            459..875
                     /locus_tag="PLO59_11480"
     CDS             459..875
                     /locus_tag="PLO59_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HQV01777.1"
                     /translation="MLSKKCKYALKALEYLAKQKNATPVLIAEISEKQKIPKKFLEVI
                     LLELKHDGIVQSKMGKHGGYYLIKKPADINIGHIIRLIDGPVALLPCVSYKYYQPCDD
                     CEDEATCGLHHVLAKIREANNKILGNVSLAGMINFK"
BASE COUNT          357 a          168 c          188 g          325 t
ORIGIN      
        1 tttttagttt ttcgaattca ttggcttgct ctttttccca ctcatgtaat gaatgattaa
       61 tttcaataat caaatcgtca gctaccgaac cggatatttt cttatcgaaa tggcctttac
      121 gtaaatgatt aatctgaaca aggatggatg taagtgtggc gcgatattgt tttttaattc
      181 gataaattat aaagccaaaa atcaaacacg ttacaattat taaagcaata atgattatat
      241 aatcagtcat ttagttgaaa caccggttta aaattaatta ggcaaggtaa aggctttaca
      301 gattactaaa actagggtta ccaaatttaa tgcaccacta acaaataata attcggccaa
      361 ccaaattatt gtacaccaac tgttacaatt tcaaaatggt ttttacattt gaccggtatg
      421 ttacccttta accactttga gggtttatct gatttactat gctttctaaa aaatgtaaat
      481 acgctttaaa ggccttagag tatctggcaa aacaaaaaaa tgccacaccg gttttaattg
      541 cagagataag cgagaagcaa aaaatcccca aaaaattttt agaagttatt ttgcttgaat
      601 taaagcacga tggcattgta caaagcaaaa tgggtaaaca tggcggttat tacttaataa
      661 aaaagccggc cgatattaat attggccata ttatcaggtt aattgacggg cctgtggctt
      721 tgttgccctg cgtttcgtat aagtattatc agccctgcga tgattgtgaa gacgaagcaa
      781 cttgtgggct acatcatgtt ttagccaaaa tacgcgaagc caacaataaa atactgggta
      841 atgtgagttt agcggggatg attaatttta aataatcata aacaaagccg ctggaaacta
      901 cgattattaa aaaaattgtg ctttttttta ataatgcttt tgcacaaaag aaaaaaatat
      961 atttttgcgg cctcattaag cgaaagtagc tcagttggta gagcgcaacc ttgccaaggt
     1021 tgaggtcgcg agttcgag
//
LOCUS       DAOMAA010001014         1038 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_202755_length_1038_cov_1.836216, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010001014 DAOMAA010000000
VERSION     DAOMAA010001014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_202755_length_1038_cov_1.836216"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /locus_tag="PLO59_11485"
     CDS             complement(<1..1023)
                     /locus_tag="PLO59_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01778.1"
                     /translation="MLAGAVVGSNIENASIYYNPGTLGFADSIKVTVSSGLYEFTAQR
                     FTNGVNSGQNLIATDVKRFPLSIFPTFKLKNNTTLGFIYVPRLYSNTIVNDVQQNRVD
                     VLQNNKPVPFYTSFDISNYESEWWYGVSFSKKLDKRSGIGITGFVSYRNQNFRYQYAA
                     DAWVDTINNTYMNTLMQRQIKYYCYKTIFKIGYFNKHDKYAWGCAATIPAFAVYSNGK
                     TSTRFWFNNFIEADSTSEQTFIAEQQINLKADFKYPITISAGGQLVKGKCTYYLTAES
                     FFEIAPYDVLEINNRIIEINGVTINANKIAGAQTASKFITNVAVAMQYRYKPKQTIAI
                     GLATDFN"
BASE COUNT          308 a          193 c          190 g          347 t
ORIGIN      
        1 attaaagtca gtggctaagc caattgcaat ggtttgtttg ggtttgtaac gatattgcat
       61 ggccactgct acgttggtta taaatttaga agcggtttgc gctccggcaa ttttattggc
      121 atttattgtt acgccattta tttcaattat acggttatta atttccaata cgtcataagg
      181 tgctatttca aaaaacgatt ctgcggttag gtaataggta cacttaccct ttactaattg
      241 cccaccggca cttatagtaa ttggatattt aaaatcggct tttaaattaa tttgttgttc
      301 ggcaataaaa gtttgttccg aagtactgtc ggcctcaata aaattattaa accaaaatcg
      361 tgtgcttgtt ttgccgtttg aataaacagc aaatgcaggt atagtagccg cacatcccca
      421 agcatattta tcatgcttat taaaatatcc tattttgaaa atagttttgt agcagtaata
      481 ttttatctgc cgttgcatga gtgtgttcat gtacgtattg ttgatggtat ctacccaggc
      541 atcggctgca tactggtacc ggaaattctg attacggtaa cttacaaaac ctgtaatacc
      601 aataccagat cgcttatcta gtttcttaga aaatgacaca ccataccacc actcgctttc
      661 ataattcgaa atatcgaatg aagtataaaa aggaaccggt ttgttgtttt gcaaaacatc
      721 aacccggttt tgttgtacat catttacaat ggtgttacta tacaacctgg gtacatatat
      781 aaaacccagc gttgtattgt ttttaagttt aaatgttgga aaaatagaaa gcgggaaacg
      841 tttaacatct gtagctatta aattttgacc actatttaca ccatttgtaa atcgctgtgc
      901 cgtaaactca tacaagcctg atgatacggt tacttttatt gagtcggcaa aacctaacgt
      961 acccgggttg tagtaaatag atgcattttc gatattgctg ccaaccacgg cacctgccag
     1021 catgctgctt aagttgcc
//
LOCUS       DAOMAA010001015         1037 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_202967_length_1037_cov_2.802444, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010001015 DAOMAA010000000
VERSION     DAOMAA010001015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_202967_length_1037_cov_2.802444"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="PLO59_11490"
     CDS             complement(<1..313)
                     /locus_tag="PLO59_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01779.1"
                     /translation="MKKISTTSRILMAFASLAIIAAYFFPVWRIDLFAPQYPEGLVMK
                     IWLNKLTGDVDVINGLNHYIGMKKISVEMFPELTFLVYLVAGFIAFGLAVSITGSSKL
                     LL"
     gene            complement(370..936)
                     /locus_tag="PLO59_11495"
     CDS             complement(370..936)
                     /locus_tag="PLO59_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019991170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HQV01780.1"
                     /translation="MKKINLTLTSLLLCGLVACNNSNNQTASTETTPTEETTAGGQEA
                     VLDNDSEKDIVKIAVGSKDHTTLVAALKQAEYVNDLSNAGPFTVFAPTNDAFNKLPAG
                     TVDDLMKPENKDKLTDILEYHVAVGGYKQDMLTDGQTINQANLKNITINIKDGKMMVN
                     NVANVVATIPAANGIIYVIDAVLLPPAN"
BASE COUNT          312 a          182 c          193 g          350 t
ORIGIN      
        1 caagcagtaa tttggagctg cctgtaattg aaacggctaa accaaatgcg ataaagccgg
       61 caaccagata aactaaaaaa gtaagctcag gaaacatctc cacactaatt tttttcatgc
      121 ctatataatg gttaagacca ttaataacat ctacatcacc tgtaagttta ttaagccaaa
      181 ttttcatcac caatccttcg ggatattgag gtgcaaacaa atcaatacgc cataccggaa
      241 aaaagtaagc cgcaattata gctaacgatg caaatgccat tagtatgcgc gatgtggtgc
      301 ttattttttt cataagaagt ttaattaata gattttaata aaaggggtaa taagtatttt
      361 taattcgtat taattggctg gtggcaatag cacagcatca ataacataaa taattccgtt
      421 ggctgccggt atggttgcaa caacattggc aacgttatta accatcatct ttccgtcttt
      481 aatattaata gttatgtttt taagatttgc ctggtttatt gtttggccat ctgtcagcat
      541 atcttgttta tagccaccca cggcaacatg gtactcgagt atatcagtaa gcttatcttt
      601 attttccggt ttcatcaaat cgtcaacggt acctgcaggt aatttattaa aggcatcgtt
      661 tgttggggca aatacagtaa acgggcctgc attggataaa tcatttacat actcagcttg
      721 ttttaaagcg gctaccaatg ttgtgtgatc tttagatccc acagcaattt ttacaatgtc
      781 tttttctgaa tcgttatcaa gcacggcttc ctggccgcct gcagttgttt cttcagtagg
      841 tgttgtttcg gttgaagcag tttgattgtt gctgttattg catgcaacca aaccacaaag
      901 gagtaatgat gtaagggtta aattaatttt tttcataagg tttgtttttt aaagcaatgg
      961 agaaaattat ttgcactgaa catttaattt tctccattgc aatttttgat agaattattt
     1021 agcggattcg ggggctt
//
LOCUS       DAOMAA010001016         1036 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_203313_length_1036_cov_3.395515, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010001016 DAOMAA010000000
VERSION     DAOMAA010001016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_203313_length_1036_cov_3.395515"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="PLO59_11500"
     CDS             complement(<1..750)
                     /locus_tag="PLO59_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01781.1"
                     /translation="MDVKNASGNIIFCCTSVSSFVAKDIQILTHAGYTVTPLLFIWHP
                     KWRLPVTFLKQFCQLFYYLFTNRMIIVQFGGMHAFLPVIMAKIWRKPSLVILGGTDCA
                     KFPSIAYGNFNKKILGWFTAQSVKHAMHLAPKHASLMWFDYTYDSSDYPAQGIQCFAL
                     NLKTPFTIIENGYDATVFKRVCDKKQHTFLTVAAGIELPNTMALKGIDLILKVAPHFS
                     HCTFTIVGVPTDFNLPVQLKNVVVLPQQNAAQ"
     gene            complement(804..>1036)
                     /locus_tag="PLO59_11505"
     CDS             complement(804..>1036)
                     /locus_tag="PLO59_11505"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HQV01782.1"
                     /translation="TPNDDGLNDVFSITGSVCDTFEIMIINRWGFQVAYLNKNNQVWN
                     GKYKNADVPEGIYFYKAKTNGKEFTGTISLLR"
BASE COUNT          340 a          186 c          185 g          325 t
ORIGIN      
        1 ttgtgcagca ttttgttgtg gcaaaacgac tacatttttt aattgtaccg gtaaattaaa
       61 atcagttggc acgccaacaa tggtaaatgt gcaatggcta aaatgcgggg ctacttttaa
      121 aattaaatca atacctttta atgccatggt attgggcaat tctataccgg cagcaacagt
      181 taaaaatgta tgttgttttt tatcacaaac tcttttaaaa acagttgcat catatccgtt
      241 ttcgataatt gtaaaaggtg tttttaaatt gagtgcaaaa cactgtatgc cttgtgccgg
      301 atagtcgctg ctatcgtatg tataatcaaa ccacatcagc gatgcatgct tgggtgccaa
      361 atgcattgca tgctttaccg attgtgctgt aaaccatcct aaaatttttt tattaaagtt
      421 accataagcg attgatggaa actttgcaca atcggtgcca cctaaaatta ccagcgatgg
      481 cttgcgccaa attttagcca taattaccgg caaaaatgca tgcatgccac caaattgaac
      541 aattatcata cggtttgtaa acaagtagta aaacaactga caaaattgtt ttaaaaacgt
      601 aaccggtaaa cgccatttgg gatgccaaat aaatagcagc ggtgtaacag tataaccggc
      661 atgggttaat atttgtatgt ctttagcaac aaaggatgaa accgaagtac aacaaaaaat
      721 aatattgccc gaagcatttt ttacatccat attttagttt gatagccaca aatgaaaata
      781 cattgatttg ttttgcaaaa gcatcagcgt aataatgaga tggtgccggt aaattctttc
      841 ccattggttt tggctttata aaagtaaatc ccttcaggta catcggcatt tttgtattta
      901 ccattccata cttgattgtt tttgttgagg tatgcaacct gaaagcccca acggttaatt
      961 atcattattt caaaggtatc gcaaacagaa ccggttatgc taaaaacatc atttaaccca
     1021 tcgtcattag gtgtaa
//
LOCUS       DAOMAA010001017         1035 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_203746_length_1035_cov_3.531633, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010001017 DAOMAA010000000
VERSION     DAOMAA010001017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_203746_length_1035_cov_3.531633"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="PLO59_11510"
     CDS             <1..911
                     /locus_tag="PLO59_11510"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQV01783.1"
                     /translation="TTGDKYLYCYTTNPNGAATDGNTANNRLSRRLTIKATNAAMPVV
                     QSFSATTFPPSQWTNKRYDCNTGWGRNTTAYFSAPASMYFNNFGQTPGNAKRIFDLYT
                     QPINLSTQPNPTLSFNVAYAQRNASTLDTLEILISTDCGYTYTSVYKKWGTALETASA
                     TTSAFIPTSTQWRNEVINLTAYATATNAIFIVRNITKGGNNVYIDDITVDQLQGIELN
                     DVSDLVTVFPNPTSGLFQINIPASNQVSYVSVVSALGETVIHKNTIEAGLTSTTFDLS
                     NFVNGVYFVTIENENGKAVKKVSLNK"
BASE COUNT          351 a          216 c          175 g          293 t
ORIGIN      
        1 caaccacagg cgataaatat ttatactgct atactaccaa tcctaatggt gctgctaccg
       61 atggaaatac tgccaataac cgcttgtcgc gcagattaac tattaaagca accaatgcag
      121 ccatgccagt ggttcaaagt ttttcagcga caacatttcc gccatcgcaa tggactaata
      181 aacgttacga ttgtaataca gggtggggac gcaatacaac agcctatttc tcagcaccgg
      241 caagtatgta ctttaataat tttgggcaaa cacctggcaa cgcaaagcgc attttcgatt
      301 tatacacaca acccattaac ctaagtacac agcccaatcc aaccctcagc tttaatgttg
      361 cttatgcaca acgcaatgca agtacacttg atacattgga gatattaatc agtaccgatt
      421 gcggatacac ttacacatcg gtatataaaa aatggggcac tgcattagaa actgcatcag
      481 caaccacatc ggcatttatt ccaacatcaa cacaatggcg taacgaagta attaatctta
      541 cagcctatgc aacggcaacc aatgctattt tcatcgttag aaatattacc aaaggcggca
      601 ataatgttta tattgatgat attacggttg atcaacttca gggtattgaa ttaaacgatg
      661 tttctgactt ggtaacagtt tttcctaacc ctacatcagg tttgtttcag ataaatatac
      721 cggcatcaaa tcaggtaagt tatgtttcag ttgtttcagc cctgggcgaa acggttatcc
      781 acaaaaatac cattgaagcc ggtttaacct caacaacctt cgatttatca aattttgtaa
      841 atggagttta ctttgtaacc attgaaaacg aaaatggtaa agcggtaaaa aaagtatcat
      901 taaataaata attaaataac agtaagcttc atatcgttca aatgaaaaaa acagcatttc
      961 ttatttcgtt ttttgcctgc agtcttattg ctaatgcaca acaagtgcct gcaaatttat
     1021 gggcagatat tgaaa
//
LOCUS       DAOMAA010001018         1035 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_203752_length_1035_cov_3.402041, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010001018 DAOMAA010000000
VERSION     DAOMAA010001018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_203752_length_1035_cov_3.402041"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(221..>1035)
                     /locus_tag="PLO59_11515"
     CDS             complement(221..>1035)
                     /locus_tag="PLO59_11515"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF012991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HQV01784.1"
                     /translation="KHVEKVLEYGIDVVWVGARTTVNPFFVQEIAQALKGVKIPVLVK
                     NPLHPDLKLWLGAIERIKKSGIDDITAVHRGFYTYEKTAYRNTPRWELLLELKLLQPD
                     LQVICDISHIAGNTVLLAQVAQQGLDLNVDGLMVETHFNPQQAKSDAQQQITPEQLHL
                     LLSTLKYRQPHFKDVTLKNALQQLRLQIDGIDAEILKLLSQRMELVTDIAALKDAAQV
                     SIFQIERWRSILDQALVQADTLGLHADFVKNLFIQIHDESIRIQSAILNKNC"
BASE COUNT          295 a          201 c          191 g          348 t
ORIGIN      
        1 accttgttgt gtggttttgt taattaccat caggttattg atgcttattt tactatcgcc
       61 atcgtaaacc actccggcaa tttgtatgcg cggtgtttca tcagtttgcg ctgttgcaag
      121 caaaggaaaa agtaaaaagt aaacggaagt cttaattttt gacaagtgca tatttcaaat
      181 taagacaggt cattattttt aagcaatggg ttaaagtcat ctaacagttt ttattaagta
      241 tggccgattg aatacggata ctttcatcat gtatttgaat aaataaattc tttacaaaat
      301 cggcatgcag gcctaatgta tcagcttgca caagtgcctg atctaatatg ctgcgccaac
      361 gctctatctg aaaaatactt acctgtgctg catctttcaa agctgcaata tctgttacca
      421 actccatacg ttggcttagt aattttaaaa tttcggcatc aatgccatca atttgaagcc
      481 gcaattgttg caaggcattt tttagcgtta catctttaaa atgtggttgt ctgtatttaa
      541 gtgtgcttaa taaaaggtgt aactgctccg gggtaatttg ttgctgggca tcgcttttag
      601 cttgttgtgg gttaaaatgt gtttctacca ttaaaccatc aacatttaaa tccaatcctt
      661 gttgtgcaac ttgggctaac aaaaccgtat tacctgcaat atggcttatg tcacaaataa
      721 cctgcaagtc gggttgcagt aactttaatt caagtagtaa ttcccaccgg ggtgtattac
      781 gatacgctgt tttttcatag gtgtaaaatc ctcgatgcac agccgttata tcatcaatgc
      841 cgcttttttt aattcgctct atggcaccta accaaagttt aaggtcgggg tgcaaagggt
      901 tttttaccaa tacaggaatt ttaacgccct ttaaggcctg tgcaatttcc tgtacaaaaa
      961 acgggtttac cgttgtgcgc gcaccaaccc aaacaacatc aataccatat tccaatactt
     1021 tttcaacatg ttttg
//
LOCUS       DAOMAA010001019         1033 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_204688_length_1033_cov_2.143149, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010001019 DAOMAA010000000
VERSION     DAOMAA010001019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_204688_length_1033_cov_2.143149"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1033
                     /locus_tag="PLO59_11520"
     CDS             <1..>1033
                     /locus_tag="PLO59_11520"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HQV01785.1"
                     /translation="LNDLWEYDPISNSWTQRANFPGVPRYDACGFSIGQKGYVGFGYP
                     PILKDVFAYDPTTNSWSQIAAFPGSSRQSLSTFEIDGLGYFSCGSNSGTVSYNDLWCY
                     NPSTNSWSQKANFPGAPRYATNGFSLSGKGIIGGGCNNSQSFNDYYIYNPTSDSWCQL
                     LSFPAQARYAGDAFSIGQKGYTGLGRDKLHIYYYDFWELTYIPATFTFQDTACSLDIG
                     FNAQAINPNAFNWSFGDGGISTLSNPSHTYASSGSYNVQLITYYNCGSDTVTQQVQVT
                     NTTTAALALNKTCNEVSFTNHSINATNYLWSFGDGNTSTQTTPVHHYTDTGTYAISLI
                     AYNNNCTDS"
BASE COUNT          306 a          207 c          198 g          322 t
ORIGIN      
        1 ctttaaatga tttgtgggag tacgatccaa tcagtaatag ttggacacag agagcgaatt
       61 ttccgggcgt gccgcgttac gatgcatgtg gcttttctat aggtcaaaaa ggctatgttg
      121 gttttggata tccacccata cttaaagatg tgtttgcata tgatccaaca acaaattcct
      181 ggtcacaaat tgcagcattt ccagggtcat cacgtcaaag tctttctaca tttgaaatag
      241 atgggttagg atatttttca tgtgggtcaa atagcggaac tgtttcctat aatgatttat
      301 ggtgttataa tccttcaaca aattcttggt cacaaaaagc taattttccg ggagctcccc
      361 gttatgcaac taatggattt agcttaagcg gcaaaggcat tataggtggt ggttgcaata
      421 attcccaatc gtttaatgat tactacatct ataatcctac ttcagatagt tggtgtcagt
      481 tgttatcatt tccagctcaa gcgcgttatg ctggagatgc cttttcaatc ggtcaaaaag
      541 gatatactgg tttaggacgc gacaagttac atatttatta ctatgacttt tgggaactta
      601 cctacatacc ggcaacattc acttttcagg atacagcttg cagcttagat attggtttta
      661 atgcacaggc tatcaatccc aatgcattta attggtcgtt tggcgatggt ggtataagta
      721 ctttatcaaa tccaagtcat acctatgcaa gcagcggcag ctataatgtg cagcttataa
      781 cttattacaa ctgtggcagc gataccgtta cacaacaggt acaggtaacc aataccacaa
      841 cagctgcttt agccctaaat aaaacctgca acgaagttag ttttaccaat cattcaatta
      901 atgcaacaaa ttatctttgg agttttggcg atggtaatac ctccacacaa accacacctg
      961 tgcaccatta taccgatacc ggtacttatg ctatttcact cattgcatac aacaacaatt
     1021 gtacggatag tat
//
LOCUS       DAOMAA010001020         1032 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_204960_length_1032_cov_3.139202, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010001020 DAOMAA010000000
VERSION     DAOMAA010001020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_204960_length_1032_cov_3.139202"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="PLO59_11525"
     CDS             complement(<1..588)
                     /locus_tag="PLO59_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQV01786.1"
                     /translation="MFKIIKYVMVDILRNKIVLAYTIFLLAISFSIFNIEDSSSKGLL
                     SMLNVVLIIVPLVSLIFSTIYMYNAVEFIELLLSQPIQRKNLLLSIYTGISLSMIIAF
                     VIGAGIPLLLLEGSPTAIIMLLVGMLLTAIFVSLAVLGAVITRDKAKGIGIAIMMWLY
                     FALLYDGLVLLIMFQFSDYPLESAVVIFCSLNPVDL"
     gene            complement(635..>1032)
                     /locus_tag="PLO59_11530"
     CDS             complement(635..>1032)
                     /locus_tag="PLO59_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015806832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQV01787.1"
                     /translation="LIEAFELEKMFHKRMRTLSGGTRQKVSASLAFLFNPQVLILDEP
                     TAGLDPVATEILKEKIRKEKQQGKLILITSHILSDLDDLVSEIVYFNEGHLLFHKNIT
                     LLRNETGEEKLSRAIAYIMKSNLMQKTIN"
BASE COUNT          347 a          186 c          162 g          337 t
ORIGIN      
        1 taagtcaaca ggatttaatg aacaaaaaat taccacagcg ctttctaaag ggtaatcgct
       61 aaactgaaac ataattaaaa gtacaaggcc atcatacaat aatgcgaagt acagccacat
      121 cataattgca atgccaatac ctttagcttt atcgcgggta ataacagcgc ctaaaacagc
      181 caacgaaaca aaaatggctg ttaaaagcat gcccactaac aacatgataa ttgctgttgg
      241 cgagccttca agtaacagca atggaatgcc tgcgccaatc acaaaagcaa ttatcatcga
      301 aaggctgatg cctgtataaa tgcttaataa taaattttta cgctgtattg gctgcgacaa
      361 taacagttcg ataaactcaa cggcattgta catgtaaatg gtactaaaaa ttaaacttac
      421 caagggtaca attatcaata caacatttag catactcaat aatccttttg acgaactatc
      481 ttcaatgtta aaaatgctga atgatatggc aagcaaaaaa atggtatagg ccaatacaat
      541 tttgtttcgc aatatatcaa ccattacgta ttttataatt ttaaacatat cgtatcctta
      601 cttatccaag tttttaagtt tcgtattttt agttttagtt gatggttttt tgcattaagt
      661 ttgatttcat aatataagca atggcacgcg atagtttttc ttcgcctgtt tcgtttctga
      721 gtaatgttat gtttttatga aacaataaat gtccttcgtt gaaataaaca atttccgaaa
      781 ccaagtcgtc caaatcgctt aaaatatgtg atgtaattaa tatgagtttg ccttgctgct
      841 tttccttcct tattttttct tttaaaattt cagtagctac ggggtcgagt cctgcagttg
      901 gttcgtctaa aatcagtacc tgcggattaa ataaaaatgc aagcgatgca ctaacttttt
      961 gcctggtgcc acctgaaagt gttcgcattc ttttatgaaa cattttttct aattcaaatg
     1021 cttcaattaa tt
//
LOCUS       DAOMAA010001021         1030 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_205757_length_1030_cov_3.258462, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010001021 DAOMAA010000000
VERSION     DAOMAA010001021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_205757_length_1030_cov_3.258462"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="PLO59_11535"
     CDS             complement(<1..400)
                     /locus_tag="PLO59_11535"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HQV01788.1"
                     /translation="MQTSFSNLVAIKYPIIMAPMFLVSNEAMIQAAMQNGILGTFPTL
                     NYRNEGALAKLLDNLNHFKKSGCTGNYGVNLIVQQSNPLYENHLAICVAKQVPVYITS
                     LGNPKKVIEQANSYGAKVFCDVTNVMHAQKA"
     gene            517..>1030
                     /locus_tag="PLO59_11540"
     CDS             517..>1030
                     /locus_tag="PLO59_11540"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQV01789.1"
                     /translation="MTNVTRIFDILPYQKLKCYNNVALAGKENDQWITYSTDDFLNTA
                     NLVSYALMAMGISKDDKIATISNNRPQWNMFDFGILQVGAVQVAIYPTISENDYKFIL
                     TDADVKLIMVSDADLANKMKNIISQLKPGIEIFTFDKVPGFKHWSDLLDLGKSNVQTD
                     KLQQLKNAVNE"
BASE COUNT          355 a          174 c          176 g          325 t
ORIGIN      
        1 aagctttctg cgcatgcata acattggtta catcacaaaa aaccttagcg ccataactat
       61 tggcttgttc aataactttt ttaggatttc ctaacgatgt aatatataca ggtacttgtt
      121 tggcaacaca tattgctaaa tgattttcat acaaagggtt cgattgctga acaattaaat
      181 taacgccata attacctgtg cagcctgatt ttttaaagtg atttaagttg tcgagcaact
      241 tcgctaatgc accttcattt cgatagttta aggtagggaa agtgcccaat atgccatttt
      301 gcatggcagc ctgtatcatc gcttcgttac tcactaaaaa catgggtgcc attattattg
      361 ggtatttaat tgctactaaa ttgctaaagg atgtttgcat atatttatat tacatttcaa
      421 atttgtagtc acacattttc ttttatgcca aaaaggattt tatttgttca tttcgtaaca
      481 ttacttgctt ttatcatatt acacaaaaaa taaaccatga ctaacgtaac acgaatattc
      541 gatatactgc cataccaaaa attaaagtgc tacaataatg tagcattagc cggtaaagaa
      601 aacgatcaat ggataactta ttcgactgat gattttttaa acacggcaaa tttggttagc
      661 tatgcgttaa tggcaatggg tatcagcaaa gatgataaga tagccaccat aagtaataac
      721 cgaccacaat ggaacatgtt tgattttgga attttgcaag tgggcgcagt gcaagtagcc
      781 atctatccaa ccattagcga aaacgactac aaatttattc ttaccgatgc cgatgtaaaa
      841 ttaataatgg taagcgatgc tgatttggct aacaagatga aaaacattat tagccaacta
      901 aagccgggca tcgaaatttt tacctttgat aaagtacccg gttttaaaca ttggagcgat
      961 ttgttagact tgggaaaaag taatgtacaa acagataaat tacagcaatt aaaaaatgca
     1021 gtaaatgaaa
//
LOCUS       DAOMAA010001022         1030 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_205797_length_1030_cov_2.775385, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010001022 DAOMAA010000000
VERSION     DAOMAA010001022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_205797_length_1030_cov_2.775385"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..118
                     /locus_tag="PLO59_11545"
     CDS             <1..118
                     /locus_tag="PLO59_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015753856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQV01790.1"
                     /translation="VKEYHVERLMRDAKITQIYEGTSEVQKIVISRSLLSDK"
     gene            135..923
                     /locus_tag="PLO59_11550"
     CDS             135..923
                     /locus_tag="PLO59_11550"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HQV01791.1"
                     /translation="MKFFNSLSLALISIVFLIMAGCIPQKKIVYFQDKTEGKSKDKTS
                     LATPIRPDMIIYKKDIVNVLVYTENPDALPGIGSKLEDRTMDNRPAYERGFTVEDNGD
                     LVLPLIGPVPVAGLTVDQAKQEISKRYKKYVANPVVVVKKLSFKISVLGEVAKPGMYF
                     INYESISLLEALSLAGDLTKYGSRTDVKVIRVIDGRQEEFVIDLTDNKTLYSKLFYLK
                     QDDVIYVRQLKNKRWADAAPQVSIVTSVTTVLIALGTLLIVRAK"
BASE COUNT          352 a          182 c          203 g          293 t
ORIGIN      
        1 tgtaaaagaa taccatgtag aacgcctgat gcgtgatgca aaaattacac agatatatga
       61 gggcaccagt gaggtgcaaa aaattgtaat cagtcgatcg ttattgtctg acaagtaacc
      121 acattaaaaa atatatgaag tttttcaact ccctatcgtt ggcgctcatc agcattgtct
      181 ttttaataat ggcaggctgt atccctcaaa aaaagatagt ttattttcaa gataagactg
      241 aaggcaaaag caaagacaaa accagtttgg ctacgcctat caggcccgat atgattatct
      301 ataaaaaaga tatcgtaaac gttttggttt ataccgaaaa ccctgacgcc ttacccggca
      361 ttggttcaaa gttagaagat agaaccatgg ataaccgccc tgcttacgag cgcggtttta
      421 cagttgaaga taatggcgat ttggtactgc cactcatcgg cccggtgcca gttgcgggtt
      481 taacggttga tcaggcaaaa caagaaatat caaaacgata caaaaagtat gtagccaatc
      541 cagtagtggt ggtaaaaaaa ctaagcttta aaatatccgt tttaggcgaa gtggcaaaac
      601 ccggtatgta ctttattaac tacgaatcta tatcactgtt agaggcgcta tcattggccg
      661 gtgatttaac caaatacggt agtcgtaccg atgtaaaagt aattcgtgtt attgatggcc
      721 gtcaagaaga atttgttatt gaccttaccg ataataaaac actttattcg aagttgtttt
      781 atttgaaaca agatgatgta atatatgtgc gccaattaaa aaacaagcgt tgggccgatg
      841 ctgcaccaca agttagtata gttacctcag ttacaactgt acttattgca ctgggtaccc
      901 ttttaatagt acgtgccaaa taaccattta agtttagata tattatattt taagatacaa
      961 caaaatgcca acaaatccgg taattaataa aagagttagt aatgatgagt tagacctggg
     1021 caaaattttt
//
LOCUS       DAOMAA010001023         1029 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_206184_length_1029_cov_3.226899, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010001023 DAOMAA010000000
VERSION     DAOMAA010001023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_206184_length_1029_cov_3.226899"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1029)
                     /gene="rpoN"
                     /locus_tag="PLO59_11555"
     CDS             complement(<1..>1029)
                     /gene="rpoN"
                     /locus_tag="PLO59_11555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HQV01792.1"
                     /translation="SPDYKYSANNSSKDDETYEAPVTVAATFQDQLLAQLGLLPINER
                     DLRIAEHLIGSIDDDGYIRRDLAAISNDLAFTQNLDVSVNDIERILKIVQTFDPAGVG
                     ARNLQECLLLQLQRNDNGSEEYKHAQLIISKYMEEFSRKHYDKIAARCGLDDAALKEV
                     INLVLKLNPKPGNSGYDGPRSVQHIVPDFIVVNNEGVLELSLNSKNMPDLRLSKTYVE
                     MLQTYGKEKNKNRGNREALQFVKQKIDSAKWFMDAINQRNETLLGTMSAIMNYQYEFF
                     LEGDEKLLKPMILKDIADIVGLDISTVSRVANSKYVQTQHSTFLLKRLFSEAMSTESG
                     EEVSSREV"
BASE COUNT          268 a          233 c          195 g          333 t
ORIGIN      
        1 tcacctcgcg gctcgatacc tcctcgccac tttcggttga catggcctcg ctaaacaatc
       61 gtttaagcaa aaaagtacta tgctgggttt gcacatattt actgtttgcc acgcgcgata
      121 cggtgcttat atccaaccca acaatatcgg caatgtcctt taaaatcatg ggctttaaca
      181 gcttttcatc gccttctaaa aaaaactcat actggtaatt cattatggcc gacatggtgc
      241 ctaataatgt ttcgttacgc tgattaatag catccataaa ccacttagcc gaatcaattt
      301 tttgttttac aaactgcaag gcttcgcggt taccgcggtt cttatttttt tctttgccgt
      361 aggtttgaag catttcgaca taggttttgc ttaaccttaa atcgggcatg tttttgctgt
      421 ttaatgatag ctctaacaca ccctcattat ttactactat aaaatcaggc actatgtgtt
      481 gtacactcct tggtccatcg tagcccgagt tgccgggttt gggattaagt ttaagtacca
      541 ggttgattac ttcttttaat gctgcatcat ctaaaccaca acgcgcagca attttatcat
      601 aatgcttacg cgaaaattct tccatgtact tactgatgat tagttgtgca tgcttatatt
      661 cttccgaccc gttatcgttg cgttgtaact gcaacaacag gcactcttgc aaattacgcg
      721 cacccacacc ggcagggtca aaggtttgca caatttttag tatgcgttcg atgtcgttaa
      781 cagaaacatc taagttttgt gtaaatgcca aatcgttcga aatggctgcc aaatctctgc
      841 gtatgtaacc atcatcatca atgctgccaa ttaaatgttc ggcaatgcgc aaatcacgtt
      901 cattaatggg cagcaggcct aattgtgcaa gtaattgatc ctgaaaagtt gcagcaacgg
      961 ttacgggcgc ttcataggtt tcgtcatctt tactgctatt gtttgcacta tacttgtaat
     1021 cgggcgagt
//
LOCUS       DAOMAA010001024         1027 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_207063_length_1027_cov_2.613169, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010001024 DAOMAA010000000
VERSION     DAOMAA010001024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_207063_length_1027_cov_2.613169"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1027)
                     /locus_tag="PLO59_11560"
     CDS             complement(<1..>1027)
                     /locus_tag="PLO59_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HQV01793.1"
                     /translation="EVIAGAFVSTEDGTGIVHIAPTFGADDARVAKQAGIEAILVTDD
                     AGKPMPLVDKQGRFVKHVTDFASEYVKQEYLTENELEAAKKQQGRENYLSVDERIAIK
                     LKTENKAFKVARYEHSYPHCWRTDKPVLYYPLDSWFIRTTAYKDQMLALNKTINWKPE
                     STGTGRFGNWLENLVDWNLSRSRFWGIPLPIWRNDDGEEKCIGSIDELRIEMKKAIDA
                     GFIDEKHLEFIDPNSPQFDLHRPYVDHIVLVSDTGKKLWREPDLIDVWFDSGAMPYAQ
                     WHYMGKKDTAPFGKEPAFPADFIAEGVDQTRGWFFTLHAIATMLFDSVAFKNVVSNGL
                     VLDKNGNK"
BASE COUNT          272 a          233 c          212 g          310 t
ORIGIN      
        1 tttgttgccg tttttatcta ataccaaacc attactaact acatttttaa aagccacgct
       61 atcaaacagc atggtggcaa tagcatgcag tgtaaaaaac cagccacgtg tttggtccac
      121 gccttcggca ataaaatctg ccggaaatgc cggttcctta ccaaatgggg cggtgtcttt
      181 tttgcccatg taatgccatt gggcataagg catggcaccg ctatcaaacc atacatcaat
      241 taaatcgggt tcgcgccaca gtttttttcc ggtatccgat actaatacaa tatgatcaac
      301 ataagggcgg tgcaaatcaa actgaggcga gtttgggtca ataaactcca ggtgtttttc
      361 gtcaataaaa ccggcatcaa tggccttttt catctcgata cgcagctcat caattgaacc
      421 aatgcatttt tcttcaccat catcgtttcg ccaaataggt aagggtatgc cccaaaaacg
      481 cgaacgcgat aaattccaat ccactaaatt ttcgagccaa tttccaaaac ggcctgtgcc
      541 ggtactttcg ggtttccagt taatggtttt attaagagca agcatttggt ctttatatgc
      601 agtggttcgt ataaaccagc tatctaaagg ataataaagt acgggtttat cggtacgcca
      661 acaatgcggg tagctgtgct cgtagcgggc tactttaaat gctttgtttt cggttttaag
      721 tttgatggca atgcgctcgt caacgctcag gtagttttcg cgaccttgtt gttttttagc
      781 cgcttctaat tcgttttcgg ttaagtattc ctgtttcaca tattcagatg caaaatcggt
      841 tacatgtttt acaaaacggc cttgcttatc tactaaaggc atgggtttgc ctgcatcatc
      901 ggttactaat atggcttcga tacctgcctg ctttgcaaca cgtgcatcat cggcaccaaa
      961 ggttggggca atgtgtacga taccggttcc gtcttcggta gaaacaaaag ctccggctat
     1021 aacctca
//
LOCUS       DAOMAA010001025         1026 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_207561_length_1026_cov_1.959835, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010001025 DAOMAA010000000
VERSION     DAOMAA010001025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_207561_length_1026_cov_1.959835"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="PLO59_11565"
     CDS             <1..398
                     /locus_tag="PLO59_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009392731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HQV01794.1"
                     /translation="ENKITRVGGEKELSVNVRVLAATNKDLKKEIENGNFREDLYHRL
                     SVIVIQVPSLNERKSDIPILANHFVKEICEESSMPIKLFTDKALAELQKNNWTGNIRE
                     LRNVVERLLILCENNITEKDVQLFANYGK"
     gene            complement(399..>1026)
                     /locus_tag="PLO59_11570"
     CDS             complement(399..>1026)
                     /locus_tag="PLO59_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HQV01795.1"
                     /translation="AINVAAVLPVVYFIVLAPVIGMMLSYIISVISLHLCKRANPSKV
                     DKLFRRLQLLSSAAYSLGHGGNDAQKVMGIITAAMLSQNMIVDIRHLPEWVPLACYSA
                     IAAGTMIGGWRIVKTMGQKITKLSPFEGFSAETAGALTLFGTQHFKIPVSTTHTITGS
                     IIGVGVTKRISAVRWGVTLQLIWAWVLTIPVSAGLAALLFYIFSLFLY"
BASE COUNT          370 a          202 c          187 g          267 t
ORIGIN      
        1 aagaaaataa aataaccaga gttggaggcg aaaaagaatt gagcgttaat gttagggtac
       61 tggcagcaac taataaagat ttaaaaaagg aaatagaaaa tggtaatttc cgtgaagatt
      121 tataccatcg cttaagcgta attgtaattc aggtgccatc gcttaatgag cgtaaaagcg
      181 acatacctat tttagccaat cattttgtaa aagaaatttg tgaagaaagt tccatgccaa
      241 taaaattgtt cactgataaa gcgcttgccg aattgcaaaa aaataattgg actggtaata
      301 ttcgcgaatt acgaaatgtg gttgaacgct tacttatact ttgcgaaaac aatataaccg
      361 aaaaagatgt acaacttttt gcaaactatg gcaaatagtt aatacaaaaa caaactgaat
      421 atataaaaaa gtaaagccgc caagccggcc gaaaccggta ttgtaagtac ccaagcccaa
      481 ataagttgta aggtaactcc ccaacgtact gccgatatgc gtttggtaac acccacacca
      541 ataatacttc cggtaatggt atgtgttgta cttaccggta ttttaaagtg ttgcgtgcca
      601 aataaagtta gcgcgcctgc tgtttcggca ctaaaccctt cgaatggtga aagcttggtt
      661 atcttttgcc ccatggtttt aacaatacgc caaccaccta tcatggtgcc ggcagctatt
      721 gccgagtaac atgccaatgg aacccactct ggtaaatgcc taatatcgac aatcatattt
      781 tgcgatagca tagcagctgt gataataccc ataacctttt gtgcatcatt tcctccatgc
      841 cccaaactat aagcggccga tgaaagtaat tgcaagcgcc tgaataattt atcaaccttg
      901 ctcggattag cccttttaca taaatgcaaa gaaataacac ttataatata cgaaagcatc
      961 ataccaatta caggcgctaa aacaataaaa taaacaacag gtaaaacagc agcaacatta
     1021 atggca
//
LOCUS       DAOMAA010001026         1024 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_208250_length_1024_cov_2.542828, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010001026 DAOMAA010000000
VERSION     DAOMAA010001026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_208250_length_1024_cov_2.542828"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="PLO59_11575"
     CDS             <1..557
                     /locus_tag="PLO59_11575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01796.1"
                     /translation="EYATFFAAQDAMNIKISSVLRLNSTFPYVSPVVSLPSVPPIEVM
                     DSGIRDNFGITTSARYMMVCKQWIEENTSGVIVLQIRDTYKQAAIQQSGAPTYMQKLA
                     APFRNLSGNFLLMQDYSNDALIAMVQSSIKVPVSTVNFELPDSEHKVSLSWHLTKRER
                     MFTLAQMYNAYNQNQLQQLKQLLR"
     gene            complement(549..>1024)
                     /locus_tag="PLO59_11580"
     CDS             complement(549..>1024)
                     /locus_tag="PLO59_11580"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HQV01797.1"
                     /translation="LYFKQNNPETKLLVSDIRQDVLQEAGNRFKKTGLKKIEIKMHDA
                     VMPLDQRFDGIIVDAPCSGSGTWKRAPEHYRHFDVLSLNSFKHRQIALIKNAWLHLKP
                     GGKLIYITCSMFAAENEDVINEANQFCNAHVSHTVINGNVYGADIMFRAVFTKSS"
BASE COUNT          315 a          200 c          183 g          326 t
ORIGIN      
        1 ttgagtatgc aacttttttt gcagcacagg atgcaatgaa tataaaaatc agcagtgtgt
       61 tgcgtttaaa ttctacattc ccttatgtat cgcctgtagt ttcattacca tctgtaccac
      121 ctatcgaggt aatggattcg ggtattcgcg ataattttgg tattacaaca tcggctcgtt
      181 atatgatggt atgtaagcaa tggatagaag aaaacacttc aggtgttatt gtattacaaa
      241 tacgtgatac ttataaacaa gcagccatac aacaaagtgg tgcacctaca tacatgcaaa
      301 aactggccgc accttttcga aacttatcag gcaacttttt attgatgcag gattatagta
      361 atgatgcatt aatagcaatg gttcagtcgt caattaaagt gccggtttca actgtaaatt
      421 tcgagttgcc tgatagtgag cataaagtat cgctaagctg gcatttaacc aagcgcgaac
      481 gcatgtttac attagctcaa atgtacaatg cttataacca aaatcagtta caacaactaa
      541 agcagctatt aagatgattt tgtaaaaacg gctctaaaca taatgtcggc accataaaca
      601 ttgccattta taaccgtatg gcttacatgg gcattacaaa attgatttgc ttcattaata
      661 acatcttcgt tttcggcagc aaacatgcta caagtaatgt aaatcagttt accacccggt
      721 tttaaatgaa gccatgcgtt tttaattaaa gctatttgac ggtgcttaaa actatttagg
      781 cttaaaacat caaagtgcct ataatgttcc ggtgcacgtt tccaggtgcc cgatccactg
      841 caaggtgcat ctacaataat gccgtcaaac ctttgatcca aaggcataac ggcatcgtgc
      901 attttaattt caattttttt tagccctgtt ttcttgaaac ggtttccggc ttcctgcagt
      961 acatcctgtc ttatgtcgct aaccaataat ttcgtttccg gattgttttg tttaaagtac
     1021 aaac
//
LOCUS       DAOMAA010001027         1023 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_208617_length_1023_cov_2.788223, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010001027 DAOMAA010000000
VERSION     DAOMAA010001027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_208617_length_1023_cov_2.788223"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1023
                     /locus_tag="PLO59_11585"
     CDS             <1..>1023
                     /locus_tag="PLO59_11585"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQV01798.1"
                     /translation="SHGAGAEVQKPLATVVIGGIASATLLTLFVLPLLYMLFSTKKQI
                     KSARSNLVTLFVLCFLFGNLLPQHVNAQNTLTLQQALDTALKNNLQLQAKQQYMQAAM
                     AQKKSCFELPKTEINFQYGQYNSIQKDNGFSIQQLIPFPSYYKAKSDLYKTHIAETQL
                     QQQVTINDVTTQVKSLYYQLLYLNQVHGELKYILLKYEGFSKIAALKFKAGQTDALEQ
                     TTATIKHGQMLLQLQQNEAEIAMAYTSLKSLLNYNDSFLVDTSIAFEPLIIADIIDTN
                     ALSKSPLIKLPYQQASIAAANKKIETVQTLPDFKIGYLNQTLIGYQNVNGSDIYYNSN
                     QRFTGFN"
BASE COUNT          377 a          203 c          160 g          283 t
ORIGIN      
        1 tctcatggtg ccggagctga agtgcagaaa ccacttgcta cggttgttat tggtggtatt
       61 gcctcagcta ctttacttac cttgtttgtg ctgccattac tttacatgct gttttcaact
      121 aaaaaacaaa taaaatctgc ccggtcaaat ttagttacac tgtttgtact atgctttctt
      181 tttggcaatc tgttaccgca acatgtaaat gcacaaaata cattaacatt acaacaagcg
      241 ctcgataccg cactaaaaaa caatttgcag ttacaggcaa aacagcagta tatgcaagct
      301 gcaatggcac aaaaaaagtc gtgtttcgaa ttacctaaaa ccgaaattaa ctttcaatac
      361 ggccaataca acagtattca aaaagataac gggtttagca ttcagcaatt aattccattc
      421 ccatcctact ataaggctaa atcagattta tataagacac acattgccga aacacaattg
      481 caacagcagg ttaccattaa tgatgtaacc acacaagtta aatcattgta ttatcagttg
      541 ctttacttaa atcaagtaca tggtgagttg aaatatatat tgttgaagta cgaaggattt
      601 tcaaagattg cagcgctaaa atttaaagcc ggacaaacag atgcattaga gcaaacaacg
      661 gcaactataa aacacggaca aatgttattg cagttgcaac aaaacgaagc cgaaattgca
      721 atggcataca catcgcttaa atcactttta aattacaacg attcattttt agttgataca
      781 tcaatcgcat tcgaaccgct gataattgct gatataattg atacaaatgc gctttcgaaa
      841 agtccactga taaaattacc atatcaacaa gcaagtattg cagcagcaaa caaaaaaata
      901 gaaaccgtac aaacattgcc tgattttaaa atagggtacc tcaatcaaac acttatcgga
      961 tatcaaaacg taaatggcag cgatatatac tacaacagca atcaacgttt taccggattt
     1021 aat
//
LOCUS       DAOMAA010001028         1023 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_208692_length_1023_cov_2.322314, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010001028 DAOMAA010000000
VERSION     DAOMAA010001028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_208692_length_1023_cov_2.322314"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..829
                     /gene="gldF"
                     /locus_tag="PLO59_11590"
     CDS             95..829
                     /gene="gldF"
                     /locus_tag="PLO59_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     permease subunit GldF"
                     /protein_id="HQV01799.1"
                     /translation="MFALIRKEINGFLNSLIGYVVILVFLITISLFLWVFPDSEFNIL
                     SNGYASLEPLFTITPWVYMFLIPAITMRLFAEEKKTGTLELLITRPLTELQIVFAKYI
                     AGVILVFISLLPTLVYYVTVYQLGLTIGNMDSAATWGSYLGLLLLGACFVAMGVFASA
                     LTDNQVIAFIISLFLCLFFYIGFDRISAMAFTGALSQLIYQTGIYAHYVSMSRGVIDT
                     RDLVYFLSLIALFIVLTRTIIESRKW"
BASE COUNT          290 a          166 c          191 g          376 t
ORIGIN      
        1 cgggtataaa tagccttttt acttcaattt aaaaattgat tatggcccta aagtgtaatt
       61 attattttcg catgcttttt taaagcccta tggcatgttt gcacttataa gaaaagagat
      121 aaacggtttt ttaaattcgc tgattggcta cgtagtaatt ctggtatttt taattaccat
      181 cagtttgttt ctatgggtgt ttcccgatag cgagtttaat attttaagca atggctatgc
      241 atcgctcgag cccttgttta ccattacccc ttgggtatat atgtttttaa ttccggctat
      301 taccatgcgc ttgtttgccg aagaaaaaaa aaccggtacg cttgaactgc tcattacacg
      361 accgcttacc gaacttcaaa ttgtatttgc caaatacata gccggagtaa tattagtttt
      421 cattagtttg ttaccaacct tggtatatta tgtaacggtt taccaattag gcttaaccat
      481 tggcaatatg gattcggcag ctacatgggg cagttacttg ggtttgttgc ttttaggtgc
      541 ttgttttgta gctatgggtg tatttgcaag tgcactaacc gataatcagg taattgcatt
      601 tataataagc ctatttctgt gtctgttttt ttatatcggt tttgacagga ttagcgccat
      661 ggcttttacg ggtgcattaa gtcaactcat ttaccaaaca ggtatttatg cacattacgt
      721 tagtatgagc agaggtgtaa ttgatacacg cgatttagtt tactttttaa gtttaattgc
      781 actatttatt gtattaaccc gaaccattat tgaaagccgt aagtggtaaa ttattttaat
      841 cagatagtat aaaggcgtgt tgaaacgaag tcataaaaat caaaatatta taaacctgct
      901 attaacctta gcggttattg ttgttattaa tgtattgagt tattacattt ttgcacgtat
      961 tgatttaaca gccgataaac gctatacctt gcataaaagc accgagcagt tgcttagtaa
     1021 att
//
LOCUS       DAOMAA010001029         1022 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_209042_length_1022_cov_2.641158, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010001029 DAOMAA010000000
VERSION     DAOMAA010001029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_209042_length_1022_cov_2.641158"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="PLO59_11595"
     CDS             <1..202
                     /locus_tag="PLO59_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009217957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01800.1"
                     /translation="SKRVVGVTLHSGRNRIVRRIFEHLGYMVIKLDRTYYAGLTKRDL
                     PRKRYRMLTESEINMLKMFKAH"
     gene            complement(282..752)
                     /locus_tag="PLO59_11600"
     CDS             complement(282..752)
                     /locus_tag="PLO59_11600"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HQV01801.1"
                     /translation="MQHTYQTNIKWQNTAPLKPFTYTSYSRNHSLHIDGKADLLCSSD
                     VVFRGDGSRYNPEDFLLASVSSCHMLWYLHLCADNGITVVDYNDTATGVLQTGANQPG
                     KFVAITLHPNVTITEAAHIQLAHELHHKANTHCFIANTLNLEIKHIVTINALTK"
     gene            complement(763..>1022)
                     /locus_tag="PLO59_11605"
     CDS             complement(763..>1022)
                     /locus_tag="PLO59_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01802.1"
                     /translation="EKYQLSPNWDSMGVYIPPKDFDLSGNVMRSVYLIKKYGIEQMMH
                     EKDNQLKDASPELIESILEEKMLLQQMYMLINKYLGIVITG"
BASE COUNT          304 a          182 c          200 g          336 t
ORIGIN      
        1 tagcaaacgt gtagtaggtg ttaccttaca tagtggccgc aaccgcatag tgcgtagaat
       61 ttttgaacat ttgggatata tggtaattaa gctcgaccgt acttattatg ccggactaac
      121 caaacgcgat ttgccccgca aacgttatcg catgcttacc gaaagcgaaa taaacatgct
      181 caaaatgttt aaagcacatt aagcacaaaa caaattttat cactaatgcc ataaacgtaa
      241 aatggttgat aatgaattgt tgcacgattg tgtatcgtta tctacttcgt taatgcatta
      301 atggttacta tatgctttat ttccaaattt aatgtattcg caataaagca atgtgtgttg
      361 gctttgtgat gcaattcatg tgctaactga atatgggcag cctcggtaat ggttacattc
      421 ggatgtaatg ttatggcaac aaattttccg ggttgattag cgcctgtttg taaaacaccg
      481 gttgctgtat cgttataatc aaccacagta atgccgttat cggcacagag gtgcaaatac
      541 caaagcatgt ggcatgaaga taccgatgcc aataaaaagt cttcggggtt atagcggctg
      601 ccatcacccc tgaatacaac atcggatgag cataacaaat cggctttgcc atcaatatgc
      661 aaagaatggt tgcgtgagta tgatgtatat gtaaaaggtt taaggggtgc tgtattttgc
      721 cacttaatgt ttgtttggta agtatgttgc ataagctttg tgttaaccgg taattacaat
      781 gcctaaatac ttatttataa gcatgtacat ttgttgtaat agcatttttt cttccaaaat
      841 gctttcaatt aattctggtg aagcgtcttt taactgatta tctttttcat gcatcatttg
      901 ttcaatgcca tattttttaa tcaaatacac acttcgcatt acattgccac ttaggtcaaa
      961 atctttgggt ggtatgtaaa cgcccatgct atcccaattg ggactaagct gatatttttc
     1021 ag
//
LOCUS       DAOMAA010001030         1022 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_209067_length_1022_cov_2.421923, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010001030 DAOMAA010000000
VERSION     DAOMAA010001030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_209067_length_1022_cov_2.421923"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="PLO59_11610"
     CDS             <1..198
                     /locus_tag="PLO59_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQV01803.1"
                     /translation="MYACYVLFAKLTGMVHVEGWSALMVVFLLVSSFQSIALGIIGEY
                     LWRNLEHSRNRPAYVVDEVYK"
     gene            200..>1022
                     /locus_tag="PLO59_11615"
     CDS             200..>1022
                     /locus_tag="PLO59_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01804.1"
                     /translation="MDALHNQIGWVFLILIISIFVLLVIGHVVKALLGLASADKTGYY
                     ALLTGTVFFVLFSATILSCSKTIMLGFVLIFVFLNNKPYKIKWPSINNVLHVVIIASA
                     FFTIQYLTIFNGNELPLLPEGDALYYARLASKMAETGIENTNLYGNEGREPYHYFDLW
                     ITATLIKFTKFNPYLILKLIVYPILLTILYAGYFLWACSKNLSKLNSALLALLLCFFS
                     GFYLLAFDKINYVNDSVLFFYSAITYPKLLVIYIFIQAAFISYISKNNFNAITWLA"
BASE COUNT          294 a          155 c          187 g          386 t
ORIGIN      
        1 atgtatgctt gctatgtgct gtttgcaaaa cttacgggca tggtacatgt tgaaggatgg
       61 agtgcattaa tggtagtttt tttattggta tcctcctttc aatcaattgc actgggcatt
      121 attggcgaat atttatggcg caacctggag cacagtcgaa acagacctgc ttacgtagtt
      181 gatgaggtat ataaataaga tggatgcatt acataatcaa attggctggg tatttctgat
      241 tttgattata agcatttttg ttttgcttgt aataggtcat gtggtaaaag ctttattagg
      301 tttagcctct gctgataaaa ccggctatta tgcattacta acaggcactg ttttttttgt
      361 ccttttttca gcaactattt taagttgttc taaaaccata atgttaggct tcgtattgat
      421 ttttgttttt cttaataata aaccttataa aataaaatgg ccctcaataa ataatgtttt
      481 gcatgttgta attattgcat ctgctttttt tactattcaa tatctaacaa tttttaacgg
      541 caatgagttg ccgctgttgc ccgaaggtga tgcattgtat tatgcacgat tggcaagcaa
      601 aatggccgaa accggaattg aaaacaccaa cctgtatggc aatgaaggca gggagcccta
      661 tcattatttt gatttgtgga taacggctac gcttataaaa tttacaaagt tcaatcctta
      721 cctgatatta aagctaattg tatatcctat tttgcttaca attttatatg caggttattt
      781 tttatgggca tgcagtaaaa atttaagtaa attaaactcg gcattgttgg ctttgctgct
      841 gtgtttcttt tcaggatttt atctgctggc tttcgataaa attaattatg taaacgatag
      901 cgtattgttt ttttatagcg caataacgta ccccaaatta ttggttatat atatttttat
      961 tcaagctgct tttataagtt acatcagtaa aaataatttc aatgccataa cctggttagc
     1021 at
//
LOCUS       DAOMAA010001031         1021 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_209478_length_1021_cov_2.363354, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010001031 DAOMAA010000000
VERSION     DAOMAA010001031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_209478_length_1021_cov_2.363354"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1021)
                     /locus_tag="PLO59_11620"
     CDS             complement(<1..>1021)
                     /locus_tag="PLO59_11620"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HQV01805.1"
                     /translation="VSDIPTIAPVLSKSESNRLLIIKALLHAELNNLKISDANDTQLL
                     LKLLQQKQHAYNAQDAGTVMRFLTAYLAAKPGMHVLTGTERMMQRPIGALVKALNQLG
                     AKIDYANAIGFPPLKIEGRSLTGNYVNMDAGISSQFISALLLVAPYFLKPLTLNLQGH
                     TVSEPYIETTIAMMQHFGVNVVKQEQTYRVPVMHYTDSDFAVEADWSAAAYYYALVAL
                     GVVSQLTLKGYKANSLQADSALVHVFETLGVNTIFKPDGITLFNKKSSLPQQIDLNCI
                     TFPDAVPAIAVACAGLGIAANLTGLQTLVIKETNRIKALQSNLQLCGFNVASTSNSLS
                     INGKL"
BASE COUNT          282 a          216 c          201 g          322 t
ORIGIN      
        1 aacaacttgc cattaataga taaactattt gatgtacttg caacgttaaa accacataat
       61 tgcaaattac tttgcaaagc tttaatgcgg ttggtttctt taattaccaa ggtttgcaaa
      121 cctgttaggt tggctgcaat gcctaagcct gcacaggcta ctgcaatggc cggcactgca
      181 tccggaaagg ttatgcaatt taaatctatt tgctgcggca aactgctttt tttattaaat
      241 agtgtaatgc catccggttt aaaaatggtg ttaacgccca aagtttcaaa tacatgtacc
      301 aatgcactat cggcttgcaa actatttgcc ttatagcctt ttaatgttag ctgactaaca
      361 acacccaggg cgaccagtgc ataataataa gcggctgcac tccaatctgc ttccactgca
      421 aaatcgctat ctgtataatg cataacgggt accctatacg tttgttcttg tttaaccacg
      481 tttacaccaa aatgctgcat catggcaatg gttgtttcga tgtatggttc tgataccgta
      541 tggccttgca ggttaagtgt aagtggtttc aaaaagtaag gtgctacaag caacaatgca
      601 cttataaact gactgctgat accggcatcc atatttacat agttgccggt taaacttcgg
      661 ccttctattt tcaaaggcgg aaaaccaatt gcatttgcgt aatcaatttt tgcacccaat
      721 tggtttaagg ctttaaccag tgctccaatt ggccgttgca tcatacgttc tgtgccggtt
      781 aatacatgca tacccggttt agcagccagg taagcggtta aaaaacgcat tacggtgccg
      841 gcatcctggg cattatatgc atgctgtttt tgttgcaata gtttcagcaa cagttgcgta
      901 tcgtttgcat ccgaaatttt taagttgttt aactcagcat gcaacaaagc ttttataatt
      961 aacagcctgt tgctttcgct cttcgaaagt accggagcta tggttggtat atcgcttacg
     1021 t
//
LOCUS       DAOMAA010001032         1021 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_209658_length_1021_cov_1.516563, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010001032 DAOMAA010000000
VERSION     DAOMAA010001032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_209658_length_1021_cov_1.516563"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1021)
                     /locus_tag="PLO59_11625"
     CDS             complement(<1..>1021)
                     /locus_tag="PLO59_11625"
                     /inference="COORDINATES: protein motif:HMM:NF025009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HQV01806.1"
                     /translation="NDFNKKYESNLKSAEDQMRGQPMGDDQRNYLRTQTWTEMVNDII
                     FKDVYDKLGINVTPDEMGELATGENASPYIKNDQQFKNPQTGQFDPAQVRLYVSQLDQ
                     PREGVEPGLIRDQWLRFEKLLKQNQFQTKYNNLITKGLYVPTWMAEMTYNDQNSFADF
                     KYVVLPFSEVNDADVKVEDSDIKNYISSHSAKFKVDEETRKFQYVAFDIAPSAQDSAK
                     TIELLEEKRAEFAKGEKASDDSLFVKIYSETPFDNVYYDKDKLTSSVKDSFFSLPVKS
                     LVGPYFDGNVAKLAKISDRKMISDSVRVREIVISFEGIIIHDQAKTTERFKLMDSLVR
                     VIDTLK"
BASE COUNT          262 a          215 c          211 g          333 t
ORIGIN      
        1 cttaagggta tcaattacgc gcacaaggct atccatcaat ttaaacctct cggtggtctt
       61 agcctgatca tggatgataa tgccttcaaa cgaaataact atttcgcgca ctcgaaccga
      121 atctgaaatc attttgcggt cgctgatttt agccagctta gccacgttgc catcaaagta
      181 agggcctact aaagatttaa ccggtaagct gaaaaatgaa tctttaaccg atgatgtaag
      241 tttatcttta tcgtaataaa cattatcgaa aggagtttcg gagtaaattt taacaaacaa
      301 cgaatcgtct gatgcttttt cgcctttggc aaactctgca cgtttttctt ctaaaagctc
      361 gatagtttta gccgagtctt gcgccgaagg tgcaatatcg aaagctacat actggaactt
      421 gcgggtttct tcatctactt taaacttagc cgagtggctg ctgatgtagt ttttaatatc
      481 gctgtcttca acttttacat cagcatcgtt tacttcagaa aaaggcaata ccacatattt
      541 aaagtcggca aagctgtttt ggtcgttgta agtcatttcg gccatccagg taggtacata
      601 caagcctttt gtaataaggt tattgtattt ggtttgaaac tggttttgtt ttaaaagctt
      661 ttcaaaacgt aaccactggt ctctgattaa cccgggctct acaccttcgc gcggctggtc
      721 taactggctt acatataaac gaacctgtgc cggatcgaac tgaccggttt gtgggttttt
      781 aaactgctgg tcgtttttaa tataaggcga tgcgttttcg ccggttgcca attcgcccat
      841 ttcatcaggc gttacgttaa tgcccagctt atcgtaaaca tctttaaaaa taatgtcgtt
      901 aaccatttcc gtccaggttt gggtgcgcag gtaattgcgc tggtcgtcgc ccataggttg
      961 gccacgcatc tggtcttcgg cacttttaag gttgctttcg tactttttat taaagtcgtt
     1021 g
//
LOCUS       DAOMAA010001033         1020 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_209794_length_1020_cov_2.923316, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010001033 DAOMAA010000000
VERSION     DAOMAA010001033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_209794_length_1020_cov_2.923316"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1020)
                     /locus_tag="PLO59_11630"
     CDS             complement(<1..>1020)
                     /locus_tag="PLO59_11630"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HQV01807.1"
                     /translation="YANQVKSQLFINEVSCSNLSQFADNHSDYGDWFEIYNAGASAVN
                     LQGYYLSDDSLNNLKWQFPSSINIAANGFMRIWASGRNEIVGNSVHTNFKLTQTKSNK
                     EEVTLSDPSGIIISQVKLTEKTQLGHSYGRTTNGAATWSVFVTPTLASSNNTATSYSR
                     YAMRPDFDIAPGFYNSTQTVSITTTEPVATIRYTTDGTLPTATSTIYTGPVTIASTKV
                     LKAIVFTTTPGVLPSFIQYGTYFINVSHTVPVVSITGTNLTQLANGNGNLEPFGSFEF
                     FDTLKLSKARTYGEFNQHGQDSWANSHRSLDFVSRDEMGYNSAIKEQLFSMSNRDEFQ
                     RIILR"
BASE COUNT          264 a          187 c          240 g          329 t
ORIGIN      
        1 cacgtaaaat aatgcgctga aattcatcgc ggttcgacat gctgaacaat tgctctttga
       61 ttgccgagtt ataacccatt tcatcgcgcg atacaaaatc taaactgcgg tggctgttag
      121 cccatgagtc ttgtccatgt tggttaaact caccataagt gcgtgcttta cttagcttta
      181 atgtgtcaaa aaattcgaac gatccgaaag gctccaggtt gccattacca tttgcaagtt
      241 gcgtaaggtt tgtacctgta atggaaacaa cgggtacggt atgcgataca ttaataaaat
      301 aggtgccata ttgtataaag cttggcaaaa cgcccggtgt tgtggtaaac acaattgctt
      361 ttaaaacttt agttgatgcg atggtaacgg gccctgtata aattgttgat gttgctgttg
      421 gtaatgttcc gtctgtagtg taacgtattg ttgcaaccgg ttcggtggta gttatgctta
      481 cggtttgtgt gctgttataa aagccgggcg caatatcaaa gtcggggcgc atggcgtagc
      541 gactatagga agtggctgtg ttattagaac ttgctaatgt gggtgtaaca aatacactcc
      601 atgtggctgc cccattggtt gtgcgaccat atgagtgacc caattgggtt ttttcggtaa
      661 gttttacctg actgatgatt atacccgatg gatctgatag tgtaacttct tctttattgc
      721 ttttggtttg tgtaagttta aaattggtgt gtacgctgtt gccaactatt tcgttgcggc
      781 ccgaagccca aatgcgcata aatccgttgg ctgcaatgtt tatggatgac ggaaattgcc
      841 atttcaaatt gtttagcgag tcgtcactta aataatatcc ttgtaagttt actgctgaag
      901 cacctgcatt gtatatttca aaccaatcgc cataatcgct gtggttatcg gcaaattgcg
      961 aaaggttaga acaggatact tcgtttatga aaagctggct ttttacttga ttggcataac
//
LOCUS       DAOMAA010001034         1020 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_209920_length_1020_cov_2.149223, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010001034 DAOMAA010000000
VERSION     DAOMAA010001034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_209920_length_1020_cov_2.149223"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            212..754
                     /locus_tag="PLO59_11635"
     CDS             212..754
                     /locus_tag="PLO59_11635"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HQV01808.1"
                     /translation="MKANQFFKSPIGNLMVTASQQGVTGIGYVDDTNYDNSGLSLKSD
                     VCDAIDAGHTDFNSSKHIQLACMELQQYFDGHLKQFTFPIDLSGTNFQMHVWQQLQQI
                     EFGKTISYRKLALLLGDVKCIRAAASANGKNPLAIAIPCHRVIGSNGNLIGYAGGLWR
                     KKWLLQHEGVLPNFELNFNG"
     gene            767..>1020
                     /locus_tag="PLO59_11640"
     CDS             767..>1020
                     /locus_tag="PLO59_11640"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HQV01809.1"
                     /translation="MNNEVKHAFMAAFAFAIWCLLEFATGIHNNHMNLAQYTEMLGGF
                     IPWVFIVLAIYKRKHQLQNGQLTWGQAIRCGMPVALIASL"
BASE COUNT          320 a          164 c          204 g          332 t
ORIGIN      
        1 ccttcggttt ctaaaatgat ttttgtaaaa tgctccacat ttttttgtgt acgaacaatg
       61 gggccaacac catcaatttt tttattgcag ccccaacaaa atagtatcag aatcaaaagc
      121 gtaaaaacac gttgcatcat atctgtttgt tttaatttat ttggtaataa taaggtgtaa
      181 aatacaaatt acattgttta atttgttttg aatgaaagcc aatcagtttt ttaaatctcc
      241 aatcgggaat ctaatggtaa cagcaagcca acaaggtgtt acaggtattg gctatgttga
      301 tgatacgaat tatgataatt ccggtttaag tttaaaaagc gatgtgtgtg atgcgataga
      361 tgctggccat acagatttca attcgagcaa acacatacaa ttagcctgca tggaattgca
      421 gcaatatttt gatgggcatt tgaaacaatt tacttttccg attgatttat ccggcactaa
      481 ctttcagatg cacgtttggc agcaattgca acaaatagaa tttggcaaaa ccattagtta
      541 tagaaaattg gcgctattac ttggcgatgt taaatgtata agggctgcag caagcgctaa
      601 cggtaaaaat ccattagcca ttgcaatacc ttgtcatagg gtaattggca gtaatggaaa
      661 tttaattgga tatgcaggtg gcttgtggcg aaaaaaatgg ttattgcagc atgaaggtgt
      721 attaccaaat tttgaattaa attttaacgg ataaatattt atagcgatga ataacgaagt
      781 taaacatgct tttatggctg catttgcttt tgccatttgg tgcctgctcg aatttgctac
      841 gggcatacat aataaccata tgaatttggc acaatacact gaaatgcttg gcggttttat
      901 tccctgggtt tttattgtac tggctattta taaacgaaaa catcaattac aaaacggaca
      961 gcttacctgg gggcaggcca tacgttgcgg tatgcctgtg gcattaattg cttcgctgtt
//
LOCUS       DAOMAA010001035         1019 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_210204_length_1019_cov_2.961618, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010001035 DAOMAA010000000
VERSION     DAOMAA010001035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_210204_length_1019_cov_2.961618"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1019)
                     /gene="sprA"
                     /locus_tag="PLO59_11645"
     CDS             complement(<1..>1019)
                     /gene="sprA"
                     /locus_tag="PLO59_11645"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HQV01810.1"
                     /translation="YLNAASPISVTLESNSLFSIQSKRLMGLHVDYLHSKDFTLGGTI
                     LNLTERPLTQKINIGDEPISNTIVGVDGNYRTESRFITKLIDRLPFLETKEVSNVAVS
                     GEYAHFFPGHPKVIGRTGTSYLDDFEGTQSNIDLTTAGSWSLASPPQNQPTLFPETTS
                     PENRVFGVNRAKLAWYQIDPTVFYSNSSLRPASIPDEQLQNNQVRQVFDNELFPQKNY
                     PNGIAPPLRVLNLAYYPTERGPYNYDVSGVPGISAGINPDGKLLAPASRWGGMMRNLQ
                     TNDFEIANVEFITFWLMDPFNEDNPNAANNKGKLYFNLGQISEDILHDGFRLYENGLP
                     SNTLS"
BASE COUNT          280 a          197 c          227 g          315 t
ORIGIN      
        1 agctaagtgt atttgatggc aatccgtttt cataaagcct gaaaccgtca tgcaatatgt
       61 cttcgctaat ctgccccagg ttaaagtata atttaccttt gttattggct gcattggggt
      121 tgtcttcgtt aaaaggatcc atcagccaaa aggtaataaa ctcaacgttt gcaatttcaa
      181 aatcgttggt ttgcaggttg cgcatcatac caccccagcg cgaagccggt gccaataatt
      241 taccatcggg attaatacct gccgaaatac cgggcacacc gcttacatcg taattataag
      301 ggccacgttc cgtggggtag taagctaggt ttaatacacg taacgggggt gctataccat
      361 ttgggtaatt tttttgtgga aacaattcgt tatcaaaaac ctgccttacc tgattgtttt
      421 gtaattgctc atcgggtatg ctggcgggtc gtaaagatga gttgctgtaa aatacggtag
      481 ggtctatctg ataccaggca agtttggcac ggttaacacc aaatacacgg ttttcgggtg
      541 aagtggtttc gggaaatagt gtaggttggt tttgtggcgg acttgctaat gaccatgagc
      601 ctgccgtagt taaatcaatg ttggattgtg tgccttcaaa atcatctaaa taagatgtgc
      661 ctgtacggcc aataacttta ggatggcccg ggaaaaagtg tgcatactcg ccactaacgg
      721 caacattact tacttctttg gtttctaaaa acggtaacct gtcaattaat ttggtaataa
      781 agcgcgattc ggtgcggtag ttaccatcaa cacccacaat ggtgttactt ataggctcat
      841 cgccaatgtt tattttctgc gttaatggcc tttcggttag gtttaaaata gttccgccta
      901 aagtaaaatc tttactatgt aaatagtcca catgcaaacc cattaatcgt tttgattgta
      961 tgctgaataa tgaattactt tctaacgtaa ctgaaatagg gctggctgca ttaaggtat
//
LOCUS       DAOMAA010001036         1019 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_210354_length_1019_cov_2.067427, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010001036 DAOMAA010000000
VERSION     DAOMAA010001036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_210354_length_1019_cov_2.067427"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="PLO59_11650"
     CDS             <1..639
                     /locus_tag="PLO59_11650"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HQV01811.1"
                     /translation="VELCGGTHVPATGHIGLFKIVSESSVAAGVRRIEAITANEAVNY
                     YKKQEQTLQQINELLKFPKDTLKGMHALLDENAKLKQEVTANLMHKAADLKTELSLKG
                     FDIGNIHVIAEIVDLPNADAIKQMSFEMRNTIPNLFLLLGTVIDGKPHLSLLINDEVV
                     TTKNLNATAIIRVLAKEKKGGGGGQPFYATAGGKNVGGLQVAINKAKSLIGN"
     gene            715..>1019
                     /locus_tag="PLO59_11655"
     CDS             715..>1019
                     /locus_tag="PLO59_11655"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HQV01812.1"
                     /translation="MKNFKIPSVLKNKYVIAVLVFLVWLTFFDQNSFLVQYSYQQDLN
                     KLEKEHEYYKTQIDYNKAQLTQLETDTALLEKYAREKYYMKKKNEDIFVLVADSDST"
BASE COUNT          371 a          160 c          195 g          293 t
ORIGIN      
        1 gttgaattgt gtggaggcac ccatgtaccg gctactggcc atattggttt gtttaaaata
       61 gtatcagaaa gcagtgttgc tgctggcgta cgcagaattg aagccataac ggctaatgaa
      121 gcagttaact actataaaaa acaagagcaa acactgcaac aaataaatga actattaaaa
      181 tttccgaaag atacccttaa aggtatgcat gcattattag atgaaaatgc aaaactaaag
      241 caagaagtaa cggctaacct tatgcacaaa gctgcggatt taaaaactga attgtcgtta
      301 aagggttttg acattggaaa catacatgta attgccgaaa tagttgattt gcctaatgcc
      361 gatgccatta aacaaatgag ttttgaaatg cgtaacacca tacccaattt atttttgcta
      421 ctcggcactg tgattgatgg taaaccacat ttaagtttat tgattaacga tgaggtagta
      481 acaactaaaa acctcaatgc cactgccatt ataagagtat tagccaaaga aaaaaaaggc
      541 gggggtggtg gtcagccatt ttatgctact gccggtggta aaaatgtggg tggtttgcaa
      601 gtggcaataa acaaagcaaa atcattaata ggcaattaat gcgttaacag tatcgtgttg
      661 gtaaaacctg ctattgattg tacaagcagc ttaacatttg tttttatttg agatatgaaa
      721 aactttaaaa ttccatcggt gctaaaaaat aagtatgtta tagctgtgct ggttttttta
      781 gtctggctta ctttttttga tcaaaacagt tttttggttc aatatagcta tcagcaagat
      841 ttgaataagc ttgaaaagga acacgaatat tataaaacac aaatagatta taataaagca
      901 caattaacac aattagaaac cgatactgcc ttactcgaaa aatatgcccg cgaaaagtac
      961 tatatgaaaa aaaagaatga agatattttt gtacttgttg ctgacagcga ctcaacaca
//
LOCUS       DAOMAA010001037         1018 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_210593_length_1018_cov_3.442368, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010001037 DAOMAA010000000
VERSION     DAOMAA010001037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_210593_length_1018_cov_3.442368"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="PLO59_11660"
     CDS             complement(<1..215)
                     /locus_tag="PLO59_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01813.1"
                     /translation="MYPALIWALLIGGLCAIPGQDLPTADWLSLLKADKWIHVALFAI
                     LVFLTLKGINQPDNELFFQKYAVVGTLT"
     gene            complement(268..648)
                     /gene="gcvH"
                     /locus_tag="PLO59_11665"
     CDS             complement(268..648)
                     /gene="gcvH"
                     /locus_tag="PLO59_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HQV01814.1"
                     /translation="MNFPAELKYTKDHEWVRIEGDVATIGITDFAQSELGDIVYVDIS
                     TQGETLSRETVFGSVEAVKTVSDLFMPLSGEVLEVNPDLDATPEAVNSDPYGKGWMVK
                     VKLSDMAEIDALMTAADYTALVGA"
     gene            797..>1018
                     /locus_tag="PLO59_11670"
     CDS             797..>1018
                     /locus_tag="PLO59_11670"
                     /inference="COORDINATES: protein motif:HMM:NF037744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01815.1"
                     /translation="MNVDKHISALLYHHDCVIVTDFGGFVANYKPAFVNPSSHTISPP
                     AKRIAFNGSLTQNDGLLAQYIAHQMQMSYS"
BASE COUNT          308 a          221 c          181 g          308 t
ORIGIN      
        1 gttaaagtac ctactactgc atatttctga aaaaacaatt cgttatcggg ctgattaatg
       61 cctttaagcg tcagaaaaac caatattgca aacaatgcca catgtatcca cttatcagcc
      121 ttaagcaacg acagccaatc agctgtgggc aaatcctggc cgggtatggc gcaaaggcca
      181 ccaattaata aagcccaaat cagggcagga tacatgtgct taaacaacat aaatatggta
      241 tggcactttt atattgtagt gatatgttta tgcgcctact aaagcagtat agtcggctgc
      301 cgtcattaat gcgtctattt cagccatatc gctcaacttt actttaacca tccaaccttt
      361 gccgtatgga tcgctgttta ctgcttcggg tgtagcgtct aaatcggggt taacttcaag
      421 cacctcacca cttaaaggca taaataaatc cgatacggtt ttaacggcct ccaccgaacc
      481 aaaaaccgtt tcacggctta aagtttcgcc ttgtgtactg atgtcaacat aaacaatgtc
      541 gcccagttcg ctttgtgcaa aatcggtaat tccaatggtg gctacatcgc cttcaatacg
      601 tacccattca tgatctttgg tgtactttaa ttcagcagga aaattcatag ttaaatggtt
      661 taaatcaaaa tgtttaagta aatgataatg cacaaaaata attttttgct attgcaaccg
      721 aagcactaca cataattttt atttagagat tgattattgc tttttattta ctttgtgctt
      781 ttcaatggtt gcagaaatga acgtagataa acatataagc gcacttttat accaccacga
      841 ttgtgtaatt gttactgact ttggtggctt tgttgctaac tacaaaccgg cttttgtaaa
      901 tccgtcatca cataccatat cgccccctgc aaaacgcatt gcatttaatg gcagcttaac
      961 tcagaatgat gggctgttgg cgcaatacat tgcccatcaa atgcaaatga gctactcc
//
LOCUS       DAOMAA010001038         1018 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_210633_length_1018_cov_2.775701, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010001038 DAOMAA010000000
VERSION     DAOMAA010001038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_210633_length_1018_cov_2.775701"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..>1018)
                     /locus_tag="PLO59_11675"
     CDS             complement(107..>1018)
                     /locus_tag="PLO59_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007651031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HQV01816.1"
                     /translation="EQHRFGVQQRYKLWSKSKTVLPHVLVMTATPIPRTLAMTLYGDL
                     DVSIIDELPPGRQPIVTHLRFENNRLRVFGFLKEEIKKGRQVYIVYPLIDESEKMDYM
                     NLMQGYEAVVQYFQQPEYKVSIVHGKMKPADKDFEMQLFVSGKTQIMVATTVIEVGVN
                     VPNASIMVIESAQRFGLSQLHQLRGRVGRGAYQSYCILMADYKLSVDARQRLQTMCNT
                     NDGFVISEVDLKLRGMGDIQGTQQSGNMSLRLADLPKDKAILEQARQAAINLLETDAT
                     LTQPENKVVRNTLNNLAAQKGNWGKIS"
BASE COUNT          271 a          235 c          186 g          326 t
ORIGIN      
        1 tggcatagcc gctttggtgg tatcgcgcag caatgcaaaa aagtaactgt tactataaat
       61 gcaccttact ttgttgaagc ttttttcagc aacagccaac ttttaatcag gatattttac
      121 cccaattgcc tttttgtgca gctaggttat tcaatgtatt tcttactact ttattttcgg
      181 gctgggtaag cgtggcatcc gtttcgagca ggtttattgc tgcctgcctt gcctgttcta
      241 atatggcttt atctttaggc aaatcggcta aacgcaaact catgttacca ctttgttgtg
      301 tgccttgtat atcgcccata ccgcgcaatt ttaagtcaac ttcgcttata acaaaaccat
      361 cgttggtatt gcacatagtt tgcaaacgtt gacgggcatc aacactcaac ttataatcgg
      421 ccattaaaat gcaatacgat tgatacgccc cgcgccctac tctgccccgt agctgatgca
      481 attgcgataa accaaagcgc tgcgcacttt caataaccat tatggatgca ttaggaacat
      541 taacacctac ttcgataacg gttgttgcaa ccataatttg cgttttgccg cttacaaaaa
      601 gttgcatttc aaaatcttta tcagccggct tcattttacc atgtacaatt gaaactttgt
      661 attcgggttg ctgaaaatat tgcaccacag cttcgtagcc ttgcataaga ttcatgtaat
      721 ccattttctc gctttcatca atcaatggat atactatata cacttggcgg ccctttttaa
      781 tttcttcttt taaaaatccg aaaacgcgca aacggttatt ctcaaacctt aaatgcgtaa
      841 ctattggctg acgcccgggt ggcaattcat caataatact tacatctaaa tcgccataca
      901 gtgtcatggc taaggtgcgt ggtatgggtg ttgctgtcat aaccaaaacg tgcggcaaca
      961 ccgttttgct tttgctccaa agtttatagc gctgttgcac accaaaacga tgttgctc
//
LOCUS       DAOMAA010001039         1017 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_210962_length_1017_cov_3.994802, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010001039 DAOMAA010000000
VERSION     DAOMAA010001039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_210962_length_1017_cov_3.994802"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="PLO59_11680"
     CDS             complement(<1..921)
                     /locus_tag="PLO59_11680"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQV01817.1"
                     /translation="MHVLFITRWYPHAANLQLGVFIQKHAWAVSRYAQVSVLHVLQQN
                     QTDTYIVNVTDGMVKEITVSFKPSKWRIINGYRNLKSHIIGWNYAAKSGRPNLLHSHV
                     LYGTCMATWLLHLTKRLPYIYSEHWHGFTDGQFSELPVLKQWLFKITARYACAVTAVS
                     PFLSNAMVTCGLRRRVEVIGNVVCKSDNKAPVSSDKINILSVADLIPLKNIGGIIEAI
                     AKAQTNHPQIVYQIVGDGPQMKMLQQKALTLLPENSFIFYGMLPNDKVLELINASSFI
                     VINSTVETFSVVAAEALLCGKPLVTTRCGGN"
BASE COUNT          328 a          204 c          159 g          326 t
ORIGIN      
        1 gttaccaccg catcgagttg ttaccaaggg tttaccacaa agtagggctt cggcagctac
       61 cacactaaag gtttcaaccg tactgttaat cactataaaa ctgcttgcat taattaattc
      121 taatacttta tcatttggca acatgccata aaaaataaaa gaattttcag gtaataatgt
      181 aagcgctttt tgttgcaaca ttttcatttg aggaccatcg ccaactattt gataaacaat
      241 ttgaggatgg tttgtttgcg cttttgcaat ggcttcaata atgccaccaa tgttcttaag
      301 tggtattaaa tcagctacac ttaaaatatt aattttatca gaagatacag gcgctttgtt
      361 atccgactta cacaccacat tgccaataac ttcaacccgc cttcttaatc cacatgtaac
      421 catagcatta cttaaaaagg gcgaaacagc tgtaactgca catgcatacc ttgccgtaat
      481 tttaaatagc cattgtttta aaacaggcag ttctgaaaat tgaccatcgg taaacccatg
      541 ccagtgttca ctataaatat atggcaacct tttggtcaaa tgaagcagcc aggtagccat
      601 acaggttcca tataaaacat ggctatgcaa caagttaggc ctgcctgatt ttgctgcata
      661 attccatcca attatatgtg attttaaatt tcggtaacca tttataattc tccatttcga
      721 tggtttaaag ctgacggtaa tttcctttac cataccatct gttacattta ctatataggt
      781 atcagtttga ttttgttgta atacatgtaa tacacttact tgtgcgtacc tgcttactgc
      841 ccatgcatgt ttttgtataa aaacacccaa ttgtaaattt gcagcatgcg ggtaccacct
      901 ggttataaat aaaacgtgca tcagtgaaaa attgcttttt aaaattaatc aaccatgtaa
      961 taaataactc aagcttaaaa acctcaaaag ctcactaatt tctaacgggt ggtaaag
//
LOCUS       DAOMAA010001040         1017 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_210979_length_1017_cov_3.361746, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010001040 DAOMAA010000000
VERSION     DAOMAA010001040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_210979_length_1017_cov_3.361746"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..153)
                     /locus_tag="PLO59_11685"
     CDS             complement(<1..153)
                     /locus_tag="PLO59_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018676623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HQV01818.1"
                     /translation="MSNIVAVIGRPNVGKSTLFNRLTESRKAIVDPTAGVTRDRHYGK
                     AEWNGKE"
     gene            340..>1017
                     /locus_tag="PLO59_11690"
     CDS             340..>1017
                     /locus_tag="PLO59_11690"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQV01819.1"
                     /translation="MKKISLFFLFALTLFCFQPGLAATKKEKSASPKGDKAFTEARYY
                     DAVNSYKKAFTKSKKAPTQAQAAYQAGECYRMMSNWKSAEEWYGKAVQAKSKNPLATL
                     RYADALRANGKYDEATAQYNAYKTLAPSDPAGEMGVQSSATAQGFKDKPTRHRIENAT
                     ALNTVYHDFGTAISKSDKNTVYFTSSRVEASGKDNDPWYGEKRFDIFKSTRDNNGRWS
                     TPIALGDI"
BASE COUNT          326 a          207 c          199 g          285 t
ORIGIN      
        1 ttctttgcca ttccattcgg cttttccata atgcctgtca cgggttacac cggccgttgg
       61 gtcaacaatg gctttgcgcg attcggttaa acgattaaaa agagtggatt tacccacatt
      121 gggccttcca ataacagcca caatatttga catgattatt aattttaaaa agaggccaaa
      181 ggtattttat tttaccattt aatttgggta taaatgtagt aaagtaagct attttaaagg
      241 ctttgaagcg attcaagttt ttttgatgcc tttgttagta tgaaatattt tttttccttt
      301 gcccaccatc ataaaacttc agtgtaattt aataactcta tgaaaaaaat atcccttttc
      361 tttctgtttg cacttaccct attttgtttt cagccaggtt tggcagctac taagaaagaa
      421 aaatcagcat cacccaaggg cgataaagcc tttaccgaag cccggtatta tgatgcggta
      481 aacagctaca aaaaagcatt tacaaaatca aaaaaggcac ctacgcaagc tcaggctgct
      541 tatcaggcag gtgagtgcta ccgtatgatg agcaactgga aaagtgccga agagtggtac
      601 ggcaaagcgg tgcaggcaaa atctaaaaat ccgttggcaa cattacgtta tgcagatgcc
      661 ttacgtgcca atggcaaata tgatgaggcc actgcgcaat acaatgcata taaaacctta
      721 gcaccttcag acccagccgg tgaaatgggt gtacaatcat cagcaacagc gcaaggtttt
      781 aaagataaac caacccgcca ccgtatcgaa aatgcgactg cattaaatac agtttatcac
      841 gactttggta cagcaatatc gaagtctgat aaaaacaccg tttactttac atcatcacgc
      901 gttgaagcat cagggaaaga taatgaccca tggtatggcg aaaaacgatt tgacattttt
      961 aaatcaacac gtgataacaa tggccgttgg agtacgccta ttgcattagg tgatatc
//
LOCUS       DAOMAA010001041         1017 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_210980_length_1017_cov_3.349272, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010001041 DAOMAA010000000
VERSION     DAOMAA010001041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_210980_length_1017_cov_3.349272"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1009)
                     /locus_tag="PLO59_11695"
     CDS             complement(<1..1009)
                     /locus_tag="PLO59_11695"
                     /inference="COORDINATES: protein motif:HMM:NF012951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HQV01820.1"
                     /translation="MQNSLSQPASEVNNLLNNNTPYVIRLKVMPDNEVAFDDLVRGHV
                     VFNTNIVDDKVLLKSDGMPTYHLAHIVDDYLMQITHAVRGEEWLPSAPAHILIYKYLG
                     WESKMPHYAHLPLLLKPDGHGKLSKRDGDRLGFPVFPLNWTDPITAEISSGYRERGYL
                     PQAFVNMLALLGWNSGTTQELFTMPQLINAFSFKHIHKAGAKFDPEKAKWFNEQYVRA
                     LPDATLVDHLRNDVIESYKYSGDDVRLTDSYLIKAVQLLKPRIQFLHDMHSMGHYLFE
                     PPQTYDTDALQKKWNENQFAFYDVLLPQLNTQQSFDATVIEPLVKNLIKEMGLKMGDV
                     IS"
BASE COUNT          281 a          209 c          211 g          316 t
ORIGIN      
        1 atgaaattac atcgcccatt tttaagccca tttcttttat taaatttttt accagtggct
       61 cgataacggt tgcatcaaaa ctttgttgtg tatttaattg cggcagtaat acatcataaa
      121 aagcaaactg attttcattc catttttttt gcaatgcatc tgtatcgtac gtttgtggcg
      181 gttcaaaaag gtagtggccc atggagtgca tatcgtgcaa aaactgaatg cgtggtttaa
      241 gtaactgtac ggcttttata aggtagctgt ctgttaatct aacatcatca ccactatact
      301 tataactttc aattacatcg ttacgtaaat gatcaactaa tgttgcatcg ggtagtgcgc
      361 gcacatattg ttcgttaaac cacttggctt tttcggggtc gaattttgca cctgctttat
      421 gaatatgctt aaagctaaag gcattaataa gttggggcat ggtaaacaac tcttgtgtgg
      481 tgccactgtt ccaacctaaa agtgccaaca tatttacaaa tgcctgtggc aggtaaccac
      541 gctcgcggta gcctgacgaa atttcggctg taatggggtc ggtccagtta agcggaaaca
      601 ccggaaaacc cagcctgtcg ccatcgcgct tgcttaattt tccatggcca tcgggcttga
      661 gtaaaagcgg caagtgtgca taatgcggca ttttactttc ccagcctaag tacttgtaaa
      721 ttaaaatatg tgccggtgca ctgggcaacc actcttcgcc acgtacggca tgtgtaattt
      781 gcattaaata atcgtcaact atatgcgcta agtgataggt aggcatgcca tcgcttttca
      841 gcaaaacttt atcgtcaaca atattggtat taaacaccac atggccacgc accaaatcat
      901 caaaagcaac ttcgttatcg ggcatcactt taaggcggat aacataaggt gtattgttat
      961 tcaaaaggtt gtttacttct gatgcgggct ggcttaatga gttttgcatt tgcatgc
//
LOCUS       DAOMAA010001042         1017 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_211156_length_1017_cov_2.034304, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010001042 DAOMAA010000000
VERSION     DAOMAA010001042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_211156_length_1017_cov_2.034304"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="PLO59_11700"
     CDS             complement(<1..277)
                     /locus_tag="PLO59_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HQV01821.1"
                     /translation="MSLSIQDAQMQVDQWIKTHGVRYFNELTNMALLTEEVGEMARII
                     ARKYGEQSYKTTDANLNLADEMADVLFVLICLANQTQINLEEAFKQNI"
     gene            405..>1017
                     /locus_tag="PLO59_11705"
     CDS             405..>1017
                     /locus_tag="PLO59_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HQV01822.1"
                     /translation="MHYKEFKQLDLAAIHKELLHVWQNENTFEKSIELRNGCKPFTFY
                     EGPPSANGLPGIHHVMARSIKDIFCRYKTLQGYQVKRKGGWDTHGLPIEISVEKTLGI
                     TKEDIGTKISVEDYNKACRTEVMKYKDVWDDLTVKMGYWVDLQNPYVTFENEYIESVW
                     HLLQMLYDKKLLYKGYTIQPYSPAAGTGLSSHELNQPGTYRNVR"
BASE COUNT          327 a          209 c          183 g          298 t
ORIGIN      
        1 caatattttg tttaaacgcc tcctccaaat ttatctgtgt ttgatttgcc aaacaaataa
       61 gcacaaaaag cacatcggcc atttcatcag ccaaattcag atttgcatca gttgttttgt
      121 atgactgctc gccatatttc cgggcaataa ttcgtgccat ttcgcccact tcttcggtta
      181 acaaagccat attggtaagt tcgttaaagt agcgtacacc atgggtttta atccattggt
      241 caacctgcat ttgcgcatct tgaattgata atgacatctc atataatttt tgccaaagta
      301 acttccaaaa tcaaattgtt caatataaat gtgggattgc tactttcgcc accccaaaaa
      361 cgcctgagct gctaaatagc tgccggtttt acagaatctg ttttatgcac tacaaagaat
      421 ttaaacaact cgaccttgcc gctatacata aagaactttt acatgtatgg caaaacgaaa
      481 acacttttga aaaaagcatt gaattacgca atggttgtaa accatttacc ttttacgaag
      541 gaccgccttc ggccaatggt ttaccgggta ttcatcatgt aatggctcgc agtattaaag
      601 atattttttg ccgttataaa acattacaag ggtatcaggt aaaacgcaaa ggcggatggg
      661 atacacatgg attacctatt gaaattagtg tcgaaaaaac cttaggcata accaaagaag
      721 atattggcac aaaaatatcc gttgaagact ataacaaagc ttgccgcact gaggtaatga
      781 aatataaaga tgtttgggac gaccttaccg ttaaaatggg ctattgggtt gatttgcaaa
      841 atccttacgt aaccttcgaa aacgaataca ttgaatcggt ttggcatctg ttgcaaatgc
      901 tttatgataa aaaactgctt tataaaggtt ataccataca accttattca ccggctgcgg
      961 gtacaggcct tagttcgcat gaattaaacc aacccggcac ctaccgcaat gtacgcg
//
LOCUS       DAOMAA010001043         1016 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_211560_length_1016_cov_1.981270, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010001043 DAOMAA010000000
VERSION     DAOMAA010001043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_211560_length_1016_cov_1.981270"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1016)
                     /locus_tag="PLO59_11710"
     CDS             complement(<1..>1016)
                     /locus_tag="PLO59_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01823.1"
                     /translation="MDQIKTFPAARVKKGNTLLRNGTLTNHAYTGGRFRPGINDATAV
                     RNSVEIQKQLSYYFNSAIQVSGATTEWAQFGDTTKFTGAWINFANQNPTIPTTISTSW
                     PHVIPTMAGFTSNENYVTCRCLPNSSYLQNSAGQFIDNNGVVTPWKRLAPDSPLDSLI
                     QDGLTQKFYFTKLFKELKRPLDFILDEAENLPFYRSVHTGPTLDASVVSNKNASGLDW
                     VTYFSRRYAVQAKAYRDNFMNLPQLANTKYQLYETEGHDDYRPKYTESRTINSQTNGT
                     YYPVASMYTRFPSNWRYNSAAWNGWQWFAESRVNEIAAGDNYCAPAVAAGWDDNEENN
                     VRPA"
BASE COUNT          276 a          203 c          206 g          331 t
ORIGIN      
        1 tgtgctggtc ttacattgtt ttcttcattg tcgtcccaac cagcggcaac agcgggcgca
       61 caataattat cacctgcggc aatttcgttc acgcgcgatt cggcaaacca ttgccatcca
      121 ttccatgcag ccgaattgta gcgccaattt gaaggaaacc gggtgtacat acttgctacc
      181 gggtaataag tgccattagt ttgtgagttt atcgttctcg actcagtgta tttagggcga
      241 taatcgtcat ggccttcggt ttcatataac tgatatttag tgtttgccag ttgtggcaaa
      301 ttcataaagt tatcacggta tgctttggct tgtactgcat aacgtctgct aaaataagtt
      361 acccaatcta aaccacttgc gtttttgttt gatacaacag aagcatctaa ggttggccca
      421 gtgtgtacgg aacgatagaa tggtaaattt tcggcttcat ctaaaatgaa atcaagtggc
      481 cgttttaatt ctttaaacag ctttgtgaaa taaaactttt gtgttaatcc atcctgaatt
      541 aatgaatcta aaggtgaatc tggtgctaat cgtttccaag gcgttacaac tccattgtta
      601 tcaataaact gacccgcact attttgtaaa taagaactat tgggcaaaca acggcaggtt
      661 acatagtttt cgttcgaagt aaaacctgcc attgttggta taacatgtgg ccatgatgta
      721 ctaatagtgg ttggaattgt agggttttga tttgcaaaat ttatccatgc accggtaaac
      781 tttgttgtat cgccaaactg cgcccattct gttgtggcgc cactgacttg aattgctgag
      841 ttgaaataat aagataattg tttttgtatt tccacactat tacgtacagc agtagcatcg
      901 ttaatacctg gcctgaaacg cccacctgtg tatgcatgat tagttaacgt accatttctt
      961 aaaagtgtat tgcctttttt aacccgagca gccggaaacg tttttatctg atccat
//
LOCUS       DAOMAA010001044         1015 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_211883_length_1015_cov_2.215625, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010001044 DAOMAA010000000
VERSION     DAOMAA010001044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_211883_length_1015_cov_2.215625"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /locus_tag="PLO59_11715"
     CDS             complement(<1..980)
                     /locus_tag="PLO59_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01824.1"
                     /translation="MVSPGPGGVLEPVKGAAKTNPGTYECKTSGIQNATQNLLLKKHS
                     VTRIYVTKAKVKLIATDTSICSGTTTSVVARGGSGYTYNNGSLTSMKADNSIMQFTAP
                     VVSTPTNYRIIVTTSQGYKDTVDITVNPKPVLTVSASATTVCKGAPVNLTATLTGGNA
                     GNTKSFLWYPSQYFVKADTAKAVAMPTKDITFKCFATDGVCFALSDSVPTVTVIPNAN
                     AGSDVAVCDDSLPVLLKANFINGASTYKWYDENGVLLNNGTTVNVSPTAPTTYRLIVT
                     QGVSACKDTDYVQVKPFTCCASAEYPITLTIRPGEKWVENVVPRLMAFCLA"
BASE COUNT          288 a          197 c          208 g          322 t
ORIGIN      
        1 gccaaacaaa atgccatgag acgtggcaca acattttcga cccatttttc gcccggcctt
       61 atggtaagtg ttattggata ctcagcactt gcacaacagg taaaaggctt aacctgaaca
      121 taatcagtgt ctttacaagc actaacgcct tgtgtaacta ttagtctgta tgtagttggt
      181 gcggttgggc ttacgttaac agtggtaccg ttatttagta atacaccatt ttcatcatac
      241 catttatagg ttgaagcacc atttataaaa ttggctttaa gcaaaacggg taaactatcg
      301 tcacaaacag caacatcaga accggcattt gcatttggta ttactgtaac agttggtact
      361 gaatcgctta atgcgaaaca cacaccatca gttgcaaaac atttaaaagt aatatccttt
      421 gttggcattg ccactgcttt agcggtatcg gcctttacaa aatattgtga tggataccat
      481 aaaaaagatt tggtattacc ggcattacct cctgttaagg tggcggttaa gtttacagga
      541 gcgcctttac aaacggttgt tgctgatgca cttactgtaa gtaccggctt tgggtttact
      601 gtaatatcaa cggtgtcctt ataaccttgc gatgtggtaa ctattatacg atagtttgta
      661 ggtgttgaaa caaccggtgc tgtaaactgc attattgagt tatcagcttt cattgaagtt
      721 aacgagccat tgttataagt ataacctgaa ccgccacgtg ctacaacact ggtagttgta
      781 ccactgcaaa ttgaagtatc ggtagcaatt aatttaacct ttgcttttgt aacatatata
      841 cgggttacgg agtgtttttt aagaagcagg ttttgggtgg cattttgtat gcctgatgtt
      901 ttacattcgt atgtacccgg atttgttttg gcagctcctt ttacgggttc taaaacacca
      961 ccgggtcctg gcgaaaccat gatatattta ccaccgctaa cattaaaaac attgg
//
LOCUS       DAOMAA010001045         1014 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_212290_length_1014_cov_2.278415, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010001045 DAOMAA010000000
VERSION     DAOMAA010001045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_212290_length_1014_cov_2.278415"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..867
                     /locus_tag="PLO59_11720"
     CDS             <1..867
                     /locus_tag="PLO59_11720"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HQV01825.1"
                     /translation="TRIITYQAVLCCLLNMALAYVLIFGKCGFQPMGIKGAAIANAVS
                     EIIAALYIILWAMLRKPFKAYGLFKFARFNKIAAFKIVEISLPIMLQHFLSMAAWFVF
                     FVLIEKMGRHPLAISNIIRSCYMVMMTPVWGFAAASNSMISNLIGQGRKQDVLPVLFK
                     IIKLSLFFILILVAVLACFPTLLLKLSTNDSLLIADSMHCFYITLAATIIFSISMNLL
                     SAVSGTGATRSALIIELTNITIYTLYIFCFTVIVKSTVEVVWLSEIVYWTSMGIWSYY
                     YLIKGKWKNLAV"
     gene            884..>1014
                     /locus_tag="PLO59_11725"
     CDS             884..>1014
                     /locus_tag="PLO59_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HQV01826.1"
                     /translation="MKNMRILFLGTPEFAVGILKQLLSNKCNVVAVVTAADQPAGRG"
BASE COUNT          278 a          163 c          206 g          367 t
ORIGIN      
        1 acgcgcataa ttacttatca ggctgtttta tgttgtttgc ttaacatggc attggcctac
       61 gtactcatat ttggtaaatg tggtttccaa cccatgggta ttaaaggggc agctatagct
      121 aatgccgttt ccgaaattat tgccgcacta tatataattt tatgggctat gttgcgcaaa
      181 ccgtttaaag cttatggctt atttaagttt gcgagattta acaagatagc tgcttttaaa
      241 attgtagaaa ttagtttgcc cattatgctg caacattttt tatcaatggc tgcatggttt
      301 gtgttttttg tgttgattga aaaaatgggt cgtcatcctt tagctatctc taatattata
      361 cgtagttgtt acatggttat gatgacaccg gtatggggct ttgcagcggc cagcaatagt
      421 atgataagca acttgatagg gcagggacgt aagcaagacg tattgcctgt actttttaaa
      481 attattaaac tgagcttgtt ttttattttg attttagtag ctgtattggc ttgctttcca
      541 acgcttttgc ttaaacttag taccaacgat agtttactta ttgccgactc catgcattgt
      601 ttttatatca cactggcagc aacaattatt tttagtatat caatgaattt attaagtgcc
      661 gtatcgggta ccggtgcaac acggtcggca cttataatcg agttaaccaa cattaccatt
      721 tatacattat atattttttg ctttacagta attgtaaaaa gtaccgttga agttgtttgg
      781 cttagcgaaa ttgtttattg gacttctatg ggcatttgga gttattatta tttgataaaa
      841 ggcaaatgga aaaacctggc tgtgtaattt gaactttatg gagataaaaa atatgcgcat
      901 tctttttttg ggcacacctg aatttgcagt cggtatttta aaacagttgc tcagcaataa
      961 atgcaatgta gtagctgttg taactgcagc cgaccaacct gcaggccgtg gatt
//
LOCUS       DAOMAA010001046         1011 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_213405_length_1011_cov_3.254184, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010001046 DAOMAA010000000
VERSION     DAOMAA010001046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_213405_length_1011_cov_3.254184"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1011
                     /locus_tag="PLO59_11730"
     CDS             <1..>1011
                     /locus_tag="PLO59_11730"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HQV01827.1"
                     /translation="TGSFTYIAPKIKSITVTGAQTLDVLFTENADAITAQNPNNYAAN
                     NGLANPVTAVLDATNGALVHLTFANSFVDGLTNTLTVTNILTANGWPLLPNNQVPFIY
                     YAPVVVVKGDIVINEIMAAPSGTLGLPNFEYVELFNASNKTIDLNGFTFTDASGSSAP
                     FNSYILMPQSYVIICSSTAAAALSSFGNTLAITSFPSLNNTGDDLTLLNLFGNSIDVV
                     SYTDAWYNNVSITSGYALEKIEATYPCQLQANWAASQAAIGGTPGQTNSVAGVFTDTL
                     PPAIATVSVGGNNVMVLFFSEPVTFASASILSNYHVSNGVGNPVTVTVSTYTVTLTFA
                     NPW"
BASE COUNT          305 a          215 c          199 g          292 t
ORIGIN      
        1 accggcagct ttacgtatat agcacctaaa ataaaaagta taacagttac aggtgcccaa
       61 acgttagatg tgttatttac cgaaaatgca gatgccatta ccgcacaaaa cccaaacaac
      121 tacgcggcta ataatgggtt agcaaacccg gttacggccg tgttagatgc aacaaacgga
      181 gctttggtac accttacctt tgcaaatagt tttgtagatg gacttaccaa tacactaact
      241 gttacaaata tacttacagc caatggttgg ccattattac ccaacaacca agtgccattt
      301 atttactatg caccggttgt ggtagttaaa ggtgatattg ttattaacga aataatggct
      361 gcaccatcgg gcacattagg gttacctaac tttgaatatg tcgaactgtt taatgccagc
      421 aataaaacca ttgatttaaa tggctttact tttaccgatg ccagtggcag cagtgcccca
      481 tttaacagct atatactaat gccacaatcg tatgtaatta tttgcagcag taccgctgcc
      541 gcagcattaa gcagttttgg taatacgctg gctataacct cttttccaag tttaaataat
      601 acaggcgatg acttaacttt attaaatctt tttggtaata gtattgacgt tgtttcttat
      661 accgatgcct ggtataacaa tgtaagcata accagtggct acgctttaga aaaaatagaa
      721 gccacctatc cctgtcagtt gcaggccaat tgggcggcaa gccaggcagc aattggaggt
      781 acacctggcc aaaccaatag cgttgccgga gtttttaccg atacacttcc gcctgctatt
      841 gcaacggtta gcgtgggagg taacaatgta atggtgttgt tttttagcga acctgtaact
      901 tttgcttcgg caagtatttt atcaaactac catgtaagca atggtgtagg taatcctgta
      961 acagttactg taagcacata caccgtaacc ttaacttttg caaacccctg g
//
LOCUS       DAOMAA010001047         1011 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_213445_length_1011_cov_2.796025, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010001047 DAOMAA010000000
VERSION     DAOMAA010001047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_213445_length_1011_cov_2.796025"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1011
                     /locus_tag="PLO59_11735"
     CDS             <1..>1011
                     /locus_tag="PLO59_11735"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HQV01828.1"
                     /translation="NTEDINYPAGYANVFSIASTNSSDGKSSFSSYGATIDMCAPGSG
                     IQSTVYNNSYTQMSGTSMASPIAAGCAALVKSQFPNYTGQQVGEQLRVTCDNIYGVGI
                     NSQYLNKLGTGRINLFEALTNTTTPSIRFTHQLATDNNDNTFVNGDTVRLSGVHKNFL
                     APTTNLTATLSTTNPIVTILNNTINLGVVNTLDSVNNVANPFTFKINATAGLNAKVQF
                     KLTYNDGTYQDVEMIIMTVNVDYINFSVNNIATTVTSKGRIGFNNDNNADGQGFMYDG
                     ENLMYDGGLMIGNSSSAVSDVIRGTGSGNDADFQSINRIQQLLPAATSNFDTYGFMSD
                     VSS"
BASE COUNT          340 a          197 c          192 g          282 t
ORIGIN      
        1 aatacagaag atataaacta tccggccggc tatgccaatg tgttttcaat tgccagcaca
       61 aattcatctg acggcaaatc gtcatttagt agttatggtg caactataga tatgtgtgca
      121 cccggctcag gtattcaatc tacagtgtat aacaacagct acacacaaat gagtggcaca
      181 tcaatggcat cacccattgc tgcaggatgt gcagcgcttg taaaatcaca attccccaat
      241 tacaccgggc agcaagtggg cgagcaattg cgcgtaacct gcgataatat ttatggtgta
      301 ggtataaata gtcaatacct taataaatta ggcacaggcc gtattaattt attcgaagca
      361 ttaaccaata caaccacgcc atcaatcagg tttacacatc aattagcaac cgataacaac
      421 gacaatacat tcgttaatgg cgataccgtt cgcttgagtg gtgtacataa aaacttttta
      481 gctcctacta ccaatctaac agcaacatta agtactacaa accctattgt aaccatactt
      541 aacaatacta taaacttagg agttgtaaat acactcgact cagtaaacaa tgttgccaat
      601 ccgtttactt ttaaaataaa tgccaccgct ggtttaaatg ctaaagtgca gtttaagcta
      661 acttataacg atggcacata tcaagatgta gagatgataa ttatgactgt aaatgtggat
      721 tatataaact ttagtgtaaa caatattgcc accacagtta ccagtaaagg acgcattggt
      781 tttaataatg ataacaatgc cgatgggcaa ggctttatgt atgatggcga aaatttaatg
      841 tatgatggtg gattaatgat tggaaatagc agtagtgccg ttagcgatgt aatacgtggt
      901 acaggcagtg gcaacgatgc cgactttcaa tccattaacc gcatacaaca attgttacct
      961 gctgcaacaa gcaattttga tacgtatggg tttatgagtg atgtatcgtc a
//
LOCUS       DAOMAA010001048         1011 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_213485_length_1011_cov_2.535565, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010001048 DAOMAA010000000
VERSION     DAOMAA010001048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_213485_length_1011_cov_2.535565"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="PLO59_11740"
     CDS             <1..487
                     /locus_tag="PLO59_11740"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HQV01829.1"
                     /translation="SNAAGGMNKNFQVGDLMIIEDHINLMPSNPLIGKNDDNLGPRFP
                     DMSVPYNKEMIARAKVIADEMGHRCHIGVYAAVSGPCFETPAEYKYLRIIGADVVGMS
                     TVPEVIAAKHCGMNCFAMSIVTDLGGFDDVQSVSHEEVLKVANEAEVKMSAIVAQLLN
                     S"
     gene            518..>1011
                     /locus_tag="PLO59_11745"
     CDS             518..>1011
                     /locus_tag="PLO59_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01830.1"
                     /translation="MKKLTLILMLFTSIAYAQYPSQNVTLLSTWDDITVPAESYYGIR
                     YNGIWGWTSPNGMEYAIIGATTGTYIINVSNPATPVVCDFVPGCRVDCIWRELKTYQN
                     FLFMVSDDSPPNCLQVADLSYLPDSVHVVHESDNIIETAHTIFIDDHYLYAASPRGGV
                     VGNSA"
BASE COUNT          313 a          198 c          223 g          277 t
ORIGIN      
        1 gtcgaatgct gcgggtggca tgaataaaaa ttttcaggtg ggcgatctta tgatcattga
       61 agaccacata aatttaatgc catcaaatcc attgatagga aaaaatgacg acaatcttgg
      121 cccacgcttt cccgatatga gtgtgcctta caacaaagaa atgattgcgc gtgcaaaagt
      181 tattgccgat gaaatgggac atcgctgcca cataggtgta tacgcagctg tgtcgggtcc
      241 atgtttcgaa acccctgccg aatataaata tctgcgcatt ataggtgccg atgttgtagg
      301 catgagtacc gtacccgaag taatagcagc aaaacattgc ggcatgaatt gctttgccat
      361 gagcattgta accgaccttg gcggttttga tgatgtacaa agtgtgagtc acgaagaagt
      421 actaaaagtt gccaacgagg ccgaagtaaa aatgagtgct atagttgcac aattattaaa
      481 cagttaaaaa ttaaatctaa aaataaaata atctaaaatg aaaaaactaa cattaatttt
      541 aatgctcttt acaagcattg cttatgcgca atatccttcg caaaatgtaa cattgctttc
      601 aacctgggac gacatcacag tgccggccga atcctattat ggcataaggt ataatggtat
      661 atggggctgg acatcaccca atgggatgga gtatgctatc ataggagcaa ctacaggtac
      721 gtatataatc aacgttagca atccagccac accggtagtt tgcgattttg tacccggatg
      781 ccgtgtggat tgcatttggc gtgaattaaa aacttaccag aattttttat tcatggtaag
      841 tgacgatagc ccaccgaatt gcctacaagt agccgatttg agttacctcc ccgattcggt
      901 gcatgtggtg catgaaagcg ataatataat tgaaacagct cataccattt ttattgacga
      961 ccactatttg tatgctgcca gtccacgcgg tggcgttgtt ggtaattctg c
//
LOCUS       DAOMAA010001049         1007 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_215003_length_1007_cov_3.912815, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010001049 DAOMAA010000000
VERSION     DAOMAA010001049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_215003_length_1007_cov_3.912815"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1007)
                     /locus_tag="PLO59_11750"
     CDS             complement(<1..>1007)
                     /locus_tag="PLO59_11750"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HQV01831.1"
                     /translation="SNITCAFDYIELGAPPVDDAIFWYYDGSTWAVLINTPSTNNSGC
                     GSAGRWTNYTIALPASANNNPAIKLGIQWINNDDGIGTDPSFAIDNLQLYITQAPLPL
                     ASFAASNVSFCDSVCISFADSSINSPTSWQWVFTGANPATATVQSPTNICYNVPGTYD
                     VTLICSNAAGADTLTLTNYITVNPCHMPVVQFYASDTLLCEESCISFFDLSTNNPTQW
                     FWTFNGAMQPDTSSLQNPTLICYNTVGTYTVTLTATNAQGSTTATYTSYITVNPNPPT
                     PIISLNGQVLVSTPAYSYQWYLGALPLIGETNQVLFAPTPGDYYVIVTDSNGCFSISN
                     T"
BASE COUNT          306 a          195 c          222 g          284 t
ORIGIN      
        1 atggtgtttg aaatagaaaa gcaaccatta ctatcggtaa caatcacata ataatcgccc
       61 ggtgtgggtg caaataaaac ctggttggtt tcgccaatta atggtaaagc acctaaatac
      121 cattgatagg agtaggcagg tgtgctaact aaaacttgtc cgtttaacga aataatgggt
      181 gttggcggat tagggtttac cgtaatatat gaagtatagg ttgctgttgt acttccctgg
      241 gcattggttg cagttaatgt tactgtatat gtacccactg tattgtaaca aattaacgta
      301 ggattttgta atgaactcgt atctggttgc atggcgccat taaatgtcca aaaccattgt
      361 gtagggttgt tggtgcttaa atcaaaaaaa ctgatacaac tttcttcaca caacaatgta
      421 tcgcttgcat aaaattgcac cacaggcata tggcaaggat taaccgtaat ataattggta
      481 agggtaagcg tatcggctcc ggcagcgttg ctacaaatta gtgtaacatc ataagtgccc
      541 ggtacgttat aacaaatatt tgttgggctt tgaaccgtag ctgttgccgg atttgcacct
      601 gtaaataccc actgccagct ggttggacta ttaattgagc tatcggcaaa cgaaatacaa
      661 accgaatcgc aaaaactcac attgcttgcc gcaaacgatg ccaatggcaa aggtgcctgc
      721 gtaatgtata attgcaagtt gtcaattgca aatgaagggt cggtacctat gccatcatca
      781 ttatttatcc attgaatacc taatttaata gcggggttat tatttgccga agccggcaag
      841 gcaatggtgt aattagtcca acggcctgcc gaaccgcagc ccgaattgtt ggttgaaggt
      901 gtgttaatca acacagccca ggtactgcca tcatagtacc aaaatatagc atcatcaacc
      961 ggtggtgcac ccagttcgat ataatcaaat gcacaagtaa tgttgct
//
LOCUS       DAOMAA010001050         1007 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_215289_length_1007_cov_1.606092, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010001050 DAOMAA010000000
VERSION     DAOMAA010001050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_215289_length_1007_cov_1.606092"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="PLO59_11755"
     CDS             complement(<1..461)
                     /locus_tag="PLO59_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQV01832.1"
                     /translation="MKNIFIRVIVSALLLSGLSVNGQTTSDSTKTTPKKYQAKATATQ
                     PFGVKAFSKSKKTTLRWLGMAGFLINSRGTTFMVDPLLEGFDMPMMIDFPILAKNVPQ
                     LDAVFVTHADNDHFSVPTNVAMSKVTDVYHTTVYVDSLMKNLSLKSIGHIIG"
     gene            complement(647..>1007)
                     /locus_tag="PLO59_11760"
     CDS             complement(647..>1007)
                     /locus_tag="PLO59_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQV01833.1"
                     /translation="VYGNMYNKYLVELYTGILNDPAKAAATAKKDIANRATPQTYAWY
                     VWALYCNNKTAEAEKIYQQYVSGKPLEGLELYWMGKYMHGQGKGYNAQQFFKAAYKNR
                     YDLSLAMVRDLEKDLEE"
BASE COUNT          287 a          209 c          165 g          346 t
ORIGIN      
        1 ccaatgatgt gtccaataga tttgaggctt aagtttttca tcaatgaatc aacataaact
       61 gtcgtatggt aaacatctgt aacttttgac attgctacat ttgtcggaac actaaaatgg
      121 tcattgtcgg catgggttac aaaaacagca tctaactgag gtacattttt tgccaaaatt
      181 ggaaagtcaa tcatcattgg catatcaaaa ccttcaagta atgggtcaac cataaaagtt
      241 gtaccacgac tattgattaa aaaccctgcc attcctaacc accgcaatgt tgtttttttt
      301 gacttgctaa atgcttttac accaaatggc tgtgtcgctg tagcttttgc ctgatatttt
      361 ttaggtgtag tttttgtact gtcggaagtt gtttgtccat tcacacttaa ccctgatagt
      421 aaaagtgcag aaactatcac tctgataaaa atatttttca tttgtcgatt ttattggttg
      481 ttattttatg tcctgtgtcc ataaagttaa ttgtaagtag ggtgccgata aaattacgcc
      541 caactcataa aattacgcaa ctccttgtat agtttacaac tgattatcaa tataaaacta
      601 tacaaaagaa ttcctgttac ccgcaatatt tttgaaaata aaaatgctac tcttccaaat
      661 ccttctccag atccctcacc atcgccaggc ttaaatcata ccggttctta tacgctgctt
      721 taaaaaactg ctgtgcatta taacccttgc cctgcccatg catgtatttt cccatccagt
      781 aaagttcaag cccctccaat ggcttacccg aaacgtattg ctgatatatc ttttcagctt
      841 cggctgtttt attgttacag tataatgccc atacatacca ggcataggtt tgtggcgttg
      901 cccggttggc tatatctttt tttgcagttg ctgctgcttt tgcaggatcg tttaaaatac
      961 ccgtgtatag ttcaaccagg tatttgttat acatattgcc atagacc
//
LOCUS       DAOMAA010001051         1006 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_215523_length_1006_cov_2.315457, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010001051 DAOMAA010000000
VERSION     DAOMAA010001051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_215523_length_1006_cov_2.315457"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1006
                     /locus_tag="PLO59_11765"
     CDS             <1..>1006
                     /locus_tag="PLO59_11765"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQV01834.1"
                     /translation="AKLRNLQIELDNINMLSSLLDDDDQSNLAAIANILQTQNNPGLL
                     SAFDKLQRLNEQRQALLFNQTPSSPAVKDVDALIATVKSTINGTVINIRKTLAAQINS
                     LRSNIGQINQKVSSTAYVNRGLQNVKRSVDLNERMFLFLMETRAQTMIARSAIVADKF
                     ILEPARSQGLVHPLPSKVLITGIGIGLAFAFLVIFFKNLYLNYIITKDDLKEITHLPI
                     VGIVAKAKEGEKEYNMVDKYPQSPASEAFRVIRTNLSYYKTRVVVFTSSVAGEGKTFC
                     AINTATILAKSKKKVLIIDCDLHKPKQANAFNLNNDIGITSYLAGKNDINSIIKPTGI
                     E"
BASE COUNT          355 a          191 c          184 g          276 t
ORIGIN      
        1 tgccaaactg cgaaatttac aaattgagtt agataacatc aacatgctta gcagcttgtt
       61 agatgatgat gaccaaagta atttggctgc tattgccaac atattacaaa cacaaaacaa
      121 tccggggtta ttaagcgcct tcgacaaatt acaacgatta aatgagcaac gccaagcctt
      181 actttttaat caaaccccaa gcagcccggc cgttaaagat gttgatgcat taattgctac
      241 cgtaaaaagt actattaacg gcaccgttat taatatccgt aaaacacttg ctgcacaaat
      301 aaacagtttg cgatctaaca tcggtcagat aaatcaaaag gtaagcagta ctgcttatgt
      361 aaaccgtgga ttacaaaatg taaaacgttc ggtagattta aatgagcgca tgtttttatt
      421 cttaatggaa acacgcgcac aaaccatgat agcacgcagt gctattgttg ccgataaatt
      481 tattttagag cctgcacgct cgcaaggctt agtgcatcca ttaccatcta aagttctaat
      541 tacgggtata ggcattggtt tggcctttgc atttttggta atattcttta aaaacttata
      601 tctcaactac atcatcacca aagatgattt aaaagaaata acacacctgc caattgtagg
      661 tattgtagcc aaagctaaag aaggcgagaa agaatacaac atggttgaca agtatccgca
      721 aagtccggca tcggaggcct ttagggtaat cagaacaaac ttaagttatt ataaaacccg
      781 tgtggtagta tttacatcga gcgttgcggg cgaaggcaaa acattttgtg caataaacac
      841 ggcaaccatt ttagccaaga gtaaaaagaa agtattaatt attgactgcg atttgcataa
      901 gcctaaacaa gccaatgcat ttaatctgaa taatgatata ggtattactt cttacctggc
      961 cggcaaaaat gatattaata gtattattaa accaacaggc atcgaa
//
LOCUS       DAOMAA010001052         1005 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_215839_length_1005_cov_3.171579, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010001052 DAOMAA010000000
VERSION     DAOMAA010001052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_215839_length_1005_cov_3.171579"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            335..>1005
                     /locus_tag="PLO59_11770"
     CDS             335..>1005
                     /locus_tag="PLO59_11770"
                     /inference="COORDINATES: protein motif:HMM:NF019139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HQV01835.1"
                     /translation="MYDILELSDKSLDQLKTIATEIGLSNLGEIEKQKLIYKILDQQA
                     LQPITTDESKKKTRKPKTTKTKAAIAADTLTTTPANTDAETDFKPVETIAEPVATYNT
                     ETETPIPLQATAHQNANDTDKPKRQRIRIQIEEEDTNKPAATVTTATQTNTTETENKP
                     VVETTPNAPVINPQKSSETISNPTEFSKPAEPRNNENRQQFQPRHQNEHRQNNRDDRP
                     REFRQ"
BASE COUNT          392 a          242 c          133 g          238 t
ORIGIN      
        1 ttgcgcacca aaatgtttac cttttcgcta ttgttcgaat aaccgttttg cttacaagtt
       61 ataccattgc atttaaaaac ttaccaaact taatttataa cctaaccgtt ataatccaaa
      121 taaaaaactt gtttggtatg tagttgtatg ttatcattgc agcggcttaa cacttatttc
      181 tgtttcaaca tataaagcag ttaagcccca aacctttaag gttcatatca aatccacact
      241 ttttattcct tactaaagct acaactttta aacatcacgt tttttttgat ttatatcaaa
      301 aaaaactttc gttataagca tttaaaacaa taacatgtac gatattttag aactcagcga
      361 taaatcgctt gaccaattaa aaaccattgc caccgaaatt ggcttatcta acctgggcga
      421 aatcgaaaaa caaaaactca tttataaaat tttagaccaa caagctttgc agcccataac
      481 caccgatgag tcgaagaaaa aaacaagaaa acccaaaacc acaaaaacca aagccgcaat
      541 tgctgccgat accctaacta caacaccggc aaatacagat gctgaaactg attttaagcc
      601 cgttgaaacc attgccgagc ctgttgcaac ttataacaca gaaaccgaaa cgcctatacc
      661 ccttcaagca acagcacatc aaaacgctaa cgataccgat aaaccaaaac gccaacgtat
      721 aaggatacag atagaagaag aggatactaa caaacctgcc gctactgtaa ctacagcaac
      781 acaaactaac acaactgaaa ccgaaaataa accagttgtt gaaacaacac caaatgcacc
      841 tgtaattaac ccgcaaaaat catcagaaac tatatctaac ccaactgaat tttcgaaacc
      901 ggccgaaccc cgtaacaacg aaaacagaca gcaatttcag ccccgccatc aaaatgagca
      961 ccgccaaaat aatagggacg acaggccacg cgaattcaga caaga
//
LOCUS       DAOMAA010001053         1005 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_215870_length_1005_cov_2.766316, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010001053 DAOMAA010000000
VERSION     DAOMAA010001053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_215870_length_1005_cov_2.766316"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="PLO59_11775"
     CDS             complement(<1..445)
                     /locus_tag="PLO59_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010424269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HQV01836.1"
                     /translation="MQNRYSQRGVSADKEDVHQAIKAVDKGLYPQAFCKIVSDTLGND
                     ANWCNIMHADGAGTKSSLAYAYWKETSDLNVWADIAKDALVMNIDDLLCVGATQNILL
                     SSTIGRNKNLIPGDVIAAIINGTEQCVNELNHLGLQIHTTGGETAD"
     gene            complement(535..>1005)
                     /locus_tag="PLO59_11780"
     CDS             complement(535..>1005)
                     /locus_tag="PLO59_11780"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HQV01837.1"
                     /translation="SKDFDFPLKSTARPIDLPEVFYDLNKATLRPESSTALDGLVKTL
                     DENPTITILITANTDFRADDKYNKNLSDRRAKSVYDYLIKKGVPADRLQSEGKGESEP
                     KVIGKDFADYPPFKEGDVLTEEFIKKLSPEDFEKANQYNRRTDFRVLRTDYVPH"
BASE COUNT          264 a          200 c          190 g          351 t
ORIGIN      
        1 catcggcagt ttcgccaccg gtggtatgta tttgcaagcc caaatggttt aactcattta
       61 cacactgttc agtgccattt ataatggctg ctattacatc gcccggtatc aggtttttat
      121 tgcggcctat ggttgatgat aataatatgt tttgagtagc gcccacacat aataagtcat
      181 caatattcat aaccaaagca tcttttgcaa tatctgccca aacgtttaag tcgctggttt
      241 ctttccaata agcataagct aatgatgatt tggtacctgc accatcggca tgcataatgt
      301 tgcaccagtt ggcatcgttg cctaatgtat cggatactat tttgcaaaat gcttgtggat
      361 acaatccttt gtcaacggct tttattgcct gatgaacatc ttccttatcg gcacttacac
      421 cccgttgcga atacctgttt tgcatgaaaa ttttgtttga attattgcta aggatttgcc
      481 taaaaaaata cgagcacctg tgtaggtgct cgtatttttt tatcttataa tttattagtg
      541 tggcacataa tcggtacgta atacacggaa atcggttcta cgattgtact gattggcttt
      601 ttcaaaatct tcaggactca gttttttaat aaactcctct gttaacacat cgccttcttt
      661 aaaaggtggg tagtcggcaa aatctttacc aataactttt ggttcgcttt cgcctttacc
      721 ttccgattgt aaacgatcgg ccggtacacc tttcttaatt aagtaatcgt aaactgattt
      781 cgcacgtctg tccgaaaggt ttttattgta tttatcatca gcgcggaagt cggtattggc
      841 tgtaatcaat atcgtaatgg ttggattttc gtctaaagtt ttaaccaagc catctaaagc
      901 ggttgatgac tccggacgca gtgtggcttt atttaaatca taaaatactt ctggtaaatc
      961 aattggcctg gctgtgcttt ttaatgggaa gtcaaaatct ttact
//
LOCUS       DAOMAA010001054         1005 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_215912_length_1005_cov_2.484211, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010001054 DAOMAA010000000
VERSION     DAOMAA010001054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_215912_length_1005_cov_2.484211"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..>1005)
                     /locus_tag="PLO59_11785"
     CDS             complement(67..>1005)
                     /locus_tag="PLO59_11785"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HQV01838.1"
                     /translation="EPDAAEILPVQFDYIQQYVDSFETAMASLQFFNLTTGWRKYADD
                     ESFMDYFFINELSKNVDGYRLSTFFHKDKDSKGGKIKMGPPWDYNLAWHNADYCNNET
                     TTGWAYRITDNCSADLSFWWKRLMLDSQYKNKMKCRWLTLRTTVLDTTNIFGMMDSMV
                     NYIGVAANRHFNTWQILGSYVWPNPSPLANTYAEEIQQTKNWIAARLTWMDNNLPGIC
                     NPAGITEVPKIEVITYPNPVTDALHFNYATAHTRSLQIKIYNAYGACVLIKPLQSIAY
                     QDIAYNIPVNHLHAGMYVLQLIENDFVVSNSKFVKQ"
BASE COUNT          312 a          193 c          175 g          325 t
ORIGIN      
        1 gtttaaatcg aaatggcgat ttaagtaacg gatacagact taaacaaaaa tcatgtataa
       61 tttactttat tgctttacaa acttgctgtt gctaactaca aaatcgtttt ctattaattg
      121 taaaacatac ataccggcat gcaaatggtt aacaggtatg ttatacgcaa tatcctgata
      181 agcgatactt tgcaatggtt taatcaaaac acaggcacca taagcattgt aaatttttat
      241 ttgcaaactt ctagtatggg ctgtagcata gttaaaatgc aatgcatccg ttaccggatt
      301 gggataagtt attacttcaa tcttaggtac ttcggtaatg cctgcagggt tgcaaatacc
      361 cggcaaatta ttatccatcc aggtaaggcg tgcagcaatc cagtttttgg tttgttgtat
      421 ttcttcggca taggtatttg ccaatggaga cgggtttggc caaacatagg agcccaatat
      481 ttgccaagta ttaaaatgcc tgttggcagc cacaccaata taatttacca tcgaatccat
      541 catgccaaaa atgttggtgg tgtctaaaac tgtggttctt aatgtaagcc acctgcattt
      601 cattttattt ttatattgac tatccaacat taaacgtttc caccaaaacg ataaatcggc
      661 actacaatta tcggttatac gatatgccca accggttgtg gtttcgttgt tgcaatagtc
      721 tgcattgtgc caggctaagt tataatccca gggaggtccc attttaattt tgccgccctt
      781 gctgtcttta tctttatgaa aaaaagtaga aagcctgtat ccatcaacat tcttacttaa
      841 ttcatttata aaaaaataat ccataaaact ttcgtcatcg gcatatttac gccaaccggt
      901 tgttaggtta aaaaactgca acgaagccat ggctgtttca aaactatcta catactgttg
      961 tatataatca aattgaacag gcaatatttc agctgcatcc ggttc
//
LOCUS       DAOMAA010001055         1004 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_216421_length_1004_cov_1.997893, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010001055 DAOMAA010000000
VERSION     DAOMAA010001055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_216421_length_1004_cov_1.997893"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="PLO59_11790"
     CDS             <1..693
                     /locus_tag="PLO59_11790"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HQV01839.1"
                     /translation="SLQVHLKEASYALLHPRLQKTIDYFVDDDTHYLIKEYIPGQTLR
                     QSMAQKQQPAYYYKIIVDVLEVLNSLHRHQIFHCDIKPENILMGNQVYVLDLGLAFNR
                     DVDTHCQSNFALGYAAPELMLQQPSFINAGTDVFALAMCLYHCLTGSNPFFHQHPVYA
                     MQLQLNLPLPPHKKINKNLLAVLHKATYRQSFGRPPHQLTAAAINEVLQINNQKRYQS
                     VEEFTAALLPCF"
     gene            836..>1004
                     /locus_tag="PLO59_11795"
     CDS             836..>1004
                     /locus_tag="PLO59_11795"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019942485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HQV01840.1"
                     /translation="MSFDKLPLIAPIKKALQTQGYTTPTPIQAQAIPLILERNDILGC
                     AQTGTGKTAAFA"
BASE COUNT          318 a          196 c          178 g          312 t
ORIGIN      
        1 tcgttacagg tacatttaaa agaagccagc tatgcattgt tgcatccgcg attacaaaaa
       61 accattgact actttgttga tgatgataca cattacttaa taaaagaata tataccgggc
      121 caaacattac gtcaatcaat ggctcagaag caacagccgg cttattatta caagattatt
      181 gttgatgtgc tggaagtatt aaattcacta caccggcatc agatatttca ttgtgatata
      241 aagcctgaaa atatattaat gggcaaccag gtatatgtgc ttgatttagg tttagctttt
      301 aatagggatg tagatacaca ttgccagtca aattttgcat taggttatgc cgcaccggag
      361 ttaatgttgc aacaaccctc atttataaat gcaggtactg atgtgtttgc tttagcaatg
      421 tgtttgtatc attgtttaac cggtagcaat cctttttttc atcagcatcc ggtatatgcc
      481 atgcagttgc aattaaacct gccattgcca ccgcataaaa aaataaataa aaacttattg
      541 gctgtattac acaaagccac ctaccgacaa tcgtttggca gaccaccaca ccagttaacc
      601 gcagcggcca ttaacgaagt attgcaaatt aacaatcaga agcgctatca gtcggtagaa
      661 gaatttaccg ctgcattgct tccatgtttt taattttttg catgtccgtt tttaggcttg
      721 taataaagtt tgaaatgtgc atttgtattg cataatattt cattgaatta ataaatgata
      781 accttgcagt tttactttta tataaaatgc agttgtaatg ctgctttaat ttatgatttc
      841 atttgacaaa ctacccttga tagcgcctat taaaaaggca ttgcaaacgc aaggatatac
      901 cacacccaca cccatacagg cacaagccat accgttaatt cttgagcgaa acgatatttt
      961 gggctgtgca caaaccggta ccggtaaaac ggctgcattc gcta
//
LOCUS       DAOMAA010001056         1003 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_216686_length_1003_cov_2.901899, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010001056 DAOMAA010000000
VERSION     DAOMAA010001056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_216686_length_1003_cov_2.901899"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="PLO59_11800"
     CDS             <1..321
                     /locus_tag="PLO59_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQV01841.1"
                     /translation="SIYKLPLSPQVKTYRKLFHLLSYEPHITTKQLQSIHCPTLVIGG
                     DHDVINTRHTLLIAESIPKSYLWILPNSGHSTPIVYANMFNEVVNDFFKNPYRKIEGT
                     GRLQ"
     gene            365..>1003
                     /locus_tag="PLO59_11805"
     CDS             365..>1003
                     /locus_tag="PLO59_11805"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HQV01842.1"
                     /translation="MSIEKTDEGLERVVGVSGLALNIVNFTIGAGIFVLPAVVGIQLG
                     AFSIFSYIFCAIVLATIMLCYAETGARITSSGGSYTYVLTAFGELPGFIINWLFFLGW
                     GILGDAALLNIITDSLAVIFPVFINPWLRAVLFFVLLSFMILLNIRGAKQGINFIKIV
                     TLIKLIPLLAIIIFGFGYIKTENLYWEHIPSLKTFADTSLVLFFAFAGFETAL"
BASE COUNT          293 a          186 c          175 g          349 t
ORIGIN      
        1 tcaatctata aattaccctt atcgccgcag gtaaaaactt accgcaaact atttcattta
       61 ctttcgtatg agccacatat cacaacaaaa caattacaat caatacactg ccctacttta
      121 gttattggtg gcgatcatga tgtaattaat acaaggcata cactgttaat tgcagaatca
      181 atcccaaaat catatttatg gatactgcct aacagtgggc actccactcc cattgtgtat
      241 gcaaatatgt ttaatgaagt ggtaaatgat tttttcaaaa acccatatcg taaaattgaa
      301 ggcaccggac gcttacagta aaatagcttt taaataatta tcgctcaaca aaaactatca
      361 ttacatgtca attgaaaaaa cagatgaagg cttagaacgg gttgtaggcg tatcgggcct
      421 tgcactcaat attgtaaact ttactattgg ggcaggtatt tttgtattgc cggctgttgt
      481 aggtattcag ttaggcgcat tcagtatatt cagttatatt ttttgtgcta tagtgttggc
      541 tacaatcatg ctatgctatg ccgaaacggg tgcccgtata accagcagtg gcggctctta
      601 tacttatgta ttaacagcct ttggcgaatt accgggtttt attattaact ggttattctt
      661 tttgggttgg ggtattttag gcgatgctgc tttactcaac atcattaccg attcattagc
      721 cgtaattttt cctgttttta taaacccatg gctacgggct gttttgtttt ttgttttact
      781 gagttttatg atattgctaa acatacgagg cgctaagcaa ggtataaact ttattaaaat
      841 agttacactt ataaaattaa taccgttact cgccatcatc atttttggtt ttggatatat
      901 taaaactgaa aatctctatt gggagcatat cccatcgcta aaaacatttg ccgacacatc
      961 ccttgtttta ttttttgctt ttgccggttt tgaaacagca ctt
//
LOCUS       DAOMAA010001057         1003 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_216701_length_1003_cov_2.803797, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010001057 DAOMAA010000000
VERSION     DAOMAA010001057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_216701_length_1003_cov_2.803797"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..>1003)
                     /locus_tag="PLO59_11810"
     CDS             complement(136..>1003)
                     /locus_tag="PLO59_11810"
                     /inference="COORDINATES: protein motif:HMM:NF016338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl transferase"
                     /protein_id="HQV01843.1"
                     /translation="DVSTIKLLYQIPFLPIELFKYFDVINETAPQQNVQTFISSGTTG
                     SKNSRHIVADANLYVQSFTKGFELVYGKPGQYCFLCLLPSYTQRGNSSLVYMMHYLMQ
                     KNKHRATGFYQNADKNFINTLLALQKSKQPTIIVGVTYALLDLVALMQKQKIRMDESI
                     TIIETGGMKGRRKEMIREEVHQHLKSVFAGNIHSEYGMTELLSQAYATANGLFKTPPW
                     MKILIRDANDSSHYKSAGSMGAINVIDLANVYSCAFIETQDIGRMHTDGSFEVLGRMD
                     ASELRGCNLMFA"
BASE COUNT          295 a          212 c          172 g          324 t
ORIGIN      
        1 aaaatagacc ccagtgtaag ccagtcgttt gcagtaagtg tgcccccgcg tttgagttta
       61 aacataaaaa aactcatcag cgacccaacc gttatagagc ctattagtaa agtgtatctt
      121 cttttattag tcattttatg caaacattaa attacaacca cgcaattcgc tggcatccat
      181 acgtcccaaa acttcaaagc tgccatcggt atgcatacgg ccaatatcct gcgtttcgat
      241 aaaggcgcaa gaatacacat ttgccaaatc aattacatta atagcaccca tactacctgc
      301 gcttttgtaa tgactgctgt cgttagcatc acgaattaaa attttcatcc agggcggtgt
      361 tttaaacaag ccatttgccg ttgcataagc ttgcgataaa agctcagtca taccatactc
      421 gctatgtatg ttacctgcaa aaacgctttt caagtgctga tgtacttctt cgcgaatcat
      481 ttcttttcgc ctgcctttca taccgcctgt ttcgataatg gtaatacttt catccatacg
      541 tattttttgc ttttgcataa gcgcaaccaa atctaataat gcataagtta cccccacaat
      601 aatggtgggc tgtttgcttt tttgtaaagc aagcaatgtg tttataaaat ttttatcagc
      661 gttttggtaa aagcctgttg cacggtgttt gtttttttgc attaagtaat gcatcatata
      721 caccagcgat gagttgccgc gttgtgtata agatggcaat aagcataaaa aacaatactg
      781 cccgggcttg ccatacacca actcaaaacc tttggtaaaa ctttgcacat acaagtttgc
      841 atcggcaact atatgcctgc tgtttttact tccggttgta ccactgctaa taaatgtttg
      901 cacattttgt tgtggcgctg tttcattaat tacatcaaaa tatttaaaca attcgatggg
      961 taaaaaagga atttgatata atagttttat ggtgctaaca tct
//
LOCUS       DAOMAA010001058         1002 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5144
            NODE_217208_length_1002_cov_2.504752, whole genome shotgun
            sequence.
ACCESSION   DAOMAA010001058 DAOMAA010000000
VERSION     DAOMAA010001058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867303
            Sequence Read Archive: SRR5580753
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,363
            CDSs (total)                      :: 2,353
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 4 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_217208_length_1002_cov_2.504752"
                     /isolate="AMR_MDS_5144"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="PLO59_11815"
     CDS             complement(<1..749)
                     /locus_tag="PLO59_11815"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQV01844.1"
                     /translation="MKLNFKNSILVITTLLLTNNVWAQTKSLTLKDLLKIGLDQSYDI
                     QVSKLEEALVEKQITETRGLTLPQINGNGSVNNNYKRQVLVLPAGLGGGTSEGPNKIE
                     AGTAYSSVVGVEATQALIDVAALEGLKAAKAGRDYARVNTQQTKEQVVSTIAQQYYQI
                     LTSMEEAKLQQHTIEIMQQLVQASEGKYENGLLRKVDVDRIKVNLINAQSRLTQAQND
                     VALKTNQLKVTLGLPYATQLVLDTLDVSNIV"
BASE COUNT          257 a          191 c          189 g          365 t
ORIGIN      
        1 ataacaatat tcgaaacatc caaagtatct aataccaatt gtgtagcata aggtaaaccc
       61 agtgttactt taagctgatt ggtttttaat gcaacatcgt tttgagcttg cgttaagcgc
      121 gattgtgcat ttataaggtt tacctttatc ctatctacat ccaccttacg taaaaggcca
      181 ttttcatatt tgccttcaga cgcctgtacc agttgctgca ttatttcaat ggtatgttgt
      241 tgcaactttg cctcctccat actggttaat atttgatagt attgttgagc tatagtgcta
      301 accacttgct ctttggtttg ttgtgtgtta acacgtgcat aatcgcggcc tgctttggca
      361 gccttcaacc cttcaagtgc agctacatca ataagcgctt gtgtggcttc tacgccaact
      421 acagagctat aagcggtacc ggcttctatt ttattggggc cctcactggt accgccaccc
      481 aaaccggccg gtaaaaccaa tacctggcgt ttgtaattat tgtttacgct accgttgcca
      541 tttatttgtg gcaatgtaag gccgcgtgtt tctgtaattt gtttttcaac caatgcctct
      601 tctaatttgc ttacttgtat atcatagctt tgatctaatc cgattttaag caaatctttt
      661 aatgttaatg acttggtttg tgcccaaaca ttatttgtta gtagcaaggt tgtaattacc
      721 agtatgctgt ttttaaaatt tagtttcatt actatttgaa tttttatttg gttgcctaac
      781 aagggttata ctatagcttc ttgaaagtga gaccaaaatt gcaggtttta gcagtgtgca
      841 tagtttgctt tataacctaa acagtaaaat tcattactga aacggagtat tagcctaccg
      901 aactttttaa ggtggtttag cttattaaat ccaataaatg gttgccaaaa aggtttcgga
      961 tgtttaaaat gctgcttcgg tttgcaattt ggcttgttgg gt
//
